LOCUS       DAOQVW010000001        12060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_5967_length_12060_cov_3.429071, whole genome shotgun sequence.
ACCESSION   DAOQVW010000001 DAOQVW010000000
VERSION     DAOQVW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5967_length_12060_cov_3.429071"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..542)
                     /locus_tag="PKK85_00005"
     CDS             complement(102..542)
                     /locus_tag="PKK85_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2120 domain-containing protein"
                     /protein_id="HNS24438.1"
                     /translation="MRTHNIASVIMGSLEAFEGSRAAMDTSQMLIVRGMSRKRVQPNE
                     LDSILDQLIKDLGMTEMDLYSEEAGDIVGIMDEQIRASVPIQSETDIAGIFRLKESFE
                     AMNCHTDYRLGLIEGDIAVFVVIWKDKSGIGPLFVEVVVSLLEA"
     gene            complement(539..1477)
                     /gene="mptA"
                     /locus_tag="PKK85_00010"
     CDS             complement(539..1477)
                     /gene="mptA"
                     /locus_tag="PKK85_00010"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HNS24439.1"
                     /translation="MPDTQNKTPSVPVHLTRVGVTGVKKLLKIERVGKRPIVLIPTFD
                     AFVDLPSTQRGIHMSRNPEAISEVLEEALDKTTVEIESLCASIVHSLLKKHKYATHAE
                     VSMKSDFMIMKKSPVTRLKTQEMTKIMADASGIRGEDGEVVIRKMIGAEVVGMTVCPC
                     AQETVKETSKQKLLKFLDHETTEKVLDTVSFASHNQRGIGMIMIEVPEHQIIRGEDII
                     EIIEGSMSSSVSEMLKRPDENAVVLHAHRNPMFVEDCVRNMVQKIVHQYSHLPDDTLI
                     TVRQVNEESIHRHNAFAEKVATLKELKSEIEEGNEL"
     gene            complement(1669..2316)
                     /locus_tag="PKK85_00015"
     CDS             complement(1669..2316)
                     /locus_tag="PKK85_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2100 domain-containing protein"
                     /protein_id="HNS24440.1"
                     /translation="MLKEAGKNINTGDGLKKPREGQINSRVHGEIMNLLLESEEFIYS
                     SRPDHHLLEDEAQLFCGKLLEIREKIDSLLVDFGVMEVENLEDEVSKLSSQFIFLTPK
                     ANFKKSLVHLGVDPQRIVVAGVPLQIEDMQILNPKIPEKALKSINIKIEHVKNDLERK
                     IQLFNPKNVLVVIEKDKAGEILAKRAEKLFNASKIEKESIKNMEIHDFSMLLKNC"
     gene            complement(2359..3384)
                     /locus_tag="PKK85_00020"
     CDS             complement(2359..3384)
                     /locus_tag="PKK85_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNS24441.1"
                     /translation="MMALVYSPEYLEHKTSNHPESPDRLIKIMEGLDEADLLRKIPIF
                     EPCMATEEDIERVHTSDHVEKIRTFCESGGGYLDFDTYASPQSYEIAKLAAGGVIKAS
                     ELVLDNFSSAYALVRPPGHHATRERAMGFCLFNNLAVALEYLRKVRKINKFIIFDFDV
                     HYGNGTANIFYNDPDVLYISIHQDPHTIFPSQGFLEEMGGGPGRGSNLNIPLSPGSGT
                     PDYIYILERILRPVFKEFNANFLFFDVGFDAHKDDPLSRIFLDDDFFEWMAMEILNQP
                     QPRVLTLEGGYHLEAMKRCNLKMINLLKEERLEHSPISPDMTEVRDETKKLYRHIEDN
                     FSPFFTF"
     gene            complement(3444..3923)
                     /locus_tag="PKK85_00025"
     CDS             complement(3444..3923)
                     /locus_tag="PKK85_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNS24442.1"
                     /translation="MKGSDLPDTVKIDDLDRDIISIFNKDGRISYRKIAKKLDISIGT
                     VHNRMEKLIRNGVIKKFAPVIDHQKLGYNLTTIIGVRVKGGVLRNWEDRTAYHHNVLC
                     MYDVTGEFDAILIARFKDTNDLDTFIKKLLKEPDVQRTYTQTVLNIVKEDLSSIKLL"
     gene            complement(4450..5133)
                     /locus_tag="PKK85_00030"
     CDS             complement(4450..5133)
                     /locus_tag="PKK85_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNS24443.1"
                     /translation="MDIGDIISDSIKYPSSNWGKVLILGVIMIASILIVPIFIVYGYV
                     FRIIKATLAGLDELPEFDEIGEMFVDGLKLFVVAIVYAIPVWILSWILSAALGSGITM
                     SYTTSLDPTMMWGLFLGNIVVAILAVIIGLIEIMAIANMAYYDGDMGAAFRFSEILDY
                     ISRIGWGKYIATYIAIAIVAFIGFLIGMLTMFILIGIILLPLIIAPYIAMFGSRAIAL
                     LFIDGVAEE"
     gene            5549..6238
                     /locus_tag="PKK85_00035"
     CDS             5549..6238
                     /locus_tag="PKK85_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24444.1"
                     /translation="MKWQFIIVILLSIFGVFLFFSALLGPVDPVGRLGFVKFANPDMF
                     PGHPHSEVLADYGKQRGSKCVLVVHYAGDSNYRHYTENNVTIIELAFVDKVNRAKTDI
                     DWNDVLQTFIYGIPKDRWKYKADGKIFDDYDEAMAYVRKIARQKGQQGPIPMVYHGTA
                     RAGNPIVNQGCGFPLYVQICWYEYGRWAAYYYIIKGMIQPYLSLPYRTFEWMHASELQ
                     YYYTQGMLNYE"
     gene            complement(6269..7444)
                     /locus_tag="PKK85_00040"
     CDS             complement(6269..7444)
                     /locus_tag="PKK85_00040"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004809 - tRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HNS24445.1"
                     /translation="MDHVLINEGLVHIRIPPFEKVSSRAPVFYNPVMELNRDLSVLAL
                     QQFKSEIEKGLSICDAFGGSGIRGIRYGKEVSGVDGVVINDVNPLAVSLARDNSLENG
                     LDNVTVCQMDANLLLRLCKGKFNVVDIDPFGTPSPFIDSVAVNLPRNSMLAVTATDTS
                     ALCGTYPEPCIRKYGSNSLKTEYCHENGLRILAGYLSLSFARYRKFLTFKFSHSTEHY
                     LRLYATISKGAKKTDKSLKNMGYIAHCPSCLNRQVYRGMAPIVDVKCTRCNHLMRVAG
                     PLWCGDIQDSDFISGMIEKSSELVLNQEKNAIKLLELCKAESGAPPTFYDIHTICSKL
                     KISAPPLKDLLDRLKAEDHMATPTHFTPTGIKTSAPLEVVESLVEDLVKKQKFYPEK"
     gene            7556..8473
                     /locus_tag="PKK85_00045"
     CDS             7556..8473
                     /locus_tag="PKK85_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNS24446.1"
                     /translation="MSQLPYLENAKTGKNNWWRYVLTTVGSIGLASIAAGLLLALIIV
                     VYTVLYYPIISPDMINSLISVNSHLFLVLITGVTYALSFFLFYLFVHFLHRKNFRILI
                     NYGVGFRWRMFFKGGFIWLVIVSLLSFPDLFLNPSAYQVSLNPESFYLLLVLSALAFP
                     VQTSFEEILFRGYLMQGISLISKGPVIPLLVTSAMFGLVHFFNGTNLDQSLSIVISTF
                     LIGLMLGIITLVDNGLETAMGVHLVNNLFVALIFNSADSGLPGLPSLITAPASKPCTG
                     IFFLILAVVLMLLVLYWNRKQELGAIFRI"
     gene            8674..9768
                     /locus_tag="PKK85_00050"
     CDS             8674..9768
                     /locus_tag="PKK85_00050"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNS24447.1"
                     /translation="MIPIAKPLIGEEEIDNVVKVLKSGMIAQGPRVAEFEEKFAEYVG
                     VDHAVAASSGTTALHLAILAAGIGKGDEVITTPFSFVATGNCILYAGACPVFVDIDPL
                     TYNINPSKIEEAVTDKTRALLPVHLYGQPAQMDEINRVARDNDLVVIEDAAQAHGALY
                     NNEKTGSLGDMGCFSFYPTKNMTTSEGGIITTDNEEIADNARILRAHGEKERYSHVVL
                     GYNFRMTDIAAAIGLSQLKKLDGFNQRRQENAEYLTEHIKEIPGIQHPHVLAGAEHVF
                     HQYTIRVGGGKREEFMKFLNENGVGTGIHYPITLYQQKLYRDLNFDADCPESEKAANE
                     VLSLPVHPNLSVEDVEKIVNVISTAANELF"
     assembly_gap    10281..10290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10291..10587)
                     /locus_tag="PKK85_00055"
     CDS             complement(<10291..10587)
                     /locus_tag="PKK85_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNS24448.1"
                     /translation="MICAELTIIPIGTSETSLSRYIAAAITALEDTGIKYELNGMGTQ
                     LESETPDELFDAIKKAHEAMFHEGVLRVATSIKIDDRRDADKGLSQKILAVKEKL"
     gene            10623..11489
                     /locus_tag="PKK85_00060"
     CDS             10623..11489
                     /locus_tag="PKK85_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HNS24449.1"
                     /translation="MRSLNKETFNQGLLNRIEKVITDYSLIDPDEKVAVALSGGKDSV
                     LTLHALYNFREIFNFELVAIAIDEGISGYRADGLQIARQHTSELGIELIEKSLKTEMD
                     ITLDQATNFYQTACIPCGVFRRYLLNRTANDLEVDKLATGHNLDDEIQSFLMSFARAD
                     TRRFAKFGPKLDRIHPRMIPRIKPLWKIQEKEVGHWVVVNELEVHMAECPYAHTSFRS
                     YLKNYLNQLEEKHPGTKMNILKSFQKSFQFRKLPQMGIELQECQRCREPSSSVLCKAC
                     EIREEIESNRKK"
     gene            complement(11626..11841)
                     /locus_tag="PKK85_00065"
     CDS             complement(11626..11841)
                     /locus_tag="PKK85_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HNS24450.1"
                     /translation="MYIIFRCNCGRVNYSKKGFKTKKCVCGKNLKVKNMRIIKKVQTH
                     QEASEIVQQLQEEIHGGAIFTTADKIR"
BASE COUNT         3484 a         2470 c         2391 g         3705 t
ORIGIN      
        1 ctttttccat aaagaatggt atttgatccc catcggcact taaaatggca acaacatctt
       61 ttttagaaat tgacatcttg gtcttcctcc ctctaaggaa gctaggcttc caacagggat
      121 actactacct ccacaaacaa aggaccaatt ccgctcttat ccttccaaat caccacaaaa
      181 acagctatat ctccttcaat aaggcccaat cgataatcgg tgtgacagtt catggcttca
      241 aatgactctt taagccgaaa tattcctgca atatccgttt cactctgaat tggtactgag
      301 gcacggatct gttcatccat gatccccaca atatctccgg cttcttcaga gtataaatcc
      361 atctccgtca ttcccaagtc tttaattaac tggtcaagga tggaatccag ttcgttgggt
      421 tgcactcttt tccgactcat gccccggaca atcaacatct gggaggtgtc cattgcagcc
      481 cttgaacctt caaaggcttc taagctaccc attattacac tagcaatgtt atgagttctc
      541 ataattcatt accttcctcg atttcacttt taagctcttt taaagtagct actttctcag
      601 caaaggcatt gtgccgatga atactttcct cgttcacctg cctcactgtg atgagggtgt
      661 catcgggcag atgggaatat tgatgcacga tcttctgcac catgttacga acgcagtcct
      721 ccacaaacat gggatttcga tgggcgtgaa gaaccaccgc gttttcatcg ggtcttttga
      781 gcatttcaga aactgacgaa ctcatggaac cttcaattat ctctataata tcttcaccac
      841 gaataatttg gtgctcaggc acttcgatca taatcatccc gatacctcgc tggttgtgtg
      901 atgcgaaaga aacggtgtcc aacacttttt cggtagtttc atggtccaaa aattttagaa
      961 gcttttgctt ggaagtttct ttaactgttt cctgagcaca gggacagact gtcataccta
     1021 ccacttcagc accgatcatc ttccgaatta ccacctcgcc atcttctcct cggataccac
     1081 tggcatcggc catgatcttg gtcatctcct gggttttaag acgagtgacg ggtgattttt
     1141 tcatgatcat gaagtcactt ttcatgctca cctctgcgtg ggtggcatac ttatgttttt
     1201 tcaggagcga atggactata ctcgcacata aagattcgat ttcgacggtg gttttatcaa
     1261 gagcttcttc aagaacttca cttatggctt cagggttacg ggacatgtgg atacctctct
     1321 gtgtgctggg aagatccaca aaagcatcaa aggtgggaat tagaactatg ggtcgtttcc
     1381 ccaccctctc gatctttaaa agttttttta ccccggtaac tcccactctg gttaaatgaa
     1441 cgggtacgct aggtgtctta ttttgagtgt ctggcatgca tgttaggtcc aatttctttc
     1501 caccccttat atcatcctct aactctaagc tcgaagatca aaaagcaact ttttcatagt
     1561 cttataatta acaggattgg tgttatacat aagaaactat ttcacctatt atatcttttg
     1621 tgatatgctc ttgatgtgga gtaactgtgc acaaaatcca tcataaaatc agcaattctt
     1681 aagcaacatg gaaaaatcgt ggatttccat attctttata ctttcttttt ctattttaga
     1741 ggcattgaaa agtttttcag ccctttttgc taggatttcg ccagctttat ctttttctat
     1801 cacaactaaa acattttttg ggttgaataa ttggattttt cgctctaaat catttttaac
     1861 atgttctatt ttaatattta ttgattttaa agctttttca ggtattttgg ggtttaggat
     1921 ttgcatatcc tcaatctgaa gcggcactcc agctaccaca atcctttgag gatccactcc
     1981 caagtggacc aacgattttt taaagtttgc tttgggggtt aagaaaataa attgtgaaga
     2041 gagcttagac acttcatcct ccaaattttc aacctccata actccaaaat caaccagtaa
     2101 actgtcaatt ttttctctaa tctctaggag cttcccacag aatagctgtg cctcatcctc
     2161 aagtagatga tggtccgggc gggaagagta aataaactct tcagattcca acagcaagtt
     2221 cataatctcc ccgtgaactc gggagttaat ttgtccttct cttggctttt ttaaaccatc
     2281 accagtattt atgttttttc cagcctcttt taacaaagtt tgggcctgtt ttagtcttaa
     2341 ctgttccatc aaatctcctc agaacgtgaa aaatggtgaa aaattatcct cgatgtgtcg
     2401 gtatagtttt ttggtttcat cccgaacttc tgtcatatca ggagaaattg gggaatgttc
     2461 taatctttct tccttcaata gattaatcat tttcaggttg caccgtttca tggcttccag
     2521 atggtaaccc ccctccaggg ttagaacccg tggttgaggt tgatttaaga tttccatagc
     2581 catccattca aaaaaatcgt catccagaaa aatccgggag agcggatcat ccttgtgggc
     2641 atcaaagccc acatcgaaaa agagaaaatt ggcattaaac tctttaaaaa ctggtcgtaa
     2701 tattctttcc aggatatata tatagtctgg agtccctgaa ccaggtgata ggggaatatt
     2761 caggttggaa cctcttcccg gccccccgcc catctcttct aagaaacctt gtgatgggaa
     2821 gatggtgtgt gggtcctggt gaatggagat gtacaggaca tccgggtcgt tataaaaaat
     2881 attggcagtt ccattaccat agtggacatc gaagtcgaat attataaatt tattgatttt
     2941 tctaactttc cgaagatact ccagggccac ggcaagattg ttaaacaaac agaaacccat
     3001 ggcacgttct ctggtggcgt gatgacccgg aggccttacc agtgcatatg cactgctaaa
     3061 attatcaagt accagttccg atgcttttat aactccacct gcagctagtt tggctatttc
     3121 atagctttgg ggtgaggcgt aggtgtcaaa gtccagataa cctccgccac tttcacagaa
     3181 ggtcctgatc ttttcaacgt gatccgaagt gtgtactcgc tctatgtctt cttcggtagc
     3241 catgcatggt tcaaagatgg gaatttttct cagcaaatca gcttcatcta ggccttccat
     3301 tatttttata agtcgatctg gggattcggg atggttggaa gttttatgtt ccaagtattc
     3361 gggtgaataa accagggcca tcatttatgc tatctccatg tcacagtttt tggaagtctt
     3421 tcattgctgt agatggaatc ctgctaaagt agctttattg aacttaaatc ttccttcact
     3481 atatttaaaa cggtttgggt gtaggttctc tgaacatcgg gctcttttaa gagcttttta
     3541 atgaacgtat ccagatcgtt tgtgtcttta aaccgggcaa tgagtatagc atcaaattcc
     3601 ccggttacgt catacatgca cagcacgttg tggtgatagg cagtgcgatc ttcccagttt
     3661 cttaaaactc ctccctttac cctaactccg atgattgtgg taaggttgta acccaatttt
     3721 tgatggtcta ttaccggtgc gaattttttt atcactccat tccggatgag tttttccatg
     3781 cggttgtgta cggttccaat ggatatgtcc agttttttgg cgattttacg gtaggaaatt
     3841 cttccatcct tgttgaatat gctgataatg tcacggtcca ggtcatcaat tttcactgta
     3901 tccggcaagt cacttccttt catacaattc cacctttaca tgaacaatga ttatattatg
     3961 gtacttatta aattattata acatacaatt tttattgttg ccttaatgtt gacccgtcaa
     4021 tacactggcc tgggttgtta tagatgcatt gcaaatgaca tccccgatcc aggttttttt
     4081 ttaaatggca tgcttcaaag attacagtct atggggagac tctcaaaaat ccctagttgt
     4141 caaattaggt ccgggatgta cacattcagc tgtgttaggc tgctaaaaaa cagtgacgca
     4201 tgtgatcttt gtggatggac ttaggaggag aaaaacaagc aacaccgtct ggggtggttg
     4261 aacgcgtgaa tattcgtttt agcggtacta gtggaagcag cacatggttt gagagaaatt
     4321 gtaataagaa aagtggattt aatcatcaac caaggtgctg gttgtttgga attgttttaa
     4381 ccagggaatt acatcccact ttatctattt agccaaacaa taaaaaaaaa tagtaaaagg
     4441 aaattttttt tactcttcag ctactccatc aatgaacaag agagctatgg ccctggaacc
     4501 gaacatggca atgtaaggag caattatcag tggcagtaga attattccta tcaggatgaa
     4561 catggttagc atccctatta ggaatccgat aaatgctaca atggcaattg caatgtaggt
     4621 cgctatgtac ttaccccatc ctatacggga tatgtagtct aggatttcgc tgaatcggaa
     4681 agcagctccc atgtctccat cataataagc catgtttgct atggccatga tttcaattag
     4741 accaattatc actgccaata ttgcgactac tatgtttccc aggaacaagc cccacatcat
     4801 ggtaggatcc aggctggtag tgtagctcat tgtaattcca gagcctagtg ctgcacttaa
     4861 aatccaagat agtatccata ctggaattgc gtaaacaatt gcaactacaa atagtttcag
     4921 gccatcaacg aacatttccc caatctcatc aaactcggga agttcatcca gcccagcaag
     4981 tgtagcctta atgattctga acacgtaccc atacactatg aaaatcggta caatcaaaat
     5041 cgaggctatc attataactc ccaaaataag tacctttccc caattggagg atggatactt
     5101 aatggagtca gatattatat ctccaatatc cattttcaat cacctctaaa cgttattaac
     5161 aaatcactat atgaagttaa tataagatat tattttcgat ggcgcacttc aaaaaaacag
     5221 tatttaaaaa aagggattga taatattatt cttattttta taaaaccatt accatgttct
     5281 acatttccca atttatataa ataataatat ttttggatca atataaatag aaatatgaat
     5341 taattttgca gcatcaattt gaaaccatat tatttctccc aaaaaaaaga cagattatat
     5401 gcaatgtcaa cctgtcctga tcaaaacctt taaggatgag aaaagggcct tataaattat
     5461 attcgatttc ttctatgaat gtttgaatta aaaatacaac agacaataat gaatactaaa
     5521 aaggtcatct aatttataag gtgaaatatt gaaatggcaa ttcatcatag taatcctcct
     5581 gtctatattc ggagtctttt tattcttctc agccctttta ggtccagtgg acccggtggg
     5641 aagactgggc tttgttaaat ttgccaaccc tgatatgttc ccaggccacc cccactctga
     5701 ggtgttggct gactatggta aacaaagagg gtctaaatgt gtcttggtag tacactacgc
     5761 tggtgattcc aactaccggc actacaccga aaacaacgtt actataattg aactggcctt
     5821 tgtggacaag gttaaccggg ctaaaacgga tatagattgg aatgatgtgc tgcaaacatt
     5881 catatatggc atacccaagg atcggtggaa atacaaagcg gatggtaaga tatttgatga
     5941 ctacgacgag gctatggcct atgtaagaaa aatagctagg cagaaaggcc aacagggccc
     6001 tatacccatg gtttatcatg gaacagcccg ggcgggtaat cccattgtta accaaggctg
     6061 tggatttcca ttgtacgtcc agatctgctg gtatgagtat ggtcggtggg cggcctacta
     6121 ctatataata aagggaatga tacaacccta cttgagtttg ccctacagaa cctttgaatg
     6181 gatgcacgcc tcggaactac agtattatta cacccaaggc atgctgaact acgaatagag
     6241 gagttatcgg gatatcaaca taaccctttc atttttctgg ataaaatttc tgttttttaa
     6301 ccaagtcttc aactaaactc tccactactt ccagtggggc agaggttttt atcccggtag
     6361 gcgtgaaatg agtgggggta gccatgtgat cctctgcttt gagtcgatct agcaaatcct
     6421 tcagaggtgg tgcactaatt ttaagcttgc tacagatggt gtgaatgtca taaaaagtag
     6481 gtggtgctcc agactcggct ttgcataatt ctaaaagttt aatggcattt ttttcctggt
     6541 ttaacactaa ttcactgctt ttttcaatca ttcctgatat gaaatccgaa tcctgtatat
     6601 ctccacacca cagaggacct gccaccctca tcagatggtt acatcgggta catttgacat
     6661 ctacaatggg ggccatacct ctataaacct gacggttaag gcaactggga cagtgggcaa
     6721 tgtaacccat atttttgagg gatttgtcag tttttttagc ccctttactg atggtggcat
     6781 ataagcgcag atagtgttca gtgctgtggg agaacttgaa ggttaaaaac ttcctgtacc
     6841 gagcaaaaga cagggataaa tacccagcca gtatccttag accattttcg tgacagtact
     6901 ctgttttaag ggaattagaa ccatactttc ttatgcaagg ttcggggtag gttccacaca
     6961 gtgccgaagt gtcggtggcg gtcacggcca gcatactatt ccggggtaga ttaacggcaa
     7021 cagaatctat gaagggagac ggagttccaa aaggatcaat atctaccacg ttgaatttac
     7081 ccttacaaag ccttagaagg agattagcat ccatttgaca gacggtgaca ttgtccaaac
     7141 cattttcaag ggaattatcc ctggctagtg acactgccag tggattaaca tcatttatca
     7201 ccactccatc tactcctgaa acctctttcc cgtagcgtat accccggata ccactgcctc
     7261 cgaaggcatc gcagatactc agtccctttt ctatttcgga tttaaattgc tgaagggcca
     7321 gcactgaaag atcccggttc agttccatga cggggttgta aaaaactggt gcccgagatg
     7381 ataccttctc aaagggagga attctgatat gcaccagccc ctcatttata agcacgtgat
     7441 ccataaatta aaacccccga gtattctatc ttcatattat aatagtgttc aattaatagg
     7501 cctaataagg gtagtttata gtaagactta actaattagt ttaggagttg gtatcatgtc
     7561 ccagttaccc taccttgaaa atgcaaaaac cggtaaaaac aactggtgga gatatgttct
     7621 caccacggtg ggttcaatag gactggcgag catagctgct ggactgctat tagcactaat
     7681 aatagttgtt tacacggttt tatactaccc cattattagt ccggatatga taaatagcct
     7741 aatttccgtc aacagccatc tatttctagt tctaataact ggggttacct acgccctatc
     7801 attcttcctg ttctaccttt ttgttcactt tttgcatcgt aaaaacttta ggatcttgat
     7861 taattatgga gtgggattta gatggaggat gttttttaag gggggcttta tctggttagt
     7921 gatagtctct ttacttagct tccctgattt attcctcaac cccagtgcct accaagtgtc
     7981 tttaaatccc gaatcctttt atttgctgct ggttttaagt gcattggcct tcccggtgca
     8041 aacctccttc gaagaaatac tcttcagggg atacctaatg cagggtatca gtctcatctc
     8101 taaaggaccg gtcatccctt tactggttac atcagccatg tttgggctgg tacatttttt
     8161 taatggaact aacctggacc agagcctgtc catagtgatt tccaccttcc ttattggcct
     8221 aatgttgggg atcattaccc tggttgataa tggcctggaa accgccatgg gggtgcactt
     8281 agttaacaat ctcttcgtgg cattaatatt taactctgca gactctggcc taccaggcct
     8341 gccttcactg atcacagcac ctgcttctaa accatgtacc ggtatttttt ttctgatatt
     8401 ggcagttgtt ttgatgctgt tagtactata ttggaatcga aaacaggaat taggggccat
     8461 tttccgtata taggtttttt tttattcttt tctttttata agttgcaatg tatagcaact
     8521 gattcacaag tcaaattctt tgttgatgca acattttttt aaggcaaaga attagaatgt
     8581 atttattaaa aaagcaacaa tcaagtaatt tttcccatta ggctttataa ttttttatag
     8641 agagaatttt ttgattacaa ttcgagtgat attatgatac ccattgctaa gcccttaatt
     8701 ggtgaggaag aaatagataa cgtggttaaa gtattaaaat caggaatgat tgctcagggc
     8761 ccccgggtgg ctgaatttga ggaaaaattt gcggaatatg tgggtgtgga tcatgccgta
     8821 gcagcaagtt ccgggaccac ggctttgcac ctggcaatct tggcggcggg gataggaaaa
     8881 ggtgatgagg tgatcaccac ccctttcagc ttcgtagcca caggtaattg tatattgtat
     8941 gctggagctt gcccagtttt tgtagatata gaccctttaa cctacaatat aaatccttcc
     9001 aaaattgaag aagctgtcac cgataaaacc agggcgctac tgccagtaca tctatatgga
     9061 cagccagccc agatggatga gataaatcgt gtggcccggg acaacgattt ggtggtgatt
     9121 gaagacgcgg cacaagctca tggagctctt tataacaatg aaaagacggg ttccctaggt
     9181 gatatggggt gttttagttt ttaccctacc aaaaacatga ccaccagtga aggaggtatc
     9241 ataaccactg ataacgagga aatagctgac aatgctcgaa tactaagggc tcatggtgag
     9301 aaagagcgtt acagccacgt agttctgggg tacaactttc gcatgactga tatagcagca
     9361 gccattggat tgtcgcaact gaagaagttg gatggtttta accaacgacg ccaagaaaat
     9421 gcagagtatt taaccgagca cataaaggaa ataccgggca ttcaacatcc ccacgtcctg
     9481 gcaggggcag agcacgtgtt tcaccagtac actatccgag tgggtggggg taaacgggaa
     9541 gaattcatga agtttctcaa tgaaaatggt gtagggaccg gaattcatta tcccataaca
     9601 ttataccaac agaaattata tcgggactta aattttgatg cagattgccc tgaatcggaa
     9661 aaagcagcaa atgaggtctt atctctacca gtacatccca atttaagcgt tgaagatgtg
     9721 gaaaaaatag ttaatgtaat atcaacggct gcaaatgagt tgttttaata aataagagga
     9781 aaatttttag aatgcattta aaaaatgcat ggaggagctc ctttgggggg ggtgattatt
     9841 ggataatcac gaatggaatt cctccagcat gatgcctatt aataggcaat tattactcaa
     9901 tgttaaattt gtataaatac ttttctatcc agcaagcacg gaccattaat attgcagggc
     9961 ccacttgaac taaacttttt tcatattcag aataaggtgg ggtgacttga taattgaaca
    10021 ctaaaaaata ggcatgatgg ggcaaattta taaaatttaa ggggatttaa acgatgtaat
    10081 aattaatacc tttttaaacc ttgatatttt catctatttc ccagtctaaa tagaagttaa
    10141 aggagaatat aggtcctata aaagaatttg tttttgagtg aagaaataat acctcctatc
    10201 ttatctcagg ctatggaggt gtaaaccaag ttaactatca taaaaggtat cttaaaggcc
    10261 agtcagaaac tatttatcta nnnnnnnnnn aagtttctct ttaacagcca gaatcttctg
    10321 agataatcct ttatctgcat ctcgacggtc gtcaattttt atgctggtag caacccgaag
    10381 aaccccttca tgaaacatag cttcatgagc ctttttaata gcatcgaaga gttcatctgg
    10441 ggtttcactt tccaactgag tccccattcc atttaattcg tattttatac cagtatcttc
    10501 caatgccgta atggccgctg caatatatct actcagactg gtttcagagg ttcctatggg
    10561 aattatagtc aattctgcgc atatcatagt tttatttatg ttaactggtt catatatcat
    10621 ttatgagatc cctgaacaag gagaccttca accagggtct tttaaacagg attgaaaagg
    10681 ttatcaccga ctattccctt atagatcctg atgaaaaggt tgctgtagcc ctttctggtg
    10741 gtaaagacag tgtacttact ttacatgcac tttataattt tagagaaatc tttaactttg
    10801 aactggtggc cattgccatt gatgagggta tatccgggta ccgggctgat gggttgcaaa
    10861 ttgcgcgtca acataccagt gaactaggaa tagaacttat agaaaaatcg ctgaaaacag
    10921 aaatggacat caccttggat caagccacga atttttatca aaccgcctgc atcccctgcg
    10981 gagttttccg tcgttaccta ttaaacagga ctgctaatga cttagaggtg gataaactgg
    11041 ctactggaca taacttggat gatgagattc aatcatttct aatgagcttt gctcgtgctg
    11101 acacccgacg ctttgctaaa tttgggccaa aactggaccg tatccatccc cggatgatac
    11161 cccgtatcaa gcccttatgg aagattcaag agaaagaagt gggccactgg gtggtggtaa
    11221 atgaattaga ggttcatatg gcggaatgtc cctacgccca cacttctttt cgttcatatt
    11281 tgaagaatta tcttaaccag ttggaagaaa aacatcctgg taccaagatg aacatattaa
    11341 aatccttcca gaaatccttt caattcagaa aacttccaca aatgggaatt gaacttcagg
    11401 aatgtcaacg atgcagagaa ccatcatctt cagtgctttg caaagcctgc gagataagag
    11461 aagaaattga aagtaacaga aagaaatagg ttttttctaa ttagggaatg ccggagacag
    11521 aatcaaaaag cctaactctt attggataag ttaaagctcg acaaatccgt ataaccttga
    11581 ctactataga tgataataga tttattatgt tcacctttat tatgttcacc gaattttatc
    11641 ggcggtggta aaaattgccc cgccatgaat ctcttcttgt aactgttgca cgatttctga
    11701 tgcttcctgg tgtgtctgga ctttttttat gatacgcata ttctttactt tcagattttt
    11761 accgcaaaca cattttttag tcttaaaacc ttttttagag taattaaccc gtccacagtt
    11821 acatctgaag ataatgtaca tgatatcagc ccaaacttgt tgatattagg ttttgaaagc
    11881 atggatcact ttaagaacga tgattgaata ggttcacatc actataaata aattgttacc
    11941 ataaatgggg gttgaagatc ctaccaacca attaactgac taaaaagggg ccatatagcg
    12001 tttggtatgg ataacagggt cccaatcgtg cagccgatgt tggctcccac cactaccagg
//
LOCUS       DAOQVW010000002        11855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_6090_length_11855_cov_3.413983, whole genome shotgun sequence.
ACCESSION   DAOQVW010000002 DAOQVW010000000
VERSION     DAOQVW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11855
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6090_length_11855_cov_3.413983"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKK85_00070"
     CDS             complement(<1..679)
                     /locus_tag="PKK85_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNS24451.1"
                     /translation="MIVLAPMAGITDGAFCRDISREGFDMVTLGGYNVDPPTIQAGRE
                     ILSRGRPEFLVEEELLIPYLEDQVKIIQEEFGGFISVNLRSVTTEPIVEVSKIRGVQV
                     VEVNAHCRQKEITNLGCGQAMLRDVSRLEEYLREITQKAKSMVSVKIRANVPGVNEVE
                     VAEAVERSGANYIHVDAMLPGSNSADLNIIRSIKENTSVFIIGNNSIKDVVSAHEMLN
                     AGADGISI"
     gene            complement(732..1487)
                     /locus_tag="PKK85_00075"
     CDS             complement(732..1487)
                     /locus_tag="PKK85_00075"
                     /EC_number="3.5.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase IIa"
                     /protein_id="HNS24452.1"
                     /translation="MIQMTLIQIDNYGPWTVTPSPRAEADLQILQSELYADIQRQFAA
                     NSGLVFFTRFDNMLAVTNGVGAEEHLRIQKSIGNRYPITVSMGIGAAETPYEAQRKAS
                     AALQKYGGAQNENRKEILAIDGMVNSDESFVQIAHIDINGITNSLTDIIPAYDTSFIV
                     NRVQHFLMKKLIEKGSLLFFIGGDNFMSPCNGLDPEGILKIIEEIEDEINIALKAGVG
                     KAPNAEKAANLADLALEEIRGGFTYKLVHVMKE"
     gene            complement(1494..2390)
                     /gene="cofD"
                     /locus_tag="PKK85_00080"
     CDS             complement(1494..2390)
                     /gene="cofD"
                     /locus_tag="PKK85_00080"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNS24453.1"
                     /translation="MITVFSGGTGTPKLLQGMVEIIDPADVTVIVNTLENEYFSGVYV
                     AADLDTVMYTLAGMINQETWYGIEGDTFVTHDTLKRMGCPEILRIGDEDRGIKIQKTI
                     LMNKHPLSEAVEIQKERLGIKSKILPMSDQDSNLTIQTTEGKMDFHQFLVEKQGRPHV
                     LDINYEQVDSAPGVIEAIKNSEMVLVGPSNPITSIGPIISTKGVMKALKNAYVVAISP
                     IIGDRPVSGPAAKFMAALGYEVSCLGVARIYQDFLDKFVIDNIDAHYKDIIEKLITEV
                     VVTQTKMNNQQDKVKLARMIFG"
     gene            complement(2453..2605)
                     /locus_tag="PKK85_00085"
     CDS             complement(2453..2605)
                     /locus_tag="PKK85_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24454.1"
                     /translation="MPVNQMETNLQAITTTIAFLEKEDSCNPELLSKLKEERDRLLRE
                     LNVHHI"
     gene            complement(2643..3407)
                     /locus_tag="PKK85_00090"
     CDS             complement(2643..3407)
                     /locus_tag="PKK85_00090"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNS24455.1"
                     /translation="MEIRLIGLPGIPLLQEGDDLGEIILESFGALDLAIEDGDVLVVA
                     ETAVAKVEGEIIDLDSVIPSKKAFEIALATGKDPKLVEAIIGASKEIIKVGPDFIISE
                     TKHGFVCANAGIDESNIELGLAKPIPSDPDASASCIRDKIESITGKKVAVVISDTQGR
                     AFREGAVGVAIGISGMDPLWDRRGETDLYGRKLQTTTIAVADELSAAASIVMGQAHEG
                     LPVVLIRGVDYFSYLQNDSSKAKSLIRPKKYDVFRK"
     gene            complement(3413..4015)
                     /locus_tag="PKK85_00095"
     CDS             complement(3413..4015)
                     /locus_tag="PKK85_00095"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HNS24456.1"
                     /translation="MYLGRILAVGSNEEGSFVAYRVSSRSFPNRITKSFPESVAVVPK
                     EGFEKDIFKSPYIAYNCIRIVDDIAVVSNGSHTDVIAEKIASGMTIRDALALSLLTMD
                     YEKDDFQTPRIAGASVLNGASYIGIVTADKVIVDKVLEGESSYIATYEHTYPQKVDFE
                     ANNSSDAAAFIMDQGKFSEFTNPVTSAAAFGSKKWEISSL"
     gene            complement(4114..4503)
                     /locus_tag="PKK85_00100"
     CDS             complement(4114..4503)
                     /locus_tag="PKK85_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012956638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNS24457.1"
                     /translation="MAIDTRAYKNKLRSKQARISLVPLIDVIFTILIFLMVTSSFQAV
                     SDSAGGKPEISDTSGSSEYYLIPMSGLHTVTVNGVNMSSYIRNSAIAVHTNVIDQGEI
                     TIKPKEGLITIVTPPGMPLEKAVSTQQ"
     gene            complement(4514..5356)
                     /locus_tag="PKK85_00105"
     CDS             complement(4514..5356)
                     /locus_tag="PKK85_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNS24458.1"
                     /translation="MIYEFFSGGVSTILEMFKSGGLITYIITIIGIYGVFYSLEKIYY
                     LRKISRVEFPQIMGVVNESMERGGSLEALREIGRFHNPISRIVAEALKIGYRNKSEVE
                     DAMERVFIVEMSRMTKGLDTIRTIIEIAPLLGLIGTVIGMWYTFKALGMNANPTAMAE
                     GIYIALITTIVGLAVAIIILPLYSYINSQIEGEIDKIEIAKKMTNWSAAEMLIAVRDE
                     VDCSIDALNDANGVLEVKPVYNNPKANIWVSINPHMLEKSIGNIVKERCKEDAHIVKS
                     KLKQ"
     gene            complement(5353..5970)
                     /gene="rnhB"
                     /locus_tag="PKK85_00110"
     CDS             complement(5353..5970)
                     /gene="rnhB"
                     /locus_tag="PKK85_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNS24459.1"
                     /translation="MKLLGIDEAGRGSVIGPLVLCGVAIDKDRLRFLERLGLKDSKKV
                     APKKRLILSRKIKKIAECHLVKISAQDIDLLRSRDVNLNQIEKIGMNQIINAAKPHIC
                     LVDSMDVKPERLTRELEEVNPEIRVIAEHKADDRYPIVAASSIVAKVERDLEIHRIRN
                     EYQDIGSGYPSDPRTIKFLQERSYQDLPDFVRRSWATVEKLRGIK"
     gene            complement(5982..7058)
                     /locus_tag="PKK85_00115"
     CDS             complement(5982..7058)
                     /locus_tag="PKK85_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNS24460.1"
                     /translation="MFDFLKNKDEKVDVKKQPLSNTLGIDLGTLNTVVAMPSGDKFDL
                     YKIPSVVAVKKEEPSYVLAVGDEAKAMLGRTPEDIVAVRPLRKGVIESIAQAESLLVY
                     AMNQGSGESPESIDRIVIGIPGDASEVEKKAVEDIGRKAGAKYVLVISEGLSAAIGAG
                     LPIAEASGTMVIDLGAGSSDIVVISLGGITDIETIRSGGDDVDTNIVEKVKELFKVEI
                     GIHEAEKAKIQVGMVHCNAEMEPLNTTVIGKSMETNKPQKVEIDSKLVADAAEPLIIK
                     LIDSLSRVLERMSPELISGVYNKTIVVGGTSQLHGLKERIYEEVGVPVEISDDPMTVV
                     AKGAAIVAAEPRALEPEVRLKAMK"
     gene            complement(7063..7692)
                     /locus_tag="PKK85_00120"
     CDS             complement(7063..7692)
                     /locus_tag="PKK85_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24461.1"
                     /translation="MIENKIKILRRAAKVSTIQDSDEIWCVIAGNEEMVNNVAYAAIM
                     DKDRVEILYREHVITKESFVTKKFDFIMLYGDINKIRDLSLICKENGGAYLKIAPYYV
                     ENEPNLFLTVGPENKIKKFVGDCEKEQMRLSFLIEDQTTGFIETDVYITDKLPTLIRN
                     TIDPLFKMADVALSTLLISAQEDQISTIKKIAKKNRLFIIDFNKILKED"
     gene            8004..8663
                     /locus_tag="PKK85_00125"
     CDS             8004..8663
                     /locus_tag="PKK85_00125"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNS24462.1"
                     /translation="MFVKGTIKKAGILLSLAFLTLFFGYTYLAFIFFAALVFLLQFFR
                     DPHRAVPEGKGLILASADGRIFNGKIDTIKTVNYDDPLMEYILEKEQRGILVSTFMSV
                     FDVHVNRVPISDRVVTTKYYPGKFKIASGSVHTVNEKNLIVIDSEYGKLGIIQISGFL
                     ARRIVSYVGPGDWVESGDRLGMIKFGSRVDLIIPTPNSEVMVSEGQKSKAGETIIARM
                     F"
     gene            8768..9490
                     /locus_tag="PKK85_00130"
     CDS             8768..9490
                     /locus_tag="PKK85_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine synthase"
                     /protein_id="HNS24463.1"
                     /translation="MHIKDYLAAPDLLSLLNASTGFLSILMLLDGYYIFSAQLMLLAV
                     IFDSLDGWVARKTKRNDHFGFGKNIDSLSDVISFGVAPGMFLCIASPNMFLPYINIVV
                     GLLIVVCGILRLSRFNVLIIDNDTISKDKFIGLPIPSTVMVLGSFYLSGIFSANIASF
                     LMVVVAVLMISTVEYPKFKGLKIVMIGALLIIASCLPQGIMSLIAYLPAKLLLVFTLI
                     YVLIVPLMELYGKLLRSGPHVR"
     gene            9480..10820
                     /locus_tag="PKK85_00135"
     CDS             9480..10820
                     /locus_tag="PKK85_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF515 domain-containing protein"
                     /protein_id="HNS24464.1"
                     /translation="MLDKILGKKDKDKKDVPDLRKEDTKSDPNIGGRIKGVVSKATDK
                     SKEKISPDDNSLKKSESKNSKKKAISKKIPRPIPKPRKKSDEKPKLKPPIEPKKKPSG
                     LSSGFGRKGPDDDQRTLVGAAVFSIILIVLVGAGYYFLVYQPYQETLGSAKQTKLNEV
                     NAYFKGPLALDPQKQTLTAEIDGAETPEQALAVDVMTPATAAWREYQSQEINAQKDPY
                     GRVMITYSAAGQKNLIMKVADAQTIVNEADASVLANMVIETPDTVAVPIIITRLQAAG
                     GLVNVGDSVDVYLRTTTPTNESNQTVNPDINNTSPSISGATVLAILRAVDSGSVDANL
                     KNAQEVAINQVTLSNSRSSSVSTNVEQLLRASASGTWDRSTVSSTLNAYGWKLSDFER
                     ASNLGELDVKYLVLLEVPRENALFLMQNSDSIALTVSTQKAPQWMIQELKSIYG"
     gene            10872..11792
                     /locus_tag="PKK85_00140"
     CDS             10872..11792
                     /locus_tag="PKK85_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24465.1"
                     /translation="MIKKATLVVAFLFTFSIFLGASWAATDQQAWVQETDSALKFTYL
                     EAVIHVKIKNNNDHVEYFKISQQYYQTTPAINWTIKWTNPAAVKMAKYINTPGDYGWK
                     IEPGQTKDVSFKLVALAPNGGPLSLYPAYIARNSSIPNQFWPILNEPGLTATWFFPNE
                     IEYLNPNLDLVSWRGKFCFWIKNMDNTGPRVEGIVRAPIVPIDSKLVFSNPKVTYLDD
                     ELPWADTAAWDVTLYPGQQKHYSYTYYWPENVTGHSTGKYKSPSVSTKSNAQDTATTV
                     PTAGTGVPYALFAVGAIIVGSGVVYAKLFR"
BASE COUNT         3305 a         2538 c         2314 g         3698 t
ORIGIN      
        1 ctatggatat accatcagct ccagcattta acatttcatg ggcagataca acatctttaa
       61 tactattgtt acctatgata aaaactgaag tgttttcttt aatagaacgt ataatattta
      121 aatcagctga attagatcca ggaagcatag catcaacatg aatataattt gcacctgaac
      181 gttccactgc ttctgccact tccacttcat taactcctgg aacattggcc cttatcttaa
      241 ctgaaaccat agatttcgcc ttttgggtta tttctcgaag atattcttct aaacgggaga
      301 cgtcccggag catggcttgg ccgcatccca ggttggtaat ttccttctga cggcaatggg
      361 cgttaacttc caccacctgc actccgcgga tcttcgaaac ctcaacaatt ggttcagttg
      421 taactgatct cagattgacc gaaataaagc ctccaaactc ctcttggatt attttaacct
      481 gatcttccag gtagggtatg agtaattcct cctccaccaa aaactctggc cttccccggg
      541 atagaatttc gcgcccggct tggatggtgg gggggtcgac attgtaaccc cccagggtta
      601 ccatatcaaa tccttccctg gaaatatccc ggcaaaaggc accgtcggta atgcccgcca
      661 tgggcgctag tactatcaat taatgcacct ataatgtggg tttgaagaaa aataaatatt
      721 tagtttatgt attattcctt catcacatga accaatttgt aggtgaaacc accccttatc
      781 tcttccaaag ctagatctgc tagattagca gctttttcag cattgggagc ttttccaact
      841 cctgctttaa gggcaatgtt aatttcatcc tcaatttctt cgatgatctt caatattccc
      901 tcaggatcaa gaccattgca gggggacatg aagttgtcac cgcctatgaa aaataagaga
      961 gaaccttttt cgattaactt cttcattaaa aagtgttgaa cacgattaac aataaaagat
     1021 gtgtcatagg caggaataat gtccgttaaa gagttggtaa tgccattgat gtctatatgg
     1081 gcaatctgta cgaagctttc atcagaatta accatgccat cgatggccaa tatctccttg
     1141 cgattctcat tttgagctcc cccatacttc tgcagggctg cactagcttt tctctgggcc
     1201 tcataaggag tttcagctgc cccaataccc atactcactg tgatggggta gcggtttcca
     1261 atggattttt gaattcggag atgttcctcg gctcccactc cattggttac tgccagcatg
     1321 ttatcgaagc gggtgaaaaa gaccaaacca ctgttagcag cgaattggcg ctgaatatct
     1381 gcgtaaagtt ctgattgcaa aatttgaaga tctgcttcgg cccgtggaga aggagtgaca
     1441 gtccagggac catagttatc aatttgaatc aaagtcattt gtatcataaa atttcaccca
     1501 aaaatcatcc tcgctaactt caccttgtct tgttggttgt tcatcttggt ctgagttacc
     1561 acaacctcag ttattagttt ttctattata tctttgtaat gggcatcgat attatctatt
     1621 acaaatttgt ctaggaaatc ctggtaaata cgggcaactc ctagacagga cacttcgtat
     1681 cccagtgcag ccatgaattt tgctgctggg ccgctaactg gtcgatcacc gataatagga
     1741 gaaatcgcca ctacataagc attcttaaga gctttcataa cgcctttagt tgatattatg
     1801 ggtccaattg aggttatggg atttgacggc cctactaaaa ccatttctga attttttata
     1861 gcttctataa ctcccggagc cgagtcaact tgttcataat ttatatccaa gacgtgggga
     1921 cggccctgtt tttccaccag gaattggtgg aaatccatct taccttctgt ggtttggatg
     1981 gttaaattgg aatcctggtc actcatgggc aaaatttttg atttgattcc taatctttct
     2041 ttttggattt caacagcctc agagagggga tgtttattca ttaaaatcgt tttttgaatt
     2101 tttatgccac gatcctcatc acctattcgc agaatttcag ggcaccccat acgttttaga
     2161 gtatcgtggg ttacgaaggt gtcaccttca attccatacc atgtttcctg gttaatcatc
     2221 ccggccaggg tgtacattac ggtgtccagg tcagctgcca cgtaaactcc tgagaaatat
     2281 tcattttcca gtgtgttcac aattacggtg acatctgcag ggtcgatgat ttccaccatc
     2341 ccctgcagta gtttaggagt tccggttcca ccggagaaga cagtaatcat tgtatcgcga
     2401 tggtcttatg taataggata attatttaat taactctttc ctattccaaa aactatatgt
     2461 gatgaacatt caattctcgt aaaagccgat ccctttcttc ttttaatttg gatagaagct
     2521 cgggattgca agaatcctcc ttctctagga aggctatagt ggtggttatc gcttgtagat
     2581 tggtttccat ctggttaaca ggcatattta cactcctttc tcgaatatta tattaaattt
     2641 agttatttcc taaatacatc atattttttt ggtctaataa gggatttggc cttcgatgaa
     2701 tcattttgaa gataactgaa gtagtcgact ccccttatta aaactacggg aagtccttca
     2761 tgggcttgac ccataacaat tgatgctgct gctgatagtt catcggcaac agcgatggtg
     2821 gtggtttgaa gttttcgacc ataaagatca gtttctcctc gacgatccca tagtgggtcc
     2881 attccggaga ttccaatggc cacgccgaca gctccttctc taaatgctct accctgggta
     2941 tccgaaatca ccacagccac ctttttacca gtgatagatt caattttatc tctgatacaa
     3001 gatgcactag catcaggatc ggaaggtata ggcttagcca atcccaattc tatattagat
     3061 tcatctattc ctgcattggc acacacaaat ccatgtttgg tctcggagat aatgaaatca
     3121 ggaccgactt ttatgatctc ctttgatgca cctattatgg cttccaccaa cttaggatcc
     3181 ttacccgtag ccaatgctat ttcaaacgcc tttttacttg gaataacaga atctaaatcg
     3241 ataatttctc cttctacttt ggccacagca gtttctgcca ctaccaacac atcgccatct
     3301 tcaatggcca aatctagagc tccaaatgac tccagtatga tttcgcccag gtcatccccc
     3361 tcctgcagga ggggaattcc aggaagaccg attaacctga tttccatatt attcagagtg
     3421 agcttatttc ccatttttta cttccaaagg ctgcggctga tgttactgga ttggtgaatt
     3481 cggaaaactt accctggtcc attatgaatg ccgctgcatc ggaggagtta ttggcttcaa
     3541 aatccacttt ctggggataa gtgtgttcat atgtggctat gtagcttgat tccccctcta
     3601 aaactttatc aactattact ttgtcagcag taactatgcc aatgtatgat gccccattca
     3661 gaaccgatgc tccagctatt cgtggagttt ggaaatcatc tttctcataa tccatggtta
     3721 aaagtgacaa ggccaaggca tctctgatgg tcataccact ggcgattttt tcggcgataa
     3781 catcagtgtg agaaccattg gaaaccacag ctatgtcatc tacaattcgg atacagttgt
     3841 atgcgatgta gggactttta aaaatgtcct tttcaaatcc ttcctttgga actactgcca
     3901 cgctttccgg gaaggatttg gtaatccggt taggaaaaga acggcttgaa actctatagg
     3961 caacgaaact accttcttca ttacttccaa cggctaatat tctccctaga tacaatttat
     4021 caccctctag attgcttttg atagacatag tgtcttagac acggtatata tagattgtat
     4081 atagatgggt ctgttggacc atgattccat aatttactgt tgagtgctca ccgctttttc
     4141 cagtggcatt ccgggaggag taacgatggt aatgagcccc tctttaggtt tgatagtgat
     4201 ttcaccctgg tctattacgt tggtatgcac tgcaattgca ctgttgcgta tgtaactgga
     4261 catgttcact ccgttcacag tgacggtgtg caagccagac atggggatca ggtagtattc
     4321 tgaggatcca ctggtatccg aaatttcagg tttacccccg gcagaatctg aaactgcttg
     4381 gaaactactg gtaaccatca aaaatattaa aatagtaaaa ataacatcaa taagtggaac
     4441 taaacttatt cgagcttgtt tggagcgtag tttgttttta taggcacggg tgtcaatggc
     4501 catgatggat tcctcactgt ttcaacttac tcttcacaat gtgagcatct tccttgcacc
     4561 tttctttaac gatgtttcca atactttttt ccagcatgtg gggattgatg gaaacccaaa
     4621 tattggcttt aggattatta taaacaggtt tcacttctaa aacaccattt gcatcattca
     4681 atgcatctat agagcaatct acttcatcac gtacggcaat cagcatttct gccgcgctcc
     4741 agttagtcat cttcttggca atttctattt tgtcaatttc accttcaatt tgactgttga
     4801 tgtaagaata gaggggtaag attataatag ccacagctaa accaacaata gtggtgataa
     4861 gcgcaatata gataccctcc gccattgcag tggggttggc attcatacct aatgccttaa
     4921 aagtatacca cattccaatt acagtaccga tcagtcccag aagaggagct atctcaatta
     4981 tggtacgtat agtatccaga cccttggtca ttcgactcat ttccacaatg aaaacgcgtt
     5041 ccatggcatc ttccacttca cttttgttac ggtaaccgat cttcaatgcc tcagccacga
     5101 tacgggaaat aggattatga aatcttccaa tttcgcgcag ggcttccaga gatccgcctc
     5161 tttccatgga ttcgtttacc acgcccatta tttggggaaa ttcaactcgg gaaatcttac
     5221 gcaggtaata aatcttttcc agagaataaa aaactccata aatccctatt atggtgatta
     5281 tgtaggttat tagacctcca cttttaaaca tctccaggat ggtactgacg ccaccactga
     5341 aaaactcata aatcatttta ttcccctcag tttttcgact gtggcccagg aacgccgaac
     5401 aaagtctgga agatcctggt aagatctttc ttggagaaat tttatggtac gagggtcgct
     5461 gggataccct gacccaatat cttggtattc gttacggatt ctgtgaattt ccaagtccct
     5521 ttcgaccttg gctacaatgg aagatgcagc aacgatgggg taccggtcat ctgctttatg
     5581 ttcagcaatt acccttattt cgggattaac ttcttccagt tccctggtta gcctttctgg
     5641 tttaacatcc attgaatcaa ccagacagat gtggggttta gcggcattga ttatctgatt
     5701 catcccaatc ttttcgattt gattcagatt cacatccctg gaacggagta aatcaatatc
     5761 ttgggctgat attttaacca aatgacattc agcaatcttt tttatcttcc gggataagat
     5821 caatcgcttc ttaggagcca cttttttaga gtctttaagg cctaatcgtt ccagaaatcg
     5881 cagcctatct ttatcaatgg caacaccaca cagtaccaag ggtcctatca cagacccacg
     5941 tcctgcctcg tctattccaa gtagcttcat ttggctatgg actatttcat tgcttttaat
     6001 cttacttccg gttccagagc ccggggttct gctgccacta tagctgctcc tttggccacc
     6061 acagtcatgg gatcatcaga gatttccact ggaaccccaa cttcctcgta aatcctttct
     6121 ttaaggccat gaagttgtga agtaccaccc accactatgg ttttgttgta gactccggaa
     6181 attaattcgg gagacatcct ctccaacacc cgagataatg aatcaattaa ttttatgatc
     6241 aggggttcag cagcatcagc taccaatttt gagtcaattt caaccttctg tggtttgttg
     6301 gtttccatgg actttccaat gacggtggtg tttagtggtt ccatttctgc attgcagtgc
     6361 accatcccca cttggatctt ggctttttct gcttcgtgta taccaatttc tactttgaag
     6421 agttctttca ccttttccac aatgttagta tcaacatcat ccccaccgct acggatggtt
     6481 tcgatgtctg tgattcctcc cagtgaaatc accactatgt cactggaacc tgcccctagg
     6541 tcaatcacca tggtgcctga ggcttctgct atgggtaatc cggcaccgat ggctgcagaa
     6601 agtccttcac taattaccag cacgtatttg gctccagctt ttcttccaat atcttccaca
     6661 gccttttttt ccacttcaga tgcatcacca ggaataccga ttacaattcg atcaatgctt
     6721 tccggacttt cacctgagcc ctgattcata gcataaacca ggagagattc cgcctgggca
     6781 atactttcga ttaccccttt tcgcagtgga cgaacggcta caatatcttc tggggtccgg
     6841 cctaacatcg ctttggcttc atctcccact gccagtacgt agcttggttc ctctttttta
     6901 acagctacta cagatggaat tttatataaa tcgaatttat ctcctgaagg catcgctact
     6961 acggtgttaa gagtccctaa atcaataccc aaagtatttg aaaggggttg cttcttaaca
     7021 tctacttttt catccttatt ttttaaaaaa tcgaacatat atttaatcct ccttcaaaat
     7081 tttgttaaag tctattataa ataaacggtt ttttttggcg attttcttga ttgtggaaat
     7141 ctgatcctct tgagcggata ttaggagagt ggacaatgcc acatctgcca tcttaaacaa
     7201 tggatcaatg gtatttctga ttagagtagg taacttatcg gtgatgtata catctgtttc
     7261 aatgaatccg gtggtctgat cttcgatgag gaaagatagg cgcatctgtt ctttttcaca
     7321 gtctcccaca aactttttta ttttattttc aggcccaact gttaaaaaga ggttaggttc
     7381 gttttcaacg taatatgggg ctatttttag ataagcacca ccattttctt tacaaatcaa
     7441 actcagatct cttattttat ttatatctcc gtagagcatg atgaaatcaa atttttttgt
     7501 aacaaaagat tcttttgtga ttacgtgttc tctatagagg atctccacac gatctttatc
     7561 cattatagca gcataagcta cattgttgac catttcctcg ttcccggcga tgacacacca
     7621 tatttcatcc gaatcttgaa tagtggaaac tttagccgct cttctgagga ttttaatttt
     7681 gttctctatc atttatgttt cacttcttag gatcagttat cccctgttgc tgttgcattt
     7741 ttctcgtgta atattcggtc tcaatcaaaa cagtcaagat tatttaattt acattaatat
     7801 aagtagtgtt tagttacaat aaattcattt tattttacgc agtgttccat ctaatttccc
     7861 aatatatacc ttgttataaa ttaaactttt cttaagaatt ccattagtag taatcacaac
     7921 atattcataa ataggataat ggacaccttg atatgaaaag aggataaaaa agttaagagt
     7981 tataaagctg gtgacgaaag aaaatgtttg ttaaagggac tataaaaaaa gcaggtattt
     8041 tgttatctct agcttttttg actttatttt tcggatatac ttacttggcc ttcatatttt
     8101 tcgcagccct tgtattcctt ttacaattct tccgggaccc ccaccgtgct gtaccggagg
     8161 gtaaagggtt aatactcgct tcagcagatg gaaggatttt taatgggaaa attgatacca
     8221 taaaaaccgt taattatgat gatcccctga tggaatatat tttagagaaa gagcaaaggg
     8281 gcatactggt aagtactttc atgtctgtct tcgatgtaca tgttaaccgt gttcccatat
     8341 ctgacagagt agtaaccacc aaatattatc caggaaaatt caaaatagca tctggaagtg
     8401 tacatactgt aaatgaaaag aatttaatag taatagattc agaatatggg aagttgggga
     8461 taattcaaat atcagggttc ttagctaggc gtattgtatc gtatgttggt cctggtgatt
     8521 gggttgagtc gggggatcga ttgggaatga taaaattcgg atcaagggta gatttaataa
     8581 ttcccacccc caattcagag gtcatggtta gtgagggcca aaaatcaaaa gcaggggaaa
     8641 ccatcattgc tagaatgttc tgatagatca acacgattta aaggcgtgtt aaaggggtgg
     8701 tgtgcattga ataaacataa aaatatgaat aataatttgt attataataa tattggagtt
     8761 tttttcaatg catattaaag attatttagc tgcaccagat ttactatcac ttttaaatgc
     8821 atcaactgga tttttatcaa ttttaatgct tttagatggt tattatatat tttcagccca
     8881 gttaatgctc ttagcagtga tatttgattc ccttgatgga tgggtggcta ggaagacaaa
     8941 acgcaatgat cattttggtt ttggaaaaaa cattgattca ctttctgatg taatttcatt
     9001 tggtgtagct ccgggaatgt ttttgtgtat tgcctctcca aatatgtttt taccatacat
     9061 taatatagta gtaggcttgt taattgttgt atgtggaata ttacgacttt ccaggttcaa
     9121 cgtactcatt attgacaacg atacgatttc caaggataaa ttcataggac tccccattcc
     9181 atctactgtt atggtactgg gatcctttta tctctctgga attttcagtg caaacatagc
     9241 atctttcctc atggtggtgg tagccgtgct tatgataagc accgttgagt atccaaaatt
     9301 taaaggtttg aagatcgtga tgataggtgc tttgttgatc attgcttcat gtttgcctca
     9361 gggaattatg tccttaattg cctatcttcc cgcaaagctt ttattagtct tcacattaat
     9421 atatgtatta atagtgcccc taatggaatt atacggcaaa cttctaagaa gtggtccaca
     9481 tgttagataa aatcttagga aaaaaagata aggataagaa agatgtcccc gatcttcgca
     9541 aggaagatac taaatctgac ccgaacattg gtggacgtat taagggcgtg gtatctaaag
     9601 ccacagataa gtctaaggag aagatttctc ccgatgataa cagccttaaa aagtcagaaa
     9661 gtaagaactc taagaaaaaa gcaatttcta agaaaatacc gaggcccatc ccaaaaccac
     9721 gtaaaaaatc agatgaaaaa cctaaattaa agcctccaat agagccaaaa aagaaaccta
     9781 gtggtttgag cagtggattt gggagaaaag ggcctgatga tgatcaaaga actctggttg
     9841 gtgccgcggt atttagtatt attttgatag ttttggtagg tgccggttat tactttttag
     9901 tgtatcaacc atatcaggaa actctgggtt cagctaagca aactaaatta aatgaagtta
     9961 acgcctattt taaaggtcct cttgctttag atcctcagaa acagactctg acagctgaaa
    10021 ttgatggtgc tgagactcca gagcaagctt tagccgttga tgtcatgact cctgctactg
    10081 ctgcctggag agaatatcaa agccaggaga taaatgccca gaaggatccc tacggaaggg
    10141 ttatgatcac ttactctgcc gccggccaga aaaatctgat aatgaaggtg gccgacgccc
    10201 aaaccattgt taatgaagct gatgccagtg tcctggccaa catggtcatt gaaactcccg
    10261 atacagtggc ggtgcccatt ataatcactc ggctacaagc tgcaggtgga ctggtaaacg
    10321 tgggtgacag tgtggatgtg tatctgcgaa ctactacccc caccaatgaa tccaaccaga
    10381 cagttaatcc agacatcaac aacacctccc ccagtatcag tggggcaact gttctggcca
    10441 ttctccgggc agtggacagt ggatctgtag atgctaattt aaagaatgca caggaagtcg
    10501 ccataaacca ggtgaccctg agtaattctc gaagctcctc tgtctccacc aatgttgaac
    10561 aacttctaag agcatctgca tctggtacat gggatagatc cacagtatca tctactctga
    10621 atgcttatgg ttggaagcta tcagatttcg aaagggcatc taatctggga gaactggatg
    10681 tgaaatatct ggtcttactg gaagtgcctc gtgaaaatgc tttgttcctg atgcagaatt
    10741 cggatagcat tgccttgact gtatctactc agaaagctcc gcagtggatg attcaggagt
    10801 taaaatcaat ttatggataa ttggtgaata aaggaaacaa catgttatta accaaaatga
    10861 ggagtttaaa catgataaaa aaagcgactt tggtggtagc tttccttttc actttttcca
    10921 tattcctggg ggcatcatgg gctgctaccg accagcaagc atgggttcag gaaactgact
    10981 ccgctctaaa attcacatac ttggaagctg ttatccatgt gaagattaaa aacaacaatg
    11041 atcatgtgga atattttaaa ataagccagc aatactacca aaccacccca gccatcaact
    11101 ggactattaa gtggaccaac ccagctgccg tgaagatggc caaatatatc aacactcccg
    11161 gtgattatgg atggaagata gaaccgggac agactaagga tgtatctttt aaacttgttg
    11221 ccttggctcc taatgggggt cctttgagtt tatatccggc ttacattgct cgcaatagtt
    11281 ctattcccaa ccaattctgg cccattctaa acgaaccggg tttaactgct acttggtttt
    11341 ttcccaacga aatcgagtat ttgaacccca atctggacct tgtttcatgg cgtggtaagt
    11401 tctgtttctg gataaagaac atggataaca ccggtcctag agtagaagga attgtaagag
    11461 cccccattgt accaatagac tctaaactcg tcttcagcaa tcctaaagtg acttacttgg
    11521 atgatgaact tccttgggcc gacactgctg cctgggatgt caccctgtat cctggacagc
    11581 agaaacatta ttcatacacc tactattggc ccgaaaatgt caccggccat tccactggta
    11641 aatataagtc cccatcagta tctactaaaa gtaatgctca ggataccgcc accactgtac
    11701 caactgctgg aactggtgtg ccctatgccc tttttgcggt aggggctatt atcgtgggat
    11761 ctggagtagt ctacgccaag ttattccgat aagttcttcc cacctgggga ggacttatat
    11821 ctccgacagt gacatattca gtgaacaaca tgaaa
//
LOCUS       DAOQVW010000003         9252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_8325_length_9252_cov_3.024791, whole genome shotgun sequence.
ACCESSION   DAOQVW010000003 DAOQVW010000000
VERSION     DAOQVW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9252
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8325_length_9252_cov_3.024791"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PKK85_00145"
     CDS             complement(<1..171)
                     /locus_tag="PKK85_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNS24466.1"
                     /translation="MAKTEKIIGIDLGTSNSAAAVLVAGKPTIIPSAEGATQYGKSFP
                     SYVAFTDDGQRLV"
     gene            complement(174..692)
                     /locus_tag="PKK85_00150"
     CDS             complement(174..692)
                     /locus_tag="PKK85_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNS24467.1"
                     /translation="MSKDQDIKNQKDDLNDLKDQIQEKDEIIQENEQRIDECKDKLLR
                     MQADLENFRKHTEKRMVEHVEQANQDLIMKILEIYEDLQRALEADKSEDLGEGVELIY
                     QKLRAVLENEGLQEIPAEGEKFDPFKHEAMMVESSDDYDNGCIIQELSKGYILNSKVI
                     KYSKVKVCKKGD"
     gene            879..1358
                     /locus_tag="PKK85_00155"
     CDS             879..1358
                     /locus_tag="PKK85_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNS24468.1"
                     /translation="MDLELILDVMGCKTRRNILDFLKDEPRFVSQISKELEVGQKAII
                     EHLRAMEEVGLLSSSYQKIERGRPRKYYDISQDVEFQIFISQGTIRIKVPGHEFQELQ
                     MLEERSLQGEDVSHDLKSLIENYDQAKKHAEDLLNDLEKRKRALKIRSMNLPVKYTP"
     gene            complement(1381..3678)
                     /gene="hypF"
                     /locus_tag="PKK85_00160"
     CDS             complement(1381..3678)
                     /gene="hypF"
                     /locus_tag="PKK85_00160"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNS24469.1"
                     /translation="MPKARILVQGIVQGVGFRPNIYRLAKDNHINGYVRNLGNIVEII
                     AEGEEKDISKFSEDIGIKKPPISKINSMELEWISVDDNQTYEDFQILKSSADFSGSSV
                     IPPDIAVCDSCLEEIFSISNRRYEYPFTACTDCGPRFTVINAVPYDRERTSMDKFPLC
                     PDCMDEYQTVEDRRYHAEASCCPVCGPQVFLHGVESKNPIKNAAELLDDGQILAIKGI
                     GGTHLVAKTSDDLPVARMRKRLGRSKQPFACMSPDVETIRTFADVEYLEEKTLLSRSR
                     PIVILKKNSNYYLSPLVSPALHNLGVMLPYSGLQHLLFKYGAEPAYIMTSANMPGEPM
                     LVKNQEIVEKLDGIADYFLLHNREIINRCDDSVVRFRGEDLAFIRRSRGYVPEPYDLS
                     SLNKDLNVLALGPEIDVTFSLLKEGRCFVSQHIGDTTKYDTFLYLQNAIKYMMEITRT
                     DGVDLVACDLHPMFFTTKLAHEIGERFHCPVVKVQHHHAHAAALGVDHGMDEMICIAA
                     DGVGYGDDGSIWGGEILHWQGKEYERMGSLMPQKMPSGDLTTKYPSRMLMAMVHEYYS
                     QEELVELMKSDYIDYFQHGKREVELVARQLERDFNITITTSTGRVLDAISAALKICGE
                     RTYEGECAMKLESAAIEGNYKLEIPLKIKKRRGLDILDTSPILMSVLEHKSEGESISD
                     IACSAQRVVAKGLAELAIRSADKVGVNVIGGSGGVFYNEAISMTIKEEIESAGYNFIQ
                     HQNSCAGDGSVSLGQVVVAVMKNLN"
     gene            3830..4609
                     /gene="larB"
                     /locus_tag="PKK85_00165"
     CDS             3830..4609
                     /gene="larB"
                     /locus_tag="PKK85_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNS24470.1"
                     /translation="MRDVLQQVLAGKISLKEAEKKLKSMQIKELGDFAKLDTARKMRT
                     GFPEAIYAEGKEEGELIQIIKSCSQNGRLLVTRLSLEQFEKMKSRLDELKPSGLDIEY
                     NPRARVLVVMGPGANDQENEKIGKIGLITAGTSDIPVAEEARVVAEVAGCQVISAYDV
                     GVAGIHRLFSQITTMIDEGVKAIIVVAGMEGALPSVVAGLVDVPVIGVPTSVGYGVGE
                     GGFAALYAMLQSCAPGIAVVNIDNGFGAAVFAATILRSSIK"
     gene            complement(4747..5436)
                     /locus_tag="PKK85_00170"
     CDS             complement(4747..5436)
                     /locus_tag="PKK85_00170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS24471.1"
                     /translation="MKVNDHHEILREKFGQGAKEYDQQRKHIIPCLDDLYHILADLTR
                     TNIQQPRILDLGAGTGLLTSYIYDRHPQGHFTLLDISEEMLNIARDRFKNSHDFTYVV
                     ADYTHHEFRESFDLIVSSLSIHHLKNQDKQILYQKIYDLLNPGGVFLNADQVLGPTPA
                     TEEEYQRNWMQKLDVGSLSEFDKNIILDRMQLDKPATLNENLTWLKNIGFIDVDIYYK
                     YYNFCILHGKK"
     gene            complement(5904..7709)
                     /locus_tag="PKK85_00175"
     CDS             complement(5904..7709)
                     /locus_tag="PKK85_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HNS24472.1"
                     /translation="METKKMVLLDIDYITENESAIIRLFGTFMTEKGPKSIIALDKNF
                     LPYIYVIPIHSERCREELAEFDVARVEQVARRDLGRTVQVLKVFFKHPRDVPGLRDEI
                     ANLSSVDEIREYDLPFYRRYLIDKGLFPMGKLEVHGQPVATGEYSGCPAEDTYLLEIS
                     EDPVPLESDFPELKILSFDIEAYNPKGMPRAEEDPIIMISFSSNEGLRKVISMAQCDF
                     DFVKTVATEEDILKEFVTTIKNENPHIILGYNSDNFDFSYINERAKLLGIPIDLGTDG
                     SSLKFMRRGFTNAALVKGRVHVDLYPVVRRYLSMDRYTLERVYWELFGQDKEDIPGDE
                     ICDYWDNGGLQLASLFKYSLDDAESVTKIGEKMLPLTMELTRIVGQPFFDVARMATGQ
                     LVEWYLIRKAFETRELVPNKPSAAEHSHRRGKRAAGGYVKDPEKGLHENIVYFDFRSL
                     YPSIIISKNVSLDTLVEECEEGACHVSPEAGYLFLKEPRGFVPSVIGNVLTERVRLKT
                     LMYQATDEREIQVLNVQQEALKRLANSMYGVYGFSRFRWYSLECADAITAWGRDYIKK
                     TMKKAEKFGFKPIYADTDGFYAVYDRKADSASDPS"
     gene            7837..8466
                     /locus_tag="PKK85_00180"
     CDS             7837..8466
                     /locus_tag="PKK85_00180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS24473.1"
                     /translation="MKFEKFDMQKHNLLKVAELIYEADTETFDFFFSEKEKSTQVIEK
                     LVLADVNTLNHQHIYVIVDGNNQVLGAAVIHHGKRPSFLDELKSIFKNMNLGDSLKYT
                     LIFILDSIFLSDLEDQDSYLAILAVDESVRSRGIGSFILKNAIHLMKEHGSKRMVLDV
                     DIENPGALRLYEKVGFRIFNKKSFSLLGWKKGVFNMEFISSGWCSGDLF"
     gene            8862..9242
                     /locus_tag="PKK85_00185"
     CDS             8862..9242
                     /locus_tag="PKK85_00185"
                     /inference="COORDINATES: protein motif:HMM:NF022953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HNS24474.1"
                     /translation="MRVITLIALLLVLFMGVTYGVFCATGEEPVDMKGQVVGVCYPNS
                     QNDTNSIKAVLVQGTMTDNNKTMNISVKISEETVIMEKIDGQRFNSSFENLQPGQTVE
                     IRFTGPFLQSYPPQTTARQIIILK"
BASE COUNT         2645 a         1974 c         1861 g         2772 t
ORIGIN      
        1 caccagtctt tgcccgtcat cagtaaaggc cacgtagctg ggaaaagact ttccatactg
       61 ggtggcacct tcggcactgg gtataatggt tggtttacca gccaccaaca ctgcagcggc
      121 ggagttactt gttcctagat ctataccgat tattttctca gttttagcca taatcaatca
      181 ccttttttgc aaactttaac ttttgagtat ttaattactt tagaattaag tatgtagccc
      241 tttgagagtt cttgaatgat gcagccattg tcataatcat cactagactc caccatcatg
      301 gcttcatgtt tgaaggggtc aaacttctct ccttcggcag gaatctcctg caacccctcg
      361 ttttccagaa cagcacgtag cttctggtag atcagttcca caccttcccc gagatcttct
      421 gacttatctg cctccagggc cctttgcaag tcctcataaa tttccagtat tttcatgatt
      481 aaatcctggt tggcctgttc cacatgttct accatacgtt tttcggtgtg tttccgaaaa
      541 ttttccaaat cagcctgcat tcttagcaac ttatccttac attcatcaat gcgctgctca
      601 ttttcctgaa ttatttcatc cttttcctga atctgatcct ttaaatcatt cagatcatcc
      661 ttctgattct ttatatcttg atctttactc attgcttcac ctttatgggg atggctaaga
      721 acagtaacct tctctattat agttaccaaa agttatataa acctatctct attatagtaa
      781 ccatacgtta tataaacctt tctctattat agtaaccata ggttaataaa attatcagat
      841 taaaaacatc atttaagatg agggttagtg gtattaaaat ggatttagaa cttatactgg
      901 atgtcatggg atgtaaaaca cggagaaata tattagattt tcttaaagat gagccaagat
      961 tcgtaagtca gatttctaaa gaacttgaag ttggacagaa agccataata gaacatttaa
     1021 gggctatgga agaagttggc cttttaagtt catcatatca gaaaattgaa cgtggaaggc
     1081 cgcggaaata ctacgatatt tcccaggatg tagaatttca aatatttata agccagggca
     1141 ccatccgtat aaaagtcccg gggcacgaat tccaggaact gcagatgctt gaggagcgat
     1201 ccctccaggg ggaggatgtt tcccatgatc taaaaagcct gatcgaaaac tatgatcagg
     1261 caaaaaaaca tgccgaagac cttttaaatg atttggaaaa aaggaaaagg gccctaaaaa
     1321 tacgctccat gaatctacct gtaaaataca ccccatagtt atgtctttat catgcatatc
     1381 tcagtttaga ttcttcatta ctgcaaccac aacttgaccc agggacaccg acccatcccc
     1441 ggcgcagcta ttttggtgtt ggatgaaatt ataccctgcc gattcgattt cttctttaat
     1501 ggtcatgctg atggcttcgt tataaaagac gcctcctgat ccaccaataa cgttaactcc
     1561 aactttatcc gcacttctta tagccaattc agccaggccc ttggccacaa ccctttgggc
     1621 agagcaggcg atatcagaaa tgctctcacc ttctgatttg tgttctaaaa ccgacataag
     1681 aatgggggag gtatctagaa tatccaatcc acgtcttttt ttaattttta atggtatttc
     1741 cagcttataa ttcccttcaa tggccgcaga ttccagtttc attgcacatt ctccctcgta
     1801 tgtcctttct ccacatatct tgagggctgc tgagatggca tccagtaccc ggccagtgct
     1861 ggtggtgatg gtgatgttga aatcgcgttc caactgacga gctactagct ccacctcacg
     1921 tttcccatgc tggaaataat caatgtaatc acttttcatg agttcaacaa gctcttcctg
     1981 ggaataatac tcatgaacca tggccatcaa catccgtgac ggatatttag tggttaaatc
     2041 gccactgggc attttttggg gcattaaact acccatccgt tcatattctt ttccctgcca
     2101 atgtaggatc tcccctcccc agatactgcc atcatcgcca taaccaacac catcggctgc
     2161 gatgcatatc atctcatcca tgccatgatc cacacccagc gcagcggcat gggcatgatg
     2221 atgctgcact ttaaccacag ggcaatggaa acgttcccca atttcatggg ccaatttcgt
     2281 ggtgaaaaac atgggatgaa ggtcgcaggc cactaaatcc acaccatcag ttcgggttat
     2341 ctccatcata tattttatgg cattctggag gtacaagaac gtgtcgtact tggtggtgtc
     2401 cccaatgtgc tgggaaacaa agcagcgtcc ctcctttaaa agggagaaag taacatcgat
     2461 ctcagggccc agtgccagga cgtttaaatc tttgtttaaa ctggataaat cgtaaggttc
     2521 aggtacataa ccccgggatc ttctgataaa agccaaatct tcacccctaa atctgaccac
     2581 cgagtcatca cagcggttga tgatttcacg gttgtgcagg agaaaataat cagcaatccc
     2641 atccagtttc tccacaatct cctggttttt aaccaacatg ggctccccgg gcatgtttgc
     2701 tgaagtcatt atgtaggctg gttctgcgcc gtacttgaaa agtaagtgct gcaaaccaga
     2761 ataggggagc attacaccta ggttgtggag tgcaggggaa accaggggag ataaatagta
     2821 gttactattt tttttcaaaa taactatggg cctactccta gatagcagtg ttttctcttc
     2881 caaatattcc acatcagcga aggtacgtat ggtttccaca tccggggaca tgcaagcgaa
     2941 gggctgtttt gaccggccca gtcttttccg catccttgcc actggaagat catcactggt
     3001 cttagcaacc agatgtgtac ctccgatacc cttaatagcc aatatctgcc catcatccag
     3061 caactccgcc gcatttttaa tgggattttt tgattctacg ccatgtaaga atacttgtgg
     3121 gccgcacact ggacaacagg atgcttcagc atggtaacga cgatcctcca ctgtctggta
     3181 ttcatccatg caatctgggc ataagggaaa cttatccata gaggttcgtt ccctatcgta
     3241 tggcaccgca tttataactg tgaaacgggg gccgcagtca gtgcaagcag tgaaggggta
     3301 ctcgtagcgc cggttggaaa tagaaaaaat ttcttccaga cagctatcac agaccgctat
     3361 atcaggaggg atcacagaag aaccagaaaa atcagcggaa ctttttagta tctggaaatc
     3421 ttcgtaggtc tggttatcat ctacagatat ccattctagt tccatggaat ttatcttgga
     3481 gataggtggt ttctttattc ctatgtcttc agaaaattta cttatatcct tttcttcccc
     3541 ctcagcgatg atctccacta tgttccctag attacggaca tatccattaa tgtggttatc
     3601 ttttgccaga cgatagatgt tagggcgaaa tcctacaccc tgaacaatac cctggaccaa
     3661 tattctagct tttggcatga ataactccat tttaattact tcaacaggta ataatcacta
     3721 aaaaaagtct gaatatgaat attcataaaa caactaccta agaattgttt ttacatccaa
     3781 ctttataaaa cctccacctt tatattatat gtgtgtatag gatgaaatta tgagagacgt
     3841 acttcaacag gttttagccg ggaaaatttc cctgaaagaa gctgaaaaaa agcttaaatc
     3901 catgcaaatt aaggaactgg gggattttgc caagttagac actgcccgaa agatgcgcac
     3961 cggtttccca gaggcgatat acgctgaagg aaaagaagaa ggggaactta ttcagataat
     4021 caaatcatgc tctcagaacg gccgtttatt ggttactagg ctcagtttag aacaattcga
     4081 aaagatgaaa tctcgtttag atgaattaaa accatcaggt ctggatattg aatacaaccc
     4141 ccgggccagg gtactggtgg taatgggccc gggtgccaat gaccaggaaa atgaaaaaat
     4201 tggaaagatc ggcctgatta cagctggcac atcagacata ccagtggcag aggaagcacg
     4261 ggtggtggct gaggtagctg gttgccaggt gataagtgcc tacgatgtgg gggtggctgg
     4321 catacaccgg ttgttttcac agataactac aatgattgat gaaggggtta aagccattat
     4381 agttgtggcg gggatggaag gtgcccttcc ttcagtggtg gctggcttgg tggatgttcc
     4441 agtaatagga gttccaacct ctgtaggata tggggtgggt gaaggaggat ttgcagcttt
     4501 atacgccatg ttacaatctt gtgccccagg aattgcggtg gtgaatattg ataatggatt
     4561 tggggcagca gtttttgcag caaccatact ccgatcctcg ataaaataga aacttatcct
     4621 atttatttat gttgattctt ttctccagat ccccatgggg acgtgtctat ctgcaccacc
     4681 aacttttata aaatccaata aaacatcatt ccgggccatc aagtattcct acttttttat
     4741 agttccctat ttcttcccat gcaggatgca gaagttgtaa tacttatagt aaatatccac
     4801 atctataaaa ccaatgtttt ttaaccatgt gaggttttcg tttaaagtgg ctggtttgtc
     4861 cagttgcata cgatccaaaa tgatattttt gtcgaattct gagagtgacc ctacatctag
     4921 tttttgcatc cagtttcttt gatattcctc ttcagtggcg ggggtgggtc ccagtacctg
     4981 gtcagcattt aaaaaaacac cccccggatt cagcaagtcg tagattttct ggtacaaaat
     5041 ctgcttatcc tgatttttca gatgatgaat ggagagtgat gaaactatca ggtcgaatga
     5101 ttcccggaat tcatggtggg tgtagtcagc caccacataa gtaaaatcgt ggctattttt
     5161 aaaccgatcc cgagcgatat taagcatctc ctcggagata tccaagaggg tgaaatggcc
     5221 ctgaggatgt ctatcataaa tatacgaagt taaaagccca gttccagccc ctaaatctag
     5281 tatccggggc tgttgaatat tggtccgggt caggtcggct aatatgtggt ataagtcgtc
     5341 aagacatggg ataatgtgct ttcgctgctg gtcgtactcc ttggcgccct ggccaaattt
     5401 ttctctcaat atttcatgat gatcatttac cttcacagta aattctccat tccttttaac
     5461 ttacagttca tcaggtatct gttctagaat cactgttgag gccaatataa ggccggtaac
     5521 atgaatggca tcctgggtac gtggatactc ccaaagtact ccttttcagt ttgacacttg
     5581 aaaggatatt aagcgacatc ctcgattgat aatggtcatg gtttaaacca cattttccac
     5641 cagttccccc aatatggaag gtttacttga ttgtagtcct ggttcggaca tgtttaggtt
     5701 ttttcccagg atatctccaa ataccttcct tgtcctgttt tttctgtatc tttttaacag
     5761 tttaagatgt tcacactatt ctaacaatac actacaggta gatgctcatg aatcgattac
     5821 ccatgaaagg gctttaatag gatcagtgtt tagttcatct agccagccat aatgggttga
     5881 ctccaacatg aataacatct cagttaggat ggatcacttg cagagtcagc ttttctatca
     5941 taaacagcat agaaaccatc agtatcagca tatattggct tgaagccaaa tttctccgct
     6001 tttttcatgg ttttctttat ataatcccgg ccccaagcgg taatagcatc ggcacattcc
     6061 agactatacc atctaaacct ggaaaacccg taaactccat acatagagtt agctaaacgt
     6121 ttcagtgctt cttgctggac gttcaacact tggatttctc gttcatccgt ggcctgatac
     6181 atcagggtct tcaaacgcac cctttcagtt agaacattac caatcaccga aggaacaaat
     6241 cctcttggtt cttttaaaaa caaatatcct gcttcagggg acacatggca ggctccttct
     6301 tcacattctt ccaccagcgt atccagggac acgttcttgg atatgatgat actggggtag
     6361 aggcttctga agtcaaaata cactatgttt tcatggagtc ccttctcagg atccttaacg
     6421 tatccaccag cagcacgttt ccccctacga tgggaatgtt ccgctgcgga tggcttgttt
     6481 ggaactaatt ccctggtttc aaatgccttt ctgatcagat accattcaac aagttgtccc
     6541 gtggccatgc gggccacatc aaaaaatggt tggccaacga tcctggttaa ctccatagtt
     6601 aagggcagca tcttctctcc aatcttggta acggattcag catcatctag ggagtatttg
     6661 aagagtgaag ccagttgtaa tccgccgtta tcccagtaat cacagatttc atctccgggt
     6721 atgtcttctt tgtcctggcc aaaaagttcc cagtaaaccc tttccagggt gtaacgatcc
     6781 atggacaagt agcgacgcac cactggatac aaatctacat gtacccggcc ttttaccagt
     6841 gcagcgttgg taaaacccct tctcatgaat ttaagggagg atccatcagt gcccaagtct
     6901 atgggaattc ccaggagttt agctctttca tttatatagc tgaaatcgaa gttatcggag
     6961 ttgtaaccca agatgatgtg ggggttctca tttttaatgg tagtgacaaa ttcctttaaa
     7021 atatcttctt cagtggccac ggttttaaca aagtcaaaat cacattgtgc catagaaata
     7081 acttttctta agccttcgtt actggaaaag cttatcatta ttatgggatc ttcttctgcc
     7141 cggggcattc ccttggggtt gtaagcttcg atatcaaagc ttaaaatttt tagttcagga
     7201 aaatctgact ctaaaggaac cggatcttca cttatctcca ggaggtaggt gtcttcagct
     7261 ggacatcccg aatattcacc ggtggcaaca ggttgaccat gaacctccaa ttttcccatg
     7321 ggaaaaagtc ccttatcaat caggtatctg cgatagaatg gtaaatcgta ttctctgatt
     7381 tcatcaacgc tacttaaatt agcaatttcg tctctcaagc cgggaacatc cctgggatgt
     7441 ttaaaaaaaa cctttaaaac ttgcactgtt ctgcccagat ccctgcgggc cacttgttct
     7501 accctagcta catcgaattc agccaactcc tctcggcacc tttcagaatg aatgggaatt
     7561 acatagatat agggtagaaa gtttttatcc agtgctatta tagatttagg tcctttttca
     7621 gtcatgaatg tgccaaataa ccttattatg gcactttcat tttcggtaat gtagtcaatg
     7681 tcaagtaaga ccattttttt ggtttccatt gctcccaatt tatacgaagg ctggtataat
     7741 attttcttag attcatacga attatccaaa acgatcgtac tttgcaagtg taaagatagc
     7801 gattaataac aggttaagat caaacaagtc tccagaatga agttcgaaaa atttgatatg
     7861 cagaaacaca accttcttaa ggttgctgaa ttgatatacg aagctgatac agaaactttt
     7921 gatttctttt ttagtgaaaa ggaaaaaagt acccaggtaa tagaaaaatt ggttcttgcc
     7981 gacgttaaca ccttgaacca tcagcacatc tatgtgattg ttgatggaaa caaccaggtg
     8041 ttgggtgcag cggtcatcca ccatggaaaa agaccttctt tccttgacga gctgaaatct
     8101 attttcaaaa acatgaatct tggtgatagt ttgaagtaca ccctgatctt tatcctggac
     8161 agtattttcc tttcagatct tgaagatcag gacagttatc tggccatatt ggcagtggat
     8221 gagtcagtca ggagcagagg aattggttca ttcatcctga aaaatgcgat tcacctgatg
     8281 aaagaacatg gtagtaaaag gatggttctg gacgttgata tagaaaaccc cggcgcccta
     8341 cgattatacg aaaaagttgg tttcaggatt tttaataaga aaagtttttc gttattggga
     8401 tggaaaaagg gggtttttaa tatggagttc atttccagtg gttggtgtag tggggacctg
     8461 ttctgacaca tccagttaca ttggaaacag tggagactta ttattaaaat ttagaatctt
     8521 ttacaaagat atacagttaa aattagctat gctgataaat tgagtgtttg aacatgtttc
     8581 attaacttat cctgcttaaa agacttaata ggtcagtgta cttcaatttc caaaacttca
     8641 aagaatatat ttggaacgat tgattaaaat gcttcagact attttttaaa gaatattatg
     8701 gataaatagt ttttgtttac aattccagga agaatattta ttcttagtaa atttaatcct
     8761 ctcagtcaag ccttaaatgg aatacatctt tttagtaggg gagttgggat gcaaaaacat
     8821 attaataatg gtacaacata ttaaccgtga taggagagaa catgagggtc ataacattaa
     8881 tcgcattgct gctggtattg tttatgggtg taacctacgg agttttctgt gctactggtg
     8941 aggaaccagt agacatgaag ggccaagtgg tgggagtgtg ttaccctaac tctcaaaacg
     9001 atactaacag cattaaagcg gttctagttc agggcacaat gactgataac aataaaacca
     9061 tgaatatctc cgttaaaata tctgaagaaa ctgtgattat ggaaaaaatt gatgggcaac
     9121 gctttaacag ctcttttgaa aatcttcaac ccggtcaaac cgtagaaatc agatttaccg
     9181 ggccattcct acagtcttat ccgccacaaa ccactgcccg gcagattatc attttaaaat
     9241 aagaatttct at
//
LOCUS       DAOQVW010000004         8073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_9869_length_8073_cov_3.531928, whole genome shotgun sequence.
ACCESSION   DAOQVW010000004 DAOQVW010000000
VERSION     DAOQVW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9869_length_8073_cov_3.531928"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKK85_00190"
     CDS             complement(<1..402)
                     /locus_tag="PKK85_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNS24475.1"
                     /translation="MLHGTKILKEDFAKMTRGGVVMDVVNADQAVIAEEAGAVSVMAL
                     ERVPADIRATGGVARMADPLKVEEIMEAVSIPVMAKIRIGHFVEAQVLEALGVDMIDE
                     SEVLTPADEKYHVDKKKFTIPFVCGARNLGEA"
     gene            1038..1226
                     /locus_tag="PKK85_00195"
     CDS             1038..1226
                     /locus_tag="PKK85_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24476.1"
                     /translation="MFDIIFYIRLIAGVCFLISGIAFLKRKKEEKRDISCLLFILCIL
                     GGVLQFIGALAYLLDKTG"
     gene            1516..3345
                     /locus_tag="PKK85_00200"
     CDS             1516..3345
                     /locus_tag="PKK85_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNS24477.1"
                     /translation="MKKRVGAILLVMFVVLVLGVNSVSAVNVTPDQVADASENVNSYI
                     NASHTIPGNTNIGGNQVDMPQYLKLSTEAVLNINDGSTASIPIGSYGTPISPSETITS
                     RNFSKAEYLELTTRVKNFMDANGRAPNYASTSTGNIRYESLIYMYSGILNSYRMNGVL
                     PDYITVNPWTTVLSSDVIGSTSYGYVEKKFFGNQSSPQTIALIIGVHPLENGIHNAIL
                     NTLTSKSLDLTKRYVLYQVTVTKDANDYNNGRMNGQLLAQAFIVPDIAKENPFLVVDN
                     HENRGANSGYQYYRFLYLISNNADTTNYANQIIRQMPSLAIYSPPNPTSPSYVTIPIA
                     KKGICTMIYETYLYDSVSKKTSDANALINALEGVNTPYIAPLKVKITGDPYTGWYISL
                     TINTPGSFTIYYTTDGSDPELSTTRTQYTSSQSVHINNLLRISMVDVYGNWYSVFNES
                     PLVTTATTGGTYYTPQNVVLEAINGSYGNIVIYHTTDGSDPRSSSTRKQYVAPINVSS
                     SQTLYYYAVDGAGHQSPVCMEQYHIYKTVSYSYTVDVPYKKGWYKYWYKVSYKKWYKS
                     WYKYRGKWRYKWQYKWAVKSKYKWKYGWIYRKETRWGTKNVLT"
     gene            complement(3342..4499)
                     /locus_tag="PKK85_00205"
     CDS             complement(3342..4499)
                     /locus_tag="PKK85_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HNS24478.1"
                     /translation="MVAKIGTAHLPLHGGRAPWWLFKRMVKLSGNITEALVYEYGASE
                     FLRRISDPHWFQALSCVLGFDWHSSGTTTTTCGALKSSLNPEDHGILVAGGKGRASRR
                     TPEEIEAAGELFSLSTSSIEDLVHYSRMVAKVDNTCIQDGYQLYHHVFFLTEGGEWAV
                     VQQGMNQEYSYARRYHWLSSKVEDLVENPHQGICCDSREEEALDMTAPESKEARQISV
                     ELVQDNPEHLKKYFQSKPRLEVSQSSLHDYCPELNLPAHHPVLNTDLTDRDLEVLKRA
                     WEIQPSNYEELLGLEGMGPKKIRALALISDLVYGSQPSWEDPVKYTFTHGGKDGFPYP
                     VDREVYDHSISTLQDALEQARLDKKDRYQALKRLEGLVSGEQKKIKKNTNV"
     gene            complement(4565..6328)
                     /locus_tag="PKK85_00210"
     CDS             complement(4565..6328)
                     /locus_tag="PKK85_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNS24479.1"
                     /translation="MKLSIIIPTYNEENYLPLLLKSIQAQNFSDYEIIIADAGSQDRT
                     REIARDADCRVVDGGLPAVGRNRGAESAQGEYLLFLDADVCLTDGYLKTSLEEFAAEK
                     LGIAITQISPLSESRLDKMAHDFANFFMRRVESFKPHGAGCYGILTLKKLHEEVEGFD
                     EDLDFGEDTDYIERVAEISSFKVLRQPRLLVSTRRVEEEGMKRVAYKYAKSTLYQFTG
                     RKITAEKLNYKFGHSENTRILYSVCGEGMGHAIRTDVILKHLTPEHDVHIFSSDRAYQ
                     YLADRYDNVYEIGGFNTVYEDNRVKNTKTFIQGMKGLPSDLRNSLRLMYSVAKSVKPH
                     IIISDFEIYGNLLAKILRLPLISLDNMHIITQTEIEVSSQYRTERLAAEGVIRSFINM
                     PRCYLINTYFYPPIKNPQKAKLFPPVLREEIMKLKSEEGQHILVYQTSDSNLKLLDLL
                     KELDEEFIIYGFHKDEQDTNLTFKSFNDKEFLQDLATCRAVLTNGGFTLISEALYLKK
                     PVLSVPVKKQFEQILNAIYLDRLGYGEYHEDLEKGDVLKFLKKLEIYRDNIEKNFQHD
                     RNQGITEELDELIQKYGPTRK"
     gene            complement(6356..7378)
                     /locus_tag="PKK85_00215"
     CDS             complement(6356..7378)
                     /locus_tag="PKK85_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24480.1"
                     /translation="MLKIEYQDLIGIILILLGVLVYVTDFLDGVMMTITWPLLSGSSE
                     GKAILLLVTMGSLLLINSLIRSRRSFLDTIHSTLYNCSHEGREYLKWFIIIVLLTYAA
                     GIILEIWLRYRYGVSILTIFVSLNPSPTSTSPTHSHVFKAVLGSVIDWVGVNVPGNIH
                     TGGSLIQQVTPGAFLIIFTLPAAYILGLLSLDDRLVIHRLIMAFCMTLAMVGMLDGGL
                     FSQPGVMGLGGTLGMYAIKSPFQHRQLLYPALVVMLLIVSGVAVEVVGSNPAYHELTI
                     INPQASLDLSDYNVISIQEVENRTIVRIKTDQSDKEFIEGLFVSLDGKAQGFFMTWDF
                     ATYLGH"
     gene            complement(7398..>8073)
                     /gene="nadA"
                     /locus_tag="PKK85_00220"
     CDS             complement(7398..>8073)
                     /gene="nadA"
                     /locus_tag="PKK85_00220"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HNS24481.1"
                     /translation="ISAECPMAHMLPAKEVREYKRRYPDAAVVLYVNTLAEAKAEADI
                     LCTSANAVEAVESLSEKRILFGPDMNLASYVAQQVDKEIIPIPEGGHCYVHKMFQPTD
                     IMVMKEKYPGAELLIHPECDQELQEMADQILSTGGMVHYVASSPSDTFLIGTEMDMVT
                     RLSREYPDKTIVPLLSEAICTNMKLHTLEKVKNSLITEKYQVVVDPEVANRARSAVER
                     MLALPP"
BASE COUNT         2044 a         1930 c         1891 g         2208 t
ORIGIN      
        1 agcttcgccc aggttccggg cgccgcagac gaagggtatg gtgaacttct tcttatccac
       61 atggtacttc tcatctgctg gggttaaaac ttcgctttcg tcgatcatgt ccactcccag
      121 ggcctccagg acctgggcct ccacgaagtg gccgattcgt atcttggcca tcactgggat
      181 ggacacggcc tccatgatct cctccacctt caatgggtca gccatccggg ccacaccacc
      241 ggtggcgcgt atatcagccg gcaccctttc cagggccata accgaaacag cccctgcttc
      301 ctcggctatc actgcctgat cagcgttgac cacatccata accactccgc ccctggtcat
      361 cttggcgaag tcttctttta agattttagt tccgtgtagc attagatctt cctcctttga
      421 tcatcctaaa aatcagtata tttactgcaa ttcattttag aatattttca tgtactatat
      481 acagtctagg tttttgtggt ttactcttag tatattttct ttcttatttt ttttatccaa
      541 atttataaaa aattaaaaaa tttccaataa aaaaaagaat agatccttga aatattatgc
      601 aatagtgatt ttcccaataa tcaacggaca agcacccgcc aacgccaccc tactcaccga
      661 tgacgagatg cacaacatca aagccctctg acacatcgcc aaaatagaac acagctactg
      721 gataccatgt tactataata attaagtgta aaaacaatat taggcagctt agcttttgga
      781 gcgcccgggt ctttatgcta ctggtgctgt tgaaatttta actgttacgg gtataggtag
      841 tgcaagtgct ttcagatata gtgtagtggt cacaatagct aatgcaatga gtggggagct
      901 acagaaagca tttgaagatc cttactatga agtgaaagtt gaaggaattc ctttagtcca
      961 tatctgaata gtacacaata agttttgcca tgctatcaaa tactagagat agagaaaacg
     1021 tataaggggt aatatgcgtg tttgacataa ttttttatat tcgcttaatc gcaggtgtgt
     1081 gttttttaat ttctggtatt gcctttctaa aaaggaaaaa agaagaaaaa agagacattt
     1141 catgcttact ttttattttg tgtattttag gaggagtttt acaatttatt ggtgctttag
     1201 catacttgtt agataaaaca ggataggatt agggattatt atttctaata aatatatgta
     1261 cagctaagga ggttttttaa ccctgctcat gctcttactt tcggagtttt tacaccctta
     1321 tttatgatta tccgctgttt taaacccaat atcctccaaa aaaatccaaa aaaaacatac
     1381 aatgtaataa taacttatgc aaatgtaatc ataagttatt tatatcagga attattctta
     1441 agcaaattag gtaatttgtt cgaattcttt atagttaatt aggataaaga tattatatca
     1501 tccaggtggt aagttatgaa gaaaagagtt ggggccatat tgttagtcat gtttgtagtg
     1561 ctggttttgg gtgttaacag tgtttcggca gtgaatgtta caccagacca ggtagcagat
     1621 gcttccgaaa atgtgaattc ttatataaac gcctcccaca ccattcccgg taacaccaat
     1681 atagggggca accaggtgga catgccccaa tatttgaagc tttccaccga agcagtgctg
     1741 aatattaatg acggctccac tgcatccatc cccatcggaa gttacggtac tcccatttca
     1801 ccttcagaga ccattaccag ccgaaatttc agtaaagccg agtatttgga actgaccact
     1861 cgtgttaaga attttatgga cgccaatgga cgggcaccta actacgcatc caccagcacg
     1921 gggaatatcc gttatgaatc cctaatctac atgtactcgg gaatattgaa ctcctaccga
     1981 atgaatggtg ttttacctga ttacatcacg gttaatccct ggaccacggt gttaagctct
     2041 gatgtcatcg gcagcaccag ctacggttac gtggaaaaaa aatttttcgg gaaccagagt
     2101 tctccccaga ccattgccct aataattggg gtgcatcccc tggaaaatgg gatacacaac
     2161 gctattctta ataccctgac cagtaaatcc ctggatctga ctaaaagata tgttctctac
     2221 caagttacag tgactaagga tgccaatgac tacaataatg gcaggatgaa tggacagctc
     2281 ttagcacagg cattcatagt cccggatatc gccaaggaaa acccattcct ggtggtggat
     2341 aaccatgaaa accggggcgc caacagcgga taccaatact accggttttt atatttaatt
     2401 tcaaataacg ccgacaccac aaactacgct aaccagataa tcagacagat gccctcttta
     2461 gccatttaca gtccgcctaa ccctaccagc ccctcatacg tcaccatccc cattgccaag
     2521 aagggaatct gtaccatgat atatgaaacc tacctctatg actcggtttc taaaaaaaca
     2581 tccgatgcaa atgcccttat aaatgcctta gagggtgtta acacacccta tatcgcccca
     2641 ttaaaggtga aaatcacagg agatccttac actggttggt acattagttt aaccatcaac
     2701 accccaggtt ctttcaccat ttactacacc accgatggct ctgatcctga actcagcacc
     2761 accagaaccc aatacacaag ttcccaatca gtccacatca acaacctcct tagaatatcc
     2821 atggttgatg tctatggtaa ctggtattca gttttcaatg aatcacccct agtaacaact
     2881 gctacgactg ggggaaccta ttatacaccc cagaatgtag ttttagaagc aataaatggc
     2941 tcttatggta atatcgtcat ttaccacact acagatgggt ctgacccccg gagcagcagc
     3001 acccgcaaac agtacgtggc acccatcaat gtaagctcct cccagacttt atactactat
     3061 gccgtggatg gagccgggca tcaatcacca gtgtgtatgg aacagtacca tatttacaag
     3121 acggttagtt acagctatac tgtggatgtg ccttacaaga agggatggta caagtactgg
     3181 tacaaggtat cctacaagaa atggtacaaa tcatggtaca agtaccgggg taaatggaga
     3241 tacaaatggc agtataagtg ggctgtaaaa tctaaatata aatggaaata tggctggatt
     3301 tatcgtaaag aaacacgttg gggaactaaa aatgtgttaa cctaaacatt tgtatttttt
     3361 ttaatttttt tttgttcccc actgaccagc ccctccagcc tcttcagggc ctggtatcgg
     3421 tccttcttat ccagacgggc ctgttccaag gcatcctgca gggtgctgat ggagtggtcg
     3481 tagacctccc ggtccactgg gtaagggaaa ccatcctttc ctccgtgggt gaatgtgtac
     3541 ttgaccggat cctcccagct gggctgggaa ccatagacga ggtcggatat cagtgccagg
     3601 gcccggatct tcttgggacc cataccctcc aggcccagta actcctcgta attggagggc
     3661 tgtatttccc aggcccgttt aagaacctcc aggtctcgat ctgtcagatc agtattcaag
     3721 acagggtgat gggctggtag gttcaactct gggcaatagt catggagact gctctgtgaa
     3781 acttccaatc tgggcttact ctggaagtac tttttgaggt gttccgggtt gtcctggacc
     3841 agttccacgc tgatctgccg ggcctctttg ctttctgggg cggtcatgtc cagggcctcc
     3901 tcttcccggg agtcacagca aatgccctgg tgggggtttt ccaccagatc ctcaaccttg
     3961 ctggagagcc agtggtaacg tcgggcgtag ctgtattcct ggttcatgcc ctgctgcacc
     4021 accgcccact cccccccctc ggttaagaag aacacgtggt ggtagagttg gtagccgtcc
     4081 tggatgcagg tgttatcaac cttggccacc atccggctgt agtgcaccag gtcttctatt
     4141 gacgaggttg ataatgaaaa caattcaccg gctgcttcga tctcttcagg agttcggcgg
     4201 gaagcccggc ctttgccacc tgccaccagg ataccatgat cctccgggtt aagggatgat
     4261 ttcagggccc cgcaggtagt ggtggtggtg ccggagctgt gccagtcaaa gcccaagaca
     4321 cagcttaagg cttggaacca gtggggatcc gatattcgcc ttaaaaattc ggaagccccg
     4381 tactcgtaga caagtgcctc ggtgatattt cctgacagtt ttaccattct cttaaagagc
     4441 caccatgggg cccggcctcc atgtaggggc aggtgggcag ttcctatttt agcaaccata
     4501 ttacctagtt ctactttaga agggtaatgg ctttggtgag agcactggag aagtaatgag
     4561 cagattattt tcttgtgggc ccatatttct ggataagttc atccagttcc tcggttatgc
     4621 cctggttacg gtcgtgttgg aagttcttct caatgttgtc ccggtagatc tccagttttt
     4681 ttaagaactt aagaacatcc cctttttcca ggtcctcgtg gtactcaccg tagcccagcc
     4741 ggtccaggta gatggcgttg agtatctgtt cgaactgctt cttcaccggc acactcaaca
     4801 ccggcttttt taggtacagg gcctcgctga tgagggtgaa tcccccgttg gttaagaccg
     4861 cccggcaggt ggccagatcc tggaggaact ccttatcgtt gaaggatttg aaggttaagt
     4921 tggtgtcctg ttcatccttg tggaagccgt atatgatgaa ctcctcatcc aactccttta
     4981 aaaggtccag tagcttcagg ttggagtcgc tggtctgata aaccaggata tgctggcctt
     5041 cctcggattt cagcttcatg atctcctccc tgagaacagg tgggaagagt ttggccttct
     5101 gggggttctt gatgggtggg tagaagtagg tgttgatgag gtagcagcgg ggcatgttga
     5161 tgaaggatcg gatcacaccc tcggctgcta aacgctcggt acggtactgg gatgagacct
     5221 cgatctcggt ttgggtgatg atgtgcatgt tgtccaggct gatgaggggc aggcgcagga
     5281 tcttggccag gaggttgccg tagatctcga agtcagagat gatgatgtgg ggtttcacgg
     5341 acttggccac actgtacatc aagcggaggc tgtttctaag gtcgctgggc aggcccttca
     5401 ttccctggat gaaggtttta gtgtttttaa ccctgttatc ctcgtagaca gtgttgaaac
     5461 caccgatctc gtacacgtta tcatacctat ctgccaggta ctggtaggcc cggtccgagg
     5521 agaagatgtg gacgtcgtgc tccggggtga ggtgcttcag gatcacgtcg gtgcggatgg
     5581 catggcccat gccctctcca cagaccgagt acaggatcct ggtattttca ctgtggccaa
     5641 atttgtagtt cagtttctcg gctgttattt ttcggccagt gaactggtag agggtgcttt
     5701 tggcgtactt gtaagccacc ctcttcatgc cctcctcctc cacccggcgg gtggaaacca
     5761 gtagccgggg ttgcctgaga actttgaagg aggatatctc cgccactctt tcgatgtagt
     5821 cggtgtcttc cccgaagtcc agatcctcat caaagccctc cacctcctca tggagttttt
     5881 tcagggttaa tatgccgtag caccctgctc cgtggggttt gaatgactct accctgcgca
     5941 tgaagaagtt ggcaaagtcg tgggccatct tatccagacg gctttcactt aagggggata
     6001 tctgggtgat ggcaatgccc agcttttctg cggcgaactc ctccagtgaa gttttcaggt
     6061 aaccatcggt aagacataca tccgcatcca gaaataacag atattccccc tgagcagatt
     6121 ctgcaccacg atttcgaccc acagccggta atcccccatc cactaccctg caatcagcat
     6181 cccgggcgat ttctctggtt cggtcctggg atccggcgtc ggcgatgatt atctcataat
     6241 cactgaaatt ctgggcttgt atgctcttca acagtagtgg gaggtagttc tcctcgttgt
     6301 aggtggggat gatgatggac agcttcattt agacacctat aaatcttttt tttatctaat
     6361 ggcccaagta ggtggcgaag tcccaggtca tgaaaaagcc ctgggccttc ccatccaatg
     6421 agacgaataa accttctata aactctttat cactttgatc agtctttatc cggactatgg
     6481 ttcgattttc aacctcctgg atggatataa cattgtagtc agatagatca agggacgcct
     6541 ggggattgat gatggtcaac tcatggtaag ccgggttact acccaccacc tccactgcca
     6601 ccccagatac gataagtagc atcactacca gggccgggta gagtagctgg cgatgttgga
     6661 agggactttt aatggcgtac attcccaggg tcccccccaa tcccataacc cctggctggg
     6721 agaacagccc tccatccaac atgcccacca tggccagggt catgcagaaa gccattatga
     6781 ggcggtgtat gaccagacga tcatccaggg agagtaaacc caggatgtag gccgctggca
     6841 gggtgaaaat tatgaggaat gccccggggg tgacctgctg gatgagtgat cctcctgtgt
     6901 gtatatttcc agggacgttg acaccaaccc agtcaattac gcttccaaga accgctttga
     6961 acacgtggct atgggtgggg ctggtgctgg tggggctggg attcagggaa acaaagatgg
     7021 tgaggatgga aaccccgtag cgatatctca gccagatttc caggattata cccgcagcgt
     7081 aggttaaaag aactattatt atgaaccatt taaggtattc acgaccctca tgactgcagt
     7141 tgtaaagtgt ggagtggatg gtgtctaaaa agcttctccg ggatcttata agggagttta
     7201 tcaggagcaa acttcccatg gtcactagta agaggatggc cttaccctct gaggatccag
     7261 atagcagggg ccaggtgatg gtcatcatca caccatccag aaaatcggtt acataaacca
     7321 ggacacccag gaggattaag atgattccta tcagatcctg atactctatt ttcaacactt
     7381 cactacctat aattttatta aggggggagg gctaacattc gctccactgc actacgggcc
     7441 cggttagcca cctccgggtc caccaccact tggtattttt cggttataag agagtttttc
     7501 accttttcca gagtatggag cttcatgttc gtacatatag cctcggatag aagtggtact
     7561 atggttttgt caggatattc cctggagagc cgggttacca tgtccatttc agtgcctatg
     7621 aggaaggtgt cacttggtga tgaggccacg tagtgtacca tgcccccggt gctaaggatc
     7681 tggtccgcca tctcctgcaa ctcctgatca cattctggat ggattaacag ttctgcgccg
     7741 gggtatttct ccttcataac catgatatcc gtgggctgga acattttgtg cacgtaacag
     7801 tgaccacctt ctggtatggg aattatctct ttatccactt gttgggctac gtaggaggcc
     7861 agattcatgt cagggccaaa tagtattctc ttttcagaga ggctttctac tgcctccaca
     7921 gcattggccg aggtgcacag gatatcggct tcagctttgg cttctgccag tgtgttcaca
     7981 tataaaacca ctgctgcatc agggtaacgc cttttatatt cccggacctc cttagctggg
     8041 agcatatggg ccatggggca ctccgctgag atg
//
LOCUS       DAOQVW010000005         7907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_10122_length_7907_cov_3.263882, whole genome shotgun sequence.
ACCESSION   DAOQVW010000005 DAOQVW010000000
VERSION     DAOQVW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10122_length_7907_cov_3.263882"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKK85_00225"
     CDS             <1..499
                     /locus_tag="PKK85_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNS24482.1"
                     /translation="RNLGEALRRIDEGAAMIRTKGEAGTGNVVEAVRHMRMIQGTIRD
                     LKDKTEEELWGVAREIEAPLELVKETQKQGRLPVVNFAAGGVATPADAALMMQLGADG
                     VFVGSGIFKSEKPELVAQAIVEATTHYNNAEVLANVSRGLGKAMPGLEMSSIPAEERL
                     QDRGW"
     gene            complement(784..1125)
                     /locus_tag="PKK85_00230"
     CDS             complement(784..1125)
                     /locus_tag="PKK85_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNS24483.1"
                     /translation="MIKKAMIGLIILTILVVAVSGCLDTGNKNNTTNNNSNLNQNTTS
                     HTNITATQAQELAKKYIEQEGASAGTPQLTSINGKPVYIVPIIYKGKAVGQIEIDATT
                     GQNIGGAGGAP"
     gene            complement(1245..2024)
                     /locus_tag="PKK85_00235"
     CDS             complement(1245..2024)
                     /locus_tag="PKK85_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNS24484.1"
                     /translation="MPELPSVEIFKRYLDQHALNQMIKKVEVVSPELVVYADPRRFEG
                     SLEGHEFISSRRHGKYLFAQLDENQYLVMHFGMTGYLHYFHHNRSKYVRMLISFHNGF
                     HLAFDDARKFGKLGLTPDPDDFIAGKGLGEDALDISFKAFYKTIKDRRGLIKPLLLNQ
                     NFVAGIGNLYSDEILYQCGLHPETPAHQLSKSDVGDLYYQMRRVLEKAIECQDSPRDF
                     PDSYLLPHRYPGGRCPEGDPLEVIKVGGRTTYYCPEKQPLK"
     gene            complement(2137..3636)
                     /locus_tag="PKK85_00240"
     CDS             complement(2137..3636)
                     /locus_tag="PKK85_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNS24485.1"
                     /translation="MPFKVERNHELCRRNFDRPGCCWYMCDDRDENLCRNCYSCYNNC
                     PHEVYEIIDDEPYPIHHEQCVGCRICEEMCPNNAIEVNSVPEDRRNVWSLTDLAEINR
                     KATEGSYKVRGCGATRVIPTFDDLVIVPAQASRPPIDKYREPCNTRVVLGDRYAENPL
                     ILDTPIMIGAMSFGALSKETKIALAMGATLAGTAANTGEGGMLPEERKYASKLIAQYA
                     SGRFGVSAQYLNNCEAIEIKIGQGAKSGMGGHLLGEKVTAEVSKIRMIPPGTDALSPA
                     RHMDIVGPEDLNMKISQLREISDWKVPIMVKFGSGRVSDDVKIAAKAGADIVVVDGMQ
                     GGTGASPEVATEHSGVPTIAAIVQADEALKHVNLRDQVSLVAAGGIRNGADVAKAIAL
                     GADACYIATSALVSIGCRVCQMCFAGTCRKGIATQNPNLRRRLDYIEGGKRVARYIDV
                     MTEEAVMLTQQAGNTDLLKLEKDDLRALTVESSIMTGVKMTGVEAPIRG"
     gene            complement(3637..4734)
                     /locus_tag="PKK85_00245"
     CDS             complement(3637..4734)
                     /locus_tag="PKK85_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNS24486.1"
                     /translation="MSNQENVVAMVGTPCQIIAAENMSYFQDYLGESPVDIKVGLFCM
                     ENFSYHYLNQLLEERNIQLKDVSECRVEKGHMWFYLTDGQIQKIPLKEVKTCMRKNCE
                     VCMDYTSELSDLSVGSVGSPDGWSTVIARTVKGLELLKKAEEDNYIETKPMTDSGIAL
                     IKKLACQKKEENKKKIKKRESVARPVIYRRFITDKQFREEVADCQFEDLKADVIDVGT
                     CVLCGSCEYICPDDIIKIEDRKPRIKGQCPPDCNLCYVACPRTYLPVEVASPDLDQKP
                     LGDYMKIVSAKASLVKGQDGGVVTALLSYALSDGIVDEALVVDKSSAEPWRPKVKLTS
                     NIDDVLRASGTKYAACPVFKGLKSKNIKEEL"
     gene            complement(4739..>5386)
                     /locus_tag="PKK85_00250"
     CDS             complement(4739..>5386)
                     /locus_tag="PKK85_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GXGXG domain-containing protein"
                     /protein_id="HNS24487.1"
                     /translation="EVNRTLKEKASKYKKIVIENPNAAHYLVAGLTEPIDVEIAGSAG
                     YFAATMIHGARVHIQGNAGWFPADNMTEGEVIIEGSAGDGVGQGIYDGTVVVKKNVGS
                     RTGMIMKNGTIIVGGNSGFMNGMFMMGGRIIVLGDIAKDAGESMMGGTIYVGGEIESL
                     GKNAKVKELEAQELDELRELLTSYGFQLEEAQYQKFRKIVPWSKEPFYGKETEEG"
     assembly_gap    5387..5396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5440..6357)
                     /locus_tag="PKK85_00255"
     CDS             complement(5440..6357)
                     /locus_tag="PKK85_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HNS24488.1"
                     /translation="MCGIAGVVYKDKKLHPVGDALTKMLDALQHRGSDSAGFALYGGL
                     GLDENEYLLNIVVKEKPGLLEKVKETVNQASPIKTEEIIPSVENYKIFRCRVTLNSFS
                     ELKTMITAIDAVEDVVVLSGAHSFEMIKDVGLVKDIAQRYDTYSKMGTHGIGHIRFST
                     ESIVDRYHAHPFQSYIIPDITVVHNGQITNYWKIRDPLERKGHVFETTNDTECIVHYI
                     ADKLSQDYPLEEALEQSVKDMDGPFSYIVGTPHGVGIAKDQLGLRPGVMAENDEVFAI
                     ASEEVSLREVVDTREIDQISPGEVRAYTI"
     gene            complement(6480..6638)
                     /locus_tag="PKK85_00260"
     CDS             complement(6480..6638)
                     /locus_tag="PKK85_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24489.1"
                     /translation="MVNKEILYLQGDVTKDWGLVARLWKIRTEYRGGTGKSCSEVAHC
                     QGICHLPL"
     gene            6902..7156
                     /locus_tag="PKK85_00265"
     CDS             6902..7156
                     /locus_tag="PKK85_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24490.1"
                     /translation="MEKLTMGLIVMIVLLGGFLSYLGITMDTSDFSLNLTNNTTDYPT
                     VTKINNTRTITSPNQTTNRTRPGNNTRPGNDTKPGNDTDL"
     gene            7175..7438
                     /locus_tag="PKK85_00270"
     CDS             7175..7438
                     /locus_tag="PKK85_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24491.1"
                     /translation="MEKLTIGLFFMMLLMVGFNSYLVATMDSSDITMNSTNLSTSYFS
                     SNHSINHTTRVNNTRIVTKPSTNESNATDRARVNYYKKSLGIT"
     gene            complement(7733..7894)
                     /locus_tag="PKK85_00275"
     CDS             complement(7733..7894)
                     /locus_tag="PKK85_00275"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24492.1"
                     /translation="MKTMNDDSGFVRGGAAFAIGQIGGEEAEEALEAALNDKSSYVRK
                     VAQNYLSRG"
BASE COUNT         1893 a         2064 c         1823 g         2117 t
ORIGIN      
        1 ccggaacctg ggagaagcct tgcggaggat tgatgaggga gctgccatga tccggaccaa
       61 gggtgaagct ggtaccggta acgtggtgga agcagtacgg cacatgcgca tgatccaggg
      121 taccatccgg gacctgaagg acaagaccga ggaggaactg tggggagtag cccgggaaat
      181 tgaggctcca ctggaactgg ttaaagaaac ccagaaacag ggccgactac ccgtggttaa
      241 cttcgctgct ggtggagtgg ccactccagc tgacgccgcc ctgatgatgc agctaggagc
      301 tgacggagtt ttcgtgggaa gtggtatctt caaatccgaa aagccggagt tagtggccca
      361 ggccattgta gaggccacca cccactacaa caacgccgag gtcctggcca acgtctcccg
      421 gggattgggc aaggccatgc ccggcttgga gatgagcagc atccctgccg aagaaagatt
      481 acaggaccgt ggatggtaga tccacccaca tttttctcat ttttctaatc tagaatcaaa
      541 atagtaatta aaccaactaa ccatggagtg ttggttctga agttatgtct ttttcccata
      601 ttccatgatc attgaattga ggaaaatatt caggagatgg aaaagaagca cctacaacga
      661 tcaccagggt tattctggtt aaaataaatc tcacacacct ttttttagga aacataccaa
      721 caccatcacc cggagggaca tggatctagt aagtccctta gaaaaatgaa taaataggca
      781 cccctagggt gccccgcctg ctccacctat attctggcca gtggtggcat cgatctctat
      841 ctggcctact gcctttccct tgtaaatgat ggggacgatg tagaccggtt taccatttat
      901 ggaggtgagt tggggtgttc cagcactggc cccttcctgt tcaatgtact tcttggccag
      961 ttcctgggcc tgggtggcgg tgatgttggt gtggctggtg gtgttttgat tcaggttgga
     1021 gttgttgttt gtggtgttgt tcttattgcc ggtgtccagg cacccggaaa ctgcaaccac
     1081 taggatcgtt aaaataatta aaccaatcat ggcttttttt atcaatttat cacctacggt
     1141 gattcatttc ttagtacatt ttttatcact attactatta attattttct aaaattccgc
     1201 attctaaatc cagttataat aaaacctccc taatcttcaa aatactactt aagaggctgt
     1261 ttctcgggac aatagtaggt ggtgcgccct ccaactttga tcacctccaa tgggtctcct
     1321 tccggacatc ttcctcccgg gtaacggtgg ggtaaaaggt aggaatcagg gaagtcccgg
     1381 ggactgtcct ggcactctat ggccttctcc agcaccctcc tcatctggta gtagaggtca
     1441 cccacatcgc ttttactgag ctgatgggca ggagtttcag ggtgcaagcc acattggtac
     1501 aggatctcat cagagtagag gttaccaatc ccggcgacaa agttctgatt cagtagtaag
     1561 ggtttgatca aacccctccg atccttaatg gtcttgtaaa aggctttgaa tgagatgtca
     1621 agggcatcct cacccaaacc cttcccagcg atgaaatcat caggatcagg ggtcaatccc
     1681 agcttcccaa acttccgggc atcatcaaat gccaagtgga agccgttatg gaaactgatg
     1741 agcatcctga catacttgga gcggttatgg tggaagtaat ggaggtagcc ggtcatccca
     1801 aagtgcatga caaggtactg gttctcatcc aactgggcaa agaggtattt accatgcctg
     1861 cggctggaga tgaattcatg gccttccagg gacccttcaa atctccgggg atctgcgtac
     1921 acaaccagtt ctggactgac cacctccacc ttctttatca tctggttgag ggcgtgctga
     1981 tctaagtagc gcttgaaaat ctccacactg ggtagttctg gcatgctgga tactccgagt
     2041 aggcttaatt actactggat aggagcagca taaaataaat tcttctatct taaaaaaaat
     2101 ctactcaaaa aaaaggtaag ggttttcagg ggatcattat cccctgatgg gtgcttccac
     2161 tcccgtcatc ttaaccccgg tcatgatgga tgattccaca gtaagggccc ggaggtcatc
     2221 cttttccagt ttcaaaagat cggtgttacc agcctgctgg gttagcatca cagcctcctc
     2281 ggtcatgaca tctatgtaac gggctacccg tttaccaccc tcaatgtaat ccagcctgcg
     2341 acgcaggttg gggttctggg tggcgatacc cttacggcag gtaccggcga agcacatctg
     2401 acacacccgg cagccaatac ttaccagggc actggtggca atgtaacagg catcggcacc
     2461 cagggcaatg gccttggcca cgtcagcacc attccggatt ccgccggctg ccactaggct
     2521 gacctggtcc cggaggttga catgtttcag ggcctcgtca gcctgcacaa tggcggctat
     2581 ggtgggtacc ccggagtgct cggtggccac ctcgggtgag gccccggttc ctccctgcat
     2641 accatccacc actacgatat ctgctccggc cttggccgcg attttaacat cgtcactgac
     2701 cctgccactg ccgaatttaa ccatgatagg cactttccag tcagatatct cccttaactg
     2761 ggagatcttc atgttcaggt cctcggggcc cacgatgtcc atgtgccggg ctgggctcaa
     2821 ggcatcggta cctggaggga tcatgcgtat cttggaaacc tcggcggtga ccttctctcc
     2881 taaaaggtga cctcccatcc ctgacttggc tccctggccg atcttgatct cgatggcctc
     2941 acagttgttg aggtactggg ctgaaacccc gaatcggcct gatgcatatt gggcgatgag
     3001 tttagaagca tatttacgct cttcaggcaa catacctccc tccccggtgt tggcagctgt
     3061 gcctgccagg gtggcaccca tggccagggc gatcttggtc tccttggaaa gagcaccgaa
     3121 tgacatggcc ccaatcatga tgggtgtatc cagtataagg gggttttcag cgtaacggtc
     3181 acctagaact accctggtgt tgcagggctc ccggtacttg tcgatgggtg gccgggatgc
     3241 ctgggctggt acaatgacca agtcatcgaa ggtgggtatc acccgagtag caccgcagcc
     3301 ccggacttta taggagccct cagtggcctt tctgtttatc tcggcgagat ccgtgaggga
     3361 ccacacattt ctacggtcct ctggcacaga gttaacttcg atagcattgt taggacacat
     3421 ttcctcgcag atacggcacc ccacgcactg ttcatggtgt atggggtagg gttcatcatc
     3481 gattatctcg taaacttcgt ggggacagtt attgtaacag gagtaacagt tacgacacag
     3541 gttctcatcc cgatcatcgc acatgtacca gcaacaaccc ggccggtcga agtttctccg
     3601 gcaaagttcg tggtttcttt caactttaaa tggcaactat aactcctcct ttatgttttt
     3661 actcttaagc cccttgaaca ctgggcatgc agcgtattta gtaccggagg ccctgagtac
     3721 atcgtcaatg ttactggtga gtttaacttt aggcctccag ggttcggcgc tacttttatc
     3781 caccaccagg gcctcgtcca caattccatc cgaaagtgcg tagcttaaaa gagcggtaac
     3841 cactccacca tcctggccct taactagtga ggccttagct gacacgatct tcatgtaatc
     3901 ccccagtggc ttctgatcca ggtctggact ggccacttcc accggcagat aggtccgggg
     3961 acaggccacg tagcacaagt tacaatccgg ggggcactgg ccctttatcc ggggtttacg
     4021 gtcctcaatc tttattatat catcggggca gatgtattca caacttccgc agaggacaca
     4081 ggtacccacg tcgatgacat cggccttgag gtcttcgaac tggcagtcag ccacttcctc
     4141 ccggaactgt ttatcagtta tgaatcggcg gtaaataact ggccgggcca cactttcccg
     4201 ttttttaatc tttttcttgt tctcttcctt tttctgacag gctaacttct tgataagtgc
     4261 tattccagag tcagtcatgg gcttcgtttc gatgtagtta tcttcctcag cttttttgag
     4321 taactccagc cccttaacag tccgggcgat tacggtggac catccatccg gggatcccac
     4381 cgaacccact gacaagtccg ataactcgga ggtgtaatcc atgcacacct cacagttttt
     4441 gcgcatgcag gtcttaactt ccttcagagg gatcttctgg atctggccgt ctgtgaggta
     4501 gaaccacatg tgtccctttt ccacccggca ttcggaaaca tcctttaatt gtatattgcg
     4561 ctcctctaag agctggttca agtagtggta ggagaagttc tccatacaga agagtcctac
     4621 cttgatgtcc actggggact ctcccaagta gtcctggaag taactcatat tctcggcggc
     4681 tatgatctgg cagggcgtgc ctaccatggc cactacattt tcctgattac tcataaactc
     4741 atccctcttc tgtttctttt ccgtagaagg gttctttact ccagggtact atcttccgga
     4801 atttctggta ctgggcctct tccagttgga atccataact ggttagaagt tctctaagct
     4861 catccagctc ctgagcttcc agttccttta ccttggcgtt tttacccagg ctttctattt
     4921 ctccacccac gtagatggtc cctcccatca tggattcacc ggcgtccttg gctatatctc
     4981 ccaggacgat gatgcgtcca cccatcatga acattccatt catgaacccg gagttaccac
     5041 ctacaatgat ggtcccgttt ttcattatca ttccggtacg ggatcccaca ttctttttca
     5101 ccaccactgt accatcgtat attccctggc ccacgccgtc accagcggat ccttcaatta
     5161 taacttctcc ttcggtcatg ttatcggcgg ggaaccagcc agcattgccc tggatgtgga
     5221 cccgcgcccc atggatcatg gtggctgcga aataaccagc cgaaccagct atctccacgt
     5281 ctataggttc agtgagacct gccaccaggt aatgggcggc gttggggttt tcaatgacga
     5341 tctttttata tttgctagcc ttctctttaa gggtacggtt aacttcnnnn nnnnnngtcc
     5401 cccggcatca attgtgaatt cctgcatgat gatcaccttt tatatggtgt aggccctcac
     5461 ttcccctggt gatatctggt ctatttcccg ggtgtccacc acttccctca aggacacctc
     5521 ttccgaggct atggcgaata cttcatcgtt ttcagccatt accccgggcc tcagtcccaa
     5581 ctggtccttg gcgattccca caccatgggg ggtgcccacg atgtaggaga agggtccgtc
     5641 catgtccttc accgactgct ccagagcctc ctctaagggg tagtcctggg ataacttgtc
     5701 ggcgatgtag tgcactatgc actcagtgtc gttggtggtt tcgaagacat gtcctttacg
     5761 ctccaggggg tctctgatct tccagtagtt tgttatttgg ccgttgtgta ctacggtgat
     5821 gtcggggatg atgtaactct ggaaggggtg ggcgtggtag cggtccacaa tactttcggt
     5881 ggagaatcgg atgtgtccga tgccatgtgt ccccattttg gagtaggtgt cataacgctg
     5941 ggctatgtcc ttaaccagac ccacatcctt gatcatctcg aaggaatgag ccccactgag
     6001 gacaaccaca tcctcaacag catcaatagc cgtgatcatg gtttttaact cggaaaaaga
     6061 attcaaagtg accctgcacc ggaaaatctt gtagttttcc actgagggaa tgatctcctc
     6121 ggtttttatg gggctggcct ggtttactgt ttctttcacc ttttccaaca gtccaggttt
     6181 ctctttaacc actatattca agaggtactc gttttcatcc aatcccaaac ctccatatag
     6241 ggcgaaccct gctgagtcag acccccggtg ctgcagagca tcaagcatct tggtcagtgc
     6301 atcgcctaca gggtgaagtt ttttatcctt ataaaccact cccgctattc cacacaattt
     6361 gctacctcct taggtaaata ttcataaatt tctgtatttt cgtatatatt tagggtaatc
     6421 cataggttcc tcggccctca ctttttccct ggaagaggtc ttctggcttt gggtaaactt
     6481 cacaatggga ggtgacagat tccctgacag tgagccactt cagagcagga ttttcctgta
     6541 ccacctcggt actcagttcg tatcttccag agcctggcta ccaatcccca atcttttgta
     6601 acatcgccct gcaagtatag aatctcttta ttaaccattc aattccttcc taggggggtg
     6661 ttggctggat atacttggaa atgtgtccct tatatactta ccggaaaata atttccgacc
     6721 agggcgtacc ttgaaatata ataccttata atgaaaattt ttgtacgaat gtttaattga
     6781 aatagattgt tgataatata tttgaacatt tatataaatc tttccttggg gtggtgggca
     6841 caatctttat ttagttaatg ggtacaaccc ataaagtaag atagatatag gagattaact
     6901 aatggaaaag cttaccatgg gtctcatcgt tatgatcgta ctgttgggtg ggttcctgag
     6961 ctatctgggc attaccatgg acaccagtga cttcagtctt aacttgacca acaacaccac
     7021 tgactaccct accgttacta agataaacaa cacccgcacc atcaccagcc ccaaccagac
     7081 caccaacaga accagaccag ggaataacac ccggccagga aatgatacta aacctggaaa
     7141 cgatactgat ctgtaaatct gtgagtgatt agatatggaa aagctgacca tcggactatt
     7201 ttttatgatg ttactcatgg tgggattcaa cagctacctg gtggccacca tggattccag
     7261 tgatatcacc atgaacagca ccaacctcag taccagctac ttcagcagca atcactccat
     7321 caatcacacc acccgggtga acaacactcg catcgtgacc aaaccaagca ctaacgaaag
     7381 caacgccacg gaccgtgccc gggtgaacta ctacaaaaaa agtctgggca taacctaaaa
     7441 aaaagattta aaaagtcatc ttttattcta gaaaaagcat ccaatctccc ttaagagaat
     7501 tggcaaaaaa aatccagggg aggagaagtt tacaacttca gccgcctctt aaatggctta
     7561 ttccatgtat gaacacagga actatctttt cttcaccatc ccatccttgc caacccacga
     7621 cacacacctc ccagttatgg tagggaatga cggttatgac atctcccata gctcaagatg
     7681 ggggatattg aaaatagatg gaatcaagcc cccacaacgg ggtaatcagc catcatcccc
     7741 ggcttaagta gttctgggct acttttcgta cgtaactgct tttatcattg agagctgctt
     7801 ccaaggcttc ttcagcctct tccccaccta tctgtcctat ggcaaaggca gctcctcccc
     7861 ttacaaagcc actatcatca ttcatagtct tgataagggg tttcagg
//
LOCUS       DAOQVW010000006         7427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_10985_length_7427_cov_2.895279, whole genome shotgun sequence.
ACCESSION   DAOQVW010000006 DAOQVW010000000
VERSION     DAOQVW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10985_length_7427_cov_2.895279"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKK85_00280"
     CDS             complement(<1..418)
                     /locus_tag="PKK85_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012956429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HNS24493.1"
                     /translation="MKVWIDIVNAPHVRFFKDIIEYLDNEGEEVFVTARKFGDVHRLL
                     DLFGIEYTLVGKHGATLEDKLLRSTSRVFELSKLIAQEKPDVAVSKHSIELPRISFGL
                     GIPSVYVLDNEHALAANKLTLPLCDQIILPRTIDVWR"
     gene            complement(469..1119)
                     /gene="hypB"
                     /locus_tag="PKK85_00285"
     CDS             complement(469..1119)
                     /gene="hypB"
                     /locus_tag="PKK85_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNS24494.1"
                     /translation="MHKIADVEIQHDIMVANRKLARNNQRLLDQNGIFAVDVLGAIGS
                     GKTSLIETLIDHLDHKVGVIAGDVISKYDAGRFESHNVPVVGLNTGKECHLDAHLVKH
                     ALEDMPLEDIDLLFIENVGNLICPVDFELGSHLRMVVISVSEGDDTVEKHPLIFQDAD
                     LVVINKVDLAEAVGASVEKMVNDVKELNPGVKVITSSIKMGKGLDEIISTIESYVL"
     gene            complement(1121..1492)
                     /gene="hypA"
                     /locus_tag="PKK85_00290"
     CDS             complement(1121..1492)
                     /gene="hypA"
                     /locus_tag="PKK85_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNS24495.1"
                     /translation="MHELSMAQAIVDTVLDAAQKNEAQDVLEVTIEIGQITMLNPEQL
                     KFLLNVLVEGTLMEGAQIIIEEIPVEIECQLCEFKGEANTDGSDHYLTIVLCPECGAR
                     DVEVTKGRECNVKNIRIEKVD"
     gene            1701..2564
                     /locus_tag="PKK85_00295"
     CDS             1701..2564
                     /locus_tag="PKK85_00295"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNS24496.1"
                     /translation="MIIGGSASQKLAAQIAKKMGEPLCPLETRKFPDGERYIRINGKL
                     EDNVTVVQSTGYPQDENLIELFLILKTLQNMDVTNIRAVIPYFGYGRQERSFNPGEAI
                     SASVIADLIEFSGVSEVFCINLHETSICNLFNVPTHNLSAMPVIAEYLDNCRDMPLLL
                     APDHGAINFAMDIANILGSEYDHLEKKRISPDLVETKTKNLDVEGREVVIIDDIISTG
                     GTIVNAIKMLKENGASNITVSCVHPVLVDDALLKIFAAGADEVIGTDTLKSEISVVSV
                     AELVAEALKSK"
     gene            complement(2614..4197)
                     /locus_tag="PKK85_00300"
     CDS             complement(2614..4197)
                     /locus_tag="PKK85_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS24497.1"
                     /translation="MDSLLFEEMDLSLKIKRAINDLGFEEATPIQSLVIPPILEGKDV
                     IGQAQTGTGKTASFGIPVLEMVDPNDDSLQAVILCPTRELAIQVAEELKKLSKYEKTK
                     VIPIYGGQPIERQIKALKKGVQIIIGTPGRVMDHMYRRTLSLGKVKIIILDEADEMLD
                     MGFREDIEFVLGKMPSERQMLLFSATMSKAILKLTKKYQKDPTFLKVAHQELTVPQIE
                     QVYFEVREKNKLDLLSRLIDLHELQLSLVFCNTKRKVDKLVNHLQTRGYMAGGLHGDM
                     SQNQRDRVMAKFRKGQIEVLVATDVAARGIDVDDVEAVFNYDVPNDDEYYVHRIGRTG
                     RAGKTGQAFTFVAGKEIYKLRDIQRYTKVRIKQLQIPSLQEVEKLKNHRFLENLKHMI
                     NNEYLDSEIKMVEKLMEEDYSSLEVAAALMKMSLDKKNSYESKSSSVFGDTGAQPGKV
                     RFFVNLGRKDNVKAKDIVKVIHEVSGLSSTSIGRIDVLDKFSFLELPLEYAWEISEAL
                     EGTRIKGKRVNLEPANKKN"
     gene            complement(4405..6312)
                     /gene="lonB"
                     /locus_tag="PKK85_00305"
     CDS             complement(4405..6312)
                     /gene="lonB"
                     /locus_tag="PKK85_00305"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HNS24498.1"
                     /translation="MEISNPEKSSETDETLEFSGYKTSEDIDVPERIIDQIIGQEEAV
                     ETVKKAAKQRRNVLLIGEPGVGKSMLAKGMAELLPPEELQDILVYPNIDDNHNPLIGV
                     MPAGEGKTVVTNYKLKAKSQDERKNMFMIAIISLIMVIGFVLQQFLAAIIAAGIVFLA
                     MQQMKPRSTLMVPKLLVNNEKNKMAPFIDATGAHAGALLGDVRHDPYQSGGLGTPAHE
                     RVEAGMIHKASKGVLYVDEIGGMKMKTQQELLTAMQEKKYSITGQSETSSGAMVRSQA
                     VPCDFVLVASGNLHVLEGMHPALRSRIRGYGYEVYMKDTMPDNQENRDKLVQFVAQEV
                     KKDGRIPHFGREAVEEIIREAQRRAGKKESLTLKLRDLGGLVRAAGDIAKGEHKDEVA
                     LEHVISAKKLARTLEQQIADRYILQKKRYRVFRSEGGEVGRVNGLAIIGDRSGIIMPI
                     AAEAAPAQSKEEGKIIATGKLGEIAREAVQNVSALIKKHTGTDIASYDIHIQFLQSYE
                     GVEGDSASVSVATAVVSALENIPVDQAVALTGSLSVRGDVLPVGGVTGKIEAAAEAGI
                     KKVLIPQSNMEDVFIEERYQKEIEIIPVETLSDVLEHTLVGKGKKSLMEKMKKIADIV
                     PQGILKQPATH"
     gene            6580..>7427
                     /gene="cobQ"
                     /locus_tag="PKK85_00310"
     CDS             6580..>7427
                     /gene="cobQ"
                     /locus_tag="PKK85_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051921 - adenosylcobyric acid synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HNS24499.1"
                     /translation="MKCLMVQGTSSNAGKSVVVAALCRILSKRGFRVVPFKSQNMSLN
                     SFTTQENREIAMAQVFQAEAAGLEPHHHMNPILLKPKEDFTSQVIVHGLPAGDMNFYH
                     YQNNFRDEALKAIKKSLYELEKEYDILIMEGAGSPAEINMLDRDMANMPIARLADADV
                     ILVADIDRGGVFASIAGTFQLLPPEDRARIRGIIINKFRGNLDILVPGIRQIEKIVGV
                     PVLGVLPYDKQLKLPEEDSASLSEHKYPNHGRIKVGIMRLPRISNFTDLDPLEYEPDI
                     GIRLVE"
BASE COUNT         2047 a         1691 c         1422 g         2267 t
ORIGIN      
        1 cacgccacac gtcaatggtc cggggtagta tgatctgatc acaaagtgga agggtgagtt
       61 tattagcagc cagtgcatgt tcattgtcca acacatacac actgggaatt cccaatccaa
      121 atgaaatcct aggaagttca atggaatgtt tagaaacagc aacatccggt ttttcttggg
      181 ctatgagttt ggaaagttca aaaactcgac tggtactgcg aagcagctta tcctccaatg
      241 tggcgccatg tttcccgact aaagtatatt caataccaaa aaggtccaga aggcgatgca
      301 catccccaaa cttacgggca gtgacgaaaa cctcttcacc ttcattgtca aggtactcta
      361 taatgtcttt gaagaaccta acgtgggggg cgttgacaat atcaatccag accttcaatt
      421 catcaccaaa tcgcgttgag tgccaaataa attctgttgt agaagaatct acagcacata
      481 ggactctata gtcgatatga tctcatccaa gcctttaccc atttttatgc tgctggttat
      541 aactttcaca ccaggattga gttccttcac atcattaacc atcttttcca cactggcccc
      601 cacagcttct gcaagatcca ccttatttat taccaccaga tcggcatcct ggaatataag
      661 aggatgtttt tccactgtgt catctccctc actgacactg ataaccacca tacgtaagtg
      721 ggaacccaat tcgaaatcta ctgggcagat caagttcccc acattctcaa taaacaacaa
      781 atcgatgtct tccagaggca tatcttccag ggcatgttta accagatgtg catctagatg
      841 acattctttt ccagtgttta atcctacaac cggcacatta tgggattcaa aacgccctgc
      901 atcgtacttg ctgatgacat caccggcaat tacccctact ttatggtcca gatgatcaat
      961 taacgtttca atcagggatg tttttccgga gcctatggca cccaggacat ccaccgcaaa
     1021 aattccattt tgatccaata atctctgatt attacgggcc aattttctgt tggcaaccat
     1081 tatatcatgt tgaatctcca catcagcgat tttatgcatc ctaatcaacc ttctctatcc
     1141 taatattttt aacattacat tctcgtccct tggtaacttc cacatctcta gctccacatt
     1201 ctgggcatag gactatggtt aaatagtgat cagaaccatc agtatttgct tctcccttaa
     1261 attcacataa ttggcactct atctccactg gaatttcttc gataattatc tgagctcctt
     1321 ccatcaaggt accttccact agtacgttca acaaaaattt cagttgttcc ggattgagca
     1381 tggtgatttg tccaatttca atggtaactt ccagaacatc ctgagcttca ttcttctggg
     1441 cagcatccag gacggtgtcc acgatggctt gtgccatgga aagttcgtgc ataactttac
     1501 ccctatttag tactaactta tcttactatg gttaaagact tactatggtt taaagagagc
     1561 ttattaataa agttaacaaa tagttgaaat ataatgtttt attgtctcat aatgagcttt
     1621 gtggttttaa tagaaacccc gaaataacgt taagacatta aactcgcata atgattagtc
     1681 atggagagta ggtggtttta ttgatcatag gcggatctgc atcacagaaa ctggcagccc
     1741 agattgcaaa aaagatggga gaacccctct gcccattgga aacacgtaaa ttccctgatg
     1801 gtgaacgcta catacgtatt aacggaaagt tagaagataa cgttactgta gtgcagtcta
     1861 ccggttaccc gcaggatgaa aatctcatag aactcttttt aattctaaaa acacttcaga
     1921 acatggatgt tacaaatatc agggcggtta ttccctattt tggttacggc agacaggaaa
     1981 gaagtttcaa cccgggtgaa gccatttctg cctcggtgat tgcagatcta atagaatttt
     2041 ccggggtgtc cgaggtattc tgtattaatc tccatgaaac gagcatatgt aacctgttca
     2101 atgtacccac ccataacttg tcagccatgc ctgttatcgc ggaatattta gataactgca
     2161 gggatatgcc actactttta gccccagacc atggggccat caattttgcc atggacattg
     2221 ccaatatatt gggcagtgaa tatgaccact tggaaaaaaa acgtatttca ccagacctag
     2281 ttgaaaccaa aactaaaaac ttggatgtcg agggaagaga agtggttatc attgacgaca
     2341 tcatcagtac cggtggaacc atagtcaacg ccataaaaat gttaaaagaa aatggggctt
     2401 ccaacataac ggttagctgt gtacacccag tcctggtgga cgatgccctt ttaaaaatat
     2461 tcgctgctgg tgcagacgaa gtgatcggta ctgatacctt aaaatcagag attagcgtag
     2521 tttcagtggc tgagttggtg gcagaagccc tgaagagtaa atagatgctt ctattttcta
     2581 caaaacattt tattcattag tatttggctt ttattagttc ttcttgttgg ctggctccag
     2641 attaaccctt ttgcccttta ttctggttcc ttccagagcc tcagatattt cccaggcgta
     2701 ttccagtggc agttctaaga atgaaaactt gtccaacaca tcaatacgac ctatactagt
     2761 ggatgaaagt cctgaaactt catgaatgac tttaacaata tctttagctt tcacattatc
     2821 cttgcggccc aagttaacga aaaatctgac cttacctggt tgagctcctg tatcaccaaa
     2881 aactgaagaa gattttgatt cataactgtt ctttttgtcc aacgacattt tcataagagc
     2941 tgccgccact tctagagaac tataatcctc ttccatcaac ttttcaacca ttttaatctc
     3001 tgaatccaga tattcattgt taatcatatg ttttaaattc tccaagaaac ggtgattctt
     3061 aagtttctcc acttcttgaa gtgatggaat ctgcaattgc ttaatacgaa ccttagtgta
     3121 acgttggata tctcttaact tgtaaatttc ctttccagct acgaatgtga aggcttgacc
     3181 ggtttttcca gctctccccg ttctaccaat acggtggacg tagtactcgt catcattggg
     3241 aacatcgtag ttgaaaacag cttccacatc atccacgtca attccccggg ctgctacatc
     3301 cgtagccacc agaacttcta tttgtccttt tctgaatttt gccattacac gatctctttg
     3361 gttctgactc atgtccccat gcaaacctcc agccatatac cctctggtct gtaaatggtt
     3421 caccagttta tcaacttttc ttttagtgtt acaaaatact agggacaatt gaagttcgtg
     3481 caaatctatt agtcgggata gtaggtccag tttattcttc tcccgcacct caaagtaaac
     3541 ttgttctatt tggggtacgg tgagttcctg atgggcaact ttcaaaaatg taggatcttt
     3601 ctggtacttc ttagttaact ttaatatggc cttggacatt gtagcagaaa agaggagcat
     3661 ctgtctttct gaaggcattt tgcccaggac gaattcaata tcctcccgga accccatatc
     3721 gagcatttca tctgcttcat ccaatattat tatcttaacc tttcctaggg acagggtgcg
     3781 acggtacata tgatccatca cccggccagg agtcccaatg ataatctgaa cccctttttt
     3841 aagggcttta atctgtcttt ctatgggttg tcctccataa attggtataa cttttgtttt
     3901 ttcgtatttt gaaagttttt ttagttcttc tgcaacttga atagccagtt ccctcgtggg
     3961 acagagtatt acagcttgta aactatcatc attagggtca accatttcta ataccggtat
     4021 cccaaatgat gcagtttttc cggttccagt ttgggcctga cctatcacat cttttccttc
     4081 cagaatagga ggaataacta aagactgaat tggagttgct tcttcaaatc caaggtcatt
     4141 aatagctctt ttaattttaa gagataaatc catttcttca aataataaac tatccataat
     4201 taatcttcct tatacaaatt aatccatgta tcagactatt aattactcaa actcaaaatc
     4261 agcttaaaat aatcaaaaat ctattcttat tagcgtggtg aactacttat aatgatccct
     4321 aagttaaaaa atttagttaa aatttggttt aaaaaaaaag ttgtggcctc tactaaaaag
     4381 tagaggtttg atgggggtta ggttttagtg ggttgctggt tgtttcagta tcccctgtgg
     4441 aacaatgtct gcgatcttct tcattttctc catgagactt tttttacctt taccaaccag
     4501 agtgtgctcc aggacatcgc tgagagtttc taccggtatg atctcaattt ctttctggta
     4561 gcgttcctca ataaacacgt cttccatgtt ggattggggt atgagaactt ttttaatacc
     4621 agcttctgct gcagcttcaa tcttgccggt gacaccccct actgggagca catccccccg
     4681 tacacttaaa gatccagtta aagccaccgc ctgatcaacg ggaatgtttt ccagggcgga
     4741 taccacggca gtggccactg aaacactggc actgtcacct tccactccct cataggactg
     4801 gaggaactgg atatgaatat cgtaactggc tatgtctgtc ccggtgtgtt ttttaataag
     4861 tgcactaacg ttttggactg cttctcgagc tatttcccct agttttccag tggctatgat
     4921 tttaccctct tctttacttt gagcaggggc agcttctgct gctatgggca taattatccc
     4981 actacgatct ccaattatgg ccaaaccatt cacgcggccc acttcaccgc cttcagatct
     5041 gaaaacccga taacgttttt tctggagaat gtaacggtcg gcgatctgtt gttccaaggt
     5101 tcttgccagc tttttggcac tgataacgtg ttccagagct acctcatctt tatgttctcc
     5161 tttggctatg tcaccggctg ctcgaaccag ccctcccaga tcgcgcagct ttaaggttaa
     5221 tgactctttc ttgccagcac gacgttgagc ttcacgtatt atctcttcca cagcttctcg
     5281 tccaaaatga ggtattctgc catctttttt aacttcctgg gccacgaact gaaccagttt
     5341 atcacggttt tcctggttat ccggcatggt gtccttcata tacacttcat aaccgtaacc
     5401 ccttattctg gaccttaaag ccgggtgcat accctccagt acgtggaggt taccactggc
     5461 aaccaggaca aaatcacaag gaacagcctg agaacggacc attgctccgc tactagtctc
     5521 actttgacct gtgattgaat atttcttctc ttgcatggca gttagaagtt cctgctgggt
     5581 tttcatcttc atgccaccga tttcatccac gtagagtaca cccttactgg ccttgtggat
     5641 catgcctgcc tccacccgtt catgggcagg ggtacccaat cccccagatt ggtagggatc
     5701 gtgcctcaca tctccaagaa gagccccggc gtgtgcaccg gtagcatcaa tgaatggggc
     5761 catcttattc ttttcattat ttaccaacag tttaggcacc attaaggtgc tccggggttt
     5821 catctgctgc atagccagga aaacaattcc cgcagcaatg atggctgcca agaactgttg
     5881 gagcacgaat cctataacca ttatcaaact gataatagct atcatgaaca tgttttttcg
     5941 ctcatcctgt gatttggctt tcaatttata atttgtaacc actgttttac cctcaccagc
     6001 cggcataaca ccaatgaggg ggttgtgatt atcatctatg ttgggatata ccagtatatc
     6061 ttgtagttcc tcgggaggta agagttccgc catcccctta gctagcatgg actttcccac
     6121 accgggttct ccaattaaca gaacatttcg gcgctgttta gctgcctttt tgactgtctc
     6181 cacggcctct tcctggccga ttatctgatc gatgatcctt tccgggacat caatatcctc
     6241 ggaagttttg taaccactaa attctaaggt ttcatctgtc tcagaacttt tttctgggtt
     6301 agaaatttcc atgggctttt taaacctcca taaatttgga ttaagtaaaa attaaaactt
     6361 ttttttagtt tgatatacag taactggtat atgatatctt atataagaac atttgcctaa
     6421 gaacattttt cttataatat tctcaagctt cttataagta ggatgtggat aaaatctaat
     6481 aaaaagtata ggtagagtag taaagagtac tacttcagtg ctgcaattga ttttattaga
     6541 tgaacacaat ttgtgttaat attacaagtt agtgataaaa tgaagtgtct catggttcag
     6601 ggaacatcat ccaatgcagg aaaaagcgtg gttgtagccg ccctatgtag aatactgtct
     6661 aaaagaggat tcagggtggt tccttttaaa tcacaaaaca tgtccctgaa ttcattcacc
     6721 acccaagaaa accgggaaat agccatggca caagttttcc aggcggaagc cgctggcttg
     6781 gaaccccatc atcatatgaa ccccatcctc ttgaagccca aggaagactt cacatctcaa
     6841 gtcattgtcc atggactacc agccggtgac atgaattttt atcactacca gaataacttt
     6901 agggacgaag ctttaaaagc cattaaaaaa tctctgtacg aattagaaaa agagtatgac
     6961 atacttatta tggaaggagc tggttcaccg gctgagatca acatgttgga tcgagatatg
     7021 gccaacatgc ccattgcccg tctggctgat gcagatgtga tcctggtggc agacatagat
     7081 cgcggaggag tttttgcatc catagctggt acttttcagc ttttaccccc tgaagatcgg
     7141 gcccggatca ggggaattat aatcaacaag tttcgaggta acttggacat tttagtaccg
     7201 ggaatccgcc agatcgagaa aattgtggga gtgcctgttc tgggcgtatt gccatatgat
     7261 aaacaactga aactgccaga agaggattcg gcatccctat cagaacataa ataccctaac
     7321 catggccgga ttaaggtggg aataatgagg ctgccacgca tatccaactt cacggatctg
     7381 gatcccctgg agtatgaacc agatattgga attaggttgg tggaaca
//
LOCUS       DAOQVW010000007         7084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_11705_length_7084_cov_3.897425, whole genome shotgun sequence.
ACCESSION   DAOQVW010000007 DAOQVW010000000
VERSION     DAOQVW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11705_length_7084_cov_3.897425"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PKK85_00315"
     CDS             complement(<1..510)
                     /locus_tag="PKK85_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HNS24500.1"
                     /translation="MTEENQLIQVYRKLYQLYGPQGWWPLMDWEGKNPRKSGSSRGYH
                     PLNYSLPENSRQRFEVILGSILTQNTAWTSAEKALWNLKNLDAVHPQSLLQLETEILK
                     EAVRPAGFFNQKSVYLQNISRFFLELEDNVPSRKELLEVKGVGNETADSILLYAYHQP
                     QFVVDNYTKR"
     gene            585..1676
                     /gene="aroC"
                     /locus_tag="PKK85_00320"
     CDS             585..1676
                     /gene="aroC"
                     /locus_tag="PKK85_00320"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNS24501.1"
                     /translation="MSGNSTGIMFRVTTFGSSHGKALGAVIDGCPAGLDLTRMDIQGE
                     LDRRRPGASSITTSRGETDQVEILSGIFQGKTDGTPIAAVIYNEDADSSAYEAIKHRP
                     RPGHGDYTWTARYGVYDYRGGGRGSGRATVGHVIGGAVAKKLLKLLDIKVISHVTQIG
                     DIKASPVSHSRIEEYSTKNPVRCADPQAAPKMEEAILEAKAKGDSLGGIVEIIAFQVP
                     AGLGEPVFGKLDAELAQALMGIGSVKGVEVGLGFELARSTASATNDEYYLEKGQIKTT
                     TNTSGGIVGGISNGMPLVMRMAVKPTPSISLPQKTVDLEKKEEVEIKILGRHDPCICP
                     RITTVAESVVAMVLADHLLRTGLINPCHV"
     gene            complement(1697..1873)
                     /locus_tag="PKK85_00325"
     CDS             complement(1697..1873)
                     /locus_tag="PKK85_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24502.1"
                     /translation="MDSPDETLRICRAQCHCPHDCCRHYRWHPKLDSCGLRKCNMLGI
                     MVKCSTDPEKSMMD"
     gene            complement(2048..2121)
                     /locus_tag="PKK85_00330"
     tRNA            complement(2048..2121)
                     /locus_tag="PKK85_00330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2085..2087),aa:Thr,seq:cgt)
     gene            complement(2167..2967)
                     /locus_tag="PKK85_00335"
     CDS             complement(2167..2967)
                     /locus_tag="PKK85_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS24503.1"
                     /translation="MKLTFLGSGGGRFATITQRRMTGGIRLDGIDGKNLHLDPGPGAL
                     VRSYQFGLDPLKLDAVLVSHCHTDHYNDAEVLIEAMTRGMTRKRGTVVGSQSVINGYQ
                     QWGPSISKYHLGKSEVKALQPGEEYELGNISITGTRTQHGDPMTIGFKLEWNGFILSY
                     TSDTSYFPELKKEHYRADVLISSVIRPGGEKIRGHMCADEFEKLVEEVSPRLAVMTHL
                     GMKMIMDHPEEEAERITTRTGVKTLAAQDGMKLNLDCYLARQHTLDEF"
     gene            complement(2990..3913)
                     /locus_tag="PKK85_00340"
     CDS             complement(2990..3913)
                     /locus_tag="PKK85_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2121 domain-containing protein"
                     /protein_id="HNS24504.1"
                     /translation="MSLIISYISSKGCVMIGDKRRIGFLGDEEDREILEGKLYSGAIK
                     TQKELLQEASAMGISLKITDDAEKVRRIGDVLVGEVKYRSPSKTLRKRIYGTSHAYHL
                     VKLTGSNIDSVEQGGVSIVVFGNQITKKLANEIISQGWKSQTTLQEVGELFQRIMEEV
                     AHASPSVSAEYDKLMVHPALDKKAAHALLRSTIIEDVKELAKWRAELKDKMVTAAREM
                     EIASRIMTSGVVGTVTGVKNHEMEVKLGKGVLALDTNWNVRAQVDETVLMRVEDPSQV
                     KTGDVVVIRDENLCISRSGANLSCDVILCNE"
     gene            complement(4230..5096)
                     /locus_tag="PKK85_00345"
     CDS             complement(4230..5096)
                     /locus_tag="PKK85_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogen output domain 1-containing protein"
                     /protein_id="HNS24505.1"
                     /translation="MSKTRILVVEDEAIVGMGIKQKLEDLGHKVLDIVFTGEDAVKRA
                     LETKPDIILMDIVLKGDMDGIEAASKIREHLDVPIIYLTAYSDEDVLNRARITEPYGY
                     ILKPFKKSEVNANIEMALYKHAAEKKKSEVIKNQVMADFYDFILSAMPTCADKSDSEM
                     RSLLLKVFAKRLEDEMRPSFEKEMLDVIPKDVSDKLDEKFNFYLNWLSHFFEGFGILT
                     ITKIDGPEYLFEFKNCPWIEESSSQPVFCLNCQAMVNQSLQWTEIEGSVDRDQTIADG
                     GDRCIFRFKLSP"
     gene            complement(5093..6871)
                     /locus_tag="PKK85_00350"
     CDS             complement(5093..6871)
                     /locus_tag="PKK85_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS24506.1"
                     /translation="MDKKIKFLILEDVPLDAELAERELQREGLNISTLRVDNEEDYLR
                     ELQNFQPDVVLADHSLPRFDGVTALKLTREMGNQIPYIFVSGKIGEDFAVEMLKAGAT
                     DYVLKSNLSKLPHAVQRALKEFKEQMERKTAQIDLLESEKKYRTLFEKTKNPILVLDG
                     DHNFIQCNDAALSFMEMDRETLLNRNIEEFVGAEYKNIFSEDLGVLKNIVEVSFKINH
                     QVKILELSITPVNLKAGNIIFLSGRDLTKQKRFENALKEREEKYRLLVENQTDLIIKF
                     DLDDHLLFVSPSYCEVFGKKEEEILGENFKPLIHEDDREETARALEKLHQPPHSIYLE
                     QRLMTKNGWRWLLWAHQGILDDDGNVVAFVGVGRDITKRKLAENRIIESLREKELLLR
                     EVHHRVKNNLQIISTLLSLQSAQIKDESVINLYRESQNRIRSIALIHENLYQSEDLAS
                     INFSVYVKNLITDLIDSYGGDSDMIKTFLSIDNVTMGIETAIPCGLIINELVSNSLEH
                     GFSMDKCGEIELNLNETGKRRFTLTIRDDGDPFPPDFDFKNTESLGLQLINSLVNQLD
                     AQIELDRDKKEYKIVFNELKYKERIR"
     gene            complement(6880..>7084)
                     /locus_tag="PKK85_00355"
     CDS             complement(6880..>7084)
                     /locus_tag="PKK85_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS24507.1"
                     /translation="VLHRIKEHETTKKIPVVILTSSREDRDIVESYELGVNSYVSKPV
                     EFEEFIDAVSTLGLYWMLINKPP"
BASE COUNT         1758 a         1727 c         1546 g         2053 t
ORIGIN      
        1 tcttttggtg tagttatcca ccacgaattg gggttggtgg taggcataaa gtagtatgga
       61 atcggcggtt tcgttaccca cccctttaac ctccaacagt tccttccggc taggtacatt
      121 gtcttccagt tctaggaaaa atcggctgat attttgtagg taaaccgatt tctggttgaa
      181 gaacccggct ggccgcactg cctccttaag gatttcggtt tcaagctgga gcaaactctg
      241 gggatgcact gcatccagat ttttcagatt ccatagggcc ttctcagcag aggtccaggc
      301 agtgttttgg gtaagtatgg atcctagaat tacctcaaat ctctgccggg aattttcggg
      361 taaactgtaa ttaaggggat gataccccct tgaagaacca ctctttctag gatttttgcc
      421 ttcccagtcc atcaggggcc accagccctg aggaccgtac aattggtaga gcttacggta
      481 cacctggatt agctggtttt cttctgtcat gggggttcct catataatta atagcagggc
      541 ctatcatata ataatataag agattattag agttgaggat tcacatgtca ggaaatagta
      601 caggaatcat gttccgggtg accaccttcg gatccagcca tggaaaagca ttaggggcgg
      661 ttattgacgg ctgccccgct ggtttggacc ttaccaggat ggacatccag ggtgaactgg
      721 accgtagaag gccaggggcc agtagtatca ccacttcccg gggtgaaact gaccaggtgg
      781 aaattttatc tggaatattc cagggaaaaa cagatggcac ccccattgcc gcggtgattt
      841 ataatgaaga tgccgattca tctgcctacg aggctatcaa acataggccc cgtcctggac
      901 acggggatta cacttggacg gcccggtacg gtgtttacga ctatcggggg ggtggaaggg
      961 ggagtggccg ggccactgtt ggccacgtca tcggtggtgc ggtggctaaa aagctcctga
     1021 aactattaga tattaaagtc atatcccatg ttacccagat tggagatatc aaggctagcc
     1081 ctgtctctca cagtcgaatc gaggagtatt caactaaaaa tccagtaagg tgtgctgatc
     1141 cccaggctgc acctaaaatg gaagaagcca tactggaagc caaggctaag ggagattcac
     1201 tgggcggcat tgtggagata attgccttcc aggtaccagc tggactgggg gaaccggtct
     1261 tcggtaaact ggacgctgaa ctggcccagg ccctcatggg tattggttcg gttaagggtg
     1321 tggaggtggg tttaggattt gaactggccc ggtccactgc cagtgccacc aacgatgaat
     1381 attacctgga gaaaggccag atcaagacca ccaccaacac atccggagga attgtagggg
     1441 gaatttccaa tggaatgccc cttgtgatga ggatggcggt taaacccaca ccctccatca
     1501 gtttgcccca gaaaacggtg gacctggaga aaaaggaaga agtcgagata aaaatactgg
     1561 gccgccacga cccctgcatc tgtccaagga tcaccaccgt tgcagaatca gtggtggcca
     1621 tggtcctggc tgatcatctc ttaagaactg gcttaataaa tccttgtcac gtgtaacatg
     1681 cacttatatg gcatagttag tccatcattg atttctccgg gtcggtgctg cacttcacca
     1741 ttatccccag catgttacac ttccggaggc cacaggagtc tagtttaggg tgccagcgat
     1801 aatggcgaca acagtcatgg gggcagtggc actgggcccg gcagattctt aatgtttcat
     1861 caggactatc caaataacat ccccctctat ctcctgttac ccaacagtgc atggtacatc
     1921 cggtttatgg tatactgagt ggataggaac ttctttttaa tggtggtaga agtgcctgca
     1981 cttatccacc tgcctctgat aggagatgag acatcttacc cctatcaaat ccttagttaa
     2041 agaaaagagc cccggggggg atttgaaccc ccgaccacga gattacgagt cacgcgctct
     2101 accagactga gccaccgagg ccacaaatag tataggactt ttctaaattt aaatattcac
     2161 ctttacttaa aactcatcca gggtatgctg cctggccagg tagcagtcca ggttcagctt
     2221 catcccatcc tgggcagcca gggtcttaac tccagtgcgg gttgtgatcc tctctgcttc
     2281 ctcctcggga tgatccatga tcatcttcat ccccagatgg gtcataactg ccaggcgggg
     2341 tgaaacctcc tccaccagtt tttcaaattc atcggcacac atatggcccc ttatcttttc
     2401 cccacctgga cgtatgacgc tgctgataag tacatctgct ctgtagtgtt cttttttaag
     2461 ttccgggaag taggatgtgt cggaggtgta ggagaggatg aaaccattcc attcaagttt
     2521 gaaaccgatg gtcatgggat ctccgtgctg ggttctggtt ccggtaatgg agatgttgcc
     2581 cagttcatac tcttcgccgg gttgcagggc cttcacctcg gatttgccta gatggtactt
     2641 ggaaatggat ggtccccact gttgataacc atttatcaca ctctggctac ctacaactgt
     2701 gccccttttc cgggtcatcc ccctggtcat ggcttcgatg agcacctcgg cgtcattgta
     2761 gtgatcggtg tggcagtggg aaaccagcac tgcatccagc ttcaggggat ccaatccgaa
     2821 ctggtagctg cggaccagtg ctcctggtcc cgggtccagg tgcaagttct taccatcaat
     2881 tccatccaga cggatacccc cggtcatccg gcgctgtgta atggtggcga aacgtccacc
     2941 accacttcct agaaaggtta acttcatctt acaccacttg atacaaattt tattcattac
     3001 acaatataac atcacagctg agattagcgc ctgaacggct tatacacaaa ttttcatccc
     3061 taataaccac cacatcccct gttttcacct gggatggatc ttctaccctc atcaacactg
     3121 tttcatccac ctgggcccgc acgttccagt tggtatccag ggccagaacc cccttgccaa
     3181 gcttaacttc catttcatgg ttcttaacac cggtcacggt acccaccact ccactggtca
     3241 ttattcgaga ggcgatctcc atctcacggg cggcggtgac cattttatcc ttcaattctg
     3301 cacgccactt agccaactcc ttcacatcct caatgatggt gctgcgcaac agggcatggg
     3361 ctgctttctt gtccagtgca gggtgcacca ttaacttgtc atattctgca ctaactgatg
     3421 gactggcatg tgccacttcc tccatgatcc tctggaacag ttcacccact tcctggaggg
     3481 tggtttggga tttccacccc tgacttatga tctcatttgc cagcttcttg gttatctggt
     3541 ttccaaagac tacgatggaa accccaccct gctccacgct atcaatattt gacccagtaa
     3601 gtttcaccag atgataggca tgactggtcc catagatccg tttcctcagg gttttagatg
     3661 ggcttcgata tttcacctct cccaccagaa catcaccaat cctacgcacc ttctccgcat
     3721 catcagttat ttttaaagat atccccatgg cagatgcttc ctgtaaaagc tctttttggg
     3781 tttttattgc tccggagtaa agttttcctt ccaggatttc tctatcttcc tcatcaccta
     3841 gaaatccaat tctccgcttg tcaccaatca taacgcagcc tttactgctg atgtaactaa
     3901 taataagact catgacttca ccaaaaaaat tttataaaca cccactttag tgatggatta
     3961 gaatttgttt aaggatggga tatatagtat ttttgattta attatagggc tcccctgagc
     4021 tgggatgata tcattagttg tgaagcttag cagaggatca gaaaattaag caccacataa
     4081 acgattaggg aaaccgcaac aaatccagat aaagaaagac tcctgatccc ctcctgatcc
     4141 tgatactaag tgagattaat taatggaaac tctaagtttt ttttaggatt aaggacttgc
     4201 tacccagttt aagattactg gaaacaccct tagggcgata acttgaatcg gaagatgcat
     4261 ctatccccgc catcggctat ggtctggtca cgatccacag aaccctctat ttcagtccac
     4321 tgcaggctct ggttgaccat ggcctggcag ttaagacaga aaactggctg gctggatgat
     4381 tcctctatcc aggggcagtt tttaaattcg aacaggtatt cagggccgtc gatcttggtt
     4441 atggttagta taccaaatcc ttcgaaaaaa tgagacagcc aattcagata gaagttgaac
     4501 ttctcatcta acttatctga tacatcctta gggatgacat ctaacatctc tttctcgaag
     4561 ctgggccgca tttcatcttc caggcgtttg gcaaaaacct tcagtaagag ggaacgcatt
     4621 tctgagtctg atttatcagc gcaggtgggc atggcactca ggatgaaatc gtaaaagtca
     4681 gccatcacct gattttttat cacttcactc ttctttttct cagcggcatg tttgtaaagg
     4741 gccatctcta tattggcgtt tacttcactt ttcttgaatg gcttcaggat gtaaccatag
     4801 ggttcggtta tcctagcccg gttcagaaca tcctcatccg agtatgcagt taagtagata
     4861 atgggcacat cgagatgttc cctaatttta ctggccgctt caataccatc catatccccc
     4921 tttaatacaa tatccatcaa tattatatcc ggtttagtct ccagggctct tttcacagca
     4981 tcttcacctg taaacacgat gtccaggacc ttatggccca ggtcttccag tttttgtttg
     5041 atgcccatac ccactatggc ttcatcttcc accactaata tccgggtttt actcatcgga
     5101 tcctctcctt atatttaagt tcattgaaaa ctattttata ctctttctta tccctatcta
     5161 attctatctg ggcatctaac tggttaacca ggctgttaat cagttgtaaa cccaggctct
     5221 ccgtattttt aaaatcaaaa tcagggggga atggatctcc atcatccctg attgtgagtg
     5281 tgaatcttcg ttttccggtt tcattaagat tgagctctat ttccccacat ttatccatag
     5341 aaaatccatg ctccagtgag ttagaaacca gttcattgat tatgaggcca catgggatag
     5401 cggtttcaat tcccatggtg acgttgtcaa tgctgaggaa cgtttttatc atgtcggagt
     5461 ccccaccgta tgagtctata aggtcggtga taaggttttt tacgtacacc gagaagttga
     5521 tactggccag gtcttctgat tggtaaagat tttcatggat aagagcaatg gatcgtatgc
     5581 ggttttgact ttcacgataa aggtttataa cggattcatc cttgatctgt gctgattgca
     5641 aactcaggag ggtgcttatg atctgcaagt tgttcttgac cctgtgatga acttctcgca
     5701 gtagaagctc cttttctctt aaagattcaa taatacgatt ttcagctagt tttcgcttgg
     5761 taatatccct accaacacct acaaaggcaa ctacattgcc atcatcatct aatatgccct
     5821 gatgtgccca taacaaccag cgccatccat ttttagtcat taatcgctgc tccaagtata
     5881 tggagtgggg tggttggtgt agtttttcca gggccctggc agtttcttct cgatcatctt
     5941 catgaataag gggcttgaaa ttctctccca gaatttcttc ctctttttta ccaaaaactt
     6001 cgcagtagga gggacttaca aataagagat ggtcatccaa gtcaaactta attatcaagt
     6061 cagtctggtt ttcaaccagc agccggtatt tttcttctcg ctccttcagg gcgttctcaa
     6121 atcgtttctg cttggttagg tccctaccag ataagaagat gatatttccg gctttcaaat
     6181 taacaggggt gatggataac tccagtatct tcacttgatg gttaatctta aatgaaactt
     6241 ccacaatatt ttttaacacc cctaaatctt cactaaagat atttttatat tcagcaccaa
     6301 cgaattcttc aatgtttcgg tttagaagcg tttcacggtc catttccatg aaggacaggg
     6361 ctgcatcatt gcactgtata aagttatggt ctccatcaag taccagtata gggttttttg
     6421 tcttctcaaa aagggttctg tatttcttct cactttccag gaggtctatc tgggcagttt
     6481 ttctctccat ctgttctttg aactctttca gagcccgttg cactgcatgg ggtagtttgg
     6541 acaggttact ttttaggacg tagtcagtgg cacctgcctt gagcatctcc actgcaaagt
     6601 cttcaccaat tttcccactc acgaagatgt aggggatctg atttcccatt tcccgggtca
     6661 gcttcagtgc ggtaacccca tcaaatcggg gcagggaatg atcagccagc actacatcgg
     6721 gctggaaatt ctgaagttcc ctgagataat cttcctcatt atccacacgt aaagtagata
     6781 tgtttaagcc ttccctctgt aattcgcgct ctgctagttc agcatccagt ggaacgtctt
     6841 ctaggattag aaactttatt tttttatcca tatcatcctc taggggggtt tgttaatgag
     6901 catccagtac aatcccagcg tggaaaccgc gtcgatgaat tcttcaaact ccacgggttt
     6961 gctgacgtaa ctgtttaccc ccagttcata gctttccact atatcccggt cctcccggga
     7021 tgaggttaaa ataaccactg gtatcttctt ggtggtttca tgctccttta tccggtgaag
     7081 aacc
//
LOCUS       DAOQVW010000008         7069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_11733_length_7069_cov_3.858854, whole genome shotgun sequence.
ACCESSION   DAOQVW010000008 DAOQVW010000000
VERSION     DAOQVW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11733_length_7069_cov_3.858854"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKK85_00360"
     CDS             complement(<1..413)
                     /locus_tag="PKK85_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24508.1"
                     /translation="MLIAENYGSENFPISDFVSLNPETVYYWKVVAEDSNGGISEGAI
                     WSFRTVHLGNYPPSIPANPDPSDGAYNVPVTTSLSWTCNDLDNDPIVYDVYFGTSSNA
                     NDLSLIAENYGSENFPLSDLVSLDPETIYYWKVVA"
     gene            533..2002
                     /locus_tag="PKK85_00365"
     CDS             533..2002
                     /locus_tag="PKK85_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNS24509.1"
                     /translation="MTSGQTLERLTGPNHFVVSGNTFYRTSGQSTSGTEASWDSRYGT
                     AAGSIRFIGTFDTVTLSWNCIGTSNDDQLELVWELECPRADLTVDKYPNRNPSYYNVG
                     EEVVFTVTARNSGPSAATGVVVTDTLPVGLTFVSASDGGVWDANTRTVTWNVGNLAVD
                     GEFSALLTALINAGTQGQTLVNAVSAVNDQNPDAVTDTCDVTVNNAVLTVDKQSDRDP
                     ANYNAGEEVVFTVTARNSGPSAATGVVVTDTLPVGLTFVSASDGGVWDANTRTVTWNV
                     GNLAVGASFTPTVTATVTGDAAAKTLVNTATADNDQMDNPVSAVASFYVPSADLVLTK
                     TVDKSAPMVKDTVVFTLIVNNNGPDTAVDVTVDDKLPAGLTYVSHVANFGTYDPVTGL
                     WSISSLPNGASAVLTITAVVEESGRIVNQANVTALTWDPNLEGNTASAAMDVREQPKP
                     EPKPVHGKTVKMEKTGAPIGALLVAFFMLLAGMVLPRRK"
     gene            2169..2294
                     /locus_tag="PKK85_00370"
     CDS             2169..2294
                     /locus_tag="PKK85_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24510.1"
                     /translation="MEDDCGEGDEGPHQGEKQGDEGWKDTALVPGQILPHGELVG"
     gene            complement(2473..4446)
                     /locus_tag="PKK85_00375"
     CDS             complement(2473..4446)
                     /locus_tag="PKK85_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HNS24511.1"
                     /translation="MNESLKGLLVVLALGVVGLAFLWVPLPGGIPLFLLSYLLLFVLP
                     GFVLISYFDPEMSMVSRLVYGFVISLALLFGMFFVVALLELYSLVDYLPSFLLVLSIL
                     VALASYLGSGFRDKADIDQGQLTLFEAIERAQRVRDLVNHEVDVEPASGEPAISGVYK
                     AQVEEPVPIQEEDTAPKRIRMDVKPLQEEHLQNSFLEEEPEVEVSHPMWRDGLPQDGG
                     FIYWDLLATLFLLVACTGFLLLDPLKRPVYLTIFFLLGYALVVLLYPAPGRLALAKRV
                     GATILLGLLVLGVSYLLWIRGYLASLPLFYILAIFTLVALLVGGFRRRFASREEEALE
                     EDTHHEEMPEVIELWEELDEKVFEEEPVIIPDHVVQEEEHWDEDLEDIVPEVVALREI
                     LETNKEEVVTREEQSLDDVQEEDIEELIPEIVALKKMVLPDEEEEVSSGGSGEDVIVP
                     DHVFPVETSTLRTRETESKALREEPAGTSDAPLEEPIQQRELSTDKKQGLVLPWIGRE
                     KRPVHHVYENKPHHMTPQRIGSGYPLDLILVVIFTLLTVAFVLIPVLNQTPVRTVLGI
                     LLVLFLPGYSLIAALFPRRGDLDSVERVALSFGLSIAVTPLIGLVLNYTPWGIRLDPI
                     LVSLAGFTLPMCLISHLRRRSLSDDEKFIVAFG"
     gene            4514..4801
                     /locus_tag="PKK85_00380"
     CDS             4514..4801
                     /locus_tag="PKK85_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNS24512.1"
                     /translation="MENENMENRKITISSRVEVAQEVVSCDQAGEDPIFNMKDGVYYG
                     LDPVGASIWNQILKPAVLVDVRDQILHEYDVEEECQSDLLELVEQLVEVLG"
     gene            4894..5502
                     /locus_tag="PKK85_00385"
     CDS             4894..5502
                     /locus_tag="PKK85_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24513.1"
                     /translation="MNLIHAELCIKDGKNVIYYNKDLSQCYKNLKQNFTCVYLDEPVP
                     VKSRLINAILAISKDQTPTLSRFTIIELIKILVTELENRRLIIFFNHFDRLTPRSAHV
                     YQQLNSYKNIIFVCNFNQDITPDLYPFFKTFELMNKEEYRLASGENEINITYAAYALI
                     SGTCFFIYLKAASYMLIATMLIGGVWFALIIYRTLIYAGGRI"
     gene            5499..6131
                     /locus_tag="PKK85_00390"
     CDS             5499..6131
                     /locus_tag="PKK85_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNS24514.1"
                     /translation="MRSYTHIAAAILFYMILAFLLELGHFLLGLILAAWISVFPDLVD
                     RLTGKHRGIGHSLLWFIPFSSLFIFNVDIGAAILVGFLSHLFMDTFTVHGCPALYPIL
                     ETNFVCLAKKRRIKTGTKMDKAVFVFLIFLIVPAALFATGNHALIIGDEENSTAFATR
                     TSPTTSSPYTIKNDVTLNFRLDEATNKNVTVERINENMTTILVKDMDPGG"
     gene            6296..6697
                     /locus_tag="PKK85_00395"
     CDS             6296..6697
                     /locus_tag="PKK85_00395"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HNS24515.1"
                     /translation="MGIINSFITLDKEDKYLIIKTLILGYRIRFMTWIYSFPKLQEKI
                     NKMGDKKGLELGVYRIIWAVQATSPYILRSTCLTKAFTALVPLSLNDCSSTLRIGVKK
                     ESDFEVHAWLEMDGVVVMGESEREYAYLTDL"
BASE COUNT         1912 a         1679 c         1732 g         1746 t
ORIGIN      
        1 tctgcgacaa ctttccagta atagatcgtt tcgggatcga gagataccaa atctgaaagt
       61 ggaaaatttt cagagccgta attctctgca atcaatgaaa gatcattggc attgctagat
      121 gttccaaaat agacgtcata cactattgga tcattgtcca gatcattaca cgtccaggac
      181 agtgaagttg tcaccggcac attgtaagct ccatctgaag gatctggatt ggcgggaatc
      241 gaaggagggt agtttccaag atggacggtg cgaaaagacc atatagctcc ttctgaaatc
      301 cctccgttac tgtcttctgc gacaactttc cagtaataga ccgtttcagg attgagagat
      361 acgaaatctg aaatagggaa attttcagaa ccataattct ctgcaatcaa cattcggatc
      421 acgtggagga acaatcacct tcaacttcag tgccaacatc accaaccccg tccttcacat
      481 cgacagaatt ggaggagcgt ctggtggaca atcaacttcg gctaggttca cagtgacctc
      541 aggacagact ttagagagac taacagggcc caaccacttt gtggtatcag gcaacacctt
      601 ctaccgaacc tctggtcaat caaccagtgg tacagaggcc agttgggaca gccggtacgg
      661 tactgccgcg ggaagtatac gattcattgg aaccttcgac accgtcacct tatcctggaa
      721 ctgcatagga acatccaatg acgaccaact cgagttagtc tgggaattag aatgtccaag
      781 ggcagatcta acagtggaca agtacccgaa tcgcaacccc tcttattaca atgtgggtga
      841 agaggttgtc tttacggtta ctgctcggaa tagtgggcct agtgctgcta ctggtgttgt
      901 ggtgactgat actctgcctg ttggtttgac ttttgtcagt gccagtgatg gtggggtgtg
      961 ggatgctaac accaggactg ttacctggaa tgtgggtaac ctggctgttg atggtgaatt
     1021 cagtgccttg ctcactgctt tgatcaacgc tggtactcag ggtcagactt tggttaacgc
     1081 ggtttctgcg gttaatgacc agaatcctga tgcggttact gacacttgtg acgtgactgt
     1141 gaacaatgcg gttttgacgg ttgataagca gtcggatcgt gatcctgcca attacaatgc
     1201 gggtgaagag gttgtcttta cggttactgc tcggaatagt gggcctagtg ctgctactgg
     1261 tgttgtggtg actgatactc tgcctgttgg tttgactttt gtcagtgcca gtgatggtgg
     1321 ggtgtgggat gctaacacca ggactgttac ctggaatgtg ggtaacctgg ctgttggggc
     1381 tagttttact cctactgtga ctgctactgt gactggtgat gctgcggcta agaccctggt
     1441 gaatacggct actgctgata atgaccagat ggataatcct gtgtctgctg tggctagttt
     1501 ctatgtgccg tctgcggatt tggttctgac taagacggtg gataaatctg cgcctatggt
     1561 taaggatacc gtggtgttca ctttgattgt gaacaacaat gggcctgata ctgcggtgga
     1621 tgtgacggtt gatgataagc tgcctgctgg tttgacctac gtgtctcatg tggcgaactt
     1681 tggtacctat gatccggtta cgggtctgtg gagtatcagt agcttgccta atggtgctag
     1741 tgcggtttta accatcactg ctgtggtgga ggaatctgga cggattgtta accaggccaa
     1801 tgtcactgcc ttgacttggg atcctaacct ggaaggaaat actgctagtg cggctatgga
     1861 tgtccgggag caacctaaac cagagcctaa gccggttcat ggtaaaactg tgaagatgga
     1921 aaagactggt gcaccaatcg gtgccttgct tgttgccttc ttcatgctac tggccggtat
     1981 ggtactacca agacgcaaat agaaattaag gagaaaaaaa acacgcaccc actttctcca
     2041 cacccttttt ttttagccag taattttttt ttatgaatta gtaagaatca gtatagggaa
     2101 tctttttttc acggaattta aaaaaaaccc tatcactttc cctggcgcag gatcaggcgg
     2161 ctgatactgt ggaggatgat tgcggtgaag gagatgaagg tccccatcag ggtgaaaaac
     2221 agggtgatga aggctggaag gacactgccc ttgttcccgg ccagatactc ccccacggtg
     2281 agttggtggg gtagttattg gccttacaat ctggtcccag aatgtggagc tcggtgaact
     2341 ccccatcttt gggttttaag accacgaaac cacggtgcct atggccatta agatggagag
     2401 gatgagaatc acgatgagga tcttctcgct acgtgatgct agctggaagg atcttttaac
     2461 tttggtgaag aactacccaa atgctactat gaatttttca tcatccgata gggatcttct
     2521 tctaaggtgg gagatgaggc acatggggag ggtgaaaccc gccagactta ccagaatagg
     2581 atccagacgg atcccccagg gggtgtagtt caaaaccaac ccaataaggg gggtgacggc
     2641 gatggacaga ccaaaagaca gagctaccct ctcaacagag tccaaatcac ccctacgcgg
     2701 gaataaagca gcgattaatg aataaccggg tagaaatagc accaggagta tgcccagtac
     2761 ggttcggacc ggggtttggt tcaagaccgg gatgagtacg aatgccactg ttaagagggt
     2821 gaatatcacc accaggatga gatccagggg atagccggag cctatcctct ggggagtcat
     2881 gtggtggggc ttgttctcat agacatggtg aactggcctt ttttctcttc caatccaggg
     2941 gagcaccaac ccctgttttt tatcggtgga gagctctctt tgctggattg gttcttccag
     3001 gggtgcatca cttgtcccgg ctggttcttc acggagagcc ttagactctg tttccctagt
     3061 tctcagggtg gacgtttcca ccggaaaaac atgatcagga acgataacat cttcaccaga
     3121 ccctcctgat gatacctcct cctcttcatc tgggagcacc attttcttca gggcaacgat
     3181 ttccggaatc agctcctcta tatcctcctc ctggacatca tccagggact gctcctcccg
     3241 ggtaacaact tcctccttgt tagtttccaa tatttcccgg agggccacca cctcgggtac
     3301 gatgtcttcc aagtcttcat cccaatgttc ctcctcctgg acaacgtgat cagggatgat
     3361 aactggttct tcctcgaaaa ccttctcatc cagctcctcc cagagttcta tgacttccgg
     3421 catctcttca tgatgggtgt cttcttccaa tgcttcctcc tcccgggagg cgaacctgcg
     3481 ccggaagcca cccaccagta aagccaccag ggtgaagatg gccaagatgt agaagagtgg
     3541 cagggaggcc aagtagccac ggatccacag gaggtaggaa acccctaaga ccaggaggcc
     3601 caggaggatg gtggccccca ccctcttagc caaagccaac ctaccagggg cagggtagag
     3661 gagcaccacc agggcatagc ctaggaggaa gaagatggtc aggtagacgg ggcgcttcag
     3721 ggggtccagg aggaggaacc cggtgcaggc cacaaggaga aataaggtgg ctaagagatc
     3781 ccagtagatg aaacccccat cctgggggag cccatccctc cacatgggat gggaaacctc
     3841 tacttctggt tcctcctcca ggaagctgtt ctgcagatgt tcctcctgga ggggcttcac
     3901 atccatccgg atcctcttag gggccgtgtc ctcctcttgg atcggcactg gttcctccac
     3961 ctgggctttg tagacgccac tgatggctgg ttctccactg gctggctcca catcaacctc
     4021 gtggttaaca aggtcacgca ccctctgagc cctttcaatg gcctcaaaga gggttaactg
     4081 gccctgatct atatccgcct tatctctgaa ccctgatcct aggtaactgg ccagggccac
     4141 caggatactc aggaccagga ggaaactggg caggtaatcc accaggctgt ataactccag
     4201 gagtgccaca acaaagaaca tgccaaataa gagggccagg ctgatgacga aaccatagac
     4261 cagccgtgat accatgctca tctctggatc aaagtaggag atcaggacga agccaggcag
     4321 tacaaagagc agcagatagg ataagaggaa taatggtata ccaccaggga ggggcaccca
     4381 aagaaatgcc agccccacca cgcctagggc caggacaacc aggaggccct taagggattc
     4441 attcataaac aaatttttga gttcatatga tatataaggt tagaggctga aatgagaata
     4501 ggagtagtgt tagatggaga atgagaatat ggaaaacaga aaaatcacca tatcatcccg
     4561 ggtagaggtg gcccaggaag tggttagctg tgaccaggct ggcgaggacc ccatcttcaa
     4621 catgaaagac ggagtttact atggactgga ccctgtgggg gccagtatct ggaatcagat
     4681 cctgaaacca gccgtactgg tggatgtgcg tgaccagata ctccatgagt acgatgtgga
     4741 ggaggaatgc cagtctgacc tcctggaact ggtggagcaa ctggtggaag tactgggttg
     4801 aactggcagg cacctactaa tggatctgcc aatttgttgg attgacctag gtgtacttag
     4861 atttatatgt aacattgtat aataattaaa ggtatgaatt taatacatgc tgagttgtgt
     4921 attaaggatg gaaagaacgt tatttactac aataaagatc tttcccagtg ttacaaaaat
     4981 ttgaagcaaa atttcacatg cgtgtacctg gatgaaccag taccagttaa atcaaggctg
     5041 ataaatgcca ttttagctat atctaaggac caaacaccaa cactgagtcg ttttaccata
     5101 atcgagctca taaagatcct ggtaaccgag ttagaaaacc gtagattaat aatttttttc
     5161 aaccacttcg acaggctcac ccccagatca gcccacgtct accagcaact gaacagctac
     5221 aaaaacatca tattcgtctg caacttcaac caagacatca ccccggatct ctacccattt
     5281 tttaagacat tcgaactaat gaacaaggaa gaataccgcc tagccagtgg tgaaaacgaa
     5341 ataaacatca catatgcagc atacgccctg atcagcggga cctgtttttt catatacctc
     5401 aaagccgcct cctacatgct aatagcaacg atgctcattg ggggtgtctg gtttgcactc
     5461 atcatctacc ggacactgat ctacgctggg ggcaggatat gagaagctac acccacatcg
     5521 cagccgccat actcttctac atgatcttag cattcctcct agaactaggc cactttctcc
     5581 tgggcctcat actcgcagct tggatatcgg tgttcccgga tctggtggac cgcttaactg
     5641 gcaaacaccg gggaattgga cactcactac tatggttcat cccattctct tcactcttca
     5701 ttttcaacgt ggacatagga gccgccatcc tggtcggatt tctctcccac ctcttcatgg
     5761 acaccttcac agttcacggc tgcccagcac tctaccccat cctagaaacg aacttcgtgt
     5821 gcctggcaaa gaagagaagg ataaaaaccg gtaccaaaat ggataaagcg gtgttcgtgt
     5881 ttttaatatt cctgatagtc cccgcagccc tctttgccac tggcaaccat gccctgatca
     5941 ttggagatga agaaaacagc actgcctttg caaccaggac cagccccacc actagcagcc
     6001 cttacaccat aaaaaacgat gtcaccctga acttccgcct ggatgaggcc accaacaaga
     6061 acgtgaccgt ggagaggatc aacgaaaata tgaccaccat cctagtaaag gacatggacc
     6121 ccggtggctg aagaccagga accatgagcc cgaaatagta catggccaac taagttgcca
     6181 agttacacct gatggcgtcg aaggctttac acgtaagata aaccactgat ctaacacaga
     6241 tagtacagag atcaacggat accatcatag catggtaagt gaaagggttt tatcaatggg
     6301 catcatcaac agcttcatca ccctggacaa ggaggataag tacctgataa taaaaacctt
     6361 aatcctaggg taccgcatcc gcttcatgac ctggatctac tcctttccca agttgcagga
     6421 gaagataaat aagatggggg ataagaaagg cctggagttg ggtgtttata ggataatctg
     6481 ggcagtgcag gccaccagcc cctacatact aagatccacc tgcctaacca aggccttcac
     6541 agccctggta cccctatccc tgaatgactg ttcatccact ctgcggatcg gggtgaagaa
     6601 ggaatcagac ttcgaggtcc acgcctggct ggagatggat ggagtggtgg taatggggga
     6661 atcagaacgt gaatacgcgt atcttactga tttataacag aaaattcgat taaaaaagaa
     6721 taaaaaaggt aaaaatttcc tattttaact ttaacattat ctgggatatc ttcttttata
     6781 agttcattac ttcccgactg gaagaaatta ccacataaac aaaatttatc ctcaaaaaag
     6841 ctcgacggaa gtttttttca ctttaaatgg ttttaagctt gatctgaaga tctttatctg
     6901 caccttatct aaacataggt ttctatttat tattatttca gtgctggtcc tttatacgaa
     6961 ccgtaatacc aagccaggat actgccttcc tgaaaaatct gctagaacac aaaaaaaaga
     7021 aaaaaaggag aacccccata gtggaggggt ttctatcttc ttctgcccc
//
LOCUS       DAOQVW010000009         7051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_11764_length_7051_cov_3.138508, whole genome shotgun sequence.
ACCESSION   DAOQVW010000009 DAOQVW010000000
VERSION     DAOQVW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11764_length_7051_cov_3.138508"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKK85_00400"
     CDS             complement(<1..741)
                     /locus_tag="PKK85_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNS24516.1"
                     /translation="MMQRVTLPAKAIPKKWYNLAADLPVEFPEYSQPEDGNQLENLPK
                     IFSSGVLKQEMSQKRYIKIPKKIRKIYKKVGRPSPLYRAKGLEKYLDTPAKIFYKRED
                     MSPTGSHKFNTAIAQAYYAKKDKAKMLTTETGAGQWGSALSLACNMMNIGCRVYMVRV
                     SFQQKPYRKTIMQLYNGEILPSPSDKTEFGRKILSEEPDHPGTLGIAISEAIEDALKD
                     DKIYYSLGSVLNHVMLHQTVIGQETKKQL"
     gene            860..1888
                     /locus_tag="PKK85_00405"
     CDS             860..1888
                     /locus_tag="PKK85_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HNS24517.1"
                     /translation="MKILAIDVGTGTQDIMFYDSEEPIENAIKLVLPSPTRIIANKIF
                     KHHHDIFLSGETMGGGPVTNAIKRHLEKGYRVVMTESAARTVRDDLDKVRAMGIEIVD
                     DGEQHPEIAEIRMGDVDLEVLKSALQFYGVTLEFDWLGVAVQDHGNSEGRGDRNFRFQ
                     KIREKMNSPSLPEEFAFHAQVPEYFTRMQGVLRILKGYHPTIMDTKFAALCGCTCDPH
                     VEAMEQFVAMDVGNGHTLAASFKKGRICGVFEHHTHMLTSSKMEQLVDRLVTGEITHE
                     EVHEDGGHGAWVVESIRKFDRVVVTGPQRKIINKTGFEVYNAAPAGDVMMVGPVGLIK
                     AVMSKRGQ"
     gene            1892..2539
                     /locus_tag="PKK85_00410"
     CDS             1892..2539
                     /locus_tag="PKK85_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNS24518.1"
                     /translation="MIIDTHIHSTYSPDSSSAVVDIVKYSRKIGLDAVAIADHDTMKG
                     SKEALNRFKGLKDFVIIPAMEISTNKGHIVALGIKDEISPGLDPDETVDLIRDQGGIA
                     VAAHPFVRYREGIFSRISSLDVDALETLNSRYIFGYSNWKARKMAEENNIPQLGASDA
                     HFLKAIGSCVTQLEADFDVESILETILSGKTNVYGDRTPLPLIIKEVINKKIKKV"
     gene            2576..3358
                     /locus_tag="PKK85_00415"
     CDS             2576..3358
                     /locus_tag="PKK85_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HNS24519.1"
                     /translation="MILEFIEENFLYLHPGYTLLNTIVFGIILGLAILIIIRMFKWLQ
                     KDPGELLLALVPFIFFGSSARALVDNGLYPLTLLLVTPGLYVLTGLITITALLLSVFM
                     EKKMGWDYRYTLFSLGSFMCLFNLVMIEHINPVPLLQILGAWALISAPFIFLRNKWWL
                     LKDKFNLSVLIAHLLDASTTFIAVDFYGYGEQHVLPNFLTQMVDTAVVMFPLKITVIL
                     TVLYIIDTSVEDKTTRNMLKLAIFVLGLAPGLRNFLSLIMGS"
     gene            3426..4949
                     /locus_tag="PKK85_00420"
     CDS             3426..4949
                     /locus_tag="PKK85_00420"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNS24520.1"
                     /translation="MYMDMVKAKMSLPERVKIFDTTLRDGEQTPGVAITPKEKLRIAK
                     RLDVLGVDTIEVGFPAASPGEQQAAREIKELGLKCQICGLARVLPEDLDAAISSDVDY
                     IHTFIGTSPLHRGYKLKMSKEDILQKAVEAVEYIKDHGIVAEFSAEDATRTEFDYLLE
                     IYQAVESAGADYINVPDTVGVMVPSAMNYLVGQLKNELAAPISVHCHDDFGLAVANSL
                     AAVEAGAEQVHATINGIGERAGNTSLEEVVMALIINYDVKININTKLLVDTSQMVSRI
                     TGVRIPPNKAIVGDNAFAHEAGIHVHGVLAKAETYEPLMPEVVGHTRRIVLGKHTGAR
                     ALWSKLDDYGIEMNEDQFCQVFDQVKKLGDKGKMVTDADLKALAETVLGRAQKETVKL
                     EGLSVLTGDNVLPTATIKLNIDGEVKTAAKTGVGPVDAAINAIQSLVSDTTDIELKEY
                     NIEAITGGTNALAEVFVIMADKEGNQATGRSTVEDVVMASVEAVLDAINKILMVKLI"
     gene            5054..5803
                     /locus_tag="PKK85_00425"
     CDS             5054..5803
                     /locus_tag="PKK85_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS24521.1"
                     /translation="MSGAKILVVEDERITAEDLKAGLEFDGYQVPAICSSGEDAVQKA
                     GKIKPDLVLMDIRLEGEMDGIEAAGEIRRLYDIPVIYLTAYSDEKTVERAKKTEPSGF
                     LVKGQGLINKPFEDTEIRAAIEITLYRHHMEKEHHQLFSAMLRNVSEAVISTDPSKKI
                     RFINNAAEKIIGRSKDEVLGKNLDDVFFRFNPIIEEETSLEDALSPGETPSYISPNGS
                     EVLLEGTVTPLNDNKGEVNGMVIIFRPQNGP"
     gene            5855..6604
                     /locus_tag="PKK85_00430"
     CDS             5855..6604
                     /locus_tag="PKK85_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS24522.1"
                     /translation="MAAAKILVVEDERITGEDIKMGLESAGYIVPSIVSSGEEALQEV
                     EKFHPDLVLMDIRLNGEMDGIEAAENIRSRFKIPIIYLTAFADENTLKRAKETQPSGF
                     IIKKPFGFLHKPFDDNELQTAIEITLKHHELEKKLSSPDQWIQKMLSNVSDAVISTDP
                     HGRINFMNTSAEDITGMKEQEVLDMKLHDLFISLANDDYEGDLEIGTPQELILMTRDG
                     SKLVVNGNITSIEGVDGKIEGYVTIFRVKQS"
     gene            6768..7043
                     /gene="albA"
                     /locus_tag="PKK85_00435"
     CDS             6768..7043
                     /gene="albA"
                     /locus_tag="PKK85_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048061063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HNS24523.1"
                     /translation="MSEENIVYIGNKPVMNYVLAVVTQMNGGVSEVILKARGRAISRA
                     VDVAEIVRNRFITDATVDNIDISTEEIMSNEGSSTNVSAIEISLTKE"
BASE COUNT         2137 a         1362 c         1634 g         1918 t
ORIGIN      
        1 cagttgcttc ttggtttcct ggccgataac agtttggtgt agcataacgt ggtttaggac
       61 acttcctagg gaataatata ttttatcatc ctttaaagcg tcttcgatgg cctctgagat
      121 ggcaatacct agagttccag ggtggtcagg ttcttcactt aatatttttc tgccgaactc
      181 tgttttatca ctgggggatg gcagtatttc cccgttgtag agctgcataa ttgttttacg
      241 gtacggtttt tgttggaagg atacccgaac catgtacacc cggcagccga tgttcatcat
      301 gttacacgct agtgatagtg ctgaacccca ttgtccggca ccagtttcgg tggtgagcat
      361 tttagctttg tctttttttg cataatatgc ttgagctata gcggtgttga acttatgact
      421 cccggtggga gacatgtctt cccttttgta gaagattttg gccggggtgt ccaggtattt
      481 ctccagaccc tttgcacgat aaagtgggct gggccgccct actttcttgt aaatcttcct
      541 tatcttctta ggtatcttga tatagcgttt ttgtgacatt tcctgtttca aaacgccact
      601 ggaaaatatt ttgggcagat tttccaactg atttccatct tcaggttgtg aatattcagg
      661 aaattctacg ggtaaatctg cggccaagtt gtaccatttt ttaggaattg ctttagccgg
      721 taaagttact ctctgcatta tttatcacct gcattcgtct ggctacacta tcttcaacgt
      781 actatttaaa tctttgttgt ataattttgt acattaattt gcattaatta taaacagtta
      841 agaacataag atactctaca tgaagatttt ggccattgac gtgggaactg gtacacagga
      901 tatcatgttc tatgactctg aagaacccat tgaaaacgca ataaagctgg ttctaccatc
      961 tcctactcgc ataatagcaa ataaaatatt taagcatcac cacgacattt ttctgagtgg
     1021 agagaccatg ggcggaggtc cagtgaccaa tgccattaaa agacacctgg aaaagggcta
     1081 ccgggtggtt atgactgaaa gtgccgcacg tactgtccgt gatgatttag acaaagtcag
     1141 ggccatggga attgagatcg ttgatgatgg ggaacaacat cctgaaatag cagagataag
     1201 gatgggtgat gtggaccttg aggtgctgaa gtctgcatta caattttacg gcgtaacatt
     1261 ggagtttgat tggttggggg tggcggtaca ggaccatggt aactctgaag gcaggggtga
     1321 tcgtaatttc cgcttccaga agatccggga gaaaatgaat tcaccttcac tccccgaaga
     1381 attcgctttc catgcccagg ttcccgaata ctttacccgt atgcagggag ttttacgtat
     1441 cttgaaaggc tatcacccca cgataatgga taccaaattt gcagctctat gtggatgtac
     1501 ttgtgacccc cacgtggagg ccatggaaca gttcgtggcc atggacgttg gaaatggtca
     1561 cacactggcc gcatccttta agaaaggtag gatatgtgga gtttttgagc atcacaccca
     1621 catgttgact tcatctaaga tggaacaatt agttgacagg ttggtaacag gagaaattac
     1681 ccatgaagag gtccatgaag atggtgggca tggggcctgg gtggtggaat ccattagaaa
     1741 attcgaccga gtggtggtaa ctggtcctca gaggaaaata ataaacaaaa caggttttga
     1801 agtttacaac gctgctcctg ccggggatgt tatgatggtg ggcccagtcg gcctgattaa
     1861 agccgtaatg tccaagaggg gtcagtagaa catgatcatt gatacacaca tacacagcac
     1921 ttactcgcca gattccagtt ccgcggttgt agatattgta aaatacagtc gcaagattgg
     1981 cctggatgca gtggccatcg ctgaccatga cactatgaag ggatcaaaag aggctttaaa
     2041 tagatttaag ggcttaaaag attttgtaat aattcctgcc atggaaatca gcaccaataa
     2101 gggccatatt gtggcgttag ggataaaaga tgagatcagc cctggtttgg atcctgatga
     2161 aacagtggat ctgatcaggg atcagggggg gattgccgta gcagctcacc cctttgtacg
     2221 ttaccgtgaa ggaattttca gccgcattag cagtttagat gtggatgcat tggaaaccct
     2281 taactcacgg tacatttttg gctattcaaa ctggaaggca cgtaaaatgg cagaggaaaa
     2341 taacattccc caattggggg ctagcgatgc acattttttg aaagctattg gcagttgtgt
     2401 gactcaatta gaggcagatt ttgatgtgga aagtatattg gagaccatat tatctgggaa
     2461 gaccaatgtt tatggcgaca gaacacctct tcctcttata ataaaggaag tcataaataa
     2521 gaagattaaa aaagtttgaa aaccaaataa agaattttac tcttaaataa ctgccatgat
     2581 acttgaattc atagaggaga acttcctata ccttcatcct ggctacaccc tgcttaacac
     2641 cattgttttt ggaattatcc tggggttggc tattttgatc attatccgca tgttcaaatg
     2701 gctccaaaaa gatccggggg aactgttact ggccctggtg ccatttatct tctttggctc
     2761 cagtgcccgg gccctggtgg ataatggttt gtatccccta accttgctcc tggttactcc
     2821 gggattatac gttttaaccg gattgataac cattaccgcc cttttattat cagtatttat
     2881 ggaaaagaag atgggctggg attacaggta tactctcttt agcctaggat cttttatgtg
     2941 ccttttcaac ctagtaatga tagaacacat aaacccggtt cctctacttc agatcctggg
     3001 ggcatgggct ctgatatcag caccattcat atttctgcgg aataaatggt ggttgttaaa
     3061 agataaattc aatttaagtg tacttattgc tcaccttttg gatgccagta ccactttcat
     3121 tgcagtggac ttctatggct acggagaaca gcatgttctc cctaatttcc ttactcaaat
     3181 ggttgacacc gccgtagtga tgtttccact aaaaataaca gttatattaa cggttttata
     3241 cattatagat accagtgtgg aggataaaac tacccggaac atgcttaaac tggccatatt
     3301 tgttttggga ttggctcctg gacttcggaa ctttctgagt cttatcatgg gtagttgatg
     3361 atcccaattt atttatgaaa caccacccac cataatatta acttaaaaaa agttgaggtc
     3421 ttcccatgta catggatatg gtgaaagcaa agatgagctt accagagagg gttaaaatat
     3481 ttgataccac acttcgcgat ggtgaacaaa ccccaggggt agccatcaca cccaaagaaa
     3541 agctaagaat agccaaacga ctggatgttc tgggagtcga taccatagag gtgggattcc
     3601 cggctgcttc accaggagaa cagcaagcag ccagagaaat aaaggaatta ggcctgaaat
     3661 gccagatttg tggcctagcc cgagttttac ccgaagatct ggatgctgcc atcagtagtg
     3721 acgtggatta catacacacc ttcattggca cttctcccct ccaccggggg tacaagttga
     3781 aaatgagtaa ggaagacatt ctacaaaaag cggtggaagc agtggaatat ataaaggatc
     3841 atggaattgt ggccgaattt tccgctgaag atgccactcg taccgaattt gattatttgc
     3901 tggagatata ccaggctgta gaatcagctg gggcagatta tattaacgta ccagataccg
     3961 taggcgtgat ggtcccatcc gccatgaact acctggtggg ccaactaaaa aatgagctgg
     4021 cagcacccat cagtgtacac tgtcatgatg actttggttt ggccgtggct aactccctgg
     4081 ctgcggtgga agcaggggca gaacaagtac acgccaccat caatggaatc ggtgaacggg
     4141 ctggtaacac ctccctagaa gaagtggtta tggccttaat catcaattat gatgtaaaaa
     4201 ttaatataaa cactaaatta ttggtggaca cttcccagat ggtgtccaga ataactggag
     4261 tcaggatacc tcccaacaaa gccatagtag gagacaacgc atttgcccat gaagccggca
     4321 ttcatgtcca cggggtatta gccaaagccg aaacctacga accactcatg cctgaggtag
     4381 taggccacac ccggcgcata gtactgggca aacacaccgg ggcccgagcc ctgtggtcca
     4441 aactggatga ctatggtatt gaaatgaatg aggatcagtt ctgccaagtt tttgaccaag
     4501 taaagaaact gggtgacaag ggtaaaatgg tcacagatgc agatttaaaa gctctggcag
     4561 aaacagtgct gggtagagct caaaaagaaa cagttaaact ggaagggctt tctgtactca
     4621 ctggagacaa cgtactgccc acggccacca tcaaactgaa catagatggt gaagtcaaaa
     4681 ctgctgcaaa aacaggtgtg ggtccggttg atgctgccat taatgctata caaagtctgg
     4741 ttagtgacac caccgatata gaacttaagg aatataatat tgaggcaata acaggtggaa
     4801 ccaatgccct ggctgaggtg tttgtgatca tggcagacaa agagggtaac caagccacag
     4861 gcagatccac agtagaagac gtagtaatgg ccagtgtaga agcggtattg gacgccataa
     4921 ataaaatatt aatggtaaaa ttgatctaaa gacttttaac ctaaaaatga cataaaatgg
     4981 ttgattgaat ggtttatata aaaggtggac ttaaatctac aacagacgat aaagaaactg
     5041 gaggtaggac caagtgagcg gtgcgaaaat tcttgtggtg gaggatgaac gaatcaccgc
     5101 tgaagatctt aaggccggat tggaatttga tggttaccaa gtcccggcta tttgttcttc
     5161 tggcgaagat gcagttcaaa aggcgggaaa aatcaagcct gatctggttt tgatggatat
     5221 cagactggaa ggtgaaatgg atggtataga agcagccggt gagatcaggc gactttacga
     5281 tataccagta atttatctga ctgcttattc tgatgaaaag actgtggaaa gagctaaaaa
     5341 gactgaacca tctggttttt tagtaaaagg ccagggactt attaacaagc cctttgaaga
     5401 tacagaaatc cgtgctgcta ttgaaattac cttgtaccgc catcatatgg aaaaagaaca
     5461 ccaccagtta ttttcagcta tgctccgcaa cgtcagtgag gctgtaatat ccactgatcc
     5521 ctctaaaaaa attagattca tcaacaacgc tgctgaaaaa atcattggac gctcaaagga
     5581 cgaagtacta ggcaaaaact tggatgacgt gtttttccga tttaacccta taattgaaga
     5641 ggaaacaagc ctagaagatg cactttctcc tggtgaaact ccgtcctata tatctcccaa
     5701 tggatcggag gtgcttttag aaggcacagt cacaccatta aatgacaata aaggggaagt
     5761 gaatggtatg gttatcatat tccggcctca aaatggccct taatcataat tatcgatcta
     5821 tactctttga accttaacta agaggggaat ggacatggca gctgcaaaga tcttggttgt
     5881 cgaagatgaa cggatcacag gagaagacat taaaatgggc ctggaaagtg caggttacat
     5941 cgttccatca attgtttctt cgggtgaaga ggcactacag gaggttgaga aattccatcc
     6001 ggacttggtt ctcatggaca taaggcttaa tggcgaaatg gatggcatag aagctgcgga
     6061 gaatataagg tcccgattta aaattccaat aatatattta actgcttttg ctgatgaaaa
     6121 tactttaaaa cgggctaaag agactcaacc atctggattc atcataaaaa aaccattcgg
     6181 atttttacat aaaccattcg atgataacga gctacaaacc gccattgaga tcactcttaa
     6241 acatcatgag ttggaaaaaa aattatctag tcctgatcaa tggatacaaa aaatgttgag
     6301 caacgtaagt gacgctgtta tctccactga tccccatggc cggattaatt tcatgaacac
     6361 atcggctgaa gatataactg gcatgaagga acaagaagtt ctggatatga agttgcatga
     6421 tctgttcatc tcccttgcaa atgatgatta tgaaggtgat cttgaaatag ggacacctca
     6481 agaactcatc ttgatgacca gagatggctc aaaattagtt gtaaatggaa atataacttc
     6541 tatagagggt gttgatggaa aaatagaggg atacgtgact atattccgtg ttaaacagtc
     6601 ttagtttttt gaaaaatctt ttaaaaaaaa catcgtaaat ttgtcaacca ttaaaactca
     6661 taaatgctaa aaattctgta aatacctagt gacattcttg aaaataaata tgtaatgtat
     6721 aataaggata aagattatta ccattactat cattggaggt taaagcgatg tcagaggaaa
     6781 atatagtgta cattggaaac aagccggtaa tgaactatgt cttagctgtg gtgactcaga
     6841 tgaatggcgg cgtctctgaa gttattctaa aggcaagagg aagagcaata agcagggcag
     6901 ttgacgttgc tgagattgtg aggaaccgat tcattaccga tgccacggtg gacaacatcg
     6961 atataagcac cgaggagatc atgagtaatg agggatcttc caccaacgta tccgcaatag
     7021 aaatatcttt aaccaaggaa tagtcagtga a
//
LOCUS       DAOQVW010000010         6917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_12067_length_6917_cov_3.535995, whole genome shotgun sequence.
ACCESSION   DAOQVW010000010 DAOQVW010000000
VERSION     DAOQVW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12067_length_6917_cov_3.535995"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3574
                     /locus_tag="PKK85_00440"
     CDS             <1..3574
                     /locus_tag="PKK85_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HNS24524.1"
                     /translation="RPPQNRQELEDALQEARIIARAPPVERELLENPAWNSATPGAKR
                     IIRELREYRNQYKLTHRFKEEVLDLDLKDRLESFKSVSAKRLKFLSGDYKKVKHSVLQ
                     LYSEDAPQDDQEIRRDLEGLIRLQDLRRSIRQSRDTAQSLFGQAWQGERTDSQFICDL
                     ADWLIRFRKLLLAGELSQEALLKFEKGPHPRIKTSISQLQEDLDTVTNLAGHLQGFIP
                     ISMDELHFQKLKLDAAASKSRVDGYFQLREKINELYREEAPEDTEEVIKDLHEVLASE
                     KTKKKLREKDPEATQLFGALWQQEDTDLEELQQRLQFIIRIHQLLDEEKISPRTLELL
                     SQERDPERISHIIQRVHSSYQELLRIWKEVNQYLELDCNQVFPGGFDYTPFTSIRSQL
                     SLYREEVPRLLTWSQFLSQTRELPPLAQPLLSLIKEDLLDGEDLLPCLMGDYADSMLR
                     QAFLEDPALQNFVGDLHQNKIRQFVELDQELLMLNRERIAHHLSETRPNVYTNLTPTS
                     ELGILLSELNRKRRHMPIRKLLLAAGGLIQTIKPCFMMSPLSIAQFLDPHGVPNLNFD
                     VVIFDEASQVKPEDALGALLRGRQLVVMGDTKQLPPTTFFDIIIHPLGEEDYELDSLM
                     DMESILHLCKRSFPIKMLRWHYRSRHESLIAVSNQEFYNNHLLVYPSPSHSSPELGLM
                     YVPLDRDIATYDRGRSSINLEEAKAVVQAAFDHYQTYGDGKSLGIGTFNVNQQQAILE
                     VLELELKRHPRAQKFHKHFFGEWEEKFFVKNLETIQGDERDVIFVSVGYGFDEEGKLT
                     HNFGPLNKDGGERRLNVLVTRAKEKCVVFANFHSGDLKNITEGSAFGLRALKTFLEYA
                     EHRNLESLDSHTTEASSPLESSIRSYLEEEGLEVHQQVGCAGYRLDMAVVDPEDPDRY
                     LLGIECDGAMYHSAPVARDRDRLRQQILEGLGWNIYRIWSTDWYRNREESLNRLYMAI
                     QEAREGKKKPEVELIEEETLVEEEAPPAPVVVSLEDMIPAYQLCKDLELSKDKPLHEK
                     LPQELAPAMRRVVDTEGPLHLSELYKRIRSHWGLKRSGRRIKETLDASLQLALDKGLF
                     TIQDDFLFSRTGEIRIRRRGEDIPARIELISDEEIKKAVLLVIETQHATYPEELVTQV
                     ARLFGFKSTRQATRDRVNHILRGYLEQGELVAMPNGMINFPRKD"
     gene            3603..5915
                     /locus_tag="PKK85_00445"
     CDS             3603..5915
                     /locus_tag="PKK85_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS24525.1"
                     /translation="MVENVLERLTNDPRFQDKVEHLEELEAQEAQYTPVDDLPPNIED
                     YLRREKIKLYQHQAQAIQSVREGENILITTPTASGKTLAFNLPVLEKLSLDPQATALY
                     IYPAKALSNDQLRVLRGLEKACDLELDPAIYDGDTPRDIRPDIRRHSRVGITNPYQLH
                     LILAWHHQWERFYSQLKFVVIDEAHQYRGVFGSNVAFLIRRLRRICNYYGSNPQFILS
                     SATLANPEEFSQKLVGQSFQLIDQDSSPRGRKHFLFYNPHLTPGASTHQETTHLFLFF
                     IRQGLQTLCFTLSRRMAELIARWSLQELQDTDPELAGQVTAYRAGYLAPERRKIEYGL
                     KTGALLGVTTTNALELGIDIGSLDAVIISGYPGTMISTWQQAGRSGRKNNDSLVVLVA
                     FEGPLDQYFMKNPQVFFDRHHENAIIDLENVHITFNHLLCAMSELPLSREEITSCFQT
                     SPGFMGRLIRSHQVRESQDRFTYQGKGSPAFQVGLHHISRDEFQVYHDGYLLETLDRP
                     HAYLEAHHGAVLINKGETYLVEEFNLQKQEVRVRKRNLDYHTQVLKEVDVRVLEWIHH
                     KQIGQFRVHYGEVEVTEHYHRYKRISQGKTVATHPLDLPPLHFRTKGLWFTLPGDVED
                     QINHYISGQEVYEGGLHGTEHALIAMFPLKVLCDRFDIGGLSTPHHPDTRGATIFIYD
                     AFQGGIGLAEKAVELMEELVELTYDMVRGCQCRDGCPSCIYSPKCGNDNQPLHKRSTQ
                     YILSRILKLMGKVDEADIHLEGATAHNGSE"
     gene            6443..>6917
                     /locus_tag="PKK85_00450"
     CDS             6443..>6917
                     /locus_tag="PKK85_00450"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNS24526.1"
                     /translation="MEEVNRLYQQGYIEKINPSPLKSQLSLKEARIWLKEARLNCNSG
                     GYRSSRIACYMAFFHAARSVLQRDGYQEDKPEYLALYLEKYNREGLLEEEWTQLLQWI
                     MNLHHQDLYHFQAFPSPEEADEAIEQGTDFVERISILLDETNYAKKAFLKQNAVQR"
BASE COUNT         1771 a         1959 c         1834 g         1353 t
ORIGIN      
        1 ccggcccccc cagaacagac aggagttaga agatgccctg caggaagccc ggatcatagc
       61 cagggccccc ccagtggaga gggagttgtt ggagaatccg gcttggaaca gtgccactcc
      121 cggggctaaa cgcatcatcc gggagctgcg ggagtaccgg aaccagtaca aactcaccca
      181 ccgcttcaag gaagaggtgc tggacctgga cctgaaggac cggctggaga gcttcaaaag
      241 tgtatcagcc aagcgcctga agtttctaag cggagactac aagaaggtta agcacagtgt
      301 actgcaactc tacagtgaag atgcacccca ggatgaccag gagatccgcc gggacctgga
      361 gggcctcatc aggctccagg acctgcgccg atccatccgc caatccaggg acactgccca
      421 gtccctcttt ggccaggcct ggcaggggga gcgcaccgac tcccagttca tctgtgactt
      481 agctgattgg cttatacgct ttcgcaaact acttttagca ggggaactaa gccaagaggc
      541 actgttgaaa ttcgagaagg gaccccaccc ccggatcaag accagcatct cccagttaca
      601 ggaagacctg gacaccgtca ccaacctggc agggcacctg cagggattca tacccatctc
      661 catggatgaa ctccacttcc agaagttaaa actagatgca gcagcctcta agagccgggt
      721 ggatggttac ttccagctac gggagaagat caatgaactg taccgggaag aagcaccgga
      781 ggatacggag gaagtcatca aggacttgca tgaggtgctg gcctctgaaa agaccaagaa
      841 gaagctaagg gaaaaggacc ctgaagccac ccaattattc ggtgccctgt ggcagcagga
      901 agacacagac ttggaagaac tgcagcaaag gctacagttc atcatccgca tccaccagtt
      961 actggatgag gagaaaatat ccccccgcac cctggagctc ttgagtcagg agcgggatcc
     1021 agagaggatc agccacatca tccagagggt gcactcctcc taccaggaac ttttgaggat
     1081 ctggaaggag gtgaaccagt acctggaact ggactgcaac caggtgttcc cagggggctt
     1141 tgactacacc cccttcactt ctataaggtc ccagctaagt ctctaccgtg aggaagttcc
     1201 ccggctttta acctggtccc agttcttatc ccagacccgg gaattaccac ccctggccca
     1261 gcccctcctc tcccttataa aggaggatct tctggacgga gaggatctct taccctgcct
     1321 gatgggggac tatgcagaca gcatgctccg acaggctttc ctggaagacc cggcactgca
     1381 gaacttcgtg ggagacctgc accagaacaa gatacgccag ttcgtggaac tggaccagga
     1441 acttttgatg cttaaccggg aacgcatcgc ccaccacctc agcgaaaccc gtcccaatgt
     1501 ttacactaac ctcaccccca catccgagtt gggcatactc ctatcagagc tcaaccgtaa
     1561 aaggaggcac atgcccatcc gtaaattgct cctggcagca gggggtttga tacagaccat
     1621 caagccctgc ttcatgatga gtcccctcag cattgcccag ttcctggacc cccatggggt
     1681 gcctaacctg aacttcgacg tggtaatctt cgacgaggcc agccaggtta aaccggaaga
     1741 cgcactagga gccctcttaa gggggcggca gctggttgtg atgggagaca ccaagcaact
     1801 accaccaacc accttcttcg acatcataat ccaccccctg ggtgaggagg actatgaact
     1861 ggactccctc atggatatgg agagcatact ccacctctgc aagcggagtt tccccattaa
     1921 aatgctgcgc tggcattacc ggagccggca cgagtccctc atagcagttt ccaatcagga
     1981 attttataac aaccacctcc tggtttatcc ctcaccatcc cacagctccc cagaactggg
     2041 cctgatgtac gtgccccttg acagggatat cgccacctac gaccggggac gtagctccat
     2101 caacctggag gaggccaaag ccgtggtgca agcggccttc gaccattacc agacctacgg
     2161 tgatgggaag agtctgggta tcggtacctt caacgttaac cagcagcagg ccatactgga
     2221 ggtcctggag ttggagctca aacgccaccc ccgggcccag aaattccata aacatttctt
     2281 cggggagtgg gaggagaaat tcttcgtcaa gaacctggaa accatccagg gagatgaaag
     2341 ggatgtgatc ttcgtcagtg tgggctacgg ctttgatgag gagggaaagt taacccacaa
     2401 cttcggacca ctcaacaagg atggtggaga acgccggttg aacgtgctgg tcacccgggc
     2461 caaggagaaa tgcgtggttt tcgccaactt ccactcgggg gatcttaaaa acataactga
     2521 aggatcagcc tttggactcc gggcacttaa gaccttcctg gagtacgctg agcaccgtaa
     2581 cctggagagc ctggattccc ataccactga agcaagctcc ccattggaat cctccatcag
     2641 atcatacctg gaggaggagg gcctagaggt gcaccagcag gtgggctgcg ctggctaccg
     2701 cctggacatg gcggtggtgg accctgagga tccggaccgt tacctccttg gcattgaatg
     2761 tgacggggcc atgtaccaca gtgccccggt ggcccgggac agggaccgcc tccgtcagca
     2821 gatactggag ggcctgggct ggaacatcta caggatctgg tccacagact ggtaccgtaa
     2881 ccgagaagaa agcctaaacc gcctgtacat ggctatccag gaagcccggg agggtaagaa
     2941 gaagccagag gtggagttga tcgaggagga aaccctggtg gaggaggagg cacccccggc
     3001 gccggttgtg gttagcctgg aggatatgat acccgcctac cagttatgca aggatctgga
     3061 gcttagcaag gataaacccc tccacgagaa actgccccag gagctggccc cggccatgcg
     3121 ccgggtggtg gataccgagg gccccctaca tctaagtgaa ctctacaaga ggatccgcag
     3181 ccactggggg ctgaaacgat ccggacggcg gatcaaggag accctggacg catcactcca
     3241 gttagccctg gataagggac ttttcaccat ccaggacgac ttcctatttt ccagaacagg
     3301 ggagatccgg atccggcgcc ggggcgagga catccccgcc cgcattgaac tgatcagtga
     3361 tgaggaaatt aagaaggcag tgctactggt catcgagacc cagcacgcca cctaccccga
     3421 ggaactggtc acccaagtgg cacgcctatt tggcttcaaa tccacacggc aggccacccg
     3481 ggaccgggtt aaccacatac tacggggtta cctggagcag ggcgaactgg tggccatgcc
     3541 caacggcatg atcaacttcc ccagaaaaga ctagttgtca cattttttta gaggaaaagc
     3601 tgatggtaga aaatgttcta gaaagactaa ctaatgatcc ccgatttcag gataaggtgg
     3661 aacacctaga ggaactggag gcccaggagg cccagtacac ccctgtagat gacttacccc
     3721 ccaacatcga ggactacctt cgaagggaga agatcaaact ctaccagcac caggcccagg
     3781 ccatacaatc tgtgagggag ggtgaaaaca tcctcatcac cacccccaca gcctcgggca
     3841 agaccctggc cttcaaccta cctgtactgg agaagctctc cctggatccc caggccaccg
     3901 cactctacat ctacccggca aaggccctgt ccaatgacca gctgcgggta ctgcgtggcc
     3961 tggaaaaggc ttgtgacctg gaactggatc cggctatcta cgatggggac acaccacgtg
     4021 acatacgtcc ggatatccgc cgacactccc gggtgggcat caccaaccca taccagttac
     4081 acctcatatt ggcttggcat caccagtggg agcggttcta ctcccagttg aagtttgtag
     4141 taatcgacga ggcccaccag tataggggtg tcttcggatc caacgtggcc ttcctcatca
     4201 ggaggttaag gcgtatctgc aactactacg gcagcaaccc ccagttcata ctgtcctcgg
     4261 ccaccctggc caacccagag gagttcagcc agaaactggt gggccagtcc ttccaactca
     4321 tagaccagga cagctccccc cgtggtagga agcacttctt gttctacaac ccccatctaa
     4381 ccccaggtgc atccacccac caggaaacaa cccacctatt tttattcttc atcagacagg
     4441 gcctgcagac tttgtgcttc accctcagcc gccgcatggc ggagctcatt gcccgttgga
     4501 gtttgcagga actccaggac actgaccctg aactggcagg ccaggtcaca gcctaccggg
     4561 ctggctacct ggccccggag aggaggaaga tcgagtatgg attgaaaaca ggggcgctat
     4621 tgggggtgac caccaccaac gccctggaac tgggcatcga tatcggcagc ctggatgcgg
     4681 tgatcatctc tggctacccc ggcaccatga tctccacctg gcaacaggcc ggccgctccg
     4741 ggcggaaaaa caacgactca ctggtggtgc tggtggcatt tgaggggccc ctggaccagt
     4801 acttcatgaa aaatccccag gtattctttg accggcacca tgagaatgcc atcatcgacc
     4861 tggagaacgt gcacatcacc ttcaaccacc tcctatgtgc catgagcgaa ctacccctaa
     4921 gccgggagga aataacgagc tgcttccaga ccagtccagg ctttatggga cgcctgatcc
     4981 gcagccacca ggtccgggag agccaggaca ggttcacata ccaggggaag ggcagccctg
     5041 ccttccaggt gggactgcac cacatatccc gggacgagtt ccaggtttac catgatggat
     5101 atctgctgga aacactggac cggccacatg cctacctgga agcccaccac ggagcggtgc
     5161 tcatcaacaa gggagagacc tacctggttg aggagttcaa cctccagaag caggaggtgc
     5221 gggtgcgtaa aaggaacctg gactaccata cccaggtcct gaaggaggtg gatgtccggg
     5281 ttctggagtg gattcatcac aaacaaatag gccagttcag ggtgcactac ggggaggtgg
     5341 aggtcactga acactaccac cgctacaagc gtatcagcca gggcaaaact gtggccaccc
     5401 accccctgga tctgccccca ctccacttcc gaaccaaggg attatggttc accctccccg
     5461 gagatgttga ggaccagatc aaccattaca tcagtggaca ggaggtctac gaggggggtt
     5521 tgcacggtac tgaacacgcc cttattgcca tgttcccctt aaaggtactg tgtgaccgct
     5581 ttgatattgg aggcttgtcc accccccacc accccgatac caggggggct accattttca
     5641 tctacgatgc cttccagggg ggtattggat tggctgagaa ggcagtggag ctcatggagg
     5701 aactggtgga gcttacctat gatatggtgc gcggctgcca gtgccgggat ggctgtccct
     5761 cctgcatcta ctcccccaag tgtggtaacg acaaccagcc cctgcacaag agaagcaccc
     5821 agtacatact cagccggatc ctaaagctca tgggcaaggt agatgaggcc gatatccacc
     5881 tagaaggggc aactgcacac aacggatcag agtaaatcca gatgtatagt tacgggtggc
     5941 ccagaaggag tgattaggtt cttgacaggc ttaaagttga tgcaggtggt gattcatcct
     6001 ttcctaccca gaggatcccc acatctctcc tcctaggtat cccaatgtat ggccatttac
     6061 aagtcccccc tattcattct tacggttttt ccagacttga aatcacacca gaaccggatt
     6121 gttggataca gggtaagggg ggggccactg gaatgtcaac cacttataaa gacatcccac
     6181 atccactagg ggatggcgtc taagaatccg cttgtcaggc tagatctatt ccatcaaagc
     6241 ccccaacttc ttttttttat atggatgtgg atcattactg gaggctgcgc tttctttggc
     6301 agggacatcc aaatggcgtt gtttttcata ccagcaatat tgttatgtct aatcaaggaa
     6361 attattaata ctagttgggg gtaattaaaa aaatacaggg gggtaatcaa tgtgaaggaa
     6421 actaaccaat atggtgattg ttatggaaga agtaaacaga ctgtaccagc aaggttacat
     6481 agagaagatc aacccctccc cactcaagag tcaactgtcc ctaaaagaag cccgtatatg
     6541 gctgaaggag gctagactaa attgtaacag tggaggttac cgttcctcgc gtattgcctg
     6601 ttacatggca ttcttccatg ccgcccggtc tgtgttacag cgtgatggtt accaggaaga
     6661 caaaccagaa tacctggccc tttatctaga aaaatataac agggaaggac ttctggagga
     6721 agaatggacc caactactgc aatggataat gaacctgcac caccaggacc tgtaccactt
     6781 ccaggccttc ccctcccctg aagaggctga tgaggctatc gagcagggaa ctgacttcgt
     6841 ggaaaggatc agcatcctcc tggacgagac caactacgcc aaaaaggcct tcctgaaaca
     6901 gaacgccgtg caaaggg
//
LOCUS       DAOQVW010000011         6907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_12092_length_6907_cov_3.286194, whole genome shotgun sequence.
ACCESSION   DAOQVW010000011 DAOQVW010000000
VERSION     DAOQVW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12092_length_6907_cov_3.286194"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="mtrE"
                     /locus_tag="PKK85_00455"
     CDS             complement(<1..832)
                     /gene="mtrE"
                     /locus_tag="PKK85_00455"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit E"
                     /protein_id="HNS24527.1"
                     /translation="MEAMIAGLGVVALMGAAATIAGAAEDLESDVGSQSNPNSQVQLA
                     PQMGNLHRMFNKAVSGEPVSMGTWAGIAGGVAFVLMNSLSLPVIMSIASGAAIAALVH
                     ATFATSSHLGRIVSQSQFNQPLFLDVVVQHLGPIAGHGFITTFCIVGLSYLMTLQIPG
                     FAHPFALPFLAVLWGITIGAIGSSTGDVHYGAEREYQQYPFGGGIPVAIHGDITRQSE
                     LGPRNSMDVVYFCAKFGGPVTGFAFGLIVFLSFWVTIVFGSAGGIVASFVIILLLIYI
                     N"
     gene            complement(889..2541)
                     /gene="mcrA"
                     /locus_tag="PKK85_00460"
     CDS             complement(889..2541)
                     /gene="mcrA"
                     /locus_tag="PKK85_00460"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     alpha"
                     /protein_id="HNS24528.1"
                     /translation="MADKMFIRALKEKFEEAPEETKTSFYKYGGWQQSERKREFVAAG
                     KEVAKKRGIPQYDPDVGTPLGQRVLMPYQVSTTDTFVEGDDLHFVNNAAMQQFWDDIR
                     RTVIVGLDSAHNVIEKRLGKEVTPETITHYLETVNHAMPGAAVVQEHMVETHPTLVAD
                     SYVKIFTGNDEIADEIDPAYVIDINKMFPEHQAEQLKAEVGDSMWQAVRIPTIVSRTC
                     DGGTTSRWSAMQIGMSMISAYKQAAGEAATGDFAYAAKHAAVIHMGTYLPVRRARGEN
                     EPGGIAFGFLADICQSSRKYYEDPVRVSLDVVASGAMLYDQIWLGSYMSGGVGFTQYA
                     TAAYTDNILDDYCYFGKEYVEDKFGIASAPNNMDTVLDVASEVTFYGLEQYEEYPALL
                     EDQFGGSQRAAICAAASGCSTGYATGNAQTALSGWYLSMYLHKEQHSRLGFYGYDLQD
                     QCGASNVFSIRGDEGLPLELRGANYPNYAMNVGHQGEYAGISQAAHAARGDAFVLNPL
                     VKIAFADDNLTFDYSEVRAEFAKGALREFEPDGERALISPAK"
     gene            complement(2559..3308)
                     /gene="mcrG"
                     /locus_tag="PKK85_00465"
     CDS             complement(2559..3308)
                     /gene="mcrG"
                     /locus_tag="PKK85_00465"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HNS24529.1"
                     /translation="MAQYYPGTTKVAQNRRNFSDPDYELEKLREISDEDVVNILGHRA
                     PGEEYPSVHPPLEEMDEPDDAIREMVVPLDGAKAGDRVRYIQFTDSMYFAPAQPFLRS
                     RSYLCRFRGADAGTLSGRQIIETRERDLEKYSKYLLETEYFDPARSGIRGKTVHGHSL
                     RLDEDGMMFDMLRRLILNKDTGKVEAVKNQIGDELDEPVVLGEPLDEATLKEKTTIYR
                     KDGEAYRDDKDAVEILHRIHVVRSEAGYSPE"
     gene            complement(3310..3906)
                     /gene="mcrC"
                     /locus_tag="PKK85_00470"
     CDS             complement(3310..3906)
                     /gene="mcrC"
                     /locus_tag="PKK85_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase I operon protein C"
                     /protein_id="HNS24530.1"
                     /translation="MIGKCTHVVDCRETMGMGEGGGIAQRGTFAECGNEVLAIAMSPG
                     RRHITKPVCEITFALREANILTSTLVLNAGAGVPQDAPTAGAGSLMGLTVKEEEQIKR
                     FKVIVVHLGGVKHHITYKARLILRHVDRPCVIICEYPVDFEDFAKIGVKTKAVMPEEP
                     NTKGEIVDIVSGVIRGETCPQEKLDEIIKKVRLALGGA"
     gene            complement(3909..4340)
                     /gene="mcrD"
                     /locus_tag="PKK85_00475"
     CDS             complement(3909..4340)
                     /gene="mcrD"
                     /locus_tag="PKK85_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase operon protein D"
                     /protein_id="HNS24531.1"
                     /translation="MDIEIFPHRLLNPETTEQLLNELEVMDGVKRMVVHGPRLPPEEA
                     GSSDRTITIQGEKLQLQVQTGRILLEIDEEETIDEVRAICENYLPFGFNIYVGTFIRK
                     EKTVTDDIKYAGLDQIPEEMIGLTDQNAKLSERATVIKRKD"
     gene            complement(4364..5695)
                     /gene="mcrB"
                     /locus_tag="PKK85_00480"
     CDS             complement(4364..5695)
                     /gene="mcrB"
                     /locus_tag="PKK85_00480"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HNS24532.1"
                     /translation="MAKFEDKVDLYDDRGNLVEEQVPIEALSPLKNPAIKRIVQGVKR
                     TVAVNLEGTENALKTAKVGGPACKILGREMDLDIVGNADAIAAKAKEMIQVDEGDNTT
                     VDLLAGGKRALVQIPDSRFEAAAEYSAAPLVTASAFIQAIIDVCDVNMYDANMVKAAI
                     LGRYPQSVEYMGGNIATMLDLPQKLEGPGYALRNIMVNHVVAATLKNTMQTSALSSIL
                     EQSAMFEMGDAVGKFERMHLLGLAYQGMNADNLVFDLVKDNGAEGTVGSVINDLVERS
                     AADGVVGVEKELNGFKVYKTDDLAKWNAYAAAGLIAATMVNQGAARAAQGVSSTILYF
                     NDILEFATGLPSVDFGRAEGVAVGFSFFSHSIYGGGGPGIFNGNHIVTRHSKGFAIPC
                     VAAAMALDAGTQMFSPEATSGLIKEVYSQVDEFREPLKYVVEAAVDIKDQI"
     gene            5975..>6907
                     /gene="mmp10"
                     /locus_tag="PKK85_00485"
     CDS             5975..>6907
                     /gene="mmp10"
                     /locus_tag="PKK85_00485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515402.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HNS24533.1"
                     /translation="MQIIADIGGIPGKNCRGFCKYCYFRKVKTLEPFGCRQCSPGKLG
                     CEYCTEGIREIKSEFIPPFLVLGNLQNSLMMGNFQGNDLKINISGGGDLSCYPYLLDL
                     TSAISQWGIPIHLGYTSGKGIDDPQMAKQLLSHGVEEVTYTIFSTNPLLRKTWMKDPT
                     PEASVEAFKIFCENSEVHAAAVIVPGVNDGEELLKTCNHLEEWGANALILMRFANFRN
                     QGLILGNEPIVDGLTPHSLEEFENLVWKINQDYQFRVTGTPLCDPETDAPFALSLDKK
                     KEYVDILSEVKSEATLLTGKVAKPFLEKIIENIGA"
BASE COUNT         1666 a         1632 c         1579 g         2030 t
ORIGIN      
        1 tgttgatgta gatcagtagt aagattatca cgaaagatgc gactattcct ccagctgatc
       61 caaatacaat ggtgacccag aagcttagga atactattag tccaaatgcg aaaccggtta
      121 ctggtcctcc aaatttagca cagaagtata caacatccat cgagtttctg ggacctagct
      181 ccgattgccg ggtgatatca ccgtgaatag caacggggat acccccaccg aatggatact
      241 gctggtattc tctttcagca ccatagtgaa catctccagt tgatgaacca atagctccga
      301 tggttattcc ccataatact gcgaggaatg gcagtgcgaa tgggtgagcg aaaccgggta
      361 tttgtaaagt catcaggtat gataatccta ctatacagaa cgttgtgatg aatccgtgac
      421 cagcaatggg tcccaaatgc tggacaacaa catccaggaa cagtggttga ttaaattgag
      481 attgactcac tatccttccc aagtgggatg aagtagcgaa agtggcgtga accaaagctg
      541 ctatggctgc tcctgatgca atagacatta tgacaggtaa gctgagtgaa ttcattagaa
      601 caaaagcaac gccaccagct atacccgccc atgtacccat ggagacgggt tcgcctgaga
      661 cagccttgtt gaacattctg tgtaagttac ccatctgggg tgccagttgt acctgagagt
      721 tggggttact ctgtgaacca acatcggatt ccaggtcttc tgcagcacca gcgatggtag
      781 cagctgcacc cataagagca acaacaccta atcctgctat catagcctcc atataattcc
      841 tcctctaatt ttttaaataa ataaaaaaaa taggtgtttg cgtaacacct attttgctgg
      901 gctaattaaa gctctttcac cgtctggttc gaattctcgt agagcacctt tggcaaattc
      961 agccctcact tcggagtagt cgaaggtcag gttgtcgtcg gcaaaggcga ttttaaccag
     1021 tgggttaagt acaaaggcgt ctccacgggc ggcgtgtgca gcctgagaga taccggcgta
     1081 ttctccttgg tgacccacgt tcatggcgta gttggggtag ttggctcccc tcagttccag
     1141 tggcaatcct tcgtctccac ggatggagaa tacgttggaa gcaccacact ggtcctgcag
     1201 gtcgtatccg tagaatccga gcctggagtg ttgttctttg tgcaggtaca tagacaggta
     1261 ccatccactc agagcagtct gggcgtttcc agtggcgtaa ccagtggaac aaccggatgc
     1321 tgcagcacag atagcagctc tctgggatcc accgaactgg tcctctaaga gtgcggggta
     1381 ttcttcgtac tgttcgagac cgtagaatgt tacttcagat gctacgtcca gcacggtgtc
     1441 catgttgtta ggtgccgatg ctataccgaa cttgtcttcg acgtattctt taccgaagta
     1501 acagtagtcg tcaaggatgt tgtcggtgta agcagcggtt gcgtattggg tgaatccaac
     1561 accaccagac atgtaggaac ctagccagat ctggtcgtac agcatggcac cggaagccac
     1621 cacgtccagg gatactcgga ctgggtcttc gtagtactta cgggaggact gacaaatgtc
     1681 agctaagaat ccgaaggcaa tacctccagg ttcgttttcc ccacgagcac gtcgaacagg
     1741 taggtaagta cccatgtgga taactgctgc gtgtttagct gcgtatgcga agtcaccagt
     1801 tgcggcttcc ccggcagctt gtttgtaggc agaaatcatg gacataccga tctgcatagc
     1861 tgaccatcgg gaggtggtac ctccgtcaca ggtacgggat actatggttg gtatgcgtac
     1921 tgcctgccac atactgtccc caacttcagc tttgagttgt tcagcttggt gttctgggaa
     1981 cattttgttg atgtcaatga cgtaggcagg gtcgatttca tctgcgattt catcgttacc
     2041 agtgaagatt ttcacgtaac tgtcagccac cagtgttggg tgggtttcca ccatgtgttc
     2101 ttggacaact gcagcaccag gcatagcgtg gttcacagtt tccaggtagt gggtgatggt
     2161 ttctggggta acttctttac ctaacctttt ttctataacg ttgtgggctg aatctaaccc
     2221 cacaataacg gtccttcgga tatcgtccca gaattgctgc attgcagcgt tgttgacgaa
     2281 gtgaaggtcg tcaccttcca cgaaggtgtc ggtggtagaa acttggtaag gcatgaggac
     2341 cctttgaccc agtggagtac ccacgtcagg gtcgtattga ggaattccac gttttttagc
     2401 cacttcttta ccggcggcca cgaattccct tttcctctca gactgttgcc atcctccata
     2461 tttgtagaag gatgtcttcg tttcttcagg agcttcttcg aatttttctt tcaatgctct
     2521 tataaacatc ttatcggcca taatcataca cctcctgatt attcaggact gtaaccagcc
     2581 tcggatcgga ccacgtgtat tcggtgcagt atttccactg catccttgtc gtctcgatat
     2641 gcttcaccat cttttcggta gatggtggtt ttttctttga gggtggcttc atccagtggt
     2701 tctcccagta ctacgggttc gtccagctcg tctccgatct gatttttgac tgcttccact
     2761 ttaccggtgt ctttgtttaa gatcagcctt cgcagcatgt caaacatcat accgtcttcg
     2821 tctaaccgga gtgagtgacc gtgtacagtt ttacccctga taccacttct ggctgggtcg
     2881 aaatattcgg tttccagcaa gtatttggag tatttttcca ggtctctttc tctggtttca
     2941 ataatttgcc gtccggatag ggtaccagcg tcggctccac ggaacctgca caggtaggat
     3001 ctggatctta agaatggctg agctggggca aagtacatgg agtctgtgaa ctgaatgtat
     3061 cgtacccggt ctcctgcttt tgcaccatca agtggcacta ccatttcacg aatagcgtcg
     3121 tctggttcat ccatctcttc tagtggtggg tgtacacttg ggtattcttc accgggagcc
     3181 cggtgcccca ggatgttcac cacatcctcg tctgagattt ccctgagctt ttctagttcg
     3241 tagtcagggt cactaaaatt tcttcggttt tgagcaacct tagttgttcc aggataatat
     3301 tgtgccatat catgcacctc ctaatgctaa tctcaccttt tttataatct catccaattt
     3361 ttcttgggga caagtttctc ctcttataac tccgcttact atgtccacaa tttcaccttt
     3421 ggtattgggt tcttcgggca taacagcctt agttttgaca ccgatttttg caaaatcttc
     3481 aaagtccacc gggtattcac agatgattac acagggcctg tcaacgtgcc tgaggatcag
     3541 gcgggccttg taagtaatat ggtgtttaac ccctcccaga tgcactacga tcactttgaa
     3601 tcttttgatc tgttcttctt ccttcaccgt tagccccata aggcttccgg ctccagcagt
     3661 aggtgcatcc tgtggtactc cagccccggc gttaagtacc agcgtgctgg taagtatgtt
     3721 agcttcccgc agggcaaaag ttatttcaca aactggcttg gtaatatgtc tccttccagg
     3781 ggacatggct attgccagaa cctcatttcc acactcggca aaagtaccgc gctgcgctat
     3841 tccgcctcct tcacccattc ccatggtttc tctgcagtca acaacgtggg tgcatttgcc
     3901 aatcatgttc aatctttcct cttgattacg gtggctcttt cactgagttt agcgttctga
     3961 tcagttaagc cgatcatctc ttcgggtatc tgatccaggc cagcgtactt gatatcgtcg
     4021 gttactgttt tttccttccg gatgaaggtt cccacgtaaa tgttgaaacc aaagggcagg
     4081 taattttcgc atatcgctcg tacttcgtcg atagtttctt cttcatcgat ttcaagaagt
     4141 attctaccgg tttgtacctg tagttgtagt ttttcgcctt ggatggttat ggtacgatct
     4201 gagcttcctg cctcttccgg tggaagtctt ggtccgtgca ccaccatcct ctttactccg
     4261 tccatcactt ccagttcgtt tagcaactgt tctgtggttt cagggtttaa tagtcggtgt
     4321 ggaaatattt cgatgtccat ttaactcgta gcccccaatt ggattagatt tggtctttta
     4381 tgtctactgc ggcctccacc acatatttta gtggttctcg gaattcgtcc acttgactgt
     4441 acacttcttt gattagtccg gaggttgctt caggtgagaa catctgagtc ccggcgtcta
     4501 atgccatggc tgcggccaca catgggatgg cgaaaccttt actgtgtcgg gtcacgatgt
     4561 ggttaccatt gaatatacca ggacctccac caccatagat agagtggctg aagaaggaga
     4621 atcctacagc tacaccttcg gctcggccga agtctacgct tggtaatcca gttgcgaatt
     4681 caagaatgtc gttgaagtac agaatggttg aggatacacc ttgtgcagca cgggcagcac
     4741 cttggttgac catggtagca gctattaatc ccgcagcggc gtatgcattc catttagcta
     4801 agtcgtcggt tttgtagact ttgaaaccgt ttaattcttt ttcgacacca acaactccgt
     4861 cagcggcaga tctttccacc aggtcgttga taacggatcc tacagttcct tcggcaccgt
     4921 tgtctttaac taggtcaaac actaggttat ctgcgttcat accttggtaa gccagtccga
     4981 gaaggtgcat tctttcgaat ttacctactg cgtctcccat ttcaaacatt gcagattgtt
     5041 ccaggatgct ggatagagca gatgtttgca tggtgttctt taaggtagcg gctactacgt
     5101 ggtttaccat gatgttacgt agagcgtatc cagggccttc cagtttctgt ggtaagtcca
     5161 gcatggtggc aatgtttcca cccatatatt ccacagattg tggatatcgt cctaagatgg
     5221 ctgctttcac catgttggcg tcgtacatgt tgacgtcaca gacatcaatt atggcttgta
     5281 taaatgctga agcagttacc agtggtgcag ctgaatattc agcggctgct tcgaaccttg
     5341 agtctggtat ttgcaccaga gccctttttc cgccggctag tagatcgaca gtggtgttgt
     5401 caccttcatc tacttggatc atttccttgg ctttggctgc aatagcgtct gcattgccta
     5461 caatatccag atccatttct cgacccagta ttttacatgc tggtccgccg acttttgcag
     5521 tctttaaagc gttttctgta ccttccaggt ttacagctac agtcctttta acaccttgca
     5581 caattctttt gatggcaggg tttttcaacg gactgagggc ctctataggt acttgttctt
     5641 caacaaggtt gcctctgtcg tcgtacaagt cgaccttatc ttcaaacttt gccattattt
     5701 ccctcctaca gattcttgta tactaattct tcgtgccaaa tcgaaagaaa agtagaaaaa
     5761 aaactactaa tcccgggtta aaaccctctt tggtacaatt tagtatacga acctcatttt
     5821 ctcccatgta tattattaaa gtttcttttt gattcaagta gaaagattta tatgtagacc
     5881 agtggtagaa ttctgtccca ataccttaca catttccttg agtgatgaac aaaaaaatag
     5941 cccatatatt aattataaag acatagtaga taacatgcag ataattgccg atataggcgg
     6001 aatacccggt aaaaactgcc gtggtttctg taaatattgt tattttcgta aagtaaagac
     6061 attagagcct tttggttgca gacaatgttc acctggtaaa ttagggtgcg aatattgtac
     6121 cgaaggcatc agggagatca aatctgaatt tataccccca ttccttgtat tggggaacct
     6181 tcagaattct ctgatgatgg gaaatttcca gggaaatgac ttgaaaataa atatcagtgg
     6241 tggtggcgat ttaagttgct atccctatct tttagatctt acctctgcga tttcacaatg
     6301 gggaataccc atccacctgg gctacaccag tgggaaagga atagacgacc cccagatggc
     6361 aaagcagcta cttagccatg gagtggagga ggtgacttac accatttttt ccaccaaccc
     6421 cttgctccga aagacctgga tgaaggatcc cactcctgaa gcatctgtag aggcctttaa
     6481 aatattctgt gaaaactctg aagttcacgc tgcggctgta atcgtgcctg gggtgaacga
     6541 tggggaagaa ctgttgaaaa catgcaacca tctggaagag tggggagcaa atgccctgat
     6601 ccttatgaga tttgctaatt tcaggaacca aggacttata ctaggtaatg agcccattgt
     6661 agatggttta accccccata gtttggagga gtttgaaaat ctggtatgga aaataaacca
     6721 ggactaccaa ttccgagtaa ccggaacacc cctgtgtgac ccggaaactg atgccccatt
     6781 cgctttatct cttgataaga aaaaagagta cgtggatatt ctttcagaag taaaatcgga
     6841 agccacttta cttactggaa aggttgccaa gcctttcctc gaaaagatta tagaaaatat
     6901 tggggcc
//
LOCUS       DAOQVW010000012         6828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_12257_length_6828_cov_3.144987, whole genome shotgun sequence.
ACCESSION   DAOQVW010000012 DAOQVW010000000
VERSION     DAOQVW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12257_length_6828_cov_3.144987"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKK85_00490"
     CDS             <1..249
                     /locus_tag="PKK85_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24534.1"
                     /translation="IVSIVSSIVSMILAIFAIMFSRRVEERLKNNFLRLKETMELNRD
                     QAEACLYNIDKEAEAIKNTVADSHSQIHESLQMLKKEK"
     gene            301..966
                     /locus_tag="PKK85_00495"
     CDS             301..966
                     /locus_tag="PKK85_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HNS24535.1"
                     /translation="MEIREKYPGVYQLEDNVATVNLKPQVKVYGEKLVDYEDKELRIW
                     DPRRSKLAAAIRNGLKTFPFREDSRVLYLGASAGTTPSHLSDIIVDGIVFCVEFSPRM
                     MRELLQLSRERENLIPILDDATKPHHYQPLMEEVDVVYSDVAQPRQTELFMDNMRRFL
                     KEDGLGLMAIKARSIDVTQRPKKVFRQEESKLKAAGFRVVEKVDLEPYEKDHMVVVCE
                     FSF"
     gene            complement(1384..2526)
                     /gene="coaBC"
                     /locus_tag="PKK85_00500"
     CDS             complement(1384..2526)
                     /gene="coaBC"
                     /locus_tag="PKK85_00500"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNS24536.1"
                     /translation="MRIVLCATGSVAAVETVKLARELKRQGFKVKCFMSDGACDIINP
                     YALEFATGEKVITKLTGDIEHVKYAREDLILVAPATANVISKFAYKIADNPINTLLVT
                     AYGYDTPIIMVPSMHESMYRAVEENINKLKGEGILFVEPRQEENKAKFPSIQDIVLKV
                     ERNTSKRDLAGKKVLVSAGATYEDIDPIRGITNRSSGKMGVEIAKEAFRRGADVTLIC
                     GRVEIGLPSIFRVVKVKSALEMQLEVEGLVLDQDVFLSAAAVSDFTLEKETHKVSSHE
                     DITLQLRPAPKIINRVKAINPEIFLVAFKADYNVSNEEIVESARQRMDESRADLVVAN
                     DVAVPGAGFGSDENQVLLIDEHVRKVPLTSKEEIAGKIIDKVVQRL"
     gene            complement(2568..3071)
                     /locus_tag="PKK85_00505"
     CDS             complement(2568..3071)
                     /locus_tag="PKK85_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNS24537.1"
                     /translation="MVLPVCDVCLKSGILCQGCETKLKSGEISQMDLDIAKILFKLGD
                     GKISFKKTIEIGDVVIIITQKDQVGKIIGRGGKIVRELSKKVGRKIRVVGENSDLKNL
                     AQDILAPARISGINIVYGMDQKKKYKIRVRREDSRRIPAKIEVLNDIIKELTGEETLV
                     VIDRNEY"
     gene            complement(3510..4040)
                     /locus_tag="PKK85_00510"
     CDS             complement(3510..4040)
                     /locus_tag="PKK85_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNS24538.1"
                     /translation="MRRYLFLIFSLLIVVMVSGCITPDNNETRTFSQNNVTFQYPADW
                     AVVQTTSPDAVAAVADPSSVPSGTNSPTTQVVIQKPNVTKGTKLNKAYDDNYATFFNK
                     TDNQKVSEANITTSKLKALENIYISSSSGIEKQYRVVWLEKNGTIYVILCSSRKTDFE
                     NLQSSFDIIVNSFQVQ"
     gene            complement(4089..4640)
                     /locus_tag="PKK85_00515"
     CDS             complement(4089..4640)
                     /locus_tag="PKK85_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24539.1"
                     /translation="MIDIVPDKQKIIRNLQELEFDYSQGNISRETYTSQKNELNRLLE
                     TFETADRIKRLQGKGQVEKPLEYWTEKEEKKKKQEERELLLQRYISNPQPQSKASSIT
                     NSRWKTMLAIFLVVAFLVGTGFGALIMQSPSEPSGDSLVANESAFPVKNTTNLTKTNT
                     TTTKTTTTKKNTTTPTTPTNTTG"
     gene            complement(4633..5442)
                     /gene="pheA"
                     /locus_tag="PKK85_00520"
     CDS             complement(4633..5442)
                     /gene="pheA"
                     /locus_tag="PKK85_00520"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNS24540.1"
                     /translation="MIIGFLGPAGTFTEEAATKISEDLRSFDSIVEVLEAVKVRDVDA
                     GVVPIENSVEGSVGVTLDILAQEDCLKIIKEIILPIRQHLLVNPGAKLEDVKEVYSHS
                     QALAQCRVYLEKLGIPTRAMLSTTAAAKAIKGKPERGAIGTSRAAEIYGLVIADYDVQ
                     DYPNNTTRFLCIGVNDHAYTGKDKTSIVFSLPDDHPGGLYELLGDFANFKVNLTKIES
                     RPSKEGLGSYIFFLDFEGHREEETIRNVLNSIKSKVGYVKILGSYPREGDD"
     gene            complement(5511..6713)
                     /locus_tag="PKK85_00525"
     CDS             complement(5511..6713)
                     /locus_tag="PKK85_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase"
                     /protein_id="HNS24541.1"
                     /translation="MQPERGRKFPEQFFFEYKSNTQISLKDFIELELPEILQLSPKQL
                     KGSFDRGNVKFYSEYGLNAIQLRKSIGHIEAGTMVSLGKNVEVIRGFPKIRRTLLLEP
                     VLKKYFKGEIALEEKMNGYNVRIARINNQTLAFTRGGFICPYTTKKANELMDMEEFFN
                     HHPHSVLCGEMVGTDNPYVSHYYPEIGKLGFRIFDVREKNTNQPWPILEKRKLLEEYG
                     LPTVKFFGIYDKSEAAGEILDIVKKMGEEDREGVVMKDPNMKIQPLKYTSSQAHVSEL
                     EYAFSYPFDLGQAFFFSRVIREGFQSFEMDKSDAEIKLRAHRIGESILQPMLETIRII
                     SDDKVAAEDLLIEVDSPDEAEDFARYLRSLGVIATVVDVRNGKAVIRRLHQSTSDKIR
                     NFLNGGLY"
BASE COUNT         1768 a         1586 c         1408 g         2066 t
ORIGIN      
        1 attgtatcca tcgtatccag catagtctcc atgatcctgg ccattttcgc tataatgttc
       61 tcccgcagag tagaagaacg ccttaaaaat aattttctga gacttaaaga gaccatggaa
      121 ctaaaccgtg accaggccga agcctgtctt tacaacatag acaaggaagc cgaggccatc
      181 aagaacaccg tggctgacag ccactcccag attcacgagt ccctgcagat gctgaaaaag
      241 gagaagtaga acatttagga ttacccaccg gaaagattat ccaagaattg aggatccatc
      301 atggaaataa gagaaaaata tcccggagtc taccagttag aggacaacgt agctactgtt
      361 aacctcaagc cccaggtgaa agtttacggc gaaaaattgg tggactatga ggataaagaa
      421 ctccgcatct gggacccccg tcgttccaag ctggctgcgg ccatcagaaa tggcctgaaa
      481 acctttccct tcagggaaga ctcccgggtg ctgtatctgg gggcgtccgc cgggaccaca
      541 ccctcccact tatcagacat aatagtagat ggtattgtat tctgtgtgga gttctcgccg
      601 cgtatgatgc gggaactctt acagttaagc cgagagcggg agaacctgat ccctatactg
      661 gacgatgcca ccaaacccca ccattaccag cccctgatgg aggaggtgga tgtggtctac
      721 agtgacgtgg cccagccccg gcagaccgag ctcttcatgg acaacatgcg ccgattcttg
      781 aaggaggatg gtctgggcct gatggccatt aaggcccgga gtatagatgt gacccagcgc
      841 cccaagaagg tatttcgcca ggaagaatcc aaacttaaag cagcagggtt ccgggtggtg
      901 gaaaaggtgg acctggagcc ctacgagaag gaccacatgg tggtggtttg tgagttcagt
      961 ttctgaggta tgaagagcgt ttctactcga tcataccatt tctatgtatc ctgcacgaat
     1021 agctggtaag tttctttgct atttctgaca ctgtatcctc cattttagat agaaatattc
     1081 tccataaacg gtgtcatttt cccacgcact attaaaagcc gcctgtaata ctgccacctg
     1141 ggttgacagt ccaatcaaca gccgaactta ccccgtgaaa agatataaca ccaacaaaat
     1201 ccccatgaaa accaaaatct attcataatt cacccaacat tagcatcaaa cattcatatt
     1261 gatattgctc actgatgaaa ttgttattat caatatcatc atcaaaagag aatttaaata
     1321 aacaactaag ccccatatct tcaaaaaata ggaaaaaact gtaaaataat tactataaaa
     1381 aaattaaagc ctctgcacca ctttatcaat tatcttaccc gcaatctcct ccttagaggt
     1441 taaaggaacc ttgcgcacgt gctcatcaat taagagaacc tggttctcat cggacccgaa
     1501 gccagcccca ggtaccgcca cgtcgttagc caccaccagg tcggcccggg attcatccat
     1561 cctctgccgg gcggattcca cgatctcctc attggagaca ttgtagtcgg ctttgaaggc
     1621 taccaggaat atctccgggt tgatggcctt cacccggttg atgatctttg gagccggtcg
     1681 taattggagg gttatatctt catgggagga tactttgtgg gtttcttttt ccagggtgaa
     1741 gtccgacacc gccgctgcgg aaagaaacac atcctggtcc aggaccagtc cctctacttc
     1801 gagttgcatc tccagggcgg atttcacctt caccactcgg aatatagatg ggaggccgat
     1861 ttcgacccgg ccgcatatta aagtaacatc cgctccccgc cggaaggctt ccttggctat
     1921 ttccacgccc atcttcccgg agctgcggtt ggttatcccc cggatgggat ctatatcctc
     1981 ataggtggct ccggcactga ccaggacttt tttacccgcc aggtctcttt tggaggtgtt
     2041 cctttctact ttaaggacta tgtcctggat ggatgggaac ttagccttgt tctcctcctg
     2101 tcggggttcc acgaagagta tgccttcacc tttaagctta tttatattct cttccaccgc
     2161 ccggtacatg gattcgtgca tggagggcac catgatgatg ggagtatcat aaccgtaggc
     2221 ggtgaccaat agggtgttaa tgggattgtc tgctattttg taggcaaatt tgcttatgac
     2281 gttggctgtg gcaggtgcta ccaggatcag gtcttcgcgg gcgtatttga cgtgttcgat
     2341 gtcaccggtt agcttggtga tgaccttctc tcctgtagca aattccagtg cgtaaggatt
     2401 gattatatca caggccccat cactcatgaa gcatttaacc ttaaatccct gtctttttag
     2461 ttcccgggct agtttaaccg tctccactgc tgctacactg ccagtggcac acagtacaat
     2521 gcgcataata acctccacct gtgactaact tagatgtaaa tagccatcta atattcattc
     2581 cggtcaatga ccaccagggt ttcttcccct gtcaattctt ttataatatc attcaagact
     2641 tcaatcttgg cgggaatacg acgagaatcc tctctcctaa cccgtatctt gtatttcttt
     2701 ttctggtcca tgccatagac tatgtttatc cctgaaatcc gtgctggtgc cagaatatcc
     2761 tgggctagat ttttcaggtc agaattctct ccaaccaccc ggattttcct gcccaccttc
     2821 ttggataact cccggactat ctttccaccc ctgcctatga ttttaccaac ctgatccttc
     2881 tgggtgatga ttataaccac gtcacctatt tcaatggtct ttttaaaact aattttccca
     2941 tctcccagtt tgaaaaggat tttggcaatg tccaagtcca tttggcttat ttctccactt
     3001 ttcagtttgg tttcacagcc ctgacagagt attccacttt tcaggcagac atcgcacact
     3061 ggcaacacca ttctcaattc ctcctcaact taagtgacta cggttcaaag gtatccctgg
     3121 gatgttatac cggtatctgt aatgatctta tcgttactgt ccaggttttc attgaatgat
     3181 ccggatttat gactttatcc taataaagtc acagtataac cttttttttg tagatgaata
     3241 aaataaaaac gtatttttag gcgagtgata aacactaccc tgataatatg gcagtctact
     3301 cttcaattat actatcccca ccatctacta tataattttt tgcttggttc atgaaatcat
     3361 gaaacagaaa taaattcaca ggctaaaaag gaaaacaggg ttcttttaaa aaaagataac
     3421 tcccattaag ataaattgaa gtgaaaaaat gcgttctaga ggttcgatca catcctcttt
     3481 gttctgtttc cataccggat tggtacgggc tactggacct ggaagctgtt gactatgatg
     3541 tcaaaactgg attgcagatt ttcaaaatct gtttttctgg aactgcacag tatcacgtag
     3601 attgtgccgt ttttttccag ccacaccacc ctgtactgtt tttctatacc tgaagaggaa
     3661 gagatgtaga tgttttccag ggcctttaat ttgctggtgg ttatgttggc ttcggagacc
     3721 ttctggttat cggtcttgtt gaaaaaggta gcgtagttat catcataggc tttgttgagt
     3781 ttggtcccct tggttacgtt aggtttttgg atgaccacct gggtggtggg agaattggta
     3841 ccggagggta cactactagg gtcagccact gcagccactg catcgggtga agtggtttga
     3901 accaccgccc aatctgcagg gtactggaag gtgacgttgt tttgactgaa ggtcctggtt
     3961 tcattgttat caggagttat gcaccctgag accataacca cgatcagtag tgaaaatatt
     4021 aaaaagagat atctgcgcat tttaacacct ctcgtcgttt atcttcatag actttattga
     4081 ctcataactt atccagttgt attggtggga gtggtgggag tggttgtatt ctttttggtg
     4141 gttgttgtct tggtggtcgt ggtgttagtc ttagtgagat tagtggtgtt tttcacagga
     4201 aatgcacttt cattagccac aagagaatca ccggaaggtt cagaagggga ttgcatgatg
     4261 agagcaccga atcctgttcc caccaggaat gccaccacca ggaatatggc tagcatggtt
     4321 ttccatctgc tgttggtgat ggagcttgcc ttactctgag gttgggggtt gctgatatat
     4381 ctctgcaata gtaattcccg ttcttcctgt tttttcttct tttcttcctt ctcagtccag
     4441 tactcaagtg gtttttcaac ctgtccttta ccctgaagcc tcttaatcct atcagcagtt
     4501 tcaaatgtct caagcaggcg atttaattca tttttttgtg aagtataagt ttcacgagat
     4561 atattgccct gtgaatagtc aaattcaagt tcttgtaagt ttctgatgat cttttgctta
     4621 tctggcacta tatcaatcat ctccttctct gggatatgag cctagaatct ttacgtatcc
     4681 taccttagat ttaatgctat ttaaaacatt ccgaatggtt tcctcttccc ggtgcccctc
     4741 aaagtcgagg aaaaaaatat agctcccaag cccctctttg gatgggcggg attcaatttt
     4801 tgtgaggtta actttaaaat ttgcaaaatc ccctaataac tcataaagac ccccaggatg
     4861 atcatcagga agagagaaaa ctatagatgt cttgtctttc cccgtataag catgatcatt
     4921 aaccccaata cacaaaaaac gggtggtatt attgggatag tcctgtacat cataatcggc
     4981 tattaccagc ccataaattt ctgccgcccg tgaagtacca atggctcccc tttcgggttt
     5041 tccttttatg gctttagctg ctgcagtggt actaagcata gcacgggtgg gtattcccaa
     5101 tttttctagg taaacgcggc attgggctag ggcttgggaa tgagaataga cttctttcac
     5161 atcttccaac ttggccccgg gatttactag cagatgctgc cggataggta atataatctc
     5221 ctttattatc ttcaaacaat cttcctgggc taaaatatcc agggtaaccc ctactgaccc
     5281 ttccacggaa ttttcaatag gaaccacccc tgcatccaca tcccggactt taactgcttc
     5341 cagtacttcc actatggaat caaaggatct taaatcttct gatattttgg tggctgcttc
     5401 ttcggtgaag gtgccagccg gacctaaaaa tccaattatc atgttttcac tccacattaa
     5461 ctagaaattg aaaataattc tccaagagaa ctttttttca tgaaaactag ttaatataat
     5521 ccgccattta aaaaattgcg tatcttatct gaagtagatt gatgtagcct cctgataact
     5581 gcctttccat ttcgaacatc cactacagtg gcaattaccc ctaaactcct caaatatcgt
     5641 gcgaaatctt cagcttcatc tggactgtcc acttctatta gcagatcctc ggcggctact
     5701 ttatcatcag atattattcg tatggtctcc agcatgggtt gaaggatgga ttctcctatc
     5761 ctatgcgccc ttaattttat ttctgcatcg gatttatcca tctcaaatga ttggaatcct
     5821 tcccggatca ctctactaaa gaagaatgcc tgtcccagat caaagggata gctgaaggcg
     5881 tactccagtt ctgaaacatg ggcctgcgaa gaggtgtact ttaagggttg tattttcata
     5941 ttcggatcct tcatcaccac tccttcacga tcttcttctc ccattttttt tacgatatcc
     6001 agaatttcac cggcggcttc tgatttatcg tagattccga agaatttaac ggtggggagt
     6061 ccatattcct caagcaactt cctcttttcc aggatgggcc agggttggtt ggtgtttttt
     6121 tcacgaacat cgaatattct gaatcctaat ttgccaattt cggggtagta atgggatacg
     6181 taggggttat cagtccctac catttcgcca cagagaactg agtgggggtg atggttaaaa
     6241 aattcttcca tatccattaa ctcattggcc tttttggtgg tgtagggaca tatgaagcca
     6301 cccctggtga aagccagtgt ttgattatta attctggcta ttcgcacgtt atacccattc
     6361 atcttttctt ccagagcaat ttcaccctta aaatactttt ttagtactgg ttcgagtagt
     6421 agagtccttc gtatcttggg aaatcccctt attacctcga catttttccc taaagaaacc
     6481 atggttccgg cttctatatg acctatactc ttacgtaatt ggatggcatt caatccgtat
     6541 tcactgtaaa acttaacatt acccctatca aaagatcctt taagctgttt aggacttaat
     6601 tgaagtattt ctggaagttc tagttcaata aagtccttta aacttatctg tgtatttgat
     6661 ttgtattcaa aaaaaaattg ttcggggaat ttcctccccc tttcaggctg catagttaaa
     6721 accacaagga tggaataagg gattagtcaa tccttttcca gctcagccat gagttgtagg
     6781 atgttggttt tggttatgat accctccatc ccttcaccca taaccaaa
//
LOCUS       DAOQVW010000013         6809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_12303_length_6809_cov_2.632070, whole genome shotgun sequence.
ACCESSION   DAOQVW010000013 DAOQVW010000000
VERSION     DAOQVW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12303_length_6809_cov_2.632070"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKK85_00530"
     CDS             complement(<1..869)
                     /locus_tag="PKK85_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNS24542.1"
                     /translation="MSSDNLILEKWFTRNGLAEGTCKMYRASISEFKEFVDKSLSELI
                     DEGMKQQMESIMPMNRSVNEYIFSFKNYLDKKGNAPGTVNHKIAAIKSFYKAYDIQFI
                     DIKTHKGDTVLDKNRGRFIAKEDIRKMLDVCNARNKAIIYTMALTGLAQNEVRNLTIR
                     QFIKYAGLSIGKELETIEDLFAYEDEILEDTIIFEIVRQKVNYRHHFLLPPEATKRII
                     TYLKERYHHRNKNRHIRSLNGPIFITNKGTKMTRTGIGNEIKRIGKLVGFKSDEEGAY
                     SYWRPHSFRKYFI"
     gene            1263..2771
                     /locus_tag="PKK85_00535"
     CDS             1263..2771
                     /locus_tag="PKK85_00535"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNS24543.1"
                     /translation="MEKEKYTTNKGHPLANDQASITTGAGSSYTLLQDVHLIEKLAHF
                     DRERIPERVVHAKGAGAYGYFEVTNDLSKYTRAKFLSEVGKKTEVFVRFSTVGGERGS
                     ADTARDPRGFAVKFYTEEGNYDLVGNNTPIFFVRDAIKFPDFIHSQKRNPQTNLPDAD
                     MFWDFLSLTPESVHQVTILFSDRGTPYSFRHMDGFSSHTFMWYNENNDYVWVKVHIKT
                     VLGNKTFTNDEAVKMAGENPNHATEDLYNAIEAGDYPEWKVYVQIMTPEEAKKYQFDP
                     FDITKVWYHGDYPLIPLGKMVLNRAPVNFFAEVEQSAFAPSNFVPGIGPSPDRLLQGR
                     LFSYEDTQRHRLGANFHQIPVNRPKNAKVNSYQRDGPMNVDEKGGSGPNYYPNTMNGP
                     EPDPSFAPPALEVQAIIDRHTRPTEDVDFIQAGELYRRVLDEQNKTNLVSNIVGHLKD
                     AKENIQYRQVALFYKADEEYGTRVANGLGLDVSKVKYLSAMNQEERVEATRT"
     gene            3099..3827
                     /locus_tag="PKK85_00540"
     CDS             3099..3827
                     /locus_tag="PKK85_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNS24544.1"
                     /translation="MWDTSNDYRLKVAEKSVELFINTMDRANLKGRWSKKLALEAARK
                     MIPEIQALYYSYLSPVEIAQSTYLEALEARVQEVKEALGGESWNRQFLQMADPGEREK
                     VENSLAKINFFLNTIYGLKQRILLGEINDPVMGVDIKKGEIVSVAKHPVAIKLLICNL
                     NLGERAITVVTNDLSLKNGDEVAVALLPPENFSGITSEGMFLGAEKGVLTGVEGDVGG
                     IPRGIPLEALNEVRNLVGAFLGRN"
     gene            complement(4132..5538)
                     /locus_tag="PKK85_00545"
     CDS             complement(4132..5538)
                     /locus_tag="PKK85_00545"
                     /EC_number="1.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008911 - lactaldehyde dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde dehydrogenase"
                     /protein_id="HNS24545.1"
                     /translation="MNMLINGKLIDKDDKVAVINPFNNETVDEVPLAGAEDVKKAILA
                     ASQARRLMERMSSRKLSRILIDVHQELKENLEDFAKLITLETGKTIRDSRMEMQRSLE
                     TIQLSAEECKRVYGETIPMDAAITGRSVFGFTIKIPLGVVAAITPFNYPVNLAIHKLA
                     PALGAKNSVVFKPSLTAPLAAMKLAEIMGHHLPPGAVNCVTGKSSEIGDELVTSDLIN
                     KISFTGSVATGLSIAHKAGLKKLTLELGGNDPLIVLDDADLDAAVSATMAGSYLNAGQ
                     VCIAVKRIIIQDGVADDFVDSLVEQTKKLKVGNPLDPKTDMGPLIDEYAALAVQSKVE
                     DAVDGGAQLLCGGQRKGAFYQPTVLDQVQPDMQVVWEETFGPVSPVIRVRTEKEAYQM
                     ANYTKYGLQAGIFTQDINRAKRAVKCIEAGSVLVNKQSTFRTDNMPFGGFKLSGIGKE
                     GVKYALDDMTRTKMVVIG"
     gene            complement(5643..5837)
                     /locus_tag="PKK85_00550"
     CDS             complement(5643..5837)
                     /locus_tag="PKK85_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HNS24546.1"
                     /translation="MMNDESGQTGAEMILIIGGIIVIAIVAAIVYRNYLQGLGDEINK
                     TEVQNVTNNIQDLGNTISNT"
     gene            complement(5859..6794)
                     /locus_tag="PKK85_00555"
     CDS             complement(5859..6794)
                     /locus_tag="PKK85_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNS24547.1"
                     /translation="MALIPPPLAPVSETMDRIFPDRYLVKLQETLIRAGMYVKASDLI
                     TLLFLVGFGLAIVAFVLFSIFGINPILGILLGLFAPAILVGVWLFFLMERRVDAIENG
                     TPDFLRQIASLLRAGVGIETALEDISKHGKGPLYDELKRTVIEIKIGSTFDESLLSMG
                     ERLKSKNLDRTFRMILEGRRVGGSLADVIETVAEDLRAVLSLQRERRANVMMSVMFLV
                     IAAIIAAPFALGMIMTYSSFIEVIGKPNVLQDAAMMSATGYIVIHSIIASLLIGIVMY
                     GSARKGVKYALLLVPAAYAIFYVITRLGPGLLGFT"
BASE COUNT         2006 a         1481 c         1324 g         1998 t
ORIGIN      
        1 ctaataaaat attttcgaaa cgaatgtggc ctccaataac tataagctcc ttcttcatcg
       61 cttttaaacc caactagttt gcctatcctc tttatttcgt ttccaatgcc tgttcttgtc
      121 attttggttc ctttgttggt tataaatatg gggccattta agcttctaat gtgcctattc
      181 ttgtttcgat ggtgatatct ttcctttaaa taagttatta ttcgtttagt ggcttctggg
      241 ggtaatagga agtggtgtct ataattaact ttctgccgga ctatttcaaa aataatcgta
      301 tcctctaata tttcatcttc ataggcaaaa agatcctcaa ttgtttcaag ttcctttcca
      361 atagataatc cagcgtactt aatgaattga cggattgtaa gattcctaac ttcattctgt
      421 gctagaccag tgagtgccat agtgtaaata atagctttat tcctggcatt acaaacatct
      481 aacatttttc taatatcttc tttagctata aacctccccc tgttcttatc aagaaccgta
      541 tcacctttat gagtttttat atcaataaac tgaatatcgt aagctttata aaacgattta
      601 attgcagcta ttttatgatt cacggtaccc ggggcgtttc ctttcttatc caaataattt
      661 ttgaaagaaa atatatattc gttcacagat ctgttcatag gcattataga ttccatctgt
      721 tgtttcattc cttcgtcaat taattctgaa agactcttat caacaaattc tttaaattca
      781 ctaatggatg ctcgatacat cttgcatgta ccttcagcta acccattacg agtgaaccat
      841 ttctctaaaa tcaaattatc gctcgacata ttattttctc cttttcgggg tataaaaaag
      901 atgtcgatat tgtgaaccca cagaaacgaa acttaaatat ttcgaaaaat aggcaccgtt
      961 aacttaattt aatgataaaa aaacttaaaa ttgataccga aaaattgtat cttcaattta
     1021 ttatttttaa atccaatgtc ttaatctgat tgaaattaag ataacgatag gataatttgt
     1081 tatttttgat ccgcttttct ttacttcccc tgtgcttaaa taaagatatc aacacggggc
     1141 tatatattaa ctaatcatag ggtggtagtt tttgggtgta ccctgatcca cctgttatga
     1201 ctagaaaaaa ctatatagaa ccaggttcta tttagaacta attagattgt ggaggttttc
     1261 tcgtggaaaa agaaaaatat acaaccaaca agggccatcc cttggccaat gatcaggcat
     1321 ccataaccac tggggcgggg tccagctaca ctttactcca ggatgtccat ttaatagaaa
     1381 aattagcaca ttttgatagg gaaagaatac ctgaaagggt tgttcatgcc aagggggccg
     1441 gggcttacgg gtactttgag gtgaccaatg acctgtcaaa atatacccgg gccaagtttc
     1501 tatctgaagt tggtaaaaaa acagaagtct ttgtaagatt ttcaaccgta ggtggagaac
     1561 gaggttcagc tgacacggcc cgtgaccccc gtggatttgc agttaaattc tacactgagg
     1621 agggaaatta tgacctggtt ggtaacaaca cacccatatt tttcgttaga gatgccataa
     1681 aattccctga tttcatacac tcacagaaga gaaacccaca aactaaccta ccagatgcgg
     1741 acatgttctg ggatttcctc tccttaactc ccgagtcagt tcaccaagta accatcctct
     1801 tttcagatcg gggcaccccc tattcattcc ggcatatgga tggattctcc agccacacct
     1861 tcatgtggta caatgaaaac aacgactacg tatgggttaa ggtgcacatc aagacagtct
     1921 tgggcaataa gacctttacc aatgatgaag cagtgaaaat ggccggagaa aatcctaacc
     1981 acgccactga agatctgtac aatgcaattg aggccggtga ctatccagag tggaaggtct
     2041 atgtccagat catgactccg gaagaagcca aaaaatacca gttcgatccc tttgacatca
     2101 ccaaggtctg gtaccatgga gactatccac tgattcctct gggaaagatg gtattaaacc
     2161 gtgccccagt gaacttcttt gcagaggtag aacaatctgc cttcgccccc tccaatttcg
     2221 taccagggat aggtccctcg cctgatcgtc ttttacaggg gcggctgttc tcctatgaag
     2281 atacacaaag acacagatta ggtgctaatt ttcaccagat accagttaac cggcctaaaa
     2341 atgccaaggt aaatagttac cagagggatg gtcctatgaa tgttgatgaa aagggaggat
     2401 caggacccaa ctactatccc aacaccatga atggacctga accagatcct tcattcgccc
     2461 ctcccgccct agaagtacag gccataatag atagacacac cagacccaca gaagatgtgg
     2521 actttatcca ggccggtgaa ctttatcgca gggtcttaga tgaacagaat aaaactaacc
     2581 tggtttcaaa catcgtggga cacctgaaag atgctaaaga aaatatccag taccgacaag
     2641 tagctttatt ctacaaagcc gatgaagaat atggaaccag agtagctaat ggtttaggtt
     2701 tagatgtttc taaagtaaag tacttatcag ccatgaacca ggaggaaagg gtggaagcca
     2761 ccaggactta gatccgatat caaaatgaat gggtaaaaat cggaatatga ccattaattg
     2821 aatttttttc caagtgcaga ttccaccgct attggcacgg gggcgtggca aactggcacg
     2881 gaccccaaca acattttttt ccaacctcct tcttccacat cttcgaggac aaaaaaaggt
     2941 ttattcaaaa cagccagtac tataaggtgc tatagtaaaa aattaagtgt taaaatcggg
     3001 tttttaaata ttcttttaaa tagacttcaa aattaatatt aataaccggc taaatctaac
     3061 ctgatcttga tctgaattgt tctagtaaag gtgacaccat gtgggatacc agtaacgatt
     3121 atcggcttaa agtggctgaa aaatctgttg aactctttat taacaccatg gaccgggcca
     3181 atctcaaggg aaggtggagt aaaaaactag ccctggaagc tgcccgtaag atgattccag
     3241 agatccaggc actgtattat tcttacctct ccccggttga aattgcccag agcacgtacc
     3301 tggaagcact ggaggcacgc gtccaggagg taaaggaagc attgggagga gaatcctgga
     3361 atcgtcaatt cctgcaaatg gctgaccctg gtgaaaggga aaaagtggag aactcattgg
     3421 ccaagataaa tttcttccta aacactatat acggactcaa gcagaggatt ctattgggcg
     3481 agataaacga cccggtgatg ggtgttgata taaaaaaagg ggaaattgtc agtgtggcaa
     3541 aacaccccgt cgctataaaa cttttaatat gtaatttaaa tctgggtgaa cgtgccataa
     3601 ctgtggttac caatgatcta tcccttaaaa atggggatga agtagcggtg gcactgttac
     3661 ctccagaaaa cttctccggc attaccagtg aaggaatgtt cctgggggct gaaaagggtg
     3721 ttttaactgg tgtagagggt gatgttggtg gcattccccg cggcattcca ttagaagctc
     3781 ttaatgaggt gcgtaacttg gtgggagcat ttttggggcg aaactaaaaa aaaatgtcaa
     3841 agagctgctg atgcaatttt ttttttgaag aaattgcgca gcaccttgaa atctattaat
     3901 tatctataac aaccatgtag acctttttta tggcggtaat ctattctacc tgtttgctgt
     3961 aagaattaac agttcctaat aatcgtccta caaatcccta aattatcatc aggggttgat
     4021 ggccacagta tcactgattt tgaattcttc catcaacttc ccatggacat ggttttttgg
     4081 taatttcttc tgatacatct ggatttggtc ttcaccatac atatcatcat cctatccaat
     4141 aaccaccatc ttggtgcggg tcatatcatc aagtgcatat ttaacacctt ctttacctat
     4201 accactcaat ttaaaacctc caaatggcat attatccgtc ctgaacgtcg actgtttgtt
     4261 cacaagaacc gatccggcct ctatacattt cacagctcgt ttggcccgat ttatgtcctg
     4321 agtaaaaatt ccggcctgca aaccatactt ggtataatta gccatttggt aagcttcttt
     4381 ttcagttcta acccttataa ctggggaaac tggtccgaag gtttcttccc agaccacctg
     4441 catatcgggc tgtacttgat ccaggacggt gggctggtag aatgccccct ttctttggcc
     4501 gccacaaaga agctgagcac ccccatcaac agcgtcttcc accttactct gcactgctaa
     4561 tgcagcatat tcgtcaatta agggacccat atcagttttg ggatccaagg gatttcctac
     4621 ctttaatttt tttgtttgtt ctactaaact atccacaaaa tcatccgcca ccccatcctg
     4681 tatgattatt ctcttaacag cgatgcacac ttgtccagcg ttaagatagg atccggccat
     4741 ggtggcagag acagcagcat ccagatcagc gtcatctaag actataaggg ggtcgttgcc
     4801 acctaattcc agggtaagct tttttaaacc agctttatgt gctatggata aaccggtggc
     4861 aacacttcca gtgaaggata ttttattgat caggtcactg gttaccaatt catctccaat
     4921 ttcactgctc ttgcctgtta cacagttaac tgctccgggg ggtagatgat gccccatgat
     4981 ttcagcaagt ttcatggcag ccaatggagc ggtgagggat ggtttaaaga ccacagagtt
     5041 tttagcaccc agggcaggtg ccaatttatg gatggccagg ttaaccggat aattaaatgg
     5101 agtaatagcc gctaccactc ctagtggaat ttttatggta aagccaaaaa cgcttctacc
     5161 ggtaatggca gcgtccatgg gtatggtttc cccgtacaca cgtttacact cctctgcaga
     5221 taactggatg gtttccaatg agcgttgcat ttccatccgt gaatctcgga tagtctttcc
     5281 ggtttccagg gttatcaact ttgcaaagtc ttccaagttc tctttcagtt cttgatgtac
     5341 atcaataagg atacgtgaaa gctttctgga agacattcgc tccattaatc tgcgggcctg
     5401 actagcagcc aaaatagcct tcttaacatc ttcagcacct gctaggggaa cttcatcaac
     5461 tgtttcattg ttaaatgggt taataactgc tactttatca tctttatcaa ttagtttccc
     5521 attgatcaac atattcatac ttaaccctcc gactagtgtc ttagcatagg ctagaagaaa
     5581 attaaataaa atagagaatg gattcagtgg acaaaaataa ccttgggcct tggataaaga
     5641 tgttaggtgt tgcttatggt gttgcctaga tcttgaatgt tgttggtcac gttctgcact
     5701 tcagtcttgt taatttcatc acccagaccc tgcagataat tccggtacac tatggcggca
     5761 acaatggcta taacaattat gcctccaatg atgagtatca tttctgctcc agtctgtcct
     5821 gattcatcat tcatcattcc gatattaaaa ccccctttct atgtaaatcc tagtaatccg
     5881 ggtcctaaac gtgttataac ataaaatatg gcataagcag ctggtactaa gaggagtgca
     5941 tatttcacgc ccttccgggc gctgccgtac atcacgatac caattaataa actggcaatg
     6001 atggagtgta ttactatata tcctgtggcc gacatcatgg cggcatcctg cagtacattt
     6061 ggtttcccaa tgacctcgat gaatgaagaa taagtcataa tcattcccag tgcaaatggt
     6121 gcggctataa tggcggctat aactaagaac ataacggaca tcatcacgtt tgctcttctc
     6181 tccctttgaa gagacaatac tgctctgagg tcttcagcca cagtttcgat cacatcggcc
     6241 aggcttcccc caacccttct accttccaat atcatacgga atgttctgtc aagatttttg
     6301 gacttcagac gctcccccat tgataacagg gattcatcaa aggtgcttcc aatcttaatc
     6361 tcgattacag tccgtttaag ttcatcgtag agaggtcctt taccgtgttt agagatatct
     6421 tccaaggcag tctcaatacc tacccctgct cgtaataatg atgcgatctg ccttaaaaag
     6481 tcaggagttc cattttctat ggcatctact cttctttcca ttaggaagaa caaccataca
     6541 cctacaagaa ttgccggagc aaataatcct aaaaggattc ctaaaatagg attaattcca
     6601 aaaatcgaaa acaacacaaa tgccactatt gctaatccga aaccaactaa aaaaagtaaa
     6661 gttataagat cagaagcttt aacgtacatt cctgcacgta taagtgtctc ctggagttta
     6721 accaggtacc tatctgggaa tattcggtcc atggtctcag atactggtgc taatgggggg
     6781 ggtatcaagg ccatattttt tcacctact
//
LOCUS       DAOQVW010000014         6803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_12319_length_6803_cov_4.149378, whole genome shotgun sequence.
ACCESSION   DAOQVW010000014 DAOQVW010000000
VERSION     DAOQVW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12319_length_6803_cov_4.149378"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKK85_00560"
     CDS             <1..240
                     /locus_tag="PKK85_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24548.1"
                     /translation="LPGNDTGDQATLTPAPHKNLYNLLEPGKKIGLKKLQDLSGRDGP
                     GLKKLIINLEKEGKLEPSGVGYWRVLEPESIDLKP"
     gene            complement(428..1348)
                     /locus_tag="PKK85_00565"
     CDS             complement(428..1348)
                     /locus_tag="PKK85_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24549.1"
                     /translation="MKKPTLEEIRRALILDNLITYREFNKNENKAYFKDKVSQKEICR
                     LVNWYYVDHETKDPADPKNWPVKPIQISKTLKGLEKDGLIIKEPYKTPGKGPAQNLIF
                     LKKDYDTLFKIIKEYNNPIFEPHYRGYLGDHLLNSPYGYELVNMDLVEAVLKEYKNLN
                     LEPIEKKRLLDLIKISPSALFTFIEAPYSDELLKPYLEGFKKKYLNNEQIKKALFLYI
                     LNGVGLDFTYRNPHSTPWKVEYEVNIKFSNWKDEDRLSNLKEEDIDLHFAPYLKDTLS
                     INKNTIKMTSTSIMGGISDTMILYKPFKDK"
     gene            1877..2074
                     /locus_tag="PKK85_00570"
     CDS             1877..2074
                     /locus_tag="PKK85_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24550.1"
                     /translation="MKYIETVPLTVHLYPKQREIIEKLQYEIKKEYQVKLPLTELVRI
                     GLERGLPLLLEDTDFILERIG"
     gene            2155..4557
                     /locus_tag="PKK85_00575"
     CDS             2155..4557
                     /locus_tag="PKK85_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24551.1"
                     /translation="MEYWLTQKKRGKKFSFQWIEVKGDKIRALHREYINNNRLDVEHG
                     GRDKNLKKTLHKKTGLPEDEILEYLEELGLFIGENQHLLKVTNKRKGRDKPVGNRDKK
                     EAVEDPVPGYLERALELIKSEDPLQEVTDYIGRNVKHDETLVKLALMVMASAYTPNPL
                     NLALEGPQSEGKTYALVEASKVFPKGDVWNLAGMTPQVLTREHGVLMDRETGESLEPE
                     IKEIKQELNKLSDSKEDKALKNKLKGELQEVYDRGVKVVNMEGKILLFLEAPNTETLA
                     KLRPIMSHDLYEVEYKFVDKAYQNGPQVTMDAKIRGWPVFLYATADKQIGNLWEQIRS
                     RFIVVSPEMTGKKYEAANKFTARKHGHITDPEEIRKEREEFSKCTGYILFLKNTLYQA
                     FFTIHGLTQVPPEKVHFTFNPMAYKLEKSFPATQGQHMRDFKYFMALMDISSLFNLPS
                     RPYLEVRGVPYWMVTMRDLKNVVDVFDKYHFFIKGSELPLRYFTEIIQDLDLTVESIE
                     GDQGFKIKDLRKAFNDKGLPNSDGWIKENVLEPLEDLGLLLKGKAKADKRRKVYTPFK
                     EKYKHLSSNEFLKISFSLDELKEKIEEIKNILTEKGVLGIKFVCKTRYPLQFKKVVRA
                     ILEDEEEDIDSFTKKVHSTYFSVRIIFSDEKTPDKETPSYEEDNLDFENKSLFFKGKE
                     SHKENLEEEIKELQDEALKGSETAREDMEARVLELDNLKRDEKAVSPRSFEDLQVEKY
                     SIPYILEHPDQSDFNIVSRKVTEITGIDEPLVKESLRGLISGGLIRDRGDGNLEAVGG
                     EK"
     gene            4558..5844
                     /locus_tag="PKK85_00580"
     CDS             4558..5844
                     /locus_tag="PKK85_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24552.1"
                     /translation="MTSTTTREELLKNLYGSHFNREIGTPYRQACYHLNILLKKLKAH
                     EGRGDPFYLSVYDYNETARIPLRKPSPRYRYPYEEHVILDKIFFDFDLDYNNRAKEEL
                     KNRATLSSKRDFILKLLEAGRIKEPIKQAKDTARYIQDTYGGEPFLVFSGSKGCHLYI
                     YFNPVSLEHPKETLIRMTRDLRDRGLFNFNTDEEPGLLDTSPVGDVARISRLPGSRHP
                     DTGLYCHPFKLDYSYREIIENSQKPEPPYVEVIPGRMRSTLDKKLLELEQVVKDELEL
                     QRYQRLFSKLEPKRPRQGGAPKIKIKQPEDVLELNRWPCFDKSPYDHDLRVIIANICL
                     WSGLSPEDTARALEVYSQDRGLMEDKHLHHPGEVEKLLQDEEHPRYIFTCRSMKTRGL
                     CRECGGWFYLKLDLGDEFKKRINHYKNGGRGCLSCE"
     gene            5908..6243
                     /locus_tag="PKK85_00585"
     CDS             5908..6243
                     /locus_tag="PKK85_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24553.1"
                     /translation="MNTTKTHCQGCNTPNHNLIELLEVINEKLGVLIYQSNPPVVKEL
                     NTPSGYQVLAITTTTGEVYNHPEDVTGDTWNGTVTFKSKSSMREGLYTVLDGNQIQSL
                     LIKPETGEV"
     gene            6614..>6803
                     /locus_tag="PKK85_00590"
     CDS             6614..>6803
                     /locus_tag="PKK85_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24554.1"
                     /translation="MSGFRLKELQKFNDQLVRMSEYAHNKKIGYEPKCKICNSKNQIK
                     VEAAREEGCTLEEIRDYLE"
BASE COUNT         2168 a         1289 c         1520 g         1826 t
ORIGIN      
        1 ctcccaggta atgacactgg ggatcaggcc accctaaccc ctgcccctca taaaaacctt
       61 tataatctcc tggagcctgg gaaaaagata ggattaaaga aactccagga tctaagcggc
      121 agggacgggc cgggtcttaa aaagttgatc attaacttgg aaaaggaggg aaaactggag
      181 ccctcagggg tgggatactg gagggtgctt gagcctgaga gtatagacct taaaccttaa
      241 tatccttaac aaaataggat tgttagtgtt aaaaaccctt tccaactttt acaaaacttt
      301 acagatcctt tacaaaataa aaataataac tttacgttcc atcccagggg gtgtcataat
      361 tgctccatga aattaagggc cacctcctcc cctttatccc ctggcagacc cctaaaaccc
      421 ctctttttca cttatcttta aacggtttat aaagtatcat agtatcactt atacctccca
      481 taattgaggt agaagtcatt tttatagtgt ttttatttat tgaaagtgta tctttcaagt
      541 aaggagcgaa atgaagatca atatcctcct cttttagatt acttaagcgg tcttcatctt
      601 tccagttact aaattttata ttaacttcat attctacctt ccagggtgtg gagtgagggt
      661 ttctataagt gaaatccaac ccaacaccgt ttaagatata aagaaataag gcttttttaa
      721 tttgttcatt gttaagatat ttctttttaa aaccttctaa atatggtttt aataactcat
      781 cactataagg ggcctctata aatgtaaata gggcactagg agaaatcttg attaaatcta
      841 ataatctttt tttctctata ggctccaggt ttaggttttt atactctttt aatacagctt
      901 ctaccagatc catattaacc agttcataac cataaggaga gtttaataag tggtctccaa
      961 gatacccccg gtaatggggc tcaaaaatag ggttattata ctctttaatt atcttaaaaa
     1021 gtgtatcata atcttttttt agaaagatca ggttttgagc ggggccttta cctggagttt
     1081 tataaggttc tttaatgatt aatccgtcct tctctagacc ttttaatgtc ttggatatct
     1141 ggatcggttt tactggccag tttttagggt cagcgggatc tttagtttca tgatctacat
     1201 aataccagtt tactagtctg catatctctt tttgggatac tttatcttta aaataagctt
     1261 tattttcatt tttattaaat tcccggtatg taatgaggtt atctaagatt agagcccttc
     1321 ttatttcttc tagtgttggt ttcttcatct ttttcctcac tactcccatg aatagtaatt
     1381 gttactagtt atactaatag ttacctaaaa gggtaaaaaa aggagatgtt cacttgacgt
     1441 gttcttatga agcgaaaact gtttatagcc ctaggcccta ctataccata gttacacttg
     1501 aaacctacgt ccctggtgtc tatatattta tagcagaagg atctaagtgt aattaaagaa
     1561 ttgagacggg atttttttta acctaaaatc ccggtaaatc aaataatgaa taaaaagacg
     1621 ttaaggagaa attactaagc tcataaaaga gattaaaaat tagaaatcga ggaatacagg
     1681 gccctggatg gctgcaacct tccagaaacc ccgtaaaact gaaaagttat attttttcac
     1741 cttttcctct ttcacctcta aaagccccta agggcatatt gtaatttgag gatactatat
     1801 taatatactt ttctacatgg ttagggccct aattaaccct ctttttctcc actataaaaa
     1861 atatggaggc tctaccatga aatatatcga gaccgtgccc ctaacggtgc acctataccc
     1921 taagcaacgg gagattattg aaaaactcca atatgaaatt aaaaaggaat accaggttaa
     1981 actcccttta actgagttag tgaggatagg cctggagagg gggctcccct tactccttga
     2041 ggatactgat tttatattgg agcggatcgg atgaacgggc ccttaacatt tagggatctg
     2101 cctgtaattc ttataaaata tggacatagt ttttatttta ctaaaaaggg tggtgtggaa
     2161 tattggctta cccaaaaaaa gagaggtaaa aagtttagtt ttcaatggat cgaggttaaa
     2221 ggggataaga tccgggctct ccaccgggag tatattaata ataaccgttt agacgtggag
     2281 cacggcggca gggataagaa cctcaagaaa accctgcata agaaaacggg actcccagag
     2341 gatgaaatac tggagtatct ggaggagtta ggccttttta taggtgaaaa ccagcacctc
     2401 ctaaaagtta ctaacaagag aaagggcagg gataagccgg tagggaatag ggataaaaaa
     2461 gaagcggtgg aggacccggt ccctggatac ctggagcgag ccctggaatt gattaaatca
     2521 gaggatcctc tccaggaggt aacagattat ataggccgta atgttaaaca tgatgaaacc
     2581 ctggttaaat tagctttaat ggttatggcc tcagcttaca cccctaaccc ccttaattta
     2641 gccctggagg gtcctcagag tgagggtaaa acctatgccc tggtagaggc ctctaaggtc
     2701 tttcctaaag gggatgtttg gaacctggca gggatgaccc ctcaggtttt aacccgggag
     2761 cacggggttt taatggaccg tgagactggg gagagcctgg aacctgagat aaaagagatt
     2821 aaacaagagc ttaataagtt atcggatagt aaggaggata aggcccttaa aaacaagctg
     2881 aaaggggagc tccaggaggt gtatgatcgg ggggttaaag tggttaatat ggagggtaaa
     2941 atcctccttt tcctggaggc acctaatacc gaaacactag ctaaattaag gcctattatg
     3001 agccatgacc tttatgaggt ggagtataaa tttgtagata aagcctacca aaacggtcct
     3061 caggtaacaa tggacgctaa gatcaggggg tggccagttt tcctatatgc cacggcagat
     3121 aaacaaattg gtaacctctg ggagcaaata agaagccgtt ttatagttgt atcccctgag
     3181 atgaccggta agaaatacga ggccgctaac aagttcacgg cccggaaaca tggacatata
     3241 accgaccccg aggagataag aaaggagagg gaggagtttt ctaagtgcac gggttatatt
     3301 ttatttctca aaaatacatt atatcaagct tttttcacta tccacggcct gacacaggta
     3361 ccacccgaaa aggtacactt tacatttaac cctatggctt ataagcttga gaaaagcttt
     3421 ccagctactc aggggcagca tatgagggat tttaaatact tcatggccct tatggatatt
     3481 agttctcttt ttaatttacc ctcccggcct tacctggagg taaggggggt cccgtattgg
     3541 atggtaacca tgagggacct aaaaaacgtg gtggatgtct ttgataaata ccactttttc
     3601 attaaaggat ccgagttacc cttaagatat tttactgaaa taatccagga cctggatctc
     3661 acggtggaga gtatagaggg ggatcagggt tttaagataa aggatttaag aaaagctttt
     3721 aatgataaag gcctccctaa tagtgatggc tggattaaag agaatgtact tgagcccctg
     3781 gaggatttag gcctcctcct taaaggaaaa gctaaggccg ataaaagaag aaaggtatat
     3841 actcctttca aggagaaata taaacattta tcctcaaacg agtttttaaa gatttcattt
     3901 agcttagatg agctaaaaga gaaaatagag gagattaaaa atatcctcac ggagaaaggg
     3961 gtactaggga taaaatttgt atgtaagact agataccccc tgcaatttaa aaaagtagtt
     4021 agggcaattt tagaggatga ggaggaggat atagactcct ttactaaaaa ggtccatagt
     4081 acttactttt ctgtgaggat aattttttct gatgaaaaaa ccccagataa agaaacaccc
     4141 tcctatgagg aggataattt agactttgag aataaaagtt tattttttaa gggtaaagaa
     4201 agccataaag aaaacttaga ggaggagatt aaggagctcc aggatgaggc cctaaaagga
     4261 agtgaaacgg cccgggagga catggaggcc cgggtccttg agttagataa cctaaaacgg
     4321 gatgaaaagg cggttagtcc taggagtttt gaggatctcc aggtggaaaa gtacagtata
     4381 ccctatatac tggagcaccc ggatcaatct gattttaaca ttgtaagccg taaagttacc
     4441 gaaataaccg gtatagatga gcccttggta aaggagagcc taagaggttt aattagtggt
     4501 ggcctgatcc gagaccgtgg agatggtaac ctggaagcgg tgggaggtga aaaataaatg
     4561 acctctacca ccacccggga ggagctgcta aaaaatttat acggatctca ttttaaccgg
     4621 gagataggca ctccttaccg gcaggcctgt tatcatttaa acatattatt gaaaaagtta
     4681 aaggctcatg agggcagagg ggacccgttt tatcttagtg tttatgatta taatgagacg
     4741 gcccggatcc cactaaggaa accctctcct agataccggt atccctatga agaacatgtt
     4801 attttggata aaatcttttt tgactttgac ctggattata ataaccgggc taaggaggag
     4861 ctaaagaacc gggccaccct ttcatctaaa agggatttta ttttaaagct cctggaagcc
     4921 ggcaggatta aggaaccgat aaaacaagct aaggacacgg cccggtatat tcaggatact
     4981 tatggagggg agcctttcct ggttttctca ggatccaagg ggtgccacct ctatatttat
     5041 tttaacccag tatccctgga gcaccctaaa gagaccctaa ttaggatgac acgggattta
     5101 agggaccggg gcctttttaa ttttaatacg gatgaggagc ccggcctcct ggatacttca
     5161 cccgtgggag atgtggcccg tattagcagg ctcccaggga gccggcaccc tgacacgggc
     5221 ctatattgtc acccttttaa attggattat agttaccggg agattattga gaactcccag
     5281 aaaccagagc ccccttacgt ggaggtgatc cctgggagga tgaggagcac ccttgataaa
     5341 aaactcttgg aactggagca ggtggtaaag gatgagctgg agctccaacg ataccagaga
     5401 ctttttagta aattagaacc caaaaggcca cggcagggag gagcacctaa gattaaaata
     5461 aaacaacctg aggatgtgct ggagcttaac cggtggcctt gctttgataa aagcccttat
     5521 gatcatgacc tcagggtaat aatagctaat atatgtctat ggagcggcct atctccagag
     5581 gatacggccc gggccctgga ggtgtactcc caggatcggg ggctcatgga agataagcac
     5641 cttcaccacc cgggagaggt ggagaaactc ctccaggatg aggagcaccc tagatatatt
     5701 ttcacctgcc ggtccatgaa gacccggggc ctatgccgtg aatgtggagg gtggttttac
     5761 cttaagttag acctgggaga tgagtttaaa aaacgcataa accattataa aaatggtggg
     5821 agggggtgtt tatcgtgtga ataaaggcct ttttaaccca tatttcacca tgaggagtga
     5881 taaaaactaa cgggagtata actaataatg aatacaacta aaacccactg ccaggggtgt
     5941 aataccccta accataacct cattgaactt ttagaggtta ttaatgaaaa gttaggggtc
     6001 cttatctacc agagtaaccc tccagtagtt aaggagctaa acaccccctc aggttaccag
     6061 gtcctagcca taaccaccac caccggggag gtttataacc accctgagga cgtgaccggt
     6121 gacacctgga acgggacggt gacatttaaa tctaaaagct caatgagaga gggcctttac
     6181 accgtcctag atggtaacca gatccagagc ctgctaataa aaccagagac gggagaggtt
     6241 taaaacatac tctccccttt atatttttta aatttaattg tatggagaac ccccgttaag
     6301 agttaaaaaa agtttacaaa aaaaaagcac ttttcacgcc aaaaatagag aaaaaatgag
     6361 acttaacacc aaaaaaacgg gtaaaactac caaaaagtaa gcactaacac ctaaggatta
     6421 acacttaaaa accaataacc cctaacccct ggccacccct gagagccccc taaatagtat
     6481 aaaacggctc ttagtaaccg gcagggaata aggtttaaac catcataata gggtggtttg
     6541 tggagttttg acagccaaaa agggggtacc cgttaaagtt aaaaaccgtt aaaggataaa
     6601 tggaggttta tttatgtctg gatttagatt aaaagagctg caaaagttta atgaccaatt
     6661 agtaaggatg agtgagtacg ctcataataa aaagataggt tatgagccta aatgtaaaat
     6721 ctgcaatagt aaaaatcaaa taaaggtaga agcggcccgg gaggaggggt gtaccttgga
     6781 ggagatccgg gattacctgg agg
//
LOCUS       DAOQVW010000015         6729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_12495_length_6729_cov_3.585556, whole genome shotgun sequence.
ACCESSION   DAOQVW010000015 DAOQVW010000000
VERSION     DAOQVW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12495_length_6729_cov_3.585556"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PKK85_00595"
     CDS             <1..413
                     /locus_tag="PKK85_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS24555.1"
                     /translation="AMYFECSNLNITGYDKVMAMIQSLYDFSKDHPDYFRIYCYTGTE
                     RFQMSDNEDAQEIVDLRTGMWRLMVEAIIAGIQDGTIRNDLDPVEMSIYLNILAINAL
                     NLELTSKMVLEARKISQDKFWEDLSRFLEPTIRP"
     gene            908..1753
                     /locus_tag="PKK85_00600"
     CDS             908..1753
                     /locus_tag="PKK85_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24556.1"
                     /translation="MNCKIIYTNSLFIALLSAIAALSGLFRKGLYSQETISMGAQAMG
                     QDLATLLLAIPLLLLSLYLVGQKSLKGQMIWMGSVFYFLYTYASLSFLATYNQLFLVY
                     VAIFSLSLYTFIYGLLSLDASALKHSIKTGNATRVAAIFIIVMATMLAFMWLNMIIGS
                     LLSGSAPAVLETYTTLVVQALDLGVLVPAAIIAGLLLLKRRSWGYVLTSILIVKISLL
                     GTAILSMIYFMAQKGVEVVIGQVLFFVLATVGGIIIAVWFYNKIDSSVYVATTSPKMA
                     LGKGK"
     gene            1753..2253
                     /locus_tag="PKK85_00605"
     CDS             1753..2253
                     /locus_tag="PKK85_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNS24557.1"
                     /translation="MKKDSDQIKREYEDARLEMEKYILVAIFLVQQRWGYIISKELSS
                     DQITTKQWLLMIVLANAFQSPPSLQEMADAMSTTHQNVKQLATRLESRGFLSIERDPN
                     NKRILRLKVTPECSQYWGERTPEDIKSITSLFQGLEDDDVKKLFEVMAKLEKTSGELY
                     WEAKKI"
     gene            2267..2809
                     /locus_tag="PKK85_00610"
     CDS             2267..2809
                     /locus_tag="PKK85_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNS24558.1"
                     /translation="MKVLIVYGTRYGTATEIAEKICLVMESEGLKVDVKDARSLKNFN
                     ISPYQLVVVGSGIKMGKWTKKSLKFLKDNRKELASKMVAIFVTCGAANTEKNRAEGQE
                     KYLNQVAEKYLLNEPRATGLFGSKFDPSAKHGLMYNFTMKAIKKDLEKQGIDTSQPID
                     YRDWEQIRAWARGLAEMVKE"
     gene            2909..3046
                     /locus_tag="PKK85_00615"
     CDS             2909..3046
                     /locus_tag="PKK85_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24559.1"
                     /translation="MDGIIFDVMSYNATRPHTLHPNGTPVGKSLGHDPGLEFNGGETP
                     H"
     gene            3049..3558
                     /locus_tag="PKK85_00620"
     CDS             3049..3558
                     /locus_tag="PKK85_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNS24560.1"
                     /translation="MMHPKTYIPSVVSILRFISAPLFFYTFIIGQFPLSAFIILFAAL
                     TDIMDGCLARRWDAVSTFGAYLDVTADFVLVFLAFFALASAGWYSNCVLGLISLSFFL
                     FLATSRSKKPIYDPMGKYLGSYMMGMVFLSVLFPQTFLRQILLFILVLMVIISTISRL
                     LYLTGRIEI"
     gene            complement(3608..5542)
                     /locus_tag="PKK85_00625"
     CDS             complement(3608..5542)
                     /locus_tag="PKK85_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNS24561.1"
                     /translation="MPENECSSHVESTCSCCGGDLLEEKEESWKHRPLIIISTSAILF
                     TAGIYLENILKLSLPAETLFLGVVVISGYNIVKGALEGVRKIKFNMNLLITIAAVGAF
                     LIGHGEEGAAVFFLFYVAEFLEDYASQRARNSIASLLKLTPQTAQVLRDGIEMEVHVH
                     SVRVDEVVVVRPGDKIPLDGKVVKGVSAVDQASITGESIPVTKKVGNEVFAGTINTEG
                     YLEVQVTRKDTDTVLYRIIELVKESKSRKSRTEKFIDQFATYYTPTVIVLAASVATIP
                     PLILGLNFQDWVYRALVLLVVSCPCALAISTPVSMVSGITAATRKGVLIKGGEYVEEM
                     RKVKAVVFDKTGTLTEGQLEVTDVESVNGYCTKDLLKIAASLESHSKHPIARAITQKG
                     QETGSELCEVHDFKSWTSNGLEGRINDKKFYIGKKSLFSGLEFPQDKLETLEDEGKTV
                     VVVGNENEVIGILGLMDRLRESSRHAVKFLNDNGIKTVMLTGDHEKTARAVAIQLGLE
                     EYHHDLFPADKVNKIEEIVSRYGHVAMVGDGVNDAPALARANIGIAMGAAGSDVAMES
                     ADIALLHDDLSRIEYLVNLSQKTMGVVRQNVALSILIKSSFAILAIFGLVTLWMAVSI
                     GDMGLSLAVILNALRIGSGK"
     gene            complement(5529..5882)
                     /locus_tag="PKK85_00630"
     CDS             complement(5529..5882)
                     /locus_tag="PKK85_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNS24562.1"
                     /translation="MKRNDVCEERCIHQDMVRKVKEKMLEEDVFNHLSRDFKTLGDPT
                     RVKMLFALSLEELCVCDLSSVLGLSESAISHQLRIMRDRNMVKYRREGKMVFYSLSDH
                     HVVQLLKIGVEHARE"
     gene            5968..6051
                     /locus_tag="PKK85_00635"
     tRNA            5968..6051
                     /locus_tag="PKK85_00635"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6002..6004,aa:Ser,seq:cga)
     gene            complement(6095..>6729)
                     /locus_tag="PKK85_00640"
     CDS             complement(6095..>6729)
                     /locus_tag="PKK85_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS24563.1"
                     /translation="EEKLASYPEKTTIFVLINGQVEAALLMSDLIRPESQEAISGLKD
                     MGIKSIMITGDRMEVARWVAEELGLDQYFAEVLPGEKAKKIRQIQEEGLVVAMTGDGI
                     NDAPALAQADVGIAIGAGTDVAIEAADIILVRNNPLDALYIIRLARSTYQKMVENLAW
                     GAGYNIFAIPLAAGVLYAYGIILTPAMGAVLMSASTIIVAVNSRFLRISK"
BASE COUNT         1820 a         1529 c         1456 g         1924 t
ORIGIN      
        1 atgccatgta ttttgagtgt tcaaatctta atattaccgg ctatgataaa gtgatggcta
       61 tgatccaatc actctatgat ttttccaagg atcaccccga ttacttccgt atatactgtt
      121 acactggaac tgaaaggttt caaatgagtg ataatgaaga tgcacaagaa attgttgatt
      181 tgaggactgg aatgtggagg cttatggttg aagccataat cgcaggcata caggacggaa
      241 ccattcgcaa tgatttggat ccggtagaaa tgtctattta tcttaatatt ctagctataa
      301 atgctttgaa tctggaatta acatctaaaa tggtactaga agcccgaaaa atttctcaag
      361 ataagttttg ggaggatctg tcccgttttt tggaacccac aatcagacct taatttgaaa
      421 aaaatgtgat ggaggttgat ataaactgtg attaaatcaa tttgaatagt tttcacacct
      481 ttttagactt cttaagatta tgacagttgt caccaccttc cattgtagtt gatttgataa
      541 gacttgacgg gttcttgttt tcccgttgga taaatgggat tttttccttg taaagatgtg
      601 ccatcaatct tggtgttgta tttcaaaata ggtacacact tgctgcagtg ctttggcctt
      661 ggtgtttagg aagtagttgt catgttttga ataatgttta ccagtcaaaa ctctgatctg
      721 ggcgaaaatt atggatgaaa aacttttaat tttattgata atccctattt tcgaaaaatt
      781 atcaggaagt cttgttttaa aattgggata aatggattgc tgatcccaaa cttttatata
      841 gcactcccca aatattaggt aatatattac ataattaagt aatatattac caatggagtt
      901 gatgaaaatg aactgtaaaa taatttacac aaattccctg tttatagctc tattatcagc
      961 tatcgctgcg ttatccggac ttttcaggaa gggattatac tcccaagaaa cgatttccat
     1021 gggagctcaa gccatgggtc aggatctggc cactctgctg ctagctatcc ccctgctctt
     1081 actttctttg taccttgtag gccagaaatc tctaaaaggc cagatgatat ggatggggag
     1141 cgtattctac tttttataca cctatgcatc cctttccttc ctggctacct acaaccaact
     1201 atttctggtt tacgtagcca tcttcagctt gtctttgtac accttcatct acggacttct
     1261 gtccctggat gctagtgccc ttaaacacag tattaaaact ggaaatgcca ctagggtagc
     1321 cgcaatattc atcattgtca tggcgactat gctggctttc atgtggctta atatgatcat
     1381 cggatccctg ctatccggat ccgcccctgc agttctggaa acttacacca ccctggtagt
     1441 tcaggccctg gatctgggag tgctggtacc tgctgccatt attgcaggct tactcctctt
     1501 aaaaaggcga tcttggggtt atgtgttgac atctatctta atcgtcaaga tatccctact
     1561 gggaactgct attctctcca tgatatattt catggctcag aagggggtgg aagtagtcat
     1621 aggccaggtc ctcttcttcg tgttagccac ggttggggga attataattg ctgtatggtt
     1681 ctataataaa atagatagct cagtatatgt agccacaacc tcgccgaaaa tggccttggg
     1741 gaaagggaaa taatgaaaaa agatagtgac caaatcaaga gggaatatga agatgcaaga
     1801 ttagaaatgg aaaaatacat actggtggct attttcctgg tgcagcagcg ttggggttac
     1861 atcatcagca aagagctgtc cagtgaccag atcaccacca aacagtggtt gttgatgata
     1921 gtactggcca acgccttcca gagcccccct tccctgcagg aaatggctga tgccatgagc
     1981 accacccatc agaatgttaa acaactggct acccgtttgg agtctcgtgg gttcctcagt
     2041 atagaacgtg accctaacaa caaacgcatc ctccgtttaa aggtcacacc agaatgttcc
     2101 cagtactggg gggaaagaac cccggaggac ataaaatcca taacctccct attccaggga
     2161 ttggaagacg atgatgtgaa gaaactgttt gaagttatgg ctaaactgga gaagacttct
     2221 ggagaactgt actgggaagc taaaaaaata tgaagactga tagatcatga aagttttaat
     2281 tgtatacggg actagatacg gtactgccac agaaatagca gaaaaaatat gcctggtgat
     2341 ggaaagtgaa gggttaaaag tagatgttaa ggatgcccgg agtctgaaga acttcaacat
     2401 ctccccctac caattggtag tggtgggcag tggaataaag atgggaaaat ggactaaaaa
     2461 atcccttaaa ttcctgaaag ataaccggaa ggaactggcc agtaaaatgg tggccatctt
     2521 tgtcacctgc ggcgcagcca atacggaaaa aaaccgggct gaaggccagg aaaagtactt
     2581 gaatcaagtg gcggagaagt acctcttaaa tgaaccgagg gccaccggtc tttttggcag
     2641 caaatttgat ccaagcgcta aacacggttt gatgtacaat ttcaccatga aagccattaa
     2701 aaaggaccta gaaaagcagg gaatcgacac ttctcaaccc attgattacc gtgactggga
     2761 acagatcaga gcttgggcac ggggactggc ggaaatggtc aaggaatgat cagtggtcaa
     2821 aataaagatg atgtgtaaat aacttgaaaa gcatcgctga aatagtagga gtaggtttaa
     2881 ttgttggatg gtgttagcat ttgcatcaat ggacgggata atcttcgatg ttatgagcta
     2941 caacgccacc aggccccata cccttcaccc caacggtaca ccagtgggca aatcactggg
     3001 tcatgatcct ggcctagagt ttaatggtgg ggaaactccc cactaattat gatgcaccct
     3061 aaaacgtata ttccctcggt agtgtccata ttacggttca tatctgctcc ccttttcttt
     3121 tacacattca taattggaca gtttccacta tcagcattca taatcctctt cgctgctcta
     3181 actgacatca tggatggttg cttggcccgg agatgggatg cagtttccac cttcggtgct
     3241 tatctggatg taactgctga ttttgtcctg gtttttttgg cattttttgc actggccagt
     3301 gctggctggt attctaactg tgtactggga cttatcagcc tgagtttttt cctattccta
     3361 gccacctctc gttctaaaaa accgatttat gatcccatgg ggaaatattt gggttcctac
     3421 atgatgggga tggttttttt atcggttttg tttccacaaa cttttttgag gcagatactg
     3481 ctcttcatac tggtattaat ggttataata tcaactataa gtcgtctatt atatcttact
     3541 ggtcgaattg agatttgaaa ggttaaaatc attttcaaag gtttttgcta gtgattacaa
     3601 tatttgacta ttttccgctg ccaattcgta gtgcatttaa tataactgcc aaactaaggc
     3661 ccatgtctcc aatacttaca gccatccata gagtaactaa tccaaagatt gccaggatgg
     3721 cgaatgaact tttgatcagt atggaaaggg ccacattctg cctgaccacc cccatggtct
     3781 tctggctgag attaaccaga tattctattc tggacagatc atcatgtagg agggcgatgt
     3841 cggcactttc catggctaca tctgaacctg cagcacccat tgctatccca atgttggccc
     3901 gggccagtgc cggtgcatcg ttgacaccat ctcccaccat ggccacgtgt ccatagcggg
     3961 agactatttc ttcaatttta tttaccttat ctgccgggaa caagtcatga tggtactcct
     4021 ccaagcccaa ctggatagcc actgcccggg ctgttttttc atggtctcca gtgagcataa
     4081 ccgtttttat gccattgtca tttaaaaatt tcaccgcatg ccgggaactc tcccgaagac
     4141 ggtccatcag tcccagaata ccaatgactt cattttcatt ccccaccaca accacggttt
     4201 tgccctcatc ctccagggtt tctaatttat cctggggaaa ctctaaacct gaaaagaggc
     4261 ttttcttccc gatgtagaac tttttatcat ttatccttcc ttccaaacca ttactagtcc
     4321 atgatttgaa atcatgaacc tcacaaagtt cagaaccggt ttcttgtccc ttttgggtaa
     4381 tggccctggc aatgggatgt ttggaatgtg attctaggga tgccgcgatt tttaaaagat
     4441 ccttggtgca gtaaccattt acactctcca catcggtgac ttccagctgt ccttcggtga
     4501 gggttccggt cttgtcaaag accactgctt taactttacg catttcttcc acatattctc
     4561 caccttttat gagcacacct ttcctggtgg ctgctgtgat tccagatacc atggatactg
     4621 gtgtggatat ggccagggca cagggacatg atactaccag gagaaccagc gcccggtaga
     4681 cccaatcctg gaaatttaat cccaaaatta gtggggggat agtggccacg gatgcagcta
     4741 gcacaatgac cgtgggagtg taataggtgg caaactggtc aatgaacttc tctgtcctgg
     4801 atttcctgct cttagactct ttcaccagct ctataatcct gtaaagaact gtatctgtgt
     4861 ctttacgggt cacctggact tccaaatagc cttcggtgtt aatggttcct gcgaagactt
     4921 catttcccac ctttttagtg actggaatac tttcaccggt tatggaagct tgatccacgg
     4981 cagacacccc tttaacaacc tttccatcca ggggtatttt atctcctggg cgcaccacca
     5041 ccacttcatc cacccgaaca ctgtgcacat gaacctccat ttcaatgcca tcccggagta
     5101 cctgggcagt ttgaggggtc agtttcaaga gggatgctat ggaatttcgg gcccgttggc
     5161 tggcgtaatc ttccagaaac tcagccacgt agaacaagaa aaatacggca gcaccttctt
     5221 ctccatggcc tattaggaat gcacctaccg cggcgatggt gatgagaaga ttcatgttga
     5281 atttgatctt ccgcacgcct tccagggcac ccttaacaat gttgtaccct gaaattacca
     5341 ccactcctaa aaatagtgtc tccgcgggta aactcagttt gaggatgttc tctagatata
     5401 taccggcggt gaagaggatg gctgaggtgg aaattattat aagtggcctg tgtttccagg
     5461 attcttcctt ttcttcaagg agatcccctc cacagcaact gcaagtactc tccacatggg
     5521 aagaacattc attctcgggc atgttctaca cctatcttca aaagctgaac cacgtggtga
     5581 tcggataaag agtaaaatac catttttccc tctcgacggt acttaaccat gttacgatcc
     5641 ctcataatcc ggagttgatg ggagatggct gattcactaa ggcccagcac actggacaga
     5701 tcacagacgc acaactcctc cagagagagt gcgaacagca ttttcaccct tgtggggtcg
     5761 cccagggtct taaaatccct tgataaatgg ttgaaaacat cttcctcaag cattttctct
     5821 ttgactttgc ggaccatgtc ctggtggatg cagcgttctt cacagacatc attccttttc
     5881 atatgaatag atgctcatat gttcatataa agtttgctat cctaaactta taagaaagtg
     5941 gaagttaaca tagaatagat acatcatgcc gggatagtct agtcaggtaa ggcgtaggat
     6001 tcgaaatcct atgagcgatg ctcaccctgg gttcaaatcc cagtcccggc gcttttcatc
     6061 taccaaatac caactataag ctgcaattga tccttcactt gctgattctc aaaaaccggg
     6121 agttaacagc cacgatgata gtgctggccg acataagaac cgcccccatg gcgggggtga
     6181 ggattatacc gtaggcatac aacactccag ctgccagggg gattgcaaag atgttgtaac
     6241 cagcccccca ggccagattc tccaccatct tctggtaggt ggagcgggcc aggcggatga
     6301 tgtacaaggc gtctaatggg ttgttacgta ccaggataat gtctgcagct tcgatggcca
     6361 catcagtacc agcgccgatg gctattccaa catcagcttg ggccaaggca ggggcatcgt
     6421 taatcccatc tccagtcatg gccaccacca agccttcttc ctgtatctgt ctaattttct
     6481 tagccttctc ccctggaaga acctcggcga agtactgatc cagacccaac tcctcagcca
     6541 cccaccgtgc cacttccatt cggtccccgg tgatcatgat ggatttgatt cccatatcct
     6601 tcaaacccga aatagcttcc tgggactcgg gacggatgag atcagacatc aaaagggcgg
     6661 cttctacttg tccattgatc aacacgaaga tggttgtttt ttctggataa gaagccagtt
     6721 tttcctctg
//
LOCUS       DAOQVW010000016         6635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_12729_length_6635_cov_3.502736, whole genome shotgun sequence.
ACCESSION   DAOQVW010000016 DAOQVW010000000
VERSION     DAOQVW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12729_length_6635_cov_3.502736"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKK85_00645"
     CDS             complement(<1..885)
                     /locus_tag="PKK85_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019263114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNS24564.1"
                     /translation="MKGSRAIIQSLTDQGVDVVFGYPGGVLLPLYDEIYDSDLRHILV
                     RHEQCAAHAADGFARASGKVGVCIGTSGPGATNLVTGIATAYMDSSPVVALAGQVASH
                     LIGNDAFQEVDTLGLTMPITKHSFQPMTPTEIPSMIKSAFYIASTGRPGPVVLDLPKD
                     VQEAELEYPDKITIDLPGYKPTKKGHPLQVKRAAEVILNAKKPVILAGGGVILSGSSD
                     EIFKLSELLGAPVTTTLMGKGCFPEDNPLSLGMLGMHGRKVSNFLVDDCDCLVAVGCR
                     FSDRTTGDVSKFAVNAKII"
     gene            1044..2360
                     /gene="purD"
                     /locus_tag="PKK85_00650"
     CDS             1044..2360
                     /gene="purD"
                     /locus_tag="PKK85_00650"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNS24565.1"
                     /translation="MKILVVGTGAREHVICQVVSQNAELYSYMSNQNPGIAKLAEKFY
                     IGNEMELEEVKKQAISWGVDLAIIGPESPLGEGIVDLLLEEGIACVGPTQAAARIETD
                     KAFMRKLFIDHGIDGSVTYGVFDDVEEAGHFIDDFGKELVVKPVGLTGGKGVKIVGEH
                     LKDDEDAKKYVKEIINNKISGYASVVLEERLVGEEYTIQALVDGNRVIPMPAAQDHPH
                     AYEGDQGPITGGMGSYSDTNGLLPFLDEKAYQDSVKIMEDTIKAVRKEVGPYKGILYG
                     QFMLCRDGPRLVEYNARFGDPEAMNVLPLLKTSFVELCQQVVDGNLKKASFEPKATVC
                     KYLVPNGYPESGRANSIIRVDEEKINEAGAGVYYAAVNQKNNQIFTSSSRALALVAAG
                     ESISEAEEICEKATSFVDGDLYHRRDVGTTALIQKRIKHMEDLLGT"
     gene            2404..3309
                     /gene="argF"
                     /locus_tag="PKK85_00655"
     CDS             2404..3309
                     /gene="argF"
                     /locus_tag="PKK85_00655"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNS24566.1"
                     /translation="MQHVLSVLDIQDQFRDILRQAQIFKKDHGEDEPLKGKTLAMIFE
                     KSSTRTRVSFEVAMYQLGGFPLYLSTSDLQLGRGELIEDTSRAMTRYVDGVMIRAREH
                     QDVVKFSIHSRVPVINGLTNLEHPCQALADMLTILEWKGTFNVKMVYVGDGNNVVNSL
                     LLAAAVLGMDFTVACPPSYEPRPEIIKKALKIALKTGSNLEVTHQLQEAVKNADVLYT
                     DVWVSMGDEETAEQRLKDLQAYQINTELLELANPEAMVLHCLPAVRGQEITEEVLNSP
                     QSAVWDQAENRLHVQKALLYLLLKD"
     gene            complement(3334..3879)
                     /locus_tag="PKK85_00660"
     CDS             complement(3334..3879)
                     /locus_tag="PKK85_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS24567.1"
                     /translation="MDKTEKEILDAALKVFAEKGYDGAKTKLIAKESGFTEMTLFRKF
                     GTKENLFHQVLMGNYHGITSDLVQLFSQPMDQSQDPLRRLIMGIIEMVDKNYDYVKIL
                     ITEDHGTSEKVINYGINNLEKFLENVSPEAEIDYRVLAFNIMSFSYFIIFNKRNGDPF
                     VDFDAAVEEFITCMASCLKID"
     gene            complement(3939..5630)
                     /gene="argS"
                     /locus_tag="PKK85_00665"
     CDS             complement(3939..5630)
                     /gene="argS"
                     /locus_tag="PKK85_00665"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNS24568.1"
                     /translation="MYRKLENQAAKSVQAAMKKLQWNVAVNIKFEQPPSPDMGDLATN
                     MAFQLAGVLKKSPAAIAENILGNLEVPPLIARVETRGPYLNFFIDNAKFFQEVLESVD
                     EGYGHLELQEDSVILEHTSANPNGPLHIGHIRNAIIGDSLARILRAGGYPLETQYYVN
                     DMGRQIAMIVWGLQNLDYQLNPEEKSDVEMGKLYFQVNEALKANPEIKREIDKILKIY
                     EQEFPPQLEKLFNLVVDECLEGVKETSQRLHIHHDKFVRESQFIREGRVDTILDELKV
                     YTKENEVLYLDLNQFGIEKELILRRSDGTSLYSTRDLAYHEQKAEEANISIDILGSDH
                     KLALEQLSVALELIGCPRPEVIFYEFITLPEGSMSTRRGVFISVDDLLDEAVKRAMNE
                     LIERRPELVGRERKKIAEIIGVGAIRFYIARLSPEKHVVFKWDEALSFERGCASIQYA
                     HARACKLLEKANYSHVQVELDEDGLNVLEEMEMDLIKTIARFTRVIEESAEARRVHPV
                     AQYALELAGVFNRFYKSIPVLGSDAEDLRLMLVDKSRITIRNSLALLGIESPASM"
     gene            complement(5688..6176)
                     /locus_tag="PKK85_00670"
     CDS             complement(5688..6176)
                     /locus_tag="PKK85_00670"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNS24569.1"
                     /translation="MSKRRKTPRKSRDDHLEDEFGHSTRREIVSYLLIIVVGILLAQH
                     LNVVVSGSMEPVFYRGDVVLIEKSNFMGIEEINPQTLQVGDIIIYRATWYPEPVIHRI
                     IRIEKDFQGRSYYITKGDNNDAADPAPVYPEQVQSKVLSWGQNPLFIPKIGYLTLWIR
                     GL"
     gene            complement(6186..>6635)
                     /locus_tag="PKK85_00675"
     CDS             complement(6186..>6635)
                     /locus_tag="PKK85_00675"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNS24570.1"
                     /translation="PAKVYNSEEECVKAIYQGAVKEGDVVVIRYEGPQGGPGMREMLN
                     PTSAIFGLDIKKVALITDGRFSGGTRGPCIGHVSPEAQAGGPIAVVEDGDMIRIDMPR
                     REIELLVSPEEIEQRLGKVVHPQKEVKGWLARYRKLATSANEGAVLR"
BASE COUNT         1773 a         1543 c         1465 g         1854 t
ORIGIN      
        1 gataatcttg gcattgacag caaatttaga cacgtccccg gtggttcgat ctgagaaacg
       61 acatcccacc gcgactaggc agtcacagtc atccaccagg aagttggata ctttgcggcc
      121 atgcatcccc aacatgccca gtgacagcgg gttatcctca gggaagcacc ctttacccat
      181 taaagtagtt gtaactggtg caccaaggag ttctgaaagt ttaaaaattt catcagaaga
      241 tccagatagt atcaccccgc caccagccag tatcactggt tttttagcat ttaatatcac
      301 ttcagcggcc cgtttaacct gtaaaggatg tcccttcttg gtgggcttgt aaccgggtaa
      361 atcaatagtg atcttatcag gatattccag ctcagcttcc tgaacatctt tgggaagatc
      421 cagaaccact ggcccggggc gtccggtaga ggcgatgtag aatgctgatt taatcatgga
      481 tggtatttca gtgggggtca tgggctggaa actgtgtttg gtgatgggca ttgtaagtcc
      541 cagggtgtcc acctcctgaa atgcatcatt cccaattaaa tgactggcaa cttgcccagc
      601 tagagctact actggggatg agtccatgta agcggtggct atcccagtaa ccaggttggt
      661 ggctccgggg ccggatgttc cgatacatac cccgactttt cctgatgccc gtgcaaaacc
      721 atcggcagcg tgtgctgcgc actgttcatg cctcaccagt atgtgcctaa ggtcagaatc
      781 atatatctca tcatataacg gaagtaacac tcctccgggg tatccgaaga caacatccac
      841 tccctggtcg gtaagtgatt ggattatggc tctactgccc ttcataaaaa caccttaact
      901 aactaaacat taatagtatc ccactcataa acactttctc tcctccacac tttaatcttg
      961 cgatgccttg gtggagaggg aatgggtagt acatccaatt tttataagat catggattta
     1021 tagatgcatg ggagggctag tctatgaaaa tattagtagt gggaaccggc gctcgagaac
     1081 acgtcatttg ccaagtggtt agtcaaaacg cagaattata ttcatatatg agtaaccaga
     1141 acccaggaat agcaaaatta gctgaaaaat tctatattgg aaatgaaatg gaattagaag
     1201 aggttaaaaa acaggccatt tcatgggggg tggatctggc tataataggg cctgaatctc
     1261 ccttgggaga aggtattgtg gatttactgc tggaagaagg gatcgcttgt gtggggccca
     1321 cccaggctgc ggcccgcatc gaaactgata aggcttttat gaggaaactc ttcatagatc
     1381 acggtattga tggatcagta acttatgggg tgtttgatga tgtggaagaa gctggacact
     1441 tcattgacga ttttggaaaa gagttagtgg tgaaaccagt tggattaacc ggtggtaaag
     1501 gagttaaaat cgtgggagaa caccttaaag atgatgaaga cgctaaaaaa tatgttaaag
     1561 agattattaa caacaaaatc agtggttatg ccagcgtagt cctggaggaa cgcttggtgg
     1621 gggaagaata cactattcaa gccctggttg atgggaatcg tgtcatcccc atgccggctg
     1681 cccaggatca tccccatgca tatgaagggg accagggacc cataacaggg ggaatgggat
     1741 cttactctga caccaatgga ttgctgcctt ttttggatga aaaagcatac caagattctg
     1801 ttaaaatcat ggaagatact atcaaagccg ttagaaaaga agttggaccc tataaaggca
     1861 ttctttacgg gcagttcatg ctctgccggg atggaccgcg cctggtggag tacaacgctc
     1921 gattcggaga ccccgaggcc atgaacgtat tacccctttt aaaaacgagt tttgtggaat
     1981 tatgtcaaca agtggttgat ggtaatctga aaaaagcatc ttttgaacct aaagccacgg
     2041 tttgcaagta tttagtgcca aatggctacc cagagagtgg tagggctaac tccatcatca
     2101 gggtagatga ggaaaaaata aacgaagcag gagccggggt ttattacgcc gctgttaacc
     2161 agaaaaacaa ccaaatattc acatcatcct cacgggccct ggccctggtt gctgcaggag
     2221 aatcaatttc cgaagcagaa gagatatgtg aaaaggccac cagcttcgtg gatggtgatc
     2281 tttaccatcg tcgggacgtg gggaccacgg ctctcatcca aaaacgaata aaacacatgg
     2341 aagacttatt gggaacttag aactatacta attaacaacc agtgaattct cggagatgag
     2401 ataatgcaac atgttttatc agtgctggac atccaggacc agttcaggga cattctcaga
     2461 caagcccaga tatttaaaaa ggaccatggt gaagatgaac ctttgaaagg taaaacactg
     2521 gccatgatat ttgaaaaatc ctcaacaaga acacgtgttt cctttgaggt agccatgtac
     2581 caattaggag gcttcccact ctacctgtcc acctcggact tacaacttgg ccggggggaa
     2641 ttgattgagg acacttcccg ggccatgacc cgctacgtgg acggggtgat gatccgtgcc
     2701 cgggagcacc aggatgtggt taaattttcc atacactcca gagttcccgt tattaacggc
     2761 ctcaccaatc tggaacaccc ctgccaagcc ttggctgata tgttaaccat actggaatgg
     2821 aagggaacct tcaacgtcaa aatggtttat gtaggggatg gtaacaacgt ggttaactca
     2881 ctgttgctgg ctgctgctgt tctggggatg gacttcactg tggcctgccc accaagctac
     2941 gaaccccgcc ccgaaattat taaaaaagcg ttgaagatag ctttaaagac tggatctaat
     3001 ctggaagtta ctcatcaatt acaagaagca gttaaaaatg cggatgttct ttacactgat
     3061 gtatgggtca gtatgggtga tgaagaaacg gcagaacaac gtttaaaaga tcttcaggct
     3121 taccaaatca acactgaact tctggaactg gctaatccag aagccatggt cctgcactgc
     3181 cttccagcag tacgtggaca ggaaataact gaggaagtcc ttaacagccc ccaatccgca
     3241 gtttgggatc aggcagagaa tcgtttgcac gtgcaaaagg ctcttcttta ccttctcttg
     3301 aaggattaag tctggatgta tttttgtcta tctctaatca atcttcaagc agctagccat
     3361 gcatgttatg aactcttcaa ccgcggcatc aaagtccaca aagggatctc cattgcgttt
     3421 gttaaatatg atgaaatacg aaaatgacat tatgttgaaa gccaacaccc tataatctat
     3481 ttcagcttcg ggggatacat tttctagaaa tttttcgagg ttgttgattc catagttaat
     3541 caccttctcg gaggtaccat gatcttcggt tatgagaatt ttgacataat catagttttt
     3601 atccaccatc tctatgatgc ccattattag ccgtcttagt gggtcctggg attgatccat
     3661 aggttgactg aatagctgga ctaggtcgct ggtaatacca tgataattcc ccattaaaac
     3721 ctggtggaaa agattttctt tggtaccaaa ctttctaaag agggtcatct cggtgaagcc
     3781 agattccttg gcaatgagtt tagttttagc accatcatat cccttctctg cgaagacttt
     3841 taatgcagca tctaaaatct ctttttctgt cttatccatt ttcttcctct tctgatacca
     3901 tttatttaaa taaacatcga taaattctgt cctgaaacct acatactggc gggtgattcg
     3961 atgcccagca gtgccaggga atttcgaatg gtaatccttg atttatccac taacattaaa
     4021 cgtaaatctt ctgcatctga tcctaaaact ggaattgact tgtagaaccg gttgaagact
     4081 ccggccagtt ccagtgcgta ctgggctacg ggatgaactc tacgcgcctc tgctgactcc
     4141 tctatgacac gcgtgaacct ggctatggtt ttaattagat ccatctccat ctcttccaga
     4201 acattcaatc catcttcatc tagttctact tggacatggg aataattggc tttttccaat
     4261 agcttacagg cccgggcatg ggcatactgg atggaagcac aaccccgttc gaaactcaat
     4321 gcctcatccc acttgaaaac aacatgtttc tccggtgaca atcgggctat ataaaaccgg
     4381 atggcaccca ccccaattat ttcggcaatt ttttttcttt ctctcccaac caactctggt
     4441 cttctttcta taagctcatt catagctcgt ttcacagctt catccaataa atcatccacg
     4501 ctgatgaaca ctcccctcct ggtggacatg gacccttcag ggagggtgat gaattcgtag
     4561 aaaataactt ctggccgggg acacccgatt agctctagcg ccacacttaa ctgttccagt
     4621 gccagtttat ggtcagatcc caggatatca atggatatat tagcttcttc tgctttctgt
     4681 tcatggtagg ctaaatcgcg ggtggagtaa agtgatgtac catcggaacg acgcagtatc
     4741 agctcttttt ctatgccgaa ttggttcaga tccaagtaga gaacctcatt ttccttggta
     4801 taaaccttca attcatctaa aatggtatct actctaccct ctcgtatgaa ctgactttcc
     4861 cgcacgaatt tatcgtgatg tatgtggagt ctctgggaag tttccttcac cccctccagg
     4921 cattcatcca ccaccagatt gaaaagtttt tccagttgtg ggggaaattc ctgttcatag
     4981 attttcagta tcttatcaat ttctctcttg atttctggat tggcctttaa agcctcattc
     5041 acctgaaagt acagttttcc catctctaca tctgattttt cttctggatt caactggtaa
     5101 tccagatttt gcagccccca tacaatcatg gcaatctgcc ggcccatatc gttaacgtag
     5161 tactgcgttt ccaggggata acccccggcc ctgagtatcc tggccagtga atccccaatg
     5221 attgcatttc ttatgtgccc aatatgtaag ggtccgttgg gatttgctga agtgtgttcc
     5281 agaatcactg aatcttcctg aagttctaga tgaccatatc cttcatccac cgattctaga
     5341 acctcctgga aaaactttgc attgtcaatg aagaagttaa gataagggcc ccgagtttct
     5401 acccgggcaa tgaggggtgg tacttctagg ttccccagga tgttttctgc tatagctgca
     5461 ggagatttct ttagcacccc cgccagttgg aaggccatgt tagttgctaa atcacccata
     5521 tctgggctgg ggggttgttc gaatttaata ttaaccgcca cattccattg gagcttcttc
     5581 atggcggctt gcactgattt tgcagcctga ttttccaatt ttctgtacat aaattcacct
     5641 agtaaataaa aaaaattcga tacttaaata gaacgtgaaa atgaagacta gagccccctg
     5701 atccacaggg ttaggtagcc gatttttggg atgaagagtg gattctgccc ccaactcagc
     5761 accttggatt gcacctgttc tgggtacacc ggggcggggt ctgctgcgtc attattgtca
     5821 cctttggtaa tgtaataact gcgtccctgg aaatcttttt cgatgcgtat tattcggtgt
     5881 ataactggtt cagggtacca tgtagccctg tagataatta tatctcctac ttggagagtt
     5941 tggggattta tctcttctat ccccatgaaa ttgctttttt caattaacac cacatccccc
     6001 cggtagaaaa caggttccat actgccggac accaccacgt tcaggtgctg ggccagcaga
     6061 atccccacta ctatgatgag caagtaactg acaatttctc gcctggttga atggccaaac
     6121 tcatcttcca gatgatcatc cctacttttc cgaggagttt tccttctttt ggacattaaa
     6181 cacacctatc tcaggacagc tccttcatta gctgaagtag ccagtttacg gtacctggcc
     6241 aaccatcctt taacctcttt ctggggatga actacctttc ccagccgttg ttcaatttct
     6301 tcaggggaca ctagaagttc aatttcccgg cgtggcatgt ctatccggat catatcccca
     6361 tcctccacca ctgctatagg gccaccggcc tgggcttcag gtgaaacgtg gccgatacag
     6421 ggccctcggg ttcccccaga aaatcttcca tcggttatga gtgctacttt ctttatatct
     6481 aggccaaata tggctgaagt tggattgagc atctccctca tcccgggtcc tccctgtggt
     6541 ccctcgtagc ggatgactac cacgtctccc tcctttactg caccctggta tatggctttc
     6601 acacattctt cttctgaatt atagaccttg gctgg
//
LOCUS       DAOQVW010000017         6428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_13289_length_6428_cov_3.415660, whole genome shotgun sequence.
ACCESSION   DAOQVW010000017 DAOQVW010000000
VERSION     DAOQVW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13289_length_6428_cov_3.415660"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /gene="tsaA"
                     /locus_tag="PKK85_00680"
     CDS             <1..238
                     /gene="tsaA"
                     /locus_tag="PKK85_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNS24571.1"
                     /translation="IYWFDRAEDPLMKVVPHGRTKKRGIFSTRAPPRPNPIGLTMVDL
                     IKREGPVLTVRGLDALDGSPVLDIKPYLPDLDSV"
     gene            complement(252..1943)
                     /locus_tag="PKK85_00685"
     CDS             complement(252..1943)
                     /locus_tag="PKK85_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HNS24572.1"
                     /translation="MKDDHYLARILGRIHGRGYKAYQDLRGSYQFNQFTLHVDHVQRD
                     PYASPSHLRVEVEDHHYPPHFFSTSIRRVALEDYIARSFQRSIHAHLTRPRGSGRSGV
                     MRIDAGGQEILERSCVNINPENLEIRFTLGLSAKGRKIMGEDTKKILVEILPHIVDDS
                     CFFTGNPEVESHLHLAEDAQAIREELYIEGLVAFIANGSTLPRRSGICSQPLSGAVPF
                     KSPESLRISLNSPNQGLITGMGIPEGVTLIVGGGYHGKSTLLQAVEAGVYNHLPGDGR
                     ELVVTRESAVKIRAEDGRSIQKTDINSFIHEPPFMKSTKEFSTQNASGSTSQAANIVE
                     ALEAGSQLLLFDEDTSATNFMIRDERMQALVKSNQEPITPYLDRVQELYDQEGVSTVM
                     VMGGSGDYFEVAHTIIMMDCYQPHNRTLEARKVAQQIPTGRSREVPYPFSYRHRTPLK
                     ESINPYRGRRMKISNRGKDTILLGSDTIDLSQVKQLVDPSQTRALAYALYHAQKYMDG
                     HSTLREILSRLEADLERKGLDVLSPPGRENPPDLAGFRPFELAAALNRLRSLSIK"
     gene            complement(1998..2606)
                     /gene="csa3"
                     /locus_tag="PKK85_00690"
     CDS             complement(1998..2606)
                     /gene="csa3"
                     /locus_tag="PKK85_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated CARF protein Csa3"
                     /protein_id="HNS24573.1"
                     /translation="MGCVNIENTLISTIYSLEPVMACITRFSPKKLILLLEEDAPEKK
                     LEAERMLIETVGRVMEITTQLTSIYEVVKIAQDTAEVIEAENAQGRMVVVNISGGRKP
                     QALGALFGCYARHKMVERIVYVTEEDGEVVDLPILNFGISKTKLMVLEELKKGDTSVK
                     NLAQKIGISRGMTYNHIRELREMGFIDSQQLEITSAGELAII"
     gene            complement(2895..3449)
                     /locus_tag="PKK85_00695"
     CDS             complement(2895..3449)
                     /locus_tag="PKK85_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNS24574.1"
                     /translation="MDLLAILLLAVGLAMDAFSVSITRGLSVRCDIKHALTIALLFGG
                     FQALMPVLGWASGRELASFMNQWAPWVAFTLLVLIGVKMIYEGFHHPEEEEACKLLSL
                     RELLFLSVATSIDAFAVGVTFAFLNISLLQPIIIIGLVTFLLSLVGVYIGKQVGHLFE
                     GKMELVGGIILIAIGCKILLENML"
     gene            3607..4179
                     /locus_tag="PKK85_00700"
     CDS             3607..4179
                     /locus_tag="PKK85_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS24575.1"
                     /translation="MYWFRRGNQLMKAGFYSDAVLAYDRALELDPDNSFVWDNRGVAL
                     SHLGLVEESQESFQLALDYDPSNAQAWSNLGVALASQYRLEEALNSLDQAVKLDESND
                     EAWNNRGTALLALKKYREAVESFDKALEINSKNPQAWAGKGSSLGFLKQYQEAIECME
                     RFIDLASALDSPQVEEAWAMILEWKIRIKG"
     gene            complement(4432..4791)
                     /locus_tag="PKK85_00705"
     CDS             complement(4432..4791)
                     /locus_tag="PKK85_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24576.1"
                     /translation="MEPEVNFSLICPECGVGNSPQALNCLVCDRDLEKTLAFLEDDSF
                     DLEVTIECLVEYRKNFWGTNRTGKVNQYHRDRMENLEFGEEISRFKFDYEGQRKVLAF
                     RKENLEKLKALIGDSPG"
     gene            4847..4993
                     /locus_tag="PKK85_00710"
     CDS             4847..4993
                     /locus_tag="PKK85_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24577.1"
                     /translation="MKVDKIQGGGSHLHLAQLGIVPADLKANYRGTEGTEFPGLCPRG
                     SDAG"
     gene            complement(5014..6081)
                     /locus_tag="PKK85_00715"
     CDS             complement(5014..6081)
                     /locus_tag="PKK85_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS24578.1"
                     /translation="MNPKFEEISGYSLEDVPHGKEWFKKAYPDPETRHTAISTWIEDF
                     EGSKPGDKASRIFPITSKDGKTKIIYFVPVHLENGEYLMTLEDITKQMEVERALKGSE
                     KKFRTLAQTAMDAIIITDDEGVVIFSNRSLERIFDYSEEDIRGQPVDILIPERYQADF
                     AGQMELPQSKPDNGSGKVFESYGLRKDGSEFPLEMSLNTWQMEGKCYTTVILRDITRR
                     KLNEFKMKMKEDIFQLMARNIEEVFWMVDPLNGQLLYMSPSYQKIWGSSISKLYQNPR
                     SWIDTVHPDDQKKFIHHIYGSLEINDLTRIKSLECRLELPDGTTRWIRIRSFPVINEN
                     KQIYRRVGLAKDITYQKTGRE"
     gene            complement(6078..>6428)
                     /locus_tag="PKK85_00720"
     CDS             complement(6078..>6428)
                     /locus_tag="PKK85_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS24579.1"
                     /translation="LEEVQGAMFPDLKWWTLTPQLWQSIQQDIELCARGETIKEELQI
                     YTKNGPLWIEYSMHPIYDQEGKLQYLVPEGRDISSQKKIEDNLIAEKSKLRTITENAP
                     FGLVLIDAEATIYM"
BASE COUNT         1534 a         1583 c         1589 g         1722 t
ORIGIN      
        1 catatactgg tttgaccggg ccgaggaccc cctaatgaag gtggttcccc atggccgtac
       61 caagaaaaga ggcatatttt ccactcgggc cccgccacgt cccaatccca ttggcctaac
      121 catggtggat ctgattaaac gtgaaggccc ggtgttgact gtgcgggggc tggatgccct
      181 ggacggatcc ccggtgcttg atatcaagcc ctacctgccc gacctggaca gtgtttgatt
      241 ctgatagatt cttattttat acttagactg cgcagacggt tcaaagcagc tgctaattca
      301 aaggggcgaa accctgctaa atccggggga ttttcacgtc ccggtgggct gagaacatcc
      361 aggcccttcc tttccagatc agcttccagc cttgagagta tctcccggag ggtgctgtgg
      421 ccatccatgt acttttgggc atggtacaag gcgtaggcca gggcccgggt ctggctgggg
      481 tccaccaact gtttcacctg ggaaaggtcg atggtgtcac tgcccaggag tatggtgtcc
      541 ttaccccgat tggatatctt catcctccgg cctcggtagg gatttatact ctccttcagt
      601 ggcgtgcggt gccggtagga gaaagggtac ggtacctctc ggctgcggcc agtgggtatt
      661 tgctgggcca ccttccgggc ttccagggtt cggttgtggg gttggtagca atccatcatg
      721 atgatggtgt gggccacctc aaagtaatct ccggatccac ccataaccat gactgtggaa
      781 actccctcct ggtcatataa ctcctgaaca cggtccaagt agggggttat gggttcttgg
      841 ttggatttaa ccagggcctg cattctttca tctctgatca taaaattggt agctgaggtg
      901 tcctcgtcaa agagcagaag ctgagaacct gcttccaggg cttccacaat gttagcggcc
      961 tggctggtgg agcccgaagc attctgggtg gagaattcct tggtgctctt catgaagggc
     1021 ggttcatgga tgaaagagtt aatatcggtt ttctggatgc tgcgaccatc ttcggcacgg
     1081 attttaactg cagattccct ggtgaccacc agttcccggc cgtcccctgg gaggtggttg
     1141 tagacccctg cttccacagc ctggaggagg gtggatttac catggtaacc ccctcccacg
     1201 atgagggtca ctccctctgg gatgcccata ccagttatta agccctggtt aggagaatta
     1261 agggatatcc ttaatgattc cggggatttg aagggtactg ctccacttag gggttgggag
     1321 caaattccgc tacgacgggg tagtgtggag ccattggcta taaaggccac taatccctct
     1381 atatacaatt cctcccggat ggcctgggca tcttcagcca ggtggaggtg ggattccacc
     1441 tccgggttcc cggtgaaaaa acaggaatca tctacaatat ggggtaaaat ctccactaga
     1501 atcttttttg tatcttcccc cattattttc ctgcccttcg ctgataagcc cagtgtaaag
     1561 cgtatttcca ggttttcagg atttatgttc acgcagcttc gctccaggat ttcctggcct
     1621 ccggcatcaa tcctcattac accactccgg ccactgcccc ttggacgggt cagatgggcg
     1681 tggatgctcc tctggaagga ccgtgctata taatcctcca gtgccaccct ccggatggag
     1741 gtgctgaaaa agtgaggtgg atagtggtgg tcctccacct ccacccgtaa atgggaggga
     1801 ctggcgtagg ggtcccgttg cacatggtct acgtggaggg tgaactggtt aaactggtag
     1861 ctaccacgca ggtcctggta ggccttgtag ccccggccat ggattcttcc caggatccta
     1921 gctaagtagt ggtcatcctt cataacccgc acccctagat ttgaaagaat aagaagaaga
     1981 ataaaatgta gttgtattca gatgatggcc agttccccgg cggaggttat ctccagttgc
     2041 tgagagtcta tgaatcccat ctcccgcagc tcccggatat ggttgtaggt catgccccgg
     2101 ctgataccga ttttttgggc cagattcttc accgaggtgt ctcctttttt tagttcctcc
     2161 agtaccatga gtttggtctt ggagatgccg aagttgagga tgggcagatc cacaacttca
     2221 ccgtcttcct cggtgacgta cacgatcctc tccaccattt tgtgacgggc gtagcagccg
     2281 aacagggccc ctagtgcctg gggtttgcgg ccgccgctga tattcaccac caccatcctt
     2341 ccctgggcat tttctgcctc aatgacctca gcggtgtcct gggctatctt caccacctcg
     2401 tagatactgg ttagctgggt cgtgatttcc atgacccgac caactgtttc aatgagcatg
     2461 cgctctgctt ccaacttctt ctctggtgca tcctcctcaa gcaacaaaat gagtttctta
     2521 ggagaaaaac gcgttataca tgccatcaca ggttccaaag agtaaatagt tgatattaaa
     2581 gtgttttcta tattaacaca tcccatcatc ccttcaagaa gggttctaat cttggtttcc
     2641 cctatcaata tccagatgga ataatagctg gacgtttaac aagggaccat ttttggatac
     2701 attcttatat aatattgaat attagtcacc ctcctatata aatttgttta agaatcaacc
     2761 tatttttcgt taaatactta gaaaatattg acatatactg gtttaatccc tagatagtac
     2821 tctgcccctg taaggtgggg gatggcttat cctaaaaaac tatgatcatc ctggtgtaga
     2881 gctgatccaa gcagttacaa catgttttcc aggaggattt tgcagccaat ggcgatgagt
     2941 atgatgccac ccaccagttc catctttccc tcgaagaggt ggcccacttg ttttccgatg
     3001 tacaccccca ccagggagag gaggaaggtc accagaccga tgatgattat gggctgtaag
     3061 agggagatgt ttaaaaaggc aaaggtaact cccacggcaa aggcatcgat actggttgct
     3121 accgacagaa atagtagttc ccggagggat agtagcttac aagcctcctc ctcttctggg
     3181 tggtggaaac cctcgtagat catcttaact cctattaaaa caaggagggt gaaggccacc
     3241 catggcgccc attggttcat gaaactggcc agttcccggc cggaagccca ccccagtacg
     3301 ggcattagcg cttggaatcc tccgaatagg agtgcaatgg taagggcgtg ttttatatcg
     3361 cagcgcacac tcaagccccg ggtgatggag acgctgaagg cgtccatggc caggcccact
     3421 gccagtaaca gaatagccag aagatccata ataaaacatc cttaaactcg ttaagaccat
     3481 tttctataat tctatatcta aggagggtac taatataaac catcaacatc atttgaagtg
     3541 gagctaaaat aaaaaaaatg aggtagtaac atccccaagc aagctaatga agatctgaaa
     3601 gaccacctgt actggtttcg aagaggaaat cagctaatga aggctggttt ttattctgat
     3661 gccgttctgg cctatgaccg ggccctggag cttgatccgg ataattcctt tgtgtgggac
     3721 aaccggggtg tggccctgtc ccatctgggt ttagtggaag agtcccagga atccttccaa
     3781 ctagccctgg actacgatcc atccaatgca caggcctggt ccaacctggg cgttgccctg
     3841 gcatcccagt accgcctgga ggaggccctt aactccctgg atcaggcggt gaagctggat
     3901 gaatccaatg atgaagcctg gaacaacagg ggtaccgccc tcttggctct gaaaaaatac
     3961 cgggaagcag tggaatcctt tgataaggcc ctagaaatta attctaagaa tcctcaagcc
     4021 tgggctggta agggatcctc cctgggtttc ctgaaacagt accaggaagc cattgagtgc
     4081 atggagcgat tcatagatct ggcctctgct ctggacagcc cccaagtaga ggaagcttgg
     4141 gccatgatcc tggagtggaa gataagaatc aagggataga agtgaaatta taatatttaa
     4201 taattattgg ctgttgatta ataagagagc ttccagagat gagggggtgt gtaggagtga
     4261 aaaaatggcc tatcgttttt atctctggaa gctatggttt ctggtttcaa cgagaaatag
     4321 tagtttcaag tgttgaaacc aaactaggta tgttgtgata taagtacaca ctgccatata
     4381 tactttgcgg tttggatgct tctgatctgg acccccaacc aaagagtccc ctcagccagg
     4441 gctatcacca atcagtgcct tcagtttctc caagttctct ttcctaaagg ctaaaacctt
     4501 tctctgcccc tcgtagtcaa atttgaagcg ggatatttct tctccaaact ccaggttttc
     4561 catcctatcc cggtggtact ggttcacctt accagtacgg ttggttcccc agaaattttt
     4621 ccgatactcc accaggcact cgatggtcac ctccaggtca aaggaatcat cctccaagaa
     4681 ggccaaggtc ttctccaggt cacggtcaca caccaggcag ttcagggctt ggggggaatt
     4741 gcccacccca cactctggac atatcagtga aaaattaact tcaggttcca tgctaccatg
     4801 caataatgtg gatcagggca cttatataca taatttgtgg ggagaagtga aggttgataa
     4861 gatccaaggt ggtgggtccc atcttcacct ggcgcagctg ggaatagtac ctgcagatct
     4921 taaagctaac tatagaggaa ctgaaggcac tgagtttcct ggactgtgtc ccaggggatc
     4981 agatgctggt taataataaa aggaatgaat tctctattct ctaccggtct tctggtaggt
     5041 tatgtccttg gcaaggccta ccctacggta gatctgcttg ttttcattta taacagggaa
     5101 ggaacggatc ctgatccaac gggtggttcc atctggaagt tctaagcggc attccaatga
     5161 ttttatccgg gtcaagtcat tgatctccag tgaaccgtag atgtggtgga tgaacttctt
     5221 ctgatcatct gggtggaccg tgtctatcca ggaccgggga ttctggtaga gtttacttat
     5281 ggaggagccc cagatcttct ggtaagaggg actcatgtag agtaactgtc catttaatgg
     5341 atccaccatc cagaaaactt cctcaatatt ccgggccatt aactggaaga tgtcctcctt
     5401 catcttcatc ttaaattcat tgagcttgcg gcgtgtgatg tcccttaaga tgactgtggt
     5461 gtaacatttt ccctccatct gccaggtgtt aagggacatc tccaggggga attcactgcc
     5521 atccttacgc aaaccataag actcgaaaac tttaccggaa ccattatccg gcttggattg
     5581 gggtagttcc atttgcccgg cgaagtcggc ctggtacctc tccgggatca ggatatccac
     5641 tggctgtccc cggatatcct cttcactgta gtcgaagatc cgttccaggc tgcggttgga
     5701 aaaaataacc accccctcat catcggtgat gatgatggca tccatggctg tctgggccag
     5761 ggtacggaat tttttttcac tacccttcaa ggcccgttcc acctccatct gcttggtgat
     5821 atcctccagg gtcatgaggt actcgccgtt ttctaaatgg actggtacga agtagatgat
     5881 cttggtcttt ccatccttac tggtaatggg gaaaatgcgg gatgccttat ctcctggttt
     5941 agatccttcg aagtcttcaa tccaggtaga aattgccgta tgccgggttt caggatcagg
     6001 gtacgccttc ttaaaccact cttttccatg gggcacatct tccaagctgt aacctgatat
     6061 ttcctcaaat ttgggattca catatagata gttgcctcag catcaatcaa caccagccca
     6121 aatggggcgt tttcagttat ggttcgtaat tttgattttt cagctattaa attatcctca
     6181 attttcttct gggaactgat atccctacct tcagggacca ggtactgtaa cttaccctcc
     6241 tggtcgtaga tggggtgcat gctgtactcg atccaaaggg ggccgttttt agtgtagatc
     6301 tgcagttctt ccttgatggt ttcaccccta gcacagagct caatatcctg ctgtatactc
     6361 tgccagagtt ggggggtgag ggtccaccat ttaaggtccg gaaacatggc accctgcacc
     6421 tcctccag
//
LOCUS       DAOQVW010000018         6416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_13323_length_6416_cov_3.451973, whole genome shotgun sequence.
ACCESSION   DAOQVW010000018 DAOQVW010000000
VERSION     DAOQVW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13323_length_6416_cov_3.451973"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKK85_00725"
     CDS             <1..302
                     /locus_tag="PKK85_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNS24580.1"
                     /translation="APGMVSPLRAFTEFTLAPSGLAYYQGSLYSAGLRGNQLRQIKLS
                     PDGRSVVGEEVLFQDLGRIRDVVEYNGYLYISTSNRDGRGIPKSGDDRIIRVMVS"
     gene            complement(317..850)
                     /locus_tag="PKK85_00730"
     CDS             complement(317..850)
                     /locus_tag="PKK85_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HNS24581.1"
                     /translation="MDVKVLRLNHRRIRDARITTHVCLTARAFGASGLFLDGDHDQKL
                     IKNVQDVVKRWGGSFQMDYEKNWSNLLDEWKKSGGEIVHLTMYGEPVQKVTSTIKKSK
                     KNKLVVVGGSRVPTKVYEEADWNVAVTGQPHSEVSALAIFLHLLFEGKELDLEFEGGE
                     IKVLPLAKGKKVIIDQG"
     gene            complement(856..1671)
                     /gene="cobS"
                     /locus_tag="PKK85_00735"
     CDS             complement(856..1671)
                     /gene="cobS"
                     /locus_tag="PKK85_00735"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNS24582.1"
                     /translation="MSSKARLDAPVRKGEMISKGNRTPVWRGFLGLVSFSTILPLNIH
                     TSILEMARFTWMWPLIGGFIGIMVGAVAWVVTGALHLPQLLSASLIYAFSIIITGLHH
                     LYGLIDFGDGIMGHGDTERRIEIMKDQRIGTGGLSSLLITSLITLTALSVFPIALLIP
                     IVIVAEIAAKLGLVTCATFSRPSGKGTGTYFVENMTPGLLGISLTICILLGYSAMQYA
                     GVAGIAGGVIMGIFMAFLARWKFKYTTGDVLGATNELSRMSALLAMVLILTMW"
     gene            complement(1673..2722)
                     /locus_tag="PKK85_00740"
     CDS             complement(1673..2722)
                     /locus_tag="PKK85_00740"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNS24583.1"
                     /translation="MEVTIGVHDFDLFMDQNSIILEGKDEFILFGCSKGYSGCGKFKS
                     VSNHTLKPDVGRVVEDENRYIEDLILEKKMATPAAVVLTPFASIVNEIVTIGPVSAII
                     SMPKEATTPPIYGNIFISTLVVVDQALDQGILLKLLMEIQETLANVLWDFGLRKGIST
                     KNSPHPILIVCKRLGGGENPDKTPELEDLIRKCVEKASSSLLGDLGYPLSITQILSAS
                     GVEIDDLTAAGAELLVGVDDSPEIRSRIKTELEKALGDVNVVSMVLAGIRVDEDYQHH
                     RVRGVDVDDDPAYLYADEVLGMAIANQIAGTKAIFNFKRYDEKKPGIISKLGPMLDDV
                     CAGLVAGCMSKIFEE"
     gene            2785..3300
                     /locus_tag="PKK85_00745"
     CDS             2785..3300
                     /locus_tag="PKK85_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MJ1244 family protein"
                     /protein_id="HNS24584.1"
                     /translation="MKAHLRVFVEVENLGKAINALTDAGITGFYVLEYKGLSPQDWKG
                     FSIKEDPKSAISLIRDMATDAVLVCSVVEEKKVDGIVDALTESLKGEKYTILEVPIRR
                     IIVNYGDQEKAERTETWLLEKDVQCFYCGEKAIQKIKIDTNRAKIWCTNCGTTRHYVI
                     KGSEKGWDDEH"
     gene            3290..3463
                     /locus_tag="PKK85_00750"
     CDS             3290..3463
                     /locus_tag="PKK85_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24585.1"
                     /translation="MSIEKWNLKKSANCFKCGDATIHDVEVDTYTLKISCRDCGFTRY
                     YKFHMVETPAQRD"
     gene            3573..4793
                     /gene="larC"
                     /locus_tag="PKK85_00755"
     CDS             3573..4793
                     /gene="larC"
                     /locus_tag="PKK85_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNS24586.1"
                     /translation="MVVIIDPQTAGIAGNMVLGALIDLGADIEGTKGVMEHFTNPFGH
                     VKVELGKINKNGLCATLADVNAFDRDSICYNELVKILKDMGRGGMSIEAFDLAMKVFE
                     TLARAEARIHGVDLKDVHFHEVGAADAVADVMGSVYAFHDLELHTHTVYGLPVALGGG
                     VKETKHGLLSIPAPATLEILKNTPTLGGPVTEELSTPTGAALYVNMVDEYCQFQPLLT
                     HKFTGYGAGKMDLDFPNVLRIIKGDSAIPSDNVVLLETNMDNVSGEILGNLFNRLMAE
                     GALDVTLIPVLMKKNRPGHLLRVIARQQDCDTISEVIIRETGTLGVRVMPFVHRNILE
                     REIIPIEIEISGTPAKIRIKVGKIGNEIINTAPEYEDARQIADKMQIPIKEVMRMADE
                     AFWHQISLGDITVG"
     gene            4839..>5111
                     /locus_tag="PKK85_00760"
     CDS             4839..>5111
                     /locus_tag="PKK85_00760"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNS24587.1"
                     /translation="MNSKPKATSVLSGGLDSTVATFYFKDQYQIHALTFNYGQRSAEM
                     EIKSSQAICDVMGIEHTVIDLPWLATLGNSALTSQEEVPRLGMDQLD"
     assembly_gap    5112..5121
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5122..5517
                     /locus_tag="PKK85_00765"
     CDS             <5122..5517
                     /locus_tag="PKK85_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNS24588.1"
                     /translation="QTARKVWVPGRNIVFTAIATSFAEAEGASAIIVGWDQEEAVTFP
                     DNSLEFLEAFNQVLEVGSLDEIEIKAPLINLNKTEIVKLGDRVDAPLHLSYSCYMGGE
                     GHCGVCESCMRRKRAFYNSGVVDRTLYGD"
     gene            5967..>6416
                     /locus_tag="PKK85_00770"
     CDS             5967..>6416
                     /locus_tag="PKK85_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24589.1"
                     /translation="MKGTKHLHNVPIFSKQIGVIAFLFLIVFSSGCISNDEGSNNFTV
                     VNKTGNNSYSVDGVSFRCPDNWVVATDNVNGNITIMASQIDEFNDSTSNSFGPVKAGY
                     SAFSIDNPQFEVHITSNNGISDQEAIDQVKNQISALGNKISSEKWVVN"
BASE COUNT         1829 a         1334 c         1429 g         1814 t
ORIGIN      
        1 ctgccccggg tatggtttca cctttgcgtg ctttcacaga attcacactg gctccttctg
       61 gactggccta ctaccaggga agtctatact ccgcgggttt aaggggcaac cagcttagac
      121 agataaaatt atcgccagac ggacgatcag tggtgggaga agaggtcctg ttccaggatc
      181 tgggccggat tagggatgtg gtggagtata atgggtacct ttacatctcc acttccaatc
      241 gagatgggcg ggggattccc aaatctggag atgacaggat catacgggta atggtaagtt
      301 aatttaaggc tactttttaa ccctggtcaa taatcacttt tttacccttg gctaatggta
      361 aaaccttgat ttctcctccc tcaaattcga ggtcaagttc tttaccctca aatagcaggt
      421 gcagaaatat ggccagggct gatacttctg aatggggttg accagtcacg gccacgttcc
      481 agtcggcctc ttcgtaaacc ttggtgggga cccgggatcc acctaccacc accagtttgt
      541 tctttttaga ttttttgatg gtggaagtta ccttctgtac gggttccccg tacatggtaa
      601 gatgcacgat ctcgccacca ctctttttcc attcatccag caaattagac cagttttttt
      661 cgtaatccat ctggaaagaa ccaccccagc gtttcacaac gtcctgcaca ttttttatga
      721 gtttttggtc atgatctccg tccaggaata gtccagacgc tccaaaagcc cgggctgtta
      781 aacagacatg ggtggtaatt ctggcgtcgc gaattcgccg gtggttcagt cttaaaacct
      841 ttacatccac ttttatcacc acatggtcag gattaaaacc atagctagaa gggctgacat
      901 tcgggataat tcattggttg cccccagcac atcgccagtt gtgtatttga acttccaacg
      961 ggctagaaag gccatgaaaa ttcccataat aactccgcct gctattccgg ccactccggc
     1021 atactgcatt gcagagtatc ccaggaggat gcatatggtc agggaaatgc ccagcaatcc
     1081 aggagtcata ttctcaacga aataggtacc agtaccctta cctgagggcc tggaaaaggt
     1141 ggcacaagtt acaagcccta atttagctgc aatttcagct acaatgacta tgggtattaa
     1201 taaagctatt ggaaagacgg atagtgcagt taacgttatg agggatgtta ttaaaaggga
     1261 ggaaagtcct ccagttccaa tccgctggtc tttcatgatt tctatccttc tttcggtatc
     1321 accatgaccc ataatcccgt ctccaaagtc tataaggcca tataggtgat gtagcccggt
     1381 gataataatg ctgaatgcgt atatcaggga tgcagataag agttggggta ggtgaagtgc
     1441 acctgtcact acccaggcca cagcacccac cattattcca atgaaacccc caattaacgg
     1501 ccacatccag gtgaaccttg ccatttccag gatgctggta tgaatattta gggggagtat
     1561 ggtggagaag gagacaagtc ctaggaaccc cctccaaacc ggggtcctgt tacctttgct
     1621 gatcatttca ccctttctga cgggtgcgtc taatctggct ttacttgaca ttttattcct
     1681 caaatatttt ggacatgcag ccagccacca atcccgcgca cacatcatcc agcatggggc
     1741 ctagtttgct aattatgcct ggcttttttt catcatatct tttaaaatta aatatagctt
     1801 tggtaccggc tatttgatta gcaatggcca ttccaagcac ttcatcagca tataagtagg
     1861 ctggatcatc atccacatcc acgcccctca cccgatgatg ctggtaatcc tcatctaccc
     1921 tgataccagc cagaaccatg gaaaccacat ttacatcccc taacgctttt tctaactctg
     1981 ttttgatcct ggatctaatc tctgggctgt catctacccc taccaacaat tctgccccgg
     2041 cagcagtcag gtcatcaatt tccactcccg atgcacttaa aatctgggtt atggacaggg
     2101 ggtaccctag atctcccagg agggatgagc tagctttttc tacacatttt cgaattaaat
     2161 cttccaattc aggggtttta tctggatttt cccctccacc caatctttta catacgatta
     2221 gaataggatg ggggctgttt ttagtagaaa ttccctttct aagaccaaaa tcccataaaa
     2281 catttgccaa agtttcttgt atctccatta gcagttttag tagaatgccc tgatccaggg
     2341 cttggtctac aactaccagg gtggaaataa atatattccc gtagatggga ggtgtggttg
     2401 cttccttagg catgctgata atggccgaaa ctgggccaat tgttacaatt tcatttacaa
     2461 tactggcaaa tggggttaag acaaccgctg caggagtggc catttttttt tctaaaatca
     2521 gatcttcaat gtacctgttc tcatcctcca ccacacgccc tacatctggt tttagggtat
     2581 gattagaaac agatttgaac tttccacagc cactatatcc tttcgaacat ccgaatagaa
     2641 taaattcgtc ttttccctcc agaattatac tattttggtc cataaacaga tcaaaatcat
     2701 ggacacctat cgttacttcc atcaaaccac ctttcacaat agcctataat agatatgaag
     2761 gatataatta atataggtgg tcttatgaaa gcacatttaa gggtctttgt ggaggttgaa
     2821 aacctgggaa aggccattaa cgcattgact gatgcgggta taacgggatt ttatgttttg
     2881 gagtataaag gattgtcgcc acaggattgg aagggttttt ccataaaaga agaccccaag
     2941 tcagccatta gtctcatacg ggacatggcc actgatgcag tcctggtgtg tagtgtggtg
     3001 gaagaaaaga aggttgatgg aattgtggat gccttaactg aaagtttaaa aggagaaaaa
     3061 tataccatac tggaagttcc catccgccgc atcatcgtca actatggaga tcaggaaaaa
     3121 gcagaaagga cagaaacttg gttactggaa aaagatgttc agtgcttcta ttgtggtgaa
     3181 aaagccatac aaaagattaa aatagatact aaccgggcta aaatatggtg tactaactgc
     3241 ggaacaactc gccactacgt aattaaaggt tcagaaaagg ggtgggacga tgagcattga
     3301 aaagtggaat ctcaaaaaat ctgccaattg ttttaaatgt ggagatgcaa caatccatga
     3361 tgttgaagtg gacacttata cccttaaaat atcctgcagg gattgtggat tcacccgtta
     3421 ttataaattc cacatggtag agactcctgc acagcgtgat tagaactggc ggtattgaaa
     3481 cctttatttt tttatatttt ttcatttaca gaatcataga aatgatccaa aaaatctatc
     3541 taaattttat aatagtacta gaagtgatgt ttatggtagt gatcatcgac ccccaaactg
     3601 caggaatagc tggaaacatg gttttagggg cattaatcga cttaggggct gatattgaag
     3661 gcacaaaagg tgtcatggaa catttcacta atccctttgg acatgtcaaa gtagaactgg
     3721 ggaaaataaa taaaaacggg ttgtgtgcta ccctggctga tgttaacgcc tttgacaggg
     3781 attcaatttg ttacaatgaa ctggtgaaaa ttttaaaaga tatggggcgg gggggaatgt
     3841 caatagaggc atttgatctt gccatgaagg tatttgaaac cctggcccgt gctgaagccc
     3901 gaatacatgg tgtggattta aaagatgtcc atttccatga ggtaggtgcg gctgatgcag
     3961 tggccgatgt aatgggttcg gtctacgcct ttcacgactt ggaattacac acccacacgg
     4021 tttatggatt gccagtggcc ctgggtggag gtgttaaaga aaccaaacac ggcctcttaa
     4081 gcatacccgc cccagctaca ctggagatct taaaaaacac accaaccctg ggggggccag
     4141 taactgagga gctcagcacc cccactggtg cagcccttta cgtcaatatg gtggatgaat
     4201 actgccagtt ccagcccctg ttaacccaca agttcacagg ctatggggcg ggtaaaatgg
     4261 atctggattt tcccaatgtt ctgaggataa tcaagggtga ttcagcgatt cccagcgaca
     4321 atgtggtact attggagact aacatggaca atgtctctgg ggagatactg ggcaacctct
     4381 tcaataggtt aatggctgaa ggggcgttgg atgtcacctt gataccggtg ttaatgaaaa
     4441 aaaaccgtcc tggccatctc ttgagggtta ttgcacgtca acaagactgc gacaccatct
     4501 cagaagtaat cataagggaa actggtacat taggtgtccg ggtcatgccc tttgtccata
     4561 ggaacatcct ggaaagggag atcatcccca tcgaaataga aatatctgga accccggcga
     4621 aaattcgcat aaaagtgggg aaaattggta atgaaataat taacaccgcc cccgaatatg
     4681 aggacgcacg tcaaatagca gataagatgc aaatccccat taaagaggtg atgcggatgg
     4741 cggatgaggc attctggcat cagatctccc tgggagatat taccgtaggt taattttttg
     4801 agaaaactga gtaaatatat taatttggag ttaacaatat gaattcaaaa cccaaagcca
     4861 catccgtact ctctggaggt ttggattcca cagtagccac cttttacttt aaggatcaat
     4921 accagataca tgctctgacc ttcaattatg gtcagagaag tgctgaaatg gagataaagt
     4981 cctcccaggc catatgtgat gttatgggca tcgagcacac ggtgattgac cttccctggt
     5041 tagctacact gggaaactcg gccctcacca gccaggaaga ggtcccccgg ctggggatgg
     5101 atcaattgga tnnnnnnnnn ncagaccgcc cgtaaagtgt gggtgcccgg acgtaacatt
     5161 gtatttacgg ctattgccac ttcatttgcc gaggcagaag gagctagtgc catcattgtg
     5221 ggttgggacc aggaggaagc agttaccttc ccagataact ccctagagtt cctggaagcc
     5281 ttcaaccaag tcctggaagt agggtccctg gatgagatag agataaaggc cccacttata
     5341 aatctgaaca aaaccgagat agtaaaatta ggagataggg ttgatgctcc tttacatttg
     5401 agttactcgt gttacatggg tggggagggg cattgtgggg tttgtgaatc atgtatgagg
     5461 cggaagaggg ctttttataa ttctggtgtt gtagatcgga ctctttatgg ggattaagcg
     5521 tgatttagca aggtgcatct ctttatgagg tcttgtgggg agtttatttt aaatttggta
     5581 taaactaata tcttttttta ttcgctattc ttttaaatct aagcagtata tctgctactt
     5641 cttgagttta cagatgaaat ttgttttttt agaacaaaat ctttttgttt gaagatattt
     5701 taacaatatt tatactattt ttgggtgggt tcgctgctac ttacctttcc agaactaata
     5761 aagccagaat aggttattat aacaatatgc aataggttcc ttactgggac ttatttttat
     5821 ctttaaaaca ggattaacaa tttattccct attaattttg tttttagtat tcccaatctt
     5881 cggacttctt ggaggattca tcgctaaaaa actaagaata cgcttagata aacaatctac
     5941 agcataacta atccggtgat ctggacatga aaggaaccaa acacttacac aacgtgccga
     6001 tattcagcaa acagattgga gtgatagctt tcttattttt aattgttttt agttcaggat
     6061 gtataagcaa cgatgaaggt tcaaataatt tcacggtagt caataaaact ggtaataact
     6121 cttattctgt ggatggggtt agttttaggt gtccggataa ttgggtggtg gctactgaca
     6181 atgtaaacgg aaacattacg attatggctt ctcagattga cgaatttaat gattcaacat
     6241 ctaattcatt tggtccagtt aaagctggtt acagtgcttt ttcaattgat aatccccaat
     6301 ttgaggtgca tataacgtcc aataatggca tatcggatca ggaagcaatt gatcaggtca
     6361 agaatcaaat atctgccctc ggaaataaaa tttcaagcga aaaatgggtg gtcaat
//
LOCUS       DAOQVW010000019         6374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_13440_length_6374_cov_3.166957, whole genome shotgun sequence.
ACCESSION   DAOQVW010000019 DAOQVW010000000
VERSION     DAOQVW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13440_length_6374_cov_3.166957"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1545)
                     /locus_tag="PKK85_00775"
     CDS             complement(85..1545)
                     /locus_tag="PKK85_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS24590.1"
                     /translation="MSVIIIGAGIAGLAAGCYLQMNGFESRIFEMHNKPGGLCTAWKR
                     KDYTFDLCISWLTGSSPQSPVYSLWEELGVVQDREFITIEYGKHVIDEQGNKFIVYAD
                     PDKLRDHMLSFSIEDEKLIKRFTSDLKKLGTIRLQIEMGIIDLIKMIPLIRMFRKYSI
                     TATEMASKFKNPVLRNLFQIAFNWYDQSMIVLMLGIAQRGSKLSGYPIGGSLPVARAI
                     ERRYMDLGGDIFYNTEVRKIIVENNKAVGVQFIDGSEYKGDIIVSGADGHSTIFQWLD
                     GQYIDDKIQGFYENLDLFPPLVFVSLGVNDDYSNEPHYLSFPLKNPITVGGGKIDRIL
                     LRNHSFDPNLAPEGKTVFTVMIEANYDYWAKLKGKKEEYLAEKKNIEESMINAISEIY
                     PGIEDKIEVIDVATPLTFVRYTGNWRGSHEGWLWNQKVSFTLEIPQTLPGLSHFYMAG
                     QWVSPGGGLFGVATTARKAVKMICKNEKQRFVTTKP"
     gene            complement(1592..2086)
                     /locus_tag="PKK85_00780"
     CDS             complement(1592..2086)
                     /locus_tag="PKK85_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24591.1"
                     /translation="MDIEDIPAEVRWKIATKASNMASVAQMNAFEEVAGKEAANQMED
                     IIMGEGGKQAGELAIQLGLPANNAIETSEAWGIVGTIMLGPEIKSEVVEADEDHIVDR
                     ITGCAMLNAHQELGVPLTGLAEGCQAYNTNAVEAINPKYTQHFAKRMCAGDEFCESHV
                     ELKK"
     gene            complement(2131..2784)
                     /locus_tag="PKK85_00785"
     CDS             complement(2131..2784)
                     /locus_tag="PKK85_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS24592.1"
                     /translation="MTPRMQRKKKKKKKAILDAAERIVADRGMAGMTMGEVADAADVA
                     TGTLYLYFKNKGSLLAAVNARLNHQVNIYIKEKMDLKKTGLEKVVAMGQATVEFFMEN
                     PQKWKAVTELYQMKVQDPHDPNVQDFLEVTNEMVQMMAQAYQQGIEEGSIRQDLDPVA
                     TAIYNRMAFGNAFTPTTEQKMLLEHNKITPERYLSVAWGLISHSTRGVLLEKKVKKS"
     gene            3047..3589
                     /locus_tag="PKK85_00790"
     CDS             3047..3589
                     /locus_tag="PKK85_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24593.1"
                     /translation="MEKISLMVLVLWVAIGVSLSTLYIPVLSDSQEVLINTLNSATDH
                     IIGIDMNFSLPLVSLVQGEFHKDYFEIYSNSGATLNNTGVPLTNTENKFKIPTSNLPT
                     TVVPLKPSPTTPSKEPTNTPPTTGITDKHTSTQDTEKNSSIKKPNHNIPKYNLNYHKK
                     YYRNHHQHQHPQKTSRGCKK"
     gene            3831..>4361
                     /locus_tag="PKK85_00795"
     CDS             3831..>4361
                     /locus_tag="PKK85_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24594.1"
                     /translation="MRKITLFIFIIWIAIGMSVAALYIPVSSDPVEAQRPIFEQENLL
                     CPQNDISPLTSPINSTLINRTNIDLSLDVLSPIVALVQVQLNSLTGNGPFVSLIQVKL
                     EPTVIITDKVVTTDKPLETKTTVELPKDEVKTKSMVTNTINTTVIGTKTTVIETNTTE
                     TNTTETNTTETNTTETN"
     assembly_gap    4362..4461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4462..4794
                     /locus_tag="PKK85_00800"
     CDS             <4462..4794
                     /locus_tag="PKK85_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24595.1"
                     /translation="TTDLGAGSKYLVENLSFINTQFMVEITNQNNNVISGGFYEISTD
                     PSSNHANVLQSGTFPYAPGTFTISNVLTFTPADNIRYYILVYTNYPESDNRVSKDNAI
                     FTYSDMVS"
     gene            4858..5199
                     /locus_tag="PKK85_00805"
     CDS             4858..5199
                     /locus_tag="PKK85_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24596.1"
                     /translation="MKKITLFVIIIWIAIGMCIAVIALPSISSDSGKIPAIAQDTVKK
                     SNGKNSVDESTSNSTNKTKLTNQDQKKNNNSVYPRSDQLMKKHSFKSMVKHDKKMIKT
                     KHSSKKHDQKA"
     gene            5389..5580
                     /locus_tag="PKK85_00810"
     CDS             5389..5580
                     /locus_tag="PKK85_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24597.1"
                     /translation="MMVSPMTGAVMVGSATVSGQILALRHTGRWFRNSYPINIFPVAD
                     GGGWINTRNCGTGRRCQKK"
     gene            complement(5750..5986)
                     /locus_tag="PKK85_00815"
     CDS             complement(5750..5986)
                     /locus_tag="PKK85_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24598.1"
                     /translation="MGIGRGGVNVTDLDLIVYLSMFQSGAQITSDLNLEELYQLQLRP
                     IEDTNKRSVDFDGTKFSQMIVAAIMSSPTPKLLG"
BASE COUNT         1843 a         1446 c         1094 g         1891 t
ORIGIN      
        1 accttgtgcc gccatgttca accattactt tggttatggg ctgtattggg gaagatgtgt
       61 agaaatgggg tccaatacaa tggtttaagg ttttgtggtt acaaaacgct gcttttcatt
      121 tttacatatc attttaacag ctttacgagc agtggttgcc actccaaata atcctcctcc
      181 tggagaaacc cactgtccag ccatgtagaa gtgagataat ccggggagtg tttgaggtat
      241 ttccaaagtg aaagacactt tttgattcca taaccaacct tcatgtgatc ctctccagtt
      301 tccggtgtaa cgtacgaaag ttaggggtgt ggccacatct atcacttcaa tcttatcttc
      361 gatacccgga taaatttctg aaatggcatt aatcatagat tcttcaatat ttttcttttc
      421 agcaagatat tcctcttttt taccctttaa tttagcccaa taatcgtaat tagcttctat
      481 cataactgta aatacagttt ttccttctgg agccaaattg ggatcaaaac tatgattcct
      541 aagcagtata cgatctattt tcccaccgcc cactgtaatg ggatttttta atggaaatga
      601 tagataatgc ggctcgttac tgtaatcatc atttactcct aaagaaacaa agaccagagg
      661 aggaaataag tccagatttt cgtaaaaacc ttgtatttta tcatctatgt actggccatc
      721 taaccattga aatatagtgc tatgaccatc tgctccagat actattatat ctcctttata
      781 ttcagaaccg tctataaact gcactcctac cgctttatta ttctctacaa taattttcct
      841 gacttcagtg ttataaaaaa tatctccacc aagatccatg taacgacgtt caatggcacg
      901 ggccactggt aaagaacccc caattggata ccctgataat ttacttcccc gctgcgcaat
      961 acccagcatg agcactatca tggattgatc ataccaatta aacgcgattt gaaatagatt
     1021 ccttaaaaca ggatttttga atttagaggc catctctgta gcagttatag aatattttct
     1081 aaacattcta atcaaaggta tcattttaat taagtctata atgcccattt caatctgtaa
     1141 acgtatagta cctaattttt taaggtcact ggtaaatctt ttaattagtt tctcatcttc
     1201 tatagagaaa ctgagcatat gatcccggag tttgtcagga tcagcgtaaa caataaattt
     1261 atttccttgc tcatcgatca cgtgttttcc atattctatg gttataaatt cacgatcctg
     1321 gaccacacct aattcttccc agagactata aacaggactt tgaggagaac ttcctgttaa
     1381 ccagctaatg caaagatcga atgtataatc ctttcttttc caggccgtgc acaaaccacc
     1441 gggtttattg tgcatctcga atattctact ttcgaaccca ttcatctgca ggtaacaacc
     1501 agctgctagt cctgctatac ctgctccgat gataattacg gacatagaca ttaaattccc
     1561 cccagtctaa aaaaaatggt tgaattgata tctatttttt caattcaacg tggctttcac
     1621 agaattcatc cccagcgcac atccttttgg cgaagtgctg ggtgtatttg gggtttatgg
     1681 cctctacggc gttggtgttg taggcttgac atccttcagc gagtccagtt aagggtaccc
     1741 ctaactcctg gtgggcgttg agcatggcac aaccggtgat tctatccaca atgtgatctt
     1801 catcggcttc caccacttca cttttgatct ccgggcccag catgatggtg cctacaattc
     1861 cccaggcttc actggtttca atagcattat tagcaggtaa acctaattga attgcaagtt
     1921 caccggcctg tttcccccct tcacccatta ttatatcctc catctgattg gccgcttctt
     1981 tacctgcaac ttcttcgaaa gcattcattt gggccacaga cgccatgtta gaagccttgg
     2041 tggctatttt ccacctgact tcagcaggta tatcctctat atccatatat atcacctctt
     2101 ctttagttaa gatatttatg aggcgtatta tcatgatttt ttaactttct tttctaggag
     2161 gactccccga gtagagtgag atatcaaacc ccacgccaca gacagatacc gttccggtgt
     2221 gattttatta tgttcaagga gcatcttctg ctcggtggtt ggggtgaagg cgttgccaaa
     2281 tgccattcgg ttgtaaatgg ccgtggcaac agggtccaga tcctggcgga tgcttccctc
     2341 ctcgataccc tgctggtagg cctgggccat catctgcacc atctcattgg tcacctctaa
     2401 aaagtcctgg acattaggat catggggatc ttggactttc atctgataaa gttcagttac
     2461 tgccttccac ttctgggggt tttccataaa gaattctacc gtagcttgtc ccatggccac
     2521 tactttctcc agacccgtct ttttaaggtc cattttttct ttaatataaa tattgacttg
     2581 atggttgagc cgggcattga ctgctgccaa caggctgccc ttgtttttaa agtaaaggta
     2641 cagggtccca gtagccacat cagcggcgtc agccacttcc cccatggtca tgccagccat
     2701 ccccctatct gcgactatcc tttcagcagc gtccagtatg gctttcttct tctttttctt
     2761 tttgcgttgc atcctgggag tcatggtgtc tcagattatt tttggcccta ccttcttcta
     2821 actgaatata attcactaat gaatattatt cattaattac tatatatata attttctagg
     2881 atcctatttt ttcaaaagct agaactccaa atgtataagg acatttccca ttatcatccc
     2941 aaacaatata cgttgatgat aaagacaatt agaggagtta atagtagtaa gttatttatt
     3001 tatagagatt aaaataaaat ttagtgctat ttaggggaga attaatttgg aaaagattag
     3061 cttaatggtt ctggtgttat gggttgccat tggagtaagt ttaagcaccc tctacatacc
     3121 cgttctatct gattctcagg aagttctcat taacaccctg aattcagcaa ctgaccatat
     3181 tataggaatc gatatgaatt tttccttacc gctagtttct ttagtccaag gcgaatttca
     3241 taaagactac tttgaaatct actccaactc aggtgcaaca ttgaacaata ctggcgtacc
     3301 attaaccaat actgagaata agtttaaaat tccaacttcg aatcttccga ccaccgtagt
     3361 tcccctcaaa ccttctccta caacaccttc caaagaaccc actaacaccc caccaaccac
     3421 cggcattaca gataagcaca ccagcacaca agacacggaa aaaaactctt ccatcaaaaa
     3481 acccaaccat aatattccta aatacaacct taattatcat aaaaaatact atcgaaacca
     3541 ccaccagcac caacaccccc agaaaacttc ccggggttgc aaaaaatgat gctgggaata
     3601 atatctgatc tgcaccaaga atccctggga ttttaacttt gtgtctcatg ttgatatgga
     3661 atacatgaat tttaagacga atgatttgga aataccaaca actacttgat gttcattcat
     3721 taaagttatt tttaacaggt ttaaagccaa atcaaaattt tttaaacaat tgaatcatca
     3781 atggacaaat ttattaatta ttaactagat aatgatatat cggtgatctg atgaggaaga
     3841 ttacactatt tatattcata atctggattg ctattggtat gagcgtagct gctctttaca
     3901 taccagtctc atcagatcca gttgaggctc aaagacctat ttttgaacaa gaaaatctcc
     3961 tatgcccaca aaatgatatt tcaccattaa ccagccctat caattcaaca ttgattaata
     4021 gaaccaacat tgatctatcc ttagatgtgt tgtctccaat agttgcttta gtacaagtgc
     4081 aactgaactc acttactggt aatggaccat ttgtttcatt aatccaagtg aaactagaac
     4141 caacagtgat catcactgat aaggtagtga ctacagataa accacttgaa actaagacca
     4201 cagtagaact acctaaagac gaagttaaaa ccaaaagtat ggttaccaac accattaaca
     4261 ccaccgtaat aggcacgaag accactgtaa tagaaaccaa cactacagaa accaacacta
     4321 cagaaaccaa cactacagaa accaacacta cagaaaccaa cnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nactacagac cttggtgctg gtagtaaata tttggttgaa
     4501 aacttgagtt tcatcaacac tcagtttatg gtggagataa ccaatcagaa taacaatgta
     4561 attagcggtg gattctatga aatcagcact gacccatcat caaaccatgc taatgttctg
     4621 cagtccggca ccttccccta cgcccctggc acatttacca tcagcaatgt tttgaccttt
     4681 acaccagcag ataacatccg atactacatc ctggtctaca ccaactaccc tgaaagtgac
     4741 aaccgggtgt ccaaggataa cgcgatcttc acttattctg atatggtatc ataatgtaat
     4801 tcattctact ttaacaggca atttatctaa atagggtagt atcactgggg ggattatttg
     4861 aagaagatca ccttatttgt aataattatc tggatagcca ttggcatgtg tatagccgtc
     4921 attgcccttc catccatatc ctctgattct ggtaaaatcc ctgctattgc ccaggatact
     4981 gtgaaaaaat ccaatgggaa aaactcagtg gatgaatcaa cttctaattc taccaacaag
     5041 accaagctaa caaatcagga ccaaaagaaa aacaataatt cagtttatcc ccgttctgac
     5101 cagttaatga agaaacattc ctttaaatcc atggtaaaac atgataaaaa aatgataaaa
     5161 actaaacaca gttccaaaaa gcatgaccaa aaagcttagt ctttttattt attccaaaac
     5221 ttcttaactt cctgggttat ttcctgtccc tggcggacaa gatctcctct tttttctaag
     5281 tccataagac tgagcccgtt aacctcagcg cggcctcatc tggaaaataa ccatcaccga
     5341 ggatcctgag atttctaatt ccgttaattc ttcctggaaa gtgatgcggt gatggtaagc
     5401 cccatgactg gagctgtcat ggtgggctca gccacagtat caggacagat cctggccctc
     5461 cgccacaccg ggcgttggtt ccggaactca taccccatca atatatttcc ggtcgctgat
     5521 ggtggtggat ggataaacac caggaactgc ggaactggcc gccgctgcca gaagaaataa
     5581 aactccggaa tcctgaatca aagaccatac atttccccct cgggaatcaa cggtgttaat
     5641 aaccagtcta ggttcttcag cctattcttt actgggcaac agatagatca gtaactacaa
     5701 ttgcccctca tcagacatgg tcggggtggc taggctgctt ctccaattct tatccaagca
     5761 gttttggcgt cggggagctc ataatggccg caactatcat ctgactgaac ttagtaccat
     5821 caaagtccac cgatctttta ttggtatctt caatgggtct taattggagc tggtagagct
     5881 cctccaggtt gaggtcgctg gtaatttggg ccccactttg gaacatgctg aggtatacta
     5941 tgaggtccaa atctgtgacg ttcactccac ctcttccaat ccccaaagaa gacccactct
     6001 ccaggcaatg cctgtgccgc cgcctccact taaaaccagt gctttggatt ttaagtcata
     6061 aatcttccac caactgatta ttaaaaaaag gtttctttat aaaatttctc cttgtacttt
     6121 tcatactcct tttctaatag ttccctgctg ttctcagacc catgaccggg taaaaatatc
     6181 ttacaaccag tattcaggag ttttttccat ctttggatct ggtgggtaca tcatcggtaa
     6241 atggggggaa tattccgctg aatactccaa acagggcatc acccgtggtc atagtggatg
     6301 tgggttaaaa ataggtagga taattcacca tttatcaatg gttaagtcct tgttcgaggt
     6361 cattccatga cttc
//
LOCUS       DAOQVW010000020         6258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_13799_length_6258_cov_3.559729, whole genome shotgun sequence.
ACCESSION   DAOQVW010000020 DAOQVW010000000
VERSION     DAOQVW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_6258_cov_3.559729"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKK85_00820"
     CDS             complement(<1..194)
                     /locus_tag="PKK85_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit F"
                     /protein_id="HNS24599.1"
                     /translation="MKVQELSYEQNLALDHVTKFSKISVESAQKMIAELEETIKTNLA
                     VKMADIMPEDMADMRLMFSK"
     gene            complement(272..562)
                     /locus_tag="PKK85_00825"
     CDS             complement(272..562)
                     /locus_tag="PKK85_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HNS24600.1"
                     /translation="MQRSRGSRSKTRHKLKKTIRAGRTNPITKKIQTFSKDDLVHIII
                     DPSVQKGQPHPRFHGKTGKISHKRGRAYIVEINDGDKAKNLIIRPEHLKIQE"
     gene            complement(827..2050)
                     /locus_tag="PKK85_00830"
     CDS             complement(827..2050)
                     /locus_tag="PKK85_00830"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HNS24601.1"
                     /translation="MDFILKNKATQIMELTDGEICDHCLGRHLSRELPGESNQERGEY
                     VRHNLLDGTEKLQVIEKCQICSGILDNLDKTLLNILDRVGEINVEFSTFLVGCRLPPE
                     IMDKENDLQREIQLEVESIKKEFNRELGKMLHEHWGKEVDFDNPHLVVMVDIPNNSVV
                     LQINPLFIEGRYRKLVRNIPQTKWPCRKCKGKGCAFCNYTGKMYEESVEELISPPVLK
                     ATKGSESKFHGAGREDIDVKMLGNGRPFVLEVKEPLKRSLDLKSLEEDINRYGQGKVE
                     VLKLKPSQKQRRGAIKNSSTDTYKTYLACVELDEAVDAVNLDSLSKLKLIKQRTPIRV
                     SHRRADKVRKRRIKELTFTKIDEKTLELIIDCEGGLYIKELISGDEGRTQPSVASLLG
                     IPARCVQLDVMEVNI"
     gene            complement(2097..3431)
                     /locus_tag="PKK85_00835"
     CDS             complement(2097..3431)
                     /locus_tag="PKK85_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein Srp54"
                     /protein_id="HNS24602.1"
                     /translation="MLGNLGKNLTNTMKKLAGMAIIDEEVVKEAIKDIQRALIQADVN
                     IKLVLNLSKTIEKRALSEKPPKRVTPKEHVIKIVYEELVHLLGEKAPEVEVESKPFKI
                     LFMGLQGSGKTTTIGKLARYLQKKGFNPAVVCTDTWRPAAYEQLRQLTESLSIPLYGD
                     PDNEDALDLAKKGINKFKKQDIIIVDTAGRHKEEQDLLDEMKQISTVVDPDEVMLVID
                     GTIGQQAREQALAFSQTTDIGSITITKLDGSAKGGGALSAVAEIGAPIKFIGTGERIE
                     DLEAFDPDRFISRLLGMGDIKTLIERAEEIAEDDEVDMESMDAMLSGKFTLKDMYAQF
                     EVMNKMGPMQQILNMMPGMGSKLPKNASQVTEERLNKYRVLMDSMTEHELTHPEVIKQ
                     SRVKRIAKGAGMRNEDVKELLKYYQVTKKAIKGFGKRKMGGPLGQMKRQMMR"
     gene            3754..4308
                     /gene="hpt"
                     /locus_tag="PKK85_00840"
     CDS             3754..4308
                     /gene="hpt"
                     /locus_tag="PKK85_00840"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine/guanine phosphoribosyltransferase"
                     /protein_id="HNS24603.1"
                     /translation="MLEKLKKTLIESPIVIKDDYNYFVHPITDGIPLTEPELLEEVAD
                     AISRIMSFPLDKIVCVEAMGIHLATALSIKTRIPFVVVRKRRYNLDGEVAVHQMTGYS
                     EGELYINGLTKNDRIILIDDVVSTGGTMTAVLKALQNMGLEILDVVAVIEKGSGKERV
                     EAETGLEVKTLVKVDVQDGEVVIL"
     gene            4352..5344
                     /gene="dph2"
                     /locus_tag="PKK85_00845"
     CDS             4352..5344
                     /gene="dph2"
                     /locus_tag="PKK85_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HNS24604.1"
                     /translation="MLNYQLKEEEVVAEIQELNAQMVGLQFPDGLRVHATGLARKIEK
                     ETGATTLISGDSCYGACDLSDRSMEGLVDVLIHWGHTALPLDYRVPVLFVEAYSTMEV
                     FGSVKKALKFIEDYETIGLVTTTQHLHLLDDLAKYLEENGKKVQMGVGKGTLRGQVLG
                     CNFSSASGLRADAFLYIGSGNFHPLGLKLSTNKPVIIADPYMNEARIIDEFTDRILRK
                     RFAKITLSAGAKSFGIIVSSKEGQMRLEMAKSLKKLIQDQDREAYLILLDNIRPDLLM
                     AFRDLDVFVVTACPRIAIDDDQIYEKPLITPYELEIALGIRDWEDYQLDEIIYE"
     gene            5665..6234
                     /locus_tag="PKK85_00850"
     CDS             5665..6234
                     /locus_tag="PKK85_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Csl4"
                     /protein_id="HNS24605.1"
                     /translation="MKAKSGDFVLPGDALGVTEEFLPSEWTYEEEGKIRSLVAGTVSI
                     DQKNKKISIIPKTKSPAILSRGVVILGQIKDVRGQRAMVEIEGIKDTNRSLPISCVGG
                     IHISQAQKGYVDKLTDDFRIGDIVEAKVTKVLGIDNADLTTADDELGVLKAMCTKCRY
                     FMNKIGKNQVKCPNCGNKETRKLSSKYEG"
BASE COUNT         1797 a         1244 c         1231 g         1986 t
ORIGIN      
        1 tctttagaga acatgagcct catatcagcc atatcttcag gcattatatc tgccatttta
       61 acggctagat tggtttttat agtttcttct aattcagcaa tcattttctg ggcagattct
      121 accgaaattt tggaaaattt ggtaacatga tccaaggcca gattctgctc gtaactcagt
      181 tcttggacct tcattctctc ttccaacaac tctttaactt ggcaaaaaga tattggatca
      241 gtttctaaca ctttttttcc aatcatccta atcactcttg gattttaaga tgttcgggcc
      301 ttataatcag attttttgct ttatctccgt cgtttatttc cacgatatag gctctacctc
      361 gtttgtggga tattttacca gtttttccat gaaaacgggg gtgaggttga cccttttgta
      421 cacttggatc tattatgata tgaaccaagt catctttact gaaagtctgg atttttttgg
      481 taatgggatt ggttctaccg gctctaattg tttttttgag cttatgtcgc gttttacttc
      541 tggatcctct tgatctttgc atttaaaaac ctccatgata aactagaact ttaaagaaat
      601 tagttgttca ttggtgagtg aaactgaatt ctaacaagtt ccaacatctt tcacaacttt
      661 tatcaactaa tagtacccta actaaccatc agatgacatt catgctgatc aagcagttat
      721 taccctgtat ctgccctaat ttggctgaaa tgggctgggc atcttctatt gtctacgttg
      781 aataggtata catttcagga gtttataata ctatccttcc acctttttaa atgttgacct
      841 ccatcacatc caactgcaca catctggctg gaatgcccaa aagtgatgcc acactaggtt
      901 gggttctacc ttcatcacca gatatcagtt cttttatgta caagccacct tcacagtcaa
      961 ttatcaattc cagggttttt tcgtcaatct tagtgaaggt caactcctta attcttcgtt
     1021 ttctaacctt atcagcccgg cgatgggaca ctcggatggg ggtgcgctgt ttgattaatt
     1081 tcaatttaga tagggaatct agatttacgg catcaactgc ttcatccaac tctacacaag
     1141 ccaggtaagt tttataagta tcggttgagg agtttttgat ggcacctcgg cgctgttttt
     1201 gggacggttt aagtttcaga acctctactt tgccctgtcc atacctattt atatcctcct
     1261 ccagactttt aaggtctaaa ctacgtttta agggttcttt aacttccagt acgaatggtc
     1321 gcccgtttcc caacatttta acatcaatat cttcccttcc cgcaccatgg aacttagatt
     1381 cacttccttt cgtagctttt aagaccggag gagatatcaa ctcctctaca gattcttcat
     1441 acattttccc ggtgtaatta caaaatgcac aaccttttcc tttgcacttt ctacaaggcc
     1501 attttgtttg gggaatattt ctcaccagtt tccggtagcg cccttcaata aagagtggat
     1561 taatctgcag aacaacacta ttattgggga tgtcaaccat caccaccaaa tgggggttgt
     1621 cgaaatccac ttccttcccc caatgttcat ggagcatttt acccaactca cggttgaatt
     1681 cttttttaat actctccact tctaactgta tttccctttg caaatcattt tctttatcca
     1741 ttatctccgg gggaaggcga caaccaacca agaatgtaga aaactcaaca tttatctccc
     1801 caaccctatc taaaatattt aatagggttt tatcaagatt atcaagaata ccagaacaaa
     1861 tctggcattt ttcaataacc tgcaattttt ctgttccatc taagagatta tgccgaacat
     1921 attcgcctct ttcctgattt gactctccag gtaactccct ggagaggtga cggccgaggc
     1981 agtggtcaca tatctcacca tcagttagtt ccatgatctg agtagcctta tttttcaaaa
     2041 taaaatccat aaaaatccac ccaataatga tataattaaa ataaaaaaaa tgaaggtcaa
     2101 cgcatcatct gcctcttcat ctgccccaag gggccgccca ttttacgctt accaaatcct
     2161 ttaatggcct ttttagtaac ttgataatat tttaaaagtt ctttaacatc ttcgttacgc
     2221 atacctgccc cttttgcaat tcgtttaact cgggattgtt ttattacttc aggatgagtg
     2281 agttcgtgtt cagtcatgga gtccataaga acacgatact tgttgagtct ttcttctgtt
     2341 acttgggatg catttttagg taacttgctt cccatcccgg gcatcatgtt aaggatctgt
     2401 tgcatgggac ccattttgtt cataacttca aactgggcgt acatatcctt gagggtaaac
     2461 tttccactga gcatggcatc catggattcc atatccactt catcatcctc ggctatttcc
     2521 tctgccctct ctatcagggt cttaatatct cccataccca aaagtcggga gatgaaacgg
     2581 tcgggatcaa atgcctccag atcttcaata cgctcaccag ttccaataaa cttgatgggg
     2641 gcacctattt cggccacggc tgaaagggct ccaccccctt tagctgatcc atcgagtttt
     2701 gtgatggtga tagagccgat atcggtggtt tgactgaaag ctaaagcctg ttcacgtgct
     2761 tgttgaccaa tagtgccatc gataaccagc atcacttcat ctggatcaac caccgtcgat
     2821 atctgtttca tttcatccaa aagatcttgt tcttctttat gacggccagc tgtatctaca
     2881 atgatgatat cctgtttttt gaatttatta atgccttttt tagctaaatc caatgcatct
     2941 tcattgtcag gatctccgta aagtgggatg ctgagacttt cagttaactg gcgaagttgt
     3001 tcatatgctg ccggcctcca ggtatcggta catacaacgg cggggttaaa accttttttt
     3061 tgaagataac gggctaattt tccgatggta gtagtttttc cacttccctg caatcccata
     3121 aagagtattt tgaagggttt gctttctacc tcaacttcgg gagccttttc acctaaaaga
     3181 tgtaccagtt cttcatagac gatcttgatc acatgttctt tgggagtcac tctcttaggg
     3241 ggcttttctg acagagcacg tttttcaatg gttttggaaa gatttaaaac cagttttata
     3301 ttaacatcag cctgaataag ggctctttgt atatctttta tggcttcttt gaccacttct
     3361 tcatcaatga tggccattcc cgccagtttc ttcatagtat tggtcaggtt ctttcccaga
     3421 ttgcctaaca ttgcatatca ccagattaga ttcatcacca gattgggcac cattaattga
     3481 taaaaaaaaa tcagcatata aatttaattc tgaatattga gtatatatac taaattctgg
     3541 gtatataaag atattcagtg tggaaccttc tgcactgaac gaaaaattga tgggggactc
     3601 tttagactta aacagcctta tttgtgaaca tgttccggtt aaaatatctt gatgtttaca
     3661 gtggatatgt tttatatttc aagtttatat cctccttttt ataattattt taattagaac
     3721 caggagcaac ttgtattaat ttatggtgaa aaaatgctgg aaaaacttaa gaagacttta
     3781 attgagtctc ccattgtaat aaaagatgat tataactact tcgtacatcc cattaccgat
     3841 gggattccct taactgagcc agaattactg gaagaagttg ctgatgctat ttctcgtatc
     3901 atgagttttc cattggacaa gatagtttgt gtggaggcta tgggcatcca cctggcaacc
     3961 gctttatcaa ttaaaaccag aatcccgttt gttgtggtaa gaaaacgtcg ttacaatttg
     4021 gatggggaag ttgccgtcca tcaaatgacg ggatacagtg aaggagaact ttacatcaat
     4081 ggtttaacca aaaatgaccg gataatcttg atagatgatg tggtaagtac tggtggtacc
     4141 atgactgcgg tgttaaaagc ccttcagaac atgggattgg aaatccttga tgttgtggcg
     4201 gttattgaaa aaggaagtgg gaaagaaagg gtagaggctg aaactggact ggaagttaaa
     4261 accctggtta aagtcgatgt acaagacggt gaagtcgtga ttctataaac tggctatgga
     4321 ggataggggt aagaacccca tgggaactaa aatgctgaat tatcaactga aagaagagga
     4381 agtagttgct gaaatacaag aattaaatgc tcaaatggtt ggattgcagt ttccagatgg
     4441 attgagggtt catgccacgg gattggcccg aaaaatagaa aaggaaactg gagctaccac
     4501 tctaatatcg ggagattcct gttatggggc atgcgatcta tctgatcgtt ccatggaagg
     4561 tttggtggac gtgttaattc actgggggca cactgctcta ccattggatt atcgggtccc
     4621 ggttttattt gtggaagctt actccaccat ggaagtattt ggatcagtta aaaaggctct
     4681 taaattcatt gaagattatg aaaccattgg tttagtcacc actacccagc atctccactt
     4741 gttggatgat ttagctaaat acctggagga aaatggtaaa aaagttcaaa tgggagttgg
     4801 taaaggaact cttcgtggac aggtgctggg ttgtaacttt tcttcagcta gtgggctccg
     4861 ggcggatgct ttcctttaca taggcagtgg aaacttccac ccgctaggtc tgaaactatc
     4921 caccaataaa ccggttataa tagcagatcc ttatatgaat gaggccagaa taatagatga
     4981 attcacagat cggattttga gaaagcgatt tgcaaaaatc acactatcag cgggggctaa
     5041 aagttttgga atcattgttt catcaaagga aggccaaatg cgcctggaaa tggctaagtc
     5101 tttaaaaaaa ttaattcagg atcaagatag agaagcttac ctcatactcc tagataacat
     5161 aagacccgac cttttaatgg catttaggga tttggatgtc tttgttgtga cggcatgtcc
     5221 acgaatagcc attgacgatg accagatata tgaaaaaccc ttaattactc catatgaatt
     5281 ggaaattgca ttgggcataa gggattggga agactaccaa ttagatgaaa ttatatatga
     5341 gtaagaatga gagaataggg agggaacttt ttggagtttt cagatgatta cctactaggt
     5401 tatatcaact gagtcctaaa ttgatatcat tgcttaatta ttttaatttg aatgcacatc
     5461 tatggttaag ggaaaagata taatcacttg gagcatatga tataatcgaa gttatcaatt
     5521 cttgactcac accaataatt aatattacat gcacatgcta atcagtatca gcccattatc
     5581 ctaatgggaa ccatatcctt atacatcaaa attatttatt aagtacgaat tgtttagact
     5641 tgttttacaa tacgaggtga atagatgaaa gccaaatctg gagattttgt tctccctggt
     5701 gacgctttag gtgttactga ggagtttctc ccatctgaat ggacctatga agaagaaggt
     5761 aaaattcggt ctttggtggc aggaacagtg tccatagacc agaaaaataa aaaaatttcc
     5821 ataataccca aaactaaatc ccctgctatt ctcagtagag gtgttgtcat tttggggcag
     5881 attaaggatg tgcgtggtca gagagccatg gtggaaatag aaggaattaa agacaccaac
     5941 aggagccttc ccatatcctg tgtaggcggt attcacatat cacaagccca gaagggctat
     6001 gtggacaagc ttaccgatga tttccgcatt ggtgatattg ttgaagctaa ggtaacaaag
     6061 gttctgggca ttgacaacgc cgacctcacc acagcggatg atgaacttgg agttttgaag
     6121 gccatgtgca ccaaatgccg ctatttcatg aataaaattg gtaaaaatca ggtaaaatgc
     6181 cctaactgtg gtaataaaga gactcggaag ttatcttcta aatatgaggg atgatcatga
     6241 agatcataaa ggatgaaa
//
LOCUS       DAOQVW010000021         6257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_13802_length_6257_cov_3.447275, whole genome shotgun sequence.
ACCESSION   DAOQVW010000021 DAOQVW010000000
VERSION     DAOQVW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13802_length_6257_cov_3.447275"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKK85_00855"
     CDS             <1..303
                     /locus_tag="PKK85_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HNS24606.1"
                     /translation="GEIYVKGDCDILAGIHMNKGFIQIDGDVTRWPGGQMKNGNILIK
                     GKLGILLEGFVYDSVVVDPEIDGKTFSGKYIKYIGDIAVNGKGGLYLNAEKNREFL"
     gene            393..686
                     /locus_tag="PKK85_00860"
     CDS             393..686
                     /locus_tag="PKK85_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2097 domain-containing protein"
                     /protein_id="HNS24607.1"
                     /translation="MEEEIFLKADETIDYLKENVETHDILEISYNRIYAPGEVLGIQM
                     EEEHGEEFLQLTLHLTGELVNQTVRINMHAIKDDLIEIIHTHGEDSRIIVVED"
     gene            719..1039
                     /locus_tag="PKK85_00865"
     CDS             719..1039
                     /locus_tag="PKK85_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2097 domain-containing protein"
                     /protein_id="HNS24608.1"
                     /translation="MVKELTMDCEEVIDYVKKNVKAYDNVELSYNRVFTPGEVINIET
                     CVLKGGKKSCTILVQLDGDTIHSTVDIDLEEIKYDLIEVKHIPQDGDETLITIDTCEE
                     EFEE"
     gene            complement(1048..1740)
                     /locus_tag="PKK85_00870"
     CDS             complement(1048..1740)
                     /locus_tag="PKK85_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNS24609.1"
                     /translation="MDRSKEGPQYRLKFDESIILVNKKKFKLLKFIDECNSIMEASKN
                     ANIPYRSALKYIEDLEKEINRRIVATQRGGKGGGGESKLTDAGKSILKEYRKVESILK
                     MHADVNEIEGNIEEIDVENKIANIHLNGKKVVLPLRGNFKVGDRVLVLISPDDIFVML
                     EPHESSVRNIFPGRIVGMELKDNLVRLNVNLGNTSLFADITEYAREQLNLTLGKEIYI
                     GFKAAALAMVKI"
     gene            complement(1840..2940)
                     /locus_tag="PKK85_00875"
     CDS             complement(1840..2940)
                     /locus_tag="PKK85_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS24610.1"
                     /translation="MFLEVKNLCVDLGQFHLTGVNLNLEKNDYMVIIGPTGSGKSVLL
                     ETIAGFFQPQTGKIFLEGEDITNLNPEKRGISIVYQDYVLFPNMNVFENIAYGLEKKV
                     KDREVIKSRVYDMADRLKIEHLLDRNPETLSGGEKQRTAIARSLIVEPNILLMDEPFS
                     ALDVNTHAYLTELIKKAISDYQTTCIHVSHNFNDVWSLAEQVAVMKNGKILQQGIVAD
                     VFSKPCHNFVADFVGVHNIFEGEVVGKSKGITDVKVNQNLILHSSDTDTNDDKKVMVA
                     VRPENIIFSNQSFVSSIRNQLKGIVTDIILIGPTVWVEVDVNGTTFKGIITPSSLDIL
                     GIKKEKEIYLSFKSVNVKIIDEEQSYSALFSA"
     gene            complement(2946..3593)
                     /locus_tag="PKK85_00880"
     CDS             complement(2946..3593)
                     /locus_tag="PKK85_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS24611.1"
                     /translation="MRLTLSTSIIAAFGVMIVAIPTAYSLARYNFPFKSAVKSIIDLP
                     MAFPEIVLGIAILMLFGNPLIGSILHSLGINIVFTTTGIIIAQFFVAFPYSVRILYST
                     FNYVNPRYEFVSRSLGYGELETLKNITLPLAKGGIFASSVVTIARCIGTFGAVLLVGG
                     GTYLKTETLPIAIYYNLSLGSIDNAITAAIILVLISFIAIFVLEKYAYQNIEGAG"
     gene            3568..3780
                     /locus_tag="PKK85_00885"
     CDS             3568..3780
                     /locus_tag="PKK85_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24612.1"
                     /translation="MEVDKVNLKANCISSEKNAFTNPSNEGITNKLPIIRNKILVKIK
                     DMDMKDISNLLLIFSSLFFISDEVYD"
     gene            complement(3809..4606)
                     /gene="modA"
                     /locus_tag="PKK85_00890"
     CDS             complement(3809..4606)
                     /gene="modA"
                     /locus_tag="PKK85_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNS24613.1"
                     /translation="MNSKNIAIIGIIAIVIIVAGLYASGMLTGGNAQGNITVLAGAGT
                     MSAMNELKAAFEEKYPGTTVNVQYGNSAEIFSMLETQKSADLVVPGDLVFMDNAKKKG
                     YIVNDTITSLVYHIPVIGVQKGNPKNITQLSDLGQSGLKVGLGDVNGTAVGKQAIKLL
                     NKTGNLEAVKTNVVVYAPTVNQLMTYLTSGQVDAAIVMNDTASKAAAEGKIDIITIPE
                     DQNEIGTIGVALTIFSKNKNTAQTFLEFVSSPEGMAIWEKNGFKPVK"
     gene            complement(5296..5706)
                     /locus_tag="PKK85_00895"
     CDS             complement(5296..5706)
                     /locus_tag="PKK85_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24614.1"
                     /translation="MKDGKISQVGTSTEIFRQPADNFVAEFVGAENIIECIAKKDGEK
                     LTLIDTGNISIYSTELKRGHVHITVRPEDIILSTKQVETSARNVFKGNIIEIVDTGAI
                     IKLTIDVGEPIVVFLTRQSFLDRTKYWEECVDLF"
     gene            complement(5786..>6257)
                     /locus_tag="PKK85_00900"
     CDS             complement(5786..>6257)
                     /locus_tag="PKK85_00900"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS24615.1"
                     /translation="SLSIAMARITELKIYAMDISPEMYKIAGKNIAKCGFKDRILPML
                     ADVHQMPFPDNYAHLIFSRGSMFFWKDLASALKKIYAVLKPGGASYIGGGFGNKTTKK
                     LVKKTFKNQEENYESPPKIDIATLEIAVQKAEINNYQLLDDESGLWVLFEKSIE"
BASE COUNT         1881 a         1156 c         1153 g         2067 t
ORIGIN      
        1 ggagaaatct atgtaaaagg agactgcgac atcttagcag gaatccacat gaacaaagga
       61 ttcatccaga tcgatgggga cgttacccgc tggccaggtg gtcagatgaa aaacggtaac
      121 atccttatca aaggaaaatt gggaattctc ctggaaggat ttgtctacga cagtgtcgtt
      181 gttgatccag aaatcgatgg aaaaaccttt tctggaaaat acatcaaata cattggtgac
      241 atagctgtaa atggtaaagg tggcctctac ctcaacgcag aaaaaaacag agaattctta
      301 taatctctgt tcacccattt tttttaattt ttttttcaaa aacaaaattt tttttaacag
      361 gttactgtat aacaatttat caagtgattt ttatggaaga agaaatattt ctgaaggccg
      421 atgagactat agactatctc aaagagaatg ttgaaaccca tgatattctt gaaatttcat
      481 ataatcgtat ttatgctcct ggagaggttt tagggattca aatggaagaa gaacacgggg
      541 aagaattcct tcagcttact ttacacttga ctggagaact ggtcaaccag acggtgcgaa
      601 taaacatgca tgccataaag gatgatctta ttgaaataat acatactcat ggagaagatt
      661 ccaggattat tgtggtggaa gattagtgga taaaatttga tgtagtgggg tgattatgat
      721 ggtgaaagaa ttaacgatgg attgtgaaga agtgatcgat tacgttaaaa agaacgtaaa
      781 ggcctatgac aatgttgaac tgtcctacaa tcgagttttc actccaggtg aggtaataaa
      841 catagagacc tgtgtgctca aaggcggaaa aaagagctgc acaatactag ttcaactgga
      901 tggggacacg attcatagta ccgtagatat tgatcttgaa gaaataaaat acgatttaat
      961 tgaagttaaa cacatccccc aggatggtga cgagactcta atcaccatag atacttgtga
     1021 agaagaattc gaagaataat gtggtgttta tatcttaacc atggctaatg ctgctgcttt
     1081 aaaacctatg taaatttctt ttcccaaggt caggttaagt tgctcccggg catattctgt
     1141 gatatcggca aaaagtgagg tattgccgag atttacattt aaacgcacta gattgtcctt
     1201 gagttccata cccacgatcc tccctggaaa tatgttccgt acgctggact cgtggggttc
     1261 cagcatcaca aaaatatcgt caggacttat tagtactagg actcgatcac cgactttgaa
     1321 gtttcctcgc aggggaagaa ccactttttt tccatttaaa tgaatgtttg caattttatt
     1381 ctctacgtcg atttcttcaa tgttaccttc aatctcatta acatcggcat gcattttcag
     1441 aatgctctct actttcctgt attcttttaa gatagatttt cctgcgtcgg tgagtttact
     1501 ttcaccacca ccaccttttc ctcctctctg ggtggctaca attcgcctgt taatctcttt
     1561 ttcaagatct tcaatgtact tcaatgcact tcgatagggg atatttgcat ttttagaggc
     1621 ttccataata gagttacatt catctatgaa ctttagaagc ttgaatttct ttttattaac
     1681 gagaattatt gattcgtcga attttaatcg gtattgtggt ccttcttttg atcggtccat
     1741 gaaaagtcct ctaatcaaat attactttaa atttatattt agtctaataa aaaattttcc
     1801 ggaggtattg gaaataaaag attcccagtg gcttctattt caagcagaaa atagtgcact
     1861 gtaagattgc tcctcgtcta ttattttgac gttaactgac ttaaaactta aatatatttc
     1921 tttctctttt tttattccca gtatgtccag tgaactggga gttattatcc ctttaaaggt
     1981 ggtgccgttc acatctacct ctacccaaac tgtggggccg atgagtatga tatcagtaac
     2041 tatgcctttt aactggtttc tgatggacga tacaaatgat tgattggaaa atattatatt
     2101 ttcaggtcgt actgctacca ttactttttt atcatcatta gtatcagtat ctgaactatg
     2161 caaaatcaga ttttgattaa ctttcacatc agtgatgcct ttactcttcc caaccacttc
     2221 tccttcgaag atgttgtgaa cacctacaaa gtcagccaca aaattatgac aaggtttaga
     2281 gaaaacatct gctactatac cctgctgcag gatcttaccg tttttcatca ctgccacttg
     2341 ttcagctaaa ctccagacat cattaaagtt atggcttaca tggatacagg tagtttgata
     2401 atcgctgatt gcctttttta ttaactctgt cagataggcg tgggtgttaa catccagggc
     2461 actgaatggc tcatccatta acaggatgtt gggttcaaca ataagggacc tagcgatggc
     2521 tgttcgctgt ttttctccac cactcagggt ttcaggattt cgatccagta aatgttcaat
     2581 tttgaggcga tcagccatgt catatacccg ggattttata acttctctgt ctttaacttt
     2641 tttctcaaga ccataggcaa tattttcaaa tacattcatg ttggggaaaa ggacgtagtc
     2701 ctggtagaca atgctgatgc ctcttttttc ggggttaagg ttagtgatgt cttcaccttc
     2761 caggaaaatc ttccctgttt ggggctggaa aaaaccagct attgtttcta acaacacgga
     2821 ttttccagag ccggtgggcc caattataac catgtaatcg tttttttcta aattaagatt
     2881 cacaccagtt aagtgaaact gacccaagtc cacgcataaa tttttcactt ctaaaaacat
     2941 gagatctaac ctgctccttc gatgttctga taagcgtact tctccaggac gaaaatggct
     3001 ataaaggaaa ttaaaactag tattatcgcc gctgtaatgg cattatctat acttcccagg
     3061 gatagattat agtaaatagc aatgggtaga gtttctgttt ttaaataagt tccaccaccc
     3121 accaataaga ctgctccaaa ggttccaata catcttgcta tggtgaccac tgaagaagcg
     3181 aatataccgc ctttagcgag tggtaaagtg atatttttta atgtttcaag ttccccgtaa
     3241 cctaagcttc tggaaacaaa ttcataccta ggattcacgt agttgaaggt ggagtacaat
     3301 attcgcactg agtaggggaa ggccacaaag aattgggcaa tgatgatgcc ggtggtggtg
     3361 aacacaatgt taattcccaa agaatgcagt atactcccga ttaaaggatt accaaagagc
     3421 attaatatgg caatacccag cacgatttct ggaaaggcca tgggaaggtc tataatgctt
     3481 ttcactgcac ttttaaacgg aaaattgtat ctggccagag aataagcggt gggtatcgcc
     3541 actatcatca caccaaaagc agcgattatg gaagtggata aggttaatct caaagcgaat
     3601 tgtatctcat cagaaaaaaa tgcatttaca aatccttcga atgagggaat aacaaataaa
     3661 ctaccaataa taaggaacaa aatactggta aaaataaaag atatggatat gaaggatatt
     3721 tccaacttac ttctcatttt ttcgtctttg ttttttattt cagatgaagt ttacgattaa
     3781 aatggaacac catcatatcc tattgatatt acttcaccgg tttgaaaccg tttttttccc
     3841 agatagccat tccttccgga gaagatacga attccaggaa tgtctgggct gtatttttgt
     3901 ttttgctgaa gatggttagt gccactccga tggttccaat ttcattctga tcttctggaa
     3961 tggtgataat atcgattttg ccttcggctg cagctttgga cgcggtgtcg ttcattacta
     4021 tggcagcgtc tacttgtcca gaggttaggt aggtcataag ctgattcacg gtaggcgcat
     4081 agacaaccac attggtttta actgcttcca gatttccagt tttgttcaat agtttgatgg
     4141 cttgtttacc tactgctgtg ccgttaacat ctcccaagcc aactttaagt ccagattggc
     4201 ctaaatctga caattgagtt atgtttttag gatttccttt ctgaacacca atgaccggga
     4261 tatgatacac taaggaagta attgtatcgt ttacaatgta acctttcttt ttagcgttat
     4321 ccataaatac taaatctcca ggtaccacga gatcagcact tttttgagtt tctaacatgg
     4381 aaaaaatttc ggcactgtta ccatactgta cgttaacagt cgtaccaggg tatttttctt
     4441 cgaatgctgc tttcaactcg ttcatagccg acatagttcc ggctcccgcc aggactgtga
     4501 tgtttccttg tgcattccca ccagttaaca ttccgctggc atacaatccg gccactatga
     4561 tcactattgc tataattcca attattgcaa tatttttaga attcattttc aacctccaca
     4621 aaatgttcta acactaagat actagaatta actagaaatg catatatata catttcgcat
     4681 acaactcaaa ttagaattag caaaaatatt tgattatatt aatttttatt agcaattaga
     4741 aatatttcac gatacacgaa ttttttacaa tcgctttttc ttattactgt gcataagaac
     4801 ggatatttaa atgcaattgc attgttatta tcttattcgc tttacaatgt ttaggataca
     4861 gtaagatata gaatgaaaga gaaatgaaga taatttcgtc tttttttctc aatgggtatt
     4921 tagatattag aaacatgtat taatgagacg tatagacgaa acatctcttc actctatatg
     4981 taccaacaat ttgccctatt gcagtgttgg atgtgtggaa tgaacctagt gcaaaggaca
     5041 aatggtatgg ttgaccaagt tcaactcttt cgaaggtaat tgataatata atgaaatgca
     5101 tatataaaca tatcgaaata tgaagttatt ttctaaagtg tgataattgc aaagatttga
     5161 tttgagcagt tttgattggt tattttatgg atatcataaa aaatatcaca cgatctattg
     5221 gaatgaaata cattttttaa gaagatttag agttaaaaga tctatcgaga ctaaaataca
     5281 tgtaccgccg ttgctttaaa ataggtccac acactcttcc caatatttag ttctatctaa
     5341 aaaagattgt ctagttaaaa agacaacaat tggttcccca acatcaatgg ttagctttat
     5401 aatagcaccg gtatccacta tttctattat gtttcctttg aatacatttc ttgcactggt
     5461 ttctacctgt tttgtggata gtataatatc ttccggcctt actgtaatat ggacatgtcc
     5521 tctttttagt tcggtggagt atattgaaat attaccagta tcgattaaag ttaatttttc
     5581 tccgtctttt ttagcaatac attcgattat attttctgct cccacaaatt ccgccacaaa
     5641 attatcagct ggttgtctga aaatttcggt ggaagttcct acctgagaga ttttaccgtc
     5701 tttcatgatg gcgattcgat cagcaagtat caatgcttcg tcgaagttac gcgtcacgtg
     5761 aatgaaggta atggcaaatt ttattttatt cgatactttt ttcaaataaa acccataatc
     5821 cggattcatc atctagtaac tggtaattat taatttctgc tttttgaact gctatttcca
     5881 gagtagctat atctatttta ggaggactct catagttctc ttcttggttt ttaaaggttt
     5941 ttttgactaa tttcttcgtg gttttgtttc caaatcctcc ccctatataa gaagctcctc
     6001 ctggctttaa aaccgcatat atctttttta aggcggaggc aaggtctttc cagaagaaca
     6061 tggatcctcg actgaaaata agatgggcat aattgtcggg aaatggcatc tgatggacat
     6121 cggccagcat cggaagtatt ctatctttaa aaccacattt agctatgttt ttcccagcga
     6181 ttttgtacat ttccggtgaa atgtccattg catatatttt tagttctgta attcgtgcca
     6241 tggctataga aagagat
//
LOCUS       DAOQVW010000022         6239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_13862_length_6239_cov_3.612387, whole genome shotgun sequence.
ACCESSION   DAOQVW010000022 DAOQVW010000000
VERSION     DAOQVW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13862_length_6239_cov_3.612387"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PKK85_00905"
     CDS             complement(<1..179)
                     /locus_tag="PKK85_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HNS24616.1"
                     /translation="MLKEAKLALEDGTIFKGRPFGFETIKTGEVVFTTGMTGYVESLT
                     DPSYKGHILMSTYPL"
     gene            complement(327..761)
                     /gene="rimI"
                     /locus_tag="PKK85_00910"
     CDS             complement(327..761)
                     /gene="rimI"
                     /locus_tag="PKK85_00910"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNS24617.1"
                     /translation="MIIREFRRPDVKEVLEIEFSSFKDPYPSHILVDIYNLGAGFLVA
                     QQDNMIVGYIIFWIRFEDEGHIISLAVDKKYRRRGVGSTLVNSALDIFKKYSLKKIKL
                     EVRIGNKAARKFYQKTGFLEKEFLLNYYDDGEDAVVMEMTFP"
     gene            784..1173
                     /locus_tag="PKK85_00915"
     CDS             784..1173
                     /locus_tag="PKK85_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HNS24618.1"
                     /translation="MWSDFSEYIIQHYSHAHRIVEVGVGRFYPVALDLQRRLKVNIIR
                     TDIKPCHQDVVQDDITHPDLKIYKGAELIYSIRPPEELHPYLQKVAGDVGADLIIKPF
                     YTDTINTPKKMKLINYKRAVFFQRCSL"
     gene            1170..3695
                     /locus_tag="PKK85_00920"
     CDS             1170..3695
                     /locus_tag="PKK85_00920"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HNS24619.1"
                     /translation="MKWLKLSVDETLKNLNSTRRGLSAAEAQKRNMEYGKNELVEEAK
                     TGPIRLFLGQFMDILILILIIAAGAAYYVGNTLDAIVILIVVLINAVVGFVQEYRAEK
                     AMEKLKGLISSEAVVIRDGKEQKISAGDLTLGDVVLLEEGDNVPADMRIIESHDLLVD
                     ESTLTGESLPQEKHTEAILEDGHSPANMVFMESDISSGRGKGVVVEIGMNTEIGKIAE
                     MIQEEDEETPLQQKIARLGKILGLLALVVCTIVFIITYWQGTPLVDTFMTAVSLAVAA
                     VPEGLPAILTLTLALGMQRMARSNAIVRKLLAVETLGSCNFICTDKTGTLTLNQMTVR
                     ETKTNKLGMVYMISALCNNATISKGQVIGDPTDAALLLYANEAGYSRQELEQIHPRLL
                     EIPLDSTRKRMTTVNQIDGNRFILVKGAPEVLIQQCSHVEGGNRAYSITPEDNNLIME
                     DLKHMTDNALRVLGFAYRKLDPSEDLSDKESLETNLIFAGLVGMMDPPRQEAKDAIAI
                     ANKAGIKVVMITGDHKDTAVAIAKEIGIVGADNDIILTGSELDVLSDQEFMEVVDDVR
                     VYARVFPEQKVRIVETLKKNGNVASMTGDGVNDAPALKKASIGVAMGSGTDVAKESAD
                     MLLQDDNFATIVKAVREGRTIFDNIRRFVRFQLSTNIGAILTITSSSIIGLPVPFNPI
                     QVLWINIIMDGPPAQSLGVEPPEKDVMERPPLKKEIMSGQNLIKIIIAGVVMTTGTLA
                     LYYFLLSSGTGLIKAMSMAFTVFVMFQIFNVFNCRSAQGFSNRFLIIAVTSSFFLQLA
                     VIYTPFLQGIFKTTSLSGMDWMIVLLIASTILVSDFIVGRVLK"
     gene            3692..4474
                     /gene="cobK"
                     /locus_tag="PKK85_00925"
     CDS             3692..4474
                     /gene="cobK"
                     /locus_tag="PKK85_00925"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HNS24620.1"
                     /translation="MRIMVMAGTSDATVAIKRLKSLQDDIYILATAVTSYGAEIARSA
                     GADEVISKPLPLDDLIGIIEKHKIDVLVDSTHPFAAEATRNAIQASKETGITYLRLER
                     PPTQLPENVLIHQVTSFPEAAHLAKTISNGRILHLAGVSTLHYLTEVVDADKIVARVL
                     PNPYSVKKSLETGLPGENIIAMQGIFTPEFNKALIREYGAKLVVTKDSGEVGGTPSKI
                     EAALELEIPVIVVLRPEISELDQEKVSSSVDDMIKEIVLRMD"
     gene            complement(5016..5657)
                     /locus_tag="PKK85_00930"
     CDS             complement(5016..5657)
                     /locus_tag="PKK85_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNS24621.1"
                     /translation="MKKMVFCNNCGNENIDNAIYCSECGEKLQNISQDQIETNEELLD
                     KEILLELMHVSNDANTHKLLNRDIPNPSRDAKRSLYFTNENIYIGKGTALGNYEMVGS
                     MADTAGWMTGGLSGYLIGKKAAKKYYSDLEQKNKQINFKELAAKDPSVRVIPHSDIIN
                     VIMDKPKTLRPSYITIKTSTEDYRFVSQEPKKYKKQFIKTIPAILGDRVIIGK"
     gene            complement(5939..>6239)
                     /locus_tag="PKK85_00935"
     CDS             complement(5939..>6239)
                     /locus_tag="PKK85_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876367.1"
                     /note="required for the ATP-dependent degradation of
                     proteins by the 20S proteasome; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HNS24622.1"
                     /translation="RFDRFIEVPVPNEDGRREILKIHTKNMVMEEDVDLDLISALTDG
                     ASGADVKAICTEAGMFAIREERPIVNINDFLDAVDKIMGVERDEEIRKEAGVMYG"
BASE COUNT         1848 a         1257 c         1285 g         1849 t
ORIGIN      
        1 tgcagaggat aggtggacat taatatatgt cctttgtaag atgggtcggt caaagattcc
       61 acatacccag tcattccagt agtaaatact acttctcctg ttttaatagt ctcaaaaccg
      121 aatggtcttc ctttaaaaat agtgccatct tctagagcca gttttgcttc tttaagcatg
      181 ccatcaccat gttattatca tctgtttagt tcccttcacc tactgattat tagatactat
      241 gccgcccaat tttatttttt ttatcacaaa ttttcctgtc ttaaattgaa tcgtaactgg
      301 atttatcaaa ttcgaagttt ggatcattag ggaaaggtca tttccattac taccgcgtct
      361 tcgccgtcat cgtagtagtt aagtaagaat tccttttcta gaaagccagt tttctgataa
      421 aattttctcg ccgccttgtt accaatcctt acctccaatt ttatcttttt taggctgtat
      481 tttttgaaaa tatccagggc tgaattgacc agagtcgagc ccacacccct ccgtcgatat
      541 tttttgtcca cggccagtga tatgatgtga ccttcatctt caaatctgat ccaaaatata
      601 atatatccca cgatcatatt atcttgctgg gccactagaa aaccagcgcc caagttataa
      661 atatccacca gaatgtggga cgggtaagga tctttaaaag aggaaaactc aatttccaga
      721 acttccttaa cgtccggacg cctgaattct cttattatca tgactttctc cgtgagtgat
      781 gacatgtgga gtgacttttc cgaatacata atccagcact attcacatgc ccatcgtatt
      841 gtggaggtgg gagtgggacg tttttatccc gtggcattgg acctccaaag gcggcttaag
      901 gtgaatatca tcaggaccga tattaaacct tgccatcagg acgtagtcca agatgatatt
      961 acccatcctg acttaaaaat atataagggt gcagaactga tttattcgat acgacctcca
     1021 gaagaactcc acccctactt gcagaaggtg gcaggagatg ttggggcaga tttgataata
     1081 aaaccctttt acacagacac tataaacact cctaaaaaaa tgaaacttat caattataaa
     1141 agagcagtat tcttccagag gtgctccctt tgaaatggct taaattatct gtcgatgaaa
     1201 ctttgaagaa tttgaattca accagaaggg gcctatcagc tgctgaggct caaaaacgta
     1261 acatggagta tggtaaaaac gaactggttg aagaagcaaa aacaggaccc atcaggttat
     1321 ttttaggcca atttatggat attctgatac tgattctaat tatagctgca ggagctgctt
     1381 actacgtggg aaataccctg gatgccattg tcatacttat tgtagtgtta attaacgcgg
     1441 tggttggatt tgtccaggaa taccgtgctg aaaaggccat ggaaaagtta aagggcctca
     1501 tatccagtga ggcagtggtg atccgggatg ggaaggagca gaagatcagt gctggtgatc
     1561 ttaccctggg agatgttgtt cttctggaag aaggggacaa tgtccctgct gacatgagga
     1621 tcatagagag tcacgacctc ctagttgatg aatccaccct cactggtgaa tctttacccc
     1681 aggaaaaaca taccgaagcc atcttggaag atggtcacag cccggcgaac atggtgttca
     1741 tggagtctga catctcatct ggacgtggaa agggggtagt tgttgaaatt ggtatgaaca
     1801 ccgagattgg taagattgcg gagatgatac aggaagaaga tgaagaaaca cccctccagc
     1861 agaagatagc acgcctgggg aagatattag gattactggc tttagttgtc tgcactatcg
     1921 tattcatcat tacctactgg cagggcactc ctctggtgga cacttttatg acagcagttt
     1981 ctcttgctgt ggccgctgta cctgaaggat tacctgccat tttaacttta accctggctc
     2041 tgggtatgca gaggatggcc cggagtaacg ccattgttag gaaactcctg gccgtggaaa
     2101 ctctgggttc ctgtaacttc atatgcacag acaagacagg aactctaacc ctgaaccaaa
     2161 tgacggtgag ggaaactaag actaataaac taggcatggt ttacatgatt tctgccttat
     2221 gtaacaacgc caccatctcc aagggccaag ttattgggga tcccacagat gccgctcttc
     2281 ttttatatgc caatgaggca ggatacagcc gccaagaatt ggaacaaatc catccccgac
     2341 tactggaaat ccccctagac agcacccgta aaaggatgac aacggttaac cagattgatg
     2401 ggaaccgttt tattctggtc aagggtgctc cagaggtttt gatacagcag tgctcccatg
     2461 tagagggagg aaatagggct tattccataa ctcctgaaga taataaccta atcatggaag
     2521 accttaaaca tatgactgat aatgccctca gagtcctggg atttgcctac cggaagctgg
     2581 accctagtga agatttgtct gataaagaat ctctggaaac gaacttgatc ttcgctggac
     2641 tggtgggaat gatggatcct ccccgccagg aggccaaaga tgccattgcc attgccaata
     2701 aggcgggtat taaggtggta atgataacag gagaccataa ggacactgca gtggccattg
     2761 caaaggaaat aggtatagtg ggtgctgata atgatataat tctgactggg tcagaactag
     2821 atgtgctcag tgaccaggaa tttatggaag tggtggatga tgtgcgggtg tatgctagag
     2881 tgttccctga gcagaaggtg cgcatcgtgg aaaccctcaa aaagaatggt aacgtagcat
     2941 ccatgactgg agatggagtt aacgatgctc cagctcttaa aaaagcttcc atcggagttg
     3001 ctatggggag tgggactgat gtggccaagg aatctgcgga tatgctcctt caagatgata
     3061 actttgccac catagtgaag gcggtgcgtg agggaagaac catatttgat aatatccgcc
     3121 gctttgttag attccaactt tccaccaaca ttggagccat acttaccatc acatcttcct
     3181 ccataatagg ccttccagta ccattcaacc ccatacaagt cttatggata aacatcatta
     3241 tggatggacc gcctgcccaa tccttagggg tggaaccacc tgaaaaggat gttatggaac
     3301 gtcctcccct gaaaaaagag ataatgtccg gtcaaaatct tataaaaata atcattgccg
     3361 gggtggtgat gaccaccgga acactggcac tttattactt tttactgagc agtggaacag
     3421 gcctcatcaa agcaatgagt atggcattca cagtttttgt catgttccag atcttcaacg
     3481 tgtttaactg ccgatctgca caagggtttt ccaacagatt tctgataata gccgtaactt
     3541 catcattctt cttgcaatta gccgtaatat acactccatt tctacaggga atattcaaga
     3601 caacatccct ttcaggcatg gattggatga tagttctcct gattgccagt accatactgg
     3661 taagtgattt catagttggg agggtcttaa aatgagaata atggtgatgg ctggaaccag
     3721 tgatgccact gtagccataa agaggttaaa aagcttgcag gatgatattt acattttggc
     3781 taccgccgtc accagttatg gtgctgaaat agccagatca gccggtgctg atgaggtgat
     3841 aagtaagcca ctccccctgg atgatctgat tggcatcata gaaaaacaca agatagatgt
     3901 tttggtggac tctacccatc cctttgcagc ggaggcaacc cgtaatgcaa tccaggccag
     3961 taaagaaact ggaatcacct acctccgcct ggagaggcca cctacacaat tacctgaaaa
     4021 tgttctaatc caccaggtaa cttccttccc cgaagcagcc caccttgcaa aaacaatcag
     4081 caatggtcgt atactgcatc tggcaggagt atccactcta cactacctca cagaggtagt
     4141 agatgctgac aaaatagtgg cacgtgttct tcccaacccc tattctgtaa aaaaatcctt
     4201 agaaactgga ctacctggag aaaacatcat cgccatgcag ggtatcttca ctccagagtt
     4261 caataaggcc cttatcaggg aatatggtgc aaaattggtg gtaaccaaag acagcgggga
     4321 ggttggtgga acgccctcta agattgaggc tgctctggag ttggaaatcc cggttattgt
     4381 ggtcctcagg ccagaaattt cagagttaga tcaggagaaa gtatcaagca gtgtagatga
     4441 tatgattaaa gaaatagttc tacgaatgga ttagatagta tttaattaaa aataggaagt
     4501 taaattaata aaagtaagcc tcagatcgcc catcattcat catcagttca gagctcatag
     4561 ggttcatcat cggcttaatt ttttatagat ttaattgaat gtaattaata ttataatcaa
     4621 gaaagaaaat ataatttaca aattatttat tgtatttttg tataatatat tattaaattc
     4681 tatctctggt atcatgagtg ataaaaagga cagtcgctta ggcaggagat ggactcttga
     4741 agaagatatt cagatgtgta aacttcgaga aagggttaaa actttaaaga aataggtaaa
     4801 ctattaaaaa gaagtcaaaa atcttgtgca acccgttaca gcaatcgtgg ttatacattg
     4861 aatccattga agttactttt gaaccttttg attgtaatta tgtctttaat aactggtttt
     4921 gttactgaac ctatgactta attaattaaa aaaaccatca cttggaatac tgttgaggta
     4981 actggtctgt gcacataaac tttgtataac ttaattcatt tgccaataat cactctatca
     5041 cctaagatgg cgggaatggt ctttatgaat tgttttttgt atttttttgg ttcctggctt
     5101 acaaaccgat aatcctcggt gctggtttta atggttatat atgaaggtcg taaggttttt
     5161 ggcttatcca taattacatt tataatatca gaatgaggga ttacacgtac gcttggatcc
     5221 tttgctgcta attccttaaa gttaatttgt ttgttttttt gttcaagatc ggaataatat
     5281 tttttagcag cttttttacc aatcaaatat cctgatagtc ctcctgtcat ccaccctgca
     5341 gtgtctgcca ttgatccaac catttcgtaa ttacctagtg ctgttccttt acctatgtaa
     5401 atattttcat tagtaaagta tagagatctt ttggcatctc tgctagggtt tggaatgtcc
     5461 ctgtttagaa gcttatgcgt gtttgcatca ttactaacat gcatgagttc tagtaatatt
     5521 tctttatcaa gtaactcttc attggtttct atttggtctt gggatatatt ttgtagcttt
     5581 tcaccacatt ctgaacagta aattgcattg tctatatttt cattaccaca gttgttgcaa
     5641 aataccattt ttttcatcct tccattatct tactcaatat tttatcgtgg gtaagatcct
     5701 aaaataaaag cttcttttat aaaatatata aatctatcga gaaggataga aacttttcca
     5761 ttacttaaaa aaaaagatca taaacatttt tctttttttg gattaatgaa caatagctca
     5821 gtccccatta accggatatt ttcatcaaac caaataagtt agttttctaa aataaattat
     5881 aatagaatct aaaataaaaa aaaataggag gataagtccc taaaataata attggaaatt
     5941 atccgtacat aacccctgct tcttttcgaa tttcttcgtc tctttccaca cccattatct
     6001 tatcaacggc atcgaggaag tcgtttatgt tcacgatggg cctttcttcc cgtatggcga
     6061 acatacctgc ttcggtacag atagccttca catcagcacc tgatgctcca tcggtaaggg
     6121 cagatatcag atcaagatcc acatcctctt ccatgaccat gttttttgtg tgtattttaa
     6181 gaatttctct gcgaccgtct tcattgggta ctggtacttc tatgaaccgg tcaaaacgg
//
LOCUS       DAOQVW010000023         6195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_13997_length_6195_cov_3.618730, whole genome shotgun sequence.
ACCESSION   DAOQVW010000023 DAOQVW010000000
VERSION     DAOQVW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13997_length_6195_cov_3.618730"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /gene="thiC"
                     /locus_tag="PKK85_00940"
     CDS             <1..1241
                     /gene="thiC"
                     /locus_tag="PKK85_00940"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase"
                     /protein_id="HNS24623.1"
                     /translation="TDQIRKVADTEGISSQKLMKRVATGKIVIPCNVNRETSSIGVGK
                     GLSTKINANVGSSPELDDPQQEVEKAILAAEYGAHTVMDLSTGPRFREVRRAIIDAIQ
                     APIGTVPIYQAGIISSKNKGAVVDMDEDDMFSAIEEQAREGVDFMTLHCGITQDTVEK
                     IQRSKRTMGIVSRGGAFLAAWIIHNQQENPLFKNYDYLLEIAREHDVTISLGDGLRPG
                     CLADASDIPQIAELNILGELVQRSRAVNVQVMVEGPGHVPLNQIKTNMQIQKTICKEA
                     PFYVLGPIVTDMAPGYDHITSAIGGAIAASSGADFLCYVTPSEHLSIPNLQEVKDGVI
                     ASRIAAQAADIVLGIDNAWNQEMEMANARKNFNWEKQFELAVDPVKPRKYRERSASPT
                     RDMCTMCGEFCALRIVKDNF"
     gene            1313..1591
                     /locus_tag="PKK85_00945"
     CDS             1313..1591
                     /locus_tag="PKK85_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNS24624.1"
                     /translation="MNFKHVKGENKGKIVLFALSTCGWCNKTRKLIEELGVGYDYVYV
                     DLLEGADRDEAVEALKIWNSNLSFPTLVINDQECIVGFDQDKIMELIG"
     gene            1588..2091
                     /locus_tag="PKK85_00950"
     CDS             1588..2091
                     /locus_tag="PKK85_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNS24625.1"
                     /translation="MISGKSSEEIEKYYHDLKKEVEASGYHFSPDEEFVKELLESILV
                     NKERYGYEACPCRLASGNREEDLDIICPCDYRDLDLGEYDACYCGLYVSERILNGEKK
                     FTCIPERRPGPDERAKIMNKSGGVESNLTYPVWRCKVCGYLCAREEPPEICPICKVGK
                     ERFERFM"
     gene            2207..3019
                     /locus_tag="PKK85_00955"
     CDS             2207..3019
                     /locus_tag="PKK85_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1724 domain-containing protein"
                     /protein_id="HNS24626.1"
                     /translation="MDHMFELYEQVKDDVKFFIASDVRAKIIISLKDGSKNLADLRKD
                     IHLSSSTILHGMSQLEEKNLIFRESGNYSLSQKGEIAAYKLIDLVKSVYSLNKLQSLF
                     LHHDISAIPTNLFKDIGCLENSLIIKATETDIMKPQDVRLAMISKSKNIKHLSSVFDL
                     STTQIFLNALEKGGKVELVLTTGIMEKLEETIDKPALQKWVKKGSLKLMKLDDDVKIS
                     LTIGDDFMTMGLFSTSGAYDLNVALISQEKDALSWGESLYEYHLQKTSKQSL"
     gene            3048..4478
                     /locus_tag="PKK85_00960"
     CDS             3048..4478
                     /locus_tag="PKK85_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS24627.1"
                     /translation="MDDHKKEALKKQKKSDENSLLGFEEKYSALFNSDPTYKIIINLD
                     GKIIDINEQAAQISSSRDEIIGKNFTELKQIFGEGEDPKKYKEILSLVLAGIDVEPVE
                     ITIKDKSGSNKWLELYPSLLEKEGVPVLVQIVAQDVTARKEAEIQLEFQSYILKNVQN
                     PVVVTDLDGKIIYWNAASESTFGYTSEEVEGDSMAIIFPNITEDVLLKILKGQVRRKG
                     SVEYQCQKKAGPLLWMQISINPQLDYKGDVLGYISVFNDITSIKMVEDELKESLSEKE
                     MLLGEINKRLKNYMQMIARLLELQSIYLENVDGSATLHEGQNRLKAMLLIHEGLSKSD
                     DLAIIDFTRFVNKLKKQLMGIYDLENEEVNLKARLDGIMLDVDTAIPCGLIVNELLTQ
                     SFKNAVNGRVLDMNLTLGLDNSGHYKLRYEDTSEGLSGGLITENSDSMIIIRTLVNQI
                     EGDMKISPNHPVVEISWFNPEYSDLS"
     gene            4567..>6195
                     /locus_tag="PKK85_00965"
     CDS             4567..>6195
                     /locus_tag="PKK85_00965"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNS24628.1"
                     /translation="MLYTKLVDIYEALNSTTKRLEKTEILADFLKLIGENEPQILPAV
                     TLMSMGRIFPTWSQKELGIGSKLLMKAIALAVGVTAGEVEDQVRETGDIGMASEKLFY
                     RKSQVTLFTRSLTISKVYNDLVKIADISGGRSQYKKIDILLGLLSSASPLEAKYITRT
                     VIEELRVGVGEGTIKDAISQAFGIPKEVIERSHMLTNDLGLVAAVSRVEGEEGLKKLS
                     LEPGKPVKPMLAQLSPGIEETVKEMGWAICETKYDGIRVQIHRKGEEIEIYTRRLENI
                     SKALPEIVSYIRESLPPRDFIVEGEIIVTRDGKPISFQYILQRVRRKYDIERMVDQIP
                     LTLYLFDTLYYGNNLIDTPFRNRREMLESIVQVNPGKVEISRQVNVTPSDIEKAQELF
                     EYSINEGHEGIMIKDPEAPYMPGIRGKKMLKFKAEPETLDLAVVGGTYGKGKRAHFIG
                     SYLLALQDEDNQLRTIAHVATGLDDKTLAELSQLVEPLITRQKGRKVRIEPKIVLEVA
                     FSEIVKSPEYESGYSLRFPVVKGIRYDLSPEDIDT"
BASE COUNT         2032 a         1117 c         1411 g         1635 t
ORIGIN      
        1 taactgacca aatcagaaaa gttgcagata cagaaggtat aagttcccaa aaattgatga
       61 aaagagttgc aactggaaaa atagtcattc cctgtaatgt taaccgtgaa acctcttcta
      121 taggtgttgg taaaggatta agcaccaaga tcaatgccaa tgtgggttct tctccagaac
      181 tggatgaccc tcagcaggag gtggaaaaag ccatcttggc tgcagaatac ggggctcaca
      241 cagtaatgga tctgagcact ggcccccgat ttagagaagt aagacgagcc ataattgatg
      301 ctattcaagc acccattggc acagtaccca tataccaggc aggtattatc tcttctaaaa
      361 ataaaggggc cgtggttgat atggatgagg atgatatgtt ctcagccatt gaggaacaag
      421 cccgtgaagg tgtggacttc atgactcttc attgcggcat cacccaggac acggtggaaa
      481 agattcaaag gtcaaaaagg accatgggca ttgtaagtcg tggtggagca tttctcgcgg
      541 catggataat tcataatcag caagaaaatc ctctttttaa aaattacgat tatttactgg
      601 agatagcccg ggaacatgat gttaccatat ccctggggga tggattacgc ccgggatgtc
      661 tggctgatgc ctccgacatc ccccaaatag ctgaactgaa tattctggga gaattagttc
      721 agcgatcacg tgctgtgaat gttcaagtaa tggtggaagg tccggggcac gttccattaa
      781 atcaaataaa aaccaacatg cagatccaga aaaccatatg taaagaggcg ccattctatg
      841 ttctgggccc cattgtaacg gatatggctc cgggatacga tcatataact tctgctatag
      901 gtggggctat agcggccagt tccggtgcag attttctatg ttacgtaacc ccctctgaac
      961 acctatccat ccccaatctc caggaagtta aagatggagt aatagcatct aggattgctg
     1021 cacaggctgc tgatattgtt ttaggaattg ataatgcctg gaatcaggaa atggaaatgg
     1081 ctaatgcccg aaaaaacttc aactgggaaa agcagtttga actagcagtt gacccggtga
     1141 aaccgagaaa atatagggaa aggagtgcat cgccaactag ggatatgtgt accatgtgtg
     1201 gagagttttg tgccctgagg atagttaaag ataactttta aaaaattgat cattacaaac
     1261 tattaataat atcattacac taattaataa ataaaaaaat tgggggaaaa gaatgaattt
     1321 taagcatgtt aaaggcgaaa ataaaggtaa aatagtttta tttgctttaa gtacttgtgg
     1381 ctggtgtaat aaaacccgaa aactcatcga agaattaggg gtggggtacg attatgttta
     1441 cgtagatctc ctggagggcg cggatcgtga tgaagcagta gaagctctta aaatctggaa
     1501 ttctaattta tcctttccta ctctggttat caatgaccag gaatgtatcg tgggttttga
     1561 ccaggacaaa ataatggagc taataggatg atttcaggga aaagttcgga agaaatagaa
     1621 aaatattatc atgaccttaa aaaggaggtt gaagcatctg gatatcattt cagcccggat
     1681 gaggaatttg tcaaagaact tctagaaagt attctggtca acaaagagag atatggatac
     1741 gaggcctgcc cttgtcgcct ggcttctggt aacagagaag aggatttgga tataatatgt
     1801 ccctgcgact atcgtgatct agatctgggg gagtatgatg cttgttactg tggtttgtac
     1861 gtttcagaaa gaatactgaa tggtgaaaaa aagtttactt gtattccaga acgacgacca
     1921 ggccctgatg aacgagccaa gattatgaac aaaagtggag gtgttgaatc gaacttaact
     1981 tatcctgtct ggcgttgtaa agtttgtgga tatctatgtg cccgggaaga acctcctgag
     2041 atatgtccca tctgtaaagt ggggaaagaa cgtttcgaaa gattcatgta attatgagca
     2101 taagtgaact ttgtaggggg tgagcaaaaa agcgcaaaac ctataatatt gaactgacaa
     2161 aaactatata gatattagct catagtctgc ggtgattaat gtggatatgg atcacatgtt
     2221 tgaactttac gagcaggtta aggacgatgt gaagttcttt atagcctcgg atgttcgtgc
     2281 aaaaataatt ataagtttga aagatggctc aaaaaatttg gctgatctaa gaaaggacat
     2341 tcatcttagt tcatccacta tactgcatgg tatgagtcaa ctggaagaaa agaatttgat
     2401 ctttcgagag tcaggaaatt attctctctc ccagaagggt gaaatagcag cttataaatt
     2461 gatagatctg gttaaatctg tttattcttt gaataaattg cagagtttat ttttacacca
     2521 tgatatcagt gcaatcccca ccaacctttt caaggatata ggttgtttgg agaattcgct
     2581 aataatcaag gccactgaaa ctgatatcat gaagccccag gacgttaggc tggccatgat
     2641 atctaaaagt aagaatatta aacatctatc ttcggttttt gatctttcca ccacccagat
     2701 atttttgaac gccttggaaa agggcggaaa ggttgaactg gttctaacca cgggcataat
     2761 ggaaaaatta gaagaaacta tagataaacc ggcccttcag aagtgggtaa aaaaaggcag
     2821 tctcaaattg atgaaacttg atgatgacgt caagatatct ttaacaattg gagacgattt
     2881 catgaccatg ggtctatttt caaccagcgg agcctatgat ctcaatgtag cccttataag
     2941 tcaagaaaag gatgcattgt cctggggaga atctctatac gagtaccacc tccaaaaaac
     3001 ctcaaaacaa tcgttataag aactaatatt attttcaggt ggtttatatg gacgaccaca
     3061 agaaagaagc cctaaaaaaa cagaaaaaat ctgatgaaaa ttcattactg ggatttgaag
     3121 aaaaatattc agcattattt aactctgatc ctacttataa aattataatt aacttggatg
     3181 gaaaaattat cgacatcaat gaacaagctg cccaaatcag ttcttcccgt gacgaaatta
     3241 ttggcaaaaa tttcactgaa ctgaaacaga tttttggtga aggagaggat cctaaaaaat
     3301 acaaggagat attgtcatta gtcctggcag gcattgatgt agaaccagtt gaaataacaa
     3361 ttaaagataa aagtggttcc aacaagtggc tggagctata tccttcccta cttgaaaaag
     3421 aaggggtacc ggttctggtg cagatcgtgg cccaggacgt gactgcccga aaggaagcag
     3481 aaatacaact ggaattccag agttatattt tgaaaaatgt ccagaaccca gtagtggtga
     3541 ctgatctgga tggaaaaata atatactgga acgcggcttc cgaatcaacc tttggttata
     3601 cttcagaaga ggtagaaggg gatagtatgg ctataatttt ccccaatata actgaagatg
     3661 tgcttttaaa aattttaaag ggccaggtaa gaaggaaggg aagtgttgag taccagtgcc
     3721 agaaaaaagc aggcccactg ctgtggatgc agatcagcat caatccacaa ttagactaca
     3781 aaggtgatgt tttagggtat ataagtgttt ttaatgatat aacctctatt aaaatggttg
     3841 aagatgaatt aaaagaatca ttaagcgaaa aagaaatgtt gttgggtgag ataaataaac
     3901 ggcttaagaa ttacatgcag atgatagctc gtcttctgga attacaatcc atctacttag
     3961 aaaatgtaga tggtagtgca actcttcatg aaggccaaaa caggctaaaa gccatgttat
     4021 taattcatga aggtctttcc aagtctgatg atctggccat aatagacttc acccgattcg
     4081 ttaataagct taaaaaacag ttaatgggca tttatgactt ggaaaatgaa gaggtaaatc
     4141 taaaagcccg gttagatggc ataatgctcg acgttgacac tgccatacca tgtggcctca
     4201 ttgtcaatga acttctgacg caatcattta agaatgctgt taatggaagg gtcctggata
     4261 tgaatctgac tttaggacta gacaactcgg gacattacaa attaagatat gaagatactt
     4321 cggaaggtct ttcaggagga ttaatcaccg aaaatagcga tagcatgatt attatacgga
     4381 ctctggtgaa tcagattgag ggtgatatga agatatctcc taaccaccca gtggtggaaa
     4441 tttcctggtt caatccagaa tattcggatc tttcatagtc caccaccttc tttttaatga
     4501 taaatctgga cagcccattc agaacacacc atttcattgt ggaggggatg cctattgacg
     4561 gataaaatgc tttatacgaa gttggtggat atctacgagg cccttaactc caccaccaag
     4621 cgtctggaaa agacggaaat actagctgat tttctaaaat tgataggaga aaacgaacca
     4681 caaatcctac cagcggtgac tttgatgtcc atgggccgga tatttcctac ctggagtcag
     4741 aaagaactgg gaataggatc taaactccta atgaaagcca ttgccttagc agtaggggtg
     4801 actgccggtg aggtagaaga ccaagttcgt gaaaccgggg atataggtat ggcttcagaa
     4861 aagctttttt atagaaaaag tcaggtcacc ctgttcaccc gttccctgac catttcaaag
     4921 gtgtacaatg atttggtgaa aatagcagat atctccggtg gaagatctca gtataaaaag
     4981 attgatatcc tactaggatt gctatcatcg gcatcccctt tggaagccaa atacataact
     5041 aggactgtga ttgaggaatt acgagtaggg gtgggggagg gaactattaa agatgctata
     5101 tcacaagctt tcggtattcc taaagaagtc attgaaagat cgcacatgtt aaccaatgac
     5161 ctgggtctgg tggccgcagt ttcccgggta gaaggagaag aaggtctgaa aaaactttca
     5221 ttagaaccag gtaaaccagt taaacccatg ttagcccaat tatctcctgg aattgaggaa
     5281 actgtgaaag aaatgggatg ggccatctgc gaaactaaat acgatggtat ccgggtgcag
     5341 atacatcgta aaggtgaaga aattgagatc tatacccgta gattagaaaa catctccaaa
     5401 gcattaccag aaattgtttc ttacatccgt gaatcacttc ccccaaggga cttcattgtg
     5461 gaaggtgaga taattgtcac ccgcgatggg aaacccattt ccttccagta catattacag
     5521 agggtgcgtc gaaagtacga tatcgaacgc atggtagatc agattccact caccctttac
     5581 ctgttcgata cactttacta tgggaacaac cttattgata ctccgttccg taataggaga
     5641 gaaatgttgg agtctattgt ccaagtaaat cctggaaagg ttgaaatttc tagacaagtc
     5701 aacgtcacac cttcggatat agaaaaagcc caggaactct ttgaatactc cattaatgag
     5761 gggcatgagg ggatcatgat taaggatccc gaagcaccct atatgccagg tattcggggt
     5821 aagaaaatgc tcaagttcaa ggccgaacca gaaactctgg acctggcggt agtgggaggg
     5881 acttatggga agggtaaaag ggcccacttt attggttcct acctattagc cctccaggac
     5941 gaggacaacc aactcagaac tatagcccat gtggccacgg gccttgatga caagacattg
     6001 gcagaactct cacaattggt ggaaccactc attactcgtc aaaaaggtcg aaaagttaga
     6061 attgagccta aaattgtatt ggaagtagct ttcagcgaaa tcgtgaaaag tccagaatac
     6121 gaaagtggat attccctccg tttcccggtg gttaagggca tacgttatga tctcagcccg
     6181 gaggatattg acacc
//
LOCUS       DAOQVW010000024         6131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_14200_length_6131_cov_3.574226, whole genome shotgun sequence.
ACCESSION   DAOQVW010000024 DAOQVW010000000
VERSION     DAOQVW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14200_length_6131_cov_3.574226"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..314
                     /locus_tag="PKK85_00970"
     CDS             57..314
                     /locus_tag="PKK85_00970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HNS24629.1"
                     /translation="MKIIKDEKKELEIEITGESHSLCNALRTVLMEDKNVEAAAYAID
                     HPIIGQPRLYIKAKNPRQSLKKAVKTLQKRCEEVKELVKPV"
     gene            317..925
                     /locus_tag="PKK85_00975"
     CDS             317..925
                     /locus_tag="PKK85_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HNS24630.1"
                     /translation="MNEEIPNKKFRKIKKEIRILGVDDGPFTPHREEKVLIIGTIFRA
                     GIWLDGVLSTQITNDGDDATDKLVGMVNQSHHREQIGVIMLDGITFGGFNVVDIQQIF
                     QKTQIPVIVIMRKQPDLLKIRNALQNFSNWEERWKRIQKAGKIKIIRNKELIYIQTSG
                     ISWEDAEEIVKLATTRSAIPEPIRAAHIIASGVITGDSRGSA"
     gene            991..1392
                     /locus_tag="PKK85_00980"
     CDS             991..1392
                     /locus_tag="PKK85_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNS24631.1"
                     /translation="MKQPSLTVDIVIVHDDGTVMLIKRKNNPFKGLWALPGGFVEYGE
                     TVEQAAVREAREETGIFVDIKKLVGVYSSPDRDPRGHTVTICVLAKPSGGTVTPSTDA
                     ADASYFSLYEINSLKLAFDHKKILKDALKTL"
     gene            1441..1755
                     /locus_tag="PKK85_00985"
     CDS             1441..1755
                     /locus_tag="PKK85_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HNS24632.1"
                     /translation="MEFCPKCGTVMFPKGDCFECKCGYQKKITKKSLSDYEVTEKISS
                     RENVIVTGDDIKTLPTTKAVCPKCQNKEAFWWMRQTRGADEAETRFFKCTKCPHTWRE
                     YD"
     gene            1766..2362
                     /locus_tag="PKK85_00990"
     CDS             1766..2362
                     /locus_tag="PKK85_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24633.1"
                     /translation="MKDDVRSDTTPKSSENQVKEKGSRSETSTNHEFERHKKFTADSH
                     MDKGDVVSESPNSMKKTRNETGKEAEDHEFPELNFKEVVRDMEVTKYIATEKTRIIRY
                     ASAIIGGMFLLSGFILLYTAATRVVDNVIFGERAMFSSFLILIGMLILITVFGRRYWH
                     DTVLTKLNVGMEISEGKSKSKDKDKITKKDNIEEENKK"
     gene            2379..3113
                     /gene="pcn"
                     /locus_tag="PKK85_00995"
     CDS             2379..3113
                     /gene="pcn"
                     /locus_tag="PKK85_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030337 - DNA polymerase processivity
                     factor activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proliferating cell nuclear antigen (pcna)"
                     /protein_id="HNS24634.1"
                     /translation="MFKAVLSDSNILKTSFDAISSIVDEVQMQADEEGLRLDALDRSH
                     ITFVHLELKKNVFDEYQCKEPLKINVDTEELMKVLKRAKSEDMVELTTDEGNLIITFE
                     GEARRRFKIRLIDIEYEAPSPPQLDYPTEFEIPFSLLKDSIQDIGIVSDKIALQVDED
                     KFKASAEGDFGDAKIEYLHGEKIQENAKSVFSLEKVKEMMKADKFSETAQIKLGNDMP
                     LNLYLKMASDEGELSFLLAPRIESEE"
     gene            3116..4024
                     /locus_tag="PKK85_01000"
     CDS             3116..4024
                     /locus_tag="PKK85_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24635.1"
                     /translation="MDEFFQRLREIQKKERGLSGLSPVGDDFYLRVFRYLDGLMEKIG
                     NNQLSLDSYLLLRDAQRIVAEICERREHKITNSAVMNVQRSFQLFIEQKDQISPQIPI
                     NSTPEEIDLYNELFKSLSQHRKAMNFSTSSSPVIEKKKKDSGTGTDDAAGSNQEIPKK
                     TFSSEKSSTRLVDEFDENSISPPIEDEIYLQFGNEPTLKNVNTAVSVKKTVKPDETVQ
                     IKENRVKRRKIPRVSSSTGFSDKGKMLPEMSTAVLVITAELPSIMGADHKVYGPMYPG
                     DVITMPEANARILIKKDKGRPIQRYK"
     gene            4139..4417
                     /locus_tag="PKK85_01005"
     CDS             4139..4417
                     /locus_tag="PKK85_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HNS24636.1"
                     /translation="MKIPKERRTYCPSCKKHTVHEVFESKRRKASELKWGQRQFRRVT
                     SGYRGYPRPLPSGNKPTKKLDLRYKCKSCGKSHIKRSTFRTGKVEFTM"
     gene            4427..4603
                     /locus_tag="PKK85_01010"
     CDS             4427..4603
                     /locus_tag="PKK85_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HNS24637.1"
                     /translation="MSKRKSNFLRVKCGDCGNQQVVFDHASSKVECIICSKALVQPKG
                     GKSKIVAQIIEVID"
     gene            4613..5392
                     /locus_tag="PKK85_01015"
     CDS             4613..5392
                     /locus_tag="PKK85_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HNS24638.1"
                     /translation="MVRTKNEWPAERDLVVGTVHKVLNYGAFAYLDEYPGKEAFIHIS
                     EVSAGWVKNIRDYVRENQKIVARVLRVNPRKGHVDVSLKRIREDQRTRKIQQWKIEQK
                     AEKLLEFTAKSLGKDLDAGYDEVGYAIMDEFGDLYGAFEAAAEEGVDVLVERGVDENW
                     ARAITEVAEKNISPPEVQINGYVNLTSYAPNGVETIRNALGSIKDENISVQCVGAPKY
                     RLTVVSSDYPTAEDILNKTAQKAIKFVEKAGGTGEFHRELE"
     gene            5389..5574
                     /locus_tag="PKK85_01020"
     CDS             5389..5574
                     /locus_tag="PKK85_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HNS24639.1"
                     /translation="MKSKMKCCISCSEYTIQDRCPYCGGNVRVIFPPKYSPEDKYGKY
                     RRILKEQLRSNLNNNSD"
     gene            5616..>6131
                     /locus_tag="PKK85_01025"
     CDS             5616..>6131
                     /locus_tag="PKK85_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HNS24640.1"
                     /translation="MNKTIISLFKDVDLDEPIFIEALPGIGHVGKLVAEHLIHELEAE
                     KLADLFSPSFPPQVFVGENGIIEPMKNEFYYLKSAGEDERDYIILVGNTQGLSPEGQY
                     EICGAIIDFVKKYGAKQMFTLGGLGTGQPVDKPKVYGAATNIELAENLKEYGVTLRSA
                     DGGIIGASGLLL"
BASE COUNT         2136 a         1079 c         1385 g         1531 t
ORIGIN      
        1 ctaactgtgg taataaagag actcggaagt tatcttctaa atatgaggga tgaatcatga
       61 agatcataaa ggatgaaaag aaggagctgg aaattgaaat tacaggcgaa tctcatagtc
      121 tctgcaacgc ccttcgaacg gttttaatgg aagataaaaa tgtggaagca gcggcttacg
      181 ctattgacca ccccataatt ggacagccaa gactgtatat aaaggctaaa aatccccgtc
      241 agtcacttaa aaaggcagtt aaaactttac agaaacgttg tgaggaagtt aaggaactgg
      301 taaaacctgt ttaaggatga acgaagaaat ccccaataaa aaattccgga agataaagaa
      361 ggaaatacgt atactgggag tcgatgatgg acccttcaca ccacaccgag aagaaaaagt
      421 cctaattatt ggaaccatat tcagagcagg aatttggctg gatggcgttc tgagtaccca
      481 aatcaccaac gacggtgatg atgcaactga caagttagtg ggaatggtga atcaatccca
      541 ccaccgtgaa cagatagggg tgatcatgct ggatggaatt acctttggtg gttttaacgt
      601 tgtagatatc cagcaaatat tccagaaaac acagatcccc gtaattgtta tcatgcgtaa
      661 acagccagat ttactgaaaa taagaaatgc tctccagaat ttttccaact gggaagaaag
      721 atggaaacgt atccaaaagg caggaaaaat aaagataatc cgcaataaag aactaattta
      781 cattcaaacc agcggaatat cctgggaaga tgcagaagaa atagtgaaac tggcaactac
      841 cagaagcgcc ataccggagc ccatccgcgc cgcccatata attgcctccg gggttataac
      901 tggagattcc cggggaagtg cataaattgt ggtaaaaact tcgtaaaaga gctaatagta
      961 aaattattat tctaatcagg aagtgtttaa attaaacaac cctctttaac cgttgacata
     1021 gtaatagtcc atgatgatgg gacagtaatg cttataaaaa gaaaaaataa tccatttaaa
     1081 ggattatggg cattaccggg ggggttcgtt gagtacgggg agactgtgga gcaagcggca
     1141 gtacgggaag ctagagaaga aacaggaata tttgtggata taaaaaagct ggtaggtgtt
     1201 tattccagcc cggatcgtga tccacgggga cacactgtta ccatttgtgt cctggctaaa
     1261 ccctcaggag gaacagtcac gccctccaca gatgcggctg atgcatcata cttctcttta
     1321 tatgagataa attcccttaa actagccttt gatcataaaa aaattttaaa agatgcttta
     1381 aagactttat aatactgtaa attaataaat ccaataatac agataatcag gaggaccaaa
     1441 atggagttct gtccaaaatg tggaacagta atgtttccga aaggggactg ctttgagtgt
     1501 aaatgtggat atcaaaagaa aataactaag aaatccctga gcgattatga agttacagag
     1561 aaaatatctt ccagggagaa tgtgatagtc acgggtgacg acattaaaac tcttcccaca
     1621 accaaggccg tgtgcccgaa atgccagaac aaagaggctt tctggtggat gcgccagacc
     1681 cgtggggcag acgaagctga aacacgattt tttaagtgca caaaatgtcc tcacacttgg
     1741 agggaatacg actaaggaga atgttatgaa ggacgatgtt agatctgata ctaccccaaa
     1801 atcttcagaa aatcaggtaa aggaaaaagg cagtaggtca gaaaccagca ctaatcacga
     1861 attcgaaaga cataaaaaat ttacagcaga ttcccacatg gacaagggcg atgttgtatc
     1921 agaaagtccg aattccatga aaaaaacaag aaatgaaaca ggcaaagaag ccgaagatca
     1981 tgaattcccc gaattaaact tcaaagaagt agttagggac atggaagtga ccaaatatat
     2041 cgcaaccgag aaaacgcgca ttataaggta tgcttcagct attattggtg gaatgttcct
     2101 tttatccggt ttcatactcc tatatacagc ggctacacgg gtggttgata atgtaatatt
     2161 cggggaacgc gccatgtttt cttcattttt gattttgatt ggtatgttga ttctcataac
     2221 tgtatttggc cgccgctact ggcatgacac tgtcctcact aaactaaacg tgggaatgga
     2281 aatttctgaa ggaaaaagta aatcaaaaga caaggacaaa ataacaaaaa aagataatat
     2341 tgaagaagag aataaaaagt aattaaagga ggaacaacat gttcaaggca gttttaagcg
     2401 attcaaacat tttaaaaacc agtttcgacg caatatcgtc cattgtggat gaggtacaaa
     2461 tgcaagccga tgaagaaggt cttagactgg atgctctgga ccgcagccac atcacattcg
     2521 tacatctgga attgaagaag aatgtttttg atgaatatca atgtaaagaa cctctcaaaa
     2581 tcaatgtaga tactgaagaa ttgatgaaag tccttaaacg agccaaatca gaggatatgg
     2641 tggagctgac cactgatgag ggtaacttga tcataacctt tgagggtgaa gcacgccgta
     2701 gattcaagat caggctaatt gacattgaat atgaggcacc tagccctcct caacttgatt
     2761 accccaccga atttgaaata cccttcagtc tcctcaagga ctctatacag gatattggaa
     2821 tagtttctga caaaatcgct ctccaagtag atgaagataa gttcaaagct tctgcagaag
     2881 gagactttgg ggatgccaag atagaatact tacatggtga aaaaatacag gaaaatgcta
     2941 aatcagtttt cagtctagag aaagttaagg agatgatgaa ggctgataaa ttctcagaaa
     3001 cagcacaaat taagttggga aatgacatgc cccttaacct ttatttaaaa atggcttcag
     3061 atgaaggtga attaagcttt cttttagctc caaggataga aagtgaggaa tagggttgga
     3121 tgaatttttc cagaggctaa gggagatcca gaaaaaggaa cgcggcctga gtggattatc
     3181 tccagtgggg gatgacttct acctaagagt ttttcgttat ctagacggcc taatggaaaa
     3241 aatcggaaat aatcaactgt cattggattc ttatttacta ctccgggatg ctcaacgaat
     3301 tgttgctgag atctgtgaaa gaagggaaca taaaattact aacagtgctg taatgaatgt
     3361 tcaacgttct tttcagctat ttatagaaca aaaggatcaa atcagccccc aaattcctat
     3421 taactctact ccggaagaaa ttgatttata caacgaatta ttcaagtctt tatctcaaca
     3481 tcggaaggcc atgaattttt ctacaagttc atctccagta atagaaaaga aaaaaaagga
     3541 tagtggaact ggtactgatg atgcagctgg atctaaccag gaaattccta aaaaaacttt
     3601 ttcatcggaa aaatcttcaa ctagattggt agatgaattc gatgaaaatt ctatatcccc
     3661 tcccattgaa gacgaaattt acctccaatt tggcaatgaa ccaaccctaa aaaatgtgaa
     3721 cacggctgtg tcggtaaaaa aaacagtcaa accagatgaa acagtccaaa ttaaagaaaa
     3781 cagggttaaa agaaggaaga ttccccgggt atcttcgtcc actggatttt cagacaaggg
     3841 aaagatgctt cctgagatgt caaccgcggt tttagtgata actgctgaac ttccctctat
     3901 catgggggcg gatcacaagg tttacggtcc catgtatcct ggagatgtca ttaccatgcc
     3961 cgaagccaat gcccggatac ttatcaagaa agataaggga aggccgatac aaaggtataa
     4021 ataaagattg aaagatactt aatttgaata aaaaacaggc ttcttaaaat tgttcaaggc
     4081 ccctgaagtg atttttacca gtcacaaggc ttattttaca ttcaagaggt gatttaatat
     4141 gaaaattcct aaagaaagga gaacttactg tccaagttgt aaaaaacata ctgttcacga
     4201 ggtattcgag tcaaagagga gaaaagccag cgaactcaaa tgggggcagc gccaattcag
     4261 aagggtgacc agtggttaca ggggataccc taggcctctc ccatcaggaa ataaaccaac
     4321 taaaaagttg gatttacgtt ataagtgtaa aagttgtgga aaatctcaca taaaacgatc
     4381 cacattccga accggaaaag tagagtttac gatgtagggt gatgtgatgt ctaaaagaaa
     4441 gagcaatttt ttaagggtca aatgtggaga ctgcggaaac cagcaagtgg tattcgatca
     4501 tgcttcatcc aaagtggagt gcattatatg cagtaaggca ctggtacagc ctaagggtgg
     4561 aaaatccaaa attgtagccc aaattataga agtaattgat taaggtgact tgatggtaag
     4621 aaccaaaaac gaatggcctg ctgagaggga tttagtagtg gggacagtgc acaaagtcct
     4681 taactatggt gcatttgctt atctagatga atatcctggt aaagaagcat tcatacatat
     4741 ttctgaagtt tccgctggtt gggttaaaaa tattagagac tacgtccggg agaaccagaa
     4801 gattgtggcg cgtgttcttc gagttaatcc tcgtaaagga catgtagatg tttctcttaa
     4861 aaggatccgt gaagatcaga ggactcgtaa gattcagcag tggaaaattg aacaaaaggc
     4921 cgaaaaactt cttgaattca cagctaagag tttggggaag gatctggacg cgggttatga
     4981 tgaagttgga tatgccatca tggatgaatt tggggatttg tacggggcct tcgaagctgc
     5041 tgctgaagag ggagttgatg tactggtgga gcgaggagtg gatgaaaact gggccagagc
     5101 cataaccgaa gtggctgaga aaaacatatc acccccagaa gtgcaaatca atggatacgt
     5161 taacctgacc tcttatgcgc ccaatggggt ggaaacaata aggaacgctc tgggatccat
     5221 taaagatgaa aatatttctg ttcaatgtgt aggagccccg aaataccgtt taacagtagt
     5281 atcttctgat tatcctactg ctgaggatat attgaataaa actgctcaaa aagccattaa
     5341 attcgtggaa aaagccggag gcacgggcga atttcacaga gaattagaat gaagtccaag
     5401 atgaagtgct gcatttcctg cagtgagtac accattcagg atcgttgtcc atattgtgga
     5461 gggaatgtga gagtcatctt tccaccgaaa tattcgccgg aagataaata cggtaaatac
     5521 agaagaatct tgaaggaaca acttaggagc aatctaaata acaattctga ttaaattaca
     5581 tattaaaatt gttccccaat aaagggagtg aattaatgaa taaaaccatt ataagcctgt
     5641 ttaaagatgt tgatctggat gaacccatat ttatagaggc tctgcctgga attggacatg
     5701 tgggaaagtt ggtggcagag catctgatcc atgaactgga agccgagaaa ttagccgatc
     5761 ttttctcacc ctctttccct ccacaagtct ttgtgggtga aaacggaatc atcgaaccaa
     5821 tgaaaaacga gttttactat cttaaatctg ctggagagga tgaaagagac tacataatac
     5881 tggtaggaaa cactcaagga ttgagtcctg aaggacaata cgaaatctgt ggtgcaataa
     5941 ttgattttgt caaaaaatac ggtgcaaaac agatgtttac tttaggtggc ttgggcactg
     6001 ggcagcctgt agacaaaccc aaagtatatg gggcggctac taatattgaa ctggcagaaa
     6061 acctcaaaga atatggtgtg actttaagat ccgccgatgg ggggatcata ggtgcgtctg
     6121 gtttattact g
//
LOCUS       DAOQVW010000025         6071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_14375_length_6071_cov_2.876496, whole genome shotgun sequence.
ACCESSION   DAOQVW010000025 DAOQVW010000000
VERSION     DAOQVW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14375_length_6071_cov_2.876496"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /gene="acsC"
                     /locus_tag="PKK85_01030"
     CDS             complement(<1..1207)
                     /gene="acsC"
                     /locus_tag="PKK85_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HNS24641.1"
                     /translation="MQLTAMDIYKLLPKTNCEDCGEASCMAFATKLSEKETQLDLCTE
                     LESEAFTELEAMLAPAVREITIGTGDKTVTIGGDEVLYRYELTYYNPTALVIDIADNL
                     SSDEFQERVKTIQGTEFERIGEILKLDAIALRNASGDPAKFAEAATKLKDTRFPLVLC
                     SFDPEAMRAALEKVGDEKPLIYAVNESNLEEMAALALEYHCPLTIFSPNDLEKMKNIS
                     RALRSKGIEDIVLDPGTFVEAGVGDSLDDFVMIRRLAVDFRFPILGIPALTWLYEKDE
                     VQGGIREATIAATLMNKYADVLIFHGTSIWELIPVLTLRQGIYTDPRKPQAVDAGLYE
                     FGDVDQDSPVLMTTNFALTFYTVEGDIKGKANTMNLQAKQRATTVAGIRRDEVVIKSQ
                     SELYWDMDPL"
     gene            complement(1220..2380)
                     /gene="cdhD"
                     /locus_tag="PKK85_01035"
     CDS             complement(1220..2380)
                     /gene="cdhD"
                     /locus_tag="PKK85_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase subunit
                     delta"
                     /protein_id="HNS24642.1"
                     /translation="MDKISQLLKLLEKTDYLEINEFRMDFDELELQLAPAMQRIMQQA
                     VQQQAVVKQASEIESLEFTPPVQDYPGEVAEVQLGAGSRKPVFLGGQKALYRFEDPQP
                     NPPVVTFDVFDIPMPGLPRPIREHFSDVMDHPGDWAKKAVKEFGANMVTIHLIGTGPK
                     VMDKTPREAAQDIEEVLQAVDVPLVVGGSGDPQKDPVILEAAAAAAEGERCLLASANL
                     DLDYRMVAKAAVDYNHAVLSWAITDVNMQKTLNKYLMKEGLTPNDIVMDPTTCALGYG
                     IEFSIDVITRTRLAALKGDQDLQMPMSSGTTNAWGSREAWMKNDDWGPTDYRGPIWEI
                     VTGLTMMLCGVDIFMMLHPLSVQLLSEIGSTFTKEYLSTDVPDISNWITELE"
     gene            complement(2392..3147)
                     /locus_tag="PKK85_01040"
     CDS             complement(2392..3147)
                     /locus_tag="PKK85_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS24643.1"
                     /translation="MIIAISGKGGVGKTLISSLLIKELSETGKDILAIDADPDSNLPE
                     ALGVDVDRTVGDIREELKNDTAQGKIPTGMNKWDILDYKIMESIVETPEYDLLVMGRP
                     EGSGCYCAVNNMLRRIIENLSENYDFIIIDTEAGLEHLSRRTTQNVDVMLVVTDNSRR
                     GMLTAHRIGDLAGELEIKFQDLYLVLNRVRPEVEEEIVKKAQETGLEIRGIVHEDDKV
                     TEYDLEGKPLVELPDDSKTLMAVSGILSRILRE"
     gene            complement(3184..4581)
                     /gene="cdhC"
                     /locus_tag="PKK85_01045"
     CDS             complement(3184..4581)
                     /gene="cdhC"
                     /locus_tag="PKK85_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_function: GO:0043884 - CO-methylating acetyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HNS24644.1"
                     /translation="MFEDIPVDVSPMYEGERIRSANMFVELAGPKSWGAELVQVEETV
                     EDGKVEVIGPELQDMGEGDIHPLGIKIEIQGEALEKELEGVIERRTHELCNYIKGFMH
                     LNQRDQIWCRVSKEALEAGFKLEDLAKALLILFKEEYPIIESIAITIMTDEDEVQRFV
                     EKARLQYEQRDARARELSDEDVDVFYGCVMCQSFAPTHVCVVTPDRTALCGAINWFDC
                     RAAAKMDPDGPIFEIEKGEVLDEVKGEYSTVNEVVSQRSQGTFDTVYLHSVFGHPHTS
                     CGCFEAVAFYIPELDGIGIVDRDFRGETPLGIPFSAMAGQCSGGKQVEGFTGLSLEYM
                     RSPKFLQADGGYERIIWLPKEIKESLSEYIPGDLKDKIPTEEESANIKDMRAFLEEKG
                     HPILDRLEGTPGEVEEAPVQEEESVEYAEMDMEAMPVAPVAYAPEFTMPASGGVKIIF
                     KNAKIYAEKVIIKKN"
     gene            complement(4622..5137)
                     /gene="cdhB"
                     /locus_tag="PKK85_01050"
     CDS             complement(4622..5137)
                     /gene="cdhB"
                     /locus_tag="PKK85_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase complex
                     subunit epsilon"
                     /protein_id="HNS24645.1"
                     /translation="MSNDRVIPWQPTVIAGPKQALLVTPETAELMIRKSKRPLLVLGP
                     LVKEEPLLSLAVGIAEKWDLPVVTTGDAYKAFREKDLDSVAYGAIEIVNLLKDPEWVG
                     VNGEGQHDLVIFLGCIYYLASQGLSALKHYAPHLKTLTICKFFHSNADASFPNMKDEE
                     WLKYLAKMAGQ"
     gene            complement(5139..>6071)
                     /locus_tag="PKK85_01055"
     CDS             complement(5139..>6071)
                     /locus_tag="PKK85_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha"
                     /protein_id="HNS24646.1"
                     /translation="EHFVKDEKFSIRAGRGPVQDVEIRRVGAPLVLGDIPGVIAFVGC
                     SNYPDGGKEVAEMAKEFLERNYIVVATGCGAMSIGEYKDEEGKTLYEQYSGDFDARGL
                     VNMGSCVSNAHISGACIKIANIFAKKPLEGNFEEIADYILNRVGACGVAWGAYSQKAA
                     AIATGVNRWGIPVVIGPHGSKYRRLYLGRADKKDNWKIKDLRTGKIMDGEPAPEHLIY
                     AAETVEESMPMIAKLCIRPNDTAKGRQIKLNHYIDLYKRYFGVLPPDINLFVRNDKDI
                     PITHKKEVKDLLEEVGWEPREIPQEPSLMGLDGD"
BASE COUNT         1395 a         1584 c         1289 g         1803 t
ORIGIN      
        1 gaagtggatc catatcccag taaagctcac tctggctttt gattaccact tcgtcacggc
       61 gaataccggc cacggtggta gcacgctgtt tggcttgcag attcatggta ttggctttgc
      121 ccttgatatc cccttccacg gtgtagaagg tgagggcgaa gttggtggtc atcaggactg
      181 gtgaatcctg atccacatcc ccaaactcat aaagtccggc atccactgcc tggggctttc
      241 tggggtcagt gtagataccc tgtcttagag tcaggactgg aattagttcc catatactgg
      301 ttccatggaa tatcagaaca tctgcatact tgttcattaa ggtagcagca atggtagcct
      361 ccctaatacc tccctggact tcatcctttt cgtacaacca ggtgagggcg ggtatgccca
      421 gtatggggaa tcggaagtcc actgccaacc tccggatcat aacaaaatca tccagactat
      481 cccctacacc agcttccaca aaggttcctg gatccaggac tatatcctct attcccttac
      541 tcctcagagc ccgactgatg ttcttcatct tctccaggtc gttagggctg aagatggtca
      601 ggggacaatg gtactccagg gctaatgccg ccatttcttc caggttagac tcgttaactg
      661 cgtagataag gggtttttca tccccaactt tttccagggc cgctctcata gcctccgggt
      721 cgaatgaaca tagaaccagg gggaacctgg tatctttgag tttggttgct gcttcggcga
      781 atttcgccgg gtctccagag gcattccgga gtgctatggc atcaagtttg agtatttcac
      841 cgatcctctc aaactcggtg ccctggatgg ttttaaccct ttcctggaat tcatcagaag
      901 acaggttatc ggcgatgtca attaccaggg cagtgggatt gtagtaggtt aactcataac
      961 ggtacagaac ctcgtctcca cctatggtta ccgtcttgtc acctgtacca atggtgatct
     1021 cccttactgc gggtgcaagc atggcttcca gttctgtgaa tgcctctgac tccagttcag
     1081 tgcacagatc cagttgtgtc tctttttcgg atagtttagt ggcgaaggcc atgcaggagg
     1141 cctctccaca atcttcacaa ttggtcttgg ggagtaattt gtaaatatcc atggctgtaa
     1201 gttgcatact cataacctcc tattccaact ccgttatcca gtttgatatg tcaggtacat
     1261 cagtgctgag gtactccttg gtgaatgtgg agcctatttc gcttaatagc tgcactgata
     1321 gggggtgcag catcatgaat atatccactc cacagagcat catggtcaaa ccagtgacga
     1381 tctcccagat ggggccccgg tagtcggtgg gtccccagtc atcgtttttc atccaggctt
     1441 ccctggatcc ccaggcgttg gtggttccag agctcatagg catctgcagg tcctggtctc
     1501 ctttcagggc agccagtctg gtccgggtga tgacgtcaat ggaaaattcg ataccatatc
     1561 ccagggcaca ggtagtgggg tccatgacta tatcgttggg ggtgagtccc tccttcatca
     1621 ggtacttgtt gagggtcttt tgcatgttca catctgtgat ggcccagctt aaaactgcgt
     1681 gattataatc aactgcagct ttggccacca tccggtagtc cagatccagg ttagccgagg
     1741 ctaaaaggca tctttctcct tcagctgcag ctgcagctgc ctccaggatc accgggtctt
     1801 tctgtggatc accggaacca cccaccacca ggggcacgtc caccgcttgc agtacttcct
     1861 ctatgtcctg agccgcttca cggggtgttt tgtccataac cttgggacca gtgccaatta
     1921 ggtgaatggt gaccatgtta gctccaaatt ccttcaccgc ctttttcgcc cagtccccag
     1981 ggtgatccat aacatcagag aagtgttccc ttatgggcct aggcagtcca ggcatgggga
     2041 tatcgaatac atcgaaggta accactggcg gattgggctg gggatcctca aaacggtata
     2101 gggctttttg gccacctaaa aatactggtt tgcggcttcc ggcccccagt tgtacctctg
     2161 ctacctctcc tgggtagtcc tgtactggtg gtgtaaattc cagtgattca atctccgaag
     2221 cttgcttcac cacggcttgt tgttgtacag cttgctgcat tattcgctgc atggcagggg
     2281 ccagttgcag ttctaattca tcaaaatcca tacgaaattc atttatttct aaataatctg
     2341 ttttttccaa tagtttaaga agttgcgata ttttatccat atacccacac cctattctct
     2401 taaaattcgg gacaaaatcc cggatactgc catcaacgtc ttagaatcgt ctggtaattc
     2461 caccagaggt tttccctcca aatcatattc cgtgacctta tcatcttcat gtacaattcc
     2521 ccggatttca aggccagttt cctgggcttt cttgacaatc tcttcttcaa cttcaggccg
     2581 aaccctattt aagactaggt acagatcctg gaatttgatt tccagttcac cagccagatc
     2641 cccaattcga tgggcagtta acatccccct tctggagttg tcagtaacaa ccagcattac
     2701 atccacattc tgggtggttc tgcggcttaa atgttccagt ccggcttcag tgtcaattat
     2761 tatgaagtca tagttttcgg agagattctc aataatcctg cggagcatgt tgttaactgc
     2821 acagtaacag ccactccctt caggacgccc catgaccaga agatcatatt ccggagtttc
     2881 aacaatggac tccataatct tgtagtccag tatatcccat ttgttcatcc cggtgggaat
     2941 tttaccctgg gctgtgtcat ttttgagttc ttcccggata tctcccacgg tacgatccac
     3001 atcaactccc agggcttcag gaaggttgga atccgggtca gcatctatgg ccagaatatc
     3061 cttcccagtt tcagatagct ccttaataag aagtgatgag atcagggtct taccaactcc
     3121 acctttgcca ctgattgcaa taatcaaatt gatcctcctt gatgaaatac catttttaga
     3181 tgtttaattt tttttaataa tcaccttctc ggcgtagatt tttgcattct taaagatgat
     3241 tttcacccca cccgatgcag gcatggtaaa ttccggggcg taggcaactg gagctactgg
     3301 cattgcttcc atgtccattt cagcatattc aacagactct tcctcctgga caggggcttc
     3361 ttccacttcc cctggagtgc cttccaatct atccaggata ggatgcccct tttcttccaa
     3421 aaaagcccgc atatctttta tattggcaga ttcctcttcg gtgggtattt tatccttcag
     3481 atccccaggg atgtattcgg acagagattc ttttatctct ttaggtagcc atattatccg
     3541 ttcgtatccc ccgtcggctt gtaaaaactt aggtgaacgc atgtattcca ggctaagccc
     3601 agtgaatcct tccacctgtt ttccacctga acactgcccg gccatggccg aaaatggtat
     3661 tcccagtggt gtttctcccc ggaaatcccg gtccacaatg ccaataccat ccagttccgg
     3721 gatgtagaat gccactgctt cgaaacatcc acaggaagtg tgggggtgtc cgaatacact
     3781 gtgcagatac actgtgtcga aggtaccctg tgaacgttgg gatacaacct cgtttaccgt
     3841 ggaatattct ccttttactt catctaaaac ttcacccttt tcaatttcaa atataggccc
     3901 atctggatcc attttagccg cggcacggca gtcgaaccag ttgatggctc cacacagggc
     3961 ggttcgatcc ggggtgacca cacacacgtg ggtgggtgca aaggactggc acatgacaca
     4021 accatagaaa acatccacat cttcatctga gagttcacga gcccgggcat ccctttgttc
     4081 atactggagt cgggcttttt ccacgaatct ctggacttcg tcttcatcag tcatgatggt
     4141 gatggctatt gattcaataa tagggtattc ctctttaaat aagattagta atgctttagc
     4201 aagatcttcc agtttaaacc ctgcttcaag agcttcttta ctaaccctgc accagatctg
     4261 gtccctctgg ttgagatgca tgaacccttt tatgtagttg caaagttcgt gggttctgcg
     4321 ctcaattaca ccctccagtt ccttctccaa tgcttctccc tggatttcga tctttatccc
     4381 caggggatgg atgtcccctt cccccatatc ctgcagttcc ggacctatga cttccacctt
     4441 gccatcttct acagtttctt ccacttgaac cagttccgcg ccccatgact tgggaccggc
     4501 cagctccaca aacatgttgg ctgatcttat tctttcaccc tcatacatgg ggcttacatc
     4561 aacgggtata tcctcaaaca tccaatttcc tccgttcatc ctctttttat atgatatcta
     4621 attattgtcc tgccatcttg gccagatatt ttaaccattc ttcatctttc atattgggga
     4681 aagatgcatc agcattggaa tggaagaact tgcaaatagt caaggtctta agatgggggg
     4741 catagtgttt cagtgccgat aatccttgtg aagccaggta atatatgcat cccaaaaaga
     4801 tcaccagatc atgttgtcct tccccgttta ctcccaccca ttcagggtct ttaaggagat
     4861 tgactatttc aatggcacca taagcaactg aatcaaggtc cttttccctg aatgctttgt
     4921 aagcatctcc ggtggttacc actggaagat cccatttttc agctataccc accgccaggg
     4981 aaagtagtgg ttcctcctta accagaggtc ctaaaacgag taaaggcctt tttgactttc
     5041 gtatcattaa ttccgcagtt tcaggtgtaa ccagaagggc ttgttttggt ccagcaataa
     5101 ccgttggttg ccagggtatt actctatcgt tactcaattt aatcaccatc taatcccatg
     5161 agagatggtt cctgtggtat ttccctaggt tcccatccta cttcttccag taagtctttc
     5221 acttcttttt tatgagtgat tggtatgtct ttgtcgttac ggacaaagag gttaatatcc
     5281 gggggaagga caccgaagta acgcttgtat agatctatat aatgatttag tttgatttgc
     5341 ctgcccttag cagtgtcgtt gggccttatg cacagcttag ctatcatggg catagactcc
     5401 tctactgttt cagctgcgta gatcaaatgt tctggcgctg gttctccatc cattattttg
     5461 ccagttctca gatcttttat tttccagtta tccttcttat ctgctcggcc cagataaagc
     5521 cgacgatact ttgatccatg tggccctatc accactggga taccccagcg gttaactcca
     5581 gtagcgattg ccgctgcttt ctggctgtag gcaccccagg ccaccccaca tgcaccgacc
     5641 ctgttcagga tgtagtcggc tatttcctcg aagttgcctt ccagtggttt tttggcgaat
     5701 atgttggcta ttttaataca tgcacctgag atatgggcat ttgatacaca tgaccccata
     5761 tttaccaggc cacgagcatc gaagtctcca ctgtactgtt catagagtgt ttttccttcc
     5821 tcgtctttgt attcaccaat ggacatggct ccacaacccg tggcaaccac aatgtagtta
     5881 cgttccaaaa actccttggc catttcagct acttctttcc caccatctgg atagtttgaa
     5941 caccccacaa aggctattac accaggtata tcccctagaa ctaatggagc tcctactctt
     6001 cggatttcta catcttgtac tggccctctt ccagcacgta tactgaattt ttcatcttta
     6061 acgaagtgtt c
//
LOCUS       DAOQVW010000026         6069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_14377_length_6069_cov_3.952611, whole genome shotgun sequence.
ACCESSION   DAOQVW010000026 DAOQVW010000000
VERSION     DAOQVW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14377_length_6069_cov_3.952611"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(715..1494)
                     /locus_tag="PKK85_01060"
     CDS             complement(715..1494)
                     /locus_tag="PKK85_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNS24647.1"
                     /translation="MSTIRILSWNVNGIRAVHRKGFKKWVLNDKPDVLCLQETKATLK
                     QFPKDIRNMDDYHLYTSEAERKGYSGVATYTRLKPEKVGHGLGISKFDQEGRTLITDY
                     GEFVLFNIYFPNGKMSEERLRYKLDFYDSFLVYADQLKDEGRNIVVCGDVNTAHKEID
                     LARPKENSKVSGFLPVEREWMDRFLSHGYVDTFREFNKEPEQYTWWSYRTRARERNVG
                     WRLDYFFVNQEFMSQVKNAYIMSDVMGSDHCPVGLEIKIKE"
     gene            complement(1607..3046)
                     /locus_tag="PKK85_01065"
     CDS             complement(1607..3046)
                     /locus_tag="PKK85_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNS24648.1"
                     /translation="MISREKVLKKPEAILDEYPRKGGSAPTATHYCPGCGHGILHKLI
                     GEAIDSLGIQDRTVMTSPVGCAVFAYYYFDCGHVQVAHGRAPAVGTGLSRAEDDAIVI
                     LYQGDGDLASIGLNETIQAANRGEKMAVFFVNNTVYGMTGGQMAPTTLVGEKTITCPT
                     GRDPSYTGYPMHMCELLDNLQAPVFIERVSVSDPKHIRKARRAVKKALEIQRDGKGYA
                     FVEVLSPCPTNLRLDVKGTEDFINHEMAREFPLKNFRNRSHEVEPLCRAQSDFSLEAL
                     NHIFEVDQESTMEAIDDPEFQCKVVKMAGFGGQGVLSLGLTLAQAACKDRKHVSYYPS
                     YGPEQRGGTAHCTVIIDGQDIGSPYVHYNDVLVAFNRPALNKFSDRVKDGGYIIYDSE
                     VGEFDPPKGVKAIGVPAFKIAKDNGVKKAGNTVMLGAIMELGLLELPPDVFKSAIEHT
                     FHRKPQLIGVNLKILEAAAKWTHDNLVKE"
     gene            complement(3036..4106)
                     /locus_tag="PKK85_01070"
     CDS             complement(3036..4106)
                     /locus_tag="PKK85_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNS24649.1"
                     /translation="MATQLIKGNTAVVIGAMYAGCDCLFGYPITPATEILHEASLYFP
                     MVGRKVLQAESEEAAINMVYGAAAAGHRVLTASSGPGISLKQEGISFLAGAELPCVIV
                     DIMRAGPGLGNIGPEQSDYTQLVKGGGHGNYRNIVLAPNSVQEMCDMTIKAFHLAEKY
                     RNPAVVLADGVLGQMVEPLMFPEEALEPEIDTSWAVCGNRKTRKNLVTSIFLNFDQLE
                     SFNYRIQDKYQEIKENEVDYEEYYMDDAEVVVVAYGISSRIARSAVDWARENGLKAGL
                     FRPKTLFPFPEKELLNISKRKNCKFISVEMSNGQMMEDIILAIRCQRSVELVNRMGGN
                     LMDQKSIVDKIRELSGDDHNDQ"
     gene            complement(4106..4366)
                     /locus_tag="PKK85_01075"
     CDS             complement(4106..4366)
                     /locus_tag="PKK85_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNS24650.1"
                     /translation="MFKEKKDPYPVINNLECKGCERCIIACREGVLKMSDEVNQRGYH
                     YAVYSGQGCTGCGDCYYTCPEPLAVEVHIPRKERKAQLQEEE"
     gene            complement(4433..>6069)
                     /locus_tag="PKK85_01080"
     CDS             complement(4433..>6069)
                     /locus_tag="PKK85_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS24651.1"
                     /translation="FDSYHEYKENFHVEPPNNFNFAYDVVDEYACLYPEKKAMVWCND
                     DEDYTFTFKDLKEYSNRAANFFAKYGVKAGDRVMLTLKSRYDFWFCILGLHKLGAITI
                     PATHMLKTKDIVYRLQKAGIKMVVCIAEEGVPEYFDEACAQLGDYELIKSFVGDQDRD
                     GWLNFREEINNMSPEFQRPSREDQNSNEDISLIYFSSGTTGMPKMIMHDYTYPLAHII
                     TAKYWQNVLEDGLHYTVADTGWAKAMWGQIYGQWIAGSAIFVYDYDRFDAAQMLEKAS
                     THGVTTFCAPPTIYRFLIKEDLSQYDFSTLQYAVTAGEPLNPEVYNKFKKFTDLQLME
                     GFGQTECVICIANFPWMKPRPGSMGKPSPRYEISLMDADGNTCEVGEEGEIVIKTADG
                     KPPGLFSGYYRDKEKTERAWYDGYYHTGDTAWMDEDGYFWFVGRNDDMIKSSGYRIGP
                     FEVESAVISHPAVLECAITGVPHPIRGQVVKATVVLTKGYKPTDDLKKEIQNHVKQVT
                     APYKYPRVMEFVDELPKTISGKIRRIEIREKDQKDS"
BASE COUNT         1584 a         1483 c         1166 g         1836 t
ORIGIN      
        1 atacagtttt tatccattct aaaaaaaatg tattcacctt tctcattttt ttctaatttt
       61 acaaattaaa aaagttcata tttgaacttt gtacattact taacagttaa attgtttatt
      121 ttaggagtta attagctcat agtttttcat cttcttcact atcaccctaa tcataaaaat
      181 cattacatca ttttagttcc ataggatctt cattgttctc ctgtttctga gatacaaatg
      241 atgaatgatg aatcaattat tttatacctt caacctaaac tttaaattta tcttaaaaag
      301 ctcgataaat aaacctataa tcttgcagat cccaactgtt ttgtgcgaag atcagcagtg
      361 tactatttgt ttatatgctt agcttcaaac aaatagttgg catatagctt cttcaagcat
      421 catacaagta aaatggtagg gccgccaatg gaagaactct ccatcaattc tcattgactt
      481 gctgatgtat aggatgaatc taatcgtaag gtggaagaaa aggatcatga aaaacttcta
      541 ctgaatatcc ccaaaaaaat gaaaagtgta gaaatgggtg aaagtggcat ggatatgatt
      601 cgtgaaatgt tttgataagc tccaaaacgt agagatagaa agtttataca tcatttatgc
      661 aatatgaaac cttaattacc ttatgctgag gacattttcc ctttgaacat caaatcattc
      721 tttaattttg atttccaacc ccactggaca atgatcagaa cccatcacat cagacatgat
      781 gtaggcattc ttcacctgac tcataaactc ctgattcacg aagaagtaat ctagacgcca
      841 ccccacattc ctctcccggg cccgggtgcg gtaactccac caagtatatt gttccggttc
      901 tttattaaac tcacggaagg tgtccacgta accgtggctt aaaaacctgt ccatccattc
      961 acgctccacc ggtaagaatc ctgaaacctt cgagttctcc ttaggcctag ctaagtctat
     1021 ctctttatgg gcagtattca catccccgca taccacgatg ttacgccctt catccttcaa
     1081 ctggtcagcg taaactaaga aagagtcata aaagtctaac ttataccgta acctttcctc
     1141 agacatctta ccattgggaa aatagatatt aaaaagtaca aattccccgt agtcagtgat
     1201 gagggttcgt ccttcctggt caaatttgct gatgcccaat ccatgaccca ctttttccgg
     1261 ttttaaacgg gtgtaggtag ccaccccact gtaccccttt ctttctgctt ctgaggtata
     1321 aagatggtaa tcatccatat tacgtatatc cttagggaat tgtttcaatg ttgctttagt
     1381 ttcttgcaag cataaaacat cgggtttatc atttaatacc cattttttaa agcctttccg
     1441 gtggacagcc ctaataccgt ttacattcca tgataaaatc cttatggtgc tcataaaaaa
     1501 tctccatatt tctttgaatc tgcaggattg tttttcataa gtgatttaag atcccaataa
     1561 aacctatatc tcccatgttt tccttaaaaa aggaatgtaa ggagttttat tcctttacca
     1621 gattatcatg agtccatttg gctgctgctt ccagtatttt caaattgacc cctatcagct
     1681 gtggcttacg atgaaaggta tgttctatgg cacttttgaa tacgtcaggg ggaagttcta
     1741 ataggcccag ttccattata gctcccaaca ttacggtgtt ccccgccttc ttaactccat
     1801 tatccttggc aattttaaaa gctggcacac caatggcctt aacacctttg ggtggatcaa
     1861 attcacctac ttcggagtca taaataatgt aaccaccatc tttaacccga tccgaaaatt
     1921 tgttaagggc tggacggttg aaagccacca atacatcgtt atagtgaaca tagggcgagc
     1981 ctatgtcctg tccatctatg atcactgtgc aatgggccgt tccaccccgc tgctcagggc
     2041 cgtaggacgg atagtaggat acgtgctttc gatctttgca ggcagcttga gccagagtaa
     2101 gacccagact caacactccc tgtccaccaa aacctgccat tttaaccact ttacactgga
     2161 attctgggtc gtcaatggct tccatggtac tctcctgatc cacctcgaag atgtggttca
     2221 gtgcctccag gctgaaatca ctttgggccc ggcataatgg ttctacttca tgacttcggt
     2281 tccgaaagtt cttcaaagga aattctcggg ccatttcatg gtttatgaaa tcttctgtcc
     2341 ccttaacatc cagtcggagg ttggtgggac agggtgatag tacctccaca aaggcatatc
     2401 ctttaccatc cctctggatc tctagagctt tcttcacggc ccttcttgct ttacgtatgt
     2461 gtttgggatc cgagactgat actcgctcga taaacacagg agcttgcaag ttgtccagaa
     2521 gttcacacat gtgcatggga tacccagtat aactggggtc tcttccggtg gggcaggtga
     2581 ttgtcttttc accaaccaag gtggtgggtg ccatctgccc accagtcata ccataaactg
     2641 tgttgttaac gaagaacact gccatctttt ccccccggtt agcagcctgt atagtttcat
     2701 ttagacctat agacgccagg tctccatctc cctgatacaa aatcacaatg gcatcatctt
     2761 ctgcacggga caggcccgtt cccaccgcag gcgccctgcc atgagccact tgcacatggc
     2821 cacagtcgaa gtagtagtag gcgaaaacag cacagcccac cggactggtc attaccgtac
     2881 ggtcctggat ccccagtgaa tcgatagctt cacctatgag tttatggaga attccatggc
     2941 cacatcccgg gcagtagtgg gtggcggtag gggcgctgcc ccccttgcgg gggtactcat
     3001 ccaaaatggc ttccggtttt ttcaacacct tttccctact gatcattatg atcatctcca
     3061 ctgagctctc taatcttatc cacaatactc ttctggtcca tcaggttacc tcccattcga
     3121 ttaaccagtt ccactgagcg ttgacatcta atggccagga ttatgtcctc catcatctgg
     3181 ccgttactca tttccactga aataaactta caattctttc gtttagatat gtttagaagt
     3241 tctttttctg gaaaagggaa aagtgtcttg ggacggaaca atcccgcctt caaaccattt
     3301 tcacgggccc aatcaactgc ggatctagcg atgcggctgc taataccata ggcaaccacc
     3361 accacctccg catcatccat gtaatattcc tcataatcca cttcattttc ttttatttcc
     3421 tgatacttat cctgtatacg ataattaaat gattctaact ggtcaaaatt caggaatatg
     3481 ctagttacta gattttttct ggttttccgg ttaccacaaa ctgcccagga ggtatctatc
     3541 tctggttcaa gggcctcttc aggaaacatc aggggttcta ccatctggcc cagcacccca
     3601 tcagccagga caaccgccgg gtttctgtat ttttctgcca gatggaaagc tttgatggtc
     3661 atgtcacaca tttcctgcac tgaattaggt gccaagacaa tgttacgata gttcccatgc
     3721 ccacccccct taaccagttg ggtgtagtct gattgttccg ggccaatgtt acccagccca
     3781 ggaccagccc gcatgatatc cacaatgaca cagggtagct ctgctccagc tagaaatgat
     3841 attccttcct gtttaagact gatccccgga ccagatgatg cagttaaaac ccgatgtcca
     3901 gctgcagcag caccatagac catgtttatg gcagcttctt ctgattcagc ttgtaaaacc
     3961 ttccttccca ccattggaaa gtacaatgaa gcttcatgaa gtatttctgt ggctggagtg
     4021 atggggtaac caaataaaca atcacacccc gcgtacatgg cccctataac cactgctgtg
     4081 tttcctttga ttaactgggt tgccattact cttcctcctg taactgtgcc ttgcgttctt
     4141 ttctagggat gtgcacctcc actgctagtg gttcagggca agtatagtag cagtccccac
     4201 aacctgtgca tccctggcca ctgtacactg cgtagtggta tcccctctgg ttaacttcat
     4261 cactcatctt caaaacgcct tctctacagg caattataca tctttcacat cccttacatt
     4321 ctaaattatt aataacagga tagggatctt ttttttcttt gaacataata gatcttgcat
     4381 tcattaagta atttgaagta tttgaataat ctttgtatgg ccagattcct ggttatgaat
     4441 ctttctgatc cttttctctt atctcaatcc tgcgaatttt tccgctgatg gttttaggaa
     4501 gttcatccac aaattccatc actcgggggt atttataggg agcagtaacc tgtttaacat
     4561 ggttttgtat ctcttttttt agatcatcag taggtttata acctttagtt aaaaccactg
     4621 tggctttgac tacttgtccc cgaatcggat gaggaacccc agttattgcg cattccagaa
     4681 cagcaggatg ggatatgact gcgctttcca cctcaaatgg acctatgcgg tatccggaac
     4741 tctttatcat atcatcgttt cggcctacaa accagaaata gccgtcttca tccatccaag
     4801 ctgtgtctcc ggtatggtag tatccatcat accatgccct ttctgttttt tctttatcac
     4861 ggtagtatcc gctgaaaaga cccggtggtt tgccatcggc agtttttatc actatctccc
     4921 cctcttcacc cacttcacaa gtgtttccat cggcatccat caaggaaatt tcgtatctgg
     4981 gtgaaggttt ccccatggat cctggtctgg gtttcatcca gggaaaattg gcaatgcaaa
     5041 tcacacattc ggtttgccca aagccctcca ttagttgcaa gtccgtgaat tttttgaatt
     5101 tattgtaaac ttcaggattt aagggttccc cggcagtcac cgcgtattga agggtggaga
     5161 agtcatactg tgagagatct tccttgatga ggaaacggta gatggtgggt ggtgcacaga
     5221 aagtggtgac tccgtgggtc gaggccttct ccagcatctg cgctgcatcg aagcgatcgt
     5281 aatcgtatac gaagattgct gatccagcga tccactgccc atagatctga ccccacatgg
     5341 ccttggccca gccagtgtca gccactgtat agtgcaagcc gtcttctaaa acattttgcc
     5401 agtacttggc agttataata tgggccagtg gatacgtgta atcatgcata atcattttag
     5461 gcatgccagt agttcctgat gagaaatata ttagggagat gtcctcatta ctattctgat
     5521 cctcccttga aggacgttgg aattcggggg acatattatt gatttcttcg cggaagttaa
     5581 gccatccatc cctgtcctga tctccaacaa atgattttat gagttcataa tcccctaatt
     5641 gggcgcaggc ctcatcgaag tactccggca ccccctcttc cgcaatgcaa accaccatct
     5701 ttattccagc cttctgtagc cggtacacga tgtccttggt cttcaacata tgggtggcag
     5761 gaatggtgat ggcccccaac ttgtgaagcc ccaatatgca gaaccagaaa tcgtatctgc
     5821 ttttaagggt gagcataacc cgatctccag cttttacccc atatttcgca aagaaattag
     5881 cagcacggtt agagtactcc ttcagatcct taaaggtgaa ggtgtaatct tcatcgtcgt
     5941 tgcaccatac catggccttt ttctcagggt agaggcatgc atactcatcc accacatcgt
     6001 aggcaaagtt gaagttattt ggtggctcta cgtgaaaatt ctccttgtat tcatgataag
     6061 aatcgaact
//
LOCUS       DAOQVW010000027         6008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_14561_length_6008_cov_3.711406, whole genome shotgun sequence.
ACCESSION   DAOQVW010000027 DAOQVW010000000
VERSION     DAOQVW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14561_length_6008_cov_3.711406"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PKK85_01085"
     CDS             <1..1343
                     /locus_tag="PKK85_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HNS24652.1"
                     /translation="ASRSIKSSLQPIINLDLQMKWDDSLVPIINGRIITANGENIPIT
                     YRAALAMEPARKHPLSVRRIREQIQKTGGTIFNICKLEIDYPGGLFAPISTLNQLRRN
                     FLEEAEELLIKRHLPDGLEIGTIHHNLERVKRELTLDLQVPSPSTTPDLMVWISDPKS
                     LLKAVEEECKRAYLQAPMDLKSRKDGKKILKLMERAINMVDDSKTQLIWKWPLITPHS
                     LLKNLEPLLNELVELGLPGVMVDGLGAAEVVKNQERPPNLYGSTGLNIWNHLTMQELA
                     GAFNGLSISPELSRDDLMKLSRLKGLNADLEIMVQGNQEVLISQDTLGPADDGEKYDD
                     KVFWGIKDKKGHIFPVKWDHDHRTHVFNSVELCLIDHLPSLVNMGWGGLVIDARGKPV
                     AYTRDMIQLYQDALHLSLQNPPDLKGELRSLKKEVKMRSTGGITTGNFLRGVLN"
     gene            complement(1757..1906)
                     /locus_tag="PKK85_01090"
     CDS             complement(1757..1906)
                     /locus_tag="PKK85_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24653.1"
                     /translation="MYLLLHGVEGVYKGTCTVLPPGASQPPTPTQFLRILSGIILANT
                     LQVLY"
     gene            2218..4107
                     /locus_tag="PKK85_01095"
     CDS             2218..4107
                     /locus_tag="PKK85_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNS24654.1"
                     /translation="MELRNIKGVGERMAQRIEREFGSEDAFLDAALNCEVDRLARIEG
                     ISQRRAVEIINEVLGNPSQKFLKTERAIQLYEDIIKKILDHASTDYARNRVLLLSPTK
                     DPGTIQERMSRVMDAKRMVAELPQDEIQKLLIRVNPLEKPRPRVEPGHALLVESKKDY
                     ENLLERDLHRYISIITLEELESLHEYELVVYAYNEGFLEIDDAPNLAMVPLDAQDGEL
                     VPGKVLAYYQHNQETLENAAKLRDILGWSSILPDVMNTLCSIQSRRVDEASFNRTVDE
                     AKENADQKMKNAIKGVELSGQEVLDLLNSGMPGKIRDILDTVLDEARQEIYSKTGCSF
                     NPFLAKYPLEFDEEELARVREQEMGRQQLKAFEHQVMAAKELSQLHGKVEKEVREVLE
                     FDYQYTLGSFAYKYGLQEPVLADGFSFEDAINLDLALSRKVQRISYQLQYPDNVVLLT
                     GANSGGKTTLLETLAQISILTQMGLPVPVIMARVKLVDELYFFSKKRALDAGAFESFL
                     WNFVPIATQNTDKLVLLDELEAMTELEAAAKIISSFMGFIQDSGSYAVIVTHLAREIL
                     KHIQVRVDGIEASGLDDEYNLIVDRTPRMDYLARSTPELILRRVYEKSDGKVKDVYRK
                     MLELF"
     gene            complement(4157..4663)
                     /locus_tag="PKK85_01100"
     CDS             complement(4157..4663)
                     /locus_tag="PKK85_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNS24655.1"
                     /translation="MKATDFIGTVVIDKQAREVGKVADISVTIKKCLVDKVIVVTGSA
                     LKRNYFAVVSDEIAEIGDYMQLTLDEAGVEAKGKVDKIEQLPLEGDLLKTFLGKEVLS
                     EDALIIGKVEDMLIDPKGCLIHNVVIATGSTFRKKNLIVSDDDIKHIGDYVILEMKKE
                     KVEELLGD"
     gene            complement(4729..5313)
                     /locus_tag="PKK85_01105"
     CDS             complement(4729..5313)
                     /locus_tag="PKK85_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNS24656.1"
                     /translation="MRKILICLIFVVIFSLVQAPASVAWTSVTHDDFVEEVYYFLPAD
                     AQQKLNLELMKSASDDPDFKFFDYRFHHYPASCARADYWLDRGASAYEKGDYDQASYS
                     FGVASHYITDSFCAPHCVDGATGYHSLYEIQATTLNPHITIKSGNLDTLMADGHVKGQ
                     YSWNSWMTSRDSSYIQADLNRAASACYVAINNRI"
     gene            complement(5453..>6008)
                     /locus_tag="PKK85_01110"
     CDS             complement(5453..>6008)
                     /locus_tag="PKK85_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24657.1"
                     /translation="WPQYATFNRVRHIPVEMEILLLLVVFLELILADANSFYTRFNYY
                     DKFMHILVPAVLGLMGMMIIYTFYALGRLQASVGVMFAIIVIVVMAMGAALEMSEYFY
                     DQILYPSIGAWLPTGLTQGSHLAPPLDDTMQDLWADLFGAIIGASLGVWLIKRSEKEG
                     KEPTIVEELEAEIEMETASADEDT"
BASE COUNT         1750 a         1307 c         1370 g         1581 t
ORIGIN      
        1 gggcttccag atccataaaa agctcgctac aacccatcat taatctggat ctgcaaatga
       61 aatgggatga tagccttgta cccattataa atggacgcat aatcactgct aatggtgaaa
      121 acattcccat aacatatagg gctgctttgg ccatggagcc ggccagaaaa catcccctga
      181 gtgtgaggcg gatccgtgaa caaatccaaa aaactggagg caccatcttc aacatctgca
      241 agttggaaat tgattaccct ggtggcttgt tcgccccaat atccactttg aatcaattaa
      301 gaaggaattt tctagaagaa gctgaggaat tactgataaa gcggcacctt cccgatggct
      361 tggagatagg taccatccat cataatctgg agagggtaaa aagggagttg accttggatt
      421 tacaagtacc aagcccatcc acaaccccgg atctaatggt ctggatcagt gatccaaaat
      481 cactcttaaa agcagtggag gaggaatgta aacgggctta cctccaagcc cccatggatc
      541 ttaagtccag aaaggatggc aagaaaattc taaaattaat ggaacgggcc attaatatgg
      601 tggatgattc aaaaacacaa ttaatctgga agtggccttt aatcactcct cacagtcttc
      661 tgaaaaattt ggagccactc ctaaatgaat tggttgaact gggcctgcct ggtgttatgg
      721 tggatggatt aggtgctgct gaagttgtta agaatcagga aaggcccccg aatttatacg
      781 gttccacagg gcttaatatc tggaatcatc tgaccatgca ggaactggca ggagctttca
      841 atggccttag catttcacct gaactctccc gggatgacct gatgaagttg agccgtttaa
      901 agggtttaaa cgccgacttg gagatcatgg tccagggaaa ccaggaggtg ctaatcagcc
      961 aggacactct aggcccggct gatgacgggg aaaagtatga tgataaggtt ttctggggaa
     1021 tcaaagataa aaaaggacat atattcccgg tgaaatggga tcatgaccat cggacccatg
     1081 tatttaactc ggtggaactc tgtttgattg atcatctgcc cagcttagtg aatatgggtt
     1141 ggggagggct ggttattgat gcccggggaa aaccagttgc ctacaccagg gatatgatac
     1201 aactatacca ggatgccctg cacctatccc tgcagaatcc ccctgatttg aagggtgaac
     1261 ttcgaagcct taaaaaggag gttaaaatga gatcaaccgg tggaataacc accggcaact
     1321 ttttaagggg cgtgttgaat taaaaggaca gtttaagcca tccaaagatt tccttcattt
     1381 tctgggaaat ttaaatcaaa aaccaggata atacttagct taattgtttt tcgggtttgt
     1441 cagggggtta taacactgaa tgtaccaagg ctggtcccgg ctaagggccg ctagttgcac
     1501 cagattcata tagggggagg tattcccggg aatgataatc caagtagggg gtagattatg
     1561 attccctcca gggatgccat ggtaatattg ccgtgatagt aagctatggc tggtgaatcc
     1621 caatttttta caaaatggtg agtattagaa ccatgactat tccccaccag aactttaatc
     1681 acccaggaat cccctgccaa gtatgatgga gccttgggag attcttgatt cccctgattc
     1741 tgtttctcag tccatgttaa tacagcacct gcagggtgtt ggccaggatg attccagata
     1801 agatccttaa aaattgtgta ggagttggtg gctgcgaagc cccaggagga agaactgtac
     1861 aggtcccttt ataaacccct tccactccat gtaacaagag gtacatcccc ccggtatcag
     1921 gtcaatgcca agacaagtag acaaacaatt agatccattt ttttatattc ccccgaaatc
     1981 actatggatt tagtgtatta catgactttt attattttta acttctttaa cttcctctga
     2041 ctcaggctgg attccattaa acagcgaaat agtctggttc tatccggtaa agtccatatt
     2101 aattttttta ttactcgggt gaggtgttca taaaatgatt ccccacacat tcccgcccca
     2161 acccactcat aaaatgatta ataggtgtga agaacaagga tatttaaggt gtgaaatttg
     2221 gaactgcgta atataaaggg tgttggagaa cggatggccc agcgtataga gagggaattt
     2281 gggagtgaag atgcattcct agatgccgcc cttaactgcg aggtggatcg gctggctcgc
     2341 atcgagggga taagtcaacg tagggctgtt gaaattatca acgaagttct tggtaaccca
     2401 agccagaaat ttctaaaaac agaacgggcc attcaactct acgaagatat cattaaaaag
     2461 attctagacc atgccagtac tgattatgcc cgtaatagag ttctacttct aagccccacc
     2521 aaggatcctg gaacaatcca ggaaagaatg agtagggtga tggatgccaa aaggatggtg
     2581 gctgaactac cgcaagatga gatccaaaag ctcctaataa gggttaatcc cctggaaaaa
     2641 ccacgtcccc gggttgaacc aggacacgcc ctcctggttg agagtaaaaa ggattatgaa
     2701 aatcttttag aacgtgactt gcaccgttac atttccataa taaccctgga ggaattggaa
     2761 agccttcatg agtatgaact ggtagtttac gcctataatg aaggtttttt agaaattgat
     2821 gatgctccta acctggcaat ggtccccctg gatgcccagg atggagagct ggtaccagga
     2881 aaggtgctcg cctactacca gcacaaccag gagaccctgg aaaatgcagc taaactaagg
     2941 gatatcctag gctggagttc tatcctgccg gatgtgatga acactctctg ctcgatacaa
     3001 tctcgccggg tggatgaagc cagcttcaac cgaaccgtgg atgaggctaa ggaaaatgcg
     3061 gatcagaaga tgaaaaacgc cattaagggg gtggaattat ctggccagga ggttttagat
     3121 cttctgaaca gtgggatgcc cggtaaaatc agggacatcc tggatactgt tctggatgag
     3181 gcccggcagg aaatttactc caaaaccggc tgtagtttta atccattcct ggccaagtac
     3241 cccctggaat ttgatgagga agagttggcc cgggtccggg agcaggaaat gggacggcaa
     3301 caattgaaag cctttgaaca ccaggtgatg gcagccaagg aactttcaca gttacatggg
     3361 aaagttgaaa aggaggtgag ggaagtgctg gagtttgact accagtatac cctgggaagc
     3421 tttgcatata aatatggctt gcaggaacct gtcctcgccg atggtttcag ctttgaagat
     3481 gctataaatc tagatctggc cctttccagg aaagttcagc ggataagtta ccagctccag
     3541 tatcctgata acgtggtgtt attaaccggg gccaacagtg gaggaaaaac taccctgctg
     3601 gagacactgg cccagatatc catactgacc cagatggggt tgccggttcc agtgataatg
     3661 gctcgggtga agctggttga tgaactatac ttcttttcca agaagcgtgc cctggatgcc
     3721 ggtgccttcg aatccttcct ctggaacttt gtaccaatag ccacccagaa cactgataaa
     3781 ctagtcctcc tggatgaatt agaagctatg actgagttgg aggctgcagc aaagatcata
     3841 tccagtttta tgggatttat ccaggattca gggagttatg ctgttattgt aacccatctg
     3901 gcacgggaaa tacttaaaca tatccaggtg cgtgtggatg gaattgaagc cagtggactg
     3961 gacgatgaat acaatttaat cgtggaccga actcctagga tggattacct ggcccgcagt
     4021 acgccggaac tcattctccg gagggtttat gaaaagtcag acggaaaagt gaaggatgtc
     4081 tacaggaaaa tgctggagct cttttaaatt ccaggaaaaa agtattcaaa aaaaataaag
     4141 ggaaacaaca attcaattaa tctcctaaaa gttcttcaac cttttctttt ttcatttcca
     4201 gtataacata atcaccaata tgcttaatat catcatcaga gactattaaa ttctttttac
     4261 ggaatgtgga tccagtggcg attactacat tatggatcaa gcaacccttg ggatcgatga
     4321 gcatatcctc caccttacca atgatgaggg catcttcgga cagtacctcc tttcctagga
     4381 atgtctttaa cagatcaccc tccaggggga gttgttctat tttatcaact ttgcccttgg
     4441 cttcaacacc cgcctcatcc agggttagtt gcatgtaatc cccaatttca gctatttcat
     4501 cgctcaccac tgcaaagtag ttccttttaa gtgcagatcc ggttaccaca atgaccttat
     4561 ccaccaggca cttcttgatg gtcaccgaga tatccgccac ctttccaact tcccgggctt
     4621 gtttatcaat caccacagtt cctataaaat ccgttgcttt catcaaattc tacctcccta
     4681 aaggcttttt taaacaattg tccatgagct tggcatacaa cagtactact aaattcgatt
     4741 atttattgcc acgtaacagg cactggccgc ccggttaaga tcagcctgaa tataggaaga
     4801 atctctgcta gtcatccaac tattccaact atattgtcct ttaacatgac catcagccat
     4861 aagagtatcc aaattacctg atttaattgt tatgtgcgga tttagtgttg tagcctgtat
     4921 ttcatagagg gaatggtatc cggtggcacc atctacacag tgaggggcgc agaagctgtc
     4981 agttatgtaa tgggaagcca ctccgaatga gtagctggcc tggtcataat ctcctttctc
     5041 ataggctgat gctcccctgt ccagccagta atcagcccgg gcacagctag ctgggtagtg
     5101 atgaaaacgg taatcaaaga atttaaagtc aggatcgtca gaggcacttt tcatcaactc
     5161 caaattcaat ttttgctgtg catctgctgg taaaaaataa tagacttctt ccacaaagtc
     5221 atcatgggta acactggtcc aggctaccga ggcgggcgct tgtaccaggg aaaagatcac
     5281 cacaaaaatc agacagatta atatttttct catgttaata ccggtaatgc ctatttagta
     5341 gctttataaa aaaatgtcct ttatatttat tacattaatt caattaagga tgtgttgaat
     5401 tcgattagga tataaagtta tcgtttccca aaatttacag attttttttg tttcaagtgt
     5461 cttcatctgc actggcagtt tccatttcta tttccgcttc cagctcttca actatggttg
     5521 gctcctttcc ctccttttcc gatcttttta ttagccaaac cccaagtgaa gcccctatga
     5581 tggctccgaa taggtcagcc cacagatcct gcatggtatc atcaagtggg ggtgccagat
     5641 gagaaccctg ggtaaggcca gtgggaagcc aggctccgat ggaagggtac aggatctggt
     5701 cgtagaagta ttcagacatt tctaaagcag cgcccatagc catgaccact ataacgatga
     5761 tggcaaacat gacccctaca ctggcttgca gacggcccag tgcataaaag gtgtagatga
     5821 tcatcatccc cattagaccc aaaactgccg ggaccaggat gtgcatgaac ttatcatagt
     5881 aattgaatcg ggtgtaaaaa ctgttggcat cagccaagat cagctctaaa aacaccacca
     5941 gcaataataa aatctccatc tccactggaa tatgacgtac ccggttaaaa gtggcatatt
     6001 gtggccag
//
LOCUS       DAOQVW010000028         5957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_14718_length_5957_cov_3.752457, whole genome shotgun sequence.
ACCESSION   DAOQVW010000028 DAOQVW010000000
VERSION     DAOQVW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14718_length_5957_cov_3.752457"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKK85_01115"
     CDS             complement(<1..444)
                     /locus_tag="PKK85_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNS24658.1"
                     /translation="MKKEAMLYEKVGPMLNCHVCNRRCLISPGKRGFCNTRENVEDTL
                     YSLNYAAASSMAIDPIEKKPLFHFYPGTTSFSLGTIGCNFRCLYCQNWNISQAELEDI
                     PTRELPPEEAIQLSKENNCLSISWTYNEPTMWFEYTYDSAILAQKA"
     gene            complement(455..1504)
                     /gene="thiL"
                     /locus_tag="PKK85_01120"
     CDS             complement(455..1504)
                     /gene="thiL"
                     /locus_tag="PKK85_01120"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNS24659.1"
                     /translation="MPSKKLEISDVGEKRLIKRLLSRSRRSIPHSLFFKNFFDGNSLK
                     SLDDDAALLSLGDQYLVVTSDLLFEPSHFPMKMSPLEKGMKSVVVNLSDLAAMGARPL
                     GIFMSMGLPPNLLLEDFDLLVEGILKTCEDYDIALLGGDTNQTNEITLCGTALGMVDQ
                     DKVLMKYGAQKGDLVAVTGNLGSAAAGFEVLSLDDSVMEVFNPGEKDEILKTALQPEA
                     RVKEGRILAESNATSCTDISDGLVSELGEIIAANEEKIGIRFYEDKIPIRPILDVVAR
                     ISGKNIREMVMYYGEDFELLFTINSKDFGYLKEKMEVHVIGEVTSSGRIEMIDKGGET
                     NILVPRGYEHLNKQK"
     gene            complement(1494..1949)
                     /gene="cfbA"
                     /locus_tag="PKK85_01125"
     CDS             complement(1494..1949)
                     /gene="cfbA"
                     /locus_tag="PKK85_01125"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HNS24660.1"
                     /translation="MDTNSNLKSDVGVVLVGHGSRLPYGKDVLIQLAQIYNENTNHHV
                     EIGFMNMDKPSIPTSINKLADMGVKKIVVTPVFLADGVHTTQDIPRILGLDNGNESQE
                     HNHHHHHHHHHEEEEEEHIHFHGEIIYTKPLGADRRIADIIKDRVDNAL"
     gene            complement(1921..2319)
                     /locus_tag="PKK85_01130"
     CDS             complement(1921..2319)
                     /locus_tag="PKK85_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNS24661.1"
                     /translation="MKDIVRGWRHIPQRYNLIGSKCNHCGAVYFPVRVICPECRRKGK
                     LEEIKLSGKGKIHTYSVIHTPTGDFKLISPYVVAIIELEEGTKITSQIVDSDGDNIKI
                     GDEVELVFRKIREEGEDGVISYGYKFKLKK"
     gene            2613..3590
                     /locus_tag="PKK85_01135"
     CDS             2613..3590
                     /locus_tag="PKK85_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24662.1"
                     /translation="MDNDGYVMTGTSFLLILPVLILFIVLVELNYEDTESEVQIMESE
                     KILQVFQDLEFNIPRLTRQVLKEEAYFVISSKKPFIDVRKITRDNLQFKVDQITQNYD
                     KCGLQVNCTVLSIKESDDPFEVELTSLVVVQKDNVSHQEILQQKISIIDPYDPLPDPL
                     PFVKCNEYGGASINGSRIYYAHSLVLYLEQRGEKNGMVYENASSPLFINQCPYDPFVC
                     HGDPVTMETLKNCLDNGFYHESNDGACYLCRLEGKNTCPHQGLEVFILPPPTNNQSDT
                     APCSVDHVLFGEGGEIYPGVSIQYHQEENLIFQLFLDDSHRKKYGLPLI"
     gene            3648..4613
                     /locus_tag="PKK85_01140"
     CDS             3648..4613
                     /locus_tag="PKK85_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24663.1"
                     /translation="MDLLLAMVVFTVLLGVSANAMDMVSSKMDEASYIHSLERIVTEN
                     INILLNTPGSPENWEEGHLPMSMVKPGLAQIDPATGTVNQTLSMKKILKLAENYNELM
                     DGKVLPFGLKSTMIVSPRDSSLNTITISDQEHGETASDILIINRIVMCDYMMGNKLTS
                     IISHPASDASATELTQPICPHCNLTLNFQHPWQEPGYNKPGWKCKPFTITQNDIKTTD
                     IYLMTDPPQILDKSACWILDSTQNLTDLRFMFTTSPQHLNDKLSQLLGGKESATIWLH
                     VFSSGNSDEAFDVYIGRFSKNTPVENIKTQYLTPQPCNFVFKVCI"
     gene            complement(4675..5271)
                     /locus_tag="PKK85_01145"
     CDS             complement(4675..5271)
                     /locus_tag="PKK85_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS24664.1"
                     /translation="MLEYKGLYFNTHPQVYEPAEDSFLLAEHLELNRGDEVLEIGTGT
                     GLITVCAARKVAWVVATDVNPYAIKCASKNLITNRTFNVELREGNLFEPVMGEKFDLI
                     LFNTPYLPTHEDEELDEDINTAWNGGSDGRKIIDNFLDALCDHIREGGRVQLVQSSLS
                     GVEKTIERLEEMGFQAKVTARKKCFFEEIVVITGKLSS"
     gene            complement(5300..5617)
                     /locus_tag="PKK85_01150"
     CDS             complement(5300..5617)
                     /locus_tag="PKK85_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS24665.1"
                     /translation="MEKNPYKIFQKECPDLAEIFQGLVDVQRSRKGLDPKTKQLINIA
                     IQTAHSNAQGVKLHAQMAQKIGANREEVKGAVVMNLHLSGLSTVLNCLPAALDGYDRV
                     STD"
     gene            complement(5667..>5957)
                     /locus_tag="PKK85_01155"
     CDS             complement(5667..>5957)
                     /locus_tag="PKK85_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HNS24666.1"
                     /translation="RPRVSSAVVKLKPKKDVYLDEFFFKITRALFQHKRKKAKKALLD
                     SYHEWAPDKKVAREMLSRIDPKLMEKRPFQMHPENIIKISMELKILMAKNKL"
BASE COUNT         1669 a         1229 c         1143 g         1916 t
ORIGIN      
        1 tgccttttgt gctaatattg cggagtcata ggtatattca aaccacatgg tgggttcgtt
       61 gtatgtccag gagatagaaa gacagttatt ttctttgcta agttggatgg cctcttcggg
      121 tgggagttct ctggttggaa tatcttctaa ttctgcctgt gatatgttcc agttctgaca
      181 gtacaggcaa cggaagttgc accctatagt tcccagagaa aaggaagtgg tacccggata
      241 aaagtgaaat agtggctttt tttctatggg gtctatggcc atggaagatg ctgcagcata
      301 gtttaagctg taaagagtgt cttccacgtt ctcacgcgtg ttacagaagc ctcttttccc
      361 tggggaaatg agacatcgcc ggttacacac gtgacagttc aacataggcc ctactttttc
      421 gtagagcatg gcttcctttt tcatggctac ccacttactt ttgtttattc aggtgttcat
      481 aaccccgtgg taccagtata tttgtctctc cccctttatc tatcatctcg atccgtccgg
      541 aactggtaac ctcaccaatg acatgtacct ccattttttc ctttaaataa ccaaaatctt
      601 tggaattgat ggtgaaaagt aattcaaagt cttctccata gtacataacc atttccctaa
      661 tattttttcc acttattcta gcaacaacat ctaaaatggg tcttattgga attttatcct
      721 catagaaacg tattcctatt ttttcttcgt ttgctgcaat aatttctcca agttcactta
      781 ccaggccatc gcttatatcg gtgcaggatg ttgcattact ttcagctaaa atccgtccct
      841 ctttcactcg tgcctccggt tgtaaggcag tttttaatat ttcatccttt tcccctggat
      901 taaagacttc cataacagaa tcatcgaggg atagaacttc aaaaccagca gcagcagaac
      961 ctaaattccc agtcactgcc accagatccc ctttttgagc tccatacttc attaaaactt
     1021 tatcttgatc taccatgccc agtgcagttc cacacagagt tatttcgttt gtttggttag
     1081 tatctccgcc caaaagagct atatcatagt cttcacacgt ttttaatatt ccttctacca
     1141 gtaaatcgaa atcttcaagt agaagattag gagggagccc catggacatg aaaattccca
     1201 aaggacgtgc acccatagca gctaaatcac taagattaac taccacggat ttcattcctt
     1261 tttctagtgg agacatcttc atagggaagt gtgatggctc gaagagcaag tctgaggtga
     1321 ctaccaagta ctggtctccc agtgatagga gggcggcatc atcatccaga cttttaaggg
     1381 aattaccatc gaaaaaattt ttaaaaaaaa gggaatgtgg aatggacctt cgactccgtg
     1441 ataagagtcg tttaatcagt cttttttcac caacatcaga aatttcaagc ttcttagagg
     1501 gcattatcta cccgatcttt aattatatct gctatacggc ggtctgcacc aagtggttta
     1561 gtgtagatga tctcgccgtg gaaatggata tgttcctctt cttcttcttc gtggtggtga
     1621 tggtggtggt gatggtggtt gtgctcctgg gattcgtttc cattgtccag gcccagaatt
     1681 cggggaatgt cttgggtggt gtgaacaccg tcagccagaa agacgggggt aaccactatc
     1741 tttttcactc ccatatccgc cagtttattt atggatgtgg gtatggatgg cttgtccata
     1801 ttcataaatc ctatttctac gtggtggttg gtattctcat tgtatatctg tgctaattgg
     1861 attaaaacat ccttaccata gggcaaccgg cttccatgac caactaatac tactcccacg
     1921 tcacttttta agtttgaatt tgtatccata agatatcact ccatcttcgc cttcttctct
     1981 gatttttcta aatacaagtt ctacttcgtc accaattttt atgttgtctc catcggaatc
     2041 cacaatttga ctggttattt tagttccttc ctctaactct ataatggcca ctacataggg
     2101 tgaaattagt ttgaaatcac ctgtgggggt gtgtataacg gagtatgtgt gaatttttcc
     2161 ttttccactt agttttattt cctctaattt gccttttctt ctacactctg gacatattac
     2221 ccttacaggg aaataaaccg ccccacaatg gttacatttt gaccctatga gattgtaacg
     2281 ttggggaatg tgacgccagc cccgtacaat atctttcatt ctgaacctcc atagtaaaaa
     2341 tttgtgtaac tactattttt tattaactag taaatagttc tttgaatggt tttaatatct
     2401 caaaagaggt taatacttaa tctgaaagtg ttaccttacc actcatatcc tactttttca
     2461 cctattaatc tatttatgaa ttatatttaa aaaattatta ttaagggttt tttctctgta
     2521 tttttgtgtg gactaaactt aaatagtatt actttaataa taattagtag tattattgtg
     2581 ggggggtaaa aataaaatta ggagatgccg agatggacaa tgatggctat gtaatgactg
     2641 gaacgtcttt tttactgata ttacccgttt taatattgtt tatagtgctt gttgagttga
     2701 attacgagga cactgaatcg gaagttcaaa ttatggagtc tgaaaaaatc ctgcaggtct
     2761 tccaggattt ggaattcaat attccacgcc tgacccgtca ggtattaaaa gaagaagctt
     2821 attttgttat tagctcaaaa aaacctttta ttgatgttag aaaaataacc agggataatt
     2881 tgcaatttaa agttgatcaa attacacaaa attatgataa atgtggatta caagtgaact
     2941 gcacagtact atcaatcaaa gaatctgatg acccctttga agtggagtta acatccctag
     3001 tggtagttca gaaggataat gtttcacatc aagagatact tcaacagaaa atttcaataa
     3061 tcgatcctta tgatcccctt cccgatcctc tgccctttgt taaatgtaat gaatatggcg
     3121 gagcctccat aaacggtagt agaatttact atgcccatag tttggtgttg tatttagaac
     3181 aaagggggga aaagaatggg atggtttatg aaaatgccag ttcaccttta ttcattaacc
     3241 aatgtcccta cgatccattc gtctgccatg gagatccggt taccatggag accctgaaaa
     3301 actgccttga taatggattc tatcatgaaa gtaatgatgg ggcttgttac ttgtgtagat
     3361 tggaagggaa gaacacttgt cctcatcagg gacttgaagt tttcatttta ccacctccaa
     3421 ccaacaatca atctgatact gccccctgtt ctgtggacca cgttttattt ggagagggtg
     3481 gtgaaatcta tccgggggta agtattcaat atcatcaaga ggaaaatctc atatttcaac
     3541 tatttttaga tgactcccac cggaaaaagt atggattgcc cctgatttag tgtgattaca
     3601 tgtctgggaa cgatggacca gataaagagg ggggtcaagt attatccatg gatttattac
     3661 tggccatggt ggttttcact gtacttttag gagtttctgc aaatgcaatg gacatggtta
     3721 gttccaaaat ggatgaagct tcttatatac attcattgga gagaatagta acggaaaata
     3781 taaatatatt attgaataca cccggatctc cggaaaactg ggaagaaggt catcttccaa
     3841 tgtccatggt caaacccgga ttggcacaaa ttgatcctgc cactggaaca gtaaaccaaa
     3901 ctttgagcat gaagaagatt ctaaagctgg cagaaaacta taatgagctt atggatggta
     3961 aagtactccc atttggctta aaatcgacca tgatagtatc tccccgggac agttccctga
     4021 ataccatcac aatcagtgac caagaacacg gggaaacagc aagtgatatt ctcatcataa
     4081 accgcatcgt aatgtgtgat tatatgatgg gcaataaatt aacatccatc atatctcacc
     4141 ctgcatcgga tgcatcagcc acagaattaa cacaaccaat atgtccccat tgtaatctca
     4201 cactcaattt tcaacatccc tggcaggaac caggttataa taagcctgga tggaaatgta
     4261 aaccttttac tataactcaa aatgatatca aaactacaga tatctatctt atgacagatc
     4321 ctccccaaat attggataag tcagcctgtt ggatactgga ttccacacaa aacctcacag
     4381 accttcgatt tatgtttacc acatctcccc agcacttgaa tgacaaactt tcacaactcc
     4441 tgggtggaaa agaaagtgca accatatggc tccatgtttt ttcttctgga aattctgatg
     4501 aagccttcga tgtttatatt ggcagatttt ccaaaaacac accagttgaa aatatcaaaa
     4561 cccagtattt aactcctcaa ccttgtaatt tcgttttcaa ggtatgtatt tagccttaat
     4621 ggcgttgctc ttgtattgag ggttgtataa acttgggtga ataaaacatt caagtcagga
     4681 gcttaatttc ccggtgataa ccactatttc ttcaaaaaaa cattttttcc gagcagttac
     4741 ctttgcttgg aatcccatct cctctaatct ttcaatggtc ttttccacgc cagagaggga
     4801 agactgcacc aactgaaccc gacccccctc acggatatga tcgcataagg catctaaaaa
     4861 attatctata atttttctcc cgtcgctacc tccattccat gccgtgttta tatcctcatc
     4921 caattcttca tcttcgtggg tgggtaagta gggggtgttg aagaggatga ggtcaaattt
     4981 ttctcccatg actggttcga aaagatttcc ttcacgtaat tccacgttga aggttcggtt
     5041 ggtaatcaga ttcttggagg cacatttgat tgcataggga ttgacatcgg tggccaccac
     5101 ccatgccacc ttcctggcgg cacaaacagt gattaatccg gtgcccgttc ctatttcaag
     5161 aacttcatcg ccacgattta attcaaggtg ttcagccagc aaaaagctgt cttcagcagg
     5221 ttcatagact tgggggtggg tgttgaaata caatccctta tattctaaca taattctcac
     5281 caggaatctt tagtttattc taatcagttg acacccggtc atagccgtca agagctgccg
     5341 gaagacagtt aagaactgtg gagagtcctg aaagatgtaa attcataacc acggcccctt
     5401 taacttcttc ccgattggct ccgatttttt gggccatttg ggcgtgtaac ttcacacctt
     5461 gggcattgga gtgggcggtc tgaatggcaa tgtttattaa ttgcttggtc ttaggatcca
     5521 accccttcct ggaacgttga acatctacta atccctggaa aatttctgct aaatctggac
     5581 attctttctg aaagatttta taaggatttt tttccattga taatctctcc ctaaattctt
     5641 attgggttat ccagttgatt caataattat aatttgttct tagccattag tattttcagt
     5701 tccatggaga tctttataat gttttcgggg tgcatctgaa atggccgttt ttccattaat
     5761 ttggggtcta ttctggataa catttctcta gcaacttttt tatccggggc ccattcatga
     5821 tatgaatcaa gcagtgcttt cttggccttc ttacgtttgt gctgaaaaag tgcccgggta
     5881 attttgaaga aaaattcatc caagtagaca tcttttttgg gcttcaactt caccacagct
     5941 gaagatactc gtggccg
//
LOCUS       DAOQVW010000029         5927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_14810_length_5927_cov_3.341110, whole genome shotgun sequence.
ACCESSION   DAOQVW010000029 DAOQVW010000000
VERSION     DAOQVW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14810_length_5927_cov_3.341110"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1015
                     /locus_tag="PKK85_01160"
     CDS             302..1015
                     /locus_tag="PKK85_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS24667.1"
                     /translation="MSLALRWYPPAWVQIKTREKTIYLDPSYLKKYFKDHPTAREYSN
                     WPDEVDGLPDDLEKADIILVTHHHKDHVKKVTVDRISGPDTLIMAPGRVEKALGNRVR
                     VVAPGDELELDEVKIKVVPAYNTPEGSSTRKFHKLGEGVGYLLEIEGHVIYHAGDTDL
                     IPEMDGLGPVDVAFLPIGGTYTMNVEEAAQAALRIQPSLVVPIHHLQADPQDLQVELR
                     GGGMKVRVLRTGELLMIGE"
     gene            complement(1101..1793)
                     /locus_tag="PKK85_01165"
     CDS             complement(1101..1793)
                     /locus_tag="PKK85_01165"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNS24668.1"
                     /translation="MLPELLNQEEIEERSAQLENWKIIENHHLVGSYQFVNFASALEF
                     TRRVGEVAEDLQHHPEIDLEWGQVEITIFTHDRDGLTDLDFQFAIRVEEAFNPREGDE
                     VAKFMSLLEGGDLLEKREAARKLGALKDARALPLLIRALSSDDAALRRRAARSLGRLN
                     DKRAVDPLIYLLENGDDLLRQYAQDSLVELDRLAVPGLLKALESQDRRKRKLVVEALL
                     EISDEENPQLNR"
     gene            complement(1841..2323)
                     /locus_tag="PKK85_01170"
     CDS             complement(1841..2323)
                     /locus_tag="PKK85_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNS24669.1"
                     /translation="MENNRFKGRGKSLLVLYSYHHHNTEKIAKVMAEVLNAEIKMPDQ
                     INPDELQEYDLVGFGSGIYSAKHDESLLELADVIPEVNNKNAFIFSTAGITGKSKSAK
                     DHSKLREKLVSKGYTVIDEFQCKGFNTNSFLRLFGGMNKGRPNTEDLKNAEEFAKSLI
                     "
     gene            complement(2450..3193)
                     /locus_tag="PKK85_01175"
     CDS             complement(2450..3193)
                     /locus_tag="PKK85_01175"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNS24670.1"
                     /translation="MDPYRKTGVTVGVLFISATVASILGSVFIGSILEAPNYLTGISA
                     HGSGLIVGVLFFLIAAISAVATSFMLFPILRKYIESLALGYVVLRTFENVLYVLSAMS
                     LLVMLTVSQKYAAGAVDASFYPALGTLLLALQSWAILLGCLILFGLGSMALNYVLYQS
                     KLVPRWLSAWGLIGAALAVLYGLLGIFGVGIGFDFGNDILFMLASPYALLGVPIHVQE
                     MVFAVWLIVKGFNPSVIQLSLEKHNAKRS"
     gene            complement(3253..4143)
                     /locus_tag="PKK85_01180"
     CDS             complement(3253..4143)
                     /locus_tag="PKK85_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24671.1"
                     /translation="MMNIKEIKGIKLAPFTRMTASIYAVLGFVAAVVMLIGLTIVQVA
                     GVIPELGHFNLVTGVGIPLIVLLPIGAFFSTIVMSFFSVMLYNVLVPRLGGVKLELVG
                     NEVENIPVVPFSLILSAIGAVWAFILGLVLAAVISPLFSLIGAMGTMPEAASITANLT
                     ASGVAIPTGPGIGVAEAIVSLVLIIGLPIMAFVFGFIWNALFALFYNYLVSRVAKIQL
                     EFGQITGSLHELKHIPVLPTALSVALVFTLLGLISGILSKNYGEFISNFVVYFIETAL
                     IALLYNYLAPRIGSIKLNLE"
     gene            complement(4781..5074)
                     /locus_tag="PKK85_01185"
     CDS             complement(4781..5074)
                     /locus_tag="PKK85_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNS24672.1"
                     /translation="MNKVFLWWLIAGSKGGENRARIILELNNRPYNANKLAQKLSLDY
                     KTIRHHIDVLKENNLVKSTGETYGALYFLSDEMESVYDTFLEIWEEFKENEDK"
     gene            complement(5455..>5927)
                     /locus_tag="PKK85_01190"
     CDS             complement(5455..>5927)
                     /locus_tag="PKK85_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNS24673.1"
                     /translation="NYKTTAVEDEKLEKILEAARIAPSAANRQEWKFIVVKDQETRDK
                     LVEAANGQKFVGQAPATIVGCSTESERLMPCGQLAYTVDLSIAVSFMILEATELGLGT
                     CWLGAYDEEAVKKVLDIPDEIRVPAMFTLGYADENPDPRPRKELNEILCFEKFK"
BASE COUNT         1701 a         1330 c         1157 g         1739 t
ORIGIN      
        1 gatgagctga tgataccctt ccaccccctc cagggcttcc tcaaattcag gctggattat
       61 gatctctgat aactccgcct ggttccggcc ctgatgtggt gcttcacccc gacttttgta
      121 gggtgatttc acggtgccaa tggtagtaag ctccattttt cgcttgtagc tatccatttc
      181 tttttctccc ctatactatc acttagttta tggtgactaa taaaaattta tgctaggaac
      241 tatcataggt atcaattaga agatttccat taaaaatagc gttaaggatt ggtgataaca
      301 gatgtctctt gccttaagat ggtatccacc tgcctgggtg cagattaaaa ccagggaaaa
      361 aactatctac ctggacccct cctacttgaa gaaatatttt aaggaccatc ctaccgccag
      421 ggaatactcc aactggcctg atgaggtgga tggactacct gatgacctgg aaaaggctga
      481 cattatcttg gtaacccatc atcataagga ccatgtgaag aaggtaaccg tggacaggat
      541 aagtggccct gataccctga tcatggcacc cgggagggtg gagaaagccc tgggcaacag
      601 ggtaagggtg gtggcgcctg gtgatgaact ggaactggat gaagtgaaga tcaaggtggt
      661 gccagcatac aacacacccg aaggcagctc aacccgcaag ttccacaaac taggagaagg
      721 agtggggtac ctccttgaaa tagaaggcca cgttatctac cacgccgggg acacagacct
      781 catcccagag atggatggtc tgggaccagt tgacgtggca ttcctcccca ttggcggcac
      841 ctacaccatg aacgtagaag aagcagccca ggctgcctta aggatccagc cctccctggt
      901 ggttcccatc caccacctgc aagcagatcc ccaggactta caagtggaac tccggggtgg
      961 aggcatgaag gtaagggtcc tgaggacagg ggagctccta atgattgggg agtaagttta
     1021 taaccaaaaa aaggtctaac aaccattgta gttgtaaatt atttcttgtg taatacctaa
     1081 ctatgccatt tgattggggt tcacctgttt aactgggggt tttcttcatc actgatttca
     1141 aggagggcct ctactaccag ttttctcttc cttctatcct ggctttcaag ggccttcagg
     1201 agtccgggaa ctgcgagtct gtccagttcc accagggagt cctgggcgta ctggcgtaag
     1261 aggtcatccc cattctctaa taggtagatt agtggatcca ccgccctttt gtcattcaaa
     1321 cgtcccaggc tgcgggctgc cctccggcgg agagctgcat catcactgga aagagccctg
     1381 atcagtaggg gaagggcacg ggcatccttc aaagcaccta acttccgtgc ggcctccctc
     1441 ttctccagga gatctccccc ctccaggaga ctcatgaact tagccacctc atctccttca
     1501 cgggggttga aggcttcctc cacccggatg gcgaattgga agtccagatc agtgagacca
     1561 tccctgtcat gggtgaagat ggtgatctcc acctgccccc attccaaatc aatttcaggg
     1621 tggtgctgca ggtcctcggc cacctccccc acccggcggg tgaattcaag ggcactggcg
     1681 aagttaacga attggtacga tcccaccagg tgatggtttt ctatgatctt ccagttctcg
     1741 agttgagcag acctttcctc tatctcttcc tggtttaaaa gttctggtaa cataaaatct
     1801 tgcaccttca tggcttggta tagaataata ggatgttttt tcaaatcagg ctcttagcaa
     1861 actcttctgc atttttaagg tcttcggtgt ttggtcggcc tttattcatt cctccaaaca
     1921 gtctaaggaa actgttggtg ttaaagcctt tacattggaa ttcatctata accgtataac
     1981 ctttggatac aagcttttcc ctgagtttag aatgatcttt tgctgattta gatttcccgg
     2041 ttattcctgc agttgaaaaa atgaatgcat tcttattatt cacttccggt attacatcag
     2101 ctagttcaag tagagattcg tcgtgttttg cactgtaaat ccctgaacca aaccctacaa
     2161 ggtcatactc ttgaagttca tcaggattaa tttgatccgg catttttatt tctgcattaa
     2221 gaacttctgc cattacttta gctatttttt ctgtattatg gtggtggtat gagtacaaaa
     2281 ctaatagaga tttaccacga cccttgaacc tattgttctc cattattatc cctctaatct
     2341 aattaaccat atccaacaat tagatcccca attaataaaa tactgagcta atatgaatgt
     2401 ccagcatcat gtcagccagg atttatttta ccaaatggta atttaattct caactcctct
     2461 ttgcattatg tttttctaag cttaactgaa tcacagatgg attgaatcct ttaactataa
     2521 gccacactgc aaataccatt tcctgcacat gtatcggcac tcccaataat gcataggggg
     2581 aggctagcat gaaaagaata tcattcccaa aatcaaagcc tattccaacg ccaaaaatac
     2641 caagcaatcc atataacact gctaatgcgg ctccaattaa tccccaagct gataaccacc
     2701 gtggaactag tttggattgg tataacacat aattaagggc catgcttcct aacccaaata
     2761 agattaaaca tcccaataaa atagcccaac tttgtagtgc caagagtaag gtgcctaatg
     2821 cagggtaaaa tgaagcatca actgctcctg cggcgtattt ctggcttacg gttaacatta
     2881 ccagaagact catagcactg agaacataga gaacgttctc gaatgttctc aaaacaacat
     2941 atcccagggc taagctttca atatacttcc taagaatggg gaataacata aaggaagtgg
     3001 caaccgctga aatagcagca attaaaaaaa acaatacccc tactatcaat ccacttccat
     3061 gtgcagaaat gcctgtaaga taattgggag cttctagaat cgaacctata aagaccgagc
     3121 ctaaaatgga cgctaccgtg gcacttataa ataatactcc caccgttact ccggtctttc
     3181 tatatggatc cacatttttc aactcctatc ccttttgaag tttaaaataa aaaaaataaa
     3241 tccagaatat tgttattcca gatttaattt gatggagcca attctagggg ccaggtagtt
     3301 gtacagaagc gcaattaatg cggtttctat gaagtacact acgaagttgg atatgaattc
     3361 tccatagttc ttggacagga taccagatat aagccctaac aaggtgaaga caagggccac
     3421 tgatagggcg gtgggtagta cgggtatatg tttaagttca tgtaggcttc cagttatttg
     3481 accgaattct aactgtattt ttgctaccct actgaccagg tagttgtaga atagtgcaaa
     3541 taaggcgttc cagatgaatc caaacacgaa tgccattata ggcagcccga ttattaaaac
     3601 cagcgagact attgcctctg ctactccaat tcccggccca gtgggtatgg ccactccgga
     3661 tgcagttaaa ttggctgtta tgctggctgc ttcgggcatg gtgcccatgg caccaatcaa
     3721 tgagaaaagg ggtgaaatca ccgctgccag cactaatccc aggataaaag cccatactgc
     3781 cccgatagct gacaggatca gggaaaatgg aaccactgga atattttcta cttcattacc
     3841 caccagttct aacttcacac cacccaatct ggggactaat acgttgtaga gcatcaccga
     3901 gaagaaactc attactatag tgctgaaaaa cgcccctatg ggaaggagca caatcagggg
     3961 tattcctact ccagttacca ggttaaagtg tcctagctct gggattaccc ctgcaacttg
     4021 tacaatggtg agtccaatta acatcactac tgcagcaaca aatcccaaaa ctgcatatat
     4081 cgaagctgtc atcctagtaa agggtgccag cttaatccct ttaatttctt taatattcat
     4141 catcctaatt tacctcccat ctgaattaca aatccagatg atactttttt tataattcac
     4201 aattttaatc aactattttc attttcatga atcattgact acttaattct tgtttaaact
     4261 cttaaaagta gttattccaa attttcctca cattactgtg ggatttccct ccaaattcaa
     4321 tccaaaagtt tggagaaggc ttgttcataa aaataaaaaa aacgttttag ggaactcttt
     4381 aataaaaaat tcttaataac aaactgggta tgaaaagaat attatggttt attccctggt
     4441 tatcatcaga agtaacgcta atgctgtgaa ttcaagtcct aaaaatatta atagaataac
     4501 agagtttagc agcgattctg gaacgttaaa tatgaaatat actgaaaata aaatgttttg
     4561 gattaaaaaa agtgatgtga atagaaccaa ggtggtggtg aatttggact ttactttacg
     4621 gtaccttccc aggtacacat agagcatacc tactaaaaga cagatatttg ccacactcgt
     4681 tattaaagcc gaatacttaa ccagcaacaa caaagtaggt aacataattg gcattcccgt
     4741 gaaattttcc agcaacattt tttttacctt cgcttactaa ttatttatcc tcattttcct
     4801 taaattcttc ccagatttct aagaaggtat cataaacact ttccatctca tcagaaagaa
     4861 aatataatgc accatatgtt tctccagttg attttactaa attattttcc tttaaaacat
     4921 ctatatggtg tcttatagtt ttgtagtcaa gtgaaagttt ctgtgcaagt ttgttagcat
     4981 tatatggcct attgttgagt tcaagtatta tacgggcacg gttttctccg cctttactcc
     5041 cggctattaa ccaccacaaa aatactttgt tcataggaac tcctgtaaat tcatgatcaa
     5101 agaattataa gctctattaa tatctttcaa attaactcga taataaattt attcatactt
     5161 aaatcttctc tgtgctaaac cggttcaata agactctttc ttgaagcttc tgggcaactt
     5221 cataggtgtc gccagtgttg gaagatggaa taatagtgtc ttcgtgaaat ggaagagact
     5281 atcaactctt acctgtccgc aatgtaaaac gtgaattttt ataatcattt tctgtcgtct
     5341 attcgtttgg ttgtggttcg tcctataata gccactaaca gttcgttgtt tattttatgg
     5401 aatggaacat gatgggttta catttgcccg caggggacga aatgaattaa acacctattt
     5461 aaacttttca aaacacaata tttcatttaa ctcctttctc ggtctggggt caggattttc
     5521 atcggcatag cccagtgtaa acattgctgg cacccttatt tcatccggta tatccaagac
     5581 tttttttact gcttcttcat catatgcgcc caaccagcag gttcccagtc ccagttcggt
     5641 ggcttctaat atcatgaaag aaacagcgat ggatagatcc acagtgtaag caagttgacc
     5701 gcagggcatc agtctttctg attctgtgga gcatcccaca atggtggctg gtgcctggcc
     5761 cacgaatttc tgtccattag cagcttccac caatttatcc cgggtttcct ggtctttaac
     5821 aactataaat ttccattctt gcctgtttgc ggcggatggt gcgattctgg cagcttctaa
     5881 tattttttcg agtttctcat cctcaactgc tgtggtttta taatttc
//
LOCUS       DAOQVW010000030         5829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_15146_length_5829_cov_3.834430, whole genome shotgun sequence.
ACCESSION   DAOQVW010000030 DAOQVW010000000
VERSION     DAOQVW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15146_length_5829_cov_3.834430"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..911
                     /gene="hcp"
                     /locus_tag="PKK85_01195"
                     /pseudo
     CDS             1..911
                     /gene="hcp"
                     /locus_tag="PKK85_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265292.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
     gene            971..1309
                     /locus_tag="PKK85_01200"
     CDS             971..1309
                     /locus_tag="PKK85_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS24674.1"
                     /translation="MKIVKTKEMPIVDTPHKVDARKLYDTEHAVTVHITLQPGEKLIR
                     HITPVDVFFYVLEGTGIVEVGDEKQEVSKDTLIDSPARIPHCWYNESEGVLRFLVVKV
                     PKPLEETKLL"
     gene            1324..1515
                     /locus_tag="PKK85_01205"
     CDS             1324..1515
                     /locus_tag="PKK85_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24675.1"
                     /translation="MSDEDFDERAFEQALKGKMGWKCSCSLEIVDKKSELKEVTCKGC
                     GKTFKTNRDTDYCFRCDKH"
     gene            1560..2321
                     /locus_tag="PKK85_01210"
     CDS             1560..2321
                     /locus_tag="PKK85_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNS24676.1"
                     /translation="MAKYQEQNSHSYEMEIFSTSEGLQVIKSPLRVKILSILREKDLS
                     FDEIVKLSGRAKSTVSGHLKELSQEGIIGSRPHPTDARKKIFFIKSGYVGKLHRRNME
                     EDLKEHINRYIKSENNPFEFYRLIFHTIRVSLMNQGVNIDPILHDAGLSVGEVLYDQL
                     KSPTEEQFLQNIVRFWDTHNLGKLEVKNTKPLIINVKDCFECSGLPQLNRPACAFDTG
                     ILEAIFSRYYEKEAKVQEVECHAMGSGYCSFVIEI"
     gene            complement(2337..3170)
                     /locus_tag="PKK85_01215"
     CDS             complement(2337..3170)
                     /locus_tag="PKK85_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNS24677.1"
                     /translation="MKAKAYFGPAGNPIGFTGPTVEVCDYIHSIGLDAYEYQATYGVR
                     IKKQSARELGVNAQENGVMISMHAPYYINLSSPKEDVLERSVQRLVQSARAAEWMDAY
                     RIVFHPGFYTSYSPEEALKRCKNTIEALLEKLDGLGVTKFTFGPETTGKRSQLGSLEE
                     IIDICQSFEHFQPTVDFAHVHARNQGCLKDVDDYHRIFTKLEDGLGINILHSHFTKIE
                     YTGAGERRHHVLMEEKYGPPLEPLLESILECGWLVTVICESPLLEKDALLMKAMYESM
                     P"
     gene            complement(3327..4238)
                     /gene="trxB"
                     /locus_tag="PKK85_01220"
     CDS             complement(3327..4238)
                     /gene="trxB"
                     /locus_tag="PKK85_01220"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNS24678.1"
                     /translation="MGVYDLVIVGAGPAGLTAAIYAGRQGMKAIILEMMIGSGSGYMV
                     PAMENYPGFEKISGKNLLEIIKKQAEEHVIIKNMEEVKDITVLNNKNLEIITSKGEYR
                     SRAVIISTGSRHRRLKVPGEIEFIGRGVCYCATCDGPLYKGKDVMMIGGGNAAVQEAL
                     YLESMGCKVTIVHRRDELRAEKYLQEKLKEKNIPIIWDSVVEEIKGDAAVDEVVLYNR
                     KEDVHVVVETSGVFIAIGEEPLNEVAKSAGVELDGKGYIKVDKFQRTSIPGLYAAGDI
                     TGGIKQWVVACSEGAVAALFAHKDNMD"
     gene            complement(4245..>5829)
                     /gene="gatE"
                     /locus_tag="PKK85_01225"
     CDS             complement(4245..>5829)
                     /gene="gatE"
                     /locus_tag="PKK85_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HNS24679.1"
                     /translation="LVIASLLNMNVVDEFHTMRKQVIDGSNTGGFQRTGLVATDGYLN
                     TEYGKVVIENLCLEEDAARRMGQKRGMIEFRLDRLGIPLLEITTDPSMKHPQQVREVA
                     YQIGQVLRSTRVKRGLGTIRQDLNISITEGARVEIKGVQDLDLMPRMVENEVKRQINL
                     LKIRDELKRRGAQVSDEIHDLSHLFQETESKIIFNAINKKGKVLTIKLKGFRGLIGKE
                     VQPGRRFGTELAGYAKKMGVAGIFHTDELPAYGITSQEVERVNEFLDLDEYDAFILVA
                     DEEERSRNALLEVQRRAKAALIGVPEETRKSLDDANTEYLRPLPTASRMHVETDIPAQ
                     MISSELLEKVRSNLPELPAEKKERIIAEYGLSEDLAIQLVRQNRSDQFEELIGCCELD
                     PTIIASTLAYSLKELKREGLKVDELSTAVFMEIFKLVGHGKITKEAISPVIRKVAEES
                     VTPEDAARMLHLIMLSEKDVKNIVQKIVASQEEMVKERGSAATGPLMGMAMKELKGKA
                     DGKLVNKLLREEINKIRSR"
BASE COUNT         1598 a         1418 c         1157 g         1656 t
ORIGIN      
        1 actggacaca gcctcaaaaa tctggaagat ctattaaaac agactgaagg aaaaggaata
       61 aacgtttaca cccactcaga aatgttaccc gcccatggat accccggact gaaaaaatac
      121 acacatctag tgggtcaact cggcggaccc tggtttgacc agaaaaaaac cttcgccaag
      181 tatccagtgg ccattcttgg aacctctaat tgtgtacttt tacccaaaga cgaatacaaa
      241 gaaaggatgt tcaccagtgg agtggcccaa ctaccaggag tgcaacacat cgaagacaac
      301 gacttcaccc ccgtgattga aaaagccctg gaacttggtg atctagaaga agaaccacgt
      361 aaaacagttt taaccaccgg attcggtgca tccaccgtgc tttccctggc tcctaaaatt
      421 aaggaactgg tggaagccgg aaagatcaaa agattcttcc tggtgggcgg ctgtgactcc
      481 ccactacccc aagccaagta ctacaccgag ttcgtagcaa aactgcccca agacaccgtg
      541 gtactcacct tggcttgtgg aaagtaccga ttcaacgata tggatctggg agatattgaa
      601 ggggtgccca gactcatcga cttgggtcag tgtaacgatg ccatagtagc cattgatata
      661 gcagtggcac tcaccgaact attcggagtt gaacttaacg aactaccact aaccctgatc
      721 cttagttgga tggaacaaaa ggcagcagcc atcctatgga gtctcttggc cctgaacatc
      781 caaaacatgt acataggacc catactgccg ggttgggcta atgaagatat tataaatgtt
      841 ttggtggaaa actatggcct gacaccaatc gggacccaga agaagatatc aagaaaataa
      901 tggggtcata accccatttt tcttcttgaa aaaccaattc cttataataa gatctacatg
      961 agttgattgt atgaaaatcg ttaaaaccaa ggagatgccc atagtcgaca ctccccataa
     1021 ggtggatgca agaaaactat atgatacgga acatgccgtg accgtgcaca tcaccctaca
     1081 acctggtgaa aaactcatca ggcacataac cccggtggac gtttttttct acgtattaga
     1141 aggcacgggt atagtagaag taggtgatga gaaacaagaa gtatccaagg acaccctcat
     1201 tgacagccct gcccgtattc cgcactgctg gtacaatgaa agcgaagggg tcctccgatt
     1261 tttggtggtt aaagttccca aaccccttga agaaaccaaa ctgttgtgaa aaggaggaaa
     1321 gaaatgtctg atgaagattt tgatgaaaga gcatttgaac aagccctgaa aggaaaaatg
     1381 gggtggaaat gttcctgttc ccttgaaatt gtggataaaa aatctgaact caaagaagta
     1441 acttgtaaag gatgtggaaa aactttcaag accaaccggg acacggatta ttgttttaga
     1501 tgcgataaac actaactcat caaagctaac tgaccatgga gaaaagcagg gataataaga
     1561 tggccaaata tcaggaacaa aacagtcata gctatgaaat ggaaatattc tctacatcag
     1621 agggtttaca ggtaattaaa agtcctttac gggttaaaat attatctata cttcgagaaa
     1681 aggatctgag ctttgacgaa atcgtgaaac tatcgggccg ggctaaatca actgtttcag
     1741 gccatctcaa ggaactatca caggagggaa taatcggatc ccgaccacat cccaccgacg
     1801 cccggaaaaa gattttcttc atcaaatctg gctacgtggg aaagctccac cggcggaaca
     1861 tggaagaaga tcttaaagaa cacataaatc gttacattaa aagtgaaaac aatcccttcg
     1921 aattctaccg actcatattc cacaccatcc gggtttccct catgaaccag ggcgtgaata
     1981 tcgaccccat cctgcacgat gccggtttaa gtgtgggtga agtgctatac gaccaactaa
     2041 agagccccac tgaagaacaa tttttacaaa atatcgtccg attctgggat acccacaatc
     2101 tgggaaaact ggaagttaaa aacacgaaac ctttgattat caatgtaaaa gactgttttg
     2161 aatgcagtgg actgccccaa ctaaatcgac ccgcttgtgc tttcgataca ggaatattag
     2221 aagccatatt cagcagatac tatgaaaaag aggccaaggt ccaggaagta gaatgtcacg
     2281 ctatggggag tggttactgc tcattcgtga ttgaaattta atcaccttca ggatatttag
     2341 ggcattgatt catacatcgc tttcattaga agtgcatcct tctccagtaa gggactttca
     2401 cagataactg tgaccagcca accacattcc agaatagatt ctagcaatgg ctccagtggt
     2461 gggccatatt tttcttccat taaaacatga tgccgccttt ccccagcccc tgtgtactcg
     2521 attttagtaa agtgggagtg caagatatta atccctaatc catcctctaa ttttgtgaat
     2581 atacggtgat agtcatctac atcttttaaa catccctgat ttctggcatg tacatgggca
     2641 aagtctaccg ttggctgaaa atgttcaaac gactggcata tatctattat ttcttccagg
     2701 cttcctagtt gggatctctt accggtggtc tccggcccga aggtaaattt agtcacaccc
     2761 agaccatcca gtttttccaa caatgcttca atggtatttt tacatctttt aagcgcctct
     2821 tcaggggaat atgatgtgta aaagccgggg tggaatacga tacgataggc gtccatccac
     2881 tctgcagccc gggcagactg caccaagcgc tgcacagacc tttccagcac atcctccttg
     2941 ggtgaggaca agtttatgta gtaaggtgcg tgcatggaga tcattacccc attctcctgg
     3001 gcgttgactc ccaactcccg ggcagactgc ttcttaatac gcactccgta tgtggcttgg
     3061 tattcatagg catcaagccc tatactatgg atatagtcac aaacttccac tgtcggtcca
     3121 gtgaatccaa ttgggttgcc tgcaggccca aaatatgctt tagccttcat ttagatagtt
     3181 cttgattgat tttcttgagt ttgaagaaaa attcgggttc taagttatcc cgttttccgg
     3241 ataatatatg catcttgagg ggattattca ccatataccc atttagttct atttttttgc
     3301 caaatgtaat ccccaaatct tatacattaa tccatattat ccttgtgggc aaatagtgcc
     3361 gccaccgctc cctctgaaca ggccaccacc cactgtttta tgccgcctgt gatgtcacct
     3421 gcagcgtaga gcccggggat actggtgcgc tggaatttat ccaccttgat atatcctttt
     3481 ccatctaact ctaccccggc actttttgcc acctcattga gcggttcttc acctatggct
     3541 ataaatactc cggaagtttc caccaccaca tggacatctt ccttccgatt atatagtacc
     3601 acctcatcga cagctgcatc ccccttaatt tcctccacca cggagtccca aataatggga
     3661 atattttttt ctttcaactt ttcctgcaga tacttttcag cccttagctc atcccgccgg
     3721 tgcactatgg taactttaca tcccatgctc tctaggtaca aggcttcctg gactgctgcg
     3781 tttccacctc caatcatcat aacatctttt cccttgtaga ggggcccatc acaagtggca
     3841 cagtaacaaa ctccccttcc tatgaattct atttctccag gcactttgag tcttcgatga
     3901 cggctgcctg tggatatgat cacggcacga cttcgatatt cgcctttaga ggtgatgatc
     3961 tctaaattct tgttgtttaa aacggttata tccttaactt cttccatgtt ctttatgatt
     4021 acatgttctt cggcctgttt ttttatgatc tccagtaaat tctttccaga gatcttttcg
     4081 aaacctggat aattctccat ggctggtacc atgtaccctg atcctgaacc aatcatcatc
     4141 tccaggatga tggctttcat tccctgtcta ccggcataaa tagcggcagt taatcctgct
     4201 ggtcctgctc caacaataac cagatcatac acccccatac ttagtcacct actcctgatt
     4261 ttgttaattt cttccctgag gagtttgtta accagtttac catcagcttt acccttcaat
     4321 tccttcatgg ccattcccat caagggacct gtggctgctg atccccgttc cttaaccatc
     4381 tcttcctggg aagcaacgat cttctgaaca atatttttaa catccttttc tgatagcatt
     4441 attagatgaa gcattcttgc ggcatcttct ggagttacag attcttctgc cacttttctg
     4501 atgacggggc tgatggcttc tttagtaatt ttaccatgcc ccaccagttt aaaaatttcc
     4561 ataaaaactg cagttgacag ttcatccact tttaatcctt ctctttttag ttcttttaat
     4621 gaatatgcta aggttgaggc aattatagtt ggatccagtt cacaacatcc tattagttct
     4681 tcgaattggt ctgatctatt ctgtctaact aactggattg ccagatcttc actcaatcca
     4741 tattctgcga taattctttc cttcttctcg gcaggaagct ccggcaagtt tgatctgacc
     4801 ttttccaaca gttctgatga tatcatctgg gccgggatat cggtttcaac gtgcatgcga
     4861 ctagcagtgg gtaaaggccg gagatactcg gtgttagcat cgtccaggga cttacgagtt
     4921 tcttcaggaa cccctataag ggcagctttg gctcttcttt gtacctccag gagtgcgttt
     4981 ctggatcttt cttcttcatc tgccactagg ataaatgcat catattcatc tagatccagg
     5041 aattcattaa ctctttccac ttcctgggaa gtgatgccgt aggctggtag ttcgtcggtg
     5101 tggaatatac cggccacacc catttttttg gcatatcccg ccagttccgt gccgaatctc
     5161 cggccaggct ggacttcttt accaattaaa cccctaaaac ctttaagttt tatggttaag
     5221 acttttccct tcttattgat tgcattgaaa ataattttgg attctgtctc ctggaacagg
     5281 tgactcaaat catgtatttc atcagatact tgtgccccac gtcgtttaag ttcatcacga
     5341 atttttaaaa gattaatttg ccttttaact tcgttctcca ccatacgagg catcagatcc
     5401 aggtcctgga cacctttaat ctctacccta gccccttcag tgatggaaat attaagatcc
     5461 tggcgtatag ttcccaatcc ccttttcacc ctggtgctgc gcagcacctg gccgatctgg
     5521 tatgctactt cccgcacctg ctggggatgt ttcatggagg ggtcggtagt gatctccaat
     5581 agagggattc ccagccggtc caggcggaat tctatcatgc cccttttctg ccccatacgt
     5641 cgagcagcat cttcttctag gcagaggttc tctataacca ccttcccata ttcagtgttc
     5701 aggtagccat cagtggctac taaaccagtt ctctgaaatc ctccagtgtt actaccatca
     5761 ataacctgtt tgcgcatggt gtggaactca tccaccacgt tcatatttaa aagtgatgct
     5821 atgaccagt
//
LOCUS       DAOQVW010000031         5652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_15726_length_5652_cov_3.767375, whole genome shotgun sequence.
ACCESSION   DAOQVW010000031 DAOQVW010000000
VERSION     DAOQVW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15726_length_5652_cov_3.767375"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKK85_01230"
     CDS             <1..603
                     /locus_tag="PKK85_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNS24680.1"
                     /translation="AVAPIGGKEPLLGTNPIAIGIPSNVHYVSVDMATSAAARGKIME
                     AMRRGEEIPENVALDAEGNPTIDPCAAIKGSILPFGAHKGYALAFMIEIMSGPLVGAA
                     YGKYVTGTANPEVECTKGDLLSVIDPSCFVDLDQFKSEVDDFIAEVKATPNVFIPGDM
                     EIRNIRKFKKDGVSIDKKLEIQLKDLCQEVELRWDDVVEK"
     gene            678..1169
                     /gene="comD"
                     /locus_tag="PKK85_01235"
     CDS             678..1169
                     /gene="comD"
                     /locus_tag="PKK85_01235"
                     /EC_number="4.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase subunit alpha"
                     /protein_id="HNS24681.1"
                     /translation="MNSSQAVYQGIKKAGVDFVVSLPCVNLGRLMELVECDPEIMHIP
                     VTREEEGFGVCAGAFLAGKKPAILMQNSGLGNSVNVLASLYQLYRIPILMIISHRGTA
                     GEFMSAQVPMGNATPRVLDALKVVYLNPQTPDDALKLIPEAWILSEAGEAPMGILLDI
                     TFW"
     gene            1186..1731
                     /gene="comE"
                     /locus_tag="PKK85_01240"
     CDS             1186..1731
                     /gene="comE"
                     /locus_tag="PKK85_01240"
                     /EC_number="4.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase subunit beta"
                     /protein_id="HNS24682.1"
                     /translation="MQRIEAIRKICQYLGDELVICNIGFPSRELYQVKDSPTHFYMLG
                     SMGMASSIGLGLSLAQDRKVVVLDGDGSVLMNLGSLVTIYNQNPKNLVLVVLDNECYG
                     STGSQCTYSSTTDLKKIAESIGFQTFFFKDEIEFTKVLETDGPVFVHVKVLPGNADVP
                     VIPLEPEDIKKRFMEEVQGKK"
     gene            complement(1749..2318)
                     /locus_tag="PKK85_01245"
     CDS             complement(1749..2318)
                     /locus_tag="PKK85_01245"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNS24683.1"
                     /translation="MKKSIGAKTIVHPTPVFVVGSYDKEGKPNVMTAAWGGICSSVPP
                     CLSVSLREATYTHRNILEHQAFTVSIPTENYVKEADFFGITSGREVDKFQATGLTPVR
                     SAVVDAPYVEEFPFILECKLLKSVEIGGHTQFIGEIMDIKVEEDVVNADQPFVEEIKP
                     LMYSPDNIAYYGVGNFLGKAFSMGKLIKK"
     gene            complement(2366..2875)
                     /locus_tag="PKK85_01250"
     CDS             complement(2366..2875)
                     /locus_tag="PKK85_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNS24684.1"
                     /translation="MDFQKLIENRYSVRKYRNQDVEEDKLQKILDAARSAPTAANKQP
                     FKFIVIKTQDKKEDLKHIYHTDWFSEAPLIICACTIRSEAWTRRDGRCYVDVDVAIAM
                     DHLILAATNLGLGTCWVAAFDSHAARALLKLPPGVEPLLFTPLGYPAHEYQPTPRKEL
                     KELVRYGHW"
     gene            complement(2905..4053)
                     /locus_tag="PKK85_01255"
     CDS             complement(2905..4053)
                     /locus_tag="PKK85_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNS24685.1"
                     /translation="MLYHELGSTGEKVSILGFGCMRLPTRGHHDKIDEVKSSQLLDHA
                     LENGVNYLDTAYPYHGISNSEGGNSEVFIGEYLHQRDMHSQIHLATKLPTWLLQEPVD
                     MDRFLNQQLKRLKTDQIDFYLLHSLKEKNWFRLEDLGILDFLDSAVADGRIKYTGFSS
                     HDGTAFFKEVVDSYQWDMCQLQYNYLDEQIQVGKEGLEYAAEKGIGTVIMEPLKGGAL
                     ANYVPPEVKKLWEGFNRTPAEWAFRYLWNIPQIHVVLSGMNTMEQLMENLKIAEEGHP
                     DSLSPQENEVMQQVKQAYKDKIAVECSACGYCMPCPSGINIPQCLSYLNQAAMLNDIS
                     NIKSQYYFMLKDTEHASKCLECGLCEEMCSQKLPIREKLKEVKETLGD"
     gene            complement(4132..4419)
                     /gene="hisE"
                     /locus_tag="PKK85_01260"
     CDS             complement(4132..4419)
                     /gene="hisE"
                     /locus_tag="PKK85_01260"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNS24686.1"
                     /translation="MKDKVIRDIYQVLENRRDHPVESYTSRIMQDSQKKAEDKILEKI
                     GEEASEVIIASKNDENLVQESADLIFHTLLLLVYKGVELDELLDEFERRRG"
     gene            complement(4421..5233)
                     /locus_tag="PKK85_01265"
     CDS             complement(4421..5233)
                     /locus_tag="PKK85_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS24687.1"
                     /translation="MSSSALVKDYMTRKVITVTPDTSNEEVIELMKATGHDGFPVKTN
                     GKVTGMVTAFDLVLKPWLNSVSEIMSTDVVVADQEMSISDASRVMFRMGISRLPVMDG
                     EGHLVGIITNTDIVRSHIERSTPMKVKYFQKTLEQLYDIKTSLIHKTVPIDKLRPTQD
                     RIYADELQGRTYELQMGLAEPTIVVETGDRFVLVDGHHRTVAARKLGYQEIDSYVIKL
                     DRDLKLGMEKTADKNGIFSLDDVQIIDDAQHPLIAITTSLRQESDNKDKNED"
     gene            complement(5230..5478)
                     /locus_tag="PKK85_01270"
     CDS             complement(5230..5478)
                     /locus_tag="PKK85_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HNS24688.1"
                     /translation="MAKHVLNMMLWHPEKKIKNCTVIYIHRGAPGGLKKIGGDEIKSV
                     EGGFMILGDETKIPYHRVVRIECDGEIMWEKDLKERKK"
     gene            complement(5517..>5652)
                     /locus_tag="PKK85_01275"
     CDS             complement(5517..>5652)
                     /locus_tag="PKK85_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24689.1"
                     /translation="VNDYYKGQKKALNYLVGQVMKLTRGKADPSHTNQLIRDELEVKD
                     "
BASE COUNT         1488 a         1303 c         1188 g         1673 t
ORIGIN      
        1 gccgtggccc ccatcggagg taaagaacca ttacttggta ccaatcctat agccattggc
       61 ataccttcca acgttcatta tgtttcagtg gatatggcca cctccgctgc agcaagagga
      121 aagatcatgg aagctatgcg cagaggagag gaaatacccg agaatgtagc tctggatgct
      181 gaaggtaacc ccacaattga cccctgtgcg gccataaaag gatccatact tcccttcgga
      241 gcccataaag gctatgccct ggcattcatg atcgaaataa tgtccggccc cctggtgggc
      301 gctgcctatg gcaaatacgt gactggaact gccaaccccg aagtggagtg caccaaaggg
      361 gatcttctgt cagttataga tccctcctgc ttcgtggatc tggatcagtt caaatcagaa
      421 gtggatgatt tcatagccga agtaaaggcc acccccaacg tgttcatacc aggtgacatg
      481 gaaatccgga acattagaaa attcaaaaaa gatggagttt ccattgacaa gaagctagaa
      541 atccagttaa aagatttgtg ccaggaagtt gaacttaggt gggacgatgt ggtagaaaaa
      601 tgatttttaa catctagcaa tggaatccaa ctcctaactt caagatcagg atgaattaag
      661 gacatcaagg tgttgtagtg aacagtagcc aagccgtgta tcaaggtatt aaaaaagcag
      721 gtgtagactt tgtggttagt ttgccctgtg tcaacctcgg tagattaatg gaactggtgg
      781 agtgcgaccc agaaattatg cacatccctg ttacacggga ggaagaaggc ttcggagtat
      841 gtgcaggggc atttctagcc gggaaaaaac cggcaatact catgcaaaac tcaggactgg
      901 gcaattcagt aaatgtactg gcatctcttt atcaactata ccgaattccc attttgatga
      961 taataagtca tcgtggcact gcaggggaat tcatgagtgc ccaggtcccc atgggaaacg
     1021 ccactccccg agtgttagat gccttgaagg tagtttatct taacccccaa accccagatg
     1081 atgctttgaa acttataccc gaagcctgga ttctgtcaga agctggtgaa gcccccatgg
     1141 ggatactttt ggatattaca ttctggtgaa cttagaaggt ggaagatgca gagaatagaa
     1201 gcaatccgta aaatttgcca atatttgggg gatgaactgg tgatttgcaa catcggattc
     1261 ccatcccggg agttatatca ggttaaagat tctcccaccc acttttacat gttaggatcc
     1321 atgggcatgg cctcttccat tggcctggga ttatctctgg cccaggaccg taaggttgtg
     1381 gtcttggatg gggatggatc agttttgatg aacctaggaa gcctggttac catatacaat
     1441 cagaatccta agaatctggt tctggtagtc ctggataacg agtgttacgg ctccactggt
     1501 agtcagtgca cctactcatc caccactgat cttaagaaga tagcagaatc cataggcttc
     1561 caaacttttt tcttcaagga tgaaatcgag tttacaaagg ttttagaaac agatggacca
     1621 gtattcgtcc acgttaaagt tcttccagga aatgcagacg ttcctgtaat tcctctggag
     1681 ccggaagata ttaaaaagag atttatggaa gaggttcagg gaaaaaagta gcagggattt
     1741 tcatcccttt acttttttat tagttttccc atagagaagg cctttcccag aaaatttcca
     1801 actccatagt aagcgatatt atcaggactg tacatcaatg gtttgatttc ttcaacgaat
     1861 ggctgatcag cgttaactac atcctcctcc actttaatat ccatgatttc acctataaat
     1921 tgtgtgtgcc ccccgatttc aacgctttta agtaatttgc actccaaaat gaagggaaac
     1981 tcctcaacat atggtgcatc caccactgca ctcctcactg gagtcagtcc cgtggcctgg
     2041 aacttgtcta cttctctgcc tgatgttatt ccaaaaaaat cggcttcttt tacataattc
     2101 tctgttggga tgctaactgt gaaggcctgg tgttccagta tattacgatg ggtgtaggtt
     2161 gcttcccgca gggatactga aagacatggt ggtacagatg aacatatacc accccatgct
     2221 gctgtcataa catttggttt accttcctta tcgtaagatc ccaccacaaa gactggggtg
     2281 ggatgcacaa ttgttttagc accgattgat tttttcattt tcttatcttc cttttaaata
     2341 ttgttcatgg aaaaaaactg aatctttacc agtgcccata acgcaccaac tcctttaact
     2401 ccttccgggg tgtgggttgg tattcatggg ccgggtatcc aaggggcgtg aataataatg
     2461 gttccacccc gggtggtagt tttaatagtg cccgcgcagc atgtgagtca aaggcagcta
     2521 cccaacaagt gcccagaccc agattcgttg ccgcgagtat gaggtggtcc attgctatgg
     2581 caacatccac atccacatag caacgcccat cacgacgcgt ccatgcctca gaacgaattg
     2641 tgcaagcaca tataatcagg ggggcctcgg aaaaccagtc cgtatggtag atgtgtttta
     2701 aatcctcctt tttgtcctgg gttttaatca caatgaattt gaatggctgt ttatttgccg
     2761 cggtcggggc tgaacgtgcc gcatccaaga ttttttgcag tttatcctct tcaacatctt
     2821 gattccgata ttttcggacg ctgtaacggt tttcaatcaa tttctgaaaa tccatacaat
     2881 atcaactcca aatatccagt tcatttaatc ccccaaggtc tcctttactt ctttcagttt
     2941 ctctcggata ggaagtttct gggaacacat ctcctcgcac agtccacatt ccagacattt
     3001 agaggcatgt tcagtgtctt ttaacatgaa atagtactgt gacttgatat tgctaatgtc
     3061 gttaagcata gctgcctggt ttaggtaact taagcactgt ggaatattta tacctgaagg
     3121 acatggcatg cagtaaccac aggcactgca ttccacagct atcttgtcct tataggcttg
     3181 tttaacttgt tgcatcacct cattttcctg gggggatagt gaatcggggt ggccttcttc
     3241 agctatttta aggttttcca tgagttgctc catggtgttc attccactta aaaccacatg
     3301 aatctgcggg atattccaga gataacgaaa cgcccattcg gcgggtgttc gattaaatcc
     3361 ttcccacaac ttcttcacct cgggaggaac ataattggcc agagccccac cttttaaagg
     3421 ttccataata acggtaccaa tacctttctc agctgcgtac tccaggcctt ccttaccgac
     3481 ttgtatctgt tcatctagat agttatactg taactggcac atatcccact gatatgagtc
     3541 caccacttct ttaaagaatg cagttccatc gtgacttgag aatcctgtgt attttattct
     3601 gccgtcagca actgccgaat ctaaaaaatc tagaatcccc agatcctcca ggcgaaacca
     3661 gtttttctct ttcaaggagt gcaggaggta gaaatctatt tggtcagttt ttaagcgttt
     3721 tagttgctgg ttaagaaatc tatccatgtc tactggctct tgcaacagcc aagtggggag
     3781 tttagtggcc agatgaattt ggctatgcat atccctttgg tgcaggtact ctcctatgaa
     3841 gacttcactg ttaccccctt ccgagttact gatgccatgg tagggatagg ctgtgtccag
     3901 atagttaacc ccattttcca gggcatgatc aaggagttgg gaagatttta cctcatcgat
     3961 cttatcatga tggcccctag ttgggagtcg catgcatcca aatcccagta tggagacctt
     4021 ctccccggtg gagcccagtt cgtggtatag catggtgtat cctaaatttt tatcattgga
     4081 gtaatcccat taaaaatcat ggccagcagt agagccattt taaaaaaaaa tttaacccct
     4141 ccttctctca aattcatcca gtaactcatc aagttccact cccttgtaga ccagaagaag
     4201 gagagtgtgg aatatcagat ctgctgattc ttgaaccaga ttttcatcat ttttagaagc
     4261 tattatcacc tcggaggctt cttctccgat tttttccagg atcttgtctt ccgccttctt
     4321 ctggctgtcc tgcatgatac gggaggtgta agattcaact ggatgatcac gacggttctc
     4381 cagtacctgg taaatatccc taataacctt atccttcact ttaatcctcg tttttatcct
     4441 tattatctga ttcctgacgt aaactagtgg ttattgctat taatggatgc tgggcatcat
     4501 ctattatttg tacatcatca agtgagaata tgccattttt atctgccgtc ttctccatgc
     4561 ctagtttaag atcacgatcc agttttatca cgtaggaatc tatttcttgg tatcctaatt
     4621 tccgggcagc aacagtgcga tgatgcccat ccaccagcac gaaacgatct ccagtttcca
     4681 ccacgatagt aggctcagcc aagcccattt gaagctcgta agtacgtcct tgaagttcat
     4741 cagcataaat acggtcctgt gttggcctta acttatcgat gggcacggtt ttgtgtatta
     4801 atgaagtttt gatgtcgtaa agttgttcca gggttttctg gaaatatttt accttcatgg
     4861 gggtggatct ttctatatgg gatcgtacaa tatcggtgtt ggtaatgata cccactaggt
     4921 gtccctctcc atccatcact ggtaagcgtg atatgcccat gcgaaacatg actcgggagg
     4981 catcgctaat ggacatctcc tggtctgcca ccaccacatc ggtagacatg atctcactca
     5041 cactatttaa ccaaggtttt aataccaagt caaaggcagt taccatgcca gtaaccttcc
     5101 cattagtttt aactggaaat ccatcgtgac cagtggcttt cataagttca atcacttctt
     5161 cgttggaagt atccggtgtg acggtgatga ccttcctggt catgtagtcc ttcaccaggg
     5221 cggaactact catttttttc tctccttaag atctttttcc cacattattt ccccatcaca
     5281 ttcaattcgt actactcgat gataggggat cttggtttca tctcccaata tcataaagcc
     5341 accttccact gatttaatct cgtcaccccc aatctttttc aggcctccag gggctccgcg
     5401 atgtatataa ataactgtgc aatttttaat ctttttctca ggatgccaga gcatcatatt
     5461 caaaacatgc ttagctatga aacctccccc aataccgtct ggagaaaaaa aatagattag
     5521 tccttgacct ccaattcatc tctaattaac tggttggtgt gggaaggatc tgcctttcca
     5581 cgggttaatt tcattacttg gcccactaaa tagtttaagg ccttcttttg gcctttgtaa
     5641 tagtcgttaa ct
//
LOCUS       DAOQVW010000032         5600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_15922_length_5600_cov_2.801984, whole genome shotgun sequence.
ACCESSION   DAOQVW010000032 DAOQVW010000000
VERSION     DAOQVW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15922_length_5600_cov_2.801984"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1996
                     /locus_tag="PKK85_01280"
     CDS             <1..1996
                     /locus_tag="PKK85_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNS24690.1"
                     /translation="VEAGVTDIFVKVSRLVDYQTTLDSLAGVKEMVSGRGIRIHAWVT
                     CFLDADGKWVDPLGTYSYTVQVPFQQTVKVAYQEAYKSWYKSYYKSWYKSWYKSWYKS
                     WYKSGSKWKYKWRYTWKYTWKYTWKYVLKYGWNYRTAYKYVTTTSYRTETRYGTDTGL
                     NDELVQFTSKLASSKLVDGIHLDYVRYPGTAYKYTGGTEAITQFVARVNTAVKSVNPS
                     TALSASLMPEGSVNAYYYGQDYSKLATYLDFLVPMVYKGNYKADTDWIGRMTNYIVSQ
                     ANGKPVLVGLQTYRSDNDLSRLPAAELEQDINKALEKGASGYVLFRYGLVDLSFLGGT
                     SYSPPETGSSTNPPTPTPEPSPVYFTLDQIGDAAGRVKNFILENQRLPNYVLISSREV
                     SMPQFLYLVSTATIHANNGIRNSIALVNAGDPLEPSEDVKSGNINCPELVDIAQSIKN
                     FMEASNRAPNYAASSLGKMKLETLLSLYSRVLDFYCTNQRMPNYATVNPWISPVQSSG
                     PVTSPGDLQQYLAATSNCQSNDPQIIALAQSITTNCASSYEMGLAIFNWVRDQLDYSW
                     YYNTQKGAVNAMLTRTANCCDMSHLIVALCRAVNIPARYQHGTCNFNSGSYGHVWAEL
                     YVNDNWISADATSNANSFGIIKNWNTNNYIWKGTYAQLPF"
     gene            2090..2314
                     /locus_tag="PKK85_01285"
     CDS             2090..2314
                     /locus_tag="PKK85_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24691.1"
                     /translation="MCEYRKECDCKHRHYHHRRYPGRGVSSIVQRSSETVEVPAELLE
                     VAEIKEGDYLKIRVHKVIKPSEWRKRHHPD"
     gene            complement(2456..2602)
                     /locus_tag="PKK85_01290"
     CDS             complement(2456..2602)
                     /locus_tag="PKK85_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24692.1"
                     /translation="MEAMDQNDIEDFVANFKGMLWDELEDYICDMSREDLQALIMGLK
                     KRFG"
     gene            2807..3556
                     /locus_tag="PKK85_01295"
     CDS             2807..3556
                     /locus_tag="PKK85_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24693.1"
                     /translation="MASYKVRSFQDCAYFTKTRSFLKLYPVLEGLKHSRGRFIHVIGA
                     PGTGKSANIYSTLALLDLKVYEAFLFLDSVELTPDEVWDQFWATLHEDLGAESRDDVY
                     QEAAQYDVVLFADIFLDPEYLLEDKVGLGLWTEENGPKTVTFYTRVLWEYLRHRQDLK
                     NINIVTQTAWILKYQGVKYDLLTDFYILSWILVFILKIFFEVVRISYSEEEIIRIIRN
                     YHSHCSEEEIKNLINKYGCRPRFILEALKKA"
     gene            complement(3558..3830)
                     /locus_tag="PKK85_01300"
     CDS             complement(3558..3830)
                     /locus_tag="PKK85_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNS24694.1"
                     /translation="MKVIKKKGEKEAFKADKIKRTLQKATIGAGYSVDEKQAILDEVF
                     TNIMKKLDQKGEVKTQTIRGCLLTELDQCEPYIAKSWRNYERKYKK"
     gene            complement(3842..4585)
                     /locus_tag="PKK85_01305"
     CDS             complement(3842..4585)
                     /locus_tag="PKK85_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNS24695.1"
                     /translation="MDLDLVLNDALKYPYQALERVLILGVVPLGAMIISLVILVALSF
                     TGSAEALAIGGFLVMVLFFLSCLFVGGYYIRVMESTLTGGEEVPDFSRWGELLLNGLK
                     LLVVKLLYLLVPLLILVLCSWLVISTPDEHLVVVVLLSICLVLLSMLLSGLFLALSTA
                     HLASTRSMKEALRIGLVWKMISAIGWGDYLLWYLLMLVLVMVAVTLMALLSIVPLVGA
                     FLGWILIQPYLVILVARSVALAYLAADEV"
     gene            complement(4604..4981)
                     /locus_tag="PKK85_01310"
     CDS             complement(4604..4981)
                     /locus_tag="PKK85_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24696.1"
                     /translation="MTRKQFKKKSSQALKDMGPSILLLMELTAKQQKKTITTEEAETR
                     LDLIRKDVEEVFFRYEKINPPSTYQSLYLKILQLLSNLQEVVVSSQEYLSQDDEQLLE
                     ESQELLNRFREEYHTIRREVEST"
     gene            complement(5447..5584)
                     /locus_tag="PKK85_01315"
     CDS             complement(5447..5584)
                     /locus_tag="PKK85_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNS24697.1"
                     /translation="MACEALGCGTCAIGAYDQEYFDELLGLDGEEEFVIYAAPVGRVK
                     K"
BASE COUNT         1521 a         1395 c         1263 g         1421 t
ORIGIN      
        1 ggttgaggcc ggagttactg acatattcgt taaggtcagc cgactggtgg actatcagac
       61 taccctggat agcttggctg gagtcaaaga aatggtttca ggcagaggaa ttaggatcca
      121 tgcctgggtg acctgtttcc ttgatgcaga tggtaagtgg gttgaccccc taggtaccta
      181 cagttataca gtgcaggtac cattccagca aactgtcaaa gtggcatacc aagaagccta
      241 caagagttgg tacaagagct actacaaatc ctggtacaaa agctggtaca aatcctggta
      301 caaaagctgg tacaagtccg ggtctaaatg gaaatacaag tggagataca cctggaaata
      361 tacctggaag tacacctgga agtatgtgtt gaagtatggt tggaattacc gtactgctta
      421 taagtacgta actaccacgt cgtataggac tgaaacccgt tacgggacag acaccggtct
      481 caacgacgaa ttggtacaat ttacttctaa actcgccagt agtaaattag tagatggcat
      541 tcacctggat tatgtaagat atccaggcac agcctacaaa tataccggtg gaaccgaagc
      601 cataactcaa ttcgtggcaa gagtaaacac cgcagtcaaa tcagtgaatc ccagtactgc
      661 gctatccgca tccctcatgc cggagggttc ggttaatgcc tactactatg gacaggatta
      721 cagcaaacta gccacctacc tggactttct ggtacccatg gtttacaagg gaaattataa
      781 ggcggacacg gattggattg gtagaatgac caattacatc gtgagccagg caaacggaaa
      841 acctgtccta gtcggattgc agacttaccg ttcagataat gatctgagcc gattacctgc
      901 agcggaactg gaacaggaca tcaacaaggc acttgaaaag ggtgcttctg gttacgtatt
      961 attccgttac ggcttggtag atttatcatt cctgggtgga accagttaca gtccacctga
     1021 aactggtagc tccaccaatc cacctacacc caccccggag ccatcacctg tctacttcac
     1081 actggaccag atcggtgatg cagcgggtcg ggtgaagaac ttcatcctgg aaaaccagag
     1141 actacccaac tacgtgctaa tttcctccag ggaagtcagc atgccccagt tcctgtacct
     1201 agtaagcaca gctaccatcc atgccaacaa tggaatcaga aacagcatag ccctagtaaa
     1261 tgctggggat cctcttgaac cctcagaaga cgtgaagagt ggaaacataa actgccccga
     1321 actagtagac attgcccaga gtatcaaaaa tttcatggaa gccagcaacc gggcccctaa
     1381 ctacgctgca agctcactag gaaagatgaa gctcgagaca ctcctcagct tgtacagccg
     1441 ggttttagat ttctactgca ccaatcagag aatgccaaac tacgctacag ttaacccctg
     1501 gattagtcct gtacaatcat ccggaccagt gaccagcccc ggtgatctgc aacagtattt
     1561 agcagccacc agcaactgtc aatccaatga tccacagatc attgcattag cccagagcat
     1621 aaccaccaat tgcgcctctt cctatgagat gggattggcc atattcaact gggtccggga
     1681 ccagctggat tactcctggt actacaacac ccagaaaggt gcggttaatg ccatgttaac
     1741 ccgcactgcc aactgctgtg acatgtccca tttgatcgtg gccctgtgtc gggctgttaa
     1801 cataccagcc aggtaccagc atggaacctg taacttcaac agtggaagct atggacatgt
     1861 ttgggccgag ttgtacgtga atgataactg gatctccgca gatgcaacca gcaatgccaa
     1921 cagttttgga atcataaaaa actggaacac caacaactac atctggaaag gcacctacgc
     1981 acagttgcct ttctaaaaaa aaccccttct ttttttcttt ttttagaaac actttacacc
     2041 ttcgatactt tcaaatattc agttgaccaa ctagacttga ggtgaaaaga tgtgtgaata
     2101 cagaaaggaa tgtgactgca aacaccgcca ttatcatcac cgccgctacc caggccgtgg
     2161 tgtttcatcc atagtccagc gaagctctga aaccgtggaa gttcctgctg aactcctgga
     2221 agtggcggag ataaaggaag gagattatct gaagatccgt gtgcacaagg tcattaaacc
     2281 tagtgaatgg agaaaacgtc accacccgga ttagatccat ctccaactat ttttttttaa
     2341 ttcaaggtta ctgttacata aaaaaaatat tgggttcggc aagacaagcc cccccagcct
     2401 catcttccga accacggtat accaaacaat acgggaaaga cttaatggaa ccatatcatc
     2461 cgaatctctt cttaagcccc atgattaggg cctgcaaatc ctctcggctc atgtcgcata
     2521 tgtagtcttc taattcatcc cacaacattc ccttgaagtt ggccacgaag tcttcaatat
     2581 cattctggtc catagcttcc atttcccccc cagaggtgta gcactctatt tctccctatt
     2641 acatcttagt acatactgat aaataaatta tttggcgaaa aaattaaata gggacttatt
     2701 taataaaaag aggtgtccta gtattaaata gcctttcatc acataaattc ttcttactac
     2761 tcttgagaaa gaaaaacatt agaaccatgg gcagcaacat agttcaatgg ccagttacaa
     2821 ggttcggtcc ttccaggact gtgcatactt caccaaaacc cgttcctttc tgaaactcta
     2881 ccccgtgctg gagggtttga aacacagccg gggaaggttc atacacgtca ttggtgcgcc
     2941 tggtactggt aaatccgcta acatctacag taccctggct ctccttgatt tgaaggtcta
     3001 tgaggctttt ctcttcctgg acagtgtgga gctcaccccg gatgaagtct gggaccagtt
     3061 ctgggccacc ctccatgagg atctgggagc agaatcacgg gatgatgtct accaggaggc
     3121 tgcccagtac gatgtggtgc tttttgctga tatcttcctg gacccggagt atttactgga
     3181 ggataaggtg ggtcttggtt tgtggaccga ggaaaacggc cccaaaactg ttaccttcta
     3241 cacgcgtgtg ctgtgggagt atctgaggca ccgccaggat ctgaagaaca ttaacatcgt
     3301 cacccagacg gcctggatac tgaagtacca gggggtgaag tacgaccttc taactgactt
     3361 ctacatactc tcctggatac tggtcttcat tctaaagata ttctttgagg tggttcggat
     3421 atcctacagc gaggaggaaa tcatcaggat catcaggaac taccactccc actgcagtga
     3481 ggaggagatc aaaaatttga taaataaata tgggtgcagg ccccgcttca tactggaggc
     3541 cctaaaaaaa gcctagacta ttttttgtac ttgcgttcat agttccgcca ggacttggca
     3601 atgtagggtt cgcactggtc cagttcggtt agaaggcagc cccgtatagt ctgggttttt
     3661 acctcaccct tctggtccaa cttcttcatg atgttggtga acacctcatc cagaatagct
     3721 tgtttctcat ccacactgta tccagcgccg atggtggctt tctgcagagt tcttttaatt
     3781 ttatcggcct tgaaggcctc tttttctccc ttctttttta tcactttcat acttatcacc
     3841 tttatacttc atctgcggcc aggtaggcca gggccacgga acgggccacc agtatcacca
     3901 ggtagggctg gatcagtatc catcccagga aggcacctac caggggtacg atgctgagta
     3961 aggccatgag ggtcacagcc accataacca gtaccagcat cagcaggtac cacagtaggt
     4021 agtcacccca accaatggca gatatcattt tccagacaag gccgatgcga agcgcctcct
     4081 tcatgcttct ggttgatgcc aggtgggcag tggatagggc caggaacaat ccagatagta
     4141 acatgctaag taacaccagg cagatggata agagtacaac caccactaga tgttcatcgg
     4201 gagtggaaat aactagccag ctacacagta ccaggatcag cagggggacc aggaggtaga
     4261 gtaacttcac caccagtagt ttcagtccat tcaggagtaa ctccccccac cgggaaaaat
     4321 caggcacctc ctctccacca gtcagggtag actccataac ccgtatgtag tacccgccaa
     4381 caaagaggca ggataagaaa aagagcacca tgaccaggaa acctccaatg gccagggcct
     4441 ctgcactgcc tgtgaaggat agggccacta agattaccag ggaaataatc atagccccca
     4501 gggggaccac tcccagtatc agcaccctct ccagggcttg gtaggggtac ttcagggcat
     4561 cattcagcac aagatcaagg tccatggttt cactccatta tccctaggtt gattctacct
     4621 cacggcggat ggtatggtat tcctcccgga acctatttaa gagttcctgg gactcctcca
     4681 ggagttgttc atcatcctgg gatagatact cctggctgga taccaccacc tcctggaggt
     4741 tgcttagaag ctgcaggatc ttcaggtaca gggactggta ggtggagggt gggtttatct
     4801 tctcgtagcg gaagaacacc tcttccacat cctttcttat caggtccagt ctggtttcag
     4861 cttcctccgt tgtgatggtt tttttctgct gcttagcagt aagttccatc aaaagaagaa
     4921 tggatggacc catgtccttc agggcctggg aactcttctt tttgaattgc ttacgggtca
     4981 tacctaacat caaacttagt tcctcactgc atcatatcca gatgcttttt gtctacttcg
     5041 tactgtacct ggtgccattc ccccattttt taatactgat cctgggtctt ggatgcaacc
     5101 tgcaatgtac catttccagg taggtatcta ttcactgctg gtcttctttc atttttcttt
     5161 tattctcagt gcactaaccc agtcatatta tttttggggg aactttaata ggtaggcaca
     5221 tatgtcttgg ttcagatcga ttataatatt tttcctaaag tcactagggc cgatagattg
     5281 ggcgggtgta gttaaaagcc agtcacatac tggatagcct tctccagaat aagttgtaag
     5341 gggtgttaat tcccaatcca ggatcaggtg tggaaggata atatcttcca gagcagcccc
     5401 ggtgggggct gttgcataag gatcgttgcc cctagtttgt ggtcagttac tttttaaccc
     5461 tacccactgg tgcagcgtag atcacaaact cttcctcacc atccagtccc agtagttcat
     5521 cgaagtattc ctggtcgtag gcaccaatgg cacaggtacc acaacccagg gcttcacagg
     5581 ccaggtacag gttctggccc
//
LOCUS       DAOQVW010000033         5512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_16263_length_5512_cov_3.430273, whole genome shotgun sequence.
ACCESSION   DAOQVW010000033 DAOQVW010000000
VERSION     DAOQVW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16263_length_5512_cov_3.430273"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKK85_01320"
     CDS             complement(<1..232)
                     /locus_tag="PKK85_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03576 family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNS24698.1"
                     /translation="MLVNSSLDEIKRRETALHYIADLVRENGREKLYDLTGLAGGFPL
                     NPDDIDLLETYAGPALFQEDLDFWGRKHLGGEK"
     gene            complement(257..763)
                     /locus_tag="PKK85_01325"
     CDS             complement(257..763)
                     /locus_tag="PKK85_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00288 family NYN domain-containing protein"
                     /protein_id="HNS24699.1"
                     /translation="MRSFERLTSLKDYIPLKRSESGGKNIGLLVDGPNMLRKEFSLNL
                     DLVRDIIAEYGNMRVGKVLLNQYASDKLIEAIVNQGFTPIVVAGDTDVYMAVEAMELI
                     YNPNIDVIALMTRDADFLPIINKAKENGKETIVIGAEPGFSAALQNSADSAIVLTPED
                     SRKNHGRK"
     gene            complement(1099..1422)
                     /locus_tag="PKK85_01330"
     CDS             complement(1099..1422)
                     /locus_tag="PKK85_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24700.1"
                     /translation="MSDADSKVNKELQKRMKEFKAALKNEAQKQEFLDNLPLLIRIEV
                     FLPSTNPEEYVNGLYLYMGDAEGIVNAEYYFQDKDEVKICSLSGNELEVVKELFKDEF
                     DAEME"
     gene            complement(1757..2512)
                     /locus_tag="PKK85_01335"
     CDS             complement(1757..2512)
                     /locus_tag="PKK85_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24701.1"
                     /translation="MKQFLITPAAGKRLIAKALMEHPEIKKTLRSGTLAIIAGTTNGY
                     VAEEILKSMGQENDFSRKRFFRGIVLPPGAPVNPDGRYADDAGFPGDVIITDGVWEKG
                     MRIFDVVDSLKEGDIILKGANALNLDLKQAAIFIGHPQAGTIGAALPGVVGRRVRLIL
                     PVGLEKRVSSDLNELAVELNQPGLKGPRLLPVPGEVFTEIEAIKFLTGAKAKLVAAGG
                     VAGAEGSVWLAIPNNEKIEKVLNEVSTESPFSL"
     gene            3360..3746
                     /locus_tag="PKK85_01340"
     CDS             3360..3746
                     /locus_tag="PKK85_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24702.1"
                     /translation="MDKELYVKEAIQNRVDFVEPLKKTGYNMGELHALKWVLKLLNDE
                     ILVYDEETYWKLMKSEKDCPTCNAILLRVTGKYNGTYESYAPPNEYGRSLKEHLTSVK
                     EADGCMGDTCIEKDGEKIVMIYSENK"
     gene            4013..4636
                     /locus_tag="PKK85_01345"
     CDS             4013..4636
                     /locus_tag="PKK85_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS24703.1"
                     /translation="MPISDRKEREKEMRRKDIIDTAEKLIFENGYENITMADIAKGSE
                     LARSTLYLYFKNKKEIYLSISIRGTQILNKMFKEYYNRGETGLEKVKMLMIAFYKFYK
                     EYPGYYDVNWLSYPVAFNHDLPKMKEVKRMRMEGFSLFGKALQEGIEDRSIRSDIDPV
                     KTNLVLALSIQNVLNLPPTMELHMKNNNLTHKELIDYTMDLMLHSLE"
     gene            4656..5141
                     /locus_tag="PKK85_01350"
     CDS             4656..5141
                     /locus_tag="PKK85_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24704.1"
                     /translation="MQSKSKTKKKSFISKDTVNWKLYLILLGACIFGTLAVLPYTLTL
                     QGGLLENSTVPLYFILAAQIIQSIVLFSVTIFIGLLLSKKVGLELPILEGWLGGREVK
                     NYLKSVLGISIGLGILASIMIIGIDYLFSSTGLTSSIVMAQITPPRLARTPFIILWWN
                     K"
     gene            5146..5472
                     /locus_tag="PKK85_01355"
     CDS             5146..5472
                     /locus_tag="PKK85_01355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNS24705.1"
                     /translation="MLLRLFLMTLITWIIFKIRKTEEGYPTHTGMWLAIILAALIFGM
                     GHLPMVMALATLTPLLVMKVIILNAVGGIIFGWLYWKKGLESAMISHFSADIVLHVIL
                     PLIAVI"
BASE COUNT         1647 a         1130 c         1076 g         1659 t
ORIGIN      
        1 ccttctcacc gcccaggtgt tttctgcccc agaaatccag atcctcctgg aagagggctg
       61 gtccggcgta ggtttccagg aggtcgatgt catccgggtt aagggggaag cccccggcca
      121 ggccagtgag gtcgtagagc ttttcccggc cattttccct caccaagtcg gcgatgtagt
      181 gcagggctgt ttcccgtctt ttaatttcat ccagtgagga gttgaccagc ataaactgtt
      241 cccaccaaaa atcagtctat tttctcccgt ggtttttacg gctgtcttcc ggggttaaca
      301 caatggcact gtctgccgag ttctgtaagg cagcgctgaa cccgggctcg gcacctatga
      361 ctatggtttc cttaccattt tccttggcct tatttattat aggtagaaaa tcagcgtcac
      421 gggtcatgag ggcgatgacg tcgatgttag gattgtaaat aagttccata gcctccacgg
      481 ccatgtagac atcggtgtca ccagcaacca caatgggggt gaatccctgg ttaactatgg
      541 cttctatgag tttatcagag gcgtactggt taagaagaac tttacccacc cgcatgttcc
      601 catactcagc gattatatcc cgcaccagat ccagattaag actgaattcc ttgcggagca
      661 tgttaggacc atccaccagg aggcctatgt tcttaccccc ggattcgctc ctttttaatg
      721 gtatgtaatc tttaagagag gttaacctct caaaactacg catttttatc actccaatcg
      781 ttgcaattgt tataaaaatc aggggttttg aaaaaaaatt gggataactc ccacccctat
      841 ctatcttaaa ataactcaaa cccaaaaggg tggggtggga tctgcttatt tatggctaat
      901 taaatttttc aacttcctga caattttttt aatctctaca tatcttatga ttttcctgga
      961 ttataataat tttcataaaa gaacaaataa atttcaaagc ccccatccct attttgccct
     1021 gaaaagatca tcaaatctta tattaaccat aaatggatta gaagctttta aatcgtaaca
     1081 taaccaaata acctattttc attccatttc cgcatcaaat tcatctttaa aaagttcctt
     1141 aaccacttcc agctcgttac cagacagaga acatatttta acttcatctt tatcctgaaa
     1201 gtagtactcc gcgttgacta ttccctcagc atcacccatg tacaggtata atccattcac
     1261 atactcctct ggattagtag atggtaaaaa tacctctatt cggatcagga ggggtaagtt
     1321 atcaaggaat tcctgttttt gggcctcgtt ttttagggca gctttgaact ctttcatacg
     1381 cttttgaagt tctttgttaa ccttggaatc agcgtcactc atatattcac catacataat
     1441 ctgaacttag tttaatggtc ccctgttaaa gattggtctc taacaagatg gaatccatgt
     1501 aggagttata atggatttac ttttaatatt tatcctttga ggaaaagtag tggagcttac
     1561 cccaagctaa gccaggaagg gtcagaccat ttaccatccc ctattcctat caataccgat
     1621 aaagaggacc tttaagagca caccacatcc accagaatca gtgatgggtg aaaagcaaag
     1681 tgtcgcctga tctttgcaac tacccacctc aatccctgca ggttagacca ccaccccggt
     1741 gacttcatct aatccttcac aaagaaaatg gggactcggt ggagacttca tttagaacct
     1801 tttctatctt ttcattattg ggaatggcca gccacacgga tccttcagca ccagccaccc
     1861 caccagccgc caccaacttt gccttggccc ctgtgaggaa ttttatagcc tctatttcgg
     1921 tgaaaacctc tcctggcact ggtaacaggc ggggtccctt gaggcctggc tggttcaatt
     1981 ccactgccag ttcgttcagg tcactgctca ctcttttttc caaacccacc ggtaagataa
     2041 gcctcaccct tctacccacc acccctggta gggcggcgcc tatggtacct gcctgggggt
     2101 ggccgatgaa gatggcggcc tgtttaagat ccaggttaag ggcgttagcc cccttcaaga
     2161 tgatgtctcc ctcttttaag gagtcaacca catcgaatat cctcattccc ttttcccaga
     2221 ccccatctgt gattatcaca tctcctggga agccagcgtc atcagcgtag cgtccatccg
     2281 gattcacagg agcccctggt ggcagcacaa ttcccctgaa gaatcttttt ctggagaaat
     2341 cattttcctg acccatactt ttcaatattt cttcggccac gtaaccattg gtggtgccag
     2401 cgatgattgc cagggtgccg gatcgaagtg tttttttaat ttcaggatgt tccatcaggg
     2461 cttttgcaat gagtctttta cctgctgcag gggtgattag aaactgtttc atgatactaa
     2521 atgattctat gagtttctat aagttacgga atccaccttc cccgttgtca tttaaaggcg
     2581 gagccaacct ttaaaaaaaa ttcccttacc aatggataca aatttatcat cagttactct
     2641 tccatgggtg tttttcacct cctgataaga attcaatgcc attttaagag gtgctgcaaa
     2701 aattgataat caaaagtagg ctaaaaagta ttaatgacat aaaaataata gggcatcagt
     2761 atacgttgtg aggatgatga tcgatgccct actattggta acctgaacca tggacccatg
     2821 gcttataaaa aaaattacat attaagtaca cgggtttatg gtaatacttt attatttctt
     2881 atcatataat atgattaatc attacatata tagttagtga tatgttactg atcaattctc
     2941 caaaaaaagc agaatatttc tgtaaaaaga ttagtaattg ttcttttaac cttgtagatg
     3001 cactaaaatc caggtatgca gtgttggtgt agatagttca caccgtgtgc aatgtaaaaa
     3061 tatattaatc atcaaaatca tactaaatat tgtcctggat actgagtatt gttaacaaca
     3121 ggaaaatggg ggggaagctt tgttctcatt ggtaattccc acaagaggtt gggggttaat
     3181 ctgccagttg attctggacc agttattgga tattaacata aggcattttt ccatttattc
     3241 aggctatgaa ctattttaca tcctattgtt cccatctttc caggtaaata ttgcgaattc
     3301 tgcttctcct ggctcaataa ttattcgaaa ttgtgatctg caaaggcgag gtgaaaaaga
     3361 tggataaaga gctatacgtc aaagaggcta tacagaatag ggttgacttc gtggagccct
     3421 taaaaaaaac tggatacaat atgggagaac tccatgcctt aaaatgggtt ctgaaacttt
     3481 taaatgatga gatactggtg tatgatgagg aaacttactg gaagttgatg aaatctgaaa
     3541 aagattgccc tacctgtaat gccatactac tacgggtaac gggaaaatat aatgggacct
     3601 atgaatccta tgctccacct aatgagtatg gaagatccct taaggaacat ttaacaagtg
     3661 taaaagaagc tgatggatgc atgggagata catgtatcga aaaagatgga gagaaaatag
     3721 taatgatcta ctctgagaat aaatgactgc cctttaaatg atgagcggat tcataaaatt
     3781 agattcctaa cccaacgatt aattattgat tcttgtttag ttataaaact agttaatacc
     3841 taattttcat tagtttttaa ctgaaatagt tattaagtca acacctccag aaatggggaa
     3901 gctttaaata catcttcgga cattttacca tatagtgtca ttatatgaca ttaaggtaaa
     3961 ttatgttatt taatgacaat taatttagaa atgtcgttat gaagggattg gaatgcccat
     4021 atcagacagg aaagaaagag aaaaagaaat gcggcgaaaa gatatcatcg acacagccga
     4081 gaaattaatc tttgaaaatg gttatgaaaa cattaccatg gctgacatag ctaaaggtag
     4141 tgaattagcc agaagtacat tgtatctcta ttttaaaaat aaaaaagaga tatatctatc
     4201 catatctatt agaggtacgc agattctaaa caaaatgttc aaagaatatt ataatagagg
     4261 cgaaacagga ctagaaaagg ttaaaatgct catgattgca ttttataaat tctataaaga
     4321 atatcctggc tattatgatg ttaactggct ttcatacccc gttgcattta atcatgatct
     4381 gccgaaaatg aaagaagtaa agagaatgag gatggaaggc ttttcattgt ttggaaaagc
     4441 acttcaagag gggatagaag ataggtccat tagatccgat atagaccctg tgaaaacaaa
     4501 tcttgttcta gctttgtcta tacaaaatgt gttaaatctt ccccctacaa tggagttgca
     4561 catgaaaaat aacaatctga cccataagga gctaattgat tataccatgg acctgatgct
     4621 gcattcatta gaataatttt atgggggaga ttaatatgca aagtaagagc aaaaccaaga
     4681 aaaaaagttt catatcaaaa gatactgtta actggaaatt gtatttaatt ttactgggtg
     4741 catgtatctt tggaaccctg gcggtactgc cgtatactct cacccttcaa ggaggattac
     4801 tggaaaattc cacggtaccc ttgtacttca tactggctgc tcagattatc cagagtatag
     4861 tcctgttctc agttaccata tttattggcc tgttactctc taaaaaggtt ggactggaac
     4921 tacccatact ggaaggatgg ctggggggca gggaagttaa aaactattta aaatccgtac
     4981 tgggaatttc aattgggctc ggtatcctgg ccagtatcat gataatagga attgactatt
     5041 tgttttcctc caccggactg accagtagta ttgttatggc ccagataact cccccccgcc
     5101 tggcaaggac tcctttcatc attttatggt ggaataaatg aagagatcct cctcagatta
     5161 ttcctgatga ccctaattac ctggataatc tttaagatca gaaaaacaga ggaaggatat
     5221 cctacccaca ccggtatgtg gttagctatt atcttagccg cacttatttt tggtatgggc
     5281 cacctgccaa tggtcatggc cctagctacc cttacacctc tactggttat gaaagtgata
     5341 atcttaaatg cagtgggtgg aattatattc ggatggctat actggaaaaa aggtttagaa
     5401 tccgccatga tatcacattt ttctgcagat atagtgctgc atgtgatttt accactcata
     5461 gcagtgattt gaagcctaag ttatccattc atttttcttt cttttttata tt
//
LOCUS       DAOQVW010000034         5497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_16324_length_5497_cov_3.300074, whole genome shotgun sequence.
ACCESSION   DAOQVW010000034 DAOQVW010000000
VERSION     DAOQVW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16324_length_5497_cov_3.300074"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKK85_01360"
     CDS             complement(<1..313)
                     /locus_tag="PKK85_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24706.1"
                     /translation="MDSIIEDIRVRKILDSRGNPTLEVDVITWNGFGRAAAPSGASTG
                     SREVVAFPEGGVDKILGELEDVISTELIGMDAEDLREIDLVLKEIDGTEVETPTQPTA
                     AN"
     gene            complement(375..557)
                     /locus_tag="PKK85_01365"
     CDS             complement(375..557)
                     /locus_tag="PKK85_01365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HNS24707.1"
                     /translation="MTKSKLTRFERARIIGARALQLSMGAKPMVEVTGSLDPIDIAIR
                     ELKENVIPLEVRHGPE"
     gene            complement(619..744)
                     /locus_tag="PKK85_01370"
     tRNA            complement(join(619..653,672..676,710..744))
                     /locus_tag="PKK85_01370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(673..675),aa:Pro,seq:ggg)
     gene            complement(744..911)
                     /locus_tag="PKK85_01375"
     CDS             complement(744..911)
                     /locus_tag="PKK85_01375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HNS24708.1"
                     /translation="MIPIRCLSCGKVVSAYFDEYQKRVQDGEDSKKVLDDLGLKRYCC
                     RRMLISHVEVW"
     gene            complement(954..1355)
                     /locus_tag="PKK85_01380"
     CDS             complement(954..1355)
                     /locus_tag="PKK85_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNS24709.1"
                     /translation="MKKVIHTSGKRKTAIARGKVREGKGRVRINKLPVEYYHPELARL
                     KIKEPLTLAGDLVDQVDIDVHVVGGGVMGQAEAARMVIAKGLVQWTGDMDLKEKFNQY
                     DRTMLVGDPRRTESKKYGGRGARARQQKSYR"
     gene            complement(1373..1795)
                     /locus_tag="PKK85_01385"
     CDS             complement(1373..1795)
                     /locus_tag="PKK85_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNS24710.1"
                     /translation="MIIDGEGLILGRLASIVSKKLLDGEEVTILNSEKIIISGTKDWA
                     YAKYKQRIDRASISNPRRMGPKYPRRPDDIFRRTVRGMLPYKQAKGREAYKLLKVYLG
                     IPQEFQGQELSPVEEAVPCNVAKSVELGKLSQLLGAKF"
     gene            complement(1813..2166)
                     /locus_tag="PKK85_01390"
     CDS             complement(1813..2166)
                     /locus_tag="PKK85_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HNS24711.1"
                     /translation="MTKTNPQIMRIIEILKERSYQEGAALWKEVARRLEKPTRQGAEV
                     NLSRINRHSSPDETVLVPGKVLGSGSLDHKVRVVALDFSQSAAEKIIGAGGECLDIYQ
                     LLEENPQGSGVRMIE"
     gene            complement(2184..2969)
                     /locus_tag="PKK85_01395"
     CDS             complement(2184..2969)
                     /locus_tag="PKK85_01395"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HNS24712.1"
                     /translation="MEIEIISQDNQELVFRIEGVEVSLVNALRRIVMVEVPTLAIEEV
                     LFLKNEARIFDEALAHRLGMVPLTTDLEAITPRGECDCGDHCSRCSVSLTLKGKGPKT
                     LYSGDLESSDPQVKPVLDTIPLVKLQKGEEVELEAIAQLGTGIEHAKWQPTTTCAYKN
                     YPQITIDQDKCEACGLCAQQCPRGVYFFDEKENKIQVVDLENCSMCRTCSKDCPNQAI
                     TVEGLGDKFIFRIESDGSLPPWEILTTACDILFQKAEKILDYL"
     gene            complement(2991..3386)
                     /locus_tag="PKK85_01400"
     CDS             complement(2991..3386)
                     /locus_tag="PKK85_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNS24713.1"
                     /translation="MAERKEKWGVANVYSSFNNTIITITDLTGAETITQWSGGKVVRA
                     DRQESSPFAAMEAASRAADDAREKGFTGLHIKVRAPGGNGPRTPGPGAQATIRALARA
                     GMRIGKIEDVTPVPHDGTGRPGGKRGRRV"
     gene            complement(3402..3932)
                     /locus_tag="PKK85_01405"
     CDS             complement(3402..3932)
                     /locus_tag="PKK85_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNS24714.1"
                     /translation="MGHPRKSRKNYDTPSHPWNADRIKDENRLVNRYGLKNKKEIWKA
                     ETKIRRYRREARVLLGTESEQTQREREQLLGSLTRNGIMAESSKLEDVLDLTVEDVLR
                     RRLQTMVHKQGLAHTVKQARLMVVHGHIALQGRKINSPGYLVKRGEEEHIGFYPGSPM
                     EQQIKKPTQENKGGKE"
     gene            complement(3948..4403)
                     /locus_tag="PKK85_01410"
     CDS             complement(3948..4403)
                     /locus_tag="PKK85_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNS24715.1"
                     /translation="MEEDFKHMVRIARKDVDGNKTIINAITSIKGVGKALSGALCSIM
                     EYDPDQKIGYLSDEDVLKLEEVILNPQKLEKLPRWMLNRRNDYATGEDFHLIESDLVL
                     CLRDDLNRMRKTRSYKGRRHEMGLPVRGQRTKATFRKGSSVGVRRRRRG"
     gene            complement(4575..4660)
                     /locus_tag="PKK85_01415"
     tRNA            complement(4575..4660)
                     /locus_tag="PKK85_01415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4624..4626),aa:Ser,seq:gga)
     gene            complement(4726..>5497)
                     /locus_tag="PKK85_01420"
     CDS             complement(4726..>5497)
                     /locus_tag="PKK85_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HNS24716.1"
                     /translation="GGTLDPRVTGVLPVGVDDATRAIQLLLSAPKEYVCLMRLHHPVA
                     QARVCQVLEEFTGKIFQTPPLRSAVKRELRVRSIYYVHILEMDEQDVLFRIGCEAGTY
                     IRKYCHDVGEALGVGAHMAELRRTRVGSFQEDTLHTLQDLTDAYVAWQEGDETLLREC
                     LLPMEAAASHLPLMVVRDSAVDALCHGADLAAGGILALSPDLKSGDMVRVETLKGELV
                     AAGLTLQPSREIQQLSQGIMVDIKKVFMEPGTYPRMWK"
BASE COUNT          992 a         1545 c         1400 g         1560 t
ORIGIN      
        1 tgttggcggc cgtgggttgg gtgggcgtct cgacctcggt gccgtcgatt tccttcaaaa
       61 ccaggtcgat ctcccggagg tcctcggcat ccatgccaat gagctccgtg gatatgacgt
      121 cctctaattc gcctagtatc ttatcaaccc ctccttcggg gaaggccacc acttcacggg
      181 atccggtgct ggcgccgctg ggtgcggctg cccttccaaa tccgttccag gtgatgacat
      241 ccacttccag ggtggggttt cctctgctgt ctaaaatttt tctaacccgg atgtcttcaa
      301 taatactgtc cacaaaaaca cctcatttca taaaaataac tgtttattag ggttttcagg
      361 cttattaagt gggcttactc agggccatgc cggacttcca ggggaatcac gttctcttta
      421 agctcccgga tggcgatgtc aatggggtcc agtgaaccgg ttacttccac catgggttta
      481 gcccccatgg agagttgtaa agcccgggca ccgataattc tagccctctc aaagcgggtg
      541 agtttactct tagtcatttt ctctccattc tcctaaaatt aggtgaattt tttccttagt
      601 tatctaaatg aatttaaatg gggccgccga gatttgaact cgggtctcca gcgttccggt
      661 gttttgacca tccccgttcc agttgaagaa actagaaact tccttcaatg gctgggagga
      721 tggaccaagc taccctacgg cccctaccat acttctacat gagatatgag catccgacgg
      781 caacagtaac gtttaagtcc cagatcatcc aggacttttt tggaatcctc gccatcctgg
      841 acccgtttct ggtactcgtc gaagtaggcg gagaccacct taccacagct caagcatctt
      901 atgggaatca ttatttcctc tttttataat ataaaataat ggggttatta attctatcgg
      961 taactcttct gttgacgcgc ccgggcaccc cttccaccgt actttttgga ttctgtccgg
     1021 cggggatcac ccaccagcat ggtccggtcg tactggttga acttctcctt gaggtccatg
     1081 tccccggtcc actgcaccag tcccttggca attaccatgc gggctgcttc ggcctgtccc
     1141 attactccgc cacccaccac gtgtacgtcg atgtccacct ggtccaccag gtcaccggcc
     1201 agggtgaggg gttccttgat tttgagtcgg gccagctcgg ggtggtagta ctctacgggt
     1261 aacttgttga ttcgcacccg gccctttcct tcccggactt tgccccgggc aatggctgtt
     1321 tttctttttc cactggtatg gattaccttc ttcatggtta acacctttaa atctaaaatt
     1381 tggctcctag tagttgggat agttttccca gctccactga tttggccacg ttgcagggta
     1441 ctgcttcctc cactggggac agttcctgtc cctggaactc ctggggaatg cccaggtaga
     1501 ccttcaggag cttgtaggcc tcacggccct tggcctgctt gtagggcagc atgcctcgca
     1561 ctgtccggcg gaatatgtcg tcgggtcgtc gggggtattt aggccccatc ctccgggggt
     1621 tggagatgct ggcccggtct attcgttgtt tgtacttggc gtaggcccag tccttggtac
     1681 cggagatgat gatcttctcc gagttgagga tggttacttc ctctccatcc aggagtttct
     1741 tactgacgat actggccagt cggcccagta tcagtccttc tccatcaata atcatttata
     1801 atacaccttc ttttattcaa tcatccttac cccacttccc tgggggtttt cttccaagag
     1861 ttgatagata tccaggcatt cgcctccggc tccgattatc ttttcagctg cggattggga
     1921 gaagtccagg gccaccaccc ggaccttatg gtccaggctg ccactcccca gcaccttacc
     1981 cggcaccagc accgtctcat ctggggagct gtgccggttt atccggctga ggtttacttc
     2041 cgcaccctgg cgggtgggct tctccaggcg ccgggccact tccttccaca gggcggcccc
     2101 ttcctggtac gatctttcct tgaggatctc tatgattcgc atgatctgag ggttagtttt
     2161 agtcatttta aacttcctcc ttgttataaa tagtctagga tcttttctgc cttctggaac
     2221 agtatatcac aggcagtggt gaggatttcc caggggggta atgatccatc ggattctatg
     2281 cggaatatga atttatcccc cagtccctcc acggttatgg cctggttagg gcagtccttg
     2341 gagcaggtgc ggcacatgga acagttttcc aggtccacca cttggatctt gttctccttt
     2401 tcatcaaaga agtacacacc ccgggggcac tgttgggcgc agagtccaca ggcctcgcac
     2461 ttatcctggt caatggtgat ctgggggtag ttcttgtagg cacaggtggt ggtgggttgc
     2521 catttggcgt gttctattcc tgttcctagc tgggctatgg cctccagttc cacttcctca
     2581 cctttctgga gtttcaccag gggtatggtg tccaggactg gtttgacctg gggatccgat
     2641 gattccaggt ccccggagta caaggtcttg ggtcccttac ccttcagggt caaggaaaca
     2701 ctgcaacggg agcagtgatc tccacagtcg cattctcccc ggggggttat tgcttccagg
     2761 tcggtggtga ggggcaccat ccccagccgg tgggccaggg cttcgtcgaa tatgcgggct
     2821 tcgtttttca gaaacagcac ctcctcgatg gccagggtgg gtacctccac catgactatc
     2881 ctccggaggg cgttgaccag ggacacttcc accccttcaa tcctaaacac cagttcctgg
     2941 ttgtcctggc ttatgatttc tatctccatt ctagacacat cattacttag ttagaccctt
     3001 ctaccccgct tgccaccggg tcgtcccgtt ccatcgtggg gtacgggggt gacgtcctca
     3061 atttttccta tcctcattcc agcccgtgcc agggccctga tggttgcctg ggctcctggt
     3121 ccaggggtgc ggggtccgtt gcctcctggt gctcggactt ttatgtgtaa acctgtgaat
     3181 cctttttccc tagcgtcatc agcagcacga ctggctgctt ccatggcagc gaatggtgat
     3241 gattcctgtc gatcggcacg caccacctta ccaccagacc actgggttat ggtttctgct
     3301 ccagtgaggt cggtgatggt gatgatggtg ttgttgaagg acgagtacac gttggctact
     3361 ccccatttct cttttctttc agccatattt atcacctgtt attattcctt tccaccctta
     3421 ttctcctggg tgggcttttt tatctgctgt tccatgggtg agcccgggta gaacccgatg
     3481 tgctcttctt ctccacgctt caccaggtaa cccggggagt ttattttcct cccctggagg
     3541 gctatgtgtc cgtggaccac catgagacgg gcctgtttca cggtgtgggc cagtccctgt
     3601 ttgtggacca tggtctgtaa gcggcggcgc agcacgtcct ccacagtcaa gtccagaacg
     3661 tcttccagtt ttgatgactc ggccatgatt ccgttccgcg tcagactgcc caggagttgt
     3721 tcccgttccc tctgggtctg ttcgctttca gtacccagga gtactcgggc ttccctccgg
     3781 tacctgcgga tcttggtctc cgccttccag atctctttct tgtttttaag tccgtatctg
     3841 ttcaccaacc ggttttcatc cttgatacgg tcggcgttcc aggggtggga tggtgtgtcg
     3901 tagtttttcc ttgattttct gggatgtccc atgtgatcat ctccggtcta tcctcttctt
     3961 ctccttctaa ctcccactga ggagccctta cggaaggtgg ctttggtgcg ctggccacgt
     4021 actggcagtc ccatttcgtg ccttcttccc ttgtagcttc tggtcttcct catacggttc
     4081 aagtcatccc ggaggcacaa gaccaggtcg gattcgatga ggtggaagtc ctctccagtg
     4141 gcgtagtcgt ttctgcggtt gagcatccag cggggtagtt tctccagttt ctgtgggttg
     4201 aggatgactt cctccagctt cagcacatct tcgtcagata ggtacccgat tttctggtcg
     4261 gggtcgtact ccattataga gcataaagca ccggataatg cttttccaac tccttttata
     4321 ctggttattg cgtttattat ggttttgttc ccgtctacgt ctttacgggc gatccggacc
     4381 atgtgtttaa aatcttcttc catgtctttt ttcctcccag ttgggggttc agggctcggg
     4441 ggcggatcat cagctcctcc accatcaggg gtgggggtct gaaacttcta agtacctcca
     4501 gccctattca ttactcctaa tcgtacaaat ttcctgccta ttttagtatt taagggtttt
     4561 tgtagtttta gaaacgccgg gactgggatt tgaacccagg cggagccaat gctccacaag
     4621 atttccagtc ttgcgcctta ccaggctaga ctatcccggc atgttgaata ttataccctt
     4681 atcttcttta cattctggta agtgtatggt agaaatagca ggtcttcact tccacatgcg
     4741 tggatatgtt ccaggttcca taaatacctt ttttatatct accatgatcc cctgggagag
     4801 ttgctgtatc tcccgggatg gttgcagtgt caggccagca gccaccagtt cccccttgag
     4861 ggtttccacc cgcaccatgt cgcctgattt cagatccggg gatagggcta gtataccccc
     4921 tgcagccagg tctgcgccgt ggcacagggc atccactgcc gagtcccgga ccaccatcag
     4981 ggggaggtga ctggcagcag cttccatggg caggaggcac tcccggagta gtgtttcatc
     5041 accctcctgc caggccacgt aggcatcggt caagtcctgc agggtgtgca gggtgtcctc
     5101 ctggaaggat cctaccctgg tgcggcgcag ctccgccatg tgggccccca cacccagggc
     5161 ctcccctacg tcgtggcagt acttacgtat gtaggtaccg gcttcgcagc caatacggaa
     5221 aagtacgtcc tgttcatcca tctccaggat gtggacgtag tatatgcttc gcacccgcag
     5281 ttccctcttc accgccgagc ggaggggtgg ggtctggaat atctttcctg tgaattcctc
     5341 caggacctgg cacacccggg cctgggccac ggggtggtgg agtcgcatta gacacacgta
     5401 ctccttgggt gctgatagaa gtaactggat ggcccgggtg gcatcatcca ctcccaccgg
     5461 caaaaccccg gtgacccggg ggtctagggt gccgccg
//
LOCUS       DAOQVW010000035         5427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_16591_length_5427_cov_4.019732, whole genome shotgun sequence.
ACCESSION   DAOQVW010000035 DAOQVW010000000
VERSION     DAOQVW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16591_length_5427_cov_4.019732"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..362
                     /locus_tag="PKK85_01425"
     CDS             9..362
                     /locus_tag="PKK85_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF22 domain-containing protein"
                     /protein_id="HNS24717.1"
                     /translation="MHDHEKPPIDFQIGTISGKVRAIIADEDREFKAGQIKSVKIKKI
                     SINAKHISLISAYAANKYGHAMAVGEETYLPISMDRTADHAQFAAALDCKIEKDDLLG
                     ILILLPVKLTGRWKE"
     gene            431..595
                     /locus_tag="PKK85_01430"
     CDS             431..595
                     /locus_tag="PKK85_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24718.1"
                     /translation="MDISFLWKSDWEEIKKDPDKMAKVFMLISGALILTTILIVIGTI
                     IFILRVLGLV"
     gene            complement(873..1286)
                     /locus_tag="PKK85_01435"
     CDS             complement(873..1286)
                     /locus_tag="PKK85_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24719.1"
                     /translation="MMEANDYYKRIMELADLDVEAKTIAESRMILEELEEREVQLKDL
                     HLELKRDIRYVEAIYIKRKRELNQAFNQVDDLPKKRFAKKKESRVAVLKKLEKEKQEV
                     IGPYYQVCDMAEQLLCEMDEAKKPLQDFVIDKMGL"
     gene            complement(1341..1676)
                     /locus_tag="PKK85_01440"
     CDS             complement(1341..1676)
                     /locus_tag="PKK85_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24720.1"
                     /translation="MDIDIEQCRENDKIKEIISNSGLSIKHIKLLLRLSDAIYINAIN
                     YNVVVEDNRFIILLISSKPENQTGVFNTISLTNVLFRLKEMERDHDDLSTRCEVEDKM
                     LKVTIDLKT"
     gene            1742..2152
                     /locus_tag="PKK85_01445"
     CDS             1742..2152
                     /locus_tag="PKK85_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF61 family protein"
                     /protein_id="HNS24721.1"
                     /translation="MRLDQDRSDRLLKKQILNLNRHIPRQRKAVSELIAEDKPHVMGV
                     DGSRHRFKRKELEFVVSMLSDSELDILKLPIYIEMDTMASGARVSGKLETRILCKILE
                     REECPADEIFIYRPEMRIIRQKLPTVTQYMFLVR"
     gene            complement(2171..3304)
                     /locus_tag="PKK85_01450"
     CDS             complement(2171..3304)
                     /locus_tag="PKK85_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNS24722.1"
                     /translation="METILVVGVNTRAVACSLKKLGYKVYSADYFGVTDLENCTYLSR
                     SIVKQRAGESCGYFKKDFNPGDLKKNALSLMDWVDKVICLSGCSPSDFPPAKIYGNPS
                     PWDDKGLLYHALKDDFLLPLTYMPGSIAEAREIIQQYPDKKFIIKPKSGAGGYGIRVW
                     DDPENWPVDWLLQEHIIGDNISASVLSTAEESRTIITSQQIIGAGELGQKEPFGYTGN
                     ITPYCGQANIVELAEIVINRLGLVGSNGVDFIAKKGEVYVVEVNPRIQGTFECVEKSL
                     GINMAKAHIEACKGRIVPIPTPQKFAVKMVVHAQERSKAGRINFRDVHDLPLEGVIIE
                     AGEPAATILSSDSVMENAIHKANLGVTSVYENLSPYNKPIISP"
     gene            complement(3327..3398)
                     /locus_tag="PKK85_01455"
     tRNA            complement(3327..3398)
                     /locus_tag="PKK85_01455"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3363..3365),aa:Gln,seq:ttg)
     gene            complement(3477..4142)
                     /gene="cas4"
                     /locus_tag="PKK85_01460"
     CDS             complement(3477..4142)
                     /gene="cas4"
                     /locus_tag="PKK85_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNS24723.1"
                     /translation="MKDSHPTYHPDIAKVMIIEGQNNFPISWLNVQGYCEYSIYLENV
                     KGIRTAPTRAMKSGSKIHHVLEEEFKRGAIPTTMEEMMETSKTTEIFSRELYVESVHY
                     GIRGLIDEIWMTPDEFIIIDDKPGDIAYPSLINQVFGYCLAFKDRFWDGRRIVASLRQ
                     SDTGDIFWQQYFDEEAENQIKNIVDQIHQALLFNREFIPTKNPNKCRKCRFNTKCDRR
                     VES"
     gene            complement(4314..4868)
                     /locus_tag="PKK85_01465"
     CDS             complement(4314..4868)
                     /locus_tag="PKK85_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24724.1"
                     /translation="MVKLIKSTVKCYRKKTKKTVGGKRKTYEYNQYLVPLKRSDQLEC
                     SQDVFVVPENELEKMLDEEGQLVGDKKELQRHVDAYQKELDELEWKHGELSRSYKDLV
                     AKNTKNNQEIRRLTAELENLRLKNQELESDLEVERENHQALEEKYQLEVKKTQLLEEE
                     LSKHKGDFWTAIKSKLASREEKNP"
BASE COUNT         1490 a         1167 c         1038 g         1732 t
ORIGIN      
        1 aaagggagat gcacgatcac gagaaaccac ctattgattt tcagataggc accatatccg
       61 ggaaagttcg tgcaattatt gccgatgaag accgggaatt caaggccggt cagataaagt
      121 cggtgaagat caagaagatc tccatcaacg ccaaacatat atcccttatc agcgcctatg
      181 ccgccaataa atatggacat gccatggctg ttggtgaaga aacatatcta cccatatcca
      241 tggatcgaac cgctgatcat gcccagttcg cagcagctct tgattgcaaa attgaaaaag
      301 acgacctatt gggtatttta atacttcttc cagtaaaact aaccggccgc tggaaggaat
      361 aaaatttact gcccaatatt ttttttattt tatggttttg gtaaagttaa ttcttttgtg
      421 gtgaaatcaa ttggacataa gttttttatg gaaatctgac tgggaagaaa taaaaaaaga
      481 cccggataag atggccaaag tcttcatgct gatctcaggg gccctgatcc tgactaccat
      541 tttaatagta attggaacca taatatttat tttaagggtg ctgggtttgg tgtgaggata
      601 gcttgtccca aataggccat atatttaatg gtaaatttat tttttaaaaa aaggctttct
      661 gtgttggaaa aaaatagaat aagtaaaact tgatcttaag ctactcagca gggtttcggt
      721 ttaaaatctg tatcaggata tttaccagta cgaaaccgcc taatataacc cctaaaatgt
      781 accatgaata atccattgat atactcacct cttaccgata atagatagtt aacccttatt
      841 atagaaatgc tttttcaatt tactttgatt tttcacaacc ccattttgtc tattacaaag
      901 tcctggagtg gtttttttgc ttcatccatt tcacagagaa gttgttcagc catgtcacac
      961 acctgataat agggacctat aacttcctgc ttttcctttt ctaatttctt caaaaccgcc
     1021 acccgggact cttttttctt tgcaaaacgt ttctttggaa ggtcatccac ctggttaaat
     1081 gcctggttaa gttccctttt tcgtttaata taaatggctt ccacataccg gatgtctctt
     1141 tttagttcca gatgcagatc ttttagttgg acttcccttt cttcaagttc ttccagtatc
     1201 atacgagatt cggcgatggt cttggcctcc acatccaagt ccgccagttc cattattctt
     1261 ttataataat cgttagcttc catcataatc tcccctttgg gattggctca tttgctcaca
     1321 ccaagatcat gaaaagcttg tcaagtttta agatctatgg ttaccttcag catcttatct
     1381 tcaacttcac agcgtgtgct tagatcatca tggtcccgtt ccatttcctt cagacggaat
     1441 aatacgttgg tgagggagat ggtgttgaac acacctgtct ggttctcagg tttagatgat
     1501 ataagaagta ttataaaacg attatcttct acaacgacgt tgtaattgat ggcgtttatg
     1561 taaatagcat cagatagcct taaaagtagc ttaatatgtt tgatagatag tccactgttt
     1621 gaaatgatct cttttatctt gtcgttttca cggcactgtt caatgtcgat gtccatttaa
     1681 ccacccatct taccattcgc tagaagatat tatactccac cattttaaat aaattgtttg
     1741 tatgagactg gaccaggatc gatcggatcg gctgttgaaa aaacagatcc ttaatcttaa
     1801 tcggcatatt ccccggcaga gaaaagcagt atctgaactt attgcggaag ataaacccca
     1861 cgtgatgggc gtggatggtt cacgtcatcg ttttaagagg aaagagctgg aatttgttgt
     1921 ttcaatgtta agtgattctg aactggacat tttgaagcta ccaatctaca tcgaaatgga
     1981 caccatggct tctggggccc gtgtgtccgg caaactggaa accaggattt tgtgtaagat
     2041 cttggaaaga gaagaatgtc cagcggatga aattttcatc taccgacccg aaatgaggat
     2101 aatacgccaa aaattaccta ctgtaactca gtacatgttc ctagtgaggt agattgtccc
     2161 gcaccacggt tcaaggacta ataattggtt tattataggg ggataaattt tcataaacac
     2221 tggtcacacc caaattggcc ttatgtatgg cattttccat gacgctatca ctgctaagga
     2281 tggtggctgc tggttctccg gcttctatta ttacaccctc cagggggaga tcatggacgt
     2341 ccctgaaatt gatccttcca gcttttgatc tttcctgggc atggactacc atcttaaccg
     2401 cgaatttctg gggtgtggga attggtacta ttcttccctt acaagcctct atatgggctt
     2461 ttgccatgtt gattcccaat gatttttcca cacactcaaa tgtgccctga attcgggggt
     2521 tcacctccac tacgtaaact tccccctttt ttgctatgaa atccactccg ttcgatccca
     2581 ccaagcccag gcggtttatt actatttcag caagttccac tatatttgct tggccacagt
     2641 agggggtgat gttgcccgtg tatccgaagg gttctttctg tcctagttca cccgccccta
     2701 taatttgctg actggtgatg atggttctgg actcttctgc agtgctgagg actgatgcac
     2761 ttatgttatc accgattatg tgttcctgca gtagccagtc caccggccaa ttttcaggat
     2821 catcccacac cctaattcca tatcccccgg caccactttt tggtttgatt atgaattttt
     2881 tatccgggta ctgttgtatg atctccctag cttccgcaat gctccccggc atataagtta
     2941 agggcagcag aaagtcatcc tttaaagcat ggtacaggag gcccttatca tcccagggcg
     3001 aaggattccc atagatcttt gcagggggaa aatcggaagg ggaacaacca gacaggcaaa
     3061 tcaccttgtc cacccaatcc attaaggata gagcattttt ctttaaatcc cctgggttaa
     3121 aatctttttt aaaatagcca cacgattctc ctgccctttg tttcacaatt gaacgcgaaa
     3181 gataggtgca gttctccaga tcagtaactc cgaagtaatc tgcagaataa actttgtaac
     3241 ccagtttctt aagggaacaa gccacagccc gggtgttaac acctaccaca agtatggttt
     3301 ccatgaaaaa tcccctttaa aagaaatagt cccgagcgga gtcgaaccgc cgtcgccggg
     3361 tccaaagccc agcaggatta ccactacccc acgggactaa tttataaaaa aggctgtggt
     3421 aatacagcac tcccttttta gttgaacgtc atatttaaag gtatcggagc ttgcagttag
     3481 gattcaaccc gacggtcaca tttagtgttg aatcggcact tgcggcattt attgggattt
     3541 ttggtgggta tgaactccct attaaacagc agcgcctgat gaatctggtc aacgatattt
     3601 tttatctgat tttcagcttc ttcgtcaaag tattgctgcc aaaaaatatc tccagtgtcg
     3661 ctctgacgaa gggatgctac tattctacgc ccatcccaaa atctatcctt aaaggctagg
     3721 cagtaaccaa aaacctggtt tattagggaa ggatatgcta tatcccccgg tttatcatcg
     3781 ataattatga actcatccgg agtcatccat atctcatcaa taagtcctcg aattccgtag
     3841 tggaccgatt ccacgtataa ctctctggaa aatatttcgg tagttttgga tgtttccatc
     3901 atttcttcca tggttgtggg gatcgcgccc ctcttaaact cctcttccaa gacatggtgg
     3961 atcttacttc cacttttcat ggcccgggta ggtgccgttc tgattccctt aacattttcc
     4021 aggtagatgc tgtactcaca gtagccctga acgtttagcc aggatatggg aaagttattc
     4081 tgaccctcaa taatcattac cttagctata tcgggatgat aagttggatg tgaatccttc
     4141 atggtttttc acttgctcct taatcctgtt cattattaac attagtggta tgaaaaatat
     4201 tataaaggtt gggtgggtgc agatgaaaag taatgaacaa taatcatggc aaaaaaatac
     4261 tgtggatttt agtcagggcg gaataacaag ttttagtagg acaccagttt tctttaagga
     4321 tttttttctt ccctactggc taatttactt tttatagcgg tccaaaagtc acctttatgc
     4381 ttggatagct cttcttcaag gagttgggtt tttttcactt ctagttgata cttttcctcc
     4441 agtgcttgat ggttctccct ttctacttcc aaatcacttt ccagttcctg atttttaaga
     4501 cgaagatttt ccaattcagc agttagtcga cgtatttctt gattattttt ggtattttta
     4561 gccactaagt ccttgtaaga acgggataat tcaccatgtt tccattccag ttcatctaac
     4621 tccttctgat atgcatcaac gtggcgctgc agttcttttt tatccccaac cagctgtcct
     4681 tcttcatcaa gcatcttctc cagttcattt tcaggaacca cgaaaacatc ctggctacac
     4741 tccagttgat cagatctttt taggggcacc aggtactggt tgtactcgta ggttttcctc
     4801 ttaccaccca cggtcttttt agtctttttc cggtaacact tgactgtgga tttaatcagc
     4861 ttaaccataa gacttacatc cccctatcaa caaaattcta ttgtcaaagt atacttttat
     4921 agttgctgca cctaagacca tattctaaat gagtcattta atatcatttt ttacacttat
     4981 agaagcttta caaactgata aactattttt tagatacaca cagaaccttt caaacttcag
     5041 gaatagttgt ggttttatct ccgactatca ctatcatcta atgttagtat tgtagtgtaa
     5101 ggagttttga atagactgtg aaaaaggagt gtccattgat agaagatact aattgaggat
     5161 agattatatg aaagaaagga tgaaaaagac tgcatgaatg gtaccaccct ccccatgttc
     5221 tttcctgtaa ttggaagtgt taagtgcact caatacggtt acatcaccca attttatgaa
     5281 aatcttgaac tagcagtgac gatgaaaaaa gattgagctc taggaaattt gcttaaacta
     5341 ccattgagtt gtgcaagtag ttatcattcg tcacatgcca taggaagggt tttccttccc
     5401 ccaaaaatcg aaaggtattt atcatat
//
LOCUS       DAOQVW010000036         5247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_17379_length_5247_cov_2.979199, whole genome shotgun sequence.
ACCESSION   DAOQVW010000036 DAOQVW010000000
VERSION     DAOQVW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17379_length_5247_cov_2.979199"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..677
                     /locus_tag="PKK85_01470"
     CDS             297..677
                     /locus_tag="PKK85_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNS24725.1"
                     /translation="MSEKRDITQHLKNQGQLSRFFCHRMPERTFKINGTYFPVCSRCT
                     GFYIGAFSYFIYVYFYYVDYNLMTIISSFLLLIPSFIDGITQFFNLRESNNILRLFTG
                     LLGGVGLAILIKAFKLFIFLNSIF"
     gene            717..1331
                     /locus_tag="PKK85_01475"
     CDS             717..1331
                     /locus_tag="PKK85_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNS24726.1"
                     /translation="MTKKCPECGNENPDNSSFCKNCGADLKGVSATPKPAAKPSKGGP
                     TGWWSEQSSLVKALSVIGICCVGLIIVAGIGGLLLPDSNTNTSTTPTTAPTTPAQTEA
                     TWHSVANFTGTGNKDTDSFTTKGQKFKVKITANTDSMEYALISFFAYPEGETKMYVGN
                     GGIDSFSKKSQTDEFIVNASPGKYYLTIIAANLKNWKVEVLDYY"
     gene            complement(2020..2370)
                     /locus_tag="PKK85_01480"
     CDS             complement(2020..2370)
                     /locus_tag="PKK85_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24727.1"
                     /translation="MLFKILVLALGYLLVAIFGVFIRILLRPYSSQIEDSGIQGAGFL
                     IGIFERILIFTFVLADQYAAISIIFAAKSIARFNELNERNFAEYYLLGTFISITLAIV
                     TGVIFKLVFGTLLI"
     gene            complement(2462..3100)
                     /locus_tag="PKK85_01485"
     CDS             complement(2462..3100)
                     /locus_tag="PKK85_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HNS24728.1"
                     /translation="MLESDLEYIVITGDLRSSKKIDKRSITQDNLKEAMKYLNTNFNE
                     HIVSEFQIVGGDGFQGMISAFDIIMDIYFGLFRKIRHPFYLGVGQGSISTSLSEHIQE
                     IDGKAFHFSKDALNTAKKKNRWIVLNSNLPNNDLTECVFNYIFDIMWSWTPRRSEIIS
                     FYRLHGENPQAIELAAHNFETGIRNIYKILEVGKYSLIKYGELVLEKEFKKK"
     gene            3670..4863
                     /locus_tag="PKK85_01490"
     CDS             3670..4863
                     /locus_tag="PKK85_01490"
                     /inference="COORDINATES: protein motif:HMM:NF018378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HNS24729.1"
                     /translation="MNKWFPVGLILLVVLLVSIAFLYQSMTDEERTILSRAPVHSQVE
                     LALTFYDHNLVRRELYSKYRATNNYEYYRSYLDSGYTVQVFSNLSHQNKLSTEEVKIL
                     ATTLKANNAYYTHHTSPHGDGIIGAFSTQSPYPTTNTFTSGKFTSKLPFVYYQGQGWQ
                     LYPVTATFWASEYFKQGNYKDGIALLDELSPYMSVEEYGGLDYGVFRVYFQYTNSSIP
                     WASSYSQGQAAGLYAQAYNHSKDGKYLDQSHLLFNSFKIPLSEGGFITSTPYGPWFLE
                     YNFKPRHLILNGHIITMQGIYHYYQVTGSQDALDLFNQGATSVKNSLPQMDSGDWSYY
                     ALTGVDAKPAWKANKYYMGLHVELLKWLHSTTGDSTFQYYAQRWEGFYKNRQGEVSTP
                     IITMP"
BASE COUNT         1489 a         1109 c         1097 g         1552 t
ORIGIN      
        1 gttcgcatct aggaatatta tgactggatc agaacgtaga aattgggatt tgaaaaggat
       61 agataagtac ctggatcatt gcttaaccat ttcttccatg ttctatcttc tgtgaaaaga
      121 ttccatagtg tttttttctt taattcgtta atatattttc tttacattgg caatatgtac
      181 caattatttg taaatactaa acactaacaa attaggtaaa agtttatatc tatggaaact
      241 actagtatca ttgtcttcat ggaagattat tgttctcagt aatgaatgga gaaattatgt
      301 ctgaaaagag ggatataact caacacttaa aaaaccaagg acaactatca aggtttttct
      361 gtcaccgtat gcctgaaagg acgtttaaaa ttaatggaac ctacttccca gtctgttcac
      421 gatgtaccgg gttctacatc ggggcattta gctattttat atatgtttac ttttattatg
      481 ttgattacaa tttgatgacc ataatatctt cattcttact acttattcct tcatttattg
      541 atggaataac ccagtttttc aatttaagag agagtaataa cattttaagg ttattcaccg
      601 gacttttagg gggtgttgga ctcgcaattt taataaaggc atttaaattg tttatatttc
      661 ttaattcaat tttttaatcg tccaaaaaag aattattcaa agggaggtat agtacaatga
      721 ctaaaaagtg tcctgaatgt ggaaatgaaa atccagataa ttcctctttc tgcaagaatt
      781 gtggagctga tttaaagggt gtttctgcaa ctccaaaacc agcagccaaa ccatctaaag
      841 gtggaccaac tggttggtgg agtgaacagt ccagcctggt taaggcgtta agtgtaattg
      901 gtatttgttg tgtaggtctg atcatcgtcg ctggaattgg tggattgcta cttccagata
      961 gtaataccaa caccagcacc acccctacca ctgcccctac tactcctgct cagactgaag
     1021 ctacctggca cagtgtggct aactttactg gcaccggtaa taaagacacc gattcattta
     1081 ccaccaaagg acagaagttt aaggtgaaga ttactgccaa cactgatagc atggagtatg
     1141 cactaatttc attctttgcc tacccagaag gtgagacaaa gatgtatgta ggcaacggtg
     1201 gtattgactc tttcagtaag aaatcccaaa ctgatgagtt catagtaaac gctagccccg
     1261 gaaagtacta ccttactatc atagcggcca atctgaaaaa ctggaaagta gaagtccttg
     1321 actactacta aatccaattt cttattcttt ttttttatta acatccctaa gagttgagtt
     1381 ttcaggactc cattcaggca ttaattggcc agttcttttt acatgacgga actagacgat
     1441 ctaataatat ctttctgggt atcaaatagg ctgctgagaa cacaaagacg aatcatatga
     1501 tgaatactag tccatggctc atatctcgaa agaacagtac atcaccggat acgttaaatc
     1561 ctgcttctct cattaacaga actccccgca tactataata atggactggt atcagggcag
     1621 ataaacctaa gctacagata ctcctaaagg gtacgtcatg tgacgggtat ggttgggact
     1681 cttaagaatc ttcactttgt ccacctccac atcggggtgc taattcagcc ttatctaggg
     1741 gttattccca ctgaaaaggc ggtggaggta ggtgtacggg tataagatgg cgtccatcag
     1801 ggggtgactg gttatcctag ccatagacta tgatccccac cacttccagg tcacggtatg
     1861 ttttttattg gggcctgttt tcaccgagta atcaaggtca aaggtgggtt gtaaaaatct
     1921 gaacttaaga gttgaacctg aaacatctat ggccagtcgt gtaaaagcca cagtggatct
     1981 acgtagaatt aaagagttcc acccccctag ttaatgaaat tagatcaata aagttccaaa
     2041 aactaattta aatattactc cagttactat ggccagggtg atgctgatga atgtacctaa
     2101 caggtagtac tctgcaaagt tcctctcatt taattcattg aatctggcaa ttgatttagc
     2161 agcgaatata atggatatcg ctgcatactg atccgccaaa acgaaggtga agataagtat
     2221 tctttcaaat attcctatta agaatccggc tccctggata ccgctatcct cgatctggga
     2281 gctgtatggt cttagcaata tcctaatgaa cactccgaaa attgccacca ataggtatcc
     2341 cagtgccagg acaagaattt taaacagcat acccacccca caatattttt gaaccactac
     2401 tggatcaact acaaatttga actttatata gttcaatatc taggtttgat ctggtatagg
     2461 atcatttttt tttgaattcc ttttccaaaa ccagttctcc atactttatc aacgaatatt
     2521 tacccacttc taaaattttg tagatattcc ggatcccagt ttcaaagtta tgggcagcta
     2581 gttcaatggc ctggggattt tcaccgtgca gtcgataaaa tgatatgatc tcgctgcgcc
     2641 tgggagtcca actccacatt atatcgaata tatagttgaa gacacattct gttaaatcgt
     2701 tgttcggtaa gttactgttg agtacgatcc atctgttctt ttttttagca gtgttcaatg
     2761 catcttttga aaaatgaaag gcctttccat cgatctcctg gatgtgttca cttaaactgg
     2821 ttgatattga tccctggccc acccccagat aaaaagggtg cctgatcttt ctaaaaaggc
     2881 cgaagtagat atccatgatg atatcaaagg cggatatcat tccctgaaat ccgtctccac
     2941 ctacaatttg aaattcagaa actatgtgct cattaaaatt ggtgttaaga tatttcattg
     3001 cttctttcag attatcctgg gtgatacttc tcttgtcaat tttcttggat gatcgcagat
     3061 caccggtaat tactatgtac tcaagatcac tttctaacat aacagccaac tttcaatggt
     3121 ttttatgatg ttaaaaatcc tttaaatgga tatttagaat ttatagtagt gattatccca
     3181 tttcataatc ataaatgttt ctatagttga aaagtcagcc cataataata tcctgaattg
     3241 tgcagatgac cctctcattt cactaaagat ttccaggaga tttcatggaa attttctaac
     3301 ctttggttct tctttaaaaa gaagggcttt ttccagttat ttttttactg accttccccg
     3361 tgttttgatc ttttcttgga gttcatatga ctatagatcc atgcccattt tacgtagtgg
     3421 gtcccctgat actgatggac catcatagga ttttaggggg ggggttgtct tctaatcaca
     3481 tggatggtta acaagagcca gggattcttc cacctctttt tatgcagtcc atagatttct
     3541 ggctttatca cagcagtcct cattaccagg ttaatgggta tttcctatct tagccttttt
     3601 tttggatcca tggggttaaa taaggaggag gtagagtttt aattgaagac ctggtagggg
     3661 tgtatctgta tgaataaatg gttccctgtg gggttgatat tgctagtggt actcctggtt
     3721 tccatagcct ttctgtacca gagtatgacg gatgaggaga gaaccatcct atcccgggca
     3781 ccggttcata gccaggtgga actggccctg accttctacg atcataacct ggtgagaagg
     3841 gagttataca gtaaatacag ggccaccaat aactatgagt actaccggtc ctatctggac
     3901 tcggggtaca cggtgcaggt tttctctaat ctgtcccacc agaataaact aagcaccgag
     3961 gaggtgaaga tcctagctac caccctgaag gccaacaatg cctactacac ccatcacacc
     4021 tccccacatg gtgatggtat tatcggagcc ttctccaccc agtctcccta ccccaccacc
     4081 aacaccttca ccagcggcaa attcaccagt aaactgccct tcgtctacta ccagggccag
     4141 ggctggcagc tttatccggt caccgccacc ttctgggcct ccgagtactt taaacagggc
     4201 aactacaagg atgggatagc cctcctggat gagttaagtc cctacatgag tgtggaagag
     4261 tatggtggac tggattatgg ggtgttcagg gtctacttcc agtacaccaa ctccagcatc
     4321 ccctgggctt cctcttactc ccagggccag gccgcggggc tctatgccca ggcctacaac
     4381 cacagcaagg atgggaagta tttagaccag tcccatctcc tgttcaactc cttcaaaata
     4441 cccctgagtg aaggtggatt catcacctcc accccatatg ggccctggtt tttggagtac
     4501 aacttcaaac cccggcatct gatacttaac ggacacatca taaccatgca gggaatctac
     4561 cactactacc aggtcacagg gagccaggat gctttggatc tttttaacca gggcgccact
     4621 agtgtgaaga acagcctgcc ccagatggat agtggggact ggagttacta tgccctgact
     4681 ggtgtggatg ccaagccagc ctggaaggcc aacaaatact atatgggcct ccatgtggag
     4741 cttttgaagt ggttacacag taccactggt gactccacct tccagtacta cgcccagagg
     4801 tgggaggggt tttataaaaa taggcaggga gaggtcagca cccccatcat aaccatgccc
     4861 tagactcaga ttcccagtgg acagctcctg ggagtgagta ataataaaac ccccacgttg
     4921 gtaggataaa tggttagacc caccttaaat gagaagggca gtgtcattac agtgaattct
     4981 ggcagggttg ttggcggggc aaaggttgat gctagaaccg gtactttaat tgaagatcgg
     5041 ggataaacaa tattattaat ggggtgtttg tggtggtgaa atgtaccagg tgtggccagg
     5101 aaaatgatga cgacgcagtg tattgtgaag agtgcggatc aaacctatca gagattggtg
     5161 gccaggggcc gcagccagta aaggagggtt ggaatacttc cgccaaggct cttgtcgtgg
     5221 gactggttat cttaatagtg gtcctgg
//
LOCUS       DAOQVW010000037         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_17442_length_5234_cov_3.188067, whole genome shotgun sequence.
ACCESSION   DAOQVW010000037 DAOQVW010000000
VERSION     DAOQVW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17442_length_5234_cov_3.188067"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PKK85_01495"
     CDS             complement(<1..936)
                     /locus_tag="PKK85_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS24730.1"
                     /translation="MECYLISTFAGFIALDENFNLLDYELFPKDKLLEKYLEIQTREI
                     TTEERHLIGRLASSGYQVLIESNNLVSTYKELDYELEFRIESDTRGGKYLRANLPGVL
                     EKIGFIGTASDLREVVHELSIQVTEEKLRTASRADDLLLIQAINAIDDVEESVVKLSE
                     RLREWYPIHFPELDLVKNQEKYVELVVLYGHRDSIVDSGVLIDVLDDNINPETSLGSD
                     ISPEDLEILQKFARSLQSLILTKKSLNTFVDKRMGELAPNLRELAGASLGAKLISHVG
                     SIKGLAMLPSSTIQIMGAEKALFRHLKSGERPPKHG"
     gene            987..1892
                     /locus_tag="PKK85_01500"
     CDS             987..1892
                     /locus_tag="PKK85_01500"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNS24731.1"
                     /translation="MLEVEILNMKLKNPVLLAAGVMGSTASSLNWAARNGVGGVVTKS
                     FSLEPNKGYPNPTTVEVQGGIINAIGLSNPGVEAFKVELGRIDEKVPTIASLYGSSPE
                     EFSLVAKKIEDMVDALELNVSCPHAMTGCGASIGQDPDLTGEMVRAVKKTVNVPVTVK
                     LTPNVTDIVEIALAAQEAGADGLTLINSLGPGMCIDLETARPILSNRFGGMSGPAIKP
                     IALRCVYQAYEAVKIPIIGVGGIMDHKDAVEFLYAGACSIQVGTAVMYQGLEVFQRIC
                     NGLESFMRDKEFNRVDEMVGLAHQD"
     gene            1916..2710
                     /locus_tag="PKK85_01505"
     CDS             1916..2710
                     /locus_tag="PKK85_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNS24732.1"
                     /translation="MHVPRVLEIKRIETENFTVKTFIFDWDQGEVHPGQFLMVWNFQD
                     EKPMSISFTDPVAGEIGVSIKNVGKITDQLHSLIEGDKIGLRGPYGRPFQMVGSKILA
                     VGGGIGMAPVLAFATEAKRRGLDLEVISAAVTEKELLFVEKLEKIGVKVHTATDDGSC
                     GFCGFPTILADNLLQGGDFDMLVSCGPEPMMKGLFEIAENHNIPSQFSLERWMKCAVG
                     ICGQCCVDDVGWRVCAEGPVFWGHELRLIKEFGSYRRDSSGVRHQF"
     gene            2726..3811
                     /locus_tag="PKK85_01510"
     CDS             2726..3811
                     /locus_tag="PKK85_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNS24733.1"
                     /translation="MNYKWKGTITSAFFVVLVLLLLSFLVIYPIASMVILGAIFAYGI
                     RPLALKIEPKLKFRSLAIAVAMVVVILPLIALLAYSINTLISSSSTLVELTRGLDLSL
                     LNSTSVQKSVLLKETIPASIYPYITSILNIVNVELADIIHFIVNYILEIIESIPMLAL
                     QIFIFLASTFYIARDGGKLSEYIQYTIPKGREHYFENLYSEIERVLKSIFYGHFLTAI
                     IIGLMAGIGFYLLGYPYALFLGILTGFFQLIPILGPWPVYTVLTIWDIISGNYIRAVA
                     VLLFGIFLSGSDIYIRPQLSGKYADIHPLIFILGFLSGPLIFGLVGFILGPLILGVAY
                     AAVVAYKNEEELKDVIVDKPAKDDKSQ"
     gene            complement(3879..4292)
                     /gene="hjc"
                     /locus_tag="PKK85_01515"
     CDS             complement(3879..4292)
                     /gene="hjc"
                     /locus_tag="PKK85_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase Hjc"
                     /protein_id="HNS24734.1"
                     /translation="MSKKGSREERELVKMLWDADCAAMRAPASGSATKKPLPDIIAGN
                     GEIYLAIEVKSSSKDRIYINSQKINALLEFSQIFGARPYIGAKFLREKWRFVRPDDLH
                     LTKQENFRLDKELAFSKGLEFDELLGKSQQVKFTR"
     gene            complement(4357..4671)
                     /locus_tag="PKK85_01520"
     CDS             complement(4357..4671)
                     /locus_tag="PKK85_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24735.1"
                     /translation="MIKMEKTCSSIKCDVLCEGKVIGEMDGVIITQWFLKNHYRYTGT
                     FSRFTTKNPKLNRSGITVDIKFRNSKMMAKNASIDWIRGPSGNGTFLAKNLEYNEADE
                     SI"
     gene            complement(4828..4903)
                     /locus_tag="PKK85_01525"
     tRNA            complement(4828..4903)
                     /locus_tag="PKK85_01525"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4867..4869),aa:His,seq:gtg)
     gene            complement(4943..5026)
                     /locus_tag="PKK85_01530"
     tRNA            complement(4943..5026)
                     /locus_tag="PKK85_01530"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4989..4991),aa:Leu,seq:tag)
     gene            complement(5077..5151)
                     /locus_tag="PKK85_01535"
     tRNA            complement(5077..5151)
                     /locus_tag="PKK85_01535"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5113..5115),aa:Glu,seq:ttc)
BASE COUNT         1387 a         1175 c         1200 g         1472 t
ORIGIN      
        1 cccatgcttg gggggccgct ccccgctttt caaatgccgg aatagcgcct tctccgcccc
       61 catgatctgg atggtgctgg aggggagcat ggccaaccct ttgatgctgc ccacatggga
      121 tatcagcttg gcaccaaggg aggcaccggc cagttccctg aggttagggg ccaactcacc
      181 catcctctta tccacaaagg tattcaaaga ttttttggtc aaaatcaggg actgcagact
      241 ccttgcaaat ttctgcagga tttccaggtc ttctggggat atatctgacc ctaaactggt
      301 ttccggattg atattatcat ctaaaacatc aattaatact ccagaatcta caatagaatc
      361 ccgatggcca tacaaaacca ccaattccac gtacttttcc tgattcttaa ccaagtccag
      421 ctccgggaaa tgtataggat accattctct cagcctttcg ctcaacttca ccacagattc
      481 ttccacatca tctatggcgt ttatggcctg gatcaggagc aagtcatcgg cccgggaggc
      541 ggttctgagt ttttcctcgg tgacttggat ggatagttca tgcaccactt ctctcaaatc
      601 actggccgtc ccgatgaagc cgatcttctc caggacaccg gggaggttgg cacgtaaata
      661 cttccccccc ctggtatcag attctatccg gaattctaat tcataatcaa gctctttata
      721 tgtggaaact aaattgttgg actcgatgag gacctggtaa ccactactgg ccagtcttcc
      781 gattagatgc ctttcttcgg tggttatttc cctggtctgg atttccagat acttctctaa
      841 gagtttatcc tttgggaaaa gttcataatc aaggaggttg aaattctcat ctagagctat
      901 aaatccggca aatgtactga tgaggtaaca ttccatggtt ctactgtgta gggggaggat
      961 tataaaacat tgctccgggt gtaatcatgc ttgaagtgga aattctgaat atgaaactga
     1021 aaaacccagt actactagct gccggtgtaa tgggcagcac ggcatcttct ttgaactggg
     1081 cagcccgaaa tggggtggga ggtgtggtta ccaagtcctt tagtctggaa cccaacaagg
     1141 gctatcccaa ccccaccacc gtagaggtgc agggaggaat aatcaatgca attggccttt
     1201 ctaaccctgg agttgaggct ttcaaggtgg aactgggaag aatagatgag aaggtgccta
     1261 ccatagcatc cctgtacgga tcatctcctg aggaattctc tctggttgct aaaaagatag
     1321 aggatatggt ggatgccctg gaattgaacg tgtcctgccc ccatgccatg acgggctgcg
     1381 gtgcatccat aggtcaggat ccggatctta ctggagaaat ggtcagggcg gtcaaaaaaa
     1441 ccgtaaatgt gccggttaca gtcaaattaa ctcccaatgt aacggatatc gttgaaattg
     1501 ccctggccgc ccaggaggca ggtgcggatg gccttactct tatcaactcc ctgggcccgg
     1561 gtatgtgcat cgacctggaa acagcacggc ccattctttc caaccgtttc ggtggaatgt
     1621 ctggaccagc cattaaaccc atagcccttc gatgtgtcta ccaggcctat gaggcagtga
     1681 agatccctat aattggtgtg ggtggcataa tggatcataa agatgcagtg gagttcctgt
     1741 acgccggggc ctgtagcatt caagttggta ccgcagtcat gtaccaggga ttggaagttt
     1801 tccagaggat ctgcaatggc ctggaaagtt tcatgaggga taaagaattc aaccgagtgg
     1861 atgagatggt gggcctggcc catcaagatt aacaatctag ttataggctg ataaaatgca
     1921 tgttccaagg gttctggaga taaaaaggat agaaactgaa aatttcaccg ttaaaacttt
     1981 catatttgat tgggatcagg gggaggttca tcccggacag tttctgatgg tctggaactt
     2041 ccaggatgag aagcccatgt ccatttcttt cactgatcca gtggctggtg agataggcgt
     2101 atccatcaag aatgtgggga aaatcaccga ccagttgcat tccttaatag aaggtgataa
     2161 aattgggctg cgtggcccct atggtaggcc attccagatg gttggatcaa agatactggc
     2221 tgtgggggga ggtattggaa tggccccggt cttggccttc gccaccgaag ccaagaggag
     2281 gggtctggac ttggaggtca tcagcgctgc agtcaccgaa aaagagttac tttttgttga
     2341 aaaacttgag aaaatagggg ttaaggttca caccgccact gatgatggta gttgtggttt
     2401 ctgtggtttc cctacgattc tagctgataa tcttctccag ggtggtgact ttgatatgct
     2461 ggttagttgt ggtccggagc ccatgatgaa ggggctgttt gaaattgctg aaaatcataa
     2521 tatcccctca cagttctcac tggagcgttg gatgaaatgt gccgtgggaa tatgtggcca
     2581 gtgctgtgtg gatgatgtgg gatggcgagt atgtgccgag ggaccagttt tttgggggca
     2641 tgagttgcgg ctgataaagg aattcggatc ctaccggcgt gactcatcgg gtgtaaggca
     2701 ccaattctaa actaagtggt gtatcatgaa ttacaaatgg aagggcacca tcacttcggc
     2761 atttttcgtg gtcctggtac ttcttttact atctttcctg gtgatctacc ccatagccag
     2821 catggttatt ttgggggcta tctttgccta tggtataagg ccactggcct tgaaaataga
     2881 acctaaactt aaatttcgtt ccttggccat tgctgtggcc atggttgtag tgatcctgcc
     2941 cttaattgca cttttagcct acagcatcaa cacccttatc tcctcctctt cgacactggt
     3001 agaacttacc cgaggcctag atctcagttt actcaacagc acctcggtcc agaagagtgt
     3061 tttacttaag gaaactatcc ctgcatccat ttatccctac ataacatcta ttctcaacat
     3121 cgtcaacgtg gaactggcgg acatcatcca ctttatcgtt aactacatcc tagaaattat
     3181 agagtccatc cccatgctgg cccttcagat attcatattc ttggcatcaa ctttctacat
     3241 agcgcgagat ggtggaaaat taagcgaata catacagtac actataccaa aaggccgaga
     3301 acactacttt gaaaatttgt acagtgagat cgaacgggtt ctcaagagta tcttctacgg
     3361 acatttctta actgccataa taataggatt aatggcggga ataggatttt atctccttgg
     3421 ttatccatat gctttgtttc taggtatttt aactggattt ttccagctaa ttcccatctt
     3481 agggccttgg ccggtgtaca ccgtactcac tatctgggat attatatctg gtaattacat
     3541 ccgtgcagtg gctgtactac tctttggaat attcctgagt ggcagtgaca tctacatccg
     3601 cccccaactt tcagggaaat acgcggacat ccaccccttg atatttattc ttggcttcct
     3661 atccgggccc ctgatattcg gattagtggg gttcatactg ggacccctga ttcttggagt
     3721 ggcgtacgct gcagtggtgg cctacaaaaa tgaagaagaa ctgaaggatg tgattgtaga
     3781 taaaccagcc aaggatgata aaagccaatg agggttattt attgaggttt gatggatagc
     3841 tactctcatt ccaggaacaa tgaattggaa aatgtcactt atctggtaaa ttttacttgc
     3901 tgacttttac ccaatagttc atcaaattcc agacccttgg aaaaggctaa ctccttatcc
     3961 aaacggaagt tttcctgctt ggtcaggtgg agatcatcag gccggacaaa acgccatttt
     4021 tcccttaaaa acttggctcc gatgtaaggt cgggccccga aaatctgtga aaattccaat
     4081 aaagcattta ttttctgaga attaatataa atacggtctt ttgaagatga tttgacttca
     4141 attgctaaat aaatttcacc attaccagct ataatatctg ggagaggttt cttggtggcg
     4201 cttcccgatg ctggcgccct catagcagcg caatccgcat cccataacat tttaacgagt
     4261 tcacgctctt ctctggaccc ttttttgctc atgctacacc aaaatcaata aatagatgga
     4321 gctgatgata aaatcaggta ataaattttc aaaactttaa atagattcat cagcttcatt
     4381 atattctaag tttttggcaa gaaaagttcc atttccactg ggacccctta tccaatctat
     4441 tgaagcattt tttgccatca ttttactgtt tctgaatttt atgtctactg ttatgccgga
     4501 acgatttaat ttaggatttt tagtggtgaa tctagaaaaa gtcccggtgt accggtaatg
     4561 attttttaaa aaccattggg ttataattac tccatccatc tctccaataa ctttcccctc
     4621 acacaaaaca tcgcacttta ttgaactaca tgtcttttcc atttttatca tgaaacccac
     4681 caacctctag ttccatgcca taatatgaaa cattgggaat aagtagtttt cgaattttag
     4741 caataatacg tgactattga tccatttcta cttggaaaaa ggattcatag aaaataggat
     4801 tccaataaaa cataaggaag ataaaaatgg ggccggggcc gagattcgaa cccgagtcgc
     4861 gggatccaca gtcccgtagg ataaccacct accccacccc ggcactttga ttggtcatag
     4921 ttgtggatag tgacatgtgc gatgcagggg cagggattcg aaccctggta ggcctacgcc
     4981 aacaggtcct aagcctgtcc cctttggcca gctcgggcac ccctgccgta tcataaactg
     5041 taaacatctt gtgcgatgta tttgaaaata ttaaaatgct ccggccggga ttcgaacccg
     5101 agtcatcggc tcgaaaggcc gacatgattg gccggactac actaccggag caaatgaaac
     5161 tatatttctc caccaaaact cttaacgaat tttgaatgga atctgaagtg ggcccaatgg
     5221 gattcgaacc catg
//
LOCUS       DAOQVW010000038         5199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_17607_length_5199_cov_2.669712, whole genome shotgun sequence.
ACCESSION   DAOQVW010000038 DAOQVW010000000
VERSION     DAOQVW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17607_length_5199_cov_2.669712"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="wecB"
                     /locus_tag="PKK85_01540"
     CDS             complement(<1..961)
                     /gene="wecB"
                     /locus_tag="PKK85_01540"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNS24736.1"
                     /translation="MKIAFVIGTRPEIIKMSPLIDEVKHRGINYILVHTGQHYDHEMS
                     QQFFTDLELEKPDYNIGVGSGAHGEQTSMMISRIEEVLLEEKPDLVLVEGDTNAVLAG
                     ALVAAKLHIPVGHVEAGLRSYDKTMPEEINRMVADVCTQLFFVPTLTTATNLLLEGVS
                     PKNIFITGNTVVDACQRNLKIARKHSNLAGEIDPRANVLTLTLHRAENVDHPWRLSQI
                     VNSLLELDDLTIIFPVHPRTAKNLHKFGLYRKLDEAPHVQIIKPMGYLEFLLLLSESH
                     LVMTDSGGIQEEAITLNLPCITLRYNTERPETVEAGGNILVGTT"
     gene            complement(1015..2370)
                     /locus_tag="PKK85_01545"
     CDS             complement(1015..2370)
                     /locus_tag="PKK85_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HNS24737.1"
                     /translation="MKGLIVGFDPGHTVGVAIMDLNGQVLSVKSFKEASRSDVVSHII
                     SYGKAVLISTDVYPPPKMVKKLSSILKSRIDAPSRFMSVESKIEAVDLYLNEKSSFPK
                     SKVVPSDTIPQNAHERDALAAALKTYKKYQNKLQQITKKADELDLTSFEVDTVKIMFL
                     QGSPVSKAIDHILETRKSKKTQLDIEHTPELQLKLNRSSSKDEYDSLGYLRHISRLKQ
                     RIKSQETEIKNLKKLNLKMEEKLEKAQQEASTLNEKIEDLHYQYSKGILAKKELTSKI
                     AIIKRLQAKYQHEKELKQNLQENLNSIKNIQALELSKNVVPAKIIESFTREGIKEACD
                     YWKIKEGDVVFLKNSKGGGSQTASLIIQRGIKALIINDQMSHTAEEEFEKNMIPLIPL
                     ETVDLKMIDKFAVVSVDSLRTQIESWKAQVKDRKASEDRKKLLSVIDEYRAKRRRSVD
                     S"
     gene            complement(2541..3401)
                     /gene="truA"
                     /locus_tag="PKK85_01550"
     CDS             complement(2541..3401)
                     /gene="truA"
                     /locus_tag="PKK85_01550"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNS24738.1"
                     /translation="MLKIALKIAYLGCDYYGFARQPDLPTVEGELIKALTRSEIIDNP
                     SDAGYSIAGRTDRGVHALGNVISFRTESMINVNQINDCLPASIKVLAQTSVHHGFKPR
                     FARQRHYRYLMPKNPFEENWDMKSMKEAAKLFIGTHDFSNFTKRSERDPVRTVNNRVV
                     RNQDHVICFDIKGESFLWNMVRKMVTAIINVGLGKIEVRDVAKHLNPQRKVFITPAPA
                     EGLILMDVTYDTVKFVEDPYARRLFLSVLMKNYMNTCIHSAAYREMMGSLDNNEKCIL
                     PLYNELKVLK"
     gene            3574..4515
                     /locus_tag="PKK85_01555"
     CDS             3574..4515
                     /locus_tag="PKK85_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNS24739.1"
                     /translation="MNRIWGYISVMMAMIFFGISNTFNKILLQTLDPIDLAALSYCLA
                     AVFLFLARYSPLKNRLSSFLDTGHDLEHFMSPKDYLILFSTAILGILIAPLLYLNGLK
                     YSTAVNASFLLIVEILFIILIGILFLKESFQRKDILAFFLLLLGTVLLLTGGNLENLF
                     MSYSWGNMLIILAAFCWSIDTTLSKFLSFKSDIVLITALKSAIGGLLLLSLSAYMGLK
                     IQMSLTYLPYLLFVGIFSIGTALILIYLSIRIIGATRAGSLFSLASLFGAVSAFFILK
                     EPFTIMQLFFGLLMLAGVLIFYLNGEQGTGDLKKMAR"
     gene            4624..>5199
                     /gene="hisA"
                     /locus_tag="PKK85_01560"
     CDS             4624..>5199
                     /gene="hisA"
                     /locus_tag="PKK85_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNS24740.1"
                     /translation="MVIPAVDIKNGKCVQLVQGKPGTEQVILEDPVKIACQWEDKGAR
                     MIHVIDLGGALGETNNLEIVREIRDSVSVPIQMGGGIRTKDDALNLLEMDINRIILGT
                     IAMENPEVVPELSNSFGKKKIVVALDSKDSQVVVRGWTEKTDKTAPEMGKFFENMGAG
                     GILFTNVDYEGLMKGFSIKPLEELLSAVKIPV"
BASE COUNT         1472 a         1016 c          988 g         1723 t
ORIGIN      
        1 gggttgttcc caccaatatg ttcccaccag cttccactgt ttcaggtcgt tcggtgttgt
       61 atcgtagggt tatacagggt aagttaagag ttattgcttc ttcttgaatt ccacccgaat
      121 cagtcatcac cagatgagac tcggacaaga gtagtaaaaa ttccaggtac cccattggct
      181 taataatctg tacatggggc gcttcatcca gtttcctata caaaccaaat ttatggagat
      241 tcttggcggt acggggatgt accgggaaga ttatagttaa atcatccagt tctaacagag
      301 aattaacaat ctggctcagc ctccaggggt ggtcgacatt ttcagcccgg tgcagagtca
      361 gagttaaaac gttggccctg ggatcgattt ctcctgccaa gttagaatgt ttcctggcaa
      421 ttttaagatt acgctgacag gcatccacca ccgtgttacc ggtgatgaag atgtttttgg
      481 gactaacacc ttccaataat agattagttg ctgtagtgag ggtgggtaca aaaaatagtt
      541 gggtgcagac atcagccacc atccggttta tctcttccgg catggtttta tcataagaac
      601 gtaagcctgc ctctacatga ccaacaggaa tatgaagttt agctgccacc agtgcaccag
      661 ccagaaccgc gttggtatct ccttctacca gaactagatc tggtttttct tcaaggagaa
      721 cttcctcgat acgactaatc atcatggagg tctgttctcc atgggctcct gatccaaccc
      781 caatattgta atcgggtttt tccagttcca ggtccgtgaa aaactgctga ctcatctcat
      841 gatcataatg ctgtccagtg tgtactaaaa tgtagttaat tccccggtgc ttcacctcat
      901 caatgagggg tgacatttta atgatctctg gtctggtacc aatgacaaaa gcaatcttca
      961 ttttaaacac ctaaactatt catttaagaa aatacattga tatcgcagga tactttatga
     1021 atcaactgat ctccttctct tagcacgata ttcatcaata acacttaaaa gttttttcct
     1081 gtcttcactg gccttcctat cctttacttg ggctttccag ctttcaattt gggttcgaag
     1141 tgaatcaaca ctgaccactg caaatttgtc aatcatcttc agatcaaccg tttccagggg
     1201 gatgagggga atcatatttt tctcaaattc ttcttcagca gtatgcgaca tctgatcatt
     1261 aattattaag gcctttatac ccctctggat tattaaagaa gcagtttggg atcctccacc
     1321 tttagaattc ttaaggaaaa ccacatctcc ctcttttatc ttccagtagt cacaagcttc
     1381 ttttatgccc tcccgagtga aggattctat tattttagct ggcaccacat ttttagaaag
     1441 ttccagggcc tgaatatttt tgatagaatt taaattttcc tgcaaattct gctttaattc
     1501 cttttcatgt tgatatttag cctgtaatct ttttataata gcaattttgg atgttaactc
     1561 tttttttgcc agaattcctt tggagtactg gtaatggagg tcttcaattt tttcattcaa
     1621 agtcgaggcc tcctgctgag ctttctccaa cttttcttcc attttaaggt tcagtttttt
     1681 taaattttta atttcagttt cctgagattt aattctctgt ttcaatcgac taatgtgcct
     1741 caaataccct aatgaatcat attcatcttt agaagatgat ctattaagtt ttaattgtaa
     1801 ttcaggagta tgttcaatgt ctaattgagt tttcttgctt tttctggttt ctaaaatatg
     1861 atctatggct ttactaacag gtgacccttg taaaaacatg attttaactg tatccacttc
     1921 aaatgaagtt aaatccaatt catcagcttt tttagtgatt tgttgtagtt tgttctggta
     1981 ttttttgtaa gtttttaaag ctgcagccag tgcatctctt tcatgagcat tctggggtat
     2041 tgtatcagat ggcaccactt tagatttggg aaaagaagat ttttcattta aataaagatc
     2101 cactgcttca atttttgatt caaccgacat gaatcttgag ggagcatcga tacgcgactt
     2161 caaaatagaa gaaagtttct taaccatctt aggaggagga tatacatcgg ttgatatcaa
     2221 gactgcttta ccataactaa ttatatgact gacaacatcg gatcggctgg cttccttaaa
     2281 gcttttaact gataagacct ggccattaag atccataatg gccactccta cggtgtgtcc
     2341 cggatcaaaa ccaacaatta gccctttaat attttttaag tccttataat ccccatatga
     2401 agagttaaag tttttatcat ctctgcccct ataaaacaga ctttttcctc ctgggtaaat
     2461 ttaattccca aataataata tggttttcat attatttaat tgtttgtcag tcccagaatg
     2521 atgccaattc atcttcaata ctattttaaa acttttaatt cattatataa agggaggata
     2581 catttttcat tattatcaag actccccatc atctctcggt aagcagccga atgaatacag
     2641 gtattcatat agtttttcat caaaaccgac aggaataacc tccgggcata aggatcctct
     2701 acaaatttta cagtatcata ggttacatcc atcaagatta agccttcagc aggtgcggga
     2761 gtgatgaata cttttctttg tggatttaaa tgtttagcta catctctgac ttcaattttt
     2821 ccaaggccga catttattat ggccgtcacc atcttacgta ccatattcca cagaaaactc
     2881 tcacccttaa tatcaaaaca tattacatgg tcctgattcc tcaccacccg gttgttgacg
     2941 gttcgaaccg gatctctctc acttcttttt gtaaaattac tgaagtcatg agtaccaata
     3001 aataatttgg cagcttcttt catacttttc atgtcccaat tttcttcgaa gggattcttg
     3061 ggcattaaat aacggtaatg acgttgtcgg gcaaatctag gtttgaatcc atggtgcaca
     3121 ctggtttggg ccaggacctt tatagaagca ggtaaacagt cgtttatttg atttacattg
     3181 atcatagatt cagtgcgaaa tgaaatcaca tttcccaggg catgaacccc acggtctgtt
     3241 cggccagcta tggagtaacc agcatcggaa ggattatcaa ttatttctga tcttgtgagg
     3301 gctttgatca gttcaccttc cacagtaggc aggtcaggtt gtcttgcgaa cccgtagtag
     3361 tcacatccca aataggctat ttttaatgca attttcaaca tattatgcat tctcgattta
     3421 tgtaaagttc tctgccccgt tggaacagaa attatcataa agatttctag actaagtaat
     3481 taagtttcta cccactaatt ctatataagc tatcagatga caagaaggta aatatccaag
     3541 actggatttg gctaattttt atgaggtaat tgaatgaatc gtatatgggg atatatcagt
     3601 gtcatgatgg ccatgatttt ttttggtatt tccaatacct tcaacaagat attgctgcag
     3661 actctggacc ctatagactt agctgcactt tcttactgtt tggcggctgt ttttttgttc
     3721 ttggcacgtt attcacccct aaaaaacaga ttatcttcat ttctggacac aggacatgat
     3781 ctggagcatt tcatgagccc caaggactac cttatcctat tcagcaccgc cattttgggg
     3841 atattaatag cccccctgct ttatctaaat ggtttaaagt acagcacagc agtaaatgct
     3901 tcgtttcttt taattgtgga gatcctgttt ataatcttaa tcggaatttt attcctcaag
     3961 gagagtttcc agagaaaaga tatattggct ttttttcttt tactactggg aacagttctt
     4021 cttttgaccg gaggcaattt agaaaatctc tttatgagtt acagttgggg aaatatgtta
     4081 atcattttgg ccgcgttttg ctggagcata gacaccactt taagcaaatt tttgagcttc
     4141 aagagtgata ttgtcctcat taccgcctta aaatcagcta ttgggggttt gctcctactt
     4201 agcttatcag cttacatggg cttaaaaatc cagatgtccc tgacttattt accctaccta
     4261 ctctttgtag gtatattcag tattgggact gcgttaatcc ttatttatct ttccattagg
     4321 attatcggtg ctacacgggc tggttctttg ttttcactgg cctcactatt tggagccgtg
     4381 tctgcctttt tcatcctaaa agaacctttt acaattatgc aacttttctt cggactttta
     4441 atgttagcag gagtgttaat tttctatctc aacggtgaac aaggaacagg agatttaaag
     4501 aaaatggcac gataattaac ttgcaaggta tgtcataaaa aaatgttatt ggcaccactg
     4561 gttaatatga tataatataa gatggtttag accataacaa tctcgttaag gtgaaaaaca
     4621 gtgatggtta ttcctgcagt tgatattaaa aatgggaaat gtgtacagtt ggtccaggga
     4681 aaacctggaa cagaacaagt tattctggaa gacccagtga aaattgcctg tcaatgggag
     4741 gataaagggg cccggatgat ccatgtcatc gatttgggag gggcactggg tgagaccaat
     4801 aacctggaaa ttgtaagaga aattagagat tctgtatctg tacctataca gatgggtgga
     4861 gggataagaa ccaaggatga tgctttaaat ctccttgaga tggatataaa taggataatt
     4921 ctgggaacaa ttgccatgga aaatccggaa gtggtacctg aactttccaa cagtttcgga
     4981 aaaaaaaaga ttgtggtggc actggatagt aaggattccc aagtggtggt tcggggttgg
     5041 actgaaaaaa cagataagac cgctccagag atgggtaaat tctttgaaaa tatgggagca
     5101 ggaggaatct tatttaccaa tgttgattat gaaggcctta tgaagggatt cagtataaaa
     5161 ccactggaag aattactcag tgcagtaaaa atccctgta
//
LOCUS       DAOQVW010000039         5138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_17884_length_5138_cov_2.825300, whole genome shotgun sequence.
ACCESSION   DAOQVW010000039 DAOQVW010000000
VERSION     DAOQVW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17884_length_5138_cov_2.825300"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKK85_01565"
     CDS             complement(<1..426)
                     /locus_tag="PKK85_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNS24741.1"
                     /translation="MVVTIKSSEFNEGEPIPPRYTCDDLDISPPLEWHSPHEGGSWAL
                     ICDDPDAPNGLWTHWVLFNLPPQTRSLAEHVINQEELENGARQGVNSWGNIGYQGPCP
                     PQGVHHYIFKIYLLDTRLDLPSSATRQQVLEAMEGCLLDE"
     gene            698..907
                     /locus_tag="PKK85_01570"
     CDS             698..907
                     /locus_tag="PKK85_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HNS24742.1"
                     /translation="MTEIPIAPVGRIIKNAGAQRVSDEAREELTKILEAYGEDIAFEA
                     VKMAKHARRKTVKASDIEMALKVLM"
     gene            981..1277
                     /locus_tag="PKK85_01575"
     CDS             981..1277
                     /locus_tag="PKK85_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24743.1"
                     /translation="MEFKEKIKLVLIAALVVAGVAAVIYLAYGLTIIWQGMAAGVTVG
                     FLLVLLLAVVILAIYFWIRIFFTKRELKKCQARVEELEHRLQSESQVKKPEDEP"
     gene            1434..3209
                     /locus_tag="PKK85_01580"
     CDS             1434..3209
                     /locus_tag="PKK85_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HNS24744.1"
                     /translation="MTRIAILDQDRCQPKKCHYTCMEYCPGVRMEEDTITIDQKSKKP
                     LISEELCSGCGICTNRCPFRAVSIINLPEALEEPIHRYGANQFELFGLPQVKEGSVVG
                     MLGPNGIGKSTILRILSRELKPNLGNYDKESSWEDIVKFFKGSQMQNYFQKLAQEKIR
                     VVHKPQMVDMLPRFVEGKVHQLLESVNQRQRLEEVVEALQLESVMNREIINLSGGELQ
                     RVAIAAAVLREADFYYFDEPTSWLDVQQRLNAVQVVRQLAEDGKSVLVIEHDLAALDA
                     LSDYVHLLYGQPGAYGVVSHLRGVRVGINAYINGFLKEENVRFRKQPLEFQVRPPTLG
                     VEKEVLASYTHLEKSFEGFSLDVEEGEVQHDEIITAFGPNGIGKTTFAKMLAGVIKPD
                     AGKIQKNVKISYKPQYLVSDYSGTVKEFLYTTVPSFGSNIFRTEIMKPFLLEELLEKK
                     MDELSGGELQRLSLAISLSQEAEVYLFDEPTAHLDVEQRIRAAKAIRRVTEGKNAAAI
                     IVDHDIVFIDYISQRAMVFSGKPGVEGCASSPMDLRSAMNRFLSEVGITFRRDKETKR
                     PRVNKKDSYLDREQKEKGEYYYLES"
     gene            3325..4962
                     /locus_tag="PKK85_01585"
     CDS             3325..4962
                     /locus_tag="PKK85_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNS24745.1"
                     /translation="MASAYQSNLKRLHVIIRILYKYEFGYIIEKIKLKHKIPFIRRSY
                     KYESLEELDESTPERIRFVLQELGPTFIKLGQTLSTRPDLVGERMALEFTKLQEDNPP
                     LEFDVIKTTLEEELGSALETIFESFDPEPLGSASIGQVHRAVLKSGEEVAVKIQKPGV
                     EGIIKNDIAIMLFLARRINSYIPKLRIYNLPGIVEEFERSILKEIDYQNEAMNLKRFR
                     QNFQMDHTVYVPQIYSQHSTPKVITMELIQGRKVSEVTEADGYHLKLIAKRGALSYFK
                     QVVEDGFFHADPHPSNIYILKGDVLCYLDFGMMGVLDEDFRQDLAELFIYLMDTNVKG
                     TISKLQQMGIIDEHVDTRSLKYDLIDLLFKYYGTELSEVHGGLDDLLRLMRKYQATLP
                     RELVLLARGIGMVEEMGRKLDPTFNAVEIAQPLVRKIARKRLSPLNFLDYLKKNIFEM
                     EHTMKNMPTSITRTLYRLEKGDMQIKVVHSGLERSTNKLSISLVLAALLIGSSLIMTT
                     DNGIFLLKFPYLGVAGFIISAILASGLVLSILRDQGI"
BASE COUNT         1346 a         1211 c         1352 g         1229 t
ORIGIN      
        1 ctcatctagc aggcagccct ccatggcctc cagcacttgt tggcgggtgg cagaggatgg
       61 cagatccagc cgggtgtcca ggaggtagat tttgaagatg tagtggtgca ctccctgggg
      121 agggcagggg ccctggtatc caatgttacc ccaactatta acaccctgtc gtgccccgtt
      181 ttccagttcc tcctggttaa tcacatgttc tgctaggctc cgggtctggg ggggaaggtt
      241 aaaaagaacc cagtgggtcc ataggccatt gggggcgtcg gggtcgtcac agatgagggc
      301 ccagcttcca ccctcgtggg gggaatgcca ttccaggggt ggtgaaatat ccagatcatc
      361 acaggtataa cggggaggta ttggttcccc ttcattaaat tccgagcttt ttatggttac
      421 aaccatctaa atctcctcgt attccatttt ttaataggga tagtacatct tatgttgctt
      481 ttggtactta taaagaggtg gaagtcagat actttgggga ttgatctgga gaagttacgt
      541 agccagaggc cctattggag tgaaaaaata agtaccaacc cctatggtgt aatgtgattt
      601 tcaagatgga catgtttgga ttaaccacgg attaataacc aaaaaaacaa catacttaaa
      661 tactagatgg tcctaataga acaatgaggt ggttttaatg accgaaatac caattgctcc
      721 tgtaggaaga ataataaaaa atgctggcgc acagagagtc agtgacgaag ccagagaaga
      781 attaaccaaa atactggaag cctacggtga agatattgct tttgaagctg ttaaaatggc
      841 taagcacgct cgtagaaaaa cagtgaaagc atcagacatt gaaatggctc ttaaagtcct
      901 gatgtaactt tcttcttttt ttattttagt ttttaattct tttaaagcgt atttatctga
      961 cagttaggta ggttgtatct gtggagttca aggagaagat caagttagtg ctgatcgctg
     1021 ccttggtggt ggctggagtg gccgccgtga tctacctggc ctacggtctc accatcatct
     1081 ggcaggggat ggccgccggg gtaactgtgg ggttcctact ggtactccta ttggcagtgg
     1141 tcatactggc catctacttc tggatcagaa tattcttcac caagagggaa cttaaaaagt
     1201 gtcaggcccg agtggaagag ctggaacaca gattacaatc tgaatcccag gtaaaaaaac
     1261 cggaggatga accataaggt tcttcatccg atgccccatt ttttttcata aaccccgatc
     1321 ccccaactaa tattaaatgg aagcttccaa tctaaatcta catttaaata ttataatccc
     1381 atttctacca aaggcaaaca agcctttcct aataagttcg aggttttgaa ttcatgaccc
     1441 gtatagccat actagaccag gatcgctgcc agcccaagaa gtgccactac acttgcatgg
     1501 agtactgccc cggggttcgc atggaagaag acaccatcac cattgatcaa aagtccaaga
     1561 aaccccttat atccgaggaa ctgtgctcgg gttgtgggat ctgcaccaac cgctgtccct
     1621 tcagggcggt gagtattatt aacctccctg aggccctgga agagcccatc caccgttatg
     1681 gtgccaacca gtttgaactc tttggtctgc cccaagtgaa ggagggctcg gtggtgggta
     1741 tgctgggccc caatggtatt ggcaaatcca ccatactccg catcctctcc agggagctta
     1801 aacccaacct gggaaactac gacaaggaga gttcctggga ggatattgtg aagtttttca
     1861 agggcagtca gatgcagaac tacttccaga aactggccca ggaaaagatc cgggtggtgc
     1921 ataagcccca gatggttgac atgctacccc gcttcgtgga gggtaaagtg catcagttac
     1981 tggagagtgt gaaccagcgc cagcgcctgg aggaggtggt ggaggctctg cagctggagt
     2041 cagttatgaa ccgggagatc atcaacctaa gtgggggaga actgcaacga gtggccattg
     2101 ccgctgcggt gctccgggaa gctgattttt actacttcga tgagcccacc agttggttgg
     2161 atgtccagca acgcctgaac gcagtgcagg tagtgcggca gctggccgag gatggtaagt
     2221 ctgtcctggt tatcgaacac gacttggctg ccctggatgc cctctcggac tacgtgcacc
     2281 tgctctatgg tcagcccggc gcctatgggg tggtttccca tctgcggggt gttagggtgg
     2341 gtatcaatgc ctacatcaac ggattcctca aggaggagaa tgtacgcttc cgtaagcagc
     2401 ccctggaatt ccaggtgagg ccacctactt taggggtgga aaaagaggtg ctggcctcct
     2461 acacccacct ggaaaaatcc tttgagggtt tttccctgga tgtggaggag ggtgaggtgc
     2521 agcacgatga gatcatcact gccttcggcc ccaatggtat agggaagacc accttcgcca
     2581 agatgttggc cggggtgatt aagcccgatg caggtaagat ccagaagaat gtgaagatat
     2641 cctacaaacc acagtacctg gtctctgatt acagtggcac ggtgaaggag ttcctctaca
     2701 ccacggtgcc tagcttcgga agcaacatct tccgcaccga gataatgaag cccttcctct
     2761 tggaggagct cctggagaag aagatggatg aactcagtgg gggcgaactg caacgactgt
     2821 ccctggccat ctccctatcc caggaggccg aggtgtacct cttcgacgag cccaccgccc
     2881 acctggacgt ggagcagcgg atccgggctg ccaaagccat ccgtcgggtt accgagggta
     2941 agaatgctgc ggctatcatc gtggaccacg acatcgtgtt catcgactac atcagccagc
     3001 gggccatggt cttctctgga aaaccaggag tggagggctg tgcctccagt cccatggatc
     3061 tgagatcagc catgaaccgc ttcctatccg aggtgggcat caccttccgc cgggataagg
     3121 agactaaacg tccccgggtg aacaaaaaag acagttacct agaccgggag cagaaggaga
     3181 agggggagta ttattacctg gagagttaag tagaggaaaa tgccattatc tacaactggc
     3241 tttccaggta ttattcagtg ccacgtgtag ttaggggact taaagaaaga cctagggaat
     3301 agtttaaatc taggaggaat tgctgtggca tcagcatatc aatccaatct taaaagactg
     3361 catgtaataa ttcggatcct gtacaagtac gagtttggct atataatcga gaagataaag
     3421 cttaaacata aaataccatt tatcaggcgt tcctacaagt acgaatcatt ggaggagttg
     3481 gatgaatcca cgccagaaag gatcaggttc gttttacagg agctgggccc caccttcatt
     3541 aaactgggcc agaccctcag cacccggccg gacctggtgg gggagaggat ggccctggaa
     3601 ttcaccaaac tccaggagga taatccaccc cttgaatttg atgtcataaa gacaaccctg
     3661 gaggaagagt taggatcggc cttggaaacc atttttgaaa gttttgatcc tgaacccctg
     3721 ggttctgctt ccattggcca ggtgcaccgg gctgtgctta agagtgggga agaagtggcg
     3781 gtgaagatcc agaaacccgg cgtggaaggg attatcaaaa atgatattgc catcatgctg
     3841 tttctggccc ggagaatcaa cagttacatc cccaagttgc ggatctacaa cctcccgggt
     3901 atcgtggagg aatttgaacg ttccatcctc aaggagattg actaccaaaa tgaggccatg
     3961 aacctgaaac ggttcaggca gaacttccag atggatcata ccgtgtacgt cccccagatc
     4021 tacagtcaac actccacccc taaggtcatc accatggaac tcatccaggg ccggaaagtc
     4081 tctgaggtca cagaggcaga tggctaccat ctgaagttaa tcgccaaaag aggggcttta
     4141 tcctatttta aacaggtggt ggaagatggt ttcttccacg ccgaccccca cccctctaac
     4201 atatacattt taaaaggaga cgtgttgtgt tacctggact tcgggatgat gggtgtgctg
     4261 gatgaggatt tccgccagga tttggcggaa cttttcatat acttaatgga caccaatgtt
     4321 aagggtacca tcagcaaact ccagcagatg ggaatcatag atgaacacgt ggacacccgg
     4381 tccttgaagt acgacctcat cgacctcctc ttcaagtact acggcaccga actcagcgaa
     4441 gtgcatggtg gtctggacga cttgctacgg ctcatgagaa agtaccaggc aacccttccc
     4501 cgggagctgg tgctcctggc ccgtggtatt ggcatggtgg aggagatggg aagaaagctg
     4561 gatcccacct tcaatgccgt ggagatcgcc cagcccctgg tcaggaagat agcccgtaaa
     4621 agactgagtc ccctgaactt cctggattac ttgaagaaga acatattcga gatggaacat
     4681 accatgaaaa acatgcccac cagtattacc agaactcttt atagactgga aaaaggggat
     4741 atgcagataa aggtggtgca ctccggcctg gaaagaagta ccaacaagtt atcaatatca
     4801 ctggtgctgg ccgcccttct cattggttct tccctcatca tgaccactga taatggtata
     4861 ttcctcctta aatttcccta cctgggggtg gcagggttca taatcagcgc catcctagct
     4921 tcgggtcttg ttttgtccat cctccgggac caggggatct gaccaggacc ctgcttgata
     4981 gcaatcctta gctttaaggg gggtggtagt ttgactgctg tgatccttgt cctagtagac
     5041 cactctggta gagccatctg gttctatctt ctctgacgga tcttagcacc tggaagtggg
     5101 tagtgctcca gaaggacaga agataaatgg agttagag
//
LOCUS       DAOQVW010000040         5124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_17949_length_5124_cov_2.975932, whole genome shotgun sequence.
ACCESSION   DAOQVW010000040 DAOQVW010000000
VERSION     DAOQVW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17949_length_5124_cov_2.975932"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..819
                     /locus_tag="PKK85_01590"
     CDS             283..819
                     /locus_tag="PKK85_01590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS24746.1"
                     /translation="MINVVYDIKIYREVLRDIIKQDDLVVELGCHLGNSTKIMAERAP
                     KGRIITMDKSEEAAQAMSELKSFHHNVEFLKADVRLHETLEKVARMLKDHGGCDVLSI
                     DLGGGHYPDTTFKVFFIWSSTLRPRETIIRNRGLLDFMHSSSSSEIIKSSEGWLESSG
                     GDGVPPRLKELQLWSTNI"
     gene            834..1628
                     /locus_tag="PKK85_01595"
     CDS             834..1628
                     /locus_tag="PKK85_01595"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HNS24747.1"
                     /translation="MIGKRIRIERILDRKTGRCVIVPMDHGVSIGPVPGIIDMAHTID
                     EAASGGANAVIMHKGMVGSGHRGYGRDIGLIIHLSASTGLGPDPNHKVLVTSVEKAIQ
                     MGADAVSVHVNVGSKREPEMLMQLGSIAEICDDWGMPLIAMMYPRGKKIENEHNVEVV
                     KLASRAGAELGADIIKTNYTGDVDTFQEVVEGCPVPLVIAGGPRVETDRELLDMVKNS
                     VDVGGAGVAIGRNIFQAKSPRKTTQAIAEIVHNNLDVDEALEILKG"
     gene            1646..2770
                     /locus_tag="PKK85_01600"
     CDS             1646..2770
                     /locus_tag="PKK85_01600"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HNS24748.1"
                     /translation="MKFAWIMAEGTDWNQKKTFITAALESGMDHVVDFTDVDRIRKLG
                     NITIISDTEGSDIQLVGRNGEGDSTLALPSELSESKDLAAVRHLKKRGKTTAAYVEIT
                     SKRHEELASLLGREADYLILLGRDWKVIPLENIIADLQKEKVKLIASVADYEEAKLSL
                     ETLEYGTDGVMLSPREVGEIKKVADLLEKIEHQSYPLKVATVTRVEPVGSGDRVCVDT
                     CSMMSLGEGMLVGSYSQGLFLVHSESLESEYVASRPFRVNAGPVHAYVMAPGNRTRYL
                     SEVQTGDEILAVDKDGNSRNVIVGRVKIEKRPLMLVEAEADGEVLRTLLQNAETIRLV
                     GEDGDPISVADLKAGHKIMVYLDESARHFGMAIEESIIEK"
     gene            2916..3467
                     /gene="thpR"
                     /locus_tag="PKK85_01605"
     CDS             2916..3467
                     /gene="thpR"
                     /locus_tag="PKK85_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNS24749.1"
                     /translation="MRAFMAIEIDFRLINKIIKVQEALKNADAPVKFVDGENLHFTVK
                     FFGDITPDQVQQISSLTNDQLINQKSFQMHIKGTGVFPNLKRPRVLWLGVEDPQEFSQ
                     LQRRLDEEWVRMGFRRERSYIPHLTIGRVKGSRNRDALISKIHELEGVDIGPIAVEKL
                     VLRKSELTPVGPIYTDLQEFPLA"
     gene            3486..4868
                     /gene="cca"
                     /locus_tag="PKK85_01610"
     CDS             3486..4868
                     /gene="cca"
                     /locus_tag="PKK85_01610"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNS24750.1"
                     /translation="MPDLKLILEKIEPSPEEKERVHKLASELIRIINQEAHNQDIPAK
                     AILVGSVAKGTRLAGKADVDIFITFPLSCSEDYLKVKGLELGHHCIEKMGSAYEERYA
                     SHPYVTGFIDGYLVDFVPCYKISSGSELKSAVDRTVLHTKYIKAHLKPVQRGEVLLLK
                     RFMQVVGCYGSEFKVGGFPGYLAELLILNYGTFIRVLEAAAREWMPGYQVDLEGHGTA
                     GFFQDPLVVVDPVDGKRNVAAALTMQKMADFVVAASHYLKNPSQDFFFKKKEEVDLDQ
                     LKKDFLSRGTVVLILTFPSPTIPEDALYPQITKTEQSLVNILKRAGFRLWGSDSACYQ
                     AQVFMILELEVHTLPHLKRHYGPQVWNRVHSESFQKKYQPHCWVEDDTWVALIPREHN
                     TAQSLLKHVISPEGICHLRTGRHLKDQLLENYQLKPLTDFLEEKNLNPQVLIWLHHYL
                     HRNEFSGDIY"
     gene            complement(4912..>5124)
                     /locus_tag="PKK85_01615"
     CDS             complement(4912..>5124)
                     /locus_tag="PKK85_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HNS24751.1"
                     /translation="PIRYKRLTKGLAFTNLGLVDAKVSIDLKRILRLPSSLHSMVSMK
                     CTEVKNLNTFDPFQDAVPQFVYERKD"
BASE COUNT         1427 a         1164 c         1348 g         1185 t
ORIGIN      
        1 aagatggatc tggaacacca gatccatctt ttgacgttga agttcattta cttgtctttc
       61 cagttgttca ttttcctgtt caatcatggc tacgccccct ggaaataata aagactaaat
      121 ctaactatgt ccagactatt atttaaggtt ataagtgtgg caatcaaatt ctattaggag
      181 aagttcttta aagataaaca tggcacctcc cccacttcat tgaagcttac cccaccacct
      241 cccggagtag tataaaaaaa aaccctgatc cggtgttcag atatgatcaa tgttgtatac
      301 gatatcaaaa tttataggga agttctaaga gatattatca aacaggatga tctggtggtg
      361 gagttaggat gccacctggg caattcaacc aaaatcatgg cggaaagagc cccgaagggt
      421 agaataatta caatggacaa atctgaagag gcagcacagg ctatgagtga actgaaaagt
      481 tttcatcata atgtggagtt cttaaaggcg gatgtacgac tgcatgagac actagaaaag
      541 gttgccagga tgctaaagga tcatggtggc tgtgacgtgt tatccattga cctgggtgga
      601 ggccactatc ccgatacaac cttcaaggtg tttttcatct ggtcatcaac tttaagaccc
      661 agagaaacca taattaggaa tagaggactt ttggatttta tgcactcctc atcaagctca
      721 gaaataatta aatcctcgga gggctggttg gagtcctctg gtggggatgg tgttcctccc
      781 cgtcttaaag aactccagct atggtccacc aatatttaat aaggagggtg tttatgatcg
      841 gcaagcgaat caggatcgaa agaatattgg atcggaaaac aggaagatgt gtcattgtac
      901 ccatggatca tggtgtttcc attggaccgg tgccaggcat aatcgacatg gcacacacca
      961 tagacgaagc agccagtggc ggtgcaaatg cagttataat gcataaagga atggttggaa
     1021 gtgggcacag aggatacggg agggatattg gtttgatcat tcacctttca gccagcacag
     1081 gcctgggtcc agatccgaat cataaggtcc tggtgacctc agttgagaag gccatccaga
     1141 tgggagccga tgctgtctcg gttcacgtga acgtgggatc caagagggaa ccagaaatgc
     1201 tcatgcagct gggaagcatt gccgagatct gtgatgactg gggaatgccc ctcattgcca
     1261 tgatgtaccc ccgcggcaag aagatcgaaa acgaacacaa cgttgaagtg gttaaacttg
     1321 catcccgggc tggagcagaa ctgggagccg atatcatcaa aaccaactac acaggagatg
     1381 tggacacctt ccaggaagtc gttgaaggct gccctgtgcc actggtaata gcaggggggc
     1441 cccgggtgga aaccgaccgg gaactcttgg atatggtgaa aaactcggtg gacgtgggag
     1501 gtgctggtgt ggccatcggc cggaacattt tccaggccaa atccccccgg aagaccaccc
     1561 aggccattgc cgagatcgtc cacaacaatc tggatgtgga tgaggcactg gagatactta
     1621 aagggtaagg tagggtggaa agtaaatgaa atttgcctgg ataatggctg agggtacgga
     1681 ctggaaccag aagaagactt tcataaccgc tgctctagag tcagggatgg atcacgtagt
     1741 ggacttcaca gacgtggata gaattcgtaa actaggaaac atcaccatca tatcagatac
     1801 cgaaggatct gacatccaac tggtgggacg caacggagaa ggggacagta ccttagcact
     1861 gccctctgaa ctatccgaat ccaaggacct ggctgccgtg cgtcacttga aaaagagggg
     1921 gaagacaacc gctgcttacg tggagatcac aagtaaaaga cacgaagaac tggcttctct
     1981 tttaggtaga gaggccgact acctgatcct tttaggccgg gactggaaag tgatacccct
     2041 ggagaacatc atcgccgact tgcagaagga gaaagttaag ctcatcgcct cggtggctga
     2101 ctacgaagag gccaaactat ccctggagac cctggaatac ggtactgatg gggttatgct
     2161 ctccccccgt gaagtggggg agatcaagaa ggtggctgat ttactggaga aaattgaaca
     2221 ccaatcatac cccctgaagg tggccacagt taccagggtg gagcctgtgg gcagcggcga
     2281 ccgggtctgc gtggatacct gcagtatgat gtccctgggg gagggtatgc tggtaggatc
     2341 ctacagtcag ggacttttcc tggtgcacag tgaatcactg gagagtgaat acgtggcatc
     2401 ccggccattc agggttaacg ccggtccagt gcacgcctat gtcatggcac cgggtaatcg
     2461 tacccgttac ctatccgaag tccagactgg agacgagatt ctggccgtgg acaaggatgg
     2521 taactctagg aacgtcatcg taggcagggt taaaatagag aaaagacccc tgatgctggt
     2581 ggaagccgaa gccgatggcg aggtactccg gacactactg cagaatgccg agaccattcg
     2641 cctggtggga gaggatggtg accccatatc agtggctgac ctgaaggcag gccataagat
     2701 catggtttac ctggatgaat cagcacgtca ctttggaatg gccatcgaag aaagcatcat
     2761 cgaaaaatag gagttaaatt aacccatctc cttcccctta ttttccatta acaacccttc
     2821 atgaaaatca tcatcatttt aagcatggaa tttagtccac ttctattggg aagttttttt
     2881 aagccagaat gaattgaatg cagggagtga tgagaatgag agcttttatg gccattgaaa
     2941 tagattttag actgataaac aaaattataa aggtgcagga agccctgaaa aatgctgatg
     3001 caccagttaa atttgtggat ggggagaatt tgcacttcac agttaagttc ttcggggaca
     3061 taactcctga tcaggttcag cagataagca gcttgaccaa tgaccaactg ataaaccaga
     3121 aatcattcca gatgcacatc aagggcaccg gggtgttccc taacctgaaa aggccccggg
     3181 tactgtggct aggtgtggaa gatccccagg agttctcaca gttacagcgg agattggatg
     3241 aagaatgggt gaggatgggc ttccgcaggg aacgcagcta cataccccac ctcaccattg
     3301 gacgggtgaa gggttcccgg aacagggatg ccctcatcag caaaattcat gaactggagg
     3361 gggtggatat tggccccata gccgtggaga agttggtgct tagaaagagt gaactaaccc
     3421 cggtgggacc catctacacc gacctacagg agtttcccct ggcttagttg tgaggtgtta
     3481 aatttttgcc tgatcttaaa ctaattcttg agaaaataga gccctcccct gaggaaaaag
     3541 agagggtgca taaactggcc agtgaactca tccgcatcat aaaccaggaa gcccataacc
     3601 aggacatccc agccaaggct atcctggtag gctccgtggc caagggcacc agactggctg
     3661 gtaaagcaga tgtggatatc ttcataacct tccccctatc ctgttcagag gattatctga
     3721 aggttaaagg cctggaactg ggtcatcact gtatagaaaa aatgggaagt gcctacgagg
     3781 aacgctacgc atcccacccc tacgtcacag gattcataga tggttacctg gttgacttcg
     3841 taccctgcta caagatcagc tctggtagtg aacttaaatc tgctgtggac cggaccgtgc
     3901 tccacaccaa atacatcaag gcccatctga agccagtgca gagaggggaa gttctactac
     3961 ttaagcgctt catgcaggtg gtgggttgct atggctccga attcaaggtg ggaggattcc
     4021 cgggttacct ggcggaactg ctcatactga attacggaac cttcatcagg gtgctggaag
     4081 ccgcggccag ggagtggatg cctggttacc aggtggatct ggaaggacat ggcactgcag
     4141 gttttttcca ggaccccctg gtggtggtgg atccggtgga tgggaaacgt aacgtggcgg
     4201 cggccttaac catgcagaag atggctgact ttgtggtggc agccagccat tacctgaaaa
     4261 accccagcca ggactttttt tttaaaaaaa aagaggaagt tgatttagat cagcttaaaa
     4321 aggatttttt gtccagggga accgtggttc tcattttaac ttttccatcc cccacaatac
     4381 ctgaagatgc cctgtacccc cagatcacga agacagaaca gtccctggtg aacatcctta
     4441 aacgggctgg tttccggttg tggggcagtg actcggcctg ctaccaggcc caggtcttca
     4501 tgatcctgga actggaggtg cacaccctcc cccacttaaa gaggcactac ggcccccagg
     4561 tgtggaaccg ggtgcacagt gaatccttcc agaaaaaata ccagccccac tgctgggtgg
     4621 aagacgatac ctgggtggcc ctgataccac gtgaacataa cactgcccag tcactactaa
     4681 aacacgtcat ctccccagaa ggtatctgtc acctgcgtac tggccgacac ctcaaggacc
     4741 agttactgga gaactaccag cttaagcccc ttaccgactt tttagaagaa aaaaacctga
     4801 acccccaggt tttgatatgg ctgcatcatt acctgcatag aaacgagttc tcaggggata
     4861 tctactaaga aggtgttaat tccaaaaaaa gactatccta atttcaactc cttaatcttt
     4921 tctttcataa acaaattggg gcaccgcatc ctggaagggg tcgaaggtgt taagattttt
     4981 tacctcggtg catttcatgc tcaccatgga gtgcagggaa gagggaagtc tcagtatgcg
     5041 tttaaggtct atgcttacct tggcatccac cagccccagg ttggtgaagg ccagtccttt
     5101 tgtcagtctt ttgtaacgga ttgg
//
LOCUS       DAOQVW010000041         5085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_18156_length_5085_cov_2.849105, whole genome shotgun sequence.
ACCESSION   DAOQVW010000041 DAOQVW010000000
VERSION     DAOQVW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18156_length_5085_cov_2.849105"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PKK85_01620"
     CDS             complement(<1..681)
                     /locus_tag="PKK85_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNS24752.1"
                     /translation="MVVATRDNKGKPRILLVHHTAMWYRKPFFKDLSQAYPVQFLFTN
                     VKGYNQIYDTQLSQKIPGMDGVNYQVVPRKWGLAWGAIKRVMGDYDVFLGGSWDTPSD
                     LIETILYFVLVKLKKKPFILWREDWDWKVNSAKRRLVKLFAGFFGRQADAVLVPGSKH
                     QQFFSKLGVSPEKIFTMPNVSNITLKDEDYLHRDRIIRENDLEGKKIVLFVGRLIDLK
                     GVQYLLPAF"
     gene            complement(672..2795)
                     /locus_tag="PKK85_01625"
     CDS             complement(672..2795)
                     /locus_tag="PKK85_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2206 domain-containing protein"
                     /protein_id="HNS24753.1"
                     /translation="MLDLSKLNVKSFLALLLLILLLTDLSITLDLPVLKPVLSLLFFS
                     TIPGLLMFLILKPGPTELAKKIVIWVGLSIFLLMGVGLLLNSLYPFLASPLSLTPLLI
                     AMNLVFIILLVMVYWRNKTNFSGDDLFNFKIDLKDKLLSPLLFPFLLPLLSILGTYLM
                     NTTQNNILLLVMLLLIPLYLIAIGYLNKRVHKATYPLALWLISLSLLLMYGLTSSYLM
                     GRDVHLEYYCYQLSLSNYHWDLNSYYNSYNACISVNILPLIYHVLSGVQGEYIFKAFM
                     ALTGSTIPIIVYLVANKYLSQRYAFFAGILFVFQLFFISLLGAVRQEIAILFFFLTIM
                     VVFDFEMNKSVKKILTIIFIVSTLISHYSTAYVAFVLILPILLLPFFEGLLKDRKLVF
                     TNFDIILISLAFILIWYLVVAKVQFASGAQVIGKTVAATTAGGGGTTSALMATRGAYV
                     LGVLGVVLKSLPNTISILVHDAMFATILVGLYSILRKYHYYKEKFGAQYILGIAISIG
                     LLILFVALPYISIAYDASRLFFQLIIFLAPVFIIGCITIAKWMNKPQWDVVVILILLI
                     SLFTCATYLQYSLLGTPYSAQYENDSIIRQELYIYHKEVKSAQWLYSYGQEDLTIFSD
                     GREVSRFFTAYGENIHNRKINDSFFGWNQSIKEGYIFLGVVNVQRGQVIDIGNDIIRV
                     DLSPYSNLFEKKARVYDNGGSQVWW"
     gene            complement(2920..4047)
                     /locus_tag="PKK85_01630"
     CDS             complement(2920..4047)
                     /locus_tag="PKK85_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS24754.1"
                     /translation="MNILQTPVRFYPYTGGVENYVYYLSRELVKSGNGVEVICANEPP
                     SPEKDSVDGIKVKRLSYTGKVANTNITPYLPLSLSRADCDLIHTHIPTPWSADWSAFY
                     SKVKKKPLVVTYHNDVIGRGLANSVAKIYNSTTLKVVLGQAERIVITQPGYLQSSSHL
                     KHYQDKVEVIPTGVDLEKFKPKAGKEENTIFFLSLLDEFHEYKGLKYLLKALLSVKEV
                     FPSVKLIIGGRGALVGHYQDLVSSWGLEAQVEFAGFIPPEEIAEYFSKANVFALPSIS
                     SLQEGFGIVALEALACQTPVVTTKIVGVASDLEKFKAGLIVPSRDPQKLGEAIIEVLG
                     DQKLQKAMGVRGRKLVEEKYTWQSVALKMEKLYNTIISNKT"
     gene            complement(4091..>5085)
                     /locus_tag="PKK85_01635"
     CDS             complement(4091..>5085)
                     /locus_tag="PKK85_01635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS24755.1"
                     /translation="QDNLVRDGIVFHGVSQPIPLYSGSRRSIKEAIYFALNLIRPLFS
                     EKYDVVDCQGFPFFPCFTAWFHSLIGRSQLVITLHEVWGEYWYQYLGRLGVLGRVVEK
                     LMIHLTSNLIVVSLKTLQDLQKERRIQARIIPNGLDFQKIRGLEPSPQGADVLYAGRL
                     IPEKGVDLIIRTLPLLKKDHPDLKCFIIGDGPERSDLELLASELKLDETVNFTGFLND
                     QEELLKIMKSSQVFVLPSRREGFGMVVLEANACGLPVVVVNHPMNAAVELVKNNQNGF
                     IVDATEKALAEGITAGLKEKKNMQEACIEFARGYDWEEIVLDLEDFYHELASST"
BASE COUNT         1442 a         1123 c         1098 g         1422 t
ORIGIN      
        1 aaatgcaggg agcagatact gtacaccttt aagatcgatg agacgcccca caaataagac
       61 gatctttttt ccctccaaat cgttttcccg gatgatccta tcacgatgaa gataatcttc
      121 atctttcagg gttatgttac tcacattggg cattgtaaaa attttttctg gactcactcc
      181 cagtttagaa aaaaattgtt gatgtttact gccaggcacc agcacggcat cggcctggcg
      241 gccaaagaat cctgcaaata acttcaccaa tcttcgcttg gctgagttga ccttccaatc
      301 ccaatcttct ctccacagga taaagggctt ctttttcaat ttaaccagga caaagtataa
      361 aatagtttca attaggtcac tgggagtatc ccagcttccc cctaggaata catcgtaatc
      421 tcccataact ctttttatgg caccccaggc taacccccat ttcctgggca ccacttggta
      481 attcacacca tccatacccg gtatcttctg ggagagctgg gtgtcgtaga tctggttgta
      541 ccccttcaca ttggtgaata gaaattgaac tggataagct tggcttaaat ccttaaaaaa
      601 tggtttacgg taccacatgg cggtatgatg taccaacagt atcctgggtt ttcctttatt
      661 atccctggtg gctaccacca tacctggctt cctccattat cgtagacacg agctttcttt
      721 tcaaacaagt tggagtaggg tgataaatcc actctgatga tgtcgttacc aatatcgatg
      781 acctggcccc tctggacatt caccaccccc aggaaaatgt aaccctcctt aatggattgg
      841 ttccatccga agaaggagtc attgattttt ctattatgga tattttctcc gtatgcagtg
      901 aaaaatctgg aaacctccct tccatcggaa aatatggtga gatcttcctg gccataactg
      961 tacagccact gggcggattt aacctcttta tggtagatgt agagttcttg cctgatgatg
     1021 ctgtcatttt cgtactgtgc tgaataggga gttccgagca aactgtactg taggtaggtt
     1081 gcacatgtaa acagggaaat taagagaatc aagattacta caacgtccca ttgtggttta
     1141 ttcatccact tggcaatggt tatgcagcct atgataaaaa ctggtgctaa gaatattatt
     1201 aactggaaaa atagccggga tgcatcgtag gcaatggata tgtagggcag ggccacgaat
     1261 aatataagaa gacctatgga gattgctatt cccaggatgt actgggcacc gaatttttcc
     1321 ttgtagtagt ggtatttacg caggatactg taaaggccca ccaggatggt ggcgaacatg
     1381 gcatcatgaa ccaggatact gatggtgttg ggtagagatt taagaaccac ccccagaacc
     1441 cccagaacat aggcaccccg ggtggccatc agtgccgatg ttgttccacc tccccctgca
     1501 gtggtagcag ccacggtttt acctattacc tgggctccgc tggcaaactg gaccttggcc
     1561 accaccaggt accagatcag aataaaggcc aaggagatga ggatgatatc aaagttggtg
     1621 aagaccagtt tccggtcctt cagcaaaccc tcaaagaagg ggagaagcag tatgggcagg
     1681 atgagtacga aggccacgta agccgtggag taatgtgaaa ttagtgtaga gacaatgaaa
     1741 atgatggtta atatcttttt aactgattta ttcatttcaa agtcaaaaac caccataata
     1801 gtcaaaaaga agaatagtat ggctatttcc tgtcgcactg cacctaataa gctgatgaag
     1861 aagagttgaa acacgaagag aattcctgca aagaaggcgt acctctggga gaggtattta
     1921 tttgccacca ggtacacgat gattggaatg gtggacccag taagggccat gaaagccttg
     1981 aagatgtact caccctgcac tccggataga acatggtaga tcaggggtaa aatgttcaca
     2041 cttatacagg cgttgtatga attgtagtag gaattcaaat cccagtggta gttggaaaga
     2101 cttaactggt aacagtagta ctccagatga acgtcacggc ccatgaggta ggaggaagtt
     2161 aaaccataca ttaaaagcaa gctaaggctg attaaccaca atgccagggg ataagtagct
     2221 ttgtgaactc ttttattcag gtaaccaatg gcaatgaggt agaggggtat taacagcagc
     2281 atgaccagaa gcaggatgtt attctgggtg gtgttcatga gataagttcc cagaatagat
     2341 agtaagggta gtaaaaaggg aaataatagt ggggataata gtttatcctt caaatctatc
     2401 ttaaaattaa aaagatcatc gccactaaag ttagttttat tcctccagta aaccatgacg
     2461 agcagaataa taaacacaag attcatggct atgagcaggg gtgttaggga cagtggtgat
     2521 gctaagaagg ggtataaact gtttagtaaa aggcccacac ccatcagtaa gaaaatactt
     2581 aaacccaccc atataacaat ttttttggct aactccgttg gtccaggttt tagtattaaa
     2641 aacatcaaca aacctggaat agtggaaaaa aataaaaggc tcaggacagg ttttagcact
     2701 ggtaaatcca gggtgatgga tagatctgtt aaaagaagta tcaacagtag tagggccagg
     2761 aaacttttaa cattgagttt acttaaatcc aacatcttct tgttcaccta tgaatggcgg
     2821 gttctgattc tttttccata tatatttcaa atcagatatt ctggttatag gactggtaat
     2881 atatacgaag ttttgagggc cttaaaaaat agcttgattt taggttttat ttgatataat
     2941 agtgttataa agtttttcca ttttcagggc cacactctgc caggtatatt tttcctccac
     3001 cagttttctc ccccttactc ccatagcctt ctgtaacttc tgatccccaa gtacttcaat
     3061 aatggcctcc ccaagtttct gcgggtctcg acttggtact atcaaccctg ccttgaattt
     3121 ttctagatca gaagcaactc ccactatttt agtggtgacc actggtgtct ggcaggccag
     3181 ggcctccagt gccacgatcc caaaaccttc ctggagggat gatatggatg gcagtgcaaa
     3241 aacattggct ttactgaaat attcggctat ttcctcaggg ggtatgaacc ccgcaaattc
     3301 aacctgggcc tccaatcccc aggaagaaac cagatcctgg taatggccca ctagagcacc
     3361 tctaccccca atgattaatt tcaccgatgg aaacacttcc ttcacagata ggagggcctt
     3421 caataaatat ttcaagccct tgtactcgtg aaattcgtca agaaggctta ggaagaagat
     3481 tgtgttctct tccttccctg ctttaggttt gaatttctct aaatccaccc cggtgggtat
     3541 cacttccact ttgtcctggt aatgttttaa atgggaagat gattggaggt aacccggttg
     3601 tgtgatcacg attctctcgg cctgccccag cacaactttt agggtggtgg aattgtaaat
     3661 ctttgccact gaatttgcaa ggcctctgcc tatgacatcg ttgtggtagg tgacaactaa
     3721 aggctttttc ttaactttag aatagaaggc actccaatca gcactccagg gggtggggat
     3781 gtgggtgtgg atcagatcac agtctgccct ggatagggat aggggtaaat agggtgttat
     3841 attggtgttt gcaactttac ccgtataaga taatcttttt actttaatcc catctacact
     3901 atctttttca ggtgaagggg gttcattggc acagattact tccactccat tccctgattt
     3961 aaccagttcc cgggaaagat aatacacgta gttttctaca ccaccagtgt agggatagaa
     4021 tcttactgga gtttgcaata tattcatagt ggtcacttaa actagatctt ttttcacact
     4081 tccagataaa tcaggtggag ctggccagtt catggtagaa atcctccaga tcaagcacta
     4141 tttcctccca gtcatagccc cgtgcaaatt caatgcaggc ctcctgcata ttttttttct
     4201 cctttaaacc agcagttata ccctcagcca aggctttttc tgttgcatca acgataaatc
     4261 cgttctgatt attcttaacc aactccactg ccgcattcat gggatggtta accaccacta
     4321 ctggcaatcc acaggcattg gcttccagga ctaccatacc aaacccttcc ctcctgctgg
     4381 gtaggacgaa gacttgtgat gacttcataa ttttcagaag ctcctcctgg tcattcaaaa
     4441 agccggtgaa attaacagtc tcatctaatt ttagttctga agctaataat tcaagatcac
     4501 tacgttctgg gccatctcct attataaaac acttcaagtc gggatgatcc ttcttcaaga
     4561 gtggcagggt tcggatgata agatcaacac ccttctctgg tataagccgc cctgcatata
     4621 ggacatctgc accctggggt gagggttcta aacctcgtat tttttgaaaa tccaggccat
     4681 tgggaattat ccgggcctgg attctcctct ccttctgcag gtcctggagg gtcttcaagg
     4741 acactactat gaggttactg gttaagtgaa tcatcagttt ttcaaccacc ctacccagga
     4801 cacccagtct tcccaggtac tggtaccagt attcccccca cacctcatgg agagttatta
     4861 ctagctggga gcgccctatc agggagtgga accaggctgt gaagcaggga aagaagggga
     4921 aaccttgaca atccaccaca tcatattttt cactgaataa aggtcttata aggttcagtg
     4981 cgaaataaat agcttctttt atggatctgc gactcccact gtagaggggg ataggctggg
     5041 aaactccatg gaacactatt ccatccctca ccaggttatc ctggc
//
LOCUS       DAOQVW010000042         5000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_18600_length_5000_cov_3.797776, whole genome shotgun sequence.
ACCESSION   DAOQVW010000042 DAOQVW010000000
VERSION     DAOQVW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18600_length_5000_cov_3.797776"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..996
                     /locus_tag="PKK85_01640"
     CDS             58..996
                     /locus_tag="PKK85_01640"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS24756.1"
                     /translation="MRIAVTGGKGGTGKSTVATSIAFEMAKVHRTLLVDCDVECPDDH
                     LLLSITREKVKDVETFLPSFDQETCQKCGICSQTCRQNAVVFVTGEYPFLIPEQCNGC
                     GACMLACPWGSITEDSQVIGTIYQGHLEVQESLGLEGVTQGLVNNFLLISGEVAVGCE
                     ASTLVVNATREYASQLEDDYLFTVIDTAAGTHCDVISALMDTNLVLAVSEPTPLGKHD
                     LELILELLEKMGLDAKIIINRADLSSLDLIKTLADEKGVMITLEIPYKKEILENYSRG
                     VPALEGLVSDLVTDLVEEALQDDLVTVEDEMKEEDP"
     gene            993..1883
                     /locus_tag="PKK85_01645"
     CDS             993..1883
                     /locus_tag="PKK85_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS24757.1"
                     /translation="MKTITILSGKGGVGKSTLTSSLAVLLGKENKIVAVDCDVDAPNL
                     GLLLGLKKEDYDSWEYVQVIEKAQLNKAKCKSLKDCVNTCKFGAISWDEDENMPYIND
                     FLCVGCGACVVACPEDAIEFKPVKNAKIGVGETDYGFPIVSGQLMIGESGSGRVVDAV
                     KNRAFQIAEEIQADYVIVDSAAGIGCPVIASVRGSDYVIMVTEPTPVAFSDFKRALEM
                     VKFFAIPHAMVINRWDINPKFTKELENFSKREKIPLIGKIPYDKRFVEALVNLQPAVV
                     YESDFEMIFNKILDFLLTLE"
     gene            2011..3231
                     /locus_tag="PKK85_01650"
     CDS             2011..3231
                     /locus_tag="PKK85_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNS24758.1"
                     /translation="MDSEAQANLKKGEKIAKYSSLVNMFLALLKGMVGVLSGSIALIA
                     DAVNSFSDIFASIAVYIGLRLSQRKPDEKFPYGYYKLETFSSLVVSLIIIFSGVEIAI
                     ESFHSYLNPEPIGIPLISLSVAVISVIMNLVLFRYQGRVGKEIRSQALVNDGKHSLVD
                     ALSSCIVFIGIFLAYIGHTSIQGVAGFIIAILIIYMGLKLGKDALLVLLDVGPDPQQI
                     QTIQSLLKTIPGVEGFHDIKIRRSGPFVFAELHLETKKELPVEKAYELSEKVKMKVKD
                     VITELDTLTVQIEPAKKDKMRFAIPLESNKGWDSSISKHFGKAPYFLIAELKKGKLEN
                     YKIEVNPGKDLEKKRGIKAAEFLAKEAVDVLISGEDVGEGPSYVLMEELIQIVDLEGE
                     DLDEILDNALSMVR"
     gene            complement(3255..4589)
                     /locus_tag="PKK85_01655"
     CDS             complement(3255..4589)
                     /locus_tag="PKK85_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase glutamine
                     C-methyltransferase"
                     /protein_id="HNS24759.1"
                     /translation="MRKDWKITILTPEFYTYGSMVIGGVLGDAGYSVALHKGFQNIPK
                     SDVIFISLQSTIHLLKYRDPILKLPGYKILGGPVTHVPELALELLPVDMVLQGEGEVT
                     VLKIMEELESTFQGNSTPKMKSENDLELVEKNPKIVKGTSSSLIRPIPLIPDDIGSES
                     IRGANVYIETHRGCPGNCGFCQVPCFFGRKVRSRPAADIINEVRTFMEKGTQRIAISG
                     GTGSLYGSPKFNQVDEQAFGSLLKEISNLTSPPNLTIPDIRVDMINDDILDSIRKYTN
                     KWVYFGIESGSEKVLKHMKKGINLDEVRSAVESARKYGLKIAGSFIVAYPGEEEEDFQ
                     DTLDFADELMLDDYFISIAEPLPGTDLKDKIKQIPSDENALFIPGEKYKKYGLSVAEE
                     RALEFMLESYVYRSLPQPMTANLFKALLDEVKAQGKHIRTVTSLLIGCNSYK"
     gene            complement(4670..>5000)
                     /locus_tag="PKK85_01660"
     CDS             complement(4670..>5000)
                     /locus_tag="PKK85_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methanogenesis-associated radical SAM protein"
                     /protein_id="HNS24760.1"
                     /translation="NIGASDLVNVVAADQDIGCLITHQELECLDLADLKETVIIPGRA
                     FVHDQRATEILSQDGIDRMVVRGPDKLTVDGEMSGSLTREDVLKKELIAFEDLIEAIN
                     FFGVRKK"
BASE COUNT         1459 a          981 c         1105 g         1455 t
ORIGIN      
        1 gccggaagag gcggaggtag gggtgcgagg cgacgattct gatgtttagg agggaaaatg
       61 agaatagccg tcactggggg taagggaggg actggaaaat caaccgtggc cacatccata
      121 gcatttgaga tggccaaagt gcaccggacc cttctggtag actgtgatgt ggagtgccct
      181 gatgatcacc ttctactgtc cattacccgg gaaaaggtga aggatgttga aacctttctc
      241 ccatcttttg atcaggaaac ctgccagaag tgcggaattt gttcccaaac ctgccgacag
      301 aatgcagtag tatttgttac aggtgaatat ccatttttga taccggaaca atgcaacgga
      361 tgcggggcat gtatgttggc ctgcccctgg gggtctataa ctgaggatag ccaggttata
      421 ggaaccatct accagggcca ccttgaggtt caagaatccc tgggcctgga aggggtaact
      481 cagggattgg ttaacaattt tttactaata tctggtgagg tggcagtggg ttgtgaggcc
      541 agtaccctgg tggtgaatgc cacccgtgaa tatgcatctc aattagaaga tgattacttg
      601 ttcacagtga tagatactgc tgctggaact cactgcgacg taatatctgc cttaatggat
      661 accaatctgg ttctggctgt ctcggaacca acaccactag gaaagcacga cttagagctt
      721 attctggaac tactggaaaa gatggggctg gatgctaaaa ttatcataaa ccgggcagac
      781 ctgagtagtc tggatttaat taagaccctg gctgatgaga agggtgtgat gataaccctg
      841 gaaatcccat ataaaaagga aatactcgaa aattattcta ggggagtgcc cgcattggaa
      901 ggcttagttt cagacctggt aactgatttg gttgaagaag ccctgcagga tgacctcgta
      961 acagttgaag atgaaatgaa agaggaggat ccatgaaaac catcaccata ctttcaggca
     1021 agggtggggt gggtaagagt accttaacct cctccctggc agtacttctg ggaaaagaaa
     1081 acaaaatcgt tgctgttgat tgtgatgtgg acgcccctaa tctagggtta ctcttaggtt
     1141 taaaaaagga agattatgat tcctgggaat atgtgcaagt tattgagaag gcccaactga
     1201 ataaggctaa atgcaagtcc cttaaagatt gtgtaaatac ctgtaaattt ggagccataa
     1261 gctgggatga agacgaaaac atgccatata tcaatgattt cctatgtgtt ggctgtggag
     1321 catgtgtggt ggcttgtcca gaggatgcta tcgaatttaa accagtaaaa aatgctaaaa
     1381 taggtgtggg agaaactgat tatggatttc ctatagtgtc tggccagttg atgataggtg
     1441 aatcaggttc cgggcgagtg gtggatgccg tcaaaaacag ggcgtttcaa attgcggagg
     1501 aaattcaagc tgattatgta attgtggact ctgcagcagg tataggttgt ccagtaatag
     1561 cctcggttcg gggaagtgac tatgtaataa tggtaacaga gcccacacca gtggcttttt
     1621 cagattttaa aagggccctg gagatggtga agttctttgc aataccccat gcaatggtca
     1681 taaaccgatg ggacattaac ccaaaattca ccaaagaact tgaaaatttt tctaaaaggg
     1741 aaaaaattcc attaattggt aaaataccat atgataaaag gtttgtagaa gctctagtca
     1801 atctccaacc tgcagtagtc tatgaaagtg attttgaaat gattttcaat aaaatacttg
     1861 actttttgtt aacactggag tagtacgggg tgaattaggt ggtactcatc ttaccaagcg
     1921 gggtttattt ttgaatcttt cacagtgtta aaaaatccca aaaaaataaa ttcaacaaca
     1981 atttttttat aaataatctt agggattgat atggattcgg aagctcaagc caatcttaaa
     2041 aaaggagaaa aaattgcaaa atactccagc cttgttaaca tgttcctagc tttattaaag
     2101 ggtatggtgg gtgttttatc tggtagtata gccttaatag ctgatgcagt taactctttt
     2161 tctgacatct tcgcttctat agcagtttat attggcctcc gtctttctca acgtaaaccc
     2221 gatgaaaagt tcccctatgg ttattacaag ctggaaacat tttcatctct tgttgtttcc
     2281 ttaataatca ttttttctgg agttgaaatt gctatcgaat cattccacag ttatctaaat
     2341 cctgaaccaa ttgggattcc tctgataagc ttatcagtag cagttatttc cgtaataatg
     2401 aatttagtat tatttagata tcaggggagg gtggggaagg aaattagatc ccaagcactg
     2461 gtaaacgatg gaaaacacag tctagtagat gcattatctt cctgcatagt ttttataggc
     2521 atatttttag cttatattgg tcatacctct attcagggtg tcgctggatt tataattgcc
     2581 attctgatca tctacatggg gctaaaacta ggaaaagatg cacttcttgt tttgttggac
     2641 gtgggtccgg atcctcaaca aattcaaacc atccaatctt tgttaaaaac aattcccgga
     2701 gtcgaaggtt ttcatgacat taaaatccga agatctggac ctttcgtatt tgctgaactt
     2761 catttggaga ctaaaaaaga gctaccagta gagaaagcat atgaactctc ggaaaaagtg
     2821 aagatgaagg tcaaagatgt tatcactgaa cttgacacac ttacggtaca aatagaacct
     2881 gcaaaaaaag ataagatgcg ctttgcaatc cctttagaaa gtaataaagg atgggattcg
     2941 tccatatcca aacacttcgg aaaagctccc tacttcttaa ttgctgaatt aaaaaaaggg
     3001 aaattggaaa attataaaat tgaggttaac ccaggtaaag atctggaaaa aaagagggga
     3061 attaaagcag cggaatttct ggccaaggaa gctgtggatg tactaatctc tggtgaagat
     3121 gtgggtgaag gacccagtta cgtgttaatg gaggagttaa ttcaaatagt tgatcttgaa
     3181 ggagaagatt tagacgaaat tttagacaac gccctaagta tggtccgatg actagggtgt
     3241 ggtggcttca gaaattattt gtaggaatta cagcctatga gtagggacgt cacagtcctt
     3301 atatgttttc cctgtgcctt cacttcatct aaaagagctt tgaataaatt cgcagtcatt
     3361 ggttgaggta aactacgata cacgtaggat tccagcatga attccagggc cctttcttca
     3421 gccacgctta aaccatactt tttgtacttc tcacctggga tgaaaagtgc attttcatct
     3481 gatgggattt gttttatctt atcttttagg tcagtgccag gcaatggctc tgcgatactt
     3541 atgaagtagt catccagcat taattcatca gcaaagtcca gggtgtcctg gaaatcttct
     3601 tcttcttcac cagggtaggc cacgataaaa gaacccgcta ttttaaggcc atatttccgg
     3661 gcagattcca ccgcactccg gacttcatcc aaattaatac ccttcttcat atgttttaaa
     3721 accttctcac tcccggattc tatgccaaaa taaacccact tattggtata ctttctgatg
     3781 gaatccaaga tatcatcatt aatcatatcc acccttatat ccgggatggt aaggttaggt
     3841 gggctggtta aattcgatat ttccttgagt aggctgccga aggcctgttc atccacttgg
     3901 ttgaatttgg ggctgccgta taaggaaccg gtacccccac ttatggcaat tcgttgggtt
     3961 cccttttcca taaatgtcct gacttcatta attatgtctg cagcaggtcg acttctaact
     4021 tttcttccaa aaaaacaggg gacctggcaa aaaccacagt tacccggaca tcctctatga
     4081 gtttcgatgt aaacgttggc cccccttata ctctctgaac cgatatcatc tggtatgaga
     4141 ggaattggcc taattaacga tgatgatgta cctttgacaa ttttaggatt cttttccacc
     4201 agttctaggt cattttcaga tttcatcttg ggggtggaat ttccctggaa tgttgattcc
     4261 agttcttcca ttatttttag gacggtaact tctccctctc cctggagtac catatctact
     4321 gggagcagtt ccaaggccaa ctcagggaca tgggtaacgg ggcctcccag aatcttataa
     4381 cccggcaatt ttaggatggg atcccggtat tttaataggt gaatagtgga ctgaagacta
     4441 ataaaaataa catcagactt gggaatgttc tgaaagcctt tatggagggc cacagagtat
     4501 ccagcatccc ccaggacacc cccaattacc atactaccat atgtgtagaa ttcaggggtt
     4561 aaaatcgtta ttttccaatc tttcctcatc tcaaacccta caacatctat tgatgtgatg
     4621 ctaaaataaa tccattcact acgtcaactt gaatcaggtg atctaatttc tattttttcc
     4681 gaactccaaa gaagttaata gcttcaatta agtcttcaaa tgctattaat tcttttttca
     4741 acacatcttc tctggtgagg ctgccactca tttcaccatc aacggtgagt ttatccgggc
     4801 ctcgaaccac cattctatct atcccgtcct gactcaatat ctcggttgcc ctttgatcat
     4861 ggacaaaggc tcttccaggg attatcacag tctctttcaa gtcagccagg tccaagcatt
     4921 ccagttcttg atgggtgatc aagcagccta tgtcctggtc ggcagcaact acgttgacca
     4981 gatcagaggc cccaatattt
//
LOCUS       DAOQVW010000043         4962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_18806_length_4962_cov_3.081516, whole genome shotgun sequence.
ACCESSION   DAOQVW010000043 DAOQVW010000000
VERSION     DAOQVW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18806_length_4962_cov_3.081516"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKK85_01665"
     CDS             complement(<1..429)
                     /locus_tag="PKK85_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNS24761.1"
                     /translation="MKFEVGKLLYQGKAKDVYETDHPDQVMVKFRDDITAGDGQKKDT
                     LGEKGRYNSIISAKLFQLLSSEGVNTQYLDLPEPSYMLCKKLDMIPLEVITRNIATGS
                     LVRRFPFYEGQELDPPLIQMDFKNDEYHDPMLNDDIAIALG"
     gene            566..2344
                     /gene="glmS"
                     /locus_tag="PKK85_01670"
     CDS             566..2344
                     /gene="glmS"
                     /locus_tag="PKK85_01670"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNS24762.1"
                     /translation="MCGIVGCILKDKKAAPILLDCLRRLEYRGYDSVGIATSSSSINI
                     RKDSGKIDEVHERLQLDELPGNVGIAHVRWATHGLPTRENAHPHTDCENKIVLAHNGI
                     IENYHELKDQLQEEGHHFLSDTDTEVIVHLIEKEMKGGMDFEEAVRETTQKIKGSYAL
                     AIIHADYPDTIIGVQKESPLILGVGDGDYFLASDVPAILEETNRIIYLDDDDIFIIRS
                     DGITIKDANGRVKENKVDVINWSPEMAQKGGYDHFMIKEIHEQPEAVRNTLSELGEIK
                     KTVKNFSKFKRICFVACGTSYHASMVGKYLFEELMGLPVDLALASEFEFSVGTLRPDT
                     LVIFITQSGETADTMKAMELAKEKAETLAIVNVIGSRATREADHVIYTRAGPEIAVAA
                     TKTYVSQLTCIYLLAACMAGDDELIDELRKVPDHMLAALDQEDFIKEISPHYRDAQDF
                     FFIGRGFSYPTAMEGALKLKEITYIHGEGYAAGELKHGPLALISEGVPVVAVVPPGKK
                     HDRTMGNVKEVKARGARVIGLGSLEDTNLRSESHDMIGLDDTISEHISPLVYVVPLQL
                     LAYYISVERGINPDQPKNLAKCVTVV"
     gene            2550..2813
                     /locus_tag="PKK85_01675"
     CDS             2550..2813
                     /locus_tag="PKK85_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24763.1"
                     /translation="MKPQKYGKLFIVLMIALLAYGFASGVHALGIGENVGKNLLASDW
                     SLSGDQKISEVSDPSFKPAKLNRRVVNVTNTTIPKNITTNETQ"
     gene            2894..3469
                     /locus_tag="PKK85_01680"
     CDS             2894..3469
                     /locus_tag="PKK85_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNS24764.1"
                     /translation="MQERNTIGPKIRQLRKNRDMTVEQLAESSQCNAELIKNLEEGAL
                     VPSLTPLLRIAKVLGVRLGTFMDDHPQNGPFKLEADKHENLIRFSGKDATTDQSTLDF
                     YSLAYGKGDRHMEPFIIDVHHMPADEYKLSSHEGEEFIYVLKGSIEIFYGQQKFLLNE
                     GDSIYYDSIVPHDLHAHQEDAKILAVVYAPL"
     gene            3490..3900
                     /locus_tag="PKK85_01685"
     CDS             3490..3900
                     /locus_tag="PKK85_01685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNS24765.1"
                     /translation="MLNIVVTPRFGDTDGLRHINNIALAEWFELARNPLYRLFTPDLD
                     LRYEKWKLIMVRTEFDFLGQMYYGSDVHIKTYISKIGNTSFITKHEAWQNGHLRAQGK
                     AVIVHFDFVEQKSKSIPDHIRKMLMKHLEDEEKE"
     gene            3914..>4962
                     /locus_tag="PKK85_01690"
     CDS             3914..>4962
                     /locus_tag="PKK85_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS24766.1"
                     /translation="MLFTKNTLGYFLEKGAQEHPDRDFMVYPDRDLRLSYKEFDERVN
                     LLAKGLLSIGIKKGDHVGIWALNVPDWLTFMFATAKIGVVLVTVNTAYRSHELAYVME
                     QSDMKALAIIDGFRDIDYVQTLYELIPELKTQERGELKSEKFPYLKSVIYIGPEKHRG
                     MYNTPELLLLGKHTPDDEFLQIKASLDGDEVINMQYTSGTTGFPKGVMLSHINILNNG
                     YYIGERQKFTGEDKLCITVPLFHCFGIVLGIMATLTHGSTNVMVEVFDPLLVLAAVQK
                     EKCTALYGVPTMFIAEYSHPMFDMFDLSSLRTGIMAGSTPPIEAMKKVVNNMHMAEVT
                     SVYGLTEGSPGFTQT"
BASE COUNT         1528 a          966 c         1109 g         1359 t
ORIGIN      
        1 gcctaatgca atggctatat catcgtttag catggggtcg tgatattcat cgtttttaaa
       61 atccatctga ataagggggg gatccaattc ctgcccctca tagaatggaa aacggcgcac
      121 cagacttcct gtagcaatgt tgcgtgttat gacctccagt ggtatcatat ccagtttttt
      181 acagagcatg taacttggtt ccggaagatc caggtactgg gtattcacac cttcagatga
      241 tagtaactgg aataacttag cagatataat ggaattgtag cgacctttct caccgagtgt
      301 atcttttttt tggccatcac cggcggtaat atcatccctg aatttaacca taacctgatc
      361 tggatggtca gtttcgtaaa catccttggc tttgccctgg tataagagct ttcccacttc
      421 aaacttcatg tttaactcca ttttcatttg tatccaaggt ttaatatacc ctttaaacta
      481 caattatttt atttgtgata aggtaagttt aaaattatat atagtaagac ctagtttaac
      541 taaaaaccat tttttggtga aatacatgtg tggaattgta ggatgtatac ttaaagataa
      601 aaaggcagca ccaatactcc tagattgcct acgacgtttg gagtatcggg gttatgattc
      661 ggtgggcata gccacctcat catcatcaat taatattaga aaagacagtg gaaaaattga
      721 tgaagtccat gaaagattgc agttagatga actaccaggt aacgttggaa ttgctcacgt
      781 ccgctgggca actcatggac tacccactcg tgaaaatgcc catccacata ctgattgtga
      841 aaataaaatt gttctggcac acaacggcat tatagagaat tatcatgaat taaaagacca
      901 gttgcaggaa gaaggtcacc atttcctttc agatactgat accgaggtga tcgtccacct
      961 gatcgaaaaa gaaatgaaag ggggaatgga ttttgaggaa gcggtgcgtg aaacaaccca
     1021 gaagattaag ggctcctatg ccttggcaat cattcacgct gattatccag acacaatcat
     1081 cggggtacaa aaggaaagtc ccctcatttt aggggtgggg gatggtgatt atttcctggc
     1141 ctcagatgta ccagccatac tggaagaaac taatcggatc atctacctgg atgatgatga
     1201 tatattcatt atcagaagtg atggcatcac cattaaggat gcaaatggta gggtaaaaga
     1261 gaataaggtc gatgttatta attggagccc tgaaatggcc cagaagggtg gctacgatca
     1321 tttcatgatt aaagaaattc atgaacaacc ggaagcagtc cgtaacactc tatctgaact
     1381 tggagagata aaaaagactg tgaaaaactt ttctaaattt aaaagaatat gttttgtggc
     1441 atgtggaact tcatatcatg catcaatggt tggaaaatat ttattcgaag aattaatggg
     1501 tcttccagtg gatctggcat tggcatctga gtttgaattt tctgttggta ctttaagacc
     1561 ggatactttg gttatattca tcacccaatc tggtgaaact gcagacacca tgaaagccat
     1621 ggaattggcc aaagaaaagg cagaaaccct ggcaattgtg aatgtcatag ggagtagagc
     1681 tacccgtgag gctgatcatg tcatttacac tcgagctggt ccagaaattg ctgtagctgc
     1741 cactaaaaca tatgtaagcc agctgacttg catatatctc ctggcagcat gtatggctgg
     1801 agatgatgaa cttatagatg aactcaggaa ggtgccggat catatgctgg cagcactaga
     1861 tcaggaagat tttattaaag aaatttcacc tcactaccga gatgcccaag atttcttctt
     1921 cattggtaga ggattttcct atcccactgc catggagggc gcccttaaat tgaaggagat
     1981 cacctacatt catggagaag gatacgctgc cggtgaactg aaacacgggc ccttagcctt
     2041 gataagtgaa ggagtaccag tggtggcagt ggtgcctccc ggcaaaaaac atgacaggac
     2101 catgggtaat gtaaaagagg ttaaagcacg tggagcacga gtgattggtt taggttccct
     2161 ggaagacacc aatttgcgct ctgaatccca tgatatgata gggctggacg ataccatcag
     2221 cgaacatata tcacccctgg tttatgtggt gcccttacaa ttactggcat actacataag
     2281 tgtggaaaga ggaataaacc ctgatcaacc aaagaatcta gctaaatgtg ttacggtagt
     2341 gtgatacgtc tttcctgata ttgtgaagct tctaatgaag gatgtaacta cttttataaa
     2401 ttttcatcct ctttgggagc atggagttta gaatgctatg gatagcatat ttaaagatga
     2461 aggaaaatat agtttcttgt gaaattgatg accacttggt gataaataat tttcatatct
     2521 atggagttag gtcattctga ggttgctaga tgaaacccca aaaatatgga aaactgttca
     2581 tcgttctcat gattgcactt ttagcctacg gatttgcatc aggagtgcat gcactgggaa
     2641 taggagaaaa tgtggggaaa aatcttcttg cttctgattg gagcctttct ggggatcaga
     2701 agattagtga agtcagtgac ccttctttta aaccagccaa attgaacaga cgggtagtta
     2761 acgttaccaa caccaccatt cccaaaaaca taactaccaa cgagacccag taagttcaaa
     2821 aagcgagatt gattccgact attctaaaaa ttctagtaag atggcctagt atgagtttta
     2881 ttcaggtgat ttgatgcagg aaaggaatac aattggacca aaaatccgac aattaagaaa
     2941 aaatcgtgac atgaccgtgg aacaactggc tgaaagcagc cagtgcaatg cggaactgat
     3001 aaaaaacctg gaggaggggg cactggtccc atctctcact ccacttttac ggattgccaa
     3061 ggttctagga gtgcgtctag gaacatttat ggatgatcac cctcaaaacg gcccctttaa
     3121 attggaagct gataaacacg agaatctgat aaggttctct ggtaaagacg ccaccacgga
     3181 tcaaagcacc cttgatttct attcacttgc atatggtaaa ggtgataggc atatggagcc
     3241 tttcataatc gatgttcatc acatgccggc tgacgaatat aaactttcct cccacgaggg
     3301 tgaagagttt atttacgtgc tcaaaggtag tatcgagatt ttttatgggc aacagaaatt
     3361 cctactcaac gagggtgata gcatatatta tgattctata gtcccccatg atttacacgc
     3421 tcatcaagaa gatgctaaga tattagcagt ggtttatgca ccattatgag ttaccattaa
     3481 gtgacttaca tgttaaatat tgtagtaact ccccgtttcg gagataccga tggactcaga
     3541 cacatcaata acatagccct tgcggaatgg tttgaactgg ccagaaatcc actttatcgt
     3601 cttttcaccc cagacttgga tcttcgttac gaaaaatgga agcttatcat ggttcgcacc
     3661 gagttcgatt ttttgggaca gatgtactac ggtagcgacg ttcatataaa aacgtacata
     3721 agcaaaatcg gaaacacttc tttcattact aaacatgagg cctggcaaaa tggccattta
     3781 agggcccagg gaaaggcagt tatagttcat tttgattttg tggagcaaaa aagtaagtcc
     3841 attccagatc acatcagaaa aatgctgatg aagcatctgg aggatgaaga aaaagaatag
     3901 aaaaggtgtc atcatgcttt tcaccaaaaa tactttagga tatttcctgg aaaaaggggc
     3961 tcaagaacac cctgatagag attttatggt ctatccggac cgtgacttga gattgagtta
     4021 taaagaattt gatgagcggg taaacctact ggccaaagga ttattgtcta tcgggattaa
     4081 aaagggcgat catgtgggta tatgggccct taacgtaccg gattggctta cctttatgtt
     4141 tgccacagcc aagattggtg tggttctggt aacagttaac accgcctacc gcagtcatga
     4201 actggcctac gtgatggaac agtctgatat gaaagcattg gccataatag atggattcag
     4261 ggacatagat tatgttcaaa ccctttatga actgattccc gagctgaaaa cccaggagag
     4321 aggagaatta aagagtgaaa aatttcctta tctaaagagt gttatttata tagggcctga
     4381 gaagcaccgg ggaatgtaca acacgcctga gttactgtta ctgggaaaac acacccccga
     4441 cgatgaattc ctacaaataa aggcgagttt agatggtgat gaagttataa acatgcaata
     4501 cacatctgga actactggat tccccaaggg agtaatgtta tcccatatta atatccttaa
     4561 caatggttat tacattggtg aacgtcaaaa attcactgga gaagataagt tatgcatcac
     4621 cgttccccta ttccattgct tcggtattgt gctgggaatc atggctactt tgacacatgg
     4681 atccaccaac gtcatggtgg aagtttttga ccccctgctg gtcttggctg cagtgcaaaa
     4741 agaaaaatgc acagctctct acggtgtccc taccatgttt atagctgagt acagtcaccc
     4801 catgtttgat atgttcgacc tttccagcct gcgcacgggt atcatggcgg gttccacccc
     4861 acccatcgag gccatgaaaa aggtggtcaa caacatgcac atggctgagg tgaccagtgt
     4921 atacggactc acggaaggtt caccggggtt cacccagacc ag
//
LOCUS       DAOQVW010000044         4924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_18995_length_4924_cov_3.972068, whole genome shotgun sequence.
ACCESSION   DAOQVW010000044 DAOQVW010000000
VERSION     DAOQVW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18995_length_4924_cov_3.972068"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..721
                     /locus_tag="PKK85_01695"
     CDS             389..721
                     /locus_tag="PKK85_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24767.1"
                     /translation="MVYDFLLPSLSVLVGYLFTYTLYKKKLLKKTLHVNLWNFIIGIV
                     FLVSGGGGFLLLILMELGIILPLSPQLLYWHVELGISLVLVTLFHFHCYWRSARKMIF
                     LKNRRQEH"
     gene            747..1427
                     /locus_tag="PKK85_01700"
     CDS             747..1427
                     /locus_tag="PKK85_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24768.1"
                     /translation="MPRKKKVIGSLASIPLVAAATMSGSCAGGCPYGMVNDPYPGQCR
                     LYTDSSGDGICDLSQVLTSSDTASSASNTDSTTSDNVNYDQANPGNSPETDNASATLE
                     TGIYGSDGASLGVDDYNYHVLPLSFLLIASYMFTYLLFQKGILKPGIHKRIWNLLVTV
                     GYAGVGVTGVLLTLMVNMGIKAYSQGITFWHAETAILMVIGTLIHLHIYRKPFKRMFK
                     VLFRWESH"
     gene            1676..1951
                     /locus_tag="PKK85_01705"
     CDS             1676..1951
                     /locus_tag="PKK85_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNS24769.1"
                     /translation="MKKLLWWIIAGTKGGINRARIIESLHQRPYNAHQLAEKLGLDYK
                     TVRHHIKVLEQNRVISSSGEKYGMMYFLSPQMEESYEIFEEIRGQMN"
     gene            1969..2331
                     /locus_tag="PKK85_01710"
     CDS             1969..2331
                     /locus_tag="PKK85_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24770.1"
                     /translation="MDKVEVIGNGTGHGPRGELNNVLSFNDPGMATLAAFVGLINISL
                     LLVMFGIYLSSYRRLKSRFTLGLLFFTLLLLLQNALFMLFLIIGEGFRGPGMGYPVLS
                     LNIIQLGALIILLRITWD"
     gene            complement(2354..3346)
                     /locus_tag="PKK85_01715"
     CDS             complement(2354..3346)
                     /locus_tag="PKK85_01715"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNS24771.1"
                     /translation="MTKKIVLGLPKGSLNNVNRGNTYQLFVDAGYEVKGYEPGKEDNE
                     IQILNDPEIRGFLSRPQSAPVELNRGILDLAIVGEDWIREESANLEENLTTKLGDLDY
                     GQTRLIVAVPNEAPYGSLTEFFHANQERKRPILCFTEYPNLTKQYFMKNEGYQELYGN
                     KTPLVQVRGLRDGENDRVQIINSDGATEVYIAKGADLVVDNTQTGSSLRKAGLKILDT
                     IMESSAGLYAGPSCTGPKLQKAQMIYEQLYGATKARKYFDVKFNVSNKKIGVVKEFLL
                     SQGFCSDEPTVVQGSSFSQVNVLIPKNMFPEMLQAVKGYGASAIVRENVKQYVE"
     gene            complement(3416..4198)
                     /locus_tag="PKK85_01720"
     CDS             complement(3416..4198)
                     /locus_tag="PKK85_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNS24772.1"
                     /translation="MNLSHWMSCYQKILECFGYSQKQDEKSAQLLNQLLTLQGGLSPD
                     DIPVKEKAIIFGAGPSLKWNIHQLQEQDKLHQIKLDEFTIIAADGATTALLEEGMVPE
                     VVVTDLDGELENLYQAQRQGTVLVVHAHGDNQEKLKKHVPHMDRVLGTTQSWPRGMVY
                     NWGGFTDGDRAVFIAAKLGVKIIILAGMDFGTHTTRYSRPELSFEVAEADEIKQLKLH
                     YAKKLVEWAAKHENVLILNISGGETIKGVENIPASRIKECLS"
     gene            4271..>4924
                     /locus_tag="PKK85_01725"
     CDS             4271..>4924
                     /locus_tag="PKK85_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNS24773.1"
                     /translation="MDETLLMIPGPTRVAPRVLKAMSENIVNHRSAIFGQILKDTSQM
                     MSDVFRTTNKSYLLTGSGTAAMEAAMVNILEKGDKVLNVVGGKFGQRFMQIVEAHGGT
                     SMPLEVEWGQAVTPDEVGFLLEEHDDIKAVTVVHNETSTGVVNPIKELGKILQNHDAL
                     YVVDTVSSLAGDDVEVDDYGIDICVTGSQKCMAAPPGMAAITLSQDAWDVVNRPNSAS
                     "
BASE COUNT         1220 a         1133 c         1147 g         1424 t
ORIGIN      
        1 ccccacgtag agcaggtcgg aggcacatgg ccagggcttc catttgcaga tccctatgta
       61 aaaggcggtc tggaatgtaa tcgggattga atgcctccac atttctaaaa agagtttctt
      121 ctcccattag tatgtcttta attgtcattt taaagatacc cgttttttta actcatataa
      181 gaatttataa cattattatg gtctagcccc taatataacc ctacattcca catggtatcc
      241 tgaaccctgc tttatggtta tcatccatga aatgggggtt aattgggcta gatttgggta
      301 taaacttgga aaaaatactt ttatactggt cttcttcata tttcttgtca cttaatattt
      361 tttaatttat taaaaaggag gaagagtaat ggtttatgac tttctattac cctccctatc
      421 agtgttagtg ggttatttat tcacttacac cctctacaag aagaaattat taaaaaaaac
      481 attgcatgtg aatctctgga atttcataat aggcattgtc ttcctggttt caggtggggg
      541 aggatttcta ctcctgatcc tgatggaact gggaatcatt ctccccctca gcccccagct
      601 actttactgg catgtggagc tgggaatcag tctggtgcta gttaccctgt tccatttcca
      661 ctgctactgg agatccgcac gtaagatgat atttttaaaa aatcggaggc aagaacatta
      721 aagaaaaact taaagatgtt ctggctatgc cccggaagaa aaaggttatc gggtcgctgg
      781 cctctatccc cctggttgct gcggccacta tgtcgggttc ctgcgctggg ggttgcccct
      841 acggaatggt taacgacccc taccctggcc agtgccgact ctacaccgac tccagtggtg
      901 atggcatatg tgacctttcc caggtgttaa cttccagcga caccgcatcc agcgcttcca
      961 acaccgattc tactacctca gataatgtta actatgacca agcaaatccg gggaactctc
     1021 ctgagactga taatgccagt gccaccctag aaaccggtat ttacgggtca gatggtgctt
     1081 ccctgggggt ggatgattac aattaccatg tgctgcccct gagtttcctg ttgattgcca
     1141 gttacatgtt cacctacctc ctcttccaga agggtatcct aaagcccgga atccataaaa
     1201 ggatctggaa cctgctggtg acggtaggtt atgccggagt aggagtgacg ggggtcctgc
     1261 tgaccctgat ggttaatatg ggaataaagg cttacagtca gggcattact ttctggcacg
     1321 cggagactgc aattttaatg gtaatcggta ccttgataca tctccatatc taccgaaagc
     1381 cattcaaaag gatgtttaag gttcttttca ggtgggaatc ccattgaact aagccatcta
     1441 gaattccttg ttcaaagaag aagcagctga attttaccaa caatcaaaat gagagtactt
     1501 gatcatataa gtttaggatt aattgaaaag gggcgtggtg gcactggtat actgtcctta
     1561 gtaatatttt ttcagaaata gtggaattag tctataccat aaatttattt atctaaaaca
     1621 catattacca gaagattagg ctgaaggtag cttgaaattt agttttgtga tattcatgaa
     1681 aaagttactc tggtggatca tagccggtac caaaggcggt atcaatcggg ccagaattat
     1741 agaatccctc caccagagac cctataatgc acatcagctg gcagaaaaac tgggtttgga
     1801 ctacaagact gtgcgtcatc atattaaggt cttggaacag aaccgggtaa tttcatcttc
     1861 tggtgaaaag tatggaatga tgtactttct atccccgcag atggaggaaa gttacgagat
     1921 ttttgaagag ataaggggcc aaatgaatta gtaattaggt gatggggaat ggacaaagta
     1981 gaagttattg gaaatgggac tggacatggt ccaagaggag aattaaacaa tgttttatca
     2041 tttaatgatc cgggtatggc caccctggcg gcttttgtgg gattgataaa catctctctg
     2101 ctcctggtga tgttcgggat ctacctatcc agttaccgcc gactcaaatc cagattcact
     2161 ctaggcctgc tattcttcac cttactctta ctccttcaaa acgcgctttt catgctcttt
     2221 ttgataatag gggaaggttt ccgtgggcct ggaatggggt accctgttct tagtcttaac
     2281 atcatccagt taggggccct tattattttg cttagaatta cttgggacta gaaaaaaacc
     2341 cggtaagtgg tagctattcc acgtactgct tcacattctc ccgtacgata gccgaagccc
     2401 cgtagccctt aactgcctgt aacatttccg ggaacatgtt tttaggtatc aagacattta
     2461 cctgggagaa gctgctgccc tgcaccacgg tgggttcatc agaacagaac ccctgggaca
     2521 gtaaaaattc cttaactacc cctatttttt tattggaaac gttgaactta acatcaaagt
     2581 acttccgggc cttggtggca ccatataact gttcatatat catctgggcc ttctgtagtt
     2641 tgggtccggt gcagctggga ccagcgtaca atccagcgct ggattccata atggtgtcca
     2701 ggatcttcaa acccgccttg cggaggctgc tgccggtctg ggtgttgtcc acaaccagat
     2761 ctgccccctt agctatgtaa acctcggtgg ctccgtcgga gttgatgatc tgcacccgat
     2821 cattctcacc atccctcaag ccccgcacct ggaccagggg cgttttattc ccatatagtt
     2881 cctggtaacc ctcatttttc atgaaatact gtttggtgag gttaggatac tcggtgaaac
     2941 acagtatggg tctttttcgt tcctggttgg catggaagaa ttcggtgagg gacccatagg
     3001 gtgcttcatt gggcaccgcc actatgagcc gggtctgtcc atagtccaga tcacccagtt
     3061 tagtggtgag attttcttca aggttggcag attcttccct tatccagtcc tcgcccacga
     3121 tggctaggtc taggattcca cggttaagtt ccaccggtgc actctggggt cgggaaagga
     3181 atcctctaat ctcggggtca ttcaggatct gtatctcatt atcttcctta ccaggttcgt
     3241 agcccttgac ctcgtagccg gcatccacga ataattgata agtgtttcct cggttaacgt
     3301 tgttcagact cccctttggg agtcccagca ctattttctt ggtcatgatc ttacaccttc
     3361 aaaattatct ttatataatc attcacagtt aaaaaataca ccgcttcgaa agaattcagg
     3421 ataaacactc ctttatcctg gaggcaggga tgttctcaac tccttttatg gtttctccac
     3481 ctgagatgtt caggataagc acgttttcat gtttggctgc ccattccacc agtttttttg
     3541 catagtgaag cttaagttgc tttatttcat ctgcttcagc cacttcaaaa gacagttcag
     3601 gtcgggagta gcgggttgtg tgggttccaa agtccatgcc tgccaggatt atgatcttca
     3661 ctcccagttt tgcggctata aagactgccc ggtccccgtc ggtgaaaccc ccccagttat
     3721 aaaccattcc ccttggccag gactgggtgg tgcccaggac tctatccatg tgaggtacat
     3781 gttttttcag tttttcttgg ttatctccat gggcatggac caccagcacc gtcccctgcc
     3841 tctgggcttg gtaaaggttc tccagttccc cgtccaggtc agtcaccacc acctccggga
     3901 ccatgccctc ttccagaagg gcggtggtgg ctccatcagc ggctattatg gtgaattcat
     3961 ctagttttat ctggtgtagt ttgtcttgct cctggagttg gtgtatgttc catttgaggg
     4021 atggtcccgc tccaaaaata atggcttttt ccttcactgg gatatcgtcg ggagataagc
     4081 caccctgaag agttaatagc tgatttagta gttgtgcaga tttttcgtcc tgtttttggc
     4141 tgtatccaaa gcattctaga attttttggt agcaggacat ccaatgggag aggttcatat
     4201 gaagagatta tatgtcctag gaatataaat gaaattactg atgataatct agaacttgga
     4261 ggatttcaag atggatgaaa ctttgctaat gataccagga cccacccgag tagctccccg
     4321 ggtgcttaaa gccatgtccg agaacattgt caaccaccgg agtgccatat ttggccagat
     4381 attaaaggac acctcccaga tgatgtccga tgttttccga accaccaaca agtcctacct
     4441 cctaaccggg tcaggtaccg ctgccatgga ggctgccatg gtgaacatcc tggagaaggg
     4501 ggataaggtc ctgaacgtgg tgggtggtaa gtttggccag cgcttcatgc agattgttga
     4561 ggcccatgga ggaacttcca tgcccctaga ggtggagtgg ggtcaagccg ttacccctga
     4621 tgaagtggga ttcctcctgg aagagcatga tgatattaaa gccgttaccg tggtgcacaa
     4681 tgagacttcc acaggggtgg tgaaccccat taaagagttg ggcaagatcc tccagaacca
     4741 tgatgccctc tacgtggtgg acactgtgtc ctccctggca ggtgatgatg tggaggtgga
     4801 tgactatggc atcgatatct gtgtcaccgg ctcccagaag tgtatggctg ctccgcctgg
     4861 tatggccgcc ataaccctct cccaggatgc ctgggatgtg gttaaccgtc caaactccgc
     4921 cagt
//
LOCUS       DAOQVW010000045         4924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_18998_length_4924_cov_3.211337, whole genome shotgun sequence.
ACCESSION   DAOQVW010000045 DAOQVW010000000
VERSION     DAOQVW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18998_length_4924_cov_3.211337"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKK85_01730"
     CDS             <1..399
                     /locus_tag="PKK85_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24774.1"
                     /translation="DQGDWDFIADNLNHIRETEAIPGLVTHRPFHTTSKLLKSPVKDL
                     FELYMIPVNRLGYLMDCEQNLEEERKLLREMVLKLDKTIIGKKLLAAGIMTPHDAFEY
                     LKLLDYVDIVAVGIASAKEAHETFGVLSKK"
     gene            complement(432..1373)
                     /locus_tag="PKK85_01735"
     CDS             complement(432..1373)
                     /locus_tag="PKK85_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNS24775.1"
                     /translation="MHREELLLDITKTLTNHGFETSNIYDRSCFDLVARRELILLLMK
                     VLVNVDGFSMEQAREIKMVANTLLASPLVVGAKSKNEYLEEDVVYERHGIPVIGMETF
                     RNLIIDNLYPEVFAGRGGYYVQIDGQIIKELREKQDLSLKELADLVHVSRETIYKYET
                     GRVRAHPETAMILERILNMKITVSVNLFQVPVTTQDAKIKESKHRELEELGFGVIKTN
                     RTPFDALARPNQSKSKDAQPIIANLDKQRNQKLLAKMAMNLRDISSITDTEAAFILAN
                     RQNLKSIEGVPVVHNWEMEELKSSQDFMKLLKERRDS"
     gene            1415..2683
                     /locus_tag="PKK85_01740"
     CDS             1415..2683
                     /locus_tag="PKK85_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HNS24776.1"
                     /translation="MYNNILHVGFDDTDSSSGMCTTYVCAVVLERLKACGMTIRGEAR
                     LIRLNPFAPYKTRGNGAVSFSVVLDGAGVVERVKKIVLATVEEYSQMDDPSTNPGVVF
                     YHGPITEDLQEYSLRTVQSMVTLEEAEKLAQKIEAEVFKYKNGRGVIGALAAIGCPLI
                     DKTYELIAYREVHNYGKVRRVNEDSVRLMDRATYPSTFDNLYEDHITITPHTPCPVLY
                     GIRGESKEAVYRAHNLVKSEEPIERFMVFLSNQHTDMHLQNTDGIAGMEQYQCYRVAG
                     EVNSTPYVIEGGHVIFNLKDKSGEIECAAYEPTKGFRDTVRGLAPGDDLIVYGGIGDK
                     GTLNVEKIQILHLEPQYRYLNPRCKCGKRMKSAGKDKGYKCPRCSDRITDVPKEKMEV
                     QRNLEKGYYEVPPSARRHLSKPLIRIQRHK"
     gene            2887..4194
                     /locus_tag="PKK85_01745"
     CDS             2887..4194
                     /locus_tag="PKK85_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HNS24777.1"
                     /translation="MITEKLAHFIHYTGYRDFPSEVVKQAKLCFLDFLGVSIAGSRTR
                     SGQVMRSTISSRGSSTVIGGPKTSALEACLINGVSAHSLDLDDGHRLAQLHPGACVIP
                     AALSLAESVGVSGIELLEALILGYQVAISLGMMVNPSHRNRGFHSTGTCGTFGAAAAA
                     SKVLNLSEEEIINCLGLAGTQAAGLLESNHSGSMAKHLHPGRAAHSGVFSALLAQKGF
                     TGAPSILEGREGFLKAMCQVDTQQSEWGTILDSHYHIMGVYFKNYPVCRHLHSALDAT
                     FYLLRKYKINPHDVVELEVKTYQVAAEHQDYIPQSSEAIRQSLPVALALALSQGHLDP
                     HGLYTNSEVLSLASKVSVKCNSEFEALYPGKRPSRVIINTNKGSFKHTVYLPRGEPEK
                     PFSWDDLLSKFQRLNPKFDEDTLGIIKKMDSYRARDLMNILLN"
     gene            4221..4924
                     /locus_tag="PKK85_01750"
                     /pseudo
     CDS             4221..4924
                     /locus_tag="PKK85_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019267646.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         1391 a         1046 c         1148 g         1339 t
ORIGIN      
        1 gaccagggtg attgggattt cattgccgat aacttgaacc acatcaggga gacagaagct
       61 attcccgggt tggtgaccca tcgccccttc cacaccacca gcaagctcct aaaatctcct
      121 gttaaagatc tttttgaact gtacatgata ccagtcaacc ggctgggtta cctgatggac
      181 tgtgaacaga acctggaaga ggagcgaaaa ctactcaggg aaatggttct gaagctggac
      241 aaaaccataa tcgggaaaaa attactggcc gctgggataa tgactcccca tgacgctttt
      301 gaatacctga aactgctgga ctacgtggac attgtggcag tgggtattgc ctcggcaaag
      361 gaggcccatg aaacattcgg ggttctttct aaaaaataat atgtatacaa gggacccttt
      421 agtatgcgat gctagctatc ccttctttct tttaagagtt tcatgaagtc ctgggaactt
      481 ttaagttctt ccatctccca gttatgcacc accggcactc cctctatgga cttcaagttc
      541 tgccggttgg ccagtataaa tgcggcttca gtgtcggtga tggatgagat gtccctcaaa
      601 ttcatggcca tcttggccag taacttctga ttcctctgct tatccaggtt agctattata
      661 ggctgagcat ccttagattt actctgatta ggtcttgcta aagcatcgaa tggggttcga
      721 ttagtcttta taaccccaaa gcccaattct tccagttccc tgtgcttgga ttctttaatt
      781 tttgcatcct gcgtggttac agggacttgg aaaagattca ccgacacggt gatcttcatg
      841 ttcagtattc gctccagaat cattgctgtt tcaggatggg cccggaccct accagtttca
      901 tatttataaa tcgtctcccg tgagacatgg accagatcag ccagttcctt taaggacaaa
      961 tcctgttttt cccggagttc cttaatgatc tggccatcaa tttgtacata atacccccct
     1021 cggccagcga atacttcagg atagagattg tcaataatca ggttgcgaaa ggtttccatt
     1081 ccaataacag ggatgccatg acgctcatag accacatctt cttccaagta ctcgttttta
     1141 gattttgccc ccaccaccag tggtgatgcc aggagggtgt tggccaccat ctttatttcc
     1201 cgggcttgtt ccatgctaaa accatccaca tttaccaaaa ccttcattaa aagaagtatt
     1261 aattctcgtc gggcaaccag gtcaaagcaa ctcctatcat atatattaga agtttcaaaa
     1321 ccatggttgg tcaatgtctt agtgatgtct aacagtagtt cttcccggtg cactgggagg
     1381 ttcgcatgtg gcagatgatc accccggtga tgcaatgtac aacaatatat tacatgtggg
     1441 ttttgatgac acagattcca gcagtggaat gtgtaccact tatgtatgtg ctgtggttct
     1501 ggagagactt aaagcttgtg gtatgaccat aagaggagaa gctcgcctga tacgtctcaa
     1561 cccatttgca ccctataaaa ccagaggaaa tggtgcggta tccttcagtg tagttttgga
     1621 tggtgctggg gtggttgaaa gagttaaaaa gatagtttta gctacggtgg aagagtattc
     1681 acagatggac gacccctcca ccaacccggg agtggtgttt taccatggcc ccatcaccga
     1741 agatctgcag gaatattcgt tacggactgt gcagtctatg gttaccctgg aagaggcaga
     1801 gaaactcgcc caaaagattg aagcagaagt gtttaaatat aaaaatggta ggggagtgat
     1861 tggggcttta gctgccattg gatgtccatt aatagataaa acctatgaat taattgcata
     1921 ccgggaagtt cataactacg gtaaagtccg tagagtaaat gaggattcag tgcgtttgat
     1981 ggatcgtgcc acttatcctt ctacctttga taacttgtat gaggaccata taaccatcac
     2041 accccacaca ccatgtcctg tgctttatgg gattagagga gagagtaaag aggctgtgta
     2101 tagggctcat aacctggtaa aatctgagga accaattgaa cggtttatgg tgtttttaag
     2161 caaccaacac actgacatgc acctccaaaa tactgatggc attgctggaa tggaacagta
     2221 tcaatgttac cgggtagcag gtgaagttaa cagcaccccc tacgtaatcg aagggggaca
     2281 cgttattttt aacttgaaag acaaatccgg agagatagaa tgtgcagcct acgaacccac
     2341 taagggattc cgggatacag tacgtggact tgctcctggt gatgatctta ttgtttatgg
     2401 aggaattgga gataaaggaa ctttaaatgt ggagaaaatt caaattcttc atttggagcc
     2461 acagtaccgg taccttaatc cccgttgcaa gtgtggtaag cgcatgaagt cggctgggaa
     2521 agataaagga tataaatgtc cccgttgcag tgataggata actgatgtcc caaaagaaaa
     2581 gatggaagta cagagaaacc tggaaaaagg atactatgaa gttccgccat ctgcaagaag
     2641 acacttaagc aagcccctga tccgaatcca gagacataaa tgacactacc ctgtgcttgt
     2701 ctgagtaacc agcccacacc catcttctac taatttttag aggaattctg ttttcaactg
     2761 cagtttatgg gcctcatcgt gtctacctga cttgaagtgg cccaattcaa aatctaatct
     2821 gaatttctag ttaccttgat atcaaaaagt ttatcatcat cgttgctatc cctaggataa
     2881 tataatatga tcactgagaa actggcccat ttcattcatt atactgggta tagagacttt
     2941 ccctcagaag ttgtaaaaca agcaaaactg tgttttctag attttttagg ggtttccatt
     3001 gctggctcaa ggacccgcag tggtcaggtg atgcgaagta ccatcagttc caggggctcg
     3061 tccacggtta ttggcggccc aaaaaccagc gccctggagg cctgtctaat taatggcgtg
     3121 tctgcccata gcctagatct agatgatgga catcgcctgg cccaattgca ccccggtgcc
     3181 tgtgtgatac cggctgccct atcactggct gaatccgttg gggtaagtgg aattgagctt
     3241 ctagaagccc tgatcctggg ataccaggta gcaatatcac tgggcatgat ggttaatccc
     3301 agccacagaa acaggggatt tcacagtacc gggacttgtg gaacatttgg ggcagcggca
     3361 gcagccagta aagtgttaaa tctgtcggaa gaagaaatta taaattgttt aggtctggcc
     3421 ggcacccagg cagcaggttt actggagtcc aaccattccg gcagcatggc aaaacatttg
     3481 catcctggcc gggcagctca ttccggagta ttttcagcac tactagcaca gaaaggattc
     3541 acaggagcac ccagcatatt ggagggacgg gaaggttttt tgaaagccat gtgccaggtt
     3601 gatacccagc aaagcgaatg gggaacaata cttgattccc attaccatat aatgggagtt
     3661 tacttcaaga attatccagt gtgcagacat ttacactcgg ctttagacgc tacgttctat
     3721 ctacttagga aatataaaat aaatccccat gatgttgttg aattggaagt taaaacttac
     3781 caagtagcag cagaacacca ggactacatt ccacagagtt cagaggccat cagacagagt
     3841 ttaccagtcg cgctggccct ggccctttca cagggacatc tagatcctca tgggctttac
     3901 actaacagtg aagtattgag tttagcatcc aaggtgtctg tgaagtgtaa ttcagaattt
     3961 gaagcacttt atccggggaa aagaccatcc agagtgatta taaatacaaa taaaggttca
     4021 tttaaacata cagtttattt acctcgagga gaaccggaaa agcccttcag ttgggatgat
     4081 cttctatcta aatttcagag gttgaatcca aaatttgatg aggataccct gggtataatt
     4141 aaaaagatgg attcatatcg tgctagagat ctaatgaata tactgctgaa ttagggtaaa
     4201 gaagatttaa aggtggttta atgaacacag tggattattt gaaggatatt ggtataaata
     4261 ttgatgataa tagctcatca gattcttcta agctctttga ggatggtggc cagtaccgtt
     4321 ttgaagtgcc gggaatccaa aaaccagtgg cactggcaag tttgattgat gctctagacc
     4381 actacgaggt tcaaattcat cgtgtgactc agactaaagg tataatgtta cttactgacc
     4441 aggaaatact ggaaatgact gatttagccc tagatgctgg tttagaactg tttttaagtg
     4501 ttggaccccg ggctacatat gataccagtg cttcagccca gacccaagag ggggccagga
     4561 ttgggtaccg tctgcggggt taccagaatt tgttgtacgc catagaggat gttaagcggg
     4621 cggtgcatct gggagttagg gggatcgtcc tgtacgatga gggcctgctg tgggtgctat
     4681 aaaagatgcg tagcgatgga atacttccct ccaatgttca tttcaagatt tctgcccaca
     4741 ctggccatgg aaatcctgca tctgctcttt taatgcagaa tataggggca aattccttta
     4801 atccggtgcg agacttatca ttgaacatga tgaatgagat ccggcaagtc attgacatct
     4861 cccttgatat acacaccgaa aaccccaaat cctctggagg atttataagg cattatgagg
     4921 ttcc
//
LOCUS       DAOQVW010000046         4905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_19095_length_4905_cov_3.316289, whole genome shotgun sequence.
ACCESSION   DAOQVW010000046 DAOQVW010000000
VERSION     DAOQVW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19095_length_4905_cov_3.316289"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..354
                     /locus_tag="PKK85_01755"
     CDS             118..354
                     /locus_tag="PKK85_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24778.1"
                     /translation="MDGEVVGALSYSWGPDPGCGFYYGTGNCSPGLKISWILGWVLVV
                     EGLARIPPNAGVKACGFKDLILYNDPSVGNVHNI"
     gene            518..1507
                     /locus_tag="PKK85_01760"
     CDS             518..1507
                     /locus_tag="PKK85_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS24779.1"
                     /translation="MKAIETVNLTKKFGTLTAVDQVNLEVDQGEIFGLLGPNGAGKST
                     FISMLCTILKPTSGTAKVEGYDVTTEPGDVRRSIGIVFQDPSIVDKLTGMENMELHAD
                     LYDVPRDVMHTRIDEVLQLVQLKNRASNYVNTYSGGMRRRLEIARSLIHYPKVLFLDE
                     PTVGLDPQSRDHIWNYIRELKKQESITIILTTHYMEEADKLCDRIAIIDKSRIIAMDT
                     PQNLKNELGGETILIHSSDNDLLNRKITEAELAETILQTENELNLCVKNAHNNVARIV
                     ELAVSVGVHIDSITIREPDLNDVFMHFTGREIRDGSGGKALPGMGARIMGGIK"
     gene            1531..2259
                     /locus_tag="PKK85_01765"
     CDS             1531..2259
                     /locus_tag="PKK85_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS24780.1"
                     /translation="MWRRELKRFIRDHSRLVSSVISPVLWLVIFGGGLGFSIADATGL
                     NYTQFLLPGIISQTLLFTAMFLGISVIWDRQFGFMKEILVAPINRISIFTGKMLGVGS
                     AALIQGLIVVALGFVIGVPLTWYAMSLAIPLMIIITIGLTCIGLIIASLMSTLESFGT
                     IVTFVNMPMFFLSGALFPVTNLPQWIRWAFYINPLTYGVDALRAVMIPGWHTLLPLQL
                     EVLVIVVFDVLMIVMGTYFFSKRQ"
     gene            2344..2583
                     /locus_tag="PKK85_01770"
     CDS             2344..2583
                     /locus_tag="PKK85_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24781.1"
                     /translation="MDCKVEVSNETGRNVCKFEDDKFEVTIVLKKDYSDVEVKLRTSK
                     ILKDIEEALEVVTRTDEFKDLVDSYGYLNIERIEG"
     gene            complement(2825..4528)
                     /locus_tag="PKK85_01775"
     CDS             complement(2825..4528)
                     /locus_tag="PKK85_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HNS24782.1"
                     /translation="MDTEKFVLLDIDYVTRYGQPVIRLFGKIPVDNRSIIAHDPNFHP
                     YLYLLPYDLEACREELVPFEVPRIERLRRIDKDGVVKEFLKLTLRHPRDLLPLKRQME
                     NLKSVESVREYDIPLPQRYLIDRGLYPLQEVEVQGRVLKNSGATCLMEIQKNPRPVES
                     GLPGLKILSFKIETHIHPRLRTDPILHIGLSSNQGYQKVLSTTKSSSSLVESLPTEKE
                     LLEKFAETIRSLNPDIITGYNSDKYDFPYLQGRADLLGVNLNLGVDGSPVKFRTLPKK
                     SAAIKGRVHIDLYRVARRHLQLTDHTLRNVYQELYREEKMDISLDQLHTYHGHEKLLH
                     YSLEDTQAIHKIGEKMLPLVMEVARLVGQSLFEVTRRGSSYQVECLLMRKAYQYGYVV
                     PNKSGTYFQDVVGGHVEEPVPGLHENIFYFDFRSLYPSIIVAKNISPDTLTESDVEEC
                     HVAPEYGYRFRKEPAGFIPRVTSQVLKERIRIKDKMKETSDPGKRQVLDFRQRVLKIF
                     ISTIYGLFNHSTYRWYSAEASEAITAWGRDYLKKTMEDAEKQGFRVLYADTDGFYATM
                     N"
     gene            complement(4539..>4905)
                     /locus_tag="PKK85_01780"
     CDS             complement(4539..>4905)
                     /locus_tag="PKK85_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24783.1"
                     /translation="KWHGYLKEFLDGLYQLDFHERGKGKPVSLTDLNLALGVVNNIAE
                     EWEALFDKEHEPDPSYQYLSTLDEKYKRPRSSYTSDMHRVDLQVPVINPELRYRHQLE
                     MVEEANQEFTRALKEKKNP"
BASE COUNT         1213 a         1156 c         1227 g         1309 t
ORIGIN      
        1 cctctagact tcgggttcat ctcagcccag ccctgggacc agtggctgcc atatcaggat
       61 tcttaatcac caatatgggg gggtgcactg ttctcatcct attaggaata gggggaagtg
      121 gatggtgaag tggtgggagc actctcctat tcctggggcc ctgatcctgg atgtgggttt
      181 tactatggca cagggaactg ttcacctgga cttaagattt cttggatcct gggatgggtc
      241 ctggtagtgg agggattggc cagaattcca cccaatgccg gggtgaaggc ctgcggattc
      301 aaagacctaa tcctgtacaa tgatccatct gttgggaacg ttcataacat ctaaggactg
      361 gatttaggtg gtacaaattg gttcatggct attagccaca gggataagtc tccttggaaa
      421 gtgtggggag tagataaaaa tcatttagtt tattatgggg ggagataaaa tacttctgta
      481 acaccccacc accaaataaa ctatagggag atgattcatg aaagcaatag agactgtgaa
      541 tttaaccaaa aaattcggca cactcaccgc cgtggatcag gtgaacctcg aggtggatca
      601 gggggaaatc ttcggtttac taggccccaa tggcgctggt aaaagtacct tcatctccat
      661 gttgtgcact atactaaaac ccacatctgg cacagctaag gtggagggat acgatgtcac
      721 cactgagccc ggggatgtgc ggcgctctat agggattgta ttccaggatc ccagtatcgt
      781 tgataaactc actggcatgg agaacatgga gctccacgct gatctctacg atgtgccacg
      841 ggatgtgatg cacaccagga tagatgaggt tctgcaactg gtccagttaa aaaaccgtgc
      901 atctaattat gttaacacct actccggtgg tatgaggcgt agactggaga tagccaggag
      961 tttgattcac taccccaagg tactgttcct ggatgaaccc acagttggcc tggaccccca
     1021 aagccgtgac catatctgga attatatacg ggaactgaag aagcaggaga gcatcaccat
     1081 catcctcacc acacattaca tggaggaagc agataaactc tgtgacagga ttgcaattat
     1141 tgacaagagc aggattattg caatggacac tccacaaaac cttaaaaacg agctgggtgg
     1201 agaaacaatt ctcatccact ccagtgataa cgatctttta aataggaaga tcactgaagc
     1261 tgaactcgcg gaaaccatcc tccaaactga gaatgaactt aacctgtgcg ttaagaatgc
     1321 tcataacaac gtggcccgta tagtggagtt ggcagtatca gtaggggtgc acatagacag
     1381 tatcaccata agagaacctg atttaaacga tgtttttatg cacttcaccg gccgggaaat
     1441 tcgtgacgga agtggtggca aagcactccc gggtatgggt gcccgaatta tgggggggat
     1501 aaaataagcg aaatcagggg tataactgca ttgtggcggc gtgaacttaa acgtttcatc
     1561 cgggaccatt cccgactagt tagttctgtg atctcgccgg tactgtggct cgtaattttt
     1621 ggtggaggtt tgggattttc cattgcagat gcaacgggcc ttaactatac ccagtttctc
     1681 ttacccggca ttatctccca gacccttctt ttcaccgcca tgtttctggg tatttcagtg
     1741 atctgggatc gtcaattcgg ttttatgaag gaaatcctgg tggcccccat taaccggatc
     1801 tccatcttca cagggaagat gctgggagtg ggttctgctg ccctaattca gggactaatt
     1861 gttgtcgccc taggatttgt tattggcgtc cccttaacct ggtacgccat gagcttggca
     1921 atacccttaa tgatcatcat aaccataggt ttaacctgca ttggcttaat tattgcaagt
     1981 ctaatgagca ccctggaaag cttcggaacc attgtaacct tcgtcaacat gcccatgttc
     2041 ttcctaagcg gggcactgtt cccagtcaca aaccttcccc aatggataag gtgggctttc
     2101 tacattaacc cactgaccta tggtgtggat gccctgcgag ccgttatgat cccagggtgg
     2161 cataccctgc tacccctgca actcgaagta ctagttatag ttgtctttga tgtgcttatg
     2221 atcgttatgg ggacctactt cttcagtaaa agacagtaga tcccaaactt gtgaattgga
     2281 tctggtctgt accaaaaaat gtttaatgtc acagaagtga tctaattaac aaggggtgat
     2341 accatggatt gcaaagtaga agtttctaat gaaaccggcc gaaatgtttg taaattcgaa
     2401 gatgataaat ttgaagttac catagttctt aaaaaagatt attctgatgt ggaagttaaa
     2461 cttagaactt caaagatcct caaggacata gaagaggcat tagaggttgt gacccgcact
     2521 gatgaattta aggatctggt ggatagctac gggtacctca acatagagcg cattgaggga
     2581 tagttccggc accatattca ggctgcccca tcatctattc taaaaaagca cctgtgtaca
     2641 gggttgctgg ccatgtaaga tccccatata acctatcatc ttagaccaga acactgacac
     2701 ctgggataca cctagtttcc agtactatca gcccagaaag ggtgttcaaa tcaccccagg
     2761 gcatagataa ctggatacgc accataaaaa atgggggggg gactgttaaa acaagcacat
     2821 ccccctagtt catggtggcg tagaatccat cggtatcagc gtagagcacc ctgaagccct
     2881 gtttttctgc atcttccatg gtttttttca aataatcccg cccccaggct gtgatggcct
     2941 ccgaggcctc tgcactgtac cagcggtagg tgctgtggtt gaagagtcca tagatagtgc
     3001 tgataaatat tttcagcacc ctttgcctga agtccaggac ctggcgtttc ccggggtcgc
     3061 tggtttcctt catcttatcc ttgatcctta tccgctcctt cagaacctgg cttgtgaccc
     3121 ggggaatgaa cccggctggt tctttccgga atcggtatcc atactctggt gccacatggc
     3181 actcctccac atcactttct gttagggtgt caggggagat gttcttggct acgatgatac
     3241 tggggtacag gctgcggaag tcgaagtaga agatgttctc gtgcaaacct ggaactggct
     3301 cttccacgtg gccacccacc acatcttgga aataggttcc tgacttgttg ggcaccacgt
     3361 acccgtactg gtaggctttc ctcattaaaa ggcactccac ctggtagcta ctgccccgcc
     3421 tggtcacctc aaagagggac tggcccacca gccgggcaac ttccatgacc aggggcagca
     3481 ttttctcccc gatcttatgg atggcctggg tgtcctccag ggaatagtgt aggagtttct
     3541 catgaccatg gtaggtgtgg agctgatcca gggagatatc catcttctcc tccctgtaaa
     3601 gctcctgata cacgttccgg agggtgtggt cagttaactg aaggtggcgg cgtgccaccc
     3661 ggtaaagatc aatgtggacc cgtcccttta tggctgccga tttcttggga agggtcctga
     3721 atttcacagg ggacccatcc actcccaggt taaggttcac acctaggagg tccgcccggc
     3781 cctggaggta ggggaagtcg tatttgtcgc tgttgtagcc ggtgattata tctggattca
     3841 gggatctgat ggtttctgca aatttctcta gtaactcttt ctcggtgggg aggctctcca
     3901 ccagactaga tgaggacttc gtggtggaga gtactttctg gtagccctgg ttactggata
     3961 ggccaatatg gaggatggga tcagttctta accgggggtg gatatgggtt tcgatcttga
     4021 agcttaggat cttaagtcct ggtaaaccag attcaactgg ccggggattt ttttgtattt
     4081 ccattaggca ggtggcaccg gaattcttca acaccctccc ctgcacctct acctcttgga
     4141 ggggatataa tcccctgtca atgaggtatc tctgggggag gggtatatca tattcccgga
     4201 ctgattctac cgatttcaaa ttctccatct gcctctttaa agggagcaga tcccggggat
     4261 gtctgagggt taattttagg aattccttca ctacaccatc cttatctatc ctgcgcagcc
     4321 tctctatcct gggaacttca aaggggacca gctcctccct gcaggcttcc aggtcatagg
     4381 ggagtaggta gaggtagggg tggaagttag ggtcatgggc gatgatggac ctgttatcta
     4441 cgggtatctt cccgaataag cgtataactg gctggccata acgggttacg tagtcaatat
     4501 ccagcagtac gaatttctca gtatccataa accatccctt atgggttttt tttctcttta
     4561 agggcccgtg tgaattcctg gttggcctcc tccaccatct ccaattggtg tctgtatctc
     4621 agctccgggt taatcacagg gacctgcagg tcaacccggt gcatgtcact ggtgtaggag
     4681 gatcttggcc ttttgtactt ctcatccagg gtggacaggt actgatagga aggatcaggc
     4741 tcatgctcct tatcaaagag ggcctcccac tcctcggcta tgttgtttac cacacccagt
     4801 gccaggttca aatcggtaag gctaactggt tttccttttc ccctctcatg gaagtccagc
     4861 tggtataaac catccaggaa ctccttaaga taaccatgcc acttc
//
LOCUS       DAOQVW010000047         4886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_19209_length_4886_cov_3.523080, whole genome shotgun sequence.
ACCESSION   DAOQVW010000047 DAOQVW010000000
VERSION     DAOQVW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19209_length_4886_cov_3.523080"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..594)
                     /gene="msrA"
                     /locus_tag="PKK85_01785"
     CDS             complement(103..594)
                     /gene="msrA"
                     /locus_tag="PKK85_01785"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNS24784.1"
                     /translation="MMNKEEVVLQKATFGAGCFWGVEAAFREVKGVISTVVGYMGGDL
                     DNPSYQDVCTGTTGHSEVVEVIFNSHEVSYRDLLHHFWSIHDPTTVNRQGPDVGVQYR
                     SVIFYHDLDQKKLAEKMRDELQKSNIYPRDIVTAIEPAQTFWKAEDYHQQYFEKTKRR
                     SCF"
     gene            737..2098
                     /locus_tag="PKK85_01790"
     CDS             737..2098
                     /locus_tag="PKK85_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS24785.1"
                     /translation="MSSTNKNYALIVTVIASFLTPFMGSSINIALPTIALEFKANAIL
                     LGWIPTVYLLSLAVFLVPFGRIADIYGRKRVFTYGIIIFTISSLVAGFSISTGMLLLL
                     RVFQGLGAAMIFGNVAAIIASLFPPGERGRALGISVTGVFLGLFLGPVLGGFLTQNLG
                     WRSIFFLNVVLGLLSTLAVLRLRGEWTEAQGEKFDLPGSLVLGMSLVSLMYGLSELPT
                     LHGFFLILGGLLGLVLFFSVESRMKSPVLEVKLFKNKIFTLHNLAAFINYCAGVPIVF
                     VLSLYLQYIRALDPQSTGLLMAVQPVTMAIFSPLAGKLSDRAEPRLVASAGMAFTALG
                     LAFLAFLTQETSLFWIISGLIILGLGFALFSSPNTNVIISSVNSRYYGVASATLSTMR
                     VLGQMSGMGLAIFTLAIFVGSASIQPDNYGRFLLSARISFALFSFMSLLGIIASLSGK
                     KEN"
     gene            2151..3854
                     /gene="polX"
                     /locus_tag="PKK85_01795"
     CDS             2151..3854
                     /gene="polX"
                     /locus_tag="PKK85_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNS24786.1"
                     /translation="MENKKVAAILDRVADYLEMDGADFRTRAYRRAAHTVDFLPQPIE
                     EVRKEGGLEKLPGVGKAIALKIKEILDTGSLQYLEDLEKQYPVDFEGLLLVEGVGPKT
                     IKLLYQELGVKNLADLEKEAKSGHIQHLRGMGIKSEKRILKNLEFAKKSTGRRLLGDI
                     MPLALELKEKIGSLPEVEIVEIAGSIRRKKETIGDIDVLAVTSDPQKVMDYFVNMDLV
                     GGIISRGPSRSTIRLKDGLEVDLRVFRKKEYGSALVYFTGSWELNVELRKMAIGRGLK
                     LNEYGIFRGEEQLAGETEEEVFRALGLSYIEPELRENQGEIEAALTGELPKLVETSDI
                     KGDLHIHTQWSDGRSSILEMALAAQKLGYQYLAITDHAHGLPVAGGLDETALMEQIRE
                     IDRVNDELEGIRVLKGVELNIRADGTLDIDTVVLEELDIVLAAIHSGVPFLPGTLTKI
                     IQDTVENEHVDILAHPSGRKLKKRPAYDIDLEKVFETAADTGTIMEVDGQPNRLDLSD
                     ENIRTALKYGCKLSLDTDAHHPQDLQHMELAVFTARRGWAEARDVVNTLPLNKLLKTL
                     K"
     gene            complement(3872..4111)
                     /locus_tag="PKK85_01800"
     CDS             complement(3872..4111)
                     /locus_tag="PKK85_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNS24787.1"
                     /translation="MYPARYNWKSQLPNCQLQTLERYLFDLERVDDVPGHLVPDYYRT
                     YLHEGNIGPLVPILEHNRMDVVTLIRLLSLIQQDI"
     gene            complement(4051..4815)
                     /locus_tag="PKK85_01805"
     CDS             complement(4051..4815)
                     /locus_tag="PKK85_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNS24788.1"
                     /translation="MSKLTDNPEELKKKLLLKYQDQSLEDLSHGTEVENIHGTCFSFK
                     KKEKIKIKTPSIEKIKKQILSDLKLLNGVGQTKESKLKENGYQTLRDLVGHDRYGGEA
                     ASIVKLLDEKDFDKLSEHITRVFFPSHPLNTLCISTRDPEDFLFMDIETMGLDGVPLI
                     LIGVARTSGSKVETIQFLLRDLNEEKAALESYFSLYHDKTLLVSFNGRSFDAPFIRRR
                     MKHHHLDILTQPIIWIYCIQPGTTGSRNFPTASSKP"
BASE COUNT         1258 a         1076 c         1182 g         1370 t
ORIGIN      
        1 gagcctcagt atctgaaaaa tctattaaac attctaaagc cccatgatat gggcagaaag
       61 ccttgcttat ggagtttata ctagactggc tttggaatgg gactagaaac aactccttct
      121 ttttgtcttt tcaaagtact gctggtggta atcttccgcc ttccagaagg tttgggctgg
      181 ttcgatggct gtaacaatat ctcggggata aatatttgac ttttgaagtt catcacgcat
      241 tttttctgcc aactttttct gatccaagtc gtggtagaag atcacggaac gatattgtac
      301 ccctacatcg gggccttgac ggttaacagt ggtggggtcg tgaatactcc agaaatggtg
      361 cagtaaatcg cggtaagaaa cttcatggga gttgaagatc acctccacca cttcgctatg
      421 accggtggtg ccggtgcaaa catcctggta tgaagggtta tccagatctc ctcccatata
      481 accgacaaca gtggaaatca ctccctttac ttcacggaat gcggcctcaa ccccccaaaa
      541 gcatcctgcc ccaaaggtgg ctttctgaag tactacttcc tctttattca tcatatctat
      601 cctcctggtt gaatgaagat ggtggttggt gtttttaccc attcagcact ctttttggtg
      661 tttatccctt ttttgtagat gaacagctat agttatctat atccataaat cacattaaat
      721 tttaggtgaa tctattttgt ccagcaccaa taaaaactac gccctgattg taactgttat
      781 agcatcattt ttaacaccat tcatgggttc ttccataaac attgctttac ccaccattgc
      841 cctggaattt aaagctaacg cgattttatt gggatggata ccaactgttt atcttttatc
      901 cttggcagtt ttcctggtgc cattcggccg aattgccgat atatatggta ggaaaagggt
      961 tttcacctat ggtattataa ttttcaccat atcctcattg gtagctggtt tctccatctc
     1021 caccgggatg ctcctacttt taagggtctt ccagggactg ggtgcagcta tgatttttgg
     1081 taacgtggct gccattatag catccctttt tcctccagga gaacgtggaa gagccctggg
     1141 aataagtgta actggcgtat ttctaggtct atttttgggt cctgtcctgg gagggttcct
     1201 gacccagaat ttggggtgga ggagcatatt ctttttaaac gtcgttctgg gccttctttc
     1261 caccctggct gtcttaaggc taaggggtga atggactgaa gcgcagggag agaaattcga
     1321 tcttccaggg tccctagtat tgggaatgtc cctggtgtcc ctgatgtatg gcttatcaga
     1381 acttcccacc cttcatggtt tcttcctgat tctgggcgga ttactgggat tggtactatt
     1441 tttttcagtg gaaagcagga tgaaaagtcc tgttctggaa gtaaaactct ttaaaaacaa
     1501 gatcttcacc ctgcacaacc tggctgcctt tataaactac tgtgccgggg tgcctatagt
     1561 cttcgtttta agtctctacc tgcagtacat cagggccctg gatccccaga gcaccggtct
     1621 tttaatggcg gtacaaccgg taaccatggc tattttctcc cctctagctg gtaagctatc
     1681 ggaccgggcg gaaccccgcc tggtggcttc ggcagggatg gccttcactg ccctgggctt
     1741 ggccttcttg gcatttctga cacaagaaac cagtcttttc tggatcatca gtggcctcat
     1801 aatactgggt ttgggatttg cccttttttc ctcaccaaac accaacgtca taataagcag
     1861 cgtcaattca cgctattacg gggttgcttc cgctacttta agcaccatgc gggttctggg
     1921 ccagatgtca ggtatggggt tggcaatttt caccctggcc atcttcgtag gtagcgccag
     1981 catccagcct gataattacg gccgcttcct gttaagcgcc aggatatctt ttgccctgtt
     2041 ttcttttatg agtcttttgg gaataatagc ctctttatcg ggtaaaaaag aaaactaaaa
     2101 cccttacaat gggggaagaa gtttttaggg ggaaccacat atatatggtc atggagaata
     2161 agaaagttgc agctatcctg gaccgggtgg cagattacct ggaaatggat ggtgctgatt
     2221 ttcgtaccag ggcctaccgt cgtgccgccc ataccgttga cttcctgccc cagcccatcg
     2281 aggaggttcg aaaagagggg ggcttggaga agctgcccgg tgttggaaaa gccattgccc
     2341 ttaagattaa ggagatcctg gataccggat ccctccagta cttggaggac ctggagaaac
     2401 agtatccagt ggacttcgag ggcctgctcc tggtggaggg agtgggcccc aagacaatca
     2461 aactactcta ccaggagctg ggagttaaaa acctggcgga ccttgaaaaa gaggctaaaa
     2521 gtggccatat acaacacttg aggggtatgg ggattaagag tgagaaaagg attcttaaaa
     2581 acttggaatt cgctaaaaag agtactggaa ggcgactttt aggtgatatc atgcctctag
     2641 ccctggaact taaggaaaag ataggttcac tacctgaagt cgaaatagtg gaaattgcag
     2701 gttctatacg ccgaaaaaag gaaaccattg gggatattga tgttttagcc gttaccagtg
     2761 acccccaaaa agtgatggac tacttcgtta acatggactt ggtgggtggg ataatatccc
     2821 gcggcccctc ccgatccacc atccggctta aggatggact ggaagtggac ctgagggtgt
     2881 tcagaaagaa agaatatggt tcggccttgg tgtatttcac ggggtcatgg gaacttaacg
     2941 tggagctgcg aaaaatggcc ataggccgtg ggcttaaact aaatgaatac ggaatattca
     3001 ggggggaaga gcaattggcc ggggaaactg aggaggaagt tttcagggcc ctgggattgt
     3061 cctacattga accggagcta agggagaacc agggggagat cgaggcggca ttgactggtg
     3121 aacttccgaa actggttgaa acatctgata taaaagggga tcttcacatc cacacccagt
     3181 ggagtgacgg ccgctcatcc atactggaaa tggccctggc cgcccagaaa ctgggttacc
     3241 agtacctggc tattacagac catgcacatg gccttcctgt tgccggtgga ttggatgaaa
     3301 cagccctaat ggaacaaata agagagatag atagggtgaa tgatgaactt gaaggtatcc
     3361 gggtgcttaa aggcgtggaa ctaaatatta gagcagatgg tactttagat attgatacgg
     3421 tagtcctgga agaattggat attgtgctag ccgcgattca ttcaggagta cccttcctac
     3481 ctggaaccct gaccaagatc atccaggaca cagtggagaa tgaacacgtg gatatcctgg
     3541 cccacccctc gggcaggaaa cttaaaaaac gacccgccta tgacatcgac ttggaaaaag
     3601 tctttgaaac cgctgctgat acaggaacca tcatggaagt ggatggacag cccaatcgtc
     3661 tggatctgag tgatgaaaat ataagaaccg cccttaaata tgggtgtaaa ctgtccctgg
     3721 ataccgatgc ccaccacccc caggacttgc aacacatgga actggcagta ttcaccgccc
     3781 gccggggatg ggcggaagcc cgggacgtgg ttaacactct ccccttgaat aagcttctca
     3841 agactttgaa atgaatttta tgataaaaat cttatatatc ctgctggatc agggataata
     3901 gccttatcag ggtaaccaca tccatccggt tatgttccag gatgggtacc aggggtccta
     3961 tattaccctc atggaggtag gtgcggtagt aatcaggtac caagtggccg gggacgtcat
     4021 ccacccgttc cagatcaaaa agataccttt ctagggtttg gagctggcag ttgggaagtt
     4081 gcgacttcca gttgtacctg gctggataca gtaaatccag atgatgggct gggtcaggat
     4141 atctagatgg tgatgtttca tccttcttct gatgaaggga gcatcaaaac tccgaccatt
     4201 gaagctcacc agcagagttt tgtcatggta aagggaaaag taactctcta atgctgcttt
     4261 ttcttcgttc aaatctctca ggaggaactg aatagtctct accttgcttc cggaggtcct
     4321 ggccactccg atgagtataa ggggcactcc atccagtccc atagtttcga tgtccatgaa
     4381 taagaagtcc tctggatctc gggttgaaat acacagggtg ttaaggggat gggatgggaa
     4441 aaataccctg gtaatatgct cggaaagttt atcaaagtct ttttcatcca gtaacttaac
     4501 aatactggca gcttcccctc cgtatctgtc atgtccaacc agatctcgca gggtctggta
     4561 accattttcc ttcaatttag actctttggt ctgacccacg ccatttagaa gttttaaatc
     4621 ggataatatc tgttttttta tcttttcaat tgagggggtc ttaattttga ttttttcttt
     4681 ctttttaaag gagaaacatg tgccatggat gttttcaacc tcagttccgt gggataaatc
     4741 ttctagggat tgatcctggt acttcaaaag taactttttt ttaagttcct ctggattatc
     4801 ggtcagcttt gacatctatt ttttacatcc ccttttcata tcatattata cagtcttgtg
     4861 accaaaactt tgacaaaaat tatatg
//
LOCUS       DAOQVW010000048         4863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_19314_length_4863_cov_3.445507, whole genome shotgun sequence.
ACCESSION   DAOQVW010000048 DAOQVW010000000
VERSION     DAOQVW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19314_length_4863_cov_3.445507"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKK85_01810"
     CDS             <1..225
                     /locus_tag="PKK85_01810"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit beta"
                     /protein_id="HNS24789.1"
                     /translation="TRTDAGETIFKAAVDAGVIETKAMDDVKPGLGMLEKLATDKKTK
                     AMKVIDDRKAMGLPVPYKASSEKEDPLANV"
     gene            376..1398
                     /locus_tag="PKK85_01815"
     CDS             376..1398
                     /locus_tag="PKK85_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNS24790.1"
                     /translation="MKSESDKRSCPRCGAFKVENARFCVQCGTSLDNYPQITCVICST
                     CGTENPSYASFCMECGVDLKVTNRKTEEDGHFPDKMGSLELTPAESLLILDLDIEGKE
                     VFKLAMLNLIDRRVMKIRTFQEDKGFLVKEPVLFHEVHRGENFEAPLLPYEEIFRKPL
                     YSHNEIEIYRYVQMILKNINPIVTNSYSEYKEDYLINSLIKGGYIEKIGKKGLNSPKP
                     YKLTQLGVDIRKKIVDILDDADNLGEWVDTDPKRAKAFLSALGSHIFLLNYDLDTLRF
                     LTNNLTGIESRTAKYYSYYLSPIDFFKDFKSEQDIKYIFRINALESFEFFETFDPFDD
                     IFDSFE"
     gene            complement(1756..2676)
                     /gene="map"
                     /locus_tag="PKK85_01820"
     CDS             complement(1756..2676)
                     /gene="map"
                     /locus_tag="PKK85_01820"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HNS24791.1"
                     /translation="MIENYIKAGKIVKDVRELALKKVHEGMKVLDLINLIEGEIIKRG
                     ALPAFPCNISINEITAHYTSPPNDLTLINGGDLVKIDLGAHVDGFIADSAISVSVEGD
                     DPAGKMGPYKPQIQEEMIDTAKEALNTAISTIKPGVEVGKIGEEVEKTIHSRGLKPVS
                     NLTGHSMEQWILHSGLSIPNVKEKNHHKLEEGDVLAIEPFVTDGVGIVSDMKDAFIFR
                     FLRDRPVRMIQARRILEKIKTEYKILPFAQRWLEDKNQSSKSSIALRQLISSRAVYPY
                     HVLREKSGARVAQAEHTVIIESDGCLVITE"
     gene            2981..3400
                     /locus_tag="PKK85_01825"
     CDS             2981..3400
                     /locus_tag="PKK85_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24792.1"
                     /translation="MADVNIPQNIFWLIIVVIAVITIIIITMQWRRVREAQSNVEFLQ
                     KQAELKKIELVEKDLEAKRMMENVLPLPQDQQEKLAQIRGDTSKLMHKAGFMHSEINE
                     RVTRLEARTEYEKLQKLLSEIEKKEKELEKKGSKGGL"
     gene            3403..3972
                     /locus_tag="PKK85_01830"
     CDS             3403..3972
                     /locus_tag="PKK85_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24793.1"
                     /translation="MTALELFAILVLAGAIVVLLYYYLRDRSISIDTMRSDAVNLGET
                     VRSGASDLGGKVDRKGSMQDMGEKLHVSEVSEKVSGVGEKITISGVGERVSDVGKSLR
                     GKVKEVPISTDALSGKIDQFLEEKSDQLIKDWELATKKDIGEMEKKYSKVSRDLGDLE
                     RRFNEYRGHANKKFEHIEKRITVLEDKEE"
     gene            4100..4174
                     /locus_tag="PKK85_01835"
     tRNA            4100..4174
                     /locus_tag="PKK85_01835"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4135..4137,aa:Met,seq:cat)
     gene            4198..4485
                     /gene="rnpB"
                     /locus_tag="PKK85_01840"
     ncRNA           4198..4485
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKK85_01840"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
BASE COUNT         1603 a          922 c          941 g         1397 t
ORIGIN      
        1 accaggactg acgccggtga aaccatattc aaagcagctg tggatgcagg ggtcatcgaa
       61 accaaagcca tggacgatgt taaacctggt ttgggcatgt tagaaaaact ggccactgac
      121 aaaaagacca aagccatgaa ggtcattgat gacaggaaag caatgggctt gcctgttccc
      181 tacaaggcca gcagtgagaa agaagaccca ctagccaatg tttaagggat tcaaatccct
      241 taaaactatt ttttttcttg taaattcttg aataaattat ttatacctat ccaaattata
      301 ttaattttaa gtactgaatt ttattataca taaaataccc actttaccat acggccctaa
      361 tctgagtgtg ataaaatcaa atcagaaagt gataagagat catgtccgag gtgcggagct
      421 ttcaaggtgg aaaatgcccg tttctgcgta caatgcggta catcattgga taactatcct
      481 cagatcacct gtgtaatatg cagtacatgt ggtactgaaa atccatcata cgcctcattc
      541 tgtatggaat gtggagtcga tcttaaagtc actaatcgga aaacagaaga agatggtcac
      601 tttcccgata aaatggggtc acttgagtta actccagccg aatcactctt gatattggat
      661 cttgatatcg agggaaaaga ggtattcaaa ctggcaatgc tgaatctaat tgaccgccgc
      721 gtgatgaaga ttcgaacttt ccaagaagat aaaggatttc tggttaagga acctgtcctt
      781 ttccatgaag tacatcgggg agaaaatttt gaggccccac ttctacctta tgaagaaata
      841 tttcgcaaac cactctacag ccataatgaa atagagatct accgttacgt acagatgatc
      901 ttgaaaaata ttaaccccat tgtaacaaat tcatattcag aatacaaaga ggattacctc
      961 atcaattcac tgattaaagg gggttatata gaaaaaatag gaaaaaaagg ccttaattct
     1021 cccaaacctt ataagttaac ccaattagga gttgatatcc ggaaaaaaat tgttgatatt
     1081 ctggatgatg cggataacct gggggaatgg gtggacacag atccaaaacg ggctaaagca
     1141 tttttatcgg cattagggag tcacatattc ctcctcaact acgatttgga cacactccgt
     1201 tttttaacaa ataatttgac tggaattgaa tctagaactg ctaaatatta cagttactac
     1261 ctttctccca ttgatttttt taaagatttt aaatcagaac aagatataaa atatattttt
     1321 cgaataaatg ctctagaatc gtttgaattt tttgagacct ttgacccctt tgatgatatc
     1381 tttgattcat ttgaataggg tttattctat ttatattaat gtaactagaa ataactcaat
     1441 caaaaaataa cctatgaaga aaggataaat tagcaaaatt cctgatagtc acattttatc
     1501 tttgtccagt ccaaaatcaa ttaatttact cgctttttta acgagtttta aacgttcatt
     1561 ttaccattaa tcactttatc atccttcata aggtttccct ctcagatgat actaaccacc
     1621 accactgctg tcaatgtggg tatgaataat tagtgtaaac tcctttttca gatacataca
     1681 taggattatc atgatctctg ttaagcttat aagattatgc ataatttaaa aaatatatag
     1741 tgattatccc aataatcact ctgtaataac caaacagcca tctgattcta taatcacggt
     1801 gtgttcagct tgtgccactc tggccccgct tttttcgcgt agtacgtgat aaggataaac
     1861 tgcacgtgat gaaattaact ggcgtaatgc aatactggac ttgctggact gatttttatc
     1921 ttccaaccat ctttgggcaa aaggtaatat tttgtactcg gttttaattt tttccaggat
     1981 tctcctggcc tggatcattc taacaggtcg gtctctaagg aatctaaaaa tgaaggcgtc
     2041 tttcatatca cttactatcc ctactccatc ggttacgaat ggttctattg ctaagacatc
     2101 tccttcttca agtttatggt ggtttttttc cttcacattt ggtatagaaa gtccagaatg
     2161 cagtatccac tgttccatgc tgtgtccggt gaggttagac acaggtttta accccctgga
     2221 atggattgtt ttttcaactt cttcccctat ctttcccact tccactccag gtttgatggt
     2281 actaatagct gtgttcaatg cttcttttgc cgtatcaatc atttcctctt gaatttgggg
     2341 tttataaggg cccatttttc ctgctggatc atcaccttca acacttaccg aaatagcaga
     2401 atcagcaatg aatccatcaa catgagcgcc taaatcaatt ttaaccaaat ctccaccatt
     2461 tatcagggtc aaatcgttag gtggcgaggt gtaatgagca gtaatttcgt taatggaaat
     2521 attgcatgga aaagctggta aggcgccacg tttaatgatc tcaccttcaa ttaaatttat
     2581 cagatccagg accttcattc cttcatgcac cttctttaaa gcaagttccc ggacatcctt
     2641 aacaattttt cccgctttga tataattctc aatcatataa tcccttcaat aattaaaact
     2701 acttttataa tgtaaaaaac ttaataagcc gatcatattt tgtcgaacat catgggctta
     2761 aaatacaaga acatttaata tataaaaagt ccataatata tgtatctatc atcccctgta
     2821 taaaatatca acattttatg gaagattaga gtttaaacag acagagatga gttcagatga
     2881 ggatttattg agaacgtggg ctcaatttgt agaaatttcg tctacagtta atcactaaat
     2941 tttcattcaa atagtgatct tacaacaagg aggaatattt gtggctgatg tgaacattcc
     3001 gcagaatatc ttttggctta taatcgttgt aattgccgta ataaccataa taataattac
     3061 catgcaatgg agaagggtta gggaggctca aagtaacgtc gaatttttgc aaaaacaggc
     3121 tgaactcaag aaaattgagc tggtggagaa ggacctggag gctaaacgaa tgatggaaaa
     3181 cgtcttacca ttacctcaag atcaacagga aaaactagcc cagatcaggg gcgatacctc
     3241 caaattaatg cacaaagcag gattcatgca cagtgaaata aatgaaagag taactcgcct
     3301 agaggctaga accgagtatg agaaactcca aaaactctta agtgaaatag aaaaaaagga
     3361 aaaagaattg gagaaaaaag gtagcaaagg aggactgtga aaatgactgc actggagtta
     3421 tttgccatcc tagtcttagc cggggccatt gtagtactac tctactacta cttgagggac
     3481 cgtagcataa gcatagacac aatgcggagt gacgctgtga atttaggaga aactgtgcgc
     3541 agtggagcca gtgatctggg gggaaaggtc gataggaaag gctccatgca ggacatgggt
     3601 gaaaaacttc acgtatctga agtcagcgag aaggtctcag gagttggtga aaagataacc
     3661 atatctggag tgggagaaag agtttctgat gtgggaaaaa gtctaagagg taaagttaag
     3721 gaagtcccca ttagcaccga tgccttatcc gggaaaatag accaattcct ggaagaaaaa
     3781 agtgatcaac tcataaaaga ctgggaacta gccaccaaaa aggatattgg tgagatggaa
     3841 aagaaataca gcaaagtatc tagggatctt ggagacttgg agagacgttt caacgaatac
     3901 cgcgggcacg ccaacaagaa attcgaacac atcgaaaaaa gaatcaccgt attagaggat
     3961 aaagaagaat aacctggtga acctaaatac taaatctttt tttcatttat ttttggtatt
     4021 aacaagatta aatggctttt aataagtttt tattctgaaa ctaaaccttt atatactcta
     4081 aaatacaaca aaatggtaca gcggggtggg gtagtctggt gatcccgcgg ggctcataac
     4141 cccgagagcc ctagttcaaa tctaggcccc gctatttact tttattcctc aataatggca
     4201 agccgaaggg cagctaccgg tttttcgtaa actgaggaaa ctccacccat cgtacagacc
     4261 gtgatgtcta taggcatatg ctgagaagca tgactctgga gcagaaacga cacgtctcct
     4321 gttggatgaa catgaagccc aagcagatag acaacaggag gatcggtgaa acggccattc
     4381 cacgggatgc aagggcaaat actgctgatg aaaactgtat gaggcagagg tagcctgcaa
     4441 agatgaatgc tgccaggaac agaaggtggg ttactctcgg catgccatta actttttatt
     4501 catggaattg aattgtaact gatttttaaa ggatttatct gctttaaagt ttcatttcct
     4561 tgtataagaa aaaaataaag aaactatttt agtagatttc tggtatatca gtaaaactcc
     4621 cgaaatgatc tattcacaga ccaatcaata ttcctaccgt gaatgggtct ttatcaatta
     4681 gcctaataaa catgaattaa tccataccag aaaagttaca ccgttaccta ggctcccccc
     4741 atgttactag ttctgcagct atccctgcaa ttgttatcag aacacctgat cgtagatgac
     4801 aagcttccca ctatggtgaa cattgtcgga ttgaatatga aaccggcacc taaaatgaaa
     4861 gcc
//
LOCUS       DAOQVW010000049         4852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_19383_length_4852_cov_3.545549, whole genome shotgun sequence.
ACCESSION   DAOQVW010000049 DAOQVW010000000
VERSION     DAOQVW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19383_length_4852_cov_3.545549"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PKK85_01845"
     CDS             <1..893
                     /locus_tag="PKK85_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HNS24794.1"
                     /translation="YNRDLLANIDQDVIRSHAPQVVVECAHGIMAPITPLILEALDCE
                     TIMTGCQPTEKKFKFPEPSPESLHLISKLVMASGSDLGVALDNDRDRVVFIDEEGNIL
                     RDQTALAIFAKNALKENPGGKIVTSVVASMSLDEIVKDNGGTLIKTPVDLVLNGVLEE
                     GAVFGGDEPGLYVFPHFNLSFDAIYAALKMLEIICKSGKPLSKLVKEIPEYERHIFSI
                     ACEHAQKARVIESLCRYYKDRVELNMGDGFRADREDTVVVVRPSRFEPLVRVYLEAKS
                     PEKLMELQGEIKKLIKKFSR"
     gene            925..2424
                     /locus_tag="PKK85_01850"
     CDS             925..2424
                     /locus_tag="PKK85_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNS24795.1"
                     /translation="MVIKEDDPRVKVQKFLKDKHLIIASNRGPVEFKKDKKGNVELRR
                     GAGGLVSTLMPLMEAVKGTWVASAMSLVDAEVAAQYPENRVPITLDDPKFMIPYVVVD
                     RERYDYYYSVISNPLLWFVQHYMWNPPYTPEIDQNIHEAWEKGYLHVNRLFSRRIVEE
                     VERSEDEPVVMLQDYHLYLCPTLIREELDDIFLSQFIHIPWPQADYFSILPSYMEESI
                     IRGLLSNDLLGFHIPKYVYNFLSTCERYADAVDYQRQQVQFEGRVVHVKSYPISVDDE
                     GIKSSAASEEVREKEKLIKELKGDNILIYRTDRADLSKNIVRGFRAYDLFLEEHPEFR
                     GKVKFLTTGKPTRQQIKEYRDYHTEIQRIIGEINSKHLKDGWKPIEHIFRADYSLVLA
                     AFKNYDCLIVNPIADGMNIVPKEASVINEENGVLILSDKAGCYEELKDYSLNVNPFDV
                     SETAEAFYTALTMDENEKKQRMQGLKKKISERNIFHWITDQFQDIENLF"
     gene            complement(2446..3216)
                     /locus_tag="PKK85_01855"
     CDS             complement(2446..3216)
                     /locus_tag="PKK85_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HNS24796.1"
                     /translation="MDLAGFIDTHIHTSPDTRPRITSDVEAGIQARKAGMEAIVIKSH
                     LEPTSARAQLASELADFKVIGGVTLNLSVGGLNSHAVEASALHGGRIVWLPTIHHQEV
                     DLEEEKLEEVLVAVRDYDLALGTGHLSPVEILQVLDHAKSLGVKRLLVNHPLTQVVNA
                     SLEEQKEMSRRAYLEHCYVATRPLHDGLGLEKIAQAIQEVGPDRCIMATDLGQIHNPT
                     PVSGLRIFAEEMIGQGISREEVERMCRDNPAKLIFPGP"
     gene            complement(3221..3763)
                     /locus_tag="PKK85_01860"
     CDS             complement(3221..3763)
                     /locus_tag="PKK85_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNS24797.1"
                     /translation="MEFKLKTPLSGGETRKLRVKDTVHLSGTIYTARDSAHKRMVEEG
                     APMDLEGAVIFHAGPIIQEEDGQYRMVAVGPTTSTRMNPYQAQVLDGGVKAVIGKGGM
                     DRTTMEALKRNGAVFLSAVGGCAALYVQAVKRIEHVFWLDLGVPEAIWELEVENFGPL
                     IVTMDSTGRDLYQEARQGGE"
     gene            3859..>4852
                     /locus_tag="PKK85_01865"
     CDS             3859..>4852
                     /locus_tag="PKK85_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HNS24798.1"
                     /translation="MSAVDNLMGLTKYLVVLPESNISILSMVFLGFLTGTMASYLEPG
                     INLFDSVVYGGSAGFLIFGLMSIMAGALTQPWVNSLEGRHMKMKQSLFLALVTMLVVA
                     LVYLVGSLISTFTFNSYTINALIYGCALAFALRTLVIWGTSNISFLQSLPVAATQPVL
                     ILSMVLVLVSLTSLETNFGPFSILALVPKAIIAGAILILAIYSFVLVIESPIKRNLGV
                     GGLELLSLFIAHITEGSDALENVFQDMGEPIDTLVGVVSFKGEEKVKGILISPCVHPG
                     PVGSIGGGNMPTELAQHMDCFTMVCHGPSTHDFNPVSAREIRKIEKVVRDALDEM"
BASE COUNT         1160 a         1225 c         1298 g         1169 t
ORIGIN      
        1 tctacaaccg ggatctgctg gctaacattg accaggacgt gatcagatcc cacgcccccc
       61 aggtggtggt ggaatgtgcc catggtataa tggcccccat cacccccctt atactggaag
      121 ccctggattg tgaaaccatc atgaccgggt gccagcccac cgaaaaaaag ttcaaattcc
      181 cggagccaag tccagaaagc cttcacctga tatctaagct ggtaatggcc agtggctcag
      241 atctgggtgt tgccctggac aatgaccggg accgggtggt tttcattgat gaagagggaa
      301 acatcttaag ggaccaaact gccctggcaa tatttgccaa gaacgccctg aaagaaaacc
      361 ctggaggaaa aatcgttaca tcagtggtgg cctccatgtc ccttgatgag atagttaaag
      421 ataatggggg aaccctcatc aagaccccgg tggatttagt tttaaatgga gtcctggagg
      481 agggagctgt ttttggtgga gatgagccag gtttgtacgt tttcccccac ttcaatttat
      541 ccttcgatgc catttacgcg gcgctgaaaa tgttggaaat tatctgtaaa agtggcaaac
      601 ccttatccaa gctggtgaag gagatacccg aatacgagag gcacatattt tccatagcct
      661 gtgaacacgc ccagaaagcc agggttatag agagtttatg cagatattac aaggataggg
      721 tggagttgaa tatgggagac gggttccggg cagataggga ggatactgta gtagtggtaa
      781 ggccctcccg attcgagccc ctggtacgtg tttatctgga ggccaagtca ccggagaagt
      841 taatggagct gcagggtgaa attaagaagc tcatcaagaa gttttccaga taataggaat
      901 cataaaaaaa agaggggttg ggagatggtt attaaagaag atgacccccg ggttaaggtt
      961 cagaaattcc tgaaagacaa gcacctgata atagcctcca accggggacc agtggaattt
     1021 aagaaggaca aaaagggcaa cgtagaactt agaaggggtg ctggaggact ggtttcaacg
     1081 ttaatgccct tgatggaggc ggttaaaggt acctgggtgg ccagtgccat gtccctggtg
     1141 gatgccgagg tagcagcaca gtacccggaa aatcgggtcc ccataaccct ggatgatcct
     1201 aaattcatga tcccctatgt ggtggtggat cgggagaggt acgattacta ctacagtgta
     1261 atcagcaacc ccctcctctg gtttgtgcag cattacatgt ggaatccgcc ctacacccct
     1321 gagatcgatc agaatatcca cgaggcctgg gagaagggtt atctccatgt gaatcgtctt
     1381 ttttcacgga ggatagtgga ggaagtggag agatccgagg atgagccagt ggtgatgctc
     1441 caggattatc atctctacct gtgccccacc ttgatccgtg aagaactgga tgatatcttc
     1501 ctaagccagt ttatccacat cccctggccc caggcagact atttcagtat actcccctcc
     1561 tacatggagg aatccataat cagggggctt ctctccaatg acctcctcgg atttcacata
     1621 ccaaaatacg tgtacaactt ccttagtacc tgcgaaaggt acgctgatgc agttgactac
     1681 cagaggcagc aggtccaatt tgagggtagg gtggtgcacg tgaaaagtta tcccatctca
     1741 gtggatgatg aagggataaa atcctctgct gcctcagaag aggttaggga aaaggagaaa
     1801 ttgataaaag agttgaaggg ggataacatc cttatttatc gcaccgaccg ggcggatctg
     1861 agtaaaaata ttgtgagggg cttccgggcc tacgatctct tcctggagga gcacccggaa
     1921 ttcaggggta aagtcaaatt cctaaccacc ggcaaaccca cccggcagca gataaaggag
     1981 taccgggatt atcacacgga gatccagcga attatagggg aaattaactc caaacacctt
     2041 aaagacggat ggaaacccat tgagcacata ttcagggctg attactccct ggtcctggcg
     2101 gcctttaaga actacgattg cctgattgtc aaccccattg ctgatggtat gaacatcgta
     2161 cccaaggagg cctcggtgat caacgaagaa aatggggtgc tcatcctgtc tgataaggcc
     2221 gggtgctacg aggagttaaa ggattattcc ctgaatgtga accccttcga tgtatctgaa
     2281 actgcagagg ccttctacac agcccttacc atggatgaga atgagaagaa acagcgcatg
     2341 cagggcttga aaaaaaagat aagtgaaagg aacattttcc actggataac tgaccaattc
     2401 caggacatcg agaatttatt ctaatcctca acttgattcc tgtttttagg ggcctggaaa
     2461 gatgagtttg gctggattat cacggcacat cctttccacc tcttccctgg aaattccctg
     2521 gcctatcatc tcctcggcaa agatccggag gcccgaaact ggggtggggt tgtggatctg
     2581 gcccaggtca gtggccatga tacaccgatc cggcccaacc tcctggatgg cttgggctat
     2641 tttctccagg cccagaccat catggagggg cctggtggcc acgtagcagt gctccaggta
     2701 ggccctcctg gacatctcct tctgctcctc cagggatgca ttcaccacct gggtaagggg
     2761 atggttaacc agcaatcgct tcacccccag gctcttggca tggtccagaa cctggagtat
     2821 ctccacaggc gagaggtggc ctgttcccag ggccaggtcg tagtcccgca ccgctaccag
     2881 cacttcctcc agtttttctt cctccaagtc cacctcttgg tggtggatgg tggggagcca
     2941 gactatccgg ccaccgtgga gggctgaggc ctccactgca tgggagttaa gacccccaac
     3001 actgaggtta agggtcactc cccctataac cttaaagtca gcaagttcag aggctagctg
     3061 ggcccgggcg gaggtgggtt ccaggtgtga tttgatgacg atggcctcca tccctgcctt
     3121 cctggcctgg atgcctgcct ccacgtcgct ggtgatccgg ggccgggtat caggggaggt
     3181 gtggatgtgg gtgtctataa atccggctaa atccacagct tcactcccct ccctggcgtg
     3241 cctcctggta gaggtctctg cctgttgaat ccatggtgac gattaagggg ccgaagtttt
     3301 ccacttccaa ctcccagatt gcctctggaa cccccaggtc cagccagaaa acatgttcaa
     3361 tcctcttcac ggcctggacg tagagggcgg cgcagccccc caccgctgat aggaacacgg
     3421 ccccgtttct tttaagtgcc tccattgtgg tgcggtccat gccaccctta cctataactg
     3481 ccttaacacc tccatccagg acttgggctt ggtaggggtt catcctggtg ctggtggtgg
     3541 gtcccaccgc caccatccgg tactgtccat cctcttcttg gattatgggg ccggcgtgga
     3601 atatcaccgc accctccaga tccatggggg ccccttcctc caccatccgc ttgtgggcgc
     3661 tgtcccgggc tgtgtaaatg gttccggaaa ggtgtactgt gtctttgact cgtaattttc
     3721 tggtttcccc ccctgagagg ggtgttttga gtttaaactc catattttgt ctcctggtct
     3781 atctttttat agggtgaagg gtaatggtta tattgctatc tttaccattc atcagcatta
     3841 aaagtttaga ggtaggttat gtcggcagtt gacaatctta tggggctaac caagtacctt
     3901 gtggttctgc ccgagagcaa tatatccatt ttgagcatgg tattcctagg cttcctcacc
     3961 ggaaccatgg cctcctatct agaacccggg atcaacctct tcgatagtgt agtgtacggt
     4021 ggttcggctg ggtttctcat cttcggtttg atgtccatca tggccggggc cctgacccag
     4081 ccctgggtta acagcctgga gggccggcac atgaagatga agcagtccct gttcctggcc
     4141 ctggtcacca tgctggtggt ggccctggtg tacctggtgg ggagcctgat atccaccttc
     4201 acatttaaca gttacaccat aaacgccctg atatacggct gtgccctggc cttcgccctg
     4261 aggaccctgg ttatctgggg gaccagtaac atcagcttcc tccagtcatt accagttgcc
     4321 gccacccagc cggtgctcat cctgagcatg gtcctggtgt tggtgtccct aaccagcctg
     4381 gaaaccaact tcggcccgtt cagtatactg gcactggttc ctaaggccat aattgcaggt
     4441 gccattttga tactggccat atattcattt gtactggtaa ttgaatcccc cataaaacgt
     4501 aaccttgggg tggggggcct ggagttgctg agcctcttca tcgcccacat aactgaggga
     4561 tctgatgccc tggaaaacgt tttccaggac atgggcgaac ccatcgacac cctggtaggg
     4621 gtggtgagct tcaagggtga agaaaaagtg aagggcatcc taatatcccc ctgcgtccat
     4681 cctgggccgg tggggagcat aggtgggggt aacatgccca ctgaactggc ccagcatatg
     4741 gattgcttta ccatggtctg ccatggtcca tccacccatg atttcaaccc agtatcggcc
     4801 cgggagatca ggaaaataga aaaggtggtg agggatgctc tggatgagat gg
//
LOCUS       DAOQVW010000050         4611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_20805_length_4611_cov_3.766023, whole genome shotgun sequence.
ACCESSION   DAOQVW010000050 DAOQVW010000000
VERSION     DAOQVW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20805_length_4611_cov_3.766023"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1013)
                     /locus_tag="PKK85_01870"
     CDS             complement(57..1013)
                     /locus_tag="PKK85_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036094 - small molecule binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3H domain-containing protein"
                     /protein_id="HNS24799.1"
                     /translation="MRKPYVILIGSASGIGKSTISSEIAKELGIKHLIETDFIREIVR
                     GIIGPEYAPALHKSSYDAYVTLKDKNRYESNGEALISAGFEEHASFVIPAIEKVIKRA
                     VDDFDDVVIEGVHLVPGLLNIKKFQEDAAIHFFILTADEETHKERFVKRAMKIKRGGK
                     HLEYFKENRIINNYLVKQARDHHVPVINNLDLDNTKKRMLTIIRENCTELVLQHTVDQ
                     LEEVTHIVLNKYGGRIVDVSYFLPGFGEPLKRKVNVYDPVEAERFITLIKKNPKRRKD
                     LQGLYELSNNIHSNQICAPDEESLQNIIEELEKRGLVYHPEP"
     gene            complement(1107..1457)
                     /locus_tag="PKK85_01875"
     CDS             complement(1107..1457)
                     /locus_tag="PKK85_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNS24800.1"
                     /translation="MIERILKDLGRINGVNGSLVVGKDGLIIESEVPSDIDSELVAAM
                     ASAVFGTAERSAEEMKHDPLQQVMIEGNKGKTLMIDSGEGILVVITDIDINLGLIRIE
                     MRRSAERVVDFLGS"
     gene            1576..2631
                     /locus_tag="PKK85_01880"
     CDS             1576..2631
                     /locus_tag="PKK85_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HNS24801.1"
                     /translation="MNFVSSVPELQELNPYIVIGCGGGGEKFSNFQGVKALGFIDDNP
                     EKQGQEFCNKIIASDLEALASDTGAKSVAIMLPIGAEGSALKYAVQAIDQGLNVVTSF
                     RSLSLQRNEALLKLAKSRGVKIMEISPRLDVIHDIFGTSPPSCTETLPKLTYKPSVPV
                     IFVGGTSQECGKRTTTRILGKTAKENGMNPGIISTDEMGLEQPVDLNFRAGSLSVMDV
                     ASAVMGAMRKIEEQKNPDILLVEGQSSLTERGNPHPRGLSAAILVGAQPDATLVCHRP
                     NHPYRHPVGIRQEIMAIEAMEPTVVVGISLNLRNVEDKGVVSEYETRYGLPTVDVYRE
                     GASRLLDAIINETGEKT"
     gene            2628..2984
                     /gene="sepF"
                     /locus_tag="PKK85_01885"
     CDS             2628..2984
                     /gene="sepF"
                     /locus_tag="PKK85_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNS24802.1"
                     /translation="MKDVMDILKKNLGMEENEEEDEENETIIVPEHSFYEIILMKTNN
                     LDDFDYALSQVVDAKNPIIMDMTLLEKDYPEDFKIAAEKLKSFRDTKGGEAILLCQNG
                     KSIIIVTPPEIKLIRK"
     gene            3031..4323
                     /locus_tag="PKK85_01890"
     CDS             3031..4323
                     /locus_tag="PKK85_01890"
                     /inference="COORDINATES: protein motif:HMM:NF021502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2226 domain-containing protein"
                     /protein_id="HNS24803.1"
                     /translation="MDLPITKPTMMAYADQLDFSQLLEELSQTKKNGFVRVTSGSEEG
                     YLLFKDGEQVAASYDNLSKLEAVDKIKLSMEDYKTLVEVFDVRPAQIPYLMDLNKAHL
                     LEKVQDIDEVLEELKKEPAEDETSEEAKETLPGTGSEAELPVTPDPDKTWEAPESSGE
                     PSVETLNKLENTVTAEPSPEIPTPEVAVDGTVTDVEKEIRDESPRPSSPNTGQESAVK
                     TSPETSAEAFSQVMDKISSTPTEVPELKTAEEETRLEENEISHIPNDDDPGIPPLDDA
                     NTDETAVDGSPETENEEPLDRMELLKKYGIKDINEEEVEKVLGSYTGGYLSDNDVEKV
                     ELTLMNRVKKSIMGLPKVKGTEVMIFLDNAGNDLKGNINVIMEYQSQGFFSRIVGESK
                     EINKLKRQVIGICNMEIRKIFREYPEVVDKFEVNVEIS"
     gene            4335..>4611
                     /locus_tag="PKK85_01895"
     CDS             4335..>4611
                     /locus_tag="PKK85_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNS24804.1"
                     /translation="MTRVISVASGKGGVGKTTITANLGVALATYGEEVIVLDADVAMA
                     NLELILGMEGKSITLQDVLSGEATIDEAIYEGPGGVKIVPAGISLEGL"
BASE COUNT         1381 a          999 c         1043 g         1188 t
ORIGIN      
        1 ctcctgataa atccgtggga tgactggatg gttgacaagt gaaccttgac tcgggttcat
       61 ggttctggat ggtagaccag ccccctcttt tcaagttcct ctatgatgtt ctggaggctt
      121 tcttcatcag gggcacagat ctggttactg tgaatattat ttgacagttc gtaaagtccc
      181 tgcagatctt tacgcctttt cggattcttc tttatgaggg ttatgaaacg ctccgcctcc
      241 acggggtcgt acacattgac cttccgtttc aggggctctc caaagcctgg taggaagtag
      301 gaaacatcca cgatccgacc cccatacttg ttaagaacta tatgggtaac ctcttccaac
      361 tggtccacgg tgtgctgcag caccagttca gtgcagttct cacggatgat ggtgagcatc
      421 cttttcttag tgttatccag atccaagttg ttgatcaccg ggacatgatg gtcacgggcc
      481 tgcttcacca aataattgtt gattatgcgg ttttctttaa aatattccag atgtttgccc
      541 cctcttttta tcttcatagc ccttttaaca aacctttcct tgtgtgtttc ctcatcggca
      601 gttaagataa aaaaatgtat agcggcatct tcctggaatt tctttatatt aagcagccca
      661 ggaaccaggt gcaccccctc aatcaccaca tcatcaaaat catcaaccgc ccttttaata
      721 accttttcaa tggcgggaat tacaaaagaa gcatgttctt caaagccagc actgatgagt
      781 gcctcaccat tactctcata acggttcttg tccttcaggg taacgtaggc atcatatgag
      841 gatttatgaa gggcaggggc gtattcaggg ccaatgattc ccctcacaat ctctcttata
      901 aaatcggttt caatcaggtg tttaatacct aattctttgg ctatttcaga ggatatggtt
      961 gattttccaa tccctgatgc acttccaatt aggattacat atggttttct caagacagcc
     1021 tcctccagag ttcatggatg tattatcaat tctatgaaga gtagttggaa agaggttagg
     1081 ggtaccatgc cagataaaga aaggggttag gagcctaaaa agtccactac ccgttcagca
     1141 ctgcgccgca tttctattct aataagacct aaattgatat caatatcagt aataactacc
     1201 agtatacctt caccagaatc aatcataaga gtttttccct tatttccctc aatcattact
     1261 tgttgaagtg ggtcgtgctt catctcttct gcagaacgct cagcagtacc gaacactgcg
     1321 gatgccattg ctgctaccag ttccgagtca atatctgatg gtacttcact ttcaataatg
     1381 agaccatctt taccaaccac tagtgatccg tttaccccgt tgatccggcc taagtcttta
     1441 agtatccttt ctatcatact atgcctccac tacaaattga gtaggctaac caaaatatat
     1501 atcttgcttt agtatttatt aaataaatag gtaatgtaat aattcacatg ttcaccacat
     1561 atgaggagtg attttttgaa ttttgtatct tctgttccgg aactacagga gctcaacccg
     1621 tacatagtaa ttggatgtgg aggtggcggc gaaaaatttt caaacttcca gggtgtgaag
     1681 gccctgggct ttattgatga caacccagag aaacagggtc aggaattttg taataagata
     1741 atagcatcag atctagaggc cctggccagt gatacaggtg ctaaaagtgt ggctatcatg
     1801 ctccccatag gtgccgaggg atcagctttg aaatacgcgg tgcaggccat tgaccaggga
     1861 cttaatgtgg tcacttcctt ccggtcacta tccctgcaac gtaacgaggc ccttctgaag
     1921 ctcgccaaaa gccgtggcgt taagataatg gagataagcc ccagattgga tgtgatccat
     1981 gatatctttg gaacctcccc tcccagttgc acggaaacac tccctaaatt gacttataaa
     2041 cctagcgttc cagttatttt tgtgggaggc acctcccagg aatgtggtaa acgaaccacc
     2101 acccgtatac ttggtaaaac tgcaaaagag aatggaatga atccgggaat catatcgacc
     2161 gatgaaatgg gcttggaaca gcccgtggat ctcaacttcc gggctggcag tttatcagtc
     2221 atggacgtgg catcagcggt gatgggtgcc atgagaaaaa ttgaagaaca aaaaaatcct
     2281 gatatcctat tggtggaggg tcagtccagt ttaacagaaa ggggcaaccc ccatccccgg
     2341 gggctttccg ccgccatact ggtgggggcc cagccagatg ccaccctggt atgccaccgg
     2401 cccaaccatc cgtaccggca tccggtgggg ataagacagg agatcatggc catagaggct
     2461 atggaaccca ctgttgtagt gggaatttct cttaacttgc ggaatgttga ggataaagga
     2521 gttgtttctg aatatgaaac ccgatatggc cttcctaccg ttgatgttta cagggaggga
     2581 gcatcccgtt tattagatgc gataattaac gaaacagggg aaaaaacatg aaggacgtaa
     2641 tggacatttt gaagaaaaac ctgggcatgg aagaaaatga agaagaagat gaggagaatg
     2701 agacaataat tgtccctgaa cactctttct atgagattat tctcatgaaa accaacaatc
     2761 tggatgattt tgactacgct ctttcccaag tcgtggatgc caagaatccc ataatcatgg
     2821 acatgaccct actggaaaag gattatccag aagattttaa gatagctgct gaaaaattga
     2881 agtcatttag agatactaag ggtggagagg ccatactcct gtgccagaat ggtaagagca
     2941 tcatcatcgt cacccctcct gaaattaaac taatccgaaa gtaaagtaca actcctaaaa
     3001 tcccgtacct gaataatgga gagtggaaca atggacctgc ccataaccaa acctactatg
     3061 atggcctatg ctgaccaact ggatttttcc cagcttttag aggaactatc acaaactaaa
     3121 aagaatggtt ttgtaagggt aacatccgga tcagaagagg gatatctgct atttaaggac
     3181 ggagaacagg ttgctgcttc ttatgataac ctgtccaaat tagaggcggt agacaaaata
     3241 aaactatcca tggaagacta caaaaccctg gtggaggttt tcgatgttcg acccgcccag
     3301 attccatact tgatggatct caacaaggcc cacctcctgg aaaaagttca ggatattgac
     3361 gaagtactgg aagagcttaa aaaagaacca gcagaagatg aaacctcaga agaagccaag
     3421 gaaactctcc caggaactgg atctgaagca gaacttcccg tcacccctga tcctgataaa
     3481 acatgggaag ctcctgaatc atcaggggaa ccatcagttg aaactctgaa taagttagaa
     3541 aatactgtga cagcagaacc atcccccgag atccctaccc cggaggtggc agtggatgga
     3601 acagttaccg atgtggaaaa agaaataaga gacgaatctc ctaggccttc ttccccgaac
     3661 actggccagg aaagcgctgt aaaaacgtca ccagaaacat ctgctgaagc tttctctcag
     3721 gtgatggata aaatttcatc tacacccacc gaagtccctg aactcaaaac agcagaggaa
     3781 gaaacacgcc tggaagaaaa tgagatctcc cacatcccaa atgatgatga tcctggaatc
     3841 cctcctttag atgatgctaa cactgatgaa acagcagtgg atggatctcc agaaacagaa
     3901 aatgaggaac ccctggatcg tatggaacta ctcaaaaaat atggaatcaa ggatattaac
     3961 gaagaggaag tggaaaaagt cctcggatcc tacactggtg gttatctctc tgataacgat
     4021 gtggaaaagg tggaacttac actgatgaac cgggttaaaa agtctataat gggattgcca
     4081 aaggttaagg gcacagaggt aatgatcttc ctggataatg ccggcaacga ccttaagggt
     4141 aacataaacg tgatcatgga ataccagtcc cagggatttt tctcccgaat cgtaggggaa
     4201 tcgaaagaaa ttaataaatt aaaaagacaa gtaataggta tttgtaacat ggagatcaga
     4261 aaaatattcc gggaatatcc ggaagtggtg gataaatttg aagttaacgt agagattagc
     4321 taaggaggta tatgatgaca agagttataa gtgtggcttc gggtaagggt ggagttggaa
     4381 aaactactat caccgctaat cttggtgtgg cattggctac ctatggggaa gaggtcattg
     4441 tcctggacgc tgacgtggcc atggccaacc tggaactcat cctagggatg gagggaaaat
     4501 ccataaccct tcaggatgtg ttatctggtg aagcaaccat tgacgaagct atatatgaag
     4561 gtccgggtgg cgtgaagatc gttccagcag gaatatcatt agaaggactt c
//
LOCUS       DAOQVW010000051         4499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_21494_length_4499_cov_3.240999, whole genome shotgun sequence.
ACCESSION   DAOQVW010000051 DAOQVW010000000
VERSION     DAOQVW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21494_length_4499_cov_3.240999"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PKK85_01900"
     CDS             <1..258
                     /locus_tag="PKK85_01900"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNS24805.1"
                     /translation="ASSLMLKAMANRRLDQPSELEIGITESLNQSGIGSLGLGGTITA
                     LGSLVLVGPQRASGVRIVCMRPCCSVEPRRSWVYLPPDLLE"
     gene            264..1115
                     /locus_tag="PKK85_01905"
     CDS             264..1115
                     /locus_tag="PKK85_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HNS24806.1"
                     /translation="MAIGRESVENLIKIAKPKWKTSISQVSPNQITTRGFLQEDLIEN
                     MSFSEMVYLLLKGEIPKKNQSKMLQSVLVSLCDHGVTPPSTQVARLMASTGSSMSSCV
                     AGGLLSFGKHHAGALKLSMKILQDALKGVEIDGTDLESDDQVQRAAHLVVEQHQSKGE
                     KIPGFGHRFHQQDPRPPKLIDMAIKYNCFGIHTELALKIQEILFETKGIRINIDGANA
                     GILSDLGFGWELGTGIFMIGRIPALVAHVNEEKVRETPFRKLFEIEEIYYDGPESKKS
                     DDILMND"
     gene            1229..1543
                     /locus_tag="PKK85_01910"
     CDS             1229..1543
                     /locus_tag="PKK85_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit H"
                     /protein_id="HNS24807.1"
                     /translation="MTTISEAITTIKKAENASDKLIEDAKVKSSEIIEESKSKTIETI
                     EAAREQANSEAEKILFEAETSAKKEAYHINRETGEKVEQTKNKATGMVDEAAEVIVKS
                     IL"
     gene            1554..3557
                     /locus_tag="PKK85_01915"
     CDS             1554..3557
                     /locus_tag="PKK85_01915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HNS24808.1"
                     /translation="MFKPARMKKLKIITLDKYADSAVDSLHEAGLVQIEDISERIQQD
                     AEWRQILKPSGASPFTGKISSLLMKTTGALDFLKSMERKEKGILPMVKGFVNPPPIEK
                     MNVEILGVQDLIDDTENILEDVGSKTRPLEEKLNQLDIRKSEVENALKVSQNLINFDI
                     DLSLLGESDYVSYFAGKISSESYDTFKGKDLPDEIMVFDQDTELKNYKILVVLTLKEH
                     ADEVLSQLRKLEFERLEVSGLSGKPSDIIEQSKNEIESIKQEKESVLDDLAMISGEWV
                     GKLQCLKEQLEIQKQRSEIFSSFGETKNTVMFEGWVTEKKLNDTLSTIETSTEGHSIV
                     DVSDPDVNTDKVPVHLDNPRFAKPYEMFVHMYSPPDYREIDPTILMAIVFPFFFGFCL
                     TETGYGIVDALIGYIIYRGMGRNSKTWANLGLIMVACGVWAIILGLVTNSLIGDFIPR
                     WIFGDPSAMLPTTIQSINSFVHPENILIIALIVGVIHINMGLVFGAYNNIIRGEVREA
                     LGAQIVWFVLELGVVLLAIGYLFGMGLLLYSGVGILVLSILMLIYFNGLFGLMDMSGF
                     LGNVLSYARLLALCLSTGGIATTVNILTGIVADMIPVIGIVLAPIVFIGGQIANGAFQ
                     TLGAFINSLRLHYVEFFAQFYMGGSQKFRAFRAKRKYTDLGGK"
     gene            3559..4038
                     /locus_tag="PKK85_01920"
     CDS             3559..4038
                     /locus_tag="PKK85_01920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032187.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNS24809.1"
                     /translation="MVEIALGTALAAIGAGMAVGFAGLGSGLGQGIAAAGSVGAVAED
                     EDMFARGIIFTALPETQAIYGFLIAILIMVFTIMAGKVLPASLGLVAIGAGAAIGFAG
                     LGSGMGQGITASSAVGAVVENEDMFARGIIFTALSETQAIYGFLIAILLMVFGGILG"
     gene            4044..>4499
                     /locus_tag="PKK85_01925"
     CDS             4044..>4499
                     /locus_tag="PKK85_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNS24810.1"
                     /translation="MSGADKIVSSIMSDAQIKAESIIEEAENKSISILKEGEGQAVLE
                     KERILENAEKQSQMRYQQIISEAKMNSRRMELEAREKIIEEAFQKAEEKLKRIASSDA
                     PEYKASLEKVITEAGVEIGGGNLVVLVKKEDETKISSSIPAIEKSISEES"
BASE COUNT         1400 a          843 c         1051 g         1205 t
ORIGIN      
        1 gcttcatctt taatgctcaa ggcaatggca aaccggaggc ttgaccagcc atcagagtta
       61 gaaataggta taactgaatc cctcaaccag tccggaattg gctccctggg attgggtgga
      121 accataactg ccttgggaag cctggtactt gttggtccgc aacgggccag tggggttcga
      181 atagtatgta tgcggccctg ctgttctgtg gagccccgca ggtcatgggt gtatttacca
      241 ccggatctat tggagtgaat ataatggcaa ttggaagaga atctgtggaa aatcttatta
      301 aaatcgctaa gccgaagtgg aagacttcca tttcccaggt gtcacccaat caaatcacga
      361 cccggggatt tctccaggaa gatctcatag aaaatatgtc cttctctgag atggtttatc
      421 tgcttttgaa gggtgaaata ccaaagaaaa atcagtccaa aatgttgcag tccgttctgg
      481 tttctttatg tgatcatgga gtcactcccc ccagcaccca ggtagcccga ttgatggctt
      541 ctactggatc ttccatgagc tcctgtgtgg caggtgggct tttatccttc ggcaaacatc
      601 atgctggggc tttaaaactt tccatgaaaa ttttacagga tgctttgaag ggtgtggaga
      661 tagatgggac tgatttagaa agtgatgacc aggtgcaaag ggcagcccac ctggtagtgg
      721 aacaacacca gagtaagggt gaaaaaatcc caggatttgg acaccgattc caccaacaag
      781 atcccagacc accaaaactc attgatatgg ccattaagta caactgtttt ggaattcaca
      841 ctgagttagc cctaaaaatt caggaaatac tcttcgaaac caagggcatt cgtataaata
      901 ttgatggagc caatgccgga atactttcag atttaggttt tggatgggag ttaggaactg
      961 gtatattcat gattggaaga attccagcac tggttgccca tgttaatgag gaaaaagtca
     1021 gagaaactcc ttttagaaag ctatttgaga ttgaagagat ttattatgat ggtccggaaa
     1081 gcaaaaaatc agatgacatt ttaatgaatg attaaaagta ttttttgtgc tagaaaattc
     1141 ctgttttttc ccggaaaaat aaatagcaaa tcataaatat aactcaaaat agaattccgt
     1201 ttatcatagt cataataggg tgatttaaat gacaacaata tctgaagcta tcactaccat
     1261 taaaaaggcc gaaaatgcct ctgataaact catagaagat gctaaagtta aatcgtcaga
     1321 gattatcgaa gaatccaaat caaagaccat tgaaactatt gaagcagccc gagaacaggc
     1381 aaacagtgaa gcagagaaaa ttttgtttga agccgaaacc agcgccaaaa aggaagcgta
     1441 ccacataaac cgtgaaactg gtgaaaaagt agagcagact aaaaataaag ctaccggtat
     1501 ggttgacgaa gctgcagaag tcattgttaa aagtatattg taacgtgagt accatgttca
     1561 agccggcaag gatgaaaaag ctcaagataa tcaccttaga caagtacgct gattctgcag
     1621 tggattcact tcacgaagcg ggcttggtcc aaatagagga catatctgag cgaatacaac
     1681 aggatgcaga gtggaggcaa attctcaaac cttcgggagc ctctcctttc acagggaaaa
     1741 tatcttcact tctgatgaag accaccggag ccttggattt cctgaaatcc atggaaagaa
     1801 aggaaaaagg gattttaccc atggttaaag gttttgttaa tccaccaccc atcgagaaga
     1861 tgaatgtaga gatcttgggg gttcaggatc tcattgacga taccgaaaac atcctggagg
     1921 atgtgggttc caagaccaga cccctcgagg aaaagttaaa ccaacttgac atcagaaagt
     1981 cagaagtgga aaatgcacta aaagtttccc aaaaccttat aaattttgat atcgatctct
     2041 cacttctggg agaatctgat tatgtgtctt attttgccgg gaaaatatct tcagaatcct
     2101 atgatacctt taaaggaaaa gatctgcccg atgaaataat ggtttttgac caggatactg
     2161 aactgaaaaa ctacaaaatt ttagttgtcc tgaccttgaa ggaacatgcc gacgaagttt
     2221 taagtcaact gcgtaagctg gagtttgaaa gattagaagt ttcaggctta tcaggtaaac
     2281 ccagtgatat tatagaacaa tcaaaaaatg agatagaatc cataaaacag gaaaaagaat
     2341 ctgttttaga cgatctggca atgataagtg gggaatgggt tggtaaactt caatgtctca
     2401 aggaacaatt agaaatacag aaacagagaa gtgaaatctt ctcatccttt ggtgaaacca
     2461 aaaatactgt catgtttgaa ggttgggtga ctgagaaaaa attaaatgat accctatcaa
     2521 ctatcgaaac ttcaactgaa ggtcattcca ttgttgatgt ttctgatccg gatgtaaaca
     2581 ctgataaagt tccagtacat cttgataacc cccgttttgc caaaccctat gagatgtttg
     2641 tgcacatgta ttctcctcca gattaccggg agattgaccc caccatcctc atggccattg
     2701 tgttcccctt tttcttcggt ttctgtttaa ctgaaactgg ttacgggata gtggatgccc
     2761 ttataggtta tattatctac cgtggcatgg ggagaaacag caaaacttgg gctaatctgg
     2821 gcctgattat ggtggcttgt ggtgtgtggg ccattatcct gggacttgta accaacagtt
     2881 taattggaga cttcataccc cggtggatat ttggtgatcc aagtgccatg cttcccacca
     2941 ccatccaatc cataaactcc ttcgtacacc cggagaacat cctcataatc gcgttgattg
     3001 tgggtgtgat ccacattaac atgggcttgg tatttggtgc ctacaacaac ataatccgag
     3061 gcgaagttcg ggaagcacta ggagctcaga ttgtgtggtt cgtccttgaa ctcggcgtgg
     3121 tgttactggc cataggatac ctcttcggaa tgggattatt actttattct ggagtaggaa
     3181 tcctggtttt aagcatactc atgctgattt atttcaacgg tctttttgga ctgatggata
     3241 tgtcaggttt cctgggaaac gtcctttcat atgccaggct cctggcatta tgtctttcca
     3301 cgggaggtat agccaccacc gttaacattc taaccggaat tgtggcggat atgattcccg
     3361 ttattggaat agtactggca ccaattgtat ttattggagg gcaaattgca aacggtgcct
     3421 tccagacgct aggtgcattt ataaattcac tacgtttgca ctacgttgag tttttcgctc
     3481 aattttatat gggaggaagt cagaagttta gggccttcag agccaaaaga aagtatactg
     3541 atttaggagg taaataaaat ggtagaaata gctttaggaa cagcattagc agcaatcggt
     3601 gcaggtatgg ccgtaggatt cgctggatta ggatcaggtt tagggcaggg aatagcagca
     3661 gcaggaagtg taggtgctgt ggcagaagat gaagatatgt tcgccagagg tattatcttc
     3721 acagcattgc ccgaaaccca ggctatttat ggttttctaa ttgccatatt gattatggtt
     3781 ttcacgatta tggctggaaa ggtactgcct gcatctttag ggctggtagc aataggagct
     3841 ggtgcagcaa taggatttgc tggtcttggc tctggaatgg gtcaaggtat caccgcatca
     3901 tccgctgtag gggcggtagt cgaaaatgaa gacatgtttg ccaggggtat catatttacc
     3961 gctctatcag agacccaggc tatctacggg ttcttgattg ccatattgct catggtattc
     4021 ggcggaattc tgggatgatg gacatgagtg gggcagataa gatagtctca agtataatgt
     4081 ctgatgctca gattaaagcc gaatcaataa tagaagaagc tgaaaataaa agcatatcca
     4141 ttctcaaaga aggcgaaggt caagcagttt tagagaagga aagaatctta gaaaacgctg
     4201 aaaaacagtc ccaaatgagg taccagcaga ttatctcaga agctaagatg aactcccgga
     4261 gaatggaact tgaggctaga gaaaaaataa ttgaagaagc tttccagaag gctgaagaaa
     4321 agttgaaaag gatcgcttcc tcagatgcac ctgaatacaa ggcatctctt gaaaaagtta
     4381 taaccgaagc tggtgtagaa ataggtggag gtaatttagt agttctggtt aaaaaggaag
     4441 atgaaactaa aatatccagt tctatacctg ccattgagaa atccatcagc gaagagtca
//
LOCUS       DAOQVW010000052         4496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_21517_length_4496_cov_3.079036, whole genome shotgun sequence.
ACCESSION   DAOQVW010000052 DAOQVW010000000
VERSION     DAOQVW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21517_length_4496_cov_3.079036"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(616..1677)
                     /locus_tag="PKK85_01930"
     CDS             complement(616..1677)
                     /locus_tag="PKK85_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HNS24811.1"
                     /translation="MRLVKEDGKRGLIEVVPETLDDLWHLYHIIGPGDLVSSRTTRRI
                     QDNTGERLRSDRGIKKSFFLGIRVENIKFHRYTGKLRATGVIEKGPEDLVPLGSYHTL
                     DLKLQNPIKIQKEEWSRWQRKRVKEAIKSSMIPKALVVVIEGDTADLGILRQYGIDYY
                     GPIIGGISGKRMVMKNRKQALENFYSEVARVVNEQKGVEGIVIAGPGFTKNDFFQYLS
                     QKYPEKASMSRIENTGTGGRVGIREVIKKGVLDEMATEGRIVREIRAMNRILEEIGTS
                     PSSVTYGMNEVKNAAMAGAVEELLVIDNLLRKKDVEKIMNLVENLRGKVMVVSSEHEG
                     GKQLTALGGMVALLRYPLA"
     gene            1820..2140
                     /locus_tag="PKK85_01935"
     CDS             1820..2140
                     /locus_tag="PKK85_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24812.1"
                     /translation="MSQELENRIKEALENVKVWQRVPTSVRGVFLVKAPSKDMENSIM
                     MEINPLDKRGTPIKRRGIFLRQKSHLKSFLEVMNQESVGELLETLEQMTGNSEDDKVN
                     SVEI"
     gene            2171..3475
                     /locus_tag="PKK85_01940"
     CDS             2171..3475
                     /locus_tag="PKK85_01940"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNS24813.1"
                     /translation="MKVSVIGGTRGLGYWIARFLKKNNLMVTITGRDTLAGESAAKSI
                     GVSYTPNNQKAASEADIVIITVPIESTPQIIKDLAPIMKKGSLLIDVTSIKEEPTLLM
                     EKYTPEGVEVLPSHPMFGPRIRSLEGQVVVLTPIKKGKWYPRVVDFLESEKIRVLVTT
                     PEVHDRMMSVVQGLTHFAYISIAATLKRLKVDLKESRKFSSPIYSLMLDMIARIVAQN
                     PYLCYSIQTKNPHIRKTHQAFLETFQELKDMIENENQEEFVKAMSSAAKHLDDIEASL
                     GRSDKAISALNTEIRLLKDSLDQEVGLRHIYSGKIHVGILEDLTPDFLYLKTNKKVLK
                     VKIANIEILKPVELLNWKVRNLPIKTYDISVIFPKRAKATVIAKSVENLRGVLDTEVL
                     DIYQGPPVPHDSQSITIRYRVLDPETILDVEAFLKGFGGIIR"
     gene            3682..>4496
                     /locus_tag="PKK85_01945"
     CDS             3682..>4496
                     /locus_tag="PKK85_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS24814.1"
                     /translation="MKNEEVKLKVAEAFSQADVGRSIARIDPQCMEKLDLVDGDIVEI
                     EGKKITATMVASSQSDIGLEIVRIDGYIRKNAGTSLGEDVIVRKAQIKEAQKVVLAPV
                     DQQIMIRGDVRAAFRGRVMSKGDMIVTGIRQQQQSLRGGLFDEFLRDMSTDFSPMGEL
                     KLAVVSTKPVGIVKITEMTDVEVQSEPVDVSKLEGVKSLVEVTYEDIGVLKEEVKKVR
                     EMVEIPLKRPELFERLGISPPKGVLMHGPPGTGKTLLAKAVANESDAHFIAIQG"
BASE COUNT         1411 a          912 c          888 g         1285 t
ORIGIN      
        1 aaggcaacct ttaagattaa agtcagaatg gcagacagta agaccgccgc tgtaaactgg
       61 atatataaaa gtggcattgt ataattactt gatcttgctc ctttaaaaaa ttaaaaaaat
      121 gtagggatgt ttgttgttca gtgtacttac gaaccgatag ctatgagtat tttttgggta
      181 agagaaacgt attaatagat ttcgtagata ggaagatgtt ccatatttaa caatgggtta
      241 ggctgatgcc tcttggtggg ttgtcaacct taattgggcg cggaccaaat gatttcttga
      301 tgtagtagta agttttttct gtcttggaat tggatccaaa aatctactgt attaaaaggg
      361 ttttctgctt tttactatcg ataatgagtt ctattccaat acagtatttt tcagagaata
      421 atcccagtag agatcaggta taatgcccag ttctggacag gctaataagc aagtaagaca
      481 tttcatgcaa ctccctccac ccattgactc ttaaacattt ctctacatac ataaagccaa
      541 gttgtagaag tcgttttatg cagcgtttct tatccttatt ttttatattc aggggctttt
      601 aaaaaaattt gaccatcaag ccaggggata tctcagaagt gcaaccattc cccccagggc
      661 ggtgagctgt ttacctcctt catgttcact gctaaccacc ataactttgc cccggagatt
      721 ttctactaag ttcatgattt tttccacatc tttcttcctc aataaattgt caataaccaa
      781 taactcctct accgctccgg ccatggctgc atttttgact tcattcattc cgtaggtcac
      841 tgatgagggg gatgtgccaa tttcttccag gattctattc atagccctta tttcacggac
      901 aatacggccc tcagtagcca tttcatccaa aactcccttt ttaataactt ccctaatccc
      961 tactcggcct ccggttccag tattctctat acgggacata ctggcctttt cgggatattt
     1021 ctgtgataaa tactggaaaa aatcgttttt tgtaaatcca gggccggcta tcactatacc
     1081 ctccactccc ttctgttcat ttacaacccg ggccacctcg ctgtagaaat tctccagtgc
     1141 ttgctttcga tttttcatca ccattcgctt acccgagatg ccgccaataa tggggccgta
     1201 gtaatcaatt ccgtactgtc ttagaattcc cagatcagct gtatcacctt caatgaccac
     1261 taccaaagct ttaggtatca tggaagattt tatagcttct tttacccttt ttcgctgcca
     1321 tctagaccac tcttccttct ggatcttaat ggggttctgc aatttcaaat ccaaggtatg
     1381 ataggatccc agtgggacca gatcctccgg ccccttttct atgacacccg tggcacgtaa
     1441 tttacccgta tatcggtgaa attttatatt ttcaaccctt attcctaaaa aaaaactttt
     1501 ctttattccg cggtcgcttc taaggcgttc acccgtgtta tcttgaatcc tacgtgtggt
     1561 tcgggatgaa accaaatccc ctggcccaat gatatgataa agatgccata aatcatccaa
     1621 ggtttcggga acaacctcta ttaatcctct ttttccatct tccttaacca aacgcatgtt
     1681 aagacctcaa atccccttta tgtaagattt tattttatca aaagtttttt atttatatga
     1741 cctttatcaa tcccaccata aaatcctccc atcataactt ttaaataaaa gttattactt
     1801 aatatggtgt ggtgattgaa tgtcacagga actggaaaat aggataaaag aagcattgga
     1861 gaatgttaaa gtatggcaga gagtccccac ttccgtaaga ggagtatttt tagtcaaggc
     1921 tccttcgaag gacatggaaa acagcattat gatggaaatc aatccactag ataaaagagg
     1981 aacccctatt aagcgcagag gaatattttt aagacaaaag tctcatctga aaagtttttt
     2041 ggaggtcatg aaccaggaaa gtgtggggga gttactggaa acgctggagc agatgaccgg
     2101 aaacagcgag gatgataagg taaattcagt ggagatataa tttaataggt ctttcatcaa
     2161 aagattagtt atgaaggtat cggttattgg tggaaccagg gggttaggat actggattgc
     2221 cagatttctg aagaagaata atctgatggt gaccatcacc ggaagagata cactggctgg
     2281 tgagtcagct gctaaatcca taggagtttc ctacacccct aacaaccaaa aagctgcctc
     2341 ggaagctgat attgtcatca taacagtgcc tattgaatct actccacaaa ttataaaaga
     2401 tttggcaccc attatgaaga aaggttctct tctaatagat gtaacatcca taaaagaaga
     2461 acctactctt ttaatggaaa aatacacccc agagggtgtg gaagttttac catcccatcc
     2521 catgttcggg ccccggataa gatccctgga aggacaagtg gtggtgctaa cacccataaa
     2581 aaagggaaaa tggtatccgc gagtagttga ttttcttgaa tcagaaaaaa tccgtgtact
     2641 ggtaactacc cctgaagttc acgaccgtat gatgagtgtt gtgcagggat taacccattt
     2701 cgcctacatc tccatcgccg caacactaaa acgattaaaa gtagatttaa aggaatcccg
     2761 aaagttttca agccctattt acagtttgat gctggacatg attgccagga tagtagcaca
     2821 aaatccctac ctatgttact ccattcagac caaaaaccca catataagaa agacccatca
     2881 ggccttcctg gaaacattcc aagaattaaa ggacatgata gagaatgaaa accaggaaga
     2941 atttgtaaaa gccatgagtt ctgctgctaa acacttggac gatatagaag catcactggg
     3001 aagatccgat aaggctatat cagcattgaa tactgagata agacttttaa aagattcatt
     3061 ggaccaagaa gtgggtttga gacatatcta ttccggtaaa atacatgtcg gaatcctgga
     3121 agatctcaca ccggattttt tgtatcttaa aaccaataaa aaagtattaa aagtaaaaat
     3181 agccaatata gaaatcctta aaccagttga acttttaaac tggaaagtta gaaacttacc
     3241 aattaaaacc tatgatatct ccgttatttt tccaaaacgc gcaaaagcca ccgtaattgc
     3301 aaaaagtgtg gaaaatctaa ggggagtgtt agatacggaa gttttggata tttaccaggg
     3361 acccccagta cctcatgact cccaaagcat taccattcgc taccgtgtgc tggatcctga
     3421 aactatttta gatgttgaag cctttttaaa gggttttgga ggcataatca ggtgattgta
     3481 actgacctaa tccaagtagt ccctcagatt tatcccccag aatgaactta gttccataac
     3541 cacttaatag aaacctttat atactacttc tgacaaggtt aaagtaacat aagttaacta
     3601 aaatatcatg ttaagttaat tttattttta actgcaagaa ttactccgtt taaatgacaa
     3661 taaatatatg ggggttttat tattaaaaat gaagaagtga aattaaaagt ggcagaagcc
     3721 ttctcacagg cagatgttgg gcgttccata gcccgtattg atccacagtg catggaaaaa
     3781 ctggatcttg tagatggaga catcgtagag atagaaggga aaaagatcac tgctaccatg
     3841 gtagcatctt cacaatcaga tattggacta gaaatagttc gaatcgatgg ttacatacga
     3901 aaaaatgcag gaacatccct gggagaagat gttatagtac gaaaagccca gattaaagag
     3961 gctcaaaaag ttgttttagc accagtagat cagcaaatca tgatcagagg tgatgtaaga
     4021 gctgctttcc gcggtagagt tatgtccaag ggagatatga tcgttactgg tatccgtcag
     4081 caacagcaat ccctgcgagg cgggctcttt gacgaattct tacgggatat gtccactgat
     4141 ttcagcccca tgggcgaact gaaactagct gtggtttcca ctaaaccagt tggaattgtc
     4201 aaaataactg aaatgactga tgtagaagtc caaagtgaac cagtagatgt gtccaaactg
     4261 gaaggagtta aaagtttggt ggaagtcacc tacgaggata ttggtgtact gaaagaagaa
     4321 gttaagaaag tgcgggagat ggttgaaata cccctcaaac gccctgaact ctttgaacga
     4381 ctgggaatat caccaccaaa aggggtttta atgcatggtc cgccaggtac aggtaaaact
     4441 ctactagcca aagctgtggc caacgaaagc gacgcccact tcatagctat ccaggg
//
LOCUS       DAOQVW010000053         4446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_21885_length_4446_cov_3.013892, whole genome shotgun sequence.
ACCESSION   DAOQVW010000053 DAOQVW010000000
VERSION     DAOQVW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21885_length_4446_cov_3.013892"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKK85_01950"
     CDS             <1..641
                     /locus_tag="PKK85_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNS24815.1"
                     /translation="REAFGRIVSVVNRIGNELDFLNFAKQQLRNMPTVDTEATTAVIA
                     GFPNVGKSTLLRRITSAEPEVANYPFTTKGIQIGHMERHWKKFQIIDTPGLLDRPVMD
                     MNEIELQAMVALENLADLILFIFDPSTTSGYPLENQINLYNELRQLFKDVTVFPIFNK
                     MDLLGDEENVKYILERINRREEPLMVTASDGSGVSKIIELLDEFDASKEKYD"
     gene            complement(850..1854)
                     /locus_tag="PKK85_01955"
     CDS             complement(850..1854)
                     /locus_tag="PKK85_01955"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNS24816.1"
                     /translation="MEYEMYQEMMEQPQSLRNTLKREKSHLKELASQFKKFDKVYLVG
                     CGSSLSTCYSSKDAANMTFKNSMEVFTGYEFFYHKQIDNEDAGVILTSQSGETADTLA
                     ALRKAKKKGLYTVSITNEGDSSMMNESDDAVLTQCQREHAILGTKTYMTQLLCLYEII
                     FGMDDSKASHDVLTDLEKIPNVVEGLLKATEQDNRILAENYKDQEIFYCLGSGLNYGL
                     AYKLAMTMFMEGALKHACPLYSGEFRHGLIERAEKNVPLLFLDAGYQGDELTRKSIQF
                     GEKIGTHNIIYRMQDYSNLNPLLSPFVLAVPLEWFIYYLAHYNGEDPGSTRHIGKVRY
                     "
     gene            complement(1926..2486)
                     /locus_tag="PKK85_01960"
     CDS             complement(1926..2486)
                     /locus_tag="PKK85_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HNS24817.1"
                     /translation="MELQDIRKALKILESSPEFASLIPEVRSNLVMSKLSPRGIEDVA
                     GIPGRITVINQKPKTCAAPEYGASSHMARLILSIMKHDPEKRSAINMKYNPSLVEICQ
                     KLGLITSYYNRKDEPPKIRDKEGGTIPWGVQKAVERIGRVPDVIYHTGAWGKEPSLVL
                     IGENPLEVVKTAICISKLYIQHSNYD"
     gene            3859..>4446
                     /locus_tag="PKK85_01965"
     CDS             3859..>4446
                     /locus_tag="PKK85_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24818.1"
                     /translation="MLKQHLLLIMLLLLAVTLSAQDLHATPNQQSPENFTNSITNDEI
                     TVNTTSSVKILNSTVNETVSNTNSDQPINTSWTSQTLNSSQYSPNEENNSTANIQNSS
                     IAAGDGTYNNVHGIWLSVDDVKNVKVEELITAGITDVFVKANRISNPTYQSVLTTIIG
                     KLQGTGIRIHAWVVCFVDSDGKWVDPQGNNTYTVKV"
BASE COUNT         1380 a          795 c          843 g         1428 t
ORIGIN      
        1 ggagggaagc ttttgggcgt atagtgtccg tggttaaccg gatcgggaat gaattggact
       61 ttttaaactt tgccaagcag caactccgta acatgcccac ggtagatacc gaggccacca
      121 ccgccgtaat agcaggattt ccaaatgtgg ggaaatcaac cctcctgcgt agaattactt
      181 ctgctgagcc agaggtggcc aattacccat tcactacaaa aggaatccag atagggcata
      241 tggaacgtca ttggaagaag ttccagatca tcgacacccc cggactcctg gatcggccgg
      301 tgatggatat gaacgaaatc gaacttcaag ccatggtggc cctggaaaac ttggcagatt
      361 taatactatt catttttgat ccttccacta cttctggtta tccattagaa aaccagataa
      421 atctttataa tgaacttaga caactattta aggatgtaac tgtatttccc atctttaata
      481 agatggatct cctgggagat gaagaaaatg ttaagtacat tctggaacgt attaacagaa
      541 gagaagaacc attaatggta acagcttctg atggaagtgg ggtatctaaa attatagagt
      601 tactggatga atttgatgct tcaaaggaga aatatgattg aaaagatgtt ttctaaaatg
      661 gtaaatgtca tcaaagaaaa acgagataaa tggagaagtc catagcagaa aatgaatcca
      721 gcaccccatc aaactcagca gggttcagat tacaatggat tggatcgttt taaatatgga
      781 ttaattttat tgaattcgtt ttgctgccat tttcttcgat tttaaattat gaatttataa
      841 aaaaatgaat cagtatctaa cctttcctat gtggcgcgta cttccaggat cttctccatt
      901 gtaatgggcc aggtagtata tgaaccattc caggggcaca gccagtacga atggcgatag
      961 taagggattt aaattggagt agtcttgcat ccggtaaatt atattatggg tgccgatttt
     1021 ctctccaaac tggatcgatt ttcgggttaa ctcatcacct tgataaccgg catctaaaaa
     1081 caataaaggc acattttttt cagcccgttc gattaatcca tgtcggaatt ccccagagta
     1141 taacggacaa gcgtgtttaa gggcaccttc cataaacatg gtcatggcca gtttgtaggc
     1201 cagaccatag ttgaggccgc tgcctaaaca gtaaaaaatt tcctggtcct tgtaattttc
     1261 agctaaaata cggttgtcct gttcagttgc ctttaaaagg ccttccacca catttggaat
     1321 cttttccagg tcggttaaaa cgtcatgaga tgctttggaa tcatccattc caaaaattat
     1381 ttcataaaga catagcaact gggtcatgta agttttggtg cccagaatag catgttctct
     1441 ttggcactgg gttaaaactg catcatcaga ttcattcatc atggaactgt ctccttcgtt
     1501 ggtgatggag acggtgtata atcccttttt tttagccttt cttagggcag ccagggtatc
     1561 ggcagtttct ccggattggg aggttaaaat aacacctgca tcttcattat caatttgttt
     1621 atggtaaaaa aattcgtaac cagtgaaaac ttccatacta tttttgaatg tcatgttagc
     1681 ggcgtctttt gaggagtaac atgtggaaag agagctacca cagcccacca gataaacctt
     1741 atcaaacttc ttaaactgcg atgctaactc ttttaaatga gatttctctc ttttaagggt
     1801 gtttcgcaga gattgaggtt gttccatcat ttcttgatac atttcatatt ccattatcat
     1861 ccctcaaggt tagctagtac aattttgtct agataaattg acccttcata gggtcaaaat
     1921 gttttctagt cgtaatttga atgttgtatg tagagtttag atatacaaat cgcagttttc
     1981 actacttcta ggggattttc acctattaaa actagggaag gttctttccc ccaagcacca
     2041 gtatgatata taacatccgg tacacggcca attctttcca ccgctttttg aacaccccat
     2101 ggtatagttc ctccttcttt atcccttatt ttcggaggtt catcttttcg attgtagtat
     2161 gaagttatta atcccagttt ctggcaaatc tcaactaaag aggggttgta tttcatgtta
     2221 atggcgctgc gcttctcagg gtcatgtttc attatactca ggatcagcct ggccatgtgt
     2281 gaggaagcac catattctgg tgctgcacat gtttttggtt tttggtttat gacagttatc
     2341 cttccaggaa ttccagccac atcttcaatt ccccggggag ataatttact cataaccaag
     2401 ttgctcctta cttctggaat caaggaagca aattctggag aactttccag tatcttcaaa
     2461 gcctttctaa tgtcctgtag ttccattgtt cccttttcta tcttattttt taagcttatt
     2521 tttttttaat tatgttattt aattcaatct attatctggt gatgttgtta gtttagaaat
     2581 tttgattggt ttattttata tgatctcaag acgaggtgtt tatcatttgt atggtaacca
     2641 tatctatttt aaatattata aattatccct ttaagtctaa cttttatatt ctttttttta
     2701 taaattaggg ttaataataa ctagtaaggg agaattgggg ggtgtatttc ttaataaata
     2761 ctttaagaat cttactttac tagggtatgg aattgctagt actttttcac taaatactgc
     2821 ttttaattcc acaaaaacag gcattataaa ttcctaccag taaaaccata gaacacaaca
     2881 tgtgttatgc tttgcgagat aacgggaaca tttttttcta ctaaccagcc atggcaatag
     2941 ggattctctg tcaattaacg gacaataggt tcgaagtgaa tataacagtt tttctattgc
     3001 ttaaattaga tgatcataaa gctaattatt ttttacaagc tgtgaaaatc cggaaaaaat
     3061 tttaccactc tccattaaga agtttggtag atattcggac tatacatgtc ctatcgttgg
     3121 tttagagtta gggattccat ggaaacagtg ggttatcatc ttttttcata acaagatggc
     3181 aaccatcaac tgctaggttc attggagttg ccgattgggg gggtcatgat gatactatca
     3241 aggagatttt actcatcaag tggcccaaat atgaaaagat tacaacccat caggcgaaac
     3301 attctggtgg ataaaaaaaa gatgataaga atctttgaac ataaatacat gattataaaa
     3361 gttcatcgtg cttgttagaa gtatgtgtgg tagattaatt ctaaatgcaa taaggataca
     3421 tatggaaggc ggatttaact taaaatttag aatcttttta tataaaatct gttgttatat
     3481 cgtttttttc ttaaaggata accgataata attttaaaat tggaaataat caatcgccat
     3541 tatctcaatg ttaaataagg taaatgttgt attctaagaa caatattggc atttaataca
     3601 aaaaatttga gagatttgat aaattttcca aaattaaaca gttattgttc atgttctggt
     3661 gcatatgata taaaaatgac acctataata gatgaatagg gataaataaa tatttaaaat
     3721 gttttatttt ctcttaaaac aatttaaaga gcttatttgt ctgaattttc tataataaaa
     3781 tatatatgct ccaagattcg atcttattgt gtcaggaact ctaattgttc cactgacttc
     3841 gaagatagag ggggcatagt gttgaagcaa catttgttgc tgataatgtt gctgctgttg
     3901 gcagtgacgt tgtccgcaca ggatttacat gccaccccaa atcagcaaag tccagaaaac
     3961 ttcacgaatt caataacaaa cgatgaaatt acagttaata ccacttcatc agtaaaaatc
     4021 ctcaattcga ccgttaatga aacggtcagt aatactaata gcgatcagcc cattaataca
     4081 tcttggacat ctcaaacact caactcgtct caatattcgc caaatgaaga aaacaacagc
     4141 acagcgaaca ttcagaactc cagcattgct gcaggagatg gaacctacaa taatgtccac
     4201 ggaatatggc ttagtgtaga tgatgtgaaa aatgtgaagg tcgaagaatt gatcacagcc
     4261 ggaataacgg atgtgttcgt caaggccaat cgaatatcaa accctacata tcaaagtgtc
     4321 ctgacaacta taattggcaa actacaaggc actgggataa ggatacacgc ctgggtggtc
     4381 tgtttcgtcg atagtgatgg aaaatgggtg gatccccagg gtaataatac ctacaccgtt
     4441 aaagta
//
LOCUS       DAOQVW010000054         4441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_21914_length_4441_cov_3.570679, whole genome shotgun sequence.
ACCESSION   DAOQVW010000054 DAOQVW010000000
VERSION     DAOQVW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21914_length_4441_cov_3.570679"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..841)
                     /locus_tag="PKK85_01970"
     CDS             complement(53..841)
                     /locus_tag="PKK85_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS24819.1"
                     /translation="MIFRMIKSEGLAHHSYFLADGGKAAVIDPRRDVEVYLDLAREND
                     LQISYIFETHRNEDYVIGSLELQKASGGEIFHGEHFDFAYGTPVKEKDKFRLGTMELE
                     ILETPGHTKESISLVLRDLSVGSDVQMVFTGDVIFTGETGRVDFYGIKSRPNMAELLY
                     DSLFAKIFPLGEHVILCPAHGAGSVCGADIREQEYTTTGYEIKTNPQLQLSKEEFIRQ
                     KSEEELYYPPYFRKMEELNQYGAPIVGRVPTLRPFSVRTGTTNE"
     gene            984..1928
                     /locus_tag="PKK85_01975"
     CDS             984..1928
                     /locus_tag="PKK85_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS24820.1"
                     /translation="MEYIIETKDITKKFDDFTAVDSVNLKIPTNSVYGVLGPNGAGKT
                     TLISMLCTILRPTHGSATVNGYDIVKNPKEVRASIGIVFQSRALDDILTGREHLEMHA
                     ALYGVPKDLREKRLEEVLDLIALGSKVDEFVKTYSGGMKRRLEIGRGLIHHPKVLFLD
                     EPTLGLDPQTRESIWEYIQRLNQEEEVTVLMTTHYMEEADKLCDEVAIINQGNIITAD
                     SPSNLKRELKADTITIRVDKAEEFLERAKSLAFVKDIFEADSEIKIMVERGDYLVPEI
                     VNFANQHNIFVNSIELEHPNLEDVFLKYTGRTIGEGGR"
     gene            1931..2695
                     /locus_tag="PKK85_01980"
     CDS             1931..2695
                     /locus_tag="PKK85_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS24821.1"
                     /translation="MTELEGIYTIWLRENKRFVRYRSRILTSVVTPLLWLLIFGTGLG
                     SAVRFGGVVGGYKTFIYPGIIGQTILFTAVFSGVSVIIDRQYGFLKEILVAPISRPAI
                     VFGKALGISTTALIQGLILLLLSFVVGIQMTIEIFLQSAVVILLISLGLAGLGLLIAS
                     FTDSMEGFNLILSFIVLPIFLLSGALFPVTNLPSWLQIAVYLDPLTYGVDALRAIILK
                     TSVLPLYLSVAIVSLFTILMVLTSAFIFSRREQELM"
     gene            3272..3499
                     /locus_tag="PKK85_01985"
     CDS             3272..3499
                     /locus_tag="PKK85_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNS24822.1"
                     /translation="MPDCHKCGTKNPEGAQFCSRCGTKLTIFPRLEKKSRKPENSHSE
                     LKIQVILALIVLILIIVAILMSFIPGVGIKL"
     gene            complement(3545..4438)
                     /locus_tag="PKK85_01990"
     CDS             complement(3545..4438)
                     /locus_tag="PKK85_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNS24823.1"
                     /translation="MKMKIEPMLAKLESLDVNLEGVWISEPKYDGERILAEAKDKKIG
                     LWTRRHVQVSRKFPEVVTALLKVEGDDWVLDGELTVTGGFRQLLKRNVEDKTKIKILS
                     QKIPSTYHVFDVLRWEGVDITTQPLKERKKVLLAHIKPSNRIKVMPFRLVTNIDVEKH
                     FREYLNQGYEGTILKNVFSPYEAGKRTGQWIKIKRDETVDVNIIGATRSTGSIPFGAL
                     LMEKDGKYFGKVGTGYSSAEQKFIMKILQENPSPPTIPLPRDVASEVLITSLPIPAEI
                     RVNEIYKGSPRAPVWVRFRWG"
BASE COUNT         1245 a          966 c          948 g         1282 t
ORIGIN      
        1 tatatgacca ccgccgaagc tggttggttt ccggatgtct aaaatctgga agtcattcat
       61 ttgttgttcc agttctgacg gagaagggtc ttagagtagg gactcggccc acaatgggag
      121 ctccatactg attcaactcc tccatcttcc tgaagtaagg tgggtaatac aattcttctt
      181 cagatttttg gcgtataaat tcttctttac ttaattgaag ctgaggattg gtttttattt
      241 catagccagt agtggtatat tcttgttccc ttatatcagc gccacaaact gaccctgcac
      301 catgggctgg gcacaggatc acgtgttcac ccagtggaaa tattttggcg aacaagctat
      361 cgtagagtaa ctcggccata ttaggccggg atttaattcc gtaaaagtca actcttcctg
      421 tttctcctgt gaagatcacg tctcctgtaa aaaccatctg aacatcactg ccgacactta
      481 aatccctaag aaccagagat atgctctctt tggtgtgccc aggagtttcc agaatctcta
      541 attccatggt tcccaatcga aatttgtctt tctccttaac tggagttcca taggcaaaat
      601 caaagtgttc gccatggaat atttcaccgc cactggcctt ctgaagctcc agagaaccaa
      661 taacgtaatc ctcattgcga tgggtctcga agatgtagct tatttgaaga tcattttccc
      721 gggccagatc caagtagacc tccacatcac gtcgaggatc aattacagct gcttttccac
      781 catcggctag aaagtaagag tgatgggcta gcccctctga ttttatcata cgaaaaatca
      841 agttttaacc acctatatga ttactagaat ttctaggttg attataattt aatcaggatt
      901 tgatttaatt ttatcgatgg aaactaaata attaaatcac cccggtacac attattttta
      961 tccagagatt aggggaggtt ctcatggaat acattatcga aacaaaggac atcaccaaaa
     1021 aattcgatga tttcactgca gtggattctg taaaccttaa aattcctaca aacagtgttt
     1081 atggagtttt aggaccaaat ggggcgggta agacaactct gatttccatg ttgtgcacca
     1141 tactacgccc cacccatgga tcggccacgg tgaatggata tgatatcgtt aagaatccta
     1201 aggaggtaag ggcctccatt gggattgtat tccaatcccg ggcactggat gatattttaa
     1261 cgggcaggga acacttggaa atgcacgctg ccctctacgg tgtccccaag gatttacggg
     1321 aaaagcgtct ggaggaggtg ctggatctga tagccctggg atccaaggtg gacgaattcg
     1381 tgaaaaccta ctccggtggt atgaagcgga ggttggaaat tggaagggga ttaatacacc
     1441 atccaaaagt cttgtttctg gatgaaccta cgcttggcct tgatccgcag accagggaaa
     1501 gtatctggga gtacatccaa agattgaacc aggaagagga agtcacggtc ctcatgacca
     1561 cccactacat ggaagaggca gacaagctat gcgatgaggt tgccatcatc aaccagggaa
     1621 acatcatcac cgcagactca cccagcaacc ttaaacggga gttaaaagcc gacaccatca
     1681 ccatcagggt ggataaagca gaagaatttt tagaaagggc caagtccctg gcattcgtaa
     1741 aggacatctt cgaggccgac tcggaaataa agattatggt ggaacggggg gactatctgg
     1801 tgcctgaaat agttaacttc gctaatcaac ataatatctt cgtgaactcc attgaactgg
     1861 aacatcctaa cctggaagac gttttcctca aatacactgg aagaaccata ggtgaaggag
     1921 gcagataaaa atgacggaac tcgagggaat ctacacaatc tggctccgtg aaaataaaag
     1981 gttcgtaagg taccgatcaa ggatcttgac ctcggtggtc acaccccttt tatggttgct
     2041 tatcttcggc acgggtctgg gttcagccgt ccgttttgga ggtgttgtgg ggggatataa
     2101 gaccttcata tacccgggaa tcataggtca gaccattctt ttcaccgctg tattttcggg
     2161 ggtttcagtg attatcgacc gccaatacgg attccttaaa gagatattgg tggcacctat
     2221 ttccaggccg gcaattgttt tcggtaaagc actgggaata agtaccacgg ccctgataca
     2281 gggcctgata ctactgttac tatccttcgt ggtaggtatc cagatgacaa ttgaaatatt
     2341 tctccagagt gcagtggtca ttctactgat ctccctgggt ttagctggat taggactgct
     2401 aatagcctct ttcaccgata gtatggaagg atttaacctc atcctcagct tcattgtact
     2461 ccccatattt ttactaagcg gagccctgtt cccggttacc aacctgccat cctggcttca
     2521 gatagcggtg tacctggatc ctttaaccta tggggtggat gccctccgag ccataatcct
     2581 gaaaacatcg gttttacccc tctatctaag cgtggcaatt gtgagtctgt ttacaattct
     2641 catggtcctc acatcagctt tcatcttcag tcggagagaa caggagttaa tgtagcttat
     2701 ttttcggtta aataattccg gtaattatat ttaagaaaaa tattaagtgg aataattaat
     2761 atcccccgga ccaagagata aaaagaagtg aagcactatt cctgcaagaa aatgtgtgta
     2821 tttagggctg gtttatcctt cttccagtgc agccacctaa agatcagagt agagataagc
     2881 ataagggaag tgaaaagacc gctgatagca cagttactgg agacgtgttc atctgcagct
     2941 ttttggcgag gggtatatcc agtaaatgct taacttgatc aacaagggtt atacacatac
     3001 taagaaacat ttccatagcc tttaatccac tatattccaa taacaatgac aataaaagat
     3061 tatttcatgt taatatttac tggaatcctt tgaataattt tcttgaagtt gctattaata
     3121 atggaattct gtgcagatga tgatcatggc ggctgataag tattatatcc atcaaattat
     3181 aaagcgaatt caaaggatat atgatgatat aacagaaact cgaaatacga tataaccaat
     3241 ggtaattgat cctaacaatg gaggatgcct gatgcctgat tgtcataaat gtggaacaaa
     3301 aaacccagaa ggggcccaat tttgctcgag gtgcggtacc aaactaacaa tctttcctcg
     3361 actggaaaaa aagtccagaa aaccagagaa tagccattca gaattgaaaa tccaagtgat
     3421 cctggcatta atagtcctga tcttgatcat agtggctatt ttaatgtcat tcatccctgg
     3481 agtgggcatt aaattgtaaa gggaagatta tacccaagaa tcatccttca ttccttactt
     3541 ctacttaccc ccaccgaaat ctcacccata cgggagcccg gggtgaaccc ttgtatattt
     3601 catttactct tatctcagcg ggtataggta gggatgttat caggacctcg gaggccacat
     3661 ctcgtggtaa ggggatagta ggggggcttg gattttcttg gaggatcttc attatgaact
     3721 tttgttcagc tgaactgtat ccggtgccta cttttccaaa gtattttcca tccttctcca
     3781 tcaggagtgc tccgaagggt atgctgccgg tgctacgggt ggcaccgatg atgttaacat
     3841 caactgtttc atctcttttg attttaatcc attgaccagt tcttttaccg gcttcgtagg
     3901 gtgaaaacac atttttaagg atggttcctt cataaccctg gttcaagtac tccctaaaat
     3961 gtttttcaac atctatatta gtcactaaac ggaaaggcat aacttttatc cgatttgatg
     4021 gcttaatatg ggctaaaagt actttttttc tttctttaag gggttgggtg gtgatatcca
     4081 ctccctccca tcggagtacg tcgaaaacat ggtaggtgga tggtatcttc tgggacaaga
     4141 tttttatctt agttttatct tccacgttac gctttaaaag ctgacggaag cctccagtca
     4201 cggttaattc cccgtccagg acccagtcat caccttcaac tttcaggagt gcagtcacca
     4261 cttccggaaa cttcctggat acttgcacat gtctacgggt ccacagtcca atttttttgt
     4321 cctttgcctc ggctaaaatt ctttccccat catatttggg ttcacttatc catacgccct
     4381 ctaaattcac atctaaactt tccagtttag ccagcatggg ctctatcttc attttcatcc
     4441 a
//
LOCUS       DAOQVW010000055         4397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_22207_length_4397_cov_3.616536, whole genome shotgun sequence.
ACCESSION   DAOQVW010000055 DAOQVW010000000
VERSION     DAOQVW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22207_length_4397_cov_3.616536"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PKK85_01995"
     CDS             complement(<1..195)
                     /locus_tag="PKK85_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNS24824.1"
                     /translation="MDLMILSVVVLIYLLMVGYVGYIAWKRTKSADDYMVAGRKTHPY
                     IMALSYGATFISTAAIVGFGG"
     gene            421..1170
                     /locus_tag="PKK85_02000"
     CDS             421..1170
                     /locus_tag="PKK85_02000"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNS24825.1"
                     /translation="MLEPSRKKRDENKKGFLSKGARLLKPPRKGMDKQKSIPKVSLGK
                     KGPISRKDLISQKVHEKGGFISANRRLVFILTAIICLVVVFALYSGSEAGNETSTPPS
                     NNTQTSANFNVYDNENITFNYPLNWNVSDETEFPVMVTVYKDNNNLLSVFSEELGNMT
                     LYDKLVKWKSNLLQTSNITYEQAITVDNTRAYDVESSIKSDSTTYVTRGVAFEKDRRV
                     YFLVFVFNQSLLNYKDDMELILNSFHVKESS"
     gene            1203..2507
                     /locus_tag="PKK85_02005"
     CDS             1203..2507
                     /locus_tag="PKK85_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNS24826.1"
                     /translation="MIWNEEAECMDQKKREELQLKRLQTVVKRAYENVPHYKNRLDQV
                     GVKPDDIKTLKDIEKLPFTSKEDLRESYPFGMFAVPPREIVEIHTSSGTTGKPVVSGY
                     TRKDLDTWSEVMARGLTMFGADPDDIIQNTHGYGLFTGGFGVHYGAQKVGCTVIPIST
                     GQTKRQIEIMEDFNSSIVIVTPSYGLYLAEVAVEEGLDTEKLKLKAIGFGAEMWTEGM
                     RRELQNRFHAPAYNIYGLTEIMGPGIALECQEQDGLHVMEDHFYPEIIDPETLENVEE
                     GETGELVLTNLTREGMPIIRFRTKDLTALRRGKCSCGRSLIKMDRITGRTDDMLKVRG
                     VAVFPSQVEKALLKIDGIEPHYQIIVTRPQHLDEMEVRVETSPELFSDEVKELVGAKK
                     RIENYIHDEIGLRVKVTLVEPKTIPRSEGKAVRVFDKRKFDN"
     gene            2520..2948
                     /locus_tag="PKK85_02010"
     CDS             2520..2948
                     /locus_tag="PKK85_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HNS24827.1"
                     /translation="MKIKQLSIFLENRKGRMRKALDVLEKGQVSIRALSLADTSDFGI
                     LRLIVPEPEGTKKLLEDHDFIVKTGKVVAVRMEDEPGSLGKILGILDENDINLSYLYA
                     FVAKEDMAIVLLHPENIEEGIGALERGGADLISAQEIYEL"
     gene            complement(3002..3391)
                     /locus_tag="PKK85_02015"
     CDS             complement(3002..3391)
                     /locus_tag="PKK85_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24828.1"
                     /translation="MDKKVMALILVVVLVVAGAAYLLGTQSRPAVNNTTNQTNTTPIH
                     NGTNQATNQTPKVNITAQQAQKIAIDASADLGYPAKAHGTPVLFKWTKNKLHTWVWDV
                     PLEFESGSTKYGSFYVDAYTGVIVMNE"
     gene            complement(3456..4043)
                     /locus_tag="PKK85_02020"
     CDS             complement(3456..4043)
                     /locus_tag="PKK85_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNS24829.1"
                     /translation="MKAYSIYISGVGGQGIIKTSVVIGEAAMKTGQEVVMSEIHGMAQ
                     RGGGVSTELKIGHAHSSIIADGTGDLLIAFEPLEALRALPKTSKNTHVVMNTSKILPF
                     NIQASEHPYPPLEDILKEMNSKSKDVKALDAVKIATQAGHILSMNMVMLGAALTVPGF
                     PLDKETLLDSMKANLPERTIPINTRAFEEGFATFR"
     gene            complement(4040..>4397)
                     /locus_tag="PKK85_02025"
     CDS             complement(4040..>4397)
                     /locus_tag="PKK85_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS24830.1"
                     /translation="AVGVEYMRIINPLNIKKSVKIFQEALEHPGVAVVISKHPCVLLK
                     TRPRQGATQLVVDSQKCDDCGECLSTLACPALYQGEGQVKIDPQLCHKCNVCVQICPQ
                     GAIKGERLDKDREEKP"
BASE COUNT         1252 a          974 c         1020 g         1151 t
ORIGIN      
        1 ccccccaaaa ccaactatag ccgcggtgct gatgaatgta gcaccataac tcagggccat
       61 gatgtaggga tgggtcttac gaccggctac catgtaatca tcggcacttt ttgtacgttt
      121 ccaagctatg tagcccacat atcctaccat taaaaggtaa atcaagacaa caacacttaa
      181 tatcatcaaa tccatttcct aggcctccgt aatagatatt gactgttata agccacatcc
      241 cataattata atgattctta atttacattt tgaataatat ataatccttg ggaaatgtcg
      301 ctaattttat acgcccccat caaaatagta tctttaaaat agtacattag acaagaatta
      361 ctcacatcat catgagaaca tatcaaccag cgcattaaaa ctcttcaagg cagtgataag
      421 gtgctagaac cttccaggaa aaaacgggat gagaataaga aaggtttcct ctccaaggga
      481 gcgcggctgc tgaaacctcc caggaaaggg atggataaac agaaaagtat tccaaaagta
      541 agtctaggta aaaaaggccc aatatcccgt aaagatctta tttcccagaa ggttcacgaa
      601 aaagggggct tcatatcggc caaccgcagg ttagtcttta ttcttactgc aataatttgc
      661 ctagtagttg tgtttgccct gtattcaggt tctgaagccg gtaacgaaac cagcacccca
      721 ccatccaaca atacccagac cagtgccaac ttcaatgttt atgataatga aaacataacc
      781 ttcaattacc ctttaaactg gaatgtgtcc gatgaaacag agtttcccgt gatggtgacg
      841 gtgtataagg ataacaataa tctcctctct gttttttctg aagagctggg aaatatgacc
      901 ctttatgata agctggtgaa gtggaagtcc aacctcctcc agaccagcaa catcacctat
      961 gagcaagcca taaccgtgga caacacccgg gcctatgatg tggaatccag tatcaaaagc
     1021 gattctacaa cctacgtcac ccggggcgtg gcttttgaaa aagatagaag ggtttacttt
     1081 ttggttttcg tatttaacca gtcactctta aactacaaag acgatatgga actaattcta
     1141 aacagtttcc atgttaaaga atccagctaa attattaatt agaaatctaa cgaggattaa
     1201 caatgatctg gaatgaagaa gcagaatgca tggatcaaaa gaaaagagaa gaattacaac
     1261 ttaaaagact gcaaacagtt gttaaaagag cctatgaaaa tgtgcctcat tataaaaatc
     1321 gtctggacca ggttggagtg aaaccagatg atataaagac cctgaaggat attgagaaac
     1381 ttcccttcac ctccaaggaa gacctgaggg aaagttaccc ctttgggatg ttcgccgtgc
     1441 ctccccgaga gatcgtggag atacacacct cctccggcac caccggaaaa cccgtagtat
     1501 caggttacac ccgaaaagac ctggatactt ggtcggaagt tatggctcgt ggccttacca
     1561 tgttcggagc agatcctgat gacataatcc agaacacaca tggttatgga cttttcaccg
     1621 gaggctttgg agtgcactac ggtgcccaga aggtagggtg caccgtgata cccatcagca
     1681 ctgggcagac caaaaggcag attgagatca tggaggactt caacagcagc atagttattg
     1741 tcaccccctc ctacggcctt tacctggcgg aagtagccgt ggaggaaggt ttggataccg
     1801 agaagttgaa gctgaaggcc atcggatttg gtgcagagat gtggacggag ggaatgcgcc
     1861 gggaactgca gaaccgtttc catgcacctg cctataatat ttacggcttg accgagatca
     1921 tggggccggg gattgccctg gaatgccagg aacaagatgg tttgcacgtt atggaggacc
     1981 atttttatcc ggaaataatt gaccccgaaa cccttgaaaa cgtagaagaa ggagaaactg
     2041 gtgaattagt tcttaccaac ctcaccaggg agggcatgcc cattatccgc ttcagaacca
     2101 aggatttaac tgctctgcgc cgggggaaat gttcttgtgg acgtagcttg attaagatgg
     2161 atcgtatcac tggccgtacc gatgacatgt tgaaggttcg gggagtggct gtgttcccat
     2221 cccaggtgga gaaggccctc ctcaaaattg atggcatcga gccacactac cagatcatcg
     2281 tgacccggcc ccagcacctg gatgaaatgg aggtgagggt ggaaacctcg ccggaactct
     2341 tttcagacga ggtcaaggag ttggtgggtg ccaagaagag aattgaaaac tacatccacg
     2401 atgaaatagg tttacgagtt aaggtaaccc tggtggaacc taaaacaatt cccagaagtg
     2461 aagggaaggc agttagagtt tttgataaaa ggaaatttga taactaaggg ggaatgaggt
     2521 tgaaaataaa acaactatcc atatttctgg agaaccggaa aggacgaatg cgaaaagcac
     2581 ttgatgttct cgaaaagggc caagtgagta tcagggcact ttcactggct gatacctctg
     2641 attttgggat tttaagactc atcgtccctg agccagaagg gactaaaaag ttactggaag
     2701 accatgactt catagttaaa accggaaagg ttgtagccgt tagaatggag gatgaacccg
     2761 ggagtttggg caagatcctg ggaattctag atgaaaatga tataaacctg tcctacctct
     2821 acgccttcgt agccaaggag gatatggcca tcgtactttt acacccggaa aatatagagg
     2881 aaggtatagg ggccctggaa aggggtggag cggatctgat ctctgcccag gagatttacg
     2941 aactgtaaaa tctctccatc catttttttt ggggatgagg ggaaagaaat aaaatttttt
     3001 tttattcatt cattacaatc acaccagtgt aggcatccac gtagaaggaa ccgtacttgg
     3061 tagatccaga ttcaaattcc agtggaacat cccataccca ggtatgtaac ttgtttttgg
     3121 tccatttaaa cagcaccgga gtaccatggg ccttagccgg atatcctaga tctgcagatg
     3181 catcaatggc tatcttctga gcttgttgtg cggtgatgtt aactttaggg gtttgattag
     3241 tggcttgatt ggtgccgtta tgaatgggag tggtattggt ctggttggtg gtgttattta
     3301 cggcgggtct tgactgggtc cctaaaaggt atgcggcacc ggctaccacc aacaccacca
     3361 ccaaaataag ggccataact tttttgtcca tatttttttt actcctgatt tgattcttcc
     3421 tttttttaat tatttattgt acccgtctta aaaaattatc tgaaggtggc aaaaccttcc
     3481 tcaaaggccc gtgtattgat aggtattgtc cgttctggta agttagcctt catggagtcc
     3541 aataatgtct ctttatccag aggaaatcct ggtacagtta atgctgcacc cagcatcacc
     3601 atgttcatgg agaggatgtg accagcctgg gtggctattt tcacggcatc cagggcctta
     3661 acatccttag atttagaatt catctccttc aaaatatctt ccaggggagg ataggggtgt
     3721 tcactggcct ggatgttgaa gggcagtatc ttggaggtgt tcataaccac atgggtgttc
     3781 ttacttgtct tgggcagagc ccgaagtgcc tccaatggtt caaaggctat aagtagatcc
     3841 ccagttccat cagctattat agagctgtga gcatggccta ttttgagttc agtggagact
     3901 cctcctcccc tctgagccat accatgtatt tcactcatca ccacctcctg gccggttttc
     3961 atggcagcct caccaatgac caccgatgtt tttattatgc cctgaccccc tacaccggat
     4021 atgtagatgc tgtaggcctt catggttttt cctccctatc tttatccaac ctctccccct
     4081 ttatagctcc ctggggacat atttgtacac atacgttgca tttatgacat aattgcgggt
     4141 ctatcttcac ctggccctcg ccctggtaga gggctgggca ggccagggtg gagaggcatt
     4201 ccccgcagtc atcacacttc tgggaatcca ccaccaactg ggtggctccc tgccttggcc
     4261 tggttttaag gagtacgcag ggatgtttgg atatgaccac cgctactcca ggatgttcca
     4321 gagcttcctg gaatattttt acagattttt taatattcag gggattgatg atgcgcatat
     4381 actccacacc caccgcc
//
LOCUS       DAOQVW010000056         4391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_22249_length_4391_cov_2.806042, whole genome shotgun sequence.
ACCESSION   DAOQVW010000056 DAOQVW010000000
VERSION     DAOQVW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22249_length_4391_cov_2.806042"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..261)
                     /locus_tag="PKK85_02030"
     CDS             complement(49..261)
                     /locus_tag="PKK85_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24831.1"
                     /translation="MTLKYLEKDQERVDKKLEELKIKLDELEHQARLQRLERKYEKLK
                     ASGKPGDNLMSELYQDLIKLEKKVKR"
     gene            complement(258..1748)
                     /locus_tag="PKK85_02035"
     CDS             complement(258..1748)
                     /locus_tag="PKK85_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS24832.1"
                     /translation="MSNLSFYGGVDEIGGNKIQVSSRESSFFFDFGLAFSRANTYLSE
                     FLQPRKANGILDFVALELLPCLKGIYREDYLRHVGLSHTREPAVDGVLISHSHVDHVS
                     LVHHLREDLPLHLSQGSYLILRALEETGSSSFSEYLQLKKSFQLQPKKRGEGYTRRTI
                     PVDREINLTRPYQEFQVGDFTIQAAPVDHSLPGASAYLAENDDGMLVYTGDLRFHGQH
                     PELTHQFVEKARKAQPTTLICEGTRINSTGNTSEEDIQRKAQEVVDHYPGLVVVNYPV
                     RDLDRLLTFHRVACNTDRQLVVSLKQAYILDLFQGSDYPELGEVLVYQPKKGWGMVSE
                     ESYACRDEEWVCARDLDPEISQRDYQTWEREYLDQDYTINYQDLQEYPEDYIFRCDFF
                     ELKELIDIKPERGVYLKSMTEPFDEQMEVNEEKVKSWLRLFNLPLLRGFHASGHASGG
                     EILDMIRRINPEEVYPIHTEGKEQFKVLEEDGIKVKYPTLSKGGLS"
     gene            2715..3020
                     /locus_tag="PKK85_02040"
     CDS             2715..3020
                     /locus_tag="PKK85_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNS24833.1"
                     /translation="MVYCHNCGTKNEDDVEYCSKCGTPLKDDVERRHHQKRQRDECFG
                     LPYGNLIGPLIIGVILILAGLSSIYGFPFWQYLWPALIVLIGILIIAGAIYKATHRD"
     gene            complement(3062..3697)
                     /locus_tag="PKK85_02045"
     CDS             complement(3062..3697)
                     /locus_tag="PKK85_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNS24834.1"
                     /translation="MNSNRLLSFLSLAGLLFLVLMYLWVPAAKTSKTLVLAVFILVCV
                     LGMMAAGYPHHCLRLLNREREKSEPSGHHPVCSEFETHTFTLQGKRYCAGCSGLFLGG
                     ALAILWCLFYYLYGFSSPILFWAGVLAVFLALMQLNYSKIDRAPVKFLSNLVLVTGAV
                     MILVGILEFKANLSPYFLVLIGLWIYTRTTISARDHDLICRQCSIPACPYR"
     gene            complement(3690..>4391)
                     /locus_tag="PKK85_02050"
     CDS             complement(3690..>4391)
                     /locus_tag="PKK85_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNS24835.1"
                     /translation="QEQGINFHWLTDDNLGLGPHTRKLCDELIKRGLSEDVKWFVQAR
                     SDAIIKNQDVLPELRKAGNYWVMAGLERHDPATINSYHKGIRTEDSRLAMNLLKDHDI
                     FAQATFITGDRSDSHQSMEEFREFVNYVDPDIAIFMILTPFPGTELYETAQRNGWLED
                     YNWSNYDMVHAVMPTQHLTRNEVQEELFLCYRDFYGSVKRRLTGVFSKNQFKRMTYRY
                     MAGQGLLQSLRGLFE"
BASE COUNT         1071 a         1109 c         1060 g         1151 t
ORIGIN      
        1 tcaattatga gaatagagtg gccagggtaa aaagatggta ggagaatgct agcgcttcac
       61 cttcttctcc agcttgatca aatcctgata caactcggac attaaattat cccctggttt
      121 tcctgatgct ttcaactttt catacttcct ctccagtcgc tggagcctgg cctggtgttc
      181 cagttcatcc agcttaatct tcaactcttc tagtttttta tcaactcttt cctggtcctt
      241 ttccaagtac ttaagggtca tgataggcct cctttactta gtgtgggata tttgactttg
      301 atcccgtctt cttcgaggac tttgaactgt tctttgccct cagtgtggat ggggtacact
      361 tcttccgggt ttatcctccg gatcatatcc aggatctccc ctccactggc atggcctgag
      421 gcatggaagc cccttaagag gggtaggttg aacagtcgaa gccagctttt caccttttcc
      481 tcgttgacct ccatctgttc atcaaagggt tcggtcatgg atttgaggta aacacccctt
      541 tctggtttga tgtcgattag ttccttcaat tcgaaaaagt cgcagcggaa gatgtaatcc
      601 tccgggtatt cctgcagatc ctggtagttg atggtgtagt cctgatccag gtactccctc
      661 tcccaggtct ggtagtccct ttgacttatc tccggatcca ggtccctggc acaaacccac
      721 tcctcatccc ggcaggcgta gctctcctca ctgaccatac cccacccttt tttgggctgg
      781 taaaccagca cctcccccag ttcggggtag tcactcccct ggaagaggtc caggatgtag
      841 gcttgcttga ggctcaccac caactgccgg tccgtgttac aggccaccct gtggaaggtt
      901 aaaagcctgt ccaagtccct taccgggtag ttaaccacca ccagcccggg gtagtggtcc
      961 accacttcct gggccttcct ctgtatgtcc tcctcactgg tgttgcctgt gctgttgata
     1021 cgggtgccct cacatattag ggtggtgggc tgggctttac gggctttttc aacgaattgg
     1081 tgggttaatt cggggtgctg tccatggaag cgcaggtccc cggtgtagac cagcattcca
     1141 tcatcatttt ctgccaggta agccgacgcc ccgggcaggc tgtgatccac tggtgcagct
     1201 tggatggtga agtctcctac ctggaactcc tggtagggcc gggtcaagtt gatctcacgg
     1261 tccacaggga tggtcctacg ggtgtagccc tctcccctct ttttgggctg gagctggaag
     1321 gattttttaa gctgcagata ctcggagaat gaggaggacc cagtctcctc cagggccctt
     1381 aatatgaggt aggacccctg ggagaggtgc aggggcaggt cctcccggag gtggtggacc
     1441 agggacacgt ggtccacgtg gctgtggctg atgagcaccc catccactgc tggttccctg
     1501 gtgtggctga gacccacgtg gcgcagataa tcctcccggt agatgccctt caggcagggt
     1561 aagagttcca gggccacgaa gtccaggata ccgttggcct tacgtggctg caggaattcg
     1621 gataggtagg tgttggcccg ggagaaggcc aggccgaagt caaaaaagaa ggatgattcc
     1681 cggctgctaa cctggatctt gttaccaccg atctcatcca ccccaccgta gaaggataaa
     1741 ttgctcatta ctattgtttt atcatagggg ctgattatag taattttcta ggtaaggata
     1801 tttatttcta atccgcactt attggtgaaa tgtaaaccct agattaggtc caggtaaggg
     1861 tcgaaaattc ttagaagcac ccccttttaa gagttgaaga tggggataat ccttaaaact
     1921 tatgggggtc ctggaagatc tggattctat ctttaatgga tgaaatatcc atcctcccca
     1981 ctggggcatg tgcttttaaa ccactgatac agtgtatcag acttgtagtt ggcaggggaa
     2041 tttttagtaa aagaaacgtt actagaaata atacgataaa aaaaaactga ggtttcttaa
     2101 caaaacccta acacaagaca tttattccta tccaagcaat cctgagatgt taacttgtct
     2161 ttctaggcaa ctggcagcac cttcccctaa ccccggtgtt tttccagaca gccacctgac
     2221 cagaaccccc tccacaccat gatcctattc atccaggttc aggtcgcatt cggtgcagaa
     2281 gctggtatca gacgatactc tggcattaca tattcgacag taggtaaagt atgatgaatc
     2341 tcccattata cccctaactt tacggatgta ctattttgtt tgcatccttg tccatcttaa
     2401 aattttctaa tatcacctga gttggccaat gagcagtcct agaataaagc ccagaactgc
     2461 gaaaactagg ctggcatgga tcccctgaac tgaagcatgc cagaaggaat ggtcaataag
     2521 gtcccaaagc aaaaagacca gccccctaac acacccagta gtcatccagt gtaaatagca
     2581 tagggtaaat cctcctccaa tcgaactccc cacccacccc cttgaggggc agttactact
     2641 tttatttatg cttttttaag ggtaagcaaa tttgcttata aaacgagcca cacagtataa
     2701 tagggtgaaa agagatggtt tactgccata attgtgggac taaaaatgag gacgatgttg
     2761 agtactgttc taaatgtgga actccattga aggatgatgt ggaaagacgt catcaccaga
     2821 agcgccagag ggatgaatgt tttggccttc cctacggtaa ccttatcggc cccctgatca
     2881 tcggggttat tttgatactg gctggtcttt cttccatcta cggatttccg ttctggcagt
     2941 atctgtggcc agccctcatc gtactaatcg gcatattaat tatcgccggg gccatctaca
     3001 aggccacgca ccgggattaa gtcttgagaa ccaggataaa tctaaaacta aagaacatct
     3061 cttatctata aggacaagct ggaatagagc actgacggca tatcagatca tggtccctgg
     3121 ccgaaatggt ggtcctggtg tatatccaca gaccaataag caccagaaaa taggggctta
     3181 gattggcttt gaattccagg atccccacca ggatcatcac tgctccggtg accagcacca
     3241 ggttggataa aaacttaacc ggcgctctat ctatttttga atagttaagc tgcatcaaag
     3301 ctaaaaacac agccagcacc ccagcccaga ataggatggg gctgctgaac ccgtagaggt
     3361 aataaaaaag gcaccacagg atcgccaggg cacctcccag gaaaagaccg gaacaccctg
     3421 cgcagtacct tttcccttgg agggtgaagg tgtgggtttc aaattcacta cacaccgggt
     3481 ggtgtccgct tggctctgac ttttctcttt cccggtttag taaccgtaga cagtggtggg
     3541 ggtagccggc ggccatcatc cccagtacac agaccaggat gaacactgct aaaaccaggg
     3601 ttttgctggt cttggcagcc gggacccata ggtacatcaa aactaaaaac aagagtcctg
     3661 ccagggataa aaagctgagt aagcggttgc tattcaaaaa gacccctcaa tgattgtaac
     3721 aggccctgac cagccatgta acggtaggtc attcgtttaa actgattttt agaaaagact
     3781 ccagtgaggc gccttttcac actgccgtag aaatcccggt aacatagaaa cagctcctcc
     3841 tgtacttcat tacgggtcag atgctgggtg ggcatcaccg catggaccat atcgtagttg
     3901 ctccagttat agtcctccaa ccaaccattc cgctgggcgg tttcatagag ttctgtgcca
     3961 gggaagggag ttaatatcat gaagatggct atatcagggt ccacgtagtt gacaaactcc
     4021 cggaactctt ccatggactg gtgggagtcg ctccggtccc cagtgatgaa tgtggcctgg
     4081 gcgaatatat catggtcctt tagaagattc atggccagcc gggagtcctc agtcctgatc
     4141 cctttatggt aagagttgat ggtggctggg tcgtggcgtt ccagtcccgc cattacccag
     4201 taattaccag ccttccgcag ctcaggcagc acatcctggt tttttatgat ggcatcacta
     4261 cgggcctgca caaaccactt cacatcctcg gaaagtcccc tctttatgag ttcgtcacat
     4321 aactttcgag tgtgggggcc cagtcccagg ttgtcatcgg tgagccagtg gaagttgatg
     4381 ccctgttcct g
//
LOCUS       DAOQVW010000057         4390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_22257_length_4390_cov_3.323875, whole genome shotgun sequence.
ACCESSION   DAOQVW010000057 DAOQVW010000000
VERSION     DAOQVW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22257_length_4390_cov_3.323875"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKK85_02055"
     CDS             <1..194
                     /locus_tag="PKK85_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNS24836.1"
                     /translation="FSQLSELTGLKGGNLLFHLQKLTETDMILQRHERGDYMITEKGY
                     SVLMALSELNCILSRKNIS"
     gene            complement(212..883)
                     /locus_tag="PKK85_02060"
     CDS             complement(212..883)
                     /locus_tag="PKK85_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNS24837.1"
                     /translation="MNKEIARYLQKISVDSRFVSILEDRIVVNNLRYSRFSRAREEIF
                     YHKFPEVRVNRSKVFQRIATRASRNLKAELKPRDRVALFRDGDCVSQTLYAVLEPYTR
                     KYGIEIIQFELWGELEQLDVDKVALPFHLDCEVESLLEKMLSGDKISLESDRTSFNDH
                     KLIYPLINIPRDWILSWTGSEGIPCTEDGSGGMAPEMVQFLSSFIPDVREKMYKSAQF
                     LRENE"
     gene            1048..1977
                     /locus_tag="PKK85_02065"
     CDS             1048..1977
                     /locus_tag="PKK85_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24838.1"
                     /translation="MFKIFGGKKKQEEREVLKVDADGPVDCLCDFTFNFHWQHKGERL
                     DPTWLIPGTDITFGQVVEQLKNGGDVHIMADAGHRLCSSMGVDLQYFRGTGKDLPVGD
                     VYVEGDVDSRMGISMTRGSIYVKGKVKEPMGNLVEVKSSHKKYRQFRSITDIATNGLG
                     GDKLVGANLMGKKFIIQDGTVKDTLGARLDRDLEIILQGNVDLSTGILMRQGVVRVNG
                     NAGKNTGALLNGGTVVINGNTDDFTAIDMIKGHIIVNGDAGKFMAANKKSGYILARTG
                     SPIPPASEKKLESSNHKLLQTMGFRPQSFKKFE"
     gene            complement(1979..2797)
                     /locus_tag="PKK85_02070"
     CDS             complement(1979..2797)
                     /locus_tag="PKK85_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNS24839.1"
                     /translation="MGTADVIPGVSGGTMALITGIYQRLIHAISQINLTFLFYALEGE
                     WAKSRLELKKWDFNLFIPLLAGIGLAVLTMSKVMTMLLTVYTAPTYAFFFGLILASAG
                     FVYKHVDELNLKNLTFLVVGLVFAILFVGLNPIQANHTLPIIFLSGVVAICAMILPGI
                     SGAFILLLLNQYEYMLNALNELKIVEIIVFILGALIGILSFSRLLDYLLDHHKSITMA
                     FLVGLMIGTLRLPYSKISATMDSPWPVLIAAIIGFVLVVVLEKQFEKHHIPWEA"
     gene            2990..3607
                     /locus_tag="PKK85_02075"
     CDS             2990..3607
                     /locus_tag="PKK85_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNS24840.1"
                     /translation="MFSVVEYVSNLVIHLIESLGYWGVFLGMTIESACIPLPSEVIMP
                     FAGFVAWEGQLTLWGIILVGAFGNLVGSLVAYFVGWWGGRPFLEQYGKYVLISHRRLN
                     LAEEWFGKYGHEAVLISRMLPIIRTFISLPAGIAHMNLKKFILYTFLGSLPWCFALGY
                     LGFLLGPNWATLQEYWHYLDILVVVGAVAFLAYLLHRHHGKSHLE"
     gene            3685..>4390
                     /locus_tag="PKK85_02080"
     CDS             3685..>4390
                     /locus_tag="PKK85_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNS24841.1"
                     /translation="MRRFMLTLRLWLATIILFGILYAIIGIISTYFGISSPLFFAILA
                     LVVVLGQYLLGPKMVEAVMHVHYVSPQEAPQLHAMVEELAMNANIPTPKVGIAEVNIP
                     NAFAFGRSKKDGRVCVTRGVLNLLDEEELKAVLGHEISHIRHQDMVVMTLISVVPLIC
                     YYIFISTIFSKGDSDSGAGIIGLVALAGYILGNLLVLFISRVREYYADQGSVEIGGKP
                     HKLASALYKLVYGSANI"
BASE COUNT         1195 a         1001 c         1064 g         1130 t
ORIGIN      
        1 ccttctccca gctctctgaa ctcactggcc tgaagggggg aaatctactc ttccacctgc
       61 agaaactcac cgaaacagat atgatactgc aacgccatga gaggggagat tacatgataa
      121 cagaaaaggg ttacagtgtt ttaatggcac tttccgagtt gaactgcata ctatcacgta
      181 aaaacatctc ttaacatatg ttttaagatt tttactcatt ttcccttaga aattgtgcag
      241 atttgtacat cttctcacgc acatccggga tgaagctgga taggaattga accatctccg
      301 gggccatccc ccctgatcca tcttcggtgc agggaatacc ttcagaacct gtccaggaaa
      361 gtatccagtc ccgaggaatg tttataagtg gatatataag tttatgatca ttaaatgatg
      421 ttctgtcaga ctctaaacta attttatcac cactcaacat cttctccagg agagattcca
      481 cctcacagtc taagtgaaag ggcaaggcca ccttatccac atccaactgt tctagttccc
      541 cccataactc gaattggatg atttcaatac cgtacttccg agtgtagggc tctaaaactg
      601 catataaagt ctggctgaca cagtctccat ccctaaagag agccacccta tccctaggct
      661 tcaactcagc cttcaagtta cgggatgccc tggtagcaat gcgctgaaat actttagacc
      721 tattgaccct tacttctggg aatttatggt agaagatctc ttctctggcc cgggaaaatc
      781 gtgagtagcg caaattgttc accactatac ggtcctccag gatactgaca aaacgactat
      841 ccacactgat tttctgaaga tatctggcta tctctttatt cataaccttc accgcggagt
      901 tctggcctga gaaagggttt gatgaattgg aaatcagacc tttagatggt attgattcct
      961 ataggaaaag atgggggtgg ttggtgtcat atcattttaa ggatcagccc ctataagaat
     1021 agattaggta tcttaagggg tggtttattg ttcaagattt tcggtggcaa aaaaaagcag
     1081 gaagaaagag aagtcctaaa agtagatgct gatggcccgg tggactgtct gtgcgacttt
     1141 accttcaact ttcactggca acataagggg gaaaggctgg atcccacctg gcttatcccg
     1201 gggacagaca tcacctttgg ccaggtggtg gagcaattga aaaatggggg tgatgtgcat
     1261 ataatggctg atgctggtca tcgcctgtgc tccagtatgg gagtggatct ccagtacttc
     1321 cgagggaccg ggaaggacct cccggtgggg gatgtgtacg tggaggggga tgtggacagc
     1381 cgtatgggga tcagcatgac ccgtggatca atttacgtta agggaaaggt taaagaaccc
     1441 atgggtaatc ttgtggaggt taaatcttcc cataaaaagt atcggcagtt ccgttccatc
     1501 accgacattg ccaccaatgg cttaggaggt gataaactgg tgggagccaa tttaatgggt
     1561 aaaaaattta tcatccagga tggaacggtt aaagacaccc tgggcgcccg tctggatcgg
     1621 gatctggaga tcatcttaca gggcaacgtt gatctgtcca ccgggatact catgcgccag
     1681 ggggtggtgc gcgtcaatgg aaacgccggt aaaaacacgg gcgccctcct caatggaggg
     1741 acagtagtta tcaacggcaa caccgatgac ttcactgcca tagatatgat taaaggtcat
     1801 ataattgtta atggggatgc tggtaagttt atggcggcca acaagaagtc cggctatatt
     1861 ttagccagaa ccgggagtcc aatcccacct gccagtgaaa aaaaattgga aagctccaac
     1921 cataaactat tgcaaactat gggtttccgt ccccagagtt ttaagaaatt cgagtagttt
     1981 aggcttccca ggggatatgg tgcttttcaa attgtttctc cagaactaca accaggacaa
     2041 agccaattat tgccgcaatc aacactggcc atggagaatc catggtggcg ctgatcttac
     2101 tgtagggcag gcgcaaagtt ccaatcatca gaccaaccag gaatgccatg gtaatggact
     2161 tatggtgatc taacaggtag tccaggagcc gggaaaagga gagaatacct atcaaagccc
     2221 ccagtatgaa aacaattatc tccacaattt tcaactcatt cagggcattg agcatgtact
     2281 catattgatt taaaagtagt aagataaatg cccctgaaat accagggagg atcatggcac
     2341 agatagccac cacaccggaa agaaagatta ttggtaaagt gtggttggcc tgaatagggt
     2401 tcaggcccac aaagagtatg gcaaacacca gccccaccac caggaaggtg aggttcttca
     2461 agttcagttc atccacatgt ttgtaaacaa aaccagcaga agccagtata aggccaaaga
     2521 agaaggcgta ggtgggggct gtgtacaccg tgagcaacat ggtcatgacc ttggacatgg
     2581 ttaaaactgc caggccgata cctgccagta gtggtatgaa taagttgaaa tcccacttct
     2641 tcaactccaa acgggattta gcccactccc cctctaaggc ataaaatagg aaggtgaggt
     2701 tgatctggct aatggcatga ataagtctct gataaattcc ggtgataagg gccatggtgc
     2761 ctccggatac tccaggtatc acatcggcgg ttcccattag aagtcccctc agaaatatca
     2821 gcaaggtttc tctgtaaatc ctgatttttc gcatttttct aaccatccta taattaatgt
     2881 gaaattccca tgaagtaata ggccacagct aagaatcaag tcagcagata gtattaagta
     2941 gataaatcat aggagcttat ataactattt ctaaaggagt tttttttaca tgttcagtgt
     3001 tgtggagtac gttagtaacc tggtgatcca tctcatcgaa tcccttggtt actggggtgt
     3061 ttttttggga atgaccattg aaagtgcctg cataccactc cccagtgaag ttatcatgcc
     3121 tttcgccgga tttgtggcct gggagggaca gttgaccctg tggggcatca tcctggtggg
     3181 agccttcggg aacttggtgg gctctctggt tgcctacttc gtgggttggt ggggtgggag
     3241 gccttttctg gaacagtacg ggaagtacgt gctcatcagt caccgtagat taaacctggc
     3301 tgaggaatgg ttcggcaagt atgggcacga agcagtgctc atcagccgga tgctgccgat
     3361 tattcgtacc ttcatctctc tacccgccgg gatcgcccat atgaacctga agaaattcat
     3421 tctctacacc ttcctaggct ccctaccctg gtgctttgcc ctgggctatc ttggatttct
     3481 cttaggccct aactgggcca cactgcaaga gtactggcac tacctggaca tattagtggt
     3541 ggtgggggca gtggcctttc tggcttatct gcttcatcgc caccatggta aaagtcacct
     3601 ggagtgatat ccttttatac attagtttta tatatcaata caaccaacaa tatgccccat
     3661 accccatcga ggtgttccta aaatatgaga agattcatgc ttactttaag gctatggtta
     3721 gccaccatta tcctattcgg tatattgtac gctataatag gcataatcag tacctacttc
     3781 ggcatttcca gccccctatt cttcgccatc ctggccttgg tagtggtcct gggccagtac
     3841 ctcctggggc ccaagatggt ggaggcagtg atgcacgtgc actacgtatc ccctcaggaa
     3901 gcccctcagc ttcacgccat ggtggaggaa ctggccatga acgccaacat ccctacaccc
     3961 aaggtaggta tcgcagaagt gaacatcccc aacgcctttg cctttggaag aagcaaaaaa
     4021 gacggcaggg tctgtgtaac ccggggggtt ttaaatctac tggatgagga ggaacttaaa
     4081 gctgtgctgg gacacgagat cagccacatc cgccaccagg atatggtggt gatgaccctc
     4141 ataagtgtgg tgccccttat ctgctactac atattcatca gcaccatctt cagtaaagga
     4201 gacagtgaca gtggagccgg tataatcgga ttggtggccc tggcagggta cattctgggt
     4261 aacctcctgg tgttgttcat atcccgggtc cgggaatatt acgctgacca aggaagtgtg
     4321 gaaatcggag gaaaacccca taaacttgct agcgcactct ataaactggt gtacggaagt
     4381 gccaatattg
//
LOCUS       DAOQVW010000058         4387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_22281_length_4387_cov_3.156048, whole genome shotgun sequence.
ACCESSION   DAOQVW010000058 DAOQVW010000000
VERSION     DAOQVW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22281_length_4387_cov_3.156048"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(456..779)
                     /locus_tag="PKK85_02085"
     CDS             complement(456..779)
                     /locus_tag="PKK85_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNS24842.1"
                     /translation="MRIAIASTDKVNADHFGRARGFFVYQWEGNDPELVEYRETNINP
                     EEKHQWAEGLSKLGDCEVIIAAQAGMNAKYGIKQAGLKLVEDEGTVEEVLERFVKHEL
                     FMANI"
     gene            complement(810..1550)
                     /locus_tag="PKK85_02090"
     CDS             complement(810..1550)
                     /locus_tag="PKK85_02090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS24843.1"
                     /translation="MGEQELYKKYAGYYDLIYNWMDYPGEADFIKRAITHHKKSPGNE
                     LLDVACGTGGHAQYLQDDFQILGVDINLEMLELAREKLPRVNFFQSDMKKLDLGLKFD
                     VIICLFSAINYHTCLEELEKTFQRFYQHLHVGGVLLFDLGFCLENWEEGRMLVDAVVE
                     GDLQLARISQSRLQDGVFNANFVFLIKEDGKMDFEVDQHRIGVFSTIKIQDLLCEIGF
                     KCHIYNGYHEEGWNPDSDERPVFVCIKE"
     gene            complement(2187..2885)
                     /locus_tag="PKK85_02095"
     CDS             complement(2187..2885)
                     /locus_tag="PKK85_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HNS24844.1"
                     /translation="MSFAEFYQIFGQIVFLAGISILVLLSVTLILGRMLIKDDRLIFP
                     RLLLFTIDVFYGLFKKFAENVGVDDKIVDQIGVEVRNKVNEKIFNRIKPQDKIMVLPH
                     CLRNTDCEATLESSGLVCKDCNRCVIGVLKKKGEEMGYRVFIIPGSTFLKKIVEKNKF
                     KAVLGVACYQDLNLAMNKLSKFHCQGVPLLRDGCVNTKVDSRIVMEKMGVQFGDSKKM
                     NKSCTTETYPEKTL"
     gene            complement(2984..3448)
                     /locus_tag="PKK85_02100"
     CDS             complement(2984..3448)
                     /locus_tag="PKK85_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24845.1"
                     /translation="MLTELLLGPDEVLFLYGWSGVVGLAKFNRKGIIFMGTPDLEEIA
                     IFLGEEDLIAVSAFNKGPTVEKGIKSMIYLLREMEMPLLVLPDGHPTSHRLPLVVSCG
                     EMVRLDCQIQAGTHPEQDILCACNDLSGIEIMATACGVEINRNRIDWKKEKL"
     gene            3796..>4098
                     /locus_tag="PKK85_02105"
     CDS             3796..>4098
                     /locus_tag="PKK85_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt chelatase"
                     /protein_id="HNS24846.1"
                     /translation="MRILKNLTFPFSAIVGQEDVKKALILNAINPSIGGVLIKGDKGT
                     GKTTAVRALADLLPSIKVVKGCPFNCDPEDKDSLCDSCKSGDFEVEEKKMRVVELPL"
     assembly_gap    4099..4108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4109..>4387
                     /locus_tag="PKK85_02110"
     CDS             <4109..>4387
                     /locus_tag="PKK85_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS24847.1"
                     /translation="TEDRVVGSINLEKALREGIKALEPGILAEANRNILYVDEINLLD
                     DNLVDVLLDAAAYGMNVVEREGISFAHPSNFILVGTMNPAEGDLRLQL"
BASE COUNT         1261 a          961 c          786 g         1369 t
ORIGIN      
        1 tgacctttta ttaacctaaa aatagtagag aacccgccca ggtattaagc taaggttggt
       61 agattatact atttaaaata tgtggtgaat aggtggatca tctgcagatg gtaatggtga
      121 tgataatatg aattttcatt aatattccat caaagccccc tgttttacat tcttcaagtg
      181 aattcttaat ggccgaacca tcaacttcat agttccacta actcttaaag gctgccaatt
      241 ccatggattg ggtcgatgta cctgatcaca tatctaaaac caacggaaaa tcgctgtacc
      301 gggaacaagt cactactcta acttcatgtc catttctatg gaattcaacc cctagataac
      361 gattaaccag gtttttttat cttctacaac tacaaaaagc acttcttgac acatagctat
      421 cgatatgctt tatactggga acatcttgaa tttgtctaaa tattggccat aaacagttca
      481 tgcttaacga aacgctccaa cacctcttcc acagtacctt catcctccac taacttcaaa
      541 cctgcctgtt taataccata cttggcattc ataccagctt gggctgcgat tataacttca
      601 cagtctccta gtttgctcaa tccttctgcc cactggtgtt tttcttcagg gttgatgttg
      661 gtttcccgat actccactag ctctggatca tttccttccc actggtacac gaaaaatccc
      721 cgagcccttc caaagtgatc agcatttact ttgtcagtgg atgctattgc tattcgcata
      781 tcatctcctc ccatcattcc attatttgcc tattctttta tacatacgaa cacaggtctt
      841 tcatcagaat caggattcca tccttcttca tggtacccat tatagatatg acatttaaaa
      901 ccaatttcac acagcaagtc ctggatctta atggtggaga aaacaccaat ccggtgttgg
      961 tccacttcaa aatccatctt accatcctct tttattagaa atacgaagtt ggcgttgaaa
     1021 actccatcct gcagacgact ttgggatatg cgggccaact gcagatcccc ttccaccact
     1081 gcgtccacta acatgcggcc ttcttcccag ttttccaggc agaagcccaa gtcaaatagt
     1141 agaaccccac ctacgtgtag gtgttggtag aatctctgga aggttttctc cagttcctcc
     1201 aggcaggtat gataattgat ggcagaaaag aggcagatta tcacatcaaa tttcaggccc
     1261 agatccagtt ttttcatgtc ggactgaaaa aaattaaccc tgggaagctt ttccctggcc
     1321 aattccagca tttctagatt aatatctact cccaagattt gaaagtcatc ctgaaggtat
     1381 tgggcatggc ctcctgttcc gcaagccaca tccagaagtt cattacctgg tgatttttta
     1441 tgatgtgtta tggccctctt tataaaatca gcctcacctg gatagtccat ccagttgtaa
     1501 ataaggtcgt agtaaccagc atacttttta taaagctctt gttctcccat ctattactcc
     1561 ctaactagat tatttattaa acattttaaa ctatgttcaa atcttcttta tcaagttccc
     1621 cacattttca gacaagtgaa gggcggtttt aatacctctc tcattgagga tgcattctct
     1681 gggagtggat aaataccata ataacttcaa tcataggggt tccttcaaac actgttatta
     1741 tctgcttctt gagttaataa gcaatatttt ttacttatct attgttggtg taggtagtgt
     1801 tggatctata ccgaagggca ttcttgtgaa taaattaagt tctcaagcaa tacataactt
     1861 gtaattaaca gaaaagtctg gaaaaatgga aaaacaacat caaaaatact taatcattat
     1921 gtcctaatct ggataacctt tacataaatt ttttttatcc tgcatttagg ttatctagat
     1981 cctcatccac tccattgggg tttgatatct gattggttca tagtttcctg accatcggta
     2041 ttatattggg ccttattgtg gatgtactcg tttttaaaac gggagaactt accttcccct
     2101 tatatgacct ggttcataga atgtaatggc accttaagtt aggttctaag gcgaaaagtt
     2161 aaaaagaaga tttatgtgta aagattctac aaggtttttt cggggtaggt ttcggtggtg
     2221 cagcttttgt tcatcttttt ggaatcacca aactgtactc ccattttctc catcacgatc
     2281 ctactatcca ctttggtgtt aacgcaaccg tcccttagga ggggaacacc ttgacaatgg
     2341 aacttggaga gtttattcat agccagattc agatcctggt agcaagccac gcctaaaacg
     2401 gctttaaact tgtttttttc cacaattttt tttagaaaag ttgaacctgg aattataaac
     2461 actcgatatc ccatttcttc cccttttttt ttcaaaacac caataacgca gcgattacaa
     2521 tctttacata ccagacctga cgattccagt gtggcttcac aatcagtgtt ccggaggcag
     2581 tggggtaaca ccatgatttt atcctggggc ttgatcctat tgaatatttt ttcattaact
     2641 ttattcctga cttctacccc gatctgatcc actatcttat catccacacc cacattctct
     2701 gcaaacttct tgaaaagccc ataaaacaca tctatggtaa aaagaagtaa cctgggaaaa
     2761 attagacgat catctttaat taacattcga cccagtatca gggttacaga caataggacg
     2821 aggattgaga tacctgccag aaaaactatc tgtccaaata tctgataaaa ctcagcaaaa
     2881 gacatggtta atctcttaca atatactcgt atattaaact acttattaac aagttaaaat
     2941 taagtcatac tggatggata agagtaataa ttgctaggtt ttcttacagt ttttcttttt
     3001 tccaatcgat tctatttcgg ttaatttcaa caccgcaagc tgtggccatt atttctatgc
     3061 ctgataggtc gttgcaagcg cacagaatgt cctgttccgg atgagtaccg gcttgtatct
     3121 gacaatccag gcgcaccatt tctccacagc taaccaccag tggcagccgg tgtgatgtgg
     3181 gatggccatc aggaagcacc aggagaggca tttccatttc cctcaatagg tagatcatgg
     3241 atttgatgcc cttctctaca gtgggaccct tattgaatgc agaaacagca atcaaatctt
     3301 cttctcccaa aaaaatagcg atctcttcta ggtcgggagt tcccatgaat ataatgcctt
     3361 tgcggttaaa cttggctaga cccacaactc cgctccaccc atataaaaat aaaacctcat
     3421 caggtcctag gagtaactcg gtcaacatga ttttcttaga tttccactac attatcggac
     3481 tgacaagagg ggcaaattat tcttctcccc atacctttaa actcattttc acaatccaaa
     3541 caacgatatt tttttgtttt gagttttttc atcttaatat cggccattgg gccgcctaca
     3601 ctgcacattt tatcaccaat ctaccttatg tgttccctgt tatttatgat ttgttatcat
     3661 ctctatctcc atttctgata gaaatattgg tgatgaggtg gagttaccac aatattatat
     3721 tatgattgca tccatacata cattagttac ggagttaact ttaggaaaat aaatgactaa
     3781 acattctaaa aaatggtgag aatattgaaa aaccttactt ttccattttc agctattgtg
     3841 ggtcaggaag atgtaaaaaa agctcttata cttaatgcta taaatccttc aatcgggggg
     3901 gtcctgatta aaggagataa gggaacaggt aaaaccactg cagtacgtgc cctggccgac
     3961 cttctcccat ctatcaaggt ggttaaaggg tgtcctttta attgtgaccc tgaagataag
     4021 gattcattat gtgattcctg taaatccggg gatttcgagg tggaagagaa aaaaatgagg
     4081 gtggtagaac tacccctgnn nnnnnnnnca acagaagacc gggtggttgg ctccataaac
     4141 ctggaaaagg ctcttcggga ggggatcaag gctcttgaac caggaatact ggctgaagca
     4201 aatcgaaaca ttctctatgt ggatgaaatc aacctgctgg acgataactt ggtggatgta
     4261 cttctggatg ccgctgctta tggcatgaat gtggtggagc gggaaggaat ctcatttgct
     4321 cacccctcta atttcatact ggtggggacc atgaaccctg ccgagggaga tctccgacta
     4381 caactat
//
LOCUS       DAOQVW010000059         4377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_22351_length_4377_cov_3.227210, whole genome shotgun sequence.
ACCESSION   DAOQVW010000059 DAOQVW010000000
VERSION     DAOQVW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22351_length_4377_cov_3.227210"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="PKK85_02115"
     CDS             <1..149
                     /locus_tag="PKK85_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNS24848.1"
                     /translation="FSFISHIMTLNPGDVIATGTPPGVGQIQGGDVAEATVEDVGTLI
                     NQVQ"
     gene            complement(256..459)
                     /locus_tag="PKK85_02120"
     CDS             complement(256..459)
                     /locus_tag="PKK85_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24849.1"
                     /translation="MVLNNFMGIHGSEEIFLEVNTRYHKLVDYLLENKFRIKKSVNRM
                     LLTAFEGRHLEKSSHFVMRAWHA"
     gene            complement(542..832)
                     /locus_tag="PKK85_02125"
     CDS             complement(542..832)
                     /locus_tag="PKK85_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24850.1"
                     /translation="MRPDAGENIGLNYKMGFQSAYPSLILKVPESIVIEDNPDIRVKG
                     LWEMKPAHRKLILDNIDNWTQKKMSGLSFRHDLELAHSLGGEVLITLRKISP"
     gene            complement(822..1142)
                     /locus_tag="PKK85_02130"
     CDS             complement(822..1142)
                     /locus_tag="PKK85_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24851.1"
                     /translation="MHISPMREEHLEQMVELDLLAFNRKKPRLLESGGVNGHGPPWVV
                     MLFWIMNKYGGKVSPKLWVAKDTWGRWGFTLTFKARVWVKAHKKRIRVSEKTLPSHRP
                     GDAS"
     gene            complement(1164..4004)
                     /gene="leuS"
                     /locus_tag="PKK85_02135"
     CDS             complement(1164..4004)
                     /gene="leuS"
                     /locus_tag="PKK85_02135"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNS24852.1"
                     /translation="MEIKWQRKWQDAKLFHSNPDDREKLFLTVAYPYPSGAMHVGHGR
                     TYTVPDVYARFKRMQGYNVLFPMAWHVTGAPVIGIAKRIERQDPWTLSIYKNVHKVPD
                     EELQRFTDPHYIVEYFSSEYREVMTRLGYSIDWRREFKTLDPHYQKFISWQFRKLKEK
                     GLVRIGAHPVKYCPECLNPVGDHDLLEGEGVGINELTLVKFQYCGSYLVAATFRPETL
                     FGATNLWINPDEEYVRVKYQGEEWIISKKSFDNLMNQKEVEIVGEVDTEGMVGEYVEN
                     PLTREKLIILPASFVDPEYATGVVYSVPAHAPADYIALEDLKNDSVVLKKYQILDVVE
                     KIQPIGLIRLDGFGEHPAVEMVARMGIKNQQDPKLQEATNEMYKLEHARGVMDEHLTG
                     YAGMQVPEARDTVIRILLDSGKGDKMHEFAEKPVICRCGGKCVVKILEDQWFLKYSDP
                     NWKKTTLDCLHHMNAVPEEIRHNFEYFINWLHDWACSRRIGLGTRLPWDQQWLIEPLS
                     DSTIYMAYYAIATHLKKMNPEELDDAFFDGVFLDAEYSKTHQEFLDTKNEFNYWYPLD
                     WRLSAKDLVGNHLSFHLFHHSAIFPREKWPHGVVVFGMGLLEGHKMSSSKGNIVLLED
                     AIKTHGADVVRLFLMSSAEPWQDFDWREREVIGTKKRLEWFLEFADRVDQLHGSQIQL
                     KDHVKAPVVSQAINAWMISQVNQRVKDATLALDGFQTRKALQESLFLFKKDIDHYMHR
                     VDSSLTNPESRDEIAQVLAYVLGIWIRLLSPFTPHASEELWELHGGHGFASQAPWPEH
                     DPDLIDEVVQKGEEIVQGLADDIREIKKITKSQPQKIHIYLAPEWKWKVFEMAMKVGK
                     PDIGIIMRQVMQEDVHNDKKEISRFVQKLVREMTRIHYVGWVDEYRLISDSQDYLSQE
                     SGADVMVHQDAGYDPQKKARNALPYKPAIYLE"
     gene            complement(4067..>4377)
                     /gene="cutA"
                     /locus_tag="PKK85_02140"
     CDS             complement(4067..>4377)
                     /gene="cutA"
                     /locus_tag="PKK85_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNS24853.1"
                     /translation="ALIYITTSGEEESKKIAKTLLEERIVACANIVSSMKSFYWWEAE
                     IVEDDESILLLKTRKEKLDTLIRRIKDIHSYDVPCILEISIQNGSEDYLKWLDDSLQG
                     "
BASE COUNT         1066 a         1104 c          947 g         1260 t
ORIGIN      
        1 ttttcagctt catatcccac atcatgactt tgaaccctgg tgatgtcata gccaccggca
       61 ccccacctgg agtgggacag atacaagggg gtgatgttgc agaggccact gtagaggatg
      121 tggggaccct gattaatcaa gtgcaatgaa gttataatat aagttataac ttataagtaa
      181 tggggttata aaattactcc atgggcggtg gagggtcatg gtagccagtt ggtgggaaag
      241 attaacacct ctgagtcagg cgtgccaggc cctcataaca aaatgagaac ttttctcaag
      301 gtgccggccc tcaaaagctg ttaacagcat acggttaaca ctttttttta tccggaactt
      361 attctccagc agataatcaa ccagtttatg gtaacgggtg ttgacctcca ggaagatttc
      421 ctctgacccg tgaattccca taaaattatt taaaaccaac ccagcacctc attctggttt
      481 aatgagggtt tgataactcc ccatagatgt aaaaatacac tggtatgtga tgccattaca
      541 cctacgggct gattttccgt agtgtgatga gtacttctcc tcccagagag tgggccaact
      601 ccagatcatg gcggaagctt aatccagaca tcttcttctg ggtccaattg tcaatattat
      661 ccagaattaa tttccgatgg gcaggtttca tctcccataa cccttttact ctgatatctg
      721 gattatcctc aattacgatg ctctccggta ctttcaggat cagagatggg taagcagatt
      781 gaaatcccat tttatagttg agtccaatgt tttcaccagc atcaggacgc atctccaggc
      841 ctatgacttg gcagtgtctt ttcagatact ctaatccttt ttttatgagc ttttacccag
      901 accctggcct tgaaagtcag ggtgaacccc caccggcccc aagtatcctt cgctacccat
      961 aattttggtg aaactttacc cccgtacttg ttcattatcc agaacaacat gacaacccag
     1021 gggggtccat ggccattaac ccctccagat tctaaaagtc ggggtttttt ccgattaaag
     1081 gctaaaaggt caagttccac catctgttcc agatgttctt ccctcatggg gctgatatgc
     1141 accttatccc cccggaaatt atattattcc aagtagatgg ctggtttgta tggtaacgca
     1201 tttctggctt ttttctgtgg atcgtagcca gcatcctggt gcaccattac atcagcccct
     1261 gattcctggc tcagataatc ctgggagtcg ctgattagcc tgtactcatc cacccagccc
     1321 acatagtgga tacgggtcat ctcccggacc aatttctgaa caaaccgtga tatctctttt
     1381 ttatcgttat gtacatcttc ctgcatgacc tgacgcatta tgatgccgat atcgggtttg
     1441 cccaccttca tggccatttc gaagaccttc cacttccact ccggagccag gtagatgtgt
     1501 atcttctggg gctgggattt ggtgatcttt tttatctccc gtatatcatc agccagtccc
     1561 tgcacgatct cttcaccctt ctgcaccact tcatcaatca aatccgggtc atgttcgggc
     1621 cagggggcct gggatgcaaa tccatggcca ccatgcagtt cccacagttc ttccgatgcg
     1681 tggggtgtga agggagacag tagccgtatc cagatcccca gtacataggc cagtacctgg
     1741 gcaatttcat ctctggactc tggattagtt aacgaggaat ctactctgtg catgtaatgg
     1801 tctatatcct tcttgaaaag gaataaagat tcttgaaggg ccttcctggt ttggaatcca
     1861 tccagggcca gagtggcatc tttaaccctc tggttgactt ggcttatcat ccaggcgttg
     1921 atggcctggc taaccactgg ggctttcaca tgatccttga gttggatctg ggagccatgc
     1981 aactgatcca cccggtccgc gaattcaagg aaccattcca ggcgtttctt ggtgcctatc
     2041 acttcccttt ctctccagtc gaagtcttgc cagggctcgg cagaggacat caagaacaag
     2101 cgcaccacat ctgcgccatg ggtcttaatg gcatcttcca ggagtacaat gttaccctta
     2161 gaggaagaca tcttatgacc ttccaggagg cccataccaa agaccaccac cccatgtggc
     2221 catttttccc tgggaaaaat ggctgaatgg tggaataagt ggaaagaaag gtggttgcct
     2281 acaagatcct tggcggaaag tctccaatca aggggatacc agtagttgaa ctcatttttg
     2341 gtgtccagga attcctggtg tgttttagag tattctgcat ctaggaaaac tccgtcgaag
     2401 aaggcatcat ccagttcttc agggttcatc ttcttcaaat gggtggcaat ggcgtagtag
     2461 gccatgtaaa tggtagaatc acttaaaggt tcgatgagcc actgttgatc ccagggtaaa
     2521 cgggttccca accctatacg gcgggagcaa gcccaatcat gaagccaatt gatgaaatac
     2581 tcgaagttgt gtcggatttc ctccggaacg gcgttcatgt ggtggagaca atccagggtg
     2641 gtttttttcc aattcgggtc ggagtacttg aggaaccact ggtcttccag gatcttgacc
     2701 acacatttac ctccgcagcg gcatatcact ggtttttccg cgaactcatg catcttatcc
     2761 cccttcccag aatcaagaag tatccttatg accgtgtccc tggcttcggg gacttgcatt
     2821 cctgcgtaac cagttaggtg ttcatccata acacccctgg catgttccag tttgtacatc
     2881 tcattggtgg cctcctggag cttaggatcc tgctggtttt taattcccat cctggccacc
     2941 atttccacag ctgggtgttc accaaagcca tccagcctaa taaggccgat gggctggatt
     3001 ttctccacta catccaggat ttgatatttt ttaagaacta ccgagtcgtt tttaaggtcc
     3061 tccagggcaa tgtaatctgc cggagcgtga gcaggtaccg aataaaccac ccccgtggcg
     3121 tattctgggt ctacaaatga agcagggagt attatgagtt tttcccgggt taatggattt
     3181 tccacgtact ctcccaccat cccctctgtg tccacttctc ccactatttc cacttctttc
     3241 tggttcatca agttatcgaa ggatttttta cttataatcc actcctcgcc ctggtatttc
     3301 accctaacat actcttcatc tgggtttatc cagaggttgg ttgctccgaa gagggtttcc
     3361 gggcggaaag tagcggccac aagataggac ccgcagtact ggaatttcac cagggttagt
     3421 tcgttgatac ccaccccctc cccttccagg aggtcatgat cccctactgg attcagacat
     3481 tcaggacaat acttcacggg gtgggctcca atacgaacta gtcccttttc tttcagtttc
     3541 cgaaactgcc aactgatgaa cttctggtag tgggggtcca aggttttgaa ctcccttctc
     3601 cagtctatgg agtagcccaa ccgggtcata acctcacggt attcactgct aaagtactcc
     3661 actatgtaat gaggatcagt gaaccgttgc aattcctcat caggcacttt atgtacgttt
     3721 ttatagatac tcagagtcca tgggtcctgt ctctctatcc gcttggcaat accaattact
     3781 ggtgccccgg taacatgcca ggccattggg aagaggacgt tgtagccctg cattcgtttg
     3841 aacctggcgt aaacatctgg taccgtgtag gttcttccat ggccaacatg catagcccca
     3901 ctagggtagg gataggccac ggttaaaaac agtttctccc ggtcatcagg attggagtga
     3961 aacaacttcg catcctgcca cttcctctgc cacttaattt ccatattgat agctgtcaat
     4021 caatcaccgc cataaaaatt aacaataaat aactgttaca ctaattttat ccctgcaatg
     4081 aatcatctaa ccatttcagg taatcttcgg aaccattctg gatggatatt tccaggatac
     4141 agggaacatc gtaactatgt atatccttaa tccgccttat cagggtatcc agtttttctt
     4201 tcctggtttt caggaggagt atggattcat catcttccac aatttcagct tcccaccaat
     4261 aaaatgattt catagaagat acaatgttag cacaagccac aattctctct tccaggagtg
     4321 ttttagctat tttttttgat tcctcctctc cggaggtggt tatataaatc aatgcat
//
LOCUS       DAOQVW010000060         4374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_22371_length_4374_cov_3.317203, whole genome shotgun sequence.
ACCESSION   DAOQVW010000060 DAOQVW010000000
VERSION     DAOQVW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22371_length_4374_cov_3.317203"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="PKK85_02145"
     CDS             complement(<1..176)
                     /locus_tag="PKK85_02145"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515622.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNS24854.1"
                     /translation="MNITEKILARAASQKEVQPGEIIEAQVDLAMSHDGTSPPTIKTF
                     RQMAQRVWDPDRIV"
     gene            complement(204..1379)
                     /locus_tag="PKK85_02150"
     CDS             complement(204..1379)
                     /locus_tag="PKK85_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HNS24855.1"
                     /translation="MRYMVSPFNQQVDLKFPEKITVYDTTLRDGEQTPGVCLRTPEKL
                     QIARKLDELGVHQIEAGFPIVSNEERRSISAIVKENLNADILALSRTRKEDIDMAIDC
                     DVDGIITFMGTSDLHLKHKFKKTKEEILNICISSIEHAKDHGLFVAFSAEDATRTDIN
                     FLKEIYKKAEDYGVDRVHIADTVGAISPHAMDYMVGELKKDLKTEISLHCHNDFGMAL
                     SNSISGLLAGASAVSTTVNGIGERAGNTSLEELVMTLLLIYGVDLKFNISIFYELSRL
                     VEKLTNMPIPNNKPIVGRNVFRHESGIHVDAVIEEPLTYEPFLPELIGHHRRIVLGKH
                     SGCRAVKAKLTECGIEVSRDELCKIVEKVKEKREEGKYINDDVFNEIIRSIRGPVDI"
     gene            complement(1636..2175)
                     /gene="cyaB"
                     /locus_tag="PKK85_02155"
     CDS             complement(1636..2175)
                     /gene="cyaB"
                     /locus_tag="PKK85_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNS24856.1"
                     /translation="MMEVEVKAHAPDLSDVARKLGDIGAQWIKEEYQEDIYFNAPHKD
                     FAKTDEALRVRKITDDVSKNTFITYKGAKLDELSKTRVEVEMMVEDPLKAVNIFENLG
                     FRAVATVKKYRTIYYLDDLIITLDRVKDVGNFVEIEQDTEEGEDYQEVLNHIFAVYKK
                     LGIKEGFERRSYLEILGIH"
     gene            complement(2206..2610)
                     /locus_tag="PKK85_02160"
     CDS             complement(2206..2610)
                     /locus_tag="PKK85_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24857.1"
                     /translation="MVSPRRYREQLENLEIEGLEIDVSSIRNAMEVLNSLEEYEKVLR
                     QIRYNVRSDVRNIRMDYLHQLQKIESSNKKGLFGREKSKETILKEKKSLKKERDLEIA
                     AYDIVENMIDNYLAQIEDARDYIRHSIEKRVG"
     gene            3043..3588
                     /locus_tag="PKK85_02165"
     CDS             3043..3588
                     /locus_tag="PKK85_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HNS24858.1"
                     /translation="MSDVEIKVENIVASATLGKSIDLPQVAPALEGVEYNLEQFPGLV
                     YKLKEPKTAALIFGSGKLVCTGAKSIEDSKKAIHIAVDKMRTLDPDIPTEFEIKVQNI
                     VASANLMKTLNLEAVALDLENTEYEPEQFPGLVYRLGEPKVVLLLFGSGKVVCTGAKT
                     ISDAQLGVEKTKERLTELDLL"
     gene            3622..>4374
                     /gene="serB"
                     /locus_tag="PKK85_02170"
     CDS             3622..>4374
                     /gene="serB"
                     /locus_tag="PKK85_02170"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNS24859.1"
                     /translation="MIKLIAFDLDNVLIDGEAIDEIAKLAGTESEVSKITQQAMEGKL
                     DFETALKQRVALLKGTSVEEINRIVQKIPIMEGAEETIAELKNRGYKIATISGSFENI
                     AQRLKDALDLDYTFSNTLEEDEGFLTGEVSGPLVVGSKGDVLAKIMDIEKLSSEECAA
                     VGDGANDISMLEKAGLGVAFNAKPVLKQSADIIVEKKDLRELLNVFDEKPVEGNLEIT
                     LDSKKSFNELLDEKRDFEKRLREITTVRDNLNE"
BASE COUNT         1338 a          844 c          775 g         1417 t
ORIGIN      
        1 attacgatcc gatccggatc ccaaaccctt tgggccattt gacggaatgt tttgatagtg
       61 ggcggagaag ttccgtcatg gctcatggcc agatctactt gggcttcgat gatttctcca
      121 ggctgtactt ctttttggga agcagctcta gccagtatct tctctgtgat attcataaag
      181 attaaaaact ccagtaaatt tctttaaata tcaactggtc ccctgatcga ccgaatgatt
      241 tcattgaata catcgtcgtt gatatattta ccttcttctc ttttttcttt cactttttcc
      301 acgattttac aaagttcatc ccgacttact tcgataccac attctgtgag cttggccttt
      361 acggctcgac atcctgaatg ttttccaagg acaatacgtc gatgatgacc tatcaattct
      421 ggaagaaagg gttcataagt taaaggttcc tcaataacag cgtctacatg tattcctgat
      481 tcgtgtctga acacatttcg tcccacgata ggtttattat tcgggatggg catgttggtc
      541 aatttttcca ccaatcgtga gagttcatag aaaatactaa tattaaactt aagatcaaca
      601 ccataaatga gtaatagagt cattaccaac tcttctaagg aagtgttgcc ggcacgttct
      661 ccaataccat ttactgtggt ggaaacagca cttgctccag ccaaaagacc agatatagaa
      721 ttggataatg ccattccaaa gtcattatgg cagtggaggg atatttctgt ttttaaatct
      781 tttttgagtt cccccaccat gtaatccatg gcatgaggac ttatagcccc tacggtatcg
      841 gcaatatgga ctcggtccac accataatcc tctgctttct tatatatctc ctttaaaaaa
      901 ttgatatcag tacgggtagc atcttctgcg gagaatgcta caaaaagccc atgatccttg
      961 gcgtgttcaa ttgaactaat acagatattc aggatttcct cctttgtctt tttaaatttg
     1021 tgtttaaggt gtaaatccga ggtccccatg aaggtgatga ttccatccac atcacaatca
     1081 atggccatat caatgtcttc cttacgggtt cgggacaggg ctaatatgtc tgcatttaaa
     1141 ttttctttga ctatggcaga tattgaacgt ctttcttcat ttgaaacaat gggaaacccc
     1201 gcttctatct gatgaactcc taactcatcc aattttctgg ctatttggag cttttcagga
     1261 gttctgagac agacccctgg tgtttgttcc ccatcgcgaa gtgtggtatc gtagactgta
     1321 attttttctg gaaacttcaa atccacctgc tggttaaagg gacttaccat atatcgcaag
     1381 agtattcaca acctgatttt tttgaaaaca tacaataaat gcactaattt cggtataata
     1441 aacatttaat tctattttag taatctgaaa gtatgatcaa aagattaaat ggagttaaca
     1501 atctgaagct aatcttttag tctataatca tttttatata aatgtaactt ccatttattt
     1561 atatactcat cctttaacaa tccaccagcc gctaacttcc ttccggaagc tttcaccaaa
     1621 tccttactgt acattttagt gtatgcccaa tatctccaag taggatcttc tttcaaaacc
     1681 ttcctttatt cccaattttt tgtaaaccgc aaagatgtgg ttcaacactt cctgataatc
     1741 ctcaccttct tcggtgtctt gttctatttc cacaaaattc ccaacatctt tgaccctatc
     1801 cagagttata ataaggtcat caagataata aatggtcctg tactttttta cggtggctac
     1861 cgccctgaaa cctagatttt caaaaatatt tactgcttta agtggatctt ccaccatcat
     1921 ctcaacttca acccgggttt tactcagttc atccagttta gcacctttat aggttataaa
     1981 agtattttta gatacatcat cagtgatttt tctaaccctg agggcttcat cagtcttagc
     2041 aaaatcttta tgaggagcgt taaaataaat atcttcctgg tattcttctt taatccattg
     2101 tgcaccaata tcccctaatt ttcgggcaac atccgaaaga tcaggtgcat gggccttaac
     2161 ttccacttcc atcatgttca cactgtccaa atctttaatt gacagttatc ctaccctttt
     2221 ttcaatagaa tgacgaatat aatcacgtgc gtcttctatc tgtgccagat agttgtcgat
     2281 catattttca acgatatcgt aggcagcgat ctccagatca cgctcttttt ttaaggactt
     2341 tttttctttt aaaatagttt ctttagattt ttcacgcccg aataatcctt ttttgttaga
     2401 actctctatt ttttgaagtt ggtgcaggta atccattctt atatttctaa catcactacg
     2461 aacattatat ctaatttgtc gaagtacctt ttcatactct tctaagctgt taagcacctc
     2521 catagcattc ctaatggatg atacatctat ttcaagccct tcaatttcta gattttccaa
     2581 ttgctcccgg taccttctgg gactgaccat aacaacatct cccttaagaa aaacttcaac
     2641 taatgaaaat attcaatatt tttatatgtc cctggtattt ataagtagtt tggcctcttt
     2701 tttatgaata atgtatagtt ttcctattaa aagacaaaaa gcggttattt atattattta
     2761 gcttttaaaa gatgaaatta aaaaaaaata agaatattga gctttctgtg atcatcctag
     2821 tttgggtaat tgatgtgtat atcgcattgg ttcaataaaa gaatagccac tttttataag
     2881 taaggagtac catctattat tagcatgtcc ttttttgtag agtgtgtgat taatatctgt
     2941 cactttttac aagcaagtca cacttttaaa tagtatataa aaaaccattt ataaaacata
     3001 aaagtacatt gttaatgact atgcactgga ggattaatta agatgtctga tgtggagatc
     3061 aaagtggaaa atattgtggc atctgccact ttaggaaaat ccatagacct tccccaagtt
     3121 gctccggctt tggaaggtgt ggaatataat ttagaacaat ttcctgggct tgtttataaa
     3181 ttaaaagaac caaaaacagc agcacttatt tttggatcgg gcaaactggt ttgtactggc
     3241 gctaagtcca ttgaagactc gaaaaaggcc attcacattg ctgtggataa aatgcgaacc
     3301 ctggatccag acatacccac tgaatttgaa ataaaagtcc aaaacatcgt ggcatccgcc
     3361 aacttgatga aaacattaaa cttggaagca gtggcccttg acttggagaa cacagaatac
     3421 gaaccagaac aatttccagg ccttgtttat cggttgggag aacctaaagt cgttctatta
     3481 ttatttggtt ctggtaaagt tgtttgtact ggtgcaaaga ccatcagcga cgcgcagtta
     3541 ggtgttgaaa aaaccaaaga acgattaacc gaactagatt tactttaatt atcatcctct
     3601 aatgaactaa ttggtgatgt tttgattaag ctcatagcat ttgatctcga taacgtactg
     3661 atagatggag aagccataga tgaaatagcc aaactagccg gcactgaatc tgaggtttca
     3721 aaaattaccc aacaagccat ggaaggtaaa ttagattttg aaactgccct aaagcaaagg
     3781 gtagccttat tgaagggtac ttctgttgaa gaaattaaca gaatagtaca aaaaataccc
     3841 ataatggaag gggctgaaga aaccatagct gaactaaaaa atcgaggtta taagattgcc
     3901 actataagtg gtagttttga aaatatcgcc cagcgattaa aagacgcgct tgatctagat
     3961 tataccttct ccaacacttt agaagaagat gagggatttt taacaggaga agttagtggt
     4021 cccctggtgg taggttctaa aggagatgtg cttgctaaaa taatggacat agaaaaacta
     4081 tcttctgaag agtgcgctgc ggtcggtgac ggtgccaatg atatttcaat gcttgaaaag
     4141 gcaggtttag gtgtggcatt taatgcaaaa cctgtgctaa agcaaagtgc cgacattata
     4201 gtagaaaaaa aggatttgag agaattgttg aatgtatttg atgaaaaacc tgtggaagga
     4261 aatttggaaa taactttaga ctccaaaaaa agttttaatg agcttctgga cgagaaaaga
     4321 gactttgaaa agagattaag agaaataacc actgtaaggg ataatctcaa tgaa
//
LOCUS       DAOQVW010000061         4354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_22518_length_4354_cov_4.331938, whole genome shotgun sequence.
ACCESSION   DAOQVW010000061 DAOQVW010000000
VERSION     DAOQVW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22518_length_4354_cov_4.331938"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PKK85_02175"
     CDS             complement(<1..641)
                     /locus_tag="PKK85_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01210 family radical SAM protein"
                     /protein_id="HNS24860.1"
                     /translation="MMGINKLNRELRERSLGRIDKKSPRKLAASWSSEDLLYSGPGRS
                     IFMVLPTPGCAWALSDSGGCTMCSYVADSPLETVTAEELLGIFHEHLKKQIQREALES
                     PTAVKLFVSGSFLNPEEVPVKARQGILMALDDYPEIEEVVVESRPEYVTREVLRECCE
                     ALPGKIFEVSMGLETWDERRRLNIINKGFSNEEFERAVSIIQELKSEYPVRSKV"
     gene            759..1646
                     /locus_tag="PKK85_02180"
     CDS             759..1646
                     /locus_tag="PKK85_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS24861.1"
                     /translation="MVRKIKVKSVLNKQKHVDNWFLTGYSLNPYSGCSFNCVYCYTRG
                     SKYGEHQVPGLAVKINGPEVLVRQLKNRARKKEYAFIALGSATDPYLPVEEEYKITQE
                     FLRIILRFHFPVHVLTRSPLILRDLDLLRKIGEKAIISRELQDRLDKGVIISFSFSTM
                     DPELASIFEPGAPEPELRLETMRKCKEAGFMVGAVFMPLLPYLTDTEESLDYMIGRVK
                     NQGADFVLAAALTLFGDGPADCRTRYYQALQEHFPEKVPETRALFGKSFAPSWKYQRE
                     LYLRVKNLSKNHKIRNSVI"
     gene            complement(1675..2640)
                     /gene="mer"
                     /locus_tag="PKK85_02185"
     CDS             complement(1675..2640)
                     /gene="mer"
                     /locus_tag="PKK85_02185"
                     /EC_number="1.5.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018537 - coenzyme F420-dependent
                     N5,N10-methenyltetrahydromethanopterin reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HNS24862.1"
                     /translation="MKFGIEFVPNEPIEKIVKLVKLAEDVGFEYTWITDHYNNKNVYE
                     TLALIAAGTETIKLGPGVTNPYVRSPAITASAVATLDEISNGRATLGIGPGDKATFDA
                     LGIEWTKPVSTIKDAIAMMSTLMAGEKTETGAQLGGVKSVQEKIPIYMGAQGPMMLKT
                     AGGFSDGALINASNPKDFEAAVPLIKEGAEAEGKSISDVDVAAYTCCSIDDDAGKALG
                     AAKIVVAFIAAGSPPPVFERHGLAPDTGAKFGEMLGKGDFGGAIGAVDDALMEAFSVV
                     GTPADFVPKIEALGEMGVTQYVAGSPIGPDKEKSIKLLGEVIDSF"
     gene            3352..>4354
                     /locus_tag="PKK85_02190"
     rRNA            3352..>4354
                     /locus_tag="PKK85_02190"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
BASE COUNT         1112 a          982 c         1076 g         1184 t
ORIGIN      
        1 accttggatc ttactggata ttcagattta agttcctgaa ttatggacac agcccgttca
       61 aactcttcat tggaaaaacc tttattgatt atgttcaacc ggcgcctttc atcccaggtt
      121 tccaagccca tggatacctc aaatatttta cccggcaaag cctcacaaca ctcccggaga
      181 acttctctgg tgacgtactc tggacgggat tccaccacta cctcctctat ttcgggataa
      241 tcatccaggg ccatgagaat tccctgcctg gcctttactg gaacttcttc tgggttgagg
      301 aagctgcctg atacgaataa cttcacggca gttggacttt ccagtgcctc tctttgtatc
      361 tgcttcttaa ggtgttcatg aaatattccc agcagctcct ctgcagtcac ggtctcaagg
      421 ggtgaatctg ccacgtaact gcacatggta cacccaccag aatcagaaag agcccaggca
      481 caaccagggg tgggcagtac catgaatatg cttcgaccag ggccggagta aagcaggtcc
      541 tcgctggacc agctggctgc cagttttcgc ggtgattttt tatcgattct tcccagtgat
      601 ctttctctca gttcccggtt taacttgttt attcccatca tttaaccttc caatgaaata
      661 tcaggatcat aatagtaatc tgaagatatt atatattaaa tggataatct aagtctttct
      721 aacacttttt ggataaaaga cataactgtg gtgaaaaggt ggtccggaaa attaaggtta
      781 aatccgtcct gaacaagcag aaacatgttg ataactggtt tttgactggt tactccctca
      841 acccctattc agggtgttca tttaactgcg tgtactgtta cacccgtgga agtaaatacg
      901 gggagcatca ggtgccaggg ctggcagtta aaatcaatgg ccccgaggta ctggttagac
      961 agttgaaaaa ccgggcccgg aaaaaagagt atgcttttat agccctgggt tcagccactg
     1021 atccttacct tccggtggag gaggagtata aaatcaccca ggagtttctc aggatcatcc
     1081 tccgcttcca ctttcctgtt catgttctaa cccgatcacc actgatatta cgggatctgg
     1141 acctgttacg taagattgga gaaaaggcca ttatctccag ggaacttcag gaccgactgg
     1201 ataagggggt gatcatctcc ttttccttct ccaccatgga tccggagctg gccagcatat
     1261 tcgaacccgg ggcccctgaa ccagaactaa ggttggaaac catgagaaaa tgtaaggagg
     1321 ccgggttcat ggtgggggcg gtgttcatgc ccctgttacc ctaccttact gacaccgagg
     1381 aatctctgga ttatatgatt ggccgggtaa agaatcaggg ggctgatttt gtcctggctg
     1441 ctgctctgac cctgtttggt gatggccctg ctgattgcag gacccgttat taccaggcat
     1501 tgcaggaaca ttttccagag aaagtaccag agacccgggc cctcttcgga aagtcctttg
     1561 caccttcctg gaaatatcag cgagagcttt acctcagggt gaagaatttg tccaagaacc
     1621 acaaaatcag aaacagtgta atttgaaaaa taaaaaagga aagaagaagt ttatttagaa
     1681 gctgtctata acttctccta atagtttgat ggatttttct ttgtcaggac cgatagggga
     1741 accggctacg tactgggtca cacccatttc accgagtgct tcgatttttg gcacgaagtc
     1801 ggctggggtc ccgaccacag agaaggcttc cattagggcg tcgtccacgg ctccgatggc
     1861 tccgccgaag tcacctttac ctaacatttc accgaattta gctccagtgt caggggccag
     1921 gccgtgtctt tcaaatactg gtggtgggga tccggctgcg atgaaggcaa ccacgatttt
     1981 tgcggcgcct agtgctttac cggcgtcatc atcaatggaa cagcaagtgt aagctgctac
     2041 gtcaacgtcg gaaattgatt taccttctgc ttctgctcct tcttttatga gtggaacagc
     2101 tgcttcaaag tcttttgggt tggatgcgtt aattaatgca ccgtcagaga atcctccggc
     2161 ggttttgagc atcattggtc cttgggctcc catgtagatt gggatttttt cctggacaga
     2221 tttaactcca cctaactggg ctccggtttc ggttttttca ccagccatta aggtgctcat
     2281 catggcgatg gcgtctttga tggtggatac tggtttggtc cattcaattc ctaaggcgtc
     2341 gaaggttgct ttgtcaccag ggccaatacc tagggtggct cttccattgg atatttcgtc
     2401 tagggtggca actgcagatg cagtaatggc agggcttcgc acgtaggggt tggttacacc
     2461 aggaccgagt ttaatagttt cagttcctgc tgcaattaaa gccagggttt cgtacacgtt
     2521 tttgttgttg tagtggtcgg taatccaagt gtattcaaaa ccgacgtctt ctgccagctt
     2581 tactagcttg acaatctttt ctataggttc atttgggaca aattcaatac caaacttcat
     2641 gattaatcac cagtcaataa tctatttggt acataaaaat attttgtgat ttaaccctaa
     2701 tcgaaagatt taaatagggc actatctttt aatataagcc catagtatta atagaagcta
     2761 ttaatggtct ttttaatggt tttattttct taagactggt ttaaaattga ttatgatgcc
     2821 taaaatatct atttaagtta ttttcaactt gaaccatgaa cttatcttat tctttcaaaa
     2881 aaatcttaat ctgaagggtt atcacccaac catccaagtc cactccatga aatggtcgta
     2941 gttggatcct gctggttctt tgatataata tatataatgg tagatgggca gtgaaatcat
     3001 agaaaacttt atatatcatc atcaactaag gctgttctgt ctctcaccca tggtgcctca
     3061 tcagcaccta gtttgcaata atttcttaaa actttgtaaa actacagggt gggtgtatag
     3121 tatgtgccac cattaaaagg cgtggaccgg agtgtgagaa catcactcta tgaaggttcc
     3181 aattccaatg gtgtctatag atatgagggc ctaaaccctt gcgaaagcaa acgtttaggt
     3241 ttgaggttgg tctgatagat gtggtgatat ttggttgcta ctaaattttc cttacttttc
     3301 caaattccca acataaacaa taaccaatat ggtataatcc gccttcaaac aaatccgttt
     3361 gatcctggcg gaggccactg ctattggggt tcgattaagc catgcaagtc gaacgatctt
     3421 cggatcgtgg caaacggctc agtaacacgt ggataaccta cccttaggac tgggataacc
     3481 ccgggaaact ggggacaata ccggatatgt ggagatgcct ggaatggtac tccattgaaa
     3541 gctccggcgc ctaaggatgg atctgcggca gattaggttg ttggtggggt aatggcccac
     3601 caagcctgtg atctgtacgg gttgtgagag caagagcccg gagatggaac ctgagacaag
     3661 gttccaggcc ctacggggcg cagcaggcgc gaaacctccg caatgcgagc aatcgcgacg
     3721 gggggacccc aagtgctact cttaacgggg tagcttttct aaagtgtaaa aagcttcagg
     3781 aataagggct gggcaagacc ggtgccagcc gccgcggtaa caccggcagc tcaagtggtg
     3841 gccgctttta ttgggcctaa agcgttcgta gccggcttga taagtctctg gtgaaatccc
     3901 gcagcttaac tgtgggaatt gctggagata ctattaggct tgaggccggg agaggctgga
     3961 ggaactccca gggtaggggt gaaatcctat aatcctggga ggaccacctg tggcgaaggc
     4021 gtccagctgg aacggacctg acggtgagta acgaaagcca ggggcgcgaa ccggattaga
     4081 tacccgggta gtcctggccg taaacgatgt ggacttggtg ttggaatggc tccgagctgc
     4141 tccagtgccg aagggaagct gttaagtcca ccgcctggga agtacggtcg caagactgaa
     4201 acttaaagga attggcgggg gagcaccaca acgcgtggag cctgcggttt aattggattc
     4261 aacgccggac atctcaccag gggcgacagc agaatgatgg ccaggttgat gaccttgctt
     4321 gacaagctga gaggaggtgc atggccgccg tcag
//
LOCUS       DAOQVW010000062         4317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_22775_length_4317_cov_3.311825, whole genome shotgun sequence.
ACCESSION   DAOQVW010000062 DAOQVW010000000
VERSION     DAOQVW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22775_length_4317_cov_3.311825"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="PKK85_02195"
     CDS             complement(<1..1803)
                     /locus_tag="PKK85_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS24863.1"
                     /translation="MDRIRILMLEDVPFDAELAEKELERGGIPFTTCRVENEEEFLRG
                     IREFQPHIILADHSLPSFDGKSALKIAKQQIPQVPFIFFSGKIGGEFVVEVLKEGAKD
                     YVFKNNISKLPYTIRRALDEVEEARELDRAARSLENREAQLRLITRNIQDMVMQVDVH
                     SQIQYLSSSLHTILGYHHQDFLGQPLNRFLELVHPDDQGKLTRALEGVKSSSTSVQEE
                     FRCKNAEGGYHWLESLINPFLDENQEENGLLLVIRDVDHRKKVELQLARHRENLEKVV
                     EERTRELEEANVKLSQEIMEREKLYKSLLNNEKRLESIINGSPIPTFAIDTDHEVVFW
                     NQALENFTGIKRRDVLGTTHHWQVFYPEERPCMADLLLENDWEGICKWYSTPPVSMEV
                     AEMVYSATGFFPQIRDGKWLRMTAFPLLDSEGEVIGAVETLEDITPEVEYQEKIKSSL
                     EEKEILLREVHHRVKNNLQIISSLISLQSNRLDDEETIHLFRKTINRIQSMALVHEKL
                     YQSHDFARINLAEYIKDLVQNVLNSYEGSQERIILEMDCEPVWININTAIPCALIINE
                     LVTNSVKHAFPHEGTGKIPLLAQLDDDLTGSFMGE"
     gene            2059..3054
                     /locus_tag="PKK85_02200"
     CDS             2059..3054
                     /locus_tag="PKK85_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNS24864.1"
                     /translation="MEDRYRNTRTALDSMTSTVPRVQPQDKVASIKQLLSTQACQLGN
                     IDYIYVVNRQGHLDGVLSIKEIFCSLDSEVTAREIMKQDLVVASPSTHQERVVYLALS
                     HGIKAVPVVDEDGLFQGVVPYDQILRIFNHEVQSDVFKFGGIFHKVGKEYTSIQSSTR
                     MMIRSRLPWLVVGVLGGTMAAGLIGYFENLLSEYLVLAFFIPVMVYMSDAAGTQSEAL
                     IIRSLALDPKLAMKSYFIRELKVAGVIGLVSGVLAAFTSLLGWTQPVLSLIVGFSMFI
                     SIISSVAIATVAPLIFYKLKYDPAVATGPLATIVSDITTLAIYFTTAMALTGLIH"
     gene            3163..3828
                     /locus_tag="PKK85_02205"
     CDS             3163..3828
                     /locus_tag="PKK85_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24865.1"
                     /translation="MMKQKLYLLWGETAPWGLVDDYQSRLEVICRVVNSRYGKHLHTP
                     LSPLQDPWKLGAVLFNISRVYDILDIVLKGLYPQSMHLVLVYDYLDELKEKDINHLDG
                     PALEKATQMMSKLERNGLLFEMKVDKKETDLALSGLINSVQLLKKGLSAKKRRIISEY
                     VKTGKQSLAAENLNMTQQGVSKALKSSNWREIKDGEQKLQRALEIYSDRLGRKQRSLD
                     DFY"
     gene            4023..>4317
                     /locus_tag="PKK85_02210"
     CDS             4023..>4317
                     /locus_tag="PKK85_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HNS24866.1"
                     /translation="MLLLISPINTQEAQEAIDGGADIIDVKNPKEGSLGANFPWVIRS
                     IREITPKDLMVSATLGDVPYKPGTVALAAAGAVLSGADYIKVGLYGTRNYDEAR"
BASE COUNT         1013 a         1098 c         1106 g         1100 t
ORIGIN      
        1 ttcccccatg aaggaaccgg taagatcatc atccagctgc gccaggaggg ggatcttacc
       61 ggttccttca tgggggaagg cgtgtttaac actgttggta accagttcgt tgatgatgag
      121 ggcgcaggga atggcggtgt tgatgttgat ccagacgggt tcacagtcca tctccaggat
      181 gatcctctcc tggctgccct cgtaggagtt cagcacgttc tgcacaaggt ccttaatgta
      241 ctcggccaga tttatccggg caaaatcatg ggactggtac agcttttcat gcaccagggc
      301 catggattgg atgcggttaa tggtcttacg gaagaggtgg atggtttctt cgtcatcaag
      361 gcggttggac tggaggctga tgaggctgga gatgatctgg aggttgttct ttacccggtg
      421 gtgcacttct ctcaggagga tctccttctc ctccagggag ctcttgattt tctcctggta
      481 ctccacttct ggggtgatgt cttccagggt ctccactgct ccaatcacct ccccctccga
      541 gtctaacagg ggaaaggcag tcatgcgaag ccacttgccg tcccggatct gggggaagaa
      601 tccagtggca ctgtagacca tctctgccac ttccatggat acaggtgggg tggagtacca
      661 cttgcagatc ccttcccagt cattctccag taaaagatcc gccatgcatg gcctctcttc
      721 cgggtaaaat acctgccagt ggtgggtggt gcccaagaca tccctcctct taatgcccgt
      781 gaagttttcc agggcctggt tccagaaaac cacctcatga tccgtgtcta tggcaaaggt
      841 gggtatgggt gagccgttga ttatgctctc caatcttttt tcattgttca agagactttt
      901 gtacagcttc tccctttcca tgatctcctg ggacagtttc acgttggcct cctccagttc
      961 ccgggttctc tcctctacca ccttctccag gttctcccgg tgccgggcca gttggagctc
     1021 caccttttta cgatggtcca cgtcacgtat aaccagtaat aaaccatttt cttcctgatt
     1081 ttcatccaga aatgggttga tcaaggattc cagccagtgg taaccaccct ccgcattttt
     1141 gcatcggaac tcttcctgga cactggtgga ggatgattta accccttcca gggcacgggt
     1201 cagcttaccc tgatcatcgg ggtgcaccaa ctctaggaac ctgttgaggg gctggcctag
     1261 aaagtcctgg tgatggtatc ccaggatggt gtgcagggag ctgctcaggt actgtatctg
     1321 gctgtggaca tccacctgca tcaccatgtc ctgtatgttc ctggttatga ggcgcagctg
     1381 agcttccctg ttttccaggg accgggcggc tctatccaat tcacgggctt cttccacctc
     1441 atccaaagcc cttcttatgg tgtatggtaa cttggagatg ttgttcttga aaacatagtc
     1501 cttagcacct tccttcagga cttctaccac gaattctccc ccaattttgc cactgaagaa
     1561 gatgaagggg acctggggga tctgctgctt tgcgatcttc agggcggatt taccatcaaa
     1621 ggagggcagg gaatggtcag ccaggatgat gtggggctgg aattccctga ttcccctaag
     1681 aaattcctcc tcgttctcca ccctgcaggt ggtgaagggg atccctcctc tctccagttc
     1741 cttttctgcc agttcagcat cgaagggaac atcttccagc atcaatatcc ttattctatc
     1801 catcaaacca cactataccg gatactcaga attttaatgt tactatatct gctactattt
     1861 attaaccttt gtaaaacctt cccaagaacc cctatccata ttaatcatca tcgtcactgg
     1921 ttggattgcc ttacaaaccc gcctggatag ggtgggacga agatccgtgt cccctatgaa
     1981 tcccgtgcag cgacttgttc ccccgggggg atccatggta ataacattat aatagcaatg
     2041 ctgattataa ttgctgttat ggaggataga tacagaaaca ccaggacagc cctggacagt
     2101 atgaccagca ctgtaccacg ggtacagccc caggataagg tggctagtat caagcaactc
     2161 ttatccaccc aggcctgtca actgggtaac atcgactaca tctacgttgt aaatagacag
     2221 ggacatctag atggggtgct gtccatcaag gagattttct gttctttgga ctcggaggtg
     2281 actgccaggg agataatgaa gcaggacctg gtggtggcct ctccctccac ccaccaggaa
     2341 cgggtggtgt acctggccct gtcccatggt attaaggccg tgccagtggt ggatgaggac
     2401 ggcctattcc agggggtggt gccctacgac cagatcctgc gcatcttcaa ccacgaggtc
     2461 cagagtgacg tgtttaaatt tggcggaatc ttccacaagg tagggaagga gtacaccagc
     2521 atccagtcca gcaccaggat gatgatccgc tcccgcctgc cctggctggt ggtgggggtg
     2581 ctgggtggga ccatggccgc gggcctaatt ggctactttg agaacctgct ctcagagtac
     2641 ctggtactgg ccttcttcat cccggtgatg gtgtacatga gcgatgcagc cggaacccaa
     2701 tccgaggccc ttatcatccg cagcctagcc ctggacccta aactggccat gaaatcctac
     2761 ttcatccggg agctcaaggt ggcgggggtg atagggctgg tttccggtgt actggcagca
     2821 ttcacctccc ttctgggatg gacccagcca gttctcagtc ttattgtggg tttttccatg
     2881 tttatcagca tcatttcctc ggtagccatt gcaacggtgg ctcccctgat tttctacaaa
     2941 ctgaaatatg acccggcagt ggccaccgga cccctggcca ccatagtcag tgacatcacc
     3001 accctggcca tctacttcac aactgccatg gccctaactg gattaatcca ttaaaaaaaa
     3061 atcacgttaa caacttacac agttgtgaaa gtaaaacaac caataaagtt gtgtgggttt
     3121 cacaacccat aatgttgtat atcattattt acttattact agttgatgaa acaaaaactc
     3181 tacctcctgt ggggtgaaac tgcaccctgg gggttggtgg atgattacca gtcccggttg
     3241 gaggttattt gccgggtggt gaactccaga tacggtaaac acctgcacac ccccctctca
     3301 ccactccagg atccctggaa gctgggggcg gtgctgttta atatctcccg ggtttacgat
     3361 attctggaca ttgtactgaa gggcctgtat ccccagtcca tgcacttggt actggtgtac
     3421 gactacctgg atgaactcaa ggaaaaggac atcaaccacc tggatggacc tgccctggaa
     3481 aaggctaccc agatgatgtc caagcttgaa agaaacggtc tactctttga gatgaaggta
     3541 gataagaaag aaactgatct agcactatct ggtctgataa actctgtgca acttcttaag
     3601 aaggggttgt cagctaaaaa aaggcgtata ataagtgaat atgttaagac gggtaagcag
     3661 agcttggcag cggagaacct gaacatgacc cagcaggggg tgagcaaggc cctgaagagc
     3721 agtaactggc gggaaataaa ggacggggaa caaaaactcc aaagggccct ggaaatatac
     3781 agtgaccgtc tgggtcgaaa gcagcgtagc ctggatgatt tttattaaaa cccgggcctg
     3841 gtataaatct gctcccgggc tgatatcttc ctaggaaaag ggcaggaggt ggatccttcc
     3901 tggtggggag tttgtggatg ggggaggaac atctttatat ggcagtattc caatattagg
     3961 gtaagattat tcagctaagt atctcgtaaa ttttagtcct gttacaattg gaggaattaa
     4021 ctttgcttct cttaatcagt cccattaata cccaagaggc tcaagaagcc attgatggtg
     4081 gagccgatat aattgatgtt aaaaacccca aggaggggtc cctgggagcc aacttcccct
     4141 gggttatacg cagtatacgg gagatcaccc ctaaggactt aatggtaagc gccaccctgg
     4201 gggatgtgcc ctacaaaccc ggcaccgtgg cactggccgc tgctggagca gtactatccg
     4261 gagctgacta cataaaggtg ggcctctacg gcacccgtaa ctatgacgag gcccgtg
//
LOCUS       DAOQVW010000063         4290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_22975_length_4290_cov_3.505077, whole genome shotgun sequence.
ACCESSION   DAOQVW010000063 DAOQVW010000000
VERSION     DAOQVW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22975_length_4290_cov_3.505077"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKK85_02215"
     CDS             <1..283
                     /locus_tag="PKK85_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNS24867.1"
                     /translation="ELESIKILIEEGINTYCKVVVLPSTSEDEIGWIASRVKQDVQKS
                     SKLLMVLQPASPLENWAHCQSKLLGISAETGKHLDVLTIPQVHKLLGLR"
     gene            295..855
                     /locus_tag="PKK85_02220"
     CDS             295..855
                     /locus_tag="PKK85_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS24868.1"
                     /translation="MEMDTKVTVHDAMTSRVITVEPQTSVAEAARIMTERNIGSLIIK
                     SNAEPEGLITESDIIAKVVSKDLKASQIQVGDLMTQNLIRVHPGSELNEAARVMAKNN
                     IRRLPVVNNGVLVGILTTTDVVSVSPELTEILVENARMETSLDYSNNPESVPGACEVC
                     GNYVEFLDEFDGKFVCEECKEDLEGE"
     gene            857..1672
                     /locus_tag="PKK85_02225"
     CDS             857..1672
                     /locus_tag="PKK85_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS24869.1"
                     /translation="MPTVGNVMTHDPVTVSSEAHATHVRSLFRNEGFRSIPVISNNRL
                     EGVITRGDMLSISSTKSNIDARGIMEHPQVIATPHMGIKDLARNLLVAGQVYAPVVES
                     IENMKLVGIVTVADLLGYFVSKGIKPVKETIMDVYTPEVVTCDQHELISRVWNRMDDN
                     GFSGLPVLKKNKLVGIITRKDIIDSGNIRINRESGDMRRSATVEKIMKTPPLVVTPLT
                     PTSKTAQIMLEFNVGRLPVVENPIYIKKEPQRAKESDLIGIVSREDILWSYTG"
     gene            1765..2706
                     /locus_tag="PKK85_02230"
     CDS             1765..2706
                     /locus_tag="PKK85_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS24870.1"
                     /translation="MRKKQTINLVKSMDRGPVEFKTRASEHDGDVMSIAKKNVVTAPQ
                     TATIKEAAEIMVKNKFRRLPITDPGTDKLLGIVTSMDILDFLGGGDKYKILEEKYQDN
                     FPAAVNESVKEIMTREVEVLTTKSSITQAVEEMINKGIGALPIVDSEYKVNGIVSERD
                     FVMLLAGVLTDELVEDFMTKKVITTTPGTRIEGASKIMVRNRLRRIPVVGEDRKTPHP
                     ENEKLVGIVTATDVLEFLGKNQAFDRMITNSAEDVLNTTITEIMEDQVISVEPMTRLG
                     NTCELMNDKGIGGLPVIKNGIIQGIITERDILNAIKG"
     gene            2744..3583
                     /locus_tag="PKK85_02235"
     CDS             2744..3583
                     /locus_tag="PKK85_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS24871.1"
                     /translation="MKIKDVMNEEVVVMQDNEQVSHARNLMLKHGFSRIVVLDNQEIP
                     VGVVTEKDLTRKMGGKSPAWRRRPIDKISIRRVMTSNPITTTPNKGIRDAVELMIKNN
                     ISSIPVVDEDGLAGIITKTDIMQFYSEKLGGRWKVSDLMTGDVITVNENHSIGHVISV
                     MEEKNIGKVIVIRDSLPIGIITPANISFANVEDPETGVSVEKIYFLRKTEGLDKKNVR
                     EVSMLTAGDIMSNHLVTIQMDEDASRAAQMMMENKISGLPVVNNEELVGIITKTDLIR
                     GIQ"
     gene            3607..>4290
                     /locus_tag="PKK85_02240"
     CDS             3607..>4290
                     /locus_tag="PKK85_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS24872.1"
                     /translation="MHVKNIMSKKVVVIDKDQNIHDALKSMKKHKVSRLPVVNTNHKH
                     QKELVGIITEKDIAAHLGSSKYGNLPPSHFHISTVMTSSPVTVGSDKNLGAAAQMMLE
                     NRIGGLPVVNNGELVGMITKSDFMDTCQGRPFNEIKVEKYMNKEIITVSPQDRLVHAR
                     RVIIDDDLGRVIVTEDEDIQGILTAKDIAMAMISFRKMVPDKYKTSRIRNLLVEDVMT
                     QNVKTINKDA"
BASE COUNT         1452 a          832 c         1006 g         1000 t
ORIGIN      
        1 ggagcttgaa tccataaaga tattaataga agaggggata aatacttact gtaaagtggt
       61 ggtactgccc agcacatcag aggacgaaat tggttggata gcctccaggg ttaagcagga
      121 tgttcaaaaa tcctcaaaac ttctcatggt gcttcaacca gcgagtccac tggaaaattg
      181 ggcgcattgc cagagcaaat tattagggat ctctgcagaa acaggtaaac atctggatgt
      241 attgaccata ccccaggtac acaaacttct gggcctgaga taggaggttt tgaaatggaa
      301 atggatacca aggtcacagt acacgacgca atgacatcaa gagttataac cgtagaaccc
      361 cagaccagcg ttgccgaagc agcaagaata atgaccgaga ggaatattgg aagcctcatc
      421 attaaaagca acgcagaacc tgagggactg attacagaaa gtgacataat cgccaaggta
      481 gtatccaagg acttaaaggc cagccaaata caagtcggcg atcttatgac tcaaaacctt
      541 ataagagttc acccgggaag tgaattgaac gaagctgcca gagtaatggc taaaaataat
      601 atcagaagac ttcccgtagt taataacggt gttttagttg gaattctcac cactactgat
      661 gtggtcagtg tctccccaga actcacggag atactggtgg agaacgccag gatggagact
      721 tcattggatt attccaacaa tccagaatca gtgcccggtg cttgtgaagt atgcggtaac
      781 tacgtcgagt tcttggatga atttgatggt aagtttgtct gtgaagaatg caaggaagat
      841 cttgaggggg aataatttgc ccactgttgg taatgtaatg actcatgatc cagtaactgt
      901 atcttcagaa gcccatgcta ctcacgttag atcacttttt aggaatgaag gcttcagatc
      961 cataccagtt atatctaata atcgattaga aggagttata acccgcgggg atatgttaag
     1021 tatttcatct accaaatcca acatcgatgc tcgtggaatt atggaacatc ctcaggttat
     1081 tgccactcca catatgggga tcaaagattt agcccggaac ttactggtcg ccggccaagt
     1141 ctatgcacca gtggtagaat ccatcgagaa catgaaattg gtgggtatag tcacggtggc
     1201 tgatcttcta ggatactttg tttccaaggg aataaaaccg gttaaagaaa caattatgga
     1261 tgtgtatact ccggaagtgg ttacttgtga tcagcatgaa ctcatatcac gagtatggaa
     1321 ccgaatggat gataatggct tttctggcct tcctgtattg aaaaaaaata aattagtggg
     1381 aattataacc aggaaagata ttattgactc aggtaacatc cgaatcaatc gggaatctgg
     1441 ggatatgaga cgttcagcca ctgtggaaaa gatcatgaaa accccacccc tggtggtcac
     1501 ccccctaact cccacaagca aaacagcaca aataatgttg gaatttaatg tggggcgctt
     1561 acccgttgta gaaaatccaa tatatatcaa aaaagaaccc caaagagcta aagaatcaga
     1621 tttgattggt atagtttcaa gggaagatat tttatggtca tatacagggt gaatatccag
     1681 atttcatctt taaatttggg tataaaaact tgttctaaga atttatagtg ttattaagat
     1741 tagtatctca taagaggtgt atagatgagg aagaaacaaa ccataaacct ggtaaaatcc
     1801 atggaccgcg gtcctgtgga atttaaaacc cgtgcctcag agcatgatgg agacgtgatg
     1861 agcatagcca aaaagaatgt ggttactgca cctcaaacag ccaccatcaa ggaagcagcg
     1921 gaaataatgg taaaaaacaa gttcagaagg cttcctataa ccgatcctgg cacagacaag
     1981 ttgctgggaa tagtgacctc catggatatc ttggactttc tgggaggagg agataagtac
     2041 aagatcctgg aagaaaaata ccaggataat ttccctgctg cagttaacga gtcagtgaag
     2101 gaaataatga cccgggaggt ggaggtactc accaccaaaa gttccattac ccaagctgtg
     2161 gaagaaatga tcaataaggg aattggagct ttacccatag tcgattcaga atataaagtc
     2221 aatggaattg tttctgagag agactttgtg atgctcctgg ctggagtttt aaccgatgaa
     2281 ttggtggagg acttcatgac caaaaaggtc attaccacca cccctggaac cagaatagaa
     2341 ggtgcctcta agataatggt acgcaaccga ctccgcagga taccagtggt gggagaagat
     2401 agaaaaacac cccatcccga gaatgagaag ctagtgggca tagttacagc caccgatgta
     2461 ctggagttcc tgggtaaaaa ccaggccttt gatagaatga tcaccaacag tgcggaggat
     2521 gttttaaaca ccaccatcac cgaaattatg gaagaccaag ttatttcagt ggagcccatg
     2581 actcgactag gaaacacctg tgaattaatg aatgacaaag gaattggcgg tcttcccgta
     2641 attaaaaatg gcataattca gggaataatc actgaaagag acatactaaa cgcaattaaa
     2701 ggctaaccaa aaatagtccc atcctagggg atgatataat atgataaaaa ttaaggatgt
     2761 tatgaacgag gaagtcgttg taatgcaaga taacgagcaa gtcagccatg cccgtaatct
     2821 aatgctaaaa catggtttca gccgtattgt ggttctggac aaccaggaaa ttccagtggg
     2881 tgtggttaca gaaaaagatc ttactcgcaa aatgggtggt aaaagtccgg cctggaggcg
     2941 aagacccata gacaagatat ccatccgcag ggtaatgact tccaatccaa ttacaaccac
     3001 gcccaataag ggaataaggg atgcagtgga gttgatgata aaaaataaca tcagttccat
     3061 tcctgtggtg gatgaagatg gcctggccgg tataatcact aaaactgata tcatgcagtt
     3121 ttacagcgaa aaattagggg gccgatggaa agtatctgat ctaatgactg gtgatgtgat
     3181 aactgtgaat gaaaaccaca gtattggtca cgtcataagt gttatggaag aaaaaaacat
     3241 cggtaaagtt atcgtaataa gggacagcct gcccattggc atcatcaccc cggccaacat
     3301 ttcctttgca aacgtggaag atccggaaac aggagtcagt gtggaaaaaa tatacttcct
     3361 tagaaaaacc gagggcctgg acaagaaaaa tgtccgagaa gtatcaatgc taactgcagg
     3421 tgatatcatg agcaaccacc tggtaacgat tcagatggat gaagatgcat cccgggcagc
     3481 acaaatgatg atggaaaata aaatcagcgg attgccggtg gtaaataatg aagaactggt
     3541 gggaatcatc actaaaaccg atctgatccg cggaattcag taataatcaa acgaggggat
     3601 gcaaaaatgc atgtaaagaa tataatgtcc aaaaaagtgg tagtgattga taaagaccag
     3661 aacattcacg acgccctcaa gtcaatgaaa aaacacaaag tatctcgcct accagtggtg
     3721 aacaccaacc ataaacatca aaaagaattg gtgggtataa tcacggaaaa ggatattgcc
     3781 gcgcatctgg gatcatccaa atacggaaat ttacccccat cccattttca catttctacg
     3841 gtgatgactt cttctccagt aacggtgggc agtgataaaa acctgggagc agcagcacag
     3901 atgatgctgg aaaataggat aggcgggctt ccagttgtta acaacggaga actggtgggc
     3961 atgatcacca aatcagactt catggatacc tgtcagggtc ggccattcaa cgaaatcaag
     4021 gttgaaaagt acatgaataa ggaaatcata acagtatcgc cccaggatag gttggtccac
     4081 gcccgcaggg tcatcatcga tgatgatctg gggcgggtta tagtaactga ggatgaagat
     4141 atccagggaa tactcactgc caaggacatt gccatggcca tgatatcctt ccgaaaaatg
     4201 gtaccagaca agtacaagac atctcgaatc agaaacctcc tggtggagga tgttatgacc
     4261 cagaacgtta aaaccatcaa caaagatgcc
//
LOCUS       DAOQVW010000064         4282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_23032_length_4282_cov_3.708540, whole genome shotgun sequence.
ACCESSION   DAOQVW010000064 DAOQVW010000000
VERSION     DAOQVW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23032_length_4282_cov_3.708540"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKK85_02245"
     CDS             complement(<1..358)
                     /locus_tag="PKK85_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNS24873.1"
                     /translation="MSIYELAPEEVFLEMNTSTNGLTDDEAQKRLESYGANQIEEVKQ
                     KPIIYKFLANLYQLLALLLWAASFLAFLSGTPQLGAAIIAVILINAVFSFWQEYQAEK
                     AIEALKKILPSRAKVLR"
     gene            complement(444..1631)
                     /locus_tag="PKK85_02250"
     CDS             complement(444..1631)
                     /locus_tag="PKK85_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNS24874.1"
                     /translation="MLQIAVTGKPNVGKSSFFNAATLSEAEVAAYPFTTIDAHKAVAH
                     VTAACPCRELEIECSPRNSKCVDGVRLIPVELVDVAGLVPGAHEGRGLGNKFLDDLRQ
                     ARAFLHIIDASGSTDEEGRPCEPGSHDPLEDVDFLQYEITMWLYGILKKNWNRLLRKS
                     LSERLDFAKVMAEQLSGTGILLEHVLEAKRDIDKDYHQWDQEDTIQFLDVLLRISKPM
                     LIVANKADIHPAEENIRRLEKKYGEVVPASAAAELALIRAAEAGIIAYQSGDSDFEIL
                     KKDELSPEQEKGLLYIREHVLERYGSTGVQEALNRTVYDILGMMVAYPVEDEHNMSDQ
                     KGNILPDALLIPQGSKPRDMAFLIHTEIGEGFMHAMDARSCRRVASDYKIKHGDIISI
                     ICR"
     gene            1741..2364
                     /gene="cbiE"
                     /locus_tag="PKK85_02255"
     CDS             1741..2364
                     /gene="cbiE"
                     /locus_tag="PKK85_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HNS24875.1"
                     /translation="MSKIYLVGIGPGSTDYITPLAFKTVKLADVLVGSRRALQLFPDY
                     QGPTLELRAKNMDEMMDQTVKLARGGNTVVVLSTGDPGFSGVLKPIQNLARDVEVVVV
                     PGISSLQLCAARLLLPWDEANLLTLHGKGNSAPIWDVIDNGRPTLVLPDFRVHELAQY
                     LLDNGADPWRQVAVGERLSYPDEKIFHGTLEEAAHTEFSYLCVMVIY"
     gene            complement(2399..3376)
                     /locus_tag="PKK85_02260"
     CDS             complement(2399..3376)
                     /locus_tag="PKK85_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HNS24876.1"
                     /translation="MAFKDLFRFNKGKTTFVFIGGKGGVGKTTVSAATALWLADEGKK
                     TLIVSTDPAHSLSDSFERNIGHDPTPIAENLFAAEIDPEVSMKDYQAKMKEQQALNPG
                     MDFGMLEDQMDMASMAPGIDETAAFDKFLQYMNTDEYDIVIFDTAPTGHTLRLLSFPE
                     MMDSWVGKMIKIRRQVGSMAKAFKNIMPFMGDEEEEDRALEDMEESKKQIRAARGVLS
                     DPNRTSFKMVVIPEEMSIYESERAMEALKKYNMFADGVIVNQIQPPEADCEFCRARRK
                     IQEKRLETIAQKFGDQVIAQIPLFEEEVKGIDRLRGVAQILYQEPVADN"
     gene            3664..>4282
                     /gene="nadE"
                     /locus_tag="PKK85_02265"
     CDS             3664..>4282
                     /gene="nadE"
                     /locus_tag="PKK85_02265"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNS24877.1"
                     /translation="MTDDKQIMRKINVDKALRDISNFIEYSLIESNTQGLVMGISGGL
                     DSSTVATISAGVVNPEKILALAMPTSSTPPGDTEGAVELAKDLGIAYKVLDLDPLLTP
                     VENICRGGLDEEHIKLAQANLKARMRMMVLYYHANALNRLVVGTSNRTELMVGYFTKY
                     GDGGADILPLGDLYKTDVCSLAEHLDLPTSILEKKPTAGLWPGQTD"
BASE COUNT         1108 a          994 c          991 g         1189 t
ORIGIN      
        1 gacgcaacac cttagcccgg gagggcagaa tcttcttcag ggcttcaatg gccttttcgg
       61 cctgatattc ctgccagaaa ctgaaaacag cgttaattaa gataactgca ataatggccg
      121 ctcccaactg tggggttcca cttaaaaatg ctagaaaact ggctgcccat agtaaaagtg
      181 ccagtaactg atatagattg gctaaaaatt tgtaaattat aggtttttgt tttacttcct
      241 cgatttggtt ggccccgtag ctttccaatc ttttttgagc ttcatcatca gtcagaccat
      301 tcgtggatgt attcatttcc agaaaaactt cttcaggggc aagttcgtat attgacatag
      361 ttacacttga aacctacctc ccagtggcag aaaattcttt ctaatcagga caatctccca
      421 ggatcaagtt aggctccggg tcattaacga cagataatgc ttataatatc tccgtgtttg
      481 attttataat ctgaggccac cctacgacag ctacgggcat ccatggcgtg cataaaaccc
      541 tcacctatct cggtgtggat caagaaggcc atatcacgtg gtttggagcc ctggggtatt
      601 aaaagtgcat cgggtagaat gtttcccttc tggtcactca tgttatgctc atcctccact
      661 gggtaggcta ccatcatacc tagaatgtca taaaccgttc ggttaagcgc ttcttgcact
      721 ccagtggatc cgtacctttc cagaacatgt tccctgatgt aaagaagtcc tttttcttgt
      781 tcagggctta attcgtcctt tttcaagatc tcaaagtcag aatctccaga ctggtaagct
      841 ataattccgg cctctgcagc acggatcagg gccagttccg ctgcagccga ggcaggaacc
      901 acttcgccat attttttctc caggcgacga atattctcct cggcggggtg tatatcagcc
      961 ttgttagcca cgattagcat gggtttggag atacggagaa gtacatccag aaattggatg
     1021 gtatcctcct ggtcccactg gtgataatct ttgtcaatat ctcgtttggc ctccagaacg
     1081 tgttccagga ggataccggt gccgcttagc tgctcagcca ttaccttggc aaaatccagc
     1141 ctttcagata atgattttct taaaagtcgg ttccagtttt tcttcagaat accatatagc
     1201 cacatggtga tctcgtactg gagaaaatcc acgtcttcaa gggggtcgtg gcttccaggt
     1261 tcacagggac ggccttcctc atcagtggag cccgatgcat caatgatgtg caaaaaagcc
     1321 cgggcttggc gcagatcatc caggaatttg tttcccaatc ctcgaccttc atgtgctccg
     1381 ggtactaatc cggccacatc aaccagttcc actggtatta agcgcacacc atcgacacat
     1441 tttgagttgc gggggctgca ttcaatttct aactccctac agggacaggc agctgttaca
     1501 tgggccacgg ctttatgggc gtcaatggtg gtgaaaggat aagctgctac ctccgcttcc
     1561 gagagggtgg ctgcattgaa gaatgatgat ttgcccacat tgggctttcc ggtaactgca
     1621 atctgtagca tgatatcact gattatttat aattttttga ttattaaatc ttatttaata
     1681 ggtataaacc atataaaata acttagaaaa atggtgttca tataataaag gagttattac
     1741 atgtcaaaaa tttatttagt gggaattggg cctggatcaa cggattatat tactccactg
     1801 gcctttaaaa ccgtgaaact ggcagatgta ttggtgggta gcaggagggc tttgcaacta
     1861 ttccctgact accaaggccc caccctggaa ctccgggcta aaaacatgga tgaaatgatg
     1921 gatcaaactg taaaactggc aagaggagga aacacggtgg tagtattgtc aactggcgat
     1981 cctggattct caggagtact aaaacccatc caaaacctgg cacgggatgt tgaagtggtg
     2041 gtggtgcctg gtatcagttc cctacagcta tgcgctgccc gattactgct cccatgggat
     2101 gaagccaacc tcctcaccct acatgggaag gggaattccg cccccatctg ggatgtaatc
     2161 gataatggaa ggcccaccct ggtcctgcct gacttccggg ttcatgaact ggcacagtac
     2221 ctgttggata atggtgcaga tccctggaga caggtggcgg ttggtgagag gttaagctac
     2281 cccgatgaga aaatattcca tggcaccctg gaggaggcgg cccacaccga attcagctat
     2341 ctgtgcgtga tggtgattta ttagtaaaaa aagattagta aaaaaataga aaagggccct
     2401 aattatcggc aactggttct tggtaaagta tctgagctac acctcgtagc cggtcaatac
     2461 ccttcacttc ttcctcaaat agtgggatct gggcgatcac ttggtcaccg aatttttgtg
     2521 caatagtttc cagacgtttt tcctggatct tacgacgggc ccggcagaac tcgcagtctg
     2581 cctctggggg ttggatctgg ttaacaatga ctccatccgc gaacatgttg tactttttaa
     2641 gcgcttccat tgcccgttct gattcgtaga tggacatttc ctcagggata accaccatct
     2701 taaatgaggt gcggttggga tctgataaaa ctcccctagc agcccggatt tgctttttac
     2761 tttcctccat atcctctaat gcccggtctt cttcttcctc atctcccatg aagggcataa
     2821 tgttcttgaa ggccttggcc atgctgccta cttggcggcg gattttgatc atctttccca
     2881 cccaactgtc catcatctct gggaaggata acaaacgcag ggtatggccg gtgggggctg
     2941 tgtcgaagat gacgatgtcg tactcgtctg tgttcatgta ctggagaaac ttgtcgaaag
     3001 ccgcggtttc atcgatacca ggggccatac tggccatatc catctggtcc tccagcatac
     3061 caaagtccat acctgggttc agggcttgtt gttccttcat cttggcttga taatctttca
     3121 tggatacttc agggtctatc tctgctgcaa aaagattttc agctataggg gtggggtcgt
     3181 gaccaatatt tctctcaaaa gagtcggaaa gagagtgagc agggtcagtt gagactataa
     3241 gggttttttt accttcatct gccaaccata gggcggttgc tgctgatacg gtggtcttac
     3301 ccactccacc cttaccacca atgaacacga atgtggtttt tcctttgttg aatcggaaca
     3361 agtctttaaa tgccattaat cttcctccat ttgatcttct ttaaaataat ctataggata
     3421 ataccaattt ttttgctaat ataacttcca taactctaga ctatcctatg tgaatcgttg
     3481 tggacatata aaaattgtgc aacatccata ttaggataat gacatataat aaaaccttgc
     3541 ctaggacccc ttacaccaac cccatcaaaa ttgatagggg ccttaaggga gttatatctg
     3601 ctttaaatgg atatttataa tataaaactt taaaataaaa atattttatt cattggtggt
     3661 cttatgactg acgataaaca gataatgcgg aaaattaatg ttgataaggc cctgagagat
     3721 atatccaact tcattgagta ttctctaata gaatccaata ctcaaggctt ggtcatgggt
     3781 atcagtgggg gtctggactc atccaccgtg gccacgatat cggctggtgt ggttaatcca
     3841 gagaaaatac tggccctagc catgcccacc agcagcaccc cacccgggga tacggagggt
     3901 gcagtggaac tggccaaaga cttgggaata gcttataagg tcttggatct ggatcctctc
     3961 ctgactccgg tggagaatat atgtaggggc ggcctggatg aggagcatat aaagctggcc
     4021 caggccaacc tcaaggctag gatgaggatg atggtcctct actatcatgc aaatgcactg
     4081 aaccggcttg tggtgggcac cagtaaccgc actgaactaa tggtgggata cttcaccaag
     4141 tatggggatg ggggggcgga catcctcccc ctgggtgatc tgtacaaaac agatgtgtgc
     4201 agcctagccg aacatctgga tctccccacg tccattctgg aaaaaaagcc caccgcaggc
     4261 ctatggcctg gtcagactga tg
//
LOCUS       DAOQVW010000065         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_23172_length_4262_cov_2.997861, whole genome shotgun sequence.
ACCESSION   DAOQVW010000065 DAOQVW010000000
VERSION     DAOQVW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23172_length_4262_cov_2.997861"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKK85_02270"
     CDS             <1..366
                     /locus_tag="PKK85_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS24878.1"
                     /translation="RHWRTASSTCLTFLTAKSTLEMTREVFESYTHGNHDASLWMSLT
                     KHNMFNAIKALKNRKEHLYYKVIRLAWRYNPKQLIWGHKWKLWVPLPSIGIHLESDFL
                     PPTRKCLEFMEAEASGMGD"
     gene            complement(409..1641)
                     /locus_tag="PKK85_02275"
     CDS             complement(409..1641)
                     /locus_tag="PKK85_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNS24879.1"
                     /translation="MNWADKNVLITGISGFVGSYLGRELIKRGANVHGFIRRRADGSL
                     PKNLKDRGVADKINLIEGDLIDITSLANAFDQSQPDYIFHLAAQSFVERSFHNAIETQ
                     YANCLGTANLLEAVRVKDLNTKIVFAGTSEEYGLVLSSEEQYQKAKREYGTIFPEPED
                     IPEVPIKETNPLRPMSPYAVSKVYGDFLMRNYYHSYGLNNVVSRGFNHEGAGRGIMFV
                     TSVIARQIMKLKFGEQDRILIGNLNSLRDWSHVEDIVQGYLLLADKGLKGEVYNQGSM
                     RSNSVLSYILLGLEEAGWSIKRIETLNGEKIIEEPTEMDQGPIFGIKFPKTRVDRMMM
                     EGELEYTMNDKGIDVYTDQGNVLIEFNPERFRPAEVPILLSDTKKIQKLGAKIKHSLS
                     DITKHQLNHFLKSDNRLQ"
     gene            complement(1703..2644)
                     /locus_tag="PKK85_02280"
     CDS             complement(1703..2644)
                     /locus_tag="PKK85_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS24880.1"
                     /translation="MRQKKVAVTGGLGFIGSHLVEELSRENQVLIIDDESSGHVENIE
                     HLSLDNISLDRGDINDADLVDLFQEVDYVFHHAALASVPLSVENPLLCNEVNVTGTLK
                     VLDAARQTGVKKVVLASTSAVYGDNHNLPLSEKEPVNPKSPYAVSKAAGELYCRIFSE
                     VYGLPTVALRYFNVFGPRQDPNSQYAAVIPKFIESILIGKQPVIYGDGEQTRDFIYVK
                     EVVEANIKSAESDLTGVFNIASGQRHTVNELFQIITQLMGEELEPVHADPRPGEIKHS
                     LADVELSKSLGFHPKTNFKDNLGETVRFFVEDHKLKA"
     gene            complement(2837..3265)
                     /locus_tag="PKK85_02285"
     CDS             complement(2837..3265)
                     /locus_tag="PKK85_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HNS24881.1"
                     /translation="MNKDHYFSLIILILIVTGALSVIYITINPQPSEKFTEFYVLNQE
                     GRAGNYPVNLAPGGEGKLFMVVKNNEFQPVNYRLMAQLDNITIYNEEFTLYSDEKREI
                     PLEFKVKNYGSQKLEMFLYKLPDTNNTYRYLFLVLNDSKE"
     gene            3464..3745
                     /locus_tag="PKK85_02290"
     CDS             3464..3745
                     /locus_tag="PKK85_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-converting hydrogenase A subunit A EhaA"
                     /protein_id="HNS24882.1"
                     /translation="MIVHVNVLSYLLALGLAVILGLALRLPLLPERPMRYSWSISVIF
                     PTAILSLGFYAMLVQLGYQGLMVAASTGVITALFCKFLLEKLVPRPEDS"
     gene            3745..4248
                     /locus_tag="PKK85_02295"
     CDS             3745..4248
                     /locus_tag="PKK85_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24883.1"
                     /translation="MNEVLGIIIAAIICWFNFVLIDTWMGLPENPGVKGAEVIGRAVK
                     KRGGSLAGGFFQGNIVCSPDASAGTLLASVGSYAIGIPEGGLIAATLVYFGNRLCADP
                     GYAGTTGALSITLIIYLTSMVGMKPEFYIVGMVLAIITIQGLAHPTASRLLGKLAQKM
                     GRHTKMT"
BASE COUNT         1094 a          951 c          886 g         1331 t
ORIGIN      
        1 cgacactgga gaaccgcctc ttccacttgc cttaccttcc tgactgctaa atctaccctg
       61 gagatgacca gagaagtttt cgaaagttac acccatggta accatgatgc cagcctatgg
      121 atgagtctca ctaaacataa catgttcaac gccattaaag ccctgaagaa taggaaagaa
      181 catctctact acaaagtcat acgcctagct tggaggtaca atcctaaaca gttaatttgg
      241 ggccataaat ggaaactctg ggtaccctta cccagcattg gcattcatct ggagagtgat
      301 tttttacctc ccaccaggaa atgcctggaa ttcatggaag ccgaggcttc tgggatgggg
      361 gattagtgtg gaaaaaataa ggtgctaatg gcttaaaggt agagttcctt attgtagtct
      421 gttatcactc ttcaagaaat ggttcagttg atgtttggtt atatcactta agctgtgttt
      481 tatcttggcc cccaatttct ggatcttctt agtatcagag agtagtatgg gaacctcggc
      541 gggtctgaat ctctcggggt taaattctat taaaacattt ccctggtcgg tgtagacatc
      601 tatcccctta tcgttcatgg tgtactccag ttccccttcc atcatcatcc ggtctaccct
      661 ggtttttggg aatttgatac caaagatggg gccctggtcc atctcggtgg gttcctcgat
      721 aatcttctca ccattcagtg tttcaattct ctttatgctc cagcctgctt cttccaatcc
      781 cagtaaaatg taactaagta cggagttgct tcgcatggaa ccctgattgt aaacttcccc
      841 cttcaagccc ttgtctgcca ggaggaggta accttgaaca atatcctcca cgtgtgacca
      901 gtcccggagc gagtttaggt tgcctatgag gatccggtcc tgctccccaa atttgagctt
      961 cataatctgc cgggcaataa ctgaggtaac aaacattata cctcttcccg ccccttcatg
     1021 attaaaaccc cgggagacca cattattcaa tccataggaa tgataataat tacgcatcag
     1081 gaaatctcca tagaccttgg agacagcata gggggacatg ggccttaaag gattggtttc
     1141 ctttattggt acctcgggta tgtcttcggg ttcagggaaa atggtaccat attctctttt
     1201 tgccttctgg tactgttcct ctgatgataa aaccaagcca tactcttcac tggttccagc
     1261 aaatacaatt ttagtgttta aatccttaac acgtacagct tccaggagat ttgctgttcc
     1321 caaacagttt gcgtactggg tttctattgc attgtggaag gagcgctcca caaatgactg
     1381 ggctgccagg tgaaaaatgt agtctggctg ggattggtca aatgcattgg caagagatgt
     1441 tatatcaatt aaatctcctt ctatgagatt tattttatcg gcaactcccc ggtcttttaa
     1501 atttttaggt aaggacccat cagcccgtct cctgatgaaa ccatgaacat tagcccccct
     1561 ttttattagt tctctcccta gataagagcc tacgaaacca cttataccgg tgataagcac
     1621 atttttatct gcccaattca tctgataccc ccatgctcta tttattgttg attaatatct
     1681 tatttaatgt agacctatgg atttaagctt ttagtttatg atcctccaca aagaaacgca
     1741 ccgtttcccc caagttatct ttaaagttgg tcttagggtg gaatcctagg gatttcgata
     1801 attccacgtc ggccagggaa tgttttatct cacctggacg aggatccgca tgtacaggtt
     1861 ctaattcttc acccatcagt tgggtgatta tttggaagag ttcatttact gtgtgccttt
     1921 gacccgaggc aatgttaaag acacctgtca gatccgattc agcgcttttt atgttggcct
     1981 ccaccacttc cttgacatat ataaagtccc tggtttgctc cccatcgcca tagatcactg
     2041 gttgtttacc tatcaagatg gattcaatga acttaggtat tactgctgca tactgtgagt
     2101 taggatcctg gcggggaccg aatacattga aatatcgcag ggctacagtt ggtaatccat
     2161 acacctcact gaatatcctg cagtataatt cgccagctgc tttactcact gcatatggag
     2221 atttaggatt cactggttct ttttcagata aaggtaaatt gtgattgtca ccataaaccg
     2281 ctgaagttga ggccaatacc acctttttta ccccggtttg gcgggctgcg tccagaactt
     2341 taagggtgcc agttacgttt acttcgttac atagtaaagg gttttctaca cttaagggta
     2401 cacttgccag ggcggcgtgg tggaaaacgt aatcaacctc ctggaaaagg tcaaccagat
     2461 ctgcatcatt gatgtcaccc ctatccaggc tgatattatc cagggagagg tgttctatgt
     2521 tctccacgtg tccagatgat tcatcatcga tgatcagtac ctgattttcc cgggaaagtt
     2581 cctccactag gtgggagcca ataaatccca atccaccagt tacagctact ttcttctgtc
     2641 tcattaacaa cccaccctgc agttgttctg cattagtcaa tttttttttg aaaccttctc
     2701 taaccttatt ataatattct tcaccaataa aatctatatt aaatacaccc tgatttgctg
     2761 atccatccct ggccagtatt acttgtaact ctcctattac ttggaataca cgtttttcag
     2821 ggtagaaggt actgctttat tctttagaat catttaagac caggaatagg taacgatagg
     2881 tgttgttggt gtcaggcagc ttgtacagaa acatctccag tttctggctt ccataatttt
     2941 taactttaaa ttccaggggt atctcccttt tttcgtcact atatagggtg aattcttcgt
     3001 tgtagatggt gatgttatcc agctgggcca ttaatcgata attcactggt tgaaattcgt
     3061 tgttcttgac caccatgaag agcttaccct ccccacctgg ggctaagtta acgggatagt
     3121 taccagccct cccctcttga tttaacacat agaattctgt gaatttttca ctgggttgag
     3181 gatttatagt aatgtatatg acagataagg ctcctgtaac tattaatatc agtataatta
     3241 gtgaaaagta atggtcttta ttcattgtta accctttttt tgtgctgtat aagttatgtc
     3301 tcccaacttc ctcttcatca aattataaat gttttttata aagaagaggg ttgttcccat
     3361 tatctccaag agattatagt attaaactat taatatttga taggaaaact gtttatgaga
     3421 gattaataac atttatatac agtagttatg ataaggtttt atcatgattg ttcatgtaaa
     3481 tgtcctaagt taccttctgg ccctgggcct agcagtgata cttggactgg ctttacggct
     3541 tcctttacta cctgaacgac ccatgaggta ttcctggagc attagtgtta tttttcccac
     3601 ggcaatccta tctcttggtt tttatgccat gctggttcaa ttgggatacc agggacttat
     3661 ggttgctgcc tccacaggag ttattactgc tttgttctgt aaatttctcc tggagaagtt
     3721 agtaccccga ccggaggatt cataatgaat gaggtactgg gaataataat cgccgccata
     3781 atctgctggt tcaacttcgt actcatagat acctggatgg gcctaccaga aaacccgggg
     3841 gttaagggtg ctgaagttat tggaagggct gttaagaaaa ggggcggaag cctggctgga
     3901 ggtttcttcc agggaaacat tgtatgctcc cctgatgcat cagccggaac cttactggca
     3961 tcggtaggta gttacgccat tggaattcct gaaggtgggc ttatcgccgc tactctggtt
     4021 tactttggta accggctctg tgcggatcca ggttatgccg gaaccaccgg tgccctgagc
     4081 attaccctaa ttatttactt gacatccatg gtgggaatga aaccagaatt ctatattgtg
     4141 ggcatggtac tggccatcat caccatacag ggattagcac atcccactgc ctcccgtctc
     4201 ctgggtaaac tggcccagaa aatggggcgc cacacgaaaa tgacttagaa ggtgagatca
     4261 tg
//
LOCUS       DAOQVW010000066         4252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_23260_length_4252_cov_2.956159, whole genome shotgun sequence.
ACCESSION   DAOQVW010000066 DAOQVW010000000
VERSION     DAOQVW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23260_length_4252_cov_2.956159"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1717
                     /locus_tag="PKK85_02300"
     CDS             425..1717
                     /locus_tag="PKK85_02300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS24884.1"
                     /translation="MKIVVIGGGPAGRSAAMEASKLEAEVTLIEKDKIGGKCLHQGCM
                     VVCGLNEVAKFYLDSQNYQKLDICNNITEINFPAVIRRLNETTENIKKVLEYETQSTG
                     VDVITGEAQIQKGSVLVSDDKVPYDKLIIATGSRAMIPPIKGVEHALTYADIPYLEDL
                     PEKLLIVGSGVIAAEFANIFSSFGTEVRIFCRNKFLKMLDPEIKDYVINNLLQKVQIT
                     EYTQVDEITDYGIETSRDTVEGQILLATGLIPNSEITQNMVKIGDHKEILVNPHMQTS
                     NPHIYAAGDVIGGVTNTPISRMEGVIAARNACGISSTMDYSWIPQSISLYYDVSFLSP
                     QDHQIGTEATIPGSAGPGSFWRVLEGKTGMTKVSVDLDEGSITGISSVSPSSRNNIAY
                     LAKMIRDGYKTPDFDDFLEVHPSNDVVYKLLRFFGEYS"
     gene            complement(1928..2977)
                     /locus_tag="PKK85_02305"
     CDS             complement(1928..2977)
                     /locus_tag="PKK85_02305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS24885.1"
                     /translation="MKALLFVTGRGIGGDAVTAYNISQALIRKGVECEFVLDHTAPGL
                     LFEKKGIKWHRSSIPQAGGHAATKSKLFKASIKTLNASLEAARLCRQIHPNVVVGVIG
                     GGAIVGCIAAIMARIPAVGILITPMDAKVCTKIATTIVLPESNLFTRKMNDKKIYKAY
                     SPVDPKIVEGNPARAMKKIPLGYDANKPTILISSGSTLFEDMARAASRLGEEGIDANL
                     MVVGEPLEQGYKKHFSYPNVFYLGYIDWIRDLYQLVDLAILTDDGMMIHEAMVFQLPV
                     VALLGVKYGRYHNLAEVFEGAVLESNLQDLPAVVKKAFKELEDMKKKTEHYGKDILAA
                     ADNIADAIIRTAKKE"
     assembly_gap    3252..3261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3433..4212)
                     /locus_tag="PKK85_02310"
     CDS             complement(3433..4212)
                     /locus_tag="PKK85_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall biosynthesis protein"
                     /protein_id="HNS24886.1"
                     /translation="MGLAPLTVLFLFFTPPYNFLVAIIGIFAFFDDLIGRRKINYLPI
                     ELGQLSRGLGMIIVMVMGYFYFGPVSILVAFMVQPLNIADMQPGVAVSTIIVMGSCMA
                     ILLYLVSGDPYPPLIMIAACVGYAPLDFQGKIMMGEVGNHSFAVSLGILFALSGNFLF
                     DSYAWGGWATFFFVFALLILTSILIAFLRQKNLQKFLKTQLKIPDPVFGDYVMDVLTG
                     GGLGDLLRRLIFKKKSVVVKNRLGILLGFRRLLYNPHSVSD"
BASE COUNT         1342 a          842 c          824 g         1234 t
ORIGIN      
        1 gctttaggaa catcagccac atcaggatca acaacgacac aatacttcgc cacttctcct
       61 ggatataaaa tttagggagc cggatgttca aatcaagtag cccctaccgg ttttattctt
      121 agttttggag gtattgactc atgaacttca ggatgtacaa agtacaaaat tctttgttca
      181 attaccgaat tggaaaagta ccctggtgat cgactgttta ggtttcctgc gtaagggtag
      241 aatatatggt agttcaatat catactatcg acacaagatc ctgataatgg agatgctttt
      301 actaacgtaa ttgtgcagag gtcctcaaat tataattgat tcatgttgaa ttcacagata
      361 ttcatacatt tattattaaa aatattaaat tagtgatgtg ctaagtcttt aggaggtctt
      421 tgaaatgaaa atagtggtta ttggaggagg tccagcgggc agatcagctg ccatggaggc
      481 atccaaactg gaagcagagg ttacgttaat agaaaaggat aagattggag gtaaatgtct
      541 tcatcagggc tgtatggtgg tttgtggcct taacgaagtt gccaaatttt acctagactc
      601 tcaaaactat cagaaacttg atatttgtaa taatataacc gaaatcaatt ttccagctgt
      661 aataaggagg ttaaatgaaa ccacggagaa tataaagaag gtgttggagt atgaaaccca
      721 gagcactggg gtggatgtaa taaccggaga ggcccagatc cagaagggta gtgttttagt
      781 cagtgatgac aaagttcctt acgataaact gatcatcgcc accggatccc gggccatgat
      841 accacccatc aagggggttg aacacgcctt aacctacgca gacattccgt atctggaaga
      901 tcttccagaa aaactcttaa tagtggggag tggagtgatt gctgcggaat ttgccaatat
      961 attttcttct tttggcacgg aggtcagaat attttgccga aacaaattct tgaagatgtt
     1021 ggatccagaa attaaagatt atgtaatcaa caatttactg cagaaagtgc aaatcaccga
     1081 atatactcaa gtggatgaaa ttacggacta cggaattgaa acatcccgtg acactgttga
     1141 aggtcaaatc ctgctggcca caggacttat tcctaactca gaaataaccc agaatatggt
     1201 gaaaataggt gatcataaag agatattagt aaacccccat atgcaaacca gtaaccccca
     1261 tatctacgct gcaggggacg ttattggagg agttaccaac acacccatat ccaggatgga
     1321 aggagttatt gcggcacgaa atgcctgtgg aatctcgtcg accatggatt acagttggat
     1381 cccccaatcc atatccctgt actatgatgt aagttttcta agtccccagg atcatcagat
     1441 agggacggaa gctaccatac ccggttctgc ggggccagga tcattttgga gggtgctgga
     1501 aggaaaaacc ggaatgacaa aggtctcagt agatttagat gaaggctcta ttacgggaat
     1561 atcatcagta tcgccttcat cacggaacaa catagcatat ttggctaaaa tgatacggga
     1621 tggatataaa acccctgatt ttgatgattt tttggaggtg catccctcca acgatgtagt
     1681 gtataaactg ttacgcttct ttggcgagta tagctaataa tatcatttaa tgattcacgt
     1741 aataaaaata ggaaatgaat cctataatat ccttccaaga aggaactgga cttcatagac
     1801 acgtgtatac tgattcaact ccatgactat ctagcaacaa aggtagctgt gatcgaaatc
     1861 gtctaaaaga tacccggata agtgtccatt gtgaagagta ccattttaaa aatagttgtt
     1921 ctttctatta ttcctttttg gcagtccgga taattgcatc cgcgatgtta tcggcagctg
     1981 ccaatatgtc ttttccatag tgctctgtct tctttttcat atcttccagt tctttaaaag
     2041 cttttttaac tactgccggt aaatcctgca aattactttc cagaactgcg ccttcaaata
     2101 cttctgccag attatggtag cggccatatt tcactcctaa aagtgccaca actggtagtt
     2161 ggaataccat ggcctcgtgg atcatcatgc cgtcatcggt aagaatggcc aaatccacca
     2221 actgatagag atcacgtatc cagtctatgt aacctagata gaaaacattg ggatagctga
     2281 agtgtttttt atatccctgt tccagaggtt ctcccaccac catcaagtta gcatcaatcc
     2341 cttcttctcc taaacgactt gcagcgcgag ccatatcctc aaaaagtgta gatccagaag
     2401 aaatgagaat agtgggttta ttagcatcgt accccaaagg gatctttttc attgcccggg
     2461 ccggatttcc ctccacaatt ttgggatcta ccggtgaata agctttgtaa attttcttat
     2521 cattcatttt tcgggtaaaa aggttagatt caggtaaaac aattgttgta gcaatttttg
     2581 tacacacttt agcatccatt ggtgttatca ggattcctac ggctggtatt cttgccatta
     2641 ttgccgcaat acatccaacg atggcccctc caccaataac tcccaccacc acgttgggat
     2701 ggatctggcg acaaagccta gcagcctcca aactggcatt cagggttttt atagatgctt
     2761 tgaataattt ggatttggtg gctgcatgcc cacctgcttg aggaatactg ctccgatgcc
     2821 atttgatacc ttttttttca aataataatc cgggtgcagt gtggtcgaga acaaactcac
     2881 attcaacacc ttttctgata agagcttggc tgatgttata agcggttact gcatcaccac
     2941 caattcctct tccagttaca aataagagag ccttcattaa atcacacatt cattaagtta
     3001 acaaccttta ttatattcat attgaacagg tttcataaaa attggatcca atatttatct
     3061 tatcaattaa tttagaaaat ccctatttta gattaaggac cacttatgaa cattctaata
     3121 tgtccgagta atcaaaatta ttaaaacctt tctagaactc attaataatg gttaaattgc
     3181 cattatattc caaacgttta tctaatagtt tgtaaggtta taactacatc aatagttttg
     3241 tttgaaacat tnnnnnnnnn ntaagtgaca atgatgaaag ttgggtttag actattgtag
     3301 gccgatatag ttttttttat cttcaccact ttttttagct ctatccacca tcctgaaaca
     3361 tcttaattaa gatggattta ttatttgaga ttgttacaaa atattttggt aagaaagatt
     3421 gtaattttct tgttaatcac ttacagagtg ggggttgtat agcaggcgac gaaatccaag
     3481 aagtattcct aatctatttt taacaactac actctttttt ttgaatatta aacgacgcaa
     3541 caagtcgccc aacccgccgc cagtcaatac atccatgaca taatcaccaa aaacagggtc
     3601 tggaattttc aattgggttt ttaaaaattt ttggagattt ttttgtctta aaaacgcgat
     3661 caaaattgat gtgagtatta aaagagcaaa tacaaagaaa aacgtagccc aacctcccca
     3721 tgcatatgag tcgaacaaaa aattaccgga caatgcaaac agtattccca agctaactgc
     3781 aaatgaatgg ttacctactt ctcccatcat gatttttccc tgaaaatcca agggagcata
     3841 tccaacacag gcagctatca taatgagggg aggataaggg tctcctgaaa ccagatatag
     3901 aagaatcgcc atgcaggatc ccataacaat aatggttgac accgcaactc caggttgcat
     3961 atctgcgatg ttcaagggct gtaccataaa cgccaccagg atggaaacag ggccaaaata
     4021 gaagtatccc atcaccatta ctatgatcat tcccaaacct cgggataact ggcctaattc
     4081 aatgggaagg tagttaattt ttctacgacc gataagatca tcaaaaaagg cgaaaatacc
     4141 aataatggca actaaaaagt tatacggtgg agtaaaaaat aaaaacaata ctgttaaggg
     4201 tgctaatccc actgccctag gggtaccacc cctaatagat gtgtaaaggt tt
//
LOCUS       DAOQVW010000067         4228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_23462_length_4228_cov_3.212078, whole genome shotgun sequence.
ACCESSION   DAOQVW010000067 DAOQVW010000000
VERSION     DAOQVW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23462_length_4228_cov_3.212078"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..636
                     /locus_tag="PKK85_02315"
     CDS             187..636
                     /locus_tag="PKK85_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNS24887.1"
                     /translation="MTDKNPLRGVYCLIINLKEKSTIQVGKKGKINFEDGCYVYVGSA
                     LNSLTARINRHLRAEKKLHWHVDYLLESDHSEIAEVMYTITHERLECQVAHEISKGGM
                     GVKGFGCSDCGCQSHLFYFSNCFGAVEACQDAFNHLKQEQKTLLDLI"
     gene            complement(652..1449)
                     /gene="cfbC"
                     /locus_tag="PKK85_02320"
     CDS             complement(652..1449)
                     /gene="cfbC"
                     /locus_tag="PKK85_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HNS24888.1"
                     /translation="MKKTRGIAIYGKGGIGKSTVVSNLAAAYSQDEKVMVIGCDPKAD
                     TTRTLVGRRIPTILDTMRTNKNAARGDVLFTGFSNVICVESGGPEPGVGCAGRGVIVA
                     MSLLEKLGIFEEDLGIIIYDVLGDVVCGGFAVPLREKYADEVYIVTSGEYMALYAANN
                     ISKGIKKLKGQLGGIICNCRGIEHEEDMVKEFAERIGSHVVGVIPRSELVQKSEINAQ
                     TVVEKFPESEQSQAYRQLAVSILRNRKFVVPEPMDVEEFEEFFHQFN"
     gene            complement(1451..1921)
                     /locus_tag="PKK85_02325"
     CDS             complement(1451..1921)
                     /locus_tag="PKK85_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS24889.1"
                     /translation="MKIEEAMQREVIKYQPEDRIVEVAQSLRDNKISGAPIVNKEGLL
                     VGIISEGDIMRLLEVHSPRINLILPAPLDLIELPVRMKYELDEIAEDMNKAASVLIGE
                     IMTTKVITIGPEDDLSDAAQLMDTHNINRLPVVDGSGKIVGLVTRGDIIAAMVR"
     gene            complement(2094..3323)
                     /locus_tag="PKK85_02330"
     CDS             complement(2094..3323)
                     /locus_tag="PKK85_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNS24890.1"
                     /translation="MIEIVELLAPARDFQALSAAINNGADAVYVGLIGHNMRANSGGF
                     SLEDLERAVRRCHNSRVKLYLCTNTMMRDKDLDDLRDKLPKINLLGVDALIASDLGVL
                     NLARQEGLDVHMSVQANVSNKESLRLLKELGVKRVILSRELSLSEIKEITEKNILEVE
                     VFVHGAMCLAISGRCFLSAHLYQKSANCGECRQPCRKEWKLTSQDAEELSIGKNFLEN
                     QKTGSNSSFGHLLSPKDLCMVEHIPDLMDAGISSFKIEGRARPADYVAAVTGVYREAM
                     DFYENGNWKFDEKWMEKLKKVFNRGFDTGFYYGVPHQISPSNQSTYRKEDIGEVVNYY
                     QKAQAAEIRLWSRLQEGDEVIIQGPTTGSVVQKVESLEMHGMGVGKAFKGDNVGLLVE
                     NRVRPRDVVYKLTKKTG"
     gene            complement(3430..>4228)
                     /locus_tag="PKK85_02335"
     CDS             complement(3430..>4228)
                     /locus_tag="PKK85_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS24891.1"
                     /translation="IETIPGTSQRVASIVDITDRKKSEKELKKLNEKLRKSNVELEQF
                     ASIAAHDLHEPLRMVVSFLKLLKQHNQDKLDPKAQKFIEYASEGAVRMQQMINELLTY
                     SRLSTRKKELRDVDCQKILDQVLMNLKLSIKDSKAKITIDPLPILTADYSQLLQLFQN
                     LIGNAIKYCGQDKPEIHISARKMGEEWLFTVEDKGIGIDPKYAQRIFMIFQRLHQAEY
                     PGTGMGLAICKKIVERHGGWMWVDSKLGEGSKFYFTIPVKSVPKIFI"
BASE COUNT         1121 a         1006 c          792 g         1309 t
ORIGIN      
        1 gctgggaggt aacgttggga tgtccatgtg ctttgaatgc gaattccata atttaaaact
       61 cctttattct tagaaatagt atccaaattc aacggcacag ataactaatt tgtttagtca
      121 tataataaaa tggttcaact caaaggtaac cattttacaa gataatgaca atactcaaag
      181 gataaaatga cggataaaaa ccccttacga ggcgtgtatt gccttattat taatttaaaa
      241 gaaaaatcta ccattcaagt tgggaaaaaa ggaaaaatta actttgaaga tggatgttac
      301 gtgtacgtag gctctgctct taattctcta acagcccgta ttaatcgtca cctaagggcg
      361 gagaagaagt tgcactggca tgtagactat ttacttgaaa gtgaccattc agaaatagca
      421 gaagtgatgt acaccattac tcatgagcgc ctggaatgtc aagtggcaca cgagatttcc
      481 aaagggggaa tgggggtaaa gggatttggc tgttcggact gtgggtgcca gtcccatctt
      541 ttctacttta gtaactgttt tggagcggtt gaagcctgcc aagatgcctt taatcatctg
      601 aaacaggagc aaaaaactct tcttgattta atttaggagc actttaataa actagttgaa
      661 ctggtggaag aactcttcga attcttccac atccatgggc tccggcacca cgaatttccg
      721 gtttctaagg atggaaaccg ccagctgacg ataggcctgg gactgttctg attctggaaa
      781 cttctccacc acagtttggg cattgatttc actcttttga accaactcgc tgcggggtat
      841 gaccccaacc acatgggatc cgattctttc agcaaattcc ttcaccatat cttcttcatg
      901 ctctattcca cggcagttac agattatgcc acccagttgt cctttaagtt tctttatacc
      961 cttagatata ttgttagcgg cgtagagagc catgtattcc cctgaagtta caatgtaaac
     1021 ctcatcagca tatttttcac gaagtgggac tgcgaatccc ccacaaacca catcccccag
     1081 tacatcgtag attattattc ctagatcctc ctcgaaaatt cctaatttct ccaggaggct
     1141 catggccaca attactcccc ggccagcaca acccacccca ggttcaggtc ctccactttc
     1201 cacacaaatt acattactaa agccagtgaa taaaacatct ccccgggcgg cgtttttatt
     1261 ggtacgcatg gtgtccaaga tggtggggat gcgcctccca accagggtgc gagtggtatc
     1321 cgccttagga tcacagccta tcaccattac cttttcatcc tgggagtagg cagcagccag
     1381 attagagacc acagtagatt ttccaatacc accctttccg taaatagcaa tacccctggt
     1441 ttttttcatc tcatctcacc atggcggcta tgatatcacc ccgggtcacc agacccacta
     1501 tcttaccact tccatccaca accggcagcc ggttgatatt gtgggtgtcc atgagttggg
     1561 cggcatcaga taaatcatct tcaggaccta tggttatcac cttggtggtc attatctctc
     1621 caataagcac cgatgcagcc ttgttcatgt cttctgcaat ttcgtccaat tcatatttca
     1681 tacgcactgg taactctata agatccaggg gagcgggaag gatcaggtta atgcgggggg
     1741 agtgtacctc taaaagtcgc atgatatcac cctcactaat gattcctaca agaaggcctt
     1801 ctttattcac gataggtgct ccactgatct tattatcccg caagctctgg gccacttcca
     1861 caattcgatc ctctggttgg tatttgatta cctctctctg catggcttct tctattttca
     1921 tcacatgacc atctccacta tcatttctaa aaagcatgtt tttatattaa cagtactatt
     1981 aacttccggg ggatcactac ccgttactat ggtttttcta ggattatttc ttttccatta
     2041 tgcgttgtat atttaaagaa aagataaaac tcatatatag ctagatgaac cctttatcct
     2101 gtttttttcg taagtttgta gacaacgtcc cgagggcgca cccggttttc aaccaataaa
     2161 cccacattat cccctttaaa agcttttcca acacccattc catgcatttc cagagattct
     2221 accttttgaa ctactgatcc agtggtaggt ccctggatta tcacttcatc cccctcttgg
     2281 agtctactcc ataatcgtat ctcagcagct tgtgcctttt ggtagtaatt aaccacctca
     2341 ccaatgtctt ctttacggta cgtggactga ttagaggggc taatctggtg gggcacccca
     2401 taatagaagc ctgtatcaaa tcctcggttg aaaacttttt ttagtttttc catccatttt
     2461 tcatcgaatt tccagttccc attttcataa aagtccatgg cctcccggta caccccggtg
     2521 accgcagcca cataatcggc gggccgggcc cttccttcaa ttttaaagga agatattccg
     2581 gcatccatga gatctggtat gtgttcaacc atgcaaaggt ctttcggact taacaagtgt
     2641 ccgaatgaac tgtttgaacc tgttttctgg ttttccagga aattttttcc aattgaaagt
     2701 tcttccgcat cctgagaggt gagtttccac tccttgcggc agggttgacg gcactcgcca
     2761 cagttagcac ttttctgata gagatgtgca cttaaaaaac accttccaga tatggctagg
     2821 cacatggccc cgtgcacaaa gacctccacc tccaggatat ttttttctgt aatctccttt
     2881 atttccgata gggataattc cctggagagt atcactcgtt ttacccctaa ttctttaaga
     2941 agccttaaag attccttatt ggacacatta gcttgtacac tcatgtgcac atccaggcct
     3001 tcttgtctag ccagatttaa aacacccaaa tcagatgcta ttaacgcatc tactcctaat
     3061 aaattaattt ttggtagttt atccctaaga tcatccagat ctttatcccg catcatggtg
     3121 ttggtgcaca ggtataactt caccctcgaa ttatgacagc gccttactgc cctttccaag
     3181 tcttccaggg agaaacctcc actgttggcc cgcatattgt gtccgatcaa gcctacatac
     3241 acggcatcag ccccattatt tatagctgca cttaaagcct ggaaatcccg ggctggtgcc
     3301 agtaattcaa ctatttctat caccttcaca tttagataac tctccgatta tcttcaggag
     3361 aatcttgttt aagttaatga tgtccttagg cattttttaa aattcattgt ttacatgatt
     3421 tatgaaaaat tagataaata ttttaggcac tgattttaca ggaatagtaa aataaaattt
     3481 agaaccttct cctaacttag aatccaccca catccatcca ccatgtcttt ccacaatttt
     3541 tttacagatg gccaggccca tccctgtgcc aggatactcg gcctggtgaa gtctctggaa
     3601 gatcatgaaa atgcgttggg catacttggg atctatccct atacccttgt cttccacggt
     3661 gaacagccat tcttcaccca ttttacgggc tgaaatatgg atttcaggtt tatcctggcc
     3721 acagtacttg atggcgttac ctatgagatt ctggaagagt tgtaataatt gagaataatc
     3781 agcagttagt atgggtagtg gatcaatagt gatcttcgcc ttactatctt ttattgacag
     3841 tttcagattc attaatacct gatccagtat tttttgacaa tccacatccc tcaattcctt
     3901 ttttctggtg ctgaggcggg aataggttaa tagttcatta atcatctgtt gcattctaac
     3961 cgccccctct gatgcatatt caatgaattt ctgagcctta ggatccaatt tatcctggtt
     4021 atgttgcttc aataatttta aaaaactaac aaccatgcgc agtggttcat ggagatcatg
     4081 agcagcaatg gatgcaaatt gttccagttc cacattggac tttctcaatt tttcattgag
     4141 tttcttaagc tctttttccg attttttgcg gtcggtgata tccacaatag atgctactct
     4201 ctgggaagtt ccgggtatag tctcgatg
//
LOCUS       DAOQVW010000068         4198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_23685_length_4198_cov_3.722182, whole genome shotgun sequence.
ACCESSION   DAOQVW010000068 DAOQVW010000000
VERSION     DAOQVW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23685_length_4198_cov_3.722182"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1867
                     /locus_tag="PKK85_02340"
     CDS             368..1867
                     /locus_tag="PKK85_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2193 domain-containing protein"
                     /protein_id="HNS24892.1"
                     /translation="MQELYEKMVKEALTAQKADVETIKKNRGTEFKIKDTKVYLDAVE
                     KMEAVGEQSESVINLHVDSVKTHFELLNSLTDTIRPEDDPFVEHYQTPAILEILGEED
                     PKFQASVDKFIEAVSKSEALVGREVVRRYGGFYGPTCVVDFALIPGSTSNLVNQILKT
                     VDAPLEHKQAILSAKSWGMNTSYGVGEVFAQELEAGATLSDAIDKEVAMIQEIYKNPV
                     EAQAQLMDSLGHESFDVRKYMAGYKDKMKKTIMAAVDDEVHYGNILTVPAYCVGDISH
                     HIAQSTYNMCKDDVVMGVIEATTAVMESTLQAALPNFQSVVEILSLATGSSACAVEYI
                     LELDGFNAPMVVDLLTKKFHNFVQLYPTRGAAAELHNCDFMDMIYRGWNHLDAARRDQ
                     NGSPGKLIPRIGNFAVDLEPVHQNEVIMNPQRYTYPACAITVRFSALMRLADYPCLLT
                     SEPVTATLMTNIIALNKKNPASPARTCKKCASACLVDFRHSHCEWKEAV"
     gene            1895..2104
                     /locus_tag="PKK85_02345"
     CDS             1895..2104
                     /locus_tag="PKK85_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HNS24893.1"
                     /translation="MKCYICAQEGKDSDALAICIVCGMGLCQEHIIHQEIELWEGGYP
                     FPSEKIKATLPRILCPYCYKALKGD"
     gene            2108..2860
                     /locus_tag="PKK85_02350"
     CDS             2108..2860
                     /locus_tag="PKK85_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HNS24894.1"
                     /translation="MASLAKRAIAELVGTFLLVFFGTGAAIITLMISAGQEPATTFNI
                     GIGVLGGLGDWLAIGLAFGFAIMASIYALGSISGCHINPAVTIGLWAVKKFPSQDVLP
                     YIIAQLIGASLASLTLVMALGMDAVTIGGLGATAPFPGVGYWQAIVVEAIGTFILVLT
                     IMGVAVDRRATPGFAGLAIGLTVAGVITTVGNITGASLNPARTFGPYLGDMLLGGSNL
                     WNYFPIYVVGPIVGAVVAALLYQYLISEDTTL"
     gene            complement(2921..3478)
                     /locus_tag="PKK85_02355"
     CDS             complement(2921..3478)
                     /locus_tag="PKK85_02355"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNS24895.1"
                     /translation="MKSKIIKTTPFGPVAILWSIVDDHPQINRVLISKPGYSTIEQVN
                     NINPKAKPSSCKEIDAVAAKISAYLQGEDVKFSLDMVNLDTCPPFQKRVLVADYLIPR
                     GCVSTYKFIAAHVGHKQAARAVGNALATNPFPIIIPCHRIIRSDKHLGGFQGGTIMKR
                     TLLENEGVVIDHTGRVVSPCIHYQK"
     gene            complement(3820..>4198)
                     /locus_tag="PKK85_02360"
     CDS             complement(3820..>4198)
                     /locus_tag="PKK85_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNS24896.1"
                     /translation="YLSGGFSSWYTQAHETEALKLQTVQELRKRQFDGDFYLLDVRKL
                     VDREKGFIEGSNHIYAGLVPQQLNDIPKNQEIVIYCDSGNKSTMVASYLLRKGFQNVG
                     SVLGSMEAWMEASYPVVVDHQGL"
BASE COUNT         1168 a          947 c          919 g         1164 t
ORIGIN      
        1 gatgttgtgg actcatcacc taatttttgg gaactttcta tgaacatttt taggggttag
       61 atagattgaa ctcccttttc tttgccatct tttgaataat taaatctcta ctgggtgaaa
      121 ttccttcatc aaaggaatca ttatccggaa atatttattt actacttcca tcataatatg
      181 aatgtacaca taaaaagata atggcagtcg ctggatttaa agaaaaccaa caggagaaaa
      241 aaaatccttt gatttttttt gactccttga actgctgtgg gggtagggat tttaccaatt
      301 tattttttcc atgccactac tcctgtgata ggatcacaaa aaatatgaag atatttgagg
      361 tgatagaatg caggaattat atgaaaaaat ggtgaaagag gccttaacgg ctcagaaagc
      421 agatgttgaa actattaaga aaaatagggg aactgaattt aagattaagg ataccaaggt
      481 ctacctggat gcagtggaaa agatggaggc cgtgggtgaa caaagtgaat cagttatcaa
      541 cctccacgtg gactcagtga aaacccactt cgaactttta aacagcctta ccgataccat
      601 ccgccctgaa gatgacccat ttgtagagca ttatcaaacc ccggccatcc tggagatcct
      661 gggcgaagaa gacccaaaat tccaggccag cgttgataaa ttcatcgaag cagtatcaaa
      721 atctgaagcc ctggtaggcc gggaagtggt ccgtcgctat ggtggattct acggccccac
      781 ctgtgtggtg gattttgccc tgataccggg cagtaccagt aacctagtaa atcagatact
      841 gaagaccgtt gatgcgccac tggaacataa acaagccatt ttatctgcca aatcctgggg
      901 aatgaacaca tcttatggtg tgggagaagt gtttgcccag gaattagaag caggggcaac
      961 cctgtctgat gccattgaca aggaagtggc catgatccag gagatataca agaatcctgt
     1021 ggaggcccaa gcccaactca tggattcctt aggacacgaa tctttcgatg tccggaaata
     1081 catggctgga tacaaagaca aaatgaaaaa aaccatcatg gctgctgtag atgacgaggt
     1141 gcactatgga aacatcctca ccgtaccagc ctactgtgta ggggacattt ctcaccacat
     1201 agcccaatcc acctacaaca tgtgcaaaga cgatgtggta atgggagtta ttgaggccac
     1261 cactgcagtt atggaatcca ccctccaagc cgcactaccc aacttccaaa gcgtggtaga
     1321 aatattatca ctggccacag ggtcatcagc ctgtgccgtg gaatatatac tggaactgga
     1381 tgggtttaac gcaccaatgg tggtggatct gctcactaaa aagttccaca acttcgttca
     1441 actctaccca accaggggtg cggcagctga attgcacaac tgtgacttca tggacatgat
     1501 ataccgggga tggaaccact tggatgcggc tcgacgagac caaaacggct cacccggaaa
     1561 actcatacca cgaataggaa acttcgccgt ggatctggaa cccgtacacc agaacgaagt
     1621 tatcatgaac ccacaacgct acacctaccc tgcctgtgct ataacagtcc gtttttcagc
     1681 attaatgcgc ctggcggact acccctgctt gttaaccagt gaaccagtta ctgctactct
     1741 gatgactaac atcatagcct taaacaagaa gaacccagca tcaccagctc gtacttgtaa
     1801 aaaatgtgca tctgcctgtc tggttgattt ccgccacagc cattgcgaat ggaaggaagc
     1861 agtttaagaa tgaaccccta tggatgctga aaatatgaaa tgttacatat gcgcccagga
     1921 gggaaaggat tccgatgccc tggcgatctg catcgtctgt ggtatgggcc tctgccagga
     1981 acacatcatt caccaggaaa tagagctatg ggaaggtgga tacccattcc catctgagaa
     2041 gattaaggcg actcttcccc gtatactctg cccatactgc tataaagctc tgaaggggga
     2101 ctaatagatg gcatctctgg ctaaacgggc cattgctgaa ctggtaggta ccttcctact
     2161 ggtttttttt ggtaccggag cagcgatcat caccctgatg atcagtgctg gtcaggaacc
     2221 cgccaccacc tttaacattg ggataggtgt gttggggggt cttggtgatt ggctggccat
     2281 tggtctagcc ttcggatttg ccatcatggc ctcaatttat gcattgggct ccatttctgg
     2341 ctgtcacatc aacccggcgg ttaccattgg tttatgggcg gttaaaaagt ttcccagtca
     2401 ggatgtactg ccctacatca tagcacagtt gataggggcg tccctggcca gtttaaccct
     2461 tgtgatggct ttgggtatgg atgcagttac cattggtggt ttaggggcca cagccccatt
     2521 ccctggtgta ggttattggc aggccattgt ggtggaggcc attggaacct tcattctagt
     2581 gttgaccatt atgggagttg cagtagatcg cagagccaca cccggatttg caggactggc
     2641 cattggttta actgtggctg gtgttatcac caccgtgggt aacataacag gggcttcact
     2701 caaccccgcc aggaccttcg gcccatacct gggagacatg ttactgggtg gaagtaactt
     2761 gtggaattat ttccccatct acgttgttgg gcccattgta ggtgctgtgg ttgctgcact
     2821 tctctaccag tatctcatca gcgaagacac caccctctga tcaacagagg acccaaaaaa
     2881 aacattcaat ctttttttat ttttttttat tctattttaa tcacttctga taatggatgc
     2941 agggagaaac caccctacca gtgtggtcaa tgacaacacc ctcattttct agtaaagtcc
     3001 ttttcattat ggttccgcct tgaaaaccac ccagatgttt atctgagcga attattcgat
     3061 gacagggtat tataatggga aagggattag ttgccagggc atttccaacg gctcgggcgg
     3121 cttgtttgtg gcccacatgt gctgcaatga atttataggt actgacacat cccctcggaa
     3181 tcaggtaatc agcaaccagc actctcttct ggaagggtgg acacgtatct aaatttacca
     3241 tatccaggga aaacttcaca tcttctcctt gaagatacgc actgattttt gcggcaacag
     3301 catcaatttc cttacaggat gatggtttag ccttggggtt gatgttgttt acctgttcta
     3361 tagtagaata acctggtttg gaaatgagaa cccggttgat ctggggatga tcatcgacta
     3421 tggaccacag aattgccact ggcccaaagg gagttgtttt tatgatttta gatttcatat
     3481 ccttctacat tcactttttt tagttggaat catcaaaagg gctgtaaatg gtgattggga
     3541 tcaggggtta tagattaatt taactcacca tatactacta ccaacacttg aaaaaggtta
     3601 taataccttg ttttcaccct acaaacatta aataacactg tttcattctt aaaggttttt
     3661 attctctaca tctgtctatt tcacgcgttt ttattgctga aattttttca accaaccttc
     3721 acaaagatgc cctgaatcca tttttattga tgtagggaat actttcatct gataggtatt
     3781 ctatgcatca gtattgtcat attttagctg cacagtacat tacagcccct gatgatctac
     3841 caccacggga tatgaagctt ccatccaagc ctccatactc cccagcacac tacccacatt
     3901 ttgaaaacct tttcgaagaa gataactggc caccatggtg gatttgttcc ctgaatcaca
     3961 gtatataaca atttcctgat ttttggggat atcattcaat tgctgtggaa ctaaccctgc
     4021 atatatatga tttgaacctt caatgaaacc tttttcacga tcaaccaact tacggacatc
     4081 taataggtaa aaatcaccat caaactgccg tttccttagt tcctgtacag tttgaagttt
     4141 taaagcttct gtttcgtgtg cttgagtata ccaggatgaa aatcctccgg aaagatac
//
LOCUS       DAOQVW010000069         4143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_24106_length_4143_cov_3.271771, whole genome shotgun sequence.
ACCESSION   DAOQVW010000069 DAOQVW010000000
VERSION     DAOQVW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24106_length_4143_cov_3.271771"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1746
                     /gene="atwA"
                     /locus_tag="PKK85_02365"
     CDS             151..1746
                     /gene="atwA"
                     /locus_tag="PKK85_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HNS24897.1"
                     /translation="MSFIELENVTKSFDGVKVLKNLNVKIEEGEVLGILGKSGAGKSV
                     LINMLRGMKDYRPDEGSIKYTVAFCPSCFRVEAPSYDGKECGCGCDFQVQTVDFWNCD
                     RLIFAAIKRRISIMLQRTFALYEDDTVIDNVLKSIEGHEEEESTYMAIELLDMAQMTH
                     RITHIARDLSGGEKQRVVLARQIAKEPMIFLADEPTGTLDPKTAEAIHDALIEGVVQQ
                     GTTMIITSHWPEVMKKLSTQVIWLEKGEVVEEGDPETVVQKFLDQVPLPEKTEEFKAG
                     GPIVRMEGVKKHYYSIERGVIKAVDGVSLTVDEGEIFGVVGLSGGGKTTLSRILYGLT
                     DPSSGMIEIRLGDQWIDMTEKGPFGRGRVKPYLGILHQEYSLYPHRNVLGNLTEAISL
                     DLPAEFARMKAAYVLNAVGFDENYAENILKKYPDELSGGERHRVAIAQVLIKEPNVVI
                     LDEPTGTMDPMTRVQVTDSIHKAREELNQTFLIISHDMDFVLHVCDRAALMRGGKILK
                     QGKPEDIVEDLSPSEKEKMLKED"
     gene            1748..2224
                     /locus_tag="PKK85_02370"
     CDS             1748..2224
                     /locus_tag="PKK85_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HNS24898.1"
                     /translation="MAWADSPSHVCRGGDKRALTFCCPPVKPCPILHTLEDAKITPQE
                     YVDIKEDFGRKTRLGHGEGTCFGSLVWCCKPSKPCPLRDMVMRRINMSNDEYLELKKE
                     LSEKLVGKTESSAEEKVKGLAEAFNVSEDEARQTLQECGNDLRMAMKLLQMKNLEQ"
     gene            2226..2882
                     /locus_tag="PKK85_02375"
     CDS             2226..2882
                     /locus_tag="PKK85_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNS24899.1"
                     /translation="MGWTPLFLDMTDWHVLVIGAGEVGERRARRFLDADAQVIITGGR
                     VSDDLKSKGALEKPLHQWEELVDWADLVVVASGDKALNQEIGEMSAGKLLNRADKPEY
                     GNMIVPSSFFIGDVQICIFTQGKSPLMARQLRKKIQKVINQEDVLQLELQHFTREKLK
                     GQLHNQKDRRQLLYQILDDEYIKKLLAEGNLLAAQERVAHIIELDSESKPTRSNEEIS
                     "
     gene            2879..4069
                     /gene="hemA"
                     /locus_tag="PKK85_02380"
     CDS             2879..4069
                     /gene="hemA"
                     /locus_tag="PKK85_02380"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNS24900.1"
                     /translation="MILNIRIDHKTAEISQMEQLNQHMEGIFQEINQNHSVNEYLQIK
                     TCNRQEMYLVIDKCDLNYHWGDLVVKKDDEALEHVLRLASGLESMIVGEDQILGQLRD
                     AQKQAIKAGSCGPILNNVFTKAIHVGQLVRKKTHINEGAVSIGSAAVRLAEHVHGDLK
                     CKKVLVIGAGRMGTLVAKALVVKRLKAIVVANRTHDRALCLAKELGGSAIYFDRLAEA
                     LYDADVVISATGAPHPIITYEKLKEAVPVETLGKMVMVDIANPRDIDERVAELGVRLY
                     NIDDLRGIARENLQMRENEAKAAEAIIGEELVLLRRSLKHLEVEPLISSIRNQAEKMR
                     IKETKKALRMMGDINGQEKVVDDLSRVVVDRIFSGIIKNIKIAAEKDDKSTLEVVERL
                     FSNG"
BASE COUNT         1337 a          756 c         1034 g         1016 t
ORIGIN      
        1 tagccagggc agctattgga gggagtttga actttgaact ttcacggata taaacgtcaa
       61 agtttataat agaaatgatt agtaaatatt atttatataa tcacgaactg attagaatat
      121 atattcatta taaatcatca aggtggttgc atgtctttta tagaactaga aaatgtcacc
      181 aagtcttttg atggcgtgaa ggtcctaaaa aacttaaatg tcaaaataga ggaaggagaa
      241 gtactaggca tcttgggaaa gagtggtgca ggtaaatccg ttcttattaa catgttaagg
      301 ggaatgaagg attatcgccc agatgaaggt agcataaaat ataccgtagc attttgtccc
      361 agttgtttcc gggtggaagc accttcttat gacggaaaag aatgcggatg tggctgtgat
      421 ttccaagtac aaactgttga cttctggaat tgtgaccgat taatattcgc tgccattaaa
      481 agaagaattt ccataatgtt acagagaaca ttcgcccttt acgaggacga tactgtaatt
      541 gacaatgttt tgaaatcgat agagggacat gaagaagaag aaagtaccta catggccata
      601 gaacttttag atatggctca aatgactcac cgcatcaccc atatcgcccg ggatctcagt
      661 ggaggagaaa aacaacgggt agttctggcg cggcagatag ccaaggagcc catgattttc
      721 ttagctgacg aaccaaccgg taccctagat cctaaaactg ctgaggctat acacgacgct
      781 ctcatcgaag gagtggtgca acagggaacc accatgatca ttacctctca ctggccagaa
      841 gtgatgaaaa aactttccac ccaagttata tggttagaaa agggtgaagt agtagaggaa
      901 ggggaccctg aaaccgtagt gcagaaattc ttggatcaag tacccttacc tgaaaaaacc
      961 gaagaattca aggccggagg acctatagtc cggatggaag gagttaaaaa acattactac
     1021 tccatagaaa gaggagttat taaagctgtt gatggtgtga gcctcactgt tgatgaaggc
     1081 gagatatttg gggtggtcgg tttaagtggt ggtggaaaga ccactttatc acgcatactg
     1141 tacggtttaa ccgatcccag cagcggtatg attgagatcc gcctggggga tcagtggata
     1201 gatatgactg agaagggtcc ttttggtagg ggaagagtga agccgtactt gggaatactt
     1261 caccaggaat acagcctata cccacatcga aacgtccttg gcaaccttac cgaagccatc
     1321 agtctggatt tacctgcaga attcgccagg atgaaggctg cttatgtctt aaatgcggtt
     1381 ggatttgatg aaaactatgc tgaaaatatt ttaaagaaat atcctgatga attaagtgga
     1441 ggggaacgcc accgcgtggc aatcgcccaa gttcttatta aagaaccaaa tgtagttatt
     1501 ttagatgaac ccaccggtac tatggatccc atgaccagag tacaggtgac tgattccatc
     1561 cacaaagccc gggaagaact taaccaaacc ttcctgatta tatctcacga catggatttc
     1621 gtgctgcatg tatgtgatcg ggccgcactg atgagggggg gaaagattct caagcaagga
     1681 aaacccgaag acatagtgga agatctgagc ccaagtgaaa aagaaaagat gctaaaggag
     1741 gattaggatg gcctgggctg attcgccgtc gcatgtatgt agaggagggg ataaaagagc
     1801 attgaccttt tgttgtcccc cagttaaacc ctgtcccatc ttacacaccc ttgaagatgc
     1861 aaagataact ccacaagagt acgtggacat caaagaagat ttcgggagaa aaactcgact
     1921 aggtcatgga gagggaactt gcttcggatc cctggtgtgg tgttgtaaac cttccaaacc
     1981 ctgtcccctg cgagatatgg taatgcgcag gatcaacatg tctaatgatg aatatttaga
     2041 actgaaaaaa gagttatccg aaaaattagt ggggaaaact gaatctagtg cagaagaaaa
     2101 ggttaaagga cttgcagaag cctttaatgt ttcggaagat gaagcccgtc aaaccttaca
     2161 ggagtgtgga aacgacctta gaatggccat gaaacttctt caaatgaaga atttggagca
     2221 ataggatggg atggactcct ttattcctgg atatgacaga ttggcacgtg ctggtgattg
     2281 gtgctgggga agtgggagaa aggagagcca gaagattcct ggatgccgat gcacaagtca
     2341 tcataactgg tggaagagtt tctgatgact taaaatccaa gggtgccctg gagaaacccc
     2401 tccaccagtg ggaagagctg gtggactggg cggacttggt ggtggtggcc agtggcgaca
     2461 aagcactgaa ccaggaaatt ggggaaatgt ctgctggaaa acttttaaac cgggcagata
     2521 aaccagaata tggaaacatg atcgttccat cttccttttt tataggagat gtgcagatat
     2581 gcatttttac ccagggaaaa agccccctca tggcccggca gttaagaaaa aaaatacaaa
     2641 aagtaataaa tcaggaggat gtactgcagt tagagttaca gcatttcacc cgggaaaaac
     2701 taaaaggcca actccataat cagaaggatc gtcgccagtt actataccag atactagatg
     2761 atgaatatat taaaaaacta ttggcagagg gaaatttgtt ggcagcacaa gaaagagtag
     2821 cccacattat agaattagac tctgaatcta agcccaccag gtccaacgag gagatatcgt
     2881 gattcttaat atcaggatcg accataaaac agctgaaatc agtcagatgg aacagttgaa
     2941 tcagcacatg gagggtattt ttcaggaaat taatcaaaac cactctgtga acgagtatct
     3001 ccagataaaa acatgtaatc gccaggaaat gtatctggta atagataaat gtgatctaaa
     3061 ttaccactgg ggcgatttag tggttaaaaa agatgatgaa gccttagaac atgttttaag
     3121 gttagcatct ggcttggaat ccatgattgt tggtgaggac cagattctgg gtcagctcag
     3181 ggatgctcaa aaacaggcca ttaaagcagg cagttgtggg cccatactaa acaacgtgtt
     3241 taccaaagcc atccacgtgg gccagcttgt ccgaaaaaaa actcacatca atgaaggggc
     3301 agtttccatt ggatcagcag cggtgaggct ggctgaacat gtgcatggtg atcttaaatg
     3361 caaaaaggtg ctggttatcg gagctggacg aatgggcacc ctggtggcta aggccctggt
     3421 agttaaaagg cttaaagcta tagtagtggc taaccgtacc catgatcggg cattgtgcct
     3481 ggcaaaggaa ttgggaggtt cagctatcta ttttgatcgt ttagcagaag ctctgtatga
     3541 tgctgatgta gtgatcagcg ccactggagc accacacccc ataataacct atgaaaagct
     3601 caaggaagct gttccagtag aaaccctggg taaaatggtg atggtggata ttgctaatcc
     3661 ccgggatatc gatgaacggg tggcagaact aggagtacgg ctttacaata ttgatgatct
     3721 gaggggaatt gcacgtgaaa atcttcagat gcgagaaaat gaagctaaag ctgctgaagc
     3781 tattattgga gaagaattag ttcttctaag aagatccctt aaacacctgg aagtggaacc
     3841 actgatctca agtatccgca accaagcgga aaaaatgaga atcaaagaaa caaaaaaggc
     3901 cttgagaatg atgggggata tcaatggcca ggaaaaagtg gtggacgact tgagtcgggt
     3961 tgtagtggat cgcatattct cgggtatcat caagaatata aaaattgccg cggaaaaaga
     4021 tgataaaagt actttagaag ttgttgagcg cctttttagc aatggataaa tttaaacttt
     4081 tgacatagaa aaaaatgaga tgttaagcat acatctcttt gggctctttt ttatcttttt
     4141 cat
//
LOCUS       DAOQVW010000070         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_24160_length_4135_cov_3.764706, whole genome shotgun sequence.
ACCESSION   DAOQVW010000070 DAOQVW010000000
VERSION     DAOQVW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24160_length_4135_cov_3.764706"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..986
                     /locus_tag="PKK85_02385"
     CDS             81..986
                     /locus_tag="PKK85_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24901.1"
                     /translation="MEDIKEIKSVRIVPYTLMSSSVSAVLGLLYAIVLLIVLGIIALV
                     VPGVGQYATILASLGVALIITMPVLNFFFSILSSFVKALIYNLLVPRIGGIKVSMDGD
                     EVKSIPIVPVALMLSILYTVLAFIVMLVVAPILAVFMEGTTLIAGSTAAAIPELSGMA
                     GASGAGIILAIILIIGVPIIFFITTFLYAVVATILYNLLSPIIGGVKLKMEQASHGLC
                     EIRKIPPVSLALILSVVLTIVNLLYQLPSAAMNIALDGVAIGLVGLLGSIVVYFIVFF
                     IVYILMALIYNFLQPKIGGVEIKLE"
     gene            1033..1452
                     /locus_tag="PKK85_02390"
     CDS             1033..1452
                     /locus_tag="PKK85_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HNS24902.1"
                     /translation="MKRTLKCRRISRGKAKGQVIVTSEPISFLGGVDPATGRVIDREH
                     ELYQQNLKDKILVIPSGKGSTVGSYVIYQMAKNQTAPRGIVAMEAEPIIATGAIMAGV
                     PMVDHPEVDILSILKNGDLMELDADSEIIKIINKQFY"
     gene            complement(1446..1832)
                     /locus_tag="PKK85_02395"
     CDS             complement(1446..1832)
                     /locus_tag="PKK85_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HNS24903.1"
                     /translation="MEWKYVVVGGLITAVLTLVLAVVFLPLFFLGPVIGGVFAAYLDR
                     GDNQAGAVEGAVSGVIGGLIMGALFLLGFGTISAIIGVIFTNAGAAAGTIGVFLGAFI
                     TLLAVIVSGVLGAIGGVVGVSIRENQ"
     gene            complement(1878..2273)
                     /locus_tag="PKK85_02400"
     CDS             complement(1878..2273)
                     /locus_tag="PKK85_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24904.1"
                     /translation="MEFNYKGLAIGLGVTLVWYAFFIFVEGYGAAVIAPLVGGYASNN
                     YLKGKYREGIIYGGLSGVTGAFLTAVVLSLCHFNALETMGYTDIGTISVELTLVLAII
                     AGLSMGIAGGLVAGLSFKRRETGQKPQTK"
     gene            2460..3707
                     /locus_tag="PKK85_02405"
     CDS             2460..3707
                     /locus_tag="PKK85_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS24905.1"
                     /translation="MGYILIHNGTVIDGNGGQPVQDGGLLIKDNVVQEVGSLDSVRLP
                     PHDVEEIDANGGFILPGFIDCHVHIMLNGFRFEDPLFTPLSYYFFQAGKNLRQTLEAG
                     VTTVRDAGLADYGVKKAVDDGLIPGPRLQISVMPLSITGGHFDLCMKSGIEVKTSYSG
                     LPDSVCDGAHEVRKRVREVLRAGADVVKVMVTGGVMSPNDSPEHPQFSREELGVMVEE
                     ASYRGICLMAHAHGAQGIKNALKAGITSIEHGTFLDEECLDLLLEKDGWLIPTLLVNQ
                     HNQEMAEKGKLPEYSHGDSQEVARILERNMKIAYQAGVKMAMGTDSGIAPHGQNLREL
                     SLLCGVGMTPMEAIQAGTIRAAELLGWDHKIGTLEQGKLADVVVCKDDPLDRIDSLGD
                     PENINLVLKAGKLVKDLRHEKVE"
     gene            3707..>4135
                     /locus_tag="PKK85_02410"
     CDS             3707..>4135
                     /locus_tag="PKK85_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNS24906.1"
                     /translation="MAESKKKAEIKISGMHCASCALNVEKFLQSVEGVENAQVNFGTE
                     KATVEYDPEKVELVKIEEKVQDAGYGVVNEQVTLKIGGMTCAMCVQAIEDVLNRIDGV
                     SQVMVNLASEKAYVTYNPLMTSVSQMRDAIEGLGYEYLGME"
BASE COUNT         1165 a          926 c         1007 g         1037 t
ORIGIN      
        1 catcgctata atcggttagc aggggaacta ttatatatta ccttgacaat ccactttata
       61 aaaaaaggga gtacgttaaa atggaagata taaaagaaat aaaatcggta cgtattgtac
      121 cctacacact gatgtcctca tctgtatcag cagtgttggg gctgctatat gccatagttc
      181 tattaatagt tcttggaatc attgccttgg tggtgccagg tgtgggtcag tatgctacca
      241 ttctggccag cttgggggtg gccctcatca tcaccatgcc tgttttaaac ttcttcttca
      301 gcatactgtc atctttcgtc aaggccctta tctacaatct gctggtgccc cgtatcgggg
      361 gaataaaggt gagtatggat ggggatgaag ttaaatccat ccccattgtg ccggtagccc
      421 tgatgctgtc catactctac accgtgctgg ctttcattgt gatgctggtt gtggccccaa
      481 ttttagcggt atttatggaa ggaactacct tgatagccgg cagcaccgcc gcagccatac
      541 cagaattatc aggaatggct ggtgccagtg gtgcagggat tatattggcc atcatcttaa
      601 taatcggcgt tcccatcatc tttttcataa ctactttctt atatgcagtg gtagccacca
      661 tattatacaa tctcctgtcg cccataatcg gtggagtgaa gcttaaaatg gaacaggcca
      721 gtcatggctt atgtgaaatt agaaaaattc caccagtatc tctggccctc atcctatcag
      781 tggtgttgac tattgtgaac ctcttatacc aactgccctc tgcagccatg aatatagctt
      841 tagatggagt ggccattggc ttggtaggtc tgttggggag tatagtagtc tatttcatag
      901 tcttcttcat cgtttatatc ctgatggctc tgatatacaa cttcctgcag ccgaaaattg
      961 gtggagtgga gattaaactg gaataaaatg gatctacacc gggagttcca tttccaatct
     1021 ttgaggttaa ttatgaaaag aaccctgaag tgccggcgca tatccagagg aaaagctaaa
     1081 ggtcaagtaa tagtcaccag cgagccaatt agtttccttg gtggtgtgga tcccgccact
     1141 ggcagggtta tagaccggga gcatgagctt taccagcaga acttgaagga taagatcctg
     1201 gtaatcccat cgggtaaggg ctccacagtg ggttcctacg taatatacca gatggccaaa
     1261 aaccaaactg ccccccgggg gatagtggcc atggaagctg agcccataat cgctaccgga
     1321 gctataatgg ctggtgttcc catggtagac catcctgaag tagatattct gtctatcctg
     1381 aaaaatggag atttaatgga actcgatgcc gattctgaaa tcataaaaat aataaataaa
     1441 cagttttact gattttcgcg gatgctaaca cccaccactc cgccgatagc acccagaact
     1501 cccgaaacaa tcactgccag gagggtgatg aaggctccca ggaaaacacc gatggtacca
     1561 gctgctgctc ctgcgttagt gaaaataaca ccaataatag cgctgatagt accaaatcct
     1621 agtaaaaaca gggcccccat aattaatcca ccgatcacgc cggaaacagc tccttccacg
     1681 gcgccagctt gattgtcgcc gcggtccagg taagcagcga aaacgccccc tataaccggg
     1741 cccagaaaga acaggggcag gaataccaca gccagaacaa gggtcagaac cgcggttata
     1801 agtccaccca ccactacgta cttccattcc atattatcac ctttacatat aatcctacat
     1861 attgaattaa ggaatattta ttttgtttgc ggtttctgtc cagtttcccg ccttttaaag
     1921 gataatcctg ccaccagtcc accggcgatg cccatagaca aaccggcaat tatggccaaa
     1981 accagggtta actccaccga aatggttcct atatcagtgt agcccattgt ctccagtgca
     2041 ttgaaatgac agagagacaa aaccacagcg gttaaaaaag ctcctgttac accgctgagg
     2101 ccgccgtata taatcccttc ccgatatttt cctttcaaat agttgttgga cgcatatccc
     2161 cccaccaagg gggcgataac tgccgcaccg tagccttcta caaatatgaa aaaggcgtac
     2221 cacactaaag ttacacccaa accaatagct aatcctttgt aattgaattc catttatttc
     2281 cctcaccacg acataaattt gaagttacta tcacaccact ctactatctt ctacatcatg
     2341 atccttaaaa aggatattaa aagccgagat tagatttctc tattctatag cataaaatga
     2401 tgtagtagaa gcaaataagt tgtatagttt accatagtac tatcctaagg tgatgatcaa
     2461 tgggatatat tttaattcat aacggaacag tgatcgacgg caatggcgga caaccagtgc
     2521 aggacggtgg attgctgatt aaggataatg ttgtccagga ggtaggttcc ctggattcag
     2581 ttaggctacc gccccatgat gtggaggaaa ttgacgccaa tggggggttc atactccccg
     2641 gttttattga ctgccacgtg cacatcatgc tcaatggttt ccggtttgaa gatcccctat
     2701 tcacacctct ttcttactat tttttccagg ccgggaaaaa tctccgccag accctggagg
     2761 caggggtcac cacggtgcgg gacgcgggcc tggctgatta tggagttaaa aaagcggtgg
     2821 atgatggctt gatacccggc cctcgccttc agattagtgt gatgcctctc tccattacag
     2881 ggggacattt tgatctatgt atgaagtccg ggatagaggt aaaaaccagt tacagtggcc
     2941 tgcctgattc tgtttgtgac ggagcccatg aggtccgtaa aagagtacgg gaagttctaa
     3001 gggctggcgc agatgtggtg aaggtcatgg ttacaggtgg tgtaatgagc cccaatgaca
     3061 gccctgaaca tccccagttc agccgggaag aactgggagt catggtggaa gaggcctcat
     3121 atcgaggaat ttgtttgatg gcccacgccc atggagcaca aggtataaaa aacgctttga
     3181 aggcaggaat cacctcaatt gaacatggta ccttcctaga tgaggagtgc ctggatttgt
     3241 tgttggaaaa agatggctgg ctgattccca cccttctggt caatcagcat aaccaggaga
     3301 tggctgaaaa aggaaaactt cctgagtaca gtcatggaga ttcccaagag gtggccagaa
     3361 tactggaaag gaatatgaaa attgcctacc aggcgggggt gaagatggcc atgggtaccg
     3421 actccggaat agccccccac ggtcagaatc tgcgtgaact atccctgtta tgtggagtag
     3481 gcatgacccc aatggaggcc atacaagcgg ggactatacg tgccgcggag ttactgggct
     3541 gggaccataa gattggaacc ctggaacagg gaaagttggc tgacgtagtg gtgtgtaaag
     3601 atgatccatt ggatcggata gactccctag gagatccaga aaatataaac ctggttctaa
     3661 aggccgggaa attggtgaag gaccttcgcc atgaaaaggt ggaataatgg ctgaatctaa
     3721 gaaaaaggca gagataaaga tttctggaat gcattgcgca tcctgtgccc ttaatgttga
     3781 aaaattcctc caaagtgtgg aaggagtgga gaatgcccag gtgaacttcg gaaccgagaa
     3841 ggccaccgtg gaatacgacc cggaaaaggt ggaactggtc aagatagagg aaaaagtcca
     3901 agatgcaggg tatggtgtgg tcaatgaaca agtaaccctg aagatcgggg gcatgacctg
     3961 tgccatgtgt gtacaggcca ttgaggatgt gctgaaccga attgatggtg ttagtcaggt
     4021 aatggttaat ctggcctctg aaaaggccta tgtaacctac aatccactga tgaccagtgt
     4081 ttcccagatg cgagatgcca ttgaaggtct gggatacgag tacctgggaa tggaa
//
LOCUS       DAOQVW010000071         4111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_24381_length_4111_cov_3.446499, whole genome shotgun sequence.
ACCESSION   DAOQVW010000071 DAOQVW010000000
VERSION     DAOQVW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24381_length_4111_cov_3.446499"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1166)
                     /locus_tag="PKK85_02415"
     CDS             complement(156..1166)
                     /locus_tag="PKK85_02415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS24907.1"
                     /translation="MECLESLFWVDYPCFDVVVVDNASEDDSLLRIREYCQGDLVVGS
                     AFFTYNSGNKPIQVTELTEKQVRSKMREKVDRPKSSCQAPEARNHLTLIKNDVNHGFA
                     QGNNIAMEYALAVFDPDYLLLLNNDTVVDPHFLTEMVNVTVGEGVGMVGPKILKATDP
                     RVIDSAGHIISWGRIVDRGHGEVDEGQYDQDINVIGVMAAAALYKREMLMDIGFLDSS
                     YVTLGEDADLSWRAYNNGWRAVFAPKAVVYHKRGQSITKESVLPRMTILSTKNTTKYV
                     VKNGKNRHKFLYLFVLFKEGLFVLAGSVLGKNNVNTLQYSSVLLNSYLTIFKSFFQLR
                     LS"
     gene            1578..2396
                     /locus_tag="PKK85_02420"
     CDS             1578..2396
                     /locus_tag="PKK85_02420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS24908.1"
                     /translation="MEKKPELSVIILNYKNPELTAKCVNHLIEASNGANIRTETIIVD
                     NSAPETASTLRNLLPGEVVIIENERNQGFSKANNQGISISQGEYVLLLNNDAFINAEV
                     LKTGISFLRENPDCGMWAPKLVGEDGTFQMTCARLPSLKGLIVEYLLFRSYDRYSESE
                     SWTEPQVVEAVIGAFMLISRRVVEVIGMLDEDFFFTVEDIDYCFRVNEANFKIYYDPT
                     VSMIHLVSASQKEGIEYPKLHSNRILYFKKNKGLFLGLISGIIINMGLFLRKIL"
     gene            complement(2458..>3044)
                     /locus_tag="PKK85_02425"
     CDS             complement(2458..>3044)
                     /locus_tag="PKK85_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNS24909.1"
                     /translation="HEALKLSYQKSFKYLALLSIPVSVGTTVLAPEIVLLIYKSAFTP
                     SIIILQILIWAIPITFLNYIFGTILPAMNRQNMLLKVTFMAMILNIALNLVFIPTYSY
                     VGAAVVTVFTELFIFILCLLILSRTFSKVRIQEVFFKPVVASLIMLLFLLYFKTNLFL
                     EIALGAIIYFIVLIAIRTFQDDDWDILRQIMGRK"
     assembly_gap    3045..3054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3055..3854)
                     /locus_tag="PKK85_02430"
     CDS             complement(<3055..3854)
                     /locus_tag="PKK85_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNS24910.1"
                     /translation="MSRVRKIAKNTTMLFVSQIITYIMGFFITMYSARYLGAEGFGII
                     SLALAITGILVVFTDLGLGTLTVREVARDKSLVDKYTSNVAVIKLLLAFFTFILTAVI
                     VYLFGYADEIKLVVYIITGSTLVNALSGIFYPIFQSYEKMEFQSIANVLNSSIMLIGT
                     FFVIFYNLGIYSFATLYLASNIVVFFFIVSVYIREFNSPSFNVDFAFWKPTLMMALPL
                     SLVSIFSLIAYRVDTILLSLLKGSMVVGWYSASYRLMEIFLFLPGVFAT"
BASE COUNT         1236 a          829 c          838 g         1198 t
ORIGIN      
        1 acgactccaa acactccaca gtatcctccc aaccattcca attaagaaca actaccgtaa
       61 ctttaggatc catggttttt ctccaaattt aatcagttct agaaaagtta tctgcattat
      121 ggctattaat tatcttgtgg gttaacgttt cttagtcaag acaatctcag ctgaaaaaag
      181 cttttaaaga ttgttaagta ggaatttagg agtacactgg aatattgaag ggtgttcaca
      241 ttatttttcc ccaagacact cccagccaat acgaagagcc cctctttgaa cagtacaaag
      301 aggtatagaa atttgtgacg gttttttcca tttttaacca cgtacttagt ggtgtttttg
      361 gtacttaaaa tggtcatcct ggggagcact gattctttgg tgatgctttg gcctctttta
      421 tgatacacta ctgctttggg tgcgaataca gctctccatc cattgttgta tgctctccat
      481 gataaatcgg cgtcctctcc cagagtcacg taactactgt ccaggaaacc tatatccatg
      541 agcatttccc ttttataaag tgcagctgcg gccatgactc ctataacatt aatatcctgg
      601 tcgtattgtc cttcatcaac ttctccatgt ccccgatcca cgattctgcc ccagctaatg
      661 atgtgtcctg cagaatctat tacccgggga tcagtggcct tcaatatttt cggccccacc
      721 attcccaccc cctccccaac tgttacatta accatttcag tcagaaagtg gggatcaacc
      781 acggtgtcgt tgtttaaaag aagcaagtaa tctgggtcaa acactgccag ggcatactcc
      841 atggcaatat tgttaccctg tgcaaagcca tggtttacat cgttttttat gagggtgaga
      901 tgattcctgg cttctggcgc ttggcaggag gatttaggac gatctacttt ttccctcatt
      961 ttgctacgga cctgcttttc agttaactcc gtgacctgga tgggcttgtt cccactgttg
     1021 taggtgaaaa aagccgaacc caccaccaga tccccctgac agtactccct gatccgcaga
     1081 agagaatcat cctccgaagc attatccacc accaccacat caaaacaagg ataatcaacc
     1141 caaaacaacg actccaaaca ctccacagta tcctcccaac cattccaatt aagaacaact
     1201 accgaaaccc ggggtgccat agtatcacat agattttaca ttattttctt tattaccggt
     1261 gctggcattt tagagtaggc cattttcaaa tggattttga ttcaactaag tggctgtttg
     1321 aatctttatt ggtcactttc atttattatt tatcaggatg gggataggat gagtccaacg
     1381 attccgggct gatatatgct taatggccca tttatcttct gatagagccc tgttgaaggt
     1441 aatgttggat tgatttgatt tttggtgtgt acgtggcatt tggaaacctg attgatggtt
     1501 catttttatt gtggtggtac ttcaaatgtt tatgaaaaag gtggtattaa ttagtaggat
     1561 gatgggagaa ttagtctatg gaaaaaaaac ccgaactatc ggtaatcatt ctgaattata
     1621 aaaacccgga attaacagcg aaatgtgtta accacttgat tgaagcttct aatggggcga
     1681 atatccgtac cgagaccatt atcgtggaca acagtgcacc ggaaactgcc agtaccctga
     1741 gaaatttgtt gccaggggag gttgtaatca tagaaaatga aaggaaccag ggtttttcca
     1801 aggccaacaa ccagggtatc agcatcagtc agggagaata tgttctacta ttaaacaatg
     1861 acgcctttat caatgctgaa gttttaaaga ctggtatcag ttttttaagg gaaaatccgg
     1921 attgtggaat gtgggctcct aaactggtgg gggaagatgg tacattccaa atgacctgtg
     1981 ctcgattacc atcccttaag ggtttgatag ttgaatattt gctatttaga agttatgatc
     2041 gttattcaga atctgaatca tggacagagc cccaggtggt ggaggcagtt atcggtgcct
     2101 ttatgctcat cagtagaagg gttgtagagg taattggaat gcttgatgaa gatttcttct
     2161 ttacagtgga agacattgac tactgcttcc gagttaacga ggccaacttc aaaatatact
     2221 acgaccccac cgtgagtatg atccacctgg tatctgcgtc acaaaaagag gggattgagt
     2281 atccaaaact ccattcaaat cgtattttat acttcaaaaa aaataaaggc ctatttttag
     2341 gcttaatttc tggaattatt attaacatgg gacttttttt aagaaagatt ttataaaata
     2401 tccttatttg caaccaccaa atttcatatc aattattccc ccatctaact atatctccta
     2461 ttttctcccc attatctggc gcaaaatatc ccaatcatca tcctggaatg tgcgtatagc
     2521 aattaaaact atgaaataga ttatcgcccc cagggcaatc tccagaaaca agttggtctt
     2581 gaagtacaat aggaaaagta gcatgatgag gctggccacc accggtttaa aaaagacttc
     2641 ctggattctt actttactga aggttcggga gagaataagc aggcataata tgaagatgaa
     2701 cagctcagtg aacacggtta ccactgccgc gcctacgtag ctataagtgg ggatgaatac
     2761 caggttcagg gcaatattta aaatcatggc catgaatgtt accttcaaaa gcatgttttg
     2821 acggttcatg gccgggagta tggttccaaa gatataatta aggaaggtga tgggtatggc
     2881 ccatatgaga atttgcagaa taataataga cggggtgaag gcacttttat agattaataa
     2941 tacgatttcc ggagctaaaa cagtggttcc tactgatact ggtatggata acagggccag
     3001 gtatttgaag gacttctggt aggatagttt caaggcttcg tgtgnnnnnn nnnngtggca
     3061 aaaactcctg gtagaaatag aaagatctcc atcaaacgat aagatgcact gtaccacccc
     3121 actaccatgc taccctttag tagggagagg aggatggtgt caacccggta ggcaatcaac
     3181 gaaaatatgc ttaccaggga tagtggcagt gccatcatta aagtaggctt ccagaaggcg
     3241 aagtcaacgt taaatgatgg ggagttgaat tctcggatat ataccgagac aatgaagaaa
     3301 aaaaccacta tattagatgc cagatagagt gtggcaaagg agtagatgcc taggttataa
     3361 aaaattacga aaaaggtacc gatgagcatt atggaactgt taagtacatt ggctatggat
     3421 tgaaactcca ttttctcata ggactggaag atgggataaa atatcccgga aagggcattt
     3481 acaagggtgg atccagtgat tatgtaaacc accaatttga tctcatctgc atatccaaaa
     3541 aggtaaacga taactgctgt taaaataaag gtaaaaaatg ccagcaggag ctttataacc
     3601 gctacgttac ttgtatattt gtctaccagc gatttatccc gggctacttc cctaactgtg
     3661 agggttccca gtcccaggtc agtgaatacc actaaaatac cggtaatggc cagtgccagg
     3721 gatatgatgc cgaagccctc tgcacccagg taacgggcac tgtacatggt tatgaaaaaa
     3781 cccattatat aggttataat ctgggaaaca aacaacatcg tagtgttctt tgcaatttta
     3841 cgtactctgc tcattcaaat ccctggctta attttaatac attctgttcc ttgctaaaaa
     3901 tattcagaag tctagagtat ggtgaattgt acaacatttt atccttttat tcccttcatc
     3961 cccattagct ttaggaatat ttaacttaac tagtttaagt aaatccccag ctcagtgaaa
     4021 agaaatataa ttattgattt ataggcaatt tctttattaa aataaccaaa cttaatatag
     4081 gctctatggt tcctaagcac cttcaaccta t
//
LOCUS       DAOQVW010000072         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_24470_length_4101_cov_3.120860, whole genome shotgun sequence.
ACCESSION   DAOQVW010000072 DAOQVW010000000
VERSION     DAOQVW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24470_length_4101_cov_3.120860"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKK85_02435"
     CDS             <1..390
                     /locus_tag="PKK85_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNS24911.1"
                     /translation="LKDQISLIAGGGLRSSADFAKCLALGADAIYLGTAALIAINCEQ
                     YRICHSGLCPTGVTTQNPQLMKQLDLDEGVKKLKNFLTISNQEINNLVRVVGKNNVSN
                     LDHTDLVCTTYDLARLTGLKWLNGKHP"
     gene            429..1448
                     /locus_tag="PKK85_02440"
     CDS             429..1448
                     /locus_tag="PKK85_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNS24912.1"
                     /translation="MKLENLKSGDLFEIVFNSIPDAIAIINREHKVVKVNKSMANSMG
                     MKPEDLMGKNCYEVVHRTSYPINTCPHAMLLHDDQEHYSEIHEENLGGFFLITTTPLY
                     DDDGNVMGSVHVARDITERKIIEEQLEQALEEKDLLMKEIHHRVKNNLMIMASLLNMQ
                     ARYIEDEVARDIFKDSQSRAKTMALIHEKLYQSAELKKIDVQDYMESLARDIYHAYIK
                     DPQKIQLNVKVEQHMLDINTAIPLGLILNEILTNSFKYAFPGDSNGIVNVKFSEKDNK
                     EFLLEVSDDGIGLPLDLHEKKGGSLGMILIHSLTEQIKGEIEINSDHGTKICVKFQDL
                     KYPPK"
     gene            1464..2378
                     /locus_tag="PKK85_02445"
     CDS             1464..2378
                     /locus_tag="PKK85_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HNS24913.1"
                     /translation="MKVLCTTETSLNRPEAVRWRNRMSHLDPVGDVVVVLPCSMKKPY
                     SHSRSHRIFQNVTKGFQEAILTSPFGVCPREMEKTYPIQSYDVPTTGNWSQEEISLTG
                     ECLKTYIGEIEVVAHVSGGYQDVCEKYIPQAHFTCEDGRTTSQESMQNLKNILKSYSK
                     PKGKAREINGLKSIVRYQFNSKDADVLIPSGYRLKGRYNKHLFFENQLVATLQFDTGL
                     FSLNLKGGQLLSEVDLNWVEIDFKLETNTLFAPGVIDSDSKILPRDEVVIIRKGDVIG
                     VGKAVMSGEEMKKAKKGVAVRVRHRQKH"
     gene            2533..2823
                     /locus_tag="PKK85_02450"
     CDS             2533..2823
                     /locus_tag="PKK85_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNS24914.1"
                     /translation="MSEDLMNQVKEALSVVLDPHMRISIVEMGIVDDIEINEKNKTAK
                     IVIKPTNPGCMSAASMAMQARQAAEGVEGIDKVEIVVEGHMMADAISEMVNK"
     gene            2824..3366
                     /locus_tag="PKK85_02455"
     CDS             2824..3366
                     /locus_tag="PKK85_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS24915.1"
                     /translation="MLLWNRVAVVGVPGVGKTTLCRAINKKLGYHYINYGELMLEIAI
                     EKDLAHTMEFMLQLKPELQFQIWESAAFSILNVKNLLIDLHGVDLTREGYLISLPFEI
                     IPPEIIIIIEASYDEIFQRRCKDVVKNRVLEDYKSVKEHMSLLKYSMSSISTTLGSNL
                     MILKNDDFQTCLKHLEAVLR"
     gene            3534..3606
                     /locus_tag="PKK85_02460"
     tRNA            3534..3606
                     /locus_tag="PKK85_02460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3567..3569,aa:Ile,seq:gat)
BASE COUNT         1378 a          722 c          815 g         1186 t
ORIGIN      
        1 ctaaaagacc aaatctcttt aatagccggg ggtgggttgc gtagttcagc agattttgcc
       61 aaatgtctgg cactaggtgc tgacgccata tatctgggta ccgctgctct catcgccatt
      121 aactgtgagc aatatcgtat ctgtcacagt ggcctatgtc ctaccggagt gactacccag
      181 aaccctcaac tcatgaaaca gctggatctg gatgaaggtg tcaaaaagct aaaaaacttc
      241 cttacaatat ccaatcagga aataaacaac ttagtacgtg tggtgggtaa aaataatgtt
      301 tctaacttgg atcacaccga tctggtctgt accacctatg acttggcccg acttactggg
      361 cttaaatggt tgaatggaaa acatccatga cgctataaaa aaactaatat gaataggagg
      421 gagttaaaat aaaattagaa aatttaaaat caggagattt atttgaaata gttttcaact
      481 ccattccaga tgcaatagcc attattaacc gtgaacataa ggttgtgaag gttaataaga
      541 gtatggcaaa ttctatgggt atgaaacctg aagatttgat gggtaaaaat tgttacgaag
      601 ttgtccaccg cacatcatac cccattaaca cttgtccaca tgctatgctc ttacatgatg
      661 accaagaaca ctattcagag atacatgaag aaaatttagg cggattcttc cttattacca
      721 ccacacctct ctatgatgat gatggtaacg tgatgggaag tgtacatgtg gccagggaca
      781 ttacagaacg gaagataata gaggaacaat tagaacaggc cctggaagaa aaagatttgc
      841 tgatgaaaga gattcatcat cgggtgaaga ataacctcat gatcatggcc agcttgctga
      901 atatgcaggc ccgatacata gaagatgaag tagccaggga tatttttaag gacagtcaga
      961 gtcgcgccaa gaccatggct cttatacacg aaaagttgta tcaatctgca gaacttaaga
     1021 aaatagatgt acaagattat atggagagct tggctaggga tatttatcat gcctacatca
     1081 aggatcctca gaaaatccag ctaaacgtaa aagtggaaca gcacatgctg gatatcaata
     1141 ctgccattcc cttgggattg attctcaacg aaattctgac caacagtttc aaatacgcct
     1201 ttccaggaga ttcaaatggc attgtcaacg ttaaatttag tgaaaaagat aacaaagaat
     1261 ttttattaga agtatctgat gatgggatag gtttgcctct ggacctccat gagaaaaaag
     1321 gtggatctct cggtatgatt ctaatacata gtctcaccga acaaattaag ggtgaaatag
     1381 aaataaattc agaccacggc accaagatat gtgttaaatt tcaggatctg aaatacccac
     1441 caaaataaga aatgaatcat atgatcaaag tactttgcac caccgaaact tctcttaatc
     1501 gtcccgaagc agttcgatgg aggaatagga tgtcccattt ggatcccgta ggtgatgtgg
     1561 tggttgtttt accttgcagc atgaaaaaac cttactccca ctctcgttca caccgtatct
     1621 tccagaatgt aactaaagga ttccaagaag ccattttaac ctcacctttc ggagtatgtc
     1681 cccgagagat ggagaaaaca tatcccatcc aatcttatga tgtccccacc accggaaatt
     1741 ggtcacaaga agagatcagc ctaactggag aatgtttgaa aacttacata ggggaaatag
     1801 aagtggtagc ccacgtatca ggaggttatc aagatgtttg tgaaaaatat attccccaag
     1861 ctcattttac ctgtgaagac ggccgcacca cctcccaaga gtcaatgcag aatttgaaaa
     1921 atattttgaa gagttatagt aaacctaaag gtaaagctag agaaataaat ggtttaaaat
     1981 ccatagtacg atatcagttt aatagtaaag atgcagatgt gctcatacct tccggttatc
     2041 gtcttaaagg tcgatataat aaacatttgt tctttgaaaa tcaattggta gccactttac
     2101 aatttgatac tggcctgttt tctcttaacc ttaagggagg tcagctttta agtgaagttg
     2161 atttgaactg ggtggaaatc gattttaaac tggaaactaa cacccttttt gccccaggag
     2221 ttattgattc cgactcaaaa atattacccc gagatgaagt ggtaataatt cgtaaaggag
     2281 atgtgatagg ggttgggaaa gcagttatga gtggggaaga aatgaaaaaa gccaaaaaag
     2341 gagtggcggt acgggtcaga caccgccaga agcactgaaa atctttttca tccttcacaa
     2401 actttaaata ccagaactaa aacattagaa aataacttta taggtggata taaagttata
     2461 aaagttcatt tatgagataa aaacctattt aaagacttaa aatatgttgt attatttttt
     2521 tgaggtataa aaatgagcga agatttaatg aaccaggtta aagaagcctt atcagtagta
     2581 ctagaccccc acatgcgcat tagcattgtg gaaatgggaa ttgtagatga catagaaatc
     2641 aatgaaaaaa ataaaaccgc aaaaatagtg ataaaaccaa ctaaccccgg ttgcatgagt
     2701 gccgctagca tggccatgca agcccggcaa gctgccgaag gcgtggaagg cattgacaaa
     2761 gttgaaatag ttgtggaagg acacatgatg gctgatgcca tcagtgagat ggtaaataag
     2821 taaatgctcc tctggaatcg agttgcagtg gttggtgtgc ctggagtagg gaagaccact
     2881 ctatgtagag ccatcaacaa aaaactcggt tatcactata ttaactacgg ggagttgatg
     2941 cttgaaatag ccattgaaaa ggatttagct cacaccatgg agtttatgct ccagcttaaa
     3001 ccagaattac agttccaaat atgggaatct gcagctttta gcatactgaa cgtgaaaaat
     3061 cttctcattg atttacatgg tgtggaccta acaagagagg gttatttaat atcactgccc
     3121 tttgaaatta ttcctcctga gattattatc atcatagaag catcctatga tgagattttt
     3181 caaaggcgat gcaaagatgt agtaaaaaat agagttttag aggattataa aagtgtaaaa
     3241 gagcatatgt cccttttaaa atattcaatg tcctcaataa gcactacttt aggttctaat
     3301 cttatgattc tcaaaaatga cgattttcaa acttgtttaa aacacttaga agctgtttta
     3361 agataatgac tgtgactggt ggctgtttaa gatgtggtgc cctattattt caccgggact
     3421 gaatttagaa aaggatatat atgatgaagt ttaaaccttg catctaccta ctctactttt
     3481 agggttgtta atgagctatc taagtttttt tagataactt gatattttat ggcgggcccg
     3541 tggctcaggc ggtagagcgc acggctgata accgtgaggt cctgggttcg aatcccagcg
     3601 ggcccattat attctgtata caattgtaat tttttttaac caggttaact atgctgagaa
     3661 ttatattttg accttaagag tatacccgta aaatagattg atcatggcga ataattttaa
     3721 aatgttatta aattaaatta ataatataat aattcaaaaa ccttatattc caccagtacc
     3781 tatcatagaa ttggagcaat tggtaacttc ggtatattat tcacctattt tgttaccaat
     3841 ttgccatttt ttttgtttta attagacttt taaatacatt attagagtta ataatcaatt
     3901 ttagtatttt aattattgat ttaaaccaat tatacctgta tagaacgccg acatatttat
     3961 tataatcttt tctttttaca atcgacggtt tgagaggggt tagtctacac agcatggctc
     4021 tatctatgaa aaaagtttcc acctaatttt ctcgattttg ataaacatct cagattttga
     4081 tccacatatc cagatcatcc a
//
LOCUS       DAOQVW010000073         4081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_24625_length_4081_cov_3.458271, whole genome shotgun sequence.
ACCESSION   DAOQVW010000073 DAOQVW010000000
VERSION     DAOQVW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24625_length_4081_cov_3.458271"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..994)
                     /locus_tag="PKK85_02465"
     CDS             complement(170..994)
                     /locus_tag="PKK85_02465"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNS24916.1"
                     /translation="MNTEIYYFSGTGNSLHAARELRKRIPETEQIPIVSLLDRNIIKT
                     NADTVGFIFPIQLAMAPAPVIEFVKKMDLKSAEYIFAVATRAGSQHRAFIDLENILKK
                     KGKTLDSFFSLNMPSNDPKFEGWKPATQEEIRKLESDIQNKLDSIQEIILKKEKIHEE
                     DTEFITPMPAFPILSIFLPFLSRFYNVEFYADSKCIRCGTCEKVCLSGKVKMIDEKPV
                     WQKDVQCFFCHACLNYCPQHAVQIKSTRLLKSFTEENERYSHPYATIDDIAGEKEL"
     gene            complement(1077..1451)
                     /locus_tag="PKK85_02470"
     CDS             complement(1077..1451)
                     /locus_tag="PKK85_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24917.1"
                     /translation="MNKTRIAASILGVFAGVGGGVFHGIGEILQGNVAPKGIYIQAYP
                     AMQATAGEPAITLVPNFLYTGILSIIMGIIVIIWAVAFVGRKNGGLVLILLSVIMLLL
                     GGGIVPAIIGVVGGIIGTRINS"
     gene            complement(1478..2011)
                     /locus_tag="PKK85_02475"
     CDS             complement(1478..2011)
                     /locus_tag="PKK85_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNS24918.1"
                     /translation="MKNKILVAYASKYGSTQEVAEAIVATLREEGFKVDIHPMKKVRN
                     LEEYTMIVLGAPLYLGAWHKDARRFLSRHREALMERQISIFALGPFHAPYDEKEWQAS
                     QGMLEKDLAEFPGLAPVAVEIFGGKFEPAKLGFTDKLIVSMPASPLHKMPASDLRDWT
                     AIRAWANKLAAQLQPDR"
     gene            complement(2050..2868)
                     /locus_tag="PKK85_02480"
     CDS             complement(2050..2868)
                     /locus_tag="PKK85_02480"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNS24919.1"
                     /translation="MGTEIYYFSGSGNSLVVARDVSTKLNATLTPIMSVINQESIENE
                     ANIIGMVFPIYDFRPPEFMARFISKIKNIQSKYIFALCTYGITPGQSLKHLEKTIKSF
                     GGHLSLGYAVEMPQNGLGSGKITKSQQEKMISKWKTRVEEVCDHIQKKKTEEIETSIL
                     FFNILQTQKIRLIPVFFIFLYQVMFKGLDSLAFTSDEECTGCGTCQQICSQNNIELVN
                     HKPSWSDRCINCFACLNWCPNKAIHPGDADLSIKNYHHPEVKISDMLKEKKINT"
     gene            complement(2890..3567)
                     /locus_tag="PKK85_02485"
     CDS             complement(2890..3567)
                     /locus_tag="PKK85_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNS24920.1"
                     /translation="MNQEDAASNLHDEIKISTKEKIFNASVDLFSLRGFSDVPVREIA
                     KQAGIREGSIYNHYKNKDAILDAIIDYFKKELGQANLPDEETVSLMKEGPEVFFTRGA
                     QIFLARINTPQFGKIWRLVLMESYRNDKVREFFKKDVLEEPLAGWEEVFKLMIEKKMI
                     KSVNPRTLAYEYWSFVIFLIFDYSILNYEEDFESYLERGIDKMNHHTQLLLEAVKIKD
                     KQSTLKF"
BASE COUNT         1123 a          847 c          769 g         1342 t
ORIGIN      
        1 tcttcgttga cttatcgctt caaagacatc cattttatta acctcctttt attaaaatat
       61 ttctaaattc agcgtattgt aaataataaa aaataattat ataattatat aaatccattt
      121 ccagccagaa tcagcagctt cgatattgga tttagtcatc taagtcttct cataactcct
      181 tttctccggc aatatcatct attgttgcat agggatgaga atacctttca ttttcttcag
      241 taaatgattt taaaagtctt gtggacttga tttgaacagc atgttggggg cagtaattta
      301 gacaggcgtg gcagaaaaaa cactggacat ccttctgcca aaccggtttt tcatcgatca
      361 ttttcacttt tccggataaa cagacctttt cacatgtccc gcatcttatg cattttgaat
      421 cagcgtaaaa ttcaacgtta taaaatctac taagaaatgg aaggaaaatg gaaagaattg
      481 gaaaagcagg catgggagtg atgaattccg tatcctcttc atggattttt tctttcttta
      541 aaattatttc ctggatcgaa tcaagtttat tttggatatc agattccagt tttcttattt
      601 cttcttgtgt tgctggtttc cagccctcaa atttaggatc attactgggc atgttcagtg
      661 aaaaaaagga atccaaagtt ttacctttct tttttaaaat attttccagg tcaataaatg
      721 ccctgtgctg gcttccagcc ctcgttgcca ctgcaaaaat atattctgct gatttcaagt
      781 ccattttctt aacaaattct attactgggg caggagccat ggccagttgg atgggaaata
      841 taaaaccaac ggtatctgca tttgttttta tgatatttct gtctaaaaga cttacaatgg
      901 gtatttgttc tgtttctgga attcgttttc ttaattctct ggcagcatgg agtgaattcc
      961 cggttcctga aaaataatag atttccgtat tcatgattaa atttccagta agttatatct
     1021 tccatccctt atttgaaacc tagtgatcag taggggattg aagttacatt agatctttag
     1081 ctgttaattc gcgtgccgat aattccacct accactccaa ttatggccgg gacgatacct
     1141 ccacccaaga gtaacataat taccgaaagc aggatcagga ccaaaccccc atttttcctc
     1201 cccacgaacg ccaccgccca tattatgact atgatgccca tgatgataga tagtattcca
     1261 gtgtatagga agttgggaac cagagttatg gctggttcac ctgctgtggc ctgcatggca
     1321 ggataagctt gaatgtatat tcctttgggg gctacatttc cctgaaggat ttctccaata
     1381 ccatgaaaca ctccgcctcc cactccggca aatacaccaa gaattgaggc tgcaattcta
     1441 gttttattca tgatgaatct ccatataaaa ttccttatta cctatctggt tggagctgag
     1501 ctgccaattt attcgcccag gctcgaattg ctgtccaatc tcgtaaatcg cttgctggca
     1561 tcttgtgcag ggggcttgcc ggcatactga caattaattt gtcggtgaaa cctaacttgg
     1621 ctggttcgaa cttgccgcca aatatttcaa ccgcaactgg tgctagccca ggaaattccg
     1681 cgaggtcttt ctcaagcatc ccctgtgaag cctgccactc cttctcatca taaggagcat
     1741 gaaagggacc gagggcgaaa attgatattt gtcgctccat gagggcttct ctgtgtcgtg
     1801 acagaaatct acgagcgtcc ttgtgccaag cgcccaagta gagtggtgcc cccaatacaa
     1861 tcattgtgta ctcttcaagg tttcgcactt ttttcatggg atgaatatct accttaaacc
     1921 cctcttcacg caacgttgcc acaattgctt cggccacttc ctgggttgag ccatacttgg
     1981 aagcataagc caccagaatt ttgtttttca tttgatccct ccccttatag tgttattaat
     2041 tatagttaat taggtattaa tttttttttc tttcagcata tctgaaatct tcacctctgg
     2101 atgatgataa ttcttaatac tcagatcagc atctccgggg tggatagctt tattagggca
     2161 ccagttgagg caggcgaaac agtttatgca gcgatcagac caggatggct tatggttgac
     2221 tagttcgatg ttgttctggg agcagatttg ttgacaagtc ccgcaccccg tgcactcttc
     2281 atcagaggtg aaggctagtg aatctaggcc tttaaacatt acctggtata agaaaatgaa
     2341 gaaaactgga attaatctta ttttctgagt ttgtaaaatg ttgaaaaaca ggatactggt
     2401 ctctatttcc tcggttttct ttttttgtat atgatcacat acctcctcca ctctagtttt
     2461 ccatttactg atcatcttct cctgttgact ttttgtaatt tttcctgatc ctaatccatt
     2521 ttgaggcatt tctactgcat atccaaggga taagtggcct ccaaatgatt ttattgtttt
     2581 ttcaagatgt tttagagact ggcctggtgt tattccatag gtgcatagag cgaagatgta
     2641 cttggactgg atgtttttaa ttttagagat gaatctcgcc atgaattccg gtggcctgaa
     2701 gtcatatatg gggaacacca tcccgatgat atttgcttca ttctctatac tttcctggtt
     2761 gatgaccgac ataatggggg tcagagtggc attcaatttg gtggatacat ctctggctac
     2821 caccagtgaa ttgccggagc ccgaaaaata ataaatctct gttcccatat ttctcacccc
     2881 ccatatccat taaaatttaa gcgtgctctg cttatccttg atctttacag cctctaacag
     2941 gagttgggtg tgatggttca ttttgtctat acccctctct aggtaggatt caaagtcttc
     3001 ttcatagttt agaatcgagt aatcaaaaat tagaaaaata acaaaggacc agtattcata
     3061 ggccagggtt cggggattta cggatttaat catcttcttt tcaatcatga gtttaaaaac
     3121 ctcttcccac cccgccagtg gttcttctaa cacatctttt ttaaaaaatt cccttacttt
     3181 gtcattgcga tagctttcca tcaaaaccag tctccatatt ttcccaaatt ggggagtgtt
     3241 gatgcgagcc aggaatatct gggcacccct ggtaaagaaa acttctggtc cctccttcat
     3301 taaagatacg gtttcttcat caggtaggtt ggcctgccct agttctttct tgaagtagtc
     3361 tataatggca tctaaaatgg catccttatt tttataatga ttgtatatgg aaccttccct
     3421 tattccggcc tgttttgcta tttctcttac tggcacatca ctgaaacccc ttagagaaaa
     3481 taaatcaact gatgcattga atattttctc tttagtggat attttaatct catcatgaag
     3541 atttgaagct gcatcttcct ggttcatatt gaaactccag aatttttgaa cgaacatttg
     3601 ttcatttcaa tgttttttat gaaactcccc ctataaaaaa ctaacgtttg ttcataactt
     3661 cctggtaagt ttagactaaa aactgttccc tatgaggaga tctgataaaa aaatttcaag
     3721 cgccccaatt taccaccgga caatgatcat aatttgaggt tgaactcctg gagtaccagt
     3781 aagagaggtg ttaagttttt gggtaaaaat ttagataggt tcccggttta gggttaatat
     3841 gatgggaggt gagagaaaac tataagttta tggttctgtt aattaattat taaaattaac
     3901 tttatccctt ttccacttga aaagcaaggg gtgggtacta tagccaagtc cagggaagca
     3961 atagagaaac aatttgacaa gttaagacag aacctcctag atctgaccat gcgtaatcag
     4021 cttctgaatt tcaggccccg aaccagggct atacccatgg aagatgaaaa cccggaagaa
     4081 c
//
LOCUS       DAOQVW010000074         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_24670_length_4077_cov_2.689955, whole genome shotgun sequence.
ACCESSION   DAOQVW010000074 DAOQVW010000000
VERSION     DAOQVW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24670_length_4077_cov_2.689955"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..650
                     /locus_tag="PKK85_02490"
     CDS             189..650
                     /locus_tag="PKK85_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS24921.1"
                     /translation="MAPSFPWIFLIGLYLKKNGDGNLCEKPDKLKDLVLGYRKYTEKT
                     QKELAEELGVPRDIETALESGSYKHPTPRLMDGICELTGAFDQDVLVQIGRGYRLEDE
                     LGPDMKYFIRGLEKVRGIDSTELQALPASKYYKILGSVNMDEYEVIQAGHL"
     gene            complement(780..1472)
                     /locus_tag="PKK85_02495"
     CDS             complement(780..1472)
                     /locus_tag="PKK85_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS24922.1"
                     /translation="MMNSPDKIWFLGRSYQEYLSMFNLDHDFLKDRRVLDCAAGASSF
                     TAWACDNGINATATDVLYGMDLEQLRETFIHDFSTLLQVHSGLDQKVDWGFFQNSEEM
                     IEDRLKTCNNFITHYEKHPERYVQGQLPHLPFTDGHFDLALSSHLLFLYDDRLDYLFH
                     TESIQEMLRVAEEVRIYPIVSLSGYGNRSPYVKRIMEDIGRLGDVDIVKVDYRFRKGG
                     EEMMRLKKDQGS"
     gene            complement(1469..1981)
                     /locus_tag="PKK85_02500"
     CDS             complement(1469..1981)
                     /locus_tag="PKK85_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNS24923.1"
                     /translation="MSYFKIKELLKEHVHSYQVKKFLFNMIQDEFGYGHIPEYHQDIV
                     DLESYYLEPQRNNFFLAVHHTPGIVMGTMGIRAYDKDFPQFKERYQPETTASLWRVFV
                     DRPWRRNGVASALVQMAEDFCRENHYEKIYLHTHKNVQGSLDFWLKNGYQVVADTGNH
                     LKTVHMEKIL"
     gene            2131..2934
                     /locus_tag="PKK85_02505"
     CDS             2131..2934
                     /locus_tag="PKK85_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030410 - nicotianamine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0030418 - nicotianamine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotianamine synthase family protein"
                     /protein_id="HNS24924.1"
                     /translation="MSCYQYWDKIKAIAKSLKQYQDTRFQDLPPQEIIPLLDQVEEIA
                     HDRVIDFDSARHILDDEKMNQALQIIRRFYVEMGSQLETAKAQEILESPDPWATLESF
                     HFYPRYQVLVENENKLVKFSPEQKVAFIGGGPLPLTVILLNKLYGARCVSIEVLPTVA
                     QLSRQVIQKLGMESQIQVLEGDENAVKEVDYTVVMVAALAEPKERVFSNLWEALDLNT
                     PVIYRTYTGMRAILYSPVTEKATRGFHKEVMILPSGKVNNTSVLIRKVI"
     gene            2936..3934
                     /locus_tag="PKK85_02510"
     CDS             2936..3934
                     /locus_tag="PKK85_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS24925.1"
                     /translation="MLMNHHYLKELKNCQLCPWKCGVNRLEGEKGACLMGLPEVAYTG
                     LTPVLQSYSITLLGCSFRCLYCNAYRISQYPDSGWRYRGYVKPEVMVREAVGEFKSSL
                     GRDLGVKRISFTGGEPSLHTPYLEEVSRLIQLQQPGLEVGVATNGFCTTSTMKRLAQL
                     SNYINFEIKAWDTELHQAITGAPVEPVLRNAKWIAQEHPEKIRVFRTVVIPGINQAEV
                     IKIARFIAELDPTLPYRLVGFRPHFMLYYHPAPTRAYLQNLVEECREAGLEQVDFSGY
                     YPGSMMSFSGHNGLKTTYQHLNQAGCYRKPRNCGTCPENHHCPALVLEPWTNPPPG"
BASE COUNT         1060 a         1024 c         1000 g          993 t
ORIGIN      
        1 aaggtggggt acaactctct attgtcaggg accatgaccc caccaactgg agggatctgt
       61 tcagatgaac atcccctccc tagtgcaggg catctaaaaa aaaagcagct caaacatcct
      121 cagtctagat gccctggccc ttacatatta ttttatttac cctccttact ggaagtgggg
      181 gcattgccat ggccccctcc ttcccctgga tatttttaat tggattatat ctaaaaaaaa
      241 atggagatgg taacttgtgt gaaaaaccag ataaactgaa ggacctagtt ttagggtacc
      301 gaaagtacac tgaaaagaca caaaaagagt tggccgagga acttggagtg ccccgtgata
      361 ttgaaactgc cctggaatcg ggatcataca aacaccccac cccacgctta atggatggga
      421 tctgtgaact gaccggggca tttgatcagg atgtcctggt acagataggg aggggctatc
      481 gactcgaaga tgagctgggt ccagatatga aatactttat acggggcctg gagaaagtta
      541 ggggaataga ctccacagaa ttacaagcac tcccggctag taagtactac aagatactgg
      601 gcagcgtaaa catggatgag tatgaggtta tccaggccgg gcatctttaa ctacaccccc
      661 cacttttttt aaattttttt ttgttcatct ataatcaggg tgatgcttcc cctctactta
      721 ttttgggagg tcttcctcac tacttcgggt atggtggatg tgatgagaag gattgaattt
      781 tatgatccct ggtccttttt taggcgcatc atctcctccc cacccttgcg gaaccgataa
      841 tccaccttca cgatatccac atcacccagc cgtcctatat cttccattat tcttttaacg
      901 tagggtgatc tattcccata tccacttaaa ctgacaattg gatagatcct gacttcttca
      961 gccaccctta acatctcctg gatggattca gtgtggaata gataatccag gcggtcatca
     1021 tagagaaaca gtagatggga ggagagggcc aagtcgaagt ggccatctgt aaatgggagg
     1081 tggggtaact ggccctggac gtatctttca gggtgctttt cgtagtgggt gatgaagttg
     1141 ttgcaggttt ttaaccggtc ctcaatcatt tcttctgaat tctggaagaa tccccagtcc
     1201 accttctgat ccaggccaga atggacctgg agtagggtgg aaaaatcgtg gatgaatgtt
     1261 tccctgagct gctctagatc catcccgtac aggacatcag tggctgtagc attaattcca
     1321 ttatcacagg cccatgcagt gaaggagctg gccccggctg cgcagtccag tacccgacga
     1381 tctttcagaa aatcatgatc caagttgaac atggacaggt attcctggta actccggccc
     1441 aggaaccaga tcttgtctgg ggagttcatc atagaatttt ttccatgtgc acggttttaa
     1501 gatggttccc ggtgtcagct actacctggt aaccgttttt cagccagaaa tccagggacc
     1561 cctggacgtt cttatgggtg tggagataaa tcttttcgta gtggttctcc cggcagaaat
     1621 cctcggccat ctggaccagg gcagaggcca ctccatttct acgccagggc ctgtccacga
     1681 atactctcca caggctggct gtggtctctg gttggtaacg ttccttaaac tgggggaagt
     1741 ccttatcgta ggcccggata cccatggtgc ccatcacaat tcccggggtg tggtgcaccg
     1801 ccaggaagaa gttgttacgc tggggttcaa ggtagtagga ttccaggtcc acgatgtcct
     1861 ggtggtactc tgggatgtga ccgtagccaa attcatcctg gatcatgtta aaaaggaatt
     1921 ttttaacctg atatgaatgt acatgttctt ttagcaactc ttttattttg aaatagctca
     1981 tactaacttt ccaccccaaa actattgtaa tacttttttc tggttaacca cgaaataagt
     2041 aatacttatg ccatggttta tattaaataa agggtgaaat aagatatata aaatttttca
     2101 tagtaatgta ccaggaaaag gagaatagaa atgagttgct accagtactg ggataaaata
     2161 aaggccatag ccaagtccct gaaacagtat caggacacta ggttccagga cctgcccccc
     2221 caggaaatca tacccctcct ggaccaggtg gaagagatag cccatgaccg ggttattgat
     2281 tttgactcgg ccaggcacat cctagatgat gagaagatga accaggcact ccagatcatc
     2341 cgaagattct acgtggaaat gggctcccag ctggaaactg ccaaagccca ggagatatta
     2401 gagtcacctg acccctgggc tacactggaa tccttccact tctacccccg ctaccaggtc
     2461 ctggtggaaa acgagaacaa gctggtcaaa ttctctccag aacagaaggt ggccttcata
     2521 ggtggcgggc cactccccct tactgttatc ctgttaaaca agctctacgg agccaggtgt
     2581 gtcagcatag aagtattgcc cacggtggcc cagctatccc gccaggtgat acagaagctg
     2641 ggaatggaat cccagatcca ggtgctggag ggagatgaaa acgctgtgaa ggaagtggat
     2701 tacaccgtgg tcatggtggc tgccctggct gaacccaagg aaagagtgtt ctccaacctc
     2761 tgggaggctc tggatttgaa tacccccgta atttaccgta catatacggg tatgagggct
     2821 attctatact cgccagtaac agaaaaggcc acccgtggtt tccacaagga ggttatgatt
     2881 ctgccctcgg gtaaggttaa caacacctcg gtgcttatcc gtaaagtgat ctaagatgct
     2941 catgaatcac cactacctca aggagctgaa aaattgccag ctctgcccct ggaaatgtgg
     3001 ggtgaaccgt ctggagggtg agaagggagc atgcctcatg ggactcccgg aagtggctta
     3061 caccggactc actccagtac tgcagagtta ttcaattacc ctcctgggct gttccttccg
     3121 ctgcctatac tgcaacgcct accgcatatc ccagtacccg gatagtggct ggcgctaccg
     3181 tggctacgtg aaacccgaag tcatggtcag ggaggcagtg ggtgaattta aaagttccct
     3241 gggccgggac ctgggggtga agcggatcag cttcaccggg ggggagccct ccctgcacac
     3301 cccctacttg gaggaggtgt cccgactgat tcagctgcaa cagcccggcc tggaggtggg
     3361 agtggccacc aatggattct gcaccaccag taccatgaaa cgcctggcac agttatccaa
     3421 ctacatcaac ttcgagatca aggcctggga caccgagttg caccaggcca tcaccggggc
     3481 cccggtggag cctgtgctga ggaatgccaa gtggattgca caggaacatc cagagaagat
     3541 ccgggtcttt cgtaccgtgg tcataccagg gataaaccag gccgaggtga ttaaaatcgc
     3601 caggttcata gctgaacttg atcccaccct tccctaccgg ctggtggggt tccgaccaca
     3661 cttcatgctc tactaccacc ccgcccccac ccgtgcctac ctccagaacc tggtggaaga
     3721 gtgccgggag gcgggactgg agcaggttga tttttctggc tactatcccg ggagtatgat
     3781 gtcattttct ggacataacg ggttgaaaac cacctaccag catcttaacc aggccgggtg
     3841 ctaccgtaaa ccacgtaact gtggaacctg ccctgaaaat catcactgcc cagccctggt
     3901 tctggagccc tggaccaacc caccccctgg ttaataccat ccccccttat taatccggat
     3961 tcataatacc aaacccccca tttatattaa taagtagggt gtaaaaagag gaataataag
     4021 agttacactg caacggcagt atccaaacac ccccgaggaa actagaccat gagtatc
//
LOCUS       DAOQVW010000075         4075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_24694_length_4075_cov_3.231343, whole genome shotgun sequence.
ACCESSION   DAOQVW010000075 DAOQVW010000000
VERSION     DAOQVW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24694_length_4075_cov_3.231343"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="PKK85_02515"
     CDS             <1..134
                     /locus_tag="PKK85_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNS24926.1"
                     /translation="IGIAKENGKKTIVIGAEPGFSVALQKSADSAIVLTHGRYPAPH"
     gene            204..392
                     /locus_tag="PKK85_02520"
     CDS             204..392
                     /locus_tag="PKK85_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24927.1"
                     /translation="MNNIDNNTHHLASEFDEEVTVSLPHYHSFYQTVNLIKAMGIDPQ
                     TWLGTGRGTGALVDDATK"
     gene            402..1397
                     /locus_tag="PKK85_02525"
     CDS             402..1397
                     /locus_tag="PKK85_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS24928.1"
                     /translation="MIVVTGATGHIGNVLVKKLLRQGERVRVLVLPQEDLAPIDGLDL
                     EVVEGDVRDLPSLHRAFQGARTVYHLAGIISISPGNEEFIYQVNVVGTRNVVEACIRC
                     GVERLVYTSSVHAFREPPEGKVIDESCTFYTDSVRGAYDRTKAQASMEVIKGLDKGLD
                     AVIVAPSGVLGPCDYRVSQLGHMVISFAKGNLPGYVEGAYDFVDVRDVADGMILSEKK
                     GKSGEVYILAGERVTVEELMIMLEKITGKNPPRLKAPLWLAKAVGKVANVYYRISGAK
                     PLITEYSADVLASNSMISCDKAIRELGYTFRPLEQSLQDSVQWFYENGHLTIQED"
     gene            1493..2503
                     /locus_tag="PKK85_02530"
     CDS             1493..2503
                     /locus_tag="PKK85_02530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNS24929.1"
                     /translation="MPELAPGSVNLVVTSPPYPMIEMWDEMFSLLNYKIADSLKCGNG
                     QEAYILMNQELDKVWVEVDRVLASTGMVCINMGDATRKVGDRFRLYPNHSYLIHSFQS
                     MGYDVLPPVIWRKTSNKPTKFMGSGMMPPNAYVTLEHEYILIFRKDGPRKVSREDMPK
                     RKESSYFWEERNQWFSDLWSDLQGTPQDLPSSHLRNRNAAFPFQLAYRLINMYSIQGD
                     WILDPFLGTGTTTLAAACSARNSVGYELDPHFRKLIGDRIMEVPQFSDKVISQRLASH
                     HAFLKKRSSENKSWKYHSLHYKFGVITRSEESIHFPVARNVEIVDKNQYHVFYEMSDG
                     KS"
     gene            complement(2505..3272)
                     /locus_tag="PKK85_02535"
     CDS             complement(2505..3272)
                     /locus_tag="PKK85_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS24930.1"
                     /translation="MSKTQIPVADYVINPFIGCSHGCVYCYACFMKRFTGHREKWGEF
                     LDVKVNAPELIPTRGSYQGQKILLSSVTDPYLPQEREYKLTRRILENLISLEPSLNIL
                     TKSDLVLRDLDLIKQFPRREIGFSFSTLDKNPITQLERRADSVKKRLYALEKVHQSGI
                     KNYLFISPIFPFITDWKAIIRKSMNFVDYFMFENLNVAGSVWGPVKRWLELEHPEMRD
                     QYHDIYFGNSPYWKEVEQEIMDYCQKQGLEYQIYFHH"
     gene            complement(3563..3823)
                     /locus_tag="PKK85_02540"
     CDS             complement(3563..3823)
                     /locus_tag="PKK85_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24931.1"
                     /translation="MQSLKRFSLKPEDMDMVFINHGHQDHYGNMELFTNAEVLISIHL
                     ARKGSIESKGVKNRSTIADGVHLIYISGYTFEQPPYYCKLKK"
BASE COUNT         1143 a          828 c          918 g         1186 t
ORIGIN      
        1 taattggtat tgccaaggaa aatggtaaga aaaccattgt catcggtgca gaacctggat
       61 ttagcgtggc tctacaaaaa tctgctgaca gtgccatagt cctcacacac ggcaggtatc
      121 ctgctccaca ctagtaatat ttaaagagaa tccttctttt acaatgtttt gtctttaaga
      181 tataatttta atccaggatc aagatgaata atatagataa caacactcac catttggcct
      241 ccgagttcga tgaggaagtc acagtaagcc tcccccacta ccactcattc taccaaacag
      301 ttaatctaat aaaggccatg ggcattgatc ctcaaacttg gctgggtact gggcgcggca
      361 ccggggctct ggtggatgat gccacaaaat agggtggaag aatgatcgta gtaactggag
      421 caactggaca tattggaaac gtgctggtta agaaactgct gagacagggt gaaagagtcc
      481 gagttttagt tcttccccag gaagatttag ctccaattga tggtttggat ctggaagtag
      541 tggagggtga tgtgcgtgat cttccctcac tccatcgggc atttcagggg gctcggacag
      601 tctatcatct ggccgggata atatccattt ctccgggtaa tgaggagttc atctatcagg
      661 tcaatgtagt tggtacccgt aacgtggtgg aagcttgcat ccggtgtgga gtggaacgat
      721 tggtctacac cagttcagtg cacgctttca gggagccgcc tgaaggaaaa gttattgacg
      781 agtcctgcac cttctacacg gactctgtga ggggagctta tgatcgtacc aaagcccagg
      841 cctctatgga agttattaaa ggcttggaca agggcttaga tgcggtgatt gttgctccta
      901 gtggagtttt aggtccctgt gattacaggg tttcccaact ggggcacatg gtcattagct
      961 ttgccaaagg aaatctacca gggtatgtgg aaggtgctta cgactttgtg gatgtccgtg
     1021 atgtggcaga tgggatgata ttatcagaaa aaaagggtaa atccggtgaa gtatacatac
     1081 tcgctgggga gagggtgact gtagaagaat taatgattat gctggagaaa ataaccggta
     1141 agaatcctcc aagattaaaa gcaccattat ggctagctaa agctgtgggt aaggtggcca
     1201 atgtatatta tcgtattagc ggggctaaac cacttatcac tgaatactcg gcggatgttc
     1261 tggccagtaa ttctatgata agttgtgata aggcgattag ggagttgggt tatacttttc
     1321 gtcctctgga acaatctcta caagatagtg tgcagtggtt ctatgaaaat ggccacttaa
     1381 caatccaaga agattaaatt atcaggtacc gttcttttta tattgatgta ttccagaaat
     1441 attacatcaa atgaagactg tgcaccatat tttcttcaaa aatgcggaga gtatgccgga
     1501 actagctcca ggcagtgtga acttggtggt gacatctcct ccatatccca tgatcgaaat
     1561 gtgggatgaa atgttctctc tactaaatta taaaatagct gattcattaa agtgcggcaa
     1621 cggtcaggaa gcttatattt taatgaacca agaacttgat aaagtgtggg tggaagtgga
     1681 tcgagttctg gccagcactg ggatggtgtg catcaacatg ggagatgcca cccgaaaagt
     1741 tggggatcgt tttcgcctgt atcccaacca ctcttacctt atccacagtt tccagagcat
     1801 gggatacgac gtactgcccc ctgtaatctg gagaaaaacc tccaataaac ccaccaagtt
     1861 catgggatcc gggatgatgc cccccaatgc ttacgttaca ttagaacatg aatatatact
     1921 cattttccgg aaggatgggc ccagaaaagt ttctagggaa gatatgccaa aaagaaagga
     1981 gagttcctat ttctgggagg aacgcaatca gtggttctca gatctgtgga gtgatctgca
     2041 gggaactcct caagatcttc ccagttccca tttgagaaac agaaatgcag catttccttt
     2101 ccaattggct taccgtctga taaacatgta ttccatccag ggagattgga tactcgaccc
     2161 cttcctggga accggtacta ccaccctggc tgcggcatgt tcagcccgta attcggtggg
     2221 atatgagttg gatccccatt tcaggaaact gataggagat aggattatgg aagttcccca
     2281 gttttcagat aaggtcatta gccaaagatt agcttcccac catgctttcc ttaaaaaacg
     2341 ttccagtgag aataaatcat ggaagtatca ttcattacat tataaatttg gagttatcac
     2401 ccgttctgag gaatccatcc actttccagt ggctagaaat gtggaaatag ttgataaaaa
     2461 ccagtaccac gtgttctatg agatgagtga tggaaaatcc tagatcagtg atggaaataa
     2521 atctggtact caaggccctg tttttggcaa taatccatga tttcttgttc tacttccttc
     2581 cagtagggag aatttccaaa gtaaatatca tggtactggt ctctcatttc gggatgttca
     2641 agttctaacc atcgcttcac gggtccccag acactgcccg ccacgttaag gttctcgaac
     2701 atgaagtaat caacaaaatt catggatttt cttattattg ccttccaatc ggttataaat
     2761 gggaatatgg ggcttatgaa aagataattt ttgattccac tttgatgaac tttttcaagg
     2821 gcatataatc gctttttaac ggaatcagct cttctttcca actgtgtgat aggattttta
     2881 tctaaagtgg agaatgaaaa accaatctcc ctgcggggga actgttttat gaggtccaga
     2941 tcccgcaaca ccagatcgga cttggttaga atattcaaag acggttcaag ggatattaaa
     3001 ttctctagta ttctacgagt aagtttgtat tctctttcct gtggtagata tgggtcggtt
     3061 acagatgaca ggaggatttt ctgcccctgg tagctgcccc gagtagggat cagttctgga
     3121 gcgttgactt taacatccag aaattctccc catttttccc ggtgaccagt gaatcgtttc
     3181 ataaaacaag cgtaacagta gacacagcca tgactacaac caataaacgg atttataacg
     3241 taatctgcta ctggaatctg ggttttggac aatatagaac gagattctat ctcggtgatt
     3301 ttaatgctgg attccccctg tacttggtgt ttttaaaaaa ttgtagaaca tactaccatg
     3361 tccaggaata atgtaatctg cagttttaac taatttatct aaactttcca tggccatctg
     3421 ttgggatatg aagtcatcat tgtaatccca gtgctttttc gaccgaaata tcctgatgac
     3481 accacaacat cccctgctaa ggctatttta gcctctccaa cacccatgac tctaccttta
     3541 ccttccaagt tcacggtata tctcattttt tcagtttgca gtagtaggga ggctgttcaa
     3601 aggtataccc cgaaatataa atcaaatgaa ccccatcagc tatggttgat ctgtttttaa
     3661 cacctttaga ttcaatggag cctttgcggg ctagatgaat gctaatgaga acttcggcat
     3721 tggtaaaaag ctccatgttt ccataatggt cctggtgtcc gtggtttatg aaaaccatat
     3781 ccatatcctc tggtttaaga ctgaaccttt tcagggactg gatggggtta tctcggttct
     3841 tttcaggttc aattcactat cattgatttt atagtcgaaa ccagtgtcaa ccaggatttt
     3901 acagtccgat tctatgtggg taaccgtgga tccgcctcct acaccacaaa gcagtttcag
     3961 ggttatcaag ttgctttggg ggtttcaaag gaatctagaa gcaaactccc agtttaatat
     4021 ccttaacgga tttaattggc attctctact cttaaagtct agtgtccatg ttttt
//
LOCUS       DAOQVW010000076         4037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25029_length_4037_cov_3.362381, whole genome shotgun sequence.
ACCESSION   DAOQVW010000076 DAOQVW010000000
VERSION     DAOQVW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25029_length_4037_cov_3.362381"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..72
                     /locus_tag="PKK85_02545"
     tRNA            1..72
                     /locus_tag="PKK85_02545"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Val,seq:tac)
     gene            160..231
                     /locus_tag="PKK85_02550"
     tRNA            160..231
                     /locus_tag="PKK85_02550"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:192..194,aa:Val,seq:cac)
     gene            complement(340..1134)
                     /locus_tag="PKK85_02555"
     CDS             complement(340..1134)
                     /locus_tag="PKK85_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNS24932.1"
                     /translation="MDKNRLVRFMARVMEESGFKVYQNFQTSRHIIDIYGVLPTILGD
                     IGVVVACKNYDERWEVGMDVLKEMEMVAKTLKASKVVVVTTSYFTDSANNYAGRRNIK
                     LIDKDGLMVLAKRFTQHQEEIVEEESVVDYDDVEEEMEYTPSQTSGPSVSLGLLSRGK
                     TSLKKGAKGNRGPSGPLWPRIRPFLKNYIVLILIVLLLSKFITYLVELSSSSTVMSGM
                     STIVSSAVLSYGLVLLLERDLNATLVKGTTVFFAAQLVNVLLILYF"
     gene            1292..2065
                     /gene="surE"
                     /locus_tag="PKK85_02560"
     CDS             1292..2065
                     /gene="surE"
                     /locus_tag="PKK85_02560"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNS24933.1"
                     /translation="MAILITNDDGVNSSGILAAKKSVEGLAEIKVVAPATQQSGIGHA
                     LTLFEPIRVTSVNLSDGDNAFSVSGTPTDAVILGIYQLTEEKPELVISGINIGENLGK
                     SELSTSGTIGAAMEAAVHGIPALSVSLQVTRGDIKFHDGQVDVDFTHAQKMVRRVTKQ
                     ILKKGLPVGVDFLNLNIPSHPENNKIKITKLGERMYRVHITERQDPRGRPYFWIDGDP
                     VETDEEGTDVYSLKMEKTTTITPVSLDCTASMDLMENWI"
     gene            2103..2402
                     /locus_tag="PKK85_02565"
     CDS             2103..2402
                     /locus_tag="PKK85_02565"
                     /inference="COORDINATES: protein motif:HMM:NF021397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 family protein"
                     /protein_id="HNS24934.1"
                     /translation="MEAKDFRQRIIKKGAQVKYLGTRTRGTVQQIKLDGEYFWIKMDT
                     TGLFYRSDYLLLVDEILDPKKSKQKLNVHQKIGRLKRIAPTEISDHGDGPGYGGG"
     gene            complement(2636..3634)
                     /locus_tag="PKK85_02570"
     CDS             complement(2636..3634)
                     /locus_tag="PKK85_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HNS24935.1"
                     /translation="MVFVGMDHGTTGVSFTVLRDEPVHFKIGRVQLSRGEISALDELF
                     KLVNPQEIDLMAITYAMGDGISEITPIEKVVDRGILSIEGAGKVTGGGTFVYDEIEQS
                     QVPTILIPGLHKNSESLDPRFRAAYSHQASAEKISICYNAHLETGFEDFIVADISSNT
                     VSILLEDAEIVGAIDACLGAMGIIHGPLDLEKIRDIDEGQRTANQCFSQAGAVKVAGL
                     DEDVSHAREVLLERYQMGDPRAKLALDTMLMTILMEIWGLNGISTKKLEGLVVTGSVG
                     SLREPYDFYGRLTEGFKVAGACVQLPPTSGSMGSAQIARDVFNGARNILGIKVKQF"
     gene            complement(3674..>4037)
                     /locus_tag="PKK85_02575"
     CDS             complement(3674..>4037)
                     /locus_tag="PKK85_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNS24936.1"
                     /translation="GFQTLVEGGYQPELAYFETCHELKLIVDLIYQKGFAGMWHDVSN
                     TAEFGGLSRRERLITDETRAEMKKVLKEIQTGKFAKEWALENQAGKPQLNRQRDIESQ
                     LQIEQEGRKLRKLCGLEK"
BASE COUNT         1121 a          924 c          845 g         1147 t
ORIGIN      
        1 gggccggtgg tctaggggta tgatacctcg cttacaacga ggtgatcagg agttcgaatc
       61 tcctccggcc cactccacat aattttctct gttttgtaat gattccatga gaagagaagt
      121 ttttaaatcc tcggagaatt aatgataatt aggattcacg ggccggtggt ctaggggtat
      181 gatacctcgc tcacaccgag gtgatcagga gttcgaatct cctccggccc actaaaattt
      241 tttttaccac ttggattcag ttaataaact gagggatact gggggttgat gttttgacgt
      301 gcaacaattc ctgaatctat tagttaggga attatgtact tagaagtaca gaataagcag
      361 cacgttcact agctgagctg caaagaacac ggtggtaccc ttcaccaggg tggcattcag
      421 gtcacgttcc aggagcaata ccaagccata ggaaagcaca gcagaagaaa caatggtgga
      481 catgccactc ataacggtgc tgctggagct taactctacc agataggtta taaatttaga
      541 taacagaagc acaattagga tgagcacgat ataattttta agaaagggtc ttatccttgg
      601 ccataatggt ccggaaggtc ctctattccc tttcgcacct ttttttaggc tggtcttccc
      661 gcgagataat aatcctagtg atacactagg gccgctggtt tgggatggag tgtattccat
      721 ttcttcctcc acatcatcat agtccaccac actctcttct tcgactattt cttcctggtg
      781 ctgggtgaag cgcttggcaa ggaccataag cccatcttta tcaatgagtt taatattgcg
      841 acgtccggcg tagttgttgg cactatctgt gaagtaagaa gtagtgacca ccaccacttt
      901 agatgccttc agggttttag ccaccatctc catttcttta agaacatcca tacccacttc
      961 ccagcgttca tcgtagtttt tacaggctac cactactccg atatcaccca ggatggtggg
     1021 aagaacaccg tagatatcga tgatgtgacg agaggtctga aaattctggt aaactttgaa
     1081 cccagattcc tccattaccc tggccatgaa cctgaccaac ctgtttttat ccacttccct
     1141 caccagctct cttgatcaaa tcattgatac ctatatcatt atttaacagt aatattattt
     1201 cttctctacc tttttatact taaaactgtt tctttcccat cccgtaccta tcacgcaaac
     1261 tatttagagg tgtgggtata aatctagctt catggcgatt ctcatcacta acgacgacgg
     1321 tgttaactca tctggaatct tggctgcaaa aaagtcggtg gagggcctgg ctgaaataaa
     1381 ggtcgttgca ccagccactc aacaaagtgg aattggacat gctttaactc tattcgaacc
     1441 aattagagtc acttctgtaa atttatctga tggagacaat gcattttccg tatctggaac
     1501 tcccaccgac gcggttatac tcggaatata tcagcttact gaagaaaaac cggaactggt
     1561 catatctggt ataaacatcg gagaaaacct gggcaaatca gaattatcta cttctggtac
     1621 tattggagcc gctatggagg ctgctgttca tggaatacct gccctatctg tttccttaca
     1681 agtaaccagg ggggatatta agttccatga tggccaggtt gatgttgatt tcacccatgc
     1741 ccagaagatg gttcggaggg tgaccaagca gatactaaaa aaggggctac cagtgggtgt
     1801 tgatttcctg aaccttaaca ttccatccca tcctgaaaac aacaagatta agatcaccaa
     1861 attaggggaa agaatgtacc gagtacatat cactgagagg caggatccca gaggccgacc
     1921 ttacttctgg attgacggag accctgtgga aactgatgag gaaggaactg atgtgtattc
     1981 cctgaagatg gaaaaaacca ccaccataac tccggtatcc ctggattgca ccgcatcaat
     2041 ggatttgatg gagaattgga tatgaactgt tcctaatacc tgtttactgg aaggttaagg
     2101 taatggaagc taaagatttc cggcaacgaa tcattaaaaa aggagctcag gttaaatatt
     2161 tgggaacacg caccaggggc acagttcagc aaataaaact ggacggtgaa tatttttgga
     2221 taaaaatgga tactactggt cttttttaca ggtcagatta tctgctcctg gttgatgaaa
     2281 ttttagaccc taaaaagagt aaacagaaac tcaatgtcca ccaaaaaatt gggagattga
     2341 agaggatagc acccaccgaa atttctgacc atggggatgg tcctggttat ggagggggct
     2401 aagaccaggg ggttgaaaca gtttggagca tccctgtgat gcccacttcc taagattttt
     2461 cttaggttgg tagtatttaa tttaattcta ccgtagaaca gattggggat gatctgcccc
     2521 actattattt ctactgtaga atttataaag tgcaaaaacc atttgtaaat attaaattcc
     2581 tattaaaacc acataaaata ttcataaaag cctgtttgac caggttaatg taattctaaa
     2641 actgtttaac ctttatacct agaatattcc tggcaccgtt gaatacatca cgggcaatct
     2701 gggcactgcc catggagccc gaggtgggtg gtaattggac gcatgcacct gccactttga
     2761 acccctcagt gagtctaccg tagaaatcat agggttccct cagggatccc accgacccgg
     2821 tcacaaccaa cccctctaat tttttggtgg aaattccatt caacccccat atttccatta
     2881 aaatagtcat taacatggtg tccaatgcta attttgccct gggatccccc atctgatacc
     2941 tttccaggag aacctcccgg gcgtggctta catcttcatc caaacccgcc accttaactg
     3001 ctccggcctg agaaaaacat tgattagctg ttctctgacc ctcatctata tcccgaatct
     3061 tttccaagtc taatgggcca tgtattattc ccatggcccc gagacaagca tctattgctc
     3121 ccacaatttc agcgtcctct aggaggatgc tgactgtgtt ggaacttata tcagccacta
     3181 taaagtcctc aaacccagtc tccaaatggg cgttgtagca gatgctaatc ttttctgcac
     3241 tggcttgatg ggaataagcg gccctgaaac gaggatcaag tgactcgctg tttttgtgca
     3301 agcccggtat caggatggtg ggtacctggg actgctcgat ttcatcataa acaaaggtcc
     3361 ctccaccggt aactttccct gcaccttcta tggaaagtat tccccgatca acaacttttt
     3421 caatgggggt tatttcggaa ataccatctc ccatggcata agttatggcc ataaggtcaa
     3481 tttcttgggg attcaccaat ttgaaaagct catccaaggc agaaatttca ccacgggaaa
     3541 gttgaacccg gcctatttta aaatgaacag gttcatccct caatacggtg aaggaaacac
     3601 cagttgtccc gtgatccatc cccacaaaga ccattgtagt acctcatttt taaaaaaaaa
     3661 tttgcagtga ggattatttt tcaagtccac atagtttcct tagcttacgg ccttcctgct
     3721 caatttgaag ttgggattct atgtcgcgtt gtcggtttaa ttgaggttta ccagcctggt
     3781 tttccagtgc ccattctttg gcaaatttac cagtctgtat ttcctttaag acttttttca
     3841 tttcagcacg agtttcatcg gtaattaacc tctccctgcg tgatagtccc ccgaattcag
     3901 cagtgttact gacatcgtgc cacattccgg caaacccttt ctgatagatc aggtctacaa
     3961 tcagtttaag ttcgtgacaa gtctcaaagt aggccagttc tggttggtaa ccgccttcaa
     4021 caagggtttg aaatcct
//
LOCUS       DAOQVW010000077         4017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25194_length_4017_cov_3.783443, whole genome shotgun sequence.
ACCESSION   DAOQVW010000077 DAOQVW010000000
VERSION     DAOQVW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25194_length_4017_cov_3.783443"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1247
                     /locus_tag="PKK85_02580"
     CDS             114..1247
                     /locus_tag="PKK85_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS24937.1"
                     /translation="MKLWALVKKEARDILGNRIFLLVVVVQLLIILGTFGLALSTALV
                     NDPAVLDSYGVNSAIKVGLSEDLNQSTLVDDLEAQKLQIEYFNTTQEAKRLLGTDLAA
                     VISISSEGEIQVEFDTSNVFYPVASTKVSKAVDTFQLRKNLQSRELSEEQIKVIQEPV
                     KLRIINLKEEEAVKIALDSPYFVEVMYGFIVPFILLLPFFMASNLVTDSIVGEKERKT
                     FEVLLMSPLPSSMVVIGKIIPILTFSLLQSLAWILLLDLLQVPIFNTLLLLLILFSVG
                     LGFIGVGILISVLVDSTKEANSAITLILVFTTFILFVPLFIKSGMFQVLFSFIPTVLM
                     VKIASSPVVNPEVVVYLLPTILISILIFLGTVRSFRHERAIRL"
     gene            complement(1301..1885)
                     /locus_tag="PKK85_02585"
     CDS             complement(1301..1885)
                     /locus_tag="PKK85_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HNS24938.1"
                     /translation="MRNLCPDTMSREHLRQLLHASGVFVVVLSYLIPVKVVIIICLVL
                     LVFLVLIFRWDRYLHIPIFSPILRYCKRSEDEVGSIYFFLGIIITLFFFQSHLDVANA
                     AILILLFGDSASTLVGERWGNIKLPFRKDKTFEGSLAFLAVGFIVASTQLPLIPAISG
                     AFFGALTEAYSPIDDNLTIPLVTGLMMSLVVYFM"
     gene            complement(1866..4010)
                     /gene="purL"
                     /locus_tag="PKK85_02590"
     CDS             complement(1866..4010)
                     /gene="purL"
                     /locus_tag="PKK85_02590"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNS24939.1"
                     /translation="MEYIKNELGRDPNPRDYRMLDIMFSEHCSYKSSRPVLALFPTKG
                     EKVIIGPGDDAGIVDLTEDLALVMGMESHNHPSAIEPYGGAGTGIGGIIRDIISMGAM
                     PVALLDSLRFGPLEDQRSRYLFEYAVKGISDYGNQVGVPTVGGEVEFHENYRLNPLVN
                     VVCAGLVRKDSIIRGIAPNVGDVFVLMGGRTGRDGIHGVTFASEELTSSSELESRPAV
                     QVADPFTKKQVMEATLEALEEIDIQGLKDLGGGGLTCCISEMAAKGGNGALMELSKVP
                     LREEGMTPYEIMLSESQERMVFVVKPGDVEILLAIFQKYELSYAVIGEVTDDGLMVVT
                     REGEVMAELPCELLADPPLVEREAREPVRDDEYVELVDSSADEALLALLSSLNIASKK
                     WVYRQFDHEVQIRTVVKPGDDAAVLRVDQERGFTLTTDCNNIHCYLDPYHGGAGAVAE
                     AIRNVVAMGSEPLCMVDCLNFGNPEKPEVFWQFKECVQGMSDLAHRFNLPVISGNVSF
                     YNETEGVTVNPSPVVGVAGVMDLKDIRTTPFKNEGDVIILIGHTHPELDGSAYHKTMH
                     GVMQGKAPQVDIEAEYQSAQAVLDLVKNDDQGSITALHDLSNGGLGVALAEMALEGGL
                     GAVVDLSSVPGNLDMTPIQVLFSESHSRYLLTVQEGSAHEVITHLTGNGVPTASIGRV
                     EGKMLVIDQAGLTINVEELKNAYQGAVEKFMS"
BASE COUNT         1081 a         1000 c          867 g         1069 t
ORIGIN      
        1 caaagggata aagtaatttt tgggccaaga ccgagtatta ttaggctaac catggagtta
       61 atgggtctta acaaggacaa aaaaactaga tcccataaaa ggagaatccg caattgaaat
      121 tatgggccct cgtaaaaaaa gaagcccggg atatactggg gaaccggatc tttttactgg
      181 tggttgtggt tcagttactg ataatcctgg gaacattcgg actggcttta tcaactgctc
      241 tggttaatga ccccgcggtt ctagatagtt atggggttaa ctctgccatc aaagtgggat
      301 tatcggagga tttaaatcaa tcaacactcg tagatgattt agaagctcaa aaacttcaaa
      361 tagaatattt caacactacc caggaggcta aaagactttt gggaactgac ttggcggcgg
      421 ttatcagcat atcttctgag ggggagattc aggttgaatt tgacacttcc aatgtttttt
      481 atccagtggc ctctaccaag gtaagtaaag cagttgacac ttttcagctc cggaaaaacc
      541 tccaatcaag agaattaagt gaagaacaaa ttaaagttat ccaggaacct gttaaactgc
      601 gaattatcaa cttaaaagag gaagaagcgg ttaagatcgc cctggacagc ccctactttg
      661 tcgaggtaat gtatggcttt atagtgccct tcatactctt attaccattt ttcatggcta
      721 gtaacttggt aacagatagc atagtggggg agaaggaacg aaaaaccttt gaagtactcc
      781 ttatgtctcc tctgcctagt agtatggtgg ttattggtaa gattatcccc atattgactt
      841 tttccttgtt gcagagtctg gcgtggattt tattgctgga cctgttacag gtgccaatct
      901 tcaacaccct gctcctgctc ttaatacttt tctctgtggg attagggttt attggagtgg
      961 gaatactcat atctgtcctg gtggacagta ctaaggaggc gaactcagcc attaccctga
     1021 tcctggtctt caccactttc atactctttg tccctctatt cattaaatcg ggcatgtttc
     1081 aagtgctgtt tagcttcata cccacagttt taatggttaa aattgcttcc agtcctgtgg
     1141 taaatcctga agtagtggtt tacttactgc ccacaatttt gatatctatt ctgatattcc
     1201 tgggcacggt ccgatccttc cgtcatgaaa gagctattcg tctgtagatg aaaaaaagat
     1261 tcggggaaaa aattaatgaa tgacacaagt ttaccatcat tcacataaaa taaaccacca
     1321 gactcatcat cagtccagtt accaggggta tggtgagatt atcatcaatg ggactgtacg
     1381 cttcggtcaa tgccccaaaa aaggcccctg aaatggcggg tatcagggga agttgggttg
     1441 atgccacgat aaaaccaact gcaaggaagg ctaggctccc ctcaaatgtt ttatccttcc
     1501 gaaatggtaa cttgatgttt ccccatcttt cacccaccag tgtagatgcg gaatcaccga
     1561 aaagcagtat taaaatggcg gcgttggcca catccaagtg ggattgaaag aagaaaaggg
     1621 tgattataat tcctaggaag aaatagatag atcccacttc gtcctcgctt cttttgcagt
     1681 atcgcaaaat aggggagaaa attggtatgt gaaggtaacg atcccagcgg aatatcagca
     1741 ccagaaaaac taaaagaaca agacaaatga taataacaac tttcactggt attaagtagc
     1801 tcaatactac tacgaagacg cctgaggcat gcaacaactg cctcaggtgt tccctgctca
     1861 tagtatcagg acataaattt ctcaacagct ccctgatagg cgtttttaag ctcttccacg
     1921 tttatagtca atcctgcctg gtcaatgacc agcatttttc cctccactcg cccgatggac
     1981 gcggttggca caccgttacc tgtaaggtgg gtgatgactt catgggcaga accttcctgc
     2041 actgttagca agtatcggga gtgggactcc gagaacagaa cctgtatggg agtcatatcc
     2101 aagttaccag ggacactgga aagatccacc accgccccta aacctccctc cagggccatt
     2161 tcagccaggg cgacgcctaa acctccgtta gagaggtcgt gcaaggcggt aatgctcccc
     2221 tgatcatcat ttttcaccaa atctagcacg gcctgggcag attgatattc tgcttcaata
     2281 tctacctggg gtgctttccc ctgcatcacc ccgtgcatgg ttttatgata tgcggaacca
     2341 tccagttcag gatgggtgtg acctattaaa atgatcacgt caccctcgtt cttgaagggt
     2401 gtggtgcgga tgtctttaag atccatgacc ccggccacac caacgactgg tgagggatta
     2461 actgtcaccc cctcggtttc gttgtaaaaa ctaacgtttc cacttataac tggtaaattg
     2521 aatcgatggg ccaaatctga cataccctgc acacactcct tgaactgcca gaatacttct
     2581 ggtttttcag ggtttccaaa gttaaggcaa tccaccatgc aaagtggttc agaccccatg
     2641 gccaccacat ttctgatggc ctcggccaca gcaccagcac caccatgata ggggtccaga
     2701 tagcagtgta tgttgttgca gtctgtggtg agggtgaaac ccctctcctg gtccactcgc
     2761 agcacggcag catcgtctcc gggtttgacc acggtacgga tttggacttc atgatcgaac
     2821 tggcgataca cccacttctt actggcaatg ttcagggatg ataaaagtgc taaaagagct
     2881 tcatcagctg atgaatccac taactcaaca tactcatcat cacgtactgg ttcacgggct
     2941 tctctctcaa ccagcggagg atcagccagt aattcgcagg ggagttccgc cataacttct
     3001 ccttcccgtg taaccaccat aagtccatca tcagtcacct caccaataac agcgtaggac
     3061 aactcatatt tttggaatat ggccagcaaa atttccacat cccccggttt aactacgaaa
     3121 accattcttt cctgggattc ggatagcata atctcgtagg gagtcattcc ctcctccctg
     3181 agaggtacct tgctcaactc catcagcgcg ccattacccc ccttggctgc catttcggat
     3241 atgcaacagg taaggccgcc acctcccaga tctttaagtc cctgtatgtc aatttcctcc
     3301 agagcttcca gtgtagcttc catcacctgt ttcttggtga atggatcagc tacctggaca
     3361 gcaggccgac tttctagctc tgacgatgat gtgagttcct cagaggcaaa ggtcacacca
     3421 tggatcccgt cgcgtccggt gcgtcctccc atcaggacaa agacgtcacc aacattaggg
     3481 gctatccccc ggattatact atctttccgg accaaccccg cgcacaccac atttaccagt
     3541 ggattcagcc gataattttc atggaactcc acttcacccc ctacggtggg tacacccacc
     3601 tggttaccat aatcagaaat tcctttaact gcgtattcaa aaagataacg gctgcgctga
     3661 tcttctaggg gcccgaaacg tagtgaatct agaagtgcaa ctggcatagc ccccattgat
     3721 attatatccc gtataatgcc accaataccc gtaccagccc caccataagg ttctatggct
     3781 gaggggtggt tgtggctctc catgcccatc accagggcca agtcttctgt tagatcaaca
     3841 atacccgcat catcaccagg tcctataatc accttttccc ctttggtggg gaacagggcc
     3901 agcaccggcc ggctactttt atatgagcaa tgttctgaga acatgatatc cagcatacga
     3961 tagtcccggg gattagggtc ccgccccaac tcatttttta tgtactccat ttcctct
//
LOCUS       DAOQVW010000078         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25273_length_4009_cov_3.346737, whole genome shotgun sequence.
ACCESSION   DAOQVW010000078 DAOQVW010000000
VERSION     DAOQVW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25273_length_4009_cov_3.346737"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKK85_02595"
     CDS             complement(<1..639)
                     /locus_tag="PKK85_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNS24940.1"
                     /translation="MKIEMEGPIILRKTTRMRGFIDSVAQVNPKLWRWVMNGGIVVAV
                     FLMAFIVYYLVASLETVAVNPQVSIVLPGVKIPGSPIYLPFGYGLIGLITVIVVHEFG
                     HGILARVEKVNIKSLGVGLLAILPIAFVEPDEEEVNKLGSLSKLRIFAAGSIFNMMLA
                     ALTIAIYLVLTLFLIPYALPAEGLEIVNVVPGSPADGVLQEGMVIQAINGYPV"
     gene            complement(738..1952)
                     /locus_tag="PKK85_02600"
     CDS             complement(738..1952)
                     /locus_tag="PKK85_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNS24941.1"
                     /translation="MFLYELMPVEDAKKLLKSNLRTVGVEMADLEEVHRRVLAQDLFS
                     LLDSPPFDRSAMDGYALQAEETFGHSVDNPVQLDIVDRLGAGNVSNVDLKEGEAIQIA
                     TGAPLPSGANAVVMEEFTQSHQDHLQVETAVIPGENVSPAGEDFKTGDMILRQGSVLN
                     PPEVGIVASAGYREVKVYKKPAVGVMVTGSELVMPKSQLEGAEIINSNHFSVKFMVES
                     ALAVPDMTHCVDDYDLVRREFLRLLKNNDCLITTGGTAISKGDVVVDVAAELGEVLIH
                     GVTLRPGKPFAFAVVEEKPVFMLSGFPVAAMVQFDVFVREILLGMQGFKRELHFITKN
                     STRKIPSTLGRTDYIRARIEGDAVRPLKIKGSGIIKSMVDSDCYIVMDENLEGVGSGE
                     KCQVLPYHSLQV"
     gene            2364..3275
                     /locus_tag="PKK85_02605"
     CDS             2364..3275
                     /locus_tag="PKK85_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS24942.1"
                     /translation="MISMDSLSKSFGNIKALDNLNLDVKKGELLGVIGPNGAGKTTAI
                     RITCCIMKPDQGDVLVDGYSIHKDPLKIKSLIGYLPEEPNLYERFKARDLLKYFSELY
                     GVPGADINQRVEELLELVGMTHRAEDRINTFSKGLRQRISIARALVHDPEIIIFDEPT
                     MGLDPATSQTIRHFINQLKGDKTIILCTHYMDEADQLCDRVALLDQGRIRDMGTPQDL
                     KSKIHGDLILLVRVTNPLESDQEVISGFRSVENLETEDEQYIISLNSREEISQILNVL
                     GSRALAVNTKEPTLEDVFISTLENRRI"
     gene            3275..>4009
                     /locus_tag="PKK85_02610"
     CDS             3275..>4009
                     /locus_tag="PKK85_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS24943.1"
                     /translation="MNFMTIARWEFKNTIKSRKFLVIFFLQIAALFLFVMVFNSLAEN
                     LESNEALSITPSLSGFANVDVDDQGGLFTRHLNSEVLTLKNSSYQQARNRLERGLTVG
                     YYTVPPESVTLIQDGDQVETILYLDYRDPKRSVVREEVNSTTKTLSTALTTSYLESYR
                     PRNITKQPGVTETTSGESVPLQLIRKVMLSVLLFLPLFLFGNLIIDSVVGEKERKTVE
                     MLRALPLTSADIIIGKSLAVVMVMALQ"
BASE COUNT         1149 a          993 c          823 g         1044 t
ORIGIN      
        1 gactgggtag ccattaatgg cctgaatcac cataccctcc tgtaatactc catcagccgg
       61 actaccaggc accacgttca caatttccaa accttccgct ggtaaagcgt agggtattaa
      121 gaacagagtc aaaacaaggt agatggcaat ggtcaaggcc gccagcatca tgttgaagat
      181 ggatcctgca gcgaagatgc gcagcttgga aagactgccc agtttattca cctcttcttc
      241 atctggctcc acaaaggcaa tgggtaaaat ggccaataac cccactccca aggacttgat
      301 atttaccttc tccaccctgg ccaggattcc atggccaaat tcatggacta cgatgacggt
      361 gatcaggcca attaatccgt aaccaaaggg taaataaatg ggagagccgg gtatcttcac
      421 tccaggtagc acgatggata cttgggggtt cacagccacg gtttccaggg atgccaccaa
      481 gtagtacacg atgaaggcca tcaaaaaaac agccaccacg atacccccat tcatgaccca
      541 ccgccagagt ttaggattta cctgggcaac ggagtcaatg aagcccctca tacgggtagt
      601 tttccttaga attatgggtc cttccatttc aatcttcaat ttatccttaa ataagaaagc
      661 taaaacccaa atcacaacaa atgcaatggc ataataccat aacgcgttca aataatcacc
      721 ttttatctta aaacaatcta aacctgcagg gagtggtagg gaagtacctg gcatttttca
      781 cctgagccaa ctccttccag attttcatcc atgacaatgt aacagtcaga gtctaccatg
      841 gactttatga tacccgatcc ttttatcttc aatggccgaa ctgcatcacc ctcgatgcgg
      901 gctctaatat aatcagtacg ccccagtgta gatggaatct tccttgtcga atttttggtg
      961 atgaaatgaa gctctctttt aaaaccctgc attcccagta gaatttccct tacgaacaca
     1021 tcaaattgga ccatagctgc cactggaaag ccggaaagca taaaaacggg tttttcctcc
     1081 accactgcga aggcaaaagg tttacctggt ctgagggtca ctccatggat cagcacttcc
     1141 cctagttcgg cggccacatc caccaccaca tctcctttac tgatggctgt tccaccagtg
     1201 gttattaaac aatcgttatt tttgagtaac cttaaaaatt ctcttcgcac caagtcatag
     1261 tcatcaacac agtgagtcat gtccggaact gccagggcac tttcaaccat gaacttcaca
     1321 gagaaatggt tggaatttat gatctccgct ccttcaagtt gagacttggg catcaccaac
     1381 tcacttccag taaccatcac ccccactgca ggctttttat aaactttcac ttccctatag
     1441 ccagctgagg caacaatacc tacttctggt ggattcagaa cacttccctg cctcaagatc
     1501 atatctcctg tcttaaagtc ttcaccagca ggggatacgt tctctcctgg aataacagcg
     1561 gtttccactt gcagatgatc ttggtggctc tgggtgaact cctccatcac cactgcattg
     1621 gctccggagg gtaaaggggc tcctgttgca atttgtatgg cttctccctc cttcagatct
     1681 acattggaaa cattccccgc tccgaggcgg tccacaatat ccagttggac aggattgtcc
     1741 acggaatgtc caaaggtctc ctccgcctgg agagcatatc cgtccatggc tgacctatca
     1801 aatgggggtg aatccaatag ggaaaacaaa tcctgggcca gaactcggcg gtgaacctct
     1861 tctagatctg ccatttccac gcctacagtt cttaaattac ttttaagtaa ttttttcgca
     1921 tcttccactg gcataagctc gtataaaaac atacaaatct tctctttgat gactgtgatt
     1981 ggaagtttag aatatttagt tttttggcca ccctcccctt tatatgttaa tagggcaagg
     2041 ataggtctac tcattttttt cagacacaac agttaattca ctagacaaca cccaccatat
     2101 aatttaaaaa ttccttaaag aaataaagaa gtttcaacta acactactgg tttaagatga
     2161 gatatcttta atattctcga gagatgtcta tgatgcagta aagaatgacc tatacatgca
     2221 cacccacagt tccaccagac ccatcataac atgatcttga tgttgagtgg gtgtgcttca
     2281 ctttttgata acatctttaa tgttacacaa tcaatatttt gaaacaagaa ctggatgtga
     2341 ataaggtgac ctcttcccaa attatgatca gcatggacag tctttcaaaa tcctttggaa
     2401 acattaaagc cctggataat ctcaacttag atgttaaaaa aggggagcta ttgggggtca
     2461 tcggccccaa cggggcgggt aagacaaccg caatccgcat cacctgctgc atcatgaaac
     2521 ccgaccaggg cgatgtgtta gttgatggat acagcatcca caaagatccc ttgaagatca
     2581 agtccctcat cggttacttg cccgaggaac ctaatcttta cgagagattc aaggcccggg
     2641 acttgttaaa atacttttct gaactttacg gtgttcccgg ggcagatata aatcaacgcg
     2701 ttgaagaact cctggaattg gtgggtatga cccaccgggc ggaggataga attaacacct
     2761 tctccaaggg gctgcgccag cgaattagta tagctcgggc tctagttcac gatcccgaga
     2821 tcataatatt tgatgaacca accatgggac tggaccccgc caccagccag accatccgac
     2881 acttcataaa ccagcttaaa ggtgataaaa ccatcatact atgcacccat tacatggatg
     2941 aagctgacca gctctgtgac cgtgtggcct tactggatca gggacgaata agggacatgg
     3001 gaaccccaca ggacctcaaa tccaaaattc acggtgacct aatcctcctg gtgcgggtga
     3061 caaatccact cgaatctgat caggaagtta tatctggatt cagatcagta gaaaacctgg
     3121 aaacagagga tgaacagtac atcatatctt taaactctcg ggaagagata tcccagatct
     3181 tgaatgtcct gggaagccgg gccctggcag ttaataccaa ggaacccacc ctggaggatg
     3241 tattcataag cactcttgaa aataggagaa tatgatgaac ttcatgacca tcgctcgttg
     3301 ggagtttaaa aacaccataa aaagccgtaa atttcttgta attttttttc tccaaatagc
     3361 agctctgttt ctatttgtca tggttttcaa ttccttagcc gagaacctgg agagtaatga
     3421 ggccctgtct ataacccctt ccctttcagg ctttgccaac gtggatgtgg atgaccaggg
     3481 aggtctgttc acaagacatt tgaattcgga agttctcact cttaaaaatt cctcgtatca
     3541 acaagctcga aaccgtttag aacgcggact aactgttgga tattacaccg taccccctga
     3601 gtcggtgacc ctgatccagg atggagatca agtggaaacc atcctctact tggactaccg
     3661 ggatcctaag cgaagcgtgg tgcgtgaaga agtgaattcc accactaaaa cactatccac
     3721 ggccctcacc acttcttatt tggagtcata caggcccagg aatatcacta agcaacctgg
     3781 agtaactgaa acaacctctg gagaatcagt acccttgcag ttaatacgga aggtgatgtt
     3841 atcggtgctt ctctttttac cactttttct tttcggaaac ctcatcattg acagtgtggt
     3901 gggtgaaaag gaacgtaaaa ccgtggagat gctcagggcc cttccactga cttctgctga
     3961 cattatcatc ggtaaaagtc tagcagtggt gatggtcatg gcactgcag
//
LOCUS       DAOQVW010000079         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25276_length_4009_cov_3.146687, whole genome shotgun sequence.
ACCESSION   DAOQVW010000079 DAOQVW010000000
VERSION     DAOQVW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25276_length_4009_cov_3.146687"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /gene="acs"
                     /locus_tag="PKK85_02615"
     CDS             complement(<1..1861)
                     /gene="acs"
                     /locus_tag="PKK85_02615"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNS24944.1"
                     /translation="MSQEEDTSGRIGGILWPDPQIVKDSNIQVWMNRHQIQSYDQLLE
                     KAVVDPEWFWDEMARELNWQKPYHQVLKWEAPHSQWFQDGNFNIVDNAVDRHARGPHM
                     DKLAFIWQGEDGEVRKMSYHQLYQEVNRFANALQSLGVGKGDTVTIYLPMILELPVAM
                     LACAKIGAVHSVVFSGFWAKRFRERVDEAQSKVAITSDGFSRRGKVLALKANVDRVLL
                     DAPTVEKVIVVRHAKIPVDMTAGRDLWWHELVEGMSEECPTAVLEAEDPLFILYTSGT
                     TGVPKGVLHVHAGYAVGVYTTLKMVFDLKEDDVWWCAADIGWITGHSYIVYAPLLLGV
                     TSVLFEGTPDYPDPGCFWRIVEKYGVNVFYTAPTTIRLFMKYGEKCPQKYDLSSLRLL
                     GSVGEPINPKAWLWYHEHIGGGRCPIMDTWWQTETGMHLITPLPISPLKPGSAFKPFP
                     TIIADVVDDEGNSVVGEGGHLVIKSPWPAMFRTLYRNPLRFRKAYWNTFPGIYLSGDV
                     ARVDEDGYFWVQGREDDVLNVSGHRISTAEVESALVSHPVVAEAAVVGKPHPLKGEEI
                     SAFVILKKDAKEPDNLEMVLKNQVRTEIGPIATPAQVRSVEDLPKTRSGKIMRR"
     gene            2027..3625
                     /locus_tag="PKK85_02620"
     CDS             2027..3625
                     /locus_tag="PKK85_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNS24945.1"
                     /translation="MNNGATTTCAQVMIRTLEEEGVNLVFGHPGEQILPLYQALHHSS
                     IKHVLMRHEQGAAHAADGYARATGGVGVCLASAGPGALNLVMGVATAYKDSVPLLVIT
                     GDLSTQVKGQNTFQDVPQVQVLQPITLQSYDVKKPGEAVSKLKEAFQLFRTGRTGPIH
                     LNIPMDVFSKTVDSSLPGGDLEYISPGYEVDEVKKLLQSSKKPFIIAGAGVIWSGAVE
                     KLRRFAEENMIPVATTYPARGVLPEDHPLSLGLIGLRGTEAANHAGAWADLVLALGCR
                     LSERTLMGLGKTVLIHVNPDPKVLRGEINLQADAGEFLEKISQLRYPDTGEWLNKLQK
                     YRSYHLVDTGYDGLPLKPQRAIKDILGAVRGAVVVNDAGSHTTWVNLLSVVREPSGLV
                     FSGGFGPMGYGLPAAVGVSLARPEKSVVLLVGDGGFQMTLQELAVISQLQLPILIIII
                     NNQGLGIIKQWQELYYDGPYQVALENPDFVSLAASYRIEGQRIKESGQLPYMVQKVLR
                     LRKPYLIEVMVDPDEDIPLPEMKK"
     gene            3622..>4009
                     /locus_tag="PKK85_02625"
     CDS             3622..>4009
                     /locus_tag="PKK85_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515704.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNS24946.1"
                     /translation="MKVLLINPPYTSSKYKFIGLVAPPLGIAYIGAVLEDGGFDVEII
                     DGAALELDWEALEVRVKQAHPQVIAITSLTPTISQGLETAKRARRVCPEATIVLGGYH
                     PSFNHAEILEKSYVDVVVIGEGEYTML"
BASE COUNT          966 a         1065 c         1007 g          971 t
ORIGIN      
        1 cccgacgcat gattttcccg gaacgggtct ttggcaagtc ctccacggat cttacctggg
       61 cgggggtggc gatgggccct atctcggtac gtacctggtt tttcagtacc atttccaggt
      121 tatccggctc ttttgcatct ttttttagta ttacgaaggc ggagatttct tcacccttca
      181 gggggtgggg tttgccaacc accgcggcct cagccactac tggatgactt accagggctg
      241 attcaacctc tgctgtactt atacggtggc ctgaaacatt taaaacatca tcctcccggc
      301 cctggaccca gaagtatccg tcttcatcca cccgggcaac gtctccactg agataaatac
      361 cagggaaggt gttccagtat gcttttcgga atcgcagagg attccggtag agggtgcgaa
      421 acatagcagg ccagggcgac ttgataacca ggtgaccacc ctcacccaca acagaattcc
      481 cctcatcatc caccacatct gcaataatgg tggggaaggg tttgaaggct gaacctggct
      541 tcagggggct gatgggaagg ggtgttataa ggtgcatgcc tgtttcggtc tgccaccagg
      601 tgtccatgat ggggcacctt cccccgccaa tgtgttcatg gtaccacaac caagccttgg
      661 gattaatggg ctcacccaca cttcccagga gccttaagga gctaagatca tatttttgag
      721 ggcatttctc cccatatttc atgaacaggc ggatggtggt gggggcggtg tagaacacgt
      781 tcaccccata cttttccact atcctccaga agcatcctgg atccgggtag tcaggggttc
      841 cctcgaagag caccgatgta actcccaaga ggaggggagc gtacactatg taactgtggc
      901 cggtaatcca tccgatatcc gctgcacacc accacacatc gtcttccttt aaatcgaata
      961 ccatcttcag ggtggtgtac actcctaccg catacccggc gtgcacatgc aacactccct
     1021 tgggcacacc cgtggttccg gaggtgtaca gtatgaaaag gggatcttcg gcttccagaa
     1081 ccgcggtggg acattcttca ctcatccctt ccaccagttc atgccaccac agatcccgcc
     1141 ctgcagtcat atccaccggg atctttgcat gccggaccac aatgaccttc tccacggtgg
     1201 gggcatccag tagcacgcgg tccacattag ccttgagggc taaaacttta cccctcctgc
     1261 tgaaaccatc cgaggtgatg gctaccttgg actgggcttc atcaactctt tcccggaatc
     1321 tttttgccca gaatccagag aaaaccacgg agtgcacggc tccgattttg gcgcaggcca
     1381 gcatggccac cgggagttca agtatcatgg gcaggtagat ggtgacggta tcgcctttac
     1441 ccacccccag actctgcagg gcattggcga atcggttcac ctcctggtaa agttgatggt
     1501 aactcatctt cctaacttct ccatcctctc cctgccagat gaaggccaat ttatccatat
     1561 gtggaccccg ggcatgtcgg tcaacggcgt tgtccacgat gttgaagttt ccatcctgga
     1621 accactgtga atggggggct tcccacttca aaacttggtg gtagggtttc tgccagttca
     1681 gttcccgggc catttcatcc cagaaccatt ccggatccac tacggccttt tccagaagct
     1741 ggtcataact ctggatctgg tgcctgttca tccacacttg gatgttgcta tctttaacta
     1801 tctgggggtc gggccataat attcccccga tcctgcctga agtatcttct tcctgggaca
     1861 tcttacacca tctcaatcct agattgcatt atcttatact atctatgtgc ctatcttcct
     1921 cataattttt ttcaaaaaaa accaccagtt acatatgatg accagtagag ccccccctcc
     1981 tatcctggag aattttaaaa tagagccagc aacataaata cagcacatga ataacggagc
     2041 caccacaact tgtgcccaag tgatgatccg caccttagaa gaagaaggtg tgaacctggt
     2101 cttcggccat ccaggagaac agatcctccc cttataccag gccctgcacc attccagcat
     2161 taaacatgtt cttatgcgcc atgaacaggg agcagcccat gccgctgatg gctatgcacg
     2221 tgccaccgga ggggtggggg tttgtctggc ttcggctgga cccggggcac tgaacctggt
     2281 tatgggggtg gccactgcct ataaggactc cgtacccctc ctggtcatca ccggtgacct
     2341 gtccacccag gtaaagggtc agaacacctt tcaggacgtt ccccaggtcc aggtcctcca
     2401 gcccattact ctgcaaagtt acgatgtgaa aaaaccaggg gaagcagttt ccaagttaaa
     2461 agaagcattc cagctattca ggacaggtag aacaggcccc atacatttga acattcccat
     2521 ggatgttttt tcaaaaacag tggattcatc actcccgggg ggtgacttag aatatatttc
     2581 tcccggttat gaagtggatg aagtcaaaaa gctccttcaa tcatcgaaaa aacccttcat
     2641 catcgccggg gccggggtga tctggtctgg ggcggtggag aaactgcgaa gatttgctga
     2701 ggaaaatatg atcccagtgg ccaccaccta tcctgcccgg ggagttctgc cggaggatca
     2761 tcccctatca ctggggttga tcggattgcg aggaaccgaa gctgctaacc acgctggtgc
     2821 ctgggcagat ttggtgctgg ccttgggctg ccgcctatca gaacgcacac tcatggggct
     2881 gggtaaaaca gtcctgatcc atgttaatcc tgatccaaag gtgttacggg gtgaaattaa
     2941 tctacaggcc gatgctggtg aatttctgga gaaaatctcc cagttaaggt acccggatac
     3001 aggggaatgg ttaaataaac ttcaaaaata cagatcttac catttggtgg acacagggta
     3061 tgatggcctg cccttaaaac ctcaaagagc tataaaggat attttagggg ctgtaagagg
     3121 ggcggtagtg gttaacgatg ctgggagcca cactacctgg gttaatcttc tctctgtagt
     3181 ccgggagcca tctggtctgg tgttctccgg tggcttcggc cccatgggct acggtttacc
     3241 cgctgcagtg ggggttagcc tggctaggcc agagaagagc gtggtgctac tggtgggtga
     3301 tggtggattt cagatgaccc tccaggaact ggcagtcatc agtcaactgc aactacccat
     3361 cctcataata atcatcaaca accagggctt ggggatcatt aaacaatggc aggaactcta
     3421 ctacgatggt ccctaccaag tggccctgga aaacccggat tttgtatccc tggcagccag
     3481 ttatcgcatc gaggggcaga ggattaaaga atcaggccag ttaccctaca tggtacagaa
     3541 agttttaagg ttgaggaaac cttaccttat agaagtcatg gtagacccag atgaggatat
     3601 tcccttacca gagatgaaaa aatgaaagta ctactaataa atcctcccta tacttcttcc
     3661 aaatataaat tcattggcct ggtggctccg ccactgggaa tagcatacat cggggctgta
     3721 ctggaggatg gtggttttga tgtggagatc atagatggtg ctgccctgga actggattgg
     3781 gaagccctag aggtccgtgt aaaacaggcc cacccccaag tcatagccat cacctcccta
     3841 actcccacca taagccaggg actggaaact gctaaacgag ctcgtagagt ctgtcccgag
     3901 gccaccatag tcctgggtgg ctatcatccc tccttcaacc atgctgagat actggagaag
     3961 agctacgtgg atgtagtggt aattggtgag ggggagtaca ccatgctgg
//
LOCUS       DAOQVW010000080         4007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25301_length_4007_cov_3.943320, whole genome shotgun sequence.
ACCESSION   DAOQVW010000080 DAOQVW010000000
VERSION     DAOQVW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25301_length_4007_cov_3.943320"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PKK85_02630"
     CDS             complement(<1..1182)
                     /locus_tag="PKK85_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNS24947.1"
                     /translation="MKSIEEINQKIKDGDAVVVTAADMTRIVRDIGAEKAAKEVDVVT
                     TGTFGAMCSSGAFLNFGHSDPPIKMARTYLNGVEAYSGLAAVDAYIGATQPHRDPKVG
                     LEYGGSHLIEDLVRGEEVELLAEAYGTDCYPLKDIHTHIKLDDLNQAVMVNPRNSYQN
                     YAAATNSSEETLHTYLGTLLPGMGNVTYSSAGELSPLLNDPFFETIGLGSRIFLCGTE
                     GYILGEGTQHSTEVKRINGVPVGSAGTLMLKGDLKKMDVEFLRGATMPFYGPTLYVGV
                     GIPIPVLNPKIAQQTGISDEEIKCLVYDYGVSRRSRPTILETNYHELKSGSVEIDGHE
                     IQTSPMSSHRKASQVAEKLKDWIEGGTFLLTQPVEKVPSKGQTVKPLHIKRPSILVRD
                     LK"
     gene            1418..2791
                     /locus_tag="PKK85_02635"
     CDS             1418..2791
                     /locus_tag="PKK85_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNS24948.1"
                     /translation="MIEELDLNLLERIIHSHEKKADYVDIRVAEAQNTVIVMKDGKVQ
                     EIRSGDDFGASIRVLNNGAWGFSYTTQMSQLESMSEVAFKLSGALESDTELANVNIAV
                     DAVKSRAKIRPQDISIEEKKEVMVEAEKAAQLPKVISTTVNYVEGEGRSYFLSSEGTS
                     LVQEESRVAMFINAVAADAGVIQFGHKSMGGARGFEVIQREDIEKFGRTAASKAVRLL
                     DASSPPSGIFPVLLDPELTGVFIHEALGHASEADLILQKDSILEGKIGTSIGSSQVTI
                     VDDASMDAFGYYAYDAEGVKTRKNVLVKDGVLVSLLSSRETAAKMGLNPSGNSRSAVG
                     DQPIVRMSNTYLKPGDMSFEELVEDMADGIYLKGSRGGQVDTGKGIFQFNAAESFHIE
                     NGEIVDTLRDVSLSGNILEMLTKIDGVGSDFYMGVGFCGKSGQTAPVGDGGPHTRVSE
                     ATVGGAA"
     gene            2791..3351
                     /locus_tag="PKK85_02640"
     CDS             2791..3351
                     /locus_tag="PKK85_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HNS24949.1"
                     /translation="MLTQSEGDFLLKLARTAIESYVLDGKVIITPKSPSPILKENMGV
                     FVTLHINGELRGCIGYPEPVKPLLNALIEVAIGAATGDPRFSPLNSHELEKIHVEVSV
                     LTPPELIVTDRPSEYLEKIDVGHDGLIIEKGLFRGLLLPQVPVEWGWDTEEFLANTCL
                     KAGMTPDCWLQEGVKIYRFQSQIFNE"
     gene            3549..>4007
                     /locus_tag="PKK85_02645"
     CDS             3549..>4007
                     /locus_tag="PKK85_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS24950.1"
                     /translation="MFLPTIPTPDEVVDKAFRRAKKAAMRIRGSKIRGQKKSKRIEEV
                     RVQTACQVIRDTYDDILKKTPRVEELSEFYQDYIDVVVGVDQLKKSLGALKWASDLLE
                     KLQGQYTSKIRRSPPENASKIRREAYGRIVSVVNRIENELDFLTFAKQQLR"
BASE COUNT         1166 a          783 c          926 g         1132 t
ORIGIN      
        1 cttcaaatcc ctaaccagga tggagggacg tttaatatgc agtggtttca cagtctgacc
       61 tttggatgga actttttcca cgggttgggt taataagaat gttccacctt cgatccagtc
      121 cttaagcttc tcggctactt gtgaagcttt tcgatgggag gacatgggag aggtttgaat
      181 ttcatgacca tctatttcta ctgaaccact tttcaactca tgataattgg tttccagaat
      241 tgtaggacgg ctacgtcgag ataccccata gtcgtaaacc aaacacttga tttcctcgtc
      301 acttatccct gtttgttggg ctattttagg gttaagtacg ggtataggta tacccactcc
      361 tacatacaag gtgggcccat aaaaaggcat ggtagcacct cttagaaact caacatccat
      421 ttttttaagg tctcccttga gcatcagggt accggcactt cccactggga ctccatttat
      481 ccttttaact tcggtgctat gctgggtgcc ctcccccagt atatagcctt cagttccgca
      541 gaggaaaata cggcttccca gcccaatggt ctcaaagaat gggtcgttta aaagtggact
      601 aagctctcca gcactggaat aggttacatt acccatccca gggagtaagg ttccaagata
      661 tgtgtgcaag gtttcttcac tggagttggt ggctgcagca taattttggt acgagtttcg
      721 aggattcacc attacagctt gattgagatc atccagtttg atgtgggtgt gaatatcttt
      781 caaagggtaa caatcggttc cataggcttc tgccaaaagt tccacttctt caccccgcac
      841 caaatcctct atcaagtgtg atcctccata ttccagtccc accttgggat cacgatgggg
      901 ctgggtggca ccaatataag catctaccgc tgctaagcca gagtaagctt ccactccgtt
      961 caagtatgtg cgggccattt ttatgggtgg gtcggagtgg ccaaaattta aaaatgcacc
     1021 ggaggaacac atcgcaccaa aggttccagt tgtaacaaca tccacctctt tggcagcctt
     1081 ttccgcaccg atatcccgca ctatacgggt catatcagcg gcagttacca ccactgcgtc
     1141 tccatcctta attttttggt ttatttcctc tatgcttttc ataccgtcac atccggtgcg
     1201 tttaatgaaa aaaattaata attcatcaat tatccatgat ttatgttcat aagaattgca
     1261 gcaattttta ggaatgaaat tgacatgaat atcttcggat ctaggatagt cttgactatt
     1321 taggattact tcttatataa ttactttggc ccaattagga gcggttacct aaattcttta
     1381 ttgatgtggg atcataggaa tagtggtgat aactcacatg atagaagagt tggatttaaa
     1441 tctgttggaa cgaatcatcc attctcatga aaaaaaggca gattatgtgg atattcgagt
     1501 tgcagaggca cagaatactg ttatagttat gaaagatggg aaggtccagg aaatccgttc
     1561 aggagatgac ttcggggctt cgattcgtgt attaaataat ggggcatggg gattttctta
     1621 cacaacccag atgtcacaac tggaatccat gtccgaggta gcatttaaac tttcaggagc
     1681 attagaaagt gacacagaat tggccaacgt gaatattgcg gttgacgcgg ttaaaagtcg
     1741 cgccaagata aggcctcagg atatctccat agaagaaaaa aaggaagtga tggtagaagc
     1801 agaaaaagct gctcaactcc ctaaagtaat aagcaccacg gtaaattatg tggaaggaga
     1861 gggtaggtct tactttttaa gttcagaggg aacatctttg gttcaagagg aaagcagggt
     1921 ggccatgttt attaacgcgg tggcagctga tgcaggagtc atccaatttg ggcataaaag
     1981 tatgggtgga gcccggggat tcgaagtaat tcagagggag gatatagaaa aattcgggag
     2041 aactgctgct tcaaaagctg tcagacttct tgatgccagt tccccacctt ctggaatatt
     2101 tcctgtactc cttgatccag aactaaccgg ggtatttatt cacgaggcct tgggccatgc
     2161 ttcagaagca gacctcattc tgcaaaaaga ttccattctg gaaggaaaaa tagggaccag
     2221 cataggttct tcacaagtaa ctattgttga tgatgccagt atggatgctt ttggatatta
     2281 tgcttatgat gcagagggag ttaagaccag aaaaaacgtt ctggtcaaag acggtgtttt
     2341 ggtgtcactt ttaagctcca gggaaacggc tgctaagatg ggccttaatc ccagtgggaa
     2401 ttcccgttct gcagtaggag atcagcccat agtgcgtatg agtaacacct accttaaacc
     2461 aggagatatg tcatttgaag aacttgttga ggatatggct gatggaatat atcttaaagg
     2521 atcccgaggc ggccaagttg acactggtaa ggggatcttc cagtttaatg ctgcagaatc
     2581 attccatata gaaaacggtg aaatagtcga tactttaagg gatgtttctc tatccggtaa
     2641 catactggaa atgcttacta aaatcgatgg ggtaggctct gatttctata tgggtgttgg
     2701 tttctgcggt aaatcaggac agactgctcc agtgggtgat ggtggacctc atacccgggt
     2761 cagtgaagct acagtggggg gtgccgcgta atgcttaccc aatctgaggg agatttcctg
     2821 cttaaactgg ccagaacagc catagaatcc tatgtgctag acggaaaggt aatcattact
     2881 ccaaaatctc cttcgccaat tcttaaagag aatatgggag ttttcgttac tttacatata
     2941 aacggggaac ttaggggatg tattggttat ccagaaccgg taaaaccact tttaaatgcc
     3001 ttaatcgagg tggctattgg ggcggctact ggtgatccac ggttttctcc tctaaattct
     3061 catgaactgg aaaaaattca cgtggaagtt agtgtactca ctccgccaga actaatagtt
     3121 actgatagac cttcggaata ccttgaaaaa atagatgtag gccatgatgg cctgattata
     3181 gaaaaagggt tattccgggg acttttactc cctcaagttc cggttgagtg gggatgggat
     3241 acggaggaat ttttggccaa cacttgcttg aaggcaggga tgactcctga ttgttggtta
     3301 caggaaggag ttaaaatcta ccgcttccag tctcagatct tcaatgagtg agaatttttc
     3361 aaggaatgga tacttaactc attaatccat gaatttccac ataatccatt aatcatatcc
     3421 aatgatagaa tagtataata aaaaataacc agatactata catatctatc caattcattt
     3481 tcaggatcat tatttgctgt taatttaata tttcttgaaa aaaatttgta gtataggaaa
     3541 gtgatttcat gtttttacca accataccca cgccagatga agtggtggat aaggcatttc
     3601 ggcgggcaaa aaaggcggct atgagaattc gaggttcaaa aatacgtgga cagaaaaaat
     3661 ctaaacgaat cgaggaggta agggtgcaga cggcttgtca agttattagg gatacctacg
     3721 atgacatcct aaaaaaaact cctcgagttg aggaactatc tgagttctac caggactaca
     3781 tagatgtggt agtaggggta gatcaattaa aaaaatcttt aggggcactt aaatgggcca
     3841 gtgatctttt agaaaaactt cagggtcagt atacctctaa aataagaaga tcacctcctg
     3901 aaaatgcttc taaaatcaga cgtgaagctt atggacggat tgtatcggtg gtcaatagaa
     3961 tcgagaatga actggacttt ttaacctttg ccaaacagca gctgcgt
//
LOCUS       DAOQVW010000081         4004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25330_length_4004_cov_3.117498, whole genome shotgun sequence.
ACCESSION   DAOQVW010000081 DAOQVW010000000
VERSION     DAOQVW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25330_length_4004_cov_3.117498"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..808)
                     /locus_tag="PKK85_02650"
     CDS             complement(62..808)
                     /locus_tag="PKK85_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNS24951.1"
                     /translation="MKVIFLGTNGWYDTCTGNTVCTLLETNDYYIVLDAGNGIYKLDE
                     YLKDTIKPIYLFLSHFHLDHIEGLHILNKFNFDNSLHIYGQPGTRKRLNKIINYPYTV
                     PFSELPFEVHLHEIGEGVHHLPFKVRCKFLPHASPCLGYRFEVDERIISYCTDTGPHK
                     NVEELGYKSDLLITECSLKMGESYPGWPHLNPFDAAKIALKAQAQRLALTHFDANIYR
                     THEERLESKESMKKLYKNIIYAYDGLEIKL"
     gene            complement(846..1820)
                     /locus_tag="PKK85_02655"
     CDS             complement(846..1820)
                     /locus_tag="PKK85_02655"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNS24952.1"
                     /translation="MNHEFWKDRNVFISGCTGLLGSWLTKKLVHNGANVIGLIRDAVP
                     KSNLNWSGFQDEIVTVRGEIEDYFVLERILNEYEVDCVFHLAAQTIVTIANRNPLSTF
                     EANIKGTWNILEASRRSPLVKRIVVASSDKAYGSQEKLPYSEETALEGKHPYDVSKSC
                     ADLLCGAYHQTYDLPVCITRCGNFYGGGDLNFNRLIPGTIRSILECKKPIIRSNGQMI
                     RDYFYIEDGAEAYMLLGEKMDKKIHGEAFNFSNEEPMTVLEMVNTILKVMNSDLKPII
                     LNESSNEIEKQYLSSRKAHTVLGWKSHYKLEEGLEKTIQWYQNFLSIN"
     gene            complement(1835..2611)
                     /gene="rfbF"
                     /locus_tag="PKK85_02660"
     CDS             complement(1835..2611)
                     /gene="rfbF"
                     /locus_tag="PKK85_02660"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNS24953.1"
                     /translation="MKVVILCGGKGTRLREETEYRPKPMVKIGEKPILWHIMKIYAYH
                     GFTDFVICLGYKGDMIKEYFLNYEIMNNDFTIDLSNKGKLDIHNRNGNDDWKVTLADT
                     GEDAQTGARVKKIKKYIDGDTFMLTYGDGVARIDIQDLVQFHQKHGKIGTMTGVHPSS
                     RFGEFSVEKNKITRFSEKPQTKADLINGGFFVFNNGFFDYLSDADDCIMEQAPLENLA
                     SDGNLMLYYNEGFWQCVDTYRELENLNEYWKRKNPPWKVW"
     gene            complement(2684..3574)
                     /locus_tag="PKK85_02665"
     CDS             complement(2684..3574)
                     /locus_tag="PKK85_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNS24954.1"
                     /translation="MGLKENDKGTIESPDSHKLLEQHKKLLQLSNLKIETLKVEKSEV
                     HFTRKMGIILLLYALSLVAAEIAITTVKPSWSLLFLIVIITSLVVLSSLSASAKFSHL
                     LQALILIPLLRIMSLSISMTEIGPVYWLALIIFPLIVSCIFLMRGQQLSISDVGLNLK
                     KLPVQLVIIWVGLAVGFIEYILLKPATITPNLDMPYMILSVIVLIGSGFILELIFRGI
                     IQQKADDLMGKLGGLLFLSTLFTTQYLAWNSVPYLIFVFGVSIIYGYVFQKTSSILGI
                     SISHGLANLMVFLILPLSVI"
BASE COUNT         1184 a          760 c          703 g         1357 t
ORIGIN      
        1 cgcgtcttat ctgttcttcc attttactcc ccaaaaccgt tttttttggt tatacataca
       61 gttatagttt aatttccaga ccatcatagg cgtatatgat gtttttgtat aatttcttca
      121 tggactcttt agattccagt ctttcttcat gggttctata gatattggca tcaaaatggg
      181 tgagggctaa tctttgggcc tgggctttta aagctatttt agcagcgtca aaggggttta
      241 aatggggcca accaggataa ctttctccca ttttcagaga acactcggtt atgagtaaat
      301 cagatttata acccagctcc tccacatttt tatggggtcc ggtatcagtg caataactta
      361 tgatcctttc atccacctca aaacgatagc ctaaacatgg tgaggcgtgt ggcagaaatt
      421 tacatctaac tttaaatggc agatgatgaa cgccttctcc aatttcatgt aaatggactt
      481 caaagggtaa ttcgctgaag ggaacagtgt aaggatagtt aatgatcttg ttcaatcttt
      541 tgcgggttcc aggttgtccg tagatatgga ggctattatc aaaattgaat ttattcagta
      601 tgtgcaggcc ttcaatatga tccagatgaa aatggcttaa aaagagataa ataggtttta
      661 tagtgtcttt taaatattca tccagtttat aaatcccgtt tccagcatcc agcacgatat
      721 aataatcatt ggtctccagt agggtgcata ctgtatttcc ggtgcaggta tcataccaac
      781 catttgttcc caggaaaatg actttcataa gatgaccttt cttacattag taatactcta
      841 gtgtattaat tgatggataa gaaattttgg taccattgga ttgttttttc gagtccttct
      901 tccaatttgt agtgagattt ccaccctaat acagtatgag cctttcgaga ggataaatac
      961 tgcttttcta tttcgttact ggattcattg agtataattg gcttcagatc gctattcatg
     1021 acctttaaga tggtgttaac catttccagt actgtcattg gttcttcgtt actgaaatta
     1081 aaggcttctc catggatctt tttatccatt ttttctccca gcaacatata agcttcagct
     1141 ccatcttcga tataaaaata atcccttatc atctgcccat tactgcgaat aatgggtttt
     1201 ttacattcta atatggatct aatggtcccc ggaatgagac gattaaaatt cagatctcct
     1261 cctccgtaaa agtttccaca acgagtaatg cacacaggaa gatcataggt ctggtggtat
     1321 gctccgcata aaagatcagc acaactcttg gaaacatcgt agggatgttt tccttctaga
     1381 gctgtttcct cactatatgg gagtttttcc tggcttccat atgctttatc actggaagct
     1441 accacaattc tttttacaag aggagatctg cgagaggctt ctaagatgtt ccaagtacct
     1501 ttaatattgg cttcaaaggt agagagaggg ttgcggttgg caatggtaac gatggtctga
     1561 gcagctaaat ggaatacaca atctacttca tattcattga ggattctctc cagaacaaag
     1621 taatcttcaa tttcgccccg cacagttaca atttcgtctt gaaatcctga ccagttaagg
     1681 ttagattttg gtaccgcatc acgaattagt ccaataacgt tggcgccatt atgtaccagt
     1741 ttttttgtaa gccaagaacc taaaaggcct gtgcatcctg aaatgaagac gtttcgatct
     1801 ttccagaatt cgtggttcaa tcatcttcct ccttctacca aaccttccaa ggaggatttt
     1861 ttcttttcca atattcgttt aaattttcca actccctgta ggtgtccaca cactgccaaa
     1921 aaccttcatt ataataaagc attaaatttc cgtcacttgc cagattttca agtggagctt
     1981 gttccatgat acaatcgtca gcatcactca gataatcaaa aaaaccgttg ttgaaaacga
     2041 aaaatccacc atttattaga tcagccttag tctgtggttt ttcactgaat ctagtaattt
     2101 tatttttttc cacagaaaat tctccaaacc ttgaagatgg atgaaccccg gtcatagttc
     2161 ctattttgcc atgtttttgg tggaactgta ccagatcctg aatatcaata cgggccactc
     2221 catccccata ggtgagcatg aaggtgtctc catcaatgta tttcttaatt ttctttaccc
     2281 tggccccagt ctgggcatct tccccggtat cggctagggt gactttccaa tcatcgtttc
     2341 cgtttctgtt gtgaatatct agtttacctt tgttggataa gtcgatggta aaatcattgt
     2401 tcatgatttc gtagttgagg aaatattcct taatcatatc tcccttatat cccaaacata
     2461 tcacaaaatc tgtaaaacca tggtaagcat agattttcat tatgtgccaa agaattggtt
     2521 tttcacctat ttttaccatt ggtttgggac gatattctgt ttcttctctc aacctggttc
     2581 cctttccccc acaaagaatc acaaccttca ttctcattcc tccatacctt ttataagatt
     2641 tattcataca ctttcttaat gattcatttt ttcatgtttt ccattatatt acagataagg
     2701 gcagaatcag aaataccatt aaatttgcta gtccgtggga gatgcttatt cctaatatac
     2761 tgcttgtttt ttggaaaaca tatccgtaaa tgatggaaac accgaaaacg aagattaagt
     2821 agggaacaga attccatgca agatactggg tggtgaaaag tgttgacagg aataacagtc
     2881 cccctaattt tcccattaaa tcatcggctt tttgttggat tatcccccta aagatcaatt
     2941 cgagaataaa tccagaacct atgaggacaa tcacgcttaa gatcatgtaa ggcatgtcta
     3001 aattcggtgt gatggttgct ggtttcaata atatatactc gataaatcct accgccaatc
     3061 ccacccaaat aattacaagc tgtaccggca gttttttcag gtttaaacca acatcagata
     3121 tactaagttg ctgaccacgc attaagaaga tacatgaaac gattaatgga aatattatta
     3181 gggctagcca atatacaggc cctatttctg tcatggatat acttaaactc ataattctca
     3241 ggagtggaat taaaatcagg gcttgtaaga gatgggagaa tttggcggag gcagataagg
     3301 atgataggac aactagtgat gtgatgatta ctatcaaaaa taataagctc catgatggct
     3361 taacagtagt tatagcgatc tctgctgcaa ctaaggagag tgcatagaga agtagaatta
     3421 tccccatttt ccttgtaaaa tgaacttctg atttttctac tttcaaagtt tctattttca
     3481 gattactcag ttgtaggagc tttttatgct gttcaagtaa tttatgggaa tctggactct
     3541 ctatagtccc tttatcattt tcttttaatc ccataatagg tctcctacct ttatatgctg
     3601 atcatagatt aattgcaatt tttgatttga agttttcagg agtgtagttg tatcttatta
     3661 agtcatgatt cataataaat attgtgacat ttcaacagga tgctaatgtg tgcacaattt
     3721 tttatttgct aaaatatttc attaagttaa ttttcccagt caaaatgaac atttaatgat
     3781 tgtttaattt ttaaaataat agaataactc aattatgtga gaaataaaca tcaaattgaa
     3841 taggtggtta taaaattagc gatgtatgca actaaacata tcaaaactag taacaatgga
     3901 ataattatca tatttaagct ggtgtcaaaa tttgctaatt tagtactttt atgagtcttg
     3961 gaactcacaa ttacttttag ggccaagatt agaaggagaa aaat
//
LOCUS       DAOQVW010000082         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25374_length_3999_cov_3.346856, whole genome shotgun sequence.
ACCESSION   DAOQVW010000082 DAOQVW010000000
VERSION     DAOQVW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25374_length_3999_cov_3.346856"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..695
                     /locus_tag="PKK85_02670"
     CDS             216..695
                     /locus_tag="PKK85_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24955.1"
                     /translation="MKRVTVTVAEEVDLKFKKEASKRFKFERGWYSAAVNEAMKAWLS
                     EDPSFDGQNFPRQLGKYLWINFRKKFDVHQMEPNECMNLLIEYIYGENHPIFYEISND
                     DVIINFSGIPEPENLEHHLLKMFQLITVVRAAQEDHKGSKYSISGLEEVKKIVISKE"
     gene            1198..2778
                     /locus_tag="PKK85_02675"
     CDS             1198..2778
                     /locus_tag="PKK85_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNS24956.1"
                     /translation="MVLVLAFMILWSLIWPLITSHALDAASTWVLVPQILLNFVLVFF
                     IISTIIYSAGYFKRIPVSLIVISGIIQLVTTIAFGCFTILNMYRGGILDLGWVTASIF
                     LVVAAVYQVDKKVSGTVTAPDWPYNVPSVNYLISALTVLGLILIMWGYILNKSLFTAF
                     LIGGGVLVLLSMFRQFVTFKLFETNWKKLRSSEQKYRDLFKTMAQGVVYQDTDGHIIS
                     ANHAAQKILGFTLDEITFTDPSWKTVNDVSDFRAEEHPSMVALKTGKIVKNVIRRAYN
                     PEKEKYVWIKINAIPKFRSGENKPYEVHCIFEDITELINSKEQIRKSLEKKEILIKEI
                     HHRIKNNLQIVSSLINLQSMKAESYEAATALDESENRIRAIALIHEKLYKSENLAHIR
                     LRDYIQDLASSIFRFYGVNPHRIQKKLDVDNLKIDIDSAVTLGLILNELISNSLKYAF
                     PNGEGEIRIELKIKHNQYILTVADNGIGLPKNFNLDQVQTLGIQLVIRLVNQLEGAIS
                     VENFTGTRWRITFPNKYD"
     gene            2846..3631
                     /locus_tag="PKK85_02680"
     CDS             2846..3631
                     /locus_tag="PKK85_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS24957.1"
                     /translation="MRKKILIVEDESVTSFEMELQLEAWGYEVVGTANSADMAIMQAR
                     KFSPDLMILDIRLKGELDGVYAAKQITKEINIPVIYVTAIPNETTIKQALKTNPYGYL
                     IKPYDKKELKFTIEMALYRHQILENLKTGLKSCHELYISPPEIIIILDKDGLVRYAND
                     STLKLVGLKIEEIWGRSITEVVPEEVVRKTCESAKLALSTSENISDQLKFSNGEKVCY
                     LNYHLFKLNTGKDDECYLVIGRDMTNKKDLEENIQKSVAEYLN"
     gene            complement(3797..>3999)
                     /locus_tag="PKK85_02685"
     CDS             complement(3797..>3999)
                     /locus_tag="PKK85_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS24958.1"
                     /translation="DENTAGSLYQSLKKEKKISYASFDRALKKLELLRIIDTRFTGKG
                     VKGNSRLVILRFSPEEMGKFLG"
BASE COUNT         1358 a          695 c          763 g         1183 t
ORIGIN      
        1 ccccccctgg gatggaatga atattgccat ctaaggacaa tattcacaat taccactatg
       61 gtttattatg cataaataca aactttaatt acagagaagc attgttgtgc tttaccaata
      121 aatttaaata atataattga gtggcaattt agtgatccat gattatggtg atcaaaagta
      181 aatattccat tagagggata atggttgggt tactgatgaa gagagttact gtaacagtgg
      241 cggaagaagt agacttaaag tttaaaaaag aagcatcaaa aagattcaaa tttgaacgag
      301 gatggtatag tgcagcggta aatgaagcca tgaaagcgtg gctatctgaa gatcctagtt
      361 ttgatggaca gaatttccca cgtcaacttg gaaagtatct atggataaat tttcgaaaga
      421 aatttgatgt tcaccaaatg gaacccaatg aatgtatgaa cctgttaatt gagtacattt
      481 atggagaaaa tcatcccata ttttatgaaa tttccaatga tgatgtaatt atcaattttt
      541 ctgggatccc tgaaccagaa aatctggaac accacctctt aaagatgttt cagttaatta
      601 ctgtagtgcg tgctgcccag gaagaccaca aaggttcaaa atacagtata agtgggctgg
      661 aagaagttaa aaaaatagtc atcagtaaag aataggatga atctaggatg cagaagatag
      721 ttacctaaac acttcattta gtagttctag attatgatga taatgtcgaa aatttttaat
      781 aaaaaaaaat attccaacac cctgtaccta gcttatatca tatttatctt atcctactct
      841 atattcctgt ttttcttttt ttccagtact cttgaaggct tgaagtacgc caacaacctt
      901 acagtaatgg ttgttaactt gctaaccttt ttgatactag tttacaccgc cataaacacg
      961 gggtatctgg agaaaaaata tcattatgtc tggatattct tagctatatc ccaactgttc
     1021 tgggtattgg gggatcttat gtggataaat tatgattcat tttcaagaga ctcctttgtt
     1081 aattggatgt acctggccta tagctttagg acattttttt tgtgtttatc cctgtattta
     1141 attcctaaaa gaaagataaa tcgtgacaca cactacaaga taacaataga aattttgatg
     1201 gtgcttgttc tggcatttat gatcctctgg tccctgatct ggcccttgat aacatcccac
     1261 gcattagatg cagccagcac atgggtacta gttccacaga ttctacttaa ttttgtactg
     1321 gtgtttttca taataagcac cataatctat tctgctgggt actttaaaag gatcccagtt
     1381 tctttaattg ttatcagtgg gataatccaa ctggtgacca ccatagcttt cggatgcttc
     1441 actatcctaa atatgtaccg tggcggaatt ctggatctgg gctgggtcac agccagcata
     1501 ttcctggttg tagctgcagt ttatcaggtt gataaaaaag tttctggaac tgtgaccgca
     1561 cctgattggc cttataacgt gccctcggtg aattatttaa tctccgccct gacagtcctg
     1621 ggattaattt taattatgtg gggttatatc cttaacaaat cactttttac agcatttttg
     1681 ataggtggag gagttctggt attgttaagt atgttccgcc aatttgtaac atttaaatta
     1741 ttcgaaacta actggaagaa actcagaagt tcggagcaga agtatcgtga tctgtttaaa
     1801 accatggcac agggggtagt ttaccaggat acggatgggc atatcatttc agcaaatcac
     1861 gctgctcaaa agattttggg attcacattg gatgaaatca ccttcactga tccatcctgg
     1921 aaaacagtta atgacgtatc cgattttaga gcagaagaac atccctcaat ggttgcttta
     1981 aaaacaggta aaatagtcaa aaacgtgata agaagagcat ataatccgga aaaggagaaa
     2041 tatgtgtgga ttaaaataaa cgcaattcct aaattcagat ctggtgaaaa taagccttat
     2101 gaagttcact gtatcttcga agatataact gagcttataa attctaagga acagattaga
     2161 aaatcactag aaaaaaaaga gattctaata aaggagattc atcatcggat aaaaaataat
     2221 ttacagattg tttccagtct tataaatctt caaagcatga aggcggagtc atatgaagcc
     2281 gcgactgccc tggatgaaag tgagaataga atacgggcca ttgccttaat ccatgagaaa
     2341 ttatataaaa gtgaaaattt ggctcatata agattaagag attatataca agatttagca
     2401 tcatccattt ttcgtttcta tggagtgaat ccacatcgga tccagaaaaa attggatgtt
     2461 gacaatctaa aaatcgatat tgactcagca gtaactctgg gtctgatttt aaatgaatta
     2521 atttccaact ccctcaagta tgcatttccc aatggcgaag gggagatcag aattgaactg
     2581 aaaattaagc acaaccagta cattctaacg gtagctgaca atggaattgg acttccaaaa
     2641 aactttaacc tcgatcaagt acaaacttta gggatccaat tagtcattag actggttaat
     2701 caattagaag gcgccataag tgtggaaaat ttcactggaa ctaggtggcg gattactttc
     2761 cccaacaagt atgattagtg cagaaaaata agtagagaga tattattaat tatgagtatt
     2821 aatataacat cgtatggaga ttcacatgag aaaaaagatc ctcatagttg aagatgaaag
     2881 tgtaacttcc tttgaaatgg aattacaact agaagcgtgg gggtatgaag ttgtagggac
     2941 tgcaaattca gcagatatgg ctatcatgca agcaagaaaa ttttcaccag atcttatgat
     3001 actggacatc cgtcttaaag gtgaattgga cggtgtctat gcagcaaaac agattactaa
     3061 agaaattaac atacccgtaa tttatgtaac ggccatccca aacgaaacca ccatcaaaca
     3121 agccttaaag accaacccct acggttacct gattaaacca tacgacaaga aagaacttaa
     3181 atttacaatt gaaatggccc tttaccgaca tcaaatcctg gaaaacttga aaacagggct
     3241 aaaaagctgt catgaactgt atatttcccc tccagaaatt atcataatcc tggataaaga
     3301 tggtttagtt cgttacgcaa atgattctac tctgaaatta gttggattaa aaattgaaga
     3361 gatatgggga agatccatca cagaagttgt cccggaggaa gtagtgagaa agacttgtga
     3421 aagtgcaaaa ctggcactgt ccacttcaga aaacattagc gatcaactaa agttttctaa
     3481 tggagaaaaa gtttgctatc ttaattatca cctttttaag ctaaacactg gaaaggatga
     3541 tgaatgttat ctggttattg gtcgtgacat gaccaataaa aaagatctag aagaaaatat
     3601 ccagaaatct gtagcagaat atcttaatta gttaactttc ctgtaacacg ctgttccagc
     3661 agctcagaac agtctttacg tgatactaat atacggtgct tcaattgatg ctaagaccca
     3721 gtgattgtgc aaaggcatta tgcaagtaat aatgagaata ttagtgtaga tgagtaattg
     3781 cactgaaatt aaaccatcac cctaaaaatt ttcccatctc ctccggacta aaacggagaa
     3841 ttaccagccg cgaattccct ttaacccctt tacccgtgaa ccgagtgtca attatcctta
     3901 aaagttctaa ctttttcagg gcacggtcaa aagaggcata gctaatcttt ttctcctttt
     3961 tcagggactg gtacagagaa ccagccgtgt tctcatcat
//
LOCUS       DAOQVW010000083         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25377_length_3999_cov_3.211460, whole genome shotgun sequence.
ACCESSION   DAOQVW010000083 DAOQVW010000000
VERSION     DAOQVW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25377_length_3999_cov_3.211460"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            698..2023
                     /locus_tag="PKK85_02690"
     CDS             698..2023
                     /locus_tag="PKK85_02690"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNS24959.1"
                     /translation="MISKNEIMETVLENDVEFIRLWFTDLNGILKSFAITNDELENAI
                     DRGMGFDGSSITGFQDVEESDMIAMPDLNTFTILPWRPIEKAVGRMICDVMQPNGDPY
                     EGDPRYVLKRALARMDKMGFDHFYVGPELEYFYFKSPENPEPLDHGGYFDLTPRDLAS
                     DLRRDTVLALKNLGIRVEYSHHEAAVSQHEIDMRYDDALKMADNMVTYRLAVKEIARN
                     HGVYATFMPKPIFNEYGSGMHTHQSLFKGDKNVFFDSDDPFHLSSYAKSYLGGILKYS
                     KEMISILAPWVNSYKRLVPGYEAPVYVAWSRNNRSALIRVPQYQPGREYATRIEVRCP
                     DPSGNPYLQLAVMLMAGLKGIEEKCALPEPMELNLYELNDKERIKREIDILPSSLEQA
                     IIYAEDSDLVKETLGPHSFRRFIALKKSECEEYNRQVTDYEIKKYFPIL"
     gene            complement(2838..>3999)
                     /locus_tag="PKK85_02695"
     CDS             complement(2838..>3999)
                     /locus_tag="PKK85_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNS24960.1"
                     /translation="LLPIHTAPWKPLWFTFVLRGADGVTMDTLYLRYNGTGFQVGNGT
                     NNPQISDIGPKALNNSTTVSYLNSKIFVDKNYFNIQSIANAWRNDPAFDQLMTFLFHD
                     HACPGVQPGFFISDYVFKNYPLSGDQNYYYMGSSIYCKDDSLVYLMGVSPGMGTYMNQ
                     RLMAEDTESEVIPGGTEEGLLVIWDPKTKTGKAVIINFKWATLNLTGLTTSDAQREAM
                     ISAYVSMYKGEPSPYVTQGVIAIASDERLITESEFNIIKQGGTASTTALSYVKGLPVR
                     SLSDLLPAQGGSQTGGSTQIPKGDSGTSTGDHTGSASGSVGDSGAAVSAVTVSAAVGE
                     EAGDSGKAYEVTQAGSEGAGNSPWGTAAIVGVIAVLALGAFGFLFKSGFLGK"
BASE COUNT         1173 a          795 c          801 g         1230 t
ORIGIN      
        1 tgatcatggt acactccaaa tttatccata ataacctgca gtcgatgaca gataaacgtt
       61 tgcctaagat taattatata gatttataaa gaattttagg ggttgagatt tttcaagttt
      121 cggagagctt ttgtagggct ttatcgtgct gtaactttgt gttgtgatgg atcctgctaa
      181 aataaagtta gtatggtcgt tatttccgtt gataggcttg tttatttgtc atttcttcat
      241 gtttattcgt ataatttgga aaaatcttca attttaccct gaagatcttg gaatagtgtg
      301 caactccaaa atggaggagt atatttggat ttttgagaac cgataatttt aaatattttt
      361 tttatacaag atataatttg ggtcttcttt actctaattt aagtgatact tcattatttt
      421 caccaagcaa gtatatacaa acatttatac tcaaatttaa cggtttatga gcccagttat
      481 aatcatataa tggaaataaa aatgtatttt tataaaaccc acatcttaat gaattataaa
      541 tatataaaaa aagaaaaaat agtccttttt tgtaattaaa acatctaaag ggctaaagaa
      601 tttttaacac aatatcaaaa aactataata gaaataatag taacaaatat tgatatatgt
      661 atacatttta ctattatatc aggaaaatgg tgctaatatg attagtaaaa atgaaataat
      721 ggaaacagtt ctagaaaatg atgtggagtt catcagactg tggttcactg atcttaacgg
      781 tattctgaaa agctttgcca taaccaacga tgaactagaa aatgccatag atagaggtat
      841 gggctttgat ggatcaagta ttactggttt tcaggatgtt gaagaatcag acatgattgc
      901 catgcctgac ctaaacacct ttactatttt accatggaga cctatagaaa aagccgtagg
      961 tcgtatgatc tgtgatgtga tgcaaccaaa tggtgatccg tatgaaggag acccccgata
     1021 cgttcttaaa agagctctgg cgcggatgga taagatggga tttgatcact tttatgttgg
     1081 gcctgaactg gagtacttct atttcaaatc tcccgaaaat cctgaaccac tcgatcacgg
     1141 cggatacttt gatttaactc cccgtgacct ggcatctgat ctccgtagag atactgtcct
     1201 ggctcttaaa aaccttggaa ttagggttga atactctcac catgaggcag cagtatcaca
     1261 gcacgagata gatatgcgct acgatgatgc ccttaaaatg gccgataaca tggtaaccta
     1321 tcgactggca gtgaaggaaa ttgccaggaa ccatggagta tatgccactt tcatgccaaa
     1381 acccatcttc aacgagtatg gatcaggcat gcacactcat cagtcacttt ttaagggtga
     1441 taaaaacgtc ttctttgatt ctgatgatcc attccacctt tcaagttatg ccaagtctta
     1501 cttaggcgga attcttaagt actccaagga aatgatatct atcctggctc cctgggtgaa
     1561 ttcctataaa agactggttc ctggttacga agctccggtt tacgtggctt ggtcacgaaa
     1621 caatcgctct gccctcatca gagttcccca gtatcaacca ggccgggagt atgctacccg
     1681 aatcgaagtt agatgtccgg atccctcggg aaatccctat ctacagttgg cggtgatgct
     1741 catggcgggt ctgaaaggaa tagaagagaa atgtgccttg cccgagccaa tggaactcaa
     1801 tctctatgaa ctcaatgata aagagcgaat taaaagggaa atagatattt taccatcaag
     1861 tttagaacag gccataatat atgctgagga ttcagatttg gtgaaggaaa cccttggccc
     1921 acattccttc cgaagattca ttgccctaaa aaaatcagaa tgtgaagaat ataaccgcca
     1981 ggtaaccgat tatgaaataa aaaagtattt ccccatactt taatttttaa agagttttct
     2041 caatatcaag ccagagcaac ctatttgaga gttatggagg aagatggttt ccttttcctc
     2101 cacaacttca ccaagatttt tcagatgcta atgccagttt ccaaatctag ttgattaaga
     2161 aaaacagtca tttttaggag tcgtttatca ccatcccccc agaaccttat ctgtacatca
     2221 ttttcgaaaa gaaactccct cttttcaata ttggatcagt ttcatacgaa ctaactcctg
     2281 tttctccaat ttcaggggtt gattgctctt ccatgtttta tccggagtta cttaaaaaaa
     2341 tagatatctc acggagtatc agtggggcac tccgtaagat aatgaggtgt tcaaacccct
     2401 ttttaggtat ttacaagctt ttaaatatcc actgttcccc agggatggta gggggaaaaa
     2461 tatggcaaag gacgaaggtt ttgagcacat atttaagccc ttataattta gattgttttt
     2521 ctcctcgtcc agattaaaca ctgtaccacc acctatataa taatttctat ttaattcgta
     2581 ttattacgtt gtggctaaaa tgaccttttt tttattacgg atgatcagcc tctgcctatt
     2641 aacttatccc atagaatcct gatggaagtt ccatttcata caaattcccc ataattttaa
     2701 ctcttttagt ggatttaatt caattacgcc tatccccatg tgcattggca taggctatat
     2761 aacagtatat ctaaaataag ggagaattat ctcttcaaat agtgttttaa aaaaaaagaa
     2821 aaaggggata aatgatttta ttttcctagg aatccgcttt tgaacaggaa tccaaatgca
     2881 cctaaggcaa gaacagctat cactcccacg atagccgctg taccccaagg tgaatttcca
     2941 gcaccttcag agcctgcctg ggttacttcg taggcttttc cactgtcacc agcttcttct
     3001 cctacggcag cactcacagt cacagcacta actgcggctc cagagtcacc caccgaacca
     3061 gatgcagagc cagtatggtc acccgtactg gttccagaat cacctttcgg tatctgagtg
     3121 gaaccgccag tttgggaacc gccctgagcc ggtagaaggt cactgaggct gcgcactgga
     3181 aggcctttca catagcttaa ggcagtagtg ctggcagttc cgccctgttt gatgatgttg
     3241 aattcactct cagtgatgag tctttcatca gaagctatgg cgatgacacc ttgagtcacg
     3301 taaggacttg gttctccctt gtacatactg acataggcag atatcatggc ttctctttgg
     3361 gcatcactgg tggttagacc agtcaagttt aaagtagccc atttgaagtt gatgatcact
     3421 gctttgcctg ttttggtttt ggggtcccag ataaccagta atccttcttc tgttccacca
     3481 ggtataacct cagattcggt atcttcggcc attagtcttt ggttcatgta ggttcccata
     3541 cctggggaca ctcccatgag gtagaccagg ctgtcgtctt tacagtatat actggatccc
     3601 atgtagtagt agttttgatc accacttaat ggataattct tgaacacgta gtcggaaatg
     3661 aagaatccgg gctgtactcc agggcaagcg tggtcatgga aaaggaaagt catcagctgg
     3721 tcaaatgcag gatcatttct ccaggcgttt gctatggact ggatgttgaa gtaattcttg
     3781 tctacaaaga ttttactgtt caagtatgaa actgtggtac tgttgttgag tgcttttggt
     3841 ccaatatcac tgatctgggg gttgttcgtc ccatttccga cttggaatcc ggttccattg
     3901 tatcgcaggt atagggtatc catggttacc ccgtccgctc ctcttagtac gaatgtgaac
     3961 cataatggtt tccagggtgc ggtgtggatt ggtaacagg
//
LOCUS       DAOQVW010000084         3996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25406_length_3996_cov_3.346359, whole genome shotgun sequence.
ACCESSION   DAOQVW010000084 DAOQVW010000000
VERSION     DAOQVW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25406_length_3996_cov_3.346359"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKK85_02700"
     CDS             <1..261
                     /locus_tag="PKK85_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24961.1"
                     /translation="GIPYDNSYSIEYQTLMAYDNIINSTILLGDAYVFFGKFNIDNKK
                     ILVADNDTGELYYLNDTRLENKIYKLYDNGDSWILKGTGVRT"
     gene            258..1319
                     /locus_tag="PKK85_02705"
     CDS             258..1319
                     /locus_tag="PKK85_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNS24962.1"
                     /translation="MIIGIIPPVTRTGEFKYCETLVRGLNERGLEVKVLNNQFLVHRS
                     NLKIFLGSLLLKRIIKEEVEILHNTDNLGPFLFRNSDTKIKRISTVHDIAPVVLPDIH
                     SNIMKFDFKIVLPRLIENSDLIITDSYSTKEDLEEYFQVNAAKIDVAHLGVDLSFFYP
                     REHDDILMKKYGINNDYIFYLGNDNPRKNLKNLIIAYSKIYKDVEQDLVLVGPIDQKN
                     LRTLTEKIDKGGDLSERIITPGYVDYEHLPLFYSQATSLIFTSLYEGFGFAPLEAMAC
                     GTPVIISNNSSISEVVGDSGLYINNPLNVEEISNKITELLNDEKLQNKLMKRGPKRAE
                     MFTWDNTINKTIKAYEKVY"
     gene            complement(1333..2355)
                     /locus_tag="PKK85_02710"
     CDS             complement(1333..2355)
                     /locus_tag="PKK85_02710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS24963.1"
                     /translation="MYPNVSIIVLNWNGWEDTVECLESLYQIDYPNYNVIVVDNGSQD
                     NSIQKIKDYCQGKLEIESDLFEIDVKKPIKIFEYFNDDIKDISSDLNLIQTLNSHKKL
                     ILIKNDDNYGFAEGNNIGMRFVLKNFDSNYLLLLNNDTVVKKDFLSKLVDLSENDAHI
                     GICGPKVLYYHQPHVINSAGGKIVWHMGLGINKGIGERDEGQYDAISETDSLLGACIL
                     INSDVIRKCGLLDHQFFLLLEETDFCIRAKKAGYKIFFNPQAVIYHKEGISGEISPLK
                     YYYMYRNRLFIIRKHQTPIKVLLYSLIVSLRTLFIIIYFAAKGDKNVLKSIFYGYLDG
                     IHEKIT"
     gene            2611..3102
                     /locus_tag="PKK85_02715"
     CDS             2611..3102
                     /locus_tag="PKK85_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24964.1"
                     /translation="MGVKKTVFKTFKFILKYRLLFFLILLILLGALSYYYSENYYLHE
                     EDPSSENFLLNYPDGHVVSLEGWVVDVYPGGFKLYIYHHGKDTIFDVRSNTNVSVGDN
                     VFVKGNLDDEKIITAKKVVLKQYWMYIFLLARSALAILIVIILLLRYWKFHLENWEIV
                     RRK"
     gene            3102..3581
                     /locus_tag="PKK85_02720"
     CDS             3102..3581
                     /locus_tag="PKK85_02720"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNS24965.1"
                     /translation="MPDWLTHVLVAWSLCTVLGFRYKEFNSENTVIAMLGALLPDAVK
                     IAIPIQFLSGHDPSTMVATIHTPLGSLILGAILSLLFKDKKTVFLFLLLGILTHFALD
                     LLMSYYSGGTYLLYPFSWNQWQIGLISTVDYKISLLTVIISIYVYLVARIKNSIDNS"
BASE COUNT         1366 a          649 c          680 g         1301 t
ORIGIN      
        1 ggaataccat acgataattc ctattcaata gaataccaga ctcttatggc ctatgataat
       61 ataattaact ccaccatcct tcttggtgat gcatacgtgt tctttggaaa gttcaatata
      121 gacaataaga aaatattagt ggctgataat gatactggtg aattatacta cttgaatgat
      181 actcgtcttg agaataaaat ctacaaatta tatgataatg gagattcttg gatattgaag
      241 ggcacagggg ttcgaacatg ataattggaa ttattccacc agttacccga actggagaat
      301 ttaaatattg tgaaacttta gtcagaggat tgaatgaacg cggattggag gttaaagtgt
      361 taaacaacca atttttagtt cacaggtcca acttgaaaat atttttaggt agtttactgt
      421 tgaagagaat tataaaagaa gaagttgaaa tcttacacaa tacagacaac cttggtcctt
      481 tccttttcag aaattctgat actaaaatta aaagaatttc aactgtacat gatattgccc
      541 cggttgtatt accagatatt catagtaata taatgaaatt tgattttaag attgtactcc
      601 ctagattaat tgaaaacagt gatttgataa tcacagatag ctattctaca aaagaagatc
      661 tggaggaata cttccaagtt aatgcggcaa aaatagatgt agcccatctg ggagttgatc
      721 tatccttttt ctatcccaga gaacatgatg atattttaat gaaaaaatac gggattaata
      781 acgattacat cttttattta ggcaatgata atcccagaaa aaacttgaaa aaccttatca
      841 tagcttacag caaaatttac aaggatgttg aacaggattt ggttttagtg ggtcctattg
      901 atcaaaaaaa tttgcgaact ttaacagaaa agattgacaa gggtggagat ttatctgaaa
      961 ggataatcac gccgggatat gtggattatg agcacttacc tctattttac agtcaagcaa
     1021 cgtcattaat attcacatcc ctctatgaag gctttggatt tgcacccctt gaagcaatgg
     1081 catgtggaac gccggtaata atttccaata atagttctat ttccgaggtg gtgggagact
     1141 caggtttgta tataaacaac cccttgaatg ttgaagagat atccaataaa atcactgaac
     1201 ttttaaatga tgagaaactc cagaataaat tgatgaaacg gggtccgaaa agagctgaaa
     1261 tgttcacctg ggacaacacc ataaataaaa caataaaggc ctatgaaaag gtttattaga
     1321 aagggtaaaa atttaagtga ttttttcatg aattccatct aaatatccgt aaaatatcga
     1381 ttttaaaacg tttttatctc ctttagctgc aaaataaatt ataataaaca gtgtccttaa
     1441 cgaaacaatt agactgtata aaagtacttt gattggtgtt tgatgtttcc tgataataaa
     1501 caatcgattc ctgtacatat aataatactt cagggggctg atttcccctg agatgccctc
     1561 tttatgataa atcactgctt ggggattaaa aaaaatttta taacctgctt ttttggctct
     1621 gatacaaaaa tcagtttctt ccagtagtaa aaaaaactga tgatctaaaa ggccacactt
     1681 cctgattaca tctgaattta ttagaataca ggcacccaaa agactatcag tttctgatat
     1741 agcatcatat tggccttcat ccctctctcc tatgccttta tttattccaa gccccatgtg
     1801 ccacactatt tttcctcctg ctgaattaat aacatggggt tgatggtaat aaagtacttt
     1861 tggcccgcaa ataccaatat gggcatcgtt ttctgataga tccactagtt tggataagaa
     1921 gtcttttttt acaacagtat cgttgtttag aagtaataaa taatttgaat caaaattttt
     1981 taagacaaac ctcatcccta tgttgttccc ttcagcaaat ccataattat cgtcattttt
     2041 aattagaata agttttttat gagagttcag tgtttgaatt aagtttaaat cactacttat
     2101 gtcttttata tcatcgttaa aatattcaaa tatctttatt ggctttttaa catcaatttc
     2161 aaataaatca gactctattt ctaatttccc ctgacaatag tcctttattt tttgaattga
     2221 attatcttgg gagccattat caactacgat cacgttataa ttaggataat ctatttggta
     2281 taacgattcg aggcattcaa cagtatcttc ccacccattc cagttgagaa ctatgatcga
     2341 aacatttgga tacattttgc catattctcc cttatactgt acagataatt gggatgaatt
     2401 tagaatctaa gatactcagg aattataagg aataatttat aatattgaat cagtgaatta
     2461 ggattgataa atcttacgaa tctctcttga gagcgaaata actgaaattc aatattcaat
     2521 tattgataga ctagctaatg gctgatagat gtacgtggaa aaataacaca gcaaataatt
     2581 tacataatta gggatgaata atatattaca atgggtgtta aaaaaacagt ttttaaaacg
     2641 tttaaattca ttctaaagta tagattacta ttttttctca tcctcctaat actattgggt
     2701 gctctaagct actactacag tgaaaattat tatctacatg aagaagatcc atcttctgaa
     2761 aactttctgc taaattatcc cgatggccat gtggtgagct tggagggatg ggttgttgac
     2821 gtataccccg ggggattcaa actgtacatc tatcaccatg gcaaagatac catatttgat
     2881 gtaagatcta acacaaatgt ctcagtaggc gataatgtat ttgtgaaggg caatttagat
     2941 gatgaaaaga taataacggc taaaaaagtc gttttaaagc aatattggat gtatatattc
     3001 ttactggcca gatctgccct ggccatttta atagtaatta ttttgttact tcgatactgg
     3061 aaattccacc ttgaaaattg ggaaatagta aggagaaagt aatgcccgac tggttaacac
     3121 atgtactggt agcatggagc ttatgcacgg ttcttggctt tagatacaaa gaatttaatt
     3181 ctgaaaatac ggtcatagct atgttaggag cactgctacc tgatgcagtg aaaatagcta
     3241 tccccataca gtttttaagt ggacatgatc catcgactat ggttgctacc attcacactc
     3301 cattagggtc tttgattctg ggggccatat taagcctcct atttaaagat aaaaaaaccg
     3361 tatttctatt tctcctcctg gggatcttaa ctcacttcgc cttagaccta ttaatgagct
     3421 attattcggg tgggacatac ttactttatc cgtttagctg gaaccaatgg cagataggac
     3481 ttatatcaac agttgattat aaaataagtc ttttgaccgt aataatttct atctatgtgt
     3541 atctggtggc acgtataaag aattcaatag ataatagtta atttaaccaa tttcattgat
     3601 catattctca ttttccggaa tatttcaaga tcatcactgg taaacgtttt caggagaatt
     3661 aatattacaa aataagaaat aatagcaata ataactgata aaaacaatcc actgttaagt
     3721 ttgaagataa tcaaacctga aatggcactg gcaacaacag gtttaatcat gatcttccca
     3781 atttcaattt tgcagataaa tttggaaagg aagtaaaatt caattggaaa actgaatagg
     3841 gtagttatca cagtggctaa tgcggcgccg atataactga attgcggaat caaaattaaa
     3901 ttcagaataa tgttagctat catcaggaca aatgttaatt ttagcagtaa attttgattc
     3961 cgcaattcag ttatatcggc gccgcattag ccactg
//
LOCUS       DAOQVW010000085         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25460_length_3989_cov_2.716065, whole genome shotgun sequence.
ACCESSION   DAOQVW010000085 DAOQVW010000000
VERSION     DAOQVW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25460_length_3989_cov_2.716065"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /gene="rpl2p"
                     /locus_tag="PKK85_02725"
     CDS             complement(<1..473)
                     /gene="rpl2p"
                     /locus_tag="PKK85_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875647.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNS24966.1"
                     /translation="MGKRLIIQRRGRGTPTYRSASHRFKGKIAYRYYDDVEKEGCLVG
                     KVADIMHDPGRSAPVALVKFENGEKQFILAPESIQIDDEIACGISAPISPGNSLPLGQ
                     IPEGTPVYNLERHPGDGGKFVRSSGTHASLITHDVGKAIVELPSGELKAFNPQCRA"
     gene            complement(487..747)
                     /locus_tag="PKK85_02730"
     CDS             complement(487..747)
                     /locus_tag="PKK85_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNS24967.1"
                     /translation="MDPYSIIIKPHLTEKSMNAIDQKNELTFVIRRTANRGQVKNAIQ
                     EMYEVKVERVNTQITSRGVKLAYVKLSEEDSAEDIAMKMGVF"
     gene            complement(757..1545)
                     /gene="rpl4p"
                     /locus_tag="PKK85_02735"
     CDS             complement(757..1545)
                     /gene="rpl4p"
                     /locus_tag="PKK85_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNS24968.1"
                     /translation="MKKVKVYSLEGEVVDEMELPEIFTEEYRPDLIKRAVLSAQAARI
                     QPWGTDPMAGKRTTAQSYGAGRGVAMVPRVKGSRHPAGSKAAFVPQAAGGRRAHPPRV
                     ARTITEKINKKERKLAIRSAVAATASQDLVEARGHQIGNVPQVPLVVDDEICSIKKTR
                     ETREIFKKLGLMDDVVRAKKGKNTRSGRGKTRGRKYKTPRGPLLVVGQDKGISKAARN
                     HPGVEVVVVDNLNAELLAPGTHPGRLTLYTTSALEKLGELFKNR"
     gene            complement(1577..2587)
                     /gene="rpl3p"
                     /locus_tag="PKK85_02740"
     CDS             complement(1577..2587)
                     /gene="rpl3p"
                     /locus_tag="PKK85_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNS24969.1"
                     /translation="MARHHQPRKGSVAYSPRKRAIREAPRVHSWPEREEPGLLGFPAY
                     KVGMTHVIMTDTEKNSPTEGMEVSTPVTVLEAPPVVVMGIRAYRKTDRGIKAMTDIMA
                     PELDSELSRKITLPRESSTDARLAELKEKLDQVANIRVLIHTNPKDASVPKKKPELLE
                     CGVGGTSVEAKLEYAETMLGKEVKPEDTFSDGEHTDAVAVTKGKGFQGVIKRWGVRIQ
                     YGKAARSSKGRHVGSIGPWSPERTMWTVPMAGQMGYHQRTEYNKKILRIGEAKDAGDI
                     NPAGGFVKYGLVKNDFIVVKGSLPGPSKRLVMLRKAARPHGKQDDVPQISNISTASQQ
                     GA"
     gene            complement(2716..3531)
                     /locus_tag="PKK85_02745"
     CDS             complement(2716..3531)
                     /locus_tag="PKK85_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA uridine N3 methyltransferase"
                     /protein_id="HNS24970.1"
                     /translation="MEEPQISIFIPSSFIGESKDLKVRTYKVGLISRSAALFRVNRIV
                     IYSDDQDRKGTKFISDVLNYMNTPQYLRKQVFPISRELRNVGILPPLRTPHHPTGELH
                     QGDYRQGLTVKRMKRGTLVDIGADRLALCKEKLSVNRVLNFKVEKLAKEILITPDEPD
                     VYWGYQTLATYRDLQDSLKALKPQPDLVVGTSRLGQPITSVFNEVKEDLKGSKHLAIL
                     FGGPYSGLHDLNGETRKVLDLELNTIPHQGTKTVRTEEAVLATLSLFNLLLDL"
     gene            complement(3810..>3989)
                     /locus_tag="PKK85_02750"
     CDS             complement(3810..>3989)
                     /locus_tag="PKK85_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24971.1"
                     /translation="NQANQYTSRENIQINSPIMMASAIIIGLLIWIFPLLVYWFAWLA
                     VFMLLIGGIAALVVK"
BASE COUNT          817 a         1040 c          923 g         1209 t
ORIGIN      
        1 gctctgcact gggggttgaa ggccttcagc tcgccggacg gcagctccac aatggcttta
       61 cccacatcat gggtgataag agaagcatga gtacctgagg accgcacgaa tttgccccca
      121 tcaccggggt gtcgctccag gttgtacact ggggttccct ctggtatctg tcccaggggt
      181 agtgagtttc ctggactgat gggtgcggat attccacagg caatttcatc atcaatctgt
      241 atactttctg gtgctaaaat aaactgtttt tcaccgtttt cgaatttaac cagggccacc
      301 ggggcgcttc ttcccgggtc gtgcatgata tcggccactt tccccacgag gcagccctct
      361 ttctccacgt catcataata ccggtaggcg attttgccct tgaaacggtg ggacgcactg
      421 cggtaggtgg gagttcccct cccccttctt tgaatgatca atctttttcc cattgtcttt
      481 tcctccttag aatacgccca tcttcatggc gatgtcctcg gcgctgtctt cctcggatag
      541 ttttacgtag gccagtttga caccgcggga ggttatctgg gtgttaaccc tttccacctt
      601 cacttcgtac atttcttgga tggcattttt gacctggcct ctgttagcgg ttcgccgaat
      661 cacgaaggtt aattcgtttt tttggtcaat ggcgttcata cttttttcag ttaagtgtgg
      721 tttgattatg atactgtaag gatccatgga agccacctac ctgtttttga atagttctcc
      781 taatttttcc agggcagagg ttgtgtacag ggtcagtctt cctgggtggg ttccgggtgc
      841 taaaagttcg gcgttgaggt tgtccaccac caccacctct actccagggt ggtttctggc
      901 ggctttgctt atgcccttgt cctgacccac taccagtaag ggtccccggg gggttttgta
      961 ctttcttccc ctggttttac cccttccgga tcgtgtgttt tttcccttct tggcccgtac
     1021 cacgtcgtcc atgagtccca gtttcttgaa gatctccctg gtctccctgg tcttttttat
     1081 gctgcagatc tcatcatcca ccaccagggg tacctggggc acgtttccta tctggtggcc
     1141 ccgggcctcc accaggtcct gactagcggt ggcagccact gctgagcgta tggccagttt
     1201 tcgttctttc ttgttgattt tctcggttat ggtccgggcc actcgtggtg ggtgggctcg
     1261 tctaccaccg gcagcctggg gcacgaaggc cgccttggaa ccagctgggt gtcgggaacc
     1321 ctttaccctg ggaaccatgg ccactccccg tccagccccg taagactggg cggtggtcct
     1381 ctttccagcc atgggatcag ttccccaggg ctggatgcgt gcggcttggg cagatagcac
     1441 cgctcttttt atgaggtcgg gcctgtattc ttcagtgaaa atctcgggta gctccatctc
     1501 gtctaccact tcaccttcta atgagtatac cttaactttc ttcatcccaa tcactttaaa
     1561 caaatgttac taataattag gccccctgtt gggatgccgt gctgatgttt gaaatttgag
     1621 gaacatcatc ctgtttacca tggggacgtg ctgcttttct taacatgacc agtcgcttgg
     1681 atggtccggg caacgaaccc ttaactacga taaaatcgtt tttcaccagc ccgtacttca
     1741 cgaagccacc agcggggttg atgtcaccgg cgtccttggc ttctccaatg cggagtatct
     1801 tcttgttgta ctcggttctc tgatggtagc ccatctggcc agccatgggt acggtccaca
     1861 tggttctttc cggactccag ggacctatgg aacccacgtg tcgtcccttg ctacttcggg
     1921 ctgcttttcc gtactggata cgaacgcccc atcttttaat aacaccctga aatccttttc
     1981 ccttggtcac agccactgca tcggtgtgtt caccatcaga gaaggtgtct tcaggtttaa
     2041 cttccttacc cagcattgtt tcagcgtatt ccagtttggc ttctacactg gttccgccta
     2101 caccacattc caggagttct ggtttcttct tgggtacact ggcatctttg gggttggtgt
     2161 gtatcaacac cctgatgttt gctacctggt ccagcttttc tttgagttca gccaggcgtg
     2221 cgtcggtgct gctttccctg ggtagggtga ttttacggga gagctccgag tccagttccg
     2281 gggccatgat gtcggtcatg gccttgattc cgcggtctgt tttacggtag gcccggatac
     2341 ccatcactac cacgggtggt gcctccagga ctgtcactgg agtggagacc tccattcctt
     2401 cggtggggga gttcttctca gtgtcggtca tgatgacgtg ggtcatgccc accttgtagg
     2461 cggggaatcc caacaatccc ggctcttctc tttcaggcca ggagtgtacc cggggtgctt
     2521 cccggatcgc tctttttcta ggactgtatg caactgatcc ttttcggggt tgatgatgtc
     2581 tagccattta aatttacctc cttacaaatt ccaaatttcc aataatataa cttttccaat
     2641 tagagttagg tctatttaac gatagtccaa cctactccta tccagggtag gctctttaaa
     2701 ccggacttaa gcgcttcaca agtctaagag caagttaaac aaagataaag tcgctaaaac
     2761 cgcttcttca gttcttactg ttttggttcc ctgatgggga atggtgttta actccaggtc
     2821 aagaactttc ctggtttccc catttagatc atgtaagcct gaataaggac caccaaacaa
     2881 aatagccagg tgtttggagc cctttaaatc ctcttttact tcgttgaaaa cagaagtgat
     2941 gggctgacca aggcgtgagg tgcccaccac caggtctggt tggggcttca atgcctttaa
     3001 actatcctgc agatcccgat aagtagccag ggtttggtaa ccccagtata catccggctc
     3061 atcgggagtt atcaggatct ccttcgccag cttctctacc ttaaagttta aaacccggtt
     3121 cacactgagc ttttctttac acagtgccag ccggtcagca ccaatatcaa ccaaggtgcc
     3181 tctcttcatc ctctttaccg ttaatccctg cctgtaatca ccctgatgaa gttctcctgt
     3241 ggggtgatgg ggagttcgta gcggtgggag tatgcccacg ttcctcaact cccgactaat
     3301 gggaaatacc tgtttacgca gatattgtgg ggtattcata taattgagga catcactaat
     3361 aaactttgtc cctttgcggt cctggtcatc gctgtatatt acaatgcgat taacacggaa
     3421 tagggctgct gatctgctaa tcagccctac tttgtaggtt cttactttta aatcttttga
     3481 ttcccctatg aatgaggatg gtatgaagat ggagatctga ggttcttcca tcttatatgg
     3541 tctatatata ttgggtaata tagttttgtg gagctaggaa aattgctcca atatacaata
     3601 tattaggatc taatgtccac atcctttacc tggccaacta ctagggcctt cacatccaca
     3661 acctctttcc ctatgatttc ttctttaatc cccatatccc ctgtaacagg gataacttta
     3721 cgggttgctt cttcccaggg taattgcctt gaactgttta agaattgcat aaaaaaagta
     3781 aggggcttta tttcaaaccc ctgtttcttt cacttcacta ctaaagcagc tatcccccca
     3841 attaacagca tgaatactgc taaccaggca aaccagtata ccagcagggg gaatatccag
     3901 attaacagtc ctataatgat agcacttgcc atcattatag gactgttaat ctggatattc
     3961 tccctgctgg tatactggtt tgcctggtt
//
LOCUS       DAOQVW010000086         3983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25520_length_3983_cov_3.116344, whole genome shotgun sequence.
ACCESSION   DAOQVW010000086 DAOQVW010000000
VERSION     DAOQVW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25520_length_3983_cov_3.116344"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKK85_02755"
     CDS             <1..781
                     /locus_tag="PKK85_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24972.1"
                     /translation="KPAEEPKKITPPNREEFKKITPPPREESKPPEQDENPPKVKTPP
                     KIEPEKKEAKPVKRTPVVPKKAPDSEVKDDSYVKNRLERLKDDYILNSKDIETLVDER
                     LDSFKGTLNRLKTETTDPSIIWSFEAQDVQGALKDTISQAEEKILMMYPWVRNIDVSI
                     LKKFMDTQSRMIIQEASLDDDTSVELIKLLLENQVQIRTMPHVHTVAVVADDKNGLII
                     STDPIYDSYEVGVVYRDEKSIQEIENMFEDAWNLSKEMKLE"
     gene            778..1473
                     /locus_tag="PKK85_02760"
     CDS             778..1473
                     /locus_tag="PKK85_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNS24973.1"
                     /translation="MRITWLGHSAFEIVTSEDLHILIDPFITENPACTCSLDEVQADV
                     ICVTHGHNDHFGDTLKLAERDGSLVICIHELSKYLECQGIEAWGMNMGGTVEARGVKV
                     TMVNSNHSSDMDFLEGMGPGGSSCGFILELEDGKKIYHSGDTGIFGDMKTVIRDIYSP
                     HLAMLPIGDRFTMGPHEAKIAVEWLDVDVVIPMHYNTFPVIEQDPEKFKEYVESTTGA
                     KVVVLEPGETYQE"
     gene            1478..2122
                     /locus_tag="PKK85_02765"
     CDS             1478..2122
                     /locus_tag="PKK85_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HNS24974.1"
                     /translation="MVNVVFKIFRRNYFRDIFYKLIGKNKKLKIGFYGHPNSGKTTLA
                     NRITTDWIGQSLGLVSEVPHETRRVYRQEHVTLKQDGMELDFDIIDTPGIATKIDYKD
                     FLEFGLSEEEAKERAKEATKGIIEAIKWLDDVTGVLLVVDATQDPLTQANITIMGNLE
                     ARKIPFLIVANKIDLPEASTERIKAVFPQHRVVSISALQGDNVEGLYQAMVNHF"
     gene            2134..2514
                     /locus_tag="PKK85_02770"
     CDS             2134..2514
                     /locus_tag="PKK85_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HNS24975.1"
                     /translation="MEEISNSLKMDFLSSDALKNRSSMEKIAMIVDKVKNGDLLVMEG
                     GLTPSEEAELIETTMREIDVENFLGIDIYTLEKDQSTFFGLSKRKTVGITIIGPANVM
                     KTVKRKSNFLSMIAQLGGADAPVH"
     gene            2498..2797
                     /locus_tag="PKK85_02775"
     CDS             2498..2797
                     /locus_tag="PKK85_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon containing protein"
                     /protein_id="HNS24976.1"
                     /translation="MHRCIKCHAEYESSEILLEGCPECGSKYFEYHQEGKPPKIREAR
                     GDSIETIMVHEHGVYEVDLPSLLGDDSIIVSDEEGKYLIDLNFLLKKGLKEKKRH"
     gene            complement(3068..3748)
                     /locus_tag="PKK85_02780"
     CDS             complement(3068..3748)
                     /locus_tag="PKK85_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNS24977.1"
                     /translation="MSDDYYLDFQNYPLHTWQEELKKASLLPSRQILKEDLDARFNIL
                     SRKGISNLQELMDALKTSRKVREFASQTGLPEDYLLVLRREVLSLKPGPVKLSKLPGV
                     KPELVEKLEAAGIKTTLELFKRAKTSQDRDELGEEVGLRREEIRELAKLSDLSRIKWV
                     GPVFARMFLEAGMDSVKKVSEADASEFYAELVNINQNHKHTKARFVENDLVMCIEFAK
                     KLPEVMEY"
BASE COUNT         1104 a          969 c          985 g          925 t
ORIGIN      
        1 aaaaccagca gaagaaccta aaaagatcac ccctccaaat agggaggagt ttaaaaagat
       61 cacccctccg cccagggagg aatctaaacc tccagagcag gatgaaaatc cgccaaaggt
      121 taaaactccc cctaaaattg aacctgagaa aaaggaagca aaacccgtga aaaggacgcc
      181 tgtagtaccc aaaaaagctc ctgattctga ggttaaagat gattcctacg tcaagaacag
      241 gctggaacgc cttaaggatg actacatcct caattccaag gacatagaaa cactggttga
      301 tgagaggctg gactccttca agggaactct gaaccgtctt aaaacggaaa ccacggatcc
      361 cagcatcatc tggtcatttg aggcccagga tgtccaggga gcccttaaag acaccatatc
      421 ccaggcagag gagaagatcc tgatgatgta cccctgggtg cgtaacatcg atgtatccat
      481 actgaagaag ttcatggaca cccagagcag gatgatcatc caggaagcca gcctggacga
      541 cgatacctca gtagaactta tcaaactctt attggaaaac caagtccaga tcaggaccat
      601 gccccatgtg cacaccgtgg cagtggtggc tgatgataaa aatggactta taatctccac
      661 cgaccccatc tacgatagct acgaggtggg cgttgtctac cgggatgaga aatccatcca
      721 ggaaatagag aacatgtttg aggatgcctg gaacctgtcc aaggagatga aactagaatg
      781 agaataacct ggctggggca ttctgccttt gaaatagtca ccagtgagga tctgcacatc
      841 ctcatcgacc cctttataac agagaatccc gcctgcacct gcagtttgga tgaggtccag
      901 gccgatgtga tctgtgtgac ccacggccac aacgatcact tcggagacac cctgaagctg
      961 gctgaaaggg atggatccct ggtgatctgc atccatgaac tctccaagta cctggagtgc
     1021 cagggaatcg aggcctgggg catgaacatg ggcggtaccg tggaggccag gggcgttaaa
     1081 gtaaccatgg taaactccaa ccactcctcg gatatggact tcctggaggg catgggcccc
     1141 ggggggagtt cctgtggctt catactggaa ctggaggatg ggaaaaagat ttaccactcg
     1201 ggagatactg gaatcttcgg agatatgaag actgtgatta gagatattta cagtccccac
     1261 ctggcaatgc tgcccattgg ggaccgtttc accatgggcc cccatgaggc taagatagca
     1321 gtggagtggc tggatgtgga tgtggtaata cccatgcact acaacacctt cccggtgata
     1381 gaacaggacc cagagaagtt caaagagtat gtggagtcca ctaccggggc aaaagtcgtt
     1441 gttctagagc cgggtgagac ttaccaggag tgattatgtg gttaatgttg ttttcaagat
     1501 tttcaggcga aactactttc gcgatatatt ctacaagcta attggaaaaa ataaaaaact
     1561 taagatcgga ttttacggac accccaactc agggaaaact acccttgcta acaggataac
     1621 cacggactgg atcggccagt ccctgggact ggtatccgag gtgccccacg agacccggcg
     1681 ggtttaccgc caggaacacg tgaccctcaa gcaggacggc atggagctgg acttcgacat
     1741 catagacacc cctgggatcg ccaccaagat cgactacaag gactttctgg agttcggcct
     1801 atccgaggag gaagccaagg aaagggctaa agaggcgact aaggggatca ttgaggccat
     1861 aaaatggttg gatgatgtga ctggtgtact gctggtggtg gacgccaccc aggacccact
     1921 gacccaggcc aacatcacca tcatgggtaa cctggaggcc cgcaagatac ccttcctcat
     1981 agtggccaac aagatagacc tacccgaggc ctccaccgag aggatcaagg cggttttccc
     2041 ccagcacagg gtggtatcca tctccgccct gcagggagat aatgtggaag gcctctacca
     2101 ggccatggtg aaccatttct aggagtgatt aatgtggaag aaataagtaa cagtctcaag
     2161 atggatttcc tctcatccga tgcccttaag aaccgctcca gtatggagaa aatcgccatg
     2221 atcgtggata aagtaaaaaa tggtgacctc ttggttatgg agggcggctt aacccccagt
     2281 gaggaagcgg agctcataga aaccactatg cgggagatcg atgtggaaaa cttcctggga
     2341 atcgacatct acaccctgga gaaggatcag agcacattct tcggcttgtc caagcgcaaa
     2401 accgtgggta taaccatcat tggcccggcc aatgtcatga aaaccgttaa aaggaagtcc
     2461 aacttcctgt ccatgattgc ccaactgggt ggtgccgatg caccggtgca ttaagtgcca
     2521 tgccgagtac gaatcatcag agatcctcct ggagggctgt ccagagtgtg gtagtaagta
     2581 ttttgaatac caccaggagg gaaaaccccc taagataagg gaagcccggg gggactctat
     2641 tgagaccatc atggtccatg agcatggggt ctacgaggtg gatctgccca gcctactggg
     2701 ggatgactcc atcattgtct ctgatgagga gggcaagtac ctcattgacc ttaacttcct
     2761 cttaaaaaag gggttgaagg agaaaaaaag gcattaaacc tactatttat tggacatcag
     2821 ctgccaggaa cccataccta tggctatgaa gggatcaaca ggtagctgat ccttacccag
     2881 aggtgctccg taaaaactca tctgcacggt aagagcaagg tcaatggtgg tgctgatgat
     2941 aaaccaggat catcccccca tcttttttag gattagaaaa gatcagcaca cacagtggtc
     3001 agggaaatag ccagggacgt gatggattct aaaagtggac tgtccatcaa cttcaggggg
     3061 attaaaatca gtactccatt acttcaggga gttttttagc aaattcaatg cacataacca
     3121 ggtcattttc cacaaatcgg gccttggtat gtttatggtt ctggttgata ttcactagtt
     3181 ctgcgtagaa ttcactggca tctgcctcgg ataccttctt cacactatcc attccggctt
     3241 ccaggaacat ccgggcgaag accggtccca cccactttat ccgggacaga tccgaaagtt
     3301 tggctaactc ccggatttcc tcccgcctca accccacctc ttcacccagc tcatccctat
     3361 cctggctggt tttcgccctc ttaaaaagtt ctagggtggt ttttatcccg gcagcctcga
     3421 gcttttctac aagttcaggc ttaactcctg ggagtttgga gagcttaact ggccctggtt
     3481 tcaggctcaa gacctccctt cttaaaacca gaaggtaatc ctctggtagc cctgtttgtg
     3541 aggcaaattc tcggaccttc ctggatgttt ttaaagcgtc catcagttcc tggaggttag
     3601 atatcccctt cctggatagg atgttaaacc gggcatccag gtcctccttc agtatctgcc
     3661 ggctgggaag aagggaggct ttctttagtt cctcttgcca ggtgtgtaat ggataattct
     3721 ggaagtctaa atagtaatca tcactcatac taggctctcc catcctacta tacaaggtgg
     3781 aggatttctt tggttccgca tccccacctg ggtgaatctg gaatggaaaa attcattttt
     3841 ttctgatctt gatccttaca atattcatgt taagggataa gtttttaaca aaatccatac
     3901 ctcagcctct tcatggatat tgtttcctga tactcgtctt tatatctaac taatgattct
     3961 tcacagctag gttaaaaagg tca
//
LOCUS       DAOQVW010000087         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25930_length_3937_cov_4.013395, whole genome shotgun sequence.
ACCESSION   DAOQVW010000087 DAOQVW010000000
VERSION     DAOQVW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25930_length_3937_cov_4.013395"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKK85_02785"
     CDS             <1..326
                     /locus_tag="PKK85_02785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNS24978.1"
                     /translation="SGYYDGLTFHRVIPNFVIQGGCPKGDGTGGPGYTIKCEINPHKH
                     GTGALSMAHAGKDTGGSQFFITHSPQPHLDGVHTVFGKVIKGMDVVNAINANDVMRKV
                     TVFDE"
     gene            568..846
                     /locus_tag="PKK85_02790"
     CDS             568..846
                     /locus_tag="PKK85_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24979.1"
                     /translation="MKKEAINYLESYGNIIIYVIFIMGTVMVANLFNAWSGNTPQVII
                     IIMCLLVLFDIFRQSLVDPKKSKMLRNIILLILMGLVIITIFWFFFLK"
     gene            complement(873..1424)
                     /locus_tag="PKK85_02795"
     CDS             complement(873..1424)
                     /locus_tag="PKK85_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNS24980.1"
                     /translation="MKKSMEKEKNRKFEVQEKLNYLKQQVEGLSPESSLKEVSDLFLD
                     VVTQLTSSLHGYMAYVDPENGDSVGISFSHLTSSCAMYEEMGEARFKVLEDGSYGGLL
                     GYSLDTGRSIYSNDPANHPAAHGLPPQHEPVERFLSVPVPDGKEVLGQIVLGNSEVDY
                     DESQLLIAVQVAEIYAQVLKRLL"
     gene            complement(1493..2107)
                     /locus_tag="PKK85_02800"
     CDS             complement(1493..2107)
                     /locus_tag="PKK85_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24981.1"
                     /translation="MGEFSKTLEDFQISAGRAYRKLMDSLEEDYCWKCPMHSTSKHAN
                     CKELHAWIKIQKALEEGVWEKIREERSDTELEVITARFLEKRFKKEKRRVSDALVLIK
                     VDDDLHPDVPISSALLIKPHPKKVKKGDLVLVPKELPCPVSWFYKAGLSVGVPFQITR
                     VSKVYHHGSVWYLKTVQNLDIPIPLVLGVILKVLDKESSIYNFI"
     gene            complement(2113..3096)
                     /locus_tag="PKK85_02805"
     CDS             complement(2113..3096)
                     /locus_tag="PKK85_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNS24982.1"
                     /translation="METELEINSAEFNGPLNIPLTIHSGQTSQPAWKEENSFFKELVS
                     IDGIPCLIEVAHHANTNTPVMVKATSPGDISIEEVIVNVKDIFDLNFDLNLFYEFLMD
                     DPLLAPTIDFCRGLRLFKAHDPFECIISSISSANCSIIRWTRSIQDMKCKWGQSYHFS
                     SGNFYTFPAPEILAHVPEHDLEEMQRQEEELPKDFLFQNNLQACGVGYRAKYIIRTAR
                     AVQDWLNLDELARMRYDDAFHTILDLPGVGPKVADCILLYGFGMGEAFPVDVWIKRIM
                     EHLYFPGEELKPHEVREFGRERYGKYAGYVQLYLFHYARKSGLLESLRPQK"
     gene            complement(3264..3755)
                     /locus_tag="PKK85_02810"
     CDS             complement(3264..3755)
                     /locus_tag="PKK85_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS24983.1"
                     /translation="MNRRKHIFLDEKTKIQRMENKIKKYEKYKPYHEKTAEDDFRLQE
                     KVAMLSIVASNFMMTGHSPTLVLTKREDGDEVMLPVGGGQKDRAREIISKIDFRKLYR
                     GGKGRPTDPLMIITAICMYVMRQDNPRRGDIRYSNKFIRKVGVNKEIYQHISQKLDSY
                     QKS"
BASE COUNT         1059 a          880 c          793 g         1205 t
ORIGIN      
        1 aatctggata ttatgatgga ttaacctttc acagggtaat acctaatttc gtaatccagg
       61 gaggatgtcc taagggtgat ggaactggag ggccaggtta tactataaaa tgtgaaataa
      121 atccacataa acacggcact ggagctttgt ccatggccca cgcaggtaaa gacaccggtg
      181 gcagccagtt cttcatcacc cacagtcccc aaccccacct ggatggcgtg cacaccgtat
      241 ttggaaaggt aataaagggg atggatgtgg ttaacgccat caatgcaaac gatgtgatgc
      301 gtaaagttac cgtgttcgat gaatagtaaa ccaaaggaat ggtggttaaa aaaaatcaag
      361 gatacacaaa tttctttttt tatttcgatt tcataggcac agtaatgatt cacaggccta
      421 tcacggggca ggatcacaaa agaacacctt gtgaatttac attaccacca taccttgctc
      481 ctgatagaat tcaattggaa ccaggattaa gtgaatactc cactatggaa aaatatacat
      541 ataggaatcg ggatataggg tagtgtaatg aaaaaggaag ccattaatta tctggaaagt
      601 tatggcaaca tcataatcta cgtgatattt ataatgggca ccgtgatggt ggctaatctt
      661 ttcaatgcct ggtccggtaa caccccccaa gtaataatca ttatcatgtg cttgttagtt
      721 ctatttgaca tatttcgcca gtccctggtg gatcctaaaa agagtaagat gctccggaat
      781 attatactcc tcatcttaat gggcctggta ataatcacca tcttttggtt ctttttttta
      841 aaataagtat tcattcaaga atatatagtg atctaaagca accttttaag aacctgggcg
      901 tatatttctg ctacttgtac tgcaatcagt aactgtgatt catcgtaatc cacttccgag
      961 tttcccagaa ctatctggcc cagtacttct ttcccatctg gaactggaac cgataggaac
     1021 cgttccactg gttcatgttg tggtggcaat ccatgggcag ctggatggtt agctggatca
     1081 ttggagtaaa tggatcgccc agtatccagg gaatatccca gtagaccgcc atagctacca
     1141 tcctccaaca ctttgaatct ggcctctccc atttcttcgt acattgcaca ggaacttgtt
     1201 aagtgtgaaa aagatattcc cacactatca ccattttctg ggtctacata ggccatatag
     1261 ccatggaggc tgctggttaa ctgggtaacc acgtccagga aaagatctga aacttccttt
     1321 aatgatgact caggacttaa accttcaact tgttgtttta gataattaag tttctcctga
     1381 acttcgaatt tcctattttt ttccttttcc atgctctttt tcatgataaa tcccccgggt
     1441 tattattttc aattcatcat ggtttttaat ttatacttcc agagatatta tgttatataa
     1501 aattatatat agagctctcc ttatccagca ccttcaaaat cacgcccaat accaagggaa
     1561 taggaatatc cagattttgc acagttttca agtaccacac tgagccgtgg tggtatactt
     1621 tactgacacg ggtgatttga aatggcaccc ccactgacaa accagcttta taaaaccatg
     1681 aaaccggaca gggcaattct tttggtacta agaccagatc acccttttta acctttttgg
     1741 ggtggggttt aataagcagt gcactgctta ttggaacatc ggggtgtagg tcatcatcaa
     1801 ctttgattag tacaagtgca tcacttaccc gtcttttttc ctttttaaac cttttttcca
     1861 ggaatctggc ggtgatgacc tctaactccg tgtcagaacg ttcttccctg attttttccc
     1921 aaaccccctc ttccagggct ttctgtatct tgatccatgc gtgcaactct ttacagttag
     1981 catgtttact ggtggagtgc atgggacact tccagcagta atcctcttcc aggctgtcca
     2041 ttaatttcct gtaagctctt ccagcagata tctgaaaatc ttccagggtc tttgaaaact
     2101 ctcccattgt aatcactttt ggggccgtaa tgactcaaga agcccggact tacgcgcgta
     2161 atgaaacagg tatagttgca cgtagccagc atactttcca tacctttccc ttccaaattc
     2221 acgtacttca tggggcttta attcctcccc cgggaagtaa agatgctcca tgatacgttt
     2281 gatccacacg tccactggga aagcttctcc catgccaaat ccatatagca gaatgcagtc
     2341 cgccacctta ggacccactc cgggaaggtc caggatggtg tggaaagcat catcatacct
     2401 cattctggct agttcatcca gattcaacca atcctgcact gcccgcgcag ttcggatgat
     2461 atatttagcc cggtaaccca ctccacaagc ttgtaggttg ttctggaata aaaaatcctt
     2521 tggtaattcc tcctcctgtc tctgcatttc ttccaagtca tgttcaggga catgggccag
     2581 aatttcaggt gcagggaagg tgtaaaagtt tccagaacta aaatggtaag attgtcccca
     2641 tttacacttc atatcctgga tggaacgggt ccagcgtatg atggaacagt tagctgagga
     2701 tatggatgaa ataatacatt caaatgggtc atgggctttg aaaagccgca gaccacgaca
     2761 gaagtcgatg gtgggtgcta gcagggggtc atccattaaa aattcataga agagatttaa
     2821 atcgaagttt aagtcgaata tatccttcac gttaactatt acctcttcaa tggagatatc
     2881 accaggagat gtcgccttca ccataaccgg ggtgttggtg ttggcgtggt gggccacttc
     2941 aatgaggcat ggaatgccat ctatagaaac tagttccttg aagaagctat tttcttcttt
     3001 ccaagcaggt tgagatgttt ggccgctgtg gatagttaat ggaatgttaa ggggaccgtt
     3061 aaattcggct gaatttattt ccaattcagt ttccatacat ttattcctag atgatcaagg
     3121 ggtataactg ctttggtgtc cctaacaaca cctactacta gggtcggggg atgggtttat
     3181 agcgctgcaa atgaaattgg aaatttctta gaaaaaaagt aggttttttt tatccatcac
     3241 tatatagagc cacatatcaa aacctaggat ttctggtaag aatccagttt ttgacttata
     3301 tgttgataaa tctccttgtt cacaccaact ttcctaataa atttgttgga atatcttata
     3361 tctccccgtc gtgggttatc ttgcctcata acgtacatac agatggcggt tatgatcatc
     3421 agggggtcgg ttggtctacc tttacctcca cggtagagtt ttctgaaatc gattttggaa
     3481 attatctccc gggcccgatc cttttggcct ccacccactg gtaacatgac ctcatcccca
     3541 tcttcccttt ttgttaaaac cagggtgggg ctgtgtcctg tcatcatgaa attgctggcc
     3601 actatgctca gcatggccac cttttcctgc aaacgaaaat catcctcagc tgttttttca
     3661 tgatatggct tgtatttctc gtattttttg attttatttt ccattcgttg gatctttgtc
     3721 ttttcgtcca ggaatatgtg ttttcttctg ttcaaatccc tcgcctgata actgtttttt
     3781 taatgtgtta ctagtttaaa ggtcatatta gtccagatcc acttcactat ccccgtatct
     3841 gagacgcttc tttttaaccc acacaaaacc ctggtcaggg gtgatgacag ccacatctat
     3901 ggggccgccc acaccaggca tatcccccgg gtcggcg
//
LOCUS       DAOQVW010000088         3930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_25984_length_3930_cov_3.398194, whole genome shotgun sequence.
ACCESSION   DAOQVW010000088 DAOQVW010000000
VERSION     DAOQVW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25984_length_3930_cov_3.398194"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PKK85_02815"
     CDS             <1..559
                     /locus_tag="PKK85_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNS24984.1"
                     /translation="LNEVEVKKLIHTIDESKDDSNHKKAFKHRNKLILTLLYSTGLRV
                     SELVTLETDSVDLDERTIRIRGKGEKDRIVLFDENTRLLLKDYLSKSSMEGDYLFVNR
                     SGNHLTPRYIQMMIKEYAEAAGIRKKVTPHILRHSFATHLLKNGVDIRAIQQLLGHSN
                     LSTTQIYTSVDMQTLKTVYDRAKLL"
     gene            788..1090
                     /locus_tag="PKK85_02820"
     CDS             788..1090
                     /locus_tag="PKK85_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNS24985.1"
                     /translation="MTVNRKFFLGFIRIHILYRAQNENIYGVQMMDELSNQGYKLSPG
                     TLYPILHSLEQDGFLKSQRMNVEGKIRKYYTTTPAGNKILGEALAKFKKFTEEVMG"
     gene            2069..2677
                     /locus_tag="PKK85_02825"
     CDS             2069..2677
                     /locus_tag="PKK85_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNS24986.1"
                     /translation="MEITVENYFRKRYSLYKWRSETSLINKLILAFFMACVTGIMAQL
                     VIPLPWTPVPVTAQTFAVLMAGVLLGRWWGGISQIIYLVAGLMGLNWFAGLSGGFSML
                     FSATGGYLVGFILVALFLGYFSDGYVGARKFRPMLGLMLFANFVFIYVPGLFGLGLWI
                     YLVKGSYPGIITLVSMGLLPFLVGDLIKIGGAAAITKMVTPN"
     gene            2713..3108
                     /locus_tag="PKK85_02830"
     CDS             2713..3108
                     /locus_tag="PKK85_02830"
                     /inference="COORDINATES: protein motif:HMM:NF018621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HNS24987.1"
                     /translation="MIPIKIRAHHLLCIQGYQGHGYSQDFEENMGRMVEKFRVNPTLP
                     VMVVCETDAICEYCPHQSAGRCTNTTLTHEQISKMDSLVIEKLNLVSGATIGINKLIS
                     LTKKLNKTDVVEICDGCSWRECCLWFQDK"
     gene            complement(3250..>3930)
                     /locus_tag="PKK85_02835"
     CDS             complement(3250..>3930)
                     /locus_tag="PKK85_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS24988.1"
                     /translation="FDPQDVYLLNRKGDNLSRLGLFQEAVQSYDDALKLDPENEFIWN
                     NRAIALLNYNQPQEALKSNDEAYKINPDNLLVLYWRGIILEMLGRLKEALEEYDRILE
                     LDPNHPEAWNARGNILSQMERTEEAIESYDRALELCLDENPDASTWNRKGNALLELDR
                     LEEAIKCYEEALKLDEYNDVFLSNLGVTYMELSRFQEAMETFKKALSINPHNEDARIL
                     MDECLENL"
BASE COUNT         1173 a          692 c          813 g         1252 t
ORIGIN      
        1 ccttaacgaa gtggaagtta aaaagcttat acataccatt gacgagagta aagatgattc
       61 aaatcataaa aaggcattta aacatagaaa taagcttata ttgacccttt tatactccac
      121 tggccttcgt gtttcggagc tggtaaccct agaaactgat agtgtggacc tggatgagcg
      181 taccattcgc ataaggggta aaggtgaaaa ggatcgtatt gttctttttg atgagaacac
      241 ccggctttta ctaaaagatt atctttctaa aagttccatg gaaggagatt acctttttgt
      301 caatcgatcc ggcaaccatc taactccccg ctatattcag atgatgatca aagaatacgc
      361 tgaggcagca gggatcagga agaaagtaac tcctcatatt ctgcgccact catttgcaac
      421 ccatctcctt aagaatgggg tggatataag ggctatccaa caacttttag gccattccaa
      481 cttgagcacc actcagatat acactagtgt ggatatgcag actctgaaaa cggtttatga
      541 ccgtgcaaag ctactttgat aagggacttt ttatcatagc cactaattaa gtaaaaagat
      601 tgaagttttt accaccctat atgaaataat catgaaaaag taatacctat ttttttttgt
      661 tgaacaattc tcattatcat ttaccgagaa atttctaaat ttttaaaaaa ttttataaaa
      721 acaaaaatca atattcgata ttgtaattag ttattgattc caaatatcga aaaacgatag
      781 tgattttatg actgtaaacc gtaaattctt cctgggattc attagaatcc atattttata
      841 ccgtgcacag aatgaaaata tttatggggt acagatgatg gatgaacttt caaaccaggg
      901 ttataaatta agtcctggta cattatatcc catcttacac tccctggaac aagatggttt
      961 cttaaaaagt cagaggatga atgtggaagg taaaataaga aaatattata ctactactcc
     1021 tgctggaaat aaaatactag gagaagcact ggcaaaattt aagaaattca ccgaagaagt
     1081 aatgggatag atccttactg tgattttttt taaatcctca actaaaaacc aaaggattac
     1141 aaagaacata ctggatttat atggctgcaa caaccattat ggtattgatt ttgccgattt
     1201 tctattcata gcattccatt tccagaaatc aagtattcaa tcatatatgc aggcatgggc
     1261 atggatgttc tggctacttc aacttttaga cgtttatttg atggtattgg ggtgttatca
     1321 ggggccttag ccactacgat catatcattc tatgctcttc tagtatttct gggaaaattt
     1381 gagcagtatt tggggaatgg tgttctgggg aataggaatg ggtgctacag gaatcggtaa
     1441 tgagggctcc aattgtagaa ttatcgccat taaaacgttg ggggtggctt atggtttttt
     1501 taacaccata ttacggcgtc cttgttttat ggaagcttaa taatggggtt tgtgcaatga
     1561 tcttaatctc atccatgaga cggttttatc cattagccca atttatggcc ataccattct
     1621 gattttgaca cagcccatga tttattgcgt aaataaacat tatttgctga tctattaatt
     1681 agataatcac tggttaaagt gatttgaaaa ggtaaagata aatatgggga gtggcctatg
     1741 ttttggcaaa tagactgatc ctggaaggtg gggagataat actggtaagg ttctttacat
     1801 tataaataag gacttgcact gtaaatttag aattgaccga taggtggagt taattgaaaa
     1861 tatagtaaga taaatacttc ttttttgtat tatttgtacc aaaattgtac ctagccctgt
     1921 aacaaaatat atatctataa attgtccaat aaaataaagg gtctaaaggc ttataacatt
     1981 tctaagatgg aattaaaatc aatgtaaacc cttatcaggg ttttttaagg tcaattttca
     2041 aataaactat aatcgcgggg aattcaaaat ggaaattacg gttgaaaact atttccgaaa
     2101 aagatattca ttatataagt ggcgttcaga aacttctctt attaataaac ttattttagc
     2161 atttttcatg gcttgtgtaa ctgggataat ggctcaatta gtaatacctc taccctggac
     2221 accggtacca gtgacagccc agacattcgc cgttttaatg gcaggggttc tcctaggtcg
     2281 ctggtggggg ggaattagtc agattatata tcttgtagca gggttaatgg gattaaattg
     2341 gtttgctgga ctaagtgggg gtttttccat gttgttcagt gcaaccgggg gctatttggt
     2401 tggatttata ttggttgctc tatttttagg ctacttctcc gatggatatg tgggggctcg
     2461 gaaattcaga cccatgctgg ggctgatgtt gtttgccaac tttgtcttca tctatgtacc
     2521 agggcttttt ggtctggggt tatggattta cttggttaag ggtagctatc ctggcattat
     2581 cactctagtt tccatgggac ttttaccgtt cttggtgggt gacctgatta aaataggtgg
     2641 agctgcagcc ataaccaaga tggtgactcc taactaagtt ttaggtgaaa tgaaataatt
     2701 gttgattcgg ccatgatccc catcaagatc cgagcccacc acctcctctg catccagggt
     2761 taccaaggac atggttacag ccaggacttt gaagaaaata tggggaggat ggttgagaag
     2821 tttcgcgtaa atccgactct tcccgtgatg gtggtatgcg aaacagatgc catatgtgaa
     2881 tactgtcctc atcaatctgc tggtagatgt accaacacta ccctgaccca tgagcaaatc
     2941 tccaaaatgg attctctggt gatagaaaag ctgaatctgg tgagtggagc tactattggc
     3001 attaataaac ttatatctct caccaaaaaa cttaataaaa ctgatgtggt ggagatctgt
     3061 gatggatgtt catggcgaga gtgctgttta tggttccagg ataaataact ttaagcaatc
     3121 attctactca aaacaactat atcagcgctt gatggttggg caataaaaat tttcaagagg
     3181 agactatcag atatggtact ttcacaattc gatataaggg gagtttaatg gaaagtatgg
     3241 tccctttgtt tacaaattct ccaaacactc gtccatcagg atccgggcat cttcattgtg
     3301 gggattgatg cttaatgctt ttttgaaggt ttccatagcc tcctgaaatc tggagagctc
     3361 catgtaagtt actcccagat tactgaggaa aacatcgttg tattcatcca actttaaagc
     3421 ttcttcataa cattttatgg cttcttctag ccgatctaat tctaaaaggg cattgccttt
     3481 acgattccat gtggatgcat cgggattttc atctaaacat aattccagtg ctcgatcata
     3541 ggattctata gcctcttcag ttctttccat ctgggatagg atgttacccc tggcattcca
     3601 ggcttctgga tggttggggt ctaattctaa gattcgatca tactcttcca gagcttcttt
     3661 taaccgcccc aacatctcca gtatgatacc tctccagtaa agtacaagaa gattatcggg
     3721 gttaattttg taggcttcat cattagattt aagtgcttct tggggttggt tgtagttcaa
     3781 aagcgcaatg gcccggttgt tccatatgaa ttcattttct ggatccagtt tcagggcatc
     3841 atcataggac tgcactgctt cctggaataa tcccaaccgg gagaggttat ccccttttcg
     3901 gttaaggagg tatacatctt gggggtcaaa
//
LOCUS       DAOQVW010000089         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_26129_length_3913_cov_3.739502, whole genome shotgun sequence.
ACCESSION   DAOQVW010000089 DAOQVW010000000
VERSION     DAOQVW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26129_length_3913_cov_3.739502"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKK85_02840"
     CDS             complement(<1..371)
                     /locus_tag="PKK85_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNS24989.1"
                     /translation="MDNIIQKVKIIKVGQNSQIVEDRVAIDAKIELEVNGGKIGEFYL
                     SPMDLEDFTFGYLRDGLYIKSKDEVENIEVDKSVIKVTLSPEKTVVEGNIGCYDGWRD
                     REQEITRVESDFKVDRKEIFDS"
     gene            complement(406..720)
                     /locus_tag="PKK85_02845"
     CDS             complement(406..720)
                     /locus_tag="PKK85_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS24990.1"
                     /translation="MARELPKHYQNIRKRYEEYGQALSELGKAMKDAGPLDEKTSQLI
                     QLGASAAIRSEGAVHSHTRRALEEGASADEIYHSVLILTSVIGFPNVAAAVSWIDDVV
                     QG"
     gene            complement(955..1881)
                     /gene="moaA"
                     /locus_tag="PKK85_02850"
     CDS             complement(955..1881)
                     /gene="moaA"
                     /locus_tag="PKK85_02850"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNS24991.1"
                     /translation="MVKIDKFERPIISLRISITNRCNVKCFYCHHDGIIPQKYEMTPE
                     EIMRISQVAKNIGIIKIRLSGGEPLIRKDIIDIVNSISKIGFTDVSITTNGILLEEYA
                     QPLKDAGLDRINVSFDTLNPETYRFITKSDYVNQVKRGIEKAVEVGLYPVKVNMVVMR
                     GLNHEEIWDMFHFCKETGAVLQLIELLKTENCPDEDFFDDYYYDMSIIEKKLLEKADN
                     VKTRRFMQDRKKYYLEGGEIEVVKPMDNTEFCKNCTRLRITPEGKLKPCLLRNDNLVD
                     LMEPIRRGVSDEYLESLFVEAITNREPYYAGC"
     gene            complement(1874..2578)
                     /gene="mobB"
                     /locus_tag="PKK85_02855"
     CDS             complement(1874..2578)
                     /gene="mobB"
                     /locus_tag="PKK85_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNS24992.1"
                     /translation="MKILGVAGTKDTGKTTLVTRIVQELTNRDYKVATIKHTHHDFDL
                     EGKDTWKHREAGAELVVGSGLNTFFLVKDQIELDRIIYIIEKITGPDYIVIEGLKHSN
                     YSKISTTSKLKDDFTICNVNVFEMGKKDIRTIVDMIEKRSYGTIPYSDCGECGFENCL
                     EMAGAIVKGEASEEECKMRKFKEVELFIREERIPLSPFVQDILKSTVMGMVKTLKIED
                     DMDINKENVELFIRNG"
     assembly_gap    2603..2612
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2638..3852)
                     /locus_tag="PKK85_02860"
     CDS             complement(2638..3852)
                     /locus_tag="PKK85_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNS24993.1"
                     /translation="MSLNSLISVKKATERIESLKFPLKSQNIPLETAYGRISAENICA
                     LLDAPPFDRAAMDGYALRSMDTVQSSPKNPLYLDVVDDIGAGESSNLKLNQNQAVRIS
                     TGAPIPPGADAVIMKEKVEESDFQIKVTEGVTIHQDVALKGEDFHRGDALLSEGQLLN
                     PNQIALVASAGINQVKVVKKPAIGIINTGNELIEPTINPKPNMIINSNKYALIGLVGE
                     CLARSYQYLCKDDLEDLKKQLKQAAKSYDAVITTGGTAISKGDVVLEAVQELGEVLFH
                     GVSIKPGKPLGFGIVDGKPVFMLSGYPVAAAVQFDFFVRPSLFRMQGIDFRMKFQEFE
                     VGSEVKSAKDKFTVMRACLDDEIVYPLRTKAGINKSITNSNCYLILDEDTGKLNKGEM
                     CPVIKYEDLRIC"
BASE COUNT         1064 a          869 c          652 g         1318 t
ORIGIN      
        1 gaatcaaaaa tctcctttct atctacttta aaatcagatt caaccctggt gatttcctgt
       61 tcacggtctc tccatccatc atagcatccg atgttacctt caaccaccgt tttttccggg
      121 cttaatgtga ctttaatcac tgatttatct acttctatat tttcaacttc gtctttggat
      181 ttaatgtata atccatcccg cagataacca aaggtgaagt cttccaaatc catgggactg
      241 agataaaact ctccgatctt tcctccattg acttctaatt ctattttggc atctatagcc
      301 acccggtctt caacaatttg ggagttctga ccgactttta ttatttttac tttctgaata
      361 atgttatcca tttaacaccc cgattaaaaa aaagaaatgg aaaaattagc cctgcaccac
      421 gtcgtcaatc caggaaactg ctgccgctac attggggaaa ccaatgacac tggtcaaaat
      481 caatacactg tggtagattt catcggcaga tgctccttct tccagtgctc ttctggtgtg
      541 actgtgcact gcaccttcag atcttatggc agcagaggct ccgagttgaa ttaattgtga
      601 tgttttttca tctaatgggc cggcatcttt catagctttt cctaattcac ttaaagcctg
      661 accatattct tcgtaacgct tcctgatatt ctgataatgt tttggtaatt ctctggccat
      721 gatttacact ccacacatgt taactgatca tgtgatatgg ttgaagggat attaataata
      781 tgaaccttac ttttatccca aagctgtaac cagttctggt ctaattacct tctattctta
      841 ggttcaccaa gcaatatcta cagaaaaaat tcttaaaacc ctttaataat tcgacgtaac
      901 tggaggtagg ttacttataa tttaaaagaa aaccggcctt tatgttctaa agatttaaca
      961 gccagcgtaa tatggttctc tgttggtgat ggcttctaca aataggcttt ccaagtattc
     1021 atcagataca ccccttcgaa tgggttccat caaatccacc aagttatcat tacgaagtaa
     1081 acaaggtttt aattttcctt cgggggtgat cctgagtctg gtacaatttt tgcagaattc
     1141 ggtgttatcc atgggcttca ccacttcaat ttctccgcct tcaaggtaat acttcttcct
     1201 atcctgcata aaacggcgag ttttcacgtt atcagccttt tctaaaagtt ttttctcgat
     1261 gatgctcata tcatagtagt agtcatcaaa aaaatcttca tcagggcagt tttctgtttt
     1321 aaggagttct ataagttgca ggactgctcc agtttccttg caaaagtgga acatatccca
     1381 tatctcctca tggttgagac cccgcattac caccatgttc accttcactg gataaagacc
     1441 cacctcaact gccttttcaa ttcccctttt cacctggtta acataatcac ttttagttat
     1501 aaaacgataa gtttctggat ttaaagtatc aaaactcacg ttaatcctat caagtccagc
     1561 atctttcaat ggctgggcat actcttcgag gagaatgccg ttggtggtaa ttgaaacgtc
     1621 agtaaagcct atcttggata tgctgttaac gatgtcaatg atatcttttc ttatgagtgg
     1681 ttctccacca gaaagcctta tttttattat accaatgttt ttggccacct gagaaattct
     1741 cataatctct tctggagtca tctcatactt ctgggggatg atgccgtcat ggtgacagta
     1801 aaaacattta acattacagc gattggtaat ggagattcgg agggatatga tgggcctctc
     1861 gaatttatcg attttaacca tttcttataa acaactccac attctcttta ttgatatcca
     1921 tatcatcttc aatcttcaaa gttttaacca tacccataac tgtgctttta agaatatcct
     1981 ggacaaaagg actcaacgga atccgttcct ctctaatgaa gagttccact tccttgaact
     2041 ttctcatttt acactcttct tcggaagctt ctcctttcac aatagctcct gccatttcta
     2101 agcagttttc aaatccacat tctccacaat cagagtatgg tatggtcccg tagctgcgct
     2161 tttctatcat atccacaatg gttcgtatat ctttcttccc catttcaaac acgttaacat
     2221 tgcatatggt gaaatcatcc tttaacttag atgtagtaga aatttttgaa taattagaat
     2281 gctttaatcc ttcaatcacg atgtagtcag gtccagttat tttttcgatg atgtagatga
     2341 tcctatccag ttcaatctgg tctttaacga ggaagaaggt gttcaaacct gatccgacca
     2401 ctagctctgc ccctgcttca cggtgcttcc aagtgtcttt gccctctaag tcaaagtcat
     2461 ggtgggtgtg tttgatggtt gccactttgt aatcacggtt agttaattct tggactattc
     2521 tagtaaccag ggtggtttta cccgtgtctt ttgtcccggc aacacctaat attttcatat
     2581 tcagttcctc ctacaaacat tannnnnnnn nnatatagtt agcttattag aactttttca
     2641 acaaatcctt aggtcttcat attttataac tggacacatt tcacctttat ttaatttacc
     2701 ggtgtcttca tccaagataa gataacaatt ggaattggtt attgatttat ttatacctgc
     2761 cttggttctt agaggataca cgatttcatc atcaagacag gcacgcatca ctgtgaattt
     2821 gtcctttgca gatttaacct cagaaccgac ttcaaattct tgaaacttca ttctgaagtc
     2881 aattccctgc attctaaata gtgaaggtct aacaaaaaaa tcaaactgca ctgccgctgc
     2941 tacggggtac cctgacaaca taaaaactgg ttttccatct acaataccaa agcccagtgg
     3001 ttttcctggt ttaattgaaa caccatggaa taaaacctcg ccaagttctt gaacagcctc
     3061 caatactaca tcacccttgc ttatggcagt tcctcctgtg gttataactg catcataact
     3121 ttttgcagct tgttttagct gctttttcaa gtcttccaag tcatctttac acaggtactg
     3181 ataagatctg gctaaacatt ctccaacaag ccctatcagg gcgtacttat tagaattgat
     3241 gatcatgttg ggctttggat taattgtagg ttctattaac tcatttccag tatttataat
     3301 cccgattgca ggttttttca ccaccttcac ttggtttata cctgccgaag caacaagggc
     3361 tatttgattc gggtttaata attgtccttc actcaaaagt gcgtctcccc tatggaagtc
     3421 ttcacccttt agggcaacat cttggtggat ggttacccct tctgttactt taatctggaa
     3481 atctgattct tcaaccttct ccttcataat cactgcatca gcaccaggag gaatcggagc
     3541 cccggtagat attctcacag cttgattttg atttaactta agattggaac tttcaccagc
     3601 accgatatca tctacaacat ccagatacaa tggatttttt ggtgaagatt gaactgtgtc
     3661 catggatcgc agagcgtagc catccatggc agccctatca aatggtggcg catccaaaag
     3721 ggcacagata ttctcagcac taattcgccc ataagcagtt tctagaggaa tattttggga
     3781 ttttaaggga aatttcagac tttcaattct ttctgtagct tttttaacgc tgatcaaact
     3841 gttcaatgac attaaagcac cctgaacttt gataaccatg ttttttagtg aatgtggata
     3901 gtaaggttat tcg
//
LOCUS       DAOQVW010000090         3910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_26164_length_3910_cov_3.444358, whole genome shotgun sequence.
ACCESSION   DAOQVW010000090 DAOQVW010000000
VERSION     DAOQVW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26164_length_3910_cov_3.444358"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2928)
                     /gene="carB"
                     /locus_tag="PKK85_02865"
     CDS             complement(<1..2928)
                     /gene="carB"
                     /locus_tag="PKK85_02865"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNS24994.1"
                     /translation="MPRDKDIKKVLIIGSGPIQIGQAAEFDYSGSQACKSIQEEGIET
                     ILVNSNPATIQTDINMADQVYVEPLTPEIVAKIIEKEKPDAVLPTMGGQTGLNVATGL
                     EEIGALEGIKVIGSSVETIRNVEDRDLFDSFMKNLKEPVPRARAVESLEDAIKAVEEI
                     GYPVIVRPAFTLGGTGGGVAHNEEELREVATRGLDMSLINQVLIDESVIGWKEFEYEV
                     MRDKNDTCIIVCNMENIDPMGIHTGESIVLAPSQTLSDVDNQKLRNASIKIIRALQIQ
                     GGCNIQFAVHPETGEYKVIEVNPRVSRSSALASKATGYPIAKISAKIAIGMTLDEIDN
                     DITKETPASFEPTLDYVVAKIPRWPFDKFKGISKEIGVQMKSTGEVMSIGRTIEESLH
                     KAIRSLDISRYGFEEVPFTRDDLANPTDERIFQVYTALKQGMDLEEIHSITRIDKFFL
                     HKIMNIIEFEEKINPESLQDPIIMAESKRMGFSDRTLAQIAGVDEEEIRRHRRDMGIN
                     PSYKMVDNCAAEFEAVTPYYYSTYEFEDEIAVSNDKKVIIIGAGPIRIGQGIEFDYCC
                     VHSALALKEEGIETIIVNNNPETVSTDYDISDKLYFEPLTLEDVMAIVEKEQPHGVVV
                     QFGGQTSINLAVPLANNGVKILGTPHESIDRVEDRERFTEVLNQLEIPQADYGIAYSF
                     KDAQDVAQRIGYPVLVRPSYVLGGRAMEIVYDDLELQEYMKEAVRISPDHPILVDKFL
                     EDAIEIDVDALCDGEKVFIGGIMEHIEEAGVHSGDSACVIPPQSISTDVLETIKNYTH
                     KLALELEVVGLMNIQYAVKMEEDHSPTVYILEANPRASRTVPFVSKAVGVPLAKIAAK
                     LMIGHKLLDFGLEDEIVVEHVSVKESIFPFIKLPEADSVLGPEMKSTGESMGIDENFG
                     VAYYKALLSAGMELPHKGKIFISVRDSDKNKIRDIVEKAQDLGFDLMATRGTAKAVDG
                     LGDI"
     gene            complement(2997..>3910)
                     /gene="carA"
                     /locus_tag="PKK85_02870"
     CDS             complement(2997..>3910)
                     /gene="carA"
                     /locus_tag="PKK85_02870"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNS24995.1"
                     /translation="PLQGNCGISSEWFQSDGIKVEGYVVRDENLHPSHSLSEKSLSDF
                     LEEYQIPGISGVDTRSLTLKLRRFGTLKGALATAEIEDEELLDMARSQPGIEEIDLVD
                     EVCVDKPRILGEDYSYNVVILDCGIKNNIINALLKREIGVTILPYHTRPKEIMDYQPH
                     AMLVSSGPGDPTRVKEAIQTVKDLKEKMPIFGICMGQQIISRAFGAKIYKMKFGHRGI
                     NQPVKDLETGKVFITSQNHGFTVDPESLSDLPLKLTQINLNDDTPEGIKHEELPIESV
                     QYHPEAGPGPHDSDSFFDHFVEILKDY"
BASE COUNT          938 a          964 c          814 g         1194 t
ORIGIN      
        1 tatgtctccc aggccgtcta cggctttagc tgtaccccgg gtagccatca gatcaaaacc
       61 cagatcctgg gccttttcaa caatatctcg gatcttgttc ttatcagaat ccctgacact
      121 gatgaatatt ttgcctttgt ggggaagttc catccctgcc gagagcaggg ccttgtagta
      181 ggccacacca aagttttcat caatgcccat actttctcca gtggacttca tctccggacc
      241 caaaactgaa tcagcctctg gtaattttat gaaggggaat atggattcct taacagacac
      301 atgttccacc acaatttcat cttccagtcc aaaatccaac agtttatgtc ctatcattaa
      361 tttagcagca atttttgcca gaggaacacc aacagcttta ctaacgaagg gaacagtacg
      421 actggcccgg ggatttgctt ccaggatata aactgtgggg ctatgatctt cctccatttt
      481 cacggcgtac tgaatattca tcaatcccac tacttccaat tccagggcca atttgtgggt
      541 gtaattctta atagtttcta gaacatcggt tgaaatactt tggggcggta ttacgcaggc
      601 agaatctccg gaatgcactc ctgcttcttc tatgtgttcc atgatccccc cgataaacac
      661 cttttcacca tcacagaggg cgtccacatc tatttcaatg gcatcctcta ggaacttgtc
      721 caccagtatg ggatgatcag gcgagattcg cacagcctct ttcatgtatt cctgaagttc
      781 cagatcatcg taaacgattt ccatggccct acctcccaga acatatgaag gacgtaccaa
      841 aaccgggtat ccaattctct gagccacatc ctgcgcatcc ttaaaggagt atgctatgcc
      901 ataatctgcc tgaggtattt ccaattgatt aagaacttcg gtaaatcttt cccggtcttc
      961 aacccggtct atactctcat ggggtgttcc taaaatctta acaccattat tagccagggg
     1021 aactgccaag ttgatgctgg tctggcctcc gaactgcacc actactccgt ggggctgctc
     1081 tttctccaca atagccataa catcctccag ggtaaggggc tcgaaataga gtttgtcgga
     1141 gatgtcgtag tcggtactca cggtttctgg attgttgttg acaattatgg tctctatacc
     1201 ttcctctttc agggcaagtg ctgaatgcac acagcagtaa tcaaattcaa taccttgacc
     1261 aatccttatg ggtccggcac caataattat aaccttttta tcattagaaa cagctatttc
     1321 atcttcaaac tcataagtac tgtagtaata gggagttact gcttcgaact ctgctgcaca
     1381 gttatccacc atcttgtagg agggatttat tcccatatcc cttcggtgcc ggcgtatttc
     1441 ttcttcatct acccctgcta tctgggccaa ggtccggtct gagaatccca tacgtttgga
     1501 ttcagccatt ataatggggt cctgcagaga ttccggattg atcttttctt cgaattctat
     1561 gatgttcatt attttatgga gaaagaactt atcgatcctg gtgatgctgt ggatttcctc
     1621 cagatccata ccctgtttca gagcggtgta cacctggaat atgcgttcat cagtggggtt
     1681 tgccaagtca tccctggtga agggaacctc ttcaaatcca tacctgctga tatccaggga
     1741 acgaatggct ttatgcaaag attcttcaat ggtgcgccct atggacatga cctctccagt
     1801 ggacttcatc tgcactccaa tctccttact gattccttta aacttatcaa atggccatct
     1861 tggaatttta gccaccacgt agtcaagggt gggttcaaaa gatgcggggg tttccttggt
     1921 aatatcgttg tctatttcgt ccagggtcat gccaatggct atcttagctg agatcttggc
     1981 aattggatat ccagtggcct tggatgccag ggcactgcta cggctcaccc gtggatttac
     2041 ctcgattacc ttgtactctc cggtctcagg gtgcactgca aactgtatat tgcagccgcc
     2101 ctgaatctgc agggctcgta tgatcttgat ggatgcgtta cgcagcttct gattgtccac
     2161 gtcactgaga gtctgggagg gggccagcac gatactttca ccagtatgga ttcccatggg
     2221 gtcaatgttt tccatgttac atactatgat gcaagtgtcg tttttatccc gcataacctc
     2281 atattcaaac tctttccacc caattaccga ttcatcaatt agaacctggt taattaaact
     2341 catgtccagc ccccgggtgg ccacttcgcg aagttcttcc tcattatggg ccacacctcc
     2401 acctgtaccc cccagggtga aggccggccg gacaataacc ggataaccta tctcttccac
     2461 ggcctttatg gcatcttcca gtgattctac tgctctggct ctgggcacag gttcttttaa
     2521 atttttcatg aaactgtcaa acaggtcccg gtcttccacg ttgcgtatag tttccactga
     2581 ggatcctatg accttaattc cctccagggc gccgatctct tccagaccag tggctacgtt
     2641 aagaccagtc tgaccaccca ttgtggggag cacggcatcg ggtttttcct tttctatgat
     2701 cttggctact atttcggggg taaggggttc cacatagacc tggtccgcca tgtttatatc
     2761 ggtttggatg gtggccgggt tactgttgac cagaatagtc tctatcccct cttcctgtat
     2821 ggatttacag gcttgtgaac ctgaatagtc aaattcggcg gcttggccta tttgaattgg
     2881 tcctgatccg ataataagta cttttttgat gtctttatcc cgtggcattt acgatcctca
     2941 ttttataatg tttgttgaag gtattcaggg ttaattagat ttcaacagtt atctatttaa
     3001 taatccttta aaatctccac aaagtggtca aagaagctat cactatcgtg tggtcctggt
     3061 cctgcttctg gatggtactg cacgctttca atggggagtt cttcatgttt gatcccttct
     3121 ggagtgtcat cattcaagtt tatctgggtt aacttgaggg gaagatctga taaagattca
     3181 ggatccacag tgaaaccatg attctgggag gttatgaata ctttacctgt ttccagatcc
     3241 ttaactggtt ggtttatacc acgatgcccg aatttcatct tgtatatttt agcaccaaat
     3301 gcccgagata ttatttgctg tcccatgcag ataccaaata tgggcatctt ttccttcaga
     3361 tctttaacgg tttgaattgc ttctttaacc cgggtaggat ctccagggcc actcgaaacc
     3421 agcatagcat ggggttggta gtccattatc tccttgggac gggtgtggta gggtaaaatg
     3481 gtcaccccga tctcccgttt taaaagagcg ttgataatgt tgttttttat cccacaatct
     3541 aatattacca cattgtagga atagtcctct cctaaaatac gaggcttatc tacgcaaacc
     3601 tcatctacca ggtcaatttc ttcgattcca ggctgactac gggccatgtc cagaagttct
     3661 tcatcctcaa tttcggcagt ggccagcgcc cctttcaacg taccaaaacg acgtaatttc
     3721 agagttaagg agcgggtatc cactccactt atacctggaa tttggtattc ttctaaaaaa
     3781 tctgataaac tcttttctga tagactgtgg gatgggtgta ggttttcatc acgcaccaca
     3841 tagccttcaa cctttatacc atctgactgg aaccattctg atgatatgcc acaatttccc
     3901 tgcagaggat
//
LOCUS       DAOQVW010000091         3892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_26316_length_3892_cov_3.579880, whole genome shotgun sequence.
ACCESSION   DAOQVW010000091 DAOQVW010000000
VERSION     DAOQVW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26316_length_3892_cov_3.579880"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PKK85_02875"
     CDS             <1..697
                     /locus_tag="PKK85_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNS24996.1"
                     /translation="MDLFTHFLVPFIILNLLKVKNKFSGAFGGISIDFDFFLIGIGFI
                     APELFIFSHRGITHSFIFGFVTVVIFIYILTRPAVKSFINKLTKRPFNVEFNAESVSF
                     AYFGVIVHLFLDYLTTGGIPLFYPISLTRFSANIYYYTDVFTTLLAIAVIIILYLRLQ
                     HKYKKMAMFSFLMIISLWVELEPTKKLTPSRVRTSVMVTIPSPPTPPTIYLLGKWLKA
                     MVALNIAIIHITI"
     gene            complement(935..1861)
                     /locus_tag="PKK85_02880"
     CDS             complement(935..1861)
                     /locus_tag="PKK85_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNS24997.1"
                     /translation="MQSFGEKNYSNTIEKGQRESYDGFLNISKNILSFLIYNSLFISL
                     VCVFLTYVSFEVLNVQPNTILLFAVFLSTFSVYSLNRLTDLEEDSVNVPGRESYVKGK
                     EKSLLALCIISYGVALLLGFLVNPVTVLVLLFPLGIGILYSLEVSPKLPRLKNIPGMK
                     NFSVTLSWAAGAAFIPVVFSYPGLWITFLLFYFVFVKFLVNTISFDVRDIEGDKKSGV
                     RTIPVLLGRSRTKLLLFGIQSTLIFWMLIVISEGIFTRYWLLFTLSIVYGFWYIRYFS
                     ENRKRGTFSRDLLIDGEWMILAFLCFIISTFS"
     gene            complement(1797..2615)
                     /locus_tag="PKK85_02885"
     CDS             complement(1797..2615)
                     /locus_tag="PKK85_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNS24998.1"
                     /translation="MKYNAYELLDEVQDNLKFLAISGVRAKILITLQEGPKNLSELRE
                     LINLRSSTILHGMNELEKRNIVRKTGDRYNLSATGQILTLNFVDLVKSIIVAKRYEDL
                     WEGHQIDGIPVHLMLQIGSLINADLITSEPTDIYKPHLNFTQIASDSKKFWGISPFFH
                     SEYTELMIEMVSRGVDTKIIVTESIMNKLHELAAQDPEAFANLTSQENFELWITDKEV
                     KLGFTVTDRFISLGLALDDGTYDYSMDLVSDDHDAIIWGEKLFEYYRERSKRVI"
     gene            complement(2877..3494)
                     /locus_tag="PKK85_02890"
     CDS             complement(2877..3494)
                     /locus_tag="PKK85_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS24999.1"
                     /translation="MEKINDILMIEGRGYDSNIYVFDNVVVDTGTGENIGYFFDSIKN
                     AVINLEDLSLIVNTHNHYDHVGGNRYLDLPVAMHTDDAIPYEKGNDLATAASMFGKTM
                     GQMCVNHKLKEGDKIGRFEVLHTPGHTKGSICLFDGETLISGDTVFANGGFGRIDLGG
                     NLGDMVNSLERLSNLKVENLLPGHGPWTDHGSEHILWAKDLLSIY"
     gene            complement(3532..3684)
                     /locus_tag="PKK85_02895"
     tRNA            complement(join(3532..3566,3646..3684))
                     /locus_tag="PKK85_02895"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3647..3649),aa:Trp,seq:cca)
     gene            complement(3701..3772)
                     /locus_tag="PKK85_02900"
     tRNA            complement(3701..3772)
                     /locus_tag="PKK85_02900"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3738..3740),aa:Cys,seq:gca)
BASE COUNT         1178 a          801 c          699 g         1214 t
ORIGIN      
        1 tatggatctt ttcacccatt ttttagttcc tttcatcatt ttaaatctac taaaagttaa
       61 aaacaaattt tccggggcat ttggaggtat ttcaattgat tttgacttct tccttattgg
      121 cataggattt atagcccctg agttattcat attcagccac agagggataa cacatagctt
      181 catatttgga tttgtaactg ttgttatctt tatctatatt ttaacccgtc cagcagtgaa
      241 atcttttata aacaagttaa ctaaaaggcc ttttaatgta gaattcaatg cagaaagtgt
      301 aagctttgcc tactttggag ttatagtcca tcttttcctt gattatctaa ccaccggagg
      361 aataccacta ttttatccca tttcccttac ccgtttctca gccaacatat actactacac
      421 agatgtattt accaccctac tggccattgc agtgatcatt atactttatc ttcgtttgca
      481 gcacaaatac aagaaaatgg ccatgttcag cttcttgatg attatatctc tctgggtgga
      541 attagagcct acgaaaaaat tgacaccatc cagagtcaga acctcagtga tggttaccat
      601 accatcaccg cctaccccac caacgattta tttacttgga aagtggttga aagcgatggt
      661 ggcactaaat atcgctatta tacatataac aatttaaaaa aggaaaattc cactgaaatt
      721 agagaagtgt cgtgcctaac cattcaaaat ggaccctatc aagaggccca tgatgctata
      781 aagtatgctg ataacttgcc tgatgtaaga aaatttaagt ggagttcaca ttacaccata
      841 ataaatgcaa agcaggagtc cgggaaatgg gttataacct attcagattt tttaggatcc
      901 agggggcagg attgcatcac ggtaaatgtg ttcatcagct gaaagtggat attataaagc
      961 ataaaaacgc cagtatcatc cattctccat cgataagtaa gtctctggaa aaagttcccc
     1021 tctttctgtt ttcagaaaag tagcggatgt accaaaatcc gtatactatg gatagtgtaa
     1081 atagtaacca gtaacgggtg aagatacctt ctgaaattac gatcaacatc caaaatatta
     1141 gtgtggattg gattccgaat aacaggagtt tagtccttga tctccccaga aggacgggta
     1201 tggttctcac tccacttttt ttatcccctt cgatgtctct gacatcaaaa gatatggtgt
     1261 tcaccagaaa tttaacgaag acaaaataga acaacaaaaa tgttatccat aatccaggat
     1321 aactgaaaac taccgggata aaggcagctc ccgccgccca actcagggtt acactgaagt
     1381 ttttcatacc gggtatgttt tttaggcgtg gaagtttagg agacacctcc aaactgtata
     1441 atatgccgat gcccagtggg aaaaggagta ccaagacagt taccggatta accagaaaac
     1501 ctaaaagtag tgcaacgccg taggatatga tgcacaaggc caggagagat ttttctttac
     1561 ccttaacata agactcccgt ccgggtacgt ttactgaatc ttcttcgagg tctgtcagcc
     1621 tgtttaaact ataaaccgaa aatgtggata agaaaacagc gaaaagaagt atggtattgg
     1681 gttgcacatt taaaacttca aaggatacgt aggttaaaaa aacacaaacc agagaaataa
     1741 aaagggaatt gtaaattaaa aaagatagta tattttttga aatatttaaa aaaccgtcat
     1801 atgactctct ttgacctttc tcgatagtat tcgaataatt tttctcccca aatgattgca
     1861 tcatggtcat cactcactaa atccatacta taatcgtagg taccatcgtc cagtgccagg
     1921 cctaatgata tgaatctatc agtgactgtg aagcccagtt taacttcctt gtctgttatc
     1981 caaagttcga agttttcttg ggaagttaaa tttgcgaatg cttcaggatc ctgtgctgca
     2041 agttcgtgaa gtttgttcat tatgctttcg gttacaataa tcttggtatc caccccccta
     2101 gacaccattt caatcatgag ctctgtgtat tctgaatgga aaaatggaga aattccccag
     2161 aatttttttg aatcactcgc tatctgggtg aagttcagat gtggtttgta gatatcagtt
     2221 ggttctgacg ttataagatc cgcatttatc aaagatccta tttgcagcat caaatgaacc
     2281 ggtatgccat ctatttgatg tccttcccat agatcttcat agcgtttggc tacaataatt
     2341 gattttacga gatcaacgaa atttagggtg agaatttggc cagtggcgga aaggttgtaa
     2401 cgatccccgg ttttacgtac aatgtttcgt ttttctagct cattcattcc atgtagaatc
     2461 gttgatgatc gtagattgat tagttctcta agttcagata gattttttgg cccttcctgg
     2521 agagtaatca gtatttttgc tcttactccc gaaattgcca ggaatttcag gttgtcttgc
     2581 acttcatcca ataattcata ggcgttatat ttcattttgt ttccatccct taatttatta
     2641 actccaaaaa atattgctct taacgaacaa aactgtttaa gaaatccaat tttataaaca
     2701 ggaccttaaa tgcttggaat atgctgtgat aaccattgcc acaaatttac cctttcagat
     2761 gcacttttca ttcccccact agatttactc atgctagggt ctatcccctc actagcaata
     2821 gtttgcacta ctttaatata ttttcgtgtg atctatgtca gaaaactagt caataattag
     2881 tagatactca ataaatcctt ggcccataga atgtgttctg aaccatgatc agtccatgga
     2941 ccatgaccag gaagtaaatt ctctactttc agattactca atctttctaa agaattaacc
     3001 atgtctccca gatttccacc caaatcaatt cttccaaatc ccccattggc aaatactgta
     3061 tcaccagata tcagggtttc accatcaaaa agacatatac ttcccttggt atgtccaggt
     3121 gtgtgtaaaa cttcaaatct acctatcttg tccccttctt tgagtttatg gttcacacac
     3181 atctggccca ttgtttttcc gaacatactg gccgcggtag ctaggtcatt gcccttctcg
     3241 taggggattg catcatctgt atgcatggcc accggcaagt ccaggtagcg gttaccacct
     3301 acatgatcat agtggttgtg ggtgttcact ataagggaca ggtcttccag gtttatgact
     3361 gcatttttta tagaatcaaa aaaataacct atattttcac cggtaccggt gtccacaacc
     3421 acattatcaa atacgtagat gttagaatcg tatccacgtc cttctatcat taaaatatcg
     3481 ttaattttct ccaaaaaggc atcctcccaa gtattgaggt gatagaaaaa gtggggtcac
     3541 agggatttga accctgatcc taggatttct cttgtctcag tactccaatc gatcatcatc
     3601 cgattgtaat acctaacgtc agagcgcgcg tttcttcaaa gacaactgga gtcccagatg
     3661 atggcctggt tacaccatga cccctaaata tatggtttaa agccaaggga gagatttgaa
     3721 ctcccgtgta atggatctgc agtccaccgc ttcgcctcta agctaccttg gctcattagt
     3781 tacgttgcgc actgttgcta tttaaacctt acggaggaaa aataacattt gtttaggaga
     3841 gtctattatt acatatacag attttaagaa tagtaattat gaattaaagc ct
//
LOCUS       DAOQVW010000092         3820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27027_length_3820_cov_2.866932, whole genome shotgun sequence.
ACCESSION   DAOQVW010000092 DAOQVW010000000
VERSION     DAOQVW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27027_length_3820_cov_2.866932"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="PKK85_02905"
     CDS             complement(<1..1481)
                     /locus_tag="PKK85_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNS25000.1"
                     /translation="MTTKIARGSIIMLFGAFIFRMGGAIYRFTMAYLLGPMGYGILSL
                     ATPMQGILIMIASGGMPPAIAKYVAQYSAQDNDEMVKQVIHTATKMVLILGMLFSVAI
                     FVLAEPLAVGLFHKPEAILPFQLVAFITPFSVLVGALRGTFQGKHQMGNIVITKAFEQ
                     VFMIISAIFLVIAGFFVAGAIIGTTIGFIASLIAGYVLYRRGIGLELKNVKLTFNFRQ
                     ELSIAKMLLIFSFPVVITGLAELALFDFVGNFVVGIYLASEYVGYYGIATPIARLPLT
                     ISTAVSTALLPAATEAVSTNDKNLLKTYINQSYRYVTLLVLPLSVGTMFFAAPILNII
                     GPAYVNATSALQILAVGMLFFTLYTVSSSIAQGFGKPYLPMVVLVLGTFLDLGLSLYL
                     VPIYGINGTAIATTISGFFIMAILSWKILKIADVSLSRDNFIRIVAATSILGLFFWIL
                     PKTLPFLMVALILATPVYAVSIIAVGGMKKSDVRAIYTLSNKMG"
     gene            1775..2224
                     /locus_tag="PKK85_02910"
     CDS             1775..2224
                     /locus_tag="PKK85_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HNS25001.1"
                     /translation="MKTVMATGTFDLIHPGHGYYLEESKKLGGKDSRLVVVVARDATV
                     RAKKRVPIVPENQRREVVAMLKPVDQAYLGGETDMFQIVEKLKPDIISIGPDQKFDLE
                     WLKEELKKRNLKAEVIKIDGYLDSDLDSTCKIIKKIKDTGFPAGSFC"
     gene            complement(2320..>3820)
                     /locus_tag="PKK85_02915"
     CDS             complement(2320..>3820)
                     /locus_tag="PKK85_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudomurein-binding repeat-containing protein"
                     /protein_id="HNS25002.1"
                     /translation="TTYNDALVSSIAGMTRDCDIDGIHLDYIRYPGTAYLHPGGTEAI
                     TAFVKRVSETVNSIKPKVALSAALMPEGSVNGYYYGQDYGQLSQYLDFLVPMIYKGNY
                     RQDTSWIGRTTANIVSYSNGKPVISGIQTYKSDDNLVALSASEINQDIQSALSNGASG
                     YALFRYGWLDKTFFQNKITNPETSTTSFTLKQLSEAAGRVKAFVEANKKLPNYVQISS
                     LQVSMPQFLYLLSAATTQVNSGVSSSITPKSVLSPTGPTDDMKNGTIYKSEYVDMASR
                     IKSFIDTNGLAPNYVSTSLGKIRYESMIYLYSRVMDYYGTNARLPNYVSVNTWTSSST
                     ATTTTTDVTDPNAPTNLSAYLQSTKNCQVNDVNIVALSRSITSGATSAYDKGQSIYKW
                     VRNNLEYSWYYNSQKGAVNTLSSLSANCCDHSHLIAALCRAAGVPTRYVHGTCNFSSG
                     TYGHVWAQIYANGKWYDADATSSRNEFGSIYSWDTSSWTFKGIYAELPF"
BASE COUNT         1140 a          756 c          847 g         1077 t
ORIGIN      
        1 cccattttgt tacttagtgt gtagatggct cgaacatcac tctttttcat tccacctact
       61 gctatgatac taacagcata gactggagtc gccagaatca gggccaccat aagaaatgga
      121 agagttttag gtagtatcca aaaaaacagt ccaagtattg atgtagctgc cacgatacgg
      181 atgaagttat ccctggaaag agatacatcg gcgattttca gtatcttcca tgataaaatg
      241 gccatgatga agaatcctga aatggtggtg gcaatggctg taccattaat tccataaata
      301 ggcaccaaat ataagctcag ccccagatcc aggaaggttc ccaagaccag aaccaccatg
      361 ggaagatatg gttttccaaa accctgggct atgctgctgg aaaccgtgta taaggtgaag
      421 aataacatgc ccactgcaag tatctgtagg gcggaagtgg catttacgta agcaggtcca
      481 atgatgttta agataggggc agcgaaaaac atagtcccca ctgatagtgg taggaccaga
      541 agggtcacgt aacggtaaga ctggttaata taggttttta gaagattttt gtcattggta
      601 ctaactgctt cggtagctgc cggtaacaga gcagtggaaa cagccgtaga aatggttaaa
      661 ggaagtcgtg ctatgggagt ggctatacca tagtacccta cgtactcact ggctaagtaa
      721 atacccacca cgaagttacc cacaaaatcg aaaagggcca gttcagccag accagttatg
      781 accaccggaa aagagaatat caataacatt ttggctatgg acagctcctg tctaaaatta
      841 aaagttaact ttacattttt aagttccagg ccaattcctc tcctgtaaag cacgtatcca
      901 gctattaaag atgctatgaa tcctatggtc gtaccaatta ttgctcctgc aacaaaaaag
      961 ccagctatca ctaggaatat agcggatatt atcataaaga cctgttcgaa ggccttggta
     1021 attacgatgt tacccatctg atgttttccc tggaatgtac ccctcagggc acccactaaa
     1081 acactgaaag gggtgataaa tgcaactaac tggaagggta gaatggcttc aggtttgtga
     1141 aataatccca ctgctaatgg ttcggctaat acaaaaatgg ccacactaaa aagcattccc
     1201 agaatgagca ccatctttgt ggcggtgtgt atgacctgct tcaccatttc atcattatct
     1261 tgagcagaat actgggccac atacttggct atagcaggag gcattccccc agaggctatc
     1321 atgatcagta tgccttgcat aggagttgct aaactgagaa taccatagcc cattggccct
     1381 aaaaggtaag ccatggtgaa acgataaata gcgcccccca tccggaatat gaatgcccca
     1441 aagagcatta taatactgcc tctagctatt tttgtggtca tgttacacct tcaaggagta
     1501 gtctatttat gaatcttcac agaatatacc ccaatcttac aaagaaaagg atctttaact
     1561 aaagagagat gaattcacat cttcattatt tataaatgta cttatctatt cattaaatct
     1621 ggtacttgtc tgatataaac tggactaaaa aaccccgatt acaaaaattt atgcaatgag
     1681 atggagtaat taagataggt ttttaaacct ctcattatta atacccataa tatcatgctg
     1741 ttctggttag gaagataata atgaggtgaa taaaattaaa actgtaatgg cgactggaac
     1801 ctttgatcta atccatcctg gtcatggata ttatctggaa gagtcaaaaa agttaggtgg
     1861 aaaagattcc cggttagtgg tagtggtggc ccgtgacgct acagttaggg ccaagaaacg
     1921 agtgccaatt gtccctgaaa atcagcgtcg agaggttgtt gctatgctta aaccagttga
     1981 ccaggcatat ttaggtggtg agactgatat gttccagatc gtggagaaat taaagccaga
     2041 cataatatcc attggaccgg atcagaaatt cgacctagaa tggttaaaag aagaacttaa
     2101 aaagaggaac ctcaaggcag aggtaataaa aattgacggt taccttgatt cagatctgga
     2161 cagcacatgt aagataatca agaagataaa agatacgggt ttcccagcag gaagtttctg
     2221 ttaataattg ttttaaatta gtttttcaaa tatagccgaa gctagtatct tccataattc
     2281 tatttagttt taagtaaaag aaaaaaagaa aaaaaggatt tagaaaggca attctgcata
     2341 gatgccttta aaagtccacg aacttgtgtc ccaactgtag atgcttccaa actcattacg
     2401 ggagctggtt gcatctgcgt cgtaccattt tccattggca tatatttgag cccatacgtg
     2461 tccgtaggtt ccactggaga agttacatgt cccatgtaca taacgagttg gcactcctgc
     2521 tgcccgacaa agggcggcta tcagatgtga atggtcacag cagttggcag aaagactgga
     2581 aagggtgttt accgctcctt tctgcgaatt gtagtaccat gaatattcca agttatttcg
     2641 cacccattta tagatgctct gtcccttgtc ataggctgaa gttgcgccag atgttatgct
     2701 acgtgacagg gctacgatgt taacatcgtt tacttgacag ttctttgtgg attgcagata
     2761 cgcactcagg ttggtcggtg catttggatc tgttacatcc gttgtagtag tagttgctgt
     2821 tgaactggag gtccaagtat ttacactcac gtaattaggt agtctagcgt tggttccata
     2881 ataatccatc acccgggagt aaaggtaaat cattgattcg tatctgattt ttccaagaga
     2941 ggttgatacg tagttaggtg ccaagccgtt ggtatctatg aaggatttta tccgactggc
     3001 catatcaaca tactctgact tgtaaatggt gccatttttc atatcatccg tgggtccagt
     3061 aggacttaac acggattttg gggtgatcga tgaggatact ccgctattga cttgcgtggt
     3121 ggctgcactt agtaggtaca agaactgggg catgctgacc tgtagcgatg aaatttgcac
     3181 atagttaggc agtttcttgt tggcttcgac aaaggcttta acccgaccag cggcttcact
     3241 taattgtttt aaagtgaagc tggtggttga tgtttcaggg ttagttattt tgttctggaa
     3301 gaatgtctta tctaaccagc cgtatctgaa cagtgcatat cctgatgctc catttgaaag
     3361 agcagattga atatcctggt taatttcaga tgcggatagt gcaacgagat tatcatctga
     3421 tttataggtc tgtatcccag atatcacagg ttttccattg gaatagctga ctatgtttgc
     3481 agttgtgcgg cctatccaag aggtatcttg acgatagtta cccttgtaga tcatgggtac
     3541 caggaaatcc aggtactggg aaagttgccc ataatcctgc ccatagtagt acccattcac
     3601 actcccttcg ggcattaatg ccgctgaaag tgctactttt ggttttatgg aattaaccgt
     3661 ttcagacact ctcttcacga aggcagtgat tgcttcagtt ccaccggggt ggagatatgc
     3721 agttcccggg tagcgaatgt aatccaagtg gataccatca atatcacagt cacgcgtcat
     3781 acctgctatt gagcttacca gtgcatcatt gtacgttgta
//
LOCUS       DAOQVW010000093         3816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27064_length_3816_cov_3.056634, whole genome shotgun sequence.
ACCESSION   DAOQVW010000093 DAOQVW010000000
VERSION     DAOQVW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27064_length_3816_cov_3.056634"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKK85_02920"
     CDS             complement(<1..809)
                     /locus_tag="PKK85_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS25003.1"
                     /translation="MSRRTKMINKIKELMYRPEYIRNIGIVAHIDHGKTTLSDNLLAG
                     AGMISAELAGDQLYLDFDEQEQARGITIDAANVSMVHKYHDNEYLINLIDTPGHVDFG
                     GDVTRAMRAVDGAVVVVCAVEGIMPQTETVLRQALKENVRPVLFINKVDRLINELKLD
                     PEELQQRFIKVIANANKLIKNMAPEELKKKWQVRVEDGSVAFGSAYHNWAINIKLMQE
                     TGINFKDILEYCNNDNQKELAQQAPLSDVLLGMVVEHLPSPDIAQKYRVPS"
     gene            complement(833..1396)
                     /locus_tag="PKK85_02925"
     CDS             complement(833..1396)
                     /locus_tag="PKK85_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNS25004.1"
                     /translation="MSFKVFDKWDLMEVKVEDMGLVNYVCIDEIMVPHTMGRHVRRQF
                     AKSRVSIVERLMNKIMRTERNSGKKNKSYNIVKDSFDIINKRSKQNPVQVLVKAVENT
                     APREETTRIKYGGIGYQVAVDIAPQRRVDLALAFLTRGALQSAFKRKKSAQECLAEEL
                     LLAAEYDTRSFAIQKKEEKERVARSAH"
     gene            complement(1407..1832)
                     /locus_tag="PKK85_02930"
     CDS             complement(1407..1832)
                     /locus_tag="PKK85_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019267857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNS25005.1"
                     /translation="MPGLFAAKKLKKNRQHFRWKDMYYKRKQLRLDVKADPLEGSSQA
                     RGIVIEKVGIEAKQPNSAIRKCVRVQLIKNGKQITAFAPGDGAIGFIDEHDEVLIEGI
                     GGGSGRSRGDIPGVRWKVTKVNNVSLEEMVRGKIEKPVR"
     gene            complement(1884..2315)
                     /locus_tag="PKK85_02935"
     CDS             complement(1884..2315)
                     /locus_tag="PKK85_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HNS25006.1"
                     /translation="MTIKFTTNEIRYIALFESMTGATVKDCLVDEENGKLTFLVKNGD
                     MGLAIGKRGSTVSKVQKTVDKGVEVIEHSDDQVEFLSNLMSPAKLRSIRILQRENGEK
                     IATIEADSRNKRTAIGKGGQNIERARILAKRQHNISNIVIK"
     gene            complement(2321..2617)
                     /locus_tag="PKK85_02940"
     CDS             complement(2321..2617)
                     /locus_tag="PKK85_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HNS25007.1"
                     /translation="MDVDRGIRVAVDTGSVTLGSEKTVQALKLGKGKLVIIAENCPRE
                     IREDVEHYSQLSKIPVYTYEGTSVDLGSVCGKPFTVAAMIISEPGDSTILELMG"
     gene            complement(2629..3783)
                     /gene="rpoA2"
                     /locus_tag="PKK85_02945"
     CDS             complement(2629..3783)
                     /gene="rpoA2"
                     /locus_tag="PKK85_02945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HNS25008.1"
                     /translation="MDIKKVEKAVKKKKADFPEQLIQEITEAAKRHELKAGELDELVD
                     TLKKAYERAEVESGEAVGTVAAQSVGEPGTQMTMRTFHYAGVAELNVTLGLPRLIEIV
                     DARKKISTPTMAIYFEEDYAEDEEYVRTLANRIGKITLNDILMDFNVNYASMTLSIEI
                     DEEQIEEKRLDYDEIIKKIEKAFKKVEINKNLLGFEPTISEAKHAIRELRLLADKVRD
                     LQISGIKNIGKVVIRKEGQEWVIHTEGSNLGAILKMEGVDKTRTTTNDIHEVEKILGI
                     EAARNAIIREAQATMEEQGLTVDVRHIMLVADMMTADGTVKSIGRHGISGEKASVLAR
                     ASFEETGKHLLRASIRGEVDHLTGIIENIIIGQPIPLGTGSVGVIMKQRE"
BASE COUNT          828 a          997 c          752 g         1239 t
ORIGIN      
        1 atgctgggca cccggtattt ttgggctata tcagggctgg gtaagtgttc taccaccatt
       61 ccaaggagca cgtcagataa aggagcttgt tgggctaatt ctttttggtt gtcgttgtta
      121 cagtactcta agatgtcctt gaaatttata ccggtttcct gcattaattt gatattaatg
      181 gcccagttgt ggtaggctga tccaaaggct acactgccat cctccacccg gacttgccac
      241 ttcttcttga gttcctctgg ggccatgttc ttaatgagct tgttggcatt ggcgatgacc
      301 ttaataaacc gttgctgcag ttcttcaggg tccagtttaa gctcgtttat gagccggtcc
      361 accttgttga tgaacagtac tggccgtaca ttttccttaa gggcttgtct cagcacagtt
      421 tcagtctggg gcatgatgcc ttctacggca cacaccacta ccaccgcacc atccactgct
      481 ctcatggcac gggtcacatc tcctccgaag tccacgtggc caggagtgtc tatgaggttg
      541 atgaggtact cgttgtcgtg gtacttgtgc accatggaca cgttggcagc gtcgatggtt
      601 ataccccggg cctgttcttg ttcatcgaag tccaagtata actggtctcc agccaactca
      661 gcagatatca tacccgcacc tgccaaaagg ttgtccgata gggtggtttt tccgtggtca
      721 atgtgggcca cgataccgat gttccggatg tattcaggcc ggtacatcag ttccttgatc
      781 ttgttgatca ttttagtgcg tctgctcaca cgtatcacct aataaatctt ggttaatggg
      841 cagatctagc caccctttct ttttcttcct ttttctgtat ggcgaagctt ctggtatcat
      901 actcagcagc cagcagcaac tcctcagcca gacattcctg ggcagatttt tttcgtttga
      961 aggcagattg taatgctcct ctggttaaaa aggccagagc taagtccact ctcctctggg
     1021 gggctatgtc cactgctact tggtatccaa ttccaccgta cttgatacgg gtggtttctt
     1081 ctcgaggtgc tgtgttttcc accgctttga ccaggacttg aaccgggttc tgtttggaac
     1141 gcttgtttat aatgtcaaat gaatctttaa caatattata ggacttgttc tttttaccag
     1201 aatttcgttc tgtcctcata atcttgttca tcaatctttc cacaatggaa actctggatt
     1261 tggcgaattg tctcctgacg tgacgcccca tagtatgagg aaccattatt tcgtctatac
     1321 agacgtagtt aaccaaaccc atgtcttcca ccttcacctc catcaggtcc cacttatcaa
     1381 aaactttaaa actcataaaa ttacctctat ctaactggtt tttctatttt accccggacc
     1441 atctcctcca aggatacgtt gttaacttta gtgactttcc agcgtactcc agggatatct
     1501 ccccgggatc ttcctgatcc tccacctatt ccttcgatta acacttcatc gtgttcatct
     1561 atgaatccga tagccccatc tcccggggcg aaggcggtga tctgtttacc gttttttata
     1621 agttgtaccc tcacacattt ccggatggcg gagttaggct gtttggcctc gatacccacc
     1681 ttttcaatca ctatacccct ggcttgggat gaaccctcta atggatctgc tttaacgtca
     1741 agacgtagct gtttcctctt gtagtacatg tccttccacc tgaagtgttg tcgattcttt
     1801 ttaagctttt ttgctgcaaa cagtcctggc aaatatgatt cctccttaaa taaaaactta
     1861 tacagtttta gcttgattat ctcctacttt attactatgt tactaatgtt gtgctgcctt
     1921 ttggccagga tccgggctcg ctctatattt tgacccccct ttccaatagc agttctttta
     1981 ttgcgagaat cagcctcaat agtggctatt ttctcgccat tctccctttg aagtatcctt
     2041 atgcttcgaa gtttggcggg agacattagg ttgctgagaa actccacttg atcatcggag
     2101 tgctcgataa cttccacacc tttatccacg gttttctgga ctttggatac agtacttccc
     2161 ctcttaccaa tggccaagcc catatcacca tttttgacca gaaaggtgag tttaccgttt
     2221 tcttcatcca ccagacaatc cttgactgtg gccccagtca tactctcgaa cagggcgatg
     2281 tatcttatct catttgtagt gaatttgatg gtcacaaaaa ctaccccatt agttctagta
     2341 tggttgaatc tccgggttcg gagatgatca tcgcagccac ggtgaatggt ttaccgcaca
     2401 cggaacccag atccacactg gtgccctcgt aagtgtaaac cggaattttt gataactgtg
     2461 agtagtgttc cacgtcttct ctgatttccc gggggcaatt ttcagctatg atgactagtt
     2521 ttccttttcc aagtttcagg gcctgtactg tcttttcgga ccccagtgtg acacttccag
     2581 tgtctactgc tactcgaatt cctctatcta cgtccatgga atgcctcctt attctctctg
     2641 tttcattatt acgccaactg accctgttcc cagtggtatt ggctgaccaa ttatgatatt
     2701 ttcaattatt ccggtgagat gatccacctc gccccggata ctggccctta acaggtgttt
     2761 tccagtttct tcaaatgatg cccgggcaag tacactggcc ttttcaccgc tgataccatg
     2821 acgtccaatg gatttgacag ttccatcggc ggtcatcata tccgccacca gcatgatgtg
     2881 ccggacgtcc accgtaagcc cttgctcctc catggtagcc tgagcttctc ggattatagc
     2941 attacgggcg gcttcaattc ccagaatctt ctccacctca tgtatatcgt tggtggtagt
     3001 ccgggtctta tccaccccct ccatcttaag gatggcccct aaatttgaac cttcagtatg
     3061 aataacccat tcctggcctt ccttcctgat tacaactttt cctatgttct tgataccact
     3121 tatctgcaaa tcacggactt tatccgctaa aagtcgtaac tctctgatgg catgtttagc
     3181 ttcagaaatc gtaggttcaa aacctaacag gttcttattt atctccacct ttttaaaagc
     3241 tttttctatc ttctttatga tctcatcata gtccagtctt ttctcttcga tttgctcttc
     3301 atcgatctca atggaaagtg tcatactagc gtagttgacg ttgaagtcca tcagaatgtc
     3361 gttcagggtt atctttccta tgcgattagc caaggttctt acatactctt catcctcagc
     3421 ataatcttct tcgaagtata tggccatggt tggtgtggat atcttcttcc gggcatctac
     3481 aatttcgata aggcgaggca accccaaggt aacgttcagt tctgccaccc ccgcgtagtg
     3541 gaatgtacgc atggtcatct gggtaccggg ttctcccacg gactgggcag ccaccgtacc
     3601 cactgcttca ccggactcca cctccgcccg ttcataggct tttttaaggg tatccaccag
     3661 ttcatccagt tcaccagcct ttaattcatg tctcttcgct gcctcagtga tttcctggat
     3721 tagttgctct ggaaaatcag cttttttctt cttaaccgcc ttttctacct ttttaatatc
     3781 cacataacca ccttagttgc ctatttagta cccaat
//
LOCUS       DAOQVW010000094         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27228_length_3798_cov_2.847716, whole genome shotgun sequence.
ACCESSION   DAOQVW010000094 DAOQVW010000000
VERSION     DAOQVW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27228_length_3798_cov_2.847716"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PKK85_02950"
     CDS             complement(<1..179)
                     /locus_tag="PKK85_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatinine amidohydrolase"
                     /protein_id="HNS25009.1"
                     /translation="MIKLRYSSGNIISPDVHKIGVLALGSHLENHGAALPIDTDSKIA
                     AHIALQASLRTGAAF"
     gene            complement(231..707)
                     /locus_tag="PKK85_02955"
     CDS             complement(231..707)
                     /locus_tag="PKK85_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNS25010.1"
                     /translation="MRIKKRYHLKKKYLKEVKEKLGIYSDLLPDNVQIEMLETDVEDL
                     ILVDGEPLIMVLDGIPYPALKGVLQRFSESHYVVVDRGAVKFVANGADVMSPGIVEAD
                     PQIKEGDVVIVIEESHRKPLAIGRALISGKAMVESQQGKAVKTLHYVGDKIWSLEV"
     assembly_gap    759..768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            808..1050
                     /locus_tag="PKK85_02960"
     CDS             808..1050
                     /locus_tag="PKK85_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LSm family protein"
                     /protein_id="HNS25011.1"
                     /translation="MSGQKNVNTSRPLDVLGRSLNSQVLIKLKGGREFRGVLESFDMH
                     MNLVLNDAEKLEDGESTTRLGVVLIRGDNIVYISPG"
     gene            1105..1287
                     /locus_tag="PKK85_02965"
     CDS             1105..1287
                     /locus_tag="PKK85_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HNS25012.1"
                     /translation="MKGTPSFGKRNKKTHIRCRRCGKNSYNARKRYCAACGFGRSSRI
                     RTYNWQNKKINGYRLK"
     gene            1288..2178
                     /locus_tag="PKK85_02970"
     CDS             1288..2178
                     /locus_tag="PKK85_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNS25013.1"
                     /translation="MDTRNPIDVRIIVEGASDVENISRALQDIALGAEYHITISSIVP
                     TTSPKIAKKAVAGADIVLIATDVDAPGRELAEKFHKILKDKVGHVERMKLPFGHDVEY
                     IDPALIRREIKNAIIRSGLIAIANINHFQEVMDQLHQSEETIDEIQKENEELLLLNEK
                     LVSQSQELSSSQELLESKLKSLKEEFKVLKQKYADIKNKQSMLLNKNLFETFSIQKLW
                     NEEFNEDLTVEEQVIFITNEFKPDNILIGQGHIAAPSRKEATEWLKIIRTVLIFYDSK
                     LEDLKEEMEEDKFGLQLLKE"
     gene            2244..3641
                     /gene="purF"
                     /locus_tag="PKK85_02975"
     CDS             2244..3641
                     /gene="purF"
                     /locus_tag="PKK85_02975"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNS25014.1"
                     /translation="MQDKCGIVGAYSLNKSKNVSRDIYYGLYALQHRGQESAGISSHN
                     SGQMCTYRGMGLVCDVFNNGNVEELKGHVGIGHVRYSTTGKSFLENSQPFMTEFESGE
                     ISVAHNGDIINSSELRQELEKKGIQFCSTTYSEVLCHLLTEEYQNTENMVESIKKVSK
                     QIIGSYSLVILLNDDLIVVRDPIGIKPLAMGKINQTTMVASETVAFDAIGANYVRDVE
                     PGEILLINDEIKSYQIPRNKNTPRAHCMFEYVYFARPDSILDGRSVYNVRLNIGKSLA
                     QQYPVEADVVMPIPDSAITAAIGYSRESGLPYGEGLIKNRYVGRTFIMPTQEERENSV
                     RLKMNPIRSELQGKRIVIVDDSIVRGTTSKALVEVIRDAGVEEIHLRIGCPPIISPCY
                     YGIAMATKKELIASDKKAEEIRKILGVDSLGYLSAEALVECIGIKRDELCMGCLTGDY
                     PTDLPDDLEDYEACR"
BASE COUNT         1171 a          725 c          816 g         1076 t
ORIGIN      
        1 aaaaatgcgg ccccggtccg taaagatgct tgcagtgcaa tatgggctgc tattttagag
       61 tctgtatcta ttggtaatgc ggctccatgg ttttccagat gggaaccgag agctagtacg
      121 cctattttat gcacatctgg tgatataata tttcctgaag aatatctaag ttttatcatg
      181 cttcttcctg taaaatgcgt ttttttattc taaaaatttt aaatccactt ctaaacttct
      241 aaactccaga tcttatcccc cacataatga agcgtcttaa cggccttacc ttgctgactt
      301 tccaccattg cttttccaga aatcagggca cgaccaatgg ctaaaggttt tctgtgggat
      361 tcttcaatga cgatcacaac gtcaccttct ttgatctggg gatcggcttc cacaataccc
      421 ggggacatga catctgcacc gttggccaca aatttcacag caccccgatc caccaccaca
      481 taatgtgatt ctgaaaaacg ttgtaaaact cctttcaggg caggataggg tattccatct
      541 aggaccatta ttaaaggctc accatctacc agtataaggt cttccacatc tgtttctagc
      601 atttctatct gtacattatc aggtaagagg tcagagtaaa tgccaagctt ttcctttacc
      661 tctttgaggt atttcttttt aaggtggtag cgttttttta ttctcaaata acaacaccgc
      721 attagctttt cttccctatc atcttaatat ttttaagtnn nnnnnnnnaa atctctacta
      781 catgatggta aaggaaaggt gataatagtg agtggacaga agaatgttaa cacttcccga
      841 ccactggatg ttttaggtag atcactaaac tcccaggtcc tcataaaatt gaagggtgga
      901 cgtgaattca ggggagttct agaaagcttt gacatgcata tgaatttggt cttaaatgat
      961 gctgaaaaat tagaagatgg agaatcaacc actcgtctag gggtggtact catacgtggc
     1021 gataacatcg tctatatatc acctggatag tttggatcta ccgggataag aaagaatagt
     1081 attaattttt gttggaggta aataatgaaa ggtacaccct catttggtaa gcgtaacaaa
     1141 aaaacacaca taagatgccg taggtgtggt aaaaactctt acaatgcccg gaagaggtac
     1201 tgcgccgcct gcggatttgg aagatccagc aggatacgca cttacaactg gcagaacaag
     1261 aagattaatg gatataggtt gaaatagatg gacacgagaa accccatcga tgtgcgtatc
     1321 atcgtggaag gagcttccga tgtggaaaat atctcccggg cccttcaaga cattgccctg
     1381 ggggctgagt accacatcac catctcctcc atcgtaccca ccaccagccc taagatcgct
     1441 aaaaaggcag ttgctggtgc tgacatagta ctgattgcaa cagatgtaga cgcacctgga
     1501 cgggaacttg ctgaaaagtt tcataaaatt ctaaaggata aggtggggca tgtagagcgc
     1561 atgaaactac cctttggaca cgatgtggaa tacatagatc ctgcccttat ccgtcgggag
     1621 ataaaaaacg ccattattcg ttccgggctc attgccattg ccaacattaa ccatttccag
     1681 gaggttatgg atcaactcca tcagtctgaa gagactatcg atgaaattca gaaagagaat
     1741 gaagaactct tacttttaaa tgaaaaactg gtttcacaga gtcaggaatt atcatcctcc
     1801 caggaactcc tggaatccaa attaaaaagt ttgaaagagg aatttaaggt tttaaagcaa
     1861 aagtatgctg atataaaaaa taaacagagc atgttgctta ataaaaacct tttcgaaact
     1921 ttttctatac aaaaactctg gaatgaagaa tttaatgaag atttaactgt ggaagaacaa
     1981 gttatattta ttaccaacga atttaagccg gataatatcc tgataggtca ggggcacatt
     2041 gctgctccct ccagaaaaga agcaaccgag tggttgaaga ttattcgcac tgtactgatt
     2101 ttttacgatt ctaaacttga ggatcttaag gaagaaatgg aggaagacaa attcggcctt
     2161 caacttctta aggaatgaaa ttttcgaaga agatttcata ttgattctaa attagtaata
     2221 tcatagtacc gggagaactt gaagtgcaag ataaatgtgg tattgtaggt gcttattctc
     2281 ttaataagtc caaaaatgta tctagagata tctattacgg cctttacgcc ttgcagcatc
     2341 gtggacagga atctgctggc atatcttccc ataacagtgg acaaatgtgc acttaccggg
     2401 gaatggggtt ggtctgtgat gtttttaaca atggaaatgt ggaagaactg aaaggtcatg
     2461 tgggaatagg acatgttcgt tattctacca ctggtaaatc ttttttagag aattcccagc
     2521 catttatgac tgaatttgag tcgggagaaa tttcggtggc tcataatggg gatattataa
     2581 actcttcaga gcttcgccag gagttggaaa agaaggggat ccaattttgt tccaccacct
     2641 attcggaggt gctctgtcat ctgttaactg aggagtacca gaatactgag aacatggtgg
     2701 agtcaattaa aaaagtatca aaacagatta taggttccta ctctctggtt atcctattaa
     2761 atgatgatct gattgtggtt cgagatccta taggcataaa acccctggcc atggggaaaa
     2821 taaaccagac cactatggtg gcatcagaaa cagttgcttt tgatgccata ggtgccaatt
     2881 atgttcggga tgttgaaccc ggtgaaatac tacttataaa tgatgaaatt aagagttatc
     2941 agatacctcg gaataagaat actccacgag cacactgtat gtttgaatat gtttattttg
     3001 cccgaccaga cagcatcttg gatgggcgtt cggtctacaa cgtgagatta aacattggaa
     3061 aatccctggc tcaacaatac ccagtggagg cagatgtggt tatgcccatc ccggactcgg
     3121 ccatcactgc agctatagga tattcacggg aatctggatt accatacgga gaagggctta
     3181 tcaaaaatcg ctacgttggt cgaaccttta ttatgcctac ccaagaagaa cgagaaaact
     3241 cagttcgtct taaaatgaat cccatacgtt cagaactgca gggtaaacgt atcgtaatag
     3301 tggatgacag catagtcagg ggaaccactt ccaaagcatt agtggaagta atacgtgatg
     3361 ccggagtgga agaaattcat cttagaatag gttgtccacc tatcatttcc ccctgttact
     3421 acggcattgc catggccacc aaaaaggagc taattgcctc ggataagaag gcggaagaaa
     3481 tccgaaaaat attgggggtt gattccctgg gatatctgag tgcagaggcc ctggtggagt
     3541 gtattggtat aaagagagat gaattatgta tgggttgttt gactggggac tatcccaccg
     3601 atttaccgga tgacttagag gattacgagg cttgccggta atagataggt tttttttaca
     3661 gttgagtttt aactttttgg ttttatctct tgaaaacaaa ttattaggta ctgtaatgta
     3721 tatttttgat caataaagta tatatcattc caataacaat ctgttagtta aggaaacaga
     3781 tcacaaacta ccaatcct
//
LOCUS       DAOQVW010000095         3786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27374_length_3786_cov_2.311713, whole genome shotgun sequence.
ACCESSION   DAOQVW010000095 DAOQVW010000000
VERSION     DAOQVW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27374_length_3786_cov_2.311713"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKK85_02980"
     CDS             complement(<1..383)
                     /locus_tag="PKK85_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25015.1"
                     /translation="MKFKRNIHQKRTISMITLVVLLPVIGIIYSPILMGMGLKNGFIT
                     SSAIIFSIWLVLSLFIGRAASCGYTCPYGAIQEITGGWILNRRVRNPRLAQLKYLVFI
                     IFMTSVAFSIFKLGGLKGVNFFALN"
     gene            complement(887..2050)
                     /locus_tag="PKK85_02985"
     CDS             complement(887..2050)
                     /locus_tag="PKK85_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25016.1"
                     /translation="MNEITRLKTDLLCKGININPKELYYREIVPRAYAGAGKSRAGVA
                     GSGKSFILEHQEPCNIGVLQPLLEESPYQFKIIDNQGWILKNGEKVIKATLREPFWHK
                     TPHSDLIQLHGKDSLATALSNFCTYKKHGDGCKFCIIDIGNQVVFHHPQQVARSIQYI
                     EENPELRACFNLEDKYNPDLTDKEILKRKIYIKPMDININAGALKNVARIYGNTVSEI
                     GKVSQLPIFMEVGPLEREEIEDLHSKGIDAASFNLELYDPKRRKEVIPGKARRNSLDA
                     YLSSMRNAVDILGENQVSSWILIGLESPDKTIEGIRRIAETGAIPLPKPFRPLYSADY
                     EKKRPPNLKDAVRIYQEWLDTVRECGLNPLKAKAGCARCNACFPLKELLKYGV"
     gene            complement(2418..2780)
                     /locus_tag="PKK85_02990"
     CDS             complement(2418..2780)
                     /locus_tag="PKK85_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HNS25017.1"
                     /translation="MVKWGPVIVGFILAVILGNLFGVVSNAYWGVNLGLFIAGFIVGY
                     WVHEGIWGGLWNATVAGAFGSIVIAIILIIGGTIFGGLAGLAAGILTGLTMIIVSLIA
                     NIVAMGVGGAIGGLLSGD"
     gene            complement(3184..>3786)
                     /locus_tag="PKK85_02995"
     CDS             complement(3184..>3786)
                     /locus_tag="PKK85_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNS25018.1"
                     /translation="NILTPFSQILRNKLDEVLSLNLQIDLIATSHGVVWRDNPMQIVE
                     KYSKWADDYQEDQITIIYDTMWNGTKILAEKIAEGIALADPEVVVKVLNLAKSDENDL
                     ITEVFKSKTVLMGSPTISNTILHSVAGFIHLMKQLKFKNKKAAAFGCYGWSGESVKVL
                     NDSMENAGFEVIEEGFKNHWNPDVDAQEAALEFGKKIALS"
BASE COUNT         1032 a          858 c          718 g         1178 t
ORIGIN      
        1 ctatttaatg caaaaaagtt tactccttta agtcccccca gcttgaatat ggaaaaggcc
       61 acggaagtca tgaagattat gaataccaaa tattttaatt gggccagcct tggatttcgg
      121 accctcctat ttaaaatcca gccacctgtt atttcctgaa ttgcaccata ggggcaggtg
      181 taaccacagg aagctgctct tcctataaaa agtgacaaga caagccatat actaaaaatt
      241 atggcactgc tggttatgaa cccgtttttt agtcccatgc ccattaaaat tggtgagtag
      301 ataattccaa ttaccggtaa cagcaccacc agagtgatca tggaaatggt tctcttctgg
      361 tgaatattac gtttaaactt cattattctc atatttaact ccagaaacat cattaaatct
      421 attttaatag tattttactg attattatga tttaaaacca aattttagta tttatccttc
      481 caccatctca tttttcagcc taatattatt tatacccttt gtaataagac aatataaacc
      541 aaaaaaatag ggttaaagag tataccatac aagcatcact tagaggatct caggataatg
      601 ggccttgcgg agtacgcatc ccaagcatta ccatggcact gcttccatta tatattatgg
      661 ctggtgcatc tggaataagg gaaacaccta ccatcctaag atccaggact ttaagtctat
      721 tataactaaa ttagaatcat tttctcatta ttaattaatt ataagcgttg atgttgttcc
      781 catagttttt tttaggatgc agttatcaga tcttttaatc tgggcagtca ctcttttgag
      841 ttccccaaat atactgcacc acctgtttta gacatcaagc tccgggttac accccatact
      901 tcaacagttc ctttaagggg aaacaggcgt tacaccgagc acacccggct ttagctttta
      961 aaggatttaa tccacattct ctaacagtgt ccaaccattc ctggtagatc ctgactgcgt
     1021 ctttcaaatt tggtgggcgt tttttttcat aatccgcact gtacagtgga cggaaaggtt
     1081 tagggagtgg tatggccccg gtttctgcta tcctccttat tccctctatg gtcttatctg
     1141 gggattccag accaatcaga atccaggaac tgacctgatt ttcgccaaga atatccaccg
     1201 cgtttctcat ggaactcagg taggcatcca gtgagtttct tcgggccttg ccagggatga
     1261 cctcttttct tctcttcggg tcataaagct ccaggttgaa actggctgca tcaatgcctt
     1321 tagaatgtaa atcttcgatt tcttcccgtt caagtggccc gacctccatg aatatgggta
     1381 gctggctgac ctttccaatc tcagatacgg tgttcccata gatcctggca acgttcttaa
     1441 gggccccagc gtttatgtta atatccatgg gcttgatgta aatttttctt tttaagatct
     1501 ctttatcagt taaatctgga ttgtacttat cttccaggtt gaaacaggcc cgtagttccg
     1561 ggttttcttc gatgtattgt atggaccgag ccacctgttg ggggtggtga aaaaccacct
     1621 ggttccctat atcaattatg cagaacttac aaccatctcc atgttttttg taggtgcaga
     1681 agttggacag ggctgtggcc agggaatcct tgccatgcaa ctgtatcaag tcagaatgtg
     1741 gtgttttatg ccagaatggt tctcttaagg tggctttgat gactttttca ccatttttaa
     1801 gtatccagcc ctgattgtcg atgatcttga attggtaggg cgattcttcc aggaggggtt
     1861 ggaggactcc aatgttacag ggctcctggt gttctaagat gaaggatttc ccactgccag
     1921 ccacacctgc ccttgatttg ccagctccgg cataggctct ggggactatt tcccgataat
     1981 agagttcctt tgggttaatg tttattccct tacagagcag gtcagtctta agtcgggtta
     2041 tttcattcat gatccaggta caatcatttt cgcagaaatt tccttaaaaa ccatccattt
     2101 tcttgggaga aataccttgg cctggaagat ctgcaccatt tctttctcta aacatggggt
     2161 gtgtagattg gattcctgtg aattcctgtg gatacttcaa catcatcctt tggtgtaatg
     2221 cagaagcaca taacggctaa aagattcatt ccatgtttta aatgaaaata ttatctaatt
     2281 ggatcttggg tcatccttca ggttcgttga aatttatcat gcagtggcca cttaggtact
     2341 ggtcctgccc ttctcttaaa aggaccgttc tctccacatt gtggaccttt aagataaatt
     2401 gtggattcag ccatcggcta atctccactc aaaagaccac ctattgctcc cccaacaccc
     2461 atggctacga tgttcgcaat taaggaaact atgatcatgg ttaggccggt tagaattcct
     2521 gctgctaatc cagccagacc accaaagatg gttcctccaa tgattaagat aatggctatc
     2581 actatggaac cgaaagcccc agccacggta gcattccaca aacctcccca gataccttca
     2641 tgaacccaat agcccacaat aaatcctgca ataaacagcc ccagatttac tccccagtag
     2701 gcattgctga caactccaaa caagttaccc agtatcactg ccaagataaa cccgacaatg
     2761 acaggacccc attttaccat ataattcccc cttatctttg atatttagta attgtattat
     2821 ggaattaaaa ctatctaaac ctttggatta atcttcgaag tcaatttcct gattcccggc
     2881 aagcgattct ttctcccagt cttttttggc caaccaagaa acactattgc cactccaatg
     2941 tgtgacaatg atccaggccc atatacacaa attgatactt ggttgaaggg attatcatag
     3001 cacaataata ttgatggatg ttattgtgga ttctcctctg caaagggccg cagcgatcta
     3061 aatggtctgc tgattaaaag gacaccgcta aatcaaaaaa aggaccaccc ctaccctgta
     3121 gagtaagtag cctaataaaa aaaagtgggg atggtgttta atgttaccct ggtatctgaa
     3181 attttatgac aaggcaattt ttttaccaaa ctccaaagca gcctcttgtg catcaacatc
     3241 cgggttccaa tgatttttaa agccctcttc aatgacttca aaacctgcat tttccattga
     3301 atcgtttaaa accttcactg attccccact ccaaccgtag catccgaatg ctgctgcttt
     3361 tttattttta aactttaact gtttcatgag gtggatgaag ccagccacgc tatgcagaat
     3421 agtgttggaa atggttggtg aacccatcag cacggttttt gatttaaaaa cttcggttat
     3481 gaggtcgttt tcatcacttt tggccaggtt gaggactttc actaccacct caggatctgc
     3541 cagggctatt ccctccgcta ttttctcagc cagtatcttt gtaccgttcc acatggtgtc
     3601 gtagataatt gtgatttggt cctcctggta gtcatcagcc cacttggagt acttttcaac
     3661 tatctgcatg ggattgtctc tccagacaac gccatggctg gtggcaataa gatcaatttg
     3721 aagattcaaa gaaaggactt catccaactt atttcttaat atttggctga aaggcgtcag
     3781 aatgtt
//
LOCUS       DAOQVW010000096         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27406_length_3783_cov_3.424624, whole genome shotgun sequence.
ACCESSION   DAOQVW010000096 DAOQVW010000000
VERSION     DAOQVW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27406_length_3783_cov_3.424624"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1062)
                     /locus_tag="PKK85_03000"
     CDS             complement(91..1062)
                     /locus_tag="PKK85_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016358218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HNS25019.1"
                     /translation="MNVNIVTPYLGLLISSGICGIVAFLITFISMPRLIRKLKSAEIV
                     GKDIHKPSKPLVAEMGGIGILFGFAMGMFLGIYLHPLLQGQLIISLLVILLVGVVGMV
                     DDLVQLSSREKLILLWLAGLPLIWIAPPNVGFFYMLSMPFAVTIASNLTNMLAGLNGI
                     ESGLGAIAMTSLSVSCIIMGKIDVALISMAMLGALLAFLYYNRHPSRVFPGDVGTLII
                     GATIVVVAFIGRVKIIALIVLLPNIIDSLLKFFSAGVMERQYQEPTQVGEDGVLLVPE
                     GGFNSLIRWILKRPMKEKNVVILVWSIGMFFGLLGIFLAYFLKTSLI"
     gene            complement(1127..1585)
                     /locus_tag="PKK85_03005"
     CDS             complement(1127..1585)
                     /locus_tag="PKK85_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HNS25020.1"
                     /translation="MVEEIYVSQPLTIQRIMELLEQHPDLVKIKCPPSLYARTSSRYL
                     GALENLGVVVEPEKRRGRPAKYGDEEVKKIDELLSQGKTPREIARLTGIPLKSVYPLI
                     QKNLSPGRPRKYDAETVDRVKKLRKSGKSAREISEQLLIPLRSVYFLLNR"
     gene            complement(1638..3191)
                     /locus_tag="PKK85_03010"
     CDS             complement(1638..3191)
                     /locus_tag="PKK85_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF530 domain-containing protein"
                     /protein_id="HNS25021.1"
                     /translation="MNESLLIGRAEKFLSQIHGFDGKVGDVHSFLGTYSQFKDNLDRL
                     YELRDYMELKGYKAPFRSLKYGRVQSSEMKMDDLHDVSRHSQFFRMRAAAKKNILDRV
                     KSAIASHRIILGHLEEYATVTCTSCQSRYRGHELGKLYPAKCGCGSSQFNLDINKDGF
                     YRLGILKYLPISGDYMVKMSELSSLGREAFRGLVRILKQEKRGIVKTISMVVKVMEDG
                     RWIRKRVKIDAQDQLNYEKRIRESYGNNARIEFIQFHRKRPAIINDKHVQTALALGYV
                     GYSEDLGESLLPPLLQQKLKNESNLLIYDASLKKAEERAKIQKEWDDNLNLKEDLLRE
                     ILQEEGLVDGEGQMNPGLKEDLQLRDKLQKDVFQENPRALILWDLMRYYLSTSYDRRS
                     KYSGPFPYLRPSLDINQLKAFQEFPSQTVTMLKDFQDEDIESLLEMDKIISFKFSLEK
                     KTQGLHIKMDPAMGAAILNIKGQVPISRAAQIFKVTPAQVKKQKVRLETLQKPGSPKA
                     QKFLKLVQG"
     gene            complement(3188..>3783)
                     /gene="metG"
                     /locus_tag="PKK85_03015"
     CDS             complement(3188..>3783)
                     /gene="metG"
                     /locus_tag="PKK85_03015"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNS25022.1"
                     /translation="SNPQEAATCLYLCNQIAKAIGILLLPYLPGKAGDILSILNLDDP
                     EYSWDDAAQFLPAGHVLAPARPLFPKIDDEVIEREKQILYENLEEKEPMDLVSIEDFA
                     CMDLRVGLITGAEAVKGSENLLKLLVDLKDRHIQVVAGLAKSYQPAELINQKVTVLVN
                     LKPAKLFGIKSEGMVLATQDSLNLLDPGSAEVGEKIK"
BASE COUNT          957 a          945 c          821 g         1060 t
ORIGIN      
        1 aacagcgtat ttaatccagc tttctcccac cgctactatt acaccaccgc cgacgtaaaa
       61 tggtgttaac ccccctttga ggaaaaccat ctatataagg ctggttttta aaaaataagc
      121 caaaaagatt cctagcaacc caaagaacat accaatactc caaaccagta tcaccacgtt
      181 cttctctttc atgggacgct ttaaaatcca gcgtatcagg gagttaaaac caccctccgg
      241 caccagcaat acaccatcct cccctacctg ggtgggctcc tggtattggc gttccatgac
      301 cccagcactg aaaaatttga gtaaactgtc gatgatgttg ggtaaaagta cgataagggc
      361 aatgatctta actctaccaa taaaggccac tacaacgatg gtggcaccta tgatgagggt
      421 gcccacatcc ccggggaaaa cccttgaggg gtggcggttg tagtacagga aggccaggag
      481 cgcccccagc atggccatgc tgattagggc cacatctatt ttacccatga tgatgcacga
      541 aactgataga gaagtcatgg caatggcccc taatcctgat tcaataccat tcaaaccggc
      601 cagcatgttg gtaaggttgg atgcaatggt aacggcgaat ggcatggaca gcatgtagaa
      661 gaaccccacg ttcggtggcg cgatccatat gaggggcaat cctgccagcc atagcaggat
      721 tagcttctcc cgggaggaaa gctgcactaa atcatcaacc atccccacaa caccaaccag
      781 tagaattacc agtaggctaa tgattagctg tccctggagt aggggatgta aataaattcc
      841 cagaaacatc cccattgcaa aaccgaataa gatacctata ccacccatct ctgccaccaa
      901 tggcttgctg ggtttatgga tatccttacc aacgatttct gcactcttaa gcttccttat
      961 gagccggggc atgcttatga aggttattag aaaggccaca attccacaga taccagagga
     1021 aattaggagg cccagatagg gggttacgat gttaacattc attttccatt ctcctacagg
     1081 gaagataact cccctgccta ggcaaagata cttactgtaa tggctgtcac cggtttaaaa
     1141 gaaagtacac actccgcagg gggatgagta gttgttctga tatttccctg gcacttttcc
     1201 cagatttacg gagtttttta accctatcca cagtttcagc gtcgtatttc cgtggccggc
     1261 ctgggcttaa gtttttctgg ataagggggt acacactttt taggggtata ccagttaacc
     1321 gggctatctc ccggggagtt tttccctgtg agagtagttc atctatcttt ttaacttctt
     1381 catcaccata tttagctggt cttcccctcc ttttttctgg ctcaaccacc acacccagat
     1441 tttcaagtgc acccagatag cgagatgagg tgcgtgcata caagctgggt ggacatttaa
     1501 tcttcaccag atccgggtgc tgctctagaa gctccataat cctctggata gtcagtggct
     1561 gggatacgta tatttcctcc accatactca atcccttcgt agatctacaa gataccttaa
     1621 acaggttgac tccagattca tccctggacc agtttcagaa acttctgggc cttgggactg
     1681 cctggtttct ggagtgtttc cagcctgact ttctgttttt tcacctgtgc tggtgttact
     1741 ttgaagatct gggccgctct ggagatggga acctgtccct ttatatttaa aattgccgcc
     1801 cccatggctg gatccatttt gatgtgtaag ccctgggttt ttttttccaa ggaaaatttg
     1861 aaggaaatga tcttatccat ctccagcagg gattcaatat cttcatcctg aaagtccttt
     1921 agcatggtaa ctgtctggga ggggaattcc tggaatgcct tcaactggtt aatatccagt
     1981 gatggcctta gataggggaa gggcccggag tacttactgc gccggtcgta ggaagtggat
     2041 aaatagtaac gcatcaggtc ccagagtatc agggcccggg ggttttcctg gaacacgtcc
     2101 ttctgtagct tatcccttag ctgcagatcc tctttcaagc cgggattcat ctggccttca
     2161 ccatccacca gtccttcctc ctggagtatc tcccttaaaa gatcttcctt gaggtttaga
     2221 ttatcatccc actccttctg aattttggcc ctttcctccg cctttttgag agaggcatcg
     2281 tagatcaata aattagattc attttttagt ttttgctgca gcagtggggg tagtagtgat
     2341 tctcccagat cctcggagta ccccacatat cccagggcca gggcggtttg aacgtgcttg
     2401 tcattaatga ttgcaggtct tttacggtga aactgtatga actcgatacg ggcattgttg
     2461 ccatagcttt cccggatcct tttctcataa ttcaactggt cctgggcatc aattttaact
     2521 cgtttacgaa tccaccgacc atcttccatc accttaacta ccatggaaat ggttttcaca
     2581 attccccttt tttcctgttt caggatacgc accaaacccc tgaaggcctc tcttcctagg
     2641 gatgatagct ctgacatttt taccatgtaa tcccctgaga tgggaaggta cttgaggatc
     2701 cccaaccggt aaaatccatc cttatttatg tctaagttaa attgggatga gccacaccca
     2761 catttagctg gatatagttt gcccagttca tgaccacggt aacgtgattg gcaggaagtg
     2821 caagtcacgg ttgcatactc ttccaggtgg cccaggatta ttctatggga ggcaatggca
     2881 gatttaaccc ggtctagaat gttcttcttg gctgcagccc tcatacggaa aaactgggag
     2941 tgacggctca catcatggag gtcatccatt ttcatctcag atgactgcac tctcccatac
     3001 ttcagggatc ggaagggggc tttgtaaccc ttaagctcca tataatcccg gagttcgtaa
     3061 aggcggtcta ggttatcctt aaactgggag taagttccca ggaaggagtg gacatctcct
     3121 accttcccat caaaaccgtg tatctgggat aggaatttct cggcacgacc aattagtagg
     3181 gattcgttca tttaattttt tcacccacct cggcgctccc agggtctaat aaattgagac
     3241 tgtcctgagt ggctaggacc ataccttctg actttattcc aaagagtttg gctggcttga
     3301 ggttgaccag cacagttacc ttctgattta taagctccgc tggctggtag ctcttggcca
     3361 gtcctgccac cacctgtata tgcctatcct taagatcaac caacaacttc aaaaggttct
     3421 cagagccttt cacagcttct gctccagtaa tcaaccctac ccgcaggtcc atgcaggcaa
     3481 agtcttcaat acttacaaga tccataggtt ctttctcctc taaattttca taaagaatct
     3541 gcttctccct ttcaataact tcatcatcta ttttagggaa caaaggtcgg gctggtgcta
     3601 acacatgtcc tgctggtaaa aattgggcag catcatccca actatactct ggatcatcca
     3661 gattcaggat agatagtata tcccctgctt tacctggaag gtaaggtaat aataggatgc
     3721 caatggcctt ggctatctgg ttacatagat acaggcaagt ggctgcctcc tgtggattgg
     3781 att
//
LOCUS       DAOQVW010000097         3775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27470_length_3775_cov_3.256720, whole genome shotgun sequence.
ACCESSION   DAOQVW010000097 DAOQVW010000000
VERSION     DAOQVW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27470_length_3775_cov_3.256720"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            727..885
                     /locus_tag="PKK85_03020"
     CDS             727..885
                     /locus_tag="PKK85_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04165 family Cys-rich peptide"
                     /protein_id="HNS25023.1"
                     /translation="MKLGKLDEQCPKCGCQDKTIQRKLDPEYHAHGSTRAFVCSECGY
                     VFKKGEDD"
     gene            970..2082
                     /locus_tag="PKK85_03025"
     CDS             970..2082
                     /locus_tag="PKK85_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04083 family peptide-modifying radical SAM
                     enzyme"
                     /protein_id="HNS25024.1"
                     /translation="MLVPTLNCPSNCSYCWGSKDNAGVMDIEIIELVVDWLKNYRDEP
                     VHFTFHGGEPLLAGYDFYKKALPMLKEGLGENEAGFSLQSNLWLLNDELAQLLRNYDV
                     AVSSSLDGPKDINELQRGEGYFDKTMAGYDIARRNNLPVNFICTFTSYSKDLVDEIFS
                     YFLECGYNLKLHPALPSLRGDNSDPWALSPEDYGELLIKLLDMYLEHMDEIKISDFDH
                     FCKSYFIRKGTVCVLADCLGDTLAVDFEGNIYPCYRFVEMPEYVMGNVRDKPTVEDLQ
                     KSEAWKVIQSFMDLVDESCKKCRFIKMCRGGCPYNALKSDEGHIKNVDPQCTAYKMIF
                     EYISKLATKEMMVNMMPSDPSLKPVKKGGIMSLMIK"
     gene            2099..2443
                     /locus_tag="PKK85_03030"
     CDS             2099..2443
                     /locus_tag="PKK85_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25025.1"
                     /translation="MPILITNIKNNLINEAIKHVQAEMVILILNKELQADIHIPHRIE
                     YLDALDPEKSAQIRDILDHALEEGEVELALEADAMGLQILESAKNYEIFKKKNILIAK
                     NGKLDTFLACLC"
     gene            2565..3269
                     /locus_tag="PKK85_03035"
     CDS             2565..3269
                     /locus_tag="PKK85_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25026.1"
                     /translation="MNCKTVSAVILMAVIGLMIVHSYAVVSSSDLEPMGRVAFVKVMN
                     PDFYPGHLHSKLLARYAKERGSKCALITHFGGSSNYRSYMEEDVYIIELAFIDTQGTG
                     ATGPTNYTDSLKIALFGAPDGRYKYKSDGIVFNSYEEAMEHVKQVARENGQEGPLPIA
                     WHGNARNGNPIFVQGCGFPLYFDILRREYGLIPAYVYIIKGMIFPYIYSPYRNFELGN
                     FIELQLLYSGGDLDYR"
     gene            3377..3538
                     /locus_tag="PKK85_03040"
     CDS             3377..3538
                     /locus_tag="PKK85_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25027.1"
                     /translation="MIVKEWCMYCGECAGVCPRNLIEVRELSLRFKEEGCKDCQICIQ
                     ICPVNALKK"
     gene            3553..>3775
                     /locus_tag="PKK85_03045"
     CDS             3553..>3775
                     /locus_tag="PKK85_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019268492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS25028.1"
                     /translation="MLVVDILVIGAGPAGSSAAKHAALNGAEVLIIDKKSEIGAPKRC
                     AEGVSKEGLSELGIEPNPRWITKELSGVRM"
BASE COUNT         1169 a          712 c          794 g         1100 t
ORIGIN      
        1 agggaagtta tatttctgga atctttttca aactttttat ttcaattcca gaaatataac
       61 ttcccttcac accaggaaca tccaataaat tctcattttt ataaatcatg ttttcacaat
      121 tctatttcat ttttcactgc aaatcatttc atctaaaaaa ccagggaaaa acgggatttt
      181 tgtattgaga atgatggatt ggtaactgaa cgcttcaaaa aatgttgtat cctcccttca
      241 taccctgtca ttcaaaatta atgttcataa tgtattcatt ctccagttca aaagtttcta
      301 atcccaatca ttttgcatgg cgttattcac aactggctaa ctgtatattc atggtttcag
      361 ctataatttc ctgattaggt ttggatgctg tttcctaggt tatacttagg ctttccccac
      421 aatttggaaa aaattttaca tcacttacta cagtttcaca gttggggcag aatttgcaac
      481 acagctatca tattattttt ctgttagtag acaattatca atggtgacta ataaaagatt
      541 atatctattg atctaagctt ctggtaaaga gaacgatatg attatggaca cactgggact
      601 tattaacagg gccaattgcc tcaagatttt tatggtagat ctggttgaaa gtagaaaaat
      661 tgaggggtaa gttattatag caactagcct aagattaaga gtagaaacca tgtggaggca
      721 ccccccatga aactaggtaa acttgatgaa caatgtccca aatgtggctg tcaggacaaa
      781 acgatccagc gaaaactgga tcccgaatat cacgcccatg gatccaccag ggcttttgtc
      841 tgtagcgaat gtggttacgt ctttaaaaaa ggcgaagatg attagtgcag attaaagtta
      901 ttttccattg ctttccacta ggtatcttcc ccaatcccta aaatggagtc ataaaaaatg
      961 taccatgtta tgttagtacc aaccttgaac tgtccttcca attgcagcta ctgctggggt
     1021 tccaaggata atgcaggcgt gatggatatt gaaattatcg aattagtggt ggactggctc
     1081 aaaaattacc gggacgaacc tgtgcacttt acttttcatg gtggagaacc cctgctggca
     1141 ggatatgatt tctataaaaa agcccttccc atgttaaaag aagggcttgg agaaaatgaa
     1201 gctggtttct cactacaaag taatctttgg cttttaaacg atgaactagc acaactgctg
     1261 cgaaattatg atgtggcagt gagttccagt ctggatggac ctaaggatat taacgaactg
     1321 caacggggag agggatattt cgataaaacc atggcgggat atgatattgc ccgtagaaat
     1381 aatttaccag tgaatttcat ttgtaccttc acttcttatt ccaaggactt ggtggatgag
     1441 atattcagtt attttttaga atgcggatac aacctcaagt tacacccggc ccttccatca
     1501 ttaaggggtg ataactcaga tccctgggct ctttcccctg aagattatgg ggaactctta
     1561 ataaaacttt tagacatgta cctggagcat atggatgaga ttaaaatcag cgacttcgac
     1621 cacttctgta agagctactt cataagaaag ggtacggtct gtgtactggc agactgcttg
     1681 ggcgatactc tggctgttga ttttgagggg aacatctatc cctgctaccg cttcgtggag
     1741 atgccggagt atgtgatggg taacgtgcgt gacaaaccca ctgtggaaga tctgcaaaaa
     1801 tcagaggcct ggaaggtcat ccagagtttc atggacttgg ttgatgagag ttgtaagaag
     1861 tgtcgtttca ttaagatgtg tcgtggtggc tgtccctata atgcattaaa atcagatgaa
     1921 ggtcatataa agaacgttga cccccaatgc acggcttaca agatgatatt tgaatatatc
     1981 agcaagctag ccaccaaaga gatgatggtt aatatgatgc catctgatcc atccctaaaa
     2041 cccgtgaaaa aaggcggaat aatgtctctc atgatcaaat agtacgttag gagtatttat
     2101 gcccatatta atcactaata tcaaaaataa tcttataaac gaggcaataa agcatgttca
     2161 agctgaaatg gttattttaa ttttaaataa agaactccaa gcggatattc atatccccca
     2221 tcgtatcgag tacctggatg ctcttgatcc agagaagtca gcccagataa gggacatatt
     2281 agatcatgcc ttagaggaag gggaagtgga actagccctg gaagctgatg ctatgggttt
     2341 gcaaattctg gaatcagcta aaaattatga aatatttaaa aaaaagaaca ttttaattgc
     2401 taaaaatgga aaattagata cattcctggc ctgcctatgt tagaatgctg gttggaacat
     2461 gcacttttaa agctcaaaaa tgatttaaac attcatttta actatttttg gggcatataa
     2521 tgttcttaca acaaacttta ataaatcaaa acagagttta gataatgaac tgcaaaactg
     2581 tcagcgcagt tatcctgatg gctgtaatag gactcatgat agtacattcc tatgctgtgg
     2641 tttccagcag tgatcttgaa cccatgggac gtgtagcctt cgtaaaggtg atgaaccctg
     2701 atttttaccc tggacatctt cattcaaagc tcttagcacg atacgctaag gaaaggggtt
     2761 caaaatgtgc tttaataacc catttcggtg gaagttctaa ttatcgtagt tacatggaag
     2821 aagatgttta catcattgaa ctggccttca tagataccca gggaactggt gccacagggc
     2881 caacaaacta cactgattct ttaaaaatag cgcttttcgg tgccccagat gggcgttaca
     2941 aatataaatc cgatggaatt gttttcaaca gctacgaaga ggctatggaa catgttaagc
     3001 aagtcgcccg ggaaaacgga caggaaggac ctctacccat agcttggcat ggtaacgctc
     3061 gtaatggaaa cccaatattt gtacagggtt gtggttttcc actgtatttt gatattctcc
     3121 ggagagaata tggtttaatt cctgcctacg tctacatcat taaagggatg atctttccct
     3181 atatttacag cccctaccga aacttcgagt tgggaaactt cattgaactc caactactct
     3241 acagtggagg agatctggat tacagataac gttcaccccc ttaaattcgg gttgttaaac
     3301 ccaattttat aaatatccca gtcattaaat actaagataa ccaaattact tgtctaactt
     3361 ttaaaggatg atatctatga ttgttaagga atggtgtatg tactgcgggg agtgtgctgg
     3421 agtatgccct cgcaacctca tcgaagtaag agaattaagt ctaagattta aggaggaagg
     3481 atgcaaagat tgtcagatct gcatccagat atgtcctgtg aacgccttaa aaaaataagg
     3541 gagtgctttt taatgttagt agttgatatc ctggtaattg gagccggtcc agccggctcg
     3601 tctgcagcta aacacgctgc tttaaacgga gcggaagtgc tcatcatcga taaaaaatcg
     3661 gaaatagggg cccccaaacg ttgcgctgag ggggtgtcca aagaaggcct cagcgaactg
     3721 ggcattgaac caaacccccg ctggataacc aaagaactta gtggggtgcg tatgg
//
LOCUS       DAOQVW010000098         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27496_length_3773_cov_2.757127, whole genome shotgun sequence.
ACCESSION   DAOQVW010000098 DAOQVW010000000
VERSION     DAOQVW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27496_length_3773_cov_2.757127"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKK85_03050"
     CDS             <1..469
                     /locus_tag="PKK85_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNS25029.1"
                     /translation="GGRFTGEPAYFKHVLSAAKGLFGKMGTDASDYDHVVFHQPNGKF
                     YIRAARKLGFKEDQYKTGLLTPYIGNTYSGATPLGLAAVLDIAQAGERIMAVSYGSGA
                     GSDAFSITVNDEIEQKRDLAPKVQELVDNKCYVDYAVYAKFKGKLRMAGLTQH"
     gene            497..1648
                     /locus_tag="PKK85_03055"
     CDS             497..1648
                     /locus_tag="PKK85_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNS25030.1"
                     /translation="MRDVAIIGVSQTKFGELWDISFRDLISEAGLKAIEDANIEGADL
                     EAMFVGNMSAGLFVQQEHIASLIVDHTGLTPLPCVRVEAACASGGLALRNGIMAVASG
                     YHDVVISAGVEKMTDVVDPTPAIATASDQEWEAQQGVTFPSLYAMMARRHMYEYGTTR
                     EQLAMMSVVNHKNGALNPLAQFPMEITVDQVLNSSLVADPLRLLDCSPVTDGAAAVIL
                     CPAEDARKYTDTPVYVKASAQASGTIALHDRRDITTIDATVHAANTAYKMANMEPEDI
                     QAVEVHDCFSINGLLAIEDLGFVEKGQGGPAIEDGMTERDGRIPVNPSGGLKSRGHPL
                     GATGIAQTAEIVWQLRGEAGKRQIDGIEVGMTHNIGGTGGTATVHIFSN"
     gene            complement(1726..2055)
                     /locus_tag="PKK85_03060"
     CDS             complement(1726..2055)
                     /locus_tag="PKK85_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNS25031.1"
                     /translation="MAIEDLEPQKCLELIKKNENNPNFIILDVRGPKEHAAGHVENSV
                     LLEVRSPDFKEKLEELDKDKKYLVYCKSGMRSKKATKIMEEMGFLNLCTMKKGFKNWV
                     DNGLPQE"
     gene            complement(2240..>3773)
                     /gene="thsA"
                     /locus_tag="PKK85_03065"
     CDS             complement(2240..>3773)
                     /gene="thsA"
                     /locus_tag="PKK85_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HNS25032.1"
                     /translation="ILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVVTNDGVTILKEM
                     DIEHPAAKMLVEVAKTQEDEVGDGTTTAVIIAGELLKKAENLLDQDIHPTIVAMGYRQ
                     AAEKAQEILNVIAIDADDRETLLKVAMTAMTGKGTEKAREPLADLVVGAVKQVEENGE
                     IDKNHIKLEKKDGATIDDSKLVNGVIIDKERVHPGMPKKVEDAKIALLNSAIEVKETE
                     VDAEIRITDPAQMQAFIEQEEQMIRDMVAKIEDAGANVLFCQKGIDDLAQHYLAKSGI
                     MAVRRVKKSDMEKLARATGATVVTNIEDLSYDDMGDAGSVAEKRISGEEMIFVEECKD
                     PKSVTLLIRGSTEHVVDEIERAVEDAIGVVAATVEDGKVVAGGGAAEISVAKGLKEYA
                     ETISGREQLAVSAFAEALEVVPKTLAENAGQDSIDALVDLRAAHEKSLYMGLDVFKGE
                     VTDMYRAGVIEPHRVKKQAIQSAAEAAEMILRIDDVIASSGSKEPDMGDMGGMGGMPP
                     MM"
BASE COUNT          893 a          920 c          874 g         1086 t
ORIGIN      
        1 cggaggacga ttcacaggtg aaccagccta cttcaaacac gtgttatcgg cggctaaggg
       61 actgtttggt aaaatgggta ccgatgcatc tgattatgat catgttgttt tccaccagcc
      121 caatgggaaa ttctatatcc gggccgcccg taagctgggc tttaaagagg atcagtacaa
      181 gactggactt ttaactccct acattggtaa cacatactct ggtgcaactc ccctgggact
      241 agccgctgta ctggacattg ctcaagcagg ggaacggatt atggcagttt cctatggctc
      301 cggagccggt agcgatgcct tcagcatcac ggtgaatgat gaaattgagc aaaaacggga
      361 ccttgctcct aaggtacagg agttggtgga taataagtgt tacgtggact acgctgttta
      421 tgccaagttc aagggaaaat tgaggatggc aggattaaca cagcattaaa tataagattt
      481 tatggaggaa tcatagttga gagatgttgc cattatcggg gtttctcaga ccaagtttgg
      541 ggaactctgg gacatttcat ttcgggatct aatttccgag gccggattaa aggccattga
      601 agatgcaaat attgaaggag ccgatttaga agccatgttc gtggggaaca tgtctgctgg
      661 tttattcgtc cagcaggaac atatagcctc cctaatcgta gaccataccg gtttaacacc
      721 attaccctgt gtcagggtgg aggcggcttg tgcctcagga ggtctagctc tccggaatgg
      781 tataatggcc gtggcctctg gctaccatga cgtggtcata tctgccggtg tggaaaaaat
      841 gacagacgtg gtggatccta ctccagcaat tgccactgcc tctgaccagg aatgggaagc
      901 tcaacaggga gttactttcc cttctttgta tgccatgatg gcccgcaggc acatgtacga
      961 gtatggaacc accagggagc agctggccat gatgtccgta gtcaaccata aaaatggggc
     1021 tttaaatccc ctggcccagt tccctatgga aattaccgta gaccaagtct taaattccag
     1081 cttggtagct gatcccctgc gacttctgga ctgctctccc gttactgacg gagctgcggc
     1141 agttatactc tgtcccgccg aagatgcccg taagtacacc gacacacccg tatacgttaa
     1201 ggcctcagcc caggcctcgg gaaccattgc cttacacgat cgccgggata tcaccaccat
     1261 cgatgccaca gtacatgcag ccaatactgc ctataagatg gccaatatgg aaccagagga
     1321 tatacaagcc gtggaggttc atgattgctt cagtatcaac ggactcctgg ctattgaaga
     1381 cctgggcttc gtggaaaaag gccaaggtgg accagccatc gaagatggga tgactgaacg
     1441 agatggccgt ataccagtca acccctcagg cgggctaaaa tcacgtggtc acccactggg
     1501 tgctactggt atcgcgcaaa ctgcagagat tgtctggcaa ctccgtggtg aagctggtaa
     1561 acgacaaatt gatggcatag aagttggtat gacccacaac attggtggca ccggtggtac
     1621 cgccacggtt catatatttt ccaactagtc tgttaatata aattaaaatg aggaatgggg
     1681 gaataagatt ccccaaactt tctattttta atggattcta tgggactact cttggggcaa
     1741 tccattatct acccaatttt taaagccttt tttcatggtg caaagattca aaaatcccat
     1801 ctcctccatg atcttggtgg cttttttact tctcatccct gacttgcagt atacaaggta
     1861 ctttttatcc ttatcaagtt cctctaattt ttccttaaaa tcgggggatc tgacctctaa
     1921 aagcactgaa ttttcaacat gacctgcagc atgttctttt gggcctctaa catccagaat
     1981 aataaagtta gggttatttt cgttcttttt aattaattct aaacattttt gaggttctaa
     2041 atcttcaatc gccatgttat cactcctaaa ttcttatatt gattaatttt gattgtacag
     2101 agtttagtgg aggttggtga aataacttgt tgttcattgg attcaaaaaa aacctaccta
     2161 acatggtaca ctgataataa aaatcttact tggtgctgaa ttaatgacta aaaaaaaaat
     2221 cgaggtaagt ccgggggact tacatcatag gaggcattcc acccattcca cccatgtcac
     2281 ccatgtctgg ttccttagat ccagaagagg ctatgacatc gtcgatcctt aggatcatct
     2341 cggcggcttc agcggcggat tgtatggcct gttttttgac ccggtgtggt tcaattacac
     2401 cagcccggta catgtcggtg acttctcctt tgaagacatc cagccccatg tacagggatt
     2461 tttcgtgggc agctcgcagg tccaccaaag cgtcaatgct gtcctgtcct gcgttttcag
     2521 ccagggtttt aggtacaact tccagggctt ctgcaaaggc agatacagcc aactgttccc
     2581 ttccacttat ggtttctgca tactctttca gacctttggc cacagatatt tcagctgctc
     2641 ctccaccagc tactacttta ccgtcttcta cagtggcagc aactactccg atggcatctt
     2701 ccactgctct ttcaatttcg tccaccacgt gttcagtgga tccgcggatg agtagggtta
     2761 cagacttagg atctttacac tcttccacga agatcatctc ttcaccggag atcctcttct
     2821 cagcaacaga cccggcatca cccatgtcat cgtaggacag atcttcgatg ttggtcacca
     2881 cagtggctcc ggttgcgcgg gccagttttt ccatgtcgga ttttttaacc cggcgaacgg
     2941 ccatgatacc ggatttggcc aggtagtgct gggccaggtc atcaatacct ttctgacaga
     3001 atagaacgtt ggctccggcg tcttctattt tggctaccat gtcccggatc atctgttctt
     3061 cctgttctat gaaggcttgc atctgggctg ggtcggtgat tctgatctca gcgtccactt
     3121 cggtttcttt aacttcgatg gcactgttta gaagtgcaat tttggcgtct tccacctttt
     3181 taggcatgcc agggtgtaca cgttccttgt caatgattac cccgttgacc agtttggagt
     3241 cgtctatggt ggctccgtct ttcttttcaa gttttatgtg gtttttgtcg atttcaccgt
     3301 tttcttccac ttgtttaacg gctcccacca ccaaatccgc caggggttcc cgtgctttct
     3361 cggttccttt tccagtcatg gcagtcatgg ccactttcag gagggtttct cggtcatcag
     3421 cgtcgatggc gatgacattt aatatttcct gggctttttc agctgcttgt cggtatccca
     3481 tggccacgat ggtggggtgg atgtcctgat ccaacaggtt ttcggccttt ttcaatagtt
     3541 caccagcaat gataactgca gtggtagttc cgtcccctac ttcatcttcc tgggtcttgg
     3601 ctacttccac cagcatcttg gcggcagggt gttcaatgtc catctctttg aggatggtca
     3661 ctccatcgtt ggtcaccacg atgtcaccta atccgtcaac cagcatttta tccataccct
     3721 tggggcctag agtggtcctt acagtttcag ccagtacttt tccagctaga ata
//
LOCUS       DAOQVW010000099         3737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27862_length_3737_cov_3.638783, whole genome shotgun sequence.
ACCESSION   DAOQVW010000099 DAOQVW010000000
VERSION     DAOQVW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27862_length_3737_cov_3.638783"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="thrC"
                     /locus_tag="PKK85_03070"
     CDS             complement(<1..1080)
                     /gene="thrC"
                     /locus_tag="PKK85_03070"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNS25033.1"
                     /translation="MMICISCGAEYDNQDIIYTCQKCGSILEVICQPRVSRDVFECRR
                     STMWKYKEFMPVDHSLIVSLEEGGTPFCRCDKLGDDLGVELYVKVEGSNPTGSFKDRG
                     MSVGITKAMELGVDTVGCASTGNTSASLAAYAARAGLRCVVLLPAGKVALGKLAQAMF
                     HGAEVISVRGNFDEALEAVTALALEGQLYLLNSVNPFRLEGQKSIGFEIVDDLGWKSP
                     DRIILPVGNAGNISAIWKGITEFHEAGFIKDLPRMTGIQAEGAAPIVQAVFQGAEDIV
                     PVEDPETVATAIRIGAPVSAKKALRAIYESDGMAETVTDAEILDAQKLLARREGIGVE
                     PASAASIAGLKKLVESGQINKGEQIV"
     gene            complement(1104..1784)
                     /locus_tag="PKK85_03075"
     CDS             complement(1104..1784)
                     /locus_tag="PKK85_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNS25034.1"
                     /translation="MARDKYESYIGGLYRRNERFLIISTVIFFASMFIGYALSGMLAW
                     IMGGVLDNMKRQVGQGELKLTTLSIFLNNFKVILYLYGGGLVLGIITTYLLFYNGMFI
                     GYAASQYPLSNFIVYTIPHGIFEIVGIIVSGAAGFKLAGVVVDIIKGLRHIQPEMSRS
                     NQFNYLLKVYYDDFKESLVLLSIAVILLLIGAFIEANFTLTWANYVTGVTTQSVSENP
                     LNSLFRVS"
     gene            complement(1819..2913)
                     /locus_tag="PKK85_03080"
     CDS             complement(1819..2913)
                     /locus_tag="PKK85_03080"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNS25035.1"
                     /translation="MIDPWSSALVDYEKLIQQFGIKPFQDVLGDIEDPSLLMRRGIIF
                     GHRDFGRIVKALQEKTPFAVVTGMMPSGRMHIGHKMIVDQIIWYQKMGAEVYIPIADM
                     EAYSARGVEFEESRKIALEEYITNYIALGLDFSSKNITTYLQSENQDVNHLAYQLGRR
                     VNWNEMKAIYGFNGSTNLSHLYVPLIQVADILHPQLESYGGPKPTVVPVGPDQDPHIR
                     LTRDLAERFKNQFHFVTPSSTYHRFITGLTGGKMSSSKAKTAIFLSDSPEEAEKKLKT
                     AKTGGRESREEQEKLGGVPSECVVYEMLLYHLVDDDQELAEIYKQCIQGQIQCGECKM
                     QAAVKVRELFEQLERKREQARDEALKVLEG"
     gene            complement(2974..3477)
                     /gene="endA"
                     /locus_tag="PKK85_03085"
     CDS             complement(2974..3477)
                     /gene="endA"
                     /locus_tag="PKK85_03085"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HNS25036.1"
                     /translation="MNGELKGEMVTVTSPKAIRLHENSHFGQLVKDELQLPLVEALFL
                     VEKDKLTLSSKGSDLSWEEFADLLRDEGLFTKYLVYRDLRNRGYIVKTGFKYGSEFRL
                     YERGRSPGNGHSDFLVKILTEDQHININDFSSYVRVAHGVNKKLMLAVVDDEQDITYY
                     NVEWTRP"
     gene            complement(3517..>3737)
                     /locus_tag="PKK85_03090"
     CDS             complement(3517..>3737)
                     /locus_tag="PKK85_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HNS25037.1"
                     /translation="CLDVLVKMDSKSKVPWKHPTSHTLKGNYDDMTPLGTLFEDVTHL
                     FLDGLIAEFMARMGQKETDLRRRHATLE"
BASE COUNT          871 a         1002 c          868 g          996 t
ORIGIN      
        1 gacgatctgt tcccccttat ttatctgccc cgactcaacc aacttcttaa gaccagcgat
       61 ggatgctgcg gaggcgggtt ccaccccgat cccctcccgt cgggctagga gtttctgggc
      121 atccaggatc tctgcatcgg tgacggtctc cgccatacca tccgattcgt atatggcccg
      181 cagagctttt ttggcactta ctggagcccc gatacgtatg gctgtggcca cggtctccgg
      241 gtcttccact ggcacaatat cctctgctcc ctgaaaaact gcctggacta tgggagcagc
      301 gccctctgcc tggatacccg tcatacgggg caggtccttt atgaaaccgg cctcatgaaa
      361 ttcggtgata cccttccaga tggcggatat gttcccggcg ttacccacgg gtaagatgat
      421 ccgatccggt gatttccagc ccagatcatc cacaatctca aatcctattg acttctgccc
      481 ctccagacgg aagggattaa cggaatttaa tagataaagc tgcccctcca gggccagggc
      541 agtcacggcc tccagggcct catcgaagtt gccccttact gatataacct cggcgccatg
      601 gaacatggcc tgggccaact tccccagggc caccttaccg gcaggtaaaa gcacaacaca
      661 ccttaaaccc gcccgggctg cgtatgctgc aagagaggcc gaggtattac ctgtggaggc
      721 acagcccact gtgtccacgc ctaactccat ggccttggtt atgcccacac tcatccccct
      781 atctttgaag ctgccggtgg ggttggatcc ctccaccttt acgtagagct ccacccccag
      841 atcatctccc agcttatcac agcggcagaa aggtgttcct ccctcctcta ggctgacgat
      901 gagggaatga tccacaggca tgaattcctt gtacttccac atggtggatc ttcgacactc
      961 gaaaacgtcc cgggataccc ggggctggca tataacctcc agtatggatc cgcatttctg
     1021 gcaggtgtag attatatcct ggttgtcata ctcggcacca caggatatgc atatcatcat
     1081 tataaatcac ctttttttta gttttaagat actcggaaga ggctgtttaa aggattttca
     1141 ctgacacttt gggtggtgac tccggtgacg tagttagccc aggtaagggt gaagttagcc
     1201 tctatgaagg ctccgatcag taacaggatc acggcgatgg acaggagtac cagcgattct
     1261 ttaaaatcat cgtagtaaac ttttaaaagg tagttgaact ggttggatcg ggacatttcc
     1321 ggttgtatgt gtcgcagtcc ctttattata tctacgacca caccggccag tttaaaacca
     1381 gctgcaccag atactatgat tcccacaatt tcgaagatac catgggggat ggtgtataca
     1441 atgaaattgc tcaggggata ctgggatgca gcgtagccta tgaacatgcc gttgtagaac
     1501 agtaagtagg tggtgataat gcccaagacc agtcctcccc cgtagaggta gagaatcact
     1561 ttaaagttgt ttaaaaatat ggagagggtg gtgagtttta gttcgccctg acctacctgt
     1621 cttttcatgt tgtccaggac accacccatg atccacgcca gcatcccgga cagggcgtat
     1681 ccaatgaaca tggatgcaaa gaatatgacg gtggaaataa tcaaaaagcg ctcattccta
     1741 cggtacaatc ccccaatata actttcatat ttatcccttg ccatttcaac ctcctcccat
     1801 gatagactaa cgtagtattt atccctccag cactttaagt gcttcatccc gggcctgctc
     1861 ccgcttacgt tccagttgtt caaataactc acggacctta acagcggctt gcatcttaca
     1921 ctcgccacac tggatctggc cctggatgca ctgcttgtaa atttcggcca gttcctggtc
     1981 atcatccacc aggtggtaga gcagcatctc gtagaccaca cactcactgg gaactcctcc
     2041 cagtttttcc tgctcctccc gactctcccg gccaccggtc ttggctgttt ttaacttttt
     2101 ctctgcctct tctggactgt ctgacaggaa tatggctgtt tttgctttgc tactggacat
     2161 ttttccaccg gtgagtccgg tgataaaacg atggtaagtg gaggagggag taacgaagtg
     2221 gaactggttc ttgaatcgct cggcaagatc ccgggtgagg cgtatgtggg ggtcctgatc
     2281 cggccccact ggaaccactg tgggtttagg gccgccatag gactccagtt gtggatgcag
     2341 tatgtctgcc acttgtatca ggggtacgta aaggtgagat aggttggttg acccgttaaa
     2401 accatagatg gccttcatct cattccagtt caccctgcgg cccaactggt aggccaggtg
     2461 gttcacatcc tgattttcag actgcaggta ggtggtgatg ttttttgaag aaaaatccag
     2521 tcccagggcg atgtagttgg tgatgtactc ctccagggct atcttacggg actcctcaaa
     2581 ctccactccc cgggcggagt aggcctccat atcagcgatg ggaatgtata cctcggcccc
     2641 catcttctgg taccagatga tctgatccac gatcatcttg tggccgatgt gcatcctgcc
     2701 actgggcatc atccctgtta ccacggcaaa gggagttttc tcctgcaggg ccttaactat
     2761 acggccaaaa tccctgtgac cgaatataat accccgcctc atcaggaggg agggatcctc
     2821 tatatctccc agcacatcct ggaagggctt aattccaaat tgctggatca gtttttcgta
     2881 gtcgactagg gctgaactcc aggggtctat cagattcatc accttgatgt taatattaga
     2941 gcttatggta gggatataat ataactatct cccctagggc ctggtccatt ccacgttgta
     3001 gtaggtgatg tcttgttcat catcaaccac tgccagcatc agcttcttgt taacaccatg
     3061 ggctacccgg acgtaactgg agaaatcgtt gatatttatg tgctgatcct cggtgagaat
     3121 tttaaccaga aagtcggaat gcccattacc gggagatctt cccctttcat agagcctgaa
     3181 ctctgatcca tacttgaaac cggtttttac aatgtaacct ctgtttctaa ggtccctata
     3241 aaccaggtac ttggtgaaaa gtccttcatc ccttaaaagg tcggcgaact cctcccagga
     3301 tagatcactt cccttactgg acaaagtcaa cttatccttt tccaccagaa acagtgcttc
     3361 tactagtggt aattgtaact cgtccttgac caactgtcca aaatggctat tttcatgtaa
     3421 tcggatggct ttcgggctag ttaccgttac catctcccct tttagttccc cattcatatc
     3481 ctttcaccgg actgatactc catcagaagg gaaattctac tccaaggtag cgtgtcttct
     3541 acgcagatca gtttctttct gtcccatacg ggccatgaac tcggctataa gaccgtccag
     3601 gaataggtgg gtgacgtctt caaagagggt gcccaggggt gtcatgtcat cgtagttacc
     3661 cttcaatgta tgggaggtgg gatgtttcca gggaactttg ctcttactat ccatcttcac
     3721 cagcacatcc aggcatt
//
LOCUS       DAOQVW010000100         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27889_length_3735_cov_3.935870, whole genome shotgun sequence.
ACCESSION   DAOQVW010000100 DAOQVW010000000
VERSION     DAOQVW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27889_length_3735_cov_3.935870"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..943)
                     /gene="fhcD"
                     /locus_tag="PKK85_03095"
     CDS             complement(44..943)
                     /gene="fhcD"
                     /locus_tag="PKK85_03095"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HNS25038.1"
                     /translation="MHINNVEIEDTFAEAFDIYVSKFLVTAATQKFAKIAADETVGFG
                     SSVISCPAEAGIDSYVPPNETPDGRPGYVVMICHPKKEELEHQLIERIGQCILTSATT
                     AVFDAMGDLADEKLRIGFKLKFFADGYDSEGVIGGRKIYKIPLMSGDFVIEEELGIKG
                     GVAGGNFFVMAENQASALLGAEVAVDAIQGVPGTITPFPGGIVASGSKVGSLKYKFMH
                     ATTNEKFCPTLRGAEGIDCEIPEGIEGIYEIVIDGVSEEAIKEAMKAGITAAVKVPGV
                     KRIYAGNYGGTLGAYQIWLTSLF"
     gene            complement(959..1351)
                     /locus_tag="PKK85_03100"
     CDS             complement(959..1351)
                     /locus_tag="PKK85_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNS25039.1"
                     /translation="MMNAEMMEIIEKESAFVATATPEGVPNVVPIGFIKPLDAKTVII
                     ADSYMVKSRANLEANPKISFVVQDAARYPYQFKGSVKIFTEGKYFDEVVRWVKDTSPL
                     APSPKAAIVITIEEIYSVKVGDAGKKLA"
     gene            complement(1589..2077)
                     /locus_tag="PKK85_03105"
     CDS             complement(1589..2077)
                     /locus_tag="PKK85_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS25040.1"
                     /translation="MMLIGIMSDSHDHIEAIRMVVNFFNQKKVDLVLHAGDLISPFTA
                     SEFKQLKMPFEAIYGNNDGEREGLKTAFQELCDLEDFKEIDVDGRKIAVIHGTNQKLV
                     DALANGGTYDVLIRGHSHKFHVSCDKTMIINPGETCGYLSGKKTVILLSTEDLKFKKV
                     IL"
     gene            2444..3118
                     /locus_tag="PKK85_03110"
     CDS             2444..3118
                     /locus_tag="PKK85_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNS25041.1"
                     /translation="MRNPGLRLIIPLVDRMVKPSLQIVTMPIPAQKIITQDNVTIDVA
                     AVAYFKVVDAYKAVVEIENYNRAVNQISQTTVRSVVGQFTLDEVLSETPLVNSKIQEI
                     IDTHSEAWGIKVTTVEIKDIKLPDSMQRAIALQAEAEREKRAKIISAEGEYLAAGKLG
                     EAADIIAEHPVALQLRIMQVLSIIAAEKNSTIVFPAQLLNSIRDIKDFMESEVPSAQY
                     IKKKSE"
     gene            3305..>3735
                     /locus_tag="PKK85_03115"
     CDS             3305..>3735
                     /locus_tag="PKK85_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNS25042.1"
                     /translation="MIGQLRRRDLLLIEHPLGMIMQGIGVVVLSPVIFALIYNENTYL
                     AFIIPSILSLAVGHLLKMSSSQNNNLGIKHGMIIAAVAWLWAAFIGSLCLVMGTNVDF
                     LNAFFESMSAWTGSGLSIFINVEVLPKSILFLRSLEQWIGG"
BASE COUNT         1078 a          770 c          712 g         1175 t
ORIGIN      
        1 gtagataaaa aaaagtttaa aaagaaaaat agaagatttt ttcttaaaat aagctggtca
       61 gccagatctg ataggctccc agggttccac catagttacc tgcgtagatc cttttaacac
      121 caggaacctt taccgccgcg gttatccctg ctttcatggc ctcctttatt gcttcctcac
      181 tgactccatc aataacgatt tcatagatac cttctattcc ttcagggatc tcacagtcta
      241 taccctctgc acctctcagg gtggggcaga acttttcatt ggtggtggca tgcatgaatt
      301 tgtactttag tgaacccact ttggaacccg atgccactat acccccgggg aaaggagtta
      361 tagtaccagg aaccccctgg atagcgtcaa ctgcaacttc agcacccaga agggcagatg
      421 cttgattttc agccatgaca aagaaattgc caccagctac tcctcctttg attcctaatt
      481 cttcctctat tacaaaatct cctgacatga gggggatttt ataaattttt cttccaccaa
      541 tcactccctc agaatcatag ccatcagcaa agaatttgag tttaaatcct attctcaact
      601 tttcatcggc caggtccccc atggcatcaa atactgcggt ggtggctgaa gttaatatgc
      661 attggccgat tctttcaatg agttgatgct ctagttcttc ctttttaggg tggcagatca
      721 ttaccacgta acctggcctc ccatcaggtg tttcgtttgg agggacgtag gaatctatcc
      781 cggcctcggc tggacaggaa attacggacg aaccaaaccc taccgtttca tcggctgcaa
      841 ttttggcgaa tttttgggtg gcagctgtaa ccaggaattt tgagacgtaa atatcaaagg
      901 cttctgcaaa tgtatcttct atttccacgt tgttaatatg catcaaaatc accatgtttt
      961 aagctagttt tttaccggcg tcaccgactt taactgagta aatctcttct atggttatta
     1021 ctatagctgc ttttggacta ggggctagag gactagtgtc tttaacccac ctaaccactt
     1081 catcaaaata tttgccttcc gtgaatattt tcacagaacc cttaaactgg tatgggtagc
     1141 gggccgcgtc ctgaaccacg aaggatattt taggattggc ttccagatta gccctgcttt
     1201 ttaccatgta gctatccgca attatgaccg tttttgcatc caatggtttg ataaatccta
     1261 tgggtaccac gttcggaact ccctctggag tggcagtggc aacaaatgcg ctctctttct
     1321 caattatttc catcatttcc gcgttcatca tttaatagac ctccatttcc aaattcagac
     1381 atggtgtgtt taaatagcaa tagatattga ttgatttaca tacttttata atctttttga
     1441 tttaattttg tgaaatgaca gtcctgtttt ctacctattc atagagatct ggagtttaaa
     1501 actaatataa acaccataat tgataacaat catttatttt ctctgaaaga taaataaaca
     1561 cctgcataaa gatttttttt tcaattaact ataaaattac ctttttaaac tttaaatcct
     1621 cagttgaaag gagaattact gttttcttac cggaaagata tccgcatgtt tctccaggat
     1681 tgattatcat tgtcttatca catgaaacat ggaatttatg actgtgtcct cttatgagaa
     1741 catcgtaagt acccccattt gccaaagcat ctactaattt ctgattggtc ccgtggataa
     1801 cagcgatttt ccttccatct acatctatct ccttaaagtc ctccagatca caaagttcct
     1861 gaaaagctgt tttcaagccc tctctttccc catcattgtt tccatatata gcctcaaagg
     1921 gcattttaag ttgtttaaat tctgaggcag tgaaggggga gatgagatct cctgcatgta
     1981 gaaccagatc aacttttttt tggttaaaaa aattaaccac cattctgatt gcttctatat
     2041 gatcatggct gtcggacatt attccaatta gcatcatgtg taccccgata atgatatctt
     2101 accaatggta ttctatctgt tctagcaatt atctattatg aaaaaaatta atcgtttttt
     2161 tatgccataa accataattt ccagatgttt caaatcttat gatgatagag atttccttaa
     2221 cagtaattca ttcaaaatat tagtaagcaa ttaccataat tagtatattg aatatacagt
     2281 acagagtgaa tgttgaaaat ttgttggagg ttgattgaat ggcactggat cttatatcat
     2341 taatcatcat tggtgtggtg attttaatta ttctggcatt atctaccgta tcgtcaatca
     2401 atatgaacgg ggcgttgttt ttagactagg gaaggttata ggagtgcgga acccaggact
     2461 tcgtttaatt atccccctgg tggataggat ggttaaacct tcattgcaga tagttactat
     2521 gcccattcca gcccagaaaa ttataaccca ggacaacgtg accattgatg tggcagcagt
     2581 agcctatttc aaagttgtag atgcctacaa agcagtggtg gaaattgaaa actacaaccg
     2641 ggccgtgaac caaatctctc agaccaccgt ccgtagcgtg gtgggacagt tcactttgga
     2701 tgaagtacta tccgaaactc ccctggtaaa cagtaaaatt caagagataa tcgacaccca
     2761 cagcgaagca tggggtatta aggtaaccac tgtggagatt aaagatatta agctacccga
     2821 tagcatgcaa agagccattg cattacaagc agaggcagaa agggaaaaga gagcaaaaat
     2881 catatcggcc gagggagaat atcttgctgc cggcaaattg ggagaagcgg cggatatcat
     2941 tgctgaacac cccgtagcac ttcaattacg tatcatgcaa gtgctgagca tcattgcagc
     3001 tgagaaaaac tccaccatag tattccctgc ccagttactc aacagcataa gggatattaa
     3061 ggacttcatg gaatctgaag ttcccagtgc ccagtatatt aaaaaaaaat ctgagtaaaa
     3121 tcccgtttag gtgattctca aatccacaat attatttgga taaacaaggt acctgaacaa
     3181 ttttaatcct gttaattccc cagacccccc ttaattcaca gaatatcgat tgaagtccta
     3241 gatttctggc agttaatagc aatctatatt agatttatat tctatgtcgt cgatgattgt
     3301 atagatgata ggtcaactta gaagaagaga tttgctttta attgaacatc cccttggaat
     3361 gatcatgcaa ggcattgggg tagtagttct ctcgccggta atatttgcct taatatacaa
     3421 tgaaaatacc tacctggcat ttattattcc ttctattttg tccctggctg taggtcatct
     3481 gttgaaaatg agttcatccc agaacaacaa tttagggatt aaacatggga tgattatagc
     3541 agcagtagcc tggttatggg cagcttttat tggaagttta tgtctggtga tgggtaccaa
     3601 cgtggatttt ttaaacgctt ttttcgaaag tatgtctgcc tggaccggta gtggtttgtc
     3661 tatttttatc aatgtggagg tactgcccaa gtcaattcta tttttaagaa gcctggagca
     3721 gtggattggt ggctt
//
LOCUS       DAOQVW010000101         3732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27929_length_3732_cov_3.361980, whole genome shotgun sequence.
ACCESSION   DAOQVW010000101 DAOQVW010000000
VERSION     DAOQVW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27929_length_3732_cov_3.361980"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKK85_03120"
     CDS             <1..360
                     /locus_tag="PKK85_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNS25043.1"
                     /translation="GKVPVQITIEGDEETLNRVIDVLEENGIQIMEVDGVLRREKITT
                     ILVGNIVDQDLKDTVNLLNQLPGVRVADLDLKMSDPPERSASKIVMEAREGLKQEVFK
                     KVNEIGDLKGFLVINEI"
     gene            365..1384
                     /locus_tag="PKK85_03125"
     CDS             365..1384
                     /locus_tag="PKK85_03125"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNS25044.1"
                     /translation="MILMKIVILGFGAVGQGVTRVISLKREYLLDKYGLQLDIVAAVD
                     RSGAALSENGLEPETLLETKAETGKLSQYPEYGVEGVSGLELLDEVEYDCLVEVTPTN
                     IEDGEPALSHMQKAMEKGKDVVTSNKGPLALSFTKLAETAQKTGTNFKYEASVGGAMP
                     IINLAHDTLAGSDVESVYGILNGTTNYILSRMAKEGSSYEQTLNEARELGIAETDPSQ
                     DVDGWDAACKIVILANSVLNRECALEDVKVEGISKITPEAMALAKKEGYLIKLIGEAS
                     QDVLEVSPRLVKEGSPFAVEGTLNVATLKTDLAEDVTVVGKGAGSIETASAILSDLLN
                     IWNSG"
     gene            1423..2616
                     /locus_tag="PKK85_03130"
     CDS             1423..2616
                     /locus_tag="PKK85_03130"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HNS25045.1"
                     /translation="MKYVVVIGDGMADYPLEELDGKTPLQKAKTPNMDRIAREGVSGT
                     LKTVPNGMEAGSDVANLSIMGYNPHEYYTGRGPLEAASMGARLRRDEVAFRCNLINTE
                     AGLLSDFNAGHISTPEASEIIEYLNQSFYQYGKFYLGTSYRHLFVFPRKEAADLESTP
                     PHDVVGEPLSRHKLKPEDDELACELNQLMDQSHGKLEGHPVNKKRSQMGKKTANMIWL
                     WGQGRKPQMPPFKAKYGLKGATITGVDLIKGIGVYLGLTNIKVPGATGYYDTDYCGKV
                     NYALEALEEHDLVFIHVEAPDEAGHAGDLEEKIRAIERIDHRILGKLWKELPQHHDFA
                     MAILPDHFTPLSVRTHTPEPVPYAMCKSGGVVDEVDSYHEFSVANGSQGLREGHLFLK
                     NLLEY"
     gene            2660..>3732
                     /locus_tag="PKK85_03135"
     CDS             2660..>3732
                     /locus_tag="PKK85_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNS25046.1"
                     /translation="MIQGNSLRYYTAVLAGKTASFILKKVLKGEASAMPGKVAMQIYP
                     NILQVVDQRCQKKILVTGTNGKTTTTNLLHHIIKSQGLRVLSNLRGANMPQGLVSSFL
                     NHQDAEYDWGVFEVDEGSFQEVVKHLKPDYVVVTNFFRDQLDRYGEIEKAFQDIFDAL
                     KPLDTTLILNADDPLVSSFKSLKKRNIFYGVGENQFSTLEEGVIESRFCPSCASRLEY
                     DYFNYGQLGGYHCSQCGFTNPPREYEITRVEYSDPGYHLKFKTENVKSMDLDFSYDGI
                     FNTYNCCAALSVALEIGMETGKVIESIGRFEYHLGRMETFQFPDKLVKVVLVKNPIGL
                     GEVLKSLSLDGKVKSLLMVLNDNP"
BASE COUNT         1053 a          838 c          984 g          857 t
ORIGIN      
        1 ggaaaagttc cagtccagat caccattgag ggggatgaag aaaccttaaa ccgggtaatc
       61 gatgttctag aggaaaatgg tatccagata atggaggtgg atggtgtact gcgccgggag
      121 aagatcacca ccatcctggt tggcaacatc gtggaccagg atcttaagga tacagtgaac
      181 ctcctcaacc aacttccagg ggtacgggtg gcggatctgg atcttaagat gtcggatcca
      241 cccgaaagat ctgcatccaa gattgtgatg gaagcccggg aaggacttaa acaggaagtg
      301 ttcaaaaaag tcaatgaaat cggagatttg aagggttttt tagttattaa tgagatttaa
      361 tttagtgatt cttatgaaaa tagttatact gggttttggt gcagtgggac agggagtaac
      421 ccgggtaata tcccttaaaa gggaatattt actggacaaa tacgggctcc agttggatat
      481 agtggcggcg gtggatcgat ccggagctgc tttatcagaa aatggactgg aaccagagac
      541 acttctagaa accaaggctg aaaccgggaa actatcacaa taccccgagt acggggtgga
      601 aggtgtttcc ggtttggaac tcttggatga agttgaatac gactgtctgg tggaagtgac
      661 acccaccaac attgaagatg gggaaccagc cctgagccat atgcagaagg ccatggaaaa
      721 gggtaaagac gtggttacct ccaacaaagg acccctggca ttatcattca ccaaactggc
      781 tgaaactgcc caaaagacag ggacgaactt caagtatgag gcctcggtgg gtggggccat
      841 gcccatcatc aacttggccc atgataccct ggctggtagt gatgttgaat cagtttacgg
      901 tatactaaac ggcaccacca attacatact ctcccgtatg gccaaggagg gctcgtccta
      961 tgaacaaacc ctgaacgaag cccgggaatt gggcattgca gaaactgatc ctagccagga
     1021 tgtggatggc tgggatgcag cttgtaaaat agtcatactg gcaaactcag ttttaaaccg
     1081 ggaatgtgca ctggaagatg tgaaggtgga gggcatatcc aagatcaccc cggaggccat
     1141 ggcattagct aaaaaagagg gatacttgat aaaattgata ggtgaagctt ctcaggatgt
     1201 tctggaggtt tctccccggc tggttaagga aggctccccc tttgcagtgg agggaactct
     1261 taacgtagcc accctgaaaa cggatttggc agaagatgta actgtggtgg gtaaaggggc
     1321 tggatccatt gaaactgcat ccgccatcct aagtgacctg ctgaacatct ggaattctgg
     1381 ttgactttac aggatctaat tattcataaa gatacgattt tcatgaagta cgtagtggtt
     1441 ataggggatg gtatggctga ttatcccctg gaggaactgg acggcaaaac acccctacag
     1501 aaggcaaaaa ctccgaatat ggatcgaatt gccagggaag gcgtttctgg tacgcttaaa
     1561 actgttccga atggtatgga agctggttca gatgtggcca acctctcaat tatgggctac
     1621 aacccccatg aatactacac tggacggggg cctctggaag cagccagtat gggagctaga
     1681 cttagaaggg atgaggtggc attcaggtgc aacctgataa acaccgaagc cgggctccta
     1741 tccgatttca acgccggaca catcagcacc cccgaggcat cggagattat agaatacctt
     1801 aatcagtcct tctaccagta tggtaagttc tacttgggca ctagctaccg gcacctcttt
     1861 gttttcccca gaaaggaggc tgcagatcta gaatccactc caccccatga tgtggtgggg
     1921 gagccacttt caaggcataa gttgaagcct gaagatgatg aactggcttg tgaattgaat
     1981 cagctaatgg accaatccca tggtaagctg gaaggccatc ccgtgaataa gaagagaagc
     2041 cagatgggta agaaaactgc caacatgatc tggttgtggg gccagggacg caaaccacaa
     2101 atgccaccat tcaaggctaa gtatggcctg aagggggcaa ccattaccgg cgtggacctc
     2161 atcaaaggca ttggagtcta cctgggactc accaacatta aagtgcctgg agcaacgggt
     2221 tactatgaca ccgattactg tgggaaggtg aactacgccc tggaggctct ggaagagcat
     2281 gacctggtgt tcatacacgt ggaggccccg gatgaggcag gacacgccgg tgatttagag
     2341 gaaaagatca gggccattga acggatcgac caccgcatac tgggcaaatt atggaaggaa
     2401 cttccccaac accatgattt tgccatggcc atcctccctg accacttcac ccccctatct
     2461 gtaaggaccc acaccccgga gccagtgccc tatgccatgt gcaaatccgg cggagtggtg
     2521 gatgaagtgg attcctacca cgaattttcg gtggcaaatg gttcacaggg cctccgggaa
     2581 ggccatctat ttttaaagaa cctcctggaa tactaatcac gtcaaaggta tatcaccaaa
     2641 aaaatcacga agaggtggga tgatccaagg taattccctg agatattaca ctgcagttct
     2701 ggccgggaaa acagcttcct tcattttaaa aaaggtcctg aagggtgaag ccagtgccat
     2761 gcctggtaaa gtggccatgc agatttaccc caacatcctc caagttgtgg atcagcgttg
     2821 ccagaagaag atactggtta ccggcaccaa tggtaaaacc accaccacca acctgttaca
     2881 ccacatcatc aaaagccagg gactccgggt gttatccaac ctacggggtg ctaacatgcc
     2941 ccagggactg gtaagctcct ttttaaatca tcaggatgcg gagtatgatt ggggagtttt
     3001 tgaggtggat gagggctcct tccaggaggt ggttaagcac cttaagccag actacgtggt
     3061 ggtgaccaac ttcttccggg atcagctgga ccgttatggg gagattgaaa aggcctttca
     3121 ggatatattt gacgccctta agcctttgga taccacatta atactcaacg ccgatgatcc
     3181 actggtttcc agctttaaaa gtctgaagaa gaggaacatc ttttacgggg tgggtgagaa
     3241 tcagttcagc accctggagg aaggagtaat agaaagccgt ttctgtccct cctgtgcctc
     3301 ccgcttggag tatgactatt tcaactacgg ccagttaggc ggatatcact gcagccagtg
     3361 tggcttcacc aatcccccac gggaatatga gataacccgg gtggaatact ctgaccccgg
     3421 ctaccacttg aagttcaaaa cagagaatgt taaatctatg gatcttgact tttcttacga
     3481 tggtatcttc aacacttaca actgctgcgc cgccctatct gtcgccctgg aaattggcat
     3541 ggaaactgga aaggttatag aaagtattgg cagatttgaa taccacttag ggcggatgga
     3601 aaccttccag ttcccggata aactggtaaa ggtggtgctg gtgaaaaacc ccattggact
     3661 gggggaggtg ttaaaaagcc tatccctgga tggtaaagtt aaatccctgc tcatggttct
     3721 gaatgataat cc
//
LOCUS       DAOQVW010000102         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27958_length_3730_cov_3.062585, whole genome shotgun sequence.
ACCESSION   DAOQVW010000102 DAOQVW010000000
VERSION     DAOQVW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27958_length_3730_cov_3.062585"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PKK85_03140"
     CDS             complement(<1..311)
                     /locus_tag="PKK85_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNS25047.1"
                     /translation="MNISDMLRKLVGHDCTGCGACTHNNIQIKWKEDKKKKDEDMKTS
                     PRLSQSVEDYLETMYCLDKDKGEIKVKDISQALNVKPPSVVEAVQKLSQMGLVSYQRY
                     R"
     gene            complement(313..2040)
                     /locus_tag="PKK85_03145"
     CDS             complement(313..2040)
                     /locus_tag="PKK85_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNS25048.1"
                     /translation="MKILLMGNPNVGKSVVFSRLTGVQVTSSNYPGTTVGYTKGTLKL
                     GEISAELIDVPGTYSLQPACQAEEVARKMLQEEDPDLVVSVLDATNLERNLFLTLQIL
                     EKGLPTLVVLNMWDCAQRRGVHLNIQGLSKALGVPVITLVAVTGEGLKELSEVMEEAL
                     KNPADYTPQISPMHDDERWAFIGQLITELQKIEHRHPTFLEKLEKASVHPVYGLIIAS
                     ITIILTLQLVIGLGEFLISNFLDPLYYQYYGPFITETVTNIFPSGIVHEILIGTGYQY
                     ETSFGILTTGVYVEFAVVLPYILSFYLFLGLLEDIGYLPRLAVLVDSLMHRLGLHGFS
                     IIPIILGFGCNVPAVLSTRMLEGKREKFIVAALISMSIPCMAQTAVIIGLLGVYGMQY
                     IAVVYGTLFILFISLGMLLNRILPGESPEIFVEIPPYRIPHLKALLQKTWSRVKGFLI
                     EAVPFVFLGILAINLLYILGIMDYLTILVSPLLSNLLGLPDDAIGALIMGFLRKDLAT
                     AMLAPLNLTAQQLTVATTFLAVSSPCVATFVVLLKELGVKDLIKVIMIMLGVALLTGT
                     ILNLLWSVV"
     gene            complement(2081..2308)
                     /locus_tag="PKK85_03150"
     CDS             complement(2081..2308)
                     /locus_tag="PKK85_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNS25049.1"
                     /translation="MTILNQLENGQKAIIIDINGGRGLIKRLESLNLRKGKEIRKISS
                     APFQGPIILEVDGCQLALGRGMAAKVTVKPL"
     gene            2486..2959
                     /locus_tag="PKK85_03155"
     CDS             2486..2959
                     /locus_tag="PKK85_03155"
                     /inference="COORDINATES: protein motif:HMM:NF036688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HNS25050.1"
                     /translation="MAKYSVETSIIISLVVGLIISNIPILGIRGMFSVIIVGFVASYY
                     TTPEKTSYKVGAMAGVTLAVVLLVISFLTPPDLPYTLPSATSLGVVVMANQFSILILG
                     IILSLLIYWFLGALGGYLATQFFNPPEAVDKTRYREPVRGVSKKRPRRTLERKKP"
     gene            2975..>3730
                     /locus_tag="PKK85_03160"
     CDS             2975..>3730
                     /locus_tag="PKK85_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS25051.1"
                     /translation="MTKVLIVGSGAGGGILASELSQRGVEVTIIEKGPSTPPGDAHCH
                     YDTINTGVEILKTHCVGGTTMVTAGNAVRTCQDELKKLNIDVGSELDQVEEEVGVTIL
                     PDSLLGEGTRILMDSARRLGLSMEKMPKFIDPRLCKPCGKCALGCPRNAKWNSRHYLK
                     NALNMGACLIENRPVVDVLLENGKLKGVRTHGKDYYADVVVLSSGAIATPRLLKRAGL
                     EAGAQLFVDTFITIGGVLEKIGFNEEVQMNALLR"
BASE COUNT          968 a          856 c          916 g          990 t
ORIGIN      
        1 tcccggtagc gctggtaaga gaccaggccc atctgggata atttctgcac tgcctccacc
       61 acacttggtg gtttaacatt caatgcctgg gagatgtcct ttaccttaat ttcaccttta
      121 tccttatcta gacaatacat ggtctctaga taatcctcca cactctgact taaccgggga
      181 ctggttttca tgtcttcatc cttcttcttt ttatcttctt tccattttat ctggatgttg
      241 ttatgggtac acgccccaca tccagtgcaa tcgtgaccca ctaacttgcg taacatatct
      301 gaaatattca atttacacca cactccacag gaggttcagt atcgttccag tcaggagggc
      361 tacccccaac atgatcatga tcacctttat cagatcctta acccctaact ctttgagaag
      421 aactacgaag gttgctacac agggagagct aactgccagg aaggtggtgg ccacggtgag
      481 ttgctgggca gtgaggttaa gtggtgctaa catggctgtg gctaaatctt tacgtaaaaa
      541 acccatgatg agtgctccaa tggcatcatc tggcaatccc aggaggttgg atagtagggg
      601 ggatactagg atggtgaggt aatccatgat acccaggatg tagagtaagt ttatggccag
      661 aattcccagg aatacaaagg gaacagcctc gattaggaat cccttaaccc ggctccaagt
      721 tttctgcagc agggctttga ggtggggtat ccggtaggga ggtatttcca caaatatctc
      781 cggactttct ccgggtagga tcctgtttag gagcatcccc agggatatga ataggatgaa
      841 gagagtccca taaaccacgg cgatgtactg cataccataa actcccagga gtccaatgat
      901 caccgcagtc tgggccatgc agggaatgga catggatatt aatgcagcga caatgaattt
      961 ttcacgctta ccctccaaca tcctggtgga gagtacggct ggtacgttgc aaccgaatcc
     1021 caggatgatt ggaattatgg aaaagccgtg caaccccaat cggtgcatta aactgtcaac
     1081 cagcactgct aaacgtggca ggtatcctat atcttccaat agtcccagga agagatagaa
     1141 ggatagtatg tagggtagca ccacagcaaa ttccacgtac acaccagtgg tgagtatgcc
     1201 gaaggaggtt tcgtactggt aacccgttcc aatgagtatt tcgtggacaa taccactggg
     1261 aaagatatta gtgactgttt ctgtaatgaa gggtccgtag tactggtagt agagggggtc
     1321 tagaaagttg ctaatcaaaa actcccccaa accaataacc aattgtaaag tgaggatgat
     1381 agtgattgat gctatgatta aaccgtaaac tggatgtaca gatgcttttt ctaatttttc
     1441 aaggaaggta gggtggcggt gttctatttt ctgtaattca gtgattagtt gccctataaa
     1501 tgcccagcgc tcatcatcat gcatgggact gatctgggga gtgtaatctg cggggttttt
     1561 caaagcttcc tccatgactt cagataattc cttcaaaccc tccccagtca ctgcaaccag
     1621 agttatgacc ggtactccca gtgccttgga gagtccctgg atgttgaggt gcacccccct
     1681 tctctgggca cagtcccaca tgttcagcac caccagggta ggcagtccct tttctagtat
     1741 ctgtaaagtt aaaaagaggt tacgctccag gttggtggca tccagtaccg aaaccaccag
     1801 gtccgggtct tcctcctgta acattttacg ggccacttct tcagcctgac atgcgggctg
     1861 cagggagtag gtgccaggga catctatgag ttctgcactt atctccccca gttttagggt
     1921 ccccttggta tagcccaccg tggtcccggg gtagttagag gaagttacct gtactccagt
     1981 gagtctggag aatactacgc tcttaccaac gttggggttg cccattaaga gaattttcat
     2041 gatgtacacc atttatgaat tacgtgcaaa gacagggtga ttatagtggt tttactgtta
     2101 ccttggctgc cattcccctt cccagggcaa gctggcatcc atccacctcc agtattatcg
     2161 gtccctggaa gggagctgag gatatctttc gtatctcttt acctttacga agatttagtg
     2221 attctaaacg ttttattaat ccacgtccgc cattaatatc gattatgatt gccttttggc
     2281 cattttcaag ttggttcaga atggtcatga tcatgcccca aataatatta ggtaagccta
     2341 actttttgtt aggataacct aactctttcc aggtatataa atcttcctat gaggatcatg
     2401 gatccaccta tttcccattc tttaagaaag cattataaga tatgccaggg aataatctct
     2461 agataaaaag aggtaaaaac attatatggc taagtacagt gtggaaacat cgattatcat
     2521 cagcctagtt gtgggtctca tcatctccaa cattcccatc ctgggtatcc gaggaatgtt
     2581 ctcggtaatc atagtgggat ttgtggccag ctactacacc acccctgaaa agacaagtta
     2641 caaggtgggt gccatggccg gagtaacctt ggccgttgta ctgttagtta ttagtttcct
     2701 gaccccacct gatctgcctt acaccctgcc cagtgccaca tccctgggag tggtagttat
     2761 ggccaaccaa ttttccatcc tgatactggg gatcatattg tcgcttttaa tttactggtt
     2821 tctgggggcc ctgggtggct acctagccac acagtttttc aacccccccg aggcagtgga
     2881 taaaacccgg tacagagaac cagtgagagg ggtgtccaag aaaaggccca gaagaaccct
     2941 ggagagaaaa aagccttaat tattggtgtt tactttgaca aaggtattga tagttggatc
     3001 tggggcggga ggtggtatcc tggccagtga actatcccag agaggggtgg aagttaccat
     3061 catcgagaag ggcccatcca caccccctgg tgatgcccac tgccattacg ataccataaa
     3121 caccggagtg gagatcctta aaacccattg tgtaggggga accaccatgg taacagctgg
     3181 taacgcagtg cgaacttgcc aggatgagct gaaaaaactc aacatcgatg ttggatctga
     3241 gcttgaccaa gtggaggagg aggtgggggt taccatcctg cctgactccc tgctgggaga
     3301 gggaacccgt atcttaatgg atagcgcccg gagactgggt ttatccatgg agaaaatgcc
     3361 caagttcatt gacccccgcc tgtgcaagcc ctgtggcaaa tgtgccctgg ggtgtcctcg
     3421 taatgccaag tggaattccc ggcactacct taaaaatgcc ctgaacatgg gggcttgttt
     3481 aatagagaac cgtcctgttg tggatgtgct cctggaaaat ggaaaactga agggagtgag
     3541 aactcatgga aaagattact atgccgatgt ggtggtgtta tcatcggggg ccattgccac
     3601 tcccaggctt ttgaagcggg cagggttgga agccggggca caactctttg tggatacttt
     3661 tatcaccata ggtggggtgc tggagaaaat tggattcaat gaggaggtgc agatgaatgc
     3721 acttctaagg
//
LOCUS       DAOQVW010000103         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_27960_length_3730_cov_2.783946, whole genome shotgun sequence.
ACCESSION   DAOQVW010000103 DAOQVW010000000
VERSION     DAOQVW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27960_length_3730_cov_2.783946"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..576
                     /locus_tag="PKK85_03165"
     CDS             4..576
                     /locus_tag="PKK85_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNS25052.1"
                     /translation="MIVVACVVLSVGIVMAAFSQYQELQTAQKSVKNYKERMSNPVNA
                     LDPISSVAGYLNYDLIIPKLGIMCNIRGDTVNAYNSVYHYPESVMPGKPGECGLLGHR
                     TTYSGLFRNISSLKVGDEVIIRDYVLHKKYVYQVSSNGADIRWNYKSNPIRFAQEGEP
                     RLLIITCYPPGKKQAAYIMHCQMVSSGSLK"
     gene            977..1228
                     /locus_tag="PKK85_03170"
     CDS             977..1228
                     /locus_tag="PKK85_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25053.1"
                     /translation="MVEIEIDEDACVGCGSCVDDCPNNVYQMNEETWKTTVVNADDCM
                     ACLSCHEICPSQTMTHRDIHVAKRLYIDRKVNDVLEKII"
     gene            1239..1793
                     /locus_tag="PKK85_03175"
     CDS             1239..1793
                     /locus_tag="PKK85_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrocarbon binding protein (contains V4R
                     domain)"
                     /protein_id="HNS25054.1"
                     /translation="MDMEMEEIRGTFKPEIIPRETGGKIGDYEAALHVLMKFVGSMSS
                     ALEQVSGRGANAIVYQAGKRMGHDAAKMLEKTDDLEKALNEMGEVLGVEFYFEMWKPK
                     GQEGYTIESGDKTEVKLLFMDCVVRQTLRRTGLPQKGPLCYLLYGYMVGAVEEVMSIK
                     GKLDIDHVGPNACLKTLTIKWGGK"
     gene            1793..2704
                     /locus_tag="PKK85_03180"
     CDS             1793..2704
                     /locus_tag="PKK85_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HNS25055.1"
                     /translation="MVTIALETLASCSGCEISILDLHEDLLKLLEKAELVYAPVLMDV
                     KELPDNIDIAIVSGSVRNEENKERLEELRTKSSILIAYGTCACYGGITGMADLYTRDE
                     VTSRTYSDNPSTESAPLPNEVVPELLSIVHPAADFTEIDGFIPGCPPKEQLTGDILIP
                     LINNEAPDVPKKSVCADCQREMEHVEYDKIHRRVEGSPEDGKCFLSQGYVCLGSVTLG
                     RCGGLCTDAGVPCHGCGGPSLDVLREPSHDIYNGVIKRIAHLSNMPEKDVEKQMYDLG
                     HVIYGFVIGSTIMEDKLVSKIPQLVKK"
     gene            2714..>3730
                     /locus_tag="PKK85_03185"
     CDS             2714..>3730
                     /locus_tag="PKK85_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNS25056.1"
                     /translation="MKNIEISPVTRIEGHAKITVQVDDGGNVVDAHFHVMEIRGFEKF
                     LEGAAVEEAPRITPRICGICQTSHHLASAKANDQIFGLEPPETAKKLRELMLLGQFIH
                     SHSLHFYFLGAPDLVMGPESDPAMRNVLGILKTNPELASMAIQTRKIGQEITAVVGGK
                     PISPVTAVPGGQSKGLTSEQQVDLLSKAKDAVELVEKGVEVTKPLFEQYGEAIELLGP
                     IETYFGALSRNGGDISLYDGDVKVIDAAGNLVHQFGSSDYLEYIEEKVQPWSYLKFPY
                     LKQIGFPQGNYRVGPLARLNVVDSVPTEKASELFAEYKEKYGIAQNPLLYHYARLIEL
                     MYASE"
BASE COUNT         1111 a          795 c          920 g          904 t
ORIGIN      
        1 tttttgatcg tagtagcctg tgtggttcta tcagtgggaa tagtaatggc agctttcagt
       61 cagtaccagg agcttcaaac agcccagaaa agtgtgaaaa actacaagga aaggatgagt
      121 aatccagtta atgccctgga tcccatctcc tcggtggctg gttatctgaa ctacgatctc
      181 ataattccca agctgggcat catgtgcaac atcaggggag acacggtgaa tgcgtacaac
      241 tctgtttacc attatcctga gagtgttatg cccggtaaac ctggagaatg tggattatta
      301 ggccatagaa ctacttactc tggactattc agaaatataa gttcactgaa agtgggtgat
      361 gaagttataa tccgggatta cgttctgcac aaaaaatatg tctaccaggt gtcctccaat
      421 ggggctgata ttcgctggaa ctacaaaagt aatcccatac gctttgccca ggaaggtgaa
      481 ccacggctgt taataataac ctgttatcct cccggtaaaa aacaggccgc ctacataatg
      541 cactgccaga tggtctccag tggcagcctg aaatagggtg tacatcctgt tccaaaagtg
      601 attacttagc accttttttt attagcaccc caaaagctat gttgttgata gaagacaata
      661 tatataataa aaaacattat tttgttgtga tgatagtcca ctttctagaa tctaacgaaa
      721 gagtccagag accacaccaa tgaaatcatg ttacacgctt aattccccag tgtgaaatac
      781 cgaatcatca tacaagtatg gtaaagggat ggaaacattt tttttaatac cagaaggtgg
      841 gtggggtatc aacatcatct tataagtact aggaatatca aaatattaag aaataatatc
      901 cactggcacg tagggagtga ttttggaata tctttcccgt aaacaaaaaa tgaaaaaaat
      961 atctagggga tttaaaatgg tggaaattga aatcgacgaa gatgcatgtg tagggtgtgg
     1021 ttcctgtgtt gacgactgtc ccaacaacgt gtaccaaatg aatgaggaaa cgtggaaaac
     1081 cacagtggtc aatgccgatg actgtatggc ttgcctatcc tgccatgaaa tctgtccatc
     1141 ccagaccatg acccaccgtg acatacatgt tgccaagagg ttgtacatcg accgcaaggt
     1201 aaacgatgtc ctggagaaaa taatctgagg ggatcagcat ggatatggaa atggaagaga
     1261 tacggggaac attcaaacca gagatcattc cccgagaaac cggaggcaaa attggagact
     1321 acgaagcagc actgcatgtg cttatgaagt tcgtgggttc catgtccagt gccctggaac
     1381 aggtgtctgg aaggggggcc aacgccattg tttaccaggc tgggaaacgg atgggtcatg
     1441 atgcggctaa aatgctggaa aaaactgatg acctggaaaa ggccctgaat gaaatgggcg
     1501 aagttttagg ggtggaattc tactttgaaa tgtggaaacc caaaggacag gaaggttaca
     1561 ccatcgaaag tggggataaa accgaggtaa aacttctgtt catggactgc gttgttcgac
     1621 aaacactgcg cagaacagga ttgccacaaa aaggaccact gtgctatctg ctctacggtt
     1681 acatggtggg agcggtggaa gaagtgatga gcatcaaggg caaactggac atagaccatg
     1741 tggggcccaa tgcctgtctt aaaaccctga ccatcaaatg gggtggtaaa taatggtaac
     1801 cattgcctta gaaacactgg ccagttgttc tggctgtgaa atatcaattt tggatctgca
     1861 tgaagactta ctcaaactcc tggagaaagc agaactggtt tacgcaccag ttttgatgga
     1921 tgtcaaggag ttaccagata acatcgatat tgccattgta tccggttcag ttagaaatga
     1981 ggaaaacaag gaaaggttgg aagagttaag gacaaaatct agcatcctaa ttgcctacgg
     2041 tacctgcgcc tgctatggag gtatcaccgg gatggctgac ctttacacca gggatgaggt
     2101 aacttcccgc acctactcag acaaccccag tactgaatcc gcccctctgc ccaatgaagt
     2161 ggtgccggaa ctcttaagca ttgtccaccc tgctgcggac ttcactgaaa tcgatggttt
     2221 cataccaggc tgccccccta aggaacagtt aactggagat attctgatac cactaataaa
     2281 caatgaagca ccagatgttc ctaaaaagag tgtttgcgct gattgccagc gggaaatgga
     2341 acacgtggaa tacgataaga tacaccgccg ggtggaagga tcacccgagg atggtaaatg
     2401 tttcctgtca caggggtacg tctgtttggg ttcagtcacc ctgggacgct gcggcggatt
     2461 gtgtacggac gctggtgttc cctgccatgg ttgtgggggt ccatccctag acgtgctgag
     2521 agagcccagt catgacatct acaacggagt tattaagagg attgctcacc tatccaacat
     2581 gcccgaaaaa gatgttgaaa aacagatgta cgatctgggg cacgtgatat acggatttgt
     2641 tataggaagt accatcatgg aggacaagct ggtttcaaag ataccccagc ttgtgaaaaa
     2701 gtaggtgatg atcatgaaga atatcgaaat aagtccggtt acacgtattg aaggacatgc
     2761 caaaatcacc gtgcaagtgg atgatggagg gaacgtggtt gatgcccact tccacgtgat
     2821 ggagatccgt ggatttgaaa aattcctgga gggcgctgcc gttgaagaag caccacgtat
     2881 cactccccgt atctgcggta tatgccaaac ctcccaccat cttgcatcgg ccaaggctaa
     2941 tgaccagata tttggtctgg agccaccgga aactgctaaa aagttaaggg aactcatgct
     3001 actgggacaa ttcattcact cccattctct tcacttctac tttttaggcg ccccggatct
     3061 ggtgatggga cctgaatctg accccgccat gcggaacgtc ctggggatcc ttaagaccaa
     3121 ccccgaattg gcttctatgg ccatccagac cagaaaaatt gggcaggaaa tcacagcagt
     3181 ggtgggtggg aaacccatca gtcctgtaac tgccgtgcct ggaggacaat ccaagggatt
     3241 gacctcagag caacaggttg atttattgtc gaaagccaaa gatgcagtgg aacttgtgga
     3301 aaaaggggtt gaagtaacta aaccattatt tgaacagtat ggtgaagcaa ttgaactact
     3361 tggaccaata gaaacatatt ttggagcctt gagcaggaat ggcggcgata tcagcctata
     3421 tgatggggat gttaaggtta ttgacgctgc tggtaacctt gtgcaccagt ttggttcctc
     3481 ggactatctg gaatacatcg aagagaaagt ccagccctgg tcttacctga aatttcccta
     3541 cctgaagcag atagggttcc cccagggcaa ctaccgggta ggacccttag cccggttaaa
     3601 tgtggttgat tctgttccca ccgagaaagc ctcagaactc tttgctgaat acaaagaaaa
     3661 atatggaata gcccaaaacc cattgcttta ccactacgct cgcctcatag agctcatgta
     3721 cgcttctgaa
//
LOCUS       DAOQVW010000104         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_28073_length_3719_cov_3.176037, whole genome shotgun sequence.
ACCESSION   DAOQVW010000104 DAOQVW010000000
VERSION     DAOQVW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28073_length_3719_cov_3.176037"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PKK85_03190"
     CDS             complement(<1..165)
                     /locus_tag="PKK85_03190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262524.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. The beta
                     subunit is part of the catalytic core which binds with a
                     sigma factor to produce the holoenzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HNS25057.1"
                     /translation="MVKNAWELVDAFFNDYKLVDHHLKSYNDFVDHRIQDIIDITEPI
                     VLEQGDYSIQT"
     gene            complement(284..520)
                     /locus_tag="PKK85_03195"
     CDS             complement(284..520)
                     /locus_tag="PKK85_03195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HNS25058.1"
                     /translation="MVKKDILKHELVPDHVVLKKNQITKVLKDMGVHPEQLPKIKLDD
                     PVVKAIGAKKGDIIKITRKSQTAGRFISYRLVLG"
     gene            complement(546..619)
                     /locus_tag="PKK85_03200"
     tRNA            complement(546..619)
                     /locus_tag="PKK85_03200"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Lys,seq:ttt)
     gene            complement(626..697)
                     /locus_tag="PKK85_03205"
     tRNA            complement(626..697)
                     /locus_tag="PKK85_03205"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(662..664),aa:Asp,seq:gtc)
     gene            complement(869..941)
                     /locus_tag="PKK85_03210"
     tRNA            complement(869..941)
                     /locus_tag="PKK85_03210"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(906..908),aa:Tyr,seq:gta)
     gene            complement(949..1023)
                     /locus_tag="PKK85_03215"
     tRNA            complement(949..1023)
                     /locus_tag="PKK85_03215"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(985..987),aa:Pro,seq:tgg)
     gene            complement(1073..1146)
                     /locus_tag="PKK85_03220"
     tRNA            complement(1073..1146)
                     /locus_tag="PKK85_03220"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1110..1112),aa:Thr,seq:tgt)
     gene            complement(1249..2484)
                     /gene="pgk"
                     /locus_tag="PKK85_03225"
     CDS             complement(1249..2484)
                     /gene="pgk"
                     /locus_tag="PKK85_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNS25059.1"
                     /translation="MVNHSLDFYTLDDLKIEGKTILVRVDINSPVDPNTGLILDDTRI
                     RLHAETISELSSRDAKTVILAHQSRPGKNDFTTLSQHAEILSAILGKTVRYVDDIFGS
                     NARQSISRMKPGDIILLENVRFYSEEVLKRDPPQQAETHLVRKLAPLVDFFINDAFAA
                     AHRSQPSLVGFSFDMPSVAGRVMEKELRALYSAVKSVKRPCVFILGGVKVDDSIKVME
                     NALETGNADYVLTTGLVANIFLWGAEINIKKYNRNFIKDRDYCDYVKMAKKLIKKFPE
                     QIKIPLDLAVYAEGQREEHPVKKLPNLPIYDIGTDTIAEYVQIIRQARTIFANGPAGV
                     FEKEGFNIGTEDVLNAISSSTGFSIIGGGHLAAAANRMGLKGISHISSGGGASISLVA
                     GEKLPAVEILRESALRYRL"
     gene            complement(2517..3188)
                     /gene="tpiA"
                     /locus_tag="PKK85_03230"
     CDS             complement(2517..3188)
                     /gene="tpiA"
                     /locus_tag="PKK85_03230"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNS25060.1"
                     /translation="MNGKPLVILNFKTYLESTGLNALKIAKASEEVADETGVVIAVAT
                     QAPDLWRISREVNIPVFAQHVDAIDSGGHTGSILLESVKQAGAQGTLINHSEQRMELA
                     DMEMVVKKANQADMVSVACTNNVDTSAAAAILKPDFVAIEPPELIGSGIPVSQAEPEI
                     VEGTVEVIHDISPEVGVLCGAGISTGDDMKAALDLGTEGVLLASGIILADDPKEALLG
                     LVSKL"
     gene            complement(3436..>3719)
                     /locus_tag="PKK85_03235"
     CDS             complement(3436..>3719)
                     /locus_tag="PKK85_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudomurein-binding protein"
                     /protein_id="HNS25061.1"
                     /translation="ARYRYSGAAHDGTAMEKNGAGDCWAMSDYLQTKFTKAGIKSRIV
                     QYSNAYSSRHRSVQLFQNNKWLDVPYKQYGINQMFSAQSSKPGIKVITG"
BASE COUNT         1006 a          873 c          731 g         1109 t
ORIGIN      
        1 tgtttgaata gagtaatcac cctgttcaag gactataggt tctgttatgt caattatatc
       61 ttgtatacgg tgatcaacaa agtcattata agactttagg tgatgatcaa ccagtttgta
      121 gtcgttaaaa aaggcatcta ccaattccca ggcattttta accattaaat tcctccaaaa
      181 taaatacacg tcaataaaat attttaatcc tataaatttt ctccaattta ttaatattat
      241 atatacaatt ggctatttat aattggcttt tactaatact atatcatccc agtaccaatc
      301 gataacttat gaatcgccct gcagtttggc tttttctagt aatttttatg atatccccct
      361 ttttggcacc aattgccttt accacaggat catccaattt tatttttgga agctgctcag
      421 ggtggactcc catatctttg agcaccttag ttatctggtt tttcttcaaa acaacatgat
      481 ctggaacaag ttcgtgtttt aagatgtcct tcttcaccat ttagttcctc caagaaaaat
      541 tagaacgggc ccgacgggaa ttgaacccgc gaccacttgg ttaaaagcca agcgctctcc
      601 cagactgagc tacgggccct ctagacgccc tggccgggat ttgaacccga gtcgtgggct
      661 cgacaggccc acatgatagc ccctacacca ccagggcaac tcatagaatg agagtattga
      721 gagtttaact atgcttactt atatactttt ctttgaatcg tacagatatc tagcctacca
      781 tcggttattt atacataaat tgtgcaggtt agaggatgcc cacagatcgt atccataaat
      841 tcttaagttc tacacatcct attactagtc ccgcctgccg gacttgaacc agcaaccctc
      901 ggatctacag tccgatgctc tgccaattga gctaaggcgg gttttggatg ggaccacccg
      961 gatttgaacc ggagtctcag gctcccaaag cccaaaggat cgaccaagct accctatggt
     1021 ccccttaata gatgtctgtt ttcaagtgag tcatgattca tactacgcct agagccccgg
     1081 acgggaattg aacccgcgac cacgagatta caagtctcgc gctccaccag actgagctac
     1141 cgaggcatag ttagagcaac taaatttaag aaaatattcc atataaagtt ttgggtagca
     1201 cagactagat tttacacccc acagttctcc tggtcataga aagtttcact acaaccggta
     1261 tctcagggcc gattctcgta aaatctcaac tgccggtagc ttttcaccag ccaccaaact
     1321 tattgatgcc cccccaccac tactaatatg gctgataccc ttaagtccca tgcgattggc
     1381 tgccgcagcc agatgaccac cccctatgat ggaaaatcca gtggaagatg agatggcatt
     1441 caggacatct tctgtgccta tgttgaaacc ttctttttca aagacccccg ccggaccatt
     1501 ggcaaaaatt gtacgggctt gcctgatgat ctgtacatat tctgcaatgg tatcagttcc
     1561 aatatcatat atgggaaggt taggaagttt cttaaccgga tgttcctccc gttgaccctc
     1621 tgcatagacc gccagatcca gaggtatctt aatctgttct ggaaacttct taatcagctt
     1681 tttagccatt ttaacgtagt cacaataatc acggtctttg atgaagttcc ggttatactt
     1741 ttttatattt atttctgcac cccacaggaa gatgttggcc accaaccccg tggtcaggac
     1801 atagtcagca ttacctgttt ccagtgcatt ttccatgacc ttgatggaat catcaacttt
     1861 aactccccct agtatgaaaa cacagggtcg ctttacactt ttaactgcac tgtaaagtgc
     1921 tctaagttcc ttttccatta ctcttccggc cacggaaggc atgtcgaagg aaaatcccac
     1981 cagggatggt tgagaacggt gggcagcggc gaaagcatcg ttaataaaaa aatcaacaag
     2041 tggggccaat tttcttacca gatgggtttc cgcttgctga ggtggatcac gtttcaagac
     2101 ctcctcggag tagaagcgaa cattctccaa gagaatgata tcacctggtt tcatccgaga
     2161 tatggattgc cgggcattac taccaaatat atcatctacg tatctcaccg ttttacccaa
     2221 gatggcagat aatatctccg catgctggct cagggtggtg aaatcattct tacctggacg
     2281 gctctgatgg gccagtataa ctgttttagc atccctggag gaaagttcgc ttatggtttc
     2341 ggcgtgcaga cgaattcgag tatcatccag aatgaggccg gtattaggat ccaccggtga
     2401 atttatgtca accctcacca ggatggtttt accttctatt ttcaagtcat ccagggtata
     2461 aaaatccagg gaatggttaa ccattattta cacctttatt taaatttgtc aagcgcctat
     2521 aacttgctca ctaaacctag aagggcttct ttaggatcat cagccagtat gatacccgat
     2581 gccagaagca ctccttctgt tcccagatcc aaggcggcct tcatatcatc accagtagat
     2641 atccccgcac cacagagaac tcccacttca ggactaatat catgaataac ctccacagtt
     2701 ccctccacaa tttctggttc tgcctgggat actgggattc ccgagccaat tagctctgga
     2761 ggttcaatgg ctacgaaatc aggttttaga atagctgcag cagcactggt atccacgttg
     2821 ttggtgcaag caacactcac catgtcggct tggttagctt ttttgaccac catctccata
     2881 tccgcaagtt ccattctttg ttcagagtga ttaattaagg taccctgagc acctgcttgt
     2941 tttaccgatt ccaagagtat gctgccagtg tgtccaccag aatctatggc atcaacatgc
     3001 tgagcaaaga ctgggatgtt aacttcccgg gatattctcc acaagtccgg ggcttgagtt
     3061 gccactgcta tgacaacccc tgtctcatca gccacttctt ctgaagcttt ggcaattttt
     3121 agggcattta atccagtaga ttcgagatat gtcttaaaat ttagaattac cagaggtttt
     3181 ccattcattt tcataatacg cccccaataa aggtctagta ttgtttattt ctttataacc
     3241 ggatcataaa aggattgtgg tttttccctt atgataaata gagcatttat tcatcctata
     3301 tccagcaaaa aaattacttt aatgcgatat tgatacaaga agcgatgatg atgcttaaaa
     3361 caaaaaaaga gtgtatcaac agttgtgcat ttaactgttg attattggtt ttatatctca
     3421 gtaaaagtgt ttttatcaac cagttatgac tttaatgcct ggtttacttg attgggcact
     3481 gaacatctgg tttatgccgt attgcttgta aggtacgtct agccatttgt tattctggaa
     3541 gagttgcacc gaacgatgcc gagatgagta agcattggag tattgaacta tcctggactt
     3601 gatgcctgct ttggtgaatt tggtttgtaa atagtcgctc atagcccagc aatcgcctgc
     3661 tccatttttt tccatggctg taccatcatg ggcggcaccg ctgtatcggt accgtgctg
//
LOCUS       DAOQVW010000105         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_28343_length_3694_cov_3.351195, whole genome shotgun sequence.
ACCESSION   DAOQVW010000105 DAOQVW010000000
VERSION     DAOQVW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28343_length_3694_cov_3.351195"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PKK85_03240"
     CDS             <1..951
                     /locus_tag="PKK85_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNS25062.1"
                     /translation="QADYTITFHRKKTGIEKASSKYVGYVQVCDIGIPLDAEVCTGPG
                     DLIRLQERDINSHKGQNGRVLVIGGGQDYSGAPALAALAALGSGVDISVVVCPKIVAS
                     EIRSFSPDLIVKSLPSHAILPSDVDKILKLAANMDSVIMGCGIGTEEGTRVAVNQIVK
                     NIRKPILLDADALKLVDLDQVGKGLELVLTPHSSEFKTVFAKSVPENLEDKIDLIKNI
                     TLKKSFTVVLKGNTDVISFNGLFKLNKTGNPGMTVGGTGDCLAGLVGGLMAQGHPGFE
                     SAFLGAYINGRAGDQAIIEYGNNFTATQLLDFIPQAMRLD"
     gene            complement(1003..1263)
                     /locus_tag="PKK85_03245"
     CDS             complement(1003..1263)
                     /locus_tag="PKK85_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HNS25063.1"
                     /translation="MGLLEIVFIGLILVILGIVGVRILWRVGQVVLSVVFHMLLGWVL
                     LFIWNILPFFKIPITILTVLVAGLGGVLGVGILVLAKALGLY"
     gene            complement(1276..2454)
                     /locus_tag="PKK85_03250"
     CDS             complement(1276..2454)
                     /locus_tag="PKK85_03250"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNS25064.1"
                     /translation="MEKVVLAFSGGLDTSVCVKLLQDKYQKEVITACVDVGQPVDEIS
                     RPEKLARDMGVKHYTIDAKNEFAREFIFRAVKANALYEGYPLSTALARPLIAMKIVDL
                     AKKEGATAISHGCTGKGNDQFRFESTIRSGSNCEVIAPIRDLNLTRTEEMEYAREHGI
                     PLPSEKMYSIDENLWGRSIEGDILEDPMVETPEEAFSWTRSTEDAPEDAQILEMSFEK
                     GVPVSLDSQDMGPVEIIEACNQVAGSHGIGRVDIIEDRIIGLKSRENYETPGALLIIN
                     AHKALEQLTLTREELKFSEIVSLTYSELVYNGLWHEPLREDLDQLLDHMQQRVSGVVK
                     LKLHKGSLRILGRKSPYSLYNEKAVSFEDKELDQREIAGMVKNYALQAACYQDICKRD
                     "
     gene            2537..3289
                     /locus_tag="PKK85_03255"
     CDS             2537..3289
                     /locus_tag="PKK85_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNS25065.1"
                     /translation="MNLEMTIISRDTGGTIEDNHTLMQYLINNPLTKAIVDTAKKGTP
                     LLKLGKGTPRAMLISGIHGNELPPQISSIRLINEINLIKSRGTIYFIPFAIPKATMQN
                     YRRFDGFDMNRSAFKSGTISNDILELLESLKIDALADFHSTQKNSNPGVQSIFCSKEP
                     CYESFKMAKYMAQITSSKIICQEKAGTLFTGALEDEANLKGIPAVTCEVVSKNGWVDK
                     GSPEHSYHQMRTFLDYVGVELVGLQSVFPPDP"
BASE COUNT         1043 a          838 c          751 g         1062 t
ORIGIN      
        1 caagctgatt ataccatcac cttccaccgg aagaaaactg gaattgaaaa ggccagctct
       61 aaatatgtgg gttatgtaca ggtgtgcgac ataggaatcc cccttgatgc agaggtttgt
      121 accggtcctg gagatttgat tcggcttcag gaaagggata taaattcgca taagggtcaa
      181 aatggtcgag ttctagttat aggtggtggt caggattact ctggtgcccc ggctctggct
      241 gctctggctg ctctgggaag tggtgtggat atatccgtgg tggtctgtcc aaaaattgta
      301 gcttctgaga tacgttcttt ttctccagat ctcattgtta aaagtcttcc ttcccatgca
      361 attctccctt ctgatgttga taagatactg aaattggcgg ctaacatgga ttcggtgatc
      421 atgggttgtg ggattggcac cgaagaagga actagagtgg cagtcaacca gattgtgaaa
      481 aatataagaa aaccaatttt gttagatgcc gatgctttga agttggtaga tctggaccag
      541 gttggtaagg ggttggaact ggtcctaaca ccccatagtt cagaattcaa gacggtgttt
      601 gctaaatcgg tacctgaaaa tctggaagat aaaatagatt tgatcaaaaa tataactctt
      661 aaaaaatcat tcacagtcgt tttaaagggt aacaccgatg tgattagttt taatgggcta
      721 tttaaactca ataaaactgg aaatccaggt atgactgtgg gtggaactgg agactgtttg
      781 gctggcttgg ttggtggcct gatggcccag ggtcatcctg gtttcgaatc tgcattcctg
      841 ggtgcctata tcaatggtcg ggctggagat caagctataa tagagtatgg taataacttc
      901 actgccactc aactcttaga tttcattcca caggcaatga gactggatta aaaaacagaa
      961 cttgaaaata actttcataa acgggtttaa acactttaca tcctagtaca aacctagtgc
     1021 tttggccaac actaggattc ccactcccag tacaccaccc aaaccagcaa ccaggacagt
     1081 taaaatggtt atgggaattt tgaagaaggg tagaatattc cagatgaaaa gcagcaccca
     1141 acctagaagc atgtgaaata cgacactgag caccacttgg cctaccctcc aaagaatcct
     1201 taccccaaca atacctagaa taaccagtat taacccaatg aacacgattt ccagaagacc
     1261 catgtgttaa cctatctaat cccttttaca gatatcctga taacaagcgg cctgaagagc
     1321 atagttttta accatgcctg ctatttccct ctgatccaac tctttatcct cgaaagagac
     1381 tgctttttca ttgtaaaggc tgtatgggga ttttcttccc aggatgcgca gacttccctt
     1441 atgcagtttg agcttaacca cccctgaaac cctttgctgc atatgatcca gtaactgatc
     1501 cagatcttcc cgcagtggct cgtgccacaa accattgtag actaactcag aataggttaa
     1561 agaaactatt tcactgaatt taagttcctc ccgggtaagg gttaactgtt ctaatgcttt
     1621 atgggcattt attattaaca aagcgccggg ggtttcgtag ttctccctag atttcaaacc
     1681 tataatacga tcttcaatga tatccacccg gccaatacca tgtgaaccag ccacctgatt
     1741 acaggcctca ataatttcta ccggccccat atcttgactg tcaaggctca caggaactcc
     1801 tttttcaaag gacatctcca ggatctgagc atcttctgga gcatcctctg tggagcgggt
     1861 ccaactaaag gcttcctcag gagtttccac catgggatcc tccagaatat ctccttcaat
     1921 ggaacgaccc cacaaattct catcgatgct gtacatcttt tcagagggga gtggtattcc
     1981 atgttccctg gcatactcca tctcttcagt ccgggttaaa ttcagatcac gaatgggagc
     2041 tatgacctca cagttggatc ctgatcgtat ggtggactcg aaacggaatt gatcattacc
     2101 cttaccagta catccatggg aaatggcagt tgcgccttcc tttttggcca ggtccacgat
     2161 tttcatggcg atgaggggtc gtgccagggc agtgctaagg ggataaccct cgtaaagggc
     2221 gttggccttc acagcccgga atatgaattc cctggcaaat tcgttttttg catcaatggt
     2281 gtaatgtttc acccccatgt cacgggccaa tttctccggt cgtgatattt catccacagg
     2341 ctgaccaaca tcaacacaag cagttataac ctctttctga tatttgtctt gtaataattt
     2401 gacacataca gaggtgtcca ggccaccact gaatgcaagg accactttct ccattttcca
     2461 atcaccttga ttatttatat gaaaattaaa atcatatctt atatttacag aggaccaatt
     2521 taaaacttgg tgtagcatga atctggaaat gaccatcata tcccgggata caggaggtac
     2581 catagaggat aaccatacct taatgcaata tttaattaac aatcctctga ccaaagctat
     2641 tgtagacact gctaaaaagg gcacgcctct tttaaaatta ggaaaaggta ctcctagggc
     2701 gatgcttatc tctggaatcc atggcaacga attacccccc caaatatctt caataagatt
     2761 aatcaacgag attaatctaa ttaaatcacg gggaacgatt tacttcatcc cctttgccat
     2821 accaaaggct actatgcaaa attaccgccg ttttgatggt tttgatatga accgcagtgc
     2881 cttcaaatca ggcaccatct ccaatgatat tcttgaacta cttgaatccc taaaaataga
     2941 tgctttagcc gactttcatt ccacccagaa aaacagcaac cctggggttc aaagtatttt
     3001 ctgttccaag gaaccctgtt acgagagttt taaaatggca aaatacatgg cacagatcac
     3061 ctcatccaag ataatatgcc aggaaaaggc gggaaccctc ttcaccggcg ccctggaaga
     3121 tgaagcaaac ttgaagggaa ttccggcagt tacctgtgaa gttgtatcca agaatggatg
     3181 ggttgataag ggaagtccgg aacattccta ccatcaaatg agaacattcc tagattatgt
     3241 gggtgtggaa ttggtgggac tacaaagcgt cttcccacct gatccctgat atgatttaat
     3301 ggccgttaaa aagatctgta tttaagtaaa atgccatctt ataagaacca tttactcttc
     3361 tcactactaa ttaccatggt agtgactgcc cccttttttc ccaatgtttt ttacctttcc
     3421 ctggctgtga taggtgcttc cattgtggat ctggatcacc cattgagaaa tagaaacctg
     3481 ctactcctag gcctttttgg attaatttta gtactggtac tgcatattct ccacagatcc
     3541 atcctactgg gcttaatcct tatcttgcta tcgttgacat tttttgtatc aacccatcgc
     3601 ggtttcatgc attccatttg gggtgtgacc ttcattgctg ctctgttaac tatgtttacc
     3661 tacggcgcat tccaatttct aattaccttt tctg
//
LOCUS       DAOQVW010000106         3679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_28511_length_3679_cov_3.431843, whole genome shotgun sequence.
ACCESSION   DAOQVW010000106 DAOQVW010000000
VERSION     DAOQVW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28511_length_3679_cov_3.431843"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1047)
                     /locus_tag="PKK85_03260"
     CDS             complement(184..1047)
                     /locus_tag="PKK85_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNS25066.1"
                     /translation="MDNRSGMGWLKDYPDFRDYSMGKKSLKPLIERMGIVDPLKTAPK
                     SIDLREWCSPVEDQGQLGSCTANAGVGMVEYFEKRAFAKHLDASRLFLYKATRNLMKV
                     TGDTGAYLRTTMQALVLFGVPPEEYYPYRVEDFDLEPPAFTYSFAQNYQALKYLRLDP
                     LQADRKILLERIRTNLTAGLPSIFGFTVYNSIHDSGDTGMIPYPCKKEQVLGGHAVMA
                     VGYDDNMVITNSSCKNKTTGAILIRNSWGTAWGEEGYGWLPYEYVTKGLATDWWTLIK
                     NEWVDTEQFGL"
     gene            1400..2995
                     /locus_tag="PKK85_03265"
     CDS             1400..2995
                     /locus_tag="PKK85_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25067.1"
                     /translation="MELINYRKYSLAVPALVALLLALIPTLNYQWPLSWDIIYHVQYA
                     KVYSQYGFTLTTPLLNAPVGQKIGYPPLFHFLLVGLGGLFQANYFQVARWLQPVLATL
                     LVFSTSYVAFKFYGRLAGVSTGFILVSSYLFKRMILPVPENLALIFIPLAVYLYYLSI
                     RDGKLKIAALSGLILILILGIHLAAALTLLLTVTGISIVEFVYYRDLTVFRNYGAFIL
                     ALIVFISTALLGMFFLYPGVFESILAQGITSATGFATSLVSSRPLSIPGYLGNLGPFA
                     SVFGFLGLIWALQRRRKKDLFVITWIVGMLLLSVSYIIGVNVISYRVMIYLLIPLSIL
                     AGVGLQQFYHYLKNYRRLSSEKFRCTFLVLLLLLATLSGFLTVINPDIAIFSVKSDYG
                     DVQIAPPSDAELDLNQWIQENGNHSRSILISNQFTGMLLATESGMPMHYGFEYYGLKS
                     LPTATLDAMRNESIGYIIYDKKLVLSPPDENGLYIKSVYYEFFPLFYFSQDIEKNIDR
                     IKPEYTQVVYENQEFIVCQVLYT"
     gene            complement(3004..3255)
                     /locus_tag="PKK85_03270"
     CDS             complement(3004..3255)
                     /locus_tag="PKK85_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25068.1"
                     /translation="MSIKENYGRYWLAGFIFSIILGAITTVLNFFSTNISSYAMNSLP
                     GLIGGFSLLITGVLIIIILPWIVGRLVAWIYQEFIDKKP"
     gene            3424..>3679
                     /locus_tag="PKK85_03275"
     CDS             3424..>3679
                     /locus_tag="PKK85_03275"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032996.1"
                     /note="catalyzes the formation of oxaloacetate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HNS25069.1"
                     /translation="MTKIRIIETAYRDAHQSLLATRMRTRDMLPIAEEMDKVGYFSLE
                     SWGGATFDSCIRYLNEDPWERLRELKEHVTKTPLQMLLRGQ"
BASE COUNT         1005 a          830 c          695 g         1149 t
ORIGIN      
        1 tacttcaata tcggctttta ccatgaaaca cccaccgaaa cagtacaccg atgatctggc
       61 ccgggaaatg gtggatgagt tcattgtcgg taccagggaa agatagtttt ttaattttta
      121 gatagaaaaa attcgaataa agtgaaatac tcatctattc actttattct tgaaaaatta
      181 ctcttataaa ccgaattgtt ctgtgtccac ccactcattc tttatcaatg tccaccaatc
      241 agtggccaat cctttggtaa catattcgta gggtaaccaa ccatatccct cttctcccca
      301 ggctgtaccc catgagttac gtattaatat agctccagtc gttttatttt tgcaggaaga
      361 gtttgttatt accatgttat catcgtagcc cacagccatc acggcatgtc ctcccaggac
      421 ttgctctttt ttacaggggt aggggatcat accagtgtcc ccagaatcat ggatagaatt
      481 gtaaactgtg aaaccaaata tagatggcag tccagcggtt aaattggttc tgatcctttc
      541 tagaaggatc tttctatcag cttgcagagg atcaagacgt aggtacttta aagcctgata
      601 attctgggca aatgagtagg tgaaggctgg tggttcaagg tcaaagtcct ccacccggta
      661 gggatagtac tcctcgggag gcactccaaa aagtaccaga gcttgcatgg tagtccgtaa
      721 ataggccccc gtgtccccgg taaccttcat gagatttctg gtggccttgt ataaaaacag
      781 ccgcgaggca tccagatgct tagcaaatgc tcttttttca aaatattcca ccataccaac
      841 tccggcattg gcagtgcagg agccgagctg cccctgatct tccactggag aacaccactc
      901 cctgagatct atacttttag gagctgtttt taacggatca actattccca tcctttcaat
      961 cagcggtttt aaactctttt ttcccatact gtagtccctg aagtcagggt aatcttttaa
     1021 ccatcccata ccacttctat tatccacatt catcacctcc aagtattgat gatcttcacc
     1081 catcatcacc tagtaataat ttatcatgaa aaaccaagtt ggtgaattat aattactgct
     1141 tcataaggca atatcgcgcc gttgggtttt taaaaaactt tgttctacta tttaatatta
     1201 taaaactaat ctttattaat ttttttggct tcttaattga aaatcttgat tttatccaat
     1261 aataaagtca taaatttata taataaaaac tgggttgatt ctttgattcg aagtggaaat
     1321 ctttttatcc acttccaccc ccaatatcaa cactgggttt ctagattttt ttatcttcat
     1381 taaattccag gaggaaatga tggaacttat aaattacaga aaatactccc tggctgtccc
     1441 ggcactggtt gctctgctac tggccctgat ccccaccctg aattatcagt ggcccctcag
     1501 ttgggacatc atctaccacg ttcaatacgc caaggtctac tcccagtacg gtttcacctt
     1561 gaccaccccc ctcttaaacg cccctgtagg tcaaaaaata ggttatcccc ctctattcca
     1621 cttcttgttg gtgggtctgg gtggtctgtt tcaggccaac tacttccaag tcgcccgttg
     1681 gttacaacca gttttagcta ctttgttagt attttccact tcctatgtgg ctttcaaatt
     1741 ctatggaaga ttggctgggg tgtcgacagg gttcatcctc gtatcaagct atctgttcaa
     1801 gaggatgatc ctaccggttc cagaaaactt ggctctgatc ttcatccccc tggcagttta
     1861 tctttactac ctttccataa gagatggaaa acttaaaata gcagctttat caggtttaat
     1921 acttatattg atactaggaa tccatttagc agctgcatta accctgttat taactgtaac
     1981 tggaatcagc attgttgaat tcgtttatta ccgggatctc actgtattta gaaactatgg
     2041 agctttcatc ttagctttaa tagtatttat tagcactgca ctcctgggta tgtttttcct
     2101 gtacccagga gtatttgaat cgattttagc ccagggaata acatcagcca caggctttgc
     2161 aacttccctg gtatcaagcc gtccccttag catcccagga tatcttggga atttaggtcc
     2221 ctttgcaagt gtttttggat ttttagggtt aatatgggca ttgcagcggc gcagaaaaaa
     2281 agatctattt gtaattacct ggatcgtggg catgcttctt ctaagcgttt catatattat
     2341 tggtgtcaac gtgatatcct accgggtaat gatctacctt ctcattcccc tatccatact
     2401 ggctggagtg ggcctccaac aattttatca ttaccttaaa aattatcgtc gtttatcatc
     2461 ggaaaaattc agatgcacct tcctggtgtt gttgttacta cttgcaactt taagtggctt
     2521 tttaacggtg atcaatccgg acattgccat tttttcagtt aagagtgact atggtgatgt
     2581 gcaaattgca ccaccctctg atgctgagtt ggacctcaac caatggatcc aggagaatgg
     2641 taaccacagc cgttccatcc ttatatccaa tcagttcaca gggatgttac tggccaccga
     2701 gtccgggatg cccatgcact acggttttga gtattatggc cttaaaagtc tacctaccgc
     2761 tactttggat gccatgagga atgaaagcat tggttacatc atttacgaca aaaaattggt
     2821 cctttcaccg ccagatgaaa acggactcta tatcaaaagt gtttattatg aatttttccc
     2881 ccttttctac ttcagtcagg atattgaaaa aaacatagac cgtatcaagc cagagtatac
     2941 ccaggtagtc tacgaaaacc aggaattcat cgtttgccag gtactttata cttaatattt
     3001 tttctaaggc tttttatcaa tgaactcttg gtaaatccaa gccaccagtc gtccaactat
     3061 ccacggcagg attataatga ttaaaacccc tgttatcagt agtgaaaagc cgccaattaa
     3121 tcctggcagt gaattcatgg cgtaactact tatatttgta ctgaaaaagt ttaaaactgt
     3181 ggttattgct cccaggatga tgctgaagat gaatcccgcc agccaatacc ttccgtaatt
     3241 ttctttaata ctcactggat ttcactaagt tatctatatt aacatctgca gggttattct
     3301 ttggggtcag gctgctatct gcactaaacc agcaccctgt ttagctcatt atttgagaaa
     3361 tatatatgcc agttctgact atctatataa gattagtaat tttcaacaac ttcgagggtc
     3421 atcatgacta agattaggat cattgaaact gcttaccggg atgcccatca atcactactg
     3481 gccacccgta tgaggaccag ggatatgttg cctattgccg aggaaatgga caaagtggga
     3541 tatttctcac tggagtcctg gggaggagca actttcgact cctgcatccg ttacctcaac
     3601 gaggatccat gggaaaggct gagagaactt aaagaacatg tgaccaagac tccactgcag
     3661 atgctactcc ggggtcaga
//
LOCUS       DAOQVW010000107         3670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_28621_length_3670_cov_3.045643, whole genome shotgun sequence.
ACCESSION   DAOQVW010000107 DAOQVW010000000
VERSION     DAOQVW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28621_length_3670_cov_3.045643"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKK85_03280"
     CDS             complement(<1..334)
                     /locus_tag="PKK85_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25070.1"
                     /translation="MDRRKSGGVRQWSGLILIILFLILLAFAIFQRVTLSQWIQNEGF
                     DVLYAVILAIIAGLGIEFFYRKFTKRSRFSRTTMVLKPRKVLARVNLPDGKHLTISKY
                     NRIFGREDF"
     gene            473..1216
                     /locus_tag="PKK85_03285"
     CDS             473..1216
                     /locus_tag="PKK85_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS25071.1"
                     /translation="MLTLPGPGRAMVVTDLHGNLEDFRKVLTLWAKCFNRKCHLVLTG
                     DFIHAMGRANDHSIEIIEEVQYYSTKYDTFHPLLGNHEWATITKILIYKGGVNQNLNF
                     ELLLKETFPDAWEEKLEEYINFFQSLPVAVRTGNGVFISHSGPARVDGIEDIINLTGK
                     GYLDNPVLHDLLWRRDHQKVEVNQFLKRVGCQAMVVGHTPVDGYKLKGNLLILSSSYG
                     KGRKVYLDLDLGKIIKSGRDLEAMIRFFR"
     gene            complement(1238..2050)
                     /locus_tag="PKK85_03290"
     CDS             complement(1238..2050)
                     /locus_tag="PKK85_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNS25072.1"
                     /translation="MSYGNLLTNPLVQVLLATGFTWFMTGLGASTVFLTKKVGRRLLD
                     SSLGFAAGVMISVSFFSLLLPAITVYTPPNVPQWFPVIFGFLLGAVFLGASDRLLPHV
                     HPGCPLDEAEGIRTTWKRNRLLILAMVLHHIPEGLAIGVAFAAASLSFNPYSLMSAIT
                     LAVGMGIHNFPEGMAVAMPLRAEGKSALKSFWYGQLSGVFEPLSAFLGFVLVAVFQPL
                     LPYALGFAAGAMIYVAVEELIPECQRGSHTDLATMALILGFALMMGLNIGFG"
     gene            complement(2125..3276)
                     /locus_tag="PKK85_03295"
     CDS             complement(2125..3276)
                     /locus_tag="PKK85_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS25073.1"
                     /translation="MDRIVKFSHLLRSNGIPASIRSTQTACQVVERFHFNDSQLQDAL
                     AAVYVKDQGQREKFNKLYDDFFYEKKKPLPEEVPKNTKPVKSVVEFPNEGKIQYQFSF
                     KEDPQGELKAVERESTELDYQPPLEEYLDLDEKQDLLHQDISTLNSFQPQLFQVCQEL
                     GRKIATLRSRRMRQSQKLQPDVRRTIRKNLKNGGTLLELVKTRPRIKGGHYYFLCDVS
                     GSCDWISNWFFCLVYAAQNSFRRVRVFDFDNKVVETTSALGEIGLLDAFTRVREMHQK
                     NLMIHGTSNMYQAFRDFQDRAPLNGRSNLIILSDCRDWAGPKEDGQPLSASVIREMSR
                     KSRRVLIFNPEEEKKWDVVDSCVSLYREAGAQVHEVRNLEQMAQMVSVI"
     gene            complement(3312..>3670)
                     /locus_tag="PKK85_03300"
     CDS             complement(3312..>3670)
                     /locus_tag="PKK85_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNS25074.1"
                     /translation="DLLVVLTSNSQRTLLDETRDRCLYLHLDYPDPERELEIVKALVP
                     EAPLPLLKEVVALIQHIRGMDVLKKPSIRATVDWVRSLLTLGEEEPTPEVLSETLGVV
                     VKSPEDRKKVEGHLFK"
BASE COUNT          939 a          961 c          924 g          846 t
ORIGIN      
        1 ggaaatcttc ccggccgaag atacggttgt acttgctgat ggtgaggtgt ttgccatcgg
       61 gcaggttcac ccgggccaac accttacggg gcttcaggac catggtggtc cgggagaacc
      121 ttgatctttt tgtaaactta cggtagaaga attcaatacc cagcccggct atgatggcca
      181 atataaccgc gtaaaggaca tcaaatccct cgttttgaat ccactggctg agggttaccc
      241 tctgaaatat ggcaaaggct agtaaaatta gaaataaaat gatcaggatc agtcctgacc
      301 actgccgaac ccccccagac tttcttcggt ccaagaattt cagcctccca taatacctta
      361 ctattacttt ggacataggc gcctaaaata attaagcaag agaatcctaa gattcccatc
      421 tattaaatat gagaaaaaaa ttattattac tatgaaatac ctggagggcg acttgcttac
      481 acttcctggc ccaggccggg ccatggtggt gactgatctc catggcaacc tggaggattt
      541 caggaaggta ctgaccctgt gggcgaagtg tttcaaccgc aaatgtcact tggtcctcac
      601 cggagacttc atacacgcca tgggtcgggc caacgaccac tccattgaaa tcatcgaaga
      661 agtgcagtat tattccacca aatatgacac cttccacccc ctcctgggaa accatgaatg
      721 ggccaccatc accaagatac tcatctacaa gggcggagtg aaccagaacc ttaactttga
      781 actgctccta aaggagacct tccctgatgc ctgggaggaa aaactagagg aatacataaa
      841 cttcttccaa tccctacccg tggctgtccg caccgggaac ggggtgttca tcagccacag
      901 cggacccgcc agagtagatg gcattgaaga tatcataaac ctaaccggga aaggttacct
      961 ggacaacccg gtactacacg atcttctatg gcgccgggat caccagaagg tggaggtgaa
     1021 ccagttcctg aagagggtgg gctgccaggc catggtggtg ggacacaccc ctgtggatgg
     1081 ctacaaactc aagggaaacc ttttaattct atcatcaagc tatggtaagg gaaggaaggt
     1141 ctacttggat ctggatctgg ggaaaattat aaaaagcggc cgggatctgg aggcaatgat
     1201 cagatttttc cgttaaaaaa aaacccatta ttttttttta gccaaagccg atgttcaaac
     1261 ccatcatcaa agcgaagccc agtatcaggg ccatagtggc cagatcagtg tggctgcccc
     1321 gctggcattc gggtatgagc tcctccactg ccacgtagat catggccccg gctgcaaatc
     1381 ccaatgcata gggtaagagg ggctggaaca cagctactaa tacgaagccc aggaaggcgg
     1441 ataatggttc gaagaccccg gagagttgtc cgtaccagaa actcttcagg gcggattttc
     1501 cctcggcccg taggggcatg gccacagcca taccctctgg gaagttatgg atacccatac
     1561 ccacagctag ggttatggct gacatcaacg agtaagggtt gaagcttaaa gacgcagcag
     1621 caaaggccac ccctatggcc agtccctcag ggatgtggtg taagaccatg gccagtatta
     1681 aaagtcggtt cctcttccag gtggtccgga taccttctgc ttcatccagg gggcagccgg
     1741 ggtgtacgtg gggcagtaaa cggtcactag cacccagaaa cactgcacct aagaggaagc
     1801 cgaatataac tggaaaccac tgcggtacat ttgggggggt gtacactgtg atggcgggta
     1861 acaacaggga gaagaagcta accgagatca taacccctgc tgcaaagccc agggacgagt
     1921 ccagtaaacg tcgccccacc ttttttgtta gaaacacggt ggaagccccc aggccagtca
     1981 tgaaccaggt gaagccggtg gccaggagca cctggaccag ggggttcgta agaaggttac
     2041 cataactcat gagcttacat agttaggaat gtctaataaa agtagtggcc atcccaccca
     2101 gtaggttgga aggggtagtt gatcttagat cacactgacc atctgggcca tctgctccaa
     2161 gttgcgcacc tcatggacct gggcaccagc ctccctgtac aaggagacac agctatccac
     2221 aacatcccac tttttctcct cctcggggtt gaaaatcaaa accctccggg acttcctact
     2281 catctccctg atgacagagg cgctgagggg ctggccatcc tccttgggcc cggcccagtc
     2341 ccggcagtca ctgaggatga tgagattgga tcttccattt aagggggccc ggtcctggaa
     2401 gtcacggaat gcctggtaca tgttggaggt gccatgtatc atcaggttct tctggtgcat
     2461 ctcccgcacc cgggtgaagg cgtccagtaa gccgatttcc cccagggcgg aggtggtctc
     2521 caccaccttg ttgtcaaagt caaagaccct aactcggcgg aaactgttct gagcagcata
     2581 taccaggcag aagaaccagt tactgatcca gtcacaggag ccgctcacat cacacaggaa
     2641 atagtaatgc ccacccttga tccgtggacg ggttttaacc aattccagga gggttccacc
     2701 atttttaagg ttcttacgga tagtgcgacg cacatcaggc tggagtttct gggattgacg
     2761 cattcgccgg ctcctaaggg tagctatctt cctacctaat tcctggcaca cctggaaaag
     2821 ctggggctgg aaggagttca gggtgctaat atcctgatgc agcagatcct gtttctcatc
     2881 caagtccagg tattcctcca gtgggggctg gtaatccaac tctgtggact ctctctccac
     2941 tgccttaagc tccccctgag gatcttcctt aaaggagaac tggtactgaa tcttaccctc
     3001 attggggaat tccaccacag atttaactgg cttagtattt ttgggcacct cctccggtaa
     3061 aggctttttt ttctcataaa agaagtcatc atagagcttg ttgaacttct ccctctgacc
     3121 ctggtcctta acgtagactg ccgccagggc atcctgcaac tgggaatcat tgaaatggaa
     3181 acgttccacc acctggcatg cagtttgggt gctgcggata ctggctggaa ttccattact
     3241 ccttaaaaga tgggaaaatt tcactattct atccatgttc atcccttaaa aatcatctta
     3301 gagacagtcc cctacttgaa aagatggccc tccacctttt tacggtcctc cggggactta
     3361 accaccaccc ccagggtctc ggataggacc tcaggagttg gttcttcttc gcccagggtt
     3421 aagaggctgc gcacccagtc cactgtggcc cgtatggatg gtttcttaag aacatccatc
     3481 ccccgtatgt gctggataag ggccaccact tccttgagga ggggtaaggg tgcctccggt
     3541 accagggcct taactatttc cagttcccgc tctggatcag gatagtccaa gtgcaggtat
     3601 aagcagcgat cccgggtttc gtccaggagg gtgcgctggg agttgctggt tagaactacc
     3661 agcagatcat
//
LOCUS       DAOQVW010000108         3658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_28755_length_3658_cov_3.918401, whole genome shotgun sequence.
ACCESSION   DAOQVW010000108 DAOQVW010000000
VERSION     DAOQVW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28755_length_3658_cov_3.918401"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKK85_03305"
     CDS             complement(<1..691)
                     /locus_tag="PKK85_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNS25075.1"
                     /translation="MLFYAETPLKFAFSKRLIGEEEASLKVQDSQKKVFSILGVPFDG
                     TSTYQPGARFGPMLLRESSYNFEGYNLYLDKKLDTEFYDLGDVQTVPGNFKKTCHQVQ
                     FSVQELVDHGYVPLVIGGDHSISYPVTRALDIGTASVIHLDAHTDLRDDYAGEKYSHA
                     TVMRRISELKPENIIQIGIRSSSPEEVQYARENSLIQFTARDVNKNLDQVVDYLAALE
                     GLTTTGQVVRTG"
     gene            complement(730..1119)
                     /locus_tag="PKK85_03310"
     CDS             complement(730..1119)
                     /locus_tag="PKK85_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HNS25076.1"
                     /translation="MSKKVVEVKTLKVGKYVILDGEASKITSIQTSSPGKHGAAKARV
                     EAVGIFDNQKRSLVKPVDSKIDIPIIDKRAAQVLAIMGSDVQLMDMEDYDTFEVPIPD
                     ELRDQLLEGAEVDYIVAMGNKKLMRIK"
     gene            1317..>1747
                     /locus_tag="PKK85_03315"
     CDS             1317..>1747
                     /locus_tag="PKK85_03315"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNS25077.1"
                     /translation="MKVSMISGRAEGPTKLNSFDNALLDAGIGDVNLITVSSIVSGGT
                     KIVKLPKLEKGAMVNCVLAHAFSDQEGDCVTAVIAVAMADKMGCVVEHSAVNEDPQRV
                     KKEAEAMVRYMMEVRGLNIKEVIMEVVSHEVVNKGSAVAAL"
     assembly_gap    1748..1757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1772..2572
                     /locus_tag="PKK85_03320"
     CDS             1772..2572
                     /locus_tag="PKK85_03320"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HNS25078.1"
                     /translation="MEIIRQVEDVIKPLIGTIKSGKIVKIGADGTPTTLIDVVAEKEV
                     LNALKELEKPLFLISEEIGEVKIGKGPPEAILVVDPLDGTSNAIKKIPAYGISLAVAP
                     CSTKTHKNPNLQEITMGLVKNFATGDIYSGFKGKGAFLNGEKIGPSLVDEISHSSVGA
                     YIYRGDMNRLEPLCKTVRRMRTLGAVAIEISYVADGTYDAFVDMRDNLRVVDVAAAKI
                     IVEESGGIITDRHSHPLHAPLDVLEKTSLIASGNEKLHKNIMDILGGI"
     gene            2572..3408
                     /locus_tag="PKK85_03325"
     CDS             2572..3408
                     /locus_tag="PKK85_03325"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HNS25079.1"
                     /translation="MRIGMVAKVDLEEAVELASKIINFLEEKEVEIVIDQSLALKLNK
                     SQDRALKLKDMEADVVVAVGGDGTILRTQSYISPKKIPLFGINMGTVGFLTEIDSENA
                     FEALEEFLAGNYFVEKRTQLRVWHNNELPSALNEVVLMTRKPAKMLHIQIEVDDEIVE
                     ELRADGLIIATPSGSTAYSMSAGGPIVDPRVGAFIIVPICPFKLGARPTVVANESQIR
                     VKLLKEGKKAIAVIDGQFEEEINYMDEIIFQKSEYCAYFVRFTKNFYRKVREKLIQGG
                     IN"
     gene            3405..>3658
                     /locus_tag="PKK85_03330"
     CDS             3405..>3658
                     /locus_tag="PKK85_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HNS25080.1"
                     /translation="MKTILVVDLTHGGSVIALELSKKKDARVLAWDLYKTLDGEDEDN
                     LKTNGIELVDEDFLSEPVDDLMVIAPVHSKLTVPVELTHH"
BASE COUNT         1069 a          688 c          868 g         1023 t
ORIGIN      
        1 cgccagtacg aacaacttga ccagtagtag tcagaccttc cagagcagcc agataatcca
       61 ccacctgatc caggttcttg ttcacgtccc gggcagtgaa ctgtatcaga ctattttcac
      121 gggcgtattg tacttcttct ggggaagaag agcgtatacc gatttgaata atattttctg
      181 gttttagttc agatatcctc cgcatcactg tggcatggga atacttttca ccggcataat
      241 catcccgcag atcggtgtgg gcatccaaat gtattactga tgctgtaccg atatcaagag
      301 cccgggtaac agggtaactt atgctgtggt cccccccaat gaccagtggg acgtaaccat
      361 gatctaccag ttcctggaca ctaaactgca cttggtggca tgtttttttg aagttgccag
      421 gaacggtctg cacatctccc aggtcataga actctgtatc cagtttttta tccagataca
      481 gattataacc ttcaaagtta taggaagact ccctcaaaag catgggtccg aagcgggctc
      541 ctggttggta cgtggaagta ccgtcaaaag gaacaccaag gatgctaaaa actttttttt
      601 gactatcctg cacttttaaa gatgcttctt cctctccaat taaccttttt gaaaatgcaa
      661 attttagggg agtttcagca taaaaaagca ttagaatgtc ctcaaaaaca ataaataagg
      721 aaaaatgtat tatttaattc gcattaattt tttgtttccc atggctacta tgtaatccac
      781 ttctgcccct tccaggagtt gatcgcgtag ttcgtcgggt atgggaactt caaaggtatc
      841 atagtcttcc atatccatta attggacatc gctgcccatt atagccagaa cctgggctgc
      901 ccgtttgtca attattggga tgtcgatctt ggagtccact ggttttacca ggcttctttt
      961 ttgattgtca aagataccca ccgcttccac acgggccttg gctgcgccat gcttcccggg
     1021 agatgaggtc tgaatactgg tgattttgga tgcttcacca tctaatatga cgtatttgcc
     1081 tactttaaga gttttaactt ctactacctt tttactcatg atattgcctc catgatgcct
     1141 ctgattcaat cagagttaaa tattgatttt tattggtgtt tgaagtgtgg atggctttat
     1201 aacttcagtg aatgagtgaa ccacaccttt aactgggggg tgaaacttaa tagaataccc
     1261 tgctacatat agaattgcct atggtttttt ccctatttaa attttgagtg gttaaaatga
     1321 aagtatccat gatctcagga agggcggaag gtcctactaa gcttaattca tttgacaatg
     1381 cattactgga cgctggaatt ggtgatgtga atcttataac ggtttcaagc atagtatctg
     1441 gtggaaccaa gatagttaaa cttcctaaat tggagaaggg agctatggtt aattgtgtac
     1501 tggctcatgc tttttcagat caggagggag actgcgtaac tgccgttatt gcggtggcta
     1561 tggctgataa aatgggatgt gtggtggaac attctgcagt gaatgaagac ccccaacgag
     1621 taaaaaaaga agcagaagcc atggttcgat atatgatgga agtacgtggc ttaaatatca
     1681 aagaagttat catggaagta gttagccatg aagttgtgaa taagggttca gcagtggcag
     1741 cactcatnnn nnnnnnnggg agcagaccgg tctggaaata atccgacaag tagaagatgt
     1801 gattaaacct ctaatcggaa ctattaaatc aggtaaaatt gttaaaatcg gtgctgatgg
     1861 cactcccacc actctaatcg atgtagtggc ggagaaagaa gttttaaacg ctcttaaaga
     1921 actagaaaag ccgttatttt tgattagtga agagattggt gaggttaaaa ttggtaaagg
     1981 tcctcccgag gccatcttag tggttgatcc tttggatggt accagtaatg ccataaaaaa
     2041 aattccagcc tacggaatat cactggcagt ggccccctgt agcaccaaaa cccataaaaa
     2101 tccaaatctg caggaaataa ccatggggtt agttaagaac ttcgccacgg gagatattta
     2161 ctcgggattt aagggtaaag gagcattctt aaatggagaa aaaattggac catctctggt
     2221 tgatgaaatt tcccactcat cagtgggtgc ttatatttac cgaggagata tgaatcgtct
     2281 ggaaccgcta tgtaagactg tcaggaggat gagaaccttg ggggcggtgg caattgagat
     2341 ctcttacgta gccgatggta cctatgatgc cttcgtggac atgagggata atctgagggt
     2401 ggtggatgtg gctgctgcta aaataattgt agaagaaagt gggggtatta taaccgaccg
     2461 ccatagtcac cctctccatg ctccactaga tgttctagaa aagacttcac tgatagcttc
     2521 tggaaatgaa aagcttcata aaaatattat ggatatttta gggggtattt aatgcggatt
     2581 ggaatggtag caaaggtaga cttggaggaa gccgtggaac ttgccagtaa aatcattaat
     2641 ttcctggaag aaaaggaagt agaaattgtt atagatcaaa gtctggcact caaattaaac
     2701 aagtcccagg atagggcatt aaagctgaag gacatggaag ctgatgtggt ggtggctgtg
     2761 gggggagacg gcaccatact gagaacccag agttatatca gccctaaaaa aatacctctt
     2821 tttgggataa acatgggtac agttggtttt ttaactgaaa ttgattctga aaatgctttt
     2881 gaggctctgg aagagttttt ggcaggaaat tacttcgtgg aaaagaggac ccagctgcgg
     2941 gtatggcaca acaatgagtt accatcagct ttaaatgaag tagtgctcat gactcgtaag
     3001 ccggcaaaga tgctgcacat ccagatagag gtggatgatg aaattgtgga agagttaagg
     3061 gctgatggcc ttataattgc tactccaagc ggttccaccg cctattccat gtccgcagga
     3121 ggacccatcg tggatccccg ggttggggcc tttatcatcg tgcccatctg tccttttaag
     3181 ttgggtgccc gacccacagt ggtggctaac gaaagccaaa tcagggtaaa acttcttaaa
     3241 gaaggaaaaa aggcgattgc agttattgat ggtcaatttg aagaggaaat caactatatg
     3301 gatgaaatta tcttccagaa atctgagtac tgcgcttact ttgtacgttt caccaagaat
     3361 ttctaccgaa aagttcggga aaaactcatt caaggcggaa taaattgaag acgatcttag
     3421 tggtggattt aacccacggt ggctctgtca tagcattgga actctcaaaa aaaaaggatg
     3481 ctagagtact ggcatgggat ctatacaaaa ccttggatgg ggaggatgag gataacttaa
     3541 aaactaatgg tatagagttg gtggatgaag acttcttatc agaacctgtt gatgacttga
     3601 tggttatagc accagtgcac agcaagttaa ctgttccggt agagctaacc catcatca
//
LOCUS       DAOQVW010000109         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_28908_length_3644_cov_3.362775, whole genome shotgun sequence.
ACCESSION   DAOQVW010000109 DAOQVW010000000
VERSION     DAOQVW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28908_length_3644_cov_3.362775"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PKK85_03335"
     CDS             complement(<1..537)
                     /locus_tag="PKK85_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNS25081.1"
                     /translation="MKILRFQIGKKEAIGILKDHLVYEVSSESGTGPQIGPHPELGNF
                     HPLKEVTILPPACPSKVVGVGLNYRDHAVELDMDIPDEPVLFLKPPTTVIGHDGTIIC
                     PPESKQVDYEAELAVIVGQAARKILPEDVSEYIAGYTVLNDVTARDLQRKDGQWTRAK
                     SFDTFCPLGPWIETELDPG"
     gene            complement(553..1413)
                     /gene="hisG"
                     /locus_tag="PKK85_03340"
     CDS             complement(553..1413)
                     /gene="hisG"
                     /locus_tag="PKK85_03340"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNS25082.1"
                     /translation="MKIRIALPSKGRISDPAVKLLEKAGIGLKDAANRKLFSDTYDDD
                     INVMFTRAADIPEFVADGAADMGMTGLDLIREHDAEVEILEDLNFGRAHLVLAAPEDS
                     PINEPGDLADGVIVATEFPHLTQNYLSSRKLDARIVELSGATEIAPFIGVADLITDIT
                     STGTTLQMNHLKIVDNILESSIKLIANPQSYKEKHEKVEAVRTGIRAVLNAEGKKLVM
                     MNVAERFLEDVKKAMPGLTGPTVSQVLSSNDVVAVHAVVDEQEVFKVVNRLKKVGARD
                     ILVVPIERII"
     gene            1538..2827
                     /locus_tag="PKK85_03345"
     CDS             1538..2827
                     /locus_tag="PKK85_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS25083.1"
                     /translation="MESQSILIKNVTMPLSSTKRGSVLIENDLILEVGRDGRDHADVV
                     IDGEGKVLIPGLVNTHTHLSMTLFRGIADDLPLGTWLEDYIWPVEAHLDGEHCYAGAL
                     LGALEMIRTGTTCCSDMYFFMDEIARALDESGLRGVICHGMIDTGIEEKRKNEIKETQ
                     RIIDKCHNTAEGRITVALGPHAPYTCSEELLRWSREKADQEGLQIHMHVSETKDEVQN
                     VLESTQRRPFEYLDSLGLLGDDLLAAHGVWLSNDEISLIKERGVKLSHCPVSNMKLAS
                     GISPITELTRCDVCVSLGTDGAASNNNLDLFQEIKTATLLQKINQMDPTVLPARKVLE
                     MATVDGARALGMENQIGTIEVGKKADLILVDMNAPHLTPPGNHMSHLVYSAGGSDVDT
                     VICNGKILMQNKEILALDHVEVLSLAREAADDLLQRR"
     gene            2838..3527
                     /locus_tag="PKK85_03350"
     CDS             2838..3527
                     /locus_tag="PKK85_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNS25084.1"
                     /translation="MVQNGILVLFDIDGTLVRGARCHYQAFVQAVDKFYQMREDISGI
                     NYAGKTDPQILREVLTMGGVEEKAIQRDFQACLQYMIDYYQKHVHQENIQVLGGVNEL
                     LSQLQSKEVLLGLTTGNLEPIAHAKLGRVGLDDYFPFGGFGSDSEERRGLVKKALERA
                     HDLHHYHGDRIFVVGDTPRDVDAAKPFQIHTLAVATGRYSSQELDQTGADYVLEDLKD
                     LDRILEIIMPD"
BASE COUNT          979 a          848 c          891 g          926 t
ORIGIN      
        1 gcccgggtcc agttcggtct ctatccaggg acctaagggg cagaaagtat cgaaactctt
       61 ggcccgggtc cactgcccat ccttcctttg caaatcccgg gcggttacat cattaagcac
      121 ggtgtaacca gcaatatact cagaaacatc ctctgggaga attttacggg cggcctggcc
      181 cactataaca gctagctccg cttcatagtc cacctgcttg gactcgggtg ggcagatgat
      241 agtcccatca tggccaatga cagtggtggg aggctttaaa aacagcacag gctcatccgg
      301 aatatccata tccaactcca cggcgtggtc acggtaattc aaacccacac caaccacctt
      361 ggagggacaa gctggaggaa gaattgtaac ctccttcaac ggatgaaaat tacctagttc
      421 tggatgagga ccaatttgtg ggcctgttcc ggattcagat gagacttcat agactaaatg
      481 gtcttttaaa atccctattg cctctttttt ccctatttga aatcttaaaa tcttcattta
      541 atccacataa gttcatatta ttctttcaat gggcaccacc agtatatctc gggctccaac
      601 ttttttcaga cggttcacca ctttaaatac ttcctgttca tccaccaccg cgtgaactgc
      661 tacaacgtca ttactggaaa gtacctggga cactgtgggg ccggtgagtc ccggcatggc
      721 cttcttcaca tcctccagaa atctttccgc cacgttcatc atcaccagct tcttaccctc
      781 cgcattcagc acagcccgga taccagttcg tactgcttcc accttttcgt gtttctcctt
      841 gtaactctgg gggttggcta tgagtttgat cgaactttcc agaatgttat ccacaatttt
      901 tagatggttc atctgcagtg tggtgccggt gctggtgatg tctgttataa ggtctgcgac
      961 tccaatgaaa ggggctattt cagtagctcc agaaagttcc acgatacgag cgtctaattt
     1021 tctcgaggaa agataatttt gggtcagatg ggggaattca gtggccacta taactccatc
     1081 tgccaagtct cctggttcat ttatgggaga atcttcaggt gctgccaaaa ccaggtgggc
     1141 tcttccaaag ttaaggtctt ccaggatttc cacctctgca tcatgctccc ggataagatc
     1201 caagccagtc atacccatat cagctgcacc atcggccaca aattcgggaa tatctgcagc
     1261 ccgggtgaac atcacgttta tatcatcatc ataggtatct gagaacaatt tacggttagc
     1321 agcatcttta agtccaatac ctgccttttc caacagtttc acagctgggt cgctgatcct
     1381 gcccttggaa ggtaatgcaa tccttatttt catagataat cctcctttaa aaggtgataa
     1441 ataggtgtaa caagcatttt taatccataa attaagggta agtctaactg ttagctattt
     1501 aatgtttagt tcccttttta tataataggt gtgccccatg gaatcacaga gtatactcat
     1561 aaaaaatgtt acaatgcccc tatccagcac caagagagga tcagtgctca ttgaaaatga
     1621 tctaatcctg gaggtaggtc gggatggaag agatcacgca gatgtggtca tcgatggtga
     1681 aggtaaggtt ctgatcccgg gactggtgaa cacccacacc cacctatcca tgacactctt
     1741 ccggggaata gctgatgatc tgccccttgg gacctggctg gaagactata tctggccggt
     1801 ggaagcccac ttggatgggg agcactgtta cgccggagca ttactagggg ctctggagat
     1861 gatcagaacc ggcaccacct gctgcagtga tatgtacttc ttcatggatg aaatcgcccg
     1921 ggccctggat gaatctggct tgaggggcgt tatctgtcac gggatgatag acacgggcat
     1981 tgaagaaaaa aggaaaaatg agattaagga aacccagcgc attatcgata aatgccataa
     2041 cactgcagaa ggcaggataa ccgtggcatt aggtccccac gctccatata cttgctctga
     2101 ggaacttctg cggtggagtc gagagaaggc agaccaagaa ggcctgcaga tacatatgca
     2161 cgtctccgag accaaggatg aagttcaaaa tgtactggaa tcaacccaaa ggaggccctt
     2221 tgaatatctg gatagtttgg gcctgctggg agatgatctc ttagctgctc atggtgtatg
     2281 gctcagtaac gatgagatca gcctcatcaa agaacgaggg gtgaagttat cccattgtcc
     2341 agttagtaac atgaaattag cgtcaggaat ctccccaata actgaattaa cacggtgtga
     2401 tgtttgtgta tccctgggaa ctgatggtgc tgcttccaac aacaacctgg acctcttcca
     2461 ggagataaaa actgctaccc tactgcagaa gatcaaccag atggatccca ctgttcttcc
     2521 tgcccgtaag gtactggaaa tggccactgt tgatggagcc cgggccctgg gaatggagaa
     2581 tcagattgga accatcgagg tgggtaaaaa ggcggatctg atactggtgg atatgaatgc
     2641 cccccattta actcccccgg gaaatcacat gtcccacctg gtttactctg ccgggggatc
     2701 cgacgtggat actgtaatct gcaatggaaa gatactgatg cagaataaag agattttagc
     2761 actggatcat gtggaggtgt tatccctggc ccgggaagca gcggatgacc ttctgcaaag
     2821 aagataggtg aaaaagtatg gttcaaaatg gaatattggt cctcttcgat attgatggca
     2881 ccctggtccg gggtgcccgc tgccactacc aggcctttgt ccaggctgtg gacaagttct
     2941 atcagatgag ggaggacata agtggtataa attacgccgg aaaaactgac ccccagatac
     3001 tgcgggaagt attgacaatg ggtggtgtgg aagaaaaagc catccaaagg gattttcagg
     3061 cctgtttaca gtacatgata gactactacc aaaagcacgt tcaccaggag aatatacagg
     3121 tactgggtgg agtgaatgaa ctcttgtccc aactccagag caaggaagtg ctcctggggc
     3181 tcaccacagg gaacttggaa cctattgccc atgcaaaact gggccgggtg ggactggatg
     3241 attacttccc attcggtggc tttggaagtg acagtgaaga acgacggggt ttggttaaaa
     3301 aagccctgga gcgggcccat gatttacacc attaccacgg tgatcggata ttcgttgttg
     3361 gagacactcc ccgggatgtg gacgctgcta aacccttcca aattcacacc ttggccgtgg
     3421 ccactggcag gtactcctcc caggaacttg accaaaccgg ggcggattac gttttggaag
     3481 acctgaagga tttagatagg attcttgaaa tcataatgcc agattaaaag gatgaaattc
     3541 taagatatca tcagaattgg aatatttacc cggtccagag gatggtgatg aattaataag
     3601 ggtgtggacg tgttttaaca gggttcatgg gctaatacat gaat
//
LOCUS       DAOQVW010000110         3635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29013_length_3635_cov_3.159218, whole genome shotgun sequence.
ACCESSION   DAOQVW010000110 DAOQVW010000000
VERSION     DAOQVW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29013_length_3635_cov_3.159218"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /gene="rplJ"
                     /locus_tag="PKK85_03355"
     CDS             complement(<1..214)
                     /gene="rplJ"
                     /locus_tag="PKK85_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNS25085.1"
                     /translation="MAHVAQWKKDEVKNLQNLIDSHPVVGIADLADIPAPQMQKMREA
                     LRGNAQLKMSRINLMNLALNQSQKNNI"
     gene            complement(214..852)
                     /locus_tag="PKK85_03360"
     CDS             complement(214..852)
                     /locus_tag="PKK85_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNS25086.1"
                     /translation="MKQEILEAVKKAKKDAKPRNFTQSVDVVITIKDLDVRKPDNRID
                     EEVFLPNGRGKPVKIAFIADGELALLAKNAGADLVINKAELEELGKNRKEAKKIANRH
                     DFFVAQADMMPLVGRFLGPVLGPRKKMPKPVPASAKPEPLMEKLRGTVKVRMKDQPVV
                     QTLVGSQEMGDEEIADNIEVVLGVLDQKLEKGRNQIKSMYVKTTMGPVMRVI"
     gene            complement(948..1436)
                     /locus_tag="PKK85_03365"
     CDS             complement(948..1436)
                     /locus_tag="PKK85_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNS25087.1"
                     /translation="MASETVEILIDGGKATPGPPLGPAIGPLGINMMQVVEQINQKTA
                     DFDGMKVPVKIIVDSATKEFKVEVGTPPTTALIMDELKIEKGSQDPGLEKVADLKMEQ
                     ALKVARMKFDALLSPNYKNAAKEIIGTCVSMGITVEGKDPREVQKEVDEGIYDSVLVQ
                     ES"
     gene            complement(1436..1879)
                     /locus_tag="PKK85_03370"
     CDS             complement(1436..1879)
                     /locus_tag="PKK85_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HNS25088.1"
                     /translation="MIYAIRTLVGQEKNVARVIARNVKNSGIGVSSILVPESLRGYIL
                     VESTTKLDMQNPAFKVPHMKGSIEGEIPYEEIKNFLNPEPILSSIQKGSIVELISGPF
                     KGEKAKVVRIDESKEDVVLELIEAAVPIPVTVKGDQIRLIQKEAD"
     gene            complement(1913..2095)
                     /locus_tag="PKK85_03375"
     CDS             complement(1913..2095)
                     /locus_tag="PKK85_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HNS25089.1"
                     /translation="MNLNKESVVSFLKQCQRVLHVSKKPDRDEYLNVSKITGIGIIII
                     GVVGFIISIIAQLLGA"
     gene            complement(2325..3413)
                     /gene="ftsZ"
                     /locus_tag="PKK85_03380"
     CDS             complement(2325..3413)
                     /gene="ftsZ"
                     /locus_tag="PKK85_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNS25090.1"
                     /translation="MSKRGEYDTSIDHELEDIIKRSRAKILVIGAGGAGNNTVSRLTD
                     IGIEGAKTISLNTDAQDLFYSTADQKILIGKDTCGGLGAGGIPDVGEESAEESEEDIK
                     AKLEGADMVFVTCGLGGGTGTGSAPVISKLAKKIGALTIAVATMPFSAEGLRRRENAE
                     SGLEKLQNAADTVIVIPNDKLLEVAPNLPINKAFMVADELLGRAVKGITELITKPGLV
                     SLDFADIRSIMKGSGMAMIGMGESDSGDRAIESVHEALNSPLLDLDISNAKGALVNIS
                     GSSDLTLQEAENVVQIVADELDPDANIIWGTQIQEDLQNIIRTTIVVAGVNSPHIFGV
                     PGEGEFVGERQQEKAPESSLEEFIDGVF"
BASE COUNT          854 a          888 c          716 g         1177 t
ORIGIN      
        1 ttatattatt cttctgggac tgattcagag ccagattcat caaatttatc cgggacatct
       61 tcagctgagc attgccccgc agggcctccc tcattttctg catctgaggg gcgggtatat
      121 ccgctagatc agctattccc accaccggat gactatcgat gaggttctga agatttttta
      181 cttcatcctt tttccactgt gcaacgtgtg ccattagatc accctcatta ctggccccat
      241 ggtggttttc acgtacatgg acttgatctg gtttcgtcct ttctccagtt tctggtccag
      301 tacacccagc acaacttcaa tgttatcggc aatttcttca tctcccatct cctgggagcc
      361 tactagagtt tgcaccactg gttgatcctt cattctcacc tttacggtgc ctctcagttt
      421 ctccataagg ggttcaggct tagctgaagc tggaactggt ttgggcattt tcttccgggg
      481 ccctaaaaca ggtcccaaga acctaccaac caggggcatc atatctgctt gggccacgaa
      541 gaaatcatga cggttagcaa tcttttttgc ctctttacgg ttcttaccta attcttccag
      601 ttcggccttg ttgatcacca gatccgcccc ggcatttttg gccagcaggg ccagttcacc
      661 gtcggcaata aaggcgatct taaccggttt tcctcttcca ttggggagaa acacttcttc
      721 gtctatgcgg ttatctggct ttctcacgtc taaatccttg atggtgatta ctacatcaac
      781 ggattgtgtg aagtttctcg gctttgcatc ttttttagcc ttcttcaccg cttctaagat
      841 ctcttgtttc atccaattcc tccatgaact tctcgggtga aaagttcaac gtatttctga
      901 tgtgaatgcc attttctttc aatgtctcat gtaatgatca atgaaattta cgattcctgc
      961 accaatacac tgtcatagat cccttcatcc acttctttct ggacttcacg gggatcttta
     1021 ccctctacgg taattcccat gctcacgcag gtaccaatga tttccttagc cgcgttttta
     1081 taatttggtg acaggagggc atcgaatttc attcgagcaa ccttaagggc ctgttccatt
     1141 ttaaggtcag ctaccttttc cagcccggga tcctgggatc ctttttcaat tttcaattca
     1201 tccatgatca gagcggtggt gggtggtgta cccacttcta ctttgaattc cttggtagcg
     1261 gagtctacaa tgatcttcac tggcaccttc ataccatcaa agtctgcagt tttttggttg
     1321 atctgttcca ctacttgcat catattgatg cccaggggtc caatagcggg acctaagggt
     1381 ggtcctggag tggcttttcc gccgtcaata agaatttcta cggtttcact tgccattagt
     1441 ctgcctcctt ctgtattaat cttatttgat cacctttaac agtaactggg atgggaactg
     1501 ctgcttcaat cagttccaga actacatctt ctttagattc atctattcta actactttgg
     1561 ccttttctcc tttaaaaggc ccagatatga gttctacaat gcttcccttc tggatggaag
     1621 atagtatagg ttcgggattt aagaagtttt ttatctcctc gtagggtatt tccccttcga
     1681 tggaaccctt catatgaggc accttgaagg ccgggttttg catgtccagc ttggtggtgg
     1741 actctaccag aatatatccc ctgagacttt ctggaaccag tatagaacta actccaatcc
     1801 cactattttt aacgttcctg gcaattacac gggccacgtt tttttcttga cccaccaggg
     1861 ttcttattgc atagatcaaa ataccactac ccaaaaatat tacactggaa cattatgccc
     1921 ctaagagctg ggcaattata ctgatgataa aacctaccac gcctatgatt atgattccaa
     1981 ttccagttat cttggaaaca ttgagatact catcacggtc tggttttttg gatacatgta
     2041 gtaccctctg gcactgtttt agaaaactga ccacagattc tttgttcaaa ttcataaaaa
     2101 tacccacttc atgcgatttc tattttagaa gaaagtatgt aaaatctaga tagaacaaaa
     2161 taagttattg tgtgaggggt ttataaacct ttccatgtag ggggtaggcg gaaagggtcg
     2221 atggaaccac ctatagtgga acccctcaat ttataacaat caggggatgg atttggtgcc
     2281 tttttatcgg ctgacaaagt ccgatgggaa ttacaataga tttattaaaa aacaccatcg
     2341 atgaattctt ctaaggaaga ttcaggggct ttttcctgct gcctttcccc cacgaattct
     2401 ccttcaccag gcacaccaaa tatgtgtggt gagtttactc cggccaccac tatagtggtg
     2461 cggatgatgt tctgtaagtc ttcctgaatc tgggtacccc agattatgtt ggcatctgga
     2521 tctagttcat cagccacaat ctgcaccacg ttttcggctt cctgcagggt taaatctgag
     2581 cttccagata tgttgaccaa tgctcctttg gcattggaga tgtccagatc caagagggga
     2641 ctgtttagtg cttcgtggac ggattctatg gcccgatcac cggaatctga ctcccccatt
     2701 ccaatcatgg ccattccgga tcccttcatg atgctgcgga tgtctgcaaa gtccagactc
     2761 accagccctg gtttggttat gagttcagtg attcctttca ctgctcggcc cagaagttca
     2821 tcagccacca tgaaagcctt gtttatggga agattgggtg ctacttccag gagtttatcg
     2881 ttggggatca cgatgactgt atcagctgcg ttttgcaact tttccaggcc actttctgca
     2941 ttttctcttc tacgaagacc ctcagcactg aaaggcatag ttgccacagc aatggtgagt
     3001 gcaccaatct ttttagccaa tttggaaata actggagcag aaccggtacc ggttccacca
     3061 cctagaccac aagtcacgaa gaccatgtcc gctccttcta gtttggcttt gatatcttct
     3121 tcactttcct cagcgctttc ttcaccaaca tctggtatgc ctccagcacc taaacctcca
     3181 caggtatctt tacctatgag tatcttctgg tcagctgtag aatagaaaag atcttgagca
     3241 tcagtgttca gagaaatggt tttagctcct tcgatcccaa tgtcagtgag tcgggaaaca
     3301 gtgttgtttc cagcgccccc tgcacctatc actaaaattt tggcccgact tcgctttatt
     3361 atatcttcca gttcatggtc aatactggtg tcgtattcac cacgcttgct aatgtctgat
     3421 ctattccttc tattttcaga ttcttttatg gtattgttta tgagagattt caattcctac
     3481 ccccacttgt agagttgtta tacttaattg tgttattgtt tttacttata tttaaaagca
     3541 acggtttttt gacctattat tttccttcca acatacaatt ttcaactctt atgtgcagat
     3601 catcaccatc ataggaccat gaagtcattg tgcac
//
LOCUS       DAOQVW010000111         3634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29019_length_3634_cov_3.261246, whole genome shotgun sequence.
ACCESSION   DAOQVW010000111 DAOQVW010000000
VERSION     DAOQVW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29019_length_3634_cov_3.261246"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKK85_03385"
     CDS             <1..323
                     /locus_tag="PKK85_03385"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HNS25091.1"
                     /translation="DYWFFETAVRLHLSGRGKPYHGIKPAGYNRRPALTLAEEALKKD
                     NSLDLINFMVSFMQEDIQTRFEDVLSKKDYELKDIESGRDYISSMQDFIRYLDKLYEF
                     MEQG"
     gene            338..661
                     /locus_tag="PKK85_03390"
     CDS             338..661
                     /locus_tag="PKK85_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNS25092.1"
                     /translation="MSKELESIPIRLDDLIEYQEGSVVSREIIRKETGTVSIFAFDIG
                     EGLSEHTAPFDAMVQVIDGRAEIIIAGNKNLVDEGDMIIMPANIPHALHAVEKFKMVL
                     TMIRS"
     gene            complement(720..1529)
                     /gene="cas4"
                     /locus_tag="PKK85_03395"
     CDS             complement(720..1529)
                     /gene="cas4"
                     /locus_tag="PKK85_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNS25093.1"
                     /translation="MISASSISEFLYCPLKAYLKYVQGYEISSETYLIGKLTHELFRG
                     FNEITIRNLWSLDPTMTLNEISSQLWDDVPSLIKKIIYKKDYRDIIDGETVKEIYETL
                     EDQFKLDAFQLVFKSRKMLNMGMSGDEIADILFPPAMVEFWMESRSLGLRGKVDRIEI
                     EDGAYFPVKIITGTPPVKGVWKSHAMQLVAYAILMEETFQKEIRVGFVDYVLVGERKP
                     VMISQEMREEFYNTFNEMESMMLEGYVPEATPSGKKCKKCEYREFCEYGDG"
     gene            complement(1614..2897)
                     /locus_tag="PKK85_03400"
     CDS             complement(1614..2897)
                     /locus_tag="PKK85_03400"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNS25094.1"
                     /translation="MKKTVNIGLIGFGTIGSGVVETFNRNLPLIEDKVGANLVLRKIV
                     DLDIVTDRGVQVDPQLLSTNVDDILEDPEMDIVIELIGGYQPALNFILKAMKNGKHVV
                     TANKALLAKHWEEIMETARENQVRVCFEASVGGGIPLLEPLNDSLAANQIQSIYGIIN
                     GTANYILTKMSEEGRDFEEVLKEAQKLGYAEQDPTFDVEGVDTAQKLIILTILGFGIF
                     IEQEKLHVEGISMITPQDIQFASRELGCVIKLLAISQLVDGSLEVRVHPTLLPEDHLL
                     AAVKGVFNGIYIVGDVVGPVMMYGPGAGMLPTASAVVSDCMDIIGAPEKPLYYGPQKS
                     NVDSIKPMSEVESRYYLRISAIDKPGVLHAISGILSGLNISIESVNQEKRDEGHEVPI
                     FIVTHHAKEQNMLQAVEMIENLESVTKDMVFIRIL"
     gene            complement(2929..>3634)
                     /locus_tag="PKK85_03405"
     CDS             complement(2929..>3634)
                     /locus_tag="PKK85_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNS25095.1"
                     /translation="DQLLQKVVIVAEFFRDEANIPPSHMGISGSILPGLYDPGVSDID
                     FVVYGLKNHERAMEAFFQIKDNPNYPLDAIGEDYWLKLYEKRIKDSSLSFQEFQWYEN
                     RKNNRGVVSGTLFDILATRDWSEIEGIYGDEVYEALGDSEIECTVKNAIASFDNPAIY
                     EVEDVKILNGPTAPITQVASYTHTYAGQAVAGERILVKGKLEKVTGKKTVHRLIVGTT
                     RESVGEYIKLKSLEIT"
BASE COUNT         1030 a          812 c          673 g         1119 t
ORIGIN      
        1 cggactactg gttcttcgaa actgcagtcc gactccacct ctccggccga ggaaaaccat
       61 accatggaat caaacccgca ggatacaacc ggcgccctgc actgactctg gctgaagaag
      121 ctctaaaaaa agataattca ctggatctca taaattttat ggtttcattc atgcaagagg
      181 atatacaaac acgtttcgag gatgttcttt ccaaaaaaga ttatgaacta aaagatatag
      241 aatcaggacg ggactacata tcatccatgc aggattttat ccggtacttg gataaattat
      301 atgaatttat ggaacaagga taaatagagg tgcgttaatg tctaaagaac tagaatccat
      361 accaataaga ttagacgatt taatagaata tcaggaaggt tcagtggtta gtagagaaat
      421 aataagaaaa gaaaccggaa ctgtatccat attcgcattt gacataggtg aaggtttaag
      481 tgaacacaca gcccccttcg atgccatggt acaagtaatc gatggccgag cagaaattat
      541 catcgctggt aacaaaaact tagtggatga gggggacatg atcatcatgc ccgccaacat
      601 cccccatgct cttcatgcgg tggagaagtt caagatggtt ctaaccatga tccgttccta
      661 gtggggattg tggaagccgc ctcggtgggg ggggggtggt ctatacttat tttttttctt
      721 taaccatctc catattcaca aaattcccta tattcacatt ttttacattt tttaccactg
      781 ggggtggctt ctggaacgta tccttctaac atcatgcttt ccatttcatt gaaggtattg
      841 taaaactctt ctctcatttc ctgacttatc ataactggtt ttctctcccc gactagcacg
      901 tagtctacaa atcctaccct aatttctttc tgaaaggttt cctccattaa tatggcatat
      961 gccactaatt gcattgcgtg tgacttccaa actcctttaa ctggtggagt tccggttatt
     1021 attttcactg gaaaataagc accgtcttca atttctattc ggtctacctt tccacgcagt
     1081 ccaagtgaac gactttccat ccaaaattct accatggcgg gagggaatag gatatctgct
     1141 atttcatccc cactcattcc catattcaac attttgcgtg atttgaatac cagttggaag
     1201 gcgtccaatt tgaactggtc ttccaatgtt tcatatatct ctttaactgt ctctccatct
     1261 atgatgtctc gataatcttt tttataaatg atttttttga ttaaggaggg aacatcatcc
     1321 caaagctgac tggatatttc gtttaaggtc atcgtggggt ccaatgacca aagattacgt
     1381 atggttattt cattaaaacc tctaaaaagt tcatgtgtta gttttccaat taaataggtt
     1441 tcgctggata tttcatatcc ctgcacatac tttaaatagg ctttcaaagg acagtaaagg
     1501 aattcggata tggagcttgc actgatcaat caatcaccca cctattgaca taaaaattag
     1561 taataaatta ttacttaata ttaataaatc ttacgatggt aataatattt ggtctagaga
     1621 attctgataa aaaccatatc tttcgtgact gattccaagt tttcaatcat ttccacggct
     1681 tgaagcatat tctgctcttt agcgtgatga gtaactatga atataggaac ttcatggcct
     1741 tcgtctcttt tttcctggtt cacagactct atacttatat tcagaccact taaaataccc
     1801 gatatggcgt gtagaacccc tggcttgtca atggctgata tccgaagata gtaacgggat
     1861 tcaacttcag acatgggttt tatactatct acattgcttt tttgcggccc ataatatagt
     1921 ggtttctcag gagcccctat gatatccatg caatctgaga ctacagcact ggcagttggt
     1981 aacattcctg cgccggggcc gtacatcatc actggcccca ccacatcacc tacaatgtaa
     2041 attccgttga acactccttt aaccgcggcc aaaagatggt cttctggtaa cagggtgggg
     2101 tgcaccctaa cctccagact tccatccacc aactgggata tggccagcag cttaataaca
     2161 caacccaatt ctcgagaggc gaattggatg tcctgggggg tgatcatact tataccctcc
     2221 acatggagtt tttcctgctc tatgaaaatc ccaaacccaa gaatggttag aataatgagt
     2281 ttttgggcgg tgtcaactcc ttccacatca aaagtagggt cctgttctgc ataaccaagt
     2341 ttttgagctt ctttaagaac ttcttcaaaa tcacgtcctt cttcactcat tttggtcaga
     2401 atgtaattgg cggtaccgtt aataattcca taaatagatt ggatctggtt agcagccaaa
     2461 ctatcattga ggggctctaa aagtgggatt ccaccaccta cactagcttc aaaacacacc
     2521 ctaacttggt tttcacgggc agtttccata atttcttccc agtgtttagc tagaagggct
     2581 ttgttggcgg ttaccacatg tttaccattc ttcatggctt tgagaatgaa attcagagcg
     2641 ggttgatagc ccccaattag ttcgataact atatccattt ctggatcttc cagtatgtca
     2701 tcaacgttgg tggaaagtag ttgaggatca acttgcacac ctcggtcagt aacgatatcc
     2761 agatccacga ttttcctcag caccaaattt gcgccaactt tgtcttctat taggggtaaa
     2821 tttcgattaa aagtttctac tactccgctc ccaatagttc caaaaccaat aagccctata
     2881 ttcacggttt ttttcataag catcatctcg taaaatagca ttatcttgct aggttatctc
     2941 caaacttttt aacttaatat actctcccac ggattcacgg gtggtaccca ctatcaaacg
     3001 atggactgtt tttttaccag tgaccttttc caattttcct ttaaccagaa tcctctcacc
     3061 agccacggct tgaccagcat aagtatgggt gtatgaagct acttgggtta tgggggctgt
     3121 tggtccattc aatattttca catcctccac ctcgtagatg gccgggttgt caaaggaggc
     3181 aatggcgttt ttaacagtac actcaatttc agagtctccc agtgcttcgt aaacttcatc
     3241 tccataaata ccttctattt ctgaccagtc tctggtggcc agaatgtcaa agagcgttcc
     3301 agaaaccaca ccccgattgt ttttacgatt ttcataccac tggaactcct ggaaactaag
     3361 tgaagaatct ttgatccttt tttcatacag ttttagccaa taatcctctc caatggcatc
     3421 taatggataa ttaggattat ctttaatttg aaaaaaagct tccatagctc tttcatgatt
     3481 tttaaggcca tatacaacaa agtcgatgtc tgaaacacct ggatcatata acccgggcaa
     3541 gatagagccg gaaataccca tatgtgatgg gggaatattg gcttcatccc gaaagaattc
     3601 cgctactatc accaccttct gaaggagctg atcc
//
LOCUS       DAOQVW010000112         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29252_length_3614_cov_3.692891, whole genome shotgun sequence.
ACCESSION   DAOQVW010000112 DAOQVW010000000
VERSION     DAOQVW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29252_length_3614_cov_3.692891"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /gene="hacA"
                     /locus_tag="PKK85_03410"
     CDS             complement(<1..1200)
                     /gene="hacA"
                     /locus_tag="PKK85_03410"
                     /EC_number="4.2.1.114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitase large subunit"
                     /protein_id="HNS25096.1"
                     /translation="MPRTMAEKILAKAAGIEEIDAGKIVMANVDLAMTHDLTGPLSVA
                     AFKKIGENEVWDPEKIVVIFDHQVPADSLEAAQNHLIMRKFVKEQGIKNFYDVGEGVC
                     HQVLPEKGHVVPGEVVVGTDSHTCTHGALGAFATGIGSTDMAMVFATGKLWFKVPETL
                     KFEINGKLATNVCSKDVILNIIGQVSVDGASYQACEFGGETVSDMSVSDRMVLCNMAI
                     EMGGKTGLVEPDEKVIKYVESRSNKNYQVFTSDEDAESLTTLHVDVTDLEPQIACPHN
                     VDNVKPVSDVEGTPLDQIFLGSCTNGRLDDMRVAAEILKGKEVSPRVRMLVIPASREV
                     YRNALEEGLMNIFVDAGALVCNPCCGPCLGGHVGLLGPGEVSLSTSNRNFQGRQGSPE
                     AEVYLSSA"
     gene            complement(1278..1703)
                     /locus_tag="PKK85_03415"
     CDS             complement(1278..1703)
                     /locus_tag="PKK85_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNS25097.1"
                     /translation="MNEELELVEAMDDLSELIKKFQTQLLSGELKQYTLRQLYYIELI
                     NKYEGISVSEISKKLDIKKSTVSIAINQLMDLEIVTKIRSEDDKRFYFLQLTPKGKKI
                     MQMHMQVHKNTIKKILKILTLEEVENFIQIVNKIKMSNL"
     gene            1955..>3614
                     /locus_tag="PKK85_03420"
     CDS             1955..>3614
                     /locus_tag="PKK85_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNS25098.1"
                     /translation="MREISEEIKQEYEKIKDKISYEDFLEQMEIRAQDYEGVSFMSEL
                     DLARVIVGEYVEEKNEPLVQESKNPKLSELETGHHNLTLVGRVMKMSNIKKFTSRKGK
                     EGRLVNIILGDETGSMRVVFWTENIKLLTKNDVQEGDVINLKGVEIKQGYREDEAHLN
                     ARSELKKLPSKEFTHLPSPETGITRLGDVQGDMEVNVIARIIRIPRIRTFYRNGSEGK
                     VASLELQDESGKMTFTLWNRDTDLIESLELQEGDAVKIMGAQSRVRNGEVSLSHSWIG
                     RIEKGDFDVPEYSENVLKIGDAREMRDVTIIGVASKVYDEIIFERNDGSTGRVKSLEL
                     EDDTGVIRVTLWNEDTALEIKKGDIIKIIGGNIEFDDYSTAGYRINTNWNSKILINPP
                     IDAKLKEILQECGKYLKPHKIGELNQIEDEGDEVDVVGRVVNLYETNEFQREDGSKGV
                     VRSAELADETGVVRVSLWDEKAEGHLNEGDAVKIENARTRLGNYNVELNVGKTSRILK
                     PSPEETETLPSLKEVADMLYQDKKITELIEGDREARIMGRILSLY"
BASE COUNT         1119 a          723 c          773 g          999 t
ORIGIN      
        1 cgctgaactt aggtaaactt ccgcttcagg gcttccctgc cgcccctgga agttgcggtt
       61 ggaagtggaa aggctgactt cacccggccc taagaggccc acatgtccac ccaggcaagg
      121 tccgcagcag ggattgcata ccagggcgcc ggcatctaca aatatgttca tcaggccttc
      181 ttccagtgcg ttgcgataga cttctcggga agctggtata accagcatgc gcacccttgg
      241 ggaaacctcc tttcccttga ggatctcagc tgccacacgc atatcatcta agcgcccgtt
      301 tgtacaggaa cctaggaata tttggtcaag gggagttcct tcaacatcac ttactggttt
      361 tacattgtcc acgttatggg ggcaggcaat ctgaggctcc aaatcggtta catccacatg
      421 gagggtggtc agggattctg catcttcatc actagtaaat acctggtagt tcttatttga
      481 tctactttca acatatttaa tgactttttc atcaggttct accaggcctg ttttaccccc
      541 catttctatg gccatgttac atagcaccat acgatctgaa actgacatat cactgacagt
      601 ttccccacca aactcacaag cctgataact agcgccgtca acacttacct gacctattat
      661 atttaaaatc acgtcctttg aacaaacatt tgtagctaat tttccgttaa tttcgaattt
      721 caatgtttca gggaccttaa accatagttt accggttgca aataccatgg ccatatctgt
      781 cgatcctatt ccagttgcaa atgcccccaa cgccccgtgg gtacaagtat gagagtctgt
      841 tcccaccacc acctcacctg ggactacgtg tcctttctct ggaagtacct gatggcagac
      901 tccttctccc acatcataaa aattctttat tccctgttct tttacaaatt tacgcattat
      961 caaatgattt tgggcggctt ccagtgaatc tgccggtact tgatggtcaa agatcaccac
     1021 aattttttca ggatcccaaa cttcattctc cccaatcttt ttgaatgccg ctacggataa
     1081 tggtcctgtc agatcatgag tcatggccaa gtctacattg gccataacaa tttttccggc
     1141 gtcaatttct tctattcccg ctgctttagc tagtattttt tctgccatag ttctaggcat
     1201 tggcactcct ccatccattg taaaacccta taaatcaatt tatactatcg atgttacaca
     1261 atatagctat aaatccatca taaattcgac attttgattt tattcactat ctggataaaa
     1321 ttttcgactt cttctaatgt taaaatcttt aaaatctttt ttattgtgtt tttatgcact
     1381 tgcatgtgca tttgcattat ttttttacct tttggtgtta gctgtaaaaa ataaaagcgt
     1441 ttatcatcct cagatcgaat ttttgttact atttcaaggt ccattaactg gttgatggca
     1501 atggaaactg tggatttctt tatatccagt ttttttgata tttcagaaac gctaatgcct
     1561 tcatacttat taattagctc tatataatac aactgccgta gtgtgtactg ttttagctct
     1621 ccactaagaa gttgagtttg gaatttttta attaattcac ttaaatcatc catagcctct
     1681 accagttcca attcctcatt catgggcccc atcctcacgt aagaagtaat agtcacccca
     1741 tagccaacaa atatatagtt ttatctaact aactattata aaaagatatc cataaaaagt
     1801 tccataggct cgttgtttag tagggtcagt tagtgggatc ttcaggattt tgtaataaga
     1861 ataactataa atagtaatac taatatgatt aattagtacc gttaggcaat agatactatt
     1921 atcgaagcga gatattttgg tgagggaacc caaaatgagg gaaattagcg aagaaataaa
     1981 gcaggaatat gagaaaatca aggataaaat ctcatatgaa gattttttag aacagatgga
     2041 gattagagct caggattatg agggagtaag ctttatgagc gagctagacc tcgccagggt
     2101 aattgtagga gaatatgttg aagaaaaaaa tgagcccctc gttcaggaat ctaaaaatcc
     2161 taaattatca gagctggaga ccgggcatca taacctaacc ctggttggta gagttatgaa
     2221 aatgtccaac atcaaaaaat tcaccagtag gaaaggtaaa gaaggcaggc ttgtcaacat
     2281 catccttggt gatgaaaccg ggagcatgag ggtggttttc tggacagaaa acattaaact
     2341 actcaccaag aatgacgtcc aggaagggga tgtgattaat ctgaaaggag tggaaataaa
     2401 gcaaggatat cgagaagatg aagcccactt gaacgccaga tcagagctga aaaaactccc
     2461 ttccaaagaa tttacccact taccttcacc cgaaacaggg ataaccaggt taggtgatgt
     2521 tcagggagat atggaagtga acgtcattgc ccggataatt agaatacccc ggattagaac
     2581 cttttaccgt aatggatccg aaggtaaagt agcttccctg gaacttcagg atgaaagtgg
     2641 aaaaatgacc ttcaccctct ggaaccgaga taccgatctt atcgaaagcc ttgagcttca
     2701 ggaaggagat gccgtgaaaa taatgggggc acaaagtcgg gttagaaatg gagaagtttc
     2761 attgagccat tcctggatag gtcgtatcga aaaaggagat tttgatgtac cagaatatag
     2821 tgaaaacgtg ctcaaaatag gggatgcccg tgaaatgaga gatgtgacta ttataggagt
     2881 ggccagtaag gtttatgacg agattatctt tgaaagaaat gatggcagta ccgggcgagt
     2941 gaaatcattg gaactggaag atgataccgg agttatcagg gtgacgctgt ggaacgaaga
     3001 taccgctctg gagattaaaa aaggagatat tatcaaaatc atcggaggaa atattgagtt
     3061 tgacgattac tccactgcag gataccggat aaacaccaat tggaacagta agatactaat
     3121 caatccacca atagatgcca aactcaaaga aatactccaa gagtgcggta aatacctcaa
     3181 accacataaa attggtgaat taaaccagat agaggatgaa ggagatgagg tggatgttgt
     3241 ggggcgagtg gtaaatctgt atgaaaccaa cgagttccag agggaagacg gtagcaaggg
     3301 agtggtccgt agtgcagagt tggcagacga aaccggagtg gtgcgggtat cattgtggga
     3361 tgaaaaagca gagggacatc tgaatgaagg agatgctgtg aagattgaaa atgccagaac
     3421 acgactggga aattacaacg tagaactgaa cgttggtaaa acatccagaa tattgaaacc
     3481 cagcccagaa gaaacagaaa cgttgccttc tcttaaagaa gttgcagaca tgctttatca
     3541 ggataaaaag attactgagt tgattgaagg ggatcgtgaa gcgcgaataa tgggcagaat
     3601 cctcagtctc tatg
//
LOCUS       DAOQVW010000113         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29255_length_3614_cov_3.517842, whole genome shotgun sequence.
ACCESSION   DAOQVW010000113 DAOQVW010000000
VERSION     DAOQVW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29255_length_3614_cov_3.517842"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKK85_03425"
     CDS             complement(<1..312)
                     /locus_tag="PKK85_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNS25099.1"
                     /translation="MGKKIWQNIPLTTVLIALPIFLFFISFLIGRYPLSPPEVILALL
                     SKVLPVKAVSPAVYSIVWEIRLPRIMAALLVGAALSIAGASFQGIFQNPLVSPDILGV
                     TS"
     gene            complement(340..1410)
                     /locus_tag="PKK85_03430"
     CDS             complement(340..1410)
                     /locus_tag="PKK85_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNS25100.1"
                     /translation="MDNKNKTIFAIIVVIVIVGTLGAYVTYYTPITGGTTQITDMVGR
                     NVTVPDQITRVVATSPPTTNLVYMLAPEKLAGWNFKPTGQYMNPKYKNLTEVGGWFGK
                     QTGNYETFISINPDIVLEGSSPLANSNDTIIERQNKFGSIPVVAVLDVSNVTKFTPSI
                     KFVGQVLGSQDKANKLVLFYEKVYNQVNNTASQIPDEEKKKVYYAEGAEGLQTEPTGS
                     QHSQLIELAGGINIANVPLKQGMGMSDVSIEQVLKWDPDVILVGDPTFYKKVYNDTKW
                     QNVKAVKEKQVFLIPQDPFNWFDRPPGVNIILGIPWTAKTIYPDKFQDLDMNSLTKEF
                     YSEFYHYQLTDDQLNNLLNPQP"
     gene            complement(1435..2190)
                     /locus_tag="PKK85_03435"
     CDS             complement(1435..2190)
                     /locus_tag="PKK85_03435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS25101.1"
                     /translation="MPDKNNSESWDNIAPKFNKWMEKDDYPEKVLSRIKIEPSDSILD
                     IGCGNGVITIPLAKKARKVTAMDISQKMLDILTVKAEKSKINNINIVNKRLEDISTAE
                     IGKHDVIVASRSLNGVSNIEKELKKINQIAQKYVYITIWGADSRKFEKDMAHLLGRES
                     YSHPDYIYVYNILHDMGVYANVEMLESNTRDYSTVDEALERLKWRIGDLNKFEESVLR
                     EYLERNMIKTHEGNLTYSQGRADWVLIWWKKTV"
     gene            complement(3094..3303)
                     /locus_tag="PKK85_03440"
     CDS             complement(3094..3303)
                     /locus_tag="PKK85_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNS25102.1"
                     /translation="MKMSARNMLKGKVEGVEAGAVMANVKIKIEAPDIITAIITKDSV
                     QDLDIKVGDAVVAIIKSTEVMVGKE"
     gene            3406..>3614
                     /locus_tag="PKK85_03445"
     CDS             3406..>3614
                     /locus_tag="PKK85_03445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HNS25103.1"
                     /translation="MNSVKYEPIGTIHSPYKDLHGMPIQPIGAQGVKGKIELKNEYKE
                     GLKDLQGFSHIILLYHLHLCNGHSL"
BASE COUNT         1077 a          715 c          595 g         1227 t
ORIGIN      
        1 tgaggtcact cctaatatgt ccggagaaac taatgggttt tgaaatattc cctggaatga
       61 agctccggcg attgacagtg ctgctcctac caaaagagca gccatgatcc gaggtaatct
      121 tatctcccaa acaatagaat aaactgctgg agatacagct ttcactggta aaacctttga
      181 taataatgct aaaattactt ctggtggaga taggggatat ctcccaatga gaaaagaaat
      241 aaaaaataaa aaaataggca gagctattag aacagtagta agaggtatat tctgccagat
      301 tttcttaccc atagacattt tccattacct attcatcaat tatggctgag gatttagcag
      361 attgttcaac tggtcatcag ttaactggta atggtagaat tcggagtaaa actccttagt
      421 caagctgttc atgtccagat cctgaaattt atccggataa atggtctttg cggtccaggg
      481 tattcctaga attatgttaa caccgggcgg ccgatcaaac cagttaaatg ggtcctgagg
      541 tattaaaaag acctgttttt ccttaactgc cttgacattc tgccatttgg tgtcattgta
      601 gactttttta taaaaggtag ggtcacctac cagtatcaca tcggggtccc acttcaatac
      661 ttgttcaatg gaaacatcag acattcccat tccctgtttt agaggaacat ttgctatgtt
      721 tataccaccc gccagctcaa ttagttgtga atgctgggaa cctgtaggtt cagtttgtaa
      781 accttctgca ccctcggcat agtacacctt cttcttctct tcatctggaa tttgggatgc
      841 tgtgttgttc acttgattat acactttttc ataaaataaa acgagtttgt tggctttatc
      901 ctgagatcca agaacttgtc ccacgaattt tatggatggg gtaaattttg tgacattgga
      961 aacatccaga actgccacaa ctggaataga accaaattta ttctgacgct cgattattgt
     1021 gtcattagaa ttagctaggg ggcttgaacc ttctagaaca atgtctggat taatagaaat
     1081 aaatgtttcg tagtttcccg tctgttttcc aaaccagccg cctacttcag tcagattttt
     1141 gtatttaggg ttcatgtatt ggccagttgg cttgaagttc caaccggcta acttttctgg
     1201 ggccagcata taaactaagt tggttgttgg tggagaagtt gcaactactc tggtgatctg
     1261 gtccggtaca gtaacatttc taccaaccat gtctgttatt tgtgttgttc caccagttat
     1321 gggcgtataa tatgttacgt aggcacctaa agtaccaaca ataacaataa ccacgattat
     1381 ggcgaatata gtcttatttt tattatccaa cagtatcacc ccgattttag attattaaac
     1441 tgtttttttc caccagatta aaacccagtc tgctctacct tgggagtagg ttaaatttcc
     1501 ttcatgggtt ttaatcatat ttctttccaa atattctctt aaaacagatt cttcgaattt
     1561 atttaaatct ccaattctcc atttgagcct ttcaagagct tcatccacag ttgagtaatc
     1621 gcgagtgtta gattccaaca tctccacatt ggcatagaca cccatatcat ggagaatatt
     1681 gtaaacgtag atgtagtctg gatggctgta ggattctctt cccagaagat gggccatatc
     1741 cttctcaaat ttccggctat cagcccccca aatggttatg taaacgtatt tttgggctat
     1801 ttggttgatt tttttcagtt ctttctcgat attggaaaca ccattaagtg aacgggaagc
     1861 cacaattacg tcatgtttcc caatttcagc agtagatata tcttctaaac gcttattaac
     1921 aatattaata ttgttgattt ttgatttttc agcttttaca gttaatatat ccagcatttt
     1981 ttgggaaatg tccattgcag ttactttcct agcttttttg gccaatggga tggtgattac
     2041 cccattacca catccgatat ccaaaatgct atcactcggt tctatcttga ttctgcttag
     2101 aaccttttca ggatagtcat ctttttccat ccatttattg aatttaggcg caatattatc
     2161 ccaagactct gaattgtttt tatcaggcaa atttttcaat gattgattcc ataaatcatt
     2221 ccaatctact ttataatcta tttactcact ttagtattga tttgtatgca tatctcctcc
     2281 cgtatgatat tctgtaatca aatatccgat atgactatat taacatttcg gtttgatttt
     2341 ttataactta gccataacac gattaatcgc tttaatcatg aaaattagaa cattttcatt
     2401 aataatgtta ttatttattt tattaaaaat taatcgagtt ataatatttt gatattcata
     2461 ctaactgtta ggaaaataaa ataattaagg agagcttgaa tttgtattgg attctgcaga
     2521 ctaatttgct ctgccaaggt ttggaaagga tatttccatg ctcatcctct ttatgccgcg
     2581 ataaagcagt ttagagggca tacctaatac acacatcgca aaaaacacag gactgctctt
     2641 taatagatga tttattatta acgatgctaa ctacattcta tgtacaaaca tccatacaag
     2701 cataaatgaa tcactcgcat tagccagtgt cctgcaaaat ttctctttca atgactttta
     2761 taacttcagt tgcatctatg gggcagtttt cctgctaatc ctcttcatca tcccctattg
     2821 cgccattttc acatattttt tatgtatagg ccttccctag gtggtcttga tttttccaca
     2881 tttatccatg tcaataacca caggtttagt ggacaacctt tgaactgata tcgttcttat
     2941 gtgatatgga gattaaacat gtagaaaatt agaaaacctc tttcatcata aaatctgaga
     3001 acaaaaaaat cagtattaat ggtctaaaaa ttcccaaacg ctgcttatta tcatttaata
     3061 ataaaaaaaa ggaaaataaa aagaatagta aacctattct tttccaacca tgacttcggt
     3121 agattttatg atggctacta ctgcatctcc tactttaata tccagatctt ggacagaatc
     3181 tttggttatt atggcagtga ttatgtcagg agcttctatt tttattttaa cattggccat
     3241 cactgctcca gcttctactc cttccacttt tcctttgagc atattccgtg cactcatttt
     3301 cataacatca ccccatcatc gatatgcata tatattattt aatttgcaat acaaatactt
     3361 gatgcaaaaa tttacaattc gaagtacctt taacggtgat agaccatgaa ttcagtaaaa
     3421 tacgaaccaa ttggaactat acattccccc tacaaagatc tccatggaat gcccatccaa
     3481 cccattggtg cccagggagt taaaggaaaa attgaactta aaaacgagta caaagaaggt
     3541 ttaaaagatc tccaaggatt ttctcacatc atcctactgt accatctcca cctgtgcaat
     3601 ggtcattctc tgaa
//
LOCUS       DAOQVW010000114         3611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29287_length_3611_cov_3.179696, whole genome shotgun sequence.
ACCESSION   DAOQVW010000114 DAOQVW010000000
VERSION     DAOQVW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29287_length_3611_cov_3.179696"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1207
                     /locus_tag="PKK85_03450"
     CDS             332..1207
                     /locus_tag="PKK85_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25104.1"
                     /translation="MFSLDLLLALIPLTLLLGLVAADMDNILFQAEDAIFRGSTERIA
                     ADAVSTLLETSGDPTSWEQTGNPTVPGLAQYDAFKGIPIEGTISSQKLASLTLDDLQT
                     MVGSEYGVYMTVSSITSTGSNGANIKTLGTYNSNAQDIVRVERVARYALLQAVSKSEG
                     EIRGSGVSREYYDPPHKFATSYYYNQTYDYWVVFDNHGYEPSNVNLTINTNTITFTDL
                     TDPVQLDPSNLKLDESNPSEFFNNTVKLIAIAPPGAWMNFYIVQVPKGTSSNVITLSN
                     IKPEPCRLEFYIWTT"
     gene            1223..3412
                     /locus_tag="PKK85_03455"
     CDS             1223..3412
                     /locus_tag="PKK85_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25105.1"
                     /translation="MDEKGFIFTTDSILALAVVIVFTASVMTYFYAPIYMGSDHQHLE
                     ALADSALNVMEQDGTITNAAAESYKGNYDGAKYILNSRLRMLIPSDIAYNMTLTSTDT
                     ISVENDRGMLYSRDTATKVRVLSLPREGWMGRAWYKIEKFEFEDNEQNVTTTLWNFHN
                     WLTNFSPWNNGLGTVGYWGASSSTPYRPVSISFSFPSGSTILGAKYLSGSGYAGDPPQ
                     YNPGNAFSTNLTLNGVNVYTANQSQYIFLNTRPGSSRQRMYNYQGIFNVSQLNTGVNN
                     FYLRFFNTTTNVNMPWFGIIGNYTTSFPVPKGIVSQTFRFNDAAGMAVPTAQNLGGGG
                     NEYGRIYDLYTGGVTSFTTRRSIPWSSFRLQDASNYGGVNYQNGEPFVMTGVSGEGIP
                     SAVTSTKCAVSVTQDVYVPAGNVIFDAFTVINPYGGVDGAMVEVYNGTHWRTVFRSFN
                     SNEGTFSDRTDGYGNIPGIVSIPTTYLSTGRNNKIRVTIWDDVPSSDYDLVGLVDCYT
                     TVTYSALNIGWVNSYYDNKQVNSNIQTQTKSFDIDVDASNVFLFVGTGLDSKHLKVAY
                     PPAMGGHVLYDSDTIPYYLDIAALDAAGPDLVGNDGPHRITTPNSTASKYWLKQGRQY
                     NLTVTVTAPSNAWESGDGATSPPRYANAEIFTGTRISVLYPEVLRNVWTLAYNDTATS
                     AMNKAKEDLIRNLNTSDPHIINNIKTEALFTGDFPNQIPVRLSLWRQ"
     gene            3423..>3611
                     /locus_tag="PKK85_03460"
     CDS             3423..>3611
                     /locus_tag="PKK85_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25106.1"
                     /translation="MDEKGFSFTPMAFLIMIPILLIAISFTGIVNEINAISQIAIGGD
                     VTYTAANNIIISIEKGGKD"
BASE COUNT         1093 a          783 c          750 g          985 t
ORIGIN      
        1 cagcaaaagt ggcaaccaga atcaccacca caatgatgaa acccatcatc aggatgtatt
       61 ccgccgaaat ttgccctcta tcattcatac ggatcaattt ttttagctat atctaatttt
      121 attataccag aacctcttta ttataattaa ggatttacca atcccctgag tattaaatta
      181 attggtagtg gataataaaa acattgtctt catcgacaca tatactcctt catgagcaaa
      241 catatatatg cttctttact tatataaaat atagtataaa atatcagagg tcagtacatt
      301 gggactactg gaacgagact cgaggggata catgttttct ttagacttat tgttagctct
      361 gatacccctc acattgttac tgggcctcgt ggctgcagat atggataaca tactattcca
      421 ggcagaagat gctattttcc gcggatccac agagcgaata gcagcggacg cggtttcaac
      481 tcttttagaa acatctggtg atccgacttc ttgggaacaa actggaaatc caactgttcc
      541 tggcctggct caatacgatg cctttaaagg gataccgata gagggaacca tatcttcaca
      601 gaaattagcg tccctgacat tagacgatct ccagactatg gtgggaagtg aatacggagt
      661 ttatatgacg gtgtctagca taaccagcac agggagtaat ggtgcgaata taaagaccct
      721 gggaacttac aattcaaatg cccaggacat cgtcagagtt gagagagtag ctcgatacgc
      781 gctattacaa gcagtttcca aaagtgaagg tgaaataagg ggttcagggg tctccagaga
      841 atattacgat ccaccccaca aatttgccac cagctactac tacaatcaaa cctacgatta
      901 ctgggtggtg tttgataatc atggatatga accgtctaat gttaatttaa ccataaacac
      961 caacaccatt actttcacgg atttaacaga tcccgtacag ttagatccta gtaatctaaa
     1021 attagacgag agtaatccct ctgaattttt taacaacaca gttaagttaa tagctattgc
     1081 tcctccggga gcttggatga atttctacat tgtacaagtt ccaaaaggca cttcatcaaa
     1141 tgtaatcaca ctaagtaata ttaaaccaga accatgcaga ttagagtttt atatatggac
     1201 aacttaaggg agcagtgtta aaatggatga aaaaggattt atattcacca cggattcaat
     1261 cctggccctt gccgtagtca tagtttttac agcatccgtt atgacctact tctacgctcc
     1321 aatatacatg ggctctgacc accaacacct ggaggccctg gccgacagtg cactgaatgt
     1381 gatggaacag gatggcacca taaccaacgc cgcagcagag tcctataagg gaaactacga
     1441 tggagctaaa tatatattaa acagccgtct tagaatgttg atacccagtg atattgccta
     1501 caatatgact ttaacctcca ctgatacaat ttctgttgaa aatgatcggg ggatgcttta
     1561 ttcacgagac accgccacca aggtccgggt actatcgtta ccaagggaag gatggatggg
     1621 acgtgcctgg tacaaaatag agaaattcga atttgaagat aatgaacaaa acgtgaccac
     1681 caccctgtgg aacttccaca actggcttac caacttctca ccctggaata atggattggg
     1741 tactgttggt tactggggtg caagtagtag tacaccttat aggccagtca gcatatcctt
     1801 ttcattcccc agtggttcta ccatattagg tgccaaatac ctttctggtt ctggttatgc
     1861 aggtgaccca ccacaatata atccaggtaa tgctttttca actaacctaa ccttaaatgg
     1921 ggtgaatgtt tacactgcaa atcagagcca gtacatattt ttgaataccc ggccgggatc
     1981 atcacgccag cgtatgtata actaccaggg catttttaac gttagtcaac ttaacactgg
     2041 agttaataat ttctatttaa gatttttcaa cacaaccacc aatgttaaca tgccctggtt
     2101 cggaattata gggaattata ccaccagctt ccctgtgccc aaggggattg tatcccaaac
     2161 cttccgtttc aatgatgccg ctggaatggc agttccaaca gctcagaact tgggtggagg
     2221 tggaaatgaa tacgggagaa tttatgatct ttataccggg ggagttacga gttttacaac
     2281 cagaagaagt attccctgga gttcatttag actacaggat gcttcaaatt atgggggggt
     2341 gaattaccaa aatggtgaac ccttcgtaat gaccggtgtt tctggggaag gtattcccag
     2401 cgcagtaact tcaacaaagt gtgcagtcag tgttacccaa gatgtgtatg ttcctgctgg
     2461 aaacgtcata ttcgatgcct ttacagtaat taacccttac ggtggggttg acggggccat
     2521 ggttgaagtt tacaatggga cgcattggag aactgttttc cgttcattta actcaaatga
     2581 aggaacgttc agcgatagaa ctgatggtta tggtaacatt ccgggaattg tttccattcc
     2641 caccacctac ttatctactg ggcggaacaa taagatccgg gtgactatct gggacgatgt
     2701 tcccagcagt gactacgact tggtaggatt ggtggactgt tacaccacgg ttacctacag
     2761 tgcactgaac attggttggg tcaacagcta ctatgataat aaacaagtta acagcaacat
     2821 ccaaactcag accaagtcct ttgatataga tgtggatgca tctaatgttt tcctctttgt
     2881 tggcactggc cttgattcca aacatttaaa ggtggcatat ccacctgcca tgggtggaca
     2941 tgtcctttac gatagcgata ccatccctta ttatttagat atagcagctc tggatgctgc
     3001 agggcctgat ctagttggca atgatgggcc tcacagaata accaccccta actccacagc
     3061 gtcgaaatat tggctcaaac aggggcgtca atacaatctt acagtgacgg taactgctcc
     3121 ttccaatgca tgggaatcgg gtgatggagc taccagtccc cccagatatg ctaatgccga
     3181 gattttcact ggaaccagga tttctgttct ctacccagaa gttttaagga acgtgtggac
     3241 cctggcatat aacgacacag caaccagtgc catgaacaag gccaaagaag atcttattcg
     3301 gaatttgaac actagtgatc cacacattat aaacaacatt aaaacagaag ctctcttcac
     3361 aggagacttc cctaaccaga tcccagtcag gctaagccta tggaggcaat gaggtgatat
     3421 aaatggacga aaaaggattt tcattcactc caatggcctt tttgataatg atacccattt
     3481 tactcatagc aatttctttc actggcattg taaatgagat taacgccata tctcaaatag
     3541 ctattggagg ggatgtcacc tacaccgctg ccaacaacat tataatttcc atagaaaaag
     3601 gtggtaagga t
//
LOCUS       DAOQVW010000115         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29314_length_3609_cov_3.255768, whole genome shotgun sequence.
ACCESSION   DAOQVW010000115 DAOQVW010000000
VERSION     DAOQVW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29314_length_3609_cov_3.255768"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PKK85_03465"
     CDS             complement(<1..1239)
                     /locus_tag="PKK85_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNS25107.1"
                     /translation="MDRFKLLSDYRPLGDQPQAIRSLTEGIKKGYRDQTLLGVTGSGK
                     TFTMANVIQDVQKPTLIISHNKTLAAQLYEEFREFFPNNAVEYFVSYYDYYQPEAYVP
                     RTDTYIDKESSINEEIDMLRHSTTQSLLSRDDVIVVASVSCIYGIGAPEDYGDMIMRL
                     EIGDQLDRQEILAKLVEMQYERNDVDFSRGKFRVRGDTIEVFPAQGKTPIRIELFGDE
                     VDGLSFIDHLSGRGTRNLEKVTIFPAKHFVTSQDKLESAISKIRVELEERLEELKLQN
                     KLVEAQRLEQRTQFDLEMLQEMGYCNGIENYSMHMSGRQWGETPYSLLRYFPDDYLTI
                     IDESHVTVPQIRGMYAGDRARKEVLVDYGFRLPSALENRPLNFDEFMGLQNQVVYVSA
                     TPAAFELERSQQVVEQIIRPT"
     gene            1422..3227
                     /gene="uvrC"
                     /locus_tag="PKK85_03470"
     CDS             1422..3227
                     /gene="uvrC"
                     /locus_tag="PKK85_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNS25108.1"
                     /translation="MTTSSAPDENLPDGPGVYLMYDPKDNILYVGKAKSLKKRVKSYF
                     REEIEDPKTRALMRQFHHLEYMVTDSEKEALILESNLIKKHLPRYNIRLKDDKRYPYL
                     KITREDFPRLLVTRIYEDDGADYYGPYTDVKAVRNIIKFLKPVFPLRDCKRMDGPCLN
                     YQIKLCPAPCSGQVTKEEYQKQIKQVKLFLEGRYDDIKAIMRDEMLDAASEKEFEKAA
                     ALRDQLNSLDDILEKQKMELNRNLDQDVIAASADTELAVVVVFSLRAGKIMSKEDFIM
                     EGLSQGLTEDDNSIPESVYSAFLKQYYSGPRPVPGEILIPQLVKDEKLILEWLREKKG
                     DMVALKIPETNLERSMVRMVAKNAQIIKDNQKEFRGALLDLKGYLHIPHLPRYIEAFD
                     ISNLSGQMAVGSMVVFEDGKPKKNAYRRFKIKTPGPDDYGMMRELLERRYHKHSFYDE
                     SAGEQVPDLILVDGGRGQLNVALDVLHSTGIQTGVIGLAKEFEHVFVPEVSSPIILPP
                     KSPAMHLLQRIRDEAHRFAISYHRKLRSQEMEHSILDDIPGVGPKRKQKLLKHFGSLE
                     QVKSAGVDELAQVEGISSNLALVIQEFLQKNNSES"
     gene            complement(3289..>3609)
                     /locus_tag="PKK85_03475"
     CDS             complement(3289..>3609)
                     /locus_tag="PKK85_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HNS25109.1"
                     /translation="KEEEAPAKTGKGPCIILTDASGRGLITHPQVKELLIEVAEEENI
                     PYQLEVSEGGTTDASAIHLTREGIPTGVISPASRYIHTPVSVVNIKDVEMVVELILAV
                     LRRV"
BASE COUNT          962 a          875 c          871 g          901 t
ORIGIN      
        1 tgtggggcgg atgatctgct ccaccacctg ctggctgcgt tccagttcaa aggctgcggg
       61 ggtggcggat acatagacca cttggttctg gaggcccatg aactcatcga agttcagtgg
      121 ccggttctct agggccgagg gtaatcggaa tccataatca accagaacct ccttacgggc
      181 ccggtcccca gcgtacatac cccgtatctg ggggacagtt acatgtgatt catcgattat
      241 ggtcaggtaa tcatccggga agtagcgcag taaggagtag ggtgtttcac cccactgtcg
      301 tccggacatg tgcatgctgt agttttcgat accattacag tatcccattt cctgcagcat
      361 ctccaggtca aactgggtgc gctgctccag gcgctgagcc tccaccaact tgttctggag
      421 tttcaactcc tccagtcgct cctccagttc aaccctgatc ttactgatgg cactttccag
      481 cttatcctgg ctggttacga agtgcttagc cgggaagata gtcactttct ccagattccg
      541 ggttccccta ccactcaggt gatctatgaa tgataacccg tctacttcat ccccgaagag
      601 ttcaatgcgg atgggtgttt tgccctgggc cgggaacact tcaatggtgt cgccccgcac
      661 ccggaacttg cccctggaaa agtccacgtc gttgcgttcg tactgcattt ccaccagttt
      721 ggccaggatt tcctgccggt ctaattggtc accaatttcc aggcgcatta tcatatcccc
      781 gtagtcttca ggtgctccaa taccatagat acaggacaca ctggccacca ctatcacgtc
      841 atcccgggat aggagtgact gggtggtgga gtggcgcagc atatcgattt cttcgttgat
      901 agaggactcc ttatcgatgt aggtgtcggt gcggggcacg taggcctcgg gctggtagta
      961 gtcatagtaa ctgacgaagt actccacggc gttgttgggg aaaaactccc ggaactcctc
     1021 gtagagttgg gcagccaggg ttttattgtg tgatatgatc agggtaggct tctggacatc
     1081 ctggatgaca ttggccatgg tgaaggtttt accggagcca gtaactccta agagggtctg
     1141 gtcacggtaa cccttcttaa taccttctgt tagggacctt atggcctggg gttgatcccc
     1201 taagggcctg taatctgata aaagtttaaa tctatccata acttgattct caccactgga
     1261 aattaaagtt aaaaataaaa tcttatctaa acgttataaa aaatagatac aatatcttac
     1321 cctattctaa tatataaaga tcactaccaa tacattatac cccctcatga atcttagcac
     1381 cccccctcta agtataaaac tagaattatg gtgaagctac attgaccact tcttcagccc
     1441 ctgatgaaaa cctgcccgat ggacccggtg tttatctaat gtatgatccg aaggataaca
     1501 tactctacgt gggcaaggct aagtccctga aaaagagagt taagtcttac ttcagggagg
     1561 aaattgagga ccccaagacc cgggccctga tgaggcagtt ccaccacctg gaatatatgg
     1621 tgacggacag tgaaaaggaa gccctgattc tggaatcaaa ccttataaag aagcatcttc
     1681 cccgatacaa catccgcctc aaggatgata aacgctaccc ctacttgaag atcaccaggg
     1741 aagactttcc aaggctcttg gtgacccgca tctatgagga tgatggagct gattactacg
     1801 gcccctacac tgatgtgaaa gctgtccgta atatcatcaa attcctgaag ccagtgttcc
     1861 ctctcaggga ctgtaaaagg atggatgggc cctgtctcaa ctaccagatc aaactgtgcc
     1921 ccgccccctg cagtggacag gtgactaaag aagagtacca gaaacagatc aaacaagtta
     1981 aacttttctt agaaggccgc tatgatgata taaaagccat tatgagggat gaaatgctag
     2041 acgctgcttc agagaaggaa ttcgaaaagg ccgctgccct gcgggatcag ctgaactccc
     2101 tggatgatat tctggaaaaa caaaagatgg aacttaacag aaacctggat caggatgtta
     2161 tcgcggcatc tgcagataca gaactggctg tggtggtggt gttctccctc cgtgccggta
     2221 agataatgtc caaggaggac ttcataatgg agggcctgtc ccagggatta actgaggatg
     2281 ataacagcat cccagaaagc gtttattccg cttttctgaa acagtactac tccggaccca
     2341 gacctgtccc aggggaaata ctcatccccc agctggtgaa agacgagaaa ctcattctgg
     2401 aatggctccg ggaaaagaaa ggtgatatgg tcgccctgaa aatacctgaa accaatctgg
     2461 agcgcagtat ggtgcggatg gtggccaaga atgcccagat tattaaggat aatcagaaag
     2521 aatttagagg cgcactactg gatctcaaag gctacctgca cattccccac ctcccccgat
     2581 acatagaagc atttgacata tcaaaccttt ctgggcagat ggctgtgggc tcaatggtgg
     2641 tatttgagga tggaaaacca aaaaagaatg catatcgtcg ttttaaaatt aagacgcctg
     2701 gtccggatga ctatggaatg atgcgggaac tactggaacg gcgctaccat aaacacagct
     2761 tctatgatga gtcagcaggg gagcaagtac cggatctgat acttgtggat gggggccgtg
     2821 ggcagctaaa cgtggccctg gacgttcttc attctactgg gatccagact ggagtcatag
     2881 gattggcaaa ggaatttgaa catgtattcg ttcctgaagt ctcctcaccc atcatactac
     2941 ctcctaaatc accagcgatg cacctgttgc aaaggatccg ggatgaagcc caccgttttg
     3001 ccatcagcta ccacagaaaa ctccgttccc aggaaatgga acattccata ctagatgaca
     3061 taccaggagt gggccctaaa aggaagcaga aactattaaa acatttcggc agtttagaac
     3121 aggtgaaaag tgcaggtgtt gatgaactgg cccaggtgga gggtataagt agcaacctcg
     3181 ccctggtgat ccaggaattc ctgcaaaaga acaattcaga atcttaaaaa aaacttgttt
     3241 tgtagaaaaa tatgggatta tcccggatat aattccttaa agcttgtttt aaactcttct
     3301 cagtacggcc aggatcagtt ccaccaccat ctccacatct ttgatgttaa ccacactcac
     3361 tggtgtgtgt atgtaacggg aagcaggtga tataactcca gtgggtattc cctcccgggt
     3421 gaggtgtatg gcactggcat cagttgtacc tccttcactg acctccagct ggtaggggat
     3481 attttcctct tcggctactt caatcaggag ttctttaacc tgggggtgag ttatcaggcc
     3541 ccttccactg gcatcagtga gtattatgca gggtccttta ccagttttag caggtgcttc
     3601 ctcctcctt
//
LOCUS       DAOQVW010000116         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29506_length_3591_cov_3.102658, whole genome shotgun sequence.
ACCESSION   DAOQVW010000116 DAOQVW010000000
VERSION     DAOQVW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29506_length_3591_cov_3.102658"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /gene="pfdA"
                     /locus_tag="PKK85_03480"
     CDS             complement(<1..200)
                     /gene="pfdA"
                     /locus_tag="PKK85_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HNS25110.1"
                     /translation="MDDRQRLEELINELNMYKAQAELLNQQIETLKVSISELVMALET
                     LDELKGKKGVETFVPIGAGSFL"
     gene            complement(208..438)
                     /gene="rpl18a"
                     /locus_tag="PKK85_03485"
     CDS             complement(208..438)
                     /gene="rpl18a"
                     /locus_tag="PKK85_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HNS25111.1"
                     /translation="MKTKIFRIQGKFMMGDRLKPFTRELRATSEADIREKIYSEFGSK
                     HHITRNQIKIQDIQEISADDVLDPLIKALIQE"
     gene            complement(435..1109)
                     /locus_tag="PKK85_03490"
     CDS             complement(435..1109)
                     /locus_tag="PKK85_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HNS25112.1"
                     /translation="MIRRFNLRGNPNLGVAISATDQAALVPPNLEDKDLQLIEEALQV
                     PLIKTHISGSNLAGALTVGNSQGFVVSKYAFDREVDAIRQTGLAVERIPDRLTAVGNI
                     VLVNDHGAIINPLLSDKSAELISSTLDVDVERGKIANFKITGSVALATNKGVLVHPSA
                     TPDELKLLESTLKVPADVGTVNRGTRLVGSGSVANSHGVLVGLDTTGPEMARIEESLG
                     FLEVYL"
     gene            complement(1127..1369)
                     /locus_tag="PKK85_03495"
     CDS             complement(1127..1369)
                     /locus_tag="PKK85_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HNS25113.1"
                     /translation="MERVYTIPLRDVKKVPRTKRSPRATRYVRDFIQRHMKSEDVKID
                     ASVNEKIWERGIQKIPPRIKVKATKEDDGSVLVTLA"
     gene            complement(1381..1536)
                     /locus_tag="PKK85_03500"
     CDS             complement(1381..1536)
                     /locus_tag="PKK85_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HNS25114.1"
                     /translation="MSRNKPLAKKLRLAKAYKQNRRVPLWAMLKTSRKVRSHPKMRHW
                     RRSKIKV"
     gene            complement(1523..2125)
                     /locus_tag="PKK85_03505"
     CDS             complement(1523..2125)
                     /locus_tag="PKK85_03505"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNS25115.1"
                     /translation="MKAAVLYSGGKDSSLMAVILHRLGYQVELLTANFGVYPSYKPAK
                     EAAKNLGFSHRVLRADKTLLENAVSRILEDGYPNQGIHYLHRQILEVAASGYGVVADG
                     VRRDDRVPRLSHREVASFQDRHGVEYINLSGFGHRTVNNLGKNLFKLKKEPTNPHNNS
                     DYEIEIRHLIREKKGIKIAESLFPPHTQSRVIGWRENESK"
     gene            complement(2122..2469)
                     /locus_tag="PKK85_03510"
     CDS             complement(2122..2469)
                     /locus_tag="PKK85_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNS25116.1"
                     /translation="MSDIEEIRRRRMEQLQQQQAAQQMSQQEIEAQERMRQEMEAQKR
                     QIMMQILTPEARSRLANLRLTRPDYVNQIELQLIQLAQAGRIASKITDEQLKELLRKL
                     GASQKKEIKIVRK"
     gene            complement(2485..2922)
                     /locus_tag="PKK85_03515"
     CDS             complement(2485..2922)
                     /locus_tag="PKK85_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HNS25117.1"
                     /translation="MTTVYDVPADSLISEVAKELSENKKITPPEWAAFVKTGVHKERR
                     PDDADWWYVRCASLLRRVYMDGPVGINSLRTYYGGSKDRGTSPEKFRRGSGSIVRGAL
                     HQLEDAGLVEKVDEGRRVTSEGRSFLDKASHQIKKDIPQAAKY"
     gene            complement(2933..3169)
                     /locus_tag="PKK85_03520"
     CDS             complement(2933..3169)
                     /locus_tag="PKK85_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HNS25118.1"
                     /translation="MNRSLSTITINIGKSGVNPGVLDEIKRHLKEREVVKLRFSKGIS
                     PEKQNYITQITEKSNAKLVDFRGNVAVIFKKRGN"
     gene            complement(3162..3533)
                     /locus_tag="PKK85_03525"
     CDS             complement(3162..3533)
                     /locus_tag="PKK85_03525"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 4"
                     /protein_id="HNS25119.1"
                     /translation="MRRGRRPKSMIKIAYERLEILFKMAEEEFSLHPLRSHRYVEMAR
                     NIAQKYNLKMPSYWRCRYCKGCKRFLKPGLNSKVRLNQQAVTVKCLECGEVSTMPYVK
                     EQKSKRRKKIEPRTFQEGTHE"
BASE COUNT          784 a          885 c          755 g         1167 t
ORIGIN      
        1 atcaggaatg aacctgcacc aatgggtacg aaggtttcaa ctcctttttt accctttaac
       61 tcgtccaatg tttccagggc cattaccagt tcagatatac taacttttag ggtttcgatt
      121 tgctgattta aaagttcggc ctgggctttg tacatgttga gctcgtttat aagctcttcc
      181 agtcgctgac ggtcatccat ggaatgatca ctcctgaatt agggccttga tcagtgggtc
      241 caggacatcg tctgctgata tttcctggat atcctgaatt tttatctgat tccttgttat
      301 gtggtgtttg cttccgaatt cagagtagat cttctccctg atatcagctt cgctggtggc
      361 tctcaattcc cgggtgaagg gtttgagtct gtcccccatc ataaatttac cttgtattct
      421 aaatatcttt gttttcataa atatacctcg agaaaaccca atgattcttc gattcttgcc
      481 atttccggac cagtcgtatc cagacccacc aacacgccgt ggctgttggc cactgacccg
      541 gaccccacca ggcgagtacc ccggttaacg gtacccacat ctgcaggaac cttcaaagtc
      601 gattccagaa gttttaattc atctggagtt gcagatggat gtactaaaac tcctttattg
      661 gtagctagtg ctactgatcc agtgatctta aaatttgcta tttttcccct ttctacatcc
      721 acatccaggg tgctggatat taactctgca gatttatcag agaggagggg attaattatc
      781 gccccgtggt catttaccaa gacgatgttg cctactgcag tgagcctgtc aggtattctc
      841 tccactgcca gcccagtttg acggatagca tctacttccc ggtcaaaggc atatttggat
      901 accacgaatc cctgggagtt tccaacagtc aaagctccgg ctagattgct gccgctgatg
      961 tgggtcttga ttagtggtac ctggagtgct tcctcaatta attggaggtc cttgtcttcc
     1021 aggttagggg gaaccagtgc cgcctgatca gtggcagaaa tggccactcc caggttgggg
     1081 ttgcccctca agttaaatct cctgatcata ggttcaccta ttacattcag gccagggtga
     1141 ccaaaaccga accatcatct tctttggtgg cctttacctt aatcctgggg ggtattttct
     1201 ggattcccct ttcccatatc ttctcgttga ctgaggcgtc aattttaacg tcctcggact
     1261 tcatgtgtct ctggatgaaa tcccttacat atcgggttgc ccggggagat ctcttagtcc
     1321 ttgggacttt tttaacatct ctcaggggaa tggtgtaaac tctttccatg ttcaatctcc
     1381 ttatactttg atcttacttc ttctccagtg tctcattttg gggtgagacc tcactttacg
     1441 ggaggttttt agcatggccc acagtggcac ccttcggttt tgtttatatg cctttgccag
     1501 tctaagtttt tttgctagag gtttatttcg actcattttc tctccatcct attactcttg
     1561 attgtgtgtg ggggggaaaa aggctttctg caatttttat tccttttttc tcccggataa
     1621 ggtgtctaat ctctatctcg tagtctgagt tgttgtgggg attggttggt tcttttttaa
     1681 gcttgaaaag attttttccc aagttgttta cagtccgatg cccaaaaccg ctgaggttga
     1741 tgtactccac accatggcgg tcctggaagc tggctacctc ccggtggctt agtctgggaa
     1801 cccggtcatc gcggcgtaca ccatcggcca ccaccccata cccagatgca gccacttcta
     1861 aaatctgccg gtgcaggtaa tgaataccct ggttgggata accatcctcc aatatcctgg
     1921 aaacagcgtt ctccagtaaa gttttatcag cacgtaagac ccggtggctg aagcccagat
     1981 tttttgcagc ttctttggct ggtttatagg aaggataaac accaaaattt gcggttaaaa
     2041 gctctacctg atatcctaat ctatggagga taacagccat gagggaactg tctttacccc
     2101 cactgtagag taccgctgct ttcattttct tacaatcttt atttctttct tctggctagc
     2161 ccctaatttt cgtaaaagct cctttaactg ttcatcagtt attttagaag ctattcttcc
     2221 tgcctgagcc agttgaatga gctgaagttc gatttgattc acataatccg gccgggtcag
     2281 tcggagatta gccagtctac tgcgggcctc aggggtgagg atctgcatca ttatttgcct
     2341 tttttgtgcc tccatctcct gacgcatgcg ctcctgggcc tcaatttctt gttgagacat
     2401 ttgttgagct gcctgctgct gttgtaactg ttccattctt cgacggcgta tctcttcgat
     2461 atcgctcatt taaaaaatcc ccatttaata cttggctgct tgtggtatgt cttttttaat
     2521 ctggtgggat gctttgtcca ggaaggaccg tccctcagag gtaactcttc ttccttcatc
     2581 caccttctcc accagtccag catcctccag ttggtgcagg gcccccctga ctatggaacc
     2641 gctgcccctt ctgaattttt cagggctggt accacggtcc ttgctaccac catagtaagt
     2701 tcgcagactg ttgataccca caggaccatc catgtaaacc cttcttaaaa gggaagcgca
     2761 acgtacgtac caccagtcag catcatcagg ccttctttcc ttgtgaacac ctgtttttac
     2821 aaaggcagcc cattctggtg gggtgatctt tttattttca cttaattcct tggcgacttc
     2881 gctgatgagc gagtcggcag gcacatcata aactgtagtc atatttatcc tcctaattac
     2941 ctcttttttt gaatattaca gcaacgttac ctctaaaatc aaccagttta gcattggatt
     3001 tttcggtgat ctgagtaata tagttttgtt tctcgggtga tatacctttc gaaaagcgga
     3061 gcttaaccac ttctcgttcc ttcagatgtc tcttgatctc atccagcact ccagggttga
     3121 ctccggattt gcctatgttg atggtaatgg tcgaaagtga tctattcatg agttccttct
     3181 tggaaggtgc ggggttcaat ttttttcctc ctcttggact tctgctcctt tacatagggc
     3241 atggtactga cttctccaca ttccaggcac tttacagtca ctgcttgttg gttaagcctg
     3301 actttagagt ttaatcctgg ctttaaaaac cttttacacc ctttacagta cctgcacctc
     3361 cagtaggaag gcatcttcag gttatacttc tgggcaatgt tccgggccat ctccacgtag
     3421 cggtgcgacc gtaatgggtg gagggaaaat tcttcttccg ccattttgaa gaggatttca
     3481 agcctttcat aggctatttt tatcatcgac ttcggtcttc ttccccttcg caaattttaa
     3541 cctctcaaga aacatcgaag atgctaagaa tcctaagttt ctcaaaagac a
//
LOCUS       DAOQVW010000117         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29603_length_3584_cov_2.808728, whole genome shotgun sequence.
ACCESSION   DAOQVW010000117 DAOQVW010000000
VERSION     DAOQVW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29603_length_3584_cov_2.808728"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..594
                     /locus_tag="PKK85_03530"
     CDS             163..594
                     /locus_tag="PKK85_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25120.1"
                     /translation="MIVFPHGSPANPKKGPEETAINDYICNLYVDAFGVPVIYINSFG
                     KLEHMPGIMGLMMKLAGFRMNGKTEIYALGGEGILHGIKGVKGHAINLSEKKRKKDIE
                     FFGRDLIKGNFLFRHFILAPDILMGIRRYNKGLKKCTSYKA"
     gene            1135..1728
                     /locus_tag="PKK85_03535"
     CDS             1135..1728
                     /locus_tag="PKK85_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNS25121.1"
                     /translation="MLGILLSELSEENVEWFYHINWGLENPILDFIMPIISTLGSLYG
                     WIIFSLVIYLFGARLGRRTALLALFALFLSNILVLLLKYVVGEPRPFLALPNVDLLMH
                     AEGNSFPSGHTASSFAAATVIGLKYYLESDGRLRLLIYPCMVFAFLVGLSRIYVGVHY
                     PYDVIIGALIGVVCAPVVLKYQNRILESRVGCLLGGN"
     gene            complement(2119..2700)
                     /locus_tag="PKK85_03540"
     CDS             complement(2119..2700)
                     /locus_tag="PKK85_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNS25122.1"
                     /translation="MMEKKLKEKKEEAEEKAVEIKKATLEKKKKAEEKGAELIEAAAQ
                     IKEEKEEEARRSGKQAEKVINDFLSSLRSRHEDFSKAWSDYITSEKPLADVIETESEI
                     IIKTDLPGIKKGDVDVTITEDSVEIMAHFKEEYSEEEVDYVMRERSYGETKRFIKLPG
                     KVKIKEATAKLENSILTIKLPKLEKSKFKVDID"
     gene            complement(2859..3110)
                     /locus_tag="PKK85_03545"
     CDS             complement(2859..3110)
                     /locus_tag="PKK85_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNS25123.1"
                     /translation="MMRNEFAVASLLLGIISFVQFLGIEKAALAIIFGVLALNEIRGS
                     EMSGKYLAILGIILGFIYVVLVITILPYLYQYATQIMAG"
     gene            3400..>3584
                     /locus_tag="PKK85_03550"
     CDS             3400..>3584
                     /locus_tag="PKK85_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS25124.1"
                     /translation="MSLEIRTIQLKIMSGLATVNCYLLISGEDYLLIDSGHSSKQDEL
                     DGKLRQAGCQAGDLKLVL"
BASE COUNT          941 a          764 c          725 g         1154 t
ORIGIN      
        1 gtatagggca ttgttacaaa aagcgaggga gaagcagcag tttttaaaag aggctggttt
       61 gatagtatta ttaaaacacc atttggaaat gtagctgttg ccatttgcta tgatgccagg
      121 cgtaaacatt tttatgagaa tatcaaagac gaagaaattt ctttgattgt tttccctcat
      181 ggtagcccag ctaatcccaa gaaaggtccc gaggaaactg ccattaacga ttatatttgt
      241 aatttgtatg ttgatgcttt tggcgttcct gtgatctaca tcaatagctt cgggaaacta
      301 gaacacatgc caggcataat gggtttaatg atgaaattag ccgggtttag gatgaatggt
      361 aaaactgaaa tttatgctct tggtggtgag ggcattttac atggcattaa aggtgtgaaa
      421 ggccatgcta taaacctttc agagaagaaa agaaaaaagg atatagaatt tttcggccgt
      481 gacttaatta agggcaattt cctgtttcgg cacttcatac ttgcacctga tattttaatg
      541 gggataagac gatataataa aggccttaaa aagtgcactt cttataaagc ctaaagagtc
      601 aagcccatgt tttttgtgtg aatttacttt tagttagctc cacccttctg atgacctgat
      661 cttgttcctg atcggcctgt gcaagggcca atgcacacta gttcagctct attgtccatt
      721 gtttgcaacc ctgacggctt aaaaagtaat tgagaaatag ccgggcatca tcatcaaagg
      781 aactgaaaca aagattataa tttcctgatc tttatttcat ttgctgtagc catttttgct
      841 gaaagattgt tttgtgccgt tctaatgatg ataatgtccg tatggttggt gtgaattcga
      901 aaacaaagag gtacatatca tcctggcaga ggacggagat atctccaccc tgctgtaggc
      961 cttataaaac cagggaaaaa gtgttagttc tagatcctct cccttccatt aatccttctt
     1021 ggccactatt aaaccgaagc taataaggga caggaaagtt aatgccagtt agagagttat
     1081 aaagtatcac agggtaaggt gacaataggg tggattcttt acttggggaa gggtatgtta
     1141 ggaattttat tatctgaact atcagaagag aacgtagagt ggttctacca tataaactgg
     1201 ggtttagaaa atcccatact tgattttata atgcccatca tctctacctt gggaagcttg
     1261 tatggatgga taatattttc cctggtgatc tacctatttg gtgctagact cggcagaaga
     1321 acagccttac tagcattgtt tgccctattc cttagcaaca ttctagtgct tcttttaaaa
     1381 tatgtggtag gagaaccccg gcccttctta gccctgccca acgtggatct gctgatgcat
     1441 gcagagggca actccttccc ctcagggcac actgcctcct catttgcagc ggccacagtc
     1501 attggtttaa agtattattt ggaatcagat ggaagactcc gtttgctgat ctatccctgt
     1561 atggtcttcg cctttttggt ggggctatcc aggatctacg taggggttca ttacccctac
     1621 gatgtgatta ttggcgccct aatcggggta gtctgcgccc cggtagtgtt gaagtaccaa
     1681 aatagaatac tggaaagtag ggtgggctgc cttctaggtg gaaactaggg gtacatggga
     1741 ttgccaacca gtttccagat ggtccttgcc atttacattt ctgattcatc aaatgtacag
     1801 tctgggtgga acattttttc ctggatggtc ttgatggggg cgtggttcag ggcacgcccc
     1861 cacctagagg tccgtggcag catatttttt tttatggata aatctaaaga ttcaatcctc
     1921 tagccacgag gatattattt tttttcatcc acaccctgta ccttaagagt ccacatgtat
     1981 ttttgcttgg tctcccctta tccgatggct aacccgtcct gggttccctt attctaatag
     2041 aatccgaatc aatgggtgga ttccattcac cctccctatc cccattcagg ggccaggcct
     2101 cattggtcac agatttttct agtctatgtc caccttgaat tttgattttt ccagtttagg
     2161 gagtttgatg gttaaaatgg agttttccag tttagcagtg gcctctttga tcttgacttt
     2221 gcctggtaat ttgatgaacc ttttggtttc accgtaactt ctctccctca tgacgtagtc
     2281 cacttcctct tcactgtatt cctccttgaa gtgggccatg atctccacac tgtcttcagt
     2341 tatggtcaca tccacgtcgc ctttttttat accgggaagg tcagttttta tgatgatctc
     2401 agattcggtt tcaatgacat cggcgagtgg tttctctgag gtaatataat ctgaccaggc
     2461 cttgctgaag tcttcatgtc tactgcggag actgcttagg aaatcgttaa ttaccttctc
     2521 agcttgtttt ccacttctcc gggcttcttc ttctttttcc tctttgatct gtgctgctgc
     2581 ttcaataagt tctgctccct tctcttcggc cttctttttc ttttccaggg tggccttttt
     2641 aatctccact gccttttctt ctgcttcttc tttcttttct tttagctttt tttccatcat
     2701 caacaatcac ctacaatata gtaatcttag ttctagactc tatatttttt tcctataatc
     2761 catgattttc tccatgccag taagatctcc acatcttctt ggctgagaaa aaaaacaagg
     2821 cacctatcct tccattagtc tatgtcaacc ctagttaact agccagccat gatctgtgtg
     2881 gcgtactggt agaggtaggg taaaatagtg attaccagta ccacgtatat gaatcccagg
     2941 atgattccca ggatggccag gtacttccca ctcatttcag atcctcttat ttcatttaaa
     3001 gccaagacac caaatataat tgccagcgcc gctttttcta ttcctagaaa ctgtacgaag
     3061 ctgattattc ccaaaagcag actggcaact gcgaattcat ttctcatcat accactctcc
     3121 tgcccatatt tatgcctatt ccatcttatt acattttaat acagcaatta actataatat
     3181 gagaaatagt taaaatatta cttacctata tttaatttga aaaaaaaaga aaagggggtt
     3241 aagtaggtgt aaaatccact aaggatcctc cctattggga atacttcccc ttaacctagg
     3301 atcatttaga gacttaagac ctttctccaa ctaataaaaa taataaaccc ctctaaatgg
     3361 cgtgaaatta attacccttc cctaacaaat gattatacca tgtccctgga gatacggacc
     3421 atccagttga agatcatgtc tggcctggcc accgtcaact gttatctgct gataagtggg
     3481 gaggactacc tgctcattga ctctggacac tccagtaaac aagacgaatt agatggaaag
     3541 ttgagacagg ccggttgtca agctggggat ctgaagctgg tact
//
LOCUS       DAOQVW010000118         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29635_length_3580_cov_4.786950, whole genome shotgun sequence.
ACCESSION   DAOQVW010000118 DAOQVW010000000
VERSION     DAOQVW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29635_length_3580_cov_4.786950"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKK85_03555"
     CDS             <1..494
                     /locus_tag="PKK85_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25125.1"
                     /translation="YDPEFLEELESEHTIYAYDENQQFRGVNKKGRDIYIFDNGYCLT
                     AAKFCEFVPKLQRMGGGEVTDHLQSEIFMINNGLVSDWMGEKKTKLLEKALKCTTCQL
                     LHNDFVTYGILNLVLKDRYGVTMETDNFGKILSENDFIIEDVEEELQRIASQKLEKDK
                     KES"
     gene            1080..1712
                     /locus_tag="PKK85_03560"
     CDS             1080..1712
                     /locus_tag="PKK85_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25126.1"
                     /translation="MREYKTLVKCYQRKKSRQHLITLKGDHGFKGDDPVVVLPEEDFN
                     DLVTLQDKARDHDHELLLKDEEISRLKLDLQGSRDEIESKARDLMEHESRVSVYEDNI
                     KHLKEDLQALKDKNLQLEEILKASRDEIGEHRLRIRELEKLELEDYQGLYNKLRNKHD
                     HLQERFNKSLEEVNGLQRIISDLSNRGFIDYITGRLPESYKQLSEPKKAT"
     gene            1796..2722
                     /locus_tag="PKK85_03565"
     CDS             1796..2722
                     /locus_tag="PKK85_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25127.1"
                     /translation="MKKIYLKTKERREQIMGNHDLWIKDTETRFFAESRSFATPEQLF
                     YVTDDQRYLSYWPKGYKGKKSTLQSRNSLIGNFTEKWTTDLIQKVVNDKGLYAVQGAV
                     CDEIALSKRSPADVVISKKKAINLKPEDIVLIIEVKMSVVWNWQLSINPSCEELVCIG
                     NYKTHQGNPGLLRSDSMLKAIGKSINIRVSSFKASNIPIIILGNTPITDSYYSKVDHL
                     QKAGIIQGFWSVNPDPLDGAESIKETEEQGFYRFDNFEELKTSFDLLLEEELNFFSSM
                     KTKNELGQIIELSDKEETYERKAEVFLDLIRE"
     gene            2727..>3580
                     /locus_tag="PKK85_03570"
     CDS             2727..>3580
                     /locus_tag="PKK85_03570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNS25128.1"
                     /translation="MSTKRKERKGTKTSSFGSSGRANHDSSEFYGSKLYSEIEIPKKV
                     KFIENEIAPSKLDKIYCKSSEKMEEIPDNSVHLMVTSPPYNVKKEYDEDLTLREYMDL
                     LRNIFQETYNKLVTGGRVCINIANVGRKPYVPLHAHIIDIMLNIGFIMRGEIIWDKSA
                     SAGSSCAWGSWKSASNPVLRDVHEYILVFSKDSFSRKKDKSNINTIERDEFLEWTKSI
                     WSFPTVSAKKIGHPAPFPVELPYRLIQLYTFKDDVILDPFCGSGSTCIAAIKSNRHYI
                     GYDIKEEYV"
BASE COUNT         1247 a          573 c          746 g         1014 t
ORIGIN      
        1 aatatgaccc tgagtttctg gaggagctgg agagtgaaca tacaatatat gcttatgatg
       61 aaaaccaaca atttagagga gtgaataaga aaggccggga catatatatc tttgataatg
      121 gttactgcct aaccgctgct aagttttgtg aatttgtgcc taagctccag agaatgggag
      181 ggggtgaggt cacggatcat ttacaaagtg agatctttat gataaataat ggcctggtta
      241 gtgactggat gggagaaaag aaaactaaac tcctggagaa agcccttaag tgtaccacct
      301 gccagctcct gcataatgat tttgttacct atggcatcct aaacttagta cttaaggacc
      361 gttacggggt aactatggag accgataatt ttggtaagat actttctgaa aatgacttta
      421 taattgagga cgtggaggag gagctccaga ggatagcctc ccagaagctg gaaaaggata
      481 aaaaagagtc ataagaaaca accctaaaag caccgtttag tatatatcaa aaggccactt
      541 tatagtactt tagaggtaac tcccagggcc taacctatga aaaaggccgt attaaacccg
      601 gcaggtctcc cgggacctgg gaggcataag cccctttaat gtctttatcc ctggaggcca
      661 ccggtggccc ttttaccctc ttttttctag gtttaatcta ggtttcttta aggtttattg
      721 taaggtcatg gtaatgttta gacctctatt ttactatata tgaaagatac tttttgaaaa
      781 ctttaaatcc ctgggaggcc gttttacccc tgaaagggcc gtatttatcc cggcagggat
      841 cagggttact atacatgaga ggggcataag ctactaaaaa agtataaccc tcccctccct
      901 accaggttta aagataatct ccagttataa aaggggtggc ccctctctcc caggttatta
      961 gttttcacgt gcttttataa tatctacaat aatattttta cgtaatttct aataacaccc
     1021 taaaagatat tagttttcac gtgaaattat aatataacag tattagggag gtgggagtag
     1081 tgagagagta taagacccta gtaaaatgtt atcaacgtaa aaaaagccgg cagcacctaa
     1141 taactcttaa gggagatcat ggatttaagg gagatgaccc cgtggtggta cttcctgagg
     1201 aggattttaa tgatttagtg accctccagg ataaagcccg ggatcatgat catgagctac
     1261 ttttaaagga tgaggagata tcccggttaa aactggatct ccagggctcc agggatgaga
     1321 tagagagtaa ggcccgggat cttatggaac atgaaagccg ggttagtgta tatgaagata
     1381 acattaaaca ccttaaagag gacctccagg ccttaaagga taagaacctt caattagagg
     1441 agatcctcaa ggcctcccgg gatgaaatag gggagcaccg tttaaggatc cgtgaacttg
     1501 aaaagctgga gctggaagac taccagggcc tatataataa acttagaaat aagcatgatc
     1561 acctccagga gcgttttaat aaatccctgg aggaggtaaa cggcctccaa cggataataa
     1621 gtgacctttc taataggggt tttattgatt acattacagg acgtttacca gaaagttaca
     1681 aacaactaag tgagccgaaa aaagcaactt aacaacataa taataatatt atttagttag
     1741 ggggtttaaa gtgctgaatt agtaagattc atagaaatcg cttttggaat tgtatttgaa
     1801 aaaaatttat cttaaaacaa aagaaaggag agaacaaatc atgggaaatc acgatctgtg
     1861 gattaaagat accgaaacta gatttttcgc tgaatcgagg agctttgcaa cccctgaaca
     1921 attattttat gtaacagatg atcaaaggta tttatcttac tggcctaaag ggtacaaagg
     1981 taaaaaaagt acattacagt caagaaattc tttaatcggc aatttcacag aaaagtggac
     2041 tacagatctt attcaaaaag ttgttaacga taaaggactt tatgctgttc aaggagccgt
     2101 ttgtgatgaa attgctctaa gtaagagatc ccctgctgat gttgtaatat ctaaaaagaa
     2161 agctatcaac ttaaaaccag aagacatcgt gctaatcata gaagtaaaaa tgtcagttgt
     2221 gtggaactgg caattgagta ttaatccttc atgtgaagaa ctggtttgta ttggtaatta
     2281 caaaacacat caaggtaacc ccgggctttt aagatctgat tccatgttaa aagctattgg
     2341 taagagcatt aatattcgag tttcaagctt caaagcgtct aatatcccta taataatttt
     2401 gggaaatact ccaattacag atagctacta ttccaaagta gaccatcttc aaaaggctgg
     2461 tattatacaa ggattttggt ctgttaatcc agatcctctg gatggtgcgg aatcaattaa
     2521 agaaacagaa gaacaaggtt tctatagatt tgacaatttc gaagaattaa aaacttcatt
     2581 tgatttgttg ttagaagaag aattaaactt tttctcaagt atgaaaacca aaaatgaact
     2641 tggacaaatt attgaattat ccgacaagga agaaacttat gaaagaaaag ctgaagtatt
     2701 tttagatttg ataagggagt agtctgatgt caactaaaag aaaagaacgt aaagggacta
     2761 aaacaagttc ttttggaagc tcaggtagag caaatcatga ttcatctgag ttttatggga
     2821 gtaaattata ctctgaaata gaaattccta agaaagtaaa attcattgaa aatgaaatag
     2881 ccccaagtaa acttgataaa atttattgta aatcaagtga aaaaatggag gaaattcctg
     2941 ataatagtgt tcatttaatg gtcacatccc ccccttataa tgtaaaaaag gagtatgatg
     3001 aggacctgac actaagggaa tacatggacc tcttgaggaa catcttccaa gaaacttata
     3061 ataaattagt aacaggagga agggtttgta taaatattgc gaatgtaggg agaaaaccat
     3121 atgtcccttt acatgctcat ataattgata ttatgctgaa tatcggattt ataatgaggg
     3181 gagagataat ttgggataaa tctgcaagtg caggttcttc atgtgcgtgg ggcagttgga
     3241 aatccgcttc aaatccagtt ttaagagatg ttcatgaata tattcttgtc ttttcaaagg
     3301 attctttttc aagaaagaaa gataaatcaa atataaacac tatagaaaga gatgaatttt
     3361 tagaatggac taaaagtata tggagctttc caacagtttc agcaaaaaaa atcggacacc
     3421 ccgcaccatt cccagttgaa ttaccttata ggttaattca gttatacaca tttaaagatg
     3481 atgttatatt agatcctttt tgcggtagtg gttcgacttg catagcagct ataaaatcta
     3541 atagacatta tattggttat gatatcaaag aagagtatgt
//
LOCUS       DAOQVW010000119         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29681_length_3576_cov_3.338540, whole genome shotgun sequence.
ACCESSION   DAOQVW010000119 DAOQVW010000000
VERSION     DAOQVW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29681_length_3576_cov_3.338540"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="PKK85_03575"
     CDS             <1..1713
                     /locus_tag="PKK85_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HNS25129.1"
                     /translation="FRRCVREAGLNPFLFEFANIREHDSWVHMAEPEAATEKAKDLVR
                     MAVAKARLLEALEASKVPVDNKALVIGGGVAGIQSALDLADMGFKTYLVEKQPTIGGR
                     MAQLDKTFPTLDCSMCILAPKTVDVGKHENIELISYAEVKEVNGYIGNFQVVIEKKPR
                     YVLEDICIGCGSCTEVCPIEMPNYFDEGMGMVKATFIPFPQAVPLVATINKDYCIDCK
                     LCDQACGNGAIFHEQEAEEIEIEVGTIIVATGYDPYDPTEKKEWSYADAENVITGLEL
                     ERLINASGPTMGKVLKPSDGEKPKSVAFIQCVGSRDAQIDKPYCSRVCCMYAMKNAQL
                     IKDKMPDTDVAIYYMDIRAFGKGFEEFYQRSQEKYGIKFIRGRPATVLVNPDDTLTIR
                     SEDSMLGKITEYNYDMVVLSVGLQPPEGSEELRQTIGLSKSADGFLMEAHPKLRPVDT
                     LTDGVYLAGVSQGPKDIPDAVAQASGAAARAAIPMVKGEVEIEPIIAAVDSEVCGGCE
                     ICLELCPFGAIDRVDDQAQVNVALCKGCGTCVAACPSGAMDQQHFRTSQIMAQIEAAL
                     NAGK"
     gene            2072..3340
                     /gene="glyA"
                     /locus_tag="PKK85_03580"
     CDS             2072..3340
                     /gene="glyA"
                     /locus_tag="PKK85_03580"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNS25130.1"
                     /translation="MVKNEEYAQKIKEITKKHHEWMKNSINLIASENITSTSVREALS
                     SDLSHRYAEGLPGKRFYEGCQYIDQIEEMTIALSKKLFQAEHANVQPISGVVANLASF
                     FAVSGQGDAIMALDVPVGGHISHAPVSAAGIRCLDVHPHPFDPDNMNIDADAMKKSIL
                     EEKPKIVLLGGSLFLFPHPVETAREAADEVGAKVMYDGAHVMGLIAGGHFQDPLKEGA
                     DLLVGSTHKTFPGPQGGIILCKKDIAEVVDDAVFPGVVSNHHLHHLAALGIATAEMLE
                     FGADYAHQTIKNAQKLGESLYELGFNVLCEDQGFTKSHQVAMDVSNISHASILAKTLE
                     KNNIILNKNLLPWDDVNRTDDPSGIRMGTQEITRRGMKESEMVEVAEYIKRVVMDGET
                     VEDEVTEFMGQYTQVHYAFQPDDAYRYLEF"
     gene            3362..>3576
                     /locus_tag="PKK85_03585"
     CDS             3362..>3576
                     /locus_tag="PKK85_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNS25131.1"
                     /translation="MKIAWGITGAGHLLLESVEIVENLSREHEVTVLLSGAGEEVLKM
                     YGLYNRVESITGGYYRELVLEKDQKFS"
BASE COUNT         1068 a          731 c          852 g          925 t
ORIGIN      
        1 ttccgaagat gtgttaggga agctggactg aatccattct tatttgaatt tgctaacatt
       61 agggagcacg actcctgggt gcacatggca gaaccagaag ctgctaccga aaaagctaag
      121 gacctggtcc gcatggcagt ggctaaggcc cgtttgttag aagctctgga agcctccaag
      181 gtaccagtgg acaacaaggc cctggtaatt ggtggaggag ttgcaggtat tcaatctgca
      241 cttgatctgg ctgatatggg attcaaaact tacctagttg aaaaacagcc cacaattggt
      301 ggtcgaatgg cacaactgga caaaactttc cctaccctgg actgttccat gtgtattcta
      361 gcccctaaga ctgtggatgt tggtaaacac gaaaacatcg agttaatctc ctacgctgaa
      421 gtgaaagaag ttaatggtta cattggtaac ttccaagtgg taattgagaa aaaaccaaga
      481 tacgtacttg aagacatctg tattgggtgt ggaagttgta ccgaggtttg tccaatagaa
      541 atgcccaact acttcgatga aggtatgggt atggtcaaag cgaccttcat accgttccct
      601 caggcagtgc cactggtggc caccattaac aaggattact gtatcgactg taaattgtgt
      661 gatcaggcct gtggaaatgg tgccatcttc catgaacagg aagcagaaga gatcgaaatt
      721 gaagtgggta ccataattgt agccactggt tacgacccct acgaccccac agagaagaaa
      781 gaatggagct acgctgatgc cgaaaacgtg ataactggtc tcgaactgga aagacttatc
      841 aacgcatctg gaccgaccat gggtaaagtt ttaaaacctt ctgatggtga aaaacccaag
      901 agtgtggctt tcatccagtg tgtgggtagt cgtgatgcgc agatcgacaa accatattgt
      961 tcccgtgtgt gctgtatgta cgccatgaaa aatgcccagt tgatcaagga taaaatgcct
     1021 gacactgacg tggccattta ctacatggat attcgggcct ttggtaaagg attcgaagag
     1081 ttctaccaaa gatctcagga aaaatatgga attaaattca tccgcggccg acccgcaacc
     1141 gtattggtca acccagatga caccctcacc atccgctctg aggacagcat gctgggcaaa
     1201 atcaccgagt acaactacga catggtagtg ctgtcagtgg gtctacagcc cccagaagga
     1261 tcagaagaac tccgacaaac tattggtctc tccaaaagtg ctgatggttt cctgatggaa
     1321 gctcacccca aactgcgacc tgttgatacc ttaactgacg gagtatacct tgctggtgta
     1381 tctcaaggtc ctaaagacat tccagacgca gtggcccagg cttcaggtgc cgccgcacgt
     1441 gctgccatcc ccatggttaa aggtgaagtg gaaatcgaac ccatcatcgc tgctgtggac
     1501 agtgaagtct gtggtggatg tgaaatctgc ttggagctat gtcccttcgg agctattgat
     1561 cgagttgatg accaggcaca agtcaacgtt gcactttgta aaggttgtgg aacctgtgtg
     1621 gctgcctgtc catcaggagc aatggatcag cagcacttcc gaacctctca aataatggct
     1681 cagattgaag ctgccttaaa cgcaggtaaa taagggttta atcccttatt tttttatttt
     1741 cttttaatta gacccctaaa acaatccata agtatcatat attttaaact aattctattt
     1801 ccccaagtat tgatggatta attcagattc ttaagctgga atttgaaaaa gtaatggttg
     1861 atatcctatc tgcgaagtgg aggatatagt cgctaatatt cagaaaataa agactaatcc
     1921 tcattcgaag gaccatgtga gtctttgaat tattatttga atctgcatgg agggacttgc
     1981 aaagtattta taactccaat ttttatagtt aatttcacta tgaatttaac aattgataat
     2041 tcaatttttt cagctttatc ggaggaatca gatggtcaaa aacgaagaat atgcccaaaa
     2101 aataaaggaa attactaaaa aacatcacga atggatgaaa aatagtataa atctaattgc
     2161 cagtgaaaac ataacaagta ccagtgtgag agaggcattg tcaagtgact tatcccatcg
     2221 ttacgccgaa ggacttcctg ggaagcgttt ctatgaaggt tgtcagtaca tcgaccagat
     2281 agaggaaatg accattgcat tatctaaaaa attattccag gccgaacatg ccaacgtaca
     2341 gcccatatct ggagtggttg ctaatttagc gtcctttttt gcagtatcag gtcagggtga
     2401 tgctatcatg gcattggatg ttcctgtggg agggcatata agtcatgctc ctgtgagtgc
     2461 agctggaata aggtgtcttg acgtgcaccc ccaccccttt gaccccgata atatgaacat
     2521 cgacgccgat gccatgaaga agagtatctt ggaagaaaag ccaaaaattg tcctcttagg
     2581 aggtagtcta tttctattcc cccatcctgt tgaaactgcc agggaagcag ctgatgaggt
     2641 gggggctaaa gttatgtacg atggagcaca tgtaatgggt cttatagctg gaggtcactt
     2701 ccaagatcca ctgaaagagg gtgcggacct tctagtagga agcacccata aaactttccc
     2761 cggtccacag ggcggcatta tactctgtaa aaaggatata gcggaggttg ttgacgatgc
     2821 agtgttccca ggggttgtta gcaaccatca tctccatcat cttgctgcac tgggcatagc
     2881 caccgcagag atgctggaat ttggagcaga ctacgcccat caaaccatta aaaatgccca
     2941 aaaactaggt gaaagcttgt atgaactcgg cttcaatgta ttatgtgaag atcagggatt
     3001 taccaaatcc catcaagtag cgatggatgt ttccaacatc agtcatgcct caatactggc
     3061 taaaaccttg gagaaaaata atatcatact caacaagaat ttacttccct gggatgatgt
     3121 caaccgtacc gatgacccct ctggaattcg tatgggaacc caggaaatta ctcgccgggg
     3181 aatgaaagaa tctgaaatgg tggaagtggc tgaatacata aaacgtgtag tgatggacgg
     3241 tgaaactgtg gaagatgaag tcacagaatt catgggccag tacacccagg tgcactatgc
     3301 gttccagcca gatgatgcct atcgctactt agaattttaa aaaaaatctg gagaaagttg
     3361 tatgaaaatt gcttggggta tcaccggagc aggacattta ctcctggaga gtgttgaaat
     3421 agtagaaaat ctttccaggg agcatgaggt aacagtgcta ctatctggtg cgggtgaaga
     3481 ggttctaaaa atgtacgggc tttataaccg ggttgaatct ataactgggg gttattaccg
     3541 ggaattggtt ctggaaaagg atcagaagtt cagtta
//
LOCUS       DAOQVW010000120         3575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_29690_length_3575_cov_4.734091, whole genome shotgun sequence.
ACCESSION   DAOQVW010000120 DAOQVW010000000
VERSION     DAOQVW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29690_length_3575_cov_4.734091"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            577..>3575
                     /locus_tag="PKK85_03590"
     CDS             577..>3575
                     /locus_tag="PKK85_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS25132.1"
                     /translation="MSVTKILVVEDEGLTAMELQRKLRYWGYDVPTFAFSSKEAVQKA
                     KKIHPDLVLMDIVLKGEGDGIDAAQRIKDLFDIPIIYLTAHVDEKTRERAERTKPYAY
                     LVKPFEEKELHQKVEEALYSHKAEKRLIESGKWVDKKLTNSGVIVADNKGCVRFLNDK
                     AARLTGFNPSDALFKHLSEVLPISIIKKVENSDNYLNNLINAYSTESERTTLITPENE
                     KILIKYNIAPIEDDEKKILGVNLIFEDVTQEVKNEEALLKSEKKFRGIFEQSPLALAV
                     YDFEGKLIGANQACLEIGGAEDLDELRSSTLFKDLKINEKEYKILNSGNKVSYEIELE
                     LGDDLDYEKTDSGQTCLEVLISSIQLDNTNKGYILQFKDVTTHKIVEENLQKTCEDYQ
                     DVLESIDNSVVVLDSEFNSKYLNQTAIDLSPLSAHEVKGKTLERIFPFLYDEEVHETL
                     KMAAESEIPQKITKKCKVNHEDLYLHLEFFTSLNGLNIIITDITQQKINENQLKSSES
                     LYRSIVHEQNDIICRFTPEAKLTFTNPSYKLYFGKQPSGSRVFSISSTEQDKFMGMMN
                     SLTEESPVTIFEGPLEVADGDLRWWQWIIKAIFNENNTISEYQAVGREITPHKEKEES
                     MEAQLHQFALEIEKSQLEFEADKTTLESEIKQIRTKSEQLEKVRSELENKLQEKSDEL
                     SETKISFNKQLKSLEKRENEQINLNGKLKSELKTLQADFNKSWEEFEAELAAQKQSEL
                     ELQEKYHQMEMELQRTNVKLKETISKLHDTIENKNKALNNKNLELKRLEESSQLEIGT
                     LKEKDLQSRKALEKREKLLKNLYHRFRNNVNMISSLNSLQSEYMMDQMVSQFQENRNH
                     MKAVALVHQKLYQSPDLENIDFKEYIQNLTSYVKRSSGVRGVTIDIDAPEVLMDMDTA
                     IMCGMIVNELVSNSLKHAFPLQQEGSIRIQAQKNDHELSITVADNGIGLPEYFDLEKA
                     ESLGIKLVNTFIEQVSGQIIINSGAGT"
BASE COUNT         1336 a          606 c          674 g          959 t
ORIGIN      
        1 ggtgcctttg atgaagatct ggttgtccag gccctgcacc tcccaccagg gttacgtcca
       61 gtgtccctga tacccatggg ttatcctgat gaaaatccat cacccccgcc tagaggggat
      121 ttggaagaat tcatacacta tgaaaggttt aaataaacta atttttttta atatatcaaa
      181 ttcactaccc ggcagaatat ttttcttttt aaaaaagccg gattttttta tttcaccctg
      241 gtgatgttac ctataatata ttatgggtag ggttatgggc aggggaaatt ccatccagaa
      301 tttttattgt gaataggtgt tttttttctt gagatcttca gttaacagat catggcattc
      361 aataattgtt cttgaaatac atttaaagat tttattctca gattaaatgg attttataac
      421 atatttttta atataaaaga ttgttatctg catttaatcc aagaaatttt gcacagaaat
      481 ttcccaacaa atgaaaagct tttatatagg gtcgagcata attaaaagta caagtcaata
      541 ttgttcacga tgaacaatat tttccgaggg aaaaaaatgt cggtaacaaa aattctcgtg
      601 gtggaagatg aaggtttaac tgccatggaa ctccagagaa aattacgata ctggggttat
      661 gatgtcccca catttgcatt ttcaagtaaa gaagccgtgc aaaaagctaa aaaaatccac
      721 cccgacttgg ttttaatgga tatagtcctc aaaggggaag gagatgggat agacgccgcc
      781 cagagaataa aggatctatt tgatattccc ataatatatc tcactgcaca tgtagatgaa
      841 aaaacccgtg aaagggcaga aagaaccaaa ccttacgcct atttggtaaa gccattcgag
      901 gagaaagagc tacatcagaa agttgaagaa gccctttaca gtcacaaagc agaaaaaaga
      961 ttaatagaaa gtggaaagtg ggtagataaa aaattaacaa attcaggagt aattgttgca
     1021 gataataaag gatgtgtgag atttttaaat gacaaagcag ctagactgac tggattcaat
     1081 ccatcagatg cactattcaa gcatctatct gaagtgcttc ccataagtat cattaaaaaa
     1141 gttgaaaact ctgataatta tctgaacaat ttaataaatg cctacagtac agaatcagag
     1201 agaaccactc taataactcc tgaaaatgaa aaaatcctga tcaagtacaa tatcgccccc
     1261 atcgaagatg atgaaaagaa aattctggga gtaaacctca tctttgagga tgtcacacag
     1321 gaagttaaga atgaagaagc tttactaaaa agtgaaaaaa agttcagagg gattttcgaa
     1381 caatcacccc tggcactggc agtctacgat ttcgaaggaa aattaattgg tgccaaccag
     1441 gcttgcctgg aaataggggg tgctgaagat ttagatgaat taaggtcgtc aacacttttt
     1501 aaagacttaa aaataaatga aaaggaatat aaaatcctta attctggtaa taaagttagc
     1561 tatgagattg aattagaact aggggatgat ctggactatg aaaaaactga ttctggacaa
     1621 acctgtttag aagtgttaat aagttctatc caattagata acactaataa aggctatatt
     1681 ttacaattta aagacgtaac cacacataaa attgtagaag aaaatctaca gaaaacatgt
     1741 gaagattatc aggatgtttt agaaagtatc gataacagcg tagttgttct ggattcagaa
     1801 ttcaactcaa aatatttaaa ccagacagca atcgatttat cgccgttgtc tgcccatgaa
     1861 gtcaagggaa aaactctgga aagaatattt ccttttcttt acgatgaaga agtacatgaa
     1921 actctaaaga tggccgcgga atctgagatc ccacaaaaaa taaccaagaa atgtaaagtt
     1981 aaccatgaag atctgtatct gcatttagaa ttcttcactt cactcaatgg tttgaacatc
     2041 attataactg atataactca acaaaaaatc aatgaaaatc aattaaaaag ctcagaaagt
     2101 ctctatcgat ccattgtcca tgaacaaaat gatatcattt gtcgtttcac tccagaggcc
     2161 aagttaactt ttaccaaccc ttcctacaaa ttatattttg ggaaacaacc ctctggaagt
     2221 cgggtatttt ccatatctag cactgaacag gataaattca tgggaatgat gaattcattg
     2281 actgaagaaa gtccagtgac catttttgag ggtcctttgg aagttgctga tggtgattta
     2341 agatggtggc agtggataat caaggccatc ttcaacgaaa ataatacaat ttcggagtat
     2401 caagctgttg gtcgtgaaat cactcctcat aaagaaaaag aagaatctat ggaggctcaa
     2461 cttcatcaat ttgctctaga aatagaaaaa agtcagttag aatttgaagc agataaaact
     2521 actctggaat ctgaaattaa acagatcagg acaaaaagtg aacagctaga aaaagtcagg
     2581 agtgaactgg agaataaact acaagaaaag tctgatgaac tttctgaaac taaaatatca
     2641 tttaataaac aactgaagtc ccttgaaaaa cgagaaaatg aacaaataaa tcttaacggc
     2701 aaactaaaat cagaactaaa aacacttcaa gccgatttta ataagtcctg ggaagaattt
     2761 gaggccgaat tggcagctca gaaacaatca gaacttgaac ttcaagaaaa atatcatcag
     2821 atggagatgg aattacaaag aacaaatgta aaactcaaag aaaccatttc taaactacat
     2881 gataccatag aaaataaaaa taaggcgctt aacaataaaa atttagaatt aaaacgctta
     2941 gaagaatcat ctcaactaga gataggtact ttaaaggaaa aggacctgca aagtagaaaa
     3001 gcgctggaga aaagggaaaa acttttgaaa aatctctacc atcgtttccg taacaatgtg
     3061 aacatgattt ccagtttaaa cagtttacaa tcggagtaca tgatggatca gatggttagt
     3121 cagttccagg aaaacagaaa tcacatgaaa gccgttgccc tggtacatca aaagctttat
     3181 caatccccag acctggagaa catagatttc aaggaataca tccaaaacct taccagttac
     3241 gtgaagagat cttcaggagt taggggggtt acgatagata ttgatgctcc tgaggtattg
     3301 atggatatgg acaccgccat aatgtgtgga atgattgtta atgagcttgt atctaactcc
     3361 cttaaacatg ccttcccctt acagcaagaa ggttccatac gcatccaggc acaaaaaaat
     3421 gaccatgaac tatccataac tgtggctgac aatggtatcg gcctacccga gtattttgac
     3481 ctggaaaaag ccgaatccct ggggataaaa ctggttaata cttttattga acaagttagt
     3541 ggccaaataa ttattaacag cggtgccggc acgga
//
LOCUS       DAOQVW010000121         3523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_30341_length_3523_cov_3.233852, whole genome shotgun sequence.
ACCESSION   DAOQVW010000121 DAOQVW010000000
VERSION     DAOQVW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30341_length_3523_cov_3.233852"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..603
                     /locus_tag="PKK85_03595"
     CDS             133..603
                     /locus_tag="PKK85_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNS25133.1"
                     /translation="MKTLIACYSYTGNTMVIAQKINEAIDSDLTRIQPVKDRWYMIKA
                     IHAYLEKKWPIEDCIKDLYEYDCLLLCCPIWAGRAPPGVNQYLEELQNVKGKKLAVMV
                     TMGGNGNEIATRQIKTALEVKGMELIARLKITGNDQKSGAWVAKTEEFCQIFMK"
     gene            complement(1049..1879)
                     /locus_tag="PKK85_03600"
     CDS             complement(1049..1879)
                     /locus_tag="PKK85_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HNS25134.1"
                     /translation="MVVKIGVIKCGNLGTSPVIDLVLDERADRPNIDVRGVGSGAKMN
                     PEQIEEAVPKIMDFEPDFVVFISPNPGAPGPARARELLSEMDVPAVIVGDAPGMGKRE
                     EMDEQGLGYIIVLGDPMIGARREFLDPTEMASFNADVIKVLAATGAYRVVQETLDGII
                     ATCEAGNDVELPKVVIDAAKATDAGGFSNPYAKAKAMAAYEIAAKVGDVDLKGCFMVK
                     EMEKYVPIVASAHEMIAAAAKLAVEAREIEKANDTVLRKPHGGEGQTLSKTELVSKPQ
                     "
     gene            complement(2510..>3523)
                     /locus_tag="PKK85_03605"
     CDS             complement(2510..>3523)
                     /locus_tag="PKK85_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase family
                     protein"
                     /protein_id="HNS25135.1"
                     /translation="YPEPATFGYIMLGTLFFLLGVIVPWLIYRYFGRLKIFFNKQGTE
                     LGDDPELYSRLKFLVNEKLLLGIVIFSLLLQGINLYLLGGIPLLSGYLKFRATTDLWR
                     IAYPLFLPALNILIAKYPRKIYYLLFMVGVVLFGLTGYRTTTLAIIISVFITTYYTRR
                     LKTSHIIGFIIFTAIIGIAVGYIAVKAIEWQQWSLNPLDLVFYRAAFTTMVLSKIVAI
                     QGTTGGDLFLQIFSSGHPRVTVGCTALGYRACITSTIFGPALLDFGAVGMAIQMFLIG
                     LALKMVYNVQKLKNNAYTAFYSIILAHTLIWIETGPTDISVWFFYFLALFAVIVFWFK
                     YSK"
BASE COUNT         1094 a          701 c          716 g         1012 t
ORIGIN      
        1 atagggcatg ggaagttcaa gggacaataa ttcgactgac tgcggaggca ttttaacgaa
       61 agaatgttta gcaccgaaaa ttaaggattg gctaagtatt attattagtc aaaaaaatct
      121 ggaggtataa atatgaaaac cctaatagcc tgttattcat acacgggaaa taccatggta
      181 atagctcaga agataaatga agctatagat tcggatttaa cccgaatcca gccagtaaaa
      241 gatcgctggt acatgataaa ggcaattcac gcctatctgg aaaaaaaatg gcccatagaa
      301 gattgtatta aggatctgta tgaatatgat tgtttgcttc tatgctgccc catctgggcc
      361 ggccgcgctc cacctggtgt gaatcagtac cttgaagagc tgcaaaatgt gaaggggaaa
      421 aaactggctg tcatggtcac catgggtggg aatggaaatg aaatagccac caggcaaatc
      481 aagactgctc tggaagttaa gggaatggaa ctaatagctc ggctaaaaat aactggtaat
      541 gaccagaaaa gtggtgcttg ggtggccaaa acagaagaat tttgccagat cttcatgaaa
      601 taataatttc catagcatgg aatacttcaa ggatagaaaa aatagggtta ttctgcccaa
      661 aatttattct agacaccttg taattttcat ggaacagccg atattggctt ccacctcgaa
      721 gtatgttaat ccatgttttt ctgataacta agactcttat acgccaccca tctcccctga
      781 cccccatctt cggatttagt cctcttgtaa gtaataacta ttttatggac tcagtttatc
      841 aagttctttc tctaagccgt gggatttgat cgttccatca tgttttatga gatcatacca
      901 ccatttggtt ccactcttca tgagctaaat agttatttaa aaacaagaca ataccgtccc
      961 gtgatcggaa ctgaatatta tgataggttt aagaaaattc taaaaaaaca aaaatatgca
     1021 gtcccaggga ctgctatttt attaggtttt attgtggttt ggacactagt tctgtcttgg
     1081 acagagtctg gccttctcca ccgtgtggtt ttcgtagcac agtgtcgttg gctttttcta
     1141 tttcacgtgc ttccactgct aatttagctg cagctgcaat catttcgtgg gctgaagcca
     1201 caataggaac gtatttttcc atttctttaa ccatgaagca gcctttaagg tctacatctc
     1261 ccactttagc agcaatttcg taagcagcca tggctttggc tttagcataa ggattagaga
     1321 atccacctgc atccgtggct ttggcagcgt cgatcactac ttttggtaac tctacatcgt
     1381 tgcccgcttc gcaggtagct atgatgccat ccagggtttc ttgaaccacc cggtatgctc
     1441 cggtggcagc taggaccttg atcacatcgg cgttgaagct ggccatttca gttggatcca
     1501 ggaattctct tctagctcca atcatagggt ctcccaggac aataatgtat cctaggcctt
     1561 gttcatccat ttcctctctt tttcccattc ctggagcatc tcccacaata acagctggaa
     1621 catccatttc agataagagt tctcttgctc gcgcagggcc gggagcacca gggttggggc
     1681 taatgaatac cacaaaatcg ggttcgaagt ccattatctt gggtacggct tcttcgatct
     1741 gttctgggtt catctttgca cctgagccca ctcccctaac atcgatgtta ggtcggtccg
     1801 ccctctcgtc taacaccaag tcgatgaccg gagaggtacc taggttaccg catttaataa
     1861 ctcctatttt aacaaccatt ttatcaccgg tgatgctatt ttgatgatct aataagtatt
     1921 ttttcattta attttttcaa aaaggttttt atacccatat tctctgcagt atacagtaac
     1981 taagtcgttc aaaagagata aacaaagacc atttaaatat atttcagtaa aataatcaaa
     2041 attcaaactt ccccatttat tttttccaat attataccgt gtcaacataa atcggcgtcc
     2101 taaataattt ttggaccatt tttttttgga agagtgctgt gtagtaggtg tacttagacc
     2161 accatttcaa gtgtaacacc ggaataatat aaaaaatttt tataaaactg ccccagtcat
     2221 cagccatttg atatgtataa acatgaaata tcaaggttgt aaggcaacat acgaactatt
     2281 attaaaaata gatttattga ctaaaaagct taattgacta ttgttagtgt attgaatatc
     2341 aaataaggag ctattgttaa atatatctat gcataaaatg attttatcaa gtattattct
     2401 acagtagaaa aaaaattggt gaaagtactg taataaattt ctacagtaga aattttgggg
     2461 cctattcatt tttttgatta gaaaaaaatg gtcccattta attttaaatt catttagaat
     2521 acttaaacca gaatacaatc accgcaaaaa gtgccaggaa atagaaaaac caaactgaaa
     2581 tatcagttgg gccggtttct atccatataa gagtgtgggc tagaattata gaataaaatg
     2641 cagtgtaagc attattttta agtttctgta cattataaac cattttcagg gctaggccaa
     2701 tgaggaacat ctgtattgcc atgccaacag caccgaagtc caggagtgct ggaccaaaga
     2761 tggtggaggt gatgcaagcc cggtacccca gggcagtgca gcccaccgtg actcttggat
     2821 ggccagagga aaatatctgc aggaaaaggt caccaccagt ggtgccctgg atggccacaa
     2881 ttttggagag aaccatggtg gtgaaagcgg cccgataaaa aacaagatcc aaagggttca
     2941 aactccactg ctgccattct atagctttaa ctgctatgta tcccactgca ataccaataa
     3001 tggcagtgaa aataatgaat ccaatgatat gagaagtttt aagacggcga gtgtaatagg
     3061 tggtgataaa tacgctgatt ataatggcca gggttgtggt gcggtagccg gttaggccaa
     3121 acagcaccac tcccaccata aataggagat aataaatttt acggggatat ttagctataa
     3181 gaatgtttaa tgctggtaaa aatagggggt aagctatccg ccatagatca gtggtggccc
     3241 tgaacttaag gtatccactc aagaggggga tgcctcccaa aagatagagg ttgatgccct
     3301 gcaggagaag actgaaaatc acgattccca gtagcagttt ctcgttaact aaaaatttca
     3361 accgggaata caattcagga tcatcaccca attccgtgcc ttgtttattg aagaagattt
     3421 tcagacgccc aaaatatcga tatatgagcc agggaacaat cactcccagt aagaagaaca
     3481 gagtacccaa cattatgtag ccaaaggtag ctggctcagg ata
//
LOCUS       DAOQVW010000122         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_30386_length_3520_cov_3.359019, whole genome shotgun sequence.
ACCESSION   DAOQVW010000122 DAOQVW010000000
VERSION     DAOQVW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30386_length_3520_cov_3.359019"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PKK85_03610"
     CDS             complement(<1..147)
                     /locus_tag="PKK85_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNS25136.1"
                     /translation="MRVNLVLELLDVPGQLLDVLRPMGRLGANIVAVIHQRDVKTDEG
                     KVPVQ"
     gene            complement(175..390)
                     /gene="gatC"
                     /locus_tag="PKK85_03615"
     CDS             complement(175..390)
                     /gene="gatC"
                     /locus_tag="PKK85_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn) amidotransferase subunit GatC"
                     /protein_id="HNS25137.1"
                     /translation="MKIEKEAEEILKKFSEALQDIPELEETYYIVDNLNREREDLAHE
                     KDSTKLLRNSRVDEDGNVMVEKSKWTG"
     gene            complement(475..1929)
                     /locus_tag="PKK85_03620"
     CDS             complement(475..1929)
                     /locus_tag="PKK85_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNS25138.1"
                     /translation="MSGIAGLFGDLTGSWLTEMLKKLEHRGPDGSGIYVDGQLFHGET
                     KNLSRREFKVALGQNSLSNAGSEVQQPLVDEERVLVANGRIYNYQELKQSSNYNFRTN
                     IDWEVILSLLDEHGGQLFQAIPKVVERLDGDYAFVVFDGENWAAVRDPVGVKPLYYGA
                     HQGMDLFGVASERKALWEIGIREVDSLNPGFMLCNRELIPLPPRIKKSAPLDPVDNSS
                     HVWLKSKLKADLIRSVQKRIRGLDRVGVVFSGGVDSTILVQICLSLGVKPELYTVGRK
                     GSPDIRFSHEAADIMGLTLYTAQVTEEEVRRCVPLVLEAIEEWNIMKLGVGMATFMAA
                     EQAAENGLRVLLSGQGADELFGGYHRYLELCKEGPVALQNGLWQDLLQIYGVNLERDD
                     AVAMAHSVELRVPYLDLQVINRAMDIPTHYKIRDHNDQLRKCILREVAAELSVPEFIV
                     KRPKKAAQYGSGIHQLLVKKVFKDQRYVDKLKVG"
     gene            complement(1937..3313)
                     /gene="cysS"
                     /locus_tag="PKK85_03625"
     CDS             complement(1937..3313)
                     /gene="cysS"
                     /locus_tag="PKK85_03625"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNS25139.1"
                     /translation="MFIYSTLSRSKEEFSPLKPNRVKLFVCGPTVYDDSHIGHGRTYI
                     AFDCIARYFRYKGYSLFYLQNITDLDDKIIKRARERGVDPILLAREYERKYLADMKAL
                     GVENVNLHARATEHIPEIIEQIRKLLEKGFAYETENGVYFEEAKFPDFGKLSQRKLED
                     LTVHRVDPDPRKKSPGDFVLWKKIDEQPCWESPWGRGRPGWHIEDTAITEEYFGAQYD
                     IHGGGLDLIFPHHEAEIAQMESASGLKPMVLYWMHTGFLNVKGEKMSKSLGNFVTIQE
                     LLQRWDGEVFRFLVLSAHYRSPIDFGEDALIQAQNSLERIKKTLQKMVGFYEDPEKQG
                     GIKVGENRKIWDEVHKKFLEAMDDDFNTPQALSVLFEAIRYINRQLQDKSLKNSDIIK
                     IKTIFKDWGEILGVNFFPPEKKEDRTEDVLEILLDVRGKLREKKDWKLSDMIRSDLKD
                     LGFELEDS"
     gene            complement(3359..3429)
                     /locus_tag="PKK85_03630"
     tRNA            complement(3359..3429)
                     /locus_tag="PKK85_03630"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3395..3397),aa:Cys,seq:gca)
BASE COUNT          878 a          926 c          683 g         1033 t
ORIGIN      
        1 ctggactgga acttttcctt catcagtttt aacatctctc tggtgtatca ccgccacgat
       61 gttagcacca aggcgcccca taggtcttaa tacgtccaat aattgccctg gaacgtctaa
      121 aagttctaaa acaaggttaa ctcgcatttt attcacctta agtatcttag agtactatcc
      181 ggtccattta ctcttttcaa ccatgacgtt gccatcctca tcgaccctgc tgtttctgag
      241 aagtttagtg ctgtcttttt catgggccaa atcttcacgt tcccggttga ggttgtccac
      301 gatgtagtag gtttcttcta gttcaggaat gtcctggagt gcttcactaa actttttcaa
      361 tatttcctcg gcttcctttt caattttcaa aataattcct ccatcccaac ttggataagg
      421 tttgcatcaa atcagccacc ataataagtg tagaagttac ttttctatat attattaacc
      481 cactttcagt ttatctacgt atctttgatc cttaaaaact ttcttaacaa gtaactgatg
      541 gataccagac ccatactgcg ctgccttttt gggccgttta acaataaact cggggacact
      601 taattctgca gccacttccc gtaggatgca cttccgcagc tggtcattat ggtcacgtat
      661 cttatagtgg gtaggtatat ccatggccct atttataact tggagatcca gatagggaac
      721 ccgcagttcc acactgtggg ccatggccac tgcatcatcc cgctccaggt tcaccccata
      781 aatctgtaga aggtcctgcc acaatccatt ctgcagggca acaggcccct ctttacacaa
      841 ctcaagataa cgatgatacc caccaaagag ttcatccgca ccctgaccag ataggaggac
      901 cctcaaacca ttttctgcag cctgctctgc agccataaag gtggccatcc ccacccccag
      961 tttcatgatg ttccactcct caatggcctc caaaaccagg gggacgcatc ttcgcacctc
     1021 ttcttcggtt acctgggcgg tgtagagggt aaggcccata atatcagctg cttcatggga
     1081 gaatctaata tcaggggatc cttttctccc cacagtgtag agttctggtt taactcctaa
     1141 gctcagacat atctggacca ggatggtact atccacacca ccagagaata ctacacccac
     1201 ccgatccagg cccctgattc ttttttgcac agatcttatc aggtcagctt ttaacttaga
     1261 tttaagccag acatgggagg aattatcaac tggatcaagg ggtgctgatt tttttatcct
     1321 gggaggcagg ggaattagtt cacggttgca gagcatgaaa cccgggttta aactatccac
     1381 ctccctgata cctatctccc acagggcctt cctctcggag gccactccaa aaagatccat
     1441 cccctgatgg gcaccgtagt acaggggttt aactcccacc ggatccctca ccgcggccca
     1501 gttctcaccg tcaaatacca caaaggcgta gtccccatct aacctttcaa ctaccttagg
     1561 aatggcctgg aagagctggc caccatgttc atccaggagg gacagaatca cttcccagtc
     1621 tatgttggtc ctgaagttgt aatttgagga ctgcttcagc tcctggtagt tgtagatcct
     1681 cccgttagcc accagaaccc tttcttcatc caccaggggc tgctgaacct cagagcctgc
     1741 attggataat gaattttgac ccagagccac cttaaattct ctgcggctta gattcttagt
     1801 ttccccgtga aacaactggc catccacgta gatcccggaa ccatctggac cccgatgctc
     1861 cagttttttt agcatttcag ttaaccagga accagttaga tctccaaata atcctgctat
     1921 acccgacatc ccaatcttaa ctatcctcta attcaaaccc cagatccttt aaatcacttc
     1981 tgatcatgtc agatagtttc caatctttct tttcccttaa ctttccccgc acatccagca
     2041 ggatctccag cacatcctca gtccggtctt cttttttttc aggggggaag aagttaacac
     2101 ccaatatttc tccccaatcc ttaaaaatag tcttaatttt aataatatct gagtttttaa
     2161 ggcttttatc ttgtaattga cgatttatgt atctgatagc ctcgaataat actgataagg
     2221 cttggggggt gttgaagtcg tcatccatgg cttctaaaaa ttttttatgg acttcatccc
     2281 atatttttct attctccccc accttgattc ctccttgttt ttcagggtct tcatagaatc
     2341 caaccatttt ctggagggtt ttttttatcc tttccagact attctgggcc tggatcagtg
     2401 catcctcccc aaaatctatg gggctacggt aatgggcgga taaaaccagg aagcgaaaaa
     2461 cttcgccatc ccacctttgg agcaactcct ggatggtgac gaagttgccc agggatttgg
     2521 acatcttctc ccccttaaca ttcaagaatc cagtgtgcat ccagtagagt accatgggct
     2581 tcaatcctga tgccgactcc atctgggcaa tttccgcttc atggtgggga aatatgaggt
     2641 ccaatcctcc cccgtgaatg tcatactggg ctccgaagta ttcttcagtg atggctgtgt
     2701 cctcaatgtg ccagcccggc ctccccctac cccagggaga ctcccagcag ggctgttcat
     2761 ctatcttctt ccagagaaca aaatctccag gacttttctt tctaggatcc ggatccaccc
     2821 ggtgcacagt caagtcttcc aacttgcgct gcgatagctt accgaaatca gggaacttcg
     2881 cttcttcaaa atagactcca ttttcagttt cataggcaaa acctttctcc agaagctttc
     2941 ttatctgctc tattatctcc gggatatgtt ccgtggcacg agcatgtaga ttgacatttt
     3001 ccacacccag ggccttcatg tcagccagat atttcctttc atattcccgg gccagaagga
     3061 tgggatccac gcccctttcc cgggcccgtt ttataatctt atcatccagg tctgttatgt
     3121 tttgaagata gaacagactg taacccttat aacgaaaata gcgtgctatg cagtcaaagg
     3181 caatgtaagt tcgtccgtgg ccgatgtggc tgtcatcgta taccgtgggg ccgcaaacaa
     3241 agagtttaac tcgattaggt ttcaggggac tgaattcttc cttactccgg cttaaagtgc
     3301 tgtagatgaa catgataatc aatggaacaa ttttttttgt aatataaaaa agattcaagg
     3361 ccgagatcgg gatttgaacc cgagtatcat ggtctgcagc cacgcaccta gcctctcggt
     3421 catctcggca gcaatgatta ttatggtata gaagttctct taataaatta ttttcattgg
     3481 tgatctataa ggatgttgtg gttcatcaaa aagatatcat
//
LOCUS       DAOQVW010000123         3495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_30688_length_3495_cov_3.511337, whole genome shotgun sequence.
ACCESSION   DAOQVW010000123 DAOQVW010000000
VERSION     DAOQVW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30688_length_3495_cov_3.511337"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PKK85_03635"
     CDS             <1..459
                     /locus_tag="PKK85_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNS25140.1"
                     /translation="EIPRGSRNKYEYDKDMEAFALDRVLYSPVHYPAEYGIIPQTLYD
                     DGDPMDIMVLMEQPTFPGCIIESRAIGMMRMIDGGDQDDKILAVPTQDPRYQEVQDLE
                     DVAPHLLKEIAQFFQDYKALEGKETEVQGWVGKEEAFKAIKHSIELYKKM"
     gene            510..1075
                     /locus_tag="PKK85_03640"
                     /pseudo
     CDS             510..1075
                     /locus_tag="PKK85_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
     gene            1076..1258
                     /gene="spt4"
                     /locus_tag="PKK85_03645"
     CDS             1076..1258
                     /gene="spt4"
                     /locus_tag="PKK85_03645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HNS25141.1"
                     /translation="MVTKACTRCHRIVEGDLCEVCNLATSKNWSGFLIIVDPEKSHIA
                     RDLKIQEAGEYALRVR"
     gene            1258..1764
                     /locus_tag="PKK85_03650"
     CDS             1258..1764
                     /locus_tag="PKK85_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF359 domain-containing protein"
                     /protein_id="HNS25142.1"
                     /translation="MLVLKKNLRPEFKKAIGTLYPSLDDAEDFLASQEPDTLLVSVGD
                     VTTRNLQEQGLIPHLGIIDQQVERKPAPQDIVYDNVTLKADNPAGTVTRELWDAVEQG
                     FQLIKAGYRVLILVKGEEDLAVVPAVILAPPGSLVLYGQPGEGVVICEVDRLRDKVIK
                     MKEMLMEE"
     gene            1766..2071
                     /locus_tag="PKK85_03655"
     CDS             1766..2071
                     /locus_tag="PKK85_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HNS25143.1"
                     /translation="MEIDIKEKVENPLLNRTEIRFDCLYQGEATPKVLDVKNRLVAVL
                     NVDKDLLVVDKMKPSYGEGRSEGYAKLYDSPDHLSQVERNHVMVKNQEKTTPESEEE"
     gene            2091..2252
                     /locus_tag="PKK85_03660"
     CDS             2091..2252
                     /locus_tag="PKK85_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HNS25144.1"
                     /translation="MKKYQLYEVKDNKLVRKNPECVRCSHGVFMADHGDRYTCGKCGY
                     TQWKGKEKK"
     gene            2265..>3495
                     /gene="argH"
                     /locus_tag="PKK85_03665"
     CDS             2265..>3495
                     /gene="argH"
                     /locus_tag="PKK85_03665"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNS25145.1"
                     /translation="MNLRSGRFKGRMTSDAASYTSSLEFDPRIFSADVKCNRAHTTML
                     QEEGIISQETAHAITGSLDELEEKGISALDLDPSVEDIHMAVENYVTSRIGEKAGFMH
                     TAKSRNDQVATDLRISLKEEIEEIMGDLLNFIQNTVDMAEENLETVMVGYTHLQHAQP
                     TTFAHHLLSYAQALKRDYQRLGDAYKRVDLCPLGAAALTTTSFPINRQRTAQLLGFSH
                     VLENSLDAVSSRDFITETVSALAIMGVNLSRICEELIIWSTYEFGLVEIGDEYSSTSS
                     IMPQKKNPDVAEVARAKAISLQGELVTILSVTKALPQSYNRDLQEVTPHLWRAVDTAR
                     DTLNLVAEMLASVKFNHQRGLELASSNFSTATELADLMVRENQLPFRVAHQIVGRLVS
                     TALEEGLNPDLIDSQFID"
BASE COUNT         1001 a          829 c          927 g          738 t
ORIGIN      
        1 gaaatacccc gaggatcccg gaacaagtat gaatatgata aggatatgga agcctttgcc
       61 ctggatcggg tgctctactc accagtccat tacccggcag agtacggcat catcccccag
      121 accctgtacg atgacggtga ccccatggac atcatggtac tcatggaaca gcccaccttc
      181 cctggttgca tcattgaaag ccgggccatt ggtatgatga ggatgatcga cggcggtgac
      241 caggatgata agatcctggc ggtgcccacc caggaccccc gctaccagga ggttcaggac
      301 ttggaggacg ttgcccccca ccttttaaag gagatagcac agttcttcca ggactacaaa
      361 gctctggaag gaaaagaaac cgaagtccaa ggatgggttg gtaaagaaga ggctttcaag
      421 gctataaaac attctattga gctttataaa aagatgtgaa gcctgaggtt catggttaaa
      481 tcataagaag ttatctaaga gggatttgtt tgtatttagt atccaaaatt gaggacacgg
      541 tgcggatccc accccaccgt ttcgaggagc ctctgcagga agtggcagcc gagatcctca
      601 acaaaaacta catgggaaag atcgacaaga agatgggcct catggtcact gtcaaggaga
      661 ttgaggatat cggtgtgggc aaagtcatca tgggagatgg agccgcctac catgacgtgg
      721 tgttcaccgc cctgtttttc aaaccagaac tccacgagat cgtggtgggt gaggttatag
      781 aagtcactga cttcggtgcc tttgtacgca taggtcccat ggacggcctg gtacacgttt
      841 cccaggtcac tgatgattac ataaactacg atgccaagag aggcgcctcc tgggtaagga
      901 atccaagaaa agcttggagg agggcaacca tgtccggggc cgtatcgtgg ccttgagcct
      961 taaagaaaaa tccgccgaaa ccaaaatcgg actcaccatg agacaacccg gactaggtag
     1021 gctggaatgg atcaaggaag agaaagaaaa agctaaaaag aaggggaaaa gttaaatggt
     1081 taccaaagcc tgtaccaggt gtcaccgtat agtggagggt gacttgtgcg aagtgtgcaa
     1141 cctggccacc tccaagaact ggagtggttt tctgattata gtagaccctg aaaaatccca
     1201 catcgcccgg gacctcaaaa tccaagaggc aggagaatat gccctgaggg ttcgctagtg
     1261 ttagtcctaa aaaaaaattt gagaccagag tttaaaaaag ccattggcac cctttatcca
     1321 tccctggatg atgctgagga ttttctggcc tcccaagaac cagacactct cctggtttca
     1381 gtgggtgatg ttaccacccg gaacctgcag gagcagggac tgatccccca cctggggatt
     1441 atcgaccagc aggtggaaag aaaaccagcc ccacaggata tagtctacga taatgtaacc
     1501 ctgaaagcgg ataaccccgc tggtacagtg acccgtgaac tatgggatgc tgtggaacag
     1561 ggatttcagt tgataaaagc cggataccgc gttctgatcc tggttaaggg tgaagaagat
     1621 ttagcggttg ttcccgctgt aattctagcc cctcctggtt cactggtcct ctatggccag
     1681 cccggggagg gtgtggtgat ttgtgaagtt gaccgcctga gagataaagt tattaagatg
     1741 aaagagatgc taatggagga ataagatgga aattgatatt aaagaaaaag ttgaaaatcc
     1801 acttctaaac cggacagaaa taagattcga ctgtctgtat caaggagaag ctactccaaa
     1861 agttttagat gttaaaaaca gactggtcgc agttttgaat gtggacaaag accttttggt
     1921 agtggataaa atgaagccca gttacggtga aggccgatcc gagggctacg ccaagttgta
     1981 cgactctccg gaccatctgt cccaggtgga aaggaaccat gttatggtca agaaccagga
     2041 gaaaaccacc ccagaaagtg aagaagaata aaacacctga ggattttaat atgaaaaagt
     2101 accagcttta cgaagttaaa gataacaaac tcgtccgtaa gaatccagag tgtgtacgat
     2161 gctcccacgg agtcttcatg gcggaccatg gagatcgtta cacctgtggt aaatgcggat
     2221 acacccaatg gaagggtaaa gaaaagaaat aggttggatt gattttgaac ttgaggtcag
     2281 gaagattcaa gggcaggatg accagcgacg cagctagtta cacttcctcc ctagaatttg
     2341 acccacgaat tttcagtgcc gatgtaaagt gcaaccgggc ccacaccacc atgctccagg
     2401 aggagggcat catctcccag gaaactgccc atgccataac agggtccctg gatgaactgg
     2461 aagaaaaagg aatcagtgcc ctagacctgg acccctcggt ggaagacatc cacatggcag
     2521 tggagaacta cgttaccagc aggattgggg agaaagcagg tttcatgcac actgccaagt
     2581 cccgtaacga ccaggtggcc accgacctca gaatatccct caaagaagag atagaagaaa
     2641 taatggggga cctgcttaat tttatacaga acacagtgga tatggctgag gagaacctgg
     2701 agacagtgat ggtgggttac acccacctgc agcatgccca gcccaccacc ttcgcccacc
     2761 accttttatc ctatgctcag gcacttaaaa gggattacca gagactggga gatgcctata
     2821 aacgggttga tctttgtccc ctgggtgcgg cggccctgac cacaactagt tttcccatca
     2881 acaggcaacg cacagcccag cttctgggat tcagccatgt cctggagaat tccctggacg
     2941 cagtgagcag ccgggacttc ataactgaaa ctgtgtcggc tctggccatt atgggggtga
     3001 atctcagccg gatctgcgag gaactcatca tctggagtac ttacgagttt ggcctggtgg
     3061 agatagggga tgaatattcc tccaccagtt ccatcatgcc ccagaagaag aaccccgatg
     3121 tggcggaggt ggcccgggcc aaggccatca gtttgcaggg agaactggta accatcctat
     3181 cggtaaccaa ggccctgccc cagagttaca accgggactt gcaagaagta acaccccacc
     3241 tttggagagc cgtggacacc gcccgggaca ccctgaacct ggtggcggag atgctggctt
     3301 cggtgaagtt caaccaccag cgaggattgg agttagccag ttccaacttc tccaccgcca
     3361 cagaattagc cgacctcatg gtccgggaga atcaactacc cttcagggta gcccatcaga
     3421 tcgtgggcag gttggttagc accgccctag aggaaggttt gaatccggac ctgattgatt
     3481 cccagttcat agacc
//
LOCUS       DAOQVW010000124         3431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_31487_length_3431_cov_3.311908, whole genome shotgun sequence.
ACCESSION   DAOQVW010000124 DAOQVW010000000
VERSION     DAOQVW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31487_length_3431_cov_3.311908"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2216)
                     /locus_tag="PKK85_03670"
     CDS             complement(<1..2216)
                     /locus_tag="PKK85_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS25146.1"
                     /translation="MKEILLTKIALQKSEEKYRTLFETITQGVVFQDVHGHITSMNPA
                     AEIILGYDCEEILNKKSDNPLWGAVKEDGTHFPREVHPYMRALKTGEKVQGVVMGIEN
                     PSKEGYTWLSINAIPLFQPGETKPYQVYTIFEDISSRKNMEKALIEKERKLSALYETL
                     PIGVSIVNKNRDVLDVNPAMEKILKLSKEEILRGKHKKRRYIKGDGSEMSIDELPSSI
                     VLHGGGEIVEDDVGVVTEDDETIWIKVIARTLPFPDWKVLILTSDITQRKNSENALKE
                     SERNYRELVHNSLVGVYKSNLKGDILFANEALTRIFGFDSVEELKEQNIKNLYKYQED
                     RTSLIHDLKECGGLADYELEMVGKNGQGINLLVSASLKDDVISGMFMDVTRGKLAEQK
                     LRESEERYRSLFNQMSEGFAVHEIIFDSKGGPIDFRFLDINPAFEKLTGLKKDEVVGK
                     LQSEVVPGDTVDWVSIYGEVALTGKHKHFDGYSDTLKRHYDVCTYSPKPHHFATIFSD
                     ITERKGSEENLKSTLGKLEKSNYDLEQFAYVASHDLQEPLRMIISFLQLLQRRYGESL
                     DEEAQEFIGFAVDGAVRMQKLINDLLKFSRLNTKPVKFREVSTEEVLEQVVFESKIFI
                     EKNDAVITHDPLPVIFADFNQMVQLFQNLVVNAIKYHGPERPQIHISANKEEDGWLFS
                     VQDNGMGIDENQKIKIFKIFQRLHGRDEYEGTGIGLAIAKRIVERHGGRIWVESDPG"
     gene            complement(2149..2571)
                     /locus_tag="PKK85_03675"
     CDS             complement(2149..2571)
                     /locus_tag="PKK85_03675"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNS25147.1"
                     /translation="MSLIEPENNHILHTLDNFNYDWEYWITPSGRLIHCSPSCERITG
                     YSSQDFKKNPQLLHEIIHPDDRPLLGEHFQKTSPDDEAISLEFRIKTREGDVRWISHT
                     CKSVFDENKNYLGWRVNERDITDQNSPAEERRKISHPF"
     gene            complement(2647..2943)
                     /locus_tag="PKK85_03680"
     CDS             complement(2647..2943)
                     /locus_tag="PKK85_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25148.1"
                     /translation="MKNSAKFTSTMDVKDKNGKLLGAVCVAPAKEIGKRDILLMDEKT
                     TQSVRSITELINMLSKKNVSFEERKQVLDFLSERLRYLEKEITLNTLKTLKDSQ"
BASE COUNT          912 a          750 c          632 g         1137 t
ORIGIN      
        1 cctggatctg actctaccca gatccttcca ccgtgtcttt ccactatcct cttggctatg
       61 gccagaccta tacctgtgcc ttcatattca tctctaccat gcaatctctg gaatatcttg
      121 aagatcttga tcttctggtt ttcatcaatt cccataccat tatcctgaac tgaaaacagc
      181 cacccatcct cttccttatt ggcggaaata tggatttggg gtctttccgg cccatgatac
      241 ttaatggcgt tgacgacaag attctggaac aactgtacca tctggttgaa atcagcaaat
      301 atcactggga ggggatcgtg ggtgattact gcatcgtttt tctcaatgaa aatttttgat
      361 tcaaaaacaa cctgttctaa cacttcttca gtgctaactt ccctgaattt cactggtttg
      421 gtgttgagac gtgaaaattt tagaagatca ttgatcaatt tttgcattcg aaccgcacca
      481 tccaccgcaa accctatgaa ttcctgggcc tcttcatcca aactttcccc gtaacgcctt
      541 tgtaaaagct gcaggaagct gataatcatg cgtaaaggtt cctgcaagtc atgagaagct
      601 acgtaggcga actgttccaa atcgtaattt gatttttcca gcttccccag tgttgattta
      661 agattttctt ccgatccttt ccgttcggtt atatcactaa aaatagttgc aaaatgatgg
      721 ggtttgggag agtaagtaca aacatcatag tgacgtttca aagtatctga gtagccatcg
      781 aagtgcttat gtttgcctgt aagagctact tccccataaa tcgaaaccca gtctacagta
      841 tctccaggca caacttcact ttggagttta ccaactacct cgtctttttt cagccctgtt
      901 agtttttcaa aggcggggtt aatatctaaa aatcggaaat caattggtcc tcccttagaa
      961 tcaaagataa tctcgtgtac tgcaaagcct tcggacatct ggttaaacaa ggaacggtaa
     1021 cgttcttcac tttctcttaa tttctgttct gccagttttc ctctagttac gtccatgaac
     1081 atccccgaga tcacgtcatc ttttagactg gcactaacta gtaagttgat gccctgacca
     1141 ttttttccaa ccatttccaa ttcataatct gctaagccac cacactcttt cagatcatgg
     1201 atcaatgaag ttctatcctc ttgatatttg tacaaatttt tgatattctg ctctttcagc
     1261 tcttcaacac tatcaaaacc aaatatcctg gttaacgcct cattggcaaa cagtatatcg
     1321 ccctttagat tgctcttata aactcccacc agggaattat gcaccaactc tcgataattt
     1381 ctttcactct ctttaagggc attttcggag ttttttcgtt gagtaatatc tgaggtgagg
     1441 atcaacactt tccaatcagg gaagggtagg gtgcgtgcta taaccttgat ccagatggtt
     1501 tcatcatctt cggtgactac gccaacatca tcttccacaa tttcaccccc accatgcagt
     1561 actatggaac tgggaagttc atctatggac atttcactcc catcaccttt aatatatctc
     1621 ctcttcttat gttttcccct gagaatttcc tctttagaaa gtttaagtat tttctccatg
     1681 gcagggttaa catctaagac gtcacgattt ttattcacaa tggaaacacc tatggggagt
     1741 gtttcgtaga gggcgcttaa ttttctctct ttttcaatga gagctttttc catattttta
     1801 cgggagctaa tatcttcaaa aatagtgtaa acctggtagg gcttagtttc acccggctgg
     1861 aataagggga tggcgtttat actcaaccaa gtgtaaccct cctttgatgg attttcaatt
     1921 cccatcacca ccccctgcac cttttccccg gttttcaagg ccctcatgta ggggtgtact
     1981 tctctgggaa aatgggttcc atcttccttc actgcccccc agagggggtt atcagatttt
     2041 ttattaagaa tttcttcaca atcatatcca agaatgattt cagccgcagg gttcatggat
     2101 gtgatgtggc catggacatc ctggaaaacc accccctggg ttatggtttc aaaaagggtg
     2161 cgatattttt cttcgctctt ctgcagggct attttggtca gtaatatctc tttcatttac
     2221 cctccatccc aggtaatttt tattttcatc aaaaacagat ttacaagtgt gtgaaatcca
     2281 gcgaacatcc ccctcccggg tttttatccg gaattccagg ctgatggctt catcatctgg
     2341 acttgttttc tggaaatgct cacccagcag tggtcgatca tcggggtgga taatttcatg
     2401 taatagttgg ggattttttt taaaatcctg cgatgaatat ccagtgatac gttcacaaga
     2461 aggagaacaa tgaattaacc taccactggg tgttatccag tattcccagt catagttaaa
     2521 gttgtctagt gtgtgaagga tatggttgtt ctctggttca attagagaca ttttatcggc
     2581 tgctagtttt ggtccagtat accctggaaa gtcctgaata agatagagtt aaactatttt
     2641 ttatcatcac tggctgtctt tcaatgtctt caaagtgtta agggttattt ctttctctaa
     2701 atatcgtaat ctttcagata agaagtctaa tacttgtttt cgttcttcaa atgaaacatt
     2761 tttcttagaa agcatgttaa ttagttcagt aatactccgg acggattgtg tggttttttc
     2821 atccatcaaa agtatgtccc gttttccaat ttccttggct ggagctacac agactgcccc
     2881 taaaagtttt ccatttttat ccttcacatc catggtactg gtaaatttgg cggaattttt
     2941 catgttaatg caccgtatat tagttatttc aacttcttaa ataaattttg tgctaaaaat
     3001 ttgcactata tggaatattc cgggtaatta aaagggttcc cagagtttag cactctggga
     3061 atccttatca tcccgtttta cggaggcggt aacgggggtg atattagaac tatattcagt
     3121 ctaattcacc taattacatc ttacttctgg aaaatatata tacatttcta tgaagattca
     3181 gcatccagtt tgagtagtaa caatttagca agaagtaatg gaagatggaa aagtatataa
     3241 gtatattctt atatacttaa gaatcatgat gaggataaac tccggggcca gctccttaat
     3301 atctgctgaa ttggaagagc tattcaaggc cttgagtaac ataaacagac tccttctggt
     3361 ctattccctg gcatctggag aagtggagcg gatcagcgta accgagatgt ctaaactaat
     3421 gggtttaacc c
//
LOCUS       DAOQVW010000125         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_31603_length_3423_cov_3.330463, whole genome shotgun sequence.
ACCESSION   DAOQVW010000125 DAOQVW010000000
VERSION     DAOQVW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31603_length_3423_cov_3.330463"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKK85_03685"
     CDS             complement(<1..293)
                     /locus_tag="PKK85_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS25149.1"
                     /translation="MSYYHDYRMEKIFQALEQPKTIESLQLSESFITDLFLKIVAGYG
                     TIKTSTINEITGIHWDILEKILPELEKSGLCATVGGGFLFSSVEYSITPKGRE"
     gene            complement(313..993)
                     /locus_tag="PKK85_03690"
     CDS             complement(313..993)
                     /locus_tag="PKK85_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02253 family HAD-type hydrolase"
                     /protein_id="HNS25150.1"
                     /translation="MVKAVFFDIDDTLYDTSGFAKLARKAALSAMIDAGLPLSNPEAY
                     SLLRSIIKEKGSNYNKHFNVLTQRVFGEENPYLIALGMITYHNVKFALLRLFPDTMST
                     LIHVKKQGYHLGVISNGLVIKQWEKLIRLDLHHFFDAVVTSQEAGCEKPEPEIFNLAL
                     RRIGCRADKSIMIGNQFSEDILGALNVGMSAILVNSRLTAEEEDYLRKNNLEIDVAPH
                     IGLIREIL"
     gene            complement(1078..1737)
                     /locus_tag="PKK85_03695"
     CDS             complement(1078..1737)
                     /locus_tag="PKK85_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNS25151.1"
                     /translation="MVCLLRNIIQDEAKKFTEAVNQELPEGMELEFEGFFERGFFVTK
                     KRYALIQEGKIVVKGLELVRRDWAPVARKTQEKVLRAILEDGSPKKATKIVRDVIQDI
                     KNGDLDLEDLVINTQITRNPESYQQKAPHVMAAKKAMERGRKVVRGSIMRYVVVKGKT
                     PISQRAEPVEDVDISDYDPVYYIENQVLPAISRIIEAIGYSPSEVMHQERQSSLDAFF
                     N"
     gene            complement(1718..2098)
                     /locus_tag="PKK85_03700"
     CDS             complement(1718..2098)
                     /locus_tag="PKK85_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HNS25152.1"
                     /translation="MAIWQGKSVRKLTGGRSKSSRNKRKIEFGRDPAETKIGERKRKT
                     IRTKGGGKKLRLTNDQKINVVDPKNNKMQIAEILGVIENHANDHFVRRNIITKGAVVE
                     TNLGQVKITSRPGQDGVVNGVLIT"
     gene            2384..3394
                     /gene="hypE"
                     /locus_tag="PKK85_03705"
     CDS             2384..3394
                     /gene="hypE"
                     /locus_tag="PKK85_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNS25153.1"
                     /translation="MKIGMSHGAGGEMMQGLISDIILKNISNKQVEDGVGLQDLDDGA
                     TIPLGDYEVVVSTDSHTIDPLFFPGGDIGRISMAGTVNDVSVMGARPLAITNAMVISE
                     GFQKDELERIIQSMDAVSQETGVAIVTGDTKVMEHDQLDKMIICTTGIGIAPRGCITR
                     DSSLEVGDKIILSGSVGDHGIALMSYREGFGFETDLKSDVAPVWGMVEAALDVGGISA
                     MKDPTRGGISNALNELASKSGVGMILDEEKIPVKREVHAASEMLGIDPFEVANEGKVI
                     MGVKPDQADEVLTAIRKQRYGKNAQIIGEVTLNGPVILETLLGGKRILETPIADPVPR
                     VC"
BASE COUNT          904 a          698 c          753 g         1068 t
ORIGIN      
        1 ttttctcgtc ctttgggggt tatactgtat tccacgcttg aaaatagaaa tccgccaccc
       61 accgtggcgc aaagaccact tttttccagt tctggtaaaa tcttttccaa tatatcccag
      121 tgtatacccg taatttcgtt aatggtgctg gtttttatgg ttccgtaacc agccacaatt
      181 ttcaaaaaga gatctgtaat aaaagattcg gaaagctgta agctttctat ggtttttggc
      241 tgttccagtg cctgaaaaat tttttccatt cgatagtcat gataataact cattaaaacc
      301 actaccaacc ttttacagaa tttctcgaat taaccctatg tgaggtgcta cgtcaatttc
      361 taggttgttc tttcttaaat aatcttcttc ttctgcagtg agtctggaat taaccagtat
      421 ggctgacatt cccacgttga gggcacctaa aatatcctca gaaaattggt tcccgatcat
      481 tatagattta tcagccctgc agcctattct tctcagggcc aggttaaaaa tctcgggttc
      541 aggtttttca catcctgctt cctgggaggt gactactgca tcgaaaaaat ggtgcaaatc
      601 cagcctgata agcttttccc actgtttaat taccaggccg ttagatatca ctcccaagtg
      661 atacccttgt ttttttacgt ggatgagtgt ggacatggtg tccgggaata gccgtagtaa
      721 tgcaaatttg acgttatgat aggttatcat tcccagagca attaagtaag gattttcctc
      781 cccaaaaact cgttgagtaa gtacgttgaa atgtttgttg taattggagc ctttttcctt
      841 gattatggat cttaaaagag aataagcttc cgggttagac aagggcagtc ctgcgtcaat
      901 catggcagaa agagctgcct ttcgagctaa cttggcaaaa ccagaagtgt cgtatagtgt
      961 gtcgtcaata tcgaaaaaaa ctgctttcac catatttgta gctccttaaa cattctggat
     1021 agtatctatt gtgtaatctt atccattata gatttattaa tgtataagaa tgaattttca
     1081 gttgaagaag gcatctaagc tactctgtct ttcttgatgc attacctctg aaggagagta
     1141 accgatggct tcaataattc gggatattgc tgggagaacc tgattttcaa tgtaataaac
     1201 gggatcgtag tcacttatat ccacatcttc tactggctcg gcgcgctgac ttatgggagt
     1261 tttccccttc actacgacgt aacgcattat tgagcctcta acaacttttc tacccctttc
     1321 catggccttt ttcgccgcca ttacatgagg tgctttttgt tggtagcttt ctgggtttcg
     1381 ggtgatctgg gtgttgatga ccaggtcttc cagatccaga tctccattct taatatcctg
     1441 gataacatct cgcactattt ttgttgcctt tttgggtgat ccgtcttcca ggatagctcg
     1501 caataccttt tcttgcgttt tcctggcaac tggcgcccaa tctcggcgta ccagctccag
     1561 gcctttcacc acaatttttc cctcttggat gagggcgtat ctttttttgg taacgaagaa
     1621 gcctctttcg aagaaccctt cgaattccag ttccatccct tccgggagtt cctggttaac
     1681 agcttcggta aacttcttcg cctcatcttg gataatatta cgtaataagc acaccattaa
     1741 ccacgccgtc ctgtcctggt cgggaggtaa ttttaacctg gcctaaatta gtctccacta
     1801 cagccccttt agtgatgatg ttacgtcgca caaagtgatc gttggcatgg ttttcaataa
     1861 cgcccagaat ctctgcaatc tgcattttgt tgtttttagg atccacaacg ttgatcttct
     1921 ggtcgttggt gagcctcagt ttctttccac cacccttggt acgtatggtt tttcttttac
     1981 gttctccgat tttggtctca gcagggtcgc gaccaaattc tatttttctc ttattccggc
     2041 tgctcttgga gcgtccgccg gttaattttc taacagattt tccttgccat attgccatta
     2101 tttcacctta ttaacttaat ttttacaaag tattctgcct ttgatttttt taaaaaaagc
     2161 agtaaggaaa taaacagtat ttctacagag tttatgtttc gagactttat aatacttttc
     2221 tattgtgact atatggtggg agcaaggatt taaatatttc aaactccaac ttcagcaaca
     2281 gagaaaacat ccccttattt tctcgtcaac taatttaagg gaatttgaag tattaaaaaa
     2341 taattttaga ctaatttatc aaattattag tactggtgaa gtcatgaaaa ttggaatgtc
     2401 tcatggggcc ggcggagaga tgatgcaggg cctgatttcg gatataatac ttaaaaatat
     2461 aagtaacaag caagttgaag atggggtggg actacaggat cttgatgatg gtgccactat
     2521 acctctggga gactatgagg tggtggttag cactgatagt cacaccattg accctttgtt
     2581 ctttccaggc ggagatatag gtcgtatatc aatggctggt actgttaacg atgtttcggt
     2641 catgggagcc cggcccctgg ccattaccaa tgctatggta atcagtgagg gatttcaaaa
     2701 agatgaactg gagcgtatta tacagtccat ggatgcggtc agccaggaaa caggggtggc
     2761 catcgtaact ggagacacca aggtgatgga gcatgatcag ctggacaaaa tgataatatg
     2821 taccactggg attggaattg cccctcgggg atgtatcact agggattcca gcctggaggt
     2881 gggagataag atcatactca gcggtagcgt aggtgaccat ggcatcgcct tgatgtccta
     2941 ccgggaagga tttggttttg aaactgattt aaaatccgat gtagctccgg tatggggcat
     3001 ggtagaagct gctctagatg taggtggtat cagtgcaatg aaggacccca cccggggggg
     3061 tatatccaat gctctaaacg agttagctag caaatcaggg gtgggaatga ttctggatga
     3121 ggaaaaaata ccggtaaagc gtgaagttca tgctgcctca gagatgctgg gtatagatcc
     3181 ctttgaagtg gccaatgagg gtaaggttat aatgggcgtg aagccagatc aggcagatga
     3241 ggtacttact gctatccgta aacaaagata cggtaagaat gcccaaataa ttggtgaggt
     3301 aacattaaac ggtccagtta tactggaaac ccttttagga ggtaaaagga tactggaaac
     3361 ccctattgcc gaccctgttc ccagggtatg ttaaagaaaa tggtgtgttt atggaaaaag
     3421 aga
//
LOCUS       DAOQVW010000126         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_31956_length_3396_cov_4.472014, whole genome shotgun sequence.
ACCESSION   DAOQVW010000126 DAOQVW010000000
VERSION     DAOQVW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31956_length_3396_cov_4.472014"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..669
                     /locus_tag="PKK85_03710"
     CDS             400..669
                     /locus_tag="PKK85_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25154.1"
                     /translation="MKKDFKRLDGLLHEIRARCTGRDDRQLLKAILQEHLITLENEDL
                     LEELDLDLPKIEIELEDIDLELDFKDIELEPFDPCPKRSKKDDQG"
     gene            656..1513
                     /locus_tag="PKK85_03715"
     CDS             656..1513
                     /locus_tag="PKK85_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNS25155.1"
                     /translation="MIRDEDLEDYLTRLEVLGRSQVTIKNYRAFLEPFIRYTRDHDLT
                     PENCRELIEAYQLHLKRDHHYNNESLKTATTVIKLFLTKLGYPCQGVELPKTRKRLPK
                     YLSRDEVEKLLQAPGGVYETRDRAVLRLLYTSGLRVSELVALNIEDVDFNEGTVKVND
                     GKGGRDRVTYTDRETLQLLKDMIYKRTRKGREDKGPALFTTRSGDRISTRSIQRIVKK
                     TSQEAGLEKRVTPHIIRHSFAANSLEEGFNIKEIQQLLGHSSLSSTEIYTQIKPKDLE
                     KKYKKLKWD"
     gene            1623..1934
                     /locus_tag="PKK85_03720"
     CDS             1623..1934
                     /locus_tag="PKK85_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25156.1"
                     /translation="MRDERLKHLEHETINKMELIHEDRENDQIVALINEYSYTFRPWQ
                     WELIQEENQKPEGEGRFYIHNYYTVSPDEEGELQLWLNKRVKCKGYDLGGCFISHLGA
                     A"
     gene            1946..2185
                     /locus_tag="PKK85_03725"
     CDS             1946..2185
                     /locus_tag="PKK85_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25157.1"
                     /translation="MMVERPILRIIVSDKKELLDPKSLEGYQPGDTVALIYGPDLEKL
                     VNDLKELLDDYQELYKKYDEAREVIKKHIEKQGGG"
     gene            2949..>3396
                     /locus_tag="PKK85_03730"
     CDS             2949..>3396
                     /locus_tag="PKK85_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25158.1"
                     /translation="MTDNRKKIKVDGEVYSNFRDHIKRTKGKIRGEMTPALEEALELY
                     IKIHDNPDLKRRVYEILPEGHGSHPQNQKFFNEFEERFEGYHLVGSGELNNWIKTYTG
                     AYSLTALKKWRNRLKDTDRIIYINNNRWRLPGNDTGDQATLTPAPHK"
BASE COUNT         1150 a          611 c          772 g          863 t
ORIGIN      
        1 cagctataaa atctaataga cattatattg gttatgatat caaagaagag tatgtaaaac
       61 tctctaaaga tcgaatttca agctataaag atcaatcgaa attaatctga ctttatatct
      121 tcttttgtgt tttcagcaca tcccgaagca gaacctacca gtaaaaccaa tactgtaagt
      181 aaaactattt taaactccat tttaacccct tattcttatt gttactcctt aatatattat
      241 tctattctct tttggtatag ggtggccttg acttttaggt tactttttcc ctccttttta
      301 tactgagtta cactttataa taataaaacg acatatttat atgtcctaaa aataagtata
      361 atatattcac atgaacatta aaaggggtgt ttaatcaaca tgaaaaaaga ttttaaaaga
      421 ttagacgggc tcctccatga aataagggcc cggtgcaccg gcagggatga ccggcagctc
      481 cttaaggcca tactccagga gcacctaata accttagaga atgaggatct cctggaggag
      541 ctagacttgg atctccctaa aatagagata gaacttgaag acatagacct ggagctggat
      601 tttaaagaca ttgaactgga gccttttgat ccgtgcccta aaaggagtaa gaaggatgat
      661 cagggatgag gacttggagg actacctaac caggttagaa gtattaggcc gctcccaggt
      721 aaccattaaa aactatcggg cctttctgga gcctttcata aggtacaccc gggatcatga
      781 cttaacccct gagaactgcc gggagctgat cgaagcttac cagctccacc ttaaacggga
      841 tcatcattat aataatgaga gcctgaaaac ggccactact gtaataaaac ttttcttaac
      901 taagttaggt tatccctgcc agggcgtgga gctccctaaa accaggaaaa gactccctaa
      961 gtacctctcc agggatgagg tggagaaact cctccaggcc ccgggtggtg tatatgaaac
     1021 ccgggatagg gccgttttaa ggttacttta tacctcaggt ttaagggtta gtgaattagt
     1081 ggccctaaac attgaggacg tggattttaa tgagggcacc gttaaggtaa atgatggaaa
     1141 aggaggacgg gaccgggtga cctatacgga ccgggaaacc ctccagctcc tcaaggacat
     1201 gatttataaa agaacccgaa aaggccggga ggataaaggc ccggccctat tcactactag
     1261 atcaggggat cgaatatcca cccgctcaat tcaaagaata gtaaagaaaa cctcccagga
     1321 ggccggcctg gagaaaaggg taacccctca cataattaga catagttttg cagctaatag
     1381 cctggaggag ggctttaata tcaaggagat ccagcaactt ttagggcata gtagcctttc
     1441 aagtactgaa atatatactc aaatcaagcc taaagacttg gagaaaaagt ataaaaaatt
     1501 aaaatgggat taagtgggag tagtgagaaa cggtaaaacc gaccctcacc actagggata
     1561 ttactctttt caagtactta aacatggtgg ttaaaccaaa aaatgaatta aaggaggtaa
     1621 aaatgagaga tgaaaggctt aaacacttag aacatgaaac cataaataaa atggagctaa
     1681 tacatgagga ccgggagaac gatcagatag tggccctaat caatgagtac agttacacct
     1741 tcaggccctg gcaatgggaa ttaatccagg aggaaaacca aaaaccagag ggagagggcc
     1801 gtttttacat tcataattat tacacggtaa gccccgatga ggagggagag ctccagttat
     1861 ggcttaataa acgagtgaaa tgtaaaggct atgacttggg agggtgcttt atatcccact
     1921 tgggagctgc ttaaaagggg gttttatgat ggtagagagg cctattttac gtattatagt
     1981 atctgataaa aaggaactat tagatcctaa aagcctggaa ggataccagc caggggatac
     2041 ggtggcctta atatacggcc ctgacttgga aaagttagta aatgatttaa aggagctttt
     2101 agatgactac caggaactat ataagaaata tgatgaggcc cgggaggtca taaaaaaaca
     2161 catagagaaa caaggaggag gttagggagc tcccaggtaa aaggggatcc ctgccggtca
     2221 tgagaacacc ctatttttat agggtggggg gtgtaatccc tgccgctcca ggagagggtt
     2281 tttcaaagaa aaggagcgtt taaggggctc ccagggatta ggttactatc caggtaaaag
     2341 taaattatat gcaccttata ggccacccct ggagatggtt taaataggcc cccgggcccg
     2401 ggagggtgga ggataaaaca taaacattta ttatatgcct aatatagata gatggtttaa
     2461 aagaactcaa tagaaaatta gcaaaaagga aaggacccct gggatggggg ccctgttgta
     2521 ataatggtta tgggagccta tgctatttaa atctttatgg tataaagtga ccgtgacctg
     2581 gagaccctct tttttctatg agtttttaca caccctgaaa ttataagatt tttaggggtg
     2641 gctaccatga ccccctaact ccctcaggtg tatcctaagg gtagatcatg gagggtgtgg
     2701 taagaatata ttaagctccg tttaataaac ggggtaaata tatggattta cttgaggaaa
     2761 aagaaaagga taatagttta aatctctcta taagtgttaa aaataatata atataatata
     2821 atataataaa tataataata atatatataa taataatact tataggtaag agataagtcc
     2881 ccttatctaa tagataagtc cccttttcta ggcagctcct caagttgaaa ttagggggct
     2941 tttgagggat gactgataat agaaagaaaa taaaggtgga tggagaggta tattcaaatt
     3001 ttagggatca tataaagcgt actaaaggaa aaataagagg agagatgacc ccggccctgg
     3061 aggaggccct ggagctttat attaaaattc atgataaccc ggatctcaag aggagggtat
     3121 atgaaatcct cccagaggga cacgggagcc acccccaaaa ccagaaattt tttaatgaat
     3181 ttgaggagcg ttttgagggc tatcacttgg taggatctgg agagctgaat aactggatca
     3241 aaacatatac cggggcttat agcttaacgg cccttaaaaa atggagaaac cgtttaaaag
     3301 atactgatag aataatttat attaataata accggtggag gctcccaggt aatgacactg
     3361 gggatcaggc caccctaacc cctgcccctc ataaaa
//
LOCUS       DAOQVW010000127         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_32067_length_3387_cov_3.321729, whole genome shotgun sequence.
ACCESSION   DAOQVW010000127 DAOQVW010000000
VERSION     DAOQVW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32067_length_3387_cov_3.321729"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKK85_03735"
     CDS             complement(<1..399)
                     /locus_tag="PKK85_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNS25159.1"
                     /translation="METDLYKIDVCLARPFLKWAGGKAQLLGEFHKRIPQSIMGRGII
                     ERYVEPFLGGGALFFYLKRNFQVKESFLFDINQDLVVGYRVLKNDPSHLITELSNLED
                     KYRNKSDEERETFFYNIRKSFNQQRADFDYE"
     gene            complement(472..1254)
                     /gene="dph5"
                     /locus_tag="PKK85_03740"
     CDS             complement(472..1254)
                     /gene="dph5"
                     /locus_tag="PKK85_03740"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HNS25160.1"
                     /translation="MLYLVGLGLYDEKDISLKGLEALKRADVVYAEFYTARLFGGDLH
                     SLEELTGAEIKVLRREEVEEENRPLQDAVTMDVAFLTAGDPLVATTHTDLLIEARKSG
                     IDVQVVHSSSILSAAPGLAGLQAYKFGKVTTIPRPEENYFPHSPYQVVASNLKMDLHT
                     LALLDIQAHHDYYMTANQGLEYLLKVAQDRGDQLLNEDSLAVVVARAGDTKPLVRADQ
                     MGKLVNEDFGGPLHCLIIPGKLHFLEAEALVVLGGAPGEILE"
     gene            1382..2395
                     /locus_tag="PKK85_03745"
     CDS             1382..2395
                     /locus_tag="PKK85_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HNS25161.1"
                     /translation="MIGLKVPKKEANQLRLLLLEHKILDRNFKIKRSLDYVFIPLLER
                     PGMDLMKNESLQVVDTEFEEAKRSPRSMEEYLEGQIPAEKMEDLKKSFDIIGEVVILE
                     IPEELEGEKKAIAEAALKFTKRRSVYRKGSEVKGTTRTRELEHLAGEDLSETTHQEFG
                     TRIMLDVKNVYFSPRLATERRIVTDQVKEGEVVLDMFTGVGPFALSIARNHQVKVYAT
                     DINPMAIFYLKRNLEINKLQERVVPLGGDVKEILEGLDLKFDRIIMNLPGSAREFLPV
                     ALEHLKSGGVVHYYQFSRDMDDPIQSIKNSAKDRQVEILDKRKVKSTRPGEWHVAVDA
                     RIC"
     gene            2452..2877
                     /locus_tag="PKK85_03750"
     CDS             2452..2877
                     /locus_tag="PKK85_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25162.1"
                     /translation="MMNILPEVLEIEYSFPKDGGHNTLKLVEGRLFFFSEAHSRLSEK
                     NEYLTMVSAPERDDWILFWKALNQAGVWDWEEDYRPEPGLTLEADTWLVKIHRGEQTM
                     QTRSWFVEPLTRNEFFLALKHLAGVDITLPYQYLSRKLR"
     gene            complement(2890..>3387)
                     /locus_tag="PKK85_03755"
     CDS             complement(2890..>3387)
                     /locus_tag="PKK85_03755"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNS25163.1"
                     /translation="TALGVVRAAHEEGKNVKVICDETRPVLQGARLSVWEMQEEGIPV
                     KLIVDGAAGRLMQEGEVDKVIIGADRVAQGGVANKIGSLMVALAAHRFGVPFYVAAPK
                     STFDHENSIYEIEIEERNPEEVLNFAGCRVAPLLTEVRNPSFDVVPADLITGIITEKG
                     IMKPF"
BASE COUNT          851 a          848 c          836 g          852 t
ORIGIN      
        1 ttcatagtca aagtcagccc tttgctgatt gaaactcttt ctaatgttgt aaaaaaatgt
       61 ttcacgttct tcatcacttt tattcctgta tttatcctct aaattactta attcagtgat
      121 caggtgactt ggatcatttt ttaggaccct gtatcccact actaggtctt ggtttatgtc
      181 gaaaaggaat gactccttaa cttggaagtt tcttttcaga taaaaaaaca gggcgccccc
      241 accaaggaag ggctccacat atctttcaat gatcccccta cccattatag attgcgggat
      301 ccttttatgg aactctccta ataattgggc tttacctcca gcccacttga gaaaaggccg
      361 ggccaaacag acatctattt tatataaatc agtttccatg gcatcatccg actagtatag
      421 aattttcccc ttcattcctg agggttttct tttatttacg tgaagatcat tttattcaag
      481 gatctcccct ggagccccac ccaacaccac cagtgcctcg gcctccagga agtgcagctt
      541 ccctgggatg atcaggcagt gcaatggacc cccaaagtcc tcattaacca gttttcccat
      601 ctgatctgcc ctgaccaggg gtttagtgtc acctgcccgg gccaccacca ctgccaggct
      661 atcttcattt aataactggt ccccacggtc ctgggctacc tttaaaaggt actccagtcc
      721 ctggttggca gtcatgtagt agtcatggtg ggcctggatg tccaggaggg ccagggtgtg
      781 caggtccatc ttcagattac tggctacgac ttggtagggg gagtggggga agtagttctc
      841 ctcgggtcgg ggaatggtgg tgaccttacc aaacttgtag gcctgtaaac cagccaaacc
      901 cggagcagcc gagagtatgg aggaggaatg aaccacctgg acatctatac ctgacttacg
      961 ggcttcaatt aaaagatccg tgtgggtggt ggccaccagg ggatcgcctg ctgttaaaaa
     1021 agccacatcc atagtcactg catcctggag gggcctattt tcttcttcca cctcttcccg
     1081 tcggagtacc ttaatctcag ctccagttaa ctcttctagg ctgtgcaggt caccgccgaa
     1141 gagacgggcc gtgtaaaact cggcgtaaac cacatctgcc cttttaaggg cctccaaacc
     1201 ctttagggat atgtcttttt catcgtaaag tcccagtcca accaggtaaa gcatatttcc
     1261 accttcaatt cctagttatg ggctgagggg tttataaaat tttattttaa ggtgataaca
     1321 taattatgtt aggcataact tgccccgtag tatttgttta caaaaaaaaa tgaggaaacc
     1381 catgatcgga ctcaaggtcc ctaaaaaaga ggccaaccag ttacgcttgc ttcttttgga
     1441 acataaaatt ttggaccgga atttcaaaat taaacgctcc ctggattacg ttttcatacc
     1501 cctcctggaa aggcccggga tggatttaat gaagaatgaa agcctccaag tagtggacac
     1561 cgaatttgag gaggctaaac gaagtccccg cagtatggag gagtacctgg aaggccagat
     1621 ccccgccgag aagatggagg accttaagaa gtcctttgac atcatcggcg aggtggtgat
     1681 actggagata cccgaggaac tggagggtga gaaaaaggcc atcgccgagg ctgccttgaa
     1741 gttcaccaaa cgccggtcag tgtatcggaa ggggagtgag gtgaagggaa ccacccggac
     1801 ccgggaactg gagcacctcg caggggagga tctctccgag accacacacc aggagttcgg
     1861 aacacgtatc atgctggacg tgaagaacgt ctacttcagt cccaggctgg ccaccgagag
     1921 gaggatcgta actgaccagg tgaaggaggg ggaagtggta ctggacatgt tcaccggagt
     1981 gggtcccttc gccctctcca tcgcacgtaa ccaccaggta aaggtatacg ccacggatat
     2041 caaccccatg gccatctttt accttaaaag aaacttggag ataaataaat tacaggaacg
     2101 cgtagttccc ctggggggtg atgtgaagga gattctggag gggctggatc ttaaatttga
     2161 ccggataatt atgaacctgc ctggctcggc ccgggagttt ttgccagtag ccctggagca
     2221 cctgaaatca gggggtgtgg tgcattacta ccagttcagc cgggatatgg atgatcccat
     2281 ccagagtatc aaaaattcgg caaaagaccg gcaggtggag atacttgata agagaaaggt
     2341 gaagtccacc aggcccgggg agtggcatgt ggcagtggat gcccgtatat gctaaaggtt
     2401 tacttaatca gaaagtgttg agcacatcat aaaattatag gaatgagtta gatgatgaat
     2461 attctaccgg aagtactgga aattgagtat tcattcccaa aggatggggg tcataacacc
     2521 cttaaactgg tggaaggtcg gcttttcttt ttcagtgaag cccattcgcg actttccgag
     2581 aagaatgaat atttaaccat ggtatctgct ccggagaggg atgattggat actgttctgg
     2641 aaggccctga accaggctgg agtctgggac tgggaggagg actatcgtcc cgagccagga
     2701 ttaaccctgg aagctgatac ctggctggtg aaaattcatc ggggagagca gaccatgcag
     2761 accagatcct ggttcgtgga acccctcacc cgcaacgaat ttttcctggc cctgaaacac
     2821 ctagccgggg tggacatcac cctgccctac cagtacctgt cccggaagct tagataatga
     2881 atgatcttat tagaagggtt tcataatccc cttctcggtg atgatgcctg tgatgaggtc
     2941 tgcaggcacc acatcaaagg agggattcct cacctcagtt aacagtggtg caacccggca
     3001 ccctgcgaag tttagaactt cttcaggatt tctttcctct atttctatct cgtagatgga
     3061 gttttcatgg tcgaaggtgc tttttggtgc cgccacatag aatgggaccc cgaatctgtg
     3121 ggcagccagg gccaccatca gggatccgat cttgttggcc acccctccct gggccacccg
     3181 gtcggctccg attatcacct tatccacctc tccctcctgc attaatcttc ccgccgcccc
     3241 gtcgactatg agtttcacgg gtatgccctc ttcttgcatc tcccacacac tcaaacgtgc
     3301 cccctgtagt acgggccttg tttcgtcaca gatcaccttt acgttcttac cctcctcatg
     3361 ggctgcgcgc accaccccca gggcggt
//
LOCUS       DAOQVW010000128         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_32093_length_3385_cov_2.981081, whole genome shotgun sequence.
ACCESSION   DAOQVW010000128 DAOQVW010000000
VERSION     DAOQVW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32093_length_3385_cov_2.981081"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..535)
                     /locus_tag="PKK85_03760"
     CDS             complement(95..535)
                     /locus_tag="PKK85_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNS25164.1"
                     /translation="MTRRSVALAACSGMSPGGLVSRVACEDVRVETERVVSICMGATS
                     ADREGFRGLIKKHPIIAVNGCQGECVNRILAQRGVKPVMTIKVQEELNQEGLEATDVS
                     RLDEMGERCVECIKMKIKESLEELRNRENHDGKRDRSSGSDQNL"
     gene            complement(585..929)
                     /locus_tag="PKK85_03765"
     CDS             complement(585..929)
                     /locus_tag="PKK85_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNS25165.1"
                     /translation="MKCNDDKCPPEELLRINHLVAGLPSDEGLYQDVELLKALSDITR
                     LKIVHLLREGELCVCEIMHALDKPQSTVSHHLNLLKNTGLVEGRKEGIWTRYQLTNPR
                     ILECMEDLTKAR"
     gene            complement(942..1670)
                     /locus_tag="PKK85_03770"
     CDS             complement(942..1670)
                     /locus_tag="PKK85_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS25166.1"
                     /translation="MDEKKTKTDLKERRRGIAFNQGNCTCCDDEKMNYTSLELKKLPE
                     EAVLELGCVDSTVLAELREGEVVLAIGAGLDVFLASHLVGGTGRVISLDSDQQLVKMA
                     QEGGYHNLEFIRGAVENIPLDDDGVDVVISNCAINQATQKQKALEEISRILKPRGRLM
                     ALDLVTEGELDITAGLECLDGALQEQEYLNTIFLAGFEMVEILTEADYQEPHLDPRWT
                     AKIKCVGVKALKGCGACPGTTCSL"
     gene            complement(1795..2676)
                     /gene="argB"
                     /locus_tag="PKK85_03775"
     CDS             complement(1795..2676)
                     /gene="argB"
                     /locus_tag="PKK85_03775"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNS25167.1"
                     /translation="METVNILIEALPYIKKFHKQKIMIKYGGHAMIDNAAKSSTARDT
                     VLLKYVGMQPMVVHGGGPEISRSMSKIGKEPRFIGGLRVTDQETMDIVTMVLVGKINT
                     EIVAHIGLHGGKGVGISGKDNLLLKARKRSPQIVVDQETGDEILVDLGLVGEIESINP
                     ELLDVLTENDYIPVISPIGVDEHAKTLNLNADTVAGEVAAQVGAEKLIILTDVPGILT
                     DPTDPGSLIRKASIREVQDLIDQGIVKDGMLPKVATCVNALEKGVKSAHIIDGRLQHS
                     ILLEIFTKKGIGTMITL"
     gene            2858..3097
                     /locus_tag="PKK85_03780"
     CDS             2858..3097
                     /locus_tag="PKK85_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25168.1"
                     /translation="MYEAEQTEVEILKDLREHYPGVLEVVYESESTYCVYADDDTLWR
                     MYDELANFFQTVEFNAGDQERRYLRIVLKGDLDNI"
     gene            complement(3124..>3385)
                     /locus_tag="PKK85_03785"
     CDS             complement(3124..>3385)
                     /locus_tag="PKK85_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNS25169.1"
                     /translation="HLIKTPAYGVYHVTNSDHCSWYEYAQLIHQMKGVEVELQPVTTE
                     EFGSPAPRPKYSVLDNYHWRLQGFPPLRSYKEALKDYLDLLK"
BASE COUNT          702 a          972 c          837 g          874 t
ORIGIN      
        1 ggcggttgtt ttgcccgcac cgttggcacc taagaatccg aatatctctc ccttcctgac
       61 ttggaaatcc accccatcca cggcccggat ctggtcatag gttttggtca gaccctgaac
      121 ttctatcacg ctttccatca tggttctccc ggttcctcaa ctcctcgagg gactccttga
      181 ttttcatttt aatacactcc acgcaccttt cacccatctc atccaggcgg gatacatcgg
      241 tggcttccag gccctcctgg ttcaactcct cctggacttt gatggtcata actggcttca
      301 cacccctctg ggccaggatc ctgttgacac actccccctg gcagccgttg actgcgatga
      361 tggggtgctt cttgatgagc ccccggaacc cctcccggtc cgcgctggtg gctcccatgc
      421 agatggatac caccctctcg gtttccaccc ggacatcctc acaggcaacc cggctcacca
      481 gccccccagg gctcatgcca ctgcatgcag ccagggctac actcctcctt gtcatctaca
      541 catcctccac acatcccatt aagaggggga ttagaatggc cacttcatct ggccttggtt
      601 aaatcctcca tgcattccag tatccggggg ttggtcagct ggtaacgtgt ccagatacct
      661 tccttcctcc cctccaccaa tcccgtgttt ttaagcaggt tcaggtggtg tgataccgtg
      721 gactggggct tgtccagggc gtgcatgatc tcacagacac agagctctcc ctccctgagg
      781 aggtggacga tcttcaaccg ggtgatatcg gaaagtgcct tcaagagttc cacatcctgg
      841 tagaggccct catcagatgg taggccagct accaggtggt ttatcctgag caactcttca
      901 ggggggcatt tatcatcgtt acacttcata tacatcccat cctataggct gcaagtggtt
      961 cctgggcagg ccccgcagcc cttgagtgcc ttaaccccca cgcatttgat cttagcagtc
     1021 caccttggat ccaggtgggg ttcctgatag tcagcctcag taaggatctc caccatctca
     1081 aatcctgcta agaagatggt gttaaggtac tcctgttcct ggagggctcc gtcaaggcac
     1141 tccagcccgg ctgtgatgtc tagctctccc tcggtaacca ggtccagggc cattagtctg
     1201 cccctgggtt taagtatcct tgaaatctcc tctaaggctt tctgtttctg ggtggcctgg
     1261 ttgatggcgc agttgctgat cacaacgtcc acaccatcat catccagggg aatgttttcc
     1321 acagcacccc tgatgaattc caggttatgg tagccaccct cctgggccat ttttaccagt
     1381 tgctggtcac tgtccaggct gattacccgg ccagtgcccc ccaccaggtg ggaggctagg
     1441 aaaacatcca aaccagctcc tatggccagt accacttccc cctcccttaa ctctgccagg
     1501 acagtggaat ccacgcatcc cagttccagc accgcttctt ctggtaattt ttttaattcc
     1561 agggaggtgt agttcatctt ttcatcatca cagcaggtgc agttgccctg gttgaaggcg
     1621 atccccctgc gacgttcctt cagatctgtt ttcgttttct tttcatccat accaattaca
     1681 cccatatatc cagatatatg gatgaatggg tggggatggt atataaaatc aggggtctat
     1741 tttgtgaaaa aaaagttgga accttcctgg tgtagtacca tcccccacac caactcagag
     1801 ggtgatcatg gtcccgatac ccttcttggt gaatatctca aggagtatag agtgttgcag
     1861 ccggccatca atgatgtggg ctgatttaac gcccttctca agggcgttga cacaggttgc
     1921 caccttaggc agcatcccat ccttaactat gccctggtca atgaggtcct ggacctccct
     1981 gatactcgcc ttcctgatga gactaccagg atcagtgggg tcggtgagga tgccgggcac
     2041 atctgtgagg atgatgagct tctccgcccc cacctgggca gccacctccc cggccacggt
     2101 atcggcgttg aggttcaaag tcttggcatg ctcatccacc ccgatggggc tgatgaccgg
     2161 gatgtaatcg ttctcggtta agacgtccag gagttcgggg tttatggatt ctatctcccc
     2221 caccaatccc aggtccacca ggatttcgtc cccggtttcc tggtccacca caatctgggg
     2281 tgagcgtttc ctggccttca agagcaggtt atccttgcca gagattccca cgcccttgcc
     2341 gccgtggagg cctatgtggg ccacgatttc ggtgttgatc ttgcccacca ggaccatggt
     2401 cactatgtcc atggtctcct ggtccgtgac ccgcaggcca cctatgaacc ggggctcctt
     2461 accgatcttg ctcatggagc gggatatctc cggcccgcca ccatgcacta ccatgggctg
     2521 catacccacg tacttcaaga gcaccgtgtc ccgggcggtg ctgctcttgg ctgcgttgtc
     2581 gatcatggcg tggcctccgt acttgatcat gatcttctgc ttatggaact ttttgatgta
     2641 aggcagggcc tcgattagga tgttaacggt ttccatggtg cgcaccatct tttcaggtta
     2701 tattaacgaa tctagggcta aaatatcggt tgcgggtaat aaattcatac ttctagttgc
     2761 ttttttcccc taatacctat attaattgct gctaataaaa ataatggtat tgattcttat
     2821 aaaaaaaaat ctgtacaaga atgggggggg ttaacttttg tacgaagcag agcaaacaga
     2881 ggtagaaatc ttaaaggatc tgagggagca ctaccccggg gtcctggagg tggtatacga
     2941 atctgaatcc acctactgcg tctacgccga tgatgacacc ctgtggagga tgtacgatga
     3001 actggccaac ttcttccaga ccgtggagtt caacgccggg gaccaggaac gccgctacct
     3061 gcggatcgtt ttaaaggggg acctggacaa catctaaaaa cgtagtgcac catgggggtg
     3121 accttatttt aacaagtcca ggtaatcctt cagggcctcc ttgtaactcc ggaggggtgg
     3181 gaatccctgc aaccgccagt ggtagttgtc caggaccgag tacttgggcc ggggtgctgg
     3241 gctgccgaac tcctcggtgg tgaccggctg caactccacc tccactccct tcatctgatg
     3301 gatgagctgg gcatactcgt accaggaaca gtggtcactg ttggtgacgt ggtagacacc
     3361 gtaggccggt gtctttatga gatgg
//
LOCUS       DAOQVW010000129         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_32105_length_3384_cov_3.375488, whole genome shotgun sequence.
ACCESSION   DAOQVW010000129 DAOQVW010000000
VERSION     DAOQVW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32105_length_3384_cov_3.375488"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PKK85_03790"
     CDS             <1..1021
                     /locus_tag="PKK85_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNS25170.1"
                     /translation="EIVRDAEITVGVNHRGVERIMEGLPVEKANSLTEKICGICSNSH
                     IWNSCLVAEKALDIEVPERAQYIRIIMEEMERLHSHFLYLAHGNEVLGHETFAMRLFY
                     IRETVMDLLGMIGGNRVQYGAPILGGVRPRCELDDKRIQKLKDGMDLMEERLAAFADR
                     FVSDPMVMSRITDVGVISQKDALRLACTGPTLRATGVESDLRTEMKEYDPFEFDVITQ
                     DGGDVKAQLLTRVFENLESIKIIRQAIRDLPDGPITNRSWEMKDTPLTKSYIEVPRGT
                     LYHSYALEDGRVRHCVIRTPSMANIGAMQHACIGHHITDAQLSIVQCDPCFTCTDRAI
                     QIIKI"
     gene            1032..2021
                     /locus_tag="PKK85_03795"
     CDS             1032..2021
                     /locus_tag="PKK85_03795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNS25171.1"
                     /translation="MNITYSLIGVIGTLVVAFIVSLLLPGIERKFIHARIQQRVGPPI
                     TSPGVMAPLKFFFKQTIDPNSPMPKLYNALPLISLIVVLILLVILIPEMYFLGAFASV
                     IAIIGFLKVEEIMYMFMGSLSKSVLSVRMPFPDMAKGAKHPETPRTFVEELSALRAFR
                     LIAFGSFPIYIALFVSVAVTGSIYLSDIVAYQQQNGPILFTVAGILGAVVFFIGYMIL
                     LNEYPFAILKTKADVIEGPYLEYAAKYRSYVYLTRGFLMFVLASLFATLFLGIAPNIL
                     NPSFIITLVVALLFPMLMAVMSAFSPIFTYKQFYPTVAAVSILGVLAIVAAFL"
     gene            2066..2329
                     /locus_tag="PKK85_03800"
     CDS             2066..2329
                     /locus_tag="PKK85_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012956797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-converting hydrogenase B subunit P"
                     /protein_id="HNS25172.1"
                     /translation="MKIVIRPLHIISLGGYIVEWDFPYRNVIVVNPTEEFIKIEVPVF
                     NEEWLDEHRKLGLEVIPLTEEDNYLSKFRKAKARLEKLKAEMN"
     gene            2853..>3384
                     /locus_tag="PKK85_03805"
     CDS             2853..>3384
                     /locus_tag="PKK85_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HNS25173.1"
                     /translation="MKEIAINIKARNEPGVLNDISALMARCGINITYTHLFIEDKDSG
                     SIYMELEDVKNIEIFIDKIRSFEAVQEVEIHRSLSDIYGKRIIIIGGGAQVAMVAHGA
                     ITEADRHNIRGERISVDTIPLVGEKELAEAVSAVGRLPRVGVLVLAGSIMGGAITQAI
                     EEIKSEHGVIVISLNMP"
BASE COUNT          948 a          755 c          774 g          907 t
ORIGIN      
        1 cgaaatagtc cgggatgcag agataacagt tggagtaaat catcggggag tggaacgaat
       61 aatggaaggc ctccctgtgg agaaggccaa cagccttaca gagaaaatct gtgggatatg
      121 ttcaaacagt catatctgga actcctgtct ggtggcagaa aaggcactgg atattgaagt
      181 gccggaaaga gcacaataca tcaggataat aatggaagaa atggaaagac tacacagcca
      241 tttcctctat ctggcccatg gaaacgaggt tctaggtcat gaaacctttg ccatgcgatt
      301 gttctacatt cgggaaacag tgatggatct gttagggatg atcgggggga accgggtgca
      361 atatggggca cccatccttg gaggggtccg tccgcgatgt gaactggatg acaagcggat
      421 ccagaaacta aaggatggta tggatttaat ggaagaaaga ttagccgcct ttgctgaccg
      481 attcgtttct gatcccatgg ttatgagcag gatcactgat gtaggggtta ttagtcaaaa
      541 agatgcattg aggctggctt gtactgggcc tacattaagg gctacaggag ttgaaagtga
      601 tcttagaact gagatgaaag agtacgatcc attcgaattt gatgtcatca cccaggatgg
      661 tggtgatgtg aaagcccagc ttttaaccag ggttttcgaa aacctggagt ccatcaagat
      721 catccgccag gccatcaggg atcttccgga tggtcctata acaaaccgta gttgggagat
      781 gaaagacaca ccccttacca agagttatat tgaggtacct agggggacac tttatcattc
      841 ttatgccctg gaggacggcc gggtgagaca ctgtgtaatc aggacgccgt ccatggcaaa
      901 cataggtgcc atgcagcatg cctgtattgg acatcatatt actgacgctc aactgtccat
      961 tgtacagtgc gacccctgtt tcacctgtac tgatagagca attcagataa tcaagatatg
     1021 aggagattaa catgaacatc acatattccc tgataggagt aattggcacc ttggtagtgg
     1081 cattcatcgt cagcctcctg ctaccgggta tagaacgcaa gttcatccac gcccggatcc
     1141 agcaaagggt aggacccccc attaccagcc caggggtcat ggctccgctc aaattctttt
     1201 ttaagcaaac catagatccc aactccccta tgccgaagct ttacaatgca ttacccctca
     1261 tcagcttaat tgtagtcctg atactcttag taatcttgat ccctgaaatg tactttttag
     1321 gagcatttgc cagtgttatt gccattattg gatttttaaa ggtggaagag ataatgtaca
     1381 tgttcatggg cagcctctcc aaatccgttc tttccgtgcg tatgcccttc ccagacatgg
     1441 ctaaaggggc taaacatcct gaaactcccc gtacatttgt ggaagagctc agtgccctaa
     1501 gagcattccg actcatagcc ttcggttcct tcccgatcta cattgcccta ttcgtttctg
     1561 tggctgtaac gggaagtata tatctttccg acatcgtggc ttaccagcag caaaacggac
     1621 ccatcctctt caccgtggca gggatcttgg gtgcagttgt attcttcatt ggctacatga
     1681 tacttttaaa tgagtatccc ttcgccatct taaaaaccaa agcagacgtc attgagggac
     1741 cctatcttga atatgcagct aaatatcgtt cttatgttta cttgacccga gggttcctga
     1801 tgttcgtcct ggccagcctc tttgccaccc tttttttggg gattgctccc aacatcctta
     1861 accccagctt tataattacc ctggtagttg ccctgctctt cccaatgctt atggccgtca
     1921 tgagtgcctt ttcccccata tttacttaca aacagttcta tcctaccgtg gctgcggtat
     1981 caatactggg agtgttggct atagtggcgg ccttcctgta ataatgtaaa agtagttgtt
     2041 caaattccaa aaacatggtg attaaatgaa aattgtaata agacccctgc acataatcag
     2101 tttggggggt tatattgtag aatgggactt tccataccgt aacgtaattg tggtgaatcc
     2161 tacggaagag ttcatcaaga ttgaggtacc ggtatttaac gaagaatggc ttgatgaaca
     2221 ccgtaaactg ggactggaag tcattccact aactgaagaa gacaattacc tcagtaaatt
     2281 ccgaaaggcc aaagctaggc tggagaaatt aaaggcagag atgaattaac atcaagtatc
     2341 attgcatcaa tttttttttg ctccggctat ataatccact atttgttttt gtgcacgatt
     2401 ttcctgctat atcttttgca tccttcaaga ataaggtggc agggggaatt atggtaggta
     2461 tcacctggac tccggtgcca aagataaatc tacaacttac aacccataca gcaatcggat
     2521 aggtttcata tcatccaaat aatctttttt aaggggagca atgcctccca aataccccat
     2581 ggggcatgtc ttggatgaag ggctgaaaaa cccttacatc ctctataacc tgggatcttg
     2641 cccaacaact tcttccagac agattttatc agtacaactt agtccttttt ttcaatttac
     2701 cattggtgta gacgctacac gggtatcttc tagtcctctg ccgggataaa accttacaaa
     2761 aataaatctc cctatttatc aaatttatat cctgggaggc gtcatataaa ttaccaacca
     2821 gtttttttag agctcaacta acagtgatat aaatgaagga aatcgccata aatatcaaag
     2881 cccgaaatga acccggggtt cttaatgata taagtgccct gatggcacgg tgcgggataa
     2941 atatcaccta cacccacctt ttcattgaag ataaggactc aggctctatc tacatggaac
     3001 tggaggatgt taaaaacatc gagatattca tagacaagat ccgaagcttc gaagccgtgc
     3061 aggaggttga aatccaccgt tccctttcag atatttatgg taaacgtatc atcattattg
     3121 ggggcggcgc tcaagtggca atggtagccc atggtgccat caccgaagct gaccgacaca
     3181 acatccgggg ggaaaggatc agtgtggaca ccataccttt ggtgggagaa aaagaacttg
     3241 ccgaagcggt atcagcggtg ggaagacttc ccagggtggg tgttctggtc ctagccggct
     3301 ccatcatggg gggcgctatt acccaggcca tagaagaaat aaaaagtgaa catggcgtca
     3361 ttgtcatcag cctcaacatg ccag
//
LOCUS       DAOQVW010000130         3373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_32254_length_3373_cov_4.900542, whole genome shotgun sequence.
ACCESSION   DAOQVW010000130 DAOQVW010000000
VERSION     DAOQVW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32254_length_3373_cov_4.900542"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..227
                     /locus_tag="PKK85_03810"
     CDS             87..227
                     /locus_tag="PKK85_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25174.1"
                     /translation="MEAETVLRLERLVNRGMYKNVSEANRAGLALLFEAIDKGQVPTP
                     IK"
     gene            674..2719
                     /locus_tag="PKK85_03815"
     CDS             674..2719
                     /locus_tag="PKK85_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25175.1"
                     /translation="MTTPTPGPVYLPLEYEEGTPGHPYLKIEVIESFNEDKKNEGVNK
                     YHYEIFNKDGNTADILKEWSSPLSIQDPDLRSKLVFETQLRNAHIKTSFDSLKDIKPV
                     EFEYDPKEPAKEILLEYGDPIDNRREYVFDPEELAVYIDTYKYVKNKKEEEELRRVGH
                     DKIFSAYPVQITICQSPLSRETSYKLEWKAKRALYPFETEGSPMGILSILKKYGYILK
                     SYAIEDRFAKLLDIAYNEGYARTDYTMDKPGFFLFDGAIIPSMYDMTPPTPDEIRESL
                     DILERFGDSFSNDKMERDQRDKIASTIKLGLTLPFNFVRKKLGYSEVIKPQMAYGKPG
                     SGKTTINLLPAYIYGQSIDDYEKAGANILTAPRFAELMGLTTFPTMIDEAGSIFDNKD
                     LMEVNKNGLRREYVRDKLTKEGKRIREPAYSTFIYISNYPPIKDSNDGTVRRYHIHHY
                     EEIEYKDEKNDMVKSFNQRFKPRDPESPLLRLEALGRFAAAYIIHNKKILGRDMMEAG
                     QEIINEMYGFINRAPPEWITWEAETESLEDFGAHENEIILSFIRGETMTAYRDKILVD
                     DGGYGKREKLTNKDDEEAKEKIKDRYYQVAKEGIIPWLFYKESQGSFFLRSTFKDDLS
                     RSRGLSLSLKSIAQKFEGWKYSTSRDPKTKKTSKMIKIPKEYLYDHIIERVDVQDTL"
BASE COUNT         1254 a          605 c          638 g          876 t
ORIGIN      
        1 ttttttttat actcaaatcc tttttgtttg aattaatttt ttaataggag gtgaaaagag
       61 atatgcagat ctgcaaaacc gtttcaatgg aagccgaaac cgtgctcagg ttggagcgcc
      121 tggttaaccg tggaatgtat aaaaatgttt cagaagcgaa ccgcgccggc cttgctttgt
      181 tattcgaagc catagacaaa ggccaggtgc caacaccaat aaaatagaat ttaataataa
      241 accgtgtaag tagtattata aaagtatttt aaagactagt taccaaatcc tagaatagaa
      301 acaaacctca aaacatggga aaaatgggcc cggaagcggt gaagcttcca ggtggagcat
      361 aacccgcagc aacgggttat attaaaagta gttagtaata ggtagattga cttattattt
      421 aaacttttaa ttctacttga agccctccac catcccaata gctgaataat tcctattttt
      481 ccagcaaata aatattttat actaaggagg cgctaatatt ggcgaaaaca catataaacc
      541 tattaataca ccgggacacc ctccataaga tacaggaatt atataaaact gaatacccgg
      601 accatgaaag aagttttagt tcttttattg atgaaatact aattttatac ataaccgaac
      661 gggaggccac aacatgacca cccccacccc ggggccggta tacctacccc tggaatacga
      721 ggagggcact cctggacatc cttatcttaa aattgaggtc atagaatcat ttaatgaaga
      781 taaaaagaat gagggcgtaa ataaatatca ttatgaaatc tttaacaaag acgggaacac
      841 cgccgatata ctgaaagaat ggagctctcc attaagtatc caggatccgg atctaaggag
      901 taaattagta tttgaaactc aacttaggaa tgcgcacata aaaacatcat ttgatagttt
      961 aaaagacatt aaacccgtag aattcgaata tgaccccaaa gaaccagcga aagaaatttt
     1021 attagaatat ggggatccaa tcgataacag gcgggagtat gtttttgacc ctgaagaact
     1081 ggccgtttat attgatactt acaaatatgt aaagaataag aaagaagaag aagaattaag
     1141 gcgggttgga catgataaaa tattttcagc ctaccccgtg caaataacaa tatgccaaag
     1201 ccccttaagc cgtgaaacaa gttataaatt agaatggaaa gccaaaaggg ccctttatcc
     1261 atttgaaact gagggtagcc caatgggtat tttatcaatt cttaaaaaat atggctatat
     1321 acttaaaagt tatgccatag aggatcggtt tgctaagtta ttagacattg catacaatga
     1381 aggatacgcc aggaccgatt atacaatgga taagcccggt tttttcttgt ttgacggtgc
     1441 gatcattccc tccatgtatg acatgacacc ccccaccccc gacgaaataa gggaatcatt
     1501 ggacatcctg gaaagattcg gggattcttt cagtaatgac aaaatggaac gggaccagcg
     1561 ggacaagata gcaagtacca ttaaattagg tttaacattg ccctttaact tcgttaggaa
     1621 aaaattagga tacagcgaag taataaagcc tcaaatggct tatggtaagc ctgggagcgg
     1681 caaaacaacc attaacctat taccagctta tatttatggt caaagtatag acgactatga
     1741 aaaggccggc gcgaatatcc taacagcccc ccgatttgct gaattaatgg gattaaccac
     1801 ctttcccact atgatagacg aagccgggag catatttgat aataaagact taatggaagt
     1861 taataagaac ggcttaagaa gggaatatgt tcgagacaaa ttaacaaaag aaggaaaacg
     1921 gatccgggag ccagcatata gcacatttat ttatatctcc aactatccac cgatcaagga
     1981 ttctaacgat ggaacggtcc gccggtatca tatacatcat tatgaagaaa tagaatacaa
     2041 agacgaaaaa aacgacatgg ttaaatcatt caaccaacga tttaaacccc gagacccgga
     2101 aagccccctt ctcaggttgg aggcattggg ccggtttgca gctgcatata taatccacaa
     2161 taagaagatc ctagggcggg acatgatgga agccggtcag gagatcatta atgaaatgta
     2221 cggatttata aaccgtgccc ctccggagtg gataacctgg gaggctgaaa ccgaaagcct
     2281 ggaagatttt ggagcacatg aaaacgaaat catattatca tttatcagag gcgaaaccat
     2341 gaccgcctac cgggataaaa tcctagtgga tgatggaggt tatgggaaaa gggaaaaatt
     2401 aaccaataaa gatgatgaag aagctaagga aaagatcaag gatagatatt accaggtagc
     2461 aaaagaaggg atcatcccgt ggctattcta caaagagagc caaggatcat ttttcctaag
     2521 atctactttc aaagatgatc tatcccgaag ccgtggattg agtttaagtt taaaatccat
     2581 tgcccaaaaa ttcgagggct ggaagtattc cacaagtaga gatccgaaaa ctaaaaagac
     2641 atccaaaatg attaaaatac ccaaagaata tttatatgac catataatcg agagagtgga
     2701 tgttcaagac acattataga tgattaactg gtttttaatt ggtttttata attttgttac
     2761 ttttgttacc tgaatataat ttgaaactct aaaaaaaagt aacaaaaaat aacacttctt
     2821 aggtgtttaa tttataatca agacattttc cttaggattt gaaaaatgtt acttttgtta
     2881 cctgtgttac ccccgtttta ctaagatagt aaattaagtt caggtaataa ggtataatca
     2941 taaaattgtt tgtactatat aggtaactta agtaacaaaa gtaacaaata tatatataag
     3001 cctaaatcaa gttaaaaagt tacaaaccca ctaaaaacac cctattaatg ttataaacta
     3061 atataaactt gttttattat aattgaactt ggtaacatgg caaaaactgg atttaaagga
     3121 atttttaaaa acccattttg ttacctgacc cccgagtaac aaaagtaaca aagtaacaaa
     3181 aaatattaaa ccacctaaaa ccagcttaaa cccaaaataa acctttttta gatgtttctt
     3241 tattcacatg aggagtgata caactatgaa acaacataag aagatgatca cacatggaac
     3301 aagaattaca cgaaacatta agcagaaact gggatcaaac catcgaactg accaatgaca
     3361 tatatggaga tcc
//
LOCUS       DAOQVW010000131         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_32404_length_3361_cov_2.832426, whole genome shotgun sequence.
ACCESSION   DAOQVW010000131 DAOQVW010000000
VERSION     DAOQVW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32404_length_3361_cov_2.832426"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..808
                     /locus_tag="PKK85_03820"
     CDS             185..808
                     /locus_tag="PKK85_03820"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNS25176.1"
                     /translation="MKRVEIDPTQPDFPEIQKAQMVLKEGGLVVYPTDTLYGLGANIF
                     QEEAVKRVYHVKKRSPEKPLSICISRVQDLQRVAHMDLKSFQLANKLLPGPYTLVVKK
                     NPQIPYLITAGSDKVGIRIPDHPICRVLSMEFPITTTSANISGEEAPLEVDELVNLFQ
                     DQVDLYLDAGDAQPFHSTVIDLTTSPPTILREGAGMDLLNQLLDDLF"
     gene            917..1615
                     /gene="radB"
                     /locus_tag="PKK85_03825"
     CDS             917..1615
                     /gene="radB"
                     /locus_tag="PKK85_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HNS25177.1"
                     /translation="MLKELSQILSNDKIPTASPLDRLLGGGVEKGCITQFYGPPGSGK
                     TNIVLNLAVECARNNRRTIFVDTEGGLSIERVKQISGRDFEKVASQILVFEPSTFSEQ
                     DQVLKRIEEMFQSGEEADLLVVDSAVALYRVKDGDSFSLNQELGKQMGLLLRIARRFN
                     VAVVITNHVYSRFDGDGLVEPVGGTILKYRSKIMVELARGDLNGERFAVLKRHRSRPE
                     GLQTRFRIVDSGLQ"
     gene            1737..2897
                     /locus_tag="PKK85_03830"
     CDS             1737..2897
                     /locus_tag="PKK85_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNS25178.1"
                     /translation="MISPKKYAKSAKALPKGFYSANEFFNYVYNDQDMIWMGQNTNHL
                     HDEDGTMEAMLGSIKKKDYCKYPPPEGFPELKNLVQEDLGLTHGFDILITAGGTESLY
                     LCTNNILEPENNTITCDPGYLIIDNFASRFGDHVKSIPIYNQECHYKLTPQLVRENMD
                     KDTKLVSLVDPLNPLGSSYTKEELKDFADLAEDKDIYLLHDITYRDFARDHYLMAKHA
                     PEHTITVYSFSKIYGMAGLRVGAVLGTPEMMDSLRTIVINDLGTNIVAQKGAIAAVNS
                     KYEWLNRVKNTTRSNQDIIKEAVDLVEGCFIPVYPSQGNMLVIDMYESGVKPHEMVDY
                     LLEKKIFTREGSYTSKLFGHRYLRVSFSIPKHEVEYFADCFLDGMDELAGKN"
     gene            3000..3347
                     /locus_tag="PKK85_03835"
     CDS             3000..3347
                     /locus_tag="PKK85_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25179.1"
                     /translation="MVAEEGSGERLPAGELDVWLMKPDDEKGPWSVITIQIELESPLT
                     PEMILKYLGTYLEVDGMEFEVMKAPNEHLVIAPEKALQELMKSCFKLNIKLDEFNVIL
                     MPLEKFKDKIEDI"
BASE COUNT          968 a          808 c          826 g          759 t
ORIGIN      
        1 aaatccccag ctctttctat aatggaccca tccacccagg agatcaggac caggataacc
       61 agtattactc ccccggttat aagacaaacc gccaggaggg ggcccagcca gaccaggaca
      121 tcatcaaaca tataagttaa gataggtact aattctattt atcaatattt tttaatgagg
      181 acacatgaaa cgcgtagaaa tagatcccac ccaaccagat ttcccggaga tacagaaggc
      241 gcagatggtc ttaaaagaag gggggctggt ggtctacccc accgacaccc tgtacggatt
      301 aggggccaac atcttccagg aagaagcagt taaaagggtg taccacgtca agaaaaggtc
      361 cccggagaaa cccctgtcca tctgcatatc ccgggtgcag gacctgcaga gagtggccca
      421 catggatctt aaatccttcc agctggctaa caagctcctg cccgggcctt acactctggt
      481 agtaaagaaa aatccccaga taccatattt aataactgct ggctcggata aggtgggaat
      541 ccgcatcccg gatcacccga tctgccgggt gctgtccatg gagtttccca tcaccaccac
      601 cagcgccaac atctcgggtg aagaggcacc gcttgaggtg gatgaactcg taaatctctt
      661 ccaggaccag gtggacctct atctggacgc cggcgacgct caaccgttcc actccacggt
      721 gattgacctg accacttctc cccccactat cctgcgggag ggtgcaggga tggatcttct
      781 aaaccagtta ctagatgatt tattctgact attccctcat aatcagatgg actggttgaa
      841 atagatgccg tggccatagt ttcgtaggca gggttaaata ataaataacc ctgtactata
      901 taatacttaa gataccatgt tgaaagaact aagtcagatc ttaagcaacg acaagatccc
      961 cactgcatcc cccctggaca gactcttagg gggaggggtg gaaaaaggat gtatcaccca
     1021 gttctacggc ccccccggct caggtaaaac gaatatagtc ctgaacctgg cggtagagtg
     1081 tgcccggaat aatagaagaa ccatcttcgt ggataccgag ggtggcttgt ccattgaacg
     1141 ggtgaagcag atctccggcc gggacttcga aaaggtggcc agccagatcc tggtctttga
     1201 accctccacc ttctcggagc aggatcaggt tctgaaaaga atcgaggaga tgttccagtc
     1261 tggtgaagag gctgatctct tggtggtgga ctccgcggtg gctctgtatc gggttaagga
     1321 tggggattct ttcagtttaa accaggagtt gggtaagcag atgggtttgc ttttaaggat
     1381 cgcacggcgc ttcaatgtgg cggtggtcat caccaaccat gtttactccc gttttgatgg
     1441 ggatggattg gtggaaccag tgggaggcac catactcaag taccgcagca agatcatggt
     1501 ggaactggcc cggggagatc ttaacgggga gaggtttgca gttttgaaaa ggcaccgcag
     1561 ccggccagag ggcctgcaga cccgtttcag gatcgtggac tctgggttgc aatagataat
     1621 tttaaatgat tgttcactaa aaataagata aaattctaac aacctcaacc agaggaggtt
     1681 tcacaaggga taatactact tttttccctg gatcaaaatc taatgtatgt gagattatga
     1741 tttctccaaa aaaatatgct aaatctgcca aggcactgcc aaagggattt tattctgcca
     1801 acgagttttt caattacgtt tacaatgacc aggacatgat ctggatgggt cagaacacca
     1861 accacctcca tgatgaagat ggcactatgg aggccatgtt gggaagtata aagaagaagg
     1921 attactgtaa atatcctcca ccagaaggat ttccagagtt gaagaacctg gtacaggagg
     1981 atctgggact cacccacggc ttcgatatac tcatcaccgc aggggggacg gaatcactgt
     2041 acctgtgcac caacaacatc ctggagcctg agaacaacac catcacctgt gatccaggat
     2101 acctcatcat cgataacttc gccagtcgct tcggagacca tgtgaagtcc atccccatct
     2161 acaaccagga gtgccactac aagctcacac cccaattggt acgggagaac atggataaag
     2221 acaccaagtt agtatccctg gtggaccccc tgaatccatt gggatcatcc tacaccaagg
     2281 aggagttaaa agacttcgcg gatctggccg aggataagga catctacctg ttacatgaca
     2341 tcacctaccg cgattttgcc agggatcact acctcatggc caagcacgcc ccggagcaca
     2401 ccatcacggt gtacagtttt tccaagatct acggcatggc tggcctgcgg gtaggcgcgg
     2461 tcctaggtac cccggagatg atggactccc tccgtaccat cgtcatcaac gatctgggca
     2521 ccaacatagt ggcccagaag ggtgcaatag ctgcagttaa ctccaaatat gaatggctga
     2581 accgggttaa gaacaccacc cgctcaaacc aggacatcat caaggaagca gtggacctgg
     2641 tggagggatg cttcatccct gtctacccct cccaggggaa catgctggta attgacatgt
     2701 acgaatcagg ggtgaaaccc catgaaatgg tggattacct tctggaaaag aaaatattca
     2761 ccagggaagg ctcctacacc agcaaactct tcggtcaccg ttacctacga gtgagcttct
     2821 ccatacccaa acatgaggtg gagtactttg ccgactgttt cctggatgga atggatgaac
     2881 tggccggaaa aaattaactt ttatagggca acgccccatt aagtgccatg tttcatggac
     2941 ctgaggatga ctctccattt atctaagatt tcaccaacga aaaaaaaggt aatataataa
     3001 tggtagccga ggaaggatcc ggagaaagat taccagccgg ggagctggat gtatggctga
     3061 tgaaaccaga cgatgaaaaa ggcccctggt cggttataac cattcaaata gagctagaaa
     3121 gccccttaac gcctgaaatg attcttaaat atttagggac ctatctggaa gtggatggca
     3181 tggaatttga ggtgatgaag gcccctaacg aacacctggt catcgcccct gagaaagcat
     3241 tgcaggagtt gatgaaaagt tgtttcaaac ttaacataaa gttagatgaa tttaacgtta
     3301 tactcatgcc cttagagaaa tttaaggaca aaatagaaga tatttagagg gttaacaagg
     3361 g
//
LOCUS       DAOQVW010000132         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_32698_length_3338_cov_3.020713, whole genome shotgun sequence.
ACCESSION   DAOQVW010000132 DAOQVW010000000
VERSION     DAOQVW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32698_length_3338_cov_3.020713"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1250
                     /locus_tag="PKK85_03840"
     CDS             183..1250
                     /locus_tag="PKK85_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HNS25180.1"
                     /translation="MDKAIKTYGSLEKLPEIKEKGALFLCTIASTLTSHIPGITGAGA
                     TPELTDYTPAADMELIVYGEPRCLPEIPQTIVDGSAAPTPAVITKAALELADIPFLVV
                     DAGAAVKPNLPYIKVNEEPGSDIKTGRAVSNPQLIYEQGKVVGEILSRLTGHMFIGES
                     TPAGTTTALGVLQALGYDAWGKVSGSTPENPHHLKEQLVKQGLMAAGIYDQELQDPFQ
                     AVMAVGDPMIPAVAGIAAGSKVPVTLAGGTQMTAVCALLKAVNPLYSFDDLSLATTIF
                     IARDETSDINYLTRQIDDIPIIAVDPFFERSENESLKNYLNGSVKEGAGAGGAMLAAL
                     LMDNTMDDIRAMTENLCKEIF"
     gene            complement(1301..2125)
                     /gene="uppP"
                     /locus_tag="PKK85_03845"
     CDS             complement(1301..2125)
                     /gene="uppP"
                     /locus_tag="PKK85_03845"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNS25181.1"
                     /translation="MTIIQAIILGMVQGLTEFLPISSSAHLVLIPELLGVKSNLAFDT
                     LLHLGTLIAVLYYFWNDVVNMIQAFISSLFDLPSGKFREKLREDPYKRLSWLIIVGTI
                     PAGLMGVLFKDFFEGLFSSVTSVAVFMLITGFILWGVERMPRGNRDVKDISFTNSLLV
                     GIAQGCAIAPGISRSGATIATSLFLGFDREMAARFSFLLSIPAILGAALIQVKDLTTG
                     FDLSTGVFLAGLLSSVIFGYLAIRFLMGYIKKHKLTIFAYYCWIVGIIVLSSALIK"
     gene            complement(2221..3144)
                     /gene="ilvE"
                     /locus_tag="PKK85_03850"
     CDS             complement(2221..3144)
                     /gene="ilvE"
                     /locus_tag="PKK85_03850"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNS25182.1"
                     /translation="MAFDESGKIWFNGKFVDWKEANIHVLSHVVHYGSSVFEGIRCYN
                     TKQGPAIFRLKEHVDRLYNSARIYRMDIPYSQEEFLKAIVDTVQINRLKACYIRPVVF
                     RGYGELGVYPLNCPVISVIAAWAWGKYLGEEALELGVDVGVSSWRRMAPDTMPNLAKA
                     GSNYMNSQLAKMESLFNGYHEAIMLDYSGMVSEGSGENIFLVKEGILYTPPVSSSLLN
                     GITRDSIITLAQDMDLDVVEQQIPREMLYLADELFLTGTAAEVTPIRSVDQIIIGEGL
                     RGEVTQKLQDSFFKILEGKVEDRYGWLTYLK"
BASE COUNT          954 a          821 c          725 g          838 t
ORIGIN      
        1 cacacggtat cgggtgctaa agtgtcactc actagaaatg ggggctatgt ggcccccaac
       61 tccagatgca aggggagcac cttgaccaga aaaacagcac catcaaacca ttaattacat
      121 agttcatctc cataaaaacc tctaatacct atctgataca taagacccta gcagatgata
      181 acatggataa agccattaaa acatatggat ctttggaaaa actcccagaa ataaaagaaa
      241 aaggggcact ttttttgtgt accatagcca gcaccctcac ttctcacata cccggaatta
      301 cgggggcagg agcaacacca gaactaactg attacactcc cgcagctgat atggagttaa
      361 ttgtctatgg ggagcccagg tgccttcctg agataccgca aaccattgtc gacggatctg
      421 ctgcacctac cccggcagta ataactaaag ccgccctgga actggcagac atcccatttc
      481 tagtggtgga tgctggggct gctgtcaaac ctaacttgcc ctatattaag gtaaatgagg
      541 aacctggctc tgacattaaa accggacgag cggtctcaaa tcctcaactg atatatgagc
      601 aggggaaagt agtgggggag atcctatccc gccttactgg acacatgttc attggggaaa
      661 gcactcctgc cgggaccacc actgctttgg gagttctaca ggcactggga tatgatgctt
      721 ggggaaaggt cagtggtagc acaccagaaa acccccacca tcttaaagag caactggtaa
      781 aacagggcct gatggcagca ggtatctatg atcaagaact gcaggaccca ttccaggcag
      841 taatggctgt aggcgatcct atgatccccg cagttgctgg aatagcagct ggaagcaaag
      901 ttccagtaac tctggccggc ggaacccaaa tgacagctgt ctgcgccctt ctaaaagcag
      961 ttaatcccct gtatagtttt gatgatctgt ccctggccac caccatcttc atagcccggg
     1021 acgagacttc tgacatcaac taccttaccc gtcagatcga tgacataccc atcatcgcag
     1081 tggatccttt cttcgaaaga tccgaaaatg aaagcctgaa gaattatctt aatggctcgg
     1141 tgaaggaggg ggcaggagca ggtggagcta tgttggctgc tcttttaatg gataatacca
     1201 tggatgatat tagagccatg acggaaaatc tctgtaaaga gatattctaa gtacatcacc
     1261 aggaaatact ccacttcaca tatatgggtg atacatccca ttactttata agggcacttg
     1321 atagtacaat tattcccact atccaacagt aatatgcgaa gatagttagt ttatgttttt
     1381 tgatgtagcc catcaagaat ctgatggcta gataaccgaa tataaccgag gaaaggagtc
     1441 cggctaaaaa cacgccggtg cttaaatcga agccagtggt caaatctttg acttgaatta
     1501 gagccgctcc tagaatcgct ggaattgata ataaaaagct gaaacgggct gccatttcac
     1561 gatcaaaccc taaaaatagg ctagtagcga tggtagcccc cgaacgtgag atgcctggcg
     1621 cgatggcaca cccctgcgca atcccaacca gtaacgagtt tgtgaagctt atatctttaa
     1681 catcccggtt tcctcttggc atccgctcca ccccccacaa aataaaacca gttattaaca
     1741 tgaacacagc caccgacgtc actgaactga aaagaccttc aaaaaaatct ttgaacagca
     1801 cccccatcaa gccagctggg atggtgccta ctatgataag ccaggacagg cgtttgtagg
     1861 gatcttccct cagcttctcc ctgaacttcc ctgatggtag atcaaagagg ctggagataa
     1921 aggcctgtat catgttcacc acgtcattcc agaagtagta caacacggca atgagagttc
     1981 ctaaatgcag cagagtgtcg aatgccaggt tagatttcac tcccaaaagt tctggaatca
     2041 gaaccaggtg tgctgaacta cttattggta gaaattctgt taaaccctga accattccca
     2101 ggattattgc ttgaatgatt gtgatgtcca ttattttcac cttgatagga ttagaatcaa
     2161 ctcattagat acaaaaaatg ggaacttata agttttaact tataagttat tatccggttt
     2221 ttatttaaga taggtcagcc agccgtacct atcttccacc ttaccttcca agattttgaa
     2281 aaagctatcc tgtaacttct gggtgacttc gcccctcagg ccttcaccaa tgatgatctg
     2341 atccacggaa cgtatgggtg taacttcagc tgcagtgccg gtcaggaaca gttcatctgc
     2401 cagatacaac atctcccggg ggatctgttg ttcaaccaca tccagatcca tatcctgggc
     2461 caaggtgatt atggaatccc gggtaattcc atttaaaagt gatgacgaca cggggggtgt
     2521 gtagagtatg ccctccttta ccaggaagat gttttctcca ctgccctcac tgaccatgcc
     2581 actgtaatcc aacattatag cctcgtggta gccgttgaaa agggactcca tcttggctaa
     2641 ctggctgttc atgtagtttg aaccagcttt ggccagattg ggcatggtat ccggggccat
     2701 cctcctccag gaagaaaccc ccacatccac ccccaactcc agggcttcct ctcccaggta
     2761 cttaccccag gcccaggcag cgataactga tatcacagga cagttaaggg ggtaaacacc
     2821 caactctcca tatcctcgga agaccacggg tcggatgtag catgccttca acctgttaat
     2881 ctgaaccgtg tctacaatgg cctttagaaa ttcttcctgt gagtagggga tgtccatcct
     2941 gtagatcctg gcagagttgt acagacggtc cacatgctct ttcaagcgga aaatggctgg
     3001 gccttgcttg gtattgtagc agcgaattcc ttcaaataca ctggatccat agtgtacaac
     3061 atgagataat acgtgaatgt ttgcttcttt ccagtcaacg aactttccat tgaaccatat
     3121 ttttcctgat tcatcaaagg ccatgttatc cctccagttc ttcttgacta tgctgccata
     3181 aaaattcttt tataattttt acacgagccc acttatccat ggccagtctt aactagtaaa
     3241 gtataaatac gctgttcacc aaaccagagg aatgttcaaa tcagggccgg tggtctaggg
     3301 gtatgatacc tcgcttacaa cgaggtgatc aggagttc
//
LOCUS       DAOQVW010000133         3320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_32950_length_3320_cov_3.652067, whole genome shotgun sequence.
ACCESSION   DAOQVW010000133 DAOQVW010000000
VERSION     DAOQVW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32950_length_3320_cov_3.652067"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKK85_03855"
     CDS             <1..451
                     /locus_tag="PKK85_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNS25183.1"
                     /translation="LQEGPMDEFAYVELDGDIAVIGNGAGLTLSGMDMLQIYGGKPAT
                     FLDIGGGASQQNIARALNLVLSNPQVKVVFLNVLGGITRADDVAMGIIDVMSRAERMV
                     PLVIRLTGTNEEEGQRILKKAGVSFETSMEAAAQKAVEILDGIESKN"
     gene            complement(477..1559)
                     /locus_tag="PKK85_03860"
     CDS             complement(477..1559)
                     /locus_tag="PKK85_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25184.1"
                     /translation="MLIAQNHLQFIIEVGLMIHVGIILLFNMVAIPLSMVLFISMVLT
                     ILLAGLFSLDAAFLFLPYLTHHEFTHPFGAVAVFAWVTLTASASLLSEVDIKSSSIIA
                     LSWILFVVIGVAGMLMHRSFLILWVLGWIMGTIIMSKSFKRSVKITPKRIGMVIIAAL
                     GAFGVLEVLSRIFNAPVLSPLVRLSRLEQFAIPSLKMVIKNTMFWGHVQGSCFWGADC
                     LGGSDGYLSLPMSFINLLTLPYPLFFGVLVTKKDYVDYMLPGIFGVAFDFGYMGLLLL
                     MGWVLLVTCNGFYALREYRKKRKNGSRMYLGREALLIGALSAFLVQTIVGLFLFNRSF
                     NGSAMVTYLVLSALVMAHVVQIRRRM"
     gene            complement(1706..2554)
                     /locus_tag="PKK85_03865"
     CDS             complement(1706..2554)
                     /locus_tag="PKK85_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HNS25185.1"
                     /translation="MILIILVVALSPVYGAQGLAITYGETTYSNSAYKDSMYNYFQTH
                     TDKDLKDVTYRVITASEVNAISKDITGRNYKSSQIYSCALVDLSYSKGIKIVVDKSKV
                     TTVTSKMYATALKSSGIENGYVVVSSPTVATGESALAGVLKSYEIAVGTSIPEEAKKA
                     ATEELYVETQVVNQTGQNPDAIAELFEKTKEEVQKQKLEDPAQIKIIVINMANTLNIN
                     LSDQQAQEIADAISNSQKAQTNLTEFKNQLQQATQQASDAQGVLAQISAFLENLFNYL
                     QGLLGQ"
     gene            complement(2659..>3320)
                     /locus_tag="PKK85_03870"
     CDS             complement(2659..>3320)
                     /locus_tag="PKK85_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS25186.1"
                     /translation="GPYDEPKIIIDQGIKAQRSLLVGYFGNDKANQRKVQQAQHPNNA
                     AISLAGEPLLYPALDDLLIEFRRRKFTTFLVSNGLTPEKLENLLVEPTQLYISLDAPN
                     QRVYQDLCQPQVKNGWQKLKQSLDMLSSFDCRTVIRMTCVKDYNMFHPQDYADLINRS
                     DPDLVEIKAYMYVGNSRQRLELDNMPSYLQMQNFAQSIAERCDKEIINESRVSRVLLL
                     G"
BASE COUNT          904 a          730 c          752 g          934 t
ORIGIN      
        1 cctccaagaa ggtcctatgg atgaatttgc ctacgtggag ctagatggtg acatagctgt
       61 cattggcaac ggtgctgggc tcaccctcag tggaatggac atgttacaaa tctatggtgg
      121 taaaccggcc actttcctgg atattggtgg gggtgcctcc caacaaaaca tagcccgggc
      181 tctcaatctg gtcctatcaa atccccaagt taaagtagta tttttaaacg ttttaggagg
      241 aattacccgg gcagacgacg tggccatggg gattatcgat gtaatgagca gggcggaaag
      301 aatggtacca cttgttattc gcctcaccgg taccaatgag gaagaggggc agcgaattct
      361 aaagaaagct ggggtaagtt ttgaaacttc catggaggcc gcagcccaga aggcagtgga
      421 gatactagat ggaatagaat caaaaaatta aataaaattt ttagaagatc acccggtcac
      481 attcttcgcc ttatctgaac tacatgggcc atcaccaggg cggatagtac taggtacgtc
      541 accattgctg atccattaaa gcttcgatta aacaggaaca aacccacaat ggtctgaacc
      601 aaaaaggcag ataaagcccc gattagtaat gcttcacgcc ccaggtacat cctactgcca
      661 ttttttcttt ttttacgata ctctcgcagt gcataaaatc cgttgcaggt gaccagaagc
      721 acccaaccca tcaggagcag cagacccatg tatccaaagt caaatgcgac tccgaatatg
      781 cctggaagca tgtaatcaac atagtccttc ttggtgacca gcaccccgaa aaacagggga
      841 tagggaaggg ttaagaggtt tatgaagctc atgggtaggg acaagtaacc atcagatcct
      901 cccaggcaat ccgcacccca gaaacatgat ccctgcacat gcccccagaa catggtattt
      961 tttataacca ttttaaggct gggtatggca aattgttcta gcctacttaa tctcaccaag
     1021 ggacttaata ccggggcatt aaatattcgg gagagtactt ccagcacacc aaaagcaccc
     1081 agagccgcga tgatcaccat tcctatcctc ttgggtgtta tcttcacact tcttttgaag
     1141 cttttagaca ttatgatggt gcccattatc cagcccagca cccacaaaat taggaaggaa
     1201 cggtgcatta acatcccggc tactccgatt accacgaaaa ggatccagga tagggcaatt
     1261 atggatgaag acttaatatc cacttcgctt aggagactgg ccgaagccgt cagggtaacc
     1321 caggcaaaca ccgccacggc tccaaagggg tgggtgaact cgtggtgggt taaatagggt
     1381 aaaaacagga aggcagcatc taaactgaag agcccggcta gtaaaatagt cagaaccatg
     1441 gatatgaaga gcaccatgct cagaggaatg gccaccatat tgaaaagcag tatgatgccc
     1501 acgtggatca tcaatcccac ttctattatg aactgcagat ggttttgggc tattaacatg
     1561 atatcactgt tactaacatt cccattttct gtggctcccc ttataattct attgttttta
     1621 tgatactaat ttaatctgat taccagcgtc ttttttggat tattgggagt ttgaatgaag
     1681 gatggcattc gattaaagaa agatttcatt ggccaagaag tccctgcaag tagttgaaca
     1741 agttctccag aaatgcactg atctgggcca gcacaccctg ggcgtcgctt gcttgctgag
     1801 tggcttgttg taactggttt ttgaattcag taaggtttgt ttgggctttt tgggagtttg
     1861 aaatagcatc tgctatttct tgggcttgtt ggtcgctgag attgatgttc agggtgttgg
     1921 ccatgttgat gacgatgatt ttgatctggg cggggtcctc aagtttctgc ttttgcactt
     1981 cctctttggt cttctcaaaa agttcggcta tggcatctgg attctgtcca gtctgattta
     2041 caacctgagt ttccacgtaa agttcctcag tagcggcttt cttagcttct tcagggattg
     2101 atgttcctac agctatttca taggacttta gaacaccagc taaggcagat tctccagtgg
     2161 ccacggtggg ggaggaaacc accacgtaac cattctctat ccccgatgat ttcagggcag
     2221 tggcatacat cttggatgtt acagtagtta ctttactttt atccactacg atcttgatgc
     2281 ccttactgta gctgagatcc accagtgcac atgaatagat ttggctggac ttgtagttac
     2341 gtccagtaat atctttggag atggcgttaa cttcagaagc agttatcacc ctataggtga
     2401 catcttttaa atctttatca gtgtgggtct ggaaataatt gtacatagaa tctttatagg
     2461 ctgaattgga gtaggttgtc tctccatagg taatggctaa tccttgggca ccgtagaccg
     2521 gagacagggc cactaccaag ataattaata tcaaacacca tcttctcact aaaaccacct
     2581 caccatttca ttctcatcag gacatcttta aatcatttgt tctatttcaa tttattccat
     2641 agaaactcaa ataaataatt accctagtaa cagcacccta ctgacccggg attcatttat
     2701 aatttcttta tcacaccgtt ctgctatgga ttgggcaaaa ttctgcattt ggagatagga
     2761 tggcatatta tccagttcca gtcgttgcct agaattaccc acgtacatat aagctttgat
     2821 ctccacaaga tcagggtcgc tacgatttat taggtcagca taatcctggg gatggaacat
     2881 gttgtagtcc ttaacgcagg tcattcttat cacggttcga caatcaaagc tggataacat
     2941 atccagggac tgttttagtt tttgccagcc atttttaact tggggttggc aaagatcctg
     3001 ataaaccctt tggttgggtg catccaggga tatgtaaagt tgggtaggct ccaccaacag
     3061 attttctagt ttttccggag tgagaccatt ggaaaccagg aatgtggtaa attttcgcct
     3121 acggaactct ataagaaggt cgtccaaggc agggtagagt aaaggttctc cagccaggga
     3181 tatggctgca ttgttggggt gctgggcttg ctgtacctta cgctggttgg ccttatcgtt
     3241 accaaagtat ccaaccagaa ggcttcgctg cgcttttatt ccctgatcta ttatgatttt
     3301 aggttcatca tagggcccct
//
LOCUS       DAOQVW010000134         3318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_32983_length_3318_cov_4.120441, whole genome shotgun sequence.
ACCESSION   DAOQVW010000134 DAOQVW010000000
VERSION     DAOQVW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32983_length_3318_cov_4.120441"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..69
                     /locus_tag="PKK85_03875"
     CDS             4..69
                     /locus_tag="PKK85_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNS25187.1"
                     /translation="MFLHLGANVIAYLLGMKKVWY"
     gene            complement(176..862)
                     /locus_tag="PKK85_03880"
     CDS             complement(176..862)
                     /locus_tag="PKK85_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25188.1"
                     /translation="MVMDKNLKILLLAVVFLAVIFLWANISEKSQLGSDNRGSVTKDV
                     YSHYGTPTVRIAVVTGMHPREGLATNMVPHAIKLFALLNQVEVADYHVRVDDKPDDFT
                     IGRNNGEGLVAQYAVPDIVKSNYQLVIVSHDHEPGYGEGYYIATPTHDVKSVSIGNAV
                     HQILTYFNFYPGSSDVKSQASSITLVDYPITLAGIPVLVYEMPEWSNFFEAMYHTYKL
                     MQASSMALSS"
     gene            complement(874..1917)
                     /locus_tag="PKK85_03885"
     CDS             complement(874..1917)
                     /locus_tag="PKK85_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNS25189.1"
                     /translation="MKIAGLDIGGATTDLAVVEFSASADIKNVRVDNEYLPIWMEKEN
                     LGNVIKNMLGSDFHDLDAIGVSMTAELADAYATKKEGVLDIVKRIRELTSIPMGFVGQ
                     NGIMGHHEVLEKPLDVAAANWIATVPLAAMISPDCILIDTGSTTTDIIPIRDGQECAQ
                     GRSDLERLATGELVYTGLLRTNVATLVDRMPLRDIWVRVASELFAITADIHIILGNIE
                     EREYSCPTPDGSGESRTECMQRMARVVCSDLDVLAEEEVEGLASYVYQKQLERITEAL
                     LEVAESNDLEEVVVTGLGMDLVGREAANLLGLKVTGMNELLTREECISAPAVGTALLM
                     EEFLRSKSFNENI"
     gene            complement(1914..2939)
                     /locus_tag="PKK85_03890"
     CDS             complement(1914..2939)
                     /locus_tag="PKK85_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNS25190.1"
                     /translation="MKLLIIEYAMASGNEDPSIAIEGRTMLQGLLEDFNNENTSYLIS
                     YHSLPLDGGRCHAKKIFDDVEEWLKEDLSHYDACLPIAPEEDFILYRITRAIEEQGLK
                     VIGSLSKGVLTCSDKWETYQALKKVSPLISTDRIFFEDLDGNSKHVKAIPDFEKLKVA
                     KPVDGVSCSGIRLVDSQRKLEEAAIQIRKNSSFPFFLLQDYIEGDSCSVSLLTDGRNA
                     MPISLNLQNIKINDSYLNYNGGKTPLEHPLKCEAFAVAKKAVESIRGLCGYVGVDLIL
                     GDQVHLVEVNSRLTTPYIALRKLVNFNLGQAVMDAAQGTLPSTIQLEGNAYFSKNNHE
                     MNVSVIK"
     gene            complement(2936..>3318)
                     /locus_tag="PKK85_03895"
     CDS             complement(2936..>3318)
                     /locus_tag="PKK85_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNS25191.1"
                     /translation="EALMEKDKKLRGSEFGILGVAYKGNVADARESPAEDLIRELLSS
                     GGVVYAHDPYVPSELVESFGAKSVSLERVLDCDCVILMTGHDIYSHLEPSMIKKGVFI
                     CTQPILEPEKFRNADVVFRGVGRS"
BASE COUNT          945 a          782 c          605 g          986 t
ORIGIN      
        1 agtatattcc tgcatttggg ggcgaatgta atagcctacc tgttgggtat gaagaaagtt
       61 tggtactgag atgataaata tgaagaattc gattatgaac tttcctttta tccatgattt
      121 ttattcagaa aaacctattt aaaaaaagaa ttatcactat tattttttta cacagttaag
      181 aactcagcgc catagaagag gcctgcatta acttgtaagt atggtacatg gcctcaaaga
      241 agttactcca ttctggcatc tcgtagacca agactggtat tcctgcaagg gtgatggggt
      301 agtcaaccag ggtaattgaa cttgcctggg atttaacatc actggatcca ggatagaagt
      361 tgaaataggt cagtatctga tggactgcat ttccaattga aactgatttg acgtcatggg
      421 tgggtgtggc aatataataa ccttcaccat aacccggttc atggtcatgg cttactatta
      481 ccagttgata attagactta acaatatcag gtacagcata ttgggcaacc agcccctccc
      541 cgttgttacg tcctatggtg aagtcatctg gtttatcatc caccctcaca tgataatcag
      601 ctacttccac ctggtttaat agagcaaaaa gttttattgc atgcggaacc atgttggtgg
      661 ctaagccctc cctggggtgc atcccagtaa ccactgcgat cctgactgta ggagttccat
      721 aatgtgaata aacatcttta gttacggatc ccctattatc ggagcctaac tgggattttt
      781 cactgatatt ggcccataag aaaattacag ccagaaatac cactgcgagc aggagaattt
      841 tgagattttt gtccatcacc atacaattcc ttattagata ttttcattaa agcttttact
      901 tctcagaaat tcttccatta aaagggcagt tcctactgca ggagccgaaa tacactcttc
      961 tctggttaat aattcattca tacctgtcac cttaagtccc aggaggtttg cagcctctct
     1021 accgacaagg tccataccta atccagttac cactacttct tccagatcgt tactttcagc
     1081 aacttctaga agggcttcag tgatcctctc caactgtttc tggtacacgt aagatgctaa
     1141 cccttccacc tcctcctcag caagaacatc cagatcagaa catactacac gggccatcct
     1201 ttgcatacac tctgttctag attctcctga tccatcagga gtggggcatg aatattccct
     1261 ttcttcaata tttcccagga tgatatggat atcagcggtt atggcaaaca actccgatgc
     1321 caccctgacc cagatatccc ggagaggcat ccggtctacc aaggtggcta cattggttct
     1381 taaaagtcca gtgtaaacca gttctccagt tgccagtctt tctaaatctg accttccctg
     1441 ggcacactcc tgaccatccc ttatgggtat gatatcggtg gtggtgctgc cagtatcaat
     1501 caggatacaa tccggtgaga tcatggcagc caacggaacg gtggcgatcc agttagcagc
     1561 tgcaacatcc aagggttttt ccaggacttc gtggtggccc attataccat tttgccccac
     1621 aaaacccatg ggaatactgg taagctcacg gatccttttg actatatcca aaacaccttc
     1681 cttcttagta gcataggcat cagctagctc tgcggtcata gacaccccta tggcatctaa
     1741 atcatgaaaa tcagatccta acatgttttt tataacatta cccagatttt ccttctccat
     1801 ccatatgggc agatactcgt tatccactct cacatttttt atatcagcag aggctgaaaa
     1861 ctcaacaact gccagatcag tggttgctcc accaatatct aatcccgcaa ttttcattta
     1921 atcaccgata cattcatttc atgattattc ttggaaaaat aagcgttccc ctccagttga
     1981 atagtagagg gtaaagtgcc ctgtgctgca tccataacag cctgaccaag attgaagttc
     2041 actagtttac gaagagcaat gtagggagtg gtgagtctgg agttaacttc caccagatga
     2101 acctgatcac caagtatcag atccacccct acataaccac acaatcccct tattgattca
     2161 acagcttttt ttgcaacagc aaacgcttca catttaagtg gatgttctaa aggagttttt
     2221 ccaccattat aattcaaata tgaatcattt attttgatat tttgtaagtt aaggcttatg
     2281 ggcattgcat tccggccatc ggtcaacaaa ctaacactac aactatctcc ttctatgtaa
     2341 tcttggagta aaaaaaaggg aaaagagctg ttcttcctga tctgtatagc ggcctcctct
     2401 aatttccttt gagaatcaac tagccgaatt ccagaacagg aaacaccatc cactggctta
     2461 gctaccttca gtttttcaaa atcaggtatt gcctttacat gtttggagtt tccatccaga
     2521 tcttcaaaaa atattctatc agtgcttatc aggggagaaa ctttttttag ggcctgatag
     2581 gtttcccatt tatcagaaca ggttaaaact ccttttgata gagaacctat tacttttaat
     2641 ccttgttctt caatggcccg ggttatcctg tagagaatga aatcttcttc aggtgcaata
     2701 ggtagacatg catcgtaatg ggataaatct tcttttaacc attcctccac gtcatcaaag
     2761 attttcttcg catgacacct tcctccatct aaaggaagtg agtggtaaga gattaaatat
     2821 gacgtatttt cattgttgaa atcctccaat agaccctgta gcatggttcg accttcaata
     2881 gctatggaag gatcttcatt tccactggcc atggcgtact ctataattaa taatttcaag
     2941 accttcccac acccctaaag acaacatcag catttcgaaa tttttcgggt tctaaaatgg
     3001 gctgagtaca aataaaaacg ccttttttga tcatagatgg ttctagatgg ctataaatat
     3061 cgtgaccagt catgagaata acacaatcac agtccaatac cctctccaga ctcacagatt
     3121 tagctccaaa ggattccact agctcggaag gtacataggg atcatgagca tacaccactc
     3181 ctccggagga taataattct ctgatcagat cttctgctgg tgactccctg gcatcagcca
     3241 cgtttccctt gtaagcaacg cccaggatac caaattcaga gccccttaac ttcttgtctt
     3301 tctccattaa cgcttcac
//
LOCUS       DAOQVW010000135         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_33311_length_3296_cov_3.057390, whole genome shotgun sequence.
ACCESSION   DAOQVW010000135 DAOQVW010000000
VERSION     DAOQVW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33311_length_3296_cov_3.057390"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..817
                     /gene="pyrI"
                     /locus_tag="PKK85_03900"
     CDS             344..817
                     /gene="pyrI"
                     /locus_tag="PKK85_03900"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNS25192.1"
                     /translation="MKTPKELKVTPIRNGTVIDHITANKALHVLKILNLPSQDTSVTI
                     GMNVKSTQMGAKDLVKIEGRELRSGEVDKIALIAPHATINIIRDYEIVTKGKVNLLEK
                     ISGIINCGNPNCITNTGEPVDSLFYVTSQEPLVVRCYYCERAMDQSEIEEQFGPL"
     gene            900..1310
                     /locus_tag="PKK85_03905"
     CDS             900..1310
                     /locus_tag="PKK85_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNS25193.1"
                     /translation="MQMFRQMVDVHTSKRLEMVDITRLVEEAVESSNLKEGLVNIYSR
                     HSTSAVVINENESGLKEDFLVALDKLVPTGAGYLHDRIDHNADSHIRGFLVGGSQTVP
                     FQEGRLMLGTWQSIFFVELDGPRHRTLTVTVTGQ"
     gene            1621..2184
                     /locus_tag="PKK85_03910"
     CDS             1621..2184
                     /locus_tag="PKK85_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNS25194.1"
                     /translation="MSVTMIIGIIVLLIIIGILVYFVSIYNSLVKLRNRVKNAWSQID
                     VQLKRRTDLIPNLMETVKGYAAHEKGVLENVTRARANLMNAQTPQENAEANNVLSGAL
                     KSLFAVAENYPDLKASQNFQDMQRQLSETEDKIAYSRQFYNDTVLMYNNKIQMFPSNL
                     VASQFNFLEEEFFEVVDTEREVPEVKF"
     gene            2219..>3296
                     /locus_tag="PKK85_03915"
     CDS             2219..>3296
                     /locus_tag="PKK85_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNS25195.1"
                     /translation="MRRDIRIIKNIQLVVIILLLFVAGISAGLGVILPGDRSYSIPLM
                     DMDLYPQEDGSLHVKETIHYSFKGTYKGVYRDIPLKGTQKIENVNVSTQGAYSRFTET
                     NSSGKRQVKVYLYSDPKRTTPITDKDVDVTIEYDFAPMVKFYNDVAELQYKLVGEYWE
                     VDIGQVNAKIHLKSKDGVKYWLNPPYYNESSAWQGNTLQLTSTKIPSSDFYELRMLIP
                     KGQFDADPKNGIIINQNALEEIESIQNKYQDDINFKTNLYSFLTVLLLLACFIPILIY
                     YRFGREPKIDYQADYERDLPTDDPPAIVNAICGPGFSKRIGEPDMDGFKATIMDLLNR
                     KYLLFEKGGPEDTSPSDEMTFKFNP"
BASE COUNT          959 a          762 c          791 g          784 t
ORIGIN      
        1 aaagtactag gaagggtaaa aaaggttcta gaactaagaa gcgtaaaagg aagtgaacag
       61 cacgcctgtc tactttttct ctcttctttg gtagtaggac ctggccattt cgtagagggc
      121 ggcacccact acagagatga aaactgataa gataagtgcg gtgctggtgt ccaatctgat
      181 caatccggtt tctgctgttt acaactagca attttttttt atgctctgat tcgttgggga
      241 tggggataac gtattactct tccccattgt ttctcttctg gacaaagtta tattaactta
      301 acccccccat acacatcctt aggataggag gggatgagta gctatgaaga ctcccaagga
      361 actgaaggtc acacctatac gcaacggcac ggtcatcgac cacatcaccg ccaacaaggc
      421 cctgcacgtc ctgaagatcc tgaacctgcc cagccaggac acgtcagtga ccattggtat
      481 gaacgtcaag tccacccaga tgggtgccaa ggacctggtt aagatcgaag gccgggaact
      541 gagatcagga gaggtggata agatagccct catcgcaccc cacgctacca tcaacatcat
      601 cagggactat gaaattgtca ccaagggcaa agttaatctc ctggagaaga tcagtgggat
      661 cataaactgc ggcaacccta actgcatcac caacaccggg gaacccgtgg actccctgtt
      721 ctacgtcacc agccaggaac ccctggtggt gcgttgctac tactgtgaaa gggccatgga
      781 ccaatccgag atcgaggaac agttcggccc attgtagggg gatcttaggg tcctttatct
      841 tcacctgatt aaccaatccc tagttttggg gaatgagaaa atagaggggg ttttaatgga
      901 tgcagatgtt taggcagatg gtggatgtgc acaccagtaa gcgcctggaa atggtggaca
      961 tcacccgact agtggaggag gccgtggaga gtagcaactt aaaggagggc ctggtgaata
     1021 tttacagccg ccactccacc tctgctgtgg ttatcaacga aaatgaatcc ggactgaagg
     1081 aagacttcct ggtggccctg gataaattgg tacctaccgg ggcaggatac ctccacgacc
     1141 gcatagacca caacgccgac tcccacatcc ggggcttcct ggtgggagga tcccagaccg
     1201 tgcccttcca ggaggggcgg ttgatgctgg ggacctggca gagcatattc ttcgtggaac
     1261 tggacggacc acgccaccgg acccttacag tgacagtcac ggggcagtga agtttagaag
     1321 cccatccagg atatcacggg gttctatacg gttaaggata ggtgcagctc ctttttttct
     1381 tcccttttat ctggaaacct tactataggg aaaaactggt gtaagaggta cctgtaccca
     1441 tacatctcca gctggggggg agtggatggg gttggtggaa aacccgccat atggaagtag
     1501 gcccagcact ttttttttaa agctactccc tgaaaaaata gtctaagcac accaacgtat
     1561 attagcttag aggacaaaat aggattttac agccaacact tcacatgggg tgatatgtaa
     1621 atgtcagtaa cgatgatcat aggtataatt gtactattaa taataattgg aattctggtc
     1681 tactttgttt ccatctacaa cagcttggta aaacttcgca accgggttaa aaatgcctgg
     1741 tcccagatcg atgtgcagct taaaagaaga actgacctga tacctaacct gatggagacg
     1801 gttaaaggct acgctgccca tgaaaaaggt gtcctcgaaa atgtgaccag agcccgggcc
     1861 aatctgatga atgcacagac cccccaggag aacgctgagg ccaacaacgt gttgtccggg
     1921 gctttaaagt cattgtttgc ggtggcggag aactatcctg atttaaaggc cagccagaat
     1981 tttcaggaca tgcaaagaca actatccgag accgaagaca aaatagccta ctcccggcag
     2041 ttctacaacg acacggtgtt gatgtacaac aacaaaatac agatgttccc cagtaacttg
     2101 gtggccagcc agttcaactt cctggaggaa gagttctttg aagtagttga tactgaaagg
     2161 gaagttccag aagttaaatt ctaaatttca aggagcacgg atatccgggt gaactaccat
     2221 gagaagggac attcgaatta tcaagaacat tcaacttgtt gttatcattt tgctcttatt
     2281 tgtggctgga atatctgctg gtttaggagt gatccttcct ggggatcgga gttactccat
     2341 ccccttaatg gacatggacc tgtatccaca ggaggatgga tcactccatg tgaaggaaac
     2401 aatacattac tccttcaaag ggacttataa gggagtctac cgggacatac ccttaaaggg
     2461 aacccagaaa atagagaatg ttaacgtatc cacccagggt gcgtattcca gattcaccga
     2521 gacaaatagc agtggcaaac gccaagtaaa ggtctaccta tattcagatc ctaagaggac
     2581 cacccccatc actgataagg atgtggatgt aaccattgaa tacgactttg cccctatggt
     2641 taaattctac aacgacgttg cagagttaca gtacaaactg gtgggagagt actgggaagt
     2701 ggatataggc caggtaaatg ccaagatcca tcttaagtcc aaggatggag tgaagtactg
     2761 gctgaacccc ccctactaca atgaaagttc cgcctggcag ggtaacaccc tccaattgac
     2821 cagtacaaaa atcccctcct ctgattttta tgagctaagg atgcttatac ccaagggcca
     2881 attcgatgct gatccaaaaa atggaataat cattaatcaa aatgcattgg aggaaataga
     2941 atccatccag aacaagtacc aggatgatat aaatttcaaa accaatctgt actcgttttt
     3001 aacagtccta ttgctcttgg catgtttcat acccatcctc atctactaca gatttggtag
     3061 ggaacctaaa attgattacc aggcagatta tgaacgggat ctcccaaccg atgatccgcc
     3121 ggccattgtt aacgccatct gtggccctgg attttccaaa agaatagggg agcctgatat
     3181 ggatggattt aaagccacca taatggattt gctcaacagg aagtatctgc ttttcgagaa
     3241 aggcggccca gaggacacca gcccatctga tgaaatgacc ttcaagttca accccc
//
LOCUS       DAOQVW010000136         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_33440_length_3288_cov_3.483761, whole genome shotgun sequence.
ACCESSION   DAOQVW010000136 DAOQVW010000000
VERSION     DAOQVW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33440_length_3288_cov_3.483761"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..607
                     /locus_tag="PKK85_03920"
     CDS             401..607
                     /locus_tag="PKK85_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25196.1"
                     /translation="MVLGIEDPWIWSAYIGCILAMLLCVVYGVVNWNKGAEDEEEQIK
                     EEMEWHKKEKEMEEKELGLWDEEG"
     gene            659..2254
                     /locus_tag="PKK85_03925"
     CDS             659..2254
                     /locus_tag="PKK85_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNS25197.1"
                     /translation="MDIFILSIVVLIYLLMVGYVGYVAWKRTKSADDYMVAGRKTHPY
                     IMALSYGATFISTSAIVGFGGTAGVYGMGLLWLTFLNILVGIFIAFVFFGKRTRKMGH
                     NMGALTFPEFLSRRFNSKFIQYFSGAVIFLGMPLYASVVLIGMARFVETTLQIDYNFA
                     LVGMALIVAVYVIFGGLRGVMYTDALQGTIMFFGMLFLLASTYWVLGGVVDANQALTN
                     LINIVPASATAKATATGFTGWTSMPSLGSPFWWTLVSTLILGVGIGVLSQPQLAVRFM
                     TVKSNRELNRAVLIGGIFIFMMTGTAFVVGALSNVYFFDTVGKLAIAVAGGNADKIIP
                     TFIGGAMPLWFAYLFMITLLSAAMSTLSAQVHVQGTALGRDIYETVTSKTGGSSVMIA
                     RAGIAIAVIIAVILGFILPANIIAVGTAMWFSITAASFLSLYIFALFWKRSTKVGAIA
                     GLLTGTFFSMFWILFGYQKSAAALGICQAITGNAVILTTAPWPTVDPIVIGLPLAFVV
                     TVVVSLLTKPPAQEHMDLCFKGI"
     gene            2494..2733
                     /locus_tag="PKK85_03930"
     CDS             2494..2733
                     /locus_tag="PKK85_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNS25198.1"
                     /translation="MRIIEEIIGKEVLDSSAVVIGKVRDLELNVETNQIEAFILGKGG
                     ISEGLGLSKGETVVPYDMIKQIGDKILLKNASETL"
     gene            2762..2908
                     /locus_tag="PKK85_03935"
     CDS             2762..2908
                     /locus_tag="PKK85_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNS25199.1"
                     /translation="MEISGICPICGKQGKMYPCQLCGSRVCSECYHHQRGVCRLCRRG
                     LRMD"
     gene            2914..>3288
                     /gene="pyrE"
                     /locus_tag="PKK85_03940"
     CDS             2914..>3288
                     /gene="pyrE"
                     /locus_tag="PKK85_03940"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNS25200.1"
                     /translation="MDELKRNLLKLLTTHQVIQFGKFTLASGRESDYYVDMKKAITDP
                     GILGEIGQMMAGKITREDADKIAGPALGAVPIATAVSLATGIPMLMIRKSRKGYGTSK
                     LIEGDLEEGDRVVVVEDVTTTGN"
BASE COUNT          874 a          681 c          809 g          924 t
ORIGIN      
        1 ggttagtcat ggattcagag gatcgtttta aaatcaagat ctttccatcg tcatcggtga
       61 tcagggcccg tacagcaagg ccaaaaacta gttctatcat cttggcattc ctatcatttt
      121 tataataatt gtatacacta aactgtataa tggttgaggt tatctacccc atgcttattt
      181 aaaaaagata cccgggacct aataatcatt ttttcatgaa taaacaaggt gaatttggtt
      241 ttttatatta acacgcattt tatgtggcta attctaggct ggctccccat acttattata
      301 gatatttata atagactatt tcacccaatt aataatgata aactgagttc ttttttaaac
      361 tcaaggttta tcatatatat tatggaagag gtgcaagtaa atggttttgg gaattgagga
      421 tccatggatc tggagcgcat acattggctg cattctggcc atgcttttat gtgtagttta
      481 tggtgtcgta aactggaata aaggcgctga agacgaggaa gagcagatca aggaagagat
      541 ggaatggcat aagaaggaga aggaaatgga agaaaaagag ctcgggctct gggatgagga
      601 aggctgacta ttccaagttt tgcccattct tgttcttcat ttaaaggggg tgtttttagt
      661 ggatattttc attttaagta ttgtagtttt aatttatctt ttgatggtgg gttacgtggg
      721 ctacgtggcc tggaaacgta ccaaaagcgc cgatgattac atggtggctg gtcgtaaaac
      781 ccacccttat attatggccc tgagttatgg ggctaccttc atcagtacct cagccatcgt
      841 gggtttcggg ggaacagctg gtgtttatgg tatggggctc ttgtggctca ccttcctgaa
      901 catcttggtg gggattttca ttgcattcgt gttcttcgga aagcgtaccc ggaaaatggg
      961 acataatatg ggtgccctga cctttcccga attcctttcc cgacgtttca acagtaaatt
     1021 catccaatac ttctcagggg cagtaatatt cctggggatg cccctctacg catcggtggt
     1081 tctgattggt atggctcggt tcgtggaaac caccctacaa atcgattaca actttgcact
     1141 cgttggaatg gccttaatag tggcagtata cgtgattttt gggggcttac gtggggtgat
     1201 gtacactgac gccctgcagg ggaccatcat gttctttgga atgctgttcc tcctggcttc
     1261 cacctactgg gtgctgggtg gagtggtgga tgccaaccag gccctcacca acctgatcaa
     1321 tatcgtgcca gccagtgcca cggccaaggc caccgccacc ggattcacag gctggacatc
     1381 catgccctca ctgggaagtc ccttctggtg gaccctggta agcaccctca ttttgggagt
     1441 gggtattgga gtgttatccc aaccccaact ggcagtccgt ttcatgaccg ttaaatccaa
     1501 ccgggaactc aaccgggcgg tgctaattgg tggtatattc atcttcatga tgactggaac
     1561 tgcctttgtg gtgggtgccc tgtccaacgt gtatttcttt gacacggtgg ggaaattagc
     1621 catcgcagta gcaggtggta atgctgataa gattataccc accttcattg gaggtgccat
     1681 gcccctgtgg ttcgcctatc tgttcatgat caccctacta tcagcggcca tgtccaccct
     1741 atccgcccag gtgcacgtgc agggaacagc cctgggaaga gacatctacg aaaccgtcac
     1801 cagtaaaact ggtggctctt ctgtgatgat agcgcgagct gggatagcca tagcagtgat
     1861 catcgcggtg atcctggggt tcatcttacc agccaacatc atagccgtgg gaactgccat
     1921 gtggttcagt ataactgcgg catccttcct gtcactgtac atcttcgccc tgttctggaa
     1981 gcgttccacc aaggtagggg ccattgcggg tcttttgact ggtacctttt tcagcatgtt
     2041 ctggatactc ttcggatatc agaaatcagc cgcggctctg ggtatctgcc aggccatcac
     2101 tggtaatgcg gttattttaa ccaccgcgcc ctggcccacc gtagacccca ttgtgattgg
     2161 cctgccattg gccttcgtgg tcactgtggt ggtaagttta ttgaccaaac cacccgccca
     2221 ggaacacatg gacctctgtt ttaaggggat ttaaatttct aatccctctt tttaatattt
     2281 tatattaaaa aaataattac accaattgaa ttaatcttag ttctatcata cggagtttta
     2341 accttatttt gataaattat aacctgaaaa attcagaaat acttgtagaa ataaactttc
     2401 taaaaaaata tatagtatca gtgaaatatt ataaaaacaa atcagtacat aaaaaggtcg
     2461 aggattgaaa aacccgtttt gatggtgatt aatatgagaa taattgaaga aataattgga
     2521 aaagaagttt tagacagctc agcagtggta attggtaaag ttagggatct ggagttgaat
     2581 gtggaaacca accagatcga agccttcatc ctaggaaaag gtggcatttc ggagggactg
     2641 ggactgtcta aaggggaaac agtagtcccc tacgatatga ttaaacagat tggagacaaa
     2701 attctcctta aaaatgcttc agaaactctt taaataggaa ttattttggt gggtgtctta
     2761 gttggagatt tcgggcatct gtcctatctg tggaaaacag gggaagatgt atccctgtca
     2821 actctgcggt agccgggttt gtagtgagtg ttaccatcac cagagagggg tgtgtcgttt
     2881 gtgtcggaga gggctgagga tggattgatg tacatggatg aactgaaaag aaatctttta
     2941 aaactattaa ccacccacca ggtgatccag tttggtaagt tcaccctggc atccggccgg
     3001 gagagtgatt actacgtgga tatgaagaag gccatcaccg atccaggcat cctaggagaa
     3061 attggacaga tgatggctgg aaaaattacc agggaagatg cggacaaaat agctggccca
     3121 gccctgggag cggtgcccat tgccactgct gtctcactgg ccacaggaat acccatgctc
     3181 atgatacgga aatccagaaa aggttacggc acctccaaac tcatagaggg agacctggaa
     3241 gaaggagatc gggtggtggt ggtggaagac gttaccacca caggaaat
//
LOCUS       DAOQVW010000137         3263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_33812_length_3263_cov_3.941397, whole genome shotgun sequence.
ACCESSION   DAOQVW010000137 DAOQVW010000000
VERSION     DAOQVW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33812_length_3263_cov_3.941397"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PKK85_03945"
     CDS             complement(<1..1529)
                     /locus_tag="PKK85_03945"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNS25201.1"
                     /translation="MRILLIHSDYLKYETKNQTSIAEKIDEDRKKGRYDDVLVVFTAV
                     EKEDEEDPQTVIEKAAQEIEEVYAKVKADKIVIYPYAHLSSSLGLPKFAKKVLNELKS
                     ALEDKGYSVDRVPFGWYKSFQVACKGHPLSELSRTITAEPMEKEAEKEEEESQFYVLF
                     QGELHHPKTFHYKNPDLEQLINYELGISKSSGAEPPHVKLMREKNLADYEPSADVGHL
                     RWYPKGRLIRDLLSDYVYRLVTERGAMPVETPIMYDLADDAIREHAEKFGERQYRMGG
                     KKELMLRYACCFGAFRVLSDSFLTWKNLPVGIYELSTYSFRLEKKGEVVGLKRLRGFT
                     MPDLHTVCADLKQSLQEFNQQIYMCQQTGEDLNVNYEVIFRATQEFMDQNREWVYQIA
                     EKIGKPVLLEILPRRKHYWIAKMDFAAIDYLGRPIENPTIQIDVESGERFGITYIDQE
                     EKEHHPIIIHCSPTGSIERVICSLLEKAAVAMEHKPPMLPVWLSPTQLRILPVTQRHL
                     D"
     gene            2072..>3263
                     /locus_tag="PKK85_03950"
     CDS             2072..>3263
                     /locus_tag="PKK85_03950"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNS25202.1"
                     /translation="MRSDTIKKGLQRAPHRSLLRACGLTDSEMQKPFIGVANSFTDIV
                     PGHIHLKKVAESVKLGISNAGGVPFEFNTMAICDGLAMNHEGMRYSLASREIVADTVE
                     SMAQAHSLDGLVLLPTCDKVVPGMLMAAARLDIPSIVVTGGPMLPGEFQGEVVDLINV
                     FEAVGKVSSGKMTLDELDELERCACPGAGSCAGLFTANSMACLTEAMGMSLPYCATTH
                     AVDSQKMWIARESGERIVAMIEENLIPSQIMTPEAFQNAIIIDLALGGSSNTALHIPA
                     IANELREKGVEVTLDLFDELSREIPHITAISPSGQHTMLDLHQAGGIPAVLKVLESKI
                     NQKALTCSGEPLKENIKDASVLDHNVIRPLDQPYHEEGGIAILKGSLAPDGSVVKQGA
                     VDQEM"
BASE COUNT          862 a          745 c          725 g          931 t
ORIGIN      
        1 aagtccaagt ggcgttgggt taccgggagg atgcgtagtt gtgtgggtga taaccagacc
       61 ggtaacatcg gtggtttgtg ctccatcgcg actgcagcct tctcaagaag actgcatata
      121 actcgttcaa tactcccggt ggggctacag tggattatga tgggatgatg ttctttttct
      181 tcctggtcga tgtaggttat tccaaatctt tctccactct ccacatcaat ctgtatggtg
      241 gggttttcaa taggcctacc caggtaatca atggctgcaa aatccatttt agctatccag
      301 taatgtttac gcctaggcaa aatctccagc agtactggtt tacctatttt ttccgcaatt
      361 tggtataccc attcacgatt ttggtccata aactcctggg tcgcccggaa tatgacctca
      421 tagttgacgt taaggtcttc tccggtctgt tggcacatgt atatctgctg gttaaattcc
      481 tgaagggact gcttaaggtc agcgcagacc gtgtgcagat ccggcatggt aaatcccctc
      541 aaccttttaa gacccacaac ttctcctttt ttctccaatc tgaagctgta ggtggaaagt
      601 tcatagatgc ccactggaag atttttccag gttaaaaacg agtcagaaag tacacgaaaa
      661 gctccgaaac aacaagcata tcttagcata agttctttct taccacccat gcggtattgc
      721 cgctccccaa atttctcggc atgttctcgt atggcatcat cagctaaatc gtacattata
      781 ggggtttcta ctggcattgc tcctcgttca gttaccaatc tataaacgta gtctgatagg
      841 agatccctga tgagacgccc ctttggatac cagcgcaaat gacccacatc agcggatggt
      901 tcataatcag ccagattttt ctcgcgcata agctttacat gaggaggttc agccccagac
      961 gacttcgaaa tccccagttc ataattgatc agttgttcta aatctggatt tttgtaatgg
     1021 aaggttttgg ggtggtggag ttccccctgg aatagtacat aaaactggga ttcttcttcc
     1081 tctttttccg cctctttttc cattggctcg gcagtgatgg ttcgggatag ttccgatagg
     1141 ggatgtccct tacatgccac ttggaatgat ttgtaccatc caaagggcac tctatccacc
     1201 gagtagccct tatcttccag ggcagatttt aattcgttca ataccttttt agcaaatttt
     1261 ggtaagccga gggaagaact tagatgtgca taggggtata tgacgatctt atcagccttt
     1321 accttggcat agacttcttc aatctcctgg gcagcttttt cgatgacagt ttgagggtct
     1381 tcctcatctt ccttttccac agcagtgaaa acaaccagaa catcatcgta tcgtcctttt
     1441 ttcctatctt catcaatttt ctccgcaatg ctggtctggt tcttggtttc atatttcaaa
     1501 taatcagagt gaattaaaag tatgcgcatt ataccaccta aaaaatatct aaaataaaag
     1561 tcagtgttga tgttatttat acactacctt gaatttagta ctggcctaaa attatatact
     1621 cccaagatac attctaaata aaacttggac aaattactaa cctgctcctc tttctttccc
     1681 ctatgccaat cactgcccct tgggccccat gatgataatc atatgatgga gattcagaat
     1741 taaactaata aagggggaat tattaaaatg aaccccccct ttgatggata tctgaacaga
     1801 cagttaaagt actaagccag gctttgcgaa gtaagatgta aataaatatt caaccattat
     1861 tctaatagct ggtttaaaca tgtttaagtg tatctatcag tgatttcctt caatattcct
     1921 ggtagccttg tttagcatct gctggaacaa tttgtcctta agctttcctc cttttattag
     1981 atgatcctat tataaaagtc atgtcatcta atgatagaac ataaattgat aagtaaagtc
     2041 accactatta ttcgtaaaat caggtgatgg tatgagaagc gacaccataa aaaagggcct
     2101 gcaaagagct cctcaccgtt ccctcctgag ggcttgtggt ttaacagact ccgaaatgca
     2161 aaaacctttc atcggggttg ccaatagttt caccgacata gtccccgggc atattcatct
     2221 aaaaaaggtt gcagaatctg ttaaattagg gataagtaat gctggaggtg ttccatttga
     2281 gttcaacacc atggccattt gtgatggcct ggccatgaac catgaaggga tgcgctactc
     2341 cttggcgtca cgggagatag tagcggatac tgtggaaagc atggctcaag cacatagctt
     2401 agatgggctg gtgctgctac ccacctgtga taaagtggtg cctgggatgc ttatggctgc
     2461 cgcccgtctg gacataccct ccatcgtagt cacaggtggg cctatgctgc ctggagagtt
     2521 tcagggagaa gttgtagatt taattaacgt gtttgaagca gtgggcaagg tttcttcggg
     2581 gaaaatgacc ctggatgagt tggacgagtt agaacgatgt gcctgcccag gagccggttc
     2641 ctgtgccggt cttttcactg ctaacagcat ggcttgcctc actgaagcca tgggtatgag
     2701 tttgccctac tgtgccacca cccacgccgt ggactcccaa aaaatgtgga tcgcccggga
     2761 gagtggagag cgaatagtgg ccatgattga ggaaaatctt ataccttccc agatcatgac
     2821 tccagaagcc ttccaaaatg ccataattat cgacttagcc ctaggaggtt ccagcaacac
     2881 cgccctccac ataccagcca tcgccaatga actacgagaa aagggagtgg aggttaccct
     2941 ggaccttttc gatgagttaa gccgggaaat accccacatc accgccatca gcccctcagg
     3001 gcagcatacc atgctggact tgcatcaggc tggtggaatt ccagctgtgt tgaaggttct
     3061 ggagtccaag ataaaccaga aggcactgac ctgtagtggt gaacctttga aagagaatat
     3121 taaggatgcc tcagtcctgg atcataacgt gatccgtccc ctggaccagc cctaccatga
     3181 agaaggggga attgccatac taaagggtag tttagcccca gatggttcgg tggttaaaca
     3241 gggcgctgtg gatcaggaga tgc
//
LOCUS       DAOQVW010000138         3252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_34006_length_3252_cov_3.651548, whole genome shotgun sequence.
ACCESSION   DAOQVW010000138 DAOQVW010000000
VERSION     DAOQVW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34006_length_3252_cov_3.651548"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="PKK85_03955"
     CDS             complement(<1..1566)
                     /locus_tag="PKK85_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNS25203.1"
                     /translation="MSDFPTDHQQLVHDINNFIEAKMEPSTLIDGKIIQVYMDKKKVK
                     IALDENFNFYMGSIVLVNHERGVVSKHWGKSIEVDFNDVSHFKEGINVTIDTSLMNII
                     LERLESAVNLIQSDSLSNENKKVLDIILGKDRPVNRCSAVDFVSKNLNESQKDAISRS
                     MGVDDFYLIIGPPGTGKTYVIQELIQHLVNGGQKVLITAWTNVAVDNILQRIIGVDES
                     EIIKVGSLDGTSPLVKRFTLSEKRKKHPYYNDVRVIEEVIKRSKKNITELSSEINYLN
                     QKITELSKKREQINESYQEIIKKQDKYNKLLSELEPGLMVADLRKIENQKLDLEQKSS
                     IYFELAMKIEKFSELDDDLPDPDEFYNLGAEIKKMKLGRLLRRATSPLNQKGYQKFTK
                     KLDDKILAQNKLTNLYNEYWNLKDEIESEYYQIYSTDDHIPIDDALDKEFQLLKIFEE
                     YLSLKKLEIHCELENNRIDLVSGAYQSYLQALNQEAERKKTEVKSIKTQMYIEINKRD
                     TLKKEIKNIRNTLD"
     gene            complement(1566..2282)
                     /locus_tag="PKK85_03960"
     CDS             complement(1566..2282)
                     /locus_tag="PKK85_03960"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNS25204.1"
                     /translation="MEISDNKRDLIETIREYRKSKEYEELISEENKMLKDDNQELKNI
                     IEDLSSEIIEYHNLLDEVNELKDRTRLLGLENEKLKNQCGVIVTTPRSTSSVIAEIRD
                     NLSKAKKEVLVCSPWITYLLEEFNQFKRGVTLKVVINFRKEDIESGITDLDKIRVLRR
                     LGAQIRYNNDLHAKMIFIDGTTAIISSANLTKRGLSVNYEAGVILKNNEHVQNALKFF
                     KGVWEESEPLNEDMIREMSR"
     gene            complement(2310..2783)
                     /locus_tag="PKK85_03965"
     CDS             complement(2310..2783)
                     /locus_tag="PKK85_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNS25205.1"
                     /translation="MFCKNCGHKNDTDAEFCVNCGLSLLKNKMGVNNLNKVLFLSCVV
                     LIGSLAVVAGVLLSNPDKTINSIENNTTSTLVSTKTPTQVNKIKNTTSEKKSTSNGIR
                     GVGNNGIKYWIPANHQTCPSCGGPLGYYYIDKVDLSTGEEYTGEYFGCLKGNGII"
     gene            complement(2796..>3252)
                     /locus_tag="PKK85_03970"
     CDS             complement(2796..>3252)
                     /locus_tag="PKK85_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25206.1"
                     /translation="GMATSTKVLIIACIVLVAGLGLAIGLLWQTNSGTPVSVSQTGEQ
                     VTYKASWHEVTSFTGTYNDYRSFQTKGKRFKVVMSATPILNYDTNSMSVDISNSNSIL
                     SSGSLDWGATDALTTKEKTIELTGPPGTYWINIYAMELESWTVKIYDYY"
BASE COUNT          878 a          651 c          519 g         1204 t
ORIGIN      
        1 atccagggta tttcggatat ttttaatctc ttttttcaat gtatcccgtt tatttatctc
       61 aatatacatc tgggtcttta tggacttcac ttcggtcttt tttctttctg cttcttgatt
      121 taatgcttgg aggtaggatt ggtaggcccc tgaaactaga tcaattcggt tattctcaag
      181 ttcacaatgg atttctaatt tcttcaagga taggtattcc tcgaagatct ttaaaagctg
      241 gaactcctta tcaagtgcat cgtcaatagg gatatgatca tctgtggagt atatctggta
      301 atattctgat tctatctcat cctttaaatt ccagtattca ttgtaaaggt tggttagttt
      361 gttttgagcc aggattttat catccagttt tttggtgaac ttttggtatc ctttctgatt
      421 tagaggagat gtggcacgtc ttaaaagtct gcctagtttc atctttttta tctcagcacc
      481 taagttgtag aattcatctg ggtcgggcag atcatcatca agttcactaa atttttcaat
      541 cttcattgct aattcaaaat aaatagagga cttctgttcc aagtccaatt tctgattttc
      601 tatctttcta agatcagcaa ccattaaacc aggctcaagt tcacttaata atttattata
      661 tttatcctgt tttttgataa tttcttgata tgattcattt atctgttccc tttttttgct
      721 tagttcagtg attttctggt ttaaatagtt tatctctgat gataattcag ttatattctt
      781 tttactcctc tttataactt cttcaattac tcgcacatca ttgtagtagg ggtgcttttt
      841 tcttttttca cttagagtaa aacgtttcac taggggactc gttccatcta aggatcccac
      901 ttttatgatt tcactttcat ccacaccaat gattcgttgc aatatgttat ccactgctac
      961 gtttgtccat gctgtgatca gcaccttctg tccgccatta actaagtgct gaattaattc
     1021 ttgtataaca taggtctttc cagtccctgg ggggcctatt attaaataaa aatcatcaac
     1081 acccatagaa cgggatattg catctttttg gctctcatta agatttttag aaacaaaatc
     1141 tactgcactg catctattaa ctggccggtc cttgcccagg attatatcta gaactttctt
     1201 attttcatta gatagggaat cagattgtat aagattcaca gcagactcaa ggcgttctaa
     1261 gatgatgttc atcagactag tatcaatggt aacatttatt ccttctttaa aatgagaaac
     1321 atcattaaaa tctacttcaa tagatttacc ccaatgttta gacacaacac ccctttcatg
     1381 attcactaaa acaatggaac ccatatagaa attaaaattt tcatctaacg ctatttttac
     1441 cttcttttta tccatataca cttggattat tttaccatcg attagggtgg atggttccat
     1501 tttagcttca atgaaattat ttatatcatg gaccaattgc tgatggtcag tgggaaaatc
     1561 actcattatc tgctcatctc cctgatcata tcctcattta aaggttcact ctcctcccac
     1621 acacctttaa agaatttcag ggcattctgg acatgttcat tgttttttaa gataactccc
     1681 gcttcatagt taacagaaag ccccctctta gttagattgg ctgaggagat aatggcggta
     1741 gttccgtcga tgaagatcat cttggcgtgg aggtcgttgt tgtacctgat ctgcgcccct
     1801 aaccttctca ggactctaat tttgtctagg tcagtgatcc ctgattcaat gtcttctttt
     1861 ctaaaattaa taactacttt gagggtcacg cctcttttga actgattaaa ctcttctaat
     1921 aggtaagtga tccagggtga acatactaaa acctcttttt tggccttgga taaattatcc
     1981 ctaatttcag caatcactga actggtagaa cgtggagtag taactatcac tccacactga
     2041 ttttttaatt tctcgttttc tagtcctaaa agcctagttc tatcttttaa ttcatttacc
     2101 tcgtctagaa gattatgata ttcgattatt tcactgctta agtcttctat tatattttta
     2161 agttcttgat tatcatcttt taacatctta ttctcttcac ttattaactc ttcatattcc
     2221 ttagattttc tgtattccct gattgtttct ataagatctc gtttattatc agatatttcc
     2281 atataatatc cccattgata aatattcttt cagattatcc cattaccctt caaacatccg
     2341 aagtattctc ccgtatactc ttcaccagtt gataaatcaa ctttatctat atagtaatat
     2401 cctaatggac ctccacaact agggcaagtc tggtgatttg ctggtatcca gtactttatc
     2461 ccattgtttc ccacgcctct aattccattg gatgttgatt ttttttctga agttgtgttc
     2521 ttgattttat tgacttgagt aggtgttttg gttgaaacaa gtgtagaggt agtattattt
     2581 tctatggagt tgattgtttt atctggatta gatagtagta ctccagcaac aactgctaaa
     2641 ctaccaatta acactacaca agataggaac aaaactttgt ttaggttgtt aacgcccatt
     2701 ttattcttaa gaagagatag accacaatta acacaaaact ctgcatcagt gtcatttttg
     2761 tggccacaat tcttacaaaa caattcaatc acctactaat aatagtcata aatcttcaca
     2821 gtccagcttt ccagttccat agcgtagata ttaatccagt aagttccagg tggtccggtg
     2881 agttcgatgg ttttttcctt agttgttaaa gcatcagtgg ctccccagtc taaactacct
     2941 gacgagagaa tgctgttact gttgcttata tccacgctca tggagttggt gtcgtaattt
     3001 aagatggggg tggcggacat caccacttta aagcgcttcc ccttggtctg gaaagatctg
     3061 taatcattat aagtcccagt aaaactggtt acttcatgcc aactcgcttt ataggtgact
     3121 tgttctccag tttggctaac tgatacagga gtaccggaat ttgtttgcca cagaaggccg
     3181 attgctaatc ctaaacccgc taccagaaca atacaagcaa taattaaaac cttggtagat
     3241 gtagccatac ct
//
LOCUS       DAOQVW010000139         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_34233_length_3238_cov_3.259189, whole genome shotgun sequence.
ACCESSION   DAOQVW010000139 DAOQVW010000000
VERSION     DAOQVW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34233_length_3238_cov_3.259189"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1289..3199
                     /locus_tag="PKK85_03975"
     CDS             1289..3199
                     /locus_tag="PKK85_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036094 - small molecule binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3H domain-containing protein"
                     /protein_id="HNS25207.1"
                     /translation="MQNPIPPNPVVPSIPASILRYALLGVGLLLTYGFIGSIVIMRLD
                     PVNALYFAVITTATVGYGDISPSNPLQKLFVVTLVLGGASLLAYAFTLAIAVVSMTFE
                     DITSGARERRRIASLNNHFILCGYGRVGSAVYRELKKRNQQALIIERDKNVVEKELWV
                     DPEVMAIHGDATEESLMYQARVEDARGVIITTGDDVDNLFITFTARELNSEVWIVARA
                     SKEENIKRLSRSGADKVISPETSGASDIYFAAIEPTIMKITVMHDVSDIRKESGIILK
                     HGCTLENIEYHLPEFKEPLARTVGLSDPERLERFLKSLERDKSRKKSLEKIYESVNGI
                     HSHWISGPDKHSLEKVAKELKNEGILLGIDLNEEEIKEKARKYGRLVEVVIKPEIKIT
                     EMHGVSDIKEEAEIILNHRCTMEDIEYYLPGFREPLTRKINLSEMDEMDRFLDGLQHD
                     SVKKENLERLYTLSGGGIHSHRVSGPDTKTLGLVEKKLKEKGFLLGVNFSKDEITDKI
                     QKFGRVSEMLLKHDVGQIDDKKIIIRLGGRILDSKHYLPGVHQVVTRKLYLKSMEDLE
                     RCEKEYQKPDAKRSLQVLDEISRHIHSHTVSAPDLKVIKDIEKALIKEGILLGVDVPE
                     NDVWEFVESAEA"
BASE COUNT         1064 a          568 c          650 g          956 t
ORIGIN      
        1 cggcattggc atccacaacc atcatgtcct tgggagtcat gttatcacaa gtttattcta
       61 tgtaactaag aagtaattct tacaatcatg gagtcaatac ccattcattg gtgaggataa
      121 tgggctccaa tttaaatttg attatgacaa tattaaaaac ttactcgata ccgatgagat
      181 gtttcaagaa ttcttaatag tcaaaagcgt gtaccgagca ccattttaga atatgaaaaa
      241 gaattgtctg gctattggat taacccctac tcagtttagt ggaaaggctg aaatcgaaga
      301 agatgctgga attaaattaa aaaaatagaa tttcgttcaa ttcaccaatc ctaaagaaat
      361 aagggtttgg tggtggaaaa atcattgaaa agagtacttt cttaaaaaaa tcaataaagc
      421 attcgcacca ttacataagc cacgtatttc tttagaaaga tttccgcctc ctgattagtc
      481 ccatatacgt aaagtatcca acttgggtta atccacttac tattactgga agatccaaaa
      541 tactctgcag tgaaaaaaat ttatctatac gccgtcttct ctctattcgg gctttatacc
      601 aatgattata tctaaataac gcacgtttcc actggagcca ttagtatact tctaagattg
      661 ttatgatgta aatcctcagc gttttctcaa agaacactat tgcgccccat aacccttcac
      721 tttgaattga attcaatcca tccccaaatc ttcttatctt tcaactattt tgaagtagaa
      781 atccatcgta gtccaatttc acttaactta acatcatcgc tatcattata attgcgccga
      841 aagtgcacat tacagtaaca aatagccaaa atagaaaact taataagttt cttatcattt
      901 tttttcaacc atggtttaat ttaaagaaaa tcatttatct gattactagt gtaaatcttg
      961 ttaattctca ttttaaattg atattatccc atagaaactc ctgcgaacca ttgtatccat
     1021 tttttaatag tctttaaact gtttaattta tccttgccat atctttctat atggaactgg
     1081 aagagataaa attaataggt cttagaagca tataattgcg tgaaattatc taaattagat
     1141 ggagagatta taaaaatgat cctgaaagtt atatcatcca gtagtgtgga atgtcctaac
     1201 aagtatgaac tatggattat aaaaggtgat tttctgatct aaggatttta atcctatttt
     1261 tcgctaattt tatcttaggt ggtgtaagtt gcaaaatcca atacctccca atccggtagt
     1321 tccttctatt cctgcctcta tccttagata tgctcttctg ggtgtgggtc tacttttaac
     1381 ctatggattt attggatcca ttgtcattat gcgacttgat cccgttaatg ccctgtattt
     1441 tgcagttata actacagcaa cagtgggata cggagatata agcccttcaa atcctcttca
     1501 aaagctattt gtagtaactc tggttctagg aggagctagt ttattagctt atgcatttac
     1561 acttgccata gcggtggttt ccatgacgtt tgaagatata acatccgggg ctcgtgaaag
     1621 acgacgaatt gcctccttaa acaatcattt catattgtgc ggctacggtc gtgtagggag
     1681 cgccgtgtat cgggagctca agaaaagaaa tcagcaagcc ctaattattg aaagggacaa
     1741 aaatgtagtt gaaaaagagt tgtgggtcga tcctgaggtt atggccattc atggcgatgc
     1801 cactgaagag tctttaatgt accaggctag agtggaagat gcaagaggag tgatcataac
     1861 cactggtgac gatgttgaca atctattcat cactttcacc gcacgagaac ttaattctga
     1921 agtttggata gtggccaggg ctagtaaaga agagaatatt aaaagattat cacgctcagg
     1981 agccgataag gtaatatcac ccgaaaccag tggagcatct gatatatact tcgcagccat
     2041 cgagcccacc atcatgaaga taactgtgat gcacgatgtg tcagatattc gtaaggagtc
     2101 gggaataatc ctgaagcacg ggtgcaccct ggagaatatt gaataccatc tcccagaatt
     2161 taaggaaccc ctggcccgaa cggtgggttt atcagatccg gaacggttag aacgtttttt
     2221 aaaaagttta gaacgtgata aatcacgtaa aaagtctttg gagaagatat atgaatcagt
     2281 taatggtatc cattcccact ggatttcggg gccagataag cacagcctag aaaaggtagc
     2341 taaagagctt aaaaatgaag gtatccttct ggggattgat cttaatgaag aagagataaa
     2401 agaaaaagct cgaaaatatg gaaggctggt ggaagtagtt ataaaaccag agatcaagat
     2461 taccgagatg catggagtat ccgatataaa agaagaagct gagataatct taaatcatcg
     2521 atgcaccatg gaagacattg aatattatct accaggattc agagagccct taaccaggaa
     2581 gatcaatctt tctgaaatgg atgaaatgga ccggttcctg gatggactgc agcatgattc
     2641 agttaagaag gagaacttag aacgtcttta caccctttca ggcggaggca tacattctca
     2701 tcgtgtttct gggccggaca ctaaaactct gggtttggtg gagaaaaaat tgaaagagaa
     2761 aggttttctt ttaggagtta actttagcaa ggatgaaata actgataaaa tccagaaatt
     2821 cgggagagtg tcagaaatgc tcttaaaaca tgacgtggga cagattgatg ataaaaaaat
     2881 cattattagg cttggtggac gcattcttga ttcaaaacat tatttaccag gtgtacatca
     2941 agtggtgacc cgtaaactgt atttgaagtc catggaggat ctggaaaggt gtgaaaagga
     3001 gtaccagaaa cctgatgcca aaagatcctt acaggtactt gacgagatct ccaggcacat
     3061 acattcacat accgtttctg caccagattt aaaggtgatt aaagatattg aaaaggcttt
     3121 gattaaggaa ggcatactcc ttggtgtgga tgttcctgaa aatgatgtct gggagtttgt
     3181 agagagtgct gaagcatgat ggttttattg tagataaaaa agcaaataaa catcagat
//
LOCUS       DAOQVW010000140         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_34260_length_3237_cov_3.131992, whole genome shotgun sequence.
ACCESSION   DAOQVW010000140 DAOQVW010000000
VERSION     DAOQVW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34260_length_3237_cov_3.131992"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PKK85_03980"
     CDS             complement(<1..1241)
                     /locus_tag="PKK85_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNS25208.1"
                     /translation="MDRTSTRLLKNSFKINQKNVFGVLGVCGIVGNLAARVLMDHEYE
                     VLGSDLESAESCQYLYTLEDYNLPLYLSGHAEEFFNKSDYFVPPPSLPQDSDTFHKLE
                     SSGKIIKVDDIIDAIEPGKPVVCITGTNGKTTTTHLLKHIARIAGLKTTEHGFKTLQG
                     NVDYIPPLQARLKADIAVLETGTEGTPGDLKFTVEKCNPAYGILTNINPDHLSQDADF
                     LSYARIKGELLEELQDGVAVINCDDPTIWGLLKEVNYHGKVITFGVEDHPSRISSKKC
                     WCGEELLLGETISGVGYYRCQCGLERPQPDYRATDITPNKFLLHTPQGIHTINMSIMG
                     LHNVYNALGSIVVAREFLEISMNDIKKGLETFKGVPGRLEHIKSLPNTDFIVDYAHNP
                     SGVETVLQELKKIYSKVAVVIT"
     gene            complement(1562..2989)
                     /locus_tag="PKK85_03985"
     CDS             complement(1562..2989)
                     /locus_tag="PKK85_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNS25209.1"
                     /translation="MKEITTAYLASHVQGKLLGKNRKITGVFNILNDAKNGDVVIRHW
                     IDGSGVDMAASKGVTCIVTQEARGSAIYKVKKYDMCLIITEKIEYANAFALRWAIKTF
                     APESIRVVITGTNGKSTTSHMIHKILQVAGFKSHTNTDSLSEFNTLIDPMVAKQISET
                     KNLEILVLEVSEVQGWEERIMSDHAFLMTQAIQPQILVFTNVSMDHINLVSSIEEAYQ
                     EISGALRNFNGKTVVLNADDPLIMNMKDLVPSEAEIITFGHGGQVEMMEEGLYLMGDI
                     LLKQEELPFHSPHFLQNTMAAIAACKALNIDNEVIKKGVKSYHALKRRFSILNRKPLI
                     VDDFAHNPDGIKATIKSVAELSKNQLYIVCSIRGSRGKDINEINAMSIAQSLKGLNYT
                     LVITSSQDVVDKANWVQEDEKKVFIDVLKKEGIQYSHYSQLRIALFEVVNMSSSEDTI
                     LLIGAQGMDPASDILEKFKEEGIIK"
     gene            complement(3000..>3237)
                     /locus_tag="PKK85_03990"
     CDS             complement(3000..>3237)
                     /locus_tag="PKK85_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNS25210.1"
                     /translation="ITAGFLGDVIYLAVLALAIPIVSVMISLFHRVHLIKLPVEPLHH
                     TLHYKGLSEKKIVAIYWGVTFLICSVAIIYQFLL"
BASE COUNT          897 a          669 c          635 g         1036 t
ORIGIN      
        1 gttataacta cagctacttt ggagtagatc ttttttagct cctggagaac ggtttctacc
       61 ccagagggat tgtgggcata atccacaatg aaatcagtgt tgggcaatga ttttatatgc
      121 tcaagacgac ctggcacacc cttaaatgtt tccagacctt ttttgatgtc attcattgat
      181 atttctaaaa actcccgggc aactactatg ctccccaggg cattgtaaac attgtgcaac
      241 cccattatac tcatatttat ggtatggata ccctgaggcg tgtgtagtag gaatttgtta
      301 ggagtaatgt cggtggcacg atagtctggc tggggacgtt ccaagccaca ttggcagcga
      361 tagtaaccaa ccccagatat ggtttctccc agcagtaatt cttctccaca ccaacatttt
      421 ttactggaaa tcctggaggg atgatcttcc actccaaatg tgataacctt accatggtag
      481 tttacttctt ttagaagtcc ccatatggtg ggatcatcgc agttaatgac agccactcca
      541 tcctgaagtt cttccagaag ctcccccttt atacgtgcat agcttaaaaa atctgcatct
      601 tggctgagat ggtcaggatt tatgttggtc aagattccat aggctggatt acatttctcc
      661 actgtgaatt taagatcacc aggagttcct tcagttccag tctccaacac tgcaatatca
      721 gcctttaaac gggcttgtaa ggggggaata tagtctacat tgccttgaag tgtcttaaaa
      781 ccatgttcgg tagttttaag tcctgcgatt cttgctatgt gtttcagtag gtgggtggtg
      841 gtggttttac cattggttcc agtaatgcat acaactggtt ttcctggttc tatcgcatca
      901 atgatatcat caacttttat gatctttccc gaactttcca gcttatgaaa ggtatctgag
      961 tcctggggga ggctgggggg aggtacaaag tagtctgatt tattaaaaaa ctcttcagca
     1021 tgtccagata ggtatagggg taggttatag tcttctaaag tatataggta ctgacaactc
     1081 tcagcagatt ccagatcact tcccaaaact tcatattcat gatccatcaa aaccctggct
     1141 gccaagttcc caactattcc acatactcct agaactccga aaacattttt ctggttgatc
     1201 ttaaatgagt ttttcaggag tcttgtactg gttctatcca tatcttcaac aactctcaaa
     1261 aatacatgcc tttcgataat ataattaagt tttaaacttt aatcagtcta tttaacggtt
     1321 gacgttacaa ccactttata ttcctgataa caactccaaa gttgatttgt actggttgat
     1381 tattcatctg tgactgggtc ggtgataata ggggtggtaa taatatatat aatattagat
     1441 tctgggatta taacgcgtct aatctctttt ataaatgttt attaaggaaa tttatagaaa
     1501 atcaatgaat gtacgggtgt aacagaagct aattattctc atcaaattat tgatttttat
     1561 actatttaat tatgccttct tctttaaatt tctctaaaat atcagatgct gggtccatac
     1621 cctgggcacc tatcaataat attgtatcct ctgaactgga catattaacg acttcaaata
     1681 gagcaatgcg taattgggaa tagtgactgt attggatacc ttcctttttc agtacatcta
     1741 taaatacctt tttctcatct tcctgtaccc agttggcctt gtccaccaca tcctggctac
     1801 tggttataac aagggtgtaa ttgagacctt taagagactg ggcaatagac atggcattaa
     1861 tctcgtttat atcctttcca cgagatcctc ttatggagca tacgatgtac aactggtttt
     1921 tggatagttc agccacactt tttatggtgg ctttaatacc atctgggttg tgagcaaaat
     1981 catctacaat aaggggtttc ctatttaata tggaaaatct tcttttaagg gcatgataag
     2041 acttaactcc tttttttata acctcattat caatatttaa agctttacaa gcagcaatag
     2101 ctgccatggt attctggagg aagtggggac tatggaatgg tagttcctct tgttttaaca
     2161 aaatatcccc cattaaatat aatccctcct ccatcatctc cacttggccg ccatgcccaa
     2221 aagttataat ctccgcttca gaaggcacta aatccttcat attcataatc agggggtcat
     2281 ctgcattcaa aaccactgtt tttccattaa aattccttaa tgcacctgaa atctcctggt
     2341 aagcttcttc aatggagctc accaggttga tatggtccat agacacattg gtgaatacta
     2401 gaatttgggg ctggatggcc tgagtcatta aaaatgcatg gtccgacata atccgttctt
     2461 cccatccctg cacttcagaa acctccaaaa ccaggatctc cagattcttg gtttcggaga
     2521 tctgtttggc gaccatagga tcaataaggg tgttgaactc tgatagggaa tcagtattgg
     2581 tgtgactttt aaatcctgcc acctggagta tcttatgaat catgtgactg gtggtggact
     2641 ttccattggt cccggtaatt accacccgta tggattcagg tgcgaaagtc tttatagccc
     2701 agcggagagc gaaggcattg gcatattcga tcttttcagt aataatgagg cacatatcat
     2761 attttttaac cttataaatg gcggatccac gggcttcctg ggtcactatg catgtcacgc
     2821 ctttactagc agccatatcc actcctgagc catctatcca atgtctgatt actacatcac
     2881 cattttttgc atcgttaagg atgttgaaaa caccggtaat cttacgattt ttgcctagaa
     2941 gttttccctg aacatgggat gccaagtagg cggtggtaat ttctttcata attcaacctt
     3001 caaagaagaa attggtaaat gatggccaca gaacagatga ggaaggtaac accccagtaa
     3061 atggccacta ttttcttttc tgagagacct ttatagtgca gagtatggtg tagaggttct
     3121 acaggtagtt taattagatg aactcggtgg aagagactta tcatcacgga tacgatgggt
     3181 atggccaggg ccagcactgc caagtatatc acatcaccta gaaatccggc tgtaatg
//
LOCUS       DAOQVW010000141         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_34322_length_3233_cov_3.315293, whole genome shotgun sequence.
ACCESSION   DAOQVW010000141 DAOQVW010000000
VERSION     DAOQVW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34322_length_3233_cov_3.315293"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKK85_03995"
     CDS             <1..271
                     /locus_tag="PKK85_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNS25211.1"
                     /translation="PSIPRALNSLAKKGVRKIIAMPIFLAHGLHTKKDIPYMLGLGEG
                     RKDASYYHMEQEPIEFDGEIIYIEPLGADVRIADIIEKRVEDALK"
     gene            complement(360..953)
                     /locus_tag="PKK85_04000"
     CDS             complement(360..953)
                     /locus_tag="PKK85_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNS25212.1"
                     /translation="MEESKDILPFSEVTKFHGHVCPGTAIGYRAGEIAIKELGSSRAE
                     DEELLAIVENDSCSVAGCTLGKGNLIFKDHGKHVYTFINRDSGDALRLSLKKGLDEIG
                     SDFDDLRNKAFSESATPEDKLEFEKEKDRVTKKIMAMPAEEIFKIEQVDIDIPGEARL
                     FSSVRCAKCGELMAEHRARVENGALVCIPCFEDYSRN"
     gene            1420..2163
                     /locus_tag="PKK85_04005"
     CDS             1420..2163
                     /locus_tag="PKK85_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine tRNA-ribosyltransferase"
                     /protein_id="HNS25213.1"
                     /translation="MLEITLHNGPARLGKWNNLITPTILDSEEVAVVDDQAMPYEVPR
                     ALAEWSVEETILQAQDGDRNNMAVIHGAKYLDLRLECATELEKLGYQNFLVANMDKLL
                     KRPRDLVGMMVTIRETVNPNSSLYVSYCNPNHIPLLSYLGVDLFGDASCGYHALLGVM
                     LTPDGNYPVDEYEICPAENCDTSYSGLLKYNKHSMDFVIREVKAHMKNGTLRNLVEMR
                     CASSPETMAVLRILDADHSDFLLKYTGLY"
     gene            2209..2376
                     /locus_tag="PKK85_04010"
     CDS             2209..2376
                     /locus_tag="PKK85_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25214.1"
                     /translation="MLDQYLCPNINCNHLKKGRKGKSCPECGEPFKKVGIIEGTNLIQ
                     QKKKSSANQLM"
     gene            complement(2397..2972)
                     /locus_tag="PKK85_04015"
     CDS             complement(2397..2972)
                     /locus_tag="PKK85_04015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS25215.1"
                     /translation="MSGKKLHRHGGKSSEAVLNAQRVLRSINLCEGDVFMDAGCGDGF
                     ISRAASRMVGAEGEVLAVDQHSPSLDILREKVREEKIKNLKVLEADLTRKLSIPDETV
                     DLCLMANVLHGFFHNEELDQAIPELLRILKPGARLAIVEFKKIEGMPGPPFAHRITAE
                     DVRGILESRGFQFQEEMEVGPYHYAVVLAKK"
     gene            complement(3024..>3233)
                     /locus_tag="PKK85_04020"
     CDS             complement(3024..>3233)
                     /locus_tag="PKK85_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS25216.1"
                     /translation="LGEVMDQQFQVYDILPWHHVLNALRSILTFGGGWNDIAQQVGWA
                     VLLTTILFMGGVFIFARTRLRPDKQ"
BASE COUNT          865 a          766 c          720 g          882 t
ORIGIN      
        1 gcctagcata ccccgtgcac ttaactcact ggccaaaaag ggtgttagaa aaataattgc
       61 tatgcccatt tttttagccc atggtcttca caccaaaaag gatattccct acatgcttgg
      121 cctgggagag ggacgcaaag acgcaagtta ctaccatatg gagcaagaac caattgaatt
      181 tgatggtgaa attatctaca ttgaacccct tggagctgac gtgaggattg cagatataat
      241 tgaaaaaaga gtggaagacg cccttaaata gatttgggca tcattctata tttactgcgc
      301 ttaacacaac aaactcccac cataattaat ggtaaatgat cttctgaacc acacccccct
      361 taattccgtg aataatcctc aaaacagggg atgcacacca gggcaccgtt ctctactcgg
      421 gcccggtgtt cagccatcag ttctccacat tttgcacatc taacagaact gaacaatcgg
      481 gcctccccgg gtatgtctat atccacctgt tcgattttga atatttcttc ggcaggcatg
      541 gccatgatct ttttcgtgac cctgtctttc tctttttcaa actccaattt atcttctgga
      601 gtagctgatt ctgaaaatgc cttatttcga agatcatcaa aatcagatcc tatttcatcc
      661 aggcctttct ttaaagataa acgtagagca tctcctgagt ccctattgat aaaagtgtaa
      721 acatgttttc catggtcctt gaatattaaa ttgcccttgc ccagggtaca gcccgccaca
      781 ctacagctat cattctcaac gatggccagg agctcctcat cttctgctcg ggatgaacca
      841 agctccttta tagcaatttc ccctgcacgg tagcctatgg cggttccagg acagacatgg
      901 ccatggaatt tagttacttc tgaaaagggt agtatatctt tagattcctc cattatttca
      961 cctgttttca tattcctaag aatcataagg aatttcttaa taccatgaat tataccttgt
     1021 gggggatata taacattggc tgatattatt aagctttagc tactggtatt gtgaattcat
     1081 acagtaacag tgggttacgt gaataatttg ggagaatagg tttataaaat taaaaaaaat
     1141 tgttaaagga ggattactgt ggtccattcc aggaaacttc gactaaaaag gttccaaaat
     1201 tgtggtgggc ctgggccagg tcctatgttc tgggccatgg agatggatct ttgccaggcc
     1261 ctacttactc ctgggcaggg caaggatggc tacacttaaa acagatggtg tcactataat
     1321 attctttgag taacaaaaat ctattgtata aattgtaccc ggaaaggcag ggtacttaac
     1381 aatattacac catttcattg ccagggagtt gattgatgta tgctggagat aactctacac
     1441 aatggccctg cccggctggg caagtggaac aaccttatca cccccaccat tctagactct
     1501 gaggaggtag ctgtggtgga tgaccaggcc atgccctatg aagtgccccg ggccctggcg
     1561 gagtggtccg tggaggaaac catcctccaa gcccaggatg gtgatcggaa taatatggct
     1621 gttatccacg gggccaagta cttggatctt cgcctggagt gtgccacgga actggaaaag
     1681 ttggggtatc agaatttcct ggtggccaac atggataaac tcctgaaaag gccacgtgac
     1741 ctggtgggga tgatggtgac cataagggag actgttaatc ccaattcctc gttatacgtg
     1801 tcctattgta atccgaacca cattccactc ctctcttatc tgggtgtgga tcttttcgga
     1861 gatgcctcct gcggatacca cgccctcttg ggggtgatgc tcaccccaga tggtaattat
     1921 cctgtggatg aatacgagat ctgcccggcg gagaactgtg ataccagcta ctctgggctt
     1981 ttaaagtaca acaaacacag tatggacttc gtcatccggg aagtgaaggc tcatatgaag
     2041 aatggaacat tacggaacct agtggagatg aggtgtgctt cctcccccga gaccatggct
     2101 gtgctccgga tactggatgc ggatcattct gatttcctgt tgaagtacac cggcttgtat
     2161 tgaattagta aagattgtgt aaaaacaagt tacgaggcct gaaaaaaaat gcttgaccag
     2221 tacctatgcc ccaacatcaa ttgcaaccat ctgaaaaaag ggagaaaggg aaaaagctgc
     2281 ccagaatgtg gtgaaccctt caagaaggtg ggaatcatag aaggcaccaa ccttatacag
     2341 cagaagaaaa aaagttcggc taatcagtta atgtaagggg gaataggatc ctatcctcat
     2401 tttttagcca aaaccacggc gtagtggtag ggacctactt ccatctcctc ctggaattgg
     2461 aagcccctgc tttccaggat acctctaaca tcctcggctg tgatacggtg ggcaaatggt
     2521 ggtccaggca tgccctctat ctttttaaac tctacgatgg ccagcctggc acctggcttt
     2581 aaaattctta aaagctctgg gatggcctgg tccagttctt cgttgtggaa gaagccgtgt
     2641 aatacgttgg ccataaggca cagatccact gtttcgtcgg gtattgataa cttccgggtt
     2701 agatcagctt ccaacacttt caagttcttt attttctctt cgcgaacctt ttcccgcagt
     2761 atatccaggg aggggctgtg ctggtccact gccagcactt caccctcggc acccaccatc
     2821 ctggacgcgg cccgggatat gaagccatca ccacaaccag cgtccatgaa cacgtccccc
     2881 tcacataggt taatggatct taaaactcgc tgggcattaa gcactgcttc actggacttt
     2941 ccaccgtgtc ggtgcagttt ttttccactc ataatgatac ctcttacaat tccagatgac
     3001 tttaaccttg atgtcggttt gttttattgt ttatctggcc ttagccttgt tctggcgaag
     3061 atgaacactc cccccatgaa aaggatggtg gttaagagca cggcccatcc cacctgctgt
     3121 gctatgtcat tccatcctcc cccaaaggtg agaatgcttc gcaaagcatt tagtacgtgg
     3181 tgccagggta aaatatcgta tacctggaac tgttgatcca tcacctcacc cag
//
LOCUS       DAOQVW010000142         3219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_34555_length_3219_cov_3.209861, whole genome shotgun sequence.
ACCESSION   DAOQVW010000142 DAOQVW010000000
VERSION     DAOQVW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34555_length_3219_cov_3.209861"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKK85_04025"
     CDS             complement(<1..666)
                     /locus_tag="PKK85_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS25217.1"
                     /translation="MMETKKIYWMIKKDLLVLWRHKPRLISIFIFPILMIALFGYGMG
                     GSIENIPVVVVKQSQGMVTDSTLTHIKGMDLYEVKDIISDPDRARQMVETGVVKAAII
                     LPPDYEDLNSGNPKSVVVYVDSSDQMATQALVPATQALFSQISTQIGLQKLQTMTAQS
                     SRVKVQSQGVNTTTGSSLSSINYQNIMNSINFQINKIYGDIKYIDFLVPAILAMTVMM
                     GAMM"
     gene            complement(668..1747)
                     /locus_tag="PKK85_04030"
     CDS             complement(668..1747)
                     /locus_tag="PKK85_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS25218.1"
                     /translation="MKYAIETFDLTKRYGNFVAVDALDLKVKNKSIFGFLGPNGAGKT
                     TTIKMLTCLIPPTSGTAQVSGFDIIKSPDEVRQKIGMVPQLVSLYGDLTARENAELCA
                     DYYGMPRDLKEQRINELMELVDIKYAENKLIRQMSGGQRQKVSVVASLVHQPDILFLD
                     EPTIGLDPTTKSVLWELIDELNQKGHTIILCSHDMYEVDMLCDHIGIINLGKLAAFDT
                     PQGLKDSILTKNDNKASNVGEIVRELEESEIISHPSFSRLKTIVNKSEIDNAREMSLM
                     ISNSDSELIKRLYQIPSVLEIETHPSGRLTFKLANTENAVTQVISVVMETGGNITSIS
                     TKDPSLEDVFTKVTAKKVKKEEAGE"
     gene            complement(1760..2230)
                     /locus_tag="PKK85_04035"
     CDS             complement(1760..2230)
                     /locus_tag="PKK85_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNS25219.1"
                     /translation="MPGNNLNSKDNSNKNSVKNKKKDPCAENIKNLAEADKKIIGGLM
                     KAFGKIMILWLISKKRQHGYEIMTQIHKSSPYNKKMPSASMIYPVLHDLEKKGMICGA
                     WEKQGKRRVKYYAITADGEKNLENIRKIAQCNREHDNINIWDEFMDDLFGYKTR"
     gene            2551..>3219
                     /locus_tag="PKK85_04040"
     CDS             2551..>3219
                     /locus_tag="PKK85_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNS25220.1"
                     /translation="MRIGFSTLVLFAKPFEDWLERATSDGFNMIEILCEGPYWPRILL
                     SECDGLDIFSSYDIDLYLHAPTIDLNPASLNPGIREETIKQLYETIDLASEIGAKAIT
                     THPGLIHRLEDRIRNMALYYSIETLKDANIYAEDFGIIFSVENMPHRYAYFCNNPQEH
                     TYFTRECGSHATLDLGHANTSGELEKFLDMPRIHYYHVSDNNGEKDQHLSLGEGTLDL
                     GVLNG"
BASE COUNT          877 a          645 c          595 g         1102 t
ORIGIN      
        1 catcatagct cccatcataa ccgtcatagc tagaatggcc ggcactaaaa agtcaatgta
       61 tttgatatct ccatagatct tgttgatctg gaagttgatg gagttcatta tgttctggta
      121 attgatactt gacagtgatg aaccggtggt agtgttaact ccctgagact gcactttcac
      181 cctggaagac tgggcagtca tggtttgaag tttctgcaat ccgatttgag ttgatatttg
      241 gctaaaaagg gcttgagtag caggtactaa tgcctgggta gccatttgat cagatgaatc
      301 aacgtacact actacactct tgggatttcc actgtttaaa tcttcataat ccggaggaag
      361 tatgatagca gccttcacca cgccggtttc aaccatttgc ctggctctat ctggatctga
      421 tatgatgtct ttcacttcat agaggtccat tcctttgatg tgggtgaggg ttgaatcagt
      481 taccatcccc tgggactgtt taaccacgac aactggtatg ttttcgattg atccccccat
      541 accgtagcca aaaagggcta tcataagtat ggggaatatg aatatggaaa ttaaacgcgg
      601 tttatgtctc cataacacta gtaaatcctt cttaatcatc cagtagattt tttttgtttc
      661 catcatatta ttcgcctgcc tcttcttttt taaccttttt tgcagtcact ttcgtgaaaa
      721 catcttctaa agaaggatct ttggttgaaa ttgaggtaat atttcctcca gtttccatta
      781 cgactgatat aacttgggtc acagcatttt cagtgttggc cagtttaaat gttaacctcc
      841 cggatggatg agtttcgatt tccaggacag acgggatttg ataaagtctt ttaattagtt
      901 cactatcaga attacttatc ataaggctca tttcccgagc attatcaatt tctgatttat
      961 ttacaattgt ttttaatcta ctaaaggagg gatggctaat aatttctgat tcttccaact
     1021 ctcttacgat ttctccgaca ttactagctt tattatcatt tttagtaagg atgctatctt
     1081 tcagcccctg tggggtgtca aaggccgcaa gctttcccaa gttaataatc ccaatgtggt
     1141 cgcagagcat atccacctcg tacatatcgt gggaacaaag gatgatggtg tggcccttct
     1201 gattcaattc atcgattagt tcccagagca cgcttttggt ggtaggatcc aaaccaatgg
     1261 tgggttcatc caaaaatagt atgtcgggtt ggtgaaccag actggccacc accgatactt
     1321 tttgcctttg tccaccagac atctgccgga tcagcttatt ttctgcgtac tttatatcca
     1381 ccagttccat taactcgttg atccgctgtt ctttaaggtc gcggggcatc ccataataat
     1441 cagcacagag ttctgcattt tcccgggctg taagatcacc ataaagactt accagttgtg
     1501 gtaccatccc aattttctga cgcacctcat ctggtgattt gataatgtcg aatcctgata
     1561 cttgtgcagt gccagaggtg ggaggtatta aacaggttaa catttttatt gtagtggttt
     1621 taccggctcc gttaggacct aaaaacccaa aaatgctctt gtttttcact tttaaatcca
     1681 aagcatccac agctacgaag tttccgtagc gtttggtcag atcaaaagtt tctatagcgt
     1741 atttcataag ggtaatctcc tatcttgttt tgtatccaaa caaatcatcc atgaattcat
     1801 cccaaatatt gatgttatca tgttcccggt tacattgggc aatttttcgg atattttcta
     1861 ggtttttttc tccatcagcc gtgattgcat aatatttaac tcttcttttt ccctgttttt
     1921 cccaggcacc acatatcatt ccttttttct ctaaatcatg tagaaccgga taaatcatgc
     1981 tggcacttgg cattttttta ttgtaaggac ttgatttgtg tatctgggtc ataatttcat
     2041 acccgtgctg tcttttcttg ctgattagcc ataggatcat tattttacca aaagccttca
     2101 ttaatcctcc aattatcttt ttatcagctt ctgctaagtt ttttatattt tctgcacacg
     2161 gatctttttt tttattcttt acagaatttt tattgctgtt atcttttgaa tttaaattgt
     2221 ttcccggcat ttcactccca gcatttatga taaattacga tatataatca ttcaatatat
     2281 cgaatttaga tatatcgatt atattagtac tgtgtttaga agtatataaa agatgcggtg
     2341 gatggaatga gatggtagca ttgaagcatc ttcaccataa aaaaaccttt ctgtaatggc
     2401 gttaaaatca atgatatcaa aaacagtata atactcagac ctttaatttg atttcacttc
     2461 atttatatag ttcaatcctt taatgattgt atagattttt attaaacaaa tccaattata
     2521 gaatgagcat gaatttaggg agttggtaac ttgagaattg gtttctccac tctggtactt
     2581 tttgcaaaac cattcgagga ctggctggaa agagccacct ccgatggctt taacatgata
     2641 gaaatactct gtgaaggtcc atactggcca aggatccttc tttcagagtg tgatggactt
     2701 gatatttttt cttcttacga tatagatctt tatctccatg ctcccaccat tgatcttaac
     2761 ccagccagtc ttaacccagg tattcgtgaa gaaaccatta aacaactcta tgaaactata
     2821 gacttagcat ctgaaattgg ggcaaaagct ataaccaccc atcccggttt aatacatcga
     2881 ctagaggata gaattaggaa tatggctttg tactattcaa ttgaaaccct taaagatgct
     2941 aatatctatg ctgaggactt tggaataatt ttttctgtgg agaacatgcc ccatcgttat
     3001 gcttatttct gcaacaatcc gcaggaacat acttatttca ccagagaatg tggttctcat
     3061 gctaccttgg atttgggtca tgccaacacc tctggagaat tagaaaaatt cttagatatg
     3121 ccccgaatac actactacca tgttagtgat aacaatggag aaaaggacca acatttatct
     3181 ttgggggaag ggaccctgga tttaggtgtg ttgaatggg
//
LOCUS       DAOQVW010000143         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_34657_length_3213_cov_3.038315, whole genome shotgun sequence.
ACCESSION   DAOQVW010000143 DAOQVW010000000
VERSION     DAOQVW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34657_length_3213_cov_3.038315"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PKK85_04045"
     CDS             complement(<1..124)
                     /locus_tag="PKK85_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNS25221.1"
                     /translation="MSSGKLIGVGVGPGNPDLLTLKAANILKEVPVICSPRSAES"
     gene            262..2109
                     /locus_tag="PKK85_04050"
     CDS             262..2109
                     /locus_tag="PKK85_04050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNS25222.1"
                     /translation="MDNGFFCSKCGMILTNCKCTHLKNRPQPSPVKGAVDLQPGSSRE
                     KPNLSRYKHSSSVNKPLARKETRISPSRVKELKGMYPHVDEEILEEFPFPSPREGQLN
                     IIRCLSEAIEQGYPYLILEAGTGTGKSAVATTLARMYQPAYILTMTKQLQAQYASEFG
                     YPLVKGRGNFACLDSNLDLNCDMGTCQTTPNSQEFSCDYGVTKSPFEGGYAFQDAFGA
                     PIYFRSQDHCPYWEQKAESAESSITLMNYDYALLELNYVKHFGKRQLMVLDEAHNLED
                     KLMRRLEVNLSRQRLEKDVKKTIPRSMMDYRDPEEWILFLEALYDSYAELSVKEMPKN
                     QADRVNSTKLRLSELLNNLEDQPENWVVDTDTAGVSFKPLRVDGYTKNRLFQYADACL
                     FMSATILDKELFSKWLGLEPDEVYYLEVPSPFPAGNRPVHMNLAGKMSQRLIKRTAPK
                     TIPILEKIIKHHKYEKGLIHTHNYKCQQYVMNQLNNSRLIGHNSQNREYALYQFEKTR
                     EPRILVSPSMSEGVDLPYEQCQFQVIYKIPFPYLGDPQVNERRKQDPRWYAYKTVITL
                     LQAYGRGMRAEDDYCETYILDGNIRMILTGKMYRHLIPRYFKEAIVRNE"
     gene            complement(2244..2319)
                     /locus_tag="PKK85_04055"
     tRNA            complement(2244..2319)
                     /locus_tag="PKK85_04055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2281..2283),aa:Arg,seq:tcg)
     gene            complement(2714..3049)
                     /locus_tag="PKK85_04060"
     CDS             complement(2714..3049)
                     /locus_tag="PKK85_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNS25223.1"
                     /translation="MRCLPDLDEYGDLIADGLNLIAVGIIYNIPLYIVSAIFIISALG
                     KFDLSFASSSSQITIAMVAFFISIVFIPAGGNMAYEEKFKEAFDIKRIFDFYKKILDG
                     LDTFHICYL"
BASE COUNT         1027 a          721 c          671 g          794 t
ORIGIN      
        1 ttgattcagc cgaacgaggg gaacatatga ctggtacttc tttaagaata tttgctgcct
       61 ttaaagtcag cagatctggg tttcccggcc caacaccgac tcctataagt tttcctgatg
      121 acatgtgtta actcctatct taatgattat ttgaaatcgt ttataacctt gatagtgagg
      181 gataatatga ataaagccgt tatttaaggt ttgatctggg gcttccacct atttatatca
      241 ccagcacaat ataggtaatc catggataat ggctttttct gcagtaagtg tggtatgata
      301 ctgactaact gcaaatgtac ccacctgaaa aacaggccac aaccatctcc agttaagggg
      361 gctgttgatt tacaaccagg atcatcccgg gaaaaaccta acttaagtag gtataagcat
      421 agttcatcgg tgaacaagcc cctagcaaga aaggaaaccc gcatatctcc ttcacgtgtt
      481 aaggagctga aaggtatgta tccccatgtt gatgaggaga tcctggaaga atttccattc
      541 ccctcccccc gggaaggcca gctgaatatt attaggtgct taagcgaggc cattgaacag
      601 ggttatccct acctgatctt ggaggcaggc actggcaccg gtaaatcagc ggtagccact
      661 accctggcac gcatgtatca gcctgcctac atcctcacca tgaccaaaca gttacaggcc
      721 cagtacgcct ctgagtttgg atatcccctg gttaagggtc ggggaaattt tgcctgcctg
      781 gacagtaatc tggatctgaa ctgtgatatg ggaacttgtc aaaccacccc caactcccag
      841 gagttcagct gcgattatgg ggtgactaaa tcaccatttg agggaggata tgccttccag
      901 gacgcctttg gggcacccat atacttccgg tcacaggatc actgtcccta ctgggaacag
      961 aaggccgaat cagctgagag cagcatcacc ctcatgaact acgactacgc cctcttagag
     1021 cttaactacg tgaagcactt cggtaaaagg cagttgatgg tcctggacga ggcccataac
     1081 ctggaagata agctgatgcg gcgtttagaa gttaacctct ccaggcagcg actggaaaaa
     1141 gatgtgaaaa aaaccatccc ccgatccatg atggattatc gggacccaga ggaatggata
     1201 ctctttttag aggcattgta cgattcctat gcagagttaa gcgtgaagga gatgcctaaa
     1261 aaccaggccg accgggtgaa cagcactaaa ttacggcttt cagagctttt aaacaacctg
     1321 gaggaccagc ccgagaactg ggtggtggat actgatacgg cgggggtgtc cttcaagcca
     1381 ttgagggtgg atggttacac caaaaaccgg ctcttccagt atgctgatgc ctgtctgttc
     1441 atgagtgcca ctatactgga taaagaactt ttcagcaaat ggctgggcct ggaaccagac
     1501 gaggtatact acctggaggt tcccagccca ttccctgctg gcaaccggcc ggtgcatatg
     1561 aatctggctg ggaagatgtc ccagcgtctc atcaagcgca ccgcccctaa aaccattccc
     1621 atactggaga agatcataaa gcaccataag tacgagaagg gactgattca cacccacaac
     1681 tacaaatgcc aacagtacgt catgaaccag ttgaacaact cacgactcat tgggcataac
     1741 agccagaacc gggagtatgc cctttaccag tttgagaaaa cacgtgaacc ccggattctg
     1801 gtgagtccat ccatgagtga aggggtggat ctgccctatg agcagtgtca gttccaggta
     1861 atatataaga tacccttccc ctacctgggc gacccccagg taaacgagcg ccgcaaacag
     1921 gaccctcgat ggtatgccta taaaacagtt ataactttgc tccaggccta tggacggggc
     1981 atgcgggccg aggatgatta ctgtgaaacc tacatcttag atggcaatat acgtatgatt
     2041 ttaaccggga agatgtaccg ccacctcatt ccccgctact tcaaagaggc cattgtgagg
     2101 aatgagtaaa taatcaaaat cagaaaacta tagccgataa atattcattc taatcctgat
     2161 caagaaccga tgcagcccat agccaccgat tttgaaattt aagaatacaa tgaaaatcag
     2221 aattgaagat taataaaaat tagcggacct ggagggattt gaaccctcga ccttgggatc
     2281 cgaagtccca cgtcatattc caggctagac tacaggccca taaagtgcct aacaaatcat
     2341 aggcaaatca cttgtaggtg tttttcactt ataaaactat ccgtttccta ttataaatag
     2401 catttaactc catttaggta tggaaaaaaa aataaaaaaa atgaaaatta attataaagg
     2461 attattattt agctaagtaa ttataattag ataaaggata tgttaatcca ataaattgtt
     2521 ccaaaaatta ttgttgttat tcttgcacgc cttcactata tatgaatccc cggaagcggc
     2581 ccccaacaag aattaaatag gaatttaaca ataataagag caataatata caagtccata
     2641 aaatgttcct atataaattt ttaagtaggg actggttaaa aggaaagcca aactcaaaat
     2701 ttccacaata aaactataaa taacaaatat gaaaggtatc tagtccatcc aatatttttt
     2761 tataaaaatc aaagattctt ttgatatcaa aagcctcctt gaatttctct tcataagcca
     2821 tgttaccccc agcagggatg aatactatac tgataaaaaa tgctaccatg gcaatggtaa
     2881 tctgggatga ggagctggca aagcttaaat caaacttccc caaggccgat atgatgaaaa
     2941 tggcagaaac aatatacaag ggaatgttgt aaatgatgcc tactgcaatc aaattcagac
     3001 catcagcaat caaatcacca tattcatcta aatcaggcag gcatctcatc aaacccaagg
     3061 attgtgcttt ttataactcg caaggaatag cccccaacca ttatcccagg aatttattag
     3121 aaaagacccc agtgatagga gaatgacttt taaaaactta ttagaatccg tgaaaggata
     3181 gtgaaacaca tctacaaaaa catgaccaat acc
//
LOCUS       DAOQVW010000144         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_35140_length_3184_cov_3.172260, whole genome shotgun sequence.
ACCESSION   DAOQVW010000144 DAOQVW010000000
VERSION     DAOQVW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35140_length_3184_cov_3.172260"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKK85_04065"
     CDS             <1..297
                     /locus_tag="PKK85_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25224.1"
                     /translation="EVGGEPSFAMCTRCGACTMACPNEEVTLVEMDKVIDGQIVKRNR
                     IQYSPEKCTQCGDCVEVCPYNMLKLTDEKVPLKGFCILCDQCIPACPHEALSLK"
     gene            complement(1019..1213)
                     /locus_tag="PKK85_04070"
     CDS             complement(1019..1213)
                     /locus_tag="PKK85_04070"
                     /inference="COORDINATES: protein motif:HMM:NF021636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HNS25225.1"
                     /translation="MCESSVYSTIGKKIMEDVISIKIDGEDIKLLDILNQPLSIKGRI
                     VEIDLDKHRIYVEVSGESTV"
     gene            complement(1496..2797)
                     /locus_tag="PKK85_04075"
     CDS             complement(1496..2797)
                     /locus_tag="PKK85_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HNS25226.1"
                     /translation="MLDLCLINCKLELGIGKVCLGIKKGKIVSIKRSPASAHETLDLR
                     GKLVLPGLIDSHVHFRDPGLTYKEDFRSGSTAAAAGGFTTVLDMPNNIPPTSTPQAFL
                     KKRKIAEKKSLVDFGLHAGVVDPHDIGLLAELRPVSFKIFMDLVDTTFLQEASVNLSG
                     CDLELPLSLHPEDPTTVHHYTRTMKKEGDQTSLYAQARPPEAEDIAVAQAIAISKKMK
                     QKIHLCHLSTIKSYQMTKNAQNNGLPVSFEVTPHHLFLDASYLERWGNLAKTNPPLRD
                     HEHKLGITELAGIDIVATDHAPHTLEEKNQDVWNAPPGVPGLETALPLLLTLVNQGKL
                     SLNHLKRILCENPARIFNIPHKGFLKEGMDADLVVVDLAQEGVINSDKFHSKAKYSPF
                     EGFKVQGMPVMTLVRGNVVMEEGNVLENKGKFIYSQINREK"
     gene            2929..>3184
                     /locus_tag="PKK85_04080"
     CDS             2929..>3184
                     /locus_tag="PKK85_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNS25227.1"
                     /translation="MDCTVNLVKDILARDRKTFLKDVDKRPEPKEVESDIPLLADFTE
                     YNPLHKGHLHCLLEAKKAVPEGIFVAVVPGLFERSGRGLPY"
BASE COUNT          915 a          703 c          661 g          905 t
ORIGIN      
        1 gaagttggcg gcgagccttc ctttgccatg tgcacccggt gcggggcctg taccatggcc
       61 tgtcccaacg aggaagtaac cctggttgaa atggacaaag tcattgacgg gcaaatagtt
      121 aaacgcaacc gtatccagta cagcccagaa aagtgtaccc agtgcggcga ctgtgtggaa
      181 gtatgtccct acaatatgct gaaactcacc gacgagaaag tcccactaaa gggattctgt
      241 atactctgtg accagtgcat accagcctgt ccacacgaag ccctttctct caagtaaaaa
      301 acaagaccaa tctttaggtg gtgcaaccca cctaaaacca cctttttttc aatccccatc
      361 caattaagtg actttttcta gacctacaag tcctttttct atctcaggtg cgtatagcca
      421 ggttaccacc agcaataaag ggccggtttt tatggaaaaa aaatttctgg atattaaggt
      481 agaacattcc aagtgccagg ctactctgcc cctgttacga atccccttac cacgctacac
      541 agaaaagtta aaaataagat aatcccgggg atagaaggca acaccacaga gcatgctgat
      601 ggctacgctg gaagcaccga aaccacatgt aaggcgaaca ggatcagatc gatatacacg
      661 tggaaaatca aataggtcta tcccgatatc ttgccacgac tagtattttg gggcagcgat
      721 taatgaatga acagcttaga aggtaaccga tggcctgtac aaaaaaagga accccggttc
      781 atccactgtc atgactggca aagtagattt gaaaccaggc ataagatgtt caccaaagcc
      841 ataggaatag ttatagtatg agaaacagca ttccatgaca attaccgggg aggaagagag
      901 ggatgcagtt acttcacctt aatttaaaag gagactaata ggctgtaatt ttaaaaaaaa
      961 tctcaatacc cattattttt tctaagacct catcactgga actcaaagat ttcttagatc
     1021 aaactgtgga ttcaccagag acttccacat aaattctgtg cttgtccaga tcaatctcca
     1081 ctatcctgcc tttaatggaa aggggctggt ttaatatatc cagtaattta atatcttcac
     1141 catcaatttt gatggatata acgtcttcca ttattttttt tccaatagta ctgtaaacgc
     1201 ttgattcaca cataacaata gcatctgtct tggaatatta tattaattta tagatttttt
     1261 aaatattttt tcgcagcatt tacgtccatt aattttccca aaaattcttt ataacagcct
     1321 ccaagttaaa tcgtcaagtt taaatcttat gtatttttgg agaaagatgc aaatatgggg
     1381 ggtgtgagat aatgaatggt aaaacaggga ttctattact catataaact gtgatggtcc
     1441 tcttgatgac tggaggaaaa aaaattcaaa aacgggatta tttttttccc aaaacctatt
     1501 tttcacgatt tatctgagaa taaatgaact ttcctttatt ttctagaacg ttcccttcct
     1561 ccataactac attacccctt accagggtca tcactggcat cccctgcacc ttaaaacctt
     1621 caaatgggga atatttggct ttggagtgga atttatccga gttgattaca ccttcctggg
     1681 ccaggtccac caccactaga tcagcatcca taccctcttt aaggaagcct ttatggggaa
     1741 tattaaatat cctggctggg ttttcacata aaatcctctt taaatgattt aatgaaagtt
     1801 ttccctggtt tacaagggtt aaaagtagtg gaagtgccgt ttccaggcca ggtacacctg
     1861 gtggtgcatt ccacacatcc tgatttttct cctctagggt gtgaggggcg tggtcagtgg
     1921 caacgatatc tattcccgcc aactcagtga ttcccagttt atgttcatga tccctgaggg
     1981 gagggttggt cttggccagg tttccccagc gctccagata agaggcatcc aggaacaggt
     2041 ggtgaggagt tacttcaaaa ctcactggca atccattatt ttgagcattc tttgtcatct
     2101 ggtatgattt tatggtgctc aggtggcaga gatgtatctt ctgcttcatc ttcttggaaa
     2161 tggcaatagc ttgggccacg gcaatatctt ctgcctctgg gggccgtgcc tgggcgtaga
     2221 ggctggtttg gtcaccttcc tttttcatgg ttctggtgta gtggtgcacc gtggtaggat
     2281 cctcggggtg cagggacaga ggcagttcca gatcacaccc acttaagttc acagaagcct
     2341 cctgtaaaaa ggtggtatcc accagatcca tgaatatttt gaaggatact ggccttaatt
     2401 ctgcaagtag ccctatatca tggggatcca caaccccggc atgtagtcca aagtccacca
     2461 gactcttttt ttcagcaatc ttgcgttttt tcaaaaaagc ctggggggtg ctggtaggtg
     2521 gtatgttgtt gggcatgtcc agtacagtgg tgaatccacc ggcagctgct gcagtcgaac
     2581 cacttctaaa atcctcctta taggtcaaac ctgggtctcg gaagtgcaca tgggaatcaa
     2641 taaggcctgg aagaactaat ttacccctca aatccagggt ttcatgggct gacgctggtg
     2701 accttttaat ggagactatt ttaccctttt ttattcccaa acagaccttt ccaatgccaa
     2761 gttcgagttt acagttgatt agacacagat ccagcactat aaatcaccat aattttagaa
     2821 ataaggtctt aagattccta ttttttgtat atttccaatt tctctgttta ctatatacca
     2881 cttatataga aataatcata taaccttgat gaaatccaat acataaacat ggactgcaca
     2941 gtgaaccttg taaaggatat tctggcccgg gaccggaaaa ctttcctgaa agatgtggat
     3001 aagcgaccgg aaccaaaaga agttgaatca gatattcccc ttcttgcgga cttcaccgag
     3061 tacaaccccc tccacaaggg ccatttgcac tgccttctgg aagcaaaaaa ggcagttcca
     3121 gagggaatat tcgtggcagt tgttccaggc ctttttgaac gtagtggaag gggattaccc
     3181 taca
//
LOCUS       DAOQVW010000145         3169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_35389_length_3169_cov_3.856134, whole genome shotgun sequence.
ACCESSION   DAOQVW010000145 DAOQVW010000000
VERSION     DAOQVW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35389_length_3169_cov_3.856134"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..843
                     /locus_tag="PKK85_04085"
     CDS             151..843
                     /locus_tag="PKK85_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNS25228.1"
                     /translation="MFIVSPLIAGYVEAYIARRKYGRSTGAISALLIFILINAYSWLF
                     PKDPIVLNLFTMGGLALMIQAAFPILINYILFVVFIGTITWVIGYLGNILSNLTDKVV
                     RRSPEEKVEETGIEQTNRPDFLDTLEYPILTIPHISKGKIVKHLGMVVGEAVVGKKSP
                     KGISKIVSQDKLDDMELKKARERAIQELLEEARKIGGNTVVEVLIDYNTIGGISGSAT
                     IVTATGTAVVYE"
     gene            complement(870..1700)
                     /locus_tag="PKK85_04090"
     CDS             complement(870..1700)
                     /locus_tag="PKK85_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNS25229.1"
                     /translation="MQRHYQVILEGILAFLILIELLSLSLMTVGFIVGLKPETIYTLG
                     SLDVIIAFLILLDLTFFRIRYREVNTTIMKFIQENWAYIIAIFPLIFVSFNLLELFDL
                     KVIIGLMGIVRIYALFKVLFISARNVRKYPSKTKLDYATLALFLVIILGSYLFFAIET
                     GVNPTVPNYESAIWFTIVSMSTTGYGDVVPITLAGRIIGVILILTGMGYVSLVTATLA
                     YSFIDIFRAKSQEASEKFKATTQDLYRRAVKNEEKLEEILDKIDKINQRLDEMEKKKN
                     "
     gene            complement(1800..2378)
                     /locus_tag="PKK85_04095"
     CDS             complement(1800..2378)
                     /locus_tag="PKK85_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNS25230.1"
                     /translation="MELSRSTTFPTKKIKPISTKISNECVDKIGGKDMQRTGTALVLI
                     ILGILVLAFPLLGLIPLSLLTGFFVLILGIGLLIGGIGQIGESVGLGILEIILGILAL
                     IMGFGFIFNPGLFSWLIGLIVWIVGLFLVIAGVIGILSKVGGSRWNGIVAIIIGILYI
                     IVGTWIKDPIILGALIGLWLLLTGVVMLLMKE"
     gene            complement(2405..>3169)
                     /locus_tag="PKK85_04100"
     CDS             complement(2405..>3169)
                     /locus_tag="PKK85_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNS25231.1"
                     /translation="TLEIESGLNDGIAVPFLLLFLGFGLAEETFRPINYFLVTTFEQI
                     VFGVLIGLGVGLIGGWMVLQAKKKSWITKTYQRLALLALAILSWLITDQLGGSGFIAA
                     FVGGLSTGFIFEDAGDILTDFTRAEGMLLSLSVFFIMGALITPLLSFVTWKIIFYAVL
                     SLTLIRMLPVAISLIGTKISLDTVLFLGWFGPRGLASIVLALVALEAIPEFPGKDTFT
                     LTILVTVFLSIILHGITALPLSELYIKRDKKVKGDS"
BASE COUNT         1030 a          636 c          565 g          938 t
ORIGIN      
        1 ctcgaggttg ggaacttgct tgcatttatg gagaactttc tgatagaaaa aaagtgggcc
       61 tttggggcta tttttatagg ggtcttactt ggttttcttt ctgcagttat ctgcgttaaa
      121 gggaatttgg ctatttttgg atttaacatc atgttcatag tgtcccctct tattgccggc
      181 tatgtggagg cttatatagc tcgccgaaag tatggtagaa gtacaggggc cataagcgcc
      241 ctgctaatat tcatacttat caatgcttac tcctggctct tccccaaaga tcccattgta
      301 ctcaatctgt ttaccatggg aggattggcc ttgatgattc aggcggcatt ccccatactg
      361 ataaattaca tcctttttgt ggtgtttata ggcaccatta cctgggttat tggatatttg
      421 ggcaacatct tatccaatct aactgataag gtggttagaa gatctcctga ggaaaaggtt
      481 gaagaaacag ggattgaaca aactaaccgt cctgattttc ttgatacatt ggaatatcct
      541 attctaacca ttccacacat atcgaagggt aaaatagtca aacacttggg aatggtggtt
      601 ggtgaagcgg tggttggcaa aaaaagccct aagggaattt ctaaaatcgt gtcccaggat
      661 aagctagatg atatggagtt aaaaaaggcc cgtgaaaggg caatacagga gctcctagag
      721 gaagcaagaa aaattggggg aaatactgta gttgaagtat taattgatta caacactata
      781 ggagggattt caggaagtgc taccattgta acggctactg gaaccgcagt tgtttatgaa
      841 tagaatggat atgctctata aattaaaaat tagtttttct ttttttccat ttcatccagc
      901 ctctggttga tcttgtcaat tttgtccaaa atttcctcca gtttttcttc gtttttcacg
      961 gcccttctat acagatcctg agttgttgcc ttaaactttt ctgatgcttc ctgacttttg
     1021 gcacggaaaa tatcaatgaa agagtaagcc aaggtggcag ttaccagact aacatagccc
     1081 ataccagtta aaattagaat tactcctatt atccggccgg ctaaagttat gggaaccaca
     1141 tctccatatc cggtggtgga catggatact atggtaaacc agattgctga ttcgtagttg
     1201 ggaactgttg ggttaacacc agtttcaatg gcaaagaaga ggtaagatcc tagtatgatc
     1261 actaaaaaaa gtgctaatgt agcataatct aatttagtct tggaaggata tttacgaaca
     1321 ttccgagcag atatgaaaag tactttaaag agggcataaa tcctcacaat ccccattaac
     1381 ccaataatga cttttaagtc aaataattcc aaaagattga atgatacaaa gattagtgga
     1441 aaaatggcta ttatgtaagc ccagttctct tgaataaatt tcattatagt agtgttcact
     1501 tctctatatc ttatccggaa aaatgttaga tccagaagta tgagaaatgc aattatcaca
     1561 tccagacttc ctaaggtgta gatggtttct ggtttcagcc ccacaataaa acccactgtc
     1621 attaaactta gtgataacag ttcaattaaa atgagaaagg ctagaattcc ttctaatata
     1681 acctgatagt gtcgttgcat gcattctcct aatttaatcc caatcctatt tgagagctta
     1741 ttcttcttaa gttattacat ttaaaaataa aaaaaaatta gggcttgttg aaaatcagtt
     1801 cattctttca ttaacaacat tactactccg gtgagtaaca gccataaacc aatcaatgca
     1861 cccagaataa tgggatcttt tatccaagtc cctactatta tgtacaatat accgattata
     1921 atggccacaa ttccattcca ccggcttccc cccacctttg atagtatccc tataacacct
     1981 gcaattacca ggaataaacc cacaatccag actattaaac caattaacca gctgaacagt
     2041 cctggattga atatgaaccc gaatcccatg atcagggcaa gaattcccag aataatctcc
     2101 aaaattccta aacctacact ttcgcctatt tgacctattc cacctattaa aagaccaata
     2161 cccaaaatta acacaaaaaa tccagttagt aggcttagtg gtattaatcc taataagggg
     2221 aatgccagga caagtatgcc caaaataatt aaaaccaatg cagtaccagt tctttgcata
     2281 tctttacctc ctattttatc aacacactcg ttactaattt tggtacttat tggctttatt
     2341 ttttttgttg gaaatgtagt actccttgat aattccaatt tacatacagc ttaaggataa
     2401 tttattatga atcccctttt accttcttgt cacgttttat ataaagttct gataagggta
     2461 aagcagttat tccatggagt attatgctta aaaaaacggt taccaggatg gttaaggtga
     2521 aagtatcctt acccggaaat tctggaatag cctccaaggc cacaagggcc agtactattg
     2581 atgccagtcc tctaggtcca aaccagccaa ggaataatac ggtgtctaaa cttatctttg
     2641 ttccaataag agagatggcc acgggcagca tccgaatcaa agtaagactt aaaacagcat
     2701 aaaaaatgat cttccaagtt acaaaagata acaaaggagt aatcaaagca cccataataa
     2761 agaacacaga taaacttaga agcattcctt ctgcacgggt aaaatcggtt agaatatcac
     2821 ctgcatcttc aaaaataaat ccggtagata gtccccctac aaaggcagct ataaaaccgc
     2881 ttccccccag ttggtctgtt atcaaccagc taagtatggc taatgcaagt aaagcaagcc
     2941 tctgataggt tttagtaatc caactttttt tcttggcctg aagaaccatc caacccccaa
     3001 ttaatcctac acccaagcca attagcactc caaatacaat ttgctcaaaa gtagtgacta
     3061 gaaagtagtt aattggtcta aatgtttctt cggccaaacc aaaacctaag aaaagaagaa
     3121 gaaatggcac agctattcca tcatttaatc cactctcaat ttcaagagt
//
LOCUS       DAOQVW010000146         3132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_35991_length_3132_cov_3.199220, whole genome shotgun sequence.
ACCESSION   DAOQVW010000146 DAOQVW010000000
VERSION     DAOQVW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35991_length_3132_cov_3.199220"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PKK85_04105"
     CDS             <1..941
                     /locus_tag="PKK85_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS25232.1"
                     /translation="KPAVTDIIGWDMDDRTLLMLTASVEQNSQHPLAEAMVTKAQDND
                     ITLFDANNFDTYPGKGVSAMVRGKEVLIGNRQLLNAYEVEISEENENKISTVEAAGKT
                     AILVAVNHKFAGIMGVADTLKESAAPAIIELKKMGIQVGMITGDNQQTADSIARQVGI
                     EKVMAEVLPPDKASEVKRLQDGGEVVAFVGDGINDAPALAQANVGIAIGSGTDVAIES
                     GEIVLIKDNLLDAVAGLQLSRKVMGRIKLNLFWAFAYNVILIPVAAGVLYPTLGITFR
                     PEYAGLAMALSSVTIVTLSLLLKGYVPPAKKLVVQK"
     gene            948..1127
                     /locus_tag="PKK85_04110"
     CDS             948..1127
                     /locus_tag="PKK85_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNS25233.1"
                     /translation="MNDPIVKEVICMAVDPICKMDVDEKNAKWVSEYKGKKYYFCAPG
                     CKKAFDEDPAKYAEE"
     gene            complement(1398..2648)
                     /gene="truD"
                     /locus_tag="PKK85_04115"
     CDS             complement(1398..2648)
                     /gene="truD"
                     /locus_tag="PKK85_04115"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNS25234.1"
                     /translation="MLNADTYITTQRGIGGRIRTRYEDFYVEEILENQPSGEGPNTWI
                     FIDKIGRNTLDVVLDIARELKISRKRMGFAGMKDKAAHTRQWLCISNTAPEQLFGLEK
                     KLYGVKILKIKANQKKLRIGQLVGNQFRILIRETKSPAQDVVTASEVLDDLKKRGVPN
                     YYGWQRFGQKRPNTHLVGEALVHNDFKGAVDSYIGNPYPDEAPHIQATRRMYDAGDWE
                     EALDNMPSGMRYEKMMLRTLLREKRKKEELNEKSYIKALYSLPKPLNRMFVHAYQSFL
                     FNKVVSERSQLGVDKYVEGDIIIDNQEHLVHKIGEDLDQQIKNFQIHPTAPLYGTKVP
                     LATGEVGKVEEQVLKEYKLKKEDFRVPMMPRLGSHGLRRAVRFKIWDVSSEPTPDGVL
                     VEFSIPKGCYATAVLREIMKVDVY"
     gene            2758..>3132
                     /locus_tag="PKK85_04120"
     CDS             2758..>3132
                     /locus_tag="PKK85_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNS25235.1"
                     /translation="MKIIKIMPCLDMKDGRVVKGVHFVDLKDAGDPVECAALYQEEGA
                     DELAMLDIAATIENRGARLEWVENVASVINIPLTVGGGISSLEDIELTLNAGADKVSM
                     NSSAVHNPELIRKAAQKFGSERI"
BASE COUNT          782 a          700 c          786 g          864 t
ORIGIN      
        1 gtaaaccagc agtgaccgat attattggct gggatatgga tgaccggacc cttttaatgc
       61 tgacggccag tgtagagcag aattcacagc atcccctggc tgaggccatg gtaaccaaag
      121 cccaggacaa cgatataacg ttatttgatg caaataactt tgatacctac cctggtaagg
      181 gtgtttccgc aatggttagg gggaaagaag ttttgattgg taatcgccaa ctcttaaatg
      241 cctatgaggt ggagatatct gaagagaatg agaataagat atctacggtg gaggcggcgg
      301 gtaaaactgc gatactggtg gcggttaatc ataaatttgc cgggataatg ggagtggccg
      361 acaccctgaa agaaagtgct gcacctgcca tcatcgagtt aaagaagatg ggaattcagg
      421 tgggcatgat caccggggac aaccagcaga ccgccgacag tatcgcccgg caggtgggaa
      481 ttgaaaaagt tatggccgag gtactgcctc cagataaagc ctccgaggtt aagagactgc
      541 aagatggggg tgaagtcgtg gctttcgtag gggatgggat aaacgatgcc ccagccctgg
      601 cccaagccaa tgttggcatc gccatcggca gtggaactga tgtggccatt gaaagcggtg
      661 agatcgtgct gatcaaggat aacctgttgg atgccgtggc cgggttgcag ctcagccgca
      721 aggtaatggg acgcattaaa ctcaacctct tctgggcctt cgcctacaac gtgatcctca
      781 tccccgtggc ggcgggagtt ctgtatccca ccctaggcat cactttccgg ccggaatatg
      841 caggattggc catggccctt agttcagtga ccatcgtgac cctatccctg cttttgaagg
      901 gttatgttcc cccggctaag aaattggttg tgcagaaata attaaatgtg aatgatccca
      961 tagttaaaga ggtgatatgt atggccgtgg accccatatg taaaatggat gtggacgaga
     1021 aaaacgccaa gtgggtaagt gaatacaagg gcaagaagta ctatttctgc gctccgggct
     1081 gtaaaaaagc ctttgatgaa gacccagcaa agtacgctga ggagtaggat ccggatccta
     1141 agaacatgga tagtatcctt aaccggagaa tatttgtatt aatccatgat gttgggctta
     1201 ttattagcga tagtcattgc cattattgct aaattcaatt actggttagg aatgttaacc
     1261 cttgttgatt aaggagcgtg tttgtacacg agaacatgaa aacaagttga atctattttt
     1321 ttctttatta agctggcgcg tgccaagatt taagttaaga tcctatgttt aactcccata
     1381 acaattcctg tagaaaatca gtagacatcc accttcataa tctcccttaa aaccgctgtg
     1441 gcgtagcaac ccttgggaat ggaaaattca accagcacac catctggagt gggttccgag
     1501 gacacatccc agattttaaa gcgtactgcc cttcgcagac catgactacc taatcggggc
     1561 atcattggta ccctaaagtc ttcttttttt aacttatatt cctttaaaac ttgttcttct
     1621 actttaccta cctcacccgt agcaagtggt acttttgtac cgtagagggg tgcggtgggg
     1681 tgtatctgga agttttttat ttgttggtca agatcctccc cgattttgtg caccagatgt
     1741 tcctggttgt cgatgatgat gtccccctcc acgtacttgt ccacccccag ctgggatcgt
     1801 tcactgacaa ctttattaaa taggaacgat tggtaggcat ggacgaacat ccgattaagg
     1861 ggcttgggaa ggctgtacag ggctttgatg taggattttt cattcaactc ctcttttttc
     1921 cttttttccc tcagtagggt gcgaagcatc atcttctcgt aacgcatccc actgggcatg
     1981 ttatctaggg cctcctccca atcccctgca tcatacattc tacgggtggc ttggatgtgg
     2041 ggggcctcat cagggtaggg gtttccaata taagaatcaa cagctccttt gaaatcattg
     2101 tgtactaggg cctcccccac caggtgggtg ttgggacgtt tctgaccgaa cctctgccag
     2161 ccatagtaat tgggcactcc cctttttttt aaatcatcca gcacctccga ggcagtaacc
     2221 acgtcctgtg ctgggctttt agtttcccgg atcagtatac ggaattggtt tcccaccagc
     2281 tgccctatcc ttaatttctt ctggttggcc tttatcttta ggatttttac tccgtaaagc
     2341 tttttttcaa gaccaaacag ttgctccgga gcagtgttgc tgatgcaaag ccactgacgg
     2401 gtgtgggcag ctttatcctt catcccagca aagcccatgc gctttctact tattttgagc
     2461 tcccgggcta tgtctagcac cacatccaaa gtgtttctac cgattttatc gatgaaaatc
     2521 caagtgttgg gcccttcccc actgggctgg ttttctagta tctcctccac gtagaaatcc
     2581 tcgtatctgg ttctgattcg gccgccaatt cccctttggg tggttatgta ggtgtctgcg
     2641 ttgagcatgg ttttctccag gaatagattt gctacactga ttatgtgtac tccatgtatt
     2701 aggatatggg tactcatatt aggttcaaga taatattagt ttacggagtt gctaaccatg
     2761 aaaatcataa aaatcatgcc gtgcctggat atgaaggatg gaagggtggt gaagggagtt
     2821 cattttgtgg atttgaagga cgccggtgac ccggttgaat gtgctgcttt gtaccaagag
     2881 gaaggagccg atgaactggc catgttagat attgccgcca ccatagagaa ccggggggcc
     2941 cgtcttgaat gggtggagaa tgtggcctca gttattaaca ttcccctcac tgtaggggga
     3001 ggtatcagta gcctggaaga tattgagctc accctcaatg ccggggcaga taaggtgtct
     3061 atgaacagtt ctgcagtgca taatccagaa ctgatccgga aagctgccca gaaatttggc
     3121 tccgaacgta tc
//
LOCUS       DAOQVW010000147         3112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_36304_length_3112_cov_3.027478, whole genome shotgun sequence.
ACCESSION   DAOQVW010000147 DAOQVW010000000
VERSION     DAOQVW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36304_length_3112_cov_3.027478"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /gene="tfb"
                     /locus_tag="PKK85_04125"
     CDS             <1..448
                     /gene="tfb"
                     /locus_tag="PKK85_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876518.1"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HNS25236.1"
                     /translation="SIEGVVAASLYAACRRCNVPRTLDEIAEVSRVSKKEVGRTYRFL
                     TRELNIKLPPTSPVDYVPRFASELNLSGEVQSKAIEIIEKAMEKGLTSGRGPTGVAAA
                     ALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKELTEQLDMGVTL"
     gene            complement(1007..3112)
                     /locus_tag="PKK85_04130"
     CDS             complement(1007..3112)
                     /locus_tag="PKK85_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNS25237.1"
                     /translation="MMEEYNQTYKPSRFYWIGRTITIWIGGFLGFIIIDALSIGLKFD
                     TWQTAFLAAGVVGLLNALFWPLLSRILLPFMVFTIGFGALLLNALLLWVAGDILMGIS
                     IEGPALFLTPIAMAACTALLSTLLTIDDDATYYRSVIRKDIQKGVRSKQKKPGVIFLE
                     IDGLAEPILKEALNKGHMPTLESWLDRGSHKLIHWETDLSSQTGASQAGILHGNNQDM
                     PAFRWVEKDKNNKIMVSTGFSDAQVIEERISNGKGLLSNRGASRSNLFSGDAEDVIFT
                     YSQLKNLKRYYSKAWYYVYSNPSNFARILTLFFWDVLLDYTSQILHWARNIQPRIRRG
                     FTYPFIRAGANVFLREVTTAILIGDILEGKIDVSYVTYLGYDEIAHHSGTRDWDSFYA
                     LRKLDKQFHRLEQAKIHASRPYYLVVQSDHGQTNGSTFFQRYGKTLEDLVQELMPPET
                     KIYSELFSNEDHFGQAIQGPLKDGKQYLKTKKDAMTYHGQFILNLAVKEIDESPVVKG
                     RVMEYLRRHEIDPTPSREEVKSEEAQVIVLASGNLGLIYLTDHERRLSFEEIKILYPD
                     LIPGLAQHEGVGFIMVCSDEYGPVVIGSEGVHYLYDGSIEGKDPLTVFGNNAVQHLIR
                     TNSFQNTPDILVNSAYYPITGEVAAFEELVGSHGGLGGEQSLPFILYPSHWKLDSEKI
                     VAAEHLHWLLKKELERLNI"
BASE COUNT          901 a          655 c          651 g          905 t
ORIGIN      
        1 tagcatagaa ggggtggtag ctgcatcatt atatgctgca tgccgtcgat gtaatgttcc
       61 ccgaaccttg gatgagattg cagaagtgtc cagggttagt aaaaaagaag tgggcagaac
      121 ttaccgtttc ctgacccggg aactgaacat aaaactaccg cccacctctc ctgtagacta
      181 cgtaccccgg ttcgcaagtg aacttaatct ttctggtgaa gttcagtcta aggccattga
      241 aatcattgaa aaagctatgg aaaaagggtt aacttcaggt agaggaccca ctggtgtggc
      301 tgcagccgcc ttgtacattg catcagtact tttgggtgag agaaagactc aacgtgacgt
      361 ggcagacatc gccggtgtaa cggaagtaac tatccgtaac cggtacaagg aactgacaga
      421 acagttagac atgggcgtga ctctctagtc actccccact tcattttatt aggtaatttt
      481 taaggtttaa atgcatccat tgaacttatc atcaatctat taaaaatacc gtaaaactgc
      541 ttaaaagatg tatctaagat ttcttctaat attaaaatta aattataaat tatagcatca
      601 gatcagtggg ttgttgaatt tgacagtttc aaaggtaaat tatttggatc cagatgatga
      661 acctccctgg atgaattaga actggtagta accaatgaaa tacgtcgaga aaatattaac
      721 atgtactgat gttaatggtt attttaaatc agaatatcca gactactaaa accctaaaac
      781 gagtttttta gtgacgaaga tcttctccat gtgaaattaa agtcagtgaa gaggttatga
      841 agttattgga tcatcccaga tttgggtcta caacttgtga agaattggac tactacatga
      901 caaacagcat tcaacccttc tacatctcaa gtaaggtttc catggcaaat catcaatgaa
      961 taactcaatt tataggctga taaattcttt aatttaataa tcctgattaa atattcaacc
     1021 tttccaactc ttttttgagt agccaatgga gatgttcagc cgccactatc ttttcagaat
     1081 ccagtttcca atgcgatgga tataagatga atggaagaga ttgttcccca cccaaacctc
     1141 catgactacc aactaattcc tcaaaagcag caacttcacc tgtaatgggg taataagcgc
     1201 tgtttaccaa gatgtctggt gtgttctgga aagaattggt acggatgaga tgctgcactg
     1261 cgttattgcc aaaaaccgtt aaaggatcct ttccctctat agatccatca tacagataat
     1321 gtactccttc agaaccaatg actactggtc cgtattcatc agagcagacc atgataaatc
     1381 ccacaccttc gtgctgtgct aggccaggta taagatcggg atagaggatt ttgatctctt
     1441 cgaagctcaa tcttctttca tggtcggtta aatatatcag gcccagattg ccagatgcca
     1501 gcacaattac ctgtgcttct tccgacttta cttcctcccg ggagggggtt ggatcaattt
     1561 catgacgtct taaatattcc attaccctac ctttaacaac cggagattca tcaatctctt
     1621 taacagccag attaagtata aattggccat gataagtcat tgcatccttt tttgtcttca
     1681 gatattgttt tccatccttt aaaggtcctt gtatggcttg gccgaagtga tcttcgttgg
     1741 aaaataattc actgtaaatt ttggtctcag ggggcattaa ctcctgcacc aagtcttcca
     1801 gtgtcttccc atagcgctga aaaaaggtgg atccattagt ctgaccgtga tcagattgaa
     1861 ctaccagata ataagggcgg gaagcgtgta ttttagcttg ttccaaacga tgaaactgtt
     1921 tatctagttt acggagagca taaaaagaat cccaatctct ggttcccgag tgatgggcta
     1981 tttcatcgta tcccaagtag gtcacgtaac tcacatcaat ttttccctct aaaatatctc
     2041 cgattaaaat tgcagtggtt acttctctca agaaaacgtt ggcccctgct cttatgaatg
     2101 ggtatgtgaa tccgcgcctg attctaggtt gaatgttcct agcccagtgc aggatctggg
     2161 aggtgtaatc cagcagcaca tcccagaaaa atagggtcag tatccgagcg aagttcgagg
     2221 ggttagaata cacgtaatac caggctttac tgtagtatct tttaaggttt tttagttggc
     2281 tgtaggtaaa tatcacatct tctgcatccc cggagaacaa attagagcga ctggcacccc
     2341 gattagaaag gagacctttt ccattggaaa ttcgctcttc gattacttga gcatcagaaa
     2401 atccggtaga aaccattatc ttgttatttt tatctttctc cacccagcgg aatgcaggca
     2461 tatcctgatt atttccgtgg agaatgcctg cttggctggc accagtctgg cttgagagat
     2521 cggtttccca gtggatgagt ttatggctgc ccctatctaa ccagctttcc agggtgggca
     2581 tgtgcccctt attcagggct tccttaagta taggttctgc cagtccatct atctccagga
     2641 atatgacacc cggttttttc tgttttgatc tcactccctt ttgaatatct tttctaataa
     2701 cactccggta gtaggtggca tcgtcgtcga tggttaaaag agtggaaaga agagctgtgc
     2761 aagctgccat agcaatgggt gttaagaata gggctgggcc ttcgatagaa attcccatca
     2821 gaatatcacc agctacccat aacagtaagg cgttgagtaa gagcgctcca aatcctatgg
     2881 tgaataccat aaatggcagt aaaatacggg ataatagggg ccagaaaagt gcgtttagaa
     2941 gacccaccac tccggcggcc aggaatgctg tttgccaagt atcgaactta agcccaatgg
     3001 aaagggcatc tatgattatg aagcctaaaa atccacctat ccagatggta atagtgcggc
     3061 ctatccaata aaaccgggat ggtttgtagg tctggttata ctcttccatc at
//
LOCUS       DAOQVW010000148         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_36781_length_3086_cov_3.220389, whole genome shotgun sequence.
ACCESSION   DAOQVW010000148 DAOQVW010000000
VERSION     DAOQVW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36781_length_3086_cov_3.220389"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKK85_04135"
     CDS             <1..558
                     /locus_tag="PKK85_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HNS25238.1"
                     /translation="TIMTGQYGLSMEQAQNVLKYTHPDNPVPVVFITSYDMIGKAGWW
                     SYFGGWNFQNSSGQNMVYSLAPANITTQNDTLVLQGENGVVAQITSSNITAGIVTSNN
                     QVALAHQVIVMVNGTTATYHLVSNQSTFSVMIIKEGDSYYTMAMNRELENSMFTRLFF
                     LQGAGLTRFSPIHAQPGVIVWNVAV"
     gene            684..1778
                     /locus_tag="PKK85_04140"
     CDS             684..1778
                     /locus_tag="PKK85_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS25239.1"
                     /translation="MTLEDRIRSIEEEIKKTPYNKATSHHIGKLKAKLSQLREESFKR
                     ASAGTKGKGFHLKKSGDSTVALVGFPSVGKSTILNQITNAESKIGAYEFTTLEVIPGV
                     MEYRGAHIQIFDIPGIISGAAHGRGRGREILSVARNADLVVMVLDVFQPQHQEMILQE
                     LDHIGIRPNQSPPDVRVKRRKVGGIKLASTVPLTHMDEKSIRSILNEYGVHSADVLIR
                     EDVTMDRFIDSLDSSIVYIPLLVVVNKIDLVDAHYLEEVKRKLPNALFIAADKQLNID
                     ELKEEIFQRLELIRIYLKPQGKKADMEEPLIVRKGSTVENVAQRLHRDFVRNFRHANV
                     WGKSVKFPGQKIGLEHVVEDEDIVRIIVKK"
     gene            1795..2571
                     /locus_tag="PKK85_04145"
     CDS             1795..2571
                     /locus_tag="PKK85_04145"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HNS25240.1"
                     /translation="MKLDDIIVSKAIVEGYMEDLLNYMEMDVAIGGGGPAGITAGYYL
                     AKAGLKVALFEKKLTIGGGMWGGGMMFNKIVVQEEGKRILEEMDIVSQEFQPGYHVAD
                     SIETVSTLLSRATKAGLKVFNLIEIEDVMIKQRGVEGLVINWSTVGMSGLHVDPITVR
                     SKAVVDATGHPCEVVKVVEDKVGPELHTKTGRIMGEKSMWADRAESIILENVGEVYPG
                     LYVAGMAANAVHGSPRMGPIFGGMLLSGERVAQILIEKLE"
     gene            2579..>3086
                     /locus_tag="PKK85_04150"
     CDS             2579..>3086
                     /locus_tag="PKK85_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HNS25241.1"
                     /translation="MILLLTGTPGTGKTTISRLLSQELEAKLVLVNQLVDQKHLYTGL
                     DPEKNYKIVDLPNLLGEMDEISQNYQGSKDWLIFEGHLSHYYQPADLVVVLRASPSIL
                     EKRLEKRGWKPEKIKENLEAEALDICAWEAGDIHGALAQEVDTTHSRPGDVVNTIMGI
                     IRGDLTLPL"
BASE COUNT          895 a          672 c          794 g          725 t
ORIGIN      
        1 accatcatga caggccagta tggactgagc atggaacaag cccaaaacgt acttaaatat
       61 actcatcctg ataacccggt tccggtggta ttcataacca gctatgacat gattggaaag
      121 gctggctggt ggtcctactt tggtggctgg aacttccaga attcttctgg tcagaacatg
      181 gtgtactccc tggctccagc caatatcact acccagaatg ataccctggt cttgcagggt
      241 gaaaatgggg tggtggccca gataaccagt tccaacatca ccgccgggat agttaccagt
      301 aacaaccagg tggccctagc ccaccaggtg attgtgatgg ttaatggaac caccgccacc
      361 taccacttgg tctctaacca gagcacgttc tcggttatga tcatcaagga aggtgattcg
      421 tactatacca tggccatgaa cagggaactg gaaaactcca tgttcacccg actctttttc
      481 ctccaagggg ctggtttaac tcgtttcagc cctatccatg cccaaccagg agttatcgtt
      541 tggaacgtgg ctgtgtaagt cacgttactc cactttttta ttttaaaaat ctttttatgt
      601 aaaaaaaagc aagctatatc taggatcacc taggtgatca tacagataaa ctaatcccca
      661 atctactttt tgagtgattt aaattgactt tagaagatag gatccgcagc attgaggagg
      721 agatcaaaaa aactccctac aacaaggcca cctcccacca cataggtaaa ttaaaggcca
      781 agttatccca gctacgagaa gaatcattta agagggcctc ggcaggtacc aagggaaagg
      841 gattccatct aaaaaaaagt ggtgactcca cagtggccct ggtagggttt ccttcagtgg
      901 gaaagtccac catacttaat cagataacta atgccgaatc taaaattggt gcctatgaat
      961 tcaccaccct ggaggttatc ccgggggtca tggagtatcg gggagcccat atccagatct
     1021 tcgatatccc ggggatcatc agtggcgcag cccatggccg gggacgggga agggaaatct
     1081 tatcagtggc tcgtaacgcc gatctggtcg tgatggttct ggatgttttc cagccacagc
     1141 accaggaaat gatactgcaa gaactggatc atattggtat ccggcctaac cagtcacctc
     1201 ctgatgtaag ggtcaagcgg aggaaagttg gtggaataaa attggcctcc acggtgcccc
     1261 tcacccacat ggatgaaaaa agcattcggt ccattcttaa tgagtacggg gtgcacagtg
     1321 ctgatgtcct gatccgggag gatgtaacca tggatagatt cattgactcc ttggattcca
     1381 gcatagtcta catacccctc ctggttgtgg ttaacaagat cgacttggta gacgcacatt
     1441 acttggaaga agttaaaaga aagcttccca atgctttgtt catcgccgct gataagcagc
     1501 taaatattga tgaacttaaa gaggagatct ttcagcgcct ggaactgata cgaatatact
     1561 taaaacctca gggtaaaaaa gctgatatgg aggaaccact cattgtccgg aagggttcca
     1621 ccgtggaaaa cgttgcccag aggcttcacc gagactttgt aaggaatttt cgccacgcca
     1681 atgtatgggg caaatcagtg aagttcccgg gccagaagat tggtctggaa cacgtggtgg
     1741 aagatgagga catagttagg atcatagtta aaaaataatt aaaaaggtgt gaacatgaag
     1801 ttagacgata taatcgtttc aaaggccatt gtggaaggtt acatggaaga cctgctgaat
     1861 tacatggaga tggacgtggc cattggtggt ggaggcccag ctggaatcac cgccggatac
     1921 tacctggcca aagccggatt aaaggtagcc ttattcgaaa aaaaactcac catcggtggt
     1981 ggtatgtggg gtgggggaat gatgtttaac aagatcgtgg tccaggaaga gggaaaacgg
     2041 atcctggagg agatggacat agtctcccag gagttccaac ccggatacca tgtggcggac
     2101 tccatcgaaa ctgtatccac cctcctgtcc agggcaacta aagcgggttt gaaggttttt
     2161 aacctcatcg aaattgagga tgtgatgatc aaacagcgag gagtagaagg actggttata
     2221 aactggagta ccgtgggcat gtctggactg cacgtggacc ccataacagt tcggagtaaa
     2281 gcagtggtgg atgccactgg acatccctgt gaggtggtta aggtagtgga agataaggtc
     2341 ggaccagaat tacacaccaa aactggcagg ataatgggag agaaatccat gtgggcggat
     2401 agggctgaaa gtatcatact ggaaaatgtg ggagaagtat accctggcct gtacgttgct
     2461 ggtatggctg ccaacgcagt ccatggttcg cctcgaatgg ggcccatatt cgggggcatg
     2521 ctactatctg gggaaagagt ggcccagata ctcattgaaa aactggaata gatatatcat
     2581 gatcctgctt ctaaccggca cccctggtac tggaaaaacc accatctccc gtttactgtc
     2641 ccaggaacta gaagccaagt tagtgctggt caaccagtta gtggaccaga aacacctcta
     2701 cactggtctg gatccggaga aaaactataa aatagttgat cttccgaatc ttttagggga
     2761 aatggacgag atcagtcaga attaccaggg cagtaaagat tggctaatat ttgagggaca
     2821 tttatcccat tactaccaac ctgcagatct ggtggtggtt ttaagggcca gcccatcaat
     2881 tttggaaaaa agactggaaa aacgtggatg gaaaccagaa aagataaaag aaaacctgga
     2941 agcagaggcc ctggatattt gtgcctggga agccggtgat attcatgggg cactggctca
     3001 ggaagtggac accacccact ccaggccagg ggatgtggtt aacaccatca tgggaatcat
     3061 caggggtgat ttaaccttac ccttgg
//
LOCUS       DAOQVW010000149         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_36981_length_3075_cov_4.636755, whole genome shotgun sequence.
ACCESSION   DAOQVW010000149 DAOQVW010000000
VERSION     DAOQVW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36981_length_3075_cov_4.636755"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKK85_04155"
     CDS             <1..342
                     /locus_tag="PKK85_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNS25242.1"
                     /translation="LAVDFGLPIIPTRSPTDTAAMIQRLAMREMEKGPRAIQTRTERK
                     PLSLEEQQLFIVESLPQVGPVTARKLLEEFGSVEGVVGASLKDLQKVEGIGKKIAQNI
                     RKVLKSVYKGQ"
     gene            387..1115
                     /locus_tag="PKK85_04160"
     CDS             387..1115
                     /locus_tag="PKK85_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019263103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNS25243.1"
                     /translation="MRILVDSRGKKFLADSNDLHTDAGYIKGEEIEKSSTGDVLMTHL
                     GKKYTVLDANINDYIELMERRCSIILPKDMGVMAAYTGIGCGNKVLDAGTGAGASAIF
                     LANLVGPNGKVYSYEIREDFAEIARRNIAGFGLNNVEVKCQDLALDLVEKDLDLVFLD
                     LPKPWILAEKAYSAIKTGGFLATYTPFVDQVITLHHVLKKVGFSNIETVECLLRGMEV
                     KSKGTRPKTRMTGHTGYLTFARKL"
     gene            1203..2117
                     /gene="pyrB"
                     /locus_tag="PKK85_04165"
     CDS             1203..2117
                     /gene="pyrB"
                     /locus_tag="PKK85_04165"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNS25244.1"
                     /translation="MVFSQGDIISIRDFSKDDIKYILRTAEKMEPIARSLEKSDILYG
                     KLLGMLFYEPSTRTRLSFEAAMKRLGGCTIGFSEARTSSATKGEKLSDTVRTVGEYTD
                     ALVLRHNMEGTARLAADIVDVPVINAGDGAGQHPTQTLLDLYTMKRLLGKIRNLHVAL
                     VGDLKYGRTVHSLAYALAMFGTRMSFVSPPELKMPREIMEDLNGAGVSVHETSAIRDV
                     LPYVDVLYVTRIQKERFPDPQEYLKIKGAYTIDKDLLKNSETIVMHPLPRLDEIATDV
                     DSTPYGKYFQQAFYGVPVRMALLKSLIK"
     gene            complement(2154..>3075)
                     /locus_tag="PKK85_04170"
     CDS             complement(2154..>3075)
                     /locus_tag="PKK85_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division control protein Cdc6"
                     /protein_id="HNS25245.1"
                     /translation="LEQVARDKNVNVRVEYIRCTDYTTEYQVIARLCQQMGQDVPYRG
                     WTKAEVIHAFRNLFKKNVFGKNLILIIILDEIDILLKNDGDGLLYTLTRTENVAIASI
                     SNYVEFKQFIKPRVKSSLRDREIVFPPYNAQQLVDILEERAKLSFNEKSLGNEVIPLC
                     AALAAREEGDARYALDLLRTSGELADEKGTEIVHEDYVREAKDNIEHNKVTDIVMTLP
                     SQQQKVLESLLYLTNHKEEITSGRLYDVYKDISKGDSVSYRRIFDFINELEMLGLIST
                     KTVSRGRGKGRTNIIDLQCDTHLLEEAIWQ"
BASE COUNT          909 a          634 c          692 g          840 t
ORIGIN      
        1 ctagccgtgg attttggtct gcccataatc cccaccagat cccccacaga caccgctgca
       61 atgatccagc gcctggccat gagggaaatg gaaaaggggc cccgggctat ccaaaccaga
      121 accgaacgca aacctctttc cctggaagaa cagcagctat tcatagttga atcactaccc
      181 caggtggggc cggttactgc tcggaagcta ctggaagaat tcggtagtgt ggaaggagtt
      241 gtcggagcta gccttaagga tctgcagaaa gtggaaggta ttggtaaaaa aattgcccaa
      301 aatatccgaa aagtccttaa atcagtttat aaaggccagt agattattta aacattagat
      361 taagaaattt ataaggaatt agtatcatgc gtattttagt agactccaga ggtaaaaaat
      421 ttctggcgga ttcaaatgac ttgcacaccg acgccggcta catcaaaggg gaggaaatag
      481 aaaaaagttc gacaggtgat gtgctgatga ctcacctggg aaagaaatac accgtccttg
      541 atgctaacat caacgattac atcgaattaa tggaaagacg ctgctccata atactaccta
      601 aggatatggg tgttatggcg gcttacactg gaattggatg tggaaataag gttctggatg
      661 cgggtactgg agcaggggcc agtgccatct tcctagccaa cttggtggga ccgaatggta
      721 aggtgtatag ttacgaaata agagaagact ttgcagagat agcccggcga aacatagctg
      781 gattcggttt aaataatgtg gaagtgaagt gccaggacct ggccctggat ttagtggaaa
      841 aggatctaga tttggtattt ttagaccttc ccaaaccctg gatactggct gaaaaggcct
      901 atagtgcaat taaaacaggc ggattccttg caacatacac tcccttcgtg gatcaagtta
      961 taaccctgca tcatgtactt aaaaaggtcg ggttttccaa tattgaaaca gtagaatgtc
     1021 tattaagagg tatggaagtt aaaagtaagg gcactcgtcc taaaactagg atgactggac
     1081 atactggata tttaaccttt gcccgtaagc tatgaaccat aacccacagt catattaaat
     1141 attataaata atcaacaaag agttaaaaga ttatgataaa atatgaatag ggggatcaat
     1201 gcatggtttt cagtcaaggg gatattattt ccatcaggga tttcagtaag gatgacataa
     1261 aatacatttt aagaaccgca gagaaaatgg agcccattgc tcggtccctg gaaaagtctg
     1321 atattttgta tggaaaactt ctgggcatgt tgttctacga gccctccacc cgtaccagat
     1381 tatccttcga agctgcaatg aaacgtctgg gaggatgtac cattggtttt tctgaagcca
     1441 ggacaagcag tgccactaag ggtgagaaac ttagcgacac tgtgcgaaca gttggtgaat
     1501 atactgatgc tcttgtactg agacataata tggaaggaac tgccagatta gccgcagata
     1561 tagttgatgt accagtgatt aatgctggag atggagctgg tcagcatccc actcagaccc
     1621 tgctggattt gtacaccatg aaacgactgc tgggtaagat ccggaatctt catgtggccc
     1681 ttgttgggga tctgaagtat ggtagaactg tgcattccct ggcctacgcc ctggccatgt
     1741 ttggaactcg gatgagcttt gtatctccac cagaattgaa gatgcccagg gaaatcatgg
     1801 aagatctcaa tggagccggg gtcagcgtcc atgaaaccag tgcaatcagg gatgttctac
     1861 cctacgtgga tgttctctac gtcaccagga ttcaaaaaga acgttttcca gatcctcagg
     1921 aatacttgaa gatcaagggg gcttacacca ttgataagga tcttttaaaa aatagtgaaa
     1981 ccatagttat gcaccccctg cccaggcttg atgaaatagc tacagatgta gacagcactc
     2041 cctatgggaa gtactttcaa caggcattct atggagtgcc agtccggatg gccctattaa
     2101 aatcactgat caaataaata aaataagtat tagatatttt tttgtacact atttcactgc
     2161 catatggcct cttccagaag atgggtgtcg cactgcaggt caattatgtt ggttcgtcct
     2221 ttaccccgtc cccgggatac tgtttttgta gatatcagtc ctaacatttc taattcgttt
     2281 ataaaatcaa atattctccg ataggataca gaatcacctt tactgatatc tttataaaca
     2341 tcgtagagtc tcccactggt aatttcctct ttatgattag tgagatatag tagagactcc
     2401 aggaccttct gttgctgact gggaagggtc ataactatat ctgtaacttt attatgttca
     2461 atgttgtcct ttgcttcacg aacatagtcc tcatgtacaa tttcagttcc cttctcatcg
     2521 gccagttcac cagatgttcg tagcagatcc aaagcataac gggcatcacc ctcttcacgg
     2581 gcagctaaag ctgcacagag aggtataact tcatttccca gtgatttctc attgaaagat
     2641 aatttagcac gttcttctag aatgtccacc agttgctggg cattgtaagg tggaaaaacg
     2701 atctcccgat cccgcaagct acttttcacc cggggcttga tgaattgttt aaattcaacg
     2761 tagttactta tagatgcgat ggcaacattt tcagtacgtg taagagtgta gagtaggcca
     2821 tcaccatcat ttttgaggag tatgtcaatt tcatccagga tgatgattaa gatcagattc
     2881 tttccaaata catttttttt gaaaagattg cggaaggcat gtatcacttc ggccttggtc
     2941 caacctcgat agggaacatc ctgccccatc tgctggcata aacgggcaat aacttgatat
     3001 tcagtagtgt aatcagtgca acggatgtat tctaccctga cattcacatt tttatcccgg
     3061 gccacttgtt ccaat
//
LOCUS       DAOQVW010000150         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_36982_length_3075_cov_4.515232, whole genome shotgun sequence.
ACCESSION   DAOQVW010000150 DAOQVW010000000
VERSION     DAOQVW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36982_length_3075_cov_4.515232"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKK85_04175"
     CDS             <1..419
                     /locus_tag="PKK85_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS25246.1"
                     /translation="KLSDKFDPGRLASLGMAIISMGLLIMAFLTEKTSLYVIIAGLAV
                     IGIGIGIFSAPNTHAIMGSVERKYFGVASATLSTMRLLGQTFGMGLILIVFAVYIGAV
                     QFTPQNYPALLTSIQVTFIISVLLSVVAIFASLARK"
     gene            complement(774..1301)
                     /locus_tag="PKK85_04180"
     CDS             complement(774..1301)
                     /locus_tag="PKK85_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNS25247.1"
                     /translation="MVYCEKCGTEILDKALYCSECGAKLKSDDDETANEGETLQEMIH
                     AAWNKGTVPPRKILCFTEENIYILEGTFLKGMGVGIGLVGLYLEKKDSSEKEEIAQTT
                     DFSKLAAQDPDVVIIPYQEIMKLNMGKKRMLLNPTITIKTTSEDYKFTVMEGKKYKQY
                     QKTLPALLGDKVTVE"
     gene            complement(1485..2759)
                     /locus_tag="PKK85_04185"
     CDS             complement(1485..2759)
                     /locus_tag="PKK85_04185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS25248.1"
                     /translation="METKNKELHNICMLSTHGYFDPVPELGKIDTGGQVLYVLNIARS
                     LAKDGINVDIYTRWFDPDKKQIETFPDTPNIRIIRIPAGGWEFIPKEKIYHVLPELAK
                     NMIGFINDNSLNYDLFHGHYVDGGIVALEMADAFSRPVYFTAHSLGAWKKQMVGGDPD
                     EMEQKYNFQHRISEELRIFQSVTGQTVTSHEEMAKIEELYHYKPKRVEFIPPGVDVHK
                     FRPLNPGEEEKQLDTKLPDRFIFMASRTSRAKGHDLLLKAYVDVLEEFKDVNLVFCGD
                     IHCQDLEGLKVLSNIKSIVEKNELKEHVKLLGCIPNDLLPVFYRKAEIYVLPARFEPF
                     GMAALEAMACQVPAVISQYASIQENLSNGEDYLKIDPYNTQELSNTIIKLLENEELAR
                     KIAKKGRETVVQNFSWEAVAQRFIEFYNQSTG"
BASE COUNT          830 a          673 c          595 g          977 t
ORIGIN      
        1 gcaaactatc tgataaattc gaccctggaa gattggcatc actgggaatg gccattataa
       61 gcatgggact cttgatcatg gcatttttaa cagaaaaaac cagtttatat gttataatag
      121 ctggcttagc tgtgatagga ataggaatcg ggatattctc cgcccccaac acccacgcca
      181 tcatgggatc cgtggaaaga aaatatttcg gagtggcatc agccacctta agcaccatga
      241 gactcctagg ccagaccttc gggatggggc tgatcctaat agtattcgcc gtttacattg
      301 gagccgtcca gttcactccc caaaactacc cagccctcct aaccagcata caggtaacct
      361 tcatcatttc cgtgctactg agtgtagtgg ctatctttgc atcattagct cggaagtgaa
      421 tggaaaatat ttaaagtttt ctctaattta ccaactctgg ataaaaagtt ctattaattt
      481 tttttattct tggaagacaa atttataaat aaatactgtg ttttttagaa aaatgaaaac
      541 ctgtaatcat aaatatggtt cttcaggaga gttatgtcct gaataagttt ggaaaaattc
      601 ggattattgc atattacaca ctgatttccc taaaaacagg aattcaccag attttaatat
      661 cttaactgaa gaaaaaaata aagaaattca aaagagggta ttgaacagga ttttgattat
      721 gaaggggtaa tgttatttag ttttgatttt tccaaccact cccctaccaa ttattattct
      781 acagtcacct tatcaccgag aagggcgggt agggttttct ggtattgttt gtattttttt
      841 ccctccatta ctgtgaactt gtaatcctcg gaggtggttt ttatggttat tgtggggttc
      901 agtagcattc ttttctttcc catattcagt ttcatgattt cctggtaagg aatgatcacc
      961 acgtctggat cctgggcagc taatttcgag aagtcggtgg tttgagctat ctcttccttt
     1021 tcggaggagt ctttcttttc cagatataag cccaccagcc ctattcccac ccccattccc
     1081 ttgagaaagg tcccttcaag gatgtagata ttttcttcag tgaagcagag tattttgcgg
     1141 ggcggcacag tacccttatt ccaggctgca tggatcattt cctgcagtgt ttcaccttca
     1201 ttagctgttt catcatcgtc cgattttagt tttgctccac attctgaaca gtaaagtgct
     1261 ttatctaaaa tctcagtccc acatttttca cagtacacca tcttactaac tcctaggatt
     1321 tgtatcagat ataattgtgt tagtagatat atgcattcct caacaattct ttttgtaata
     1381 tttgctcagg accttaccaa agatagcctc tcctacttaa aacaatccct tcccctggta
     1441 ctttatcatc cggatgatta atgatcgtcc ggctctaatg ctttttaacc cgtggattgg
     1501 ttataaaatt ctataaatct ttgggcaaca gcttcccagc tgaaattttg caccactgtt
     1561 tctcgtccct tctttgctat tttccgggcg agttcttcat tttccaggag ttttattatg
     1621 gtgttggata gttcctgagt gttgtagggg tcgattttca ggtagtcctc gccgtttgat
     1681 aggttttcct gtatacttgc atattgggag atgacagctg gcacctggca ggccatggct
     1741 tccagggcag ccatgccaaa gggttcaaac ctggctggta agacatatat ttctgctttc
     1801 ctgtaaaaaa caggcagtag atcgttgggt atgcatccta acaatttcac atgttccttc
     1861 agctcatttt tctccactat gctcttaata tttgatagaa cctttaatcc ctccaggtcc
     1921 tggcaatgga tatctccaca aaaaacaaga ttcacgtctt taaattcctc caggacatct
     1981 acgtatgctt ttaggagtaa gtcatgtccc ttagcccggg aggtgcggct ggccatgaat
     2041 ataaatctat ctggaagctt agtatcaagt tgtttttcct cttcaccggg attaaggggc
     2101 cggaacttgt gcacatccac accgggaggg atgaactcca cccttttagg tttgtaatgg
     2161 tacaactctt ctatcttagc catctcttca tgactggtaa ctgtctggcc tgtaaccgac
     2221 tggaatatgc gcagctcctc agaaatcctg tgctggaagt tatacttctg ctccatctca
     2281 tctgggtctc cccccaccat ctgtttcttc caggccccca gggagtgggc agtgaagtag
     2341 acgggcctgg aaaaagcatc agccatctct aaggccacaa tacccccatc cacgtaatgt
     2401 ccatggaaaa gatcataatt taagctatta tcattgataa aaccaatcat atttttggct
     2461 agttcaggta gtacatggta tattttttct ttggggatga actcccatcc tccggcgggt
     2521 atgcggatga tcctaatgtt gggggtgtcg gggaaggttt caatttgttt tttatcagga
     2581 tcgaaccagc gggtgtaaat atccacattg ataccatctt tagccagtga acgagcaatg
     2641 ttcaggacgt agagaacctg tccacccgtg tcgattttac ccaactccgg gaccggatca
     2701 aaatacccat gagtactcag catacaaata ttatggagtt ctttattttt cgtttccatg
     2761 gtcaatcctc ctttaaattg aatcaatcct aatattgtat aagtaaatta aagtttaaga
     2821 acttaactaa aaaaagaaat cttaaaacca gcaatagaat cagaactatt atctactctc
     2881 ccaaaaaagt gtcaagagac atgtaagaaa gagattaact ggatacttct agctggcctc
     2941 ttcagtatat agtgaggaag cttgacatct aaaaggagta acatcagctt tccaggtccc
     3001 tgtcataaaa atccattaac ctttgggcaa cagcttccca gctgaaattt tgcaccactg
     3061 tttctcgtcc cttct
//
LOCUS       DAOQVW010000151         3057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_37298_length_3057_cov_4.265823, whole genome shotgun sequence.
ACCESSION   DAOQVW010000151 DAOQVW010000000
VERSION     DAOQVW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37298_length_3057_cov_4.265823"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>3057
                     /gene="ileS"
                     /locus_tag="PKK85_04190"
     CDS             52..>3057
                     /gene="ileS"
                     /locus_tag="PKK85_04190"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNS25249.1"
                     /translation="MATEEAQRSYNPQIIEKRAQEFWEARKTYQMSQKNREDGPRYSF
                     LDGPPYCSGRIHLGTAWNKIIKDTYLRYKTMAGCNVRRQAGWDAHGLPIEHKVEGLLG
                     LKSKKEIETKIGIENFVEKCREFAIENQGIMTQQFKMLGVWMDWDQPYITFDPKYMES
                     CWWTLKRAHEKDLLVQDLRVITWCPRCETALALAEIDYEDKEDPSIYVKFSLKEVQPG
                     LSENTYILVWTTTPWTLPANMAICVHPDYDYAYVKVGEETYLLAEAMVDSIFGELEYE
                     IIRVVKGSQIEGLEYQHPLSQEIPVQNDFKHVILPGEHVTLTEGTGCVHTAPGHGPDD
                     FEIGKAYGLPIFCPVDEAGLFKEEAGKYHGQFVKDADPYIITDLDTHGLLFKEGTIKH
                     RYGFCWRCKTPIIYLATEQWFLKITQIKNQLLSELDKVEWVPSWAGESRFRNWVENAR
                     DWTISRQRYWGIPIPIWTCSDCDEITVVGSLEELVQLSQEKLEGDVLHRPYVDQVKIP
                     CHCGGQMQRTPDVLDVWIDSGVAGWAALYYPKEQKLFEEWYPYDFITEGHDQTRGWFY
                     SQLGCGMIALDSVPYRKVLMHGFTLDEEGKKMSKSLGNVVEPDEVVAKYGADVLRFYL
                     LWANKPWEDLKFNWDEVKNINKMFNILWNVFIFASTYMGLDNFQPTKYGAENVKFRVE
                     DRWITSRVQSVAQEVTKSLEALHLHQATRSLQDFILEDLSRWYVRLIRGRTWIEKDDP
                     DKLGAYQTLYTTLKSVVTLMAPLSPHICEEIYQHLIRPLETYLPESVHLLDWEVDETL
                     RDQKLEADMNVARDIIEACARARDSARYKLRWPVQEIVIVSEDQGILEASQNLEDVLL
                     EQANTKKITPSQEFQDLKIRVLPNMKTLGPRLREDVPRVAAKLSEVDGHELMEKMDAE
                     GIFVVKLDDVNVELRPDDVVLETELPDNVTNSEFPGGNVFVDTQITPEILSEAMSREL
                     VRRIQDMRKDLDLEVEAHIQVNVDCSAEFQ"
BASE COUNT          848 a          702 c          804 g          703 t
ORIGIN      
        1 caaagctagt tttgtataaa tcatccaaga tcattaacga ggagaataat tatggcgact
       61 gaggaagccc aacgttctta taatccacaa atcatagaaa aaagagcaca ggagttctgg
      121 gaggctagga aaacctacca gatgagccag aaaaatcggg aggacggacc ccgttactcc
      181 ttcctggacg gaccacccta ctgcagtggc cgaatacacc taggaactgc ctggaataag
      241 attataaaag atacatatct ccgttataag accatggccg ggtgtaacgt gcgcagacag
      301 gctggatggg atgcccatgg cctccccatc gaacataaag tagaaggtct cttgggactc
      361 aaaagcaaaa aggaaatcga aactaagatt ggtatcgaaa atttcgtgga aaagtgtcgg
      421 gaattcgcca tagaaaatca gggaattatg acccagcaat tcaagatgct gggagtgtgg
      481 atggattggg atcaacccta catcaccttt gacccgaagt acatggaatc ctgttggtgg
      541 accctgaaaa gagcccatga aaaggatctg ctggtacagg acctccgggt cattacctgg
      601 tgccctcgct gtgaaactgc cctggccctg gcggagatcg attacgaaga caaagaagac
      661 ccctccatct acgttaaatt ttctctgaaa gaagttcaac ccgggttatc agaaaatacc
      721 tacatcctgg tttggaccac cacaccctgg acccttcctg ccaacatggc catatgtgtt
      781 catcccgatt atgattacgc ctacgtaaag gttggtgagg aaacatatct gctggcagag
      841 gccatggtag actccatttt tggggaactt gaatatgaaa taatcagagt agtgaaaggg
      901 tctcaaattg agggcctgga ataccagcac cccctgagcc aggaaatacc cgttcagaat
      961 gatttcaaac acgtaatact gcccggtgaa catgtgaccc tgactgaagg tactggttgc
     1021 gtgcacactg ctccagggca tgggcccgac gattttgaga ttggtaaagc ttatgggctg
     1081 cctatattct gtcccgtgga tgaggctgga ttgttcaaag aagaagctgg taagtaccat
     1141 ggtcagtttg ttaaggatgc agatccttac atcatcaccg acctggatac ccatggtcta
     1201 ctatttaaag aaggcacaat taaacaccgt tatggattct gctggagatg taaaactccc
     1261 atcatctact tggccacgga gcagtggttt ttgaagatca cccagatcaa gaaccagctc
     1321 ctcagtgaac tggataaggt ggaatgggtt ccttcctggg ctggtgagag ccgcttccga
     1381 aactgggtcg agaacgcccg ggattggacc attagtcgcc agcgctactg gggcataccc
     1441 atacccatct ggacttgtag tgactgtgac gagattactg tggtgggatc ccttgaggaa
     1501 ctggtccaat tatcccagga gaaactggag ggcgatgtac tgcaccggcc ctacgtggat
     1561 caggtgaaga taccttgcca ctgtggaggc cagatgcaac gtacacccga tgtactggat
     1621 gtgtggatag actctggtgt ggctggatgg gctgctcttt actatcctaa ggaacagaag
     1681 ctatttgaag agtggtaccc ctatgatttc attactgaag gccacgacca aaccagggga
     1741 tggttttact cccagttggg ctgtggaatg atcgccctgg acagtgtccc ctaccgtaag
     1801 gtccttatgc acggattcac cctggatgaa gagggtaaaa agatgagcaa atccctgggt
     1861 aatgtggtgg aacccgatga agtggtggct aagtatgggg ctgatgtgct gcgtttctat
     1921 ttgctctggg ccaacaaacc ctgggaggat ctgaagttca actgggatga ggtgaaaaac
     1981 atcaacaaga tgttcaacat cctctggaat gttttcatat tcgcatccac ctacatgggg
     2041 ctggataact tccaacccac caaatacgga gccgagaatg ttaaattccg ggttgaagat
     2101 cgttggatta cttcccgggt acaatcggtg gcccaagaag tcacgaaatc ccttgaagct
     2161 ctgcatctgc accaggccac tcgcagcttg caggacttta tactggagga tctcagccgc
     2221 tggtacgttc gccttattcg tggtcggacc tggatcgaga aagatgaccc ggacaaactt
     2281 ggggcctacc aaacccttta caccacactt aagagcgtgg taaccctcat ggcaccccta
     2341 tccccccaca tctgtgagga aatctaccag caccttatca ggcccctgga aacatatctc
     2401 cccgagagtg tgcatctttt agactgggaa gtagatgaaa ctctccgaga ccagaagtta
     2461 gaggctgata tgaacgtggc acgggatatt atagaggcct gtgcccgggc ccgggactcc
     2521 gcaaggtaca aactacgctg gccagtgcaa gagattgtga tcgtgtccga agaccaggga
     2581 attctggaag cctcccaaaa tttagaggat gtgttgctgg aacaggccaa cactaagaag
     2641 atcactccat ctcaggaatt ccaggacctt aagataaggg tcttgccaaa tatgaagacc
     2701 ctggggcccc gtctgcggga agatgttccc cgggtagcag ccaaactgtc cgaggtagat
     2761 ggtcatgaat taatggaaaa aatggatgct gaagggattt ttgttgtaaa actggatgat
     2821 gtaaatgtgg agctaaggcc agacgatgtg gtgctggaaa ctgaactgcc ggataacgtc
     2881 accaactccg aatttccagg gggtaacgtt tttgtagata cccagatcac ccctgaaatt
     2941 ttatcagaag ccatgagccg ggaattggtg cggaggatac aggatatgag gaaggatctg
     3001 gacttggagg tggaggccca tatccaagta aatgtggatt gcagtgccga gttccag
//
LOCUS       DAOQVW010000152         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_37623_length_3040_cov_2.666332, whole genome shotgun sequence.
ACCESSION   DAOQVW010000152 DAOQVW010000000
VERSION     DAOQVW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37623_length_3040_cov_2.666332"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PKK85_04195"
     CDS             complement(<1..1224)
                     /locus_tag="PKK85_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS25250.1"
                     /translation="MITRQEKKYSDKEIYNILHPWVREWFQGNFETFSQAQRQAIVDI
                     HQGQNVLVSSPTGSGKTLTAFLSIISELTTLAEENKLKDEVYCIYISPLKALDNDIEK
                     NLEDPLRGIEELAGKKLGIRKMVRTGDTSQYQRSKMLKTPPHILITTPETLSILLVAP
                     KFREKLKSVKYVIVDEIHSLADNKRGTHLSLSLERLARMVGDFTRIGLSATVHPLERV
                     AEFLVGLHYGQPRNCLIVEVNYLKQLDMELICPVEDIITTETDEVNRAMYQSLHELIQ
                     EHQTTLIFTNTRSGTESVVFNLKKRFPDSYNHDNVMAHHSSLSRELRLEAENQLKDGK
                     LKVVVSSTSLELGIDIGYIDLVVLVSSPKSVSRALQRIGRSGHQLHEKSKGRMMVVDR
                     DDLVECALILKNARDG"
     gene            complement(1272..2012)
                     /locus_tag="PKK85_04200"
     CDS             complement(1272..2012)
                     /locus_tag="PKK85_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS25251.1"
                     /translation="MVNISLYGARIVDLALMANDYLVISDLHLGYEEALNYQGVMVPK
                     FQYPLLTNRLEEVLFQQKAEGIIINGDFKHEFGKISRQEWSETLDFVDFLRNHFQEII
                     LIQGNHDPLTPIIARKTGLEMHKHYHTGDFLVMHGDQVPGNWDEIDEKTIIIGHEHPT
                     LGLRSGERVEKMKCYLKGQFQGKNLVVMPSFNFVSEGSDILHEKPLSPFLKGDIKDWE
                     VYGVEDFEILYFGQVKDLLEFQKRYNMI"
BASE COUNT          750 a          705 c          675 g          910 t
ORIGIN      
        1 gccgtctcgg gcatttttaa ggatgagggc gcactcaacc aggtcgtccc tgtccaccac
       61 catcatacgt cccttgctct tctcatgcaa ctggtggccg ctgcgaccta tacgctgcag
      121 ggcccggctc actgacttgg gggagctcac cagcaccacc aggtctatgt agccgatatc
      181 aatacccaac tccaatgaag tggaagaaac caccaccttc aacttaccat cctttaactg
      241 gttctcagcc tccaggcgca gctcccggct cagggaggaa tggtgggcca tgacattatc
      301 atggttataa ctatctggga agcgcttttt gaggttgaac accacactct ctgttccgct
      361 gcgggtgttg gtgaagatga gggtggtctg gtgctcctgg atgagttcat gcagggactg
      421 gtacatggcc cggttcactt catcggtttc ggtggtgatt atatcctcca ctgggcatat
      481 aagttccata tccagttgtt taaggtagtt aacctccact atgaggcagt tacggggctg
      541 gccatagtgc aggcccacca ggaactcggc caccctttct aatggatgta cggtggcgga
      601 aagtccgata cgggtgaaat ctcccaccat cctggccaac cgttctaatg agagggagag
      661 atgggtgccc cgcttgttat cagccaggct gtggatctca tccacgatga cgtacttcac
      721 actcttaagc ttctcccgga acttgggagc caccaggagg atggaaagcg tctcaggagt
      781 tgttatcaag atatgtggtg gggttttgag catcttggag cgctggtact ggctggtgtc
      841 tccggtacga accatcttcc ggatgcccag cttcttcccg gcaagctctt caatccccct
      901 cagaggatcc tccaggttct tctcgatatc attatccagg gccttcaggg gggagatgta
      961 gatacagtac acctcatctt tcagtttgtt ctcctcagcc agggtggtca gctcactgat
     1021 gatggataag aaggccgtca gggttttacc cgaaccagtg ggggaggata ccagcacatt
     1081 ctggccttga tggatatcca cgatggcctg gcgctgggcc tgggaaaagg tctcgaagtt
     1141 tccctggaac cattcccgca cccagggatg gagtatattg taaatctctt tatctgagta
     1201 ttttttttcc tgtctggtga tcatgtgctc ttaacttccc aaatataagt tcttaacaat
     1261 ctctaatcta cttaaatcat gttgtaacgc ttctgaaact ccaataaatc cttaacctgg
     1321 ccaaagtaga ggatttcaaa gtcctccact ccgtaaactt cccagtcctt aatatctccc
     1381 ttgagaaacg gggaaagagg tttctcgtgg aggatgtctg aaccttcact tacaaagtta
     1441 aaggacggca tcaccaccag attcttcccc tggaattggc ctttaaggta acatttcatc
     1501 ttctcaaccc tctccccact ccggagaccc agagtggggt gttcatggcc gatgatgatg
     1561 gtcttctcat ctatctcatc ccagtttccg ggtacctggt ccccatgcat caccaggaaa
     1621 tccccggtgt ggtagtgttt atgcatctcc agaccagttt tccgggcgat gatgggtgtc
     1681 agggggtcat ggttgccctg gataaggatg atctcctgga agtgattcct taagaagtcc
     1741 acgaagtcca gggtttcact ccactcctgg cggctaatct tcccaaattc atgtttaaaa
     1801 tcgccattga taataatgcc ctctgctttt tgctggaaga gcacctcttc caggcggttg
     1861 gtcaggaggg ggtactggaa ttttgggacc atcactccct gatagttcaa tgcttcctcg
     1921 tatcccaggt gtaaatcgga gataaccaag taatcattgg ccataagggc caggtccact
     1981 atccgggcac cgtaaagact gatatttacc ataatttttt aatccctgta tagaatttca
     2041 taaaaatatt tacaatagct aaagattggt ggggtcttgt ggattaatac taattggagt
     2101 aagacttttc tatatgaaat tccttttccg atattatata attccttcct ggactacttt
     2161 ccagggtcta cgttgacata ttctaagggt agaacaagtt ttttgtaaaa tttaatttat
     2221 aagttttttc ccatctcata ggcttctgtc atggcttcac ttcccttgat gtctcccatg
     2281 ttctgggcac cgcttccata gatcacaccc atttctttac ccactaaaga cgaggtaacc
     2341 tcctgaatct ctctactctc ctttccagtg cctatttatt cctggcagca ggagtggttt
     2401 agcacggctt ttttatctgt attaccaggt ttatgcatag aactgaacta cttttaactg
     2461 cacacctctg cctaaatctg caggttaaat ataaaataca cgatatagaa gcagtaaatt
     2521 gatgtttaga gcttcctatt ttattgtata ctacaacaga tgaatcagtg tatggaatgg
     2581 tatcccatat gtgattcttc atctgttgct tctaacttga aaataattgt attataagag
     2641 ggggattatc ttatcataga aattatgctg tgttgtcaat tgttttgggg cgctgccaat
     2701 tatcgcaact tactacgaaa attattttag ttctaaggtg catgttcttt gtatatccac
     2761 agcctggttt ctaatacaat attgtcagta ataagcattc tttttatagt aaggactatc
     2821 atatatctat acagacttga atcccttaca ccaaggaatc tatctgatga tatgttctga
     2881 tggttggttg gggttttggt aacagaaccc atttaagatt agggacaatt aatattaaca
     2941 agaaaaaaga ggtgaaaata attgaaaaaa catctatcaa taatcgtagt ggcatttttg
     3001 gtgggagtga tcatctgtgg ttctgtttct gcagctgatc
//
LOCUS       DAOQVW010000153         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_37986_length_3020_cov_3.252277, whole genome shotgun sequence.
ACCESSION   DAOQVW010000153 DAOQVW010000000
VERSION     DAOQVW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37986_length_3020_cov_3.252277"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKK85_04205"
     CDS             complement(<1..320)
                     /locus_tag="PKK85_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS25252.1"
                     /translation="MLLQITDLAVEVNNKEILSDVDLYIGKGETHVLLGPNGSGKSTL
                     FMTLLGFPKYKITRGEIIFKGEDVTHLSTTHRVKIKMKMSFQNPPTIRGVKLGDLLKI
                     EHGSG"
     gene            850..1287
                     /locus_tag="PKK85_04210"
     CDS             850..1287
                     /locus_tag="PKK85_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNS25253.1"
                     /translation="MAEDDVKIVMFCCNWCSYGGADTAGTARMQYPPNVRVIRVMCSG
                     RIEPQFVLKAFREGADGVIVAGCHHGDCHYDAGNYKLDRRMRIIYKLADDLGIGRERI
                     HHDWISASEGEKFADTVKMMVDRIQGLGPSPLKAQLEAEEVEA"
     gene            1289..2218
                     /locus_tag="PKK85_04215"
     CDS             1289..2218
                     /locus_tag="PKK85_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HNS25254.1"
                     /translation="MTEKAKIATMWLSGCSGCHLAFADIHEAIIDVMGLADIEFTPVL
                     MDVKYDEVPDDLDAIIIEGGIRNDENRELAEMLRKKAKIVIAYGTCAAYGGIPGLGNL
                     HAPEDLIQEAYLNSPSTPNPDNVIPSEEIPHLESRVRPLGEVIDVDLALPGCPPKSDI
                     VAQVLVALLTGEPIELPSTNLCEVCPREKPPEGMAMDKILRQFELGEPEEEMCLVPQG
                     LVCLGPATISLCGAECPSIGIQCRGCYGPTPKVTDQGAKMISAIGSDFGVERDKTVDP
                     EEVADQLDDVVGTFYTYTLPAALVPAKVKKEGK"
     gene            2218..>3020
                     /locus_tag="PKK85_04220"
     CDS             2218..>3020
                     /locus_tag="PKK85_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNS25255.1"
                     /translation="MVKLTMEPVTRIEGHAKITVDLDDAGNVQDTKLHVMEFRGFEKF
                     LQGRNIEEVPRIVPRICGICDVQHHLAAAKAVDEIFGFSSEEILPTAYRMREIMNWGS
                     TMHSHALHFYFLAAPDFIAGKDRKTRNVFQIIKDAPDVALQAIELRKNALSIIKAIGG
                     RPIHPTSSTPGGISTSLDDETQKDLLKKAQRNVELAVATLDLAKPIFEENLDLVKTLG
                     YIESYHCGLVKDGVWDMYDGKVRMKDKEGKLYAEFKNTEYLDYMAEHVKP"
BASE COUNT          954 a          623 c          677 g          766 t
ORIGIN      
        1 ccactaccat gttcgatttt aagtaaatca cctagtttaa ctccccgtat tgtaggtggg
       61 ttttggaaac tcattttcat ttttatttta actcgatgag tagttgatag gtgggtcaca
      121 tcctctcctt tgaagattat ttctccccgg gtaattttgt acttggggaa tccaagaaga
      181 gtcataaaaa gggtactttt accagacccg ttaggaccta aaagaacgtg agtctctcct
      241 ttgccaatat aaaggtctac atcactaaga atttctttat tatttacttc tactgccagg
      301 tcggttattt gaagtaacaa gtttaaccac ctctctggtc attttttatg gaccgatcag
      361 gggttcagca acgcccaatc agtactaggt tttattgagg tattggcata aataaataac
      421 taaaaaggag aaaatcaaga ataaaggtgt ttatcaaaaa taagggccat tttttgaata
      481 taaactgttt gttaatcata ttaatataga atataagatt aaaaataaat aaacactgac
      541 agttcctttt tataggcttc taaatcatat ttaaacttca acaaaactgt taaacatatt
      601 tctgactgta tttctggctt atgggaataa ttggtgctta agacaaaaaa ccataaatag
      661 tcaactagtc acatgttaag atataagaag taagtaatat atctcacacc ccgggtggta
      721 ataataaaaa actcctacgt attcgtacat atatattttt cgaattgatt ttctaagtaa
      781 gctttataat aactatatct taactcatat acgtacaata ggtacacaat aaatcaagga
      841 ggaagccata tggctgagga tgatgtgaag attgtgatgt tttgttgtaa ctggtgctcc
      901 tacggtggtg ctgataccgc tggaaccgca aggatgcagt atcctccaaa tgtaagggtt
      961 ataagggtaa tgtgttctgg aagaatcgaa cctcagttcg ttcttaaggc attcagggaa
     1021 ggagctgacg gagttatcgt cgcaggctgc caccacggag actgccacta cgatgcagga
     1081 aactacaaac tagaccgcag aatgagaatt atatacaaac tagcagatga tctaggaatc
     1141 ggaagggaaa ggattcacca cgactggatc tctgcatctg aaggcgaaaa attcgcagat
     1201 actgttaaaa tgatggttga ccgaatacaa ggcttgggtc catccccact aaaagctcag
     1261 ttagaagcag aagaagtgga ggcataaaat gacggaaaaa gcaaaaatag caacaatgtg
     1321 gctcagtggt tgctccggtt gccacctggc cttcgctgac atccacgagg ccatcataga
     1381 tgtgatgggc ctggcggaca tagaattcac cccggttctc atggacgtca agtacgatga
     1441 agttccagat gatctggatg caattattat tgaaggcgga atccgaaacg acgaaaaccg
     1501 agaattggct gagatgcttc gtaaaaaggc taaaatcgta attgcctacg gaacttgtgc
     1561 agcttacggt ggaattcccg gtctgggaaa cctacacgca cccgaggatc ttatacagga
     1621 agcttacctg aattcaccca gcacccccaa cccggataat gtcataccca gtgaggaaat
     1681 tcctcaccta gagagtaggg taagaccact gggggaagtg atcgatgtgg atctagcctt
     1741 accaggttgt ccaccaaaat cagatatagt agcccaagta ttagtggctc tgttaactgg
     1801 tgaaccaata gaactgccca gcaccaacct ctgtgaggtt tgcccacgtg aaaaaccacc
     1861 agaaggcatg gccatggaca agatccttcg ccagtttgaa ctgggagaac cagaagaaga
     1921 aatgtgtcta gttccccagg gattggtatg tctcggtcca gctactatct ccctctgcgg
     1981 agctgaatgt ccaagcatag gcatccagtg cagaggatgt tatggtccca ctcctaaagt
     2041 aacagaccag ggagccaaaa tgatcagtgc cattggttcc gacttcggag tagaaaggga
     2101 taaaacagta gaccctgaag aggtcgccga ccaattagat gatgtagtcg gcacattcta
     2161 tacttacaca cttcctgcgg ctcttgtacc cgctaaagtg aaaaaggagg gtaaataatg
     2221 gttaaattaa ctatggaacc cgtgacccgt attgaaggac acgcaaaaat cactgtggat
     2281 ctggacgacg ccggaaacgt ccaggacacc aagctccatg ttatggaatt ccggggattc
     2341 gaaaaattcc tgcaaggacg aaatattgaa gaagtaccaa gaattgtacc ccgtatatgc
     2401 ggtatctgtg acgtgcaaca ccacctggca gctgcaaagg ctgtggatga gatatttgga
     2461 ttttcatctg aagaaattct tcccaccgcc tatcggatga gggaaatcat gaactggggt
     2521 tccaccatgc actcccacgc cctgcatttc tacttcctgg ctgcaccaga cttcatagcc
     2581 ggtaaagaca ggaaaaccag gaacgtattc cagatcataa aggacgcacc ggatgtggca
     2641 ttacaagcta ttgaactacg aaaaaatgct ttgagcataa taaaggctat agggggacga
     2701 cctatacacc ccacctcatc cacaccgggt ggaatatcca ccagcctgga cgatgaaacc
     2761 cagaaggatc tcctgaaaaa ggctcagagg aatgttgaac tggctgttgc tactttagac
     2821 ttggcaaaac ctatcttcga agaaaacttg gacctggtca agactctagg ttacattgag
     2881 agctaccatt gtggcctggt taaagacggc gtgtgggata tgtacgatgg aaaagtgcgt
     2941 atgaaagata aagaaggtaa attgtacgct gaattcaaaa atactgaata cttggattac
     3001 atggccgaac atgtcaaacc
//
LOCUS       DAOQVW010000154         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_38295_length_3004_cov_3.838250, whole genome shotgun sequence.
ACCESSION   DAOQVW010000154 DAOQVW010000000
VERSION     DAOQVW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38295_length_3004_cov_3.838250"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKK85_04225"
     CDS             complement(<1..249)
                     /locus_tag="PKK85_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNS25256.1"
                     /translation="MPPEKLKIIGTAHVSQLSVEEVKTAILEDEPDVVAVELDPNRYY
                     NLMNQKEGVAEESKKFKMGEIIKGNNLTLFLVSAFLSYF"
     gene            complement(255..926)
                     /gene="comB"
                     /locus_tag="PKK85_04230"
     CDS             complement(255..926)
                     /gene="comB"
                     /locus_tag="PKK85_04230"
                     /EC_number="3.1.3.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNS25257.1"
                     /translation="MDVSLTLENSYSEDVCIMVDVLRASTTITVALEKFSEVIPVTEL
                     EEALNLASQKEVVVAGERGGATVKGFDTGNSPVEIQRFCGETLILTTSNGTRILGSMK
                     GTPLIGSFINAESVALKALELAQDHIEVVMAGVNGRFAIEDFLGAGIIISHLQRSCTL
                     DEMALASVLACQDIGKVEKSIYKSLSAANLCELGFKKDVEFCLQQDISELVPVYQDGI
                     IRSQM"
     gene            complement(977..1186)
                     /locus_tag="PKK85_04235"
     CDS             complement(977..1186)
                     /locus_tag="PKK85_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNS25258.1"
                     /translation="MYDEDETQQTAPIAEGEEYEVKIEDLGKEGDGITRVQGFVVFVP
                     DTKIGDEIKIRITSVRRRFAFAEKI"
     gene            1418..1939
                     /locus_tag="PKK85_04240"
     CDS             1418..1939
                     /locus_tag="PKK85_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25259.1"
                     /translation="MKVVTTPMCQEIVILAGVSDFIVSTDPDSTDADLAVVLWETKTT
                     KPSFRVKLNTFSQIIDSIRALADEVGTHNHKLNLDHYVPPKDEERKKIKVKVYSNFLK
                     DIVVDMGFSLVEDEADFLVYPDYFRKKISDEIEVAGERAIEIPSHKNAPVDPLKRTHL
                     RYNILEERLCTRL"
     gene            1924..2877
                     /locus_tag="PKK85_04245"
     CDS             1924..2877
                     /locus_tag="PKK85_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HNS25260.1"
                     /translation="MYETLTYTGGVHKHEEMNELIEDLGGFVLQETVSQMDLVLTLAI
                     PMEDLEKVQEKAKELLGKVRLAPMAGTELAIVSPTLARHHLPHSACDISEYLRRFGAK
                     DNMIGLRRGAGKGISHISEEEKRLIEEHDLAVFALGSFRECILKKTHLFEDLEIPIVV
                     TGAPEIDVEGIPGADAYVSGLGRIPRRLKRGENIRALKKLAEVVEKIIDNRRIDMSDD
                     PPIVPSILVKTEIENQVPAIKKVYSPAPVVSQLDGVRVKLDYDQYHEEIENLMVQDYR
                     LGDIADIKRSFMYNYILVKLLSETSLVEPLEQEISQKATAK"
BASE COUNT          893 a          616 c          621 g          874 t
ORIGIN      
        1 aaaataggat aagaaggcac tcaccaaaaa tagggttaaa ttatttcctt taataatttc
       61 acccatttta aatttcttac tttcttcagc aaccccttct ttttggttca tcaggttata
      121 gtaacgattg ggatcaagct ccaccgccac tacatctggt tcatcctcca gtatagctgt
      181 tttaacttcc tctacgctga gctgggatac atgggcggtt ccaatgattt taagtttttc
      241 tggcggcatt tttttcacat ctgggatctg atgatcccgt cctggtaaac cggaactaac
      301 tctgaaatgt cctgttgcag acagaattca acatcctttt taaaacccaa ctcgcagaga
      361 ttagctgcag ataaggattt gtatattgat ttttctactt ttccaatatc ctgacaggct
      421 aataccgaag ccagtgccat ttcatccaat gtgcagcttc gttgcaggtg agaaattatt
      481 atccccgcac ctagaaagtc ttcgatagcg aatctaccat tcacaccagc cataactact
      541 tcgatatggt cctgtgctaa ttccagagcc ttcagtgcca cagattcggc gttaataaat
      601 gatcctatca ggggagtgcc cttcatactt cccagtatgc gggttccatt gctggtggtc
      661 aggatcaaag tttccccaca gaatctctgg atttctactg gtgaattccc tgtgtcaaac
      721 cctttcacag ttgctccccc cctttctcct gccaccacaa cctctttttg ggaggccaag
      781 ttcagggcct cctctaattc ggtaactggt atgacttctg aaaatttttc cagagctacc
      841 gtgatggtgg tactggcccg aagcacatcc accattatac aaacatcttc agaataagaa
      901 ttctccaagg ttagggaaac atccatggaa acaccaaaaa tagaaagtat tataggtaaa
      961 aaaataggtt caatgtttat attttctcag caaaagcaaa tcgtcttcta actgaagtga
     1021 ttcggatttt tatttcgtca ccgattttgg tatctggtac gaagaccaca aaaccttgaa
     1081 cacgagttat tccatcgcct tccttaccta aatcttcaat tttaacttca tattcttcgc
     1141 cctcggctat gggcgcggtt tgttgagttt catcttcatc gtacaattca aatcacatcc
     1201 tgaagaccaa gattatctgg tcatagataa gcactgatct ttctattatt gtatataaat
     1261 attgttgtaa aggtgtctaa atgccattat tccttgttta taggtcataa aaggcttgtt
     1321 ttctcatgaa ttcaaacata gatattattc attagttacc gttataatcc cattaacaca
     1381 ctatataata tataagagtg ctgccagttg atctatcatg aaagttgtca ccacacccat
     1441 gtgccaggag atcgtaattc tggctggagt ttctgatttt atagtctcca ctgatcctga
     1501 ttccactgat gctgatctgg ctgtggtatt atgggaaact aaaaccacca aaccatcctt
     1561 cagagtcaaa ttaaatactt tctctcagat tatagacagt attcgggccc tggctgatga
     1621 agtgggtaca cataatcata aattaaactt ggaccattac gttccaccta aggatgagga
     1681 aaggaaaaaa ataaaagtta aggtttactc aaatttcctt aaagatatcg tggttgacat
     1741 gggcttcagc ctggtggagg atgaggctga ttttctggtg tatcctgatt attttcgtaa
     1801 aaaaatatct gatgaaattg aagttgcagg ggagagggcg attgaaattc cttcccataa
     1861 gaatgcgcct gttgatcctc tgaaaagaac tcatctcagg tacaatatcc tggaggaaag
     1921 attatgtacg agactttaac ttacacaggc ggagtacaca agcacgaaga gatgaatgaa
     1981 ctcattgaag atcttggtgg ttttgtactg caggaaaccg tcagtcaaat ggatctggtc
     2041 ttaaccctgg caattcctat ggaagatttg gaaaaagtcc aggaaaaggc aaaagagttg
     2101 ttggggaaag taaggctggc tcctatggcc ggcaccgaac tggccatagt atcacctacc
     2161 ctagccaggc accacctccc gcattcagcc tgtgatattt ccgaatattt acggcgattt
     2221 ggagccaagg ataatatgat cggcctgcga agaggagctg gcaagggcat atcccatata
     2281 tcagaagagg aaaaaaggct catagaggaa catgacttgg cggtattcgc cctgggaagt
     2341 ttccgggaat gtatactcaa aaaaactcat ttatttgagg atctggagat accgatagta
     2401 gtgactggag ccccagaaat tgatgtggaa ggtattcctg gtgcagatgc ctatgtgagt
     2461 ggcttgggaa ggatacctcg acgtttgaaa aggggagaaa atatacgtgc ccttaaaaaa
     2521 ctggcggagg tggtggaaaa aataatcgat aatcgtagaa ttgatatgag tgatgatcca
     2581 cctatcgtcc cgtctatact ggttaaaacg gagatagaaa accaggttcc agccattaag
     2641 aaggtttact ctccggcgcc cgtggttagt caactggatg gggtgcgggt taagctggat
     2701 tacgaccagt accacgagga aattgaaaac ttgatggttc aagattatcg tttaggagat
     2761 atagcagata taaaacgttc ttttatgtat aactacatat tggtcaagct tctatctgaa
     2821 acatccttgg tggaaccttt ggaacaggaa atatcccaga aagccacggc caagtgacag
     2881 gccaataaca acatactcct atcaggaatt taattgcaaa aatatcatct aaaaccctta
     2941 aaaatatcat gaaaaggatt ttacaagtag cgggttttgt ggcaatattt ttaagggttt
     3001 taga
//
LOCUS       DAOQVW010000155         3000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_38369_length_3000_cov_15.430560, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000155 DAOQVW010000000
VERSION     DAOQVW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38369_length_3000_cov_15.430560"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2130)
                     /locus_tag="PKK85_04250"
     CDS             complement(<1..2130)
                     /locus_tag="PKK85_04250"
                     /inference="COORDINATES: protein motif:HMM:NF021648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HNS25261.1"
                     /translation="MVKGNKELGIGVTGDISDITSKLDKLLDKIGLVKDKTVNLDVDV
                     LDEEQVTNLQNSMDKLTDKTVKMDLGGDAESKVKDLTDKVNKLGSETETVKVDADITP
                     AEGKINELRDQIEDLKGAAAGIVIGAGVTGSMEGAATRDEALAQIRAYMPESADEAER
                     LATEIFKATGADWGEVADGLVQVKTQTGLTGNELESVTEKSIRFSRMFDEDVREVVRS
                     ATQLSQTFGISMSDAFDIMTKTFQATGDPADDLLDTFNEYDQNFKDMGYSAEEFGNIL
                     ASGLKHGVMNTDQMADAIREAMIRLKTSPDEAKKVYDLIGASSEQQARWNKMLQAGGD
                     QAQQAFEEIVGSISQIEDPLKRQEANVALFGSKFEDQGDGINQAIMDNTDYLNGMGTS
                     FDTTSEKALSMGDTIKQALRSLPGGEWLSGILEGLWGFVTSDVGELITVGISGILGAI
                     GSKLLGGEGAKSALDWGKGIGGKILEGIQGLLPKSVSDLIGKIFKGGGKGAGVGMIFT
                     KEDLVGQEGSQTRTTWEEVFKGWGLSQEDLKKWNDSMNKGPLEDAKANTAALGQFITD
                     SASALSGVTTPITDTLNWLGESVEKWGSTAWDMITNFVGGLKGGLPDLDKTLDTLETK
                     IQDTLNWLIELPSKAWQWGWDIIDSWKRGFGESLDGAKGWIEDKLSYLSGLLEGHSPP
                     KEGPLSEIDVWGVNIGRSFVEGIGEGIG"
     gene            complement(2528..2953)
                     /locus_tag="PKK85_04255"
     CDS             complement(2528..2953)
                     /locus_tag="PKK85_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25262.1"
                     /translation="MVLLFKEPKTFQTPDKGEYVILPVQVDDMKILFRITNGREKLEQ
                     EATTALKKKLKKKTLTKEEKKEINISGEDFLDACGKDIKKLIDVTVINTKTGEPLPLK
                     YRQPGNVMELIGEIMDITNVDKKSSSEGDDTPLELKKTS"
BASE COUNT          701 a          783 c          552 g          964 t
ORIGIN      
        1 tccgatacct tcacctatac cctccacgaa gcttcggcct atattcacac cccacacatc
       61 aatctcactg agtgggcctt ctttgggtgg gctgtgaccc tccaataagc cggataggta
      121 gcttagttta tcctcaatcc aacccttagc cccatccaat gactccccaa accctctctt
      181 ccaactatct attatatccc atccccactg ccaagcctta ctcggtaact ctattaacca
      241 gttaagggtg tcctgaatct tagtttcaag ggtgtctaag gttttatcca agtctgggag
      301 gccccccttt aagcccccaa caaagttggt gatcatatcc cacgcggtag acccccattt
      361 ctccacgctt tcaccaagcc aattaagagt atccgtgata ggagtagtca ccccggataa
      421 tgcagatgca ctgtcagtga tgaattggcc taatgcagcg gtgttggcct tagcatcttc
      481 cagtgggccc ttattcatgg aatcattcca ttttttaaga tcctcttggc taagccccca
      541 tcccttgaac acttcctccc atgtggtacg ggtttggctg ccttcctgtc ccactaagtc
      601 ttccttggtg aagatcatcc ccactccagc tcctttaccc ccgcctttaa atatcttacc
      661 tatgagatca ctaacactct taggaagcaa accctggatt ccctctagga ttttccctcc
      721 aattccttta ccccaatcta aggctgattt ggctccttca cctccaagta atttggaacc
      781 aatagctccg agtatgccac taataccaac agtgattaac tccccaacgt cactagttac
      841 aaaaccccaa agcccttcta ggatcccact aagccattca ccccctggaa gacttctgag
      901 agcctgttta atagtgtccc ccatgctcag ggccttctcg gatgtggtgt caaagctagt
      961 tcccatacca ttaagataat cagtgttatc catgatagcc tgattaatac cgtccccctg
     1021 gtcctcaaac ttactcccaa acaatgccac attcgcttct tgacgtttca gtgggtcctc
     1081 aatctgacta atagacccca ctatctcctc aaaggcttgt tgtgcttggt cgccgccggc
     1141 ttggagcatc ttattccaac gagcctgctg ctcactagat gccccgataa ggtcataaac
     1201 cttcttagcc tcatccgggc tggtttttaa tcggatcatg gcctcacgga tagcatcagc
     1261 catctgatcc gtgttcatca caccatgctt aaggccagat gctaggatgt ttccgaactc
     1321 ctcggcactg taacccatgt ccttgaagtt ctgatcatac tcattaaaag tatccaagag
     1381 gtcatcagcc gggtcccccg ttgcttggaa agtcttagtc ataatatcaa aggcatcgct
     1441 catacttatc ccgaatgttt gggataattg agtggcggat ctgacaactt ctctgacatc
     1501 ttcatcaaac atcctactga aacggatact tttttcagtt acactttcca gttcattccc
     1561 agtcagcccg gtttgtgttt tgacttgtac taaaccatcc gctacttccc cccaatcagc
     1621 accagtggct ttgaaaatct ctgtggcgag tctttcagcc tcatccgctg attctggcat
     1681 atatgccctt atctgtgcaa gtgcctcgtc acgtgtggcg gcaccttcca tggacccggt
     1741 gacccctgct ccgatcacta tccccgcggc ggctcctttc aagtcttcta tctggtctcg
     1801 tagttcattg attttaccct cagcaggagt gatgtccgca tcaactttta ccgtttcggt
     1861 ttcgcttcca agcttattaa ccttatccgt taagtccttg actttggatt cagcatcacc
     1921 ccctaagtcc atcttcacgg ttttatccgt taatttatcc atactgttct gaaggttggt
     1981 aacttgttcc tcatctagga catccacatc taaattaacg gttttatctt tgactaatcc
     2041 tattttatcg aggagtttgt caagtttact ggttatatcg gatatgtcgc ctgtgactcc
     2101 gattccgagt tctttgttac ctttaaccat tttgtttatc tccaatacat gagggggggg
     2161 tttaaataag aataagaata taagatatac ccatgtcact cgcattaatt gtaatatctg
     2221 ctgctgtcgg cttcttgttt tatgaagtgt atacactcca gaaaaaggct aaaaaaatag
     2281 aagaatagct aagactgtga ttcttttttc ttagcttctg ctttccgtat aatatcctgc
     2341 tgaatactat tatacgtagc taatttattc atagcatcct cctcatcagg agtccccccg
     2401 aagaactgct ccgggttact ataatataat atataattcg caaactcagg gtaatagaac
     2461 cgccccactt catccgggga tactcccatt ttatgggtga ggtaggcttt cccagcgaat
     2521 atttgtttta tgatgtcttc ttcagttcta aaggggtatc atccccctca gatgatgatt
     2581 ttttatctac attggttatg tccattattt ctccgataag ttccatcacg ttacctggtt
     2641 gacgatattt gaggggtaat ggttcaccgg ttttagtatt aatgacggtg acatctatga
     2701 gttttttaat gtcttttccg caggcatcta ggaagtcttc gccgctgatg ttgatttctt
     2761 ttttttcctc tttggtgagg gttttctttt tcagcttctt ttttaaggcg gttgtggctt
     2821 cttgttctag tttttccctg ccgttggtga ttctgaatag gattttcatg tcgtctactt
     2881 gtacgggtag gatgacgtat tcccctttat cgggggtttg gaaggttttg ggttctttaa
     2941 atagtagtac catatttata ttcactcctc aacaatttag taaattaagt taaaaaaaaa
//
LOCUS       DAOQVW010000156         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_38955_length_2969_cov_3.148250, whole genome shotgun sequence.
ACCESSION   DAOQVW010000156 DAOQVW010000000
VERSION     DAOQVW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38955_length_2969_cov_3.148250"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PKK85_04260"
     CDS             <1..965
                     /locus_tag="PKK85_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS25263.1"
                     /translation="GGNFYLLIVLSALFYPVAAVLERILSDWVSGIFYALAASWVGVS
                     FFLLWGVIIHQILGQFFHVPPMMAGGLIISVTLLVSGYSIYNALQLKIKKLCLTIPGL
                     TREIKAVQLSDVHIGPIRNHSFLRNVVEQSNRLNPDVVFITGDLFDGSSQIDEKIIKE
                     LNRFRSPVFFTPGNHDYFQGLNEICNAISHTRIRLLRNQVYQFGELQVVGVDYSYQKG
                     YLKKTLDFLKIDKNKPTILLYHLPDGFDEARKAGVDLQLSGHTHNGQFFPFNFLVKLR
                     FPYLGGLYKNSGSCLHVSPGTGTWGPPMRWGSRCEVTSLLLKPI"
     gene            complement(1181..1771)
                     /locus_tag="PKK85_04265"
     CDS             complement(1181..1771)
                     /locus_tag="PKK85_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNS25264.1"
                     /translation="MSRPRRLRRILEEPQIRCFKPEGSNMESSESVKITIDEFEAIRL
                     RDYHNIHQKRSAEVMGISQPTFHRILSSARKKISKALIEGTPITIIGDDFITDKEKYM
                     CIDCGFKWLNPSKSHHYCPDCQSDDIELIKKDIHPEKADPTLILRKSYGGKGMGAGPP
                     LVCKCPNCGFESDKKRGVPCKNTTCPQCETPLCGIN"
     gene            1919..2221
                     /locus_tag="PKK85_04270"
     CDS             1919..2221
                     /locus_tag="PKK85_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25265.1"
                     /translation="MRYKCNKCEFEWYSPKKTYEKCPDCGSEDIYTISAENNPQRPVG
                     PIGMGRRRGYGGQGIGAGPPTKCKCTQCGYETEKVPGVPCRNSACPKCGAPLCGTD"
     gene            2235..2603
                     /locus_tag="PKK85_04275"
     CDS             2235..2603
                     /locus_tag="PKK85_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNS25266.1"
                     /translation="MISICIPVMENLGISSSISQHFGKTPYFALIKLENDEIKDLEVV
                     KSLGRHAGGTMTPAEAVSNTKAQVLVCGNLGSKAIQMLGEKGILVYVGASGTVETALK
                     QLQDGVLSLADDSAACSEGH"
     gene            2716..>2969
                     /locus_tag="PKK85_04280"
     CDS             2716..>2969
                     /locus_tag="PKK85_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNS25267.1"
                     /translation="MQSDEKDNEHKLAMMEEDIAITKNMAKIKHKIAVMSGKGGVGKS
                     TVSVNMALAFNQKGYHTGILDADLHGPNIPLMLGLEGRHL"
BASE COUNT          897 a          537 c          646 g          889 t
ORIGIN      
        1 gaggtgggaa tttttatctt ctgatcgtgt tatcagcact gttttatcct gtggccgcag
       61 tactggagcg cattttgtcg gactgggtca gtggcatatt ttatgctctg gctgcatcat
      121 gggtgggggt ttccttcttt ctactgtggg gggttataat acatcagata ctggggcaat
      181 tttttcatgt gccccccatg atggctggtg gattgataat tagtgtaacg cttcttgtca
      241 gcggatattc catctacaat gctttacaat taaaaatcaa aaaattgtgc ctgactatac
      301 caggtttaac ccgtgaaata aaggcagttc agttgagtga tgttcatata ggtcccatta
      361 gaaaccattc tttcctgagg aatgtggttg aacagtctaa cagactgaat cctgacgtgg
      421 tgttcataac cggcgaccta ttcgatggca gttcccagat agatgaaaag attatcaaag
      481 aacttaacag atttcgttcc ccagtatttt tcactcccgg aaatcatgat tactttcagg
      541 gcctaaatga gatttgtaac gccatatccc acactagaat ccggttattg cgtaatcagg
      601 tgtaccaatt cggtgaatta caggtggtgg gggtggatta ctcttaccaa aaaggatatc
      661 ttaaaaaaac actggatttt ctgaaaatcg acaaaaataa acccactata ctcctttatc
      721 atctgcccga tggttttgat gaggccagaa aggcgggggt tgatttacaa ctttctggac
      781 acactcataa tggccagttt tttccattca acttcctggt gaagttgagg tttccttatt
      841 taggaggttt atataaaaat tctggatcct gtttacatgt ttcccctgga actgggacat
      901 gggggcctcc tatgcggtgg ggatcccgtt gtgaggtgac ttcactcctt cttaagccta
      961 tttgatctgt agatttttat ggataagatc tctggacttt acttgctctt gactggattt
     1021 aaaattgtca ctgcaattat tagacacaga gttagcatgg ttaacattat tttatacatt
     1081 ttttcactct tttaacaact caattaggct tgaatgtcta aaaaaatttc cataaagaat
     1141 cttgaataca aacctcatgt tggaagataa aagctttaag ctaatttatc ccacacaggg
     1201 gggtttcaca ttggggacat gtcgtatttt tacagggcac gccacgtttt ttgtcagatt
     1261 caaatccaca gtttggacat ttacaaacta gggggggccc ggctcccatt cccttgccac
     1321 cataggattt tcttagaatc aaggttggat cagccttttc agggtggata tcttttttaa
     1381 taagctctat atcatcagat tggcagtcgg gacaataatg atggctttta cttggattta
     1441 accatttaaa tccacagtca atgcacatgt acttttcttt gtctgttatg aaatcatctc
     1501 cgatgatagt gatgggggtt ccctcaatta aagctttaga gatcttttta cgagcagagc
     1561 ttaatattcg gtgaaaggtg ggttgagaaa ttcccattac ctctgcagat cttttctgat
     1621 ggatattgtg gtagtcccgg agtcttattg cttcaaactc gtctattgtt attttcacag
     1681 attctgaaga ctccatgttg gaaccttctg gtttgaaaca tcggatttgt ggttcttcca
     1741 atatcctcct taaccttcta ggtctcgaca taagcgaata tatattcaaa aatctttata
     1801 aacattcggc aggtggaaaa aattcaaaca ctatatataa tagttatgaa tatatattca
     1861 taattgatcg gaatccaaat aacagaatta atggagtgga tcataaatag gtgataaaat
     1921 gagatataaa tgcaataaat gtgaatttga atggtacagc cctaaaaaaa cctatgaaaa
     1981 atgcccggac tgtggatctg aagatattta cactataagt gctgaaaaca acccacaaag
     2041 accagtagga cctataggga tgggtagaag aagaggttat ggtggtcagg ggataggggc
     2101 cggaccaccc actaaatgca agtgtaccca gtgtggatat gaaactgaaa aagtccctgg
     2161 agtcccctgt cggaattcag cgtgtccaaa gtgtggagca cctctatgtg gtacagactg
     2221 agggtgtggt aatcatgatc agtatatgca tccctgttat ggaaaatttg ggtatctcat
     2281 catccatatc tcaacatttc ggtaaaacac catacttcgc attgatcaaa ctagaaaatg
     2341 atgaaattaa agatctagaa gtagtaaaaa gtttaggaag gcatgctgga ggaacgatga
     2401 ctccagctga ggctgtgagc aacactaaag cccaagtatt agtatgtgga aatctgggct
     2461 ccaaagctat ccagatgctg ggagaaaaag ggattcttgt atatgttggg gcatcaggca
     2521 cagttgaaac tgcacttaaa cagttacagg atggcgtgtt aagtttagct gatgacagtg
     2581 ccgcttgtag cgaaggacac taatcaagaa atagatcaaa aaaaaattaa tttccatcag
     2641 atttagccaa gataatctta aggttcaatg gatgggaaag caagtatcat gaacactact
     2701 catagaagtg ggaaaatgca gtccgatgaa aaagataatg aacataaatt agcgatgatg
     2761 gaagaagaca ttgctattac caagaacatg gctaaaataa agcataaaat tgctgttatg
     2821 agtggaaagg ggggagtagg taaatcaaca gtttctgtga acatggcttt agcatttaat
     2881 cagaaaggtt atcataccgg tattttagat gccgatcttc acggccccaa catccccctc
     2941 atgttagggt tggaaggaag gcacctaaa
//
LOCUS       DAOQVW010000157         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_39250_length_2954_cov_3.533632, whole genome shotgun sequence.
ACCESSION   DAOQVW010000157 DAOQVW010000000
VERSION     DAOQVW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39250_length_2954_cov_3.533632"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="ahcY"
                     /locus_tag="PKK85_04285"
     CDS             complement(<1..1025)
                     /gene="ahcY"
                     /locus_tag="PKK85_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019268086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNS25268.1"
                     /translation="MSYDVKDMSLAPQGKKKISWVQKHMPVLEHIKKRFKKEKPFEGI
                     TIGSCLHLEPKTINLGLTLQAGGAEVAMTGCNPLSTQDDATAAGASLGLHMYGWREET
                     NEEYYQNLNRVLDHEPDIIIDDGADMIFLLHRNRRELLDNVIGACEETTTGIHRLRAM
                     HADGALLFPVMAVNDAYTKYLFDNRYGTGQSTFDSIMGSTNILIAGKTVVVCGYGWCG
                     RGIALRAHGLGANVIVTEIDPIRALEAKMDGYRVMSVREAVKHADILITATGNIDVVS
                     KDDFQVMKDGCILANSGHFNVEINRDDLKEMSKSNELLKPDIEEFQLPNGRKFYLLAE
                     GRLINLAG"
     gene            1429..1701
                     /locus_tag="PKK85_04290"
     CDS             1429..1701
                     /locus_tag="PKK85_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25269.1"
                     /translation="MRIVEESLIAFLLIVVFVVSISGCASNTWTSGPTDPDAAQKVGR
                     ADMWTSGVDKSQGFWNGEQDGENKKSTDTSEQNKTPEDSRTQENIK"
     gene            complement(1812..>2954)
                     /locus_tag="PKK85_04295"
     CDS             complement(1812..>2954)
                     /locus_tag="PKK85_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS25270.1"
                     /translation="DALDQALRRPGRFDREIEIGVPDKDGRREVLQIHTRGMPLDEKV
                     DLDEIAETTHGFVGADLESLCKEAAMRVLRRVLPDIKADEEIPKETLKKMIIKKADFK
                     EALKEIQPSALREVLVQVPNITWQDIGGLDNAKQELREAVEWPLKYPDSFEKFGVTPP
                     RGVLIYGPPGTGKTLLAKAVANESDANFIAVKGPELLSKWVGESEKGVREVFRKARQT
                     APTVIFFDEIDSIASTRGGGSTDSGVTQRVVNQLLTEIDGLEELQDVSVVAATNRVDI
                     MDPALLRPGRFDRHVKVDDPDHAARLEIFKVHTKNMPLAKDVDLERLATETDGYVGAD
                     IEAVCREAVMLTLRDNLDADKVTMMYFKKALKKVKTEEKPDLVQYH"
BASE COUNT          759 a          669 c          644 g          882 t
ORIGIN      
        1 ccggccaggt ttatcaacct cccctcggct agtaaataga atttacgtcc atttggtagc
       61 tgaaactctt caatatccgg ctttaaaagt tcattggatt tggacatctc ctttagatca
      121 tcgcggttga tttccacgtt gaaatgaccg gagttggcca ggatacatcc gtctttcatt
      181 acttggaagt catccttact cacaacatcg atgttgcccg tggcggttat gaggatgtca
      241 gcatgtttaa ccgcttcccg gacactcata acgcgataac catccatttt ggcttcaaga
      301 gcccttatgg ggtcaatttc agttacaatg acgttagcgc caagtccatg ggcccggagg
      361 gcaatacctc taccacacca tccatatcca cacacgacca cggttttccc tgcaattagg
      421 atgtttgtgg atcccattat ggaatcgaag gtggactggc cagtaccgta acggttatca
      481 aaaagatatt tagtgtaggc atcattcaca gccatcactg gaaatagtag ggcaccatcg
      541 gcatgcattg cccttagacg atggatgccg gttgttgttt cttcacaggc gcctatgaca
      601 ttgtctaaga gttctcttcg gttgcggtgt aggagaaaga tcatgtctgc accatcatcg
      661 atgatgatgt ctggttcatg atccagtact cgattaaggt tctggtaata ctcctcgttg
      721 gtttcctccc tccacccgta catgtggagt cccagggagg cgccggcagc agttgcatca
      781 tcctgagtgg agagtgggtt gcagccggtc atagccacct ccgcaccacc ggcctggaga
      841 gttaaaccta aatttatggt tttaggctcc aaatgcaggc aggaaccaat ggttatgcct
      901 tcaaatggtt tctccttctt gaaacgcttt tttatatgtt ccagaactgg catgtgcttt
      961 tgtacccaag atattttctt ctttccctgg ggagctaggg acatgtcctt tacatcgtaa
     1021 ctcatattaa cacctttttt taaaaaaatt agccgagata aaacttagga tgtctaaggc
     1081 taaagaaatt tacattcacc ataggttaac atcaaaaatt tgatcttaag tctgtttaaa
     1141 ctaggttctt caccaaatct ttttccaatt tactcaaaac atacatctag caaccttgaa
     1201 caacttgtaa ttaaatcata gctcagaaat ggctatcttt ttgaagtaaa cctgtgattc
     1261 aattatagat atatggtgat tctaataagt attttcaaat gatcgcttat tctatccgaa
     1321 gaatcatgga agataagtta attctttaca aacctacaaa tttgtgtttg gcattggata
     1381 attatatatt ccttgttgat aatactataa cttgaggggg gagtttcttt gagaattgtt
     1441 gaagaaagtt taatagcttt cttgttaatt gtagtttttg ttgtttcgat ttcgggatgt
     1501 gcatctaaca cttggacttc aggcccaaca gaccctgacg cagcacagaa ggtagggaga
     1561 gccgatatgt ggactagtgg agtggataag tcacaaggat tttggaatgg agaacaagat
     1621 ggtgagaata aaaaatctac agacacatcc gaacagaata agaccccgga agatagccga
     1681 acacaagaaa atataaagta gttcctgcca atgactcagt tgagtttaaa ttagacagga
     1741 aaataaggga aatttagtaa aaaaagtagt tataatgggg aattatgata gtattcccca
     1801 ttgatgggtg tttagtggta ttgcaccaga tctggttttt cttcggtttt aaccttttta
     1861 agggcctttt taaagtacat catagtcacc ttatcagcgt ctaggttgtc cctgagggtg
     1921 agcatcacag cttcccggca gacagcctcg atgtcggcac ctacgtaacc atctgtttcg
     1981 gtggccagtc tttccaggtc cacatccttg gccaggggca tgtttttggt gtgtacttta
     2041 aaaatttcta gccttgcggc atggtcgggg tcatccactt tcacgtgacg gtcaaacctg
     2101 ccgggtcgta gaagtgccgg atccatgata tccacccggt tggtagcagc caccacagac
     2161 acatcttgca gctcttcaag gccatcaatt tcagttaaaa gctgattaac aaccctttga
     2221 gttactccac tatcggtgct gccaccacca cgggtggaag caatggaatc tatctcatca
     2281 aagaagatta ctgtaggcgc agtttgcctg gcctttcgga atacttctct gacgcctttt
     2341 tctgattctc ccacccattt agatagaagt tctgggccct taactgctat gaagttagcg
     2401 tcgctttcat tggccacggc cttagccagg agcgtttttc cggttccagg tggaccgtag
     2461 atcagaacac cccggggtgg ggtgactccg aacttttcaa aactatccgg atacttaagg
     2521 ggccactcaa cagcttcccg tagttcttgt tttgcgttat caagccctcc aatatcctgc
     2581 caagttatat taggcacttg taccaggact tctctaagag cggatggttg tatctcttta
     2641 agggcttcct tgaagtcggc ctttttaatg atcatctttt taagggtctc tttgggtatc
     2701 tcttcatcgg ctttgatatc gggtaaaacc cggcgcagaa cccgcatggc agcttccttg
     2761 cagagagatt ctaggtctgc tcctacaaat ccgtgggtag tttctgcaat ttcatccaga
     2821 tccacctttt catccagggg cattcccctg gtgtggatct ggagaacttc tctcctccca
     2881 tccttatcag gtacaccaat ttcgatttcc cggtcaaatc tgcctggtcg tcgaagagcc
     2941 tggtccaggg catc
//
LOCUS       DAOQVW010000158         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_39300_length_2951_cov_4.019682, whole genome shotgun sequence.
ACCESSION   DAOQVW010000158 DAOQVW010000000
VERSION     DAOQVW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39300_length_2951_cov_4.019682"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1245
                     /locus_tag="PKK85_04300"
     CDS             22..1245
                     /locus_tag="PKK85_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNS25271.1"
                     /translation="MKATAEKIAEKVYWVGVLDWDIRQYHGYTLKGTTYNAYLVFGEE
                     KVALIDNAYHGNYQELMARVGDAFHREGKEVHIDVIIQNHVEMDHSGLLTELHRNFPE
                     APIYCTKIAVKGLLKHYPALKGANFVEVGTGETLELGGKTLTFLEAFLLHWPDSMFTF
                     LSEDGVLFPNDAFSQHLCFAQRYDNDVPEYILMEATQKFYANLLTPLSKLVLKKLQEV
                     TDLGLLEQIKIIAPAHGQIWTDPMKVIGAYNSWATGECEDKITIIYDTMHHSTQKMAH
                     AMAEGVISEGVDVKMYYLHEDERSEIVKEVLDSKAIALGVPTINDVPYPSAGDILYYL
                     RGLLFNRTGRERLAVTFGSMGGRGGSPHKLAEELTQYGFNVKEEYEITFIPDQDELEK
                     CFEVGKKLAQEIKTL"
     gene            complement(1545..2897)
                     /locus_tag="PKK85_04305"
     CDS             complement(1545..2897)
                     /locus_tag="PKK85_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS25272.1"
                     /translation="MKVIIIGGGAGGLSTASNIRKYDAKAKITVITRDKHVAYSPCAI
                     PYVLSGDVKNFNDIIMHQPEEYLDRNITVLTEAEVLEVEKEQNKIKYILMNDKKRHER
                     ELEYDRLVIATGGAPFIPPVKGANLKGVFTIHTIDDGFKIKKWANKSQTAVVVGAGLI
                     GLETAYGLKKMGLEVVVGELVSQIVPRLLDPDMARLVQNYLEKEGIRVILGNSIEQVM
                     GTHKVEGVQIGNEIIKADLVIMATGIRPETHLAEYIGCELGKWAIKVNEKMETSIPGI
                     YAVGDCVEVTDAITGHPTQSPLGSTAVRQAKIAAKNIAGQFAQAKPVLNATVAKIGEL
                     EFGAVGLTKTVAIYNGIFVISGKKRALTKARYYPGAKRIDVKMVCNLQGRIIGCQIVA
                     EERVAERVDTMALAIAKKVTCNELVNMEFSYAPPVSMVIDPIILAAENACEKLKEIGE
                     "
BASE COUNT          866 a          663 c          590 g          832 t
ORIGIN      
        1 tggtgaggag gtcgcaagat catgaaagca accgcagaga agatagcaga aaaagtttac
       61 tgggtaggag tcctggactg ggacatccga caatatcatg gctacactct aaaaggaacc
      121 acttacaatg catatctagt atttggagag gagaaagtag ccttgataga taacgcatac
      181 catggcaact atcaagagct catggccagg gtaggggacg cattccatag ggaaggtaaa
      241 gaggtccaca tcgatgtaat catacaaaac catgtcgaaa tggaccacag tggcctttta
      301 actgaattac accgtaattt cccagaagcc ccaatatact gcacaaaaat agcagttaaa
      361 ggactgttaa agcactatcc tgcactgaaa ggggctaact ttgtggaagt agggactggt
      421 gaaaccctgg aactgggtgg taagacctta accttcttag aagcctttct actgcactgg
      481 ccagacagta tgtttacatt cctatctgaa gatggagttt tattcccaaa cgatgctttc
      541 agtcaacacc tgtgctttgc acaacgttat gacaatgatg ttccagaata catcttgatg
      601 gaggccaccc agaagtttta cgctaatcta ctgacccccc tatctaaact ggttttgaag
      661 aagttacagg aagtcactga tcttggtctc cttgaacaga tcaaaatcat tgcaccagcc
      721 catggacaga tatggaccga tcctatgaaa gttattggtg cctacaattc ctgggcaact
      781 ggtgaatgtg aagataaaat taccatcatc tacgacacca tgcatcattc cacccaaaaa
      841 atggcccatg ctatggctga gggtgtaatc agtgaagggg tggatgttaa gatgtactat
      901 ctccacgaag atgaacgcag cgaaatcgtt aaagaggtcc tggacagtaa agccatagcc
      961 ctgggagtgc caaccattaa cgacgtacct tacccaagtg caggggacat tctatactac
     1021 ctccgaggat tgttattcaa ccgcactggc agggaaaggt tggccgttac attcggttcc
     1081 atgggtggaa ggggaggatc accccataaa ttggctgaag aacttactca atacggattc
     1141 aacgttaaag aagagtatga gataaccttc ataccagacc aagatgagct agaaaaatgc
     1201 ttcgaagtgg gtaaaaaact ggcccaggag ataaaaacac tataacaacg agattttggt
     1261 gcaacaagta aagaagttga attggaaaaa ctggtacaaa aagcttcaat ggatagtact
     1321 ggtaataaag ccccgaccca ttgggtttga atgaataaaa ggggtacttc taggagagac
     1381 ctatctctgt ccccatcata gccagggctg gaatcaaaaa aaatcaaacc ttgtaatgat
     1441 gaaaatttcc ccctatctgg ctctggcatg attataaatc aatcaagaat atagtacact
     1501 ttaaattact cattaataca ttgatgagat gaaaaacctt acccctactc tcctatttct
     1561 tttagtttct cacatgcgtt ttccgctgct aatattatgg gatcaataac catggataca
     1621 ggtggggcgt aggagaactc catgttaacc agttcattac aggttacctt ttttgctatg
     1681 gccagggcca tggtatccac tctttcagcc acccgctcct ctgccactat ctgacatcca
     1741 atgatccttc cctggaggtt acacaccatc ttaacatcta tccttttagc accaggatag
     1801 tacctggcct tggtaagggc cctctttttt ccagatataa cgaaaatacc attataaatg
     1861 gcgacagttt tggttaaacc aactgctcca aattctagct ccccaatttt ggccacggta
     1921 gcatttaaaa ctggtttagc ttgggcaaat tggccagcta tgtttttagc cgctattttc
     1981 gcctgtctta cggcagttga accaagtggg gattgggtgg gatggccggt gatggcgtct
     2041 gttacttcta cacagtctcc cacggcatat atgcctggaa tggatgtttc catcttttca
     2101 ttgactttga tggcccattt acccagttca caaccaatat actcagcaag atgagtttca
     2161 ggacggatcc cagtggccat tatcactaaa tcagccttta ttatctcatt ccctatctga
     2221 actccctcca ctttatgggt gcccattacc tgttcgatgg aatttcccag gataactctt
     2281 ataccctcct tttccaggta attctgaact aaacgggcca tatcggggtc cagtaatctg
     2341 ggaactattt gagaaaccaa ttcaccaaca actacctcca agcccatttt tttaagccca
     2401 taagcagttt ccaagccgat caaaccagct cccaccacaa ctgctgtttg actcttattt
     2461 gcccattttt tgatcttaaa tccatcgtca atggtgtgaa tagtgaaaac accctttaaa
     2521 ttagcacctt tcactggagg gatgaaggga gctccaccag tagcgataac caggcgatca
     2581 tattctagtt ctctttcatg gcgcttttta tcattcatta aaatgtactt tattttattt
     2641 tgctcctttt ctacctccaa tacttctgct tcagtcagca ctgttatatt ccgatcaaga
     2701 tattcttccg gctggtgcat tattatatca ttgaaatttt taacatcacc acttaaaaca
     2761 tagggaatag cgcatggaga ataggctacg tgtttatccc gagtaattac tgtaattttt
     2821 gcttttgcat cgtattttct aatatttgat gcggtggaaa gtccaccggc tccccctcca
     2881 attataatta ctttcattta tacaacctct gctggttatg aatatttttt ggaactaatt
     2941 cttagtaaaa g
//
LOCUS       DAOQVW010000159         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_39332_length_2950_cov_2.727461, whole genome shotgun sequence.
ACCESSION   DAOQVW010000159 DAOQVW010000000
VERSION     DAOQVW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39332_length_2950_cov_2.727461"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKK85_04310"
     CDS             <1..364
                     /locus_tag="PKK85_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25273.1"
                     /translation="NWQENYSVNFTQLRSRINEYISTIRVKAVPAFNQEDMVNLEVLS
                     ARIHSTSKKNGPVSIYDVAMEDPIILMAYTHLRNKGERRTVKNIRFELKALLDDEKIT
                     EHAYHTALDELKIVEDEK"
     gene            complement(385..636)
                     /locus_tag="PKK85_04315"
     CDS             complement(385..636)
                     /locus_tag="PKK85_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25274.1"
                     /translation="MGYLVCDKCRGYYKLEPDESPDDFEDECECGGRLEVVYNLANVF
                     EDINCPQCGYKNPGINLFCKECGAYLKMEGKSLMMDSGN"
     gene            complement(876..1100)
                     /locus_tag="PKK85_04320"
     CDS             complement(876..1100)
                     /locus_tag="PKK85_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04165 family Cys-rich peptide"
                     /protein_id="HNS25275.1"
                     /translation="MKAEELNKKCPECGCVNKSVSQKMTKEETKEHESDYVRHVPSGS
                     QGIIRCTDCGYIFEHCQDHPLPLEVKKKLI"
     gene            1397..2803
                     /locus_tag="PKK85_04325"
     CDS             1397..2803
                     /locus_tag="PKK85_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS25276.1"
                     /translation="MECKSKMVLWTVVSGMFLWSFGAGIVNISLPTIAQYLDISTSTV
                     SWVIIIHLLILVSLLLIFGRLADYLGQRNIFMWGVLVFTISSYLCGLSQEFNNLIFFR
                     LVQGVGSAMLLSVSPAMITLSSTIKNRGRAFGYISLATTLGLSTGYLAGGLVTEHLGW
                     NWVFFLNLPLGALVLIMSHLYLPDISNSASKDRFDFLGVCLSFTFFLSLILTLETLEI
                     NPYPLVGFLVTLMVGFFFIYWEFHHPHPLLDLRLFLNPHLIQSILSGFLTTLVLTGTI
                     FLVPFYLELVKSYPTDLAGLLVFASTLLVLVVGPLSGWLTDQVGAKLVNTTGALVLLG
                     SLVLLSLLDQTVGLLFIFTALAIRALSDGISNPANSKMVISHSPPGMESSVSSLLNTA
                     RYLGLVMGVVIFETIFNITISHHSPSLEASSQGALELTLPAAALVDGFHAAFYLGVGI
                     SVLIIIFTIMGRENREVL"
BASE COUNT          705 a          750 c          626 g          869 t
ORIGIN      
        1 taactggcag gagaactact ccgttaactt cacccaactc cgtagccgga ttaacgagta
       61 catctccacc atcagggtta aagctgtgcc tgcctttaac caggaggaca tggtgaacct
      121 ggaggtctta agtgccagga tccattccac atccaaaaaa aatggcccgg tatctatcta
      181 cgacgtagcc atggaggatc ccatcatact catggcctac actcatctta gaaataaagg
      241 tgaaaggaga actgttaaaa atataagatt cgaattgaag gccctcctgg acgatgagaa
      301 aatcacggaa cacgcctatc acaccgccct ggatgagcta aaaatagtag aggatgagaa
      361 atagaactct atacctggtt ttttctagtt tccactgtcc atcataaggc ttttcccttc
      421 catcttcagg taggcaccgc attccttgca gaacaagttg atacctggat tcttgtatcc
      481 gcactgtgga cagttgatgt cctcaaaaac gttggccaag ttgtacacta cttccagtct
      541 tcccccgcat tcacattcat cttcaaagtc atcaggtgat tcatctggtt ccaacttgta
      601 gtaacctctg catttatcgc acactaaata tcccattggt tatccccccc atttttcata
      661 actgatgggc atagcccacc acaggatatt gttgtaaaca aacaatgttg ttgtttatgc
      721 gtgtcattat atatatagat attcaatatc cttcaaccag tgaccctgga gatcccagtt
      781 atccctggcc aagaaaaagt aatagtctcc aaatttatat gtaaactcgg tatatccagg
      841 tttatcagtc ctgttttggc tttactgtgc ccggcctaga tgagcttctt ctttacttcc
      901 aggggtaatg gatgatcctg gcagtgctcg aagatgtacc cacaatcggt gcagcggatg
      961 atgccctggg agccagaggg tacatggcgc acgtagtcgc tttcatgttc cttggtttct
     1021 tccttggtca ttttttggga cacactttta tttacacagc cacattctgg gcatttttta
     1081 ttgagttctt cagccttcat ttttctagaa acccccatac tatgcaaata gtttggtatt
     1141 atacgaactg tatctaatag tacgaaccta ttctatttat agtttatcat ccccactatc
     1201 ctgaaaatat taaaagtcca tcctctagct gaagggccag ggaatgttcc tcccccctat
     1261 aggatttaat tatctaataa aatcattgca ccccatcccc ttccttcata gtatctactg
     1321 ttaattctag aaaaaaaatc agcccattat ttatggcaga gtatataact ctaaaaacac
     1381 tggaggccgg ataagagtgg agtgcaaaag taagatggta ctgtggaccg tggtgagcgg
     1441 catgttccta tggtcattcg gcgctggtat cgtcaacatc tccctaccca ccattgccca
     1501 gtacctggat atcagcacct ccactgtttc ctgggttata attatccacc ttttgatact
     1561 ggtatcctta ttactcatct tcggacgttt ggctgattac ctgggccaga gaaacatctt
     1621 catgtgggga gtgctggttt ttaccatctc ttcctatctg tgcggcctgt cccaggaatt
     1681 taataaccta atattcttcc gcctggtgca gggggtgggt tcggccatgc tactgtccgt
     1741 gtctccagcc atgataaccc taagttccac cattaaaaac cggggcaggg cctttggtta
     1801 catatccctg gccaccaccc tgggtctttc cactggatac ctggctggag gtctggtcac
     1861 tgagcaccta ggatggaact gggtgttttt tttaaactta cccctcggag ctctggtgct
     1921 tattatgtcc cacctctact taccagacat atcaaatagt gctagtaaag accggttcga
     1981 ctttttgggg gtctgtttat ctttcacatt tttcctgtcc ctgatattga ccctggaaac
     2041 cctggaaata aatccctacc ccttggtggg ttttttagta accctaatgg tgggattctt
     2101 cttcatctac tgggagttcc accaccccca tcccctcctg gatcttcgcc tgtttttaaa
     2161 tccccacctc atccagagca tattaagcgg ctttctaacc actctggtat taacggggac
     2221 catctttctg gtgcccttct acctggagtt ggttaaatcc taccctaccg atctagctgg
     2281 tcttctggta tttgcctcca ccctcctggt actggtggta ggacccctgt ctggttggct
     2341 cactgatcaa gtaggggcta agttggttaa taccaccggg gctctggtac tcctgggcag
     2401 tctagtccta ctgtccctgc tggaccagac tgtggggctg ttgttcatat ttacagccct
     2461 agcaatccgg gccctatcgg atggtatctc caatccggcc aacagtaaga tggttatcag
     2521 tcatagcccc ccagggatgg agagctcagt ctccagtttg ttaaacaccg cccgttatct
     2581 gggcctggtg atgggggtgg tgatctttga aaccatcttc aacatcacca tcagccacca
     2641 ctcccctagt ctggaggcat caagtcaggg tgccctggag ctaaccctcc cggcagcagc
     2701 actggtggat ggcttccatg cggccttcta cctgggagtg gggatatcag ttctcattat
     2761 catcttcacc atcatgggcc gggagaaccg ggaagtccta taagaatcgt aaatcgtaga
     2821 gatcatcagt ggagggtatg gtttccaggt atcccaggct ctgctgaagc ttcatgaacc
     2881 tctccatatt catggtatag tcaggatcgg ggtggtactg gaagttcaca tgttttaatg
     2941 cttctctttg
//
LOCUS       DAOQVW010000160         2946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_39396_length_2946_cov_3.241785, whole genome shotgun sequence.
ACCESSION   DAOQVW010000160 DAOQVW010000000
VERSION     DAOQVW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39396_length_2946_cov_3.241785"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="PKK85_04330"
     CDS             complement(<1..101)
                     /locus_tag="PKK85_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HNS25277.1"
                     /translation="MNLTTQKRLAADILKIGVNRVWIDPDHSEEVSQA"
     gene            complement(315..641)
                     /locus_tag="PKK85_04335"
     CDS             complement(315..641)
                     /locus_tag="PKK85_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HNS25278.1"
                     /translation="MNKPKFRRQEWFRYKKLGTKWRKARGKTSKTRRYEGRKPAMPTV
                     GYRTPRKTRGLHPSGYQDVLISNPKELEGLDPENQAARISSTIGDRKRELILARAKEL
                     GVKILN"
     gene            complement(646..1179)
                     /locus_tag="PKK85_04340"
     CDS             complement(646..1179)
                     /locus_tag="PKK85_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNS25279.1"
                     /translation="MALAAVIREEIPIPEGIEVTVDDTVTVKGSQGELSRKFKHHNVK
                     ISQEDGLVVLEARFPKKKDKAMLGTIKAHITNMMEGLTQGFTYRMKIVYAHFPMTVKL
                     AGGKVVIENFLGERYPRTAKVVGSAQVKIQGDEVTVSGVNKEDVGQTMANIEQATKIK
                     GKDPRVFQDGIYLVAKE"
     gene            complement(1189..1581)
                     /locus_tag="PKK85_04345"
     CDS             complement(1189..1581)
                     /locus_tag="PKK85_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNS25280.1"
                     /translation="MTLMDPLANALTNMRNNEMQGNNRCKIRPASKMIGHVLRTMQKE
                     GYIGEFEYVDDGKAGQFMVELEGNINKCGVIKPRHAVKKDEFEKFEKRYLPSKHFGIM
                     IMTTSQGIMTHHEAKEIGIGGRLLVYVY"
     gene            complement(1591..1743)
                     /locus_tag="PKK85_04350"
     CDS             complement(1591..1743)
                     /locus_tag="PKK85_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNS25281.1"
                     /translation="MKNMPRKYGKASRKCRRCGDHSALVRRYGLMLCRQCFRELAPKI
                     GFKKYN"
     gene            complement(1745..2251)
                     /locus_tag="PKK85_04355"
     CDS             complement(1745..2251)
                     /locus_tag="PKK85_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNS25282.1"
                     /translation="MNPMQKVRIAKATINIGVGEAGEKLARAEKLLETITHQKPVRTY
                     SKITNPEFGIRKGQPVACKVTLREEKAHDAVKMVLDGVGNKLRTSQFDRNGNVSFGIE
                     EHIDIPGMRYDPDIGIFGMNVSITFEKPGYRIKRRKIQRKQVSPKHQVTAEETQEYMR
                     EQFQVKIK"
     gene            complement(2252..>2946)
                     /locus_tag="PKK85_04360"
     CDS             complement(2252..>2946)
                     /locus_tag="PKK85_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HNS25283.1"
                     /translation="YKAPEHWPIHPKEDTWTVKPSPGSHAIEDSLPLLLVVRDILDLA
                     DTAREAKMIINQGEILVDGTVRKDYKFPVGFMDVIQIPKTGKTYRVLPDDKGRLVLHP
                     ISEENQNFKLCRIQNKTTIRGGKQQLNLHDGRNCLVEGEYRAGDVVVLQVPKQEVTQH
                     LKLENGALGFITGGKHIGELGTIKEINITKSSMPNTILMETGDGKSFQTLQDYVFVLG
                     KDQAMITLPGGK"
BASE COUNT          534 a          769 c          694 g          949 t
ORIGIN      
        1 gcctgggaca cctcttctga gtgatccggg tcgatccata ccctgttaac tcctattttt
       61 aggatatctg cagctagcct cttttgagtt gtaagattca taaagaaacc tccccttcaa
      121 ccccatttgg gggttgagtt aaaatttcta ctctccaccc cgaaaaatca ttccaggtgg
      181 atgggatgcg catctaatac tattgtcccg ggcatcccca tttttttcct ttcttgtagt
      241 atcacttatt gcttcgcctt tatttgtaaa cagaaccacc ccatgtgggg gggtgttatt
      301 ttacaaatgc ttccttagtt caggattttc actcccagtt ccttggccct ggcgagtatt
      361 aattcccttt tccggtcacc tatggtggaa ctgatacggg ctgcctggtt ctcagggtcc
      421 agtccctcca gttccttggg gttggatatc agtacatcct ggtatccgga tgggtgtagt
      481 ccgcgggttt ttctgggagt tcggtatcct accgttggca tggctggttt tctgccttcg
      541 tatcgacggg ttttactggt tttcccccgg gcttttctcc atttagttcc cagtttcttg
      601 tagcggaacc attcctgtct cctgaatttt ggtttgttca taggatcact ccttagccac
      661 caggtagatt ccatcctgga atacccgggg gtcttttccc ttgattttag tggcctgttc
      721 aatgttggcc atggtctgtc ccacgtcctc cttgtttact ccggagactg ttacctcgtc
      781 gccctgtatt tttacctggg cacttcccac caccttggcg gtgcgggggt agcgttcccc
      841 caggaagttt tcaataacta ccttgccgcc tgctagtttg acggtcatgg gaaagtgagc
      901 gtaaacaatt ttcatccggt atgtgaagcc ctgggtcagg ccctccatca tattagtaat
      961 atgggctttg atggttccca gcatggcttt atccttcttc ttggggaaac gggcctctaa
     1021 caccactaaa ccgtcttctt gactgatttt tacgttgtga tgtttgaatt tccgacttag
     1081 ctccccctgg gatcccttca cagtcaccgt gtcatccacg gtgacttcta tgccctcagg
     1141 gatgggtatt tcctccctga taaccgctgc taaagccatt gttatcacct aatatacata
     1201 taccagtaac ctgccgccta taccaatctc cttagcctcg tggtgggtca ttataccctg
     1261 ggaggtggtc atgatcatga taccgaagtg cttggatggc aggtaccttt tttcgaattt
     1321 ctcgaattca tcttttttaa cggcatgtcg gggctttata acgccacatt tgtttatatt
     1381 tccttctaat tcgaccatga actgtccggc tttgccgtcg tccacgtatt caaattcacc
     1441 gatgtatcct tccttttgca tggtccttaa cacatggcca atcatcttgg aggcaggtcg
     1501 gatcttgcac cgattgtttc cctgcatctc gttgttgcgc atgttggtaa gggcgtttgc
     1561 tagaggatcc ataagagtca taggtacacc tcaattgtac ttcttaaatc ctatttttgg
     1621 tgcgagttcc cggaagcact ggcggcagag cattaatccg tatctgcgta ccagggcaga
     1681 gtgatcccca catcttctac attttctgga tgcctttcca tatttccttg gcatgttttt
     1741 caccttactt aatcttcacc tggaattgct cccgcatgta ctcctgggtc tcctcggcgg
     1801 tgacctggtg cttgggggat acctgtttcc gttggatttt ccgtctctta atccggtaac
     1861 ccggtttttc aaaggttatt gaaacgttca ttccgaatat tccaatgtcc gggtcgtaac
     1921 gcatgccagg tatgtcaatg tgttcctcga ttccaaagga aacatttccg ttccggtcaa
     1981 attggctggt gcggagtttg ttacccaccc catccaggac catcttcacg gcatcatggg
     2041 ccttctcttc acggagggtg actttgcagg ccacgggttg acccttcctg atcccgaact
     2101 cggggttggt gatcttggaa taggttctca ccggcttctg gtgggtgatg gtttccagga
     2161 gcttctcagc ccgggccagt ttttctccgg cttctccaac accgatgttg atggtggcct
     2221 tggctattcg caccttctgc atggggttca tctacttacc ccctggtagt gtgatcattg
     2281 cctggtcctt acccagtacg aagacgtagt cctgcagggt ttggaagctt ttcccatcgc
     2341 ctgtttccat taggatcgtg tttggcatgg atgatttggt gatgttgatt tctttgatgg
     2401 ttcccaactc cccgatgtgc tttccaccgg tgataaaacc cagggcaccg ttttccagct
     2461 tcagatgctg ggttacttcc tgtttgggaa cctgcagtac caccacgtct ccggctcggt
     2521 attcgccctc gactagacag ttacgtccgt cgtggaggtt cagttgttgt ttccctcccc
     2581 tgatggtggt tttgttctgg atccggcaca gtttgaagtt ctggttctct tcactgatgg
     2641 ggtgtaagac cagcctcccc ttatcatcag ggaggacccg gtaggttttt ccggttttgg
     2701 ggatctggat tacgtccatg aatcccaccg ggaacttgta gtccttacgc accgtaccat
     2761 ccactaggat ctctccctgg ttgatgatca tcttggcttc ccgggcggtg tctgccaaat
     2821 ccagtatgtc ccggaccacc agtaggaggg gtagtgaatc ctctatggca tgggaacctg
     2881 ggcttggttt cacagtccag gtatcctctt tggggtgtat tggccagtgt tcaggtgctt
     2941 tgtatc
//
LOCUS       DAOQVW010000161         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_40254_length_2905_cov_3.095439, whole genome shotgun sequence.
ACCESSION   DAOQVW010000161 DAOQVW010000000
VERSION     DAOQVW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40254_length_2905_cov_3.095439"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1732)
                     /locus_tag="PKK85_04365"
     CDS             complement(110..1732)
                     /locus_tag="PKK85_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS25284.1"
                     /translation="MKISPLIQEAEDKLSYHRPWLKFYNKDTPSSLVYPDKTIYEMLV
                     DSAQQYPEHVALHFMGKKIKYKKLIQEVDNCARALLELGVTEKDSVTVCMPNTPHAVV
                     MFYAINQVGALANMIHPLSAPKEIEFYLEYVESKYILIGNFSYPNLKKIQGNLDLRKI
                     IVAKINDYLGKRKSMSFWAMEGHKIPKIRYNQDDLLISWKKFKKLGYQSSRTVESKKE
                     CGDGAVVLYTGGTTGVQKGVLLSNHNFNALVIQCLAHLNDERFGKISRDKIMCILPIF
                     HGFGLTICVHLPICVGACSILVPKFNANIFADQFAKYQPHYIAGVPTLYEALLRSKKM
                     KKSDFHGLKGVFAGGDTVPVELKRKFDGFMKKRGGQVTMQEGYGLTETVTVSCLVPHA
                     DDRLGSMGVPLPDMLYKIVKVGTTQDLAPGEEGEICISGPTVMLEYYNNPEETDKVLK
                     VHDDGFKWCHTGDLGYMDEDGFFYFRSRIKRMIKSSGYSVFPTQIEEVINRHEAVALS
                     CVIGVPDKYQIEKIKAFASRTHDRASRFYPSP"
     gene            complement(1742..2680)
                     /locus_tag="PKK85_04370"
     CDS             complement(1742..2680)
                     /locus_tag="PKK85_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNS25285.1"
                     /translation="MSKESREGSSYPRDKRRGDRGDGWILKDLSSFTKLIPHLMTTRN
                     SSAIYFKEKIDVTNFVGYVEEKNQELHQNPVKTLDGDVDKITYFHVFMAALVRLFTLK
                     PHLNRFIAGRNFYQRKKIEVAFVAKKEFTEEGEETIVKESFNRDETLWAVSSRLSKGI
                     KVAKTGQTDDATDILNTFARLPKPLVRLSLGFLDLLNYLGHYPDTIYQADPMHASVFV
                     TNLGSIGLKSVPYHHLYNRGTTSVFICLGEIHQDRLYDPGSGELVERYVVEISTTIDE
                     RISDGFYFIKAMNEFKEILNNPQLLEKKLEQFPVDI"
BASE COUNT          693 a          694 c          700 g          818 t
ORIGIN      
        1 cgttcgagcg ttggatggaa gcgcgcaggg tgcgcagcgc tgcaatcatg gaggtggatc
       61 atgccagcta aaacgaaaat cgaatgggct gattacatca gcaatccgat caaggcgaag
      121 gataaaaccg ggaagcgcgg tcatgcgtgc gtgaggcgaa ggccttgatc ttctcgatct
      181 ggtacttatc agggacacct atgacacagg agagggccac cgcttcatgg cggttgatga
      241 cctcctcgat ctgggtgggg aacaccgagt acccggaact tttgatcatg cgtttgatcc
      301 tggacctgaa gtagaaaaag ccatcctcat ccatgtaacc cagatcacca gtatggcacc
      361 atttaaatcc atcatcatgg accttcagga ccttatcagt ctcttcaggg ttgttgtagt
      421 actccagcat gacggtgggg ccgctgatgc agatctctcc ctcttcacca ggggcaaggt
      481 cctgggtggt gcccacctta acgatcttgt acagcatatc agggaggggc acacccatac
      541 tgcccagccg atcatcggcg tggggaacca ggcagctcac agtcacggtc tccgtcaatc
      601 cgtaaccctc ctgcatggtt acctggcccc cccttttctt catgaacccg tcgaatttcc
      661 ttttaagttc cacagggacc gtgtcacccc cggcaaaaac acccttcaag ccatggaaat
      721 cggatttttt catcttctta ctccggagca gggcctcgta gagggtgggt actccggcaa
      781 tgtagtgggg ctggtacttg gcaaactggt ctgcgaagat gttggcgttg aatttaggga
      841 ccagaataga acaggcccct acacagatgg gcaggtgcac acaaatggtg agtccaaatc
      901 cgtggaagat ggggaggatg cacattatct tatccctgct gatttttccg aatctttcat
      961 cgttcaggtg ggctaaacat tggatgacca gggcgttgaa gttatggttt gataataaaa
     1021 cccccttctg cacccctgtg gttccgcctg tgtataatac cacggcacca tctccacatt
     1081 cttttttact ctccacagtt cgactggact ggtaaccaag tttcttgaat ttcttccagg
     1141 agatgagcag gtcgtcctgg ttgtacctga tcttcggtat tttatggccc tccatggccc
     1201 agaagctcat gctcttcctt ttacccaggt agtcgttgat cttggccaca atgatcttcc
     1261 ggaggtccag atttccctgt atcttcttca ggttgggata ggagaagttg ccaattagaa
     1321 tgtacttact ctccacatac tccaggtaga actcaatctc tttaggggcg gagagggggt
     1381 ggatcatgtt ggccagggcc cctacctggt taatggcgta gaacatcacc accgcatggg
     1441 gagtgttggg catgcaaacc gttaccgagt ctttttctgt cacccccagt tctaagagtg
     1501 cccgggcaca gttatccact tcctggatga gtttcttgta ttttatcttc ttacccataa
     1561 agtgcagggc cacgtgctcc ggatactgct gggcagaatc cactaacatt tcatagatgg
     1621 ttttatcggg ataaaccaga ctggatggag tgtctttatt gtaaaacttt aaccagggcc
     1681 tgtggtagct taatttatcc tcggcctcct gtatgagggg tgaaattttc ataaaaatca
     1741 tctagatgtc tactgggaac tgttctaatt ttttttctag tagttggggg ttatttaata
     1801 tttccttaaa ttcattcatg gccttgataa agtagaatcc atcactgata cgttcatcta
     1861 tggtggtgga gatttcaacc acataccttt ccaccagttc ccctgatccc gggtcataga
     1921 gtctgtcctg gtgaatttct cccaggcaga tgaagaccga agtggttccc cggttgtaca
     1981 agtggtggta gggtacactt ttcagtccaa tactccccag gttggttaca aatacactgg
     2041 catgcatggg gtcggcttgg tagatggtat ccgggtagtg gcccaggtaa tttagaagat
     2101 ccagaaatcc cagtgagagt ctgaccagtg gcttgggaag cctggcaaag gtgttcagta
     2161 tatcggtagc atcatcggtt tggcctgttt tagccacctt aatcccctta ctcaatctgg
     2221 atgacactgc ccatagggtc tcatcccggt taaaagattc cttaacaatg gtttcttcac
     2281 cttcttctgt gaattccttt tttgctacaa aggccacctc gatcttcttt cgctgataaa
     2341 agttacgtcc agctatgaac cggtttaagt ggggtttgag ggtgaaaagc cggaccaggg
     2401 ctgccatgaa aacatggaag taagttatct tgtcaacatc tccatccagg gttttaacgg
     2461 gattctgatg taattcctgg tttttttcct ctacgtaccc tacgaagttg gttacatcaa
     2521 ttttttcctt aaagtatatg gctgaagaat tcctggtggt cattagatgg gggatgagtt
     2581 tggtgaagga actcagatcc ttcaaaatcc atccatcccc cctatccccc ctcctcttgt
     2641 cccggggata agatgatcct tctctacttt ccttggacat aattttctcc ctagactcct
     2701 attgattaaa gggatttaaa tattaacatc acaccagtat tagggctggt ttttaaaaaa
     2761 tttcattagc accaagattg cataatgtat aatatcaaga caatggtggg ggtaatagta
     2821 tgctggactt tggtatagct gaccaggcag agatggagaa ctacaccacc cgttccctga
     2881 aggaacttgc cccgggtgat gagat
//
LOCUS       DAOQVW010000162         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_40712_length_2883_cov_3.043494, whole genome shotgun sequence.
ACCESSION   DAOQVW010000162 DAOQVW010000000
VERSION     DAOQVW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40712_length_2883_cov_3.043494"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PKK85_04375"
     CDS             <1..886
                     /locus_tag="PKK85_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25286.1"
                     /translation="VYVVTVDNSGTITVTPASGGLAVLPPGQKGAIIHLRNTQGNPKY
                     GTAERVRQETAVMIGKMIRDGYPATDIMGEVFKEVSNDAGEKYGGGAVNLASSITTGE
                     MFTPPDINETGFPMNEPYSKVCPTCGWSVGYSGSENFQTCPLDGTELKTIYAYDALVK
                     SITSTGEGISVSVYGTDAAGISETTREIVQYSVKRNGFNAAKIAQDINSNINNGLLVG
                     VNYVEAKDINIRENSRAVGVYYKALPDKRTSPPWDLPLSSSILNIVGNMQTALGLILV
                     VLVLFRSTLLTSFTKKRR"
     gene            891..1265
                     /locus_tag="PKK85_04380"
     CDS             891..1265
                     /locus_tag="PKK85_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF356 domain-containing protein"
                     /protein_id="HNS25287.1"
                     /translation="MSLIMVRADSRDKILNSLADIERHAKLKIKGNPRIIQSKMADET
                     AKRIMNQNLKFKSSHSVLVLVESDTTQSIVQIRKIHPPAHLMVISEEYPEYAAMNSEF
                     QNLPHFKGYYSHKKRHNIEKKM"
     gene            complement(1334..1807)
                     /locus_tag="PKK85_04385"
     CDS             complement(1334..1807)
                     /locus_tag="PKK85_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HNS25288.1"
                     /translation="MRCEICGKKIIGQPTKTKIESSVMNTCKECSKFGKVQREPPKPQ
                     GQFRKTSPRPIRKFRSKKPSYEVIEDYNKVIRESREKRGWSREKLAEKIYEKVSVINR
                     IESGRMIPDMKLSRKLEKMLDIHILEKTDETIDEEVSSRDVREATIGDIARIKRN"
     gene            1949..>2883
                     /locus_tag="PKK85_04390"
     CDS             1949..>2883
                     /locus_tag="PKK85_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS25289.1"
                     /translation="MEKTSHNILKKIEDLKKEIKILKEENAKTKRNLMWKVRKLEKDK
                     VLIENEKMRLDRELKSLRGEIERFRSPPLVIATVTEVLDEGKVVVKSSTGPHFVIGYS
                     RFMDDKDLEPGARVALNQQTFSIVNVLPSEKDPIVTGMEVEEKPEVSYAQIGGLEEQI
                     VEIKETVELPLKKPELFKEIGIEPPKGVLLYGPPGTGKTLLAKAVAHETNATFIKIVA
                     SEFVRKYIGEGARLVRGVFELAKEKSPSIIFIDEIDAIAAKRLKSSTSGDREVQRTLM
                     QLLAEMDGFEARGDVGIVAATNRPDILDPGYRVDW"
BASE COUNT          904 a          570 c          591 g          818 t
ORIGIN      
        1 tgtttatgtt gtaacagtgg ataacagtgg aaccataacc gtaacccccg catcaggagg
       61 acttgcggta ctgccacccg gacagaaagg tgctatcatc cacctcagaa acacccaagg
      121 aaaccctaaa tatggaacag ccgaaagagt tcgccaggaa actgcagtta tgattggtaa
      181 aatgattcga gatggatatc cagctacaga cattatgggc gaggttttca aggaggtttc
      241 caatgatgct ggagaaaagt atggtggtgg tgcagttaac ttagcttctt ccattaccac
      301 cggggagatg ttcactcccc ctgatattaa cgaaactggt tttcccatga acgaacctta
      361 cagtaaagtt tgtcccactt gtggatggag tgtcggctat tccgggtcag agaactttca
      421 aacttgtcct ctagacggaa cagagcttaa aaccatctac gcctacgatg ccctggttaa
      481 atctattaca tctactggtg aaggtatcag tgtttcggtt tacgggaccg atgctgcagg
      541 tatcagtgaa accacccggg aaattgtgca gtactcggtg aaacgtaatg gttttaacgc
      601 agcgaagatc gcccaagata tcaactccaa tattaacaac gggcttttag tgggagtaaa
      661 ttacgtcgaa gccaaggata tcaacattcg agagaattcc cgtgccgttg gagtttacta
      721 taaagcattg cctgataaaa ggacttcacc accctgggat ctgcccctta gttcttccat
      781 actaaacatt gtaggaaata tgcagaccgc cctgggattg attctagtag ttctggtgtt
      841 attcagaagc acccttctaa cctctttcac gaagaagaga cggtgatgaa atgtccttaa
      901 ttatggttag agcagatagt agagataaaa ttctaaacag cctcgcagat atagaaaggc
      961 acgctaaact taaaataaaa ggcaatccac gaattattca atcaaagatg gctgatgaaa
     1021 ctgcaaaaag aataatgaat cagaatttaa agtttaaatc ttctcattcc gttttggtac
     1081 tggtggaaag tgatactacc caaagtattg tccagataag gaaaatacat ccccccgccc
     1141 acttaatggt cattagtgaa gaatatcccg aatatgctgc tatgaattct gaatttcaaa
     1201 atttacccca ttttaagggc tattattccc ataagaagcg gcataatata gaaaagaaga
     1261 tgtaattggt agatctaaat ttttttccat aattcttcca attaactatt cataatttaa
     1321 caacaagcac gtattaattt ctttttatac gggcgatgtc tccaatagtt gcttccctta
     1381 catctctgga actaacttcc tcgtcgatgg tttcgtcagt cttttccaga atatggatat
     1441 ccagcatttt ttctagtttt ctggaaagtt tcatatccgg gatcatacgg ccggattcaa
     1501 tgcggttaat tacggatact ttctcataaa tcttttccgc aagtttttca cgactccatc
     1561 cccttttttc cctggattct cttataacct tgttataatc ctcaatcact tcatatgatg
     1621 gtttttttga cctgaatttt ctaataggcc tgggtgaagt ttttctaaat tgtccctgtg
     1681 gttttggagg ttctctttgg actttaccga attttgaaca ttctttacaa gtgttcatta
     1741 ccgaactttc gattttggtc tttgttggtt gtccaattat tttttttccg caaatctcac
     1801 atctcatggc gatcccttta atcttaactt aaatttataa ttctacacct caatatttaa
     1861 atattattaa agaccataga aagcatcata taataaagtg ttagaaatta ttttttttcc
     1921 agaaatcaat gaggagtgaa catttaagat ggaaaaaaca tcacacaaca ttctgaagaa
     1981 aattgaagac ctgaaaaaag agattaaaat cctcaaagag gaaaatgcca aaaccaaacg
     2041 taacctcatg tggaaggtta ggaaattaga aaaagacaag gttctgattg aaaacgaaaa
     2101 aatgaggctg gaccgtgaac tgaaatctct ccgcggagaa attgaaaggt tccgatctcc
     2161 gccactagta attgccactg taactgaagt actagatgag ggtaaagtag tggtaaaaag
     2221 cagcaccggc ccccattttg taattggata ttcacgtttc atggatgata aggacttaga
     2281 acctggagcc agagtggccc ttaatcagca gactttcagc atagtaaacg tcttaccatc
     2341 agaaaaagac cccatagtga ctggtatgga ggttgaagaa aaaccagagg tgtcttacgc
     2401 tcaaattggc ggattagaag agcagattgt tgagataaaa gaaacagtgg aactgccact
     2461 taaaaaacca gagttattta aggagatagg tatagaacct cccaaaggag tgcttctata
     2521 cgggcctccc ggaaccggaa aaactttatt ggccaaagcc gtagcccatg aaaccaatgc
     2581 aaccttcata aagatcgtgg cctctgaatt cgtacgcaaa tacattggtg aaggtgcccg
     2641 tctagtgaga ggagtattcg aactggctaa ggaaaaatct cccagcataa tatttataga
     2701 tgaaattgat gctatagctg ctaaaaggct taaaagttcc actagtggtg atcgtgaagt
     2761 tcaacgtacc cttatgcaac ttttagcaga gatggatgga ttcgaagcac gaggtgatgt
     2821 gggtatagtg gccgctacca atcgacccga tatcctggat ccaggatatc gggtcgattg
     2881 gta
//
LOCUS       DAOQVW010000163         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_40764_length_2880_cov_3.717876, whole genome shotgun sequence.
ACCESSION   DAOQVW010000163 DAOQVW010000000
VERSION     DAOQVW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40764_length_2880_cov_3.717876"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PKK85_04395"
     CDS             <1..1031
                     /locus_tag="PKK85_04395"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNS25290.1"
                     /translation="DPYEVYTGRGPFEAAGVGVEVRSGDIAFRCNFSTANEDGIITDR
                     RAGRIRKGAKEIAQKINGMHLEYGVEVIFKESTGHRAVLVLRGAGLSDQVSDADPKYE
                     GNPPKEVVALDDSEEAHKTARILNELVEASYQVLKDHPVNQERVKNGENPANIILPRG
                     AGAVPEVQSFQEKYGLKAACIAETGLILGIGKITGMDLIVPEGATGGVDTDLDSIAKC
                     IMDTAAEDYDFILINIDGADESGHDGNLKDKIDFLRRVDSVIGQVMQLEDVIFILTAD
                     HSTPISIMDHAGDPVPLVISGSPVRVDDVEEFNERAAAKGGLCRIRGSDIMNIIMDLT
                     NRATKFGA"
     gene            1036..2397
                     /gene="glmM"
                     /locus_tag="PKK85_04400"
     CDS             1036..2397
                     /gene="glmM"
                     /locus_tag="PKK85_04400"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNS25291.1"
                     /translation="MEEIPQLFGTSGIRGRLGEKINPELALKVGLAVGTVAGEGKIVV
                     GHDPRTSSPMLSLAVKAGILQCGKDVLDLGMVPTPVVGYATQYLGAQAGVMITASHNP
                     SPYNGIKLWNPNGMAYKQEQERVIERIIHHESFKRVSWDLIGKSKDINNFISIYVENL
                     LDLVKIKPGLKVVVDCGSGAASYLSPLIIRKSGCEVLTLNSQPDGFFPGRDPEPSQKN
                     LGELMRVVKATGADLGLAHDGDADRMVAVDDKGRMADFDKLLALVSAQRGGTVVTTVD
                     ASAAIDHSLKEVQGKVDRTKVGDVHVATRISQLKANFGGEPSGTWIHPEFCMCPDGIL
                     SGLRVIELVQENGPLSQLLDELPTYPTRRVTVNCQDEDKKSIMQEMEKKLPQLFSDIK
                     DVNPIDGVRLTLNDDSWVLVRPSGTESFIRITLEGKNEKRAREIHEKSKKLLEGTIKS
                     FEG"
     gene            2400..>2880
                     /locus_tag="PKK85_04405"
     CDS             2400..>2880
                     /locus_tag="PKK85_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNS25292.1"
                     /translation="MRGIILTAGEGTRMRPLTLTRPKTMLPVGGKPLLQYNLEALRDA
                     GVKNITMVVGYYQEVVEEHFQDGVKFGVQIEYVTQEKRLGTAHAIATAASSVEEEVII
                     LNGDILVQSQVIKSLIEKYHRTNCDTILTLTEVDNPSSFGVVELQNDKITDIIEKPAP
                     "
BASE COUNT          841 a          597 c          760 g          682 t
ORIGIN      
        1 atgatcctta tgaggtttac actggacgtg gcccttttga agctgcagga gtaggagttg
       61 aggtaagatc aggagacatt gcattccgtt gcaacttttc cactgcaaat gaggatggaa
      121 ttattacaga ccgccgagca ggaaggatca ggaagggtgc caaggaaatc gcccagaaaa
      181 ttaatggcat gcacttagaa tatggggtgg aagtgatctt caaggagtcc accggacacc
      241 gtgcggtgct ggttctgcgg ggggccgggt tgtctgatca agtctccgat gccgacccaa
      301 aatacgaagg aaacccaccg aaagaagtgg tggcccttga tgactctgaa gaagcccata
      361 aaactgcacg catactaaat gaactggtgg aggcatccta ccaagttctg aaggaccatc
      421 ctgtgaatca ggaaagggtc aaaaacggag agaacccagc caacatcata ctacctcgtg
      481 gtgcaggggc agtccctgag gtgcagtctt tccaggaaaa atatgggctt aaagcagctt
      541 gcattgccga aactggactc attctaggta taggaaaaat tacaggaatg gatctaattg
      601 tgccagaagg tgctacaggt ggagtagaca ctgatttgga cagcatcgcc aagtgcatta
      661 tggacactgc agctgaggat tatgatttta ttctgattaa cattgacgga gccgatgaat
      721 ccggtcacga cggtaactta aaagataaaa tagactttct cagaagggtg gacagcgtta
      781 tcggccaggt catgcaactg gaggatgtga tttttatcct caccgcggat cactccactc
      841 ccatctccat tatggatcat gccggtgacc ccgtgcccct ggtaatcagt ggttcaccag
      901 taagggtgga tgatgtggaa gaattcaatg aaagggcagc ggccaaggga ggtctgtgcc
      961 gaatcagggg aagtgatata atgaacataa ttatggactt aaccaaccga gcaactaaat
     1021 ttggagcttg atttaatgga agagattcct cagctttttg ggacttctgg gattcgaggt
     1081 aggttgggtg aaaaaataaa cccggaatta gccctaaaag tgggcttggc cgtgggtacc
     1141 gtcgcaggag agggtaaaat agtagtgggg catgaccccc gtacatccag tcctatgcta
     1201 tccctggctg tgaaggctgg tattctccaa tgtggtaaag atgtgttgga tttggggatg
     1261 gttccaaccc cagtggtggg ttacgccacc caatatttag gagcccaggc cggggtgatg
     1321 atcaccgcct cccacaatcc atccccctat aatgggatca aactctggaa ccccaatgga
     1381 atggcttaca agcaggaaca ggagcgggtc attgaacgaa taatacatca tgaatcattt
     1441 aaaagggttt catgggactt gataggcaaa tcaaaggata taaacaattt tatttccatt
     1501 tatgtggaga atttactgga tcttgtgaaa attaagccgg gtctgaaggt tgtggttgac
     1561 tgtggaagtg gtgcagcctc ttatctgtca ccccttatta ttcgaaagtc tggatgtgag
     1621 gtattaaccc ttaattctca acccgatggt ttcttcccag gaagagatcc cgaaccttcc
     1681 cagaagaatc taggggaact gatgcgagtg gttaaagcca ccggtgcaga tctgggccta
     1741 gctcatgatg gagacgcaga tcggatggtg gccgtggatg ataagggtag gatggcagat
     1801 tttgacaagc tcctggccct ggtcagtgcc cagagaggag gtacagtagt aaccaccgtg
     1861 gatgcttcag ccgccattga ccactccttg aaagaggttc agggtaaggt ggaccggacc
     1921 aaggtagggg atgtgcacgt ggccactagg atctcccagc taaaagccaa ctttggaggt
     1981 gaaccatctg gtacttggat ccatcccgag ttttgcatgt gtcctgatgg gatactctcg
     2041 ggattgcgag taatcgaact ggtacaggaa aatgggccat tatcccaact tttagatgaa
     2101 ttacccacct atcccactcg cagagtcact gttaactgcc aggatgagga taaaaaatcc
     2161 atcatgcaag agatggaaaa gaaactgcct caacttttca gtgatatcaa agatgttaac
     2221 ccgatagatg gagtccgatt gacccttaat gatgatagtt gggttttagt aagaccttcc
     2281 ggaaccgaat cttttattag aatcaccttg gaaggtaaaa atgagaaaag agcccgtgaa
     2341 attcatgaaa aatcaaaaaa acttctggaa ggtactataa aaagttttga gggataaaaa
     2401 tgaggggaat aatactcact gctggagaag gaaccagaat gcgaccttta accctcaccc
     2461 gacccaagac catgctaccg gttgggggta aaccccttct tcagtacaac ctagaggcac
     2521 tacgggatgc tggggtgaag aatataacca tggtagtggg ctactatcag gaagtggtgg
     2581 aggaacattt ccaggatggg gtgaagtttg gggtgcaaat agagtacgta acccaggaaa
     2641 aacgcttggg aactgcccat gccatagcca ctgcggcttc cagtgtagaa gaagaagtta
     2701 ttatcttaaa cggtgacata ttagtccagt cacaggtaat taaaagtctc attgaaaagt
     2761 accatcgcac caactgtgac accatcctaa ctttaaccga agtggacaac ccatcttctt
     2821 ttggggtggt ggagcttcaa aatgataaga taactgatat catagaaaaa ccagcccctg
//
LOCUS       DAOQVW010000164         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_40768_length_2880_cov_3.175929, whole genome shotgun sequence.
ACCESSION   DAOQVW010000164 DAOQVW010000000
VERSION     DAOQVW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40768_length_2880_cov_3.175929"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..945)
                     /locus_tag="PKK85_04410"
     CDS             complement(286..945)
                     /locus_tag="PKK85_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HNS25293.1"
                     /translation="METVKVLVYNGDGTLSKSVEGVLECLNTINHQNLTPYYYYDYST
                     TNVVSTSKLSDCDVLIVPGGDASTYLRGRSIDAAAIKQFVSGGKGYMGICAGAYAATN
                     QFDRYRSGWGLASTINTNNVIYQGMVPVNISSNGDILGNGTSMFNLHHQNGPAMYSTS
                     SDIPMATYANNQTGYQGYAAIFGVNYGSGRVLLSGPHPEMDPQNPRLLARMILWTAKK
                     I"
     gene            1498..>2880
                     /locus_tag="PKK85_04415"
     CDS             1498..>2880
                     /locus_tag="PKK85_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS25294.1"
                     /translation="MSQKILLDREKIQNSSTYELLNILSSTRGGLTSKEALRRQEIYG
                     PNQIMEEKVSPIFTFLKYFWGPIPWMIEIAIVISAFIGHYADLGIITVLLFLNGVVGF
                     WQEYKANNAIELLKEKLAVNARVLRDGKWMEMAAQDLVPGDIVHLRLGNVVPADLKLI
                     KGDFLSIDESALTGESLPVEKKSSDVAYSGSVIIQGEMEALVILTGMNTYFGKTAQLV
                     EEAETKSHLKKAVVKIGDYLILVAAMLVAMIFMVALFRKESLLSSLQFALVLVVASIP
                     VALPAVLSVTMAIGAIALAKKEAIVSKLSSIEEMAGVDVLCSDKTGTITKNELAVAGL
                     EPFPGFDEKQLLLYAALASEEESGDPIDGAILKKTRTEIGPVGDEYNIKEFKPFNPVV
                     KRTEALVEGGGKRFGVSKGAVQSIQSLALEDPAVQVEETVDKFAKKGYRSLGVGKRDS
                     GGGWSYVGIIA"
BASE COUNT          791 a          589 c          667 g          833 t
ORIGIN      
        1 gatgaaggac cccctctctc agtggacatt gaccaggaac tccccctact cagggtgatc
       61 ctggacttcc agaagaagta atggattcaa ggtgacatca ttactttggg gacttctttc
      121 tcggcatcca acctttttgg ggtaggtcac gctttagatg ttttcccact tttttctcca
      181 cctatcacac cctaatggcc tacttgattt aagggttgca gatgtgtttt tgtggataga
      241 atgttggatg tgtggcttga acccctgtaa tcaatgaaat acttatcata tctttttagc
      301 agtccacagg atcatgcggg ccagtaaccg ggggttttga ggatccattt caggatgggg
      361 accacttaac agcacccttc ccgaaccata gttcacaccg aaaatggcag cgtaaccctg
      421 atatccagtc tggttattgg cataggtagc catggggata tccgaggacg tggagtacat
      481 ggcagggccg ttttgatggt ggaggttaaa catagacgtc ccgtttccca agatatctcc
      541 attggagctt atgtttactg gtaccattcc ctggtagata acattattag tattaatggt
      601 ggatgccagg ccccaacctg aacggtatct gtcaaactgg ttggtggcag cgtaggcacc
      661 agcacatatc cccatatatc ctttaccgcc actgacgaac tgtttaatag ccgcagcatc
      721 aatgcttctt cctcgaagat aagtggaagc atcacctcct ggaactatca acacatcgca
      781 gtcagataac ttgctggtgc tgaccacatt ggtggtggaa taatcgtagt aataatatgg
      841 ggtgagattc tgatggttta tggtgttaag acattccagg acaccttcca cgcttttgga
      901 cagggtacca tccccattgt acaccagaac tttaactgtc tccacctctt tcagggtatc
      961 actagtattg gtatccgcgt taaggggata tcccagtatg attaaagagg aaaatacaaa
     1021 gtagatgatg gcacctatga ttaataaaat tttaaattta tttatggttt aacctcctcg
     1081 gtctaaactt aaatgttaac tccagtccat tataaaggta gtttttccct gattgaccct
     1141 tattcccatc aacctagact acattcattt cctaatcata ggtgatggga caggaaaaaa
     1201 gcaactgcct atctgagtaa ttcctgtgaa acttgttcct acatcttttg aatatcagag
     1261 cccagcattg ctcctttttt ttcacaatcc cattgttact gaaattatct ccagcactgg
     1321 ttagtcaaat tcattgattc atcctagttc attagctaat aatcaataca agctattatt
     1381 ttcaatataa tcagtctaat tgcatatttt atatattttg ttgaacaaaa ttatttgtac
     1441 aataaataat atctttgggt gtccattaac catgcgcccc attgaacatg cctccttatg
     1501 agtcaaaaaa ttttattaga tagagaaaag atccagaata gttccacata tgaattatta
     1561 aatattttat cctcaactag gggcgggctt acttcaaaag aggcgttgag gcgtcaggaa
     1621 atatatgggc ccaaccagat tatggaagag aaagtcagcc ccatctttac ctttttaaag
     1681 tacttttggg gtcctattcc ctggatgatc gagattgcta ttgtgatatc agcattcata
     1741 ggacactacg ctgatctggg gataattact gtactgttat ttcttaatgg ggttgttggc
     1801 ttttggcagg aatataaagc caacaatgcc attgaactct taaaggagaa gttggctgtt
     1861 aatgcccggg ttctgcgtga tgggaagtgg atggaaatgg cagcccagga tcttgttcct
     1921 ggagatattg tacatctacg tctgggaaat gttgttcctg ctgatttgaa gttgattaag
     1981 ggagattttt tatccattga tgaatcagcc ctcactggtg agtccctgcc cgtggaaaag
     2041 aaatcttccg atgtggccta ctcaggctcg gtgataatac aaggagaaat ggaggcactg
     2101 gtgatcctca ccggtatgaa cacctacttt ggtaaaaccg cccagttggt agaggaagct
     2161 gaaacaaaaa gccacctaaa gaaggccgtg gtgaagatcg gggattatct gatacttgta
     2221 gctgccatgc tggtggcaat gatattcatg gtggcgttgt ttcggaagga gagcttacta
     2281 tcctccctcc aatttgcact ggtactggtg gttgcctcca tacccgtggc actgcctgct
     2341 gtgttatcag taaccatggc cataggtgcc attgccctgg ccaaaaagga ggccatagtc
     2401 agtaagttaa gttccattga agaaatggct ggtgttgatg tcttatgttc agataaaacc
     2461 ggtacaataa ccaaaaatga actggcagtg gcaggattag aaccctttcc agggtttgat
     2521 gaaaagcagc tgcttttata tgcagcactg gcctctgagg aggagagtgg agatcccatt
     2581 gatggcgcca tactcaagaa aaccaggaca gaaataggcc cagtaggtga tgaatacaat
     2641 ataaaagaat ttaaaccctt taatcctgtt gtgaaacgta ccgaagcctt ggtagagggt
     2701 ggtgggaaaa ggttcggggt ttccaagggg gcggttcagt caatacagtc actggccctg
     2761 gaagaccctg ctgttcaagt ggaagaaacg gttgataaat ttgcaaagaa gggctatcgg
     2821 tccctgggcg ttgggaaacg tgactcaggg ggtggatgga gttatgtggg tataattgcc
//
LOCUS       DAOQVW010000165         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_40793_length_2878_cov_8.521077, whole genome shotgun sequence.
ACCESSION   DAOQVW010000165 DAOQVW010000000
VERSION     DAOQVW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40793_length_2878_cov_8.521077"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..364
                     /locus_tag="PKK85_04420"
     CDS             125..364
                     /locus_tag="PKK85_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25295.1"
                     /translation="MKRERPVLRMYASDKKELLDPKSLEGFKKGDPIALIYGPDLISL
                     IDDLKELLEDYLILYERYEDCQAVIKKHIQKQGGG"
     gene            372..626
                     /locus_tag="PKK85_04425"
     CDS             372..626
                     /locus_tag="PKK85_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25296.1"
                     /translation="MNNDDILETEHQERIRDIHQKIDNIEYNIKRSFDLMILYLHLTN
                     REDCSPEFRRAINDFIGKYLEKSELSIPEILEREYPEHAE"
     gene            complement(939..1244)
                     /locus_tag="PKK85_04430"
     CDS             complement(939..1244)
                     /locus_tag="PKK85_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25297.1"
                     /translation="MNKSKMKQTRPGHYQKKGFTLHDFPTRREYLEHNLKVANRQLQH
                     IKQRLQVVEPLTRRDVVPLRGPSYDLQLDSLITKRRDLEGWVKSMEHELNTLEEKEK"
     gene            complement(1273..1818)
                     /locus_tag="PKK85_04435"
     CDS             complement(1273..1818)
                     /locus_tag="PKK85_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25298.1"
                     /translation="MDKIKLPEPNPEILERISDLRTEIYELKAKATRDGPNKIGGYKD
                     GLNILKREAEHLREKAGLMPELFDKDYKAKLKEIEKVEKLLVAAEKETQEIRDKYHEL
                     NGSTLQNLEGEVYIPYVIELNRLFWDLQARAFQENNPEYDKKLFEIAQLLDNPPALKY
                     MRVARRTKRNPGRVLVPNAIK"
     gene            complement(1850..2080)
                     /locus_tag="PKK85_04440"
     CDS             complement(1850..2080)
                     /locus_tag="PKK85_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25299.1"
                     /translation="MLHRVKRRPRARKRTRADKARQTLNKSLQAREALILELNQTENL
                     KEYDFLIGLLHEVEAVEGKAKKKIKETLRGNK"
     gene            complement(2087..2647)
                     /locus_tag="PKK85_04445"
     CDS             complement(2087..2647)
                     /locus_tag="PKK85_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25300.1"
                     /translation="MNPKIGKLRREYDDLVEDISPEQMEDIFCKKKYEAGRLLWVLND
                     QSGYDNSERDILEFLKKMTPNQKNGAVWGWLRADLQLLYDLRCLLRSEQLQFLGVMIA
                     HHHLFGMIDRDLMEYQRTREDVHLESALSLLGELGAAFKDKIGSPEGLELLEKHIKGF
                     EGVEDLTPGDLENIDLIRVYLSQARG"
     gene            complement(2654..>2878)
                     /locus_tag="PKK85_04450"
     CDS             complement(2654..>2878)
                     /locus_tag="PKK85_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25301.1"
                     /translation="ILSKLMEAIEYLHNKGLNGNIKRPENERVRVQYFKALCNACSVY
                     NQISQNLELDELKRDMDSLRKELKDHDKGG"
BASE COUNT          767 a          673 c          548 g          890 t
ORIGIN      
        1 aacgcctgag gggtgggatc cctgagagtc ttatgcagct gcaaaaacca ccctcagaga
       61 tattcccatt acctgaggat ctgatcttat tggaagatga aaccaccccg ggagaggtgg
      121 aaaaatgaaa agagaacggc ccgttctaag gatgtacgcc tccgataaga aggaactatt
      181 agaccctaaa agcctggagg gtttcaagaa aggggatccg atcgccttga tatacggccc
      241 ggacctcata agtttaattg atgacttgaa ggaactccta gaggattatc ttattttata
      301 tgagagatat gaggattgcc aggctgtaat taaaaagcat atacaaaaac aaggcggggg
      361 ttaaggggct tatgaataat gatgacatcc tggagactga acaccaagaa cggatccgtg
      421 atatacacca aaaaatcgat aacatagaat ataatataaa acggtcattt gaccttatga
      481 tcttatacct ccacctaaca aaccgggagg attgcagccc tgaatttaga cgggctatta
      541 atgattttat aggaaagtat ctcgaaaaat cagaacttag tatacctgag atacttgaaa
      601 gggaatatcc cgaacatgca gaataagtat aaaaaattgg gaggtggtgg agaggtagaa
      661 ttaaaactcg ttgaaggaaa aaaaactact ctctccacct aaaatggatg gaggcatgtt
      721 ttttaagggg ttcgtggagt aatgcccccg ctctccaggt cataaccccc aatttaggaa
      781 aaaaaaatat aattttatta tttaattctt tttgttaccg tatccatcac ggcctatatt
      841 cttattaaaa gatcgtggtt tcctgtaatc ccgtataatg ttcctaacat ctttaaagaa
      901 tttttggaag tccgttttag tgggataatc ttttttagtc atttttcttt ttcctccaat
      961 gtgtttaatt catgctccat agacttaacc cacccctcca ggtcccgccg cttagtgatc
     1021 aaactatcca attgcagatc ataagatgga cccctgaggg gcactacatc ccgccgggtc
     1081 aggggttcca ccacttggag ccgttgtttt atgtgctgga gttgtctatt agctactttt
     1141 aggttatgct ccaagtattc ccgccgtgtt gggaagtcat ggagtgtaaa gccttttttc
     1201 tggtagtggc cgggccgtgt ttgtttcatc ttgcttttat tcataaagga tcagttccac
     1261 cattattttt ttttatttga tcgcattggg gaccaacacc cggccggggt tccttttggt
     1321 cctcctggca accctcatat acttaagagc cggtggatta tccaagagct gggctatttc
     1381 aaatagcttt ttatcatact cggggttgtt ctcctggaag gcccgggctt gaagatccca
     1441 aaagaggcgg ttaagctcta tgacataggg aatatacacc tcaccttcca agttttggag
     1501 tgttgatccg ttgagttcgt ggtatttatc cctaatctcc tgggtttctt tttcagctgc
     1561 aactaataat ttttcaactt tctcgatctc tttaagctta gctttataat ccttatcaaa
     1621 taactcaggc ataaggcccg ccttttctct caggtgttcc gcctctcttt tcaggatgtt
     1681 taaaccatcc ttataacccc caattttatt agggccgtcc cgggttgctt tcgccttcaa
     1741 ttcatagatt tcagttctta aatcacttat ccgttccaat atttcggggt tcggttctgg
     1801 tagtttaatt ttatccataa ttaatcaact cctaattatt tatttttttt tatttgttac
     1861 ccctaagggt ctccttaatc ttctttttcg cctttccctc caccgcctcg acttcatgga
     1921 gtaatccaat taaaaaatcg tattccttta ggttttcggt ttggttaagc tccaatatta
     1981 gggcctcccg ggcctggagg gatttattca aagtctggcg ggctttatct gccctggttc
     2041 tctttcgggc ccggggccgt ctcttaaccc tatgcagcac ctgggatcaa cccctagcct
     2101 gggaaagata aacccggatc aaatcaatat tttccagatc ccccggtgtc aaatcctcca
     2161 caccctcaaa gcccttaata tgtttttcca gcaactccaa accctcagga cttccaatct
     2221 tatccttaaa ggcggctcct aattcaccta ataatgaaag ggcgctttcc aggtgcacat
     2281 cctcccgggt ccgttggtac tccatcaaat cccgatcaat catcccgaaa agatggtgat
     2341 gtgcaatcat aacccccaaa aattggagtt gttcacttct aagcaaacac cggagatcat
     2401 aaagcaattg caaatcagcc cggagccacc cccaaaccgc cccgtttttc tggtttggtg
     2461 tcatcttctt aagaaactcc aatatgtccc gttctgaatt atcataaccg ctttgatcat
     2521 taagaaccca taacaaccgg ccggcctcat atttcttctt acaaaaaatg tcctccattt
     2581 gctcggggct tatatcctcc accaggtcat catattcccg tctaagtttt cctattttag
     2641 gattcataaa acttcaacct cctttatcat ggtcttttaa ctctttccta agggaatcca
     2701 tatccctttt caattcatca agttccaggt tctggcttat ctgattatag acactacaag
     2761 cattacaaag ggccttaaag tattgcaccc gcacccgttc attctcgggc ctcttaatat
     2821 tgccattaag acctttatta tgtaggtact cgatcgcttc catcagctta gataatat
//
LOCUS       DAOQVW010000166         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_40800_length_2878_cov_3.531350, whole genome shotgun sequence.
ACCESSION   DAOQVW010000166 DAOQVW010000000
VERSION     DAOQVW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40800_length_2878_cov_3.531350"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..602
                     /locus_tag="PKK85_04455"
     CDS             102..602
                     /locus_tag="PKK85_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNS25302.1"
                     /translation="MKGKVWKFGDDVDTDIIIPGRYLVLTGEKELAAYVMEGCDPEFS
                     KKVEKGDIIVAGKNFGCGSSREHAPLAIKGAGVAAVVAESFARIFYRNSINIGLLLIE
                     SKDIAGRILEGDEIEIDMDNGTLKDLNSSEEFEIKPLPDFMLSIMEEGGLISYLRNHL
                     AEIKAQ"
     gene            681..1667
                     /locus_tag="PKK85_04460"
     CDS             681..1667
                     /locus_tag="PKK85_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNS25303.1"
                     /translation="MYKIAVIPGDGIGKEVMEATLHILEALDVEFDYQFAEAGDEYME
                     KTGVALPPETVDIVKNSQACLFGAAGESAADVIVKMRQELDLYVNLRPVKSYPGTNCL
                     FDNLDFVIVRENTEGLYIGREEYTDEGATALRVITRKASERICKFAFEYAKKTNRSKV
                     TAVHKANVLKKTDGLFKKIFYKVAKGYPDMELDDRYVDATAMFFITKPEMFDVIVTTN
                     LFGDILSDEGAGLVGGLGLIPSANIGENQGLFEPVHGSAPSHAGKGTANPSAMILSAV
                     LMLDYLEEHEEARRLEDALVAVLKEGKVVTVDLNGTASTMEMAQEVRRKLEV"
     gene            1751..>2878
                     /locus_tag="PKK85_04465"
     CDS             1751..>2878
                     /locus_tag="PKK85_04465"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNS25304.1"
                     /translation="MKTSDVRADIPLLEDIIYLDAASTTPTPRPVVEAMDEYYYHYNV
                     NTGRGTYALSLKATEKIENTRETIAKFFKASSEEIIFTKNTTEAINLVSRGFNFRKGD
                     KIVVSNMEHHSNLIPWLNLKKCGVEVEIVKANPKGVVNSSDVDDAIDKNTKLLTITHI
                     SNSMGAVQPVEELGKIALEKDILYLIDAAQSAGHLKLNVKDLMADFIAFPGHKGLLGP
                     VGTGFLYCDQGLQEKMEPLNLGGGTVDDVSQNGFLLTSCPNRFEGGTQNISGLIGLGE
                     AVNYVKRIGISDIEKHDRNLTRILYKRLKDLEGITVYGSPKNIYGIVSFNIDGINPHD
                     LAKILDETKNICVRSGQHCAILSTEHLGAREEGGTVRASFHY"
BASE COUNT          919 a          548 c          710 g          701 t
ORIGIN      
        1 cgctaaaaac tgaattaaat agagtagatg tttgattaaa cggccatagg tgcaaaacgt
       61 attgagatca actataacta ggtgatatta tggataagat cattaaagga aaggtctgga
      121 agtttgggga tgatgtggat acggatataa tcatccctgg cagatatctg gttttaactg
      181 gggagaaaga actggcggct tatgtgatgg agggatgtga tcctgaattt tccaaaaaag
      241 tagaaaaagg cgatatcata gtggctggaa aaaacttcgg ctgcggatct tcccgagaac
      301 acgcccctct agctatcaaa ggggcggggg tggctgcagt ggtggctgaa tcctttgcca
      361 gaatatttta ccgaaattcc attaacattg ggctgctctt aatagaatcc aaggatatag
      421 cggggcgtat cttagaaggg gatgaaatag agatagatat ggataatggg actctgaagg
      481 atttaaacag ttcagaagag tttgaaataa aacccttacc cgatttcatg ctgagtatta
      541 tggaggaagg gggacttatc agctacctga ggaaccacct ggcagagata aaagcccagt
      601 agaaatcagc catcacattt aatttgatgg tagtgaagtt aattggataa aaaattagtg
      661 aacaatggag gagtgtggtt atgtacaaaa tagcagttat acccggggat ggaattggga
      721 aagaagtgat ggaagccacc ctacatattt tagaagccct agatgtggaa tttgattacc
      781 agtttgccga ggctggtgat gaatacatgg aaaaaaccgg ggtggcattg cccccagaaa
      841 cggtggatat agtaaaaaat tcacaagcat gcctttttgg agcagcaggg gaatctgcag
      901 cggatgtaat tgtgaaaatg aggcaagaat tagacctcta cgtgaatcta cggcccgtta
      961 agtcttatcc tggaaccaac tgtctttttg acaacttaga cttcgtaatt gtaagagaaa
     1021 acaccgaagg actgtacatt ggaagagaag agtacaccga tgagggtgcc acggcattgc
     1081 gggttatcac cagaaaagca tcggagagga tttgtaaatt cgcctttgaa tacgctaaaa
     1141 aaaccaatcg gagtaaggta actgccgtgc acaaggccaa cgttctgaaa aagactgatg
     1201 gactcttcaa aaagattttc tacaaggtag ccaagggtta tccggatatg gagttagatg
     1261 atcgttatgt cgatgccacg gccatgtttt tcatcacaaa gccggaaatg ttcgatgtca
     1321 ttgtaaccac caacctcttt ggggatatat tatctgatga aggagccggc ttagtgggtg
     1381 gcctaggctt aataccatca gctaacattg gtgaaaacca gggtcttttt gaaccagtgc
     1441 acggctcggc acccagccac gcgggtaaag gcaccgccaa cccttcagcc atgatactct
     1501 cggcggtgtt aatgctggac tacctggaag aacatgagga agctaggcga ctggaagatg
     1561 cattagtggc tgtcctgaag gagggtaaag tggttaccgt tgatttgaat ggcacagcat
     1621 ccaccatgga aatggcccag gaggttagac ggaagctgga agtatagcta aatatccagg
     1681 ttataattaa atgggtccaa accatgggat aaataccaac tgacaattta acatgaaatt
     1741 tgaggcttaa atgaaaacat ctgatgtaag ggctgacatt cccctcctgg aagatatcat
     1801 ttatctggac gcagccagca ccacccccac ccctagaccg gtggtggagg ccatggatga
     1861 atactactac cattacaatg tcaacacagg ccgaggaact tatgccctat cactgaaggc
     1921 cacagagaaa attgaaaata cccgagaaac catagcaaaa ttttttaaag caagctctga
     1981 ggaaataatt ttcactaaaa ataccaccga agctataaac ttggtttcca ggggctttaa
     2041 ctttagaaaa ggtgacaaga tcgtggtttc caacatggaa caccactcca acctgatccc
     2101 ctggttgaac ctaaaaaaat gtggagtgga agtagagatt gttaaggcca acccaaaggg
     2161 tgttgttaac tcatcagatg tagacgatgc cattgataag aacactaaac ttttaaccat
     2221 cacccacata tccaattcca tgggagcagt tcaaccagtg gaggaactgg ggaaaattgc
     2281 cctggaaaaa gatattctct acttaattga cgctgcccaa tcagcaggtc acttaaaact
     2341 gaatgttaaa gatcttatgg ctgacttcat agctttccct ggtcataagg gtttgctggg
     2401 tccggtgggc actggatttc tttactgtga tcagggcctt caggagaaaa tggaacccct
     2461 gaatctgggt ggggggacgg tggatgatgt ttcacagaat ggttttctat taacctcctg
     2521 ccctaatcga tttgaaggag gaacacagaa cataagtgga cttattggct taggagaagc
     2581 agtgaactat gtgaaacgta ttgggatcag tgacattgaa aaacacgata gaaatcttac
     2641 ccgaattctg tacaagcgac tcaaagacct ggaaggaata accgtctatg gcagtccaaa
     2701 aaatatttat ggaatagtat ccttcaatat tgatggaata aacccccatg acctggccaa
     2761 gatactggat gaaacaaaaa atatctgcgt tcgaagtgga caacactgtg ctattttatc
     2821 cacagaacac cttggggcca gggaagaagg gggaacagtg agggcatcct tccactat
//
LOCUS       DAOQVW010000167         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_40894_length_2874_cov_2.960979, whole genome shotgun sequence.
ACCESSION   DAOQVW010000167 DAOQVW010000000
VERSION     DAOQVW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40894_length_2874_cov_2.960979"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PKK85_04470"
     CDS             <1..547
                     /locus_tag="PKK85_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall biogenesis protein"
                     /protein_id="HNS25305.1"
                     /translation="VEDASGALGDPSKRLAQGKQAHVILASTGSPKMVNAGSGGFFST
                     DREELVVDSKRITRALRASPLTCAAIKVEVQNASKSFSKTVKACNFLKKEFKNAFHRD
                     KRGVNVCLSTADPRKLAYHLRNQIEVLGGGMFTICPRYDRLNEPALCLEIKNLDLRCL
                     GSEKLGELVEMVDELLVKVEK"
     gene            complement(548..1039)
                     /gene="cgi121"
                     /locus_tag="PKK85_04475"
     CDS             complement(548..1039)
                     /gene="cgi121"
                     /locus_tag="PKK85_04475"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HNS25306.1"
                     /translation="MENNIQIAGFTAHIKNVSMVLSWTRKIAGQGTIQLLRAQRVAGE
                     KHLLHATYQALLAFQRRENISPDLGMEICLRASAQRQISRAIQLLGIAEGKQRVAAVL
                     VDCPEALPKLESMLGPQDTRALEFDPERLKAIYKISDQEVYAYKNVKKVLIERIALLN
                     LEK"
     gene            complement(1040..2536)
                     /locus_tag="PKK85_04480"
     CDS             complement(1040..2536)
                     /locus_tag="PKK85_04480"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNS25307.1"
                     /translation="MKVKIFDTTLRDGEQTPGVSLTPDEKLRIALKLDEMGVDVIEAG
                     SAITSPGEREGIKKVVGEELSAEICSFTRAIQMDIDAALDCGVDSVHLVVPTSDLHLE
                     HKLRKKPEEVKAMALESIQYAVDHGLLVELSAEDSTRSDMEFLKEIFRNGIRAGAQRI
                     CACDTVGMLTPEKSYDFYHELSQLKAPLSVHCHNDFGLAVANSLSGLRAGATQVHVTV
                     NGIGERAGNASLEEIVVALHTLYQVSTNVDISMLYDVSRMVARITGVFLQPNKAIVGE
                     NAFAHESGIHADGVMKKASTYEPITPELVGHKRRFVMGKHVGSHIIKQKVNEMGIRVD
                     EDRFNQIFQRIKSLGDMGKCVTDVDLQAIAEDVMGIMAEKPVELEELTIVSGNKVIPT
                     ASVKLKIDDMETLEAGVGVGPVDAALVAIKKSIADVIDIELEEYHVDAITGGTDALID
                     VVVRLRHDQQVVTARSTQPDIINASVEAYLSGINKILSDKRIEDKDPE"
     gene            complement(2622..>2874)
                     /locus_tag="PKK85_04485"
     CDS             complement(2622..>2874)
                     /locus_tag="PKK85_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01177 family methyltransferase"
                     /protein_id="HNS25308.1"
                     /translation="AVATDPPYGISASTGGEKSLDLYKQSIVSLAKIIKEDGRLCMAT
                     PHYLDIHEVLKGTNFEVIEQHHIRMHKSLTRVISILKQV"
BASE COUNT          739 a          680 c          620 g          835 t
ORIGIN      
        1 ggtagaagat gcatccggag ccctaggaga tccatccaaa aggctagccc aaggaaaaca
       61 agcacacgtt attttagcat ctactggaag cccaaaaatg gtgaatgcag ggagtggtgg
      121 gtttttttca accgacagag aggagttagt tgttgattca aagagaataa ccagagcttt
      181 aagggccagc cccctaacct gtgccgccat caaggtggaa gtgcaaaacg cgagcaaatc
      241 attttcaaaa acagtgaaag cctgtaattt tttgaaaaaa gagtttaaaa acgcgtttca
      301 ccgtgacaaa cggggagtga atgtttgttt gtccactgca gatcctagga aacttgctta
      361 ccatttacga aaccaaatcg aggtgctcgg gggtggaatg ttcaccattt gccccagata
      421 tgatagatta aatgaaccag cactgtgttt agagataaaa aatcttgatc tacggtgtct
      481 aggtagtgag aaactaggag aactagtaga aatggtggat gaattgctgg ttaaggtgga
      541 gaagtagtta cttttccaga tttaaaagag caattctttc tattaacact tttttcacat
      601 ttttataggc gtatacttcc tgatcagata ttttataaat tgctttcaac ctttcaggat
      661 caaattctag ggcacgagta tcctgggggc ctaacatgct ctccagttta ggaagtgctt
      721 ctggacagtc caccaacaca gcagctaccc tttgttttcc ctcagcaatg cctagaagtt
      781 ggattgcccg tgaaatctgt ctttgtgcag aagccctcag acagatctcc atacccaaat
      841 ccggggatat attttctctc cgctgaaatg ccaatagggc ttggtaggta gcgtgtaaca
      901 gatgtttttc tccggccact ctctgagccc gtagcagttg aatggttccc tgaccagcaa
      961 ttttcctagt ccaagataga accatagaaa cattttttat atgggcagtg aagcctgcaa
     1021 tttgaatatt gttttccatc tattctggat ccttatcttc gatgcgttta tcactgagaa
     1081 tcttgtttat tcccgagaga taagcctcca cactggcatt tataatgtcg ggttgggtgc
     1141 ttcgggcggt taccacttgc tggtcatggc gtaaacgtac caccacatct atcaaggcgt
     1201 cggttccacc agttatggca tccacgtggt attcttccag ttcaatgtct atcacatcgg
     1261 cgatactctt tttaatagct accaaggctg cgtccactgg acccacccct acaccggcct
     1321 ccagcgtttc catgtcatct atcttgagtt tgactgaggc agtaggtatt accttgttac
     1381 cagatactat ggtcagttct tccagttcca ctggtttctc agccattatt cccatcacat
     1441 cctccgcaat agcctgcaag tccacatcgg tgacacattt acccatgtca cccagggatt
     1501 ttatcctctg gaaaatctgg ttgaaacggt cctcatcaac ccttataccc atttcattta
     1561 ctttctgctt gattatatgg gatcccacat gttttcccat tacaaatcga cgtttatggc
     1621 ccactagttc aggggttata ggttcatatg tagaggcttt cttcataacc ccatcggcat
     1681 gaatccctga ttcatgggca aatgcatttt ctccgacaat ggccttgttg ggctgtagga
     1741 aaaccccagt gatacgagcc accatgcgtg acacatcgta gagcatgctg atgtccacgt
     1801 tggtggagac ctgatagagg gtgtggagag ccacaacgat ctcttctaga gatgcgtttc
     1861 ccgccctttc acctatacca tttacagtta catggacttg cgtagctccg gctcttaggc
     1921 cagagagact gttggccact gccaagccga agtcattgtg acagtgcacg gagaggggtg
     1981 ccttcagttg acttagttca tgataaaaat cataggattt ttccggggtg agcattccca
     2041 ctgtatcaca tgcacatata cgttgggcgc cggccctgat accattccgg aatatttcct
     2101 taaggaattc catatcactg cgggtggagt cttctgcgga caattctacc agcaacccat
     2161 gatcaactgc atattgaatt gattccaggg ccatggcctt cacttcctcc ggttttttgc
     2221 ggagcttatg ttccaagtgc aggtcagatg tgggcacaac cagatgaaca ctgtccactc
     2281 cgcaatccag ggctgcatca atgtccattt gaattgcccg tgtgaagctg catatctcgg
     2341 cagataattc ttcccccacc acctttttta tgccctctct ttcaccaggg gaagttatgg
     2401 ctgacccagc ttctatgaca tctactccca tttcatccag ctttagagct atccttagct
     2461 tttcatcagg agtaagggaa actccaggag tttgttcacc gtcacggagg gtggtgtcga
     2521 atattttaac cttcaagata atcacttcat caatttttga acaataaatc cattatggaa
     2581 gaaactttcc tccagggcca tgtaaacttt aacaaaccat gttaaacctg tttaagtatg
     2641 gatattaccc tagttaaact tttgtgcatt cttatatggt gttgttctat tacttcaaag
     2701 tttgtaccct tcagcacctc atggatatcg aggtaatggg gtgtggccat gcaaagccgt
     2761 ccatcctcct taatgatctt tgcaagggac acgatggatt gtttataaag atccagactc
     2821 ttctcacccc ctgtggaagc agatatgccg tagggtggat cagttgccac cgcg
//
LOCUS       DAOQVW010000168         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_41246_length_2858_cov_3.527292, whole genome shotgun sequence.
ACCESSION   DAOQVW010000168 DAOQVW010000000
VERSION     DAOQVW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41246_length_2858_cov_3.527292"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..436
                     /locus_tag="PKK85_04490"
     CDS             146..436
                     /locus_tag="PKK85_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF211 domain-containing protein"
                     /protein_id="HNS25309.1"
                     /translation="MAEGLIRIVLDILKPHDPILPHFATFLSKLRGVEGVNITLMEID
                     KETENIKVTMQGNDLDFDEISHAIEEYGGSIHSVDEVVAGRTMVEEVTTPQD"
     gene            442..1110
                     /locus_tag="PKK85_04495"
     CDS             442..1110
                     /locus_tag="PKK85_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HNS25310.1"
                     /translation="MPHHLMKISPRSVLEKFSSEEDLSESLMGEITTSHLSDAQKNLE
                     GSTRVVPGVKPIKNGVSIWGELVTAHTSSNDWGTVLKAIDQSDAGKILFLKSDNDNKA
                     IWGELTSKAAQNKKIKGTIIFGAVRDVSAVKSMDYPVFSRAIVPNAGTPLNEGEVNIP
                     IKIGDFTVEPGDFVLADDGGVVVVPRDKLQETLKESVIIKKTEEKILKQLNQGQFLSD
                     ILGI"
     gene            1123..2187
                     /locus_tag="PKK85_04500"
     CDS             1123..2187
                     /locus_tag="PKK85_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HNS25311.1"
                     /translation="MSDAFESIRKHKWKIIFTFAVSFFIIFAITFFIGIDEVIFTLEK
                     ASWEYILISLVLEAAILLIWTLRWKIILDVVDQAPKFSTILSMLFASLFGNNITPSAA
                     GGEPLRAYLLREVEGTPFELGFASSTADRVFEFLPFVLISIISAVFVLSWNLPLFTKF
                     IVTILIILSMILFGILIYAGLNRQVTQKIVIRLARSVYPFFMRLAKNPLTFGEVAEKL
                     VFYISRFSSGFKTAFKDRKVFIIGFILSFFMWGLDMLRMYVCFVSLGSFPPISPLIII
                     YTIGILISLLPLLPGAWGIREVILIGLFAVAGISADTVMAASLIDRLGSYVIPTIIGA
                     LAAFYYGKKVRNMEKKPSST"
     gene            2282..2566
                     /locus_tag="PKK85_04505"
     CDS             2282..2566
                     /locus_tag="PKK85_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HNS25312.1"
                     /translation="MKKLGNVLQISNRGRIILRSGQTPSFGLPVFTKKEKKLGHVHDI
                     FGPTKDPYISVKVYAKNSKNLDKRVGETLYIPSYSKKASKKWGRKKRSKK"
     gene            2623..>2858
                     /locus_tag="PKK85_04510"
     CDS             2623..>2858
                     /locus_tag="PKK85_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HNS25313.1"
                     /translation="MKQDMSEIENAETKCPECESTKLINDHERGEIVCGSCGLVIDDN
                     LVDMGPEWRAFDHEQRDKRIRVGASLTYTIYDKG"
BASE COUNT          902 a          507 c          589 g          860 t
ORIGIN      
        1 tagaaaacta atttagtctt taataattgt catattatga ggaatttatt caaaactgac
       61 aatgacatat ctataaatat cctgcatcag aaaaataaag acaccacaat gaattttaga
      121 ttataaattt gcggaggata taaaattggc agaaggccta ataagaattg ttttagacat
      181 attaaaaccc catgatccta ttttaccaca ctttgccaca tttttgagta aattaagagg
      241 cgtggaagga gtgaatatta ccctgatgga aattgataag gaaactgaga atatcaaggt
      301 caccatgcag ggtaatgact tggacttcga tgagatcagt cacgccatag aggagtatgg
      361 tgggtccatt cacagtgtag acgaagtggt ggctggaagg accatggtcg aagaagttac
      421 cacgccacag gattagatca catgccccac cacttaatga aaatttctcc cagatcagta
      481 ttggaaaaat tttcatctga agaagattta tctgaatctt taatgggtga aataactact
      541 tcccacttgt ctgatgccca gaaaaatttg gagggttcta ccagggttgt tcccggagtg
      601 aaacccatta aaaatggagt tagtatctgg ggggaattgg ttactgccca tactagttcc
      661 aatgattggg gaactgtgtt aaaagctata gaccaatctg atgctggtaa aattctgttt
      721 ttaaaatctg ataacgacaa taaggcaatt tggggtgaat taacttccaa agcagctcag
      781 aataaaaaaa ttaaaggcac cataatcttt ggagcagttc gggatgtttc tgcagttaaa
      841 tccatggatt atcctgtttt ttcccgtgcc atagttccca atgccggcac tcccttgaat
      901 gaaggagaag taaacattcc cataaaaatc ggagatttta ctgttgaacc aggagatttt
      961 gtcctagctg atgatggcgg ggtggttgtg gttccccgtg acaaactcca agaaaccttg
     1021 aaagaatcag tgatcataaa aaaaactgaa gaaaaaatct taaaacaact taatcagggt
     1081 caatttcttt ctgacattct gggcatttaa tcggggtttt agatgagtga cgcctttgaa
     1141 tctatacgta aacacaagtg gaaaataatt tttacctttg ctgtttcttt ttttatcata
     1201 ttcgccatta cattctttat tggaattgat gaagttatct ttacacttga aaaggccagt
     1261 tgggaatata tattgataag tttagtttta gaagcagcaa tattacttat ttggactcta
     1321 aggtggaaaa tcatactgga tgtggtggat caggctccca agttcagcac tattttatct
     1381 atgctatttg ccagcctgtt tgggaataat attactccca gcgcagcagg aggagaacct
     1441 ttacgggcct atctactgcg ggaagtggag gggacgccct ttgagttagg atttgcatct
     1501 tctactgctg accgggtgtt cgagttttta cccttcgttt tgatatccat catatcagcg
     1561 gtgtttgttc taagctggaa cttaccactc tttaccaaat ttatcgtgac catactaatt
     1621 atattatcaa tgatactctt cggaattctc atttatgcag gtttaaaccg acaagtaacc
     1681 caaaaaatcg ttatccgctt ggccagatca gtttatccat ttttcatgcg tttggccaaa
     1741 aatcccctga cctttggtga agtggctgaa aaactggttt tctatatcag tcgtttctcc
     1801 agtggattta aaactgcctt taaagataga aaagttttta ttatcggttt tattctctct
     1861 ttctttatgt ggggccttga catgttgcga atgtatgttt gttttgtttc attaggatct
     1921 tttccaccca tatccccctt gataataatt tacactattg gaattcttat atcactcctt
     1981 ccactacttc caggtgcttg ggggataagg gaagtgattt tgataggtct atttgccgtg
     2041 gcagggattt cagcagacac agttatggca gccagcttaa ttgatcgttt gggcagttac
     2101 gtcataccca cgattattgg tgctttagct gccttttact atggaaaaaa ggtaagaaac
     2161 atggagaaaa agccttcttc tacatgagtt gacaccccat accccttatt attaaaatta
     2221 tcgaaatatt tatatagtag ctcctagaga atgtagtata taaatatttc ggttaaactc
     2281 tatgaagaag ctgggaaacg tactgcaaat ttccaatcgg ggccgaatta tattgagatc
     2341 cggccaaaca ccctcttttg gtcttcctgt ttttacgaaa aaagagaaaa aattggggca
     2401 tgtacacgac atatttggac ctaccaagga tccttacatt tcagttaaag tctacgcaaa
     2461 aaattctaaa aatcttgata aacgagttgg agagacattg tatataccca gctactccaa
     2521 aaaagccagc aagaaatggg ggcgaaaaaa acgaagcaag aagtaatgga aaaaataccc
     2581 gaaaaagatg aactaaagac tgaaatttcg ggaaaagaac aaatgaaaca ggacatgtca
     2641 gaaattgaaa atgctgaaac aaagtgtcct gaatgtgaat ctacaaaact tataaatgac
     2701 catgagcgag gcgagatagt ttgtggaagt tgtggtctgg ttattgacga caatctggtg
     2761 gatatgggac ctgagtggag ggcttttgac catgaacagc gtgataaaag aatcagagta
     2821 ggagcatccc tcacctacac tatctacgat aaagggtt
//
LOCUS       DAOQVW010000169         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_42138_length_2819_cov_2.655933, whole genome shotgun sequence.
ACCESSION   DAOQVW010000169 DAOQVW010000000
VERSION     DAOQVW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42138_length_2819_cov_2.655933"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..445)
                     /locus_tag="PKK85_04515"
     CDS             complement(62..445)
                     /locus_tag="PKK85_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25314.1"
                     /translation="MRVSEGVPRGRVVKVYHPELFQEEEEVIVFSRAEFSRIYTSIER
                     QLDCIVKVDLHLDRSREWKLMGYWPRIMRSVHRIDQDMDLILRKEALQSYLDVYLHEP
                     VESSQILPVTGREHIPTRETPHLLY"
     gene            1232..1546
                     /locus_tag="PKK85_04520"
     CDS             1232..1546
                     /locus_tag="PKK85_04520"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNS25315.1"
                     /translation="MLRIKGIDEPPQEEDGFRILIDEFWPKNLSKKEAKVDLWLKELT
                     ITKNLHEWPDPPSYLTRIEKRYHELCRKKRIIKIIRDNEKERGTVTFLYSTIRSITHL
                     QL"
     gene            1712..2275
                     /locus_tag="PKK85_04525"
     CDS             1712..2275
                     /locus_tag="PKK85_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HNS25316.1"
                     /translation="MNCAVCLEKECGRGKDCLGNGEKIKKLYQEGEVSLIKAAAQVEA
                     RYYMKKTRLEETIIFAQKMNYKKIGLAFCVGLSDEAQAIYELFREHFQVYTVCCKICG
                     IDKDEFQLEKIDTGGGEAMCNPLGQAAFLNQKKTDLNVIIGLCIGHDILFSEHSNAPV
                     TTLAVKDRVLAHNPLGAIYSHYYQDKK"
     gene            complement(2284..>2819)
                     /locus_tag="PKK85_04530"
     CDS             complement(2284..>2819)
                     /locus_tag="PKK85_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25317.1"
                     /translation="YGNPDSADKIAIIVGVHPQEAQAHQVFIDSLKEHNSTLQGCYYI
                     YRVKVTRDVEDYQKGRDHGQVLARHYVVPDILKQDFDLVVDVHSNQGNYQLRRFIYLP
                     APSPTADRVASQVVKKIKWLSIYSPPNPTSPEYVTIPLIKSGIPAFIYETYLYEPYHQ
                     SKTQADQFISVLDTIQI"
BASE COUNT          811 a          629 c          637 g          742 t
ORIGIN      
        1 aggagtatcc tcctctggtc catgatgccg tagctgtaac gcattatcta ccaaaacccg
       61 cttagtacaa gaggtgggga gtctccctgg tggggatatg ctcccttcca gttactggta
      121 ggatctggga actttccact ggttcatgga ggtagacgtc caagtaggat tgtaatgctt
      181 ctttccggag tatcagatcc atatcctggt ctatcctgtg cacactccgc atgatccgtg
      241 gccagtaccc catcagcttc cactccctgc tccggtccag atgcagatcc accttgacta
      301 tgcagtccag ctgcctctct atggaggtgt aaatccggct gaactctgct ctgctaaaca
      361 caataacttc ctcctcttcc tggaagagct ccggatggta aaccttgacc actctccccc
      421 taggcactcc ttcactaact ctcatccact ccctccatgc tgtgtataac ctaggctagg
      481 ctataatctg agatttgtcc aagagcatct gagaagtaaa aaaaaattcc aggatccatc
      541 aggataaaaa aaagagccca ggtactctgg agtggagaaa accttcacta gatccttatt
      601 tttaactcag gctttagtat ggcttctgag accttgtctt ccaacttcat tacctgtaac
      661 ttcttggggg agttctactc agggagcccg ctggtcatcc cctgagttca agggccttag
      721 tatggatggg gagtttttcc gggttgcacc cttgacctac cccggcatgg tagaggaaag
      781 agccatcaac aagatatcta agactccatc agtggatggg tgatgaatat gtggggagtg
      841 gctttcccag tctggtttca tctagcttca gttcttagaa ttcccatttt tccacctaat
      901 ttttaagaaa gtttatttac tagttattac aattaagata ttagggtgcg ttggtttaag
      961 cggatataaa atcgatgtag agttgttgat atactgccct ccagaattag aaatagaaaa
     1021 caaaaaataa aaaaagaata taaatagaat cttaaaccac tgcaccccca ctctttttaa
     1081 gcccaccagt tggacctatt tctactattt ttatttgcaa tactactcgt ggcgtggaat
     1141 tatttcctag acaactatat atatcatgat taataatgtt agattatcta tcatgtttga
     1201 tcatgataat ttgtataggt gtgacagata tatgttgaga atcaaaggaa tagatgaacc
     1261 gccccaggaa gaagacggct tccggatatt gatagatgaa ttctggccca agaacttatc
     1321 aaaaaaggag gctaaagtgg atttgtggct taaagaacta acgataacta aaaatctcca
     1381 tgaatggccg gatcccccca gctaccttac aaggatcgag aagaggtacc atgaactttg
     1441 caggaaaaaa aggataataa aaatcatcag ggacaatgaa aaagaaaggg gaacagtgac
     1501 cttcctgtac tctaccatca ggagcataac gcacctgcaa ctttaatggg gatccttatg
     1561 cccacaccat ggtctacctg gatgtttcac ctccactggg ctccaaagaa atctgccaag
     1621 ccaccagtcc cggagatatt atccaaaaaa ttcaacaggg gatttaaact aaaaaaaagg
     1681 gatgattcag agatgaaaag gaggttctac cttgaattgt gctgtatgcc tagagaaaga
     1741 atgtggcagg ggaaaagatt gtctgggaaa tggtgaaaag ataaagaaac tgtatcaaga
     1801 gggggaggta tctctgataa aagccgcagc ccaagttgaa gcccggtatt atatgaagaa
     1861 aacgcggctg gaagaaacca tcatcttcgc ccagaagatg aactacaaaa aaatcggact
     1921 ggccttctgc gtgggcctgt cagatgaagc ccaggcaatt tacgaactat tccgggaaca
     1981 cttccaggta tacacggttt gctgtaaaat ctgcggcatt gacaaagatg aattccagct
     2041 ggaaaagatc gatacagggg gtggcgaggc catgtgcaac cccctgggcc aggcagcatt
     2101 tcttaaccag aaaaaaaccg acctcaacgt aatcatcgga ctttgtatag gccacgacat
     2161 actgttttct gaacattcaa atgcaccagt aaccactctg gctgtgaaag accgggtact
     2221 ggcccataac cccctgggtg cgatatattc ccattactac caggataaaa agtagagcca
     2281 tcattagatc tggatggtgt ccagcactga tatgaactga tcagcctggg ttttgctctg
     2341 gtggtatggc tcgtagaggt aggtttcgta gatgaaggcg ggtattcctg atttaattaa
     2401 agggatggtc acatattctg ggctggtagg gttaggtggg ctgtatatgg atagccactt
     2461 tatcttttta accacctggg aagccaccct atctgcagtt ggtgacgggg ctggtaggta
     2521 gatgaacctc cttagctggt agtttccctg gttggagtgc acatcaacca ccagatcaaa
     2581 atcctgcttc aggatatcag gcaccacata atgcctggcc aaaacctggc catggtccct
     2641 tcctttttga taatcctcca cgtcacgggt gaccttaacc ctgtaaatat agtaacaacc
     2701 ttgaagggtg ctgttatgct cctttaagga gtctatgaat acctggtggg cctgggcctc
     2761 ctgtggatgc acaccaacaa tgattgcaat cttatctgct gagtcaggat ttccatagg
//
LOCUS       DAOQVW010000170         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_42173_length_2817_cov_3.086169, whole genome shotgun sequence.
ACCESSION   DAOQVW010000170 DAOQVW010000000
VERSION     DAOQVW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42173_length_2817_cov_3.086169"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1290)
                     /locus_tag="PKK85_04535"
     CDS             complement(82..1290)
                     /locus_tag="PKK85_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNS25318.1"
                     /translation="MKRSHISIMNRSFQVLDQRRSKLLKDPKTLKLKERVQGIREYAM
                     DHLEELLEEAARNMEENGVEVIMAASGEEAREAILDSLEGEVLVAKSKSNTAGEILLS
                     EHLEEEGVEVLETDLGDRIVQMHQESRPSHPIGPAAHLDLEMISLIVSDYLDKEVESD
                     PRSILEAVKEDILQELPNIRVGITGANSVAAHDGALLMVHNEGNISLLSLLDVHIVLV
                     GIDKVVSTLEDAVSVVKLETIYATGKKTPAYMNVVSSPSKTADIEQELLKDMYGARRV
                     VVVFLDNGRREVLREGKESLWCIGCGSCIVNCPVYGVSGPEFGYRRHLGGRGVVMSRY
                     LEDEVACTDSGLYKCTLCGNCTRECPLEIPTNQIMELMRRECVEGGHQKEEHQVIRDN
                     IKKRGSAYRD"
     gene            complement(1287..1958)
                     /locus_tag="PKK85_04540"
     CDS             complement(1287..1958)
                     /locus_tag="PKK85_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019267076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNS25319.1"
                     /translation="MIYFQGCTARKKLKTIPKSTQKLLDQAGVEYKLLDDEECCGSVL
                     LRTGFAEDAQEAMRKTYDKLKGERVVVSCAGCYRTFREDYPQHVGEVDVVHISQLLEE
                     LISEGKLQIQKEEVKVTYHDPCHLGRHLGEYDAPRRVLEKKGDLVEMDKNREEARCCG
                     SGGGVKSAYPELSESMAERRVDDARKTWAELLVTCCPFCVLNLESVGKIRVLDLTEFL
                     LEDKL"
     gene            complement(1987..>2817)
                     /locus_tag="PKK85_04545"
     CDS             complement(1987..>2817)
                     /locus_tag="PKK85_04545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS25320.1"
                     /translation="LESKALNYSYDIGIATIWTSCYHILAHQDNFDYKMYFIQDFEPD
                     FYPHGDLYYLALNTYHFGFHMVSLGQWNKQRIEKIVNSNVDWVDFPVELDYYQMNRRK
                     LQIKDKVMITVYFKTDRKRAPFLLAEQIKYMQRKLKNSGYQVEVNLFGVSKVVKIPNT
                     TNLGKLVPHKLRELYKKSHIGVVASFTNISLVNYEMIASGLPVVDYRDGSAPTFFSEQ
                     EMIFVESDIKDLYNKINHYLHHQEDLNLILEKAQEKLEKDNTSWKKSAQQFNSILIKV
                     "
BASE COUNT          627 a          807 c          629 g          754 t
ORIGIN      
        1 ttctattcaa gtatcatgga taactccact atttattata tggcgggctg gggttgataa
       61 atccctaaca aaaaaaattt tctaatcccg gtaggctgat cccctcttct ttatattgtc
      121 ccggatcacc tggtgctcct ccttctggtg accgccttcc acgcactccc ggcgcattaa
      181 ctccatgatc tggttggtgg gtatctccag gggacactcc cgggtgcagt taccgcagag
      241 ggtgcattta tagaggcccg agtcagtgca ggccacctca tcctcgaggt aacggctcat
      301 caccaccccc cgaccgccca ggtgccgcct gtagccgaac tccgggccag acacaccata
      361 aactggacag ttcacgatgc aactcccgca gccaatacac cacaaactct ctttaccttc
      421 ccggagcacc tcccggcggc cgttatccag gaagaccacc accactctcc tggcaccgta
      481 catatctttt aagagttcct gttcaatgtc cgcggtcttg gagggggagc tgaccacgtt
      541 catgtaggcg ggggtcttct tgccagtggc gtagatggtc tccagcttca ccaccgacac
      601 cgcatcctcc agggtggaca ccaccttatc tatgcccacc agtactatgt gcacatccag
      661 taggctgagt aagctgatgt tgccctcgtt gtgcaccata aggagggccc cgtcatgggc
      721 agctacagag ttggccccgg tgatccccac ccggatattg ggtagttcct ggaggatgtc
      781 ctccttcaca gcctccagga tgctgcgggg atcactctcc acctctttat ccaggtagtc
      841 agagacgatc aggctgatca tctccaggtc caagtgggcc gcagggccga tgggatgtga
      901 gggccggctt tcctggtgca tctgcacgat ccggtccccc aggtccgtct ccaggacctc
      961 cacaccctcc tcctccaggt gctccgagag gaggatctcc ccggcggtgt tggacttgga
     1021 cttggccacc agtacctcgc cttctaagct gtccaggatg gcctcacgtg cctcctcgcc
     1081 actagcggcc atgatgactt ccaccccgtt ctcctccatg ttacgggccg cctcctccag
     1141 gagttcctcc aggtgatcca tggcgtactc ccggatcccc tgcaccctct ccttcagctt
     1201 cagggtcttg gggtccttta ataacttgct ccgtctctga tccagaacct ggaaggaacg
     1261 gttcatgatg gaaatgtggg accttttcat aatttatcct ccagtaggaa ctcggtcaaa
     1321 tccaggaccc gtatcttacc caccgactcc aggttcagga cacagaaggg gcagcaggtg
     1381 actaaaagct ccgcccaggt cttccgggcg tcatccaccc tccgctccgc catactctcc
     1441 gataactccg ggtaggcgga tttaacccct ccaccggaac cgcagcaacg ggcctcctcc
     1501 cggttcttat ccatctccac caagtctccc ttcttctcca ggacccggcg gggggcatcg
     1561 tactccccga ggtgccggcc caggtggcag gggtcatggt aggtcacctt aacctcctcc
     1621 ttctggatct ggagcttacc ctcggatatt aactcctcta atagctgaga gatgtgcact
     1681 acatccactt cccccacatg ctgggggtaa tcttccctga aggtgcggta gcagcctgcg
     1741 cagctcacca ccaccctctc ccctttcagt ttatcataag tctttcgcat ggcttcctgg
     1801 gcatcctcgg caaagccagt gcgcagtaaa actgagccac aacactcttc atcatccaat
     1861 aatttatatt ccacccctgc ctggtccagt aacttctggg tggatttggg tatcgttttt
     1921 agcttttttc gggcagtgca gccctggaag tagatcatgg tttatttcct taagttaatc
     1981 agtcttttac actttgatca ggatactgtt aaactgttgg gctgattttt tccagctggt
     2041 gttatccttt tccaactttt cctgggcttt ttccaggatc agatttagat cctcctggtg
     2101 atggaggtag tggttgatct tgttgtaaag gtccttaata tctgattcta caaaaatcat
     2161 ctcctgttcg gaaaagaaag taggggcact tccatcccga taatcaacca ctggaagtcc
     2221 ggaggcgatc atttcataat tcaccaggga aatattggtg aaagaagcta ccactcctat
     2281 atgggacttt ttatacaact ccctgagttt atgggggact agtttaccta aattagtggt
     2341 gttgggaatc ttaaccacct tactaacccc aaatagattt acttcaacct ggtatcctga
     2401 attctttagt ttccgctgca tatatttaat ctgttcggcc agaagaaaag gtgctctttt
     2461 acgatcagtt ttaaaataaa cagttatcat tactttatct tttatctgta attttctacg
     2521 gttcatctgg taataatcaa gttccactgg gaaatccacc cagtccacat tagagtttac
     2581 gattttctca atccgttgct tattccactg acccagacta accatgtgaa agccgaaatg
     2641 gtaggtattc agtgccaggt agtaaagatc accatgggga tagaaatccg gttcaaaatc
     2701 ctggataaaa tacattttat agtcaaagtt atcctggtga gccaaaatat gataacagga
     2761 agtccagata gtggcaattc ctatatcata actgtagttc agtgctttac tttccag
//
LOCUS       DAOQVW010000171         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_42495_length_2803_cov_2.674309, whole genome shotgun sequence.
ACCESSION   DAOQVW010000171 DAOQVW010000000
VERSION     DAOQVW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42495_length_2803_cov_2.674309"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKK85_04550"
     CDS             complement(<1..255)
                     /locus_tag="PKK85_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25321.1"
                     /translation="MEIDNPLQMNDWNIKPFLWLIFLLQFSLLILILLESIDIQIPIL
                     RELISFIILFFIPGVLILRILRLHDLGNTRTVFYSVGLSIV"
     gene            complement(257..1384)
                     /locus_tag="PKK85_04555"
     CDS             complement(257..1384)
                     /locus_tag="PKK85_04555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS25322.1"
                     /translation="MKILLVNHSEITSPGGVHKNIVEISKNLSKRGHQVTVLQSNTMN
                     LPNHEFYEGFEIIRIKSWLADAFYGLNPKIFFYLKSQLKELNPDVVHVHGYHTLFSPE
                     VMYLIKQFDLKIPVVFSPHFGIFSRTSFAGKYFWEPYKNLIGRKIFNYCDFIIAASEF
                     ELNSLITNFNLTRKNIKIIPHGVNFTWSKKSKKTDDKLNLLYVGYLLKIKGVQYILEA
                     VHELVYKKKMNLLLNIVGDGSYESELRKLANELDIDEYINWKGFIDFSQPEKLMNFYK
                     EADILLLLSESENYGIVVSEALAMGTPVLVTKTTALNEFLNEPGCFGVEFPPNPLEVA
                     RLVKEIYKNEVQVGPFSSKVQNWETVSDAYESVYFSLIKGT"
     gene            complement(1399..2304)
                     /locus_tag="PKK85_04560"
     CDS             complement(1399..2304)
                     /locus_tag="PKK85_04560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS25323.1"
                     /translation="MENENVGEHVIMECPLVSVVIPTLNSEKTLEACLDSVGGQTYPN
                     IEIVVVDGGSVDRTVDIATATGAKVINANVQSMTKQTNIGINGSHGKYLYRVDSDVIL
                     DPDIVESSVNKCETEGYDGVCIFWLPDESISFWAKIRKIEKESYIEHPNYVGATKYNK
                     NVLGARFLRRDVLENIGGFDEEIPTLGEDYALYNKLAKSDFNFALINSRERHIGEPRK
                     IKDIIRKNFRYGTALTAFIKEQKGGLRQFAPTGRTYLKTAFKRAFKKDFKLFLGLIIY
                     IFTVYASTATGMVYYRVTKKTKRVA"
     gene            2621..>2803
                     /locus_tag="PKK85_04565"
     CDS             2621..>2803
                     /locus_tag="PKK85_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HNS25324.1"
                     /translation="MKKNESKLQKAFKDKSKNQNIISIILVAILFVFIAITVYLIISP
                     VQPEPFTEFYLLGPDGK"
BASE COUNT          913 a          536 c          425 g          929 t
ORIGIN      
        1 tacgatactt aaacctacag aataaaaaac cgttcgggta tttcctaaat catgtagcct
       61 gagaattctc aggataagaa cccccggtat aaagaaaaga attatgaaag aaattagctc
      121 tcttagaata ggtatctgaa tgtctatcga ttctaacaat atcaaaatta acaaagagaa
      181 ttgaagtaag aatataagcc aaagaaatgg cttaatattc caatcattca tttgtagagg
      241 attatcaatc tccattttat gttcccttaa ttaaactgaa ataaacgctt tcataggcat
      301 ctgaaacagt ttcccagttc tggaccttag aactgaaagg acctacttgt acctcatttt
      361 tataaatttc ttttaccaaa cgtgcaacct ccagggggtt agggggaaat tcaacaccaa
      421 aacaaccagg ttcattcaaa aattcattta acgccgttgt ttttgtaact aaaacgggag
      481 ttcccatggc taaagcttct gaaactacga ttccatagtt ttctgattca gagagtagca
      541 acaggatatc ggcttctttg taaaaattca tcaacttttc cggttgtgaa aaatcaataa
      601 aacctttcca gttaatgtat tcatctatat ccaattcatt ggccaatttc cttagctccg
      661 attcatagga tccgtcacca acaatgttca gtaaaagatt catctttttt ttataaacta
      721 actcatgtac tgcttctaaa atgtactgca caccttttat ttttagtaaa taacccacat
      781 ataacagatt taatttatca tcggtctttt tagatttttt ggaccaagta aaattgacac
      841 catggggtat aatttttata ttctttctag tcaggttaaa atttgtaatt aggctattta
      901 actcaaattc cgatgctgca atgatgaagt cacagtaatt aaatattttc ctaccaatca
      961 gattcttgta aggttcccag aaatattttc cagcaaagga tgtacggctg aaaattccaa
     1021 aatgcggaga aaagactact gggattttca aatcaaattg tttaattaaa tacattacct
     1081 ctggagagaa taaggtatga taaccatgga cgtgtactac gtcaggattg agttctttta
     1141 actgactttt aagatagaaa aagattttag gatttagacc gtaaaatgca tcggccagcc
     1201 atgatttgat ccggattatc tcgaatcctt cataaaactc gtgatttggg aggttcatag
     1261 tgttactttg gagtacagtt acttgatgtc ctctctttga aaggtttttg gatatctcta
     1321 caatattttt gtgcacgcct ccaggggagg tgatctccga atggtttact aaaagaattt
     1381 tcattacaac tcccccatct aggctactcg tttcgttttt ttcgttactc ggtaataaac
     1441 cattcctgta gccgttgatg cgtaaacagt gaaaatatat atgataaggc ccaaaaaaag
     1501 tttaaaatcc tttttaaacg ctcttttaaa tgcagttttt agatatgttc tccctgtagg
     1561 ggcaaattgt ctcagaccac ctttttgttc ctttataaat gcggttagtg ccgttccata
     1621 tctgaaattt ttcctaatta tgtccttgat ctttctgggt tctcctatgt gcctttccct
     1681 ggaattaata agggcaaaat tgaaatcact cttagctaat ttattgtata gagcataatc
     1741 ttcccctaaa gtaggaattt cttcatcaaa gccacctata ttttctagaa cgtcgcggcg
     1801 taaaaatcta gctcctaaga catttttatt atatttagta gcacccacat aattgggatg
     1861 ctcaatgtaa ctttcctttt cgatttttcg aatctttgcc cagaaactga tggattcatc
     1921 aggtaaccag aatatgcata ctccatcata tccttcggtt tcacatttat ttacacttga
     1981 ttctacaata tcaggatcca agataacatc agaatcaacc ctgtacaggt attttccgtg
     2041 agagccatta attccaatgt ttgtctgttt tgtcatgctt tgtacgttag catttattac
     2101 tttagctcca gttgcagttg ctatatctac agttcggtca acagatccac catccactac
     2161 tacaatctct atattgggat aggtttgacc tcccacacta tccaagcatg cttctagggt
     2221 tttctcagaa tttaaagtag gtataaccac tgaaacaaga ggacactcca ttataacgtg
     2281 ctccccaaca ttttcattct ccaaaccacc tcagctaaat ttaatttttg attaataaga
     2341 aatattactg gatataaaca aatatcatac taaaaaacct cagcttcaaa aatattttgg
     2401 attacctatt ttaaatattt tgttagtgca aagcaaaatt gatatactct aagaatttag
     2461 tacttattgt gcagtaaaca gctaatcatc tccatcaaca ttatgagata atagttctga
     2521 aaatcaataa tatagatgaa ataatcgtgt ccaccccata attcttggat atgcaaaaaa
     2581 attagctgtt tagtgaatta aaacactaaa aatacgaacc atgaaaaaaa atgaaagtaa
     2641 acttcaaaag gccttcaaag acaaatccaa aaaccagaat atcatttcaa taatcctcgt
     2701 tgccatccta tttgtcttca tagctattac cgtgtatctg ataataagtc cagtacaacc
     2761 agaaccattt actgagtttt atctgctcgg gccagatggt aaa
//
LOCUS       DAOQVW010000172         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_42573_length_2799_cov_17.604956, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000172 DAOQVW010000000
VERSION     DAOQVW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42573_length_2799_cov_17.604956"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKK85_04570"
     CDS             <1..330
                     /locus_tag="PKK85_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25325.1"
                     /translation="DGGEYVILPVQVDDMPVLFNIIDKRVKASKKTKKNKKDEDTGQE
                     FIKACGADLQELIGRTVVNIKTGEPLPLKYRNPGNMIELMGKIMKITMPNSSKEAEGE
                     DTPLEQS"
     gene            378..611
                     /locus_tag="PKK85_04575"
     CDS             378..611
                     /locus_tag="PKK85_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25326.1"
                     /translation="MNGVSPEMVGRFYYPDFSNYISYYSDPRGFLEHESLDEGVAMQK
                     LATYHDIQQDIIRKAEAKKREPTSSGVHKDEPK"
     gene            779..>2799
                     /locus_tag="PKK85_04580"
     CDS             779..>2799
                     /locus_tag="PKK85_04580"
                     /inference="COORDINATES: protein motif:HMM:NF021648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HNS25327.1"
                     /translation="MADKELGITVTGNLSDIEAKIQELTSLLNNINDVKIDANMDTES
                     IKADIESLQGALNELDNEVITPEVATEGISDVSTEAESAKGDMEDLGESGEEAGSMIE
                     GGMAVASLAVLALGADVEETAQKLGDLSVSYSKLGSSFEGGSAALRDQVANITDTEFP
                     EDEALTYISLLKRMGVAQEDLVGSADDLNKIRSATQSSVSDMTPLLQGLRAMNYDVSN
                     LEGAYNALYFANAKYVGGLPQFSSDLMRYSSYFNEMGFTIDQAAVVLAQAQKKWPNPR
                     EMRTNLKKAVDESDGDLSKFASSLGMTTDQIINADSTTGQYAGKIEQVSQQYEQTATT
                     TQKVDAATADLNTRYGDITKTLYDAGGAVYSFSKGIAEAWLIAKAAFKVGILEEDYAA
                     KFIEKPLMKGVDIIKGFGEKARPLMDDIGKILTKPFTKTKVPELISPEMLSGVDDGFA
                     QMEAKIAQKSGGFTDDLVKTLQKAVPKVKVPAGLIPEGIGSTITKGIGRIAPKLLGEG
                     ATVGILFGQGMYNALDNTKKGLGTLIVEMYSLDILKNIPVLGDMVSQAEWFLNLIGTP
                     EQWASVLIGKQNVEKMQAWLAGVQDWTRQSWDWIVAWVMNLPSAIGEAATGIYDSAVS
                     LWNEFIKGFTEAFSPVTTLLSDAETKIKDGFQWLIDLPGKAWQWGWDII"
BASE COUNT          856 a          530 c          718 g          695 t
ORIGIN      
        1 gatggggggg aatatgttat cttaccagtt caggtagatg atatgccagt cctattcaac
       61 atcatcgaca aaagggtcaa ggcatctaag aaaaccaaga agaacaaaaa ggatgaagac
      121 actgggcagg aatttattaa agcctgtggt gctgatttac aagaactaat aggtagaact
      181 gttgttaata ttaaaactgg tgaaccatta cctctcaaat accgtaaccc tgggaacatg
      241 attgaattaa tgggtaagat catgaagata accatgccca actcatctaa agaggctgag
      301 ggagaggata cccctttaga acagtcctag aattaaagag ggaactattt gcaggtaagg
      361 catttttatt agggaaaatg aatggtgtgt ccccggagat ggtgggccgg ttttattatc
      421 cggattttag taattatatt tcttattact cggatccacg gggcttcctt gaacatgaat
      481 ctctggatga gggtgtggct atgcagaaat tagctactta tcatgacatc cagcaggaca
      541 ttatcaggaa ggcagaggcg aaaaagaggg aacctacttc ttccggtgtt cataaagacg
      601 agccaaaata ataaatatcc ctaataggag gaaccagggg cctatgaatg cactaactcc
      661 cacggtgaat agtatgatga ctattaattc ctgggtgaaa cttcgggggg tcttttcatt
      721 ttccatatca tagtatatta ttttttcatt aatttaaact ttacggagca aataacacat
      781 ggctgataaa gaactcggca tcacagtaac tggtaactta tcggatatag aggccaaaat
      841 ccaggaactc accagcctac ttaacaatat caatgatgtt aagattgatg ctaacatgga
      901 cacggagtcc ataaaagcag atattgaatc cctccaagga gcacttaacg aattagataa
      961 cgaagtcata acccccgaag tagccaccga aggcataagt gacgtatcca cagaggctga
     1021 aagtgccaaa ggagatatgg aagaccttgg cgaaagtggg gaggaagccg ggtcaatgat
     1081 tgaggggggt atggcagttg ccagcctcgc cgtcctagcc ctgggtgctg atgtggaaga
     1141 aaccgcccag aaattagggg accttagtgt ttcttattct aaacttggat caagttttga
     1201 gggaggaagt gcggccctcc gggaccaagt ggctaatatc actgacaccg agttcccaga
     1261 agatgaggcc ctaacttata ttagcttatt aaagaggatg ggtgttgccc aggaggatct
     1321 tgtaggaagt gctgatgacc tgaataagat caggagtgcc acccaatcta gtgttagtga
     1381 tatgaccccc ttactccagg gactccgggc tatgaattat gatgttagca accttgaagg
     1441 tgcgtataat gccctgtatt ttgctaatgc taaatatgtt ggggggttac ctcaatttag
     1501 tagtgacttg atgaggtaca gttcttactt taatgagatg ggattcacca ttgaccaggc
     1561 agcagttgtc ttggctcagg ctcagaagaa atggcctaat ccccgtgaga tgagaactaa
     1621 tcttaaaaag gcagtggatg agtctgatgg ggatttatcc aagtttgcaa gcagcttggg
     1681 gatgaccacg gaccagataa taaatgcaga cagtactacc ggccagtatg cggggaagat
     1741 tgaacaagta tcccaacagt atgaacaaac cgcaacaacc actcagaagg ttgatgctgc
     1801 tactgctgac ctcaacacca ggtatggaga cataactaaa acactctatg atgctggggg
     1861 tgcggtttac agtttctcta aggggatagc tgaagcttgg cttattgcta aagccgcctt
     1921 taaagtaggg atactggaag aggactacgc agctaaattc attgagaaac cattaatgaa
     1981 aggagtggac ataatcaaag gcttcggaga aaaagcccgg cccttaatgg atgatattgg
     2041 gaagatcctc accaaaccct tcaccaaaac caaagttccc gaacttatca gccccgaaat
     2101 gctcagtgga gtggatgatg gattcgccca gatggaagcc aaaatcgcac agaaaagtgg
     2161 gggattcaca gatgaccttg tgaagacact ccagaaggca gtgccgaagg ttaaagtccc
     2221 cgctggttta atcccagagg ggatcggatc cactatcacc aagggcatag gacggatagc
     2281 tcccaaactg ttaggtgaag gggccactgt ggggatactc ttcggccagg ggatgtataa
     2341 tgctctggat aatactaaga agggattggg taccttaatt gttgaaatgt acagtttgga
     2401 tattcttaaa aacatcccag tattagggga tatggtaagt caagctgaat ggttcctaaa
     2461 tctaataggc acccctgaac aatgggccag tgtcctaata ggtaaacaga acgttgaaaa
     2521 gatgcaagct tggcttgcgg gggtgcagga ctggactcgg cagagttggg attggatagt
     2581 ggcctgggtt atgaacttac cctcagctat tggggaagct gcaacaggga tttatgatag
     2641 tgctgttagt ttgtggaatg aatttattaa gggattcacg gaggcattca gcccagttac
     2701 tactctttta agtgatgcgg aaaccaagat taaagatggt tttcaatggc ttattgacct
     2761 ccctggtaag gcgtggcagt gggggtggga tattataga
//
LOCUS       DAOQVW010000173         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_42624_length_2797_cov_3.259664, whole genome shotgun sequence.
ACCESSION   DAOQVW010000173 DAOQVW010000000
VERSION     DAOQVW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42624_length_2797_cov_3.259664"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(451..708)
                     /locus_tag="PKK85_04585"
     CDS             complement(451..708)
                     /locus_tag="PKK85_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25328.1"
                     /translation="MWILESFLIKEVDLNLASVTVAAIYTSLFKALFVMCLTHMADEK
                     SFKATFEFKEVFSFFKDVGVDEISDVNSIHDFVRWINQLIN"
     gene            1130..1960
                     /gene="hisF"
                     /locus_tag="PKK85_04590"
     CDS             1130..1960
                     /gene="hisF"
                     /locus_tag="PKK85_04590"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNS25329.1"
                     /translation="MLAKRIIPCLDCDLQVPHGRVVKGVEFKQIRYAGDPVELATKYY
                     EDGADEIVFLDITASHERRETMAEVIKATTENVFVPICVGGGIRKPEDYVTMLKAGAD
                     KCSINTAAIHNPALIKQASEIVGSQACVVGIDAKRRYVDNPKEDDNRIIIETENGYCW
                     YDCSIYGGRDFTGKDAIKWAVECQETGAGEILLTSMDRDGTKDGYDLPLIRVMSEMLD
                     IPIIASGGGGNPEHIYRVFKDGKADAALAASIFHFNEYPVTVVKEYLQNKGVEVRIQD
                     "
     gene            1973..>2797
                     /locus_tag="PKK85_04595"
     CDS             1973..>2797
                     /locus_tag="PKK85_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25330.1"
                     /translation="MSLVINSTTPEGIILAADSRQSYRNMKGMARVGSDTASKLFQIN
                     CRIGVGTAGLAFLPEGGVVKNVSRFIEEYKRDPDVEDLEVEEAAQGLYDLFNQRYNWK
                     AQLNELEGKISQDLHMKGLRILEVQKTDNQIKFTYQNPQGMMEDVTAALDPLELLVAG
                     YNRDGSHQVFSCRIPGSVQKLRDSYQLDIQYGSSWIGQGDVVTRVVLGFDGRIANLDF
                     MKEIFQSKGESEVKRQLQGLEYAIQWGVMSLQDAIDFSTLMIQTTSAMQRFSDGINA"
BASE COUNT          893 a          590 c          613 g          701 t
ORIGIN      
        1 caatacccac ataatgactc ccattattac ataaattatt attacccccc cctaagttta
       61 atattatttg tgcatgggta ttattacgat ttaatattgt taatatatga tgctattttt
      121 ctgtaaaaac tgtggaataa actaaaactt atacaaaacg cttgaataga atttaaaaga
      181 taaaaaggcg ttgtattctt catttttaaa ttaactcact attatgcatg cttacgtgtg
      241 gagaaattat catgaatttt taatttaaat ccaaattaat ttctaaaatc ccacaagtcc
      301 aaataacctc ttcaaattag attatcttgg tacaataagc ccgtttgtcg accttaaaag
      361 ctgaataaat atgcgtaagc cagcaaagtt acagccacaa gaattggaat ccatcccaga
      421 gctggtagat ccaggaccag aggaatcaat tcaattaata agttgattaa tccaccgaac
      481 aaagtcatga atactattaa cgtcagatat ttcatccacc cccacatctt tgaaaaagct
      541 aaaaacctct ttaaactcga atgtggcttt aaaacttttt tcatcagcca tatgggtcag
      601 acacatcaca aaaagagctt tgaaaagaga agtgtagatg gcagccacag taactgaggc
      661 cagattcagg tccacctctt ttattagaaa ggactctaaa atccacacaa gaccataaaa
      721 aataattcca aatatcaaaa tcattccagg aatattaaac cctcccggaa cacgttcttc
      781 caatcattaa atcctggatg cccatcttca cctttaatgg tattagatac tatttttaat
      841 aggtaatcca aggccagaat tccaggaaca tttagaatct ggttgaaaaa aggattaaca
      901 atcccagata actttttatt tctaacaggt aagttaaaga atccaaaacg attttatcta
      961 gtttattatt tatggtcttc aaaaaatatt ccccaatttt ttgcctatta attattagat
     1021 cttccggttt taaaatataa agcctaatat agtgctttaa aaaattaaaa tatcaagaat
     1081 tgtttgatct tatattaatt aaattcctga gaaaaagata aggagccaca tgttagccaa
     1141 aagaatcata ccctgtttgg actgtgatct gcaggttccc catggacggg tcgtgaaggg
     1201 agtggagttt aaacagatcc gctacgcagg ggacccagtg gaactggcca ccaagtatta
     1261 cgaagatggg gcggatgaaa tcgtgttcct ggatattacc gcctcccacg agcgcaggga
     1321 gaccatggcc gaagtgatca aggccaccac tgaaaacgtg ttcgtgccca tctgcgtggg
     1381 gggtggcatt cgcaaaccag aggactatgt gactatgctc aaagcgggtg ccgacaagtg
     1441 ctccatcaac accgccgcca tccataaccc agctctgata aagcaggcct ccgaaattgt
     1501 gggcagccag gcctgcgtgg ttgggatcga tgccaaacga aggtacgtgg acaaccctaa
     1561 agaagatgat aaccgaataa tcatagaaac agagaacgga tattgctggt atgattgcag
     1621 catctatggt gggcgtgact ttacagggaa agatgccatt aaatgggctg tggagtgcca
     1681 ggaaacgggg gctggtgaaa tattactgac ctccatggac cgggacggga ccaaagacgg
     1741 ctacgacctt cccctgatcc gtgtcatgag tgagatgctg gacattccca tcatcgcatc
     1801 tggtggtgga gggaacccag aacacatata ccgtgtattt aaagatggta aagcagatgc
     1861 cgcattggca gccagtatct tccatttcaa cgaataccct gtgacagtag taaaggagta
     1921 tctgcaaaat aaaggagtgg aagtaaggat ccaggattag ggaggagatg aaatgtcact
     1981 ggtaatcaac agcactacac cagagggaat tatcttagcc gccgacagcc gccaatctta
     2041 tagaaatatg aagggaatgg cccgggtagg tagtgatact gcctctaaac tattccagat
     2101 aaattgcagg ataggagtgg gcactgctgg attggctttc ctccctgaag gaggggtggt
     2161 taagaatgtc agccggttca tcgaggaata taaacgcgac ccggatgtag aggacttgga
     2221 ggtagaagaa gcagcccagg gattgtacga cctcttcaac cagaggtaca actggaaggc
     2281 ccagctcaac gaactggaag gtaaaatctc acaggatctg cacatgaagg gtctgcgtat
     2341 cctggaggtc caaaaaacag ataatcaaat caaattcact taccagaatc cccagggaat
     2401 gatggaggat gttaccgctg cattagaccc cctggaactc ctagtggctg gttacaatag
     2461 agatggctcc caccaggtct tcagttgccg catcccagga tcggtgcaga aactgcgtga
     2521 cagttaccag ctggatatac agtatggcag ttcctggatc ggacagggtg acgtggtaac
     2581 ccgcgtcgta ctgggctttg atgggcgcat cgcaaacttg gacttcatga aagagatttt
     2641 ccagagcaaa ggggaatctg aagtcaaaag acaacttcag ggcctggaat atgccataca
     2701 atggggggtt atgagtctcc aggacgccat tgacttctcc accctgatga tccagaccac
     2761 cagcgccatg caacgctttt ccgatggcat aaacgcc
//
LOCUS       DAOQVW010000174         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_42757_length_2791_cov_4.256579, whole genome shotgun sequence.
ACCESSION   DAOQVW010000174 DAOQVW010000000
VERSION     DAOQVW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42757_length_2791_cov_4.256579"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKK85_04600"
     CDS             complement(<1..638)
                     /locus_tag="PKK85_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS25331.1"
                     /translation="MKIRSPIVSVLGHVDHGKTTLLDYIRGSFIAQKEAGGITQHIGA
                     TEIPMEAIESICSQFIQKLEIRESLPGLFFIDTPGHEAFTTLRKRGGALADLAVLMVD
                     LNEGFKPQTYEALNILKMYQTPFVVGANKIDRIPGWHIHEKEPFNKTYNKQNPQVQVA
                     LDNQVYELVGILHQEGFESERFDRVRDFARQVSIIPISTKTGEGIAELLTMLL"
     gene            complement(720..1178)
                     /gene="ndk"
                     /locus_tag="PKK85_04605"
     CDS             complement(720..1178)
                     /gene="ndk"
                     /locus_tag="PKK85_04605"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNS25332.1"
                     /translation="MKEKSFVMLKPDAVLRRLTGKILTRFEERGLEIVAAKMMVIPRE
                     LAQQHYAEHEEKPFFTDLVEYITSGPVLATVIEGEECITLIRKMVGATNPKEADTGTI
                     RGDYAIDTGRNIIHASDSPASAEREIGLFFKMDEICDCSFPDREWIQEEP"
     gene            complement(1175..1336)
                     /locus_tag="PKK85_04610"
     CDS             complement(1175..1336)
                     /locus_tag="PKK85_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HNS25333.1"
                     /translation="MRTCSFCGEEIDEGTGKMFVKKDGTVFFFCSSKCEKNRIKLGRV
                     PRKVKWVKQ"
     gene            complement(1353..1559)
                     /locus_tag="PKK85_04615"
     CDS             complement(1353..1559)
                     /locus_tag="PKK85_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HNS25334.1"
                     /translation="MEDGTPAEVIEVLKRTGMTGEVMQVKCRILDGRDKGRILTRNVM
                     GAIKEGDILMLLDTIREAKEIRTP"
     gene            complement(1615..1986)
                     /gene="rpl7ae"
                     /locus_tag="PKK85_04620"
     CDS             complement(1615..1986)
                     /gene="rpl7ae"
                     /locus_tag="PKK85_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HNS25335.1"
                     /translation="MAKAMYVKFDVPKELADKAYEALEIARDTGKITKGTNEVTKAME
                     RGEALLVLIAEDIDPPEIAAHMPVLAEEKEIPYVYIPTKDELGEAAGLNVGTASASIT
                     DAGEAAELIKDVAEKVEELKK"
     gene            complement(2323..2526)
                     /locus_tag="PKK85_04625"
     CDS             complement(2323..2526)
                     /locus_tag="PKK85_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HNS25336.1"
                     /translation="MELPIAPIGRIIKNAGAERVSDDAREALAKALEEKGEAIALDAV
                     KLAKHAGRKTVKAADIEMVVKKL"
BASE COUNT          677 a          649 c          595 g          870 t
ORIGIN      
        1 aggagcatgg ttaaaagttc agcgattccc tcaccagtct tggtactgat ggggatgatg
       61 cttacctgcc tggcgaagtc cctcacccgg tcgaagcgtt ccgattcaaa accctcctgg
      121 tggagaattc ccaccagttc gtagacctgg ttgtcaagag caacctgtac ctgggggttt
      181 tgtttgttgt aagtcttgtt gaatggttct ttttcgtgga tatgccagcc aggtatccga
      241 tctatcttgt tggcacccac cacgaagggg gtttggtaca ttttaaggat gttaagggcc
      301 tcgtaggtct ggggtttgaa gccctcgttg aggtccacca tcagcacagc caggtcagct
      361 agggctccac cccttttacg gagggtggtg aatgcttcat ggccaggggt gtcaatgaag
      421 aacaggccgg gcagactttc ccggatctcc agtttctgga tgaactggct gcagatggac
      481 tcaatggctt ccatgggaat ctcagtggct ccgatgtgct gggttatgcc cccggcttcc
      541 ttctgggcta tgaaactgcc ccttatataa tctaggagag tggtttttcc atggtccaca
      601 tggcccagta cagatacaat tggtgatctg atcttcaagt ttttcaaccc tcttactaaa
      661 aagcgccctt taaaaagtgt agagcaccaa aaaaatttaa ggccctaagt atttgtaaat
      721 taaggttctt cctgtatcca ttcccggtcc gggaaggaac aatcacatat ttcatccatc
      781 ttgaaaaaga gccctatttc cctttctgca gatgctggag aatcagaggc gtgtattatg
      841 tttcgaccag tgtcaatggc ataatcaccc ctgatggttc ctgtatcagc ttctttagga
      901 ttagtggccc ccaccatttt acggatgagg gtgatgcatt cttctccttc aatcactgtt
      961 gccaggactg gaccggaggt tatgtactcc accaggtcag tgaagaaagg tttttcctca
     1021 tgttcggcgt agtgttgctg ggccagttcc cgggggatga ccatcatctt ggcagccact
     1081 atctccagtc cgcgctcctc gaaacgggtc aggatcttac cagtcaatcg acgcagtact
     1141 gcatcgggtt ttaacataac gaagcttttt tccttcattg tttaacccac ttgacctttc
     1201 taggcactct tcccagtttt atacggtttt tttcacattt actgctgcag aaaaagaaga
     1261 ctgtaccgtc ttttttgacg aacatcttcc ctgtgccctc gtctatttct tctccgcaga
     1321 atgaacaagt tctcatagta acaccttaac ctctagggtg tgcgtatttc tttggcttcc
     1381 cggatggtgt ccaagagcat caggatgtcg ccttctttaa tggctcccat aacgttcctg
     1441 gtgagtattc gacccttatc ccttccgtcc agtatcctgc acttcacctg catgacttcc
     1501 ccagtcatgc cagttctctt taagacttct attacttcgg caggagttcc atcttccata
     1561 taatcacctt cgtaagaaga gaaaggtggt aacctctctc tagaaatagc ttaattattt
     1621 cttgagttct tccaccttct ctgctacatc tttgatgagt tcggcggctt cgccggcatc
     1681 agtaatgctt gcagatgcgg ttcccacatt tagcccggct gcttcaccca gttcatcctt
     1741 ggtagggatg tatacgtagg gtatttcctt ttcctcagcc agtactggca tgtgagctgc
     1801 tatttcaggt ggatcaatgt cttctgcgat taatacaagt agtgcttcgc ctctttccat
     1861 ggccttggtt acctcgttgg ttcccttagt aatcttaccc gtgtcccggg ctatctccag
     1921 ggcctcgtag gctttgtcag ctaattcttt tggtacatca aattttacat acattgcttt
     1981 tgccatttat ttacctcctt tttcatctgg tttcaaccat ccatcggcag atccaaactt
     2041 acacgcctca gggggtgtaa gagctcatgc cggatcagtt aaatgaccgt caatgatgaa
     2101 ttctaatttt gaaactcata aaaagcaatt cgaatctaaa attagatatc aaaaaattag
     2161 ttggaattgg tatatatact tatcattgga ccaggatgag tgccaccagg gatcatggtg
     2221 gactaagaaa tggtgccata aaaaaatgtc ctagtaggga aaagtaagaa aataggaaaa
     2281 taagtagcta aaaaaaagga taacaaaaag gtgtaaggtt acttacaact ttttgacgac
     2341 catttcaatg tcagctgctt taacagtttt acgaccggcg tgtttagcta gttttacagc
     2401 gtctaaggct attgcttcac ctttttcttc taatgctttg gccagagctt ctcttgcgtc
     2461 gtcagaaact ctttcggcac cggcgttctt tattattctt ccaatgggag ctattggtaa
     2521 ttccatatta ttcacctcaa ttttgtagat ttgttaatat acactgttgt tgtactctta
     2581 tatatattta tccctaattg cagtgattat tcggtcattc atatggtatg gggtcactac
     2641 atccagattt atatgatctt gggtcatcta gttaccaatc gtgacattaa agatagggtg
     2701 taaataatca gcatatccag atcaaccaga ttacccagaa gataaatata ggccaaaatc
     2761 gttcaaaccc tgcttataat cctggaaagg g
//
LOCUS       DAOQVW010000175         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43044_length_2778_cov_4.481087, whole genome shotgun sequence.
ACCESSION   DAOQVW010000175 DAOQVW010000000
VERSION     DAOQVW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43044_length_2778_cov_4.481087"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..737)
                     /locus_tag="PKK85_04630"
     CDS             complement(84..737)
                     /locus_tag="PKK85_04630"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNS25337.1"
                     /translation="MTSQEEMVENLWSSGYLHSRKVKEALLKVPREEFVPENKRSYAY
                     MDTPLPIGEGQTISAPHMVAILCEELALKEGMKILEIGTGYGYNAAVVAEIIGEKGHV
                     YTLERIESLAHRAMDNLKRTGYDKRVKVILTDGTQGYPEEAPYDRIYATASAPRVPEP
                     LKTQLIIGGKLLSPVGSDHYFQELVSVIRISEEEFQTKKLGGVAFVPMIGKHGWPEE"
     gene            complement(734..1333)
                     /locus_tag="PKK85_04635"
     CDS             complement(734..1333)
                     /locus_tag="PKK85_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HNS25338.1"
                     /translation="MKCPACGSCEHQILKSKGKLTKELLLKCEECGNVYRETMESPRP
                     MEVRIIISKFDESLKKWASFKPDEILEVGDVLEIGDERVRITSLENKRNARVMESMVE
                     DLISIWAVSVDIPARVGVSVDLHGRVLSRKVELDRDFTFKVGDVVKMGRTIFRIKTIK
                     TLERRMRRGFAYADVTKRVYGIPVSERRYDYDLTMNVVT"
     gene            complement(1416..1907)
                     /gene="hacB"
                     /locus_tag="PKK85_04640"
     CDS             complement(1416..1907)
                     /gene="hacB"
                     /locus_tag="PKK85_04640"
                     /EC_number="4.2.1.114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitase small subunit"
                     /protein_id="HNS25339.1"
                     /translation="MKILEGQVWTFRDHVDTDVIIPGRYLRILDLDEMSSHVMEGEDP
                     DFASQVKNGDIIVAGVNFGCGSSREQAPVALKHLGVAAVVAPSFARIFYRNAINVGLP
                     VIMADVEAEKGDILSIDLEAGIILNKSNGKKFEIQPFQNFMLEILEDGGLVNHFLKNR
                     GNS"
     gene            complement(1953..>2778)
                     /locus_tag="PKK85_04645"
     CDS             complement(1953..>2778)
                     /locus_tag="PKK85_04645"
                     /EC_number="2.4.2.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /protein_id="HNS25340.1"
                     /translation="PNRRKEYQDKIESASRKMMEFLGMEGDFDFLVGETYPPHSGLGT
                     GTQLSLAAGKLISNYLGQDIDAHSIGRIVGRGGTSGIGVAAFQQGGFIIDGGHLKSEK
                     SEFLPSSASQASPPPIIARYDFPDDWKILLVIPRFEDRVWGKKEVNIFQEYCPIPLQE
                     VEKLSHLLLMKMMPAVAEGDLDTFGEAVNSIQDMGFKKIENKLQNIVIGNIMESLRSA
                     GAAGAGMSSFGPTVYAITDTNPKGLINAVEDVLGSIDREIILTRAQNQGAVIKQDV"
BASE COUNT          727 a          680 c          513 g          858 t
ORIGIN      
        1 tataggtgca tccataggtt tctaaataga atttcatttc taatcaattt ctttattggt
       61 aaatggagag gaaataattc tcatcactct tcaggccagc catgtttacc aatcataggt
      121 acaaatgcca ctccacctaa ttttttggtc tggaactctt cttcggatat tcggattact
      181 gaaaccagtt cttggaagta atgatcagaa ccaaccgggc taagaagttt tccaccgatg
      241 attaactgag ttttcaatgg ttccggcaca cggggggcgc tggccgtggc ataaatgcgg
      301 tcatatggtg cttcttcagg gtaaccttgg gtcccatcag ttaagatcac cttcaccctt
      361 ttatcgtagc cagtgcgctt caaattatcc attgccctat gggccagtga ttcaatccgt
      421 tcgagggtgt aaacatgacc tttctcgcca attatttccg ctactactgc ggcgttataa
      481 ccgtatccag ttccgatctc taggattttc atcccctctt ttaaagcaag ttcttcacat
      541 aaaatagcca ccatatgagg ggcggatatg gtttgaccct cccctatggg taatggtgtg
      601 tccatgtaag catatgaacg cttattttca ggaacgaatt cctctcgggg caccttaagg
      661 agggcctcct tcaccttccg agaatgcaga tatccactgc tccacaaatt ttctaccatt
      721 tcctcttggg aagtcatgtt accacattca tggttaaatc ataatcgtaa cgtcgttccg
      781 ataccggtat tccatataca cgctttgtaa catcggcata tgcaaatcct cgtcgcatgc
      841 gcctttctaa agtttttatg gtttttattc gaaagatagt tctgcccatt ttcaccacgt
      901 cccctacctt gaaggtgaag tctcggtcca gttctacctt acgtgataaa actcttccat
      961 ggaggtccac ggaaacaccc acccgggccg gtatatctac tgatacagcc cagatgctta
     1021 tcagatcttc caccatactt tccattaccc tggcatttct tttattctcc agggaagtga
     1081 tgcggactct ctcatcccct atctccagta catcacccac ttccagaatt tcatcaggtt
     1141 taaaactggc ccattttttt agggattcat cgaacttact gataataatc cttacctcca
     1201 tgggccgtgg ggactccatg gtttccctgt atacatttcc acattcttca cactttaata
     1261 gtagttcctt ggttaattta ccctttgatt taagtatctg atgctcacag gagccacatg
     1321 caggacattt catcattttt ctccgtaaaa atagaattca tcacccaaaa aaatgtataa
     1381 ccttattgat atcatgtcat ttatctttta tttttttaac tatttcccct gttttttagg
     1441 aaatgattaa ccaggccgcc atcctcgaga atttccagca tgaagttctg gaagggctgt
     1501 atctcaaatt ttttcccgtt gcttttattt agtatgattc cagcttccag atcaatggat
     1561 agaatatctc ccttttcagc ttccacatcg gccatgataa cgggtagacc aacattaatg
     1621 gcattacgat aaaaaatgcg tgcaaaagag ggagccacca ctgctgccac ccctaaatgc
     1681 ttcaatgcaa ctggtgcttg ttcccttgat gatccgcaac cgaaatttac accagcaacg
     1741 atgatatcac cattttttac ttgactggca aaatcaggat cttcaccctc cataacatgg
     1801 gatgacattt catccagatc cagaatccga agataacggc cggggattat cacatcggtg
     1861 tcaacatgat ctctaaacgt ccaaacttgg ccttccagta ttttcatgat aaggttcctc
     1921 tataattaga ttctcttcac tattcatata acttatacat cttgttttat aactgcaccc
     1981 tgattctgtg ccctggtgag gattatttca cgatcaatgg accctagaac atcttccaca
     2041 gcatttataa gtcctttagg attagtatca gtaattgcgt atacagtggg gccaaaacta
     2101 ctcatacctg ccccagctgc ccctgctgac cggagcgatt ccataatatt gccaatgact
     2161 atgttttgta gcttgttttc gatcttttta aagcccatat cttgtataga gttcactgct
     2221 tcaccaaagg tgtccaggtc accctcggca actgcaggca tcattttcat cagaagcaga
     2281 tgtgaaagtt tctcaacctc ctgtaaaggg atgggacagt actcttgaaa gatgttaact
     2341 tctttttttc cccatactct atcttcaaag cgtgggatta caagcagtat tttccaatca
     2401 tcaggaaaat cgtaacgggc aattattggt ggtggggaag cttgagaagc cgatgaaggg
     2461 agaaattctg atttctcact tttaaggtgg ccaccatcaa ttatgaatcc accctgctgg
     2521 aaggcagcaa ctcctatacc agatgtacca ccccggccca ctattctgcc aattgaatgg
     2581 gcatcaatat cctgaccaag ataattagat attaatttcc cagctgcaag tgatagttgg
     2641 gttccagttc ccaagcctga atgaggagga taggtttccc ctaccaaaaa gtcgaaatca
     2701 ccttccatgc ccaggaattc catcatcttg cgggatgcgc tttcaatctt gtcctgatac
     2761 tctttgcggc ggttgggg
//
LOCUS       DAOQVW010000176         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43160_length_2774_cov_2.701361, whole genome shotgun sequence.
ACCESSION   DAOQVW010000176 DAOQVW010000000
VERSION     DAOQVW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43160_length_2774_cov_2.701361"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2774
                     /gene="polC"
                     /locus_tag="PKK85_04650"
     CDS             <1..>2774
                     /gene="polC"
                     /locus_tag="PKK85_04650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HNS25341.1"
                     /translation="KEDEFKVREQKADQAIRTALSILTEGVVAAPLEGIARVLIKRNF
                     DQTWYLAVYFTGPIRSAGGTASALAVLIADYIRISLGLDVYKPTEREIERYVEEVELY
                     ESEVTNLQYSPSPEEVRLAAQHIPVEVTGEPTDQVEVSHRDLERVETNHIRGGALLAL
                     AEGVIQKAPKVLKYAKKLHIDGWDWLGDFSKPQEKEGSEEDAPKVDKYLQDIIGGRPV
                     LAYPHANGGFRLRYGRSRNTGLAAMGIHPATMEIVEFLAVGTQMKIEKPGKGNCVVPC
                     DSIDGPLVKLRDGSVVNVTSVAQAKKIKHQVEEILFLGDMLVAFGEFLRNNHVLLPAG
                     WCEEWWIELLKIAPEFPEGSDDLLKEVQDGQISVEQAFELSLQYGIPLHPSYTYFYHD
                     LTREELNQLRGWLYQGVEGPVFDGDLELDLAPEKRVLEVLGVPHQVADHQVIIPRDHA
                     YSLINTLKQPLNLEDDLETLEALNTVSPVGIMAKAPTYLGGRMGRPEKSKERLMKPAP
                     HALFPIGTNGGSRRNIIQAAKKGTITVDIARCKCTECGVVSFQASCPVCGAPTITTGA
                     GNKRINISNTLKKAYQRVGVRKLDEIKGVQGMISETKFPEPLEKGVLRAKNNVFTFKD
                     ATIRHDSTNLPLTHFTPHEIKVSPQKLRELGYTHDTHGKELVHEDQVVEIFIQDLVIS
                     DDCANYLMRVANFVDDLLERFYHMEPHYHVKTRDDLVGHLAVGLAPHTSAGVLGRIIG
                     FTKASACYAHPYFHSAKRRNCDSDEDSVMLLLDALLNFSKSYLPNSRGGRMDAPLVLS
                     SRIDPEEIDDESHHLDTMDHLPLELFQKTLEECKPSEVLELVDNVKNRLGADEQYKGL
                     IYSHETGNIHRGPKICLYKVLPSMREKVESQIKLAEKIRAVDQKGVVEGVLNSHFLPD
                     MAGNSRAFSR"
BASE COUNT          747 a          647 c          758 g          622 t
ORIGIN      
        1 aaagaggatg aattcaaggt aagggagcaa aaggcggatc aggcaattag aactgcattg
       61 tccatactca ctgaaggtgt ggtggccgcg cccctagagg gcatagcccg ggtccttatc
      121 aaacgcaact tcgaccagac ctggtacctg gcggtgtact tcactggccc tatccgcagt
      181 gccggtggaa cagcatctgc cctggcagtg ctcatcgcgg attacataag aatttctctg
      241 ggtttggatg tttacaaacc tacggagcgc gaaattgagc gttacgtgga ggaagtggaa
      301 ctttacgaat cagaggtaac caacttgcag tactcaccca gccctgaaga ggttcggctg
      361 gcagcccagc acatcccagt ggaggttact ggggagccca ctgaccaggt ggaggtgtct
      421 caccgggatt tggagagggt ggaaaccaac cacatccgcg ggggagcgtt actggcccta
      481 gcagaggggg tgattcaaaa ggcccctaag gtgcttaaat acgctaaaaa gcttcacatc
      541 gatggatggg actggttggg tgatttttca aagccccagg aaaaggaagg tagcgaagaa
      601 gatgctccta aggtggataa gtatctgcag gatatcatag gtggaaggcc tgttttagca
      661 tatcctcacg ctaatggggg attcaggtta agatacggac gctctagaaa tacaggactg
      721 gcagccatgg gaatacaccc tgctaccatg gagatcgtag aattcctggc agtgggaacc
      781 cagatgaaaa tagaaaaacc gggtaaaggg aactgtgtgg tcccctgtga tagtattgac
      841 ggccccctgg ttaaactacg ggatggtagt gtggtcaatg taacatctgt tgcccaggcc
      901 aagaaaataa aacaccaagt tgaggaaata ctcttcctgg gtgatatgct ggtggctttt
      961 ggcgagttcc tccgcaacaa ccacgttctc ctgccggcag gttggtgtga ggagtggtgg
     1021 attgaacttc tgaagatcgc tcctgaattc ccggaaggct cagatgatct tctaaaggaa
     1081 gtacaggatg gtcagataag tgtagaacag gcttttgaac tttcccttca gtatgggata
     1141 cccttacacc catcttacac ctacttctac catgatctta cccgggaaga attgaaccaa
     1201 ctccgaggat ggctgtacca gggggtggag gggccagtat ttgatgggga tctagaattg
     1261 gacttggcac ccgagaagag ggtgctggaa gtgctgggag taccgcacca agtggctgat
     1321 catcaagtta taatccccag agaccatgcc tactctttaa taaacacttt aaagcaacct
     1381 ttaaatctgg aagatgactt agagacactg gaggctctga atacagtatc tccagtgggg
     1441 atcatggcca aagcacccac ctacctgggg gggaggatgg gaaggcctga aaagtccaag
     1501 gaaagattga tgaaacctgc ccctcatgcc ttgtttccaa taggcaccaa tggtggcagt
     1561 cggagaaata tcatccaagc cgccaagaag ggaaccatta ccgtggacat tgcccggtgt
     1621 aagtgcacag aatgtggtgt agtgtccttc caggcttcct gcccggtttg tggagcgccc
     1681 accatcacca ctggggcagg gaataagcgt ataaacatat ccaacaccct gaaaaaagcc
     1741 taccaaaggg tgggggtccg taaactggat gagattaagg gggtgcaggg aatgatctct
     1801 gagaccaagt tcccggaacc cctggaaaag ggggtgttac gggctaagaa taatgttttc
     1861 accttcaaag atgcgaccat tcgccatgat tctactaatc ttcccttaac tcatttcact
     1921 ccccatgaaa ttaaggtcag cccccaaaaa cttagggaac tgggttacac ccacgataca
     1981 catggaaagg aactggtgca tgaggaccag gtggtggaga tattcatcca ggatctggtg
     2041 atctctgatg actgtgccaa ctatctaatg agggtggcca actttgtgga tgatcttctg
     2101 gaacgttttt accacatgga accccactat catgttaaga cccgggacga cctggtaggg
     2161 cacctggcag ttggactggc accccacaca tcggcggggg tgctgggccg aatcatagga
     2221 ttcaccaaag cctcggcatg ttatgcccat ccttacttcc actctgccaa gaggaggaac
     2281 tgtgacagtg atgaagactc ggtgatgctc cttctggatg cccttctaaa cttctccaag
     2341 tcctacctcc ccaacagcag ggggggtcgt atggacgcgc cactggtttt atcctcccgg
     2401 atcgatccag aggagataga tgatgagagc caccacctgg acaccatgga ccatcttcct
     2461 ctggaacttt tccagaagac cctggaggag tgtaaaccat ctgaggtgtt ggaactggtt
     2521 gataatgtca agaatcgtct gggggctgat gaacagtaca agggactgat ttattctcat
     2581 gaaactggaa acatccaccg tggacccaag atctgcctct ataaagtctt accctccatg
     2641 agggagaagg tagaatcaca gattaaactg gctgaaaaga ttagggccgt tgatcagaag
     2701 ggtgtggtgg agggagtgtt aaactcccac ttccttccag acatggcagg aaacagcaga
     2761 gctttttccc gcca
//
LOCUS       DAOQVW010000177         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43317_length_2766_cov_3.273700, whole genome shotgun sequence.
ACCESSION   DAOQVW010000177 DAOQVW010000000
VERSION     DAOQVW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43317_length_2766_cov_3.273700"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PKK85_04655"
     CDS             <1..450
                     /locus_tag="PKK85_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNS25342.1"
                     /translation="IGKLRRFKSSQGLPLNTPLQGVAIYTTDEAVKNDLEASHQDIQG
                     TIRVGELGVKVGRPEVREVVVEVTPRMDRVGPHYKKDATRIQAYLESQDPQDLGEILE
                     RDGQLEVEELIITPDFLQTRKEVVGRTGEKVHLIQVEELGLVLEIQV"
     gene            507..1796
                     /gene="aroA"
                     /locus_tag="PKK85_04660"
     CDS             507..1796
                     /gene="aroA"
                     /locus_tag="PKK85_04660"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNS25343.1"
                     /translation="MEFQVEKTEKIQGVVQAPPSKSYTHRAFFLSALASGESTIISPL
                     YSEDTLASLVACQAFGSQFQVSDGQSLVEGTAGDLQTPGDVLDLKNSGTTLRLATTMA
                     SLAPGCTVLTGDESLRTRPMQPLLDALLKLGVKAWSTRGCGLAPIIITNGFQGGATSI
                     PGDVSSQYISSILLSAPYSQEGVELEVTGDFISQPYVEMTLDIMAHFRVKVDYHADDK
                     SFQVEPQTYQAQDYTVEGDYSSASYLVAAAAILESELTIRNLFQDSRQGDKVILDIVE
                     RMGVGVKHSSDEVKLEGNGEITGVEVNLEDTPDLLPTVAVLGALAEGTTTISGVEHAR
                     FKETDRISTTTQELKKMGFQVQEKRDGLILGGKIPESISGVVVDSHGDHRLAMALSLV
                     GLKLGRMRVQNAEVHSISFPRYREVMEKLGCQIKATP"
     gene            2332..>2766
                     /locus_tag="PKK85_04665"
     CDS             2332..>2766
                     /locus_tag="PKK85_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS25344.1"
                     /translation="MTSNRNPKLREKETKILILGSYNSGKTTTLENICHNKAKVEYNG
                     TTISLDYGNTQVKGKKVHLFASPGQERFRFMREVLSRGMDGAIVVVDNSQGVTSLDLE
                     IIQTLHQGKVPYVVFANKQDLNSAELDLNGFQAQVIPTTAHQG"
BASE COUNT          721 a          725 c          765 g          555 t
ORIGIN      
        1 attggaaagc tgcgccgctt caaatccagc cagggattac ccctcaacac acccctccag
       61 ggtgtggcca tttacaccac agacgaagca gtgaagaatg acctggaagc atcccaccag
      121 gatattcagg gcaccatacg ggtaggggag ttgggggtga aggtgggccg tccagaggtc
      181 cgggaagtgg tggtggaggt caccccccgc atggacaggg tgggacccca ctataagaag
      241 gatgccacca ggatccaggc ctacctggag tcccaggacc cccaggactt aggggagatc
      301 ctggaaaggg atggccagtt ggaagtggag gaattaatca tcacccccga cttcctgcag
      361 acccgtaaag aggtggtggg caggaccggt gagaaggtac acctcatcca ggtggaggaa
      421 ctgggcctgg ttctggagat ccaagtctag gataagttta gaataactac atggtgaaga
      481 attaatccgc tcattaggtg ttttaaatgg agttccaggt ggaaaaaact gaaaagatac
      541 agggagtggt gcaggcaccg ccctccaaga gttacaccca ccgtgccttc ttcctgtcag
      601 cactggccag tggagaatca accattatat ctccactcta ctctgaggat acactggcct
      661 ccctagtagc ctgccaggca tttgggagcc agttccaggt tagtgatgga cagtctctgg
      721 tggagggcac tgctggtgat cttcaaaccc cgggggatgt cttggatctt aaaaactccg
      781 gcaccaccct ccgcctggca actaccatgg cctccctggc tccagggtgt accgtactca
      841 ctggggatga gtcactccgc accaggccca tgcagcccct cctggatgca cttctgaagt
      901 tgggagtgaa ggcatggtcc acccggggtt gtggactcgc ccccatcatc atcacgaacg
      961 gtttccaggg tggtgctacc tccatacccg gtgatgtgag ctcccagtac atctcctcca
     1021 tcctcctatc cgcaccatac tcccaggaag gggtggaact ggaggtcacc ggtgatttca
     1081 tctcacaacc ctacgtggag atgaccctgg acataatggc ccacttcagg gtgaaggtag
     1141 actaccatgc tgatgataaa agtttccagg tggagcccca gacctaccag gcccaagact
     1201 acaccgtgga gggggattac tcctcggcat cctatctggt ggctgctgct gccatcctgg
     1261 agtcagagct aaccatccgt aatttgttcc aggattcccg gcagggagat aaggtcatcc
     1321 tggacatcgt ggagagaatg ggggtggggg tgaagcacag cagcgacgaa gtgaagttag
     1381 agggcaacgg tgagatcaca ggggtggagg ttaacctgga agacacccct gaccttctgc
     1441 caaccgtagc tgtgctagga gccctggcag agggcaccac caccatatct ggtgtggagc
     1501 acgcccgctt taaggaaacc gaccggatca gcaccaccac ccaggaactt aaaaagatgg
     1561 gattccaggt gcaggaaaaa cgagacggac tcatcctagg gggaaaaatt ccggaaagca
     1621 tctctggggt ggtggtggac agccacggtg atcaccgcct ggccatggcc ctctccctgg
     1681 tggggcttaa actaggcagg atgcgggtgc aaaatgccga agtccacagc atatccttcc
     1741 cccgttacag ggaggtaatg gagaagttgg gctgccaaat caaagccact ccctagggga
     1801 tggaggaact tatataaaca ggagagagtt aaggttttcg tgatgtctga ccaggagaga
     1861 gtgatctgcc agaatgtaaa aagctgcgat cagagtaact gccagcacta ccagtggcat
     1921 gataaaacca gccaatgcag actccgacaa tgccatataa agaaaatcat gacctcctgt
     1981 gattcaacta tcccctgact gaagtgggtt accagtacaa gagcacgtac catgggtgga
     2041 aaagaacccc caattcttta ttctatgccc tagatcacgg tttgcttctt tcatatcgac
     2101 cttgtggggc ctattccatg atggtggagg ccctgtaatc ctgggtatca gatagattct
     2161 aggacgatcc aggccccccc aaactaaggt tctgctagga tgactccagg gaaggccatg
     2221 gccaagatta ggggagtggt gttacagatg gcaaggcctt atatatttgt tgaatacaaa
     2281 gtcatatcat aaagagctta tatattaatc caaccggtgc aggcggatac catgaccagt
     2341 aacagaaacc ccaagttacg tgagaaggaa accaagatat tgatcctggg atcttacaac
     2401 tcaggaaaga ccaccaccct ggagaacatc tgccataaca aggccaaggt ggagtacaac
     2461 ggcaccacca tatccctgga ttatggtaac acccaggtca agggtaagaa ggtgcaccta
     2521 ttcgcctctc caggccagga acgcttccgt ttcatgaggg aagtcctgtc ccgggggatg
     2581 gatggggcca tcgtggtggt ggacaacagc cagggagtca ccagccttga cctggagatc
     2641 atccaaaccc tccaccaggg aaaggtgccc tacgtggtct tcgccaacaa acaggacctg
     2701 aacagtgctg aactggacct gaacggcttc caagcccagg tcatacccac cactgcccat
     2761 cagggt
//
LOCUS       DAOQVW010000178         2761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43434_length_2761_cov_3.572432, whole genome shotgun sequence.
ACCESSION   DAOQVW010000178 DAOQVW010000000
VERSION     DAOQVW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43434_length_2761_cov_3.572432"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..373
                     /locus_tag="PKK85_04670"
     CDS             155..373
                     /locus_tag="PKK85_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25345.1"
                     /translation="MKKKQIQECKEALQELVGKKVIDIRFEVQGREKDCWRFYIDTDD
                     GVFTMTFCREWECPVVSHSPEHFISNPP"
     gene            complement(360..1649)
                     /locus_tag="PKK85_04675"
     CDS             complement(360..1649)
                     /locus_tag="PKK85_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNS25346.1"
                     /translation="MIGISADFDPVHRGHVKLIQKAREVADEKGSEVVIYLNKGYSAN
                     HAPFFVGYDARRRMALAAGADRVVPIEGLHHRLTMAYTVPIRIAMMIQDGVRDYVDAT
                     DVSPNQIKKYASDFVKSGIFSGIPRNLPNRNVIRWYAVNEFLGKVLGRRMKFHFIPEL
                     KVQDEKISGRIIRRKILENNLRIPEGVRKLLPTSTITILQEEIDQGNIPGERNLEVLL
                     DRLNNYPRPGLLEIAHLNAAAVDEIIEGRKYRKETPAWASLRKAGYGPVLTRLALSCV
                     EENVTRREVFDLIKSYQKERIIPPDQTVERVIGRAWFVASSVEKGMDSHEAHERFRKG
                     EIIRHKVPYTVNAGIHLRSFELSSLQEGLPAQLYVDKRGMISCLIKTRERKIKSPLKL
                     QARDATYLRLLLDSQIIPLQGSMISKKKGWRVQIKVG"
     gene            complement(1859..>2761)
                     /locus_tag="PKK85_04680"
     CDS             complement(1859..>2761)
                     /locus_tag="PKK85_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25347.1"
                     /translation="WIKVSGMLQHLTIACAIVYALTIMEGVITLQPGIIITPQIAVIT
                     IIYGLSFFYLSWCLHKVSHIYPSNDMGNPEINTQDKNGIRSKGIFKLFREASLSLTFA
                     VLILMGVLLTLLGILLFPVNSGLIPFSPDGQFGLLLFIFSIQMMALGETLLGPFKRSW
                     LMVIIGLAFAVLGIFSCIVPGILTVVIPKLIGSLSIIGGALLITQRFLSQQQEKIHEN
                     MKPDLEKTEIESINYKLMITQTLINIVAIVFGISALMEGLLPGMIVAGLLVTYGLILF
                     VLVFILHKMTLIEIKGKDLSEAAV"
BASE COUNT          747 a          603 c          597 g          814 t
ORIGIN      
        1 tgcaccaagt cttccaaaaa ctggataaaa aagagtaggg ggaataattc ttgggagaag
       61 cccccttcat cagggatgta cttggccctc acttaattat attacactac aatgacataa
      121 tattagatat acattgtttg ttgtggtggc ttcgatgaaa aaaaagcaga tacaggaatg
      181 taaggaggcc ctccaggagt tggtgggtaa aaaggtcatt gatatccgct tcgaagtcca
      241 gggcagggaa aaggactgct ggaggtttta catagacact gatgatggtg tgtttaccat
      301 gaccttctgc cgggagtggg agtgtccggt ggtaagtcac agccctgaac acttcatctc
      361 taacccacct tgatttgcac tctccagcct ttctttttac tgatcatgct gccctggagt
      421 ggaatgatct gggaatctaa aagcagccgg aggtaggtgg catcccgtgc ctgcagcttc
      481 aatggacttt taattttacg ttcacgagtt ttaatcagac aggatatcat tcccctctta
      541 tctacgtata actgggcagg aagtccctcc tgcagggagg ataattcaaa actgcgaaga
      601 tggataccgg cgttaacagt gtaggggact ttgtgacgaa ttatttctcc cttccgaaat
      661 ctttcatgtg cttcatggct gtccatcccc ttttcaactg atgaagccac gaaccaggcc
      721 cggccgataa ctctttccac agtctgatca ggaggtatga ttctctcctt ctggtaactt
      781 tttataaggt caaagacctc ccggcgggtg acattctcct ccacacaact cagggccagc
      841 ctggtgagaa ccggcccata gcctgctttt ctaagagatg cccaagctgg agtttcttta
      901 cgatatttcc tcccctctat gatttcatcc acggcggctg catttaagtg agcgatttct
      961 agaaggcccg ggcgggggta gttatttaaa cggtccagaa ggacttccag gtttctttca
     1021 ccaggaatgt taccctggtc aatctcttct tgcaagatag ttatagtgga agtagggagc
     1081 agcttgcgaa caccttctgg tattcttaag ttgttttcca ggatttttct gcggatgatg
     1141 cgccctgaga tcttctcatc ttgcaccttt aattcaggga tgaagtgaaa cttcatcctt
     1201 ctccccagaa ccttccctag aaattcgtta actgcgtacc atcgtataac atttcggttg
     1261 ggcaagttac gtgggatgcc actgaatatg ccacttttca caaaatccga ggcgtacttc
     1321 ttaatctggt tgggtgatac atctgtagca tccacgtaat ccctcactcc gtcctggatc
     1381 atcatggcaa tccttatggg cacggtgtaa gccatggtca agcggtgatg caagccttca
     1441 atggggacca cccggtcagc cccggctgct aaggccattc tcctccgggc atcgtaaccc
     1501 acgaagaaag gggcgtggtt ggcactgtaa cctttgttga ggtagataac cacttcgctg
     1561 cccttttcat cggctacttc cctggctttt tggatgagtt tcacatggcc cctgtgtacg
     1621 gggtcaaagt ctgcgcttat tcctatcact tgtcattcac tctattttcc aactaaaagt
     1681 cccaataata ctaataaagc catattattg ttttttgccc ttaatataaa ttttattctt
     1741 taaaaataat tgtctgatgg ctttaatatt attttgaaga acagttacca tatttttctg
     1801 gatgtttcgt ctgattggtt tatgggtaca tatttatggt gaagtttttg gttttggatt
     1861 atacggcagc ttcactaaga tcttttcctt tgatctcaat taaggtcatt ttgtggagga
     1921 taaatacaag cacaaagagg atgagtccgt aggtcaccag caatcccgcc acaatcatcc
     1981 cgggaaggag tccttccatt agagcagata ttccgaaaac aatggccacg atatttatta
     2041 aagtctgagt aatcatcagt ttgtaattaa tagattcaat ttcagttttt tcaagatcag
     2101 gtttcatatt ttcatgtatt ttttcctgtt gttgtgataa aaatctttgc gtaatcaaca
     2161 gggcccctcc aatgatactc aaagatccaa tgagtttcgg gataactacc gttaaaattc
     2221 cagggactat acatgagaaa attcccaaca cagcaaatgc cagtccaatt attaccatta
     2281 gccaggaccg cttaaagggg cctaaaaggg tttctcctaa tgccatcatc tggatggaga
     2341 agatgaacaa aagcagtccg aactgaccat caggggaaaa aggtatcaga ccactgttca
     2401 ctggaaaaag gaggattcca aggagcgtca gaagcacacc catcaagatt aatactgcaa
     2461 atgtaaggga tagggatgcc tccctaaaaa gtttgaaaat ccctttggaa cggataccat
     2521 ttttgtcctg tgtatttatt tcaggatttc ccatgtcatt tgaaggataa atgtggctaa
     2581 ctttatgaag acaccaggat agatagaaaa aacttaatcc atagataata gtaattactg
     2641 ctatctgtgg agtgatgatg atgccgggtt gtagtgttat cacaccttcc ataattgtca
     2701 aggcatagac tatagcacaa gcaattgtca agtgttgtaa catgcctgaa acctttatcc
     2761 a
//
LOCUS       DAOQVW010000179         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43557_length_2756_cov_3.620881, whole genome shotgun sequence.
ACCESSION   DAOQVW010000179 DAOQVW010000000
VERSION     DAOQVW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43557_length_2756_cov_3.620881"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKK85_04685"
     CDS             <1..1096
                     /locus_tag="PKK85_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNS25348.1"
                     /translation="HAEKVGTVVLHHVHPESLMQVDPKPGMSVKGMVDWDRRLALARN
                     HTATHLIVAAARQILGDHIWQAGAQKGIKKSRIDLSHYKRINAEELQWIELLANQWVM
                     ENWEVKTTWMERTEAEKTYGFILYQGGVVPGSRIRVVQIPGIDVQACGGTHVNQTGDI
                     GLVKINRTERIQDGVERLEFSAGSAAVEALQESEAQLRDSAAVFKVTPDQLPTTAERF
                     FTEWKAFRNEIKRLQGQVAALKKEKMAKNSEKIGGLNLIRDTIDAEMVELVKIVTELV
                     EKEDVDIAVLGNPEGKIVGAVSPKALEHGIKINEIIKGAAGVLGGGGGGKPQLAQGAG
                     RHPEKINEALKFVYNDIKSRLSQKSLNGFI"
     gene            1171..2424
                     /locus_tag="PKK85_04690"
     CDS             1171..2424
                     /locus_tag="PKK85_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 16 metalloprotein"
                     /protein_id="HNS25349.1"
                     /translation="MKRTIQEIEAKIENGEATVLTAEEITKMALNGEKLSTTDVDVIT
                     TGTCGVMSGTMGIFHLPVAEPGSFKKARHILLNGIPGIPGPCPNEWLGSVDLIVYGTA
                     YSLNDPDYGGGFLFKDLVKGKEIEVEVESSEGNVLRTPATLEDFGTAQLLGTRFAFKN
                     YTAFVNPTSQPISSIFNAVDMLGPLKGLSFSGCGELNPLQNDPELNTLKKGAKVLLNS
                     SEGIFIGQGTRSTPSKPNMMLTADMREMDSHYLGGYLTGAGPEVYNSVATAIPILDES
                     ILKRTFVKHEDIKLPIADIRGRHSVLSETDYGAVWNDVDERPQYREEDCRRCQECLVE
                     ERCPTGAFKNNYLNMQQCFGCGMCSFACPYQIFRMNTGQVKMDCDGEVMEIPVTCRQS
                     DLKRARELAEELKNRIIKGEFSLRW"
     gene            complement(2442..>2756)
                     /locus_tag="PKK85_04695"
     CDS             complement(2442..>2756)
                     /locus_tag="PKK85_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25350.1"
                     /translation="RERKRKPIPDKGAYKPLQNKFMRGEYQEHQEEQYPDDHYYNPQE
                     AYDYGDYPDEFYGEQAEVDGGKNSIWGTLILFMVLALVVGFVVGLLIFTSGSQNILSI
                     GV"
BASE COUNT          818 a          576 c          685 g          677 t
ORIGIN      
        1 acacgctgaa aaggtaggta cggtggtact gcatcatgtg catcctgaaa gtctcatgca
       61 agttgatcct aaaccaggga tgagtgttaa aggtatggta gactgggacc gtcgcctggc
      121 cctggcccgt aaccacaccg ccacccacct aatagtggct gctgcccgac agatactggg
      181 agaccatatc tggcaagctg gggcccagaa gggaataaaa aaatcccgaa tcgacctttc
      241 ccactacaaa cggataaatg ccgaagagtt gcagtggatc gaacttctgg ccaaccagtg
      301 ggtaatggaa aactgggagg ttaaaactac ttggatggag cgcaccgaag cggaaaagac
      361 ttatggcttt atcctgtacc aagggggcgt ggttccaggt tctaggataa gggtggtgca
      421 gattcccggg atcgacgtgc aggcttgtgg cggaactcat gtgaatcaaa ctggagacat
      481 tggcttggtc aagatcaacc ggaccgaacg catacaggac ggagttgaac gtctcgagtt
      541 ttcagcaggc agcgccgctg ttgaagccct gcaggagagt gaagcccaac ttcgggacag
      601 cgctgcggtg tttaaggtga ctcctgatca gctccccaca actgcagaac ggtttttcac
      661 cgagtggaaa gcctttagga atgaaataaa acgtctgcag ggacaggtag ctgctcttaa
      721 aaaggaaaaa atggcaaaaa attccgaaaa aatcggtgga cttaatttga tccgggacac
      781 catcgacgca gaaatggtgg aattagtcaa aatcgtcact gagttagttg aaaaagaaga
      841 tgtagatata gctgttctcg gaaatccgga aggcaaaata gtgggagccg tttctccaaa
      901 agccctggag cacggtatta aaattaatga aatcattaag ggagccgctg gtgtgttagg
      961 cggaggtggt ggaggtaagc cacaactagc ccagggagcc ggacgtcatc ctgaaaagat
     1021 aaatgaagct ctgaaatttg tttacaatga tataaagagc agattatccc aaaaaagcct
     1081 taatgggttt atttaaccca tacttctggt ataggctttt tttagatagt cagtgtagtt
     1141 tagtgggcat ggataaaaga gggggctaat atgaaacgca ccatccaaga aattgaggcc
     1201 aaaatagaaa atggtgaagc tacggtctta actgccgaag aaattacaaa aatggcctta
     1261 aatggggaaa aattaagcac aactgatgtg gatgtgataa ccaccggcac ttgtggtgtt
     1321 atgtccggaa ctatgggcat tttccatctg cccgtggccg agccaggatc atttaagaag
     1381 gcccgacaca tcctattaaa tggtatacct ggaattcccg ggccttgtcc caatgaatgg
     1441 ttgggatcag ttgatctgat cgtttatgga acggcatata gcttaaatga tcccgattat
     1501 ggcggaggat ttcttttcaa ggatctggtg aagggtaaag agattgaagt tgaggtagaa
     1561 tcatccgaag gtaacgtgct aagaactcca gctaccctgg aagattttgg tactgctcaa
     1621 ttattgggaa caagatttgc cttcaaaaac tacactgcct ttgtaaaccc tacatcccaa
     1681 cctatttcat ccatatttaa cgcagtggac atgttaggac cacttaaagg actttctttt
     1741 tctggttgtg gggaattaaa cccactacag aatgatccag aactgaatac cctaaaaaaa
     1801 ggtgctaaag tacttctcaa cagttctgag ggtattttta tcggacaggg caccagaagc
     1861 acaccaagca aaccaaacat gatgctaact gctgatatgc gcgaaatgga ctcccactat
     1921 ctgggtggtt atctcactgg tgctggtccc gaagtctaca acagcgtagc cacggccatt
     1981 cccatcctgg atgaatccat actaaaaaga accttcgtga aacatgaaga tatcaaactt
     2041 cccattgccg atataagagg aagacacagt gttttatctg aaactgatta cggggcggtg
     2101 tggaatgatg ttgatgaaag gccccagtac agggaagagg attgccgacg ctgtcaggag
     2161 tgtctggtgg aagaacgatg cccaactgga gcatttaaaa ataattacct gaacatgcaa
     2221 caatgtttcg ggtgcggcat gtgcagtttt gcctgtccct atcagatctt tcgcatgaac
     2281 accggacaag tgaaaatgga ctgtgatggt gaagtaatgg agataccagt gacctgccgc
     2341 caatcagacc ttaaacgtgc cagagaactg gcagaagaac ttaaaaacag gattattaag
     2401 ggagaattta gtcttaggtg gtagtctatg gttaatactt tttagacccc tatagataga
     2461 atattctggg agccactggt gaatatcagt aaacccacca caaatcccac aaccagagcc
     2521 aggaccatga acagaattaa ggttccccag atggaatttt ttcccccatc cacttcggct
     2581 tgttctccat aaaattcgtc aggatagtca ccataatcgt aggcttcttg tggattgtaa
     2641 tagtgatcat caggatattg ttcttcctgg tgttcctgat actctccccg cataaatttg
     2701 ttctggaggg gtttataagc ccctttatcc gggatgggct ttcttttcct ttcccg
//
LOCUS       DAOQVW010000180         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43565_length_2756_cov_3.177712, whole genome shotgun sequence.
ACCESSION   DAOQVW010000180 DAOQVW010000000
VERSION     DAOQVW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43565_length_2756_cov_3.177712"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1347)
                     /locus_tag="PKK85_04700"
     CDS             complement(1..1347)
                     /locus_tag="PKK85_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNS25351.1"
                     /translation="MKWTDPVKRIGDLILLLLNFLARIVIIVSKRSLKILYTPSVLFG
                     KVVHFFRDVSRTLGEGFVALFICAIGDLVAGLFLSSMTETLTILPGLLILIPGAIGMR
                     GNIFGALGSRLGSNLHIGILSPELKKSTILSQNIISAMILTLIMSIFLAFMAKGFSMF
                     WGYESISLVDFTIISVLGGIFSGALLLPATILITIKSYENGWDPDNVTTPLIAASGDL
                     FTLPSILLAVQILLWIRNGFLDTILFMAFMAIGIVAFMVGIRGEGNLKKIIKHSTPTL
                     FLSSILGASAGTILNSTFSVILNNPSILALIPLFSGESGDLVSILGARLSSGLHIGLI
                     KPSLRPSEEAVRNFGIIIILAIIIYPTIGLLAYLASTILGLETVGIENMVVISTLAGL
                     LLTPFMLMIAFYLSIVTYKQGLDPDNVVIPLTTSMTDPVANTFLVLMVISIIGYTL"
     gene            complement(1454..1891)
                     /locus_tag="PKK85_04705"
     CDS             complement(1454..1891)
                     /locus_tag="PKK85_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNS25352.1"
                     /translation="MDDIDLAILRSLIKNSRITLSQMSKEIDVPDATISNRLKKLESQ
                     IIHRYTIILDWQKTGLEITSIIIIQTESDKHGFVKEMLSRLEEVSEVYSVSGEYDILI
                     KVWVKSIEELNDLIDTKIRSIDGVEDLTEMIVLERVKDDIPAL"
     gene            complement(2140..2745)
                     /locus_tag="PKK85_04710"
     CDS             complement(2140..2745)
                     /locus_tag="PKK85_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNS25353.1"
                     /translation="MPKNIKDILIEMKNMSELMVDLAYSALLFNSKDAAEEVKKLENK
                     INRLNYEIKKESLLAARTVEDAEKLTALLEVGEATENIADSAKDIADLVLKGIKPHPV
                     FKMVMEESDEMIIRVKICEPSELAYNSLGELLLATRTGMRVIAIRRNESWIYGPDKNT
                     ILNANDTLIAKGNETGANILVKLASGEIKLEDLEYDEIAEK"
BASE COUNT          819 a          554 c          533 g          850 t
ORIGIN      
        1 ctataatgta tagcctatta tggaaatgac cattaggacc aggaatgtgt ttgcaacagg
       61 atctgtcata cttgttgtca gtgggatgac tacgttatcc ggatcaagac cctgtttata
      121 ggttacgatg ctaagataaa aggcgatcat caacatgaat ggcgtgagga gcaacccagc
      181 tagggtgctt attactacca tgttttcaat gcccactgtt tccagcccca aaatggtaga
      241 agcaaggtag gctaacaagc ctatggttgg atagattatg atggcgagga tgataattat
      301 cccaaaattt cttacagctt cttctgaggg ccggagagaa ggtttaatta atccaatatg
      361 taatccggaa gataatctag cacccagtat actcaccaag tcgccgcttt caccagagaa
      421 caaaggtatc agagcaagga tgctgggatt gtttaaaata accgaaaaag tgctgttcag
      481 gatggtacca gcagaggcac ccaggattga ggataaaaac agggtgggtg tgctgtgttt
      541 tatgatcttt tttaggttac cttcaccccg gatcccaacc atgaatgcaa caataccgat
      601 ggccatgaat gccatgaata atatggtgtc taaaaatcca tttctaatcc ataagagtat
      661 ttgtaccgcc agtaagatgg acggaagggt gaaaagatct cctgatgctg ctattaatgg
      721 cgtggttacg ttatccgggt cccaaccatt ctcataactt ttaatggtga ttaaaatggt
      781 ggctggtaag agcagtgccc cggagaatat tccccccaac acagatataa tggtaaaatc
      841 caccaagcta atactttcat agccccaaaa catggaaaaa cccttggcca taaatgccag
      901 gaaaattgac attattaagg ttaatatcat ggctgagatg atattctgag ataatatcgt
      961 tgattttttt agttcaggtg atagaattcc aatatgaaga ttggagccta accttgatcc
     1021 taatgcacca aaaatatttc ccctcatccc tattgcacca gggattagga taagtagccc
     1081 cggaagaatg gtcagtgttt ctgtcatgct acttaaaaaa agacccgcaa ctaggtcacc
     1141 gatggcgcaa ataaaaaggg caacaaatcc ttcacctaag gttcttgaaa cgtcacggaa
     1201 aaaatgaaca actttaccga aaaggacaga gggtgtgtaa aggatcttca gagacctttt
     1261 tgaaactatg ataacaattc tggctaggaa gttcaataaa agtagtatta agtctccaat
     1321 cctcttcact ggatccgtcc atttcaccta accacctgaa catggtgcct gctgatttgc
     1381 agtgcaccct aattaaattg cctaaatagc tgttgccatc gattttaatc atcgccatat
     1441 ccatcttatt cctttaaagt gccggaatat catctttaac acgttccaag acaatcattt
     1501 cagttaaatc ctcaacgcca tcaattgaac gaattttagt atctattagg tcgtttaatt
     1561 cctctatact cttcacccat actttaatga gtatatcgta ttcccctgat acgctgtaaa
     1621 cttctgatac ttcttccaat cgggataaca tctctttaac aaatccatgt ttatcagatt
     1681 cagtttggat gattattatg gaagttattt ccagaccagt tttctgccag tccaggatta
     1741 tggtatagcg atggattatt tgggattcca atttcttgag gcgattggat atggtggcat
     1801 caggtacatc aatttccttc gacatctgag aaagtgtaat ccttgaattc ttaatcaagg
     1861 aacgcagtat agccagatca atatcgtcca tttcaatcaa catttaggga gtgagtagta
     1921 ataggtgatg tccccttata aaggttggga atattactaa tttagtatga aaaattgagt
     1981 atttttctta tttttattca tttaattaga aagttcagct aaatttttag aattttcggg
     2041 aaagatcggg aaaggtgcaa agagggtgta ccgggtgcgg gagaaataat aattaggaaa
     2101 aaaatcagcc attcaaggga gtagctcaga taatatgaac tatttttcag caatttcatc
     2161 atattcaaga tcttcaagtt taatttcccc actggcaagt ttgaccagta tatttgcacc
     2221 tgtttcattt cccttggcaa ttaaggtgtc gttggcatta agaatagtgt ttttatccgg
     2281 gccataaatc caggattcat ttctcctgat ggcaataacc ctcatccccg tcctggtggc
     2341 tagtaaaagt tctcctaagg aattataagc caactctgat ggttcacata ttttaaccct
     2401 gatgatcatc tcatccgatt cttccatgac cattttaaat acgggatgcg gttttattcc
     2461 tttcaatacc agatcagcta tgtctttagc ggaatctgcg atgttttctg tggcctctcc
     2521 cacttccagt agggcagtca atttctccgc atcttccacg gttcttgcag ccaggagaga
     2581 ttctttctta atctcatagt tgagcctgtt tattttattt tcaagttttt taacttcctc
     2641 tgccgcgtct ttactgttaa aaagtagggc tgagtatgct aaatccacca ttaactctga
     2701 catgttcttc atttcaatta aaatatcctt gatatttttt ggcacctttc atcctc
//
LOCUS       DAOQVW010000181         2754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43606_length_2754_cov_3.128196, whole genome shotgun sequence.
ACCESSION   DAOQVW010000181 DAOQVW010000000
VERSION     DAOQVW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43606_length_2754_cov_3.128196"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKK85_04715"
     CDS             <1..337
                     /locus_tag="PKK85_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNS25354.1"
                     /translation="SDHALIIPCDSPCISPHFVEEMLEHPLEDYQALVPRWSDGRTEP
                     LHAVYSKEDVLPVIEKIIQQGRRDVQSIFNQLKVLYLDAESLDPGGNTFLNLNRLQDV
                     EVLEKKKLK"
     gene            complement(482..727)
                     /locus_tag="PKK85_04720"
     CDS             complement(482..727)
                     /locus_tag="PKK85_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudomurein-binding repeat-containing protein"
                     /protein_id="HNS25355.1"
                     /translation="MESLTPEQYRQMVKKVIDFKRLNGKLPESTVIDGCRIIKKDYID
                     MIEMVNKFYLETGRNPGLVEIGSGDQHCNNRENLLIH"
     gene            complement(856..1626)
                     /locus_tag="PKK85_04725"
     CDS             complement(856..1626)
                     /locus_tag="PKK85_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25356.1"
                     /translation="MKIGENLSWRVKLGIILVFTSIVLYIALYWTFHDPHQELFLLGI
                     DLAFVPLEVLFVVLVIETAIGEREKRIMMEKMNMVIGTFFSEVGAELLTRITNYDPDT
                     DKIRHSLLVNANWSAADYKRAIGDIEKFDYTLEITGRDANSLEFLVSTKEFLVQNRKF
                     LLGLMENPNLLEHETFTDLLRSVFHLTEELEGRANLTNLSKADYAHLAGDIIRVYSIL
                     IYEWICYMQHLMENYPYLFSLAVRKNPFDPEASVEIGG"
     gene            complement(1648..2214)
                     /gene="cbiT"
                     /locus_tag="PKK85_04730"
     CDS             complement(1648..2214)
                     /gene="cbiT"
                     /locus_tag="PKK85_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HNS25357.1"
                     /translation="MIPDEDFYKVPEVPGPSKEEVRCLVMCHSRVKEGDVVLEVGCGT
                     GGLTVELSRRAAKVYSVDKNPQALEVTLTNLRKLNPQGEVELILGDALEAMEDLPPYD
                     LLVVGGSSGELEEIIRKAYQQLQAGGRVVVTAILLETRLRSLKVMEDLEMEPRIVEVF
                     VARGRTTQMGTMMQGLNPVAIISGVKKL"
     gene            complement(2211..2732)
                     /locus_tag="PKK85_04735"
     CDS             complement(2211..2732)
                     /locus_tag="PKK85_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNS25358.1"
                     /translation="MARQLLEALKEAGKKTALLVTEPARIIIDYELEISASELESLAY
                     RVYDSKDLTAAINSGSCRFESMVIVPATMKTVAAISQGYASNAVTRTADVALKEHRKL
                     VMVPRETPLRQVHLENMLKLSREGVIILPAMPAFYHQPEDMGDLVDFVVGKILDVLCI
                     DHHLYQRWREGEP"
BASE COUNT          665 a          740 c          648 g          701 t
ORIGIN      
        1 gtcggaccat gccctgatca ttccctgtga ctcgccctgc atcagccccc acttcgtgga
       61 agaaatgctg gaacaccccc tggaggacta ccaggccctg gtacccagat ggagtgacgg
      121 ccggaccgag ccattgcacg ccgtgtacag taaagaggat gtcctaccag ttatagaaaa
      181 aataatccag cagggaaggc gggatgttca atccattttt aaccagttga aggtactgta
      241 cctggatgca gagtccctgg atcccggggg taataccttc ctcaacctca accgcctcca
      301 agatgtggag gtcttggaaa aaaagaagtt aaaatagtag gatccacgaa taaagctgcc
      361 attcgtgtac catagaaaag tgcctcactc attaagagcg agtacccaag ttaaagtaaa
      421 ataagcgtca attttttatt ccagggagtt taaggaacat ccgaagagtt gcgatgtttt
      481 tctagtgaat taagaggttt tctctattgt tgcagtgctg gtctcctgat ccgatttcca
      541 ccagaccagg gtttcttccc gtctccaagt aaaacttgtt caccatttcg atcatgtcga
      601 tgtagtcttt cttgataata cggcagccat ctatgactgt ggactctggg agttttccat
      661 ttaaacgttt aaaatctatt acctttttta ccatctgtcg atattgctca ggagttaagc
      721 tctccattac atcaaccaca ttattgtttt aggaatatat tagtcccacc ctttaaatag
      781 gttacgaaac atgagcatca gttcacaaag gaggaattct gggatgattt acaagccaca
      841 catccacaag aaaattcaac ccccgatctc cacgctggcc tcggggtcga agggattctt
      901 ccgcacagcc aaggaaaata ggtaaggata gttctccatt aaatgctgca tataacagat
      961 ccattcatat attaagatgc tgtagacccg tataatatca ccagcaaggt gtgcgtagtc
     1021 tgccttggag aggttggtta aatttgccct gccttccagc tcctcggtga ggtggaagac
     1081 ggagcgcaaa agatcagtga aggtttcgtg ttccaggagg ttggggttct ccatgaggcc
     1141 cagaagaaac ttcctgtttt gcactaggaa ctccttggtt gacaccagaa actccaggga
     1201 gttggcatcc ctgcctgtaa tttccagggt gtagtcaaat ttttcgatat ccccaatggc
     1261 ccttttatag tcggcagcag accagttggc gttaaccagg agggagtggc gtatcttatc
     1321 ggtgtccggg tcatagttgg tgatcctggt gagtagctct gcacccacct cactgaagaa
     1381 ggtgcctatg accatgttca tcttctccat cataatcctc ttctcccgct ccccaatggc
     1441 agtttcaatg accagtacca caaacaacac ctccaggggt acgaaggcca ggtcaatgcc
     1501 caggaggaaa agttcttgat gggggtcgtg gaatgtccag tacagtgcaa tgtaaagtac
     1561 tattgatgtg aacactaaga tgatgcccag tttcactctc caactcaggt tttcccctat
     1621 cttcatatga taagctccat tcctggttca gagttttttc acaccagata tgatggccac
     1681 ggggttcagt ccctgcatca tggtgcccat ctgggtggtt ctgccccggg ccacgaagac
     1741 ctccactata cggggctcca tctccaggtc ctccataact ttaaggctgc gcagtcttgt
     1801 ctctaggagt atggcggtga ctacaacccg gcccccagcc tggagttgct ggtaagcctt
     1861 cctgattatc tcctccaatt caccactact accccccacc accaggaggt cgtagggggg
     1921 caaatcctcc atggcctcca gtgcatctcc cagtatgagt tccacctccc cctgggggtt
     1981 cagtttcctg aggttggtca gggttacctc cagggcctgg gggttcttgt ccacagagta
     2041 gaccttggcc gcccgtctgg ataactccac tgtaagccct cctgtaccgc agcccacctc
     2101 caggaccacg tccccctcct tcacccttga atggcacatt accaggcagc ggacctcctc
     2161 cttactaggc cccggtactt caggtacctt gtaaaagtcc tcatcaggga tcatggctct
     2221 ccctccctcc agcgctggta gaggtggtgg tcgatacaga gcacatccag gatcttaccc
     2281 accacgaagt ccaccaagtc ccccatatcc tcgggctggt ggtagaaggc tggcatggcc
     2341 ggcagtatga taacgccctc ccgggagagt ttcaacatat tttccaggtg aacctggcgc
     2401 aggggagttt cccgtggcac catcaccagc ttccggtgct ccttaagggc cacatctgca
     2461 gttcgggtca cagcgttact ggcatatccc tggctgatgg cggccacggt tttcatggtg
     2521 gcaggcacaa tcaccatgga ctcaaagcgg caggagccac tgttaatagc agcggtaagg
     2581 tccttagaat cgtagacacg gtacgctaaa ctctccaact ccgatgcact gatctccagt
     2641 tcgtagtcaa taataatccg ggccggctcc gtaaccagaa gagcagtttt cttaccggct
     2701 tctttaaggg cttccaggag ttgtcttgca atggacttaa attgatcccc atat
//
LOCUS       DAOQVW010000182         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43677_length_2751_cov_3.847552, whole genome shotgun sequence.
ACCESSION   DAOQVW010000182 DAOQVW010000000
VERSION     DAOQVW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43677_length_2751_cov_3.847552"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..848)
                     /gene="npdG"
                     /locus_tag="PKK85_04740"
     CDS             complement(177..848)
                     /gene="npdG"
                     /locus_tag="PKK85_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNS25359.1"
                     /translation="MKLGIIGGTGDQGLGIALRLAKAGEEIIIGSRDVKKAENAVSLV
                     KNMLADDETRNIRGATNQEAAREADLAVLTVPLQAQILTLKGIREELQGKIVVDATVP
                     LDSCLAGRPNRYVDVWEGSAAERSQAMLRDQEVKVVSAFNNMSAASLTNADKDVDCDC
                     LVSGDDPEAKKVVMELAEKIPGVRSIDCGALENARIVEKITPLLINLNIRNQIKMAGI
                     RITGL"
     gene            complement(944..1693)
                     /locus_tag="PKK85_04745"
     CDS             complement(944..1693)
                     /locus_tag="PKK85_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNS25360.1"
                     /translation="MKIGVSTLALYPEKLDNVLGYLEELKVDFCEIINEYPYHQVGSD
                     DLESFNIQATIHAPLSDINLASTNQAIQKASIMQVKRSMDLARSLDAEVVVVHPGQMP
                     ILSRGLEEAVLRYNQDSLAECAAYAFDCGVSMCVENMPRIDGLLFQDLAELHQLVQDL
                     DVHITLDVGHAHNNSIPPAEMLKSSRVKHVHLSDNDGSFDHHHALGSAGIDFKSIISH
                     LQELNYRGILVVEVKESADIKPSLSYLQGLL"
     gene            complement(1733..>2751)
                     /locus_tag="PKK85_04750"
     CDS             complement(1733..>2751)
                     /locus_tag="PKK85_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNS25361.1"
                     /translation="PGAGKTTFAQAVAEFYLNNGSIVKTMESPRDLQVEDEITQYAPL
                     EKDMGKTADILLLVRPDYTIYDELRKTKDFRIFADMRMAGVGMVGVVHATRPIDAIQR
                     IIGRVELGMIPSIVDTTIYIDEGQVKAIYQVTLTVKVPSGMVEADLARPVIEIRDFET
                     GSLAHEIYTYGEQTIVMDVGPERREKPPVHRIAEKEIQREIKKRVPQARIKVDMKSDR
                     RATVWIDEEYVAQLIGKRGKNIEEIEKRVGISIGVETLQSRDIYEQAPVEVDIAGNYV
                     VLQFGKEAVGTPFDILVNNDYLFTATVGKKGHIKIKKDIELAEIILKALQSNEPIGAR
                     VRRE"
BASE COUNT          606 a          772 c          600 g          773 t
ORIGIN      
        1 tcctatcaat tctgaccggc cacttccaac gatgaacact gactgggagt tcatgatggt
       61 atctatcatc aggttcacct tttgggggtc gattcgattg atgacctcca gggaatgttt
      121 tgcaatggat tctgcagtat tcttcagtat ctccatattt aacttccttt ataaatttaa
      181 aggccggtta tccttatacc tgccatctta atctggttac gtatgtttaa atttatgagg
      241 aggggtgtta ttttctccac gatgcgggca ttctccaggg ctccgcagtc gatggaccgg
      301 actccaggta tcttctcagc cagttccatt accaccttct tggcctcagg atcatctcct
      361 gataccagac agtcacaatc cacgtcttta tcagcgttgg taaggctggc cgcgctcatg
      421 ttattgaagg cggataccac cttcacctcc tggtccctta gcatggcctg tgatctctca
      481 gctgccgaac cctcccacac atccacgtag cgattgggcc ggcctgccaa gcaactgtcc
      541 aggggtacag tggcgtctac cacaatttta ccctgaagtt cttcccgaat ccctttcaga
      601 gtgaggatct gggcctgcag gggcacagtg agcacggcca gatctgcctc ccgggctgct
      661 tcctggttag tggcaccccg aatattccgg gtctcatcat cagccagcat attcttaact
      721 agactgacag cattttccgc cttcttaaca tcccgagatc caataataat ttcttcaccg
      781 gctttagcca gtctaagagc tattcccaag ccttgatcac cagttccgcc tataattccc
      841 aacttcatac tattatcccc actttaacta catactttcc acgaatctat taatagagaa
      901 tctttttctt cccactttat aaatcattat atctagccat gggttacagt aggccctgca
      961 ggtaactgag gctgggcttt atatcggcgg actctttaac ctccaccacc aggatccccc
     1021 ggtagttcaa ctcctggaga tggcttataa tggatttgaa gtcaatacca gctgatccca
     1081 gggcatggtg gtggtcgaat gatccatcgt tatcagaaag gtgcacatgt ttcacccggg
     1141 aggactttaa catctctgca ggggggatgg aattgttatg agcatgtccc acatccagag
     1201 ttatgtgaac atctaggtcc tgcaccaact ggtgcaactc cgccaagtcc tggaagagaa
     1261 ggccgtcaat gcggggcatg ttctccacgc acatactaac cccacagtca aatgcgtagg
     1321 cagcgcactc tgccagtgaa tcctggttgt atcttagcac tgcctcctcc aagccccggc
     1381 tcaggatggg catctgcccg ggatgcacca ccaccacctc agcgtccaaa ctcctggcca
     1441 ggtccatgga acgcttcacc tgcataatag aagctttctg aatagcctgg ttagtagagg
     1501 ccaagttaat atctgacagc ggggcgtgga tggtggcttg tatattaaat gactccaaat
     1561 catcactccc tacctgatgg taggggtatt cgtttattat ttcacagaaa tccactttca
     1621 gctcttccag gtagcccagt acattatcca gtttctctgg gtacaaagcc agggttgata
     1681 caccgatctt catttaaggt tctccagctg ttattttttc cttccctggc aactactccc
     1741 tcctcacccg ggctcctatg ggttcgttac tttgcagagc ctttaggatt atttctgcca
     1801 gttctatgtc tttttttatc ttaatatggc cttttttgcc cacggtggcg gtgaagaggt
     1861 aatcattgtt aaccaggatg tcaaatgggg ttccaacagc ttctttaccg aactggagca
     1921 ccacgtagtt cccagctata tccacttcca ctggggcctg ttcataaata tcacgggact
     1981 gcagtgtctc cacaccaatg ctgataccca cccgtttttc tatctcctcg atgttcttac
     2041 cccgtttacc aatcaactgg gccacgtatt cttcgtcaat ccacaccgtg gccctccggt
     2101 cagatttcat atcaacctta atccgggcct gggggactct cttcttgatc tcccgttgga
     2161 tctccttctc agctatgcgg tgcaccgggg gtttctccct acgctccgga cccacatcca
     2221 tgacaatggt ctgctccccg taggtgtaga tctcgtgggc caggctacca gtttcaaagt
     2281 ccctgatttc aattaccgga cgggccagat ccgcttccac cattccactg ggcaccttca
     2341 ccgttaatgt gacctgatag atggccttaa cttgtccctc atcgatgtag atggtggtgt
     2401 ccacaatgga ggggatcatg cccagctcca cccggcctat gatacgctgt atggcgtcga
     2461 tgggccgggt ggcgtggacc actcccacca tacccacccc agccatcctc atatcagcga
     2521 atattcggaa gtccttggtt ttacgtaact catcatatat ggtgtaatca ggtctaacca
     2581 acaacaatat atccgcagtt tttcccatgt ccttctcaag gggggcgtac tgggttattt
     2641 catcttccac ctgcaagtcc cggggtgatt ccatggtctt cactatggaa ccgttgttga
     2701 ggtagaattc tgccacggcc tgggcgaaag tggttttacc ggcaccaggg g
//
LOCUS       DAOQVW010000183         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43811_length_2746_cov_3.515422, whole genome shotgun sequence.
ACCESSION   DAOQVW010000183 DAOQVW010000000
VERSION     DAOQVW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43811_length_2746_cov_3.515422"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..712
                     /locus_tag="PKK85_04755"
     CDS             86..712
                     /locus_tag="PKK85_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25362.1"
                     /translation="MIEQEREQLERQVNELQRQKMDLEHQIQELDLEKLNKIETLKND
                     LERQVEWLDKDRIKLTKERDNLLRKIRISNEKKWKNALKMISLLIILDLIIIPLLLYV
                     MGLPIYWFFISLGLVTFFGMVVVVNYMSGTAPLNTGEIRKSLTVSFLAVYFAMISFMA
                     FGGASYLPDQPVSILVQSFTALMAILIGFYFGTRAIEKYVKAKKKDKS"
     gene            complement(764..2053)
                     /locus_tag="PKK85_04760"
     CDS             complement(764..2053)
                     /locus_tag="PKK85_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS25363.1"
                     /translation="MKDRYPIAVVGGGPAGCMAAIKASTAGEKVVLLEKNPDIGRKIL
                     LTGNGRCNLTNNSSLSVLLEKFGRRGSFFRTAFSTFTSNDLVSFFNKAGLKVKEEDGG
                     RIFPITDRSQSVNDVLRDQLKKGKVKIIYNYPVQELKKEDGYFQIKKAKNSGMKNKQK
                     KLIPPISDVILASRVILSTGGVSYPKTGSTGDGYQLARDLGHHITPLKPGLVPLKIKE
                     DWTRSLQGVVLKEVGLTFRHGKQGRIKGEGDVLFTHYGVSGPTILDLSQDVVKLLDRE
                     VEVKLFLDLIPTVTREKMEGILLADLKKHGKKDLQNYLRRYLPLGFIKPFLEEMAIDS
                     NKKLNQIHRRERITLLDNIKNLKLTVNGSLPLVKAMVTCGGISRREINPETMESRLVP
                     GLYLAGEIIAGCATSGGYNLQQAFSTGFLAGKGAGGL"
     gene            complement(2078..2746)
                     /gene="aspS"
                     /locus_tag="PKK85_04765"
                     /pseudo
     CDS             complement(2078..2746)
                     /gene="aspS"
                     /locus_tag="PKK85_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031709.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
BASE COUNT          712 a          675 c          608 g          751 t
ORIGIN      
        1 aattttcatc cgccacactt ataaacttaa ataatagtct ggacatagtt agatttagtt
       61 tttattattt ccagggggct tagccatgat tgaacaggaa agagaacaac tggaaagaca
      121 agtaaatgaa cttcaacgtc aaaagatgga tctggaacac cagatccagg agctggacct
      181 ggagaagcta aacaagatag aaaccctaaa aaacgacctg gaaagacagg ttgaatggct
      241 ggataaggat aggatcaaac tcaccaagga acgggataat cttctaagaa aaatacgcat
      301 ttccaatgaa aagaaatgga agaatgccct taagatgatc agcctgttga tcattctgga
      361 cctgattatc attcccctgc tgctttatgt aatgggcctc cctatttact ggttcttcat
      421 atccctggga ctggtgacct tctttggcat ggtggtggtg gtcaactaca tgtccggcac
      481 tgccccactc aacaccggcg aaatccgaaa atccctaaca gtgtcctttt tagcagtgta
      541 ctttgcaatg atatccttca tggcatttgg gggtgcatca tatcttccag accagccagt
      601 atccatcctg gtacaaagtt tcacagcatt aatggccatc ctaattggat tctactttgg
      661 aacacgggcc attgagaagt acgttaaagc aaaaaagaag gataaatcct aggggttaac
      721 cctggaaata ctattttttt acaaaaacca ctaattattt tttttaaagt ccaccagcac
      781 ctttacccgc tagaaaacct gtggaaaatg cttgttgtaa gttgtagcca ccactggtgg
      841 cacatccagc tatgatctcc ccggccaggt acaggccagg cactaggcgg gactccatgg
      901 tctcgggatt aatctccctt ctagaaatac caccacaggt aaccatggcc ttaaccaggg
      961 gtaaactacc attaacagtc aatttcagat ttttaatgtt atccagtagt gtaattcgtt
     1021 ctctacggtg gatctggtta agttttttat tagaatcaat agccatctcc tccaggaatg
     1081 gtttgataaa gcctaagggt agatacctcc gcagatagtt ttgcaagtct ttttttccat
     1141 gttttttaag atcagcaagt aggatcccct ccattttttc tctagttacg gttggtatta
     1201 ggtccaggaa caatttcact tcaacctctc tatccaggag cttaaccacg tcttggctca
     1261 aatccaggat ggtgggtccc gacactccat agtgggtgaa aaggacatct ccctcccctt
     1321 ttattctccc ctgtttgccg tgtcggaagg tcaaacccac ttctttgagc accacaccct
     1381 gcagggaccg ggtccaatcc tctttaatct tcaggggtac taggccaggt ttaagggggg
     1441 tgatgtggtg gcccagatcc cgggccaact ggtaaccatc cccggtggac ccagttttgg
     1501 ggtaagaaac accaccagtg gatagaataa cccgggaagc caggattaca tcgctaattg
     1561 gggggataag ttttttctgc ttatttttca ttccactgtt ctttgctttt ttaatctgga
     1621 agtacccatc ctcttttttt aattcctgaa ctgggtagtt gtagatgatc ttaaccttcc
     1681 cttttttcaa ctggtcccgg agcacgtcgt tcaccgattg ggaacggtca gttatgggaa
     1741 aaatccttcc tccatcttct tctttaacct tcaatcctgc cttgttgaag aaactaacca
     1801 gatcattact ggtgaaagtt gaaaaggcag tgcgaaaaaa tgatcctctc ctaccaaact
     1861 tttccaataa aacagagagg gaggaattgt tggtgagatt acaccgtcca ttacctgtta
     1921 aaaggatctt ccgccctata tccggatttt tctccagaag caccaccttc tcaccagctg
     1981 tgctggcttt aatagcagcc atacaccctg ctgggcctcc gcccaccact gcgatggggt
     2041 atctatcctt catttccatg atgtgataat aagggtttta tggtgttaat cgacgacggt
     2101 cccgggggaa taggactgtt tccctgatat ttcttgcacc ggtgaggcac atggtgaacc
     2161 gctccgcacc caacccccat ccggcatggg ggggcatacc atactggaaa gcagccaggt
     2221 aacggttgaa ggagtcaggg ttaagtccct tgctttttat gcgctccacc agaaggtcgt
     2281 gcagatgcac ccgcatggcc ccggaggaaa tctccaagtc ccggtacatg agatcaaagg
     2341 ccaggctgac ttgggggttt tcagggcggg gcatgacgta gaatggtttg atctcactgg
     2401 gccaatcact gatgaagtag tacccatcca taacttctcc tatagccttt tctgcagccc
     2461 gggaaaggtc ttcaccatag cggaggttaa ctcccttggc gttaaccagg ttcaccactt
     2521 ctgcgtactc caaacgtggg aagggagttt ctggtacctc cagttccacg cctatggttt
     2581 ccaactccta accacagttt tccttcacat cctttatggc ctgggccacc atttcctcca
     2641 ggaggatcat cacatcctcc tggtcagcga aggccacctc cacatcaatg gagatagcct
     2701 cgttaaggtg ccggagggtg tcatgctcct cggcccggaa gatggg
//
LOCUS       DAOQVW010000184         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43838_length_2745_cov_2.856506, whole genome shotgun sequence.
ACCESSION   DAOQVW010000184 DAOQVW010000000
VERSION     DAOQVW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43838_length_2745_cov_2.856506"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKK85_04770"
     CDS             <1..330
                     /locus_tag="PKK85_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNS25364.1"
                     /translation="RCPDPSCNPYLAFASMLEAGLDGIKNKVDPGDPVEIDVFELDEA
                     GLEEIGIKTLPSSLWEAYHALEKDEVIKKSLGEHIYPRFYELKRKEWDDYRIQVFRYE
                     QDKYLQI"
     gene            431..2122
                     /locus_tag="PKK85_04775"
     CDS             431..2122
                     /locus_tag="PKK85_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HNS25365.1"
                     /translation="MPQETDRKMMEILRILAEHKDILGAKTIAEYLKNRGYDLGERAV
                     RYHMGILDEKGFTERIGYAGRQITPDGLKELEKGLIYDQVDFIFSKLEDMMYHTTLDP
                     NTGQGNVVVNSSIFHYQEEIMDIISSAFEKGIAVSPLVKINQLETSSNLVKLETVCGT
                     TIDGMLLKAGIPVVPRYGGLVRVEDYSPLRFTELIAYKKTSMTPLEAFTNREMTSVLK
                     VISEGQGNIPANFRLIPGSAREKAMELFGKMEKIGITGLLKMGEKGENVLGIPVDNDM
                     VGIAVIGGITPLCAANEAGFDVKIKTAESSIELKYMKPLIRVKSILKPPSVNVPQKVK
                     FLLSKAWNFIEKVDFDLETHQGQVIANISLVDGELVDDVLEIFHRVMTSRPEYCTSKY
                     FQIVDGPNNKKGIATICSLTIDGILTKNGIASSPQYGGILETEGKIPRFIEFTAYSGS
                     SMDPHEIYLSRGMTSVLSSLDKGGKILASLKEIPYVARQNAVDVLERVEEAGFSILNI
                     GKPSELIYNAKVERYHVGIVAPGGLNPIAAVKESGIAVEAKAVETMMNVKDMEEL"
     gene            2192..>2745
                     /locus_tag="PKK85_04780"
     CDS             2192..>2745
                     /locus_tag="PKK85_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNS25366.1"
                     /translation="MDFFTYTLILLLVGLLVGFLAGLLGVGGGFIMGPVQFWLLSYLG
                     LDPNLAIRVAFATSLTATIPTAMSGAAGHYNKGAVDSKPALIIGIPSFLAAFLGAIIT
                     SKAPASTLSVVFGVVIIILALWMLRSENPDTTIQSRHMTPILIIIGLGTGLLSGALGI
                     GGGTILIPMLVMIMGCSMHRAIGTS"
BASE COUNT          853 a          566 c          616 g          710 t
ORIGIN      
        1 aggtgcccag acccatcatg taacccctat ttagcattcg catccatgct ggaagcaggg
       61 ctggatggta ttaaaaataa agtagatcct ggagatccag tggagatcga tgtgttcgaa
      121 ttggacgaag ctggtttgga ggagataggt ataaaaacct taccttccag cctctgggaa
      181 gcgtatcatg ctctggaaaa ggatgaagtt attaaaaaat ccctgggtga acatatatac
      241 ccccgttttt acgaattaaa aagaaaagaa tgggatgatt acaggataca agtattccga
      301 tacgaacagg acaagtactt gcaaatctaa ctccattttt ttcttttttt tagaatccta
      361 atttttttgc cattaaaaaa aggatttaat ctctcctccc actactttaa cagaaataga
      421 gggtgaagta atgccacaag aaacagaccg caagatgatg gagattttaa ggattctagc
      481 ggagcacaaa gatattctag gtgccaaaac catagccgaa tatcttaaaa atcgaggata
      541 tgaccttgga gaaagagcag tcagatacca catgggtatc ctagatgaaa aagggtttac
      601 agaacgaata ggctacgctg gacgtcaaat aactccagac ggtctcaaag aactcgaaaa
      661 aggcctaatc tacgatcagg tagattttat tttctctaaa ttagaggaca tgatgtacca
      721 taccaccttg gatcccaaca ctggccaagg taacgtggtg gtcaacagct ccatattcca
      781 ctaccaagag gaaattatgg acataataag ttctgcattc gaaaaaggaa tagcagtaag
      841 tccattagta aaaataaacc aactggaaac ctcatccaac ctagtaaaat tagaaacagt
      901 gtgcggaacc accatcgatg gcatgttact gaaggccgga atcccagtgg tgcctcgata
      961 cggtggtctg gttagagtgg aagattacag tcccctgaga ttcacagaat taattgccta
     1021 caaaaaaaca tccatgacgc ccctggaagc cttcaccaac cgcgaaatga cttcagtttt
     1081 aaaagtcatt tcagaaggac agggcaatat tcccgccaat ttcaggctaa tacccggttc
     1141 tgcccgggaa aaagccatgg aattgttcgg aaaaatggaa aaaatcggaa taaccggcct
     1201 tttaaaaatg ggggaaaagg gggaaaatgt tctgggaatc cctgtggaca atgatatggt
     1261 gggtattgca gtgatcggag gaattacccc tctttgtgcg gctaatgagg ccggtttcga
     1321 cgttaagata aaaaccgctg aaagtagcat tgaattaaaa tatatgaaac ccctgataag
     1381 agttaaatca atactaaagc caccttcagt caatgtcccc caaaaagtta agtttttact
     1441 ttccaaagcc tggaatttta ttgaaaaggt agatttcgac ttggaaaccc atcagggcca
     1501 ggtaattgcc aacatctcct tagtggatgg ggaattggtt gatgatgttc tggaaatatt
     1561 ccaccgggtg atgacatccc gacccgagta ctgcacaagc aagtacttcc agatagtgga
     1621 tggtccaaat aataaaaaag ggatagccac catctgcagt cttaccattg atggcatctt
     1681 gaccaagaat ggcatcgcat cttcccccca gtatggaggc atactggaaa ccgaaggaaa
     1741 aattccccga tttattgaat ttacagccta cagtggttca tccatggatc cccatgaaat
     1801 atatctttcc aggggcatga cttcagtcct tagttccctg gataaaggcg gtaaaatatt
     1861 ggccagcctc aaagagattc cctacgtggc caggcaaaat gcggtggatg tcttggaaag
     1921 ggtggaagag gcaggatttt ccatattgaa cattggtaaa cccagtgaat taatttataa
     1981 tgccaaagta gagcgctatc acgttggtat cgtggcacct ggaggtttaa acccgattgc
     2041 agccgttaaa gaatctggaa tagctgtgga agctaaagct gtggaaacca tgatgaatgt
     2101 aaaggatatg gaagaactct aaaacaattc ctcatacaat aaacatgtac cccccaaagt
     2161 tccttatcat ttactaaagt aggagaaaaa gatggacttc tttacatata cactaattct
     2221 gttacttgtc ggtttgctgg ttggattttt agccggcctt ttaggggtgg gtgggggatt
     2281 cattatggga ccagtccaat tttggctcct cagctacctt ggtttggatc cgaacctggc
     2341 cattagagtg gcctttgcca ccagcctgac cgccaccata cccaccgcca tgagcggagc
     2401 agcaggccat tataataaag gagcagtcga ttcaaaaccc gctttaatta tcggcatccc
     2461 cagttttctt gctgcttttc taggggcgat tattaccagt aaagctccag ccagtactct
     2521 tagtgttgtt tttggagttg tgatcattat actggccctt tggatgctgc gctcagagaa
     2581 tccggacact actatacaaa gtagacacat gacacctatt ttaataatta ttggacttgg
     2641 aactgggtta ttatccggag ccctaggtat tggtggtgga accatcctca tacccatgtt
     2701 ggtgatgatc atggggtgca gtatgcatcg agccatagga acctc
//
LOCUS       DAOQVW010000185         2740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43954_length_2740_cov_3.635382, whole genome shotgun sequence.
ACCESSION   DAOQVW010000185 DAOQVW010000000
VERSION     DAOQVW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43954_length_2740_cov_3.635382"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..611
                     /locus_tag="PKK85_04785"
     CDS             123..611
                     /locus_tag="PKK85_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25367.1"
                     /translation="MVSQIIEKLPARDDYFCETKYYSPRFAHSKDHSPPIGEGKYYSP
                     KYATSNTPPNSDVPPKNRCQNRVGCHVREAADLVSDSSPEIGKAHEEYYGSPPNSSLD
                     DLILFTSSYLEDNRKRYEGERYLVCNRCGGYYQLEDHEEADDFMGECECGGQLEYSYT
                     SD"
     gene            complement(608..931)
                     /locus_tag="PKK85_04790"
     CDS             complement(608..931)
                     /locus_tag="PKK85_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25368.1"
                     /translation="MEKLTPQDVLEVLDLFTRVPSLMLKALVIRQVNVVETFHEEVET
                     YKKNLRPEDLAKIETVISTPTPQLQEIIGQAYQEQPVKQLKILMDPGARPFLDRNLEA
                     LKKII"
     gene            1058..1447
                     /locus_tag="PKK85_04795"
     CDS             1058..1447
                     /locus_tag="PKK85_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25369.1"
                     /translation="MMVTDFLRNITKGGERHGGPKNCRDINHNPPDPSRDINKSPSPL
                     HNPDDDPVHQDQEDDDGYYSPAIYREDEEYYGSPPESIDDALILFTTSFIEEEVIRNC
                     IDEINHDYIRSKEWELLWLAAKSRFRV"
     gene            1774..2028
                     /locus_tag="PKK85_04800"
     CDS             1774..2028
                     /locus_tag="PKK85_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25370.1"
                     /translation="MTEQFYEKLREISKFLEREGDGISYISNKPDAYTIGTRKYSEKI
                     VEYLDKNVEAWEGSEPQLKEKKGKSKQEKKVGYLWKIPVG"
     gene            complement(2025..2225)
                     /locus_tag="PKK85_04805"
     CDS             complement(2025..2225)
                     /locus_tag="PKK85_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HNS25371.1"
                     /translation="MKSGRDRENVIAGALVLVVLKKDQHSGKLTRGVVEDVFTRSRHH
                     PHGIKVRLQDGRVGRVKEVIKS"
     gene            2422..>2740
                     /locus_tag="PKK85_04810"
     CDS             2422..>2740
                     /locus_tag="PKK85_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNS25372.1"
                     /translation="MWLKNILRILLVLILLGSILMVYVYIPGKDAETNLMLGEHHVLV
                     LAADPSEPRPGMGGIDMAFVVTVVDGDIKNMTAVYPGGMAHPTAAAPPELQAQGVQAL
                     LLHD"
BASE COUNT          825 a          643 c          560 g          712 t
ORIGIN      
        1 gaaccctgga tcattaaaag gtacttcact tcacaggttc aacgaattga tcagttgaat
       61 gcaaaagtat atattggtat aaaattataa tattgtttga catgaacaac ggagtggttg
      121 atgtggtatc ccaaattatt gaaaaactac ctgcaaggga tgattatttc tgcgaaacga
      181 agtactactc accacgtttt gcccactcaa aagatcattc cccccccatt ggtgaaggaa
      241 aatattattc accaaaatac gccacttcta atacaccccc aaattcagac gttcccccca
      301 agaaccgatg ccaaaacagg gtaggttgtc atgtcagaga agctgctgac ctggtcagtg
      361 actcctcacc tgaaatcggt aaagcccatg aagagtatta cggttccccc ccaaattcat
      421 ccctagatga cctgatactc ttcacctcct cttacttgga ggataaccgt aaacgctacg
      481 aaggtgaaag gtacctggtt tgtaatcgct gcggaggata ctaccaatta gaagaccatg
      541 aagaagcaga cgatttcatg ggtgaatgtg agtgcggggg gcagctggag tacagttaca
      601 ccagcgacta gataattttt ttaagggctt ccaaattccg gtccaagaat gggcgagcac
      661 cggggtccat gagaatcttc aactgtttta ctggttgttc ctggtaggcc tgccctatta
      721 tttcctgtaa ctggggagtt ggggtgctga tcacagtctc tattttagcc agatcctcag
      781 ggcgcaagtt ctttttgtag gtttcaacct cttcatggaa ggtttccacc acgtttacct
      841 ggcggatgac cagtgccttc aacataaggg atgggaccct ggtgaaaaga tccaagacct
      901 ccagcacatc ctgtggggtt aatttctcca tggttttcca ttgaccttga ttctacttaa
      961 caccttcctg gcattcatcc cccaggatca ttttgaatgc aaacctttat attgactata
     1021 tacaataata ttgtttaaag taaacaacgg agtttttgtg atggtaacag attttttaag
     1081 aaacatcacc aaaggtggag aacggcatgg tggccccaag aattgccgcg acattaacca
     1141 taatccacct gacccatcaa gggatataaa caagtccccc tccccccttc acaaccctga
     1201 tgatgatcct gtccaccagg accaggaaga tgatgacggg tactactcac ctgctatcta
     1261 cagagaagat gaagagtatt atggttcccc cccagaatca atagacgatg ccctaatact
     1321 cttcaccacc tccttcatcg aggaagaggt catcagaaat tgtatagatg aaatcaacca
     1381 tgactacatc aggtccaagg aatgggaact cctatggctg gctgctaagt cgagattccg
     1441 agtctaattc gatttatctg ctaaatagat acaaaaaagt aaccgccact ataaccaaga
     1501 taaatccggc taaaaaaagc caaccacgac tgttgctttc tctttttcta cgggcgaagt
     1561 gctgcaggta ttccatagcc tccttttgat ccttatccag catgtaccta atatttgtaa
     1621 tcaacaatat taataaagta ctactgagtc acagtaactt ttatccatag atggaacaca
     1681 tcaatatata tactaattat caggagaacg ccaaatctta cttaacccat tctagtaaga
     1741 ggtagaggca ccatatttct taggagaata actatgactg aacagtttta tgagaaatta
     1801 agagaaatat ccaagttcct agaacgagaa ggtgatggta ttagctacat cagtaacaag
     1861 cccgatgcct acaccatagg cacccgtaag tacagtgaaa agattgtgga gtacttggat
     1921 aaaaacgtgg aagcctggga aggcagcgaa ccccaactca aagaaaagaa gggtaaaagt
     1981 aaacaggaaa agaaagtagg ctacctgtgg aagatacccg ttggttaact ttttattact
     2041 tctttaactc ttcccacccg tccatcctgg aggcggacct tgattccgtg gggatgatga
     2101 cgggaacggg tgaacacatc ttcaaccaca ccccgggtaa gtttaccact atgctgatct
     2161 tttttgagga caaccaaaac tagagcacct gctataacat tttcacgatc cctaccactc
     2221 ttcatagtcc tgccccttaa cagattctgg gattatttcc cccttcaaca ccaattccaa
     2281 aatataaact cgttatcttc tagccagtag tgaattaatt tgggtaaaag ataatataca
     2341 ccttccgcaa tatgagaata taacttttta ttataacacc agaaaccatg ttaccaaatt
     2401 agacaatcag gtaggcaaat catgtggcta aaaaatattt taagaatcct cctagtactg
     2461 atcctgctgg gaagcatcct catggtatac gtatacatac ccggtaaaga cgctgaaaca
     2521 aatctgatgc tgggggaaca ccatgtcctg gttctggctg ctgatccatc cgagccacgg
     2581 cctggtatgg gaggcattga catggccttc gtggttacgg tggtggatgg tgacattaaa
     2641 aacatgaccg ccgtgtaccc gggtggaatg gcccacccca ccgccgcagc accccctgag
     2701 ttacaggccc agggtgtcca ggcccttctg ctccacgact
//
LOCUS       DAOQVW010000186         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_43968_length_2739_cov_8.764158, whole genome shotgun sequence.
ACCESSION   DAOQVW010000186 DAOQVW010000000
VERSION     DAOQVW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43968_length_2739_cov_8.764158"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKK85_04815"
     CDS             complement(<1..204)
                     /locus_tag="PKK85_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25373.1"
                     /translation="MVYKADNIEDTHLHYEKGIYLHQLNEDLNLFYHHQLHEFMIQYT
                     VKPITWTSKPWPHNQPLHQEPVKC"
     gene            complement(568..2370)
                     /locus_tag="PKK85_04820"
     CDS             complement(568..2370)
                     /locus_tag="PKK85_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25374.1"
                     /translation="MTHIDLQTTMKNQLILKITTKQPDEIKILIPNDGYHTCHDTLRL
                     EKPIHKVSSTSPWSTTLKAKINRAYQAMGETEAMAKMIIKEGYFDALMELQQQHHQKV
                     EAAQEMESLLIEQAIQKRETRIRKESQEFTEYLHRHNATVMDFLEFISTWLLSGESKN
                     IMTVFFAHFSTITGLRPIWTIFLGGPGEGKSAIEQASFSLIPARCKYGGRSSYAATLN
                     QARDYGNDFLDRKIISLGDLGGKNSYIKWEETLDVYKELSSEGAYDYKKMEDSVNPDT
                     KQKEVISIKVEGYPSVSFASTHSDGLTGQYLSRGITITPVGTDEDILNYRRYVRPATR
                     AKAFRDELTGPLMRLFHSYIENLLLDIETTEVINPYYLCLEDWFRGASNVKRASEMFT
                     LLVDAVTLFNYKSRHLLTTIDGDKYYLATKEDNEVIARLFDITPGLTAQVVAFYNKLV
                     ERVGKYDYDEFNDYKNGDKSINECKTIFTVSTLRHNRFRRASEDEKEQYSKFCQLLVD
                     AGKLDKLMKNHKGHNVYCLTDGELMGTVNMKCDISKVKEYIEEDISGNGWGMGLDRDE
                     IVACIGDEKSHRSSWTDFKQPPWDNGDKLMEGVE"
     gene            complement(2512..>2739)
                     /locus_tag="PKK85_04825"
     CDS             complement(2512..>2739)
                     /locus_tag="PKK85_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25375.1"
                     /translation="TVGNDTYKGVLLDNLKMPRSEEDRVQVYFITERGCGQPGYIRSR
                     AVEIIATGADPVIPSKVATPFSINELAGDPP"
BASE COUNT          661 a          625 c          580 g          873 t
ORIGIN      
        1 acacttcacg ggttcctggt ggagtggttg gttgtgtggc catggcttgc tggtccaggt
       61 gatgggtttg acggtgtatt ggatcatgaa ttcatgaagt tggtggtggt agaataggtt
      121 gagatcctcg ttgagttggt ggaggtagat gcctttttca tagtggagat gggtgtcttc
      181 aatgttgtcc gctttatata ccaaagttga tcatctccac ttttcggctg tcaattttgt
      241 acaactttga gggggaaagt tgtacacact tataaaggtt tataagtgaa agttgtacat
      301 ggtataacga agtagtaaca cttatctttt tattagtatg tcgagtatta caaccacctg
      361 gaataataat aaaatccatg ggaaattgta caacatccct tataaactgt acaacttcca
      421 acaccccaac tgtacaactt cccttataaa ctgtacaact tccaagcccg aaatcatgac
      481 ctttacttat aaagtgtaca actttcaaat cacaaccccc caaaaaactt ggaaaacaaa
      541 aaccatcaaa acacgttgat aactgaatca ctccacaccc tccattaatt tatctccatt
      601 atcccagggg ggttgtttaa aatcagtcca actactccga tggcttttct catcaccaat
      661 acaagccacg atctcatcac ggtccaaacc cataccccaa ccattaccac ttatatcctc
      721 ctcaatatac tccttaacct tggagatgtc acatttcatg ttaacagtgc ccattaattc
      781 cccatccgtc aaacaataga cgttatggcc tttatggttc ttcatcaatt tatcaagttt
      841 acctgcatct accaagagct ggcagaactt actatactgt tccttctcat cttctgatgc
      901 acggcggaac cggttatgtc tgagggtgga tactgtgaaa atcgttttac actcattaat
      961 actcttatca ccattcttgt agtcattaaa ctcatcataa tcgtatttac ccacacgctc
     1021 cactaattta ttatagaagg ccacgacctg ggcagttaaa ccaggagtaa tatcaaaaag
     1081 cctggctatg acttcattat cctccttagt cgccaggtaa tacttatccc catcaatggt
     1141 agttaataga tgcctgcttt tataattaaa taatgtaacc gcatccacca ggagggtgaa
     1201 catttcactg gcccttttca cattacttgc accacggaac caatcctcca aacacaggta
     1261 gtagggattg ataacctcag tggtttcaat atccaataat aagttctcaa tgtagctgtg
     1321 gaataacctc ataagtgggc cggtgagttc atcccggaaa gccttagccc gtgttgctgg
     1381 ccgtacatat cggcggtagt taagtatgtc ttcatcagta cctactgggg tgatggtgat
     1441 accccggctt aggtactggc cggttaatcc atcactatgt gtactagcaa aactgacact
     1501 ggggtatcct tctactttga tgctgataac ttccttctgt ttggtgtcag ggtttacgct
     1561 gtcttccatc ttcttatagt cataggctcc ttcgctgctg agttctttgt acacatccag
     1621 ggtttcctcc catttaatgt aactgttctt acctcccaga tcccccaggc ttatgatctt
     1681 ccgatccagg aagtcattac cataatccct ggcctggtta agtgtcgccg cataactact
     1741 cctgcccccg tatttacacc ttgcaggtat taggctaaaa gatgcttgtt cgatggcact
     1801 ttttccttca cctggtcctc ctaggaagat ggtccagatg ggtcggagtc cggtgatggt
     1861 gctgaagtgt gcgaagaaaa cagtcataat atttttactt tctccactga gtagccaggt
     1921 actgatgaac tccagaaaat ccatcacggt ggcattatgt ctatgcaagt attcagtgaa
     1981 ttcttggctt tctttgcgta ttcgtgtttc tcgtttctgt attgcttgtt ctattagaag
     2041 ggattccatt tcctgggcgg cttctacttt ttggtggtgt tgttgttgta gttccatgag
     2101 ggcatcgaag tagccttcct tgattatcat tttggccatg gcttcggttt ctcccatggc
     2161 ttggtaggcc cggttgattt tggcttttag tgtggtgctc caggggctgg tgctgctgac
     2221 tttgtgtatg ggtttttcta gtcgtagggt gtcgtggcag gtgtggtagc cgtcgttggg
     2281 tatgaggatt tttatttcgt ctggttgttt agtggtgatt tttaggatta gttggttttt
     2341 cattgtggtt tgtaggtcta tgtgtgtcac ctcctttgca gctggttaat aggtgtcatg
     2401 gtttctatcc gtatttttgt tggagtttca tcccgattag gactccgaac tggcagacaa
     2461 ctaccaatac caggatgact agtagatagt ctagccagga taggaatatc atcatggcgg
     2521 gtcacctgct agttcgttga tgctaaaagg agttgccacc ttagagggga tcactggatc
     2581 cgctccggtg gcgattatct ccacagcacg actccttata taacccggct gaccacaccc
     2641 cctttcggtg atgaagtata cctgcacccg gtcctcctct gaccggggca tcttcaaatt
     2701 atccagtaag acacccttgt aggtgtcatt ccccacggt
//
LOCUS       DAOQVW010000187         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_44023_length_2737_cov_3.049963, whole genome shotgun sequence.
ACCESSION   DAOQVW010000187 DAOQVW010000000
VERSION     DAOQVW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44023_length_2737_cov_3.049963"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKK85_04830"
     CDS             complement(<1..333)
                     /locus_tag="PKK85_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HNS25376.1"
                     /translation="MNDENRLKGTTTVGLTCKDGVVFATERRASMGNLIAHKVADKIF
                     KLDHHIGATIAGSVSDAQSLMKYIGAEIALYRLRNGKKMSVEAAATLTSNILHSSRFY
                     PFFVQTLLG"
     gene            complement(548..1639)
                     /locus_tag="PKK85_04835"
     CDS             complement(548..1639)
                     /locus_tag="PKK85_04835"
                     /EC_number="6.3.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HNS25377.1"
                     /translation="MSKIDRQKIMDVLDGYDKEDITIATLGSHSSLHMFQGAKTEGFR
                     TAVVCEKGREVPYKRFNVADEYIMVDHFSDIVNEEVQEELRDLNSIVFPHGSFVAYAG
                     LDNIETIFNVPMFGNRDILRWEAERDLERKLMIESGIRIPQKISNPEKIGGTVMVKFP
                     GARGGRGYFVATSTEEYEEKIEAMLTRKWIEEEDIKEAHIEEYVLGCNYCIHYFFSGL
                     KDEVELMGIDSRYESTIDGLVRVPAKDQIDIGADPSYVITGNHPVVMRESLLPQVFDI
                     GDKITETAKGLVPPGFNGPFCMQTLVNDDLEVVVFEMSARSDGGTNTFMNGSAYSYLY
                     YGEPLSMGRRMAIEIKNGLAEDRLEEIIT"
     gene            complement(1861..2388)
                     /locus_tag="PKK85_04840"
     CDS             complement(1861..2388)
                     /locus_tag="PKK85_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016358078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNS25378.1"
                     /translation="MMIDKVLKANEEFTQEFEPKKMSHMPQKKLSIVTCMDTRLTGFL
                     EPALGIERGDAKIIKNAGNTVAGRDVIRSVAASIFALGVEEVMIIGHTNCGMANVDPK
                     KLESAMEERGIDPEEIAKVDIKEWIGAIDSEESNVIQVVEQIKSSPLIPDDVPIHGLI
                     IDIESGELKVLVDDR"
     gene            complement(2435..>2737)
                     /locus_tag="PKK85_04845"
     CDS             complement(2435..>2737)
                     /locus_tag="PKK85_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25379.1"
                     /translation="REACPLCWCKECAIESESDHWVGKGVIPPSPMYHFVRMVHMVDS
                     CTNCGQCEEVCPVDIPLAKMFHKINRNIQDVFDYRPGYDMEQKPPLSVIDKEPSEE"
BASE COUNT          775 a          600 c          473 g          889 t
ORIGIN      
        1 tcccaatagg gtctggacga agaagggata aaatctggat gaatgtaata tgttggaagt
       61 taacgtggca gcagcctcta cactcatttt tttcccattt ctcagccggt agagtgctat
      121 ttcagcccca atgtatttca tgaggctttg tgcatcagaa actgagccag caatggtggc
      181 tccaatatga tggtcaagtt tgaatatttt atcagccact ttatgggcga tcaagttacc
      241 catactggct cgtctttcag tggcaaaaac aactccatct ttacaagtta aaccaacagt
      301 tgtagtgcct ttaagtcgat tttcatcatt catagataca cctctattaa aataaatgtg
      361 aagtttaaaa aataagtttt ctaatggcta ctttaatatt catggtatat aaatattact
      421 agttaaggct tttacccttc aagggccttt taaagacctt ttttttaaag tttataccat
      481 tataaaattg ttttaagaca tggtagagcc attataaaaa tcaaaaaaaa tataaaaaaa
      541 ggattagtta ggtgatgatt tcttctaatc tatcttcagc caacccattt ttaatttcaa
      601 tggccattct acgtcccatg ctcaatggtt ctccatagta gaggtagctg taggctgatc
      661 cattcatgaa ggtgttggtt ccaccatcag acctggcact catctcaaac accactactt
      721 ctaagtcgtc gttaactagg gtctgcatac agaagggacc gttaaatccg ggtggtacca
      781 atcctttagc agtttctgtg atcttgtctc cgatatcaaa tacttggggt aacaatgatt
      841 ctctcatcac tacgggatgg ttacctgtta taacgtagga tggatccgca ccgatatcga
      901 tctggtcctt ggcaggaact ctgactagac cgtcaatggt ggattcgtat ctgctatcga
      961 tccccataag ttctacttca tcttttaatc cggagaaaaa gtagtgaata cagtagttac
     1021 accctaatac gtattcttca atgtgggcct cttttatgtc ctcttcttcg atccattttc
     1081 tagtcagcat ggcctcaatt ttttcttcat actcttcagt tgaggtcgct acgaagtaac
     1141 ctctgcctcc ccgggctcct ggaaacttca ccattactgt accgccaatt ttttcagggt
     1201 ttgatatctt ttgaggaatt ctaattcctg attcaatcat taactttctt tccaaatctc
     1261 tttcagcttc ccatcttaaa atatccctat ttccaaacat ggggacgtta aaaatggttt
     1321 ctatattatc caatccagca taggccacaa aggatccatg tgggaataca atactgttca
     1381 aatcccttaa ctcttcctga acctcttcat taactatgtc actaaagtgg tcaaccatta
     1441 tgtattcatc agcaacatta aaccttttgt aaggaacttc ccgccccttt tcacagacca
     1501 ctgcagttct gaagccctcg gtctttgctc cctgaaacat atgtaaggat gaatggctcc
     1561 ctaaagttgc aatggtaatg tcttctttat catacccatc cagaacgtcc ataatttttt
     1621 gccggtctat tttgctcatg gtttatttcc tccacaaatt cacaaattcc ccactataca
     1681 atgagttaat agaatatgga ttgtatgggg tagttatttt tttcgtcttg atctttcgta
     1741 tcactattca taaaaatagt gatatttata ttaaaaataa attccatgga aaaataccaa
     1801 ataactccat ttaaatagat agatcaagat ttttataaga aaaaaataga aaaaagggca
     1861 ctacctgtca tcgactaaga cttttaattc tccactttca atgtctatta tgagtccatg
     1921 tatgggtaca tcatccggaa ttagtggaga acttttaatc tgttcaacta cttggataac
     1981 gttggattct tcactgtcaa tggcacctat ccattctttg atatcaactt tggctatttc
     2041 ttccgggtca atcccccgtt cttccatggc gctttcaagt tttttcgggt caacgttggc
     2101 catgccacag ttggtatgac cgatgatcat cacctcttcc acccccaatg cgaatatgga
     2161 ggccgccaca gatcggatca cgtctctgcc tgccacggtg ttaccagcat tttttatgat
     2221 cttggcatcg cctcgttcga taccaagagc tggctctaaa aatccagtga gccgggtgtc
     2281 catacaagta acaatggaaa gcttcttttg aggcatatga ctcatttttt taggctcaaa
     2341 ttcctgggta aattcctcgt tagcttttag taccttgtca atcatcatgg tatcaccaaa
     2401 aaattattca aaaaaataaa tagatgtgag gaaatcattc ctcacttggt tctttgtcaa
     2461 tcactgagag aggtggtttt tgttccatat cgtatcccgg gcgataatca aagacatctt
     2521 ggatattgcg gttgatttta tggaacattt tggctagtgg aatatctaca ggacaaactt
     2581 cctcgcattg tccacagttg gtgcaggaat ccaccatatg aaccattcga acaaaatgat
     2641 acattggtga cggaggaatc acaccctttc ccacccaatg atcagactcg gactcaattg
     2701 cacattcttt acaccagcat aatggacaag cttctct
//
LOCUS       DAOQVW010000188         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_44352_length_2725_cov_3.034457, whole genome shotgun sequence.
ACCESSION   DAOQVW010000188 DAOQVW010000000
VERSION     DAOQVW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44352_length_2725_cov_3.034457"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..740
                     /locus_tag="PKK85_04850"
     CDS             63..740
                     /locus_tag="PKK85_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNS25380.1"
                     /translation="MCDANGYDTISQKLKDNLGLKRSPVAIKFVLRENDLPEGIPKIS
                     DKIRHCEMVLKASEGESFYATAEEQMCKGGAAALGLMEPLEKIKTGEFYQELGRFSSI
                     GSARKTMDAIPKIENIMYALVYAPLELANFDPDVIVIFSNPAQAMKLSQALIYTMGGR
                     VEADFAGIQSICADAVAGPFIRRKSNITLGCSGSRQYADVKDDEVIVGLTGENIGCVV
                     NALESMA"
     gene            complement(823..1494)
                     /locus_tag="PKK85_04855"
     CDS             complement(823..1494)
                     /locus_tag="PKK85_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNS25381.1"
                     /translation="MKPISAIVTAAGKNRRMRQDLKARGIPYRHKLLLEIDGEPVLTK
                     TIRNVLDTDISECLVVLGHYQEELLPVLDDVVDRRLRIIFNTDRNVELSQTILNAVLN
                     VVTDYCLCVAADQPSVTTSTLETLVNTLLNSEEPENTISILARRAVGQLDSAKGLGMP
                     FACHKNILMRFLPLDFDNLNPILVKMIQKDVKFYGVPHQNEVELLNINRYDDYLKVLE
                     LLKKE"
     gene            complement(1508..2695)
                     /locus_tag="PKK85_04860"
     CDS             complement(1508..2695)
                     /locus_tag="PKK85_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25382.1"
                     /translation="MTNLKNSYLGILVMIILAFVMFPLGNMIENKFPAQKPDEITSMN
                     LGETVLAGTKVVDDFKVRKTSSIYHPNYIIDLIYEGKYFKSFTALSTGTVETPFQEIT
                     GETISSQGIAQGFKGPGHLTVRDDKLVVNPPDTFVWGFITPYTIGVKTKNGLEIQENG
                     EIVKTVSLSDMSNSTIPHDYVSYNDFEKWYNKSDVGDKITLDYQISRFSDGRNPVAPS
                     QIVTYFGAGVKEYMQQYPSGSPVMVYNGATSQQVISSAVDIMGSYPEYNDNTRALNGK
                     QFARGWNGTIIPPHTASSGKDDIIFYGVYDKNASGDGRGGYVSHGACPPGRSLRSAVM
                     KVGFPLPRGMTGGHYSVAMESHPTTGIFVNNTSNFPVQIIMWTDGSGPSMRIYTQVIQ
                     FIP"
BASE COUNT          747 a          577 c          575 g          826 t
ORIGIN      
        1 agtacaggaa tgatagaaaa ttttatgctc atccctatcc aacttcaaat aggtgaatta
       61 atatgtgtga tgccaatgga tacgatacca tatcccagaa attaaaggac aatctaggac
      121 taaaaagatc tccagtagct ataaaatttg ttttaaggga gaatgatcta ccagaaggga
      181 ttcccaaaat atctgataag atcaggcact gtgaaatggt tttgaaggcc agtgaagggg
      241 aaagctttta tgctactgcc gaagaacaga tgtgtaaggg tggagccgcg gccttgggtc
      301 tgatggaacc tcttgaaaag attaagactg gagaattcta tcaggaactt ggtagatttt
      361 ctagcatagg atccgcacga aaaaccatgg atgcaatccc aaaaattgaa aacatcatgt
      421 atgctttggt ttacgctcct ttagaattag ccaattttga tccagacgta attgttatat
      481 tctccaatcc ggcccaagcc atgaaactat ctcaagccct catatatacc atgggtgggc
      541 gagtagaggc tgattttgca ggtatacagt ccatatgtgc tgatgcagtg gcaggtccct
      601 ttatccgacg aaaatccaat atcacccttg gttgtagtgg atcccgccag tacgcagatg
      661 ttaaagatga tgaggtaatc gtaggtttga ctggagagaa tattggctgt gtggttaacg
      721 ccctggaaag tatggcttaa ataattaatt atattgtctt agtttagtgt gagatcttag
      781 gatctcatcc tcttttacca tgcctctgaa aattttttca acttattctt tcttaagtaa
      841 ttctaaaacc ttcaaatagt catcatatct gttgatgttt aaaagctcca cttcattctg
      901 gtgtgggacg ccatagaact taacatcttt ctggatcatc tttacgagga tggggttgag
      961 gttatcgaaa tccagtggta ggaagcgcat caggatgttc ttgtgacagg caaatggcat
     1021 gcccagccct ttggcagaat ccagttgtcc cactgccctc cgagccagga tggatatagt
     1081 attttccggt tcttcagaat ttaaaagggt attaaccaaa gtttctaagg tggaagtagt
     1141 tactgagggc tgatcagcag ccacgcataa acagtaatca gtcacaacat ttaaaacggc
     1201 attgagaata gtttgtgata actccacatt cctgtccgtg ttgaatatta tcctgaggcg
     1261 cctatcaacc acatcatcca gaactggcag taactcctcc tgataatggc ccaaaaccac
     1321 taaacactca cttatatctg tatctaggac gtttctgata gttttagtta aaactggttc
     1381 gccatcaatc tccaggagaa gtttatgcct gtaaggtatt cctcttgcct taagatcttg
     1441 tctcattctg cggtttttcc cagcagcagt tactatggca gagatgggtt tcaagatctt
     1501 cactccgtca tggtatgaac tgtatcacct gggtgtatat gcgcatgctg ggtccacttc
     1561 catcggtcca catgatgatc tgtaccggga agttactggt gttgtttaca aatataccgg
     1621 tggtagggtg gctttccata gccactgagt aatgtcctcc agtcatacct cggggtaatg
     1681 gaaatcctac cttcattacc gctgatctta aggaacgtcc cggggggcat gcgccatgac
     1741 ttacataccc tccccttcca tctcctgagg catttttatc atacacaccg tagaagatga
     1801 tatcatcttt tccactagag gctgtgtggg gggggattat ggtaccattc catcccctgg
     1861 caaactgttt accattcaaa gcccgggtgt tatcgttgta ttctggatac gatcccatga
     1921 tatcaactgc actgcttatc acctgctggc tggtggcgcc gttgtagacc atcaccgggg
     1981 aaccactggg gtactgttgc atgtactcct ttactcctgc tccaaagtat gtcacaatct
     2041 gggaaggcgc aactgggttc cgcccatcac tgaaacggga tatctgatag tccagggtga
     2101 tcttgtcacc tacatcagat ttattgtacc atttctcgaa atcgttatag ctcacatagt
     2161 catgggggat ggtgctgtta ctcatatctg atagagacac agttttcaca atttcaccat
     2221 tttcctggat ttccaatcca ttcttagttt tcacgccaat ggtgtagggc gttataaatc
     2281 cccatacaaa agtgtctggt ggattgacca ccaatttatc atccctaacg gtaagatgac
     2341 ctgggccttt aaatccctgg gcaattccct gggaagagat agtttcccct gtaatctcct
     2401 ggaagggagt ttctacagtt ccagtggata gggcagtgaa agatttgaaa tactttcctt
     2461 cataaattaa atcaattata taatttggat ggtaaatgct ggaagtttta cgtaccttaa
     2521 aatcatccac taccttggtt cctgccagca cagtttctcc taaattcatg gaagttattt
     2581 catcaggttt ttgtgctgga aatttatttt caatcatatt tcctagggga aacatgacaa
     2641 atgccaatat tatcattact aaaatgccta aatacgagtt tttaaggttt gtcatccttc
     2701 acaccttttt ttataagttc aaaga
//
LOCUS       DAOQVW010000189         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_44803_length_2706_cov_3.002263, whole genome shotgun sequence.
ACCESSION   DAOQVW010000189 DAOQVW010000000
VERSION     DAOQVW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44803_length_2706_cov_3.002263"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKK85_04865"
     CDS             complement(<1..419)
                     /locus_tag="PKK85_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS25383.1"
                     /translation="MTLTTNKDYDLCVAYRIYPRVSKVPPVFKDDKYKLSEFCLKSFK
                     DSLGDLKAKIFVLLDGCPPEYDELFRKYFMDEDLELIRLEGVGNLATFSLQIKVLLEQ
                     EFSDLVYFAEDDYFYMPNQFNEMIELLKENDDVDFVSP"
     gene            complement(457..1407)
                     /locus_tag="PKK85_04870"
     CDS             complement(457..1407)
                     /locus_tag="PKK85_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS25384.1"
                     /translation="MKQVLITGAAGFIGSHLCDRFIKEGWYVVGVDNFLTGSLENLEH
                     LLLNQNFELVEEDVIKGLEWEGHLDLILHFACPASPVDYLNFPIETLMVDSVGTSHTL
                     KLAKDKRARYVFASTSEVYGDPQVHPQPESYWGNVNPVGPRSVYDEAKRFSEALSMAY
                     HRECGVDVRIIRIFNTYGPRMKLKDGRVVPNFIFQALNGQDLTVYGDGSQSRSFCYVD
                     DLVEGIFLVSDVDAIKYRVMNLGNPDEYKIIDFAQIILEKTGSNSKIIFKPLPRDDPK
                     MRKPDISLARESLHWKPVISLDEGLAKTIQYFQSQLEDVE"
     gene            complement(1420..>2706)
                     /locus_tag="PKK85_04875"
     CDS             complement(1420..>2706)
                     /locus_tag="PKK85_04875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNS25385.1"
                     /translation="VGLVTAACFADLGNQVLCAKKTSRNLDKLNQGISHIYEPGLDEI
                     LKRCLDKGTIEFTTDMKRAIDFSDLIFICVGTPQSETGKADLSQVEDVARQIAENMTG
                     YKLIVEKSTVPVNTHQWVKKTIKRYSKEDIQFDVASNPEFLREGNGVHDFNNPDRIVV
                     GVETDNARGIFQELYKPFTEKGHMLLITSPAAAELIKHASNSFLAMKISYINMVAELC
                     EKVGIDVNMIADGIGYDQRIGRSFLNAGIGYGGSCFPKDLRAFIKIAEDHGLDFGLLR
                     ETEKINTQMRERFLEKIENVLWINKDKNITIWGLAFKPDTDDIREAPSIEIVRKLYET
                     DANLRLYDPQALKNFQNVFPENENIRYYSDKYEALKDSDALLVITEWDEFRSADLNKV
                     KDLMRLPIIIDGRNLFESEAMDGFEYYSVGREKNVP"
BASE COUNT          707 a          644 c          549 g          806 t
ORIGIN      
        1 ggagatacaa aatccacatc atcattctct tttaagagtt ctatcatctc attaaactgg
       61 ttgggcatgt agaagtaatc atcctcagca aaataaacta gatcagaaaa ttcctgctcc
      121 agcaacactt taatttggag agaaaaggtg gccaagttac ccaccccctc taatcttatc
      181 agctctagat cttcatccat aaaatatttc ctgaagagct catcatactc aggcggacac
      241 ccatccagca aaacaaagat cttagccttt aaatctccca gactatcttt aaaggatttt
      301 agacaaaact cggatagttt atacttatca tccttaaaaa caggggggac tttagaaacc
      361 cgggggtata tccggtaggc tacgcataga tcatagtctt tgttggtagt tagggtcata
      421 aactcaccat aaatcccata ataatgagtg cccttgtcac tctacatcct ccagttggga
      481 ctggaaatat tggatggttt ttgctagacc ttcatccagg gagatcactg gtttccagtg
      541 gagtgattca cgggccaggc taatgtctgg ttttctcatc ttaggatcat ctcgaggtag
      601 gggtttgaag attatcttag aattagaacc agttttttct aggattatct gggcaaagtc
      661 aattattttg tactcatcag ggttgcccag attcatgacc cgatatttaa ttgcatccac
      721 atctgatacc agaaaaatcc cttcaactag atcatccacg taacaaaagc tacggctttg
      781 gcttccatca ccataaactg tgaggtcctg accatttaag gcctggaata tgaagttagg
      841 cactaccctg ccgtctttta acttcatacg agggccatag gtgttgaaga tcctgatgat
      901 cctgacatcc acgccacact cccgatggta agccatggat aaagcctcag aaaacctctt
      961 agcttcgtcg taaacggaac gaggccccac tggattgaca tttccccagt aactttctgg
     1021 ctggggatga acctggggat ccccataaac ctctgatgtg gatgcaaaca cgtacctggc
     1081 cctcttatcc ttggccaatt tcagggtatg ggaggttcca actgaatcaa ccatgagtgt
     1141 ttcaatggga aaatttagat agtcaactgg actggctggg caggcaaagt gtaatatcag
     1201 gtccaagtgg ccttcccatt ccaatccctt tatgacatcc tcttcaacga gttcaaaatt
     1261 ctggttcagt aggaggtgtt ccaggttctc caagctccct gtcaggaaat tatcaacccc
     1321 aaccacgtac catccctcct taataaaccg gtcacagagg tggctcccaa taaaaccagc
     1381 ggcaccggtt atgagaactt gtttcattta agttttctcc tagggtacat ttttctccct
     1441 accaacactg taatactcaa atccatccat agcctcagat tcaaacaagt tccggccatc
     1501 aataattatg ggcagcctca tcagatcttt tactttattt aaatctgcac ttcggaattc
     1561 atcccattct gtgattacaa gaagggcgtc ggaatctttt aaagcctcat atttatctga
     1621 atagtacctt atgttctcgt tttccgggaa cacattctga aagtttttca gggcctgggg
     1681 gtcgtacaat cgtaaattag catcggtttc ataaagtttt ctcactattt caatggacgg
     1741 cgcctctctg atatcatcgg tgtctggctt gaaggccaat ccccagatgg ttatattttt
     1801 gtctttattg atccagagca cattttctat tttttccaaa aatcgttccc gcatctgggt
     1861 gttgatcttt tctgtttccc ttaaaagacc aaagtccaat ccatgatcct ctgctatctt
     1921 gatgaaggcc cgaagatcct tggggaaaca ggagccacca taaccgatac ccgcgttgag
     1981 gaatgatctt ccgatgcgct ggtcgtatcc aatgccatcg gcgatcatgt taacgtcaat
     2041 acccaccttt tcacacagct ccgccaccat gttgatgtag gaaatcttca tggccagaaa
     2101 ggagttcgat gcgtgtttta taagttcagc ggcggcgggg ctggttatga ggagcatgtg
     2161 tcctttttca gtgaatggct tgtagagttc ttggaatatt ccacgggcat tgtcagtttc
     2221 cacccccacc acaattctgt ccgggttgtt gaagtcatgg acaccgttac cttcccttag
     2281 aaattcgggg tttgaggcca catcgaactg gatatcctcc ttagaatatc tttttatggt
     2341 ctttttaacc cattgatggg tgttcacagg tacggtggac ttttcaacga tcagcttata
     2401 tccagtcatg ttctcggcta tttgccgggc cacatcctcc acttgggaca aatcagcctt
     2461 cccggtttca ctctgggggg tgcctacaca tatgaagata aggtcactga aatctatggc
     2521 ccttttcatg tcagtggtga attcgatggt gcccttgtcc aggcatcttt ttagaatttc
     2581 atctaaaccg ggttcataga tgtgtgatat tccctggttg agtttgtcta aatttcgtga
     2641 agtttttttg gcgcataaaa cctggtttcc cagatcagca aaacatgccg cggttactaa
     2701 tcccac
//
LOCUS       DAOQVW010000190         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_44846_length_2704_cov_2.954700, whole genome shotgun sequence.
ACCESSION   DAOQVW010000190 DAOQVW010000000
VERSION     DAOQVW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44846_length_2704_cov_2.954700"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKK85_04880"
     CDS             <1..230
                     /locus_tag="PKK85_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25386.1"
                     /translation="GFLSIIFPGMEWKIDGMDENIFFWGSESAMVLGFCLTRNTIPRP
                     TLDEHLHLYNTFYILRIKTILFLVKEMGGFL"
     gene            complement(394..885)
                     /locus_tag="PKK85_04885"
     CDS             complement(394..885)
                     /locus_tag="PKK85_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25387.1"
                     /translation="MPDWMVHVAVAWTLCRVLRFRHTQFDTPITILVMVGSLMPDVVK
                     IVMLFNTMGHDWWSYIYALHQPLGSFLVAAFACLFFEKKRDVFLFFSLGVLSHYALDL
                     FLLQISGGLYLFFPFNWICFSLDLVPNDDYTVTVVALGVALLVYVLGRWVEKKQDFLG
                     VTN"
     gene            complement(887..1348)
                     /locus_tag="PKK85_04890"
     CDS             complement(887..1348)
                     /locus_tag="PKK85_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25388.1"
                     /translation="MNLESPRSRILVGFLVLGVIVALCAYYAAEYENHLEHPSYRAIL
                     AEYPEGEVVRVYGTVVENYPGGYQIQQIYHGQVVSVEINGTPVAIGDRVDLLGVLGPE
                     YSIISVEKIYVNQMWKYHFLIIRSFLVLLFMIFIFHRYWYLDSRRFLFRRR"
     gene            complement(1625..>2704)
                     /locus_tag="PKK85_04895"
     CDS             complement(1625..>2704)
                     /locus_tag="PKK85_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS25389.1"
                     /translation="SLIETALDPLVTIGADGKITDVNRATEKTTGRSREELIGTDFAD
                     YFTQPEDAERGYQRVFQEGEVKDYPLEIKHQDGSITPVLYNASVYHDEDGEVVGIFAA
                     ARDITERKQAEEELRIYWESLEKEVRLRTEELARSNSDLQQFAYIASHDLREPLRMIS
                     NFLQLLERRYQDQLDQDAQDFIYYAVDGAKRLDEMINSLLDYSRIANQEIQRSPVDFQ
                     EVLEEVSFNLNVLIRENQARIDCQDLPILEADKTQMVRIFQNLISNSIKYRGEEAPWI
                     RVKAREEGDQVVFSVQDNGIGIDPQHLESIFTIFSRLHRQDQYPGTGMGLAIAQRIVH
                     QHGGEIWAESEGDGSTFYFSLPMWP"
BASE COUNT          659 a          715 c          677 g          653 t
ORIGIN      
        1 taggcttttt atccatcatt tttccgggga tggaatggaa aatagatgga atggacgaga
       61 atattttttt ttgggggtca gaaagtgcaa tggttttggg tttttgtctg acaagaaaca
      121 caatccctcg tccaacctta gatgagcacc tccacttata taatactttc tatatacttc
      181 gtattaaaac aatacttttt ttggtcaagg agatgggggg cttcctgtaa caccattcat
      241 actattaaaa aaaaaggatt ttcctggaac ccaacccagg gggcgaactg gtaaatctcg
      301 atttattagg aaatagccgc gaagatgctg tatataaaaa aaatctgcga ggcttaagaa
      361 atggtgttct tgatccataa gggtcctgga tccctaatta gtcacaccta aaaaatcctg
      421 cttcttctcc acccacctac ccaacacgta gaccagcagg gccaccccca aggccaccac
      481 tgtaacagtg taatcatcgt tgggcaccag atccaggctg aagcagatcc aattgaaggg
      541 aaaaaacagg tacagacccc cacttatctg cagcaggaac aggtccaacg cgtaatggct
      601 tagtactccc aggctgaaaa acaggaaaac atccctcttc ttctcaaaaa acagacaggc
      661 aaaggcagca accaggaagg atcccagggg ctggtgaagg gcgtagatgt aactccacca
      721 gtcatggccc atggtattaa agagcatcac tattttaacc acgtccggca tcaaggaccc
      781 caccatcacc aggatggtga tgggggtgtc gaactgggtg tgcctaaacc ttaaaacacg
      841 gcagagggtc caggccacgg ccacgtgcac catccagtcc ggcattttag cgcctccgga
      901 acaggaacct tctagagtcc aggtaccagt aacggtggaa aatgaagatc atgaataaga
      961 ggaccaggaa ggatctgatg atcaggaagt gatatttcca catctggtta acgtagatct
     1021 tctcaacact aatgatgctg tactccggcc ccagcacccc caagaggtcc acccggtcac
     1081 caatcgccac tggagtccca tttatctcca cgctcaccac ctggccgtgg tagatctgct
     1141 ggatctggta gccgccagga tagttttcca ccacggtgcc gtagacccgt accacttcac
     1201 cctccgggta ttcggctaaa atagcccggt aggagggatg ctccaggtga ttctcgtatt
     1261 cagcagcata gtaggcacat aaggccacga tgactcccag tacaaggaaa cccaccagga
     1321 tccggcttcg gggtgattcg aggttcatct tggtgtatct ccataatatt aatgcttaaa
     1381 tttagggatc cgcaccataa ttaatctatg cagccaccat gacaattaca ccatatcaaa
     1441 tggatgattg gaatagaatc tgctaatcca aactggttta aaaaaagtgt ttatggtgta
     1501 gatctactct atatgtgtaa ttaacaattc attgggataa taaatgatcc atgaccctgt
     1561 aatttttttt taacttattc catgggtata gggggtaaca tggctctgca gagagcagct
     1621 tatcctatgg ccacatgggt agggagaagt agaaggtact cccatccccc tctgactcgg
     1681 cccagatctc acccccgtgc tggtgcacga tcctctgggc tatggccagg cccataccgg
     1741 tgccggggta ctggtcctgt ctgtgcaggc ggctgaagat ggtgaagatg gattccaggt
     1801 gctgggggtc tatgccgatg ccgttgtcct gtacactgaa gaccacttgg tccccctcct
     1861 cccgggcctt gactctgatc cagggtgctt cctccccccg gtacttgatg gagttgctta
     1921 tgaggttctg gaagatgcgc accatctggg tcttatcagc ctccaggata ggcaggtcct
     1981 ggcagtcaat cctggcctgg ttctcccgga tgaggacatt caggttgaag ctaacctcct
     2041 ccagcacctc ctggaagtcc accgggctgc gctgtatctc ctggttggcg atgcgactgt
     2101 aatccaagag ggagttgatc atctcgtcca gccttttagc cccgtccacg gcgtagtaga
     2161 tgaagtcctg ggcgtcctgg tccagctggt cctggtagcg cctctccagt aactggagga
     2221 agttgctgat catccggagc ggctcccgca ggtcatggct ggcgatgtag gcgaactgtt
     2281 gcaggtcgga gttggaacgg gccaactctt cggtgcggag ccgaacctcc ttttccaggc
     2341 tctcccagta gatccgtaac tcctcctcgg cctgtttacg ttcagtgatg tcacgggccg
     2401 cggcgaagat acccaccacc tccccatcct cgtcatggta aaccgaggcg ttgtacagta
     2461 ccggggtgat gctcccatcc tggtgcttga tctccagggg atagtcctta acctccccct
     2521 cctggaagac cctctggtag cccctctctg catcctcggg ttgggtgaag taatcggcaa
     2581 agtcggtgcc gatgagttcc tcccgggagc gtcccgtggt cttctcggtg gcccggttca
     2641 catcggtgat cttaccatca gcgcctatgg tgaccagggg gtccagggca gtctcaatta
     2701 ggct
//
LOCUS       DAOQVW010000191         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_44892_length_2702_cov_3.024556, whole genome shotgun sequence.
ACCESSION   DAOQVW010000191 DAOQVW010000000
VERSION     DAOQVW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44892_length_2702_cov_3.024556"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PKK85_04900"
     CDS             complement(<1..1000)
                     /locus_tag="PKK85_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HNS25390.1"
                     /translation="MKVQSEINIGLVGHVDHGKTTLTKALSGIWTDTHSEETKRGISI
                     RLGYADITFRRCMECPIPQCYTTSLVCEHCGSETQTIRKVSFVDSPGHETLMATMLSG
                     AAIMDGAILVIAANEPCPQPQTKEHLMALDVIGVTEVIVVQNKIDIVSRERAMESYQE
                     IKEFVKGTCAEDAPIIPVSAQQGANIDILIETIHHQIRTPRRSLRKAPRLHVARSFDI
                     NRPGCTPENIQGGVIGGSLIQGKLKLGDEIEVKPGIQIKNKGKMEWMSLISNITGLVA
                     GGESVEEVGPGGLIGVGTNLDPSLTKADSLSGSVAGRPGTLPDILHQFAMKTHLLER"
     gene            complement(1022..1402)
                     /locus_tag="PKK85_04905"
     CDS             complement(1022..1402)
                     /locus_tag="PKK85_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HNS25391.1"
                     /translation="MAFKLVVSEGKNSHQMEVEAALSKKLIGLKIGEEFDASMVGLEG
                     YQLRITGGSDKNGFPMKKDVDGPRRIKSLLSGGVGFKSTRKGQRRRKTVRGNTLSDDI
                     VQVNTVVVKKGEKNLTQLMEGSEE"
     gene            complement(1481..>2702)
                     /gene="infB"
                     /locus_tag="PKK85_04910"
     CDS             complement(1481..>2702)
                     /gene="infB"
                     /locus_tag="PKK85_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNS25392.1"
                     /translation="RDFARQVSIIPISAKTGEGIAELLTMLLGLAQQYLQEQLQIETH
                     APAKGTILEVKEEVGLGTTIDAVIYDGVLKKDDKIALTTRDDVLVTKIRSLLKPNPLE
                     EIRDAKKRFQKVDEVVAAAGIKIVAPNIENVVSGSPLRVARGDLEEVKENILQEMEKI
                     KVQTDEVGVVAKADTLGSLEALLNILKDMEIPIRSADIGDVSRRDVIEASIVKDEEPL
                     YGVIIAFNVKILPSATGELKNREVKLFSADVIYQLTEEYQEWLDAAEKRRKEEWLDAI
                     IRPAKIRIIPKLIFRQSKPAIAGVEILSGTIKKGYGLISQDGVRVGSVESMQDKGDNL
                     IAASKGVKVAMAIKDGVFGRNLDEGDELYVDIPENHYQILEREFKGKLTEDELQLLDE
                     IVQLKRKADPNWGM"
BASE COUNT          535 a          746 c          634 g          787 t
ORIGIN      
        1 cccgttccag tagatgggtt ttcatggcga actggtgcag gatgtccggt aaagttccgg
       61 gcctaccagc cacggagccg gagagggagt cggccttggt aagggatgga tccaggttgg
      121 tccccactcc aatgaggcca cccggcccta cttcctccac tgattcccca ccggccacca
      181 gtccggtgat gttggatatt aggctcatcc attccatttt acccttattt tttatctgaa
      241 ttcccggttt cacctcaatc tcatccccca acttcaactt cccctggatt agggatccgc
      301 caatcactcc gccctggata ttttccgggg tgcagcctgg acggtttata tcaaaggacc
      361 gggccacgtg taggcgggga gctttccgga gtgatctgcg gggggttcta atctggtgat
      421 gtatggtttc aattagtatg tcgatgttgg cgccctgctg ggcagagact ggtatgatgg
      481 gtgcgtcttc ggcacaggta cccttcacga attccttgat ctcctggtaa ctctccatgg
      541 ccctctcccg ggagacgatg tcgatcttgt tctgcaccac gatgacctcg gtgactccaa
      601 tgacatccag ggccatgaga tgctccttgg tctggggctg gggacagggc tcgttggcag
      661 cgatgaccag gatggctcca tccattatag ctgctccgga gagcatggtg gccatcaggg
      721 tttcgtgccc tggggaatcc acaaaagaaa ccttccggat ggtctgggtt tcacttccac
      781 agtgttcaca aaccagggag gtggtgtaac actggggtat gggacactcc atgcaccggc
      841 ggaaggtgat gtccgcatag cccagtcgta tagatatgcc ccttttagtt tcttcactgt
      901 gagtatctgt ccatattcca gatagggcct tggttagggt ggttttccca tggtccacgt
      961 gtcctactaa cccgatattg atttcagact gtactttcac ggactacacc tttttttatc
     1021 tttattcttc gctaccttcc atcagttggg taaggttctt ttcacccttc ttcaccacaa
     1081 cggtgttcac ctggacgata tcatcagaca gggtgttccc acggacggtt ttcctccttc
     1141 tctgaccttt acgggtggat ttaaacccaa ctccgcctga aaggagactt tttatccgtc
     1201 gcggtccatc cacatccttt ttcatgggga aaccgttttt atcactccct ccagttatgc
     1261 gcagttggta gccttccaag cccaccatag aagcgtcaaa ttcttcacca atctttaatc
     1321 ctatgagttt cttggataaa gcagcctcca cttccatttg atgactattt ttaccttctg
     1381 aaacaaccag tttaaatgcc aatctatctc ctccatctta actctgctat atatattata
     1441 ctatttatcg gtttttatgc ttttattgaa gaaatctaac tcacattccc cagttaggat
     1501 cagccttcct ctttaactgg acgatctcat ccagaagttg gagttcatcc tcggtgagct
     1561 tacccttaaa ctctctctcc aggatctggt aatgattctc agggatgtcc acatagagct
     1621 catccccttc atccaggttg cggccaaaca ctccgtcttt aatggccatg gccaccttaa
     1681 cgcccttgga agcagcgatc aggttatctc ccttgtcctg catgctttcc acactaccca
     1741 cccttactcc gtcttggctt atcaagccgt atcccttctt gatggtgccg gagaggattt
     1801 ccaccccggc gatggcgggt ttgctctggc ggaatatgag ttttggtatg atccggatct
     1861 tggctggtct gattatggca tccagccatt cctccttgcg ccgtttttct gcggcatcca
     1921 gccattcctg gtattcctcg gtaagctggt agatgacatc tgcagagaag agtttcactt
     1981 ccctgttctt gagttcccct gtagccgaag gaaggatctt gacgttgaag gcgatgatca
     2041 ccccgtagag gggttcttca tccttaacta tggaggcctc aatgacatcc ctacgggaaa
     2101 catcaccaat gtctgcagag cggattggta tctccatgtc cttgaggatg ttcaggaggg
     2161 cttccaggga tcccagggta tctgctttgg ccaccacgcc cacttcgtct gtctggacct
     2221 ttatcttttc catctcctgg agtatgtttt ctttaacctc ctccaggtct ccgcgggcca
     2281 cccgcaaagg agagccagat acaacgttct cgatgttggg ggccacgatc ttgattccag
     2341 ccgcagccac cacttcatcc accttctgga agcgtttttt agcgtctctt atctcctcca
     2401 gggggttggg ttttagcagc gatcttattt tggtgaccag aacatcgtcc ctggtggtca
     2461 gggcgatttt atcatccttt ttaagcactc catcgtatat gacggcgtcg atggtggttc
     2521 ccagtcccac ttcttccttt acttccagga tagttccctt agccggggca tgggtttcta
     2581 tctgcaactg ctcctggagg tactgctggg ccaatcccag gagcatggtt aaaagttcag
     2641 cgattccctc accagtcttg gcactgatgg ggatgatgct tacctgcctg gcgaagtccc
     2701 tc
//
LOCUS       DAOQVW010000192         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_45086_length_2694_cov_3.416446, whole genome shotgun sequence.
ACCESSION   DAOQVW010000192 DAOQVW010000000
VERSION     DAOQVW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45086_length_2694_cov_3.416446"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /gene="sfsA"
                     /locus_tag="PKK85_04915"
     CDS             complement(<1..583)
                     /gene="sfsA"
                     /locus_tag="PKK85_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HNS25393.1"
                     /translation="MKIGNIIKGTYQERTNRFTVVFKYGEREEKAHLRDPGRLRELLL
                     PDADLLLRPAQNLAKRKTKFDVIAVRSEDIWVLINSGFHSDLAQELINSRLISEFKGY
                     DIVKREYSYGKSRIDFFLKHGQSELLLEVKGCTLVEEGHARFPDAPTERGKRHVEELL
                     RAKIQGLSAAVLFIIPREDAMIFSPNWEMDPQFS"
     gene            complement(580..1395)
                     /locus_tag="PKK85_04920"
     CDS             complement(580..1395)
                     /locus_tag="PKK85_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25394.1"
                     /translation="MDALFFTSNPDLIGKIIFTSYNKIMVMKKRSIGWFLISILILIT
                     GLATVNTLELSANTTSNEVPQIKLPEVVKILGVDDRGYVVRRGPYGNPNATITIAYIL
                     GVHPLEYESHRAIRTAVMERKNSLSYKYYVYDVNVLRDRYDYDLGRTYGQELARDYVV
                     PDIVNQEFELVIDVHSNRGNYSEKVFITSLVSDKDSDNASLQIVSKLPWLKRYRPPAE
                     PGPTSGPYVSIPLIRSGTPTLVYETYRYDQYDLVLWQAFNLVDAVDEIRWDFK"
     gene            1386..2459
                     /gene="cfbD"
                     /locus_tag="PKK85_04925"
     CDS             1386..2459
                     /gene="cfbD"
                     /locus_tag="PKK85_04925"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HNS25395.1"
                     /translation="MHPRPSPIAASLYTLRDLNADVVILHGPHGCCFRTGRLLENDGV
                     RVLTTAMSENDFIFGAAEKLEETLKKADEMFSPQLVGVVGTCASMIIGEDLKEAVEKV
                     NIPATVLSVESHGGFGEGDNTEGAIIVLESAQREGIISQEEVSRQVRMLKMATEIEKT
                     RGMAQGGYIAPQYGDDKSEVAKILLNAFESGEKISMVLNAKKETSYLFADLFRIPFHQ
                     LYPENPPTIIANLDVDVGLPRIRQHARNIQGELAKEGVEISVITGGLDEYPLTGRKAV
                     EFVKSDDYDVVVVAGVPHALPIEDVSAETVAITDGPRLVEPLKKLGYTRVVTELDAHS
                     KTLGTSKIVPSDFGTIVREILNQ"
     gene            2551..>2694
                     /locus_tag="PKK85_04930"
     CDS             2551..>2694
                     /locus_tag="PKK85_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNS25396.1"
                     /translation="MSKTVGMILCGGFGKRLRPLTERVPKPLIEIKENYTILDKQLFD
                     FKNS"
BASE COUNT          769 a          598 c          612 g          715 t
ORIGIN      
        1 gagaaaattg tggatccatc tcccagttcg gtgagaaaat catggcatcc tctcggggga
       61 tgatgaaaag aactgcagca cttaatcctt ggattttagc ccttaaaagt tcttcaacgt
      121 gccttttacc ccgttcggtg ggggcatcag ggaacctggc gtggccttcc tccaccagtg
      181 tgcagccttt aacctccagg agcagttcac tctgaccatg ttttaaaaaa aaatcaatcc
      241 tacttttacc atagctatat tcccttttta caatatcata tcccttaaat tctgaaatta
      301 acctagaatt tatgagttcc tgggccaggt cactgtggaa acccgagttt ataagcaccc
      361 agatatcctc actcctgact gcgatgacat cgaatttggt tttccgctta gcaaggtttt
      421 gcgccgggcg cagcaataaa tccgcatctg gcagtaacag ttcccgtaat ctgcccggat
      481 cccgaagatg ggccttttct tcacgttccc catatttgaa tactactgtg aagcggttgg
      541 tcctttcctg ataggtgccc ttaattatgt tcccaatttt catttaaaat cccatcttat
      601 ctcatccact gcatcaacca gattaaaggc ctgccaaagc accaagtcat attgatcata
      661 gcggtaggtt tcataaacca gggtgggagt gcctgatctg atgaggggta tactgacgta
      721 aggtccgctg gtgggtcctg gctcagcagg gggtctgtat ctctttagcc atggtagttt
      781 agagactatc tgtagggagg cgttgtctga atccttatca ctaaccaggg aggtgatgaa
      841 taccttttct gaataatttc cacggtttga atgtacatca ataaccagtt caaattcctg
      901 gttaacaata tcgggcacca cataatccct ggctaattct tggccgtaag ttcttcccag
      961 atcataatca tagcgatcac ggaggacatt cacatcgtag acatagtatt tataggataa
     1021 agagtttttt ctttccatca cagccgtcct gatagcccga tgagactcat attccagggg
     1081 atgcaccccc aggatgtaag ctatagttat tgtggcgttc gggtttccgt aggggcctct
     1141 ccttaccacg tagccacgat catccacccc caggattttt acaacctccg ggagcttaat
     1201 ctggggaact tcattagaag tagtgttagc acttaattct agggtattga cagtggctaa
     1261 accagttatg agaatcaaaa tgcttattag aaaccaccct atacttcgtt tcttcatcac
     1321 cattatttta ttataagagg tgaatataat ctttccaatg agatcaggat tagaggtgaa
     1381 aaatagtgca tccacgaccc agccccatag cagcatcact ttacacgctc cgagacctta
     1441 atgccgacgt agtaatcctt catgggccac acggctgctg ttttcgtacc ggaagacttt
     1501 tagaaaatga tggagttcga gttttaacca cagccatgtc agaaaatgat ttcatctttg
     1561 gagcagcgga aaaactggag gaaaccctga agaaagccga cgaaatgttc tcgccccaac
     1621 tggttggagt agtgggtacc tgtgccagta tgataatagg ggaagacctc aaggaagccg
     1681 tggaaaaggt caacattccc gccacggtgc tgagtgtaga atctcatgga ggttttggag
     1741 aaggagataa cacagaaggg gccatcatcg tattggaatc agcccagagg gaagggatca
     1801 taagccagga agaggtgtca aggcaggtgc gtatgctaaa aatggccact gaaatagaga
     1861 agactagagg gatggctcaa ggcggttata tagcccccca atacggagat gataaatcag
     1921 aagttgccaa aatactgtta aatgcatttg aatcgggtga aaaaatttcc atggtgctta
     1981 acgccaagaa agagacttct tatctctttg cagacttgtt cagaattccc ttccaccaac
     2041 tgtaccccga aaaccccccc accataattg ccaacttgga tgtggatgtg ggattgcccc
     2101 gaataagaca acacgcccgg aacatacagg gagagttagc caaggaaggt gtggaaatat
     2161 cagttataac tggtggcttg gatgagtacc ccctcactgg gcgaaaagca gtggaatttg
     2221 tgaaaagtga tgattacgat gtggtggttg tggcgggtgt tccccatgcc ttgcccatcg
     2281 aggatgtatc agctgaaaca gtggcaatca cggatggtcc cagactggtg gagccactga
     2341 agaaattagg atacacccgg gtggttactg aattggatgc ccattcaaag acattgggaa
     2401 ccagtaaaat cgttccatct gactttggaa ctattgtaag ggagatttta aaccagtaaa
     2461 gaccttcacc cactactaac tttttatagt tgaattttaa aaagtatagc ccagagatgc
     2521 taataatttc tttggtttaa aagtgatact atgagcaaaa cagtgggaat gatactctgc
     2581 ggaggttttg gaaagcgtct acgacccctc accgaaaggg ttcctaaacc cctcatagaa
     2641 ataaaggaaa attacaccat cctggacaag caactttttg actttaaaaa ctca
//
LOCUS       DAOQVW010000193         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_45214_length_2690_cov_2.945351, whole genome shotgun sequence.
ACCESSION   DAOQVW010000193 DAOQVW010000000
VERSION     DAOQVW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45214_length_2690_cov_2.945351"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKK85_04935"
     CDS             complement(<1..276)
                     /locus_tag="PKK85_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNS25397.1"
                     /translation="MEDHELLSAIVGEYFPILEFVYDKRAPYFLVGDYSTAKFSALVE
                     ELDQVGYVPFINPDGLYYRIRLAHKPEEINSRIYINLILFLATLGTTL"
     gene            complement(331..528)
                     /locus_tag="PKK85_04940"
     CDS             complement(331..528)
                     /locus_tag="PKK85_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNS25398.1"
                     /translation="MNITFIMGEEEKIMETGGGSIKNLLQELEIPLETVVVKKNQQIV
                     IEEEPLEEGDTIEVIRVIYGG"
     gene            complement(616..1524)
                     /locus_tag="PKK85_04945"
     CDS             complement(616..1524)
                     /locus_tag="PKK85_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HNS25399.1"
                     /translation="MKCDSCGSGPVIIHRKHSGQVLCKDCFIQRMQKKVLKDIRKYKL
                     VRKGDKILLGLSGGKDSVMTMDILSNLQERGIVELVAVTIDEGIAGYRAEGVEIATEI
                     AREMNIQHKVVSFNEYLGVTLDEMMKNPHSGACTYCGVFRRWILNRVAREVGATKIAT
                     GHNLDDEVQSILMNYLEGNLDNLTRIGVKSQSNDPRFTVKIKPLREVPEKEVALYVLA
                     RELPVHLAECPYSGDSFRAEVGHFLKEISLKHPTIMYSTLRGFDRIKPVLKKEYSRKS
                     PHTMCEVCGEPAATPLCKACSFLKMM"
     gene            complement(1575..>2690)
                     /locus_tag="PKK85_04950"
     CDS             complement(1575..>2690)
                     /locus_tag="PKK85_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HNS25400.1"
                     /translation="LPVGEDFPMRVLAEIANAPLLSPENLLKRAEYFVKSGADLVDLG
                     MLAGEDLKSRIPPMVNLLKEKLDVPISIDTLNPAEIEVAVESGVDLVLSLDHGNYQEL
                     VPLLKERKVPAVLLPTNYSQNWVPHTWEERVTSLNGLIKKCKGLEVIADPVLDPVNSE
                     SIMDSFLACRQLKRENQVPIFFGVGNVTELMDVDSTGANTLLSGIGMELGVSVLFTPE
                     ESGKTVGSVKELAVSSQMMFLAKKRGSIPKDLGINLVVFKDKRRGETLEDAVDVPEVE
                     GQPDYTFKQDSAGSFKITVSEGLIRAVHYQKSEPHMAIVGTEAKALYDEIIKRNLVSK
                     LEHAAYLGSELQKAEESLKFGKHYTQDFPLFKKFMEI"
BASE COUNT          635 a          638 c          568 g          849 t
ORIGIN      
        1 cagggtggtt cccagggtgg ctaggaatag tatcaggttg atatatatcc gggagttaat
       61 ttcctctggt ttatgggcca gtcggatcct gtaatacaat ccatcaggat taatgaaggg
      121 cacgtaaccc acttggtcca gttcttcaac cagagcggaa aatttagcag tactataatc
      181 cccaaccagg aagtagggtg ctctcttatc atacacaaat tctaggatgg ggaaatattc
      241 cccaacaata gcagatagaa gctcgtggtc ttccactagt tcacgtccct gttacaataa
      301 tcagtttcac agccatgagg atggggatag ttaaccccca tatataaccc ttataacctc
      361 aatggtgtct ccttcttcta gaggttcttc ttcaatgacg atctgctggt tttttttaac
      421 cacaactgtt tccaggggta tttccagctc ctggaggagg ttcttaatgc ttcccccccc
      481 tgtttccatg attttctctt cttctcccat tataaaggtt atgttcatct aatcacatcg
      541 ggtctggtct gcagatattt tttttggtga gtgaagttaa ctgtatctat ttaatgatct
      601 atttagattt aatcgttaca tcattttaag gaagctgcag gccttacata gtggagtggc
      661 tgctggttca ccacatactt cacacatggt atgtggggat tttctggaat attctttctt
      721 cagcactggt tttatgcggt cgaatccccg gagggtggag tacatgatgg tgggatgttt
      781 cagggatatc tcctttaaaa agtgccctac ttccgcgcgg aaagaatcac cggagtaggg
      841 gcattctgcc aggtgaaccg ggagctccct ggccagtaca tagagtgcta cttccttctc
      901 tgggacctcc cggaggggtt tgatttttac ggtgaatcgg ggatcattgg actgggattt
      961 tacaccgatc cgtgttaaat tatccaggtt tccttccagg taattcatga ggatggattg
     1021 gacctcatca tccaggttgt gaccagtggc tattttagtg gcacctactt cccgtgcaac
     1081 tctgttgagt atccatcttc tgaatactcc acagtaagtg caggctccgc tatgtgggtt
     1141 tttcatcatt tcatccagag taacccctaa atattcattg aaggaaacta ctttatgttg
     1201 gatgttcatt tccctggcaa tttcagtggc aatttccaca ccttctgccc ggtagccagc
     1261 tattccctca tcgatggtca cagccaccaa ctccacaata cccctttcct ggagattact
     1321 cagaatatcc atagtcataa cactgtcttt tccaccagat aaacctaaaa ggatcttatc
     1381 tccctttctg actagtttgt attttctgat atcctttaaa accttttttt gcattctttg
     1441 gatgaagcaa tctttacaca gtacttgccc ggagtgttta cggtgaataa ttaccggacc
     1501 cgagccacaa gaatcacatt tcatggtaaa atcctattta ataccttatt tatcataata
     1561 attgcttcag gatcctagat ttccataaat tttttgaata aggggaagtc ctgagtgtaa
     1621 tgttttccaa atttcaatga ctcctctgcc ttttgaagtt ctgatcccag gtatgcggca
     1681 tgttccaatt tgcttaccag gtttcttttg attatctcat cgtagagggc cttggcttcg
     1741 gtgccaacta tggccatgtg gggttcactc ttctggtagt gcactgccct tatcaatccc
     1801 tcggagactg taattttgaa gctgccagca gaatcctgtt tgaaagtgta atctggttgt
     1861 ccttctactt cgggaacgtc aacagcatcc tccagggttt cacctcgtct tttatccttg
     1921 aagaccacca gattaattcc caagtccttg ggtatggaac ctcgtttctt ggccagaaac
     1981 atcatttgag atgatactgc cagttccttc acactaccca ccgtttttcc actttcttcc
     2041 ggtgtgaaga gtacactcac tcccagctcc attccaatac ctgatagtaa agtgtttgct
     2101 ccggtagagt ccacatccat gagttcggtg acgttgccta ccccgaaaaa aatgggaact
     2161 tggttttctc ttttcaactg gcggcaggcc aagaaggagt ccatgatgct ttcactgtta
     2221 actggatcca gcactggatc agctatgact tccaggcctt tacatttttt tataaggcca
     2281 ttcagggaag ttactcgttc ctcccaggta tggggtaccc agttttgact gtaattagtg
     2341 ggtaatagta ctgctggtac tttcctttct ttaagcaggg gtaccagttc ctggtagttg
     2401 ccgtggtcca ggcttaaaac cagatccacc cctgattcaa ctgccacttc aatctccgcc
     2461 gggtttaggg tatcgatact gatgggaacg tcaagttttt cctttaaaag atttaccatg
     2521 ggtggtattc tgcttttcag gtcctctcca gctagcattc ccagatccac cagatcagca
     2581 ccggatttaa cgaagtactc cgcccttttc agtaagtttt ccgggcttag aaggggggcg
     2641 ttggcaattt cagccaggac tctcatgggg aagtcctccc ccacagggag
//
LOCUS       DAOQVW010000194         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_45903_length_2664_cov_4.526255, whole genome shotgun sequence.
ACCESSION   DAOQVW010000194 DAOQVW010000000
VERSION     DAOQVW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45903_length_2664_cov_4.526255"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /gene="top6B"
                     /locus_tag="PKK85_04955"
     CDS             <1..1238
                     /gene="top6B"
                     /locus_tag="PKK85_04955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HNS25401.1"
                     /translation="QGLGCSGCVLLSQMTTGKPAKVVSGWYEGEKLKGVEMVFKMDVK
                     TNQGLILSRKEVEVDSTGVKVELHFKDVSYSLSEQGAYEYIRRTMIANPHCKITFRDP
                     TGHKYIFDRADKGIPPLPKEVLPHPKGVTADDLIFMAKHTDKRRFRSLLTSNLSRMST
                     KRVKEIEEITGIDLNKRPKDMKWEEAETIVETFAKMDFMAPPTSGLIPIGKEQIDKGM
                     REILTPEFISSITRKPKTFRGGISFVVEAGISYGGDSGRMVGEQRKAEIMRFANRVPL
                     TFDQGSCAITEALKSIDWKRYGVRDLDNAPITVFVNIVSTNVPYLSTGKQSVAPEPEI
                     LHEVRQATMKVARKMQKYIRAKRAAKEEALRSKVFESLVPVIIREAALLAEQDVPEYD
                     AVLAKVTRRTKYEGVVEDE"
     gene            1231..2286
                     /locus_tag="PKK85_04960"
     CDS             1231..2286
                     /locus_tag="PKK85_04960"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNS25402.1"
                     /translation="MNKKDITVNKLKSLGETILEDVGKNKVPTIKVPSRGTSNIVYDE
                     KKRYYVLGDRYGKRSLGNVKQISKMAQMVYVANFCKDLIRRGKTATLREMYYVSEGWD
                     VDFGDQQESNIVGEDLEVTLGMTREDLGLMPEEDGASVYGNIVIQDDDVEINALKSGK
                     SGYTISPTIDEVEFVDHDVKRVIAVETMGMFHRMVQEEAYKKFDTLIVGLKGQAARAT
                     RRFLKRVNEELNLPVYICNDGDPWGFHIAMVIISGSAKLAHVNHQLATPNAKFLGVTA
                     SDIINYDLPTDPLKDIDVLRLKELSKDPRYRDDAWQMEIKKMLKIGKKAEQQSFSKYG
                     LEYVVDTYLPEKLEQME"
     gene            2548..>2664
                     /locus_tag="PKK85_04965"
     CDS             2548..>2664
                     /locus_tag="PKK85_04965"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032973.1"
                     /note="catalyzes the formation of 3-phospho-D-glycerol
                     phosphate from D-glyceraldehyde 3-phosphate in glycolysis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNS25403.1"
                     /translation="MKSIGINGYGTIGKRVADAVACQDDMKIVGVTKRTPDFE"
BASE COUNT          844 a          560 c          623 g          637 t
ORIGIN      
        1 agcaaggcct gggatgcagt ggctgtgtgc ttttatccca gatgaccacc ggaaaaccag
       61 ccaaggtagt ttctggatgg tacgagggag aaaagcttaa aggagttgag atggtcttca
      121 aaatggatgt taagaccaac cagggcctga tattatccag aaaggaagtg gaagttgatt
      181 caaccggggt gaaggtggaa ctacatttta aagatgtctc ctactcttta agcgaacagg
      241 gcgcttacga atacattcgc agaaccatga tcgccaaccc ccactgtaaa atcaccttcc
      301 gggaccccac cggacacaag tacatcttcg accgagctga taaaggcata cctcccctcc
      361 ccaaagaggt tttaccccac cctaaggggg ttacagctga tgatttgata ttcatggcta
      421 aacatacaga taaacgacgc ttccgcagtt tactcaccag taacctgtcc cgcatgtcca
      481 ccaaacgggt gaaggaaata gaagagataa ctggaattga cctaaacaag aggcccaaag
      541 acatgaagtg ggaggaagca gagaccattg tggaaacctt cgccaaaatg gacttcatgg
      601 ctccgcctac ctccggtttg atccccattg gcaaggaaca gatcgataag ggtatgaggg
      661 agatattaac tcctgaattc atttcttcca tcaccagaaa accaaaaacc ttcaggggtg
      721 ggatttcatt tgtggtggag gcgggtattt cttacggtgg agattcagga agaatggtgg
      781 gagagcagag aaaagctgaa ataatgcgtt ttgctaatcg agtgccttta acatttgacc
      841 aaggaagctg cgccataacc gaagccctta aaagtataga ttggaagcgt tacggtgtta
      901 gagacctgga taacgcccct ataacagttt ttgtgaacat agtatccacc aacgtgccct
      961 acctatccac cggtaagcag agcgtggcac ctgagcccga aattctccat gaagtccgcc
     1021 aagctaccat gaaagtagcc cgtaagatgc aaaaatacat ccgggccaaa agggcggcga
     1081 aggaagaagc tctccgttcc aaggtatttg aaagcttggt tccggtaata atccgtgaag
     1141 cagctcttct tgcggaacag gatgttcctg aatatgatgc tgtgctagct aaagttacac
     1201 gcagaaccaa atatgaaggc gtggttgaag atgaataaga aagatatcac tgttaacaaa
     1261 ctcaaaagtc ttggtgagac aatactagaa gatgttggaa aaaacaaagt ccccaccatc
     1321 aaagttccat caagaggaac atctaacata gtttacgacg aaaaaaagcg ctactacgta
     1381 cttggagaca gatacgggaa aagatccctg ggtaatgtta aacagataag taaaatggcc
     1441 cagatggttt acgtagccaa tttctgcaag gacctgattc gaaggggaaa aacagctact
     1501 ttaagggaga tgtactacgt ttctgaagga tgggacgtgg attttggaga ccaacaggag
     1561 tccaacattg taggtgaaga cttggaagtt accctgggaa tgacccgtga agatctgggc
     1621 ctaatgccag aagaagatgg tgcttcggtc tatggaaaca tcgtcatcca agatgatgac
     1681 gtggagatta acgcccttaa atctggtaaa tcaggttaca ccatttcacc caccattgat
     1741 gaagttgaat ttgtggatca cgatgttaaa cgtgttatag ccgtggaaac catgggtatg
     1801 ttccaccgga tggtccaaga agaggcatat aaaaaatttg acactttgat tgtcggttta
     1861 aagggacagg ctgctcgcgc tacccgccgt ttccttaaaa gagtgaatga agaacttaac
     1921 ttgccagtgt acatctgtaa cgacggagac ccctggggat ttcacatcgc catggttatc
     1981 ataagcggca gtgccaaact agcccacgta aaccaccagt tagccacgcc caatgctaaa
     2041 ttcctgggtg ttactgcctc ggatatcatt aactacgatc ttcccaccga tccccttaaa
     2101 gatattgatg ttttaaggct aaaggaactt tccaaggacc cccgctaccg ggatgatgct
     2161 tggcaaatgg agatcaagaa gatgctcaag atcggtaaaa aggcagaaca acagtctttc
     2221 tcgaagtatg gattggaata tgttgtcgac acatatctac ctgaaaaatt agagcagatg
     2281 gaataaacaa attccttcca tcttttatca gccccaaaat attcaagact ctttcatttc
     2341 ttttttaaat accttaaatc cagaggttgt gaattaccac cttaccctac cgataaaaat
     2401 caaaaaattt attataactt gggaacataa agattcataa agagtttttc tgtcagatag
     2461 gctttctggg agttttattt ttgaaataat ggcctaaacc ttttaaactt ccaggatccc
     2521 aggaaaagta aggcggatgt gtaaatgatg aaatcaatcg gtataaacgg atacggaacc
     2581 ataggtaaaa gggtggcaga tgctgttgcc tgccaggatg acatgaagat cgtcggggtg
     2641 acaaagagaa cccctgactt tgaa
//
LOCUS       DAOQVW010000195         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_46035_length_2659_cov_2.966206, whole genome shotgun sequence.
ACCESSION   DAOQVW010000195 DAOQVW010000000
VERSION     DAOQVW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46035_length_2659_cov_2.966206"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKK85_04970"
     CDS             complement(<1..193)
                     /locus_tag="PKK85_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNS25404.1"
                     /translation="MTRYRLLIPLLIILLGVIWYSNSQLPFSSSAYLPGPQLTAADRV
                     MIVAVHPDDETIATGGLINY"
     gene            complement(261..1232)
                     /locus_tag="PKK85_04975"
     CDS             complement(261..1232)
                     /locus_tag="PKK85_04975"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNS25405.1"
                     /translation="MVKKKILNSLNQVSGEYQEEEELATQAGISVDELRDEIQAMVGD
                     GFVIESSEEGYKLVEIPNRLLPYQIQRGLDTSYIGHDIRLFSEVDSTNQVAKDLAEEG
                     ASEGTIVIAEVQRRGRGRHGKRWLSPEGGVWMTIILRPEIPPAKAPLLTLLTGVAVAE
                     TLQTECGLDVGIKWPNDILIGDKKVCGILTEAEAGKGNLEYVVVGIGIDLNVDLDDFP
                     PELKYGATTLQKELQKEIYGVEVVQRFLKNFEELYEQFKDGQFPEILSQWRRLSKTIG
                     RYVEVHKKGRTVYGEAVGITKDGVLILELDDGTLKKVISGECIHLPH"
     gene            complement(1249..>2659)
                     /locus_tag="PKK85_04980"
     CDS             complement(1249..>2659)
                     /locus_tag="PKK85_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNS25406.1"
                     /translation="VKSVAVYSEADNNSLFAKYADEAHYIGGPSPAESYLNIPHIIDA
                     ALKSGAEAIHPGYGFLAENPQFGEECEKNGLKLIGPPGSVIRDMGSKIISRKLMEKAG
                     VPVVPGTSRGVTDPDEAQEIAGAIGYPVIVKASAGGGGIGMRTVYEEDELLRALESTQ
                     SVAGSAFGDSTVFIEKYIEEPRHIEFQILADEGGHTIHVADRECSIQRRHQKLIEESP
                     SPIMTPELREQMGSAAVRAAASIGYSNAGTVEFLYSDGEFYFLEMNTRIQVEHPITEM
                     VTGVDLVQEQLKVAAGQELNWEQEDIMIRGHAIECRINAEDPLMDFAPNPGKITGYRS
                     PGGLGVRVDSGVYMNYTIPPYYDSMISKLISWGNNRREAINRMRRALSEYVILGVKTT
                     IPFHQSMMLSPHFQEAKLHTHFVDEYKKEIMANMEKVIAEDQARVERLRSTFLPSKKV
                     AAVSAAVNSYMAHDVARKK"
BASE COUNT          566 a          738 c          662 g          693 t
ORIGIN      
        1 agtaatttat aagccctccc gtggctatgg tctcatcatc ggggtggact gccacgatca
       61 taacccggtc tgcagctgtt aactgcggac caggaagata ggccgaggaa gaaaatggta
      121 actgggagtt ggagtaccat atgactccta acaggataat taggaggggt atcaataacc
      181 tgtatcgagt catctttaac tccttacttt aaaaaggatg acaaatatta tctacatgtc
      241 ttgttggggc tgggaacgta ttagtgtggt aggtgaatgc attccccgga tatcaccttc
      301 ttcagggtgc catcatccaa ctccagtatc agaacaccat ctttggtaat tcccaccgct
      361 tctccataga ctgtgcgacc cttcttatgg acttccacgt atctacctat ggttttagat
      421 agtctacgcc actgggatag gatttcaggg aactggccat ctttgaactg ttcgtagagt
      481 tcttcaaagt ttttaaggaa tctctgcacc acttccaccc cgtagatctc cttttgcagt
      541 tccttctgta gagtggtggc cccgtatttt agttctgggg ggaaatcatc cagatccacg
      601 ttgaggtcga tacctatacc caccaccaca tactctaggt tccccttacc agcttcggcc
      661 tcggtgagta tgccgcagac ttttttatca ccaatgagta tatcgttggg ccatttaatc
      721 cccacatcca atccacactc tgtctgcagg gtttcagcca cggccacacc agttaacagg
      781 gttaagaggg gagccttggc tggggggatc tccgggcgga ggattatggt catccataca
      841 ccaccctccg gggaaagcca ccttttccca tgacgacccc taccccgcct ttgcacctcg
      901 gcgatgacta tggttccctc ggaggcaccc tcctcggcca ggtccttcgc cacctggttt
      961 gtggaatcca cctcggagaa gaggcgaata tcatggccaa tgtaactggt atccaggccc
     1021 ctctggatct ggtagggtaa cagacggttg ggaatctcca ccagtttata tccttcctcc
     1081 gaagattcta tcacgaaacc atcccctacc atggcctgga tctcatcccg tagttcatcc
     1141 acgcttatcc cggcctgggt ggctagttct tcttcttcct ggtattctcc cgaaacctgg
     1201 ttgaggctgt tgaggatctt ctttttaacc atatcaaatc accactcttc actttttcct
     1261 agccacgtca tgggccatgt aactgttcac cgcagctgaa accgcagcca ccttcttgga
     1321 ggggaggaag gttgatctca atcgctccac acgggcctgg tcctcagcta tcaccttctc
     1381 catgttggcc atgatctcct ttttgtactc atccacgaag tgggtgtgca gcttggcctc
     1441 ctggaagtgg gggctgagca tcatggactg gtggaagggt atggtggtct tcacccccag
     1501 gataacgtac tctgataaag cccggcgcat ccggtttatg gcttctcggc ggttgttacc
     1561 ccaggatatg agtttggaga tcatggagtc gtagtagggg ggtatggtgt agttcatgta
     1621 caccccactg tccacccgca cgcccaatcc acctggagac cggtaaccag ttatcttccc
     1681 agggttaggg gcgaaatcca tcagggggtc ctcggcgttg atacggcact cgatggcatg
     1741 tccccgtatc atgatatcct cctgctccca gttaagttcc tggcctgccg ccactttaag
     1801 ttgctcctgg accagatcca cacctgtcac catctcggtg atgggatgct ccacctggat
     1861 ccgggtgttc atctccagga agtaaaattc accatcagag tagagaaatt ccactgtacc
     1921 ggcgttactg taaccaatgg atgctgctgc cctcaccgca gcggagccca tctgctcccg
     1981 taactcgggg gtcatgatgg gtgagggtga ctcctcaatt aacttctgat gccttctctg
     2041 gatggagcac tcccggtcag ccacgtgtat ggtatggccc ccttcatcgg ccaggatctg
     2101 gaattcgatg tggcggggtt cctcgatgta cttctcgatg aagactgtgg agtctccgaa
     2161 ggctgatcca gccacggatt gggtggattc cagtgccctg agaagttcgt cctcttcgta
     2221 gacggtgcgc atcccaatac cacccccacc agcagaggcc tttacaataa ctggataacc
     2281 aatggcacca gctatttcct gggcttcatc tgggtcggta accccccgac tggttccagg
     2341 gaccaccggg acccctgctt tctccatgag cttccgggag atgatcttgc tgcccatatc
     2401 ccggatgacg gatccaggag ggccgatgag tttcaggccg tttttctcac actcctcacc
     2461 aaactggggg ttttccgcca ggaaaccgta acctggatgt atggcttcgg ctccggactt
     2521 taaagcagca tctatgatgt gtgggatgtt aagataactc tccgcagggg agggtccccc
     2581 gatgtaatgg gcttcatcgg cgtatttggc aaagagggag ttattatcgg cttcggagta
     2641 gacagccaca ctcttgacg
//
LOCUS       DAOQVW010000196         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_46370_length_2647_cov_2.896605, whole genome shotgun sequence.
ACCESSION   DAOQVW010000196 DAOQVW010000000
VERSION     DAOQVW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46370_length_2647_cov_2.896605"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKK85_04985"
     CDS             complement(<1..280)
                     /locus_tag="PKK85_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25407.1"
                     /translation="MCIASENLTSNITALNSSTDDLDITTTDYANQTSNLNQSTTNTT
                     LEEGVTAAGEEQSTPQSDTPTPQYNNAQGIWIHRDDLRNIDVNALVEAG"
     gene            complement(1025..1543)
                     /locus_tag="PKK85_04990"
     CDS             complement(1025..1543)
                     /locus_tag="PKK85_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNS25408.1"
                     /translation="MAKIAVILEDWFEDVECTEPAAAFKEAGHDLVLVGLQAGKVVSG
                     KRHTVQATIERSLEMASVDEFDALLIPGGYSPDRLRAHDVAVRFVKEFIDSGKPVFSI
                     CHAPQLLITADVLRDRKVTGYTSIIQDIKNAGAEFIDAEVVVDGNLVSSRNPGDIPVF
                     IRESLRLLDEGG"
     gene            1776..1997
                     /locus_tag="PKK85_04995"
     CDS             1776..1997
                     /locus_tag="PKK85_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNS25409.1"
                     /translation="MFRDSYSNDRDSSAPIKEGQEYDVKIEDTGRDGDGIARIEGFVV
                     FVSGAKVGDEVKIRVNSTRRNFAFAEVVE"
     gene            complement(2166..>2647)
                     /locus_tag="PKK85_05000"
     CDS             complement(2166..>2647)
                     /locus_tag="PKK85_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS25410.1"
                     /translation="GDSDHAGNCAFLRDKYEAPVAMHQADSGMVTQGDMLYNRRGNLL
                     SRIISPFMGLGKRHRFIPDFYVEDAYHLLPYGVEARVVHLPGHSAGSIGVLTSEGHLF
                     CGDLFTNTKEPALGAIMDDEAAARASVERLKEMDIKYVYPGHGDPFPWKLLEEGIGL"
BASE COUNT          770 a          573 c          546 g          758 t
ORIGIN      
        1 ctccggcctc aaccagggcg ttgacatcta tattcctaag atcatcacgg tgaatccaga
       61 tcccctgagc gttgttgtac tgcggtgtag gagtatcact ttgaggggta gattgttcct
      121 ctccagctgc cgttacaccc tcttcaaggg tggtgttggt cgtgctctga ttgaggttac
      181 ttgtttgatt cgcgtaatca gtggtggtta tgtcaaggtc atctgtagag ctgttaaggg
      241 ctgttatgtt gcttgttagg ttttcactgg caatacacac gccactacta aataggagta
      301 atgttccgca aagaaccagc agtgtaacta atttttttcc attaattata ccgcctcctt
      361 ttaccagaaa aatatcgtgt cacaatgcta tgatttaact ggaatatgaa cttgtcatat
      421 tcccgatatc tctggcatac aagtctcttt tgactatctt ttatatagtt tatgattttt
      481 tttaaaagat aaaaccatct gtcctctatt ttcactatta aggcatcatt ttaaaggatt
      541 aatacaggtc caaaatgatt tagatttatg ggatgcccta attgaagaat gaaagccatt
      601 aaaaaacaat ttattcattt cagccctaat taggccctga atgattgatc acatggaccc
      661 tagtggagct tggagaagat gacagtggaa tctcaagggt taccaaggcc tataagcccc
      721 taaaacaggg aaaacaggaa tattaaaagc taaattatct atataaatag aatataattg
      781 attatataat ttaaaatcct aaattatctc ttaataatcc aactgtaaag cgtaggatgc
      841 ccagttctag ttactcatat tagatgtttt taaaaaggaa ttaaatggat taaaagcgga
      901 aacaggacta ttaactgttt ttaacaattt tgattctgga tgaaattgtg gtgcctgtgg
      961 gggttgtgga taggaaaaga atttaaaaga attgaactgg gttgttggta ctttgcccag
     1021 gatgtcaacc accttcatca aggagacgaa gagactccct gataaaaacc gggatatccc
     1081 cggggttacg gctggatacc aggttaccat ccaccaccac ctccgcgtct atgaactccg
     1141 ccccggcatt tttaatatcc tggattatgg atgtataacc agttaccttc ctgtcacgca
     1201 ggacatcggc ggtgatcaag agttgagggg cgtggcatat ggaaaaaacg ggcttaccac
     1261 tatctatgaa ttctttaacg aaccgaaccg ccacatcatg ggccctaagc cggtcagggg
     1321 agtaaccccc cggtattaaa agggcatcga attcatccac agaagccatc tccagggacc
     1381 tctcaatggt ggcctggacg gtgtggcgtt tgccactcac caccttccct gcctggagtc
     1441 caacaagaac caggtcatgc ccagcctcct taaaggccgc agctggttcg gtgcactcca
     1501 catcctcaaa ccagtcctcc agtataactg caattttagc caatcccatc accaccatat
     1561 atcttgatga tatttttgtg aagagcatta tttatttcct gggatacagg aactatccta
     1621 ccatggtggg gattggattt aaatcgttta gatctgttga aaataaaaaa attcaaagga
     1681 agaataagca ggtaaataag atctcatcac attactttat atgcttcaaa aatcaacagt
     1741 aatatgccta ttaggcaaaa attggaagtg aaagtttgtt tagagatagc tacagtaatg
     1801 atagagatag ttctgctccc attaaggaag ggcaggaata cgatgttaag attgaagata
     1861 caggcagaga cggtgacgga attgcccgta tcgaaggttt tgtggttttt gtttcaggcg
     1921 ccaaagtagg cgatgaagtt aagattagag ttaattctac ccgaagaaac tttgccttcg
     1981 ccgaagtagt agaataaatc cttattttct aaatagaagg ataatataaa ccatccctgg
     2041 tttattcctt atctattttt taacaaatgc aactctcacc taattcacaa agataatata
     2101 atatcattta tatatttttc aacttaattt ttattcccaa ctcaatttaa tcctatttca
     2161 caaaattaca gtcctattcc ttcttccaat aatttccagg gaaaagggtc accatgaccc
     2221 ggataaacat attttatgtc catttccttc aacctctcca cactggcccg ggccgcagcc
     2281 tcatcatcca tgatagcccc cagggcaggt tccttggtgt tggtgaacag atccccacag
     2341 aaaagatgcc cctcgctggt caggacgccg atggacccgg cagagtggcc gggtaggtgc
     2401 accacccggg cctccacccc gtagggtaga agatgataag catcctccac gtaaaagtcg
     2461 gggatgaatc ggtgcctttt ccctagaccc atgaaggggc ttatgatccg ggataacagg
     2521 tttcccctcc ggttgtacag catatccccc tgggtgacca tgcctgaatc agcctggtgc
     2581 atggcaactg gtgcttcgta cttatccctt aaaaatgcac agttaccggc atggtccgaa
     2641 tctccat
//
LOCUS       DAOQVW010000197         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_46549_length_2640_cov_4.109478, whole genome shotgun sequence.
ACCESSION   DAOQVW010000197 DAOQVW010000000
VERSION     DAOQVW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46549_length_2640_cov_4.109478"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKK85_05005"
     CDS             complement(<1..265)
                     /locus_tag="PKK85_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25411.1"
                     /translation="MEYFDILEQETEKLYQIATQARKKGFDVETEPEIPLAKDLAERV
                     EGLVGPKNVDLKIKELEDQMSREEIAFEIARIIVTSKDDEGKED"
     gene            618..779
                     /locus_tag="PKK85_05010"
     CDS             618..779
                     /locus_tag="PKK85_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HNS25412.1"
                     /translation="MAKKEKKCLPPSGAGLVRYFEEETKGPKMSPEQVVILTAILAIF
                     CIALRFSYS"
     gene            820..2169
                     /gene="purB"
                     /locus_tag="PKK85_05015"
     CDS             820..2169
                     /gene="purB"
                     /locus_tag="PKK85_05015"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNS25413.1"
                     /translation="MAIHPIEYRYGTPEMRSIWEAENKLQRMLDVEAALAEAEAELGL
                     IPGDAAMEIKNKASTKHVTWERVAEIERETNHDIAALVKGLAEVCDGEAGEYVHYGAT
                     SNDIIDTSQSLLLKDTLNILKDKVVRLARICLELAREHGETVCIGRTHGQHALPTTYG
                     MKFALWADELRRQYDRMQACQKRLCVGMMTGAVGTMAALGDDGLEVHRILSRILDLEP
                     VLISNQVVQRDNHAEYIMCLANLASTLDKMALEMRNLQRTEIKELGESFDPEKQVGSS
                     TMPHKMNPITAERICGVSRVVRAYVAPALQNNPLWHERDLTNSSAERIIIPESSILTD
                     YILQLSIKLMKNLVFYPENIEANLNLTGGLIMAERFMAELTRRGMGRQTAYGVVRRCA
                     LEANQKGISLEAVVLDNEDISAYLSPDEVCQIMDPHTYLGSAVQVVKNVLEDSKNWF"
     gene            2307..>2640
                     /locus_tag="PKK85_05020"
     CDS             2307..>2640
                     /locus_tag="PKK85_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HNS25414.1"
                     /translation="MINWGVVIVGFLAEIILGGILGVLIPGWGALLGLLLAGMLVGYM
                     VGGDAGNGAVNGAVAGAFGAIIISVLLLIFGTILLGLVGFAAASVTSMVLLLGSIGVI
                     IIMALGGAV"
BASE COUNT          736 a          554 c          622 g          728 t
ORIGIN      
        1 catcctcttt accctcatca tcctttgatg tgacaattat cctggctatt tcgaaggcaa
       61 tctcttcacg ggacatctgg tcttccagtt cctttatctt taggtcaaca ttcttaggac
      121 caacaagtcc ctccaccctc tcagccaggt ccttagccaa gggtatttca ggttcagttt
      181 ccacatcaaa acccttcttt cgggcttgag tggctatctg gtacagtttt tcggtttcct
      241 gttcgaggat atcaaagtac tccatgttac tcccattttt tctcttttta taaactcacg
      301 tggatggtaa gtcgggtttt tactagaaat cacctgtatt tggtgctgat acaagatctt
      361 ccttagtttt atatggaaca aggccacgta taatagattc ggtcctattt ttatagcccc
      421 taaaacctat ttttaataaa aaatatggtc ttaaacatct ttacaagtaa tctttcagtt
      481 tatctacata ttattaatct agttacccag agtatttttt ttagaagttc tgaattacca
      541 aactataaat aaagatgatg aaagatcatg ttctgttatt ggtgactaat taacataaga
      601 tcatctggtg attatacatg gctaagaaag agaaaaaatg cctacccccc agtggagcgg
      661 gactagtaag atactttgaa gaggaaacca aggggccgaa aatgtcccct gaacaagtgg
      721 tcattttaac cgccattctg gcaattttct gtattgcact aaggttttcc tactcttaat
      781 tttaaataca tctatttcta ttaaaaagga gctcttatta tggctattca ccctattgaa
      841 tatcgttatg gcactcctga gatgcgcagt atctgggaag cagaaaataa attacagcgt
      901 atgttagatg tggaggcagc tttggccgag gctgaagcag aactgggact catacccggg
      961 gatgctgcga tggagatcaa aaacaaagca agcaccaagc atgtgacctg ggaaagggtt
     1021 gctgaaattg agagggaaac caaccacgac atagccgccc tggttaaagg cctggctgag
     1081 gtatgtgatg gagaagcagg ggaatatgtc cattacggag ccaccagtaa cgatatcatt
     1141 gacaccagcc aatcactgct tttaaaggac accctgaata tcctgaagga caaagtagtc
     1201 cggctagcta ggatatgcct agaattagcc cgggaacatg gcgaaaccgt ctgcataggg
     1261 cggacacacg ggcagcacgc ccttcccact acttacggga tgaaattcgc cctgtgggca
     1321 gatgaattga gaagacagta tgatcgtatg caggcctgcc agaaaagact gtgcgtgggc
     1381 atgatgaccg gtgcagtggg caccatggca gccctgggag atgatggctt ggaagtgcac
     1441 cggatcctgt cacggatcct ggacttggag ccagttctga tatccaacca agtggtgcag
     1501 cgggacaacc atgctgaata catcatgtgc ctggccaact tggccagcac cctggacaaa
     1561 atggccctgg aaatgcgtaa cctgcagagg acagagatca aagagttagg ggaaagtttc
     1621 gaccccgaaa aacaggtagg aagtagtaca atgccccata agatgaatcc catcactgct
     1681 gagaggatat gtggagtttc tagagtggtg agggcttatg tggctcccgc tctccagaac
     1741 aatccattat ggcatgagcg ggaccttacc aattcttcag ctgaacgcat cattatccca
     1801 gaatcctcca tcttaaccga ttacatcctg caactctcca ttaaactaat gaaaaatctg
     1861 gttttctatc ctgaaaatat tgaagccaac ttgaacctca ctggagggct gattatggcc
     1921 gaaaggttca tggccgaatt aacccgcagg ggaatgggtc gccaaaccgc gtatggagtg
     1981 gtacgtagat gtgccctgga ggctaaccaa aagggaatca gtttagaggc agtagtactg
     2041 gataacgagg atatcagtgc gtacctatca cccgatgagg tttgccagat catggacccc
     2101 catacctacc ttggttcagc agtgcaagtg gtgaagaatg tgctggaaga ctctaaaaac
     2161 tggttttaga ggatagaatt aaatattttt catcctaatg atcttcaatt tcccccgagc
     2221 atgtggaaat ccctttattt tttttaaaaa aattaaaata gtgcaccgca gatactaata
     2281 cacgacaaat aatggggtgt gaagtattga taaactgggg tgtagtgatc gttggttttc
     2341 tggccgaaat tattcttgga ggaattcttg gtgttttaat acccggatgg ggagctctac
     2401 tgggattgct cctggcaggg atgctggttg gttacatggt aggaggagat gctggtaacg
     2461 gagctgttaa tggggcagta gccggtgcct ttggtgccat aattatttct gtactacttt
     2521 taatatttgg aaccatatta ctaggattag ttggttttgc agctgccagc gtcaccagca
     2581 tggtattact attaggttcc attggggtta tcatcatcat ggccctgggc ggtgctgtag
//
LOCUS       DAOQVW010000198         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_46748_length_2634_cov_2.454052, whole genome shotgun sequence.
ACCESSION   DAOQVW010000198 DAOQVW010000000
VERSION     DAOQVW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46748_length_2634_cov_2.454052"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKK85_05025"
     CDS             complement(<1..203)
                     /locus_tag="PKK85_05025"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HNS25415.1"
                     /translation="MMEKVYKEDEDWLKILTPEQFQVARKRGTEMAFTGKYHNCHEDG
                     IYQCVCCDTDLFDSKTKFESGTG"
     gene            complement(304..1230)
                     /gene="guaA"
                     /locus_tag="PKK85_05030"
     CDS             complement(304..1230)
                     /gene="guaA"
                     /locus_tag="PKK85_05030"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNS25416.1"
                     /translation="MLNSADFIKESIREIRDTIKDKKAIIAISGGVDSSVASVITAEA
                     IGDNLTAVFVDHGLLREGEAEYVENTFQDKLNFRLVDASAEFLRKLDGVEDPEEKRKI
                     IGEVFIRVFERVAEEVGAEFLVQGTIAPDWIESEGNIKSHHNVALPHGLVLEVVEPIR
                     ELYKDEVRILGSELGLPEHITQRQPYPGPGLAVRIAGVITPEKISICRQANAIVEEEV
                     HKEGLDKTLWQYFAVLTNTKVTGVKGDLRDYGYLVVVRMVESQDAMTADVPKLPWIMI
                     KTISQRITAEIPEVTHVALSVSDKPPSTIELA"
     gene            1312..>2634
                     /locus_tag="PKK85_05035"
     CDS             1312..>2634
                     /locus_tag="PKK85_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25417.1"
                     /translation="MKENLKISKNIYSNPNLFFSLVLIIYVFLGVYFLRYYKYDLISK
                     DIISFVSVANLYASGNFSSAINGYWGPLFSWVLVPFILLNSSPYFVLNMMKIISLVAG
                     IFTIYGVRLLSYRFDMSEQIRNSILVAAIFMVLYFALRSSPVDLIFLCFLLYYLYYIF
                     SPKYSLKPYYGVICGLIGALAYFTKSYAFTFFPIHFVLFNIIHYLNGGNYRKTVLKNL
                     ILGLTVFFIISGTWSFILSEKYGEITFGTSAKYNHAVIGPYSTGSHPTEYINQGFMKP
                     PYPGVTSAWYDPTFFTVKPWSPIDSWYNFSFQLRKIRDNIRGLIGLYSSFSHLSFAII
                     FSYVLFLVRPFKELISRRDLLFPLLTLLIFPLGYLPIALEIRYLWVVYVLLILMGGYL
                     LNLLLKKGFFNRAQMSILMVIFIFSFILLPVSGLNSNKYTGEEYAELGR"
BASE COUNT          711 a          542 c          426 g          955 t
ORIGIN      
        1 cacccagtac cagattcaaa ctttgtttta gagtcaaata agtcagtatc acagcaaaca
       61 cattgataaa taccatcttc atggcagtta tgatattttc cagtgaaagc catctcagta
      121 cctcttttac gggctacttg aaactgttct ggagtcaata tcttaagcca atcttcatct
      181 tccttataaa ccttctccat catcttaatt tttccagatt tcagcatgaa gatggggatt
      241 tcattggaac tatggttcat gattataata ctatggctgc cactacttta acttattttc
      301 atcttaagcc aattcaatgg tactaggggg tttatcactc actgaaagag caacatgtgt
      361 tacttcaggt atttctgctg tgatgcgttg agaaatggtt tttatcataa tccagggaag
      421 ttttggaaca tcagctgtca tggcatcctg ggactccacc attcgcacaa ccaccaggta
      481 cccataatcc cgaagatctc ctttaacacc agtaactttg gtgttggtca aaaccgcaaa
      541 gtactgccat aaagttttat ccagaccctc tttatgaact tcttcttcca caatggcatt
      601 ggcttggcga cagatactga ttttctctgg agttataacc cctgcaattc gcactgccaa
      661 tcccgggcca gggtatggtt gtctttgggt gatatgttct gggaggccta attcagagcc
      721 taaaatcctt acttcatctt tatatagttc ccttattggc tccacaactt ccagaaccaa
      781 tccgtggggt agggccacat tgtggtggga tttaatattt ccctcactct cgatccagtc
      841 cggggcgatg gttccctgca ccaagaattc ggctcccacc tcttcagcca ccctttcaaa
      901 aacccggatg aagacttccc ctatgatctt ccttttctct tcaggatcct ccactccatc
      961 cagttttcgc aggaattcag cacttgcatc caccaacctg aagttaagtt tatcctggaa
     1021 agtgttctca acgtattctg cttcaccctc ccgtaaaagt ccgtggtcaa caaaaactgc
     1081 tgttaaattg tctccaatag cttcagctgt aattactgat gcaacagaac tgtcaacccc
     1141 acccgaaatg gctataattg cttttttatc tttaatagta tctcttattt ctcgtatgga
     1201 ttcttttata aaatcagccg aattaagcat tttttcacct actctaataa ataatttaac
     1261 tagggcaacc tatcacaata gtaatttatt taacttaact aattattttt gatgaaggaa
     1321 aatttaaaaa tctcaaaaaa catatattct aacccaaatc tcttctttag ccttgtattg
     1381 ataatttatg tctttttagg ggtttatttc cttagatact acaaatatga tttaattagt
     1441 aaagatatta tcagttttgt ttcagtagct aatttgtatg cttctggaaa cttttccagt
     1501 gctataaatg gttactgggg gccattattt tcatgggtac tggttccatt tattcttcta
     1561 aattcatccc cctatttcgt tctgaatatg atgaaaatta tatctttggt tgccggtatt
     1621 tttaccatct acggtgttag actcctatct taccgatttg atatgagtga acagattaga
     1681 aattcaattc tggtggcagc aattttcatg gtactatatt ttgcgctccg ttctagccct
     1741 gttgatctaa tatttctttg ctttttactt tattatttat attatatttt cagcccaaaa
     1801 tattctttaa agccgtatta tggcgttatt tgtgggttaa taggtgcctt agcttatttt
     1861 actaaaagtt atgcctttac tttctttcca attcacttcg tattattcaa catcattcat
     1921 tacctcaatg ggggaaatta tagaaaaacc gttttaaaaa atctaatttt aggtttaacc
     1981 gtttttttta taatcagtgg cacctggagt ttcatactca gtgaaaagta tggtgaaatt
     2041 acctttggta catctgccaa atataatcat gcagtaattg gaccttattc tactggttca
     2101 catcccacag agtacatcaa tcagggattc atgaaacctc cttatcccgg tgtaaccagc
     2161 gcatggtatg atcctacttt cttcactgta aaaccttgga gccctataga ctcttggtat
     2221 aattttagtt ttcagttaag aaaaattagg gataacattc gaggtttaat tggtttatac
     2281 agtagttttt cccatctttc ctttgccatt attttttctt atgttttgtt tctggtacgg
     2341 ccttttaaag aattaatttc tagaagggat ctcttatttc ctttgttaac ccttttaata
     2401 ttccctttag gatatttacc catcgctcta gaaattaggt atctctgggt tgtctatgtg
     2461 cttctaatat taatgggggg ctatttactc aatttacttt tgaaaaaagg tttctttaat
     2521 agggcccaaa tgtcaatttt aatggtaatt tttatttttt cttttatcct gttacctgtt
     2581 tccggcctaa actcaaataa gtacactggg gaggaatatg ctgaattagg ccga
//
LOCUS       DAOQVW010000199         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_47048_length_2623_cov_4.030374, whole genome shotgun sequence.
ACCESSION   DAOQVW010000199 DAOQVW010000000
VERSION     DAOQVW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47048_length_2623_cov_4.030374"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..1072
                     /locus_tag="PKK85_05040"
     CDS             449..1072
                     /locus_tag="PKK85_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNS25418.1"
                     /translation="MHLPDGIIPLPQALIYWIITLIFVAIYLFKLSKTEHREKNVVYT
                     SIFAAATVATSSLSIPSPLGVPIHFFLIPLVAILMGPLSGVIVAFLCLVIQFFLMGMG
                     GITTLGANTLAIGVAISVVTYFFYSIIRDLNWRLSIFSGTFLGIMVATIVQVLMLFFA
                     GLATIDVLLASLIPFYLLIAVIEGFANVIIVSFLSKVKPELVNLDKI"
     gene            1087..1578
                     /locus_tag="PKK85_05045"
     CDS             1087..1578
                     /locus_tag="PKK85_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25419.1"
                     /translation="MMNIKSTILFLVVIFGLMIPVSAHGVHVTPDSATAIVIADNSTG
                     SIAKRTVDEMGMNVTVYSFQSDVDVNHELEHLLTNPNKKVLAVGYLDTVNKFLAQHPE
                     VSDRIIVCNPDPETIRKGLNRLDSTPTESSYGFLTPFLTGALIGALSGIGVGAFLMKR
                     KLS"
     gene            complement(2002..>2623)
                     /locus_tag="PKK85_05050"
     CDS             complement(2002..>2623)
                     /locus_tag="PKK85_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNS25420.1"
                     /translation="GLIFGILIILLTVFLIYLPKMKSGGRSTAASREIPFALRQMATE
                     LRAGMGLHDSMRSVATSGYGPLSEEFARALEEIKYGQTTEQALMDMSERIDSEGLKRA
                     VYQITRTLTSGGDLAKTLGVIAEDTAYEMRMKLKDYAQKLNSFTMIYMFIAILGPVII
                     MVMLIAASTVMGPMIPPILLLIAYLFFFPAIVAFMAFMIKRLEPKL"
BASE COUNT          757 a          540 c          516 g          810 t
ORIGIN      
        1 cgcttgatca gaagtagttt cttcacctag ttcttcaccc ccagtgttag atgaatctgc
       61 agctgataca gctccacaga gtaaaacagc catgactgct acaagaacta gtactgttcc
      121 ttgctttttc atcctttttc acctcctatt tattcttagt gcgactttta aaaagtcgag
      181 ccatagtgtg tagtaatact atatataatg tttatgttat tactatgaag tgttatctat
      241 ggagaaaaag taatactaac taaaaaatta atactaaaat tcagatatat ccccctatag
      301 taatactgac gggggattaa atagtaaatg ttatattcgt aaacaatgat ttatagtaag
      361 atcgatgaat tcatttctcg accgggtaat aacagggctc ctggtcgaag atgagaatta
      421 aaaattcaat ataaaagagg taattagttt gcatttacca gacggaataa taccattacc
      481 ccaagctttg atttactgga tcatcaccct catcttcgta gccatatact tgttcaaact
      541 ttccaagaca gaacaccggg aaaaaaatgt ggtgtacacc tccatctttg ccgctgctac
      601 ggtagctact tcttctctat ccataccctc acccctggga gttcccattc atttcttcct
      661 aatacccctg gtagccattc taatgggccc cttaagtggc gtgatcgtgg cttttttatg
      721 cctggtcatc caatttttct taatgggaat gggtggaata accactctag gggctaatac
      781 cctggccatt ggtgtagcca tcagtgtagt cacctacttc ttttacagta taattcggga
      841 tctgaattgg aggctaagca ttttctcggg aaccttcctg ggaataatgg tagccaccat
      901 agtacaggtc ctgatgcttt tttttgctgg ccttgccact atagatgtac ttctggccag
      961 tttaatacca ttttatcttt taattgctgt gatagaggga tttgccaatg ttattatcgt
     1021 atcttttcta tcaaaagtca aaccagagct agtaaacctg gataaaattt agaggaggac
     1081 ttaaacatga tgaatataaa atccaccatc ctgttcttgg tagtaatatt tggattgatg
     1141 atcccagtga gtgctcacgg agtacatgtt actcctgatt ctgccacagc aattgtcatc
     1201 gccgataatt caactggatc aattgcaaaa agaacagttg atgagatggg tatgaatgta
     1261 accgtgtata gtttccaatc tgacgtggat gtcaatcacg aactggaaca tctgctcacc
     1321 aatcccaata agaaagtgtt agccgtagga tacttggaca cagttaacaa gtttctagcc
     1381 caacatccag aagtttccga tcggatcatt gtatgcaatc ctgatcctga aacaattagg
     1441 aaaggtttaa accgcctaga ttcaaccccc acagaatcca gttatggttt tttaactcca
     1501 ttcctgaccg gcgctctgat tggtgcttta agcggaatag gtgttggagc attccttatg
     1561 aagaggaaac tatcctaaac tattttttta ttcttaattt ttaaataaat atcctggatg
     1621 tgaggattaa agggtgatcc tagttccagt aaaaatatta taagttaata taggcagggc
     1681 atcctatcta gaggcacgtt tgaggttttt ataatattta gatgccctgc actgatttct
     1741 gtagggacta tggcacttat ccctccggga attcaggtca tgatgtctga caatagaact
     1801 tgtaaccacc attataaata cttttttctt atttaatatt actttttact taaaaatatt
     1861 ggaattttta ttaccaacat cttctgtaat tggactgtag ttttccagag ctaatggttt
     1921 aaccattgtc tctattgtcc cattgctatg gcgtaaaaaa aattctgtgt gaaaaaatag
     1981 aaaggaaaag aatgatttga tttaaagctt gggctccagt ctcttgatca tgaaggccat
     2041 gaaggccacg atggctggga agaagaagag gtaagcaatg agtagcagga taggtggaat
     2101 cattggaccc attactgtgg aggccgcaat taacatgacc atgatgataa ccggtcccag
     2161 aatggctatg aacatgtaaa tcatggtgaa ggagttcaac ttctgggcat aatccttgag
     2221 cttcatcctc atctcatagg cggtgtcctc ggcaataact cccagggttt tggccagatc
     2281 ccccccactg gttagagtcc tggtgatctg atacaccgct cttttgagac cttcagaatc
     2341 tattctctca ctcatatcca ttaatgcttg ctcggttgtt tgcccatact tgatctcctc
     2401 cagtgcccgg gcaaattcct ctgaaagggg gccgtatcca gaagtagcca ccgatcgcat
     2461 actgtcgtgt aaacccattc cagcacgaag ttcagttgcc atctgtctca gggcaaaggg
     2521 gatctccctg gaagcagcag tagatcttcc ccctgatttc attttaggca gatatatgag
     2581 gaacacagtc aatagaataa ttagtatacc gaagataagt ccg
//
LOCUS       DAOQVW010000200         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_47077_length_2622_cov_4.008181, whole genome shotgun sequence.
ACCESSION   DAOQVW010000200 DAOQVW010000000
VERSION     DAOQVW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47077_length_2622_cov_4.008181"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..182
                     /locus_tag="PKK85_05055"
     CDS             21..182
                     /locus_tag="PKK85_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNS25421.1"
                     /translation="MDKIECTSCKQEISPVETYVKFECPECQEILFRCQKCRTFGHMY
                     QCKCGFKGP"
     gene            250..519
                     /locus_tag="PKK85_05060"
     CDS             250..519
                     /locus_tag="PKK85_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HNS25422.1"
                     /translation="MGDVVATIKLMPESPDVDLEKIKADVEKSIPSGTELHKIEEEPI
                     AFGLVALKVMVVVGDAEGGTEPVEENLAQVDGVNSVEVVDVRRLM"
     gene            691..1935
                     /locus_tag="PKK85_05065"
     CDS             691..1935
                     /locus_tag="PKK85_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNS25423.1"
                     /translation="MVDLILACFIGIFCGAVTGLIPGIHVNTVGAFVFASSTFLLNFH
                     SPETISVFLISMSISHALLEFIPSMFLGVPEEGTVLSILPGHQMLLQGRGKEAIRLVA
                     LGGFGSVLVIIALLPMFMMVLPPLYGLLKPYIWIMLIIVVVVMIIRLSRDRLSMFWSS
                     LLFVLSGIMGWTLLNSSLPTSVSFLSMFTGLFGVSTMLYSLSQNSFLPEQNQHHKVTI
                     NTPVIRGIFAGGIAGSILGFLPGLGPAQGSMIAQELSGGSDAENQREGFLVAMSGVNV
                     SDALFSLMAIYLIGNPRSGIAVYVNHLLNGVNFAQLLVFIFTSVTAVSVALILCLKLG
                     DAAGENLEKFDYQKLTWSVIIFMSFLVGLFSFIEQANILMVVLTYITSISLGLLPHYL
                     GINKSHLMGVLIVPAIVVYMGL"
     gene            complement(1940..>2622)
                     /locus_tag="PKK85_05070"
     CDS             complement(1940..>2622)
                     /locus_tag="PKK85_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNS25424.1"
                     /translation="PLKSPPYLGKDSLVLLTDEIDHKSALRIYKEVPEVRGVLKGDLK
                     ETVGVTDSESSPHTYQLLKGCDLRCDVLETPSGPICIYKNQGQIHIEFPKPSNPKITV
                     LHETMDQYSNPSVLDCTCGPGTLGISALKSGARKVVFNDIWYPAAWITSVNLGVNGFP
                     VEFSQKKQGLIGEGDNFQVYCADLRDLKPFLDEKYDIGVIDPFPGVDFTEFSQIMGEI
                     CHEVIIIG"
BASE COUNT          712 a          540 c          564 g          806 t
ORIGIN      
        1 cattcattgg aggagattaa atggataaaa tagaatgtac atcttgtaaa caagagatat
       61 cccctgtaga gacttatgta aagtttgaat gtccagaatg tcaggagatt ctctttcgct
      121 gtcagaaatg cagaaccttt ggccacatgt accagtgcaa atgtggattt aaagggccgt
      181 gatggcctta tttaatttga tatcttgaag atttaaaaac cataaaaaac aattagggga
      241 ggaaatacta tgggagacgt ggttgcaact ataaaactca tgcctgaaag ccctgatgtg
      301 gacttggaaa agattaaagc tgatgtggaa aaatccatac catcagggac ggaattacat
      361 aaaattgaag aggaaccaat cgcatttggc ctggtggccc ttaaagtgat ggttgtggtt
      421 ggggatgccg agggtggaac cgaaccagtg gaagaaaatc tggctcaggt tgatggtgtt
      481 aacagcgtag aagttgtaga cgtgcgccgg ctcatgtaac tgtgggatca gccgtttttt
      541 tttattttta taattttttt tatatttcca gaaatcattg attatactta tactctggat
      601 ccatcacctt gaataataat tttttaccaa aaaatattaa ttatcggttc gtcataagat
      661 catgcaaaaa agatctggct ggtttcctac atggttgatc tgatcctggc ttgttttatt
      721 ggcatattct gcggtgcagt aactggtcta attcccggga tccatgttaa cacagtaggg
      781 gcctttgttt tcgcatcttc tacattcctt ttgaattttc actcccccga aacaatctct
      841 gtcttcctaa tatccatgtc cattagtcac gccctattag agttcattcc atccatgttt
      901 ctgggtgttc cagaggaggg aacagtacta tccatactcc ccggacacca aatgttgctt
      961 cagggaagag gaaaagaagc catccgctta gtggctttgg gaggatttgg ctcggttctg
     1021 gtgattatcg ctttgctacc aatgtttatg atggttctac ctccacttta cgggctgcta
     1081 aagccatata tatggataat gctcattata gtggtggtgg tgatgataat tcgtctcagc
     1141 agggataggc tgtccatgtt ctggtcatca ctcctattcg tactgtctgg gattatgggc
     1201 tggaccctcc taaattcatc cctgcccacc agtgtatcct ttctgagcat gttcactggt
     1261 ctttttgggg taagcaccat gctttacagc ctatctcaga actcgtttct acccgaacag
     1321 aatcaacatc acaaagtgac cataaacacc cctgtgatta ggggaatttt tgcaggagga
     1381 attgcaggga gcatactcgg gtttttacca ggattgggtc cagctcaggg tagcatgatc
     1441 gcccaggaac tcagtggagg tagtgatgcc gagaaccaga gggaaggatt tctggtggcc
     1501 atgagcggag ttaatgtttc tgatgcttta ttttctctaa tggccattta tttgatagga
     1561 aatcccagga gtggaatagc agtttacgtt aaccacctct taaatggggt taattttgcc
     1621 cagctcctgg tttttatatt cacatccgta actgcagtat cagtagccct gatcttgtgt
     1681 ctcaaactgg gagatgctgc cggtgagaac ctggaaaaat ttgattatca aaaattaact
     1741 tggtctgtaa tcatattcat gagcttccta gtaggactct tctcatttat agaacaagca
     1801 aacattctaa tggtggtgct gacctacata acatccatat ccctgggttt actcccacat
     1861 tatcttggta ttaacaaatc acaccttatg ggggtactaa tagtgccggc tatcgtggtt
     1921 tatatggggt tgtgaggatt cacccgatta taatcacttc atggcagatc tcacccatga
     1981 tttggctaaa ttccgtaaaa tctacgcctg gaaagggatc tatcactcca atatcatact
     2041 tttcgtctag aaacggtttc agatcccgga gatcagcaca gtaaacctga aagttatcac
     2101 cttctcctat taatccctgt tttttttggc taaattccac aggaaaaccg tttactccaa
     2161 gattaacact ggtaatccag gcagccggat accagatgtc attaaaaact actttcctag
     2221 ctcctgactt aagggcagat attcccaagg ttcccggccc gcaagtacag tccagaacac
     2281 ttggattcga atactgatcc attgtttcat gaagaacagt tatcttggga ttagatggct
     2341 tcgggaactc tatatggatc tggccctggt ttttgtagat acaaatcgga cctgatgggg
     2401 tttctaaaac atcacacctc agatcgcatc ctttcagcaa ctggtaggtg tgtggagatg
     2461 actctgaatc tgtgacccct actgtttctt ttagatcacc ttttaaaaca cccctaacct
     2521 caggtacctc tttatatatt ctcaaggcag atttatgatc aatttcatca gttaacagga
     2581 ctaacgaatc ttttcccagg tatggaggac tttttaatgg at
//
LOCUS       DAOQVW010000201         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_47789_length_2598_cov_2.937082, whole genome shotgun sequence.
ACCESSION   DAOQVW010000201 DAOQVW010000000
VERSION     DAOQVW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47789_length_2598_cov_2.937082"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PKK85_05075"
     CDS             complement(<1..1140)
                     /locus_tag="PKK85_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNS25425.1"
                     /translation="MKSIRDSVHGDLQLDEFELKLVDTPEIQRLRRIKQLGFTYLVYP
                     GANHTRFEHSMGTMYLAGRMALNLDLDPDKKTLIRVCAALHDAGHGPFSHVSEGVLET
                     SHEKLTTHLIKKSALGDILSERYNLKEVVDIISGQGPLGQIVSGDLDVDRMDYLLRDS
                     YYTGVAYGIIDVERLINNMHLDGENLTLHRKGVQAAEAMLLARYFMYPSVYQHHTTRI
                     VNAMFRRCLSHLFKGGVIQSPEIYRYDDADIVSAARNEKGYIRDIIHRLDNRQLFKRV
                     YALKLNNLEDPMTVFSMDDARNRQSEVEIAEDLGVEEDYILIDPPEYPTFNESAPVSV
                     YGSTKTLGEVSNLVVALEHARFNHADLSIYMPQEAASRVKGFPFRD"
     gene            complement(1142..1579)
                     /locus_tag="PKK85_05080"
     CDS             complement(1142..1579)
                     /locus_tag="PKK85_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNS25426.1"
                     /translation="MTIKLIRPDEDRITIYQLIDEMKNIPQINECGAIFTFEGLVRGE
                     EKELSTHALNLTTTDYDKTQEELENLVEDVKDKHQVKEVAVVHYLGQFQPGDPLFLVA
                     VAGAHRQETRSALNEVIERVKYELDFQKEEETNQGNRIIMSGG"
     gene            complement(1576..2112)
                     /locus_tag="PKK85_05085"
     CDS             complement(1576..2112)
                     /locus_tag="PKK85_05085"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012956066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HNS25427.1"
                     /translation="MVRIKRGLLVGRMQPVHLGHMQVIKTILQEVDELIIGIGSAQLS
                     HSIKDPFTAGERVMMLTKSLSENNIVASRYYVLPIQDVACNSVWVSHLEMLTPPFSKV
                     YSGNPLVQRLFVEAGYEVKIPPLFNRKNYSGTEVRRRMLADEDWQSLLPRTVVEVIHE
                     ISGVARIKHLNRKEVSEV"
     gene            2403..>2598
                     /locus_tag="PKK85_05090"
     CDS             2403..>2598
                     /locus_tag="PKK85_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS25428.1"
                     /translation="MITVKNLSKTFQTEDGVQIPALTDVSFQVVPGEIVGIIGTSGSG
                     KSTLLRIIRGVESFDEGEITI"
BASE COUNT          608 a          654 c          660 g          676 t
ORIGIN      
        1 atcccggaag ggaaatccct tcacccggga tgccgcctcc tggggcatgt aaatggacaa
       61 gtctgcatgg ttgaaacggg catgctccag ggcaaccacc aggttagaaa cctctcccag
      121 agtcttagtg gagccataca ccgatactgg tgcgctctcg ttgaaggtgg gatactcggg
      181 ggggtcgatg aggatgtagt cctcctccac tcccaggtcc tctgctattt ccacttcgga
      241 ctggcggttc ctggcatcgt ccatactaaa aacagtcatg gggtcctcca agttattcag
      301 cttaagggcg tagacccgtt taaagagttg ccgattatcc aggcgatgga tgatgtcccg
      361 gatgtagccc ttctcattcc gggcggcgct gactatatcc gcatcatcgt agcggtagat
      421 ctcaggggac tggataacac cccctttaaa tagatggcta aggcagcgcc tgaacatggc
      481 gttgacgata cgggtggtgt ggtgctggta cacgctgggg tacatgaagt accgggccag
      541 gagcatggcc tccgcagcct gcacaccctt acggtggagg gtgaggttct ctccatccag
      601 gtgcatgttg ttgatgagcc gctccacatc gatgatcccg taggccaccc cggtgtagta
      661 ggagtcccgg aggaggtagt ccatacgatc cacgtccaga tccccggata cgatctgtcc
      721 caggggtccc tgtccgctga tgatgtccac cacttccttc aaattgtacc tctcggataa
      781 gatgtctccc agggctgatt ttttaattaa atgtgtggtg agcttttcat gggatgtttc
      841 caacactccc tccgagacat gggagaaggg tccgtgccca gcgtcgtgca gggcggcgca
      901 gacccggatc agggtcttct tatcagggtc caggtccaga ttcagggcca tcctaccagc
      961 caagtacatg gtgcccatgg agtgctcgaa acgggtgtgg ttagcaccgg gatagaccag
     1021 gtaagtgaat cccaactgct ttatacggcg cagtctctgg atctccgggg tgtcaaccag
     1081 ttttaattca aactcatcca attgcaggtc gccatggacg ctgtccctga tggacttcat
     1141 attaccctcc ggacattatg atacggttac cctgattggt ttcctcttcc ttctggaaat
     1201 ccagttcgta cttaaccctc tcgataactt cgttaagggc ggatcgggtt tcttgccggt
     1261 gggccccggc taccgccacc aaaaacaggg ggtctccggg ttggaactgg cccaggtagt
     1321 gaaccaccgc cacctccttg acctggtgtt tgtccttaac atcctccacc aagttttcca
     1381 gttcctcctg ggttttatca taatcagtgg tggtcaagtt cagggcgtgg gtgctcagtt
     1441 ccttctcttc gccacgcacc aggccttcga aggtgaatat agctccacat tcattgatct
     1501 gggggatgtt cttcatttca tctattaact ggtagatggt gatccggtct tcatctggtc
     1561 ttatgagttt aatggtcaaa cttcgctcac ctccttgcgg ttgagatgtt taattcgggc
     1621 tactccacta atttcatgga taacctccac tacggttctg ggtaagaggg actgccagtc
     1681 ctcatcagcc agcatcctcc gccttacctc ggtccccgag tagtttttcc tgttgaaaag
     1741 tggtggaatt ttaacttcat atccagcctc tacaaagagt ctctgaacca gtggattgcc
     1801 agagtagacc ttgctaaaag gcggtgtcag catctccagg tgagacaccc agacactgtt
     1861 acaggcaacg tcctggatgg gcagcacgta gtaccgtgat gcgacaatgt tattttctga
     1921 tagggacttg gtaagcatca taaccctttc tccagcggtg aaggggtcct tgatactgtg
     1981 acttagctgg gcgcttccaa tgccaatgat aagctcatcc acctcctgca gtatggtttt
     2041 aatcacctgc atgtgaccca ggtgcacggg ttgcatgcgg cccaccagta agcccctctt
     2101 gattctcacc atgtatccct caagctatct catccataaa aatattttag attttatata
     2161 tttataatgg gtggttacag aaccccaacc ctgggtgctg gtaatcctag tggaaaggga
     2221 actgtccctg ccacacatga gatggggatc caggactctg tggaatttaa aagctggtaa
     2281 agaaaaatct tttaaatcaa tatcaacaac taggttccag gactaaatat ttttataaga
     2341 aaagaagatg gccaaagcaa ccattgattt aaatctaaat gatattcagg gtgattaaaa
     2401 aaatgataac tgttaagaac ttatcaaaaa ccttccaaac tgaggatgga gtgcagatcc
     2461 cggcccttac tgatgttagc ttccaggtgg taccgggtga aattgtgggg ataattggaa
     2521 ccagtggctc tggtaagagc accttactgc gcataatcag gggtgtggaa agcttcgatg
     2581 agggggagat aaccatag
//
LOCUS       DAOQVW010000202         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_48068_length_2588_cov_3.588630, whole genome shotgun sequence.
ACCESSION   DAOQVW010000202 DAOQVW010000000
VERSION     DAOQVW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48068_length_2588_cov_3.588630"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PKK85_05095"
     CDS             complement(<1..166)
                     /locus_tag="PKK85_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HNS25429.1"
                     /translation="MVRRKRRILSDHDEDPMAGSANLVDAMLVLSVGFLIFLVLSWNM
                     QNVIFADMTPE"
     gene            complement(129..806)
                     /locus_tag="PKK85_05100"
     CDS             complement(129..806)
                     /locus_tag="PKK85_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNS25430.1"
                     /translation="MVAIPGSEILGSALHVISQSLLIPVIIGLLAFMIYAIVSFGGLI
                     SEYTSRIRIETKQIEKIISEFSNPGTPDNIIEIMEQNQDLPYGYRETITRIASHPEIG
                     SKSREALARKLIEKEEARAAKSLEKTDMVTRLGPTLGLMGTLIPMGPGLAALGAGDIN
                     ALAQAIIIAFDTTVVGLAAGGIAYVISKVRRRWYEEYLSNLDALCESALEVMEHGQTQ
                     TTYPVRS"
     gene            complement(819..1565)
                     /locus_tag="PKK85_05105"
     CDS             complement(819..1565)
                     /locus_tag="PKK85_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2162 domain-containing protein"
                     /protein_id="HNS25431.1"
                     /translation="MMELLWKLGILSVVLVFGVKIGLAMGFAGLSRKVAAAIILGYGG
                     GILLLTYIASGFVDQLQGFVYDYSSILGIAMAAIILYAGFHTLKEWKINEKNSISATC
                     LAMIAPCPCCFGAAVAAIIIAAPMIGASAMVVGEYAVLTLIVVMTVCYLISGLIGRAL
                     NKPYPVLLGNFMLFAGFYFLTSAIVIPNFGTVLNQEMSPLNIPDLWMFIFALLIVVIL
                     AVAGYYMTSRKSPLLNQKSDSTSEILKKNT"
     gene            complement(1737..>2588)
                     /locus_tag="PKK85_05110"
     CDS             complement(1737..>2588)
                     /locus_tag="PKK85_05110"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS25432.1"
                     /translation="GNPDAILADIRNKNNVITRTIKEEVELEIRSQLLNPTYQDSLLG
                     TASGWIEYASRYENLFGMQATTGCVSNKMWTMAAQNLLNDRFTPVTDYQASATQSMIG
                     WVIEAARRDMWQPDAQLLSTLKDKYIQSVNEYGVCCCHHTCGNLAFNQYILVGSSLGT
                     AQLQQYSAVVQDATGELITNGTSGSTETSAQNGQTGPNSKNTRGDETSRGRVLLGDVS
                     ATQVTSTSEPQVSGGDGNGYEVSMVSPQGSPQSNMPLVALVGVLLLVGLVGLGYFRSN
                     IQGWFRK"
BASE COUNT          693 a          607 c          547 g          741 t
ORIGIN      
        1 cctcaggggt catgtcggca aagatcacat tttgcatgtt ccaggagagt accaggaaga
       61 tgaggaatcc taccgagagt accagcatgg catctaccag gttggcagaa ccagccatgg
      121 gatcttcatc atgatcggac aggatacgtc gtttgcgtct gaccatgttc catcacctcc
      181 agtgccgatt cacacaatgc atccaagttt gacagatatt cctcatacca tcttcttcgg
      241 accttggata ttacataggc tattccacct gctgctagac ccaccaccgt ggtatcgaag
      301 gcaatgatta tggcctgggc caaagcatta atatcccccg cacccagggc agccagacct
      361 ggccccatag gtatcaaagt tcccatcaac cccagggtag gacccagacg ggtgaccatg
      421 tccgttttct ctaagctttt agcggctctg gcctcttcct tttctataag ttttcgggcc
      481 agggcttccc gagatttgct accaatctca gggtgagaag ctatcctggt gatggtctcc
      541 cggtacccgt agggaaggtc ctggttctgt tccatgatct ctataatatt atccggcgta
      601 cccgggtttg agaattcact aattattttt tctatctgct tggtttcaat tcttattcta
      661 ctggtgtatt cggagatcag tcctccgaag cttactatgg cgtagatcat aaaggccaaa
      721 aggccaataa ttaccggtat tagtaggctt tgggatatga cgtgtaatgc tgaacctaat
      781 atttcactgc ctggaatagc taccataatt taatcaccct atgtattttt tttgagaatt
      841 tctgatgtgc tgtctgattt ctggtttaaa agaggacttt tacggctagt catataatag
      901 ccagccactg ccaatattac tacgatcagc agggcgaata taaacatcca gagatcagga
      961 atgtttaaag gactcatttc ttgatttaaa actgtgccaa aattgggaat tacaatagct
     1021 gaagttaaaa agtagaaacc agcaaataac atgaagttac ctagcaaaac tggatagggt
     1081 ttgtttaatg ctcgccctat tagtccagag atgaggtaac ataccgtcat gactacgatg
     1141 agagttaaaa cggcatattc tcccaccacc atggccgagg caccgatcat gggtgcagca
     1201 atgatgatgg ctgccactgc ggctccaaaa caacaggggc agggagcgat catagccaag
     1261 caagtggcgc tgatactgtt tttttcgttt attttccatt ccttcagggt atgaaatcca
     1321 gcgtagagga tgatagcggc catggcaatt cccaagatcg aactataatc atatacaaat
     1381 ccctgaagct gatctacaaa accactggca atgtaggtta gtaagagtat gcctccccca
     1441 tatcctagga ttatagctgc agctactttc ctggataagc cagcaaatcc catggccaga
     1501 ccaattttaa ccccaaaaac cagtaccact gataatattc ccagcttcca tagtaattcc
     1561 atcatgttaa tcaccataag gtttggccaa atactttata atactttcta tttaatttat
     1621 attattactt atttagctaa tcgagatttt ttgtaatact tttgaatatt ttaatacaat
     1681 attccagttt ttttatagta ttattaatat tatataaaat aaagaagaat acagggttat
     1741 ttcctgaacc acccctgtat attgctccgg aagtagccta agcccaccag tcccaccagt
     1801 aacagtaccc caaccaaggc caccaagggc atgttggact ggggtgagcc ctgggggctg
     1861 accatgctga cctcataacc attaccatcc cctccactta cctgtggctc ggatgtggac
     1921 gtgacttggg ttgcagatac atcaccaagc aaaactcttc ctcgggacgt ttcatcaccc
     1981 ctagtgtttt tagaatttgg tccggtctgc ccattctgag ccgaggtttc agtacttcca
     2041 gaagtgccat ttgtgattaa ttcgccggta gcatcctgca caactgcaga gtattgctgt
     2101 aattgagctg tgcctaggga agaacctact aagatgtact ggttgaaggc caggtttcca
     2161 caggtgtggt ggcagcaaca caccccatac tcattgacgc tttgtatata tttgtccttt
     2221 aatgtggaca ggagttgggc atcgggttgc cacatatctc gacgggcagc ttcaataacc
     2281 caccctatca tgctctgggt agcagaggct tggtagtcag tgactggagt gaaacggtca
     2341 tttaagagat tttgagcagc cattgtccac atcttgtttg aaacacaccc tgtggtagct
     2401 tgcatcccaa acaaattctc gtaacgggag gcatactcta tccaaccact ggcagtgccc
     2461 agcagcgaat cctggtaggt tgggttaaga agctgggatc ttatctccag ttcaacttct
     2521 tcttttattg ttctggttat cacattattt ttgtttctaa tatctgccag tatggcatca
     2581 ggatttcc
//
LOCUS       DAOQVW010000203         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_48177_length_2584_cov_3.182285, whole genome shotgun sequence.
ACCESSION   DAOQVW010000203 DAOQVW010000000
VERSION     DAOQVW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48177_length_2584_cov_3.182285"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKK85_05115"
     CDS             <1..411
                     /locus_tag="PKK85_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNS25433.1"
                     /translation="LLHDSFWYNNTAQNAKLAQEIVEYNTGLHSDAVVVFTPQAVDAM
                     IASVGGVYIEGVGTVKGNSIEFLRDEQALGNTRGASVESLMKPLWAAYQDPNKKMALM
                     QAVATQYLEGNIVVEPRELFVQFALANGINKILT"
     gene            426..1301
                     /gene="rfbA"
                     /locus_tag="PKK85_05120"
     CDS             426..1301
                     /gene="rfbA"
                     /locus_tag="PKK85_05120"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNS25434.1"
                     /translation="MKGIVLAGGSGTRLYPITKAISKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIISTPRDISAYQELLGDGSDLGVSFQYKVQDHPRGLADAFIVGEEFIGNDKV
                     ALVLGDNIFHGHRFSEILERAASLEEGAVVFGYYVKNPHAFGVVEFDREGNVLSLEEK
                     PEHPKSNYVVPGLYFYDNQVVEIAKNVKPSHRGEIEITSVNEAYLERKQLKVELLGRG
                     MAWLDTGTHIGLLEASNFIEAIQKRQGFYIACLEEVAYHNGWIGEDKVLEIAKGLEKT
                     DYAKYLNDLVTDEPL"
     gene            1317..1874
                     /gene="rfbC"
                     /locus_tag="PKK85_05125"
     CDS             1317..1874
                     /gene="rfbC"
                     /locus_tag="PKK85_05125"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNS25435.1"
                     /translation="MGKFTFTKTRIPDVYIIEPTVFGDDRGYFMETYHAQEFKEAGLD
                     FNFVQDNQSKSRRGVLRGLHFQYQHPQGKLVRVIKGEVFDVAVDLRKDSPTYGQWEGV
                     TLSEENRKQFYVPEGFAHGFLVLSEEAEFTYKCTDFYQADDEGGLPWDDPDIGIDWPL
                     DRVDEVLLSEKDQTWKSLEENRVEF"
     gene            complement(1952..>2584)
                     /locus_tag="PKK85_05130"
     CDS             complement(1952..>2584)
                     /locus_tag="PKK85_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25436.1"
                     /translation="SSFQDEIPVNVMDVAGGLMYRDFITVGLLVDSMKISNETSQKTI
                     NDIVPDNWIYIQERDVKLGRLQIFNNWSPYLVSDPDKIWIGLEYFCNEGDEMWNMEDV
                     DFTSFAISELSKIDIIDEDAVKDSVVIRVPKTYPAYFGSYHRFSEIREFTDQYKNLFL
                     IGRNGMHRYNNMDHSMLTAMLAVENIINGVDSKDNLWSINAEEEYHEEKN"
BASE COUNT          699 a          634 c          647 g          604 t
ORIGIN      
        1 ctgctccacg actccttctg gtacaacaac accgcccaga atgccaaact ggcccaggag
       61 attgtggaat acaacaccgg actccacagt gacgccgtgg tggtattcac gccccaagca
      121 gtggacgcca tgatcgcctc ggtaggtggt gtttacattg aaggtgtggg tacggtgaag
      181 ggaaattcca tcgaattcct ccgggatgaa caggccctgg gcaacaccag gggcgccagt
      241 gtagagtcac tgatgaaacc cctctgggcg gcttatcagg accccaacaa gaaaatggcc
      301 ctcatgcaag ccgtggccac ccagtacctg gagggaaata tagtggtgga acccagggaa
      361 ctgttcgtac agttcgcact ggccaatggt ataaacaaaa tattaacata aaggaagtgt
      421 aatgtgtgaa gggaatcgtc ctggctggag ggtccggcac ccgcctgtac cccatcacca
      481 aggccatttc aaagcagtta ctccccatct acgacaagcc catgatctac taccccctgt
      541 cggtgctgat gctagccggg atcagggaaa tactcatcat atccaccccc cgggatatat
      601 cagcctacca ggaactactc ggtgatggca gtgacctggg agtgtccttc caatacaagg
      661 tacaggacca tccccgggga ctggccgatg ccttcatagt aggggaggag ttcattggaa
      721 atgacaaggt ggccctggta ctgggagata acatcttcca cggacaccgc ttcagcgaga
      781 tcctggaaag agccgcctcc ctggaggaag gggctgttgt gtttggttac tacgttaaaa
      841 acccccatgc ctttggagtg gtggaattcg acagggaagg taacgtcctc tccctggagg
      901 aaaaacctga acaccccaaa tccaactacg tggtaccggg actctacttc tacgacaacc
      961 aggtggtgga gatcgccaag aacgtcaaac cctcccatcg gggcgagatc gagatcacct
     1021 cggtcaacga ggcctaccta gaaaggaaac aactcaaagt ggaacttcta ggccggggaa
     1081 tggcctggct ggacaccgga acccacatcg gactcctgga ggcaagtaac ttcatagaag
     1141 ccatccagaa aaggcaaggc ttctacatag cgtgcttgga ggaggttgcc taccacaatg
     1201 gctggatcgg agaggacaag gtcctggaga tagccaaggg cctggagaag actgattacg
     1261 ccaaatacct taatgacctg gttacagatg aaccactcta ggggggactg ctttttatgg
     1321 gaaaattcac cttcactaaa acaagaatac cagatgtgta cataatagaa cccacagtat
     1381 tcggggatga ccggggctac ttcatggaaa cctaccatgc ccaggaattt aaagaagcag
     1441 ggctggactt taacttcgta caggacaacc agtccaaatc ccggagggga gtgttaaggg
     1501 gactgcactt ccagtaccag cacccccagg gcaaactggt ccgggtcatc aaaggggagg
     1561 tcttcgatgt ggccgtggat ctccgcaagg actcacccac ctatggccag tgggaggggg
     1621 tgacattatc cgaggagaat aggaaacagt tctacgtccc ggaaggattt gcccatggtt
     1681 ttttagtctt atctgaggag gcagaattca cctacaaatg caccgacttc taccaggccg
     1741 atgatgaggg aggactcccc tgggacgacc cagatattgg tatagactgg cccctggacc
     1801 gggtggatga ggtgctccta tcagagaagg accagacatg gaagagtctg gaagagaacc
     1861 gggtggagtt ttaggataaa aaagtttacc atcctttcct tttcaataga taagggttat
     1921 caggacacta ttttaactaa gagtgttata actaattttt ctcttcgtgg tactcttcct
     1981 cggcatttat agaccataag ttgtccttgg aatcaacacc attgatgatg ttctccaccg
     2041 ccagcatggc agtaagcatg gagtggtcca tgttgttgta acggtgcatt ccgtttcttc
     2101 caattaaaaa taagttttta tattggtctg tgaattctct tatctcacta aaacgatggt
     2161 aactgccaaa gtaggctgga taggtcttgg gtacgcggat tactacactg tcttttacag
     2221 catcttcatc tatgatatct attttggaaa gttctgaaat agcgaagctg gtgaaatcca
     2281 catcctccat gttccacatt tcatcacctt cgttacagaa atattctagt ccaatccaaa
     2341 ttttatctgg atcagaaact aagtagggac tccagttatt gaatatttgc agtctcccta
     2401 gcttcacatc cctttcctgg atgtagatcc agttatcggg aacaatgtca ttgattgttt
     2461 tctgtgatgt ttcattactg atcttcatgg agtccaccag caaacccacc gttatgaagt
     2521 cacgatacat gagccccccg gcaacatcca tgacatttac ggggatttca tcctggaatg
     2581 aact
//
LOCUS       DAOQVW010000204         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_48198_length_2583_cov_4.280459, whole genome shotgun sequence.
ACCESSION   DAOQVW010000204 DAOQVW010000000
VERSION     DAOQVW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48198_length_2583_cov_4.280459"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..500
                     /locus_tag="PKK85_05135"
     CDS             105..500
                     /locus_tag="PKK85_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25437.1"
                     /translation="MGYLICDICEGYYELQPEEKWEILIKCRCGGKLSWVAKLPTQYQ
                     EENIPDKKDEKATNLFPCHDCGHKISRTAKSCLNCGYKLIYTDQHGKPQSTIETLLYL
                     SAGAFIYSYFIGENAWLYWVIVVVIVNYR"
     gene            complement(771..2396)
                     /locus_tag="PKK85_05140"
     CDS             complement(771..2396)
                     /locus_tag="PKK85_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HNS25438.1"
                     /translation="MICLGLEGTAEKTGAGIVDDEGNILSSKGKQLIPDSGGIHPREA
                     AEHHAASLPQLISEALEEAQLDLYDVDLVAFSMGPGLGPALRTVATAARTLSLRLGVP
                     ILGVNHCVGHVEIGRLTTGCEDPITLYVSGGNSQVIAFDSGSYQVFGETLDIAAGNML
                     DQFARQTGLGHPGGPAVEKLARRSGNYIKLPYTVKGMDLAFSGLLTAALRKYENGAYM
                     EDVCFSLQETAFAMLVEITERALAHARKSEVLLVGGVAANQRLREMVMIMAQEHYAQF
                     FNPPLEYCGDNGAMIAWLGQLMYEHGARQEINDTMVIQRYRTDQVPVPWMKKAGALIK
                     LPPELVAKGAEANIYQDQYLDYPVLVKKRIPKSYRIKEIDKFLRKTRTKGESKLLNQA
                     KRCGVITPLVYDVDVKENTITMEKVVGKLVKDVFYDSRLNEIIKVCHEIGESIARLHQ
                     CGIIHGDLTTSNLILSGDKIVFIDFGLSRISQLTEDKGVDLLVFKKALKGIHHDISQE
                     CFDAILEGYRVADDYQLVVDKIREVEGRRRYVD"
BASE COUNT          695 a          585 c          526 g          777 t
ORIGIN      
        1 gtgcgagaat gtgtaagaat actgcaagat atttttcatt ttttttagtc agtaaaaaga
       61 ggtaaattta aggtaaattg taaaataatc atagaggggc ttctatgggt tatttaatct
      121 gtgatatatg cgagggatat tatgaattgc aacctgagga aaaatgggag attttgatta
      181 aatgtagatg tggaggaaag ttaagttggg tagccaaact tccaacacaa tatcaagagg
      241 aaaatatccc agacaaaaaa gatgaaaaag caactaattt atttccctgc catgattgtg
      301 gtcataaaat aagcagaaca gctaaatcct gccttaactg tggttataaa ttaatatata
      361 cagatcaaca tggaaaacct caatcaacta ttgaaacttt attatatctt agtgctggag
      421 ctttcatcta ttcatatttc attggagaaa atgcatggtt atactgggta atcgtggttg
      481 taatcgtaaa ttatcgatag attgacagat aagagaaaaa aagattagtt tcgttactgg
      541 acgcattgga ctatgatgtt cataacaatt tttctcccaa gtaggcaatc tcagacattt
      601 tcacttggtg attggtaacc atgaaccact gcagaagatt tttccaataa aaacatgggt
      661 tgaaagaatc ccccatctct ttatataatg tttcgagtgt tgccgaagct taaattaagt
      721 tgttttaatt caactgcttg aatctctggc tcatgggctt tttttatcat ctaatctaca
      781 tatcgccttc tgccttccac ttcccgtatc ttatccacaa ccaactggta atcatcagcc
      841 accctgtatc cttccagaat cgcgtcgaag cactcttggc ttatatcatg gtgaattccc
      901 ttcagtgcct ttttaaaaac cagaagatcc actcctttat cttcagttaa ttgggagatc
      961 ctgctcaggc caaagtcaat gaaaactatt ttatctccac ttaaaatgag gttgctggtg
     1021 gtcaggtcgc cgtgtattat tccacattgg tgcagccggg caatggactc tccaatctca
     1081 tggcacacct tgataatttc attcagcctg gaatcataaa aaacatcctt aaccagttta
     1141 ccaactactt tttccatggt aatagtgttt tccttcacat cgacatcgta aaccaaggga
     1201 gttatcactc cgcaacgttt agcctgattt aaaagcttag attcgccctt tgtccgtgtt
     1261 tttcggagaa atttatctat ttcttttata cggtagcttt tggggattct ttttttaacc
     1321 agcactggat agtcaagata ctgatcctgg tagatgttag cctccgctcc cttggcaacc
     1381 agttctgggg gtaattttat cagggcacca gcctttttca tccagggcac cgggacttgg
     1441 tcggtgcggt agcgctggat aaccatggta tcatttatct cttgtctggc accgtgttca
     1501 tacattaact ggcccagcca ggcaatcatg gctccgttat ccccgcagta ctccaggggg
     1561 ggattgaaaa attgggcgta gtgttcctgg gccatgatca taaccatctc tcttagccgc
     1621 tggttggcgg ctactccgcc caccaggaga acttcacttt ttctggcatg ggccagggcc
     1681 ctttctgtaa tttccaccag catggcaaag gctgtttcct gtaagctgaa gcacacatct
     1741 tccatgtagg ctccattttc atatttcctt aacgcagctg ttaaaagccc tgaaaaggcc
     1801 aggtccatcc ctttcacggt gtagggtagt ttgatataat taccggatct tctggctaac
     1861 ttttcaactg ctggcccccc ggggtggcct aggccagttt gtcgggcgaa ctggtccagc
     1921 atgttaccag cggcgatgtc caatgtctct ccgaagacct ggtaactgcc tgagtcaaag
     1981 gcgatcactt gactgttccc tccactcacg tatagggtga tggggtcttc acagccagtt
     2041 gtcaaacgac cgatctccac gtgtcccacg cagtggttca cacccaggat gggaacacct
     2101 agtcggaggg atagggtacg ggcggctgtg gccacggtgc gcagggcagg ccccaatccc
     2161 ggtcccatgg agaaggccac caaatcaaca tcatacagat ctagctgagc ttcttccagt
     2221 gcttcactta ttagttgtgg taaagatgca gcgtgatgtt ctgctgcttc tctggggtga
     2281 attcccccgg aatcagggat cagctgtttt cccttcgatg acagaatatt cccttcatca
     2341 tccactatac ctgcaccagt cttctcagct gttccttcta aacctagaca tatcaaaaaa
     2401 tcacaccaca gtcctcataa aatcatcatc atgatctatt cacatttggc atccttattt
     2461 tataactatg gatctgactt atgaataata gttggatgga atcaaaaaaa agaattgcac
     2521 caatgtaacc ttccctgttg ccaatcttcc tagtatattt ccccttaata tctgctggta
     2581 acg
//
LOCUS       DAOQVW010000205         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_48572_length_2571_cov_3.234102, whole genome shotgun sequence.
ACCESSION   DAOQVW010000205 DAOQVW010000000
VERSION     DAOQVW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48572_length_2571_cov_3.234102"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKK85_05145"
     CDS             complement(<1..302)
                     /locus_tag="PKK85_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HNS25439.1"
                     /translation="MIHNITYRTLVYGTEDEKRVRIALSNILPFSEPDTKLIEGHHKN
                     QMVILTGKTDKKSDIEVFLQSLENIKTSDKKRILRGLENKIDDKGNLFLRFDKQRA"
     gene            complement(373..918)
                     /locus_tag="PKK85_05150"
     CDS             complement(373..918)
                     /locus_tag="PKK85_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HNS25440.1"
                     /translation="MYKYISEAWNNPDDSYVKELMQKRAPLWRKENVITRIDKPTRID
                     RARSLGYKAKKGYVLVRTRVRRGGRRKSRFTAGRRPKRMGVKKITPEKSIQRIAEERV
                     SRHYPNLEVLNSYWVWEDGKFQFYEVILVDPNHPVIKNDPKINWICQSNKKGRAFRGL
                     TSEGKKNRGLKGRGKGKEKVR"
     gene            complement(1144..2532)
                     /locus_tag="PKK85_05155"
     CDS             complement(1144..2532)
                     /locus_tag="PKK85_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNS25441.1"
                     /translation="MNLKNDKSKDSNLPEMTEGVSLITGDPKRAVIKLSGPLIVAMVL
                     SSMYNLVDAIWVSGLGGNALAAVGFVTPLFMILIGLSNGIGAGATSAISRCIGANDKK
                     GVNNTAVHTIIITIIVSLILTLGLEVFLQPLLFFLGAVNTIDLALDYGRIVFAGTILT
                     IFTGAAYGILRSEGDTKRTMHAMILSAVINIILDPILIYVAGLGIAGAAWATIVSQAL
                     VSGVILYWFFKKKDTYVTLSRESFSPDFKVVKSILGVGLPASVEFLVMSSVVAILNGL
                     LVTVAGTDAVAVYSAGWRVVMMAIIPIIAVGTAVVAVAGVSYGARNTENLFITHNYSI
                     KVGMAVALITSTLTYFFADYISMIFAYSPETAYLAPIIAAFLQVMCVFYIFVPPGVMS
                     GSIFQGMGKGINSLILTVLRQFVFVAVFAYILAIFFGWGQQGVWWGIVAGNTTGSIVA
                     FTWARLYIKRLK"
BASE COUNT          728 a          592 c          502 g          749 t
ORIGIN      
        1 taggctcttt gtttgtcgaa tcttaagaat aagttacctt tatcatcaat tttattttca
       61 aggcccctta aaattctttt tttatctgaa gttttgatat tctcaagact ctgtagaaag
      121 acttcaatgt cacttttttt gtcagtttta cccgtaagaa tgaccatctg atttttatgg
      181 tgtccttcta tgagttttgt atccggttca gaaaagggga ggatgttgga aagtgcaata
      241 cgcactctct tctcatcttc agttccataa actagagtcc ggtaggttat gttgtgtatc
      301 actgaccttc atcccaatat gaggttggat atttcctatg gggcattgtt gataaaggaa
      361 taggtactga tattaccgga ccttttcttt tcctttacct cttcccttta accccctatt
      421 tttctttcct tcactggtga ggcccctgaa agcacggcct tttttgttag attgacaaat
      481 ccagtttatt ttagggtcgt ttttaatgac cggatggtta ggatcaacca gtataacctc
      541 gtagaactgg aattttccat cttcccatac ccagtaggag ttcaaaactt ccagattagg
      601 gtagtggcga gatacacgtt cttcagctat gcgttgaatg gatttctcag gtgtgatctt
      661 ctttacaccc atccgtttag gtctcctacc tgcggtgaaa cgagactttc tccttccacc
      721 acgacgaacc cgtgttctga caagcacata accttttttt gctttgtagc ccagggaacg
      781 cgcccggtca attcgggtgg gtttgtctat tcttgttatg acattttctt ttctccacag
      841 gggagctcgt ttttgcatca gttctttgac gtaagagtcg tcaggattat tccaagcttc
      901 actaatatat ttatacattt atacacctct tttgttcagc tttcgccaca tccacgggat
      961 tatatcccaa aaagagtttc gcttcaattg caacgaatat aattattagg ccaggagtat
     1021 ttaaaaagct ttggactgcg aataatcccc gaaagtacta atttctcctg aggttgtgcc
     1081 atggaatata aaacattccc accatccaac acatttcccc ctctacttag taccatacag
     1141 tgactatttg agtcttttga tgtacagacg agcccaggta aaggccacaa tacttccagt
     1201 ggtgttgccc gccacaatcc cccaccatac tccctgctgt ccccagccaa aaaagatggc
     1261 taagatatag gcgaaaactg ctacaaagac aaactgccgt aaaacagtta aaatgagtga
     1321 attaatccct ttacccattc cctggaatat ggagccagac ataactcctg ggggtacgaa
     1381 tatgtaaaaa acacacatca cttggaggaa agcagcgatt ataggcgcca aataggctgt
     1441 ttctggcgaa taagcaaata tcatagagat atagtcagca aagaagtatg ttaaagtact
     1501 ggtgattaag gccactgcca ttcccacttt tatggagtaa ttgtgggtga tgaaaaggtt
     1561 ttcagtattt ctagccccat aagaaactcc tgccaccgct accactgccg ttcccaccgc
     1621 tatgataggt atgatggcca tcatcaccac tctccaacca gcggaataaa cagccacggc
     1681 atcagttccg gctacagtta ccagcaagcc atttaaaatg gctaccacag aagacattac
     1741 cagaaattcc acactagctg gtaatcctac ccctaaaata gatttaacca ccttaaaatc
     1801 aggtgaaaaa ctctcccggg atagagtgac gtaggtgtcc ttctttttga aaaaccagta
     1861 caagataact ccagatacta gagcttggga tactattgtt gcccaagccg ccccagcaat
     1921 acccagtcct gccacgtaaa tcaggatggg atccagaata atgttgatca ccgcagatag
     1981 tatcatggca tgcatggtcc tcttggtgtc tccctctgaa cgcagaattc cataggccgc
     2041 accagtgaag atagttagta ttgtaccggc aaagacaatt cgaccataat caagagccaa
     2101 gtcaatggtg tttacagctc ccaggaaaaa caggaggggt tggaggaaca cctccaaacc
     2161 cagggttaga ataagcgaaa caatgatggt gattattata gtatgtacag cggtgttgtt
     2221 tacacccttc ttgtcattgg cacctataca acgggaaatg gcagatgtgg ccccagctcc
     2281 aataccattg gaaaggccga tcagtatcat gaatagtggg gttacaaaac ccactgctgc
     2341 cagagcattt cctcctaacc cagaaaccca gatggcatcc accagattgt acatggatga
     2401 taaaaccatt gccacaatta ggggtccgga taattttata actgcccttt tggggtcgcc
     2461 tgtgattaga gaaaccccct cagtcatttc agggagatta gaatctttag atttatcgtt
     2521 tttcaagttc attaccttaa aatttaaatt agaatggtta catccttcaa a
//
LOCUS       DAOQVW010000206         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_48659_length_2568_cov_3.148428, whole genome shotgun sequence.
ACCESSION   DAOQVW010000206 DAOQVW010000000
VERSION     DAOQVW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48659_length_2568_cov_3.148428"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKK85_05160"
     CDS             <1..500
                     /locus_tag="PKK85_05160"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNS25442.1"
                     /translation="MPAFGDGYQMHITGLTHDERGYPDASSPQAHFTLVKRLCDKILE
                     HTDDIGRIKSFYTEDAEVVVLAYGVPARPGLKSVRMARDQGLKVGFIKLDTVWPFPEN
                     AILESLKGVNRVIVAEMNLGQIFYEVQRILPGVKVEKASKIGGEMHLPQEILRLIKSD
                     GRSGG"
     gene            504..1379
                     /locus_tag="PKK85_05165"
     CDS             504..1379
                     /locus_tag="PKK85_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNS25443.1"
                     /translation="MEKTRENRFIKYLRKDRLPNIFCAGCGNGIVMNTFFNGMEMAQI
                     SMEDVVMVSGIGCSSRIPGYVKCDSLHTTHGRPISFATGLKLANPDMNVVVFSGDGDT
                     AAIGGNHLIHGARRNMDLTVICINNSIYGMTGGQISPTSPHGSYGSTAPYGSLENPFD
                     LSKLVTAAGASYVARWTTAHPLQLSSAIKKGLKNRGFSFIEVVSQCPTYFGRKNRMRS
                     PIDMMRWMKEESVVKRKADGMDESELVGKIIVGDFHKKEAPEFTERICQLSSEQCPPE
                     KPSAIRSAYRGDSSA"
     gene            1372..1923
                     /locus_tag="PKK85_05170"
     CDS             1372..1923
                     /locus_tag="PKK85_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HNS25444.1"
                     /translation="MRKEIRIAGFGGQGIILAGIVIGKAATLYDGLYAVQTQSYGPEA
                     RGGASRTEIIISDVEIDYPKVQRPDILVAMSHEALIQYMDDLKDGGILIIDPDLTMEE
                     EILPFVREHDIKLHRAPATRTAEEEIGLRIVANIVMIGAITGITKAITLEAARTAVKV
                     SVPPGTEKKNLAAFEAGFKLAKG"
     gene            1929..>2568
                     /locus_tag="PKK85_05175"
     CDS             1929..>2568
                     /locus_tag="PKK85_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNS25445.1"
                     /translation="MKIYEYQAKEIFKQEGIAIPRSKMVKNATDARQAAEEIGAPVAV
                     KSQVLVGGRGKAGGIKFANNPEEAYEAAQKLLKMDIKGEMVYKLLIEEKLAIESEYYI
                     SVALDRSARQALIMASTAGGVDIEEVALRNPEQIFKVHVDPRQEFLPFLARDIARKMG
                     VEGPLIPALGAVIFKIYRIFQKYDANLVEINPLVATSDGLVAADAKLDVDDDS"
BASE COUNT          761 a          525 c          674 g          608 t
ORIGIN      
        1 ccatgcccgc ttttggagat ggatatcaaa tgcacataac tggactaacc catgatgaac
       61 gggggtaccc cgatgcatcc agtccccaag cccactttac cctggtgaaa cggctttgtg
      121 ataagatctt ggaacacacc gatgatattg gtcgtattaa aagtttttac accgaagatg
      181 ccgaggtggt ggtcctagct tacggagttc cggcccgacc cggtttgaag tcggtgagaa
      241 tggctcgtga ccagggactt aaagttggct tcattaaact ggacacggtg tggcccttcc
      301 cagaaaatgc tattttagag agtttaaaag gtgtaaatag ggttatagtg gcggagatga
      361 acttaggtca gatattttac gaagtacaga ggattctacc aggagtgaag gtggaaaagg
      421 cttcaaaaat tggtggagaa atgcatcttc cccaggaaat actgcgcctc ataaaatcag
      481 acggaagatc gggaggatag aggatggaga aaacaagaga aaatcgattt ataaaatatc
      541 taagaaagga tcgcctgcct aacatcttct gtgctggttg tgggaatggc atcgtgatga
      601 acaccttctt caatggcatg gaaatggcac agatcagtat ggaagatgtg gtcatggtat
      661 ctggaattgg ctgttcctct cgaatacctg gctacgtgaa gtgtgattca ctacatacca
      721 ctcatggtcg gcctatttca ttcgccactg gattaaagct tgctaatccg gatatgaatg
      781 tggtggtctt ttcgggtgat ggagacacag ctgccattgg tggaaaccat cttattcacg
      841 gggcccgtag aaacatggat ctcacggtga tctgtataaa caacagtatc tacgggatga
      901 ccgggggaca gataagtccc accagtcccc atggtagtta cgggagcact gcaccctatg
      961 gatctctgga aaatcctttt gatctttcta aattggttac tgcagcaggg gccagttatg
     1021 tggcccgctg gaccactgca catcccctac agctttccag cgccataaaa aaagggctga
     1081 aaaaccgagg attctccttt attgaggtgg tttctcagtg ccccacctat ttcggacgta
     1141 aaaacaggat gagatccccc atagatatga tgcgctggat gaaggaagaa agtgttgtca
     1201 agaggaaagc ggatgggatg gatgaatcag aactcgtagg taaaataata gtgggggatt
     1261 tccataaaaa agaggctcca gagtttactg aaagaatctg ccaactttca agtgaacaat
     1321 gccccccaga aaaaccatcg gcgattcgat cagcataccg gggtgattcg agtgcgtaaa
     1381 gagataagaa tcgctggatt cggcggtcaa ggtataatat tggctggaat agttatagga
     1441 aaagcggcaa cactttacga tggtctctac gccgttcaaa cccaatcata tggtccggaa
     1501 gctagagggg gggcttcccg aacggagata ataattagtg atgtggaaat agattatccc
     1561 aaagtccaaa gacctgatat cctggtggcc atgtcccatg aggccctaat acaatacatg
     1621 gacgacttga aggatggagg tattctgatc atcgacccgg accttacaat ggaggaagaa
     1681 atacttccct ttgtacggga gcatgacata aaacttcacc gggcaccagc cacccgcact
     1741 gccgaagaag agatcggcct gcgcatagtg gccaacatcg tcatgattgg agctataact
     1801 ggtattacta aggccattac tctagaagca gctagaactg cggttaaagt cagtgtgccg
     1861 cctggaactg agaagaagaa tctagctgcc tttgaagcag gctttaagct agctaaggga
     1921 tgattggaat gaaaatttat gaataccagg ctaaggagat attcaaacaa gaaggcatag
     1981 ccatcccacg aagtaagatg gtgaaaaacg caacggatgc tcgtcaggct gctgaagaga
     2041 taggagcgcc ggtggcagtt aagtcccagg tactggtagg aggaagaggc aaagctggtg
     2101 gtataaaatt tgccaacaac cccgaagagg catatgaagc tgctcaaaaa cttctgaaaa
     2161 tggatataaa gggtgaaatg gtctataaac tattgataga ggaaaaattg gccattgaat
     2221 ctgaatatta tattagtgta gctctggatc gctcagctag acaagccctg atcatggcca
     2281 gtactgcagg gggggtggac atcgaagaag tagcccttcg aaaccctgaa cagattttta
     2341 aagtacatgt ggatcccagg caggaatttt taccatttct ggcccgggat atcgcccgca
     2401 agatgggggt agaggggcca ctcatcccag cattaggggc cgttatcttc aaaatttaca
     2461 ggatattcca aaaatatgat gctaaccttg tagaaattaa ccccctggtg gctactagtg
     2521 atgggctggt ggcagctgac gccaagttgg atgtggatga tgactctc
//
LOCUS       DAOQVW010000207         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_48842_length_2562_cov_3.437176, whole genome shotgun sequence.
ACCESSION   DAOQVW010000207 DAOQVW010000000
VERSION     DAOQVW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48842_length_2562_cov_3.437176"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PKK85_05180"
     CDS             <1..669
                     /locus_tag="PKK85_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate-mannose-protein
                     mannosyltransferase"
                     /protein_id="HNS25446.1"
                     /translation="KISIEPTSRNLIIWKNVTENKSFDIRGPYLVDSALENESSVIRS
                     PPDAMSSLGLGYEDVIYINQRRYNLSQNLPGEENVLRIPYDAREYFGLNIRDIIPDTN
                     LRNVFKNLYAERNLILQNAGYLTIKEGVILRSGEEDGKNINIYLNDQIISSTTGIINN
                     GTYSIYVNGVKYREVFINKDKPTTTIYIQSRLDTVKIVVEGNVKSDMEIPTAGRGKFL
                     NVIF"
     gene            complement(785..1717)
                     /locus_tag="PKK85_05185"
     CDS             complement(785..1717)
                     /locus_tag="PKK85_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1743 domain-containing protein"
                     /protein_id="HNS25447.1"
                     /translation="MEILTSQDLKERFQDPWIVPYQKVLTLVDDDLVEIVEYHPCIGG
                     SEWMVYQYGRSSELVIKSERDGNRHTYLTRIGKVNLNLKPSFRAAGIEEVAVEEDEVR
                     VVHAGLAGAGVGAGMCRGMAEGVKRIELYDVGGGSKLGRAAVITPRLEKVVLGIDDTD
                     TKEEGATWTMAHNVAMELAKKGYQYIDHITVQLYPHNPHKTQNCVAIALVFAVKPGER
                     ENLVKEACQLLKNYTLSDKTAIAVLSGIRVPEKLRDYSEATKKSMITVEEAEKVAKEI
                     GVELVEVTGKQGKIGALAALGVYNDLKEAARVYE"
     gene            complement(1890..2300)
                     /gene="ribH"
                     /locus_tag="PKK85_05190"
     CDS             complement(1890..2300)
                     /gene="ribH"
                     /locus_tag="PKK85_05190"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNS25448.1"
                     /translation="MVKVRIGAVVAEFNYDITHMMLELAKEHAKFLDSEITEVITVPG
                     VFDMPLAIKKLLEMDDIDAAITLGAVIEGATDHDQIVVQHASRKIADLALDFDKPVAL
                     GITGPGMTRLEAHQRVDYAKRAVEAAVKMVERLK"
BASE COUNT          732 a          609 c          461 g          760 t
ORIGIN      
        1 aagatatcta tagaacctac atctcgtaac ctgattattt ggaaaaacgt caccgaaaac
       61 aagagttttg acatccgcgg cccttacctg gtggatagtg ccttggaaaa tgaatcttca
      121 gtgatcagaa gccctccaga tgcaatgagc tccttgggcc tgggatacga agacgttatt
      181 tacattaacc aacggcggta taatttgagt cagaacctcc cgggggaaga aaatgtcctg
      241 cgcataccct atgatgcacg tgaatacttt ggtttgaaca tccgggacat cattcccgac
      301 accaatttaa ggaacgtctt caaaaatcta tacgctgaaa gaaacctaat tctccagaat
      361 gcaggatatt taactattaa agaaggagta atcctgcgaa gtggagagga agatggtaaa
      421 aacattaaca tctacctcaa cgaccaaatt atcagttcca ccacaggaat aatcaacaac
      481 ggaacctaca gcatctatgt gaacggcgtt aaataccgag aagttttcat taacaaagac
      541 aaaccaacca cgaccattta cattcaaagt agactagaca ctgtgaaaat agtagttgaa
      601 ggtaatgtga agtctgacat ggaaatcccc acagcaggta ggggaaaatt tctgaacgta
      661 atattctagg ccatcagatt aatagtaggc cattggaagg gcagtgccgg tgggataaac
      721 tattccccaa gcctaccgtg aagatgaact cagtaaaatc taatccagac catatccagg
      781 gattctattc ataaactctg gcagcttcct tcaaatcatt gtaaacacct aaagcagcta
      841 gggcccctat ttttccctgt ttacccgtaa cctccaccaa ctccactcca atttctttgg
      901 caactttttc tgcctcttcc actgtaatca tggatttttt tgtagcttca gaataatctc
      961 taagtttttc aggtactcta attccagaaa gtacagcaat tgccgtttta tcagataaag
     1021 tgtaattttt taatagttgg catgcttctt taaccaaatt ttctctttca ccaggtttaa
     1081 ccgcgaagac cagagctata gccacacaat tctgggtttt atgggggtta tgcggataaa
     1141 gttgtaccgt gatatgatcg atatactgat aacccttttt tgcaagctcc atggcaacat
     1201 tatgcgccat agtccaggtt gccccttctt ctttggtgtc agtatcatct atgcctagaa
     1261 ctaccttttc caagcgaggg gttatcactg cggccctgcc tagcttggat cctccaccca
     1321 catcgtaaag ttcaatcctt ttaactcctt cagccatccc gcgacacatc cccgccccca
     1381 ctcctgcacc ggcgaggccg gcatgaacca cccgtacttc gtcttcttca actgcaactt
     1441 cttcgattcc tgcagcccga aaacttggtt taagatttaa attcactttg cctatcctgg
     1501 ttaggtaggt gtgccggttt ccatctcttt ctgatttgat taccagttca ctggatcttc
     1561 catattggta gaccatccat tccgatcccc caatacaggg gtggtactcc acaatttcca
     1621 ccaagtcatc atccaccagg gttagtactt tctgataagg gactatccag gggtcctgaa
     1681 acctttcttt aaggtcttgt gaggttaata tttccatgct gatctttcca tcggtattta
     1741 aattttcttc aatatgctgc tacagccgga tcggccataa aatactacac cataatataa
     1801 taataatttg ctaagatgat tgccctatgg cgatttgtgg taaattagat ttgtgaaata
     1861 aggaatatta aaaaaaaaca agtggatgtc tacttcagtc gctccaccat tttaactgca
     1921 gcttcaacgg cacgtttagc ataatccact ctttgatggg cttctaggcg ggtcataccc
     1981 ggaccagtga tgcccagggc cactggttta tcaaaatcca gggctaaatc agcgattttt
     2041 cgcgacgcat gctgcactac gatctggtcg tggtcagtag caccttcaat aactgcaccc
     2101 agagttatag ctgcatcgat gtcatccatt tctaaaagtt tcttaatggc caatggcatg
     2161 tcaaaaaccc ccggaacagt tattacttca gttatttctg aatctagaaa tttagcgtgt
     2221 tcttttgcga gttctaacat catgtgggtt atgtcatagt tgaattcagc gacaacggcc
     2281 cctatcctta ccttaaccat gcttggttcc ctcccttact caattaccta ccttcaatct
     2341 caaataagaa ttatttcgat tcttatattt aatttgtcga ttctttagaa taatccatta
     2401 gaatgataaa acaattccag ccatcaaact aatgatcata atcactacga tgaacagagc
     2461 tattatctgt ggacgttcca ttttttgtct cctttttctt agctatctcc caaaattgga
     2521 tatttcttcc agacaatcac ccagaacttt tatatcttcc ag
//
LOCUS       DAOQVW010000208         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_49039_length_2555_cov_3.477200, whole genome shotgun sequence.
ACCESSION   DAOQVW010000208 DAOQVW010000000
VERSION     DAOQVW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49039_length_2555_cov_3.477200"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PKK85_05195"
     CDS             <1..173
                     /locus_tag="PKK85_05195"
                     /inference="COORDINATES: protein motif:HMM:NF021440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HNS25449.1"
                     /translation="EERQQTMEAMKRVAEIEMGKELNQTPQTKSGSGEGFVQKGTVYQ
                     DPQSGKLIMVEG"
     gene            267..692
                     /locus_tag="PKK85_05200"
     CDS             267..692
                     /locus_tag="PKK85_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25450.1"
                     /translation="MKLKLIRYTTGVVLIKNLEDRYGSRDNLKTLIKKDPENFLYHLD
                     WETWQHHQENPRALVEESETIFLRNQQIGLHHLNLLKVIKKEKPNSIDNLSSILNQDP
                     EEVKKQVQILAQEGLLKLVPGPDGDEIPLVDFKKIEIEI"
     gene            complement(778..1566)
                     /gene="cbiQ"
                     /locus_tag="PKK85_05205"
     CDS             complement(778..1566)
                     /gene="cbiQ"
                     /locus_tag="PKK85_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNS25451.1"
                     /translation="MEGISEIDREAQKESFIHNLDGRIKLISAMLIIIYAVSSTSIVI
                     LAIMELYLLILISISNITPKYAIKRIALIIPFGGLIALIQPFFQPGTVIWTGPFGWLH
                     ITDYGLFFGALLLSRMVVAITAIVFLSSSTSMQDLVASAQRLGVPHQLAMLLNLTVRY
                     LFFFYDQLENILNAQSTRCFDIFSNKTSYKWRLRKVGETITMMFLRAFEQGENVYLSM
                     MSRGYSENTRTYRAKTSLDSKDYMFFSGTLLVILSLQLANMFVL"
     gene            complement(1641..1940)
                     /locus_tag="PKK85_05210"
     CDS             complement(1641..1940)
                     /locus_tag="PKK85_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HNS25452.1"
                     /translation="MNTKNRNLVIGGLAIAIVIAILAPFLASSNPDGLESAAESLGVP
                     ESEAVFESPLPDYAIPGMEDNPWGGVIALIFGTILVVLVMLGLAYIISKKGNGQE"
     gene            complement(1937..>2555)
                     /gene="cbiM"
                     /locus_tag="PKK85_05215"
     CDS             complement(1937..>2555)
                     /gene="cbiM"
                     /locus_tag="PKK85_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HNS25453.1"
                     /translation="WQCAIYWFIAIVAIGFALRWARENLDEKNIPLLAVLAAGIFAIQ
                     AMNVPIPWGTSGHMVGAALIAIIFFSPWAAVLVLSIVLILQGLIFGDGGMTALGANIV
                     NMGLIGGFVGFYAYKVLKGVGRIPAIFLAGWASIFLAAIACAIEMAWAGTFPLAEGIV
                     FMGLYHAVIGVIEGVISVVVILGIDNVRPDLLADWVSSSKKEALQ"
BASE COUNT          801 a          561 c          538 g          655 t
ORIGIN      
        1 ctgaggaacg tcaacaaacc atggaggcca tgaaacgggt agcagaaata gagatgggga
       61 aagaacttaa tcaaacaccc cagaccaagt ccggatccgg tgaaggcttt gtacagaaag
      121 gaacagttta tcaagacccc caaagtggaa aactaatcat ggtggagggt tgatcgatat
      181 ttacggagtg tccaggggga tgctctgtaa attaaaccca tactttatga agaacaaagt
      241 gtctcagtgg atagataagg aataccatga aactgaaact catccgttac accacggggg
      301 tagtattgat taagaacctg gaagacagat acggctcccg agataatttg aaaacactga
      361 taaaaaagga tcctgaaaat tttttgtacc acttagactg ggaaacttgg caacatcacc
      421 aggaaaatcc ccgggcattg gtggaagaaa gtgaaaccat cttcctccgt aaccaacaga
      481 taggcttgca ccacctgaat ttactgaagg ttatcaaaaa agagaaacct aattccatag
      541 ataacttgtc ctctatcctc aaccaggatc cagaggaagt taaaaagcaa gtacagatat
      601 tagcccagga aggtctgtta aaattggtac ctggtcctga tggggatgaa atacccctgg
      661 ttgattttaa aaaaatagag atcgagattt gatgttacat cactaatttt ttcacactcg
      721 gagttatcta aatatttaca aactgttact tcttctggat tgaggttgtg aggcaaatta
      781 aagaacgaac atattcgcca gctgcagact cagtataacc aacagggttc cgctgaaaaa
      841 catataatcc tttgaatcca ggctagtttt ggcacggtaa gtccgggtat tctcggaata
      901 accacgggac atcatactca gataaacgtt ttcaccctgt tcaaaggccc gaagaaacat
      961 catagtaatc gtttctccta ctttccttaa tctccactta taagaggttt tgttactaaa
     1021 tatatcaaag caacgggtgg actgggcatt taaaatattc tcaagttgat cgtagaagaa
     1081 aaacaggtac ctaaccgtaa ggttcaggag catggccaat tggtggggta ctcctagtct
     1141 ctgtgcagag gccaccagat cctgcatgga agtggatgat gataaaaaga caatggccgt
     1201 aattgccact accattctgg ataggagtaa agctccgaaa aacaagccat aatctgtaat
     1261 atgcagccaa ccaaaaggcc cagtccagat cacagttcct ggttggaaga atggttgaat
     1321 taaggctatt aatcctccga atggtatgat gagggctatt cgttttatgg catacttggg
     1381 agttatattg gatatggaaa tcagaattag aaggtacaac tccattatgg ccagaatcac
     1441 gatgctggtg ctggaaactg catatattat gattaacatt gcagatatga gctttattcg
     1501 tccgtccagg ttgtgtatga agctttcttt ctgggcttcc cgatcgatct ctgatattcc
     1561 ttccataaat atcttccatt agttttttga taaaaaaatt agaaaaaaat ggataggagc
     1621 taaaaaagct ccatttaggg ttattcttgg ccattacctt ttttactgat gatataagcc
     1681 aatcccagca ttactagcac aactaatatc gtcccgaata tgagggctat taccccgccc
     1741 cagggattgt cttccatgcc aggtatggcg tagtccggta atggtgattc gaacactgct
     1801 tcggattctg ggactcccaa actttccgcg gcactttcca gtccatcggg attgcttgag
     1861 gccaaaaaag gtgctaagat ggctataact atggctatgg ccaggccacc aatcaccagg
     1921 ttacgattct tggtgttcat tgcaatgcct cctttttact gctactaacc cagtcagcta
     1981 acaaatcggg acgcacattg tcaattccca ggatcaccac aacagagatc acaccttcaa
     2041 tgacaccaat cacagcgtga taaagcccca taaaaacaat tccttctgcc agtgggaaag
     2101 taccggccca agccatttct atggcacatg ctatggctgc caggaatatg gatgcccatc
     2161 cagcaaggaa gattgctggt attcttccaa ctccttttaa aactttgtag gcatagaatc
     2221 caacgaaacc gccaataagc cccatgttaa ctatgttagc tcctaaggct gtcattccac
     2281 cgtcgccgaa tattaaaccc tgtaaaatca gtacaatgga taaaaccagt actgcagccc
     2341 agggactgaa aaatataatt gctatcaacg ctgcgcctac catatgacca ctggttcccc
     2401 agggtatggg tacgttcatg gcttgaatgg cgaatatacc tgcagccaga acagccaaga
     2461 ggggtatgtt cttctcatcc aggttctccc tggcccatct aagagcaaaa cctatagcta
     2521 caatggcaat gaaccagtag atcgcacact gccat
//
LOCUS       DAOQVW010000209         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_49421_length_2543_cov_3.438907, whole genome shotgun sequence.
ACCESSION   DAOQVW010000209 DAOQVW010000000
VERSION     DAOQVW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49421_length_2543_cov_3.438907"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKK85_05220"
     CDS             <1..195
                     /locus_tag="PKK85_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNS25454.1"
                     /translation="HTHYAFINQMMGLLLLNPGSPTVPRLSDPSVMLLEVDDGEVEAE
                     LIKIGDSTCKALNYQGRGIQ"
     gene            250..861
                     /locus_tag="PKK85_05225"
     CDS             250..861
                     /locus_tag="PKK85_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNS25455.1"
                     /translation="MSKRWNQERKEDLYYQKAKKEDYRSRASYKLLQLNKRFKIIRKG
                     DRILDLGAAPGGWSQVALEAVGEEGLVVAVDLQRIRPFVEDNFKAIKGDFTTADVKKD
                     IISALGGPVAVVISDASPKLSGIKDVDQMRSMDLFLAVMEICDSALRYNGSLLMKLFQ
                     GPQFPEILKVVKRKFRNVKTTKPPSSRKKSVEMYLVARGFYGL"
     gene            complement(886..1320)
                     /locus_tag="PKK85_05230"
     CDS             complement(886..1320)
                     /locus_tag="PKK85_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25456.1"
                     /translation="MKRSRLNLFKATSMTISVLGVVMIIITVVIVAYLGFQSLSSIIS
                     SDASSGKYYEQLTDLKSDYSNLKAQFDSTKTSVDTSGKKDLKEAYINAELELVKAQSA
                     INDAESAISAGKSSEEIQNRLNTARTQLQDAKSSLSNVRGML"
     gene            1655..>2543
                     /locus_tag="PKK85_05235"
     CDS             1655..>2543
                     /locus_tag="PKK85_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS25457.1"
                     /translation="MEISAQETTNKTKTSIAKFEEFFGTKYKDTVFEALEKYPDERSV
                     VVDYNELEMFDPDLADLLIEKPEEVLKAAQLAIQNIDPLRKNAELHTRFQNLRNLIPL
                     RDLRSKYIGKFVAIDGIVRKTDEIRPRIVNATFECRGCMRLHEVSQTSNLISEPALCQ
                     ECGGRSFRLLQEESEFLDTQNTKLQEPLENLSGGEQPRQIVVVLEDDLVDTITPGDIV
                     RITGTLKTVRDEKTKRFKNYIYGNYIEAKEQEFEELEISPEDEEKIVEMSKDPNIYDK
                     IIKSTAPSIQGYRAVKEAIA"
BASE COUNT          753 a          535 c          582 g          673 t
ORIGIN      
        1 cacacccact atgccttcat caatcaaatg atgggcctgc tgcttttgaa cccgggaagc
       61 cctactgtgc cccgactttc agatccatcg gtgatgctcc tggaggtgga tgatggagaa
      121 gtggaggctg aactgattaa aatcggtgac tccacttgta aagccctgaa ctaccaggga
      181 agaggtattc aataagattc attctatttt aaactggtaa catgtcaatt attataaaag
      241 aggaattgga tgagtaaaag atggaatcag gaacggaaag aagatcttta ctaccagaaa
      301 gccaaaaaag aagattaccg ttcccgggct tcctataaac ttcttcagtt aaataaaagg
      361 tttaaaatca tccgtaaagg ggaccgtata ctggatttag gggctgctcc cggtggatgg
      421 tcccaggtgg cactggaagc tgtgggggag gaaggcctgg tggtggcggt ggatctgcaa
      481 aggatccgtc cctttgttga agataatttc aaggccatca agggagattt taccactgca
      541 gacgttaaaa aagatattat atcagcatta ggaggaccag tggcagtggt aatatctgat
      601 gcatccccta aactctcggg tataaaagat gtggatcaga tgcgctccat ggatcttttc
      661 ttggctgtga tggagatttg tgactccgcc ttgcgataca atggtagcct gcttatgaag
      721 ttgtttcagg ggccacaatt cccggagatt ctaaaagtag ttaaaaggaa attcaggaat
      781 gtgaagacta ccaagccgcc ttcgtcacgt aagaagagtg tggagatgta cctggtggcc
      841 cggggcttct acgggttatg aatgaagcat atcatggatt ttcacttata acataccccg
      901 gacattactt aaactagatt tagcatcctg taactgagtt ctagcagtgt taagcctatt
      961 ttgaatttcc tctgatgatt tcccagccga tatagcactt tcagcatcat ttatggccga
     1021 ttgggccttt actaattcta attcagcatt tatataggct tccttcagat cctttttacc
     1081 actggtatcc accgaagtct tggtactatc aaactgggcc tttagattag agtagtctga
     1141 tttcaagtca gttaattgtt cataatactt tccactgctg gcgtcactgg atattattga
     1201 tgataatgac tggaagccga ggtaggctac gatgacaacg gtaattatga tcatgaccac
     1261 tcctaacacc gatatggtca tagaggtagc tttaaagaga tttaaccttg atcttttcat
     1321 atctgattcg cctcatagct aaggtaggtg ataatctaat gattgttttt aattgttcga
     1381 tatcaatagc ttacttttat catatataaa gggaagtatg gcgtttgagc ccctcagagg
     1441 aagtatataa ccatccccta agtcgtcgca gtgatccagt tcagagttaa tctaccacct
     1501 atagatggaa attgtatcaa cagccctata cttcatctag agcaagcatc ctgactccag
     1561 cgtggttaat gtgcctgcat tcaactttct ttacacttga aatctacgtc tccgtctcta
     1621 aatatttata gcaagaggga gtaatgatcg ttcaatggaa atctcagccc aagaaacaac
     1681 taacaagaca aaaacatcca tagccaaatt cgaggagttt ttcggcacta aatataaaga
     1741 caccgtcttt gaagccctgg aaaagtatcc ggatgagcgt tcggtggtgg ttgattacaa
     1801 tgaactggag atgttcgatc cagacctggc ggatcttctg attgagaaac cagaggaagt
     1861 gctaaaagct gctcagttgg ccatacagaa catagatcct ctccgtaaaa atgcggaact
     1921 ccatactcgt tttcagaatc taagaaatct tattcctctg cgggatttaa ggagcaagta
     1981 catcggaaaa ttcgttgcca tcgatggcat cgtgcggaaa accgacgaaa tacggccccg
     2041 cattgtaaat gccacctttg aatgccgcgg ttgcatgcgc ctgcacgaag ttagccagac
     2101 cagtaatcta atcagtgaac cagcactctg tcaagagtgt ggaggacgct ctttccgttt
     2161 actccaggaa gaatccgaat ttctggatac ccaaaacacg aaactccagg aacccctgga
     2221 aaacttgtca ggtggggaac aaccccgtca gatagtggtt gttctggaag atgacctggt
     2281 ggacaccatt accccggggg atattgtcag aatcaccggc acactcaaga cagtccggga
     2341 tgagaagacc aaacgattca aaaactacat ctatgggaac tacatcgagg ccaaggagca
     2401 ggagtttgag gaactggaga taagtcccga ggacgaggaa aaaattgtgg agatgtccaa
     2461 ggatcccaat atttacgata aaataataaa atccactgca ccatccatac agggctaccg
     2521 ggctgttaaa gaggccattg ccc
//
LOCUS       DAOQVW010000210         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_49768_length_2533_cov_2.730831, whole genome shotgun sequence.
ACCESSION   DAOQVW010000210 DAOQVW010000000
VERSION     DAOQVW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49768_length_2533_cov_2.730831"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /gene="cobB"
                     /locus_tag="PKK85_05240"
     CDS             complement(<1..613)
                     /gene="cobB"
                     /locus_tag="PKK85_05240"
                     /EC_number="6.3.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenobyrinic acid a,c-diamide synthase
                     (glutamine-hydrolyzing)"
                     /protein_id="HNS25458.1"
                     /translation="MRLVLAGTGSAVGKTTIATGIMKAFSKDYRVQPYKVGPDYIDPT
                     YHTLATGHDSRNLDSFFMSEPQIREAFQRGFIASKGDMGIIEGVRGLYEGISPTGDVG
                     NTASVAKALNAPVILILNTRSLVKSAAAIVIGFKTLDPDVRIEGVILNRVKNHKHYLK
                     TKEAVEKLAKTPVVGGIPRSDDIKVEQRHLGLVPAMERENILGY"
     gene            709..>1621
                     /locus_tag="PKK85_05245"
     CDS             709..>1621
                     /locus_tag="PKK85_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS25459.1"
                     /translation="MNLEKLRILGDGSRYDLCNYVSLNQENFTKVKQPGIYHTKSSCG
                     SSVPLFKTLMSNHCTSDCLYCVNNCQNKFQRDEYTPQELVLVFLHYYENHYAEGLFLS
                     SGIPGDPDQAMDNLVEVARLLRKEHEYQGYIHLKILPGASYDHIKRAMGLADRISVNL
                     ESATATGFSELTTTKDYQKDVLRRMKWINRLKNRYPQLVPAGQSTQLMVGANQESDAD
                     ILKRVDLLKKNLNIKLSYLSPFTPLDNTPLYNHSQPDPKRVPRLYQAQFLINSYGFPL
                     EEMVLDDSEHLILDSDPKQLWAQLHPEH"
     assembly_gap    1622..1631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1632..1840
                     /locus_tag="PKK85_05250"
     CDS             <1632..1840
                     /locus_tag="PKK85_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNS25460.1"
                     /translation="PVEVNESSFNDLIKVPGIGPKSARKIIAARRAGHVFTTLEDLKG
                     KGVWIKRAEPFIKLNRVYQSTLNF"
     gene            complement(1910..2272)
                     /locus_tag="PKK85_05255"
     CDS             complement(1910..2272)
                     /locus_tag="PKK85_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS25461.1"
                     /translation="MSPKKSGGALGKGLDALIKRDEPETTETRGKPGVKKSVKESKTP
                     NVKAKKDHDPTIQAVLIEVHKNPRISLWSARSAAVLRFLKKTKPEFSISKEASVLIEA
                     AVADKYPDIWRLFQDQDF"
     gene            complement(2269..>2533)
                     /locus_tag="PKK85_05260"
     CDS             complement(2269..>2533)
                     /locus_tag="PKK85_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNS25462.1"
                     /translation="LKSPSPIKGILLTLYDGRTRLGRDVYRNIKEYFGEREYVFKTVI
                     PRNITLAEAPSHGMPCVIYDEESTGTEAYKKLAREFIQLEDNK"
BASE COUNT          703 a          586 c          495 g          739 t
ORIGIN      
        1 tgtatcccag gatattttcc ctttccatag cagggaccag gcccaggtgg cgttgttcaa
       61 cttttatatc atcactacgg ggtatgccac ccaccaccgg tgttttagcc agtttttcca
      121 ctgcttcttt ggttttcaag taatgtttat ggtttttcac ccgattcagg atcactccct
      181 caatacgaac atctggatcc aaggttttaa aacctataac tatggctgca gcacttttaa
      241 ccagactccg tgtatttaat atgagaataa ccggggcatt taaagcctta gccacggatg
      301 cagtgttacc cacatcaccc gtaggactta taccttcata cagaccccgg acaccttcaa
      361 taattcccat atcccccttg gaagctataa accccctctg gaaagcctcc cttatctggg
      421 gttctgacat gaaaaaggag tccaggttcc gggagtcgtg gcccgtggcc agggtgtggt
      481 aggtgggatc aatgtaatcc ggccccactt tatatggttg caccctgtaa tcctttgaaa
      541 acgccttcat tattccagtg gcaatggtag tttttcccac tgcactaccg gtaccagcca
      601 ataccaatcg catgtttttt agtttaccac cctcactata aaaactcata tcccatcttc
      661 ttttctttta aaaaaattta tgatgtaagg gaaaaatgat ataacataat gaacctggaa
      721 aagctgagaa ttctggggga tggatcccgg tacgacctct gcaattatgt tagtcttaat
      781 caggagaatt ttactaaagt caaacaacca ggaatctatc ataccaaaag cagctgtgga
      841 tcttcagtgc cactgtttaa aaccctgatg agcaatcact gcacaagcga ttgtctttac
      901 tgtgttaaca actgtcagaa caagtttcaa cgagatgaat acactccgca agagttggta
      961 ttagtgtttc tccattatta cgagaaccac tacgcagaag gacttttttt aagctccggt
     1021 attccagggg atcctgacca ggccatggac aacttggtgg aagtagccag actattgaga
     1081 aaggaacacg agtaccaggg ctacatccat cttaagatac ttcccggtgc ttcctacgac
     1141 cacataaaaa gggccatggg attagctgac cggataagcg ttaacttgga atcagcaaca
     1201 gcaaccggtt tttcagagct gaccacaacc aaagattatc aaaaagatgt tctaaggcgt
     1261 atgaaatgga tcaataggtt gaaaaatcgc tacccccagc tagtccctgc tgggcagagc
     1321 acccagttga tggtgggcgc caaccaggaa agcgatgctg atattttgaa acgtgttgat
     1381 ttgttgaaga aaaatttaaa cataaaatta agttacctca gtcctttcac acccttggat
     1441 aacactcctt tatacaacca ttcacaacca gatcctaaac gtgtgccccg cctctatcaa
     1501 gcacagtttc tcattaactc ttatggtttc cccctggaag agatggtgct tgacgattca
     1561 gaacacctta tactggattc tgatcccaaa cagttgtggg cccaactaca tccagagcac
     1621 tnnnnnnnnn nttccagtgg aggtcaatga atcaagtttc aatgatttga taaaagttcc
     1681 aggtattggt cctaaatctg cccgtaaaat tattgctgcc cggagagcag gacatgtttt
     1741 caccacccta gaagatttaa aagggaaggg agtgtggata aagagggctg aaccatttat
     1801 caaattaaac cgggtttatc agtccaccct taacttctaa atcagacccc aaatactcgt
     1861 tataacttct aagttttaac ttattactta taagttataa ttaatggttt taaaaatcct
     1921 ggtcctggaa gagtctccaa atatcaggat atttatcagc aactgcagcc tctattaaaa
     1981 ctgaagcttc cttactaatg ctaaactctg gtttagtttt ctttaaaaat ctaagaactg
     2041 cagctgaacg tgccgaccat agagatatcc gggggttctt atgcacttct attaaaacgg
     2101 cttggatggt gggatcatgg tccttttttg ccttgacatt tggtgttttt gactccttta
     2161 cagatttttt taccccaggt tttcctctag tctctgttgt ctctggttca tcccttttta
     2221 taagggcgtc taatccttta ccaagtgctc ctccagattt tttgggactc atttgttatc
     2281 ctccagttgg atgaactccc tggccagttt tttataggcc tccgtgcccg tgctctcctc
     2341 atcgtagatg acacagggca taccatggct aggagcttca gctaatgtga tgttacgagg
     2401 aattacagtc ttgaacacgt actctctttc accaaagtat tctttgatgt tcctgtaaac
     2461 atcccttcca agacgggttc tgccatcata aagagtgaga agaataccct taataggaga
     2521 cggactcttt aat
//
LOCUS       DAOQVW010000211         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_49966_length_2526_cov_3.829219, whole genome shotgun sequence.
ACCESSION   DAOQVW010000211 DAOQVW010000000
VERSION     DAOQVW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49966_length_2526_cov_3.829219"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKK85_05265"
     CDS             complement(<1..192)
                     /locus_tag="PKK85_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNS25463.1"
                     /translation="MIPVLEPFLGEDELNNVVEAVKSSWISSQGKFIGDFEEGFSNYH
                     RVKHGVSTSNGTAALHLALK"
     gene            complement(429..1064)
                     /locus_tag="PKK85_05270"
     CDS             complement(429..1064)
                     /locus_tag="PKK85_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS25464.1"
                     /translation="MKVKEIMDKEFIEVSPGDSIVDASLKMEEHRKFTTPVVDSQGKL
                     VGWITSLDVTRGLREGQKNIGDIMHSKEDVVHVHQDDPARLAVLNASQHKVVSIPVLD
                     DDDVVKGVVRTFDIVKTLSQLYEVKVYKIFEAMTRELKGVSWEELMEASAIVTRRRTG
                     KRVTPEDYERRIKNSTFGEAIWATGGLEKFFVGLIAIGELVIARKVARARR"
     gene            complement(1054..1821)
                     /locus_tag="PKK85_05275"
     CDS             complement(1054..1821)
                     /locus_tag="PKK85_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25465.1"
                     /translation="MEIIFKKKKEALKNQVALKSLDLDEKPEDFHPEIHAGLLEDKFI
                     TISPDCVRCNLCTEECPVDAIQEPTPNRPARILDNCVKCEICAQSCPVGAVHVMESVS
                     QVDDDVEFRIKDIKTPHRKLRMKEIMVDPAKCKSHGLCTQFCPTGAITVKEGEVAVID
                     KEACVGCGACASVCPEQAITLERDLGPVLKTRELLIDQDKCVQCQVCEENCPVDAIKA
                     GDDLVTLDEDKCILCEVCSTKCPVGALKLKARLPDES"
     gene            complement(1945..>2526)
                     /locus_tag="PKK85_05280"
     CDS             complement(1945..>2526)
                     /locus_tag="PKK85_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNS25466.1"
                     /translation="GVNDELLVKELDYAYAANCNLLHLSSFVGDSIKAQESVLERIPD
                     DVTVSMDPGMLYAHQGLKKLGKLLNCTDILLINQQELDVLLTGQDFEDQVETLFDYDL
                     KILAVKKGSEGCFVSDGEEHHQIYAFEVPCLDTTGAGDAFNSGFLYGILQGYALKDAG
                     VLGNYVASCSIQEYGACRGLPYQEDVPHLREPE"
BASE COUNT          655 a          641 c          489 g          741 t
ORIGIN      
        1 tttaagggcc aggtgtagtg ctgctgttcc attggaagtg gaaactccat gtttaaccct
       61 gtgataattg gaaaagccct cctcgaagtc tcctatgaat ttaccttggg agctaatcca
      121 actgctctta acagcttcaa ccacattatt taattcatct tctcctaaaa atggttcaag
      181 gactggtatc atacagattt ttcttcaaaa agccaacatc agtggatgga taattctttc
      241 aactaccttc ataatataat ggtatcattt tttattgata ttttattcga ttttattttt
      301 ctctctaaga ttatctaaaa cagggtcaaa atacatttaa aagttttgaa gactagttgc
      361 tggtgcatat ccagtcagat ggtaatggga cttgttaata aaaaataaat tataggggat
      421 gtggataatc atcgtcgtgc cctggccacc ttacgggcga ttaccagttc tccaatggct
      481 atgaggccta caaagaattt ttcaagccca ccagtggccc aaatagcttc accaaaggtt
      541 gaatttttaa tccttcgctc ataatcctcg ggggtgactc tcttcccggt cctacgccgg
      601 gttactatgg ctgatgcttc cattagttct tcccatgaga ctcccttcaa ttctctggtc
      661 atggcctcga agatcttgta taccttcact tcgtagagtt gtgaaagggt tttaacaatg
      721 tcaaaggtac gcaccacacc cttcactacg tcgtcatcat ccagcacggg aatactgacc
      781 accttatgct gagatgcgtt aagcactgcc agacgcgccg gatcatcctg gtggacgtgt
      841 acaacatctt ccttactgtg catgatgtcc ccaatgtttt tctgaccttc cctaaggccc
      901 ctggtcacat ccagggatgt gatccacccc accagtttac cctgactgtc aaccactggt
      961 gtggtgaact tgcgatgttc ctccatttta agggaggcat ctacgatgct gtcacctggg
     1021 ctgacttcaa taaactcctt atccattatt tccttaactt tcatctggca acctcgcttt
     1081 aagttttaaa gcccccactg ggcatttggt tgaacaaact tcacataata tgcatttatc
     1141 ttcatctaaa gtcaccaggt catccccagc ttttattgca tccacgggac agttttcttc
     1201 acaaacctgg cactgcacac atttatcctg atcaatgaga agttccctag ttttcagcac
     1261 aggacccaga tctcgctcca gggtgatggc ctgttctgga cagacactgg cacaggcacc
     1321 acaacccaca caagcctctt tgtcaatgac ggctacttcc ccctccttca cggtgatggc
     1381 cccggttgga caaaactggg tgcacaggcc atgtgatttg catttggcag gatccaccat
     1441 gatctctttc atacgcagct ttcggtgggg ggttttaatg tccttaatcc ggaattcaac
     1501 atcatcatcc acctgggaga cgctttccat gacatgcacg gcacccacag ggcaactctg
     1561 ggcacaaatt tcacacttaa cacagttatc aagaatacgg gccggccggt taggggtggg
     1621 ttcctgtatg gcatccactg gacattcttc tgtgcagaga ttacaccgca cacaatcagg
     1681 agaaatggta atgaatttat cttccagtaa accagcatgg atttctgggt ggaaatcttc
     1741 aggtttttca tccagatcca gtgatttaag agccacctga tttttaaggg cctctttttt
     1801 ctttttgaat attatttcca tgaacaatcc cctatttatt gtgaaaaccc aatgaacatt
     1861 attatattgg atactatcat ttttacgcat cccatcatat taatacccca ctcaccctcc
     1921 atcaccccat ttattagggg gcatctattc tggctccctc aagtggggaa catcctcctg
     1981 gtagggcagt cccctgcagg caccatactc ctgtatactg caggatgcaa cgtagttacc
     2041 taaaactcca gcatccttta gagcataacc ctggagtatg ccgtacagaa atcccgagtt
     2101 gaaggcatca ccagcgccgg tggtatccag gcagggcacc tcaaatgcgt agatctggtg
     2161 gtgctcttcc ccatcagata cgaagcatcc ctcactcccc ttcttcacag ccagaatctt
     2221 aagatcataa tcaaaaaggg tttccacctg gtcttcaaag tcctggcctg ttaagagtac
     2281 atccagttcc tgctgattta ttaacagaat atcagtacag tttaaaagtt ttccaagttt
     2341 tttcaatcct tgatgggcat ataacattcc agggtccatg ctaacggtaa catcatctgg
     2401 aattcgttct agaacagact cctgggcctt aattgagtct ccaacaaatg agcttaggtg
     2461 taagaggtta cagttagctg cataggcata gtccagttcc ttaactaaga gctcatcgtt
     2521 aacccc
//
LOCUS       DAOQVW010000212         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_50450_length_2510_cov_2.834623, whole genome shotgun sequence.
ACCESSION   DAOQVW010000212 DAOQVW010000000
VERSION     DAOQVW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50450_length_2510_cov_2.834623"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKK85_05285"
     CDS             complement(<1..496)
                     /locus_tag="PKK85_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate/pantothenate synthetase"
                     /protein_id="HNS25467.1"
                     /translation="MEEIPQSHPRYQSLVLRKKISEAHKAGILAESGMIAHGRGEAFD
                     YLLGEKTTPPARKAIKAAAAQILLAKNPVISVNGNTVALSAREVVELSHKSGAKVEIN
                     LFYRTPERVDKIEKILCEEGADEVLGTNNEEYLHLNGLEGPRSHVSARGIYSADVVLV
                     PLEDG"
     gene            complement(551..1432)
                     /locus_tag="PKK85_05290"
     CDS             complement(551..1432)
                     /locus_tag="PKK85_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2101 family protein"
                     /protein_id="HNS25468.1"
                     /translation="MNIFSIIGDFIIKLFSLIGSIILGIPKIPEKLRGMDTKDIKEKI
                     SQEDLKENFNKFKESTKTTATTITGKGEKSYSKNVDSASVEKRDDDVILISVPFSSEE
                     KERTIFILQIVSAGFLFLTMIYIFNFISLPLLLILGAVMVVYLVYILFKKVKVMYGPD
                     FPAYRDFFLMYILVGIVLVLVGTNSNFVMAFSFEFFPSISLLIFALIAVAVVFLIFRI
                     RYSRDFTFGRVVEAGEKTAYVKVDYDIRSNVKPDLYIVDNLYGAKTGDDVKLKVDGNI
                     ISGGNKPVSILAVMKKI"
     gene            complement(1451..1885)
                     /locus_tag="PKK85_05295"
     CDS             complement(1451..1885)
                     /locus_tag="PKK85_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25469.1"
                     /translation="MDRRGQASAEYLLLIVVILLVMGWLLVNVYQPSIGATMDVSIAS
                     DAKNAVTNIANAANLVYANGPGAKRTLSVYIPASGTFTSNNASKVIQFNSASIANSAH
                     SVNASTSSNMTIENPNVTKGWYNNIVVEWKLNTNYISVTIDK"
     gene            complement(1959..>2510)
                     /locus_tag="PKK85_05300"
     CDS             complement(1959..>2510)
                     /locus_tag="PKK85_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HNS25470.1"
                     /translation="FLISKLPKYVKAGLIAVAVFYSFYTYFQVTLMGIITLLLTLIAI
                     GMVRKLLTSVSREALQDDYPVEGLEEGMILAYKLYQTNGEVEWDEKGFFTKIKETFAK
                     KDLTLISRPPGKLVISSLAAGLTKDDIKLLKKLRKEGKIKEEVRIKRGVPFAPSILIG
                     LLLSLFIGDLAFIIQQLLFNVLY"
BASE COUNT          724 a          543 c          495 g          748 t
ORIGIN      
        1 caccatcctc cagggggact aagaccacat cggcactgta gatccccctg gcactcacat
       61 gtgagcgggg accctccagt ccattcaaat ggagatactc ctcattgtta gtccctaaaa
      121 cttcatcagc accctcttca cagagaattt tctcaatttt atccaccctt tcgggtgtgc
      181 ggtagaaaag attgatctcc acctttgccc ctgacttatg ggataactca accacttccc
      241 tggctgataa agctacggtg ttcccattaa ccgatataac cgggtttttg gccagtagga
      301 tctgggcagc agctgccttt atagccttcc tggcaggggg ggttgttttc tctcccagta
      361 aataatcaaa tgcctcgccc ctgccatggg cgatcatccc cgattctgcc agaatacctg
      421 ctttatgggc ttctgatatc ttttttcgta aaaccaggga ttggtaacgg ggatggcttt
      481 ggggtatttc ctccaattta agtaaactcc tacaaaaaat ttttataaaa aaaattagat
      541 catcaaggta ttagatcttt ttcataaccg ctaggatact gactggtttg ttgcctccgc
      601 tgatgatatt cccatccacc ttcagcttaa catcgtcacc ggttttggct ccgtacaagt
      661 tgtctacgat gtaaaggtca ggtttcacat tggatcggat gtcgtaatcc acttttacat
      721 aggcagtttt ctcccccgcc tctactactc tgccaaatgt aaaatcacgc gaatatctaa
      781 tacggaaaat taaaaatacc accgccacag ctataagggc aaatatcagt aacgatattg
      841 atgggaagaa ttcgaaggaa aatgccataa cgaagttgga gttggttcca accaggacca
      901 gtactattcc taccagtata tacatgagga agaagtcacg ataagcaggg aaatcaggac
      961 cgtacattac cttcaccttc ttgaacaaga tgtaaaccaa ataaactacc atcactgcac
     1021 ccagaataag aagtagagga agggatatga aattgaagat gtatatcatg gtcaggaata
     1081 ggaaaccagc tgagacaatt tgaagtatga atatggttct ctctttttct tcagaactaa
     1141 atggcacaga aataaggatc acatcatcgt cgcgtttttc aactgaagca gaatccacgt
     1201 ttttagaata acttttttcc ccttttccag tgatggtggt ggccgtagtt ttggtggatt
     1261 ccttaaactt gttgaagttt tctttaaggt cttcctggga gattttctcc ttaatatcct
     1321 tggtatccat gcctctaagc ttttcgggta tctttggtat gcctaaaata atggatccaa
     1381 tcagtgagaa taatttaatt ataaaatcgc ctatgatgga aaatatgttc atgaaaatcc
     1441 tcaatgatga ttatttatct atagttacgc taatgtaatt ggtgttgagt ttccattcca
     1501 caacaatatt gttataccat cccttggtta cattgggatt ctcaatggtc atgttagatg
     1561 atgttgaggc atttactgag tgtgcagagt tagctatact ggctgaatta aattgaatta
     1621 ccttcgacgc attattggaa gtaaaagtcc cactggcagg tatatataca cttaaagttc
     1681 tttttgctcc aggaccatta gcatagacta gatttgcggc atttgcaata ttagtcactg
     1741 cattcttggc atcagatgct atagaaacgt ccatggttgc cccgatagat ggttgatata
     1801 cgttgacaag taaccaaccc ataaccagta aaataacgac aataagtaaa agatactctg
     1861 ccgatgcctg tcccctacga tccatggtac tcacatcatc aataatttgt cattaatatc
     1921 cttaagcaat attatgatca aagaactata tatacatatt agtataaaac attgaagagc
     1981 agttgctgga tgataaaggc cagatctcca ataaagagtg ataataaaag gccaatgaga
     2041 attgacggtg cgaaaggaac tccccttttt attctcactt cttctttgat ttttccttcc
     2101 tttcgcagtt ttttaaggag ttttatatca tccttggtaa gcccggctgc cagggaagag
     2161 atcactaatt ttccaggagg gcgactaatg agggtgagat ccttttttgc aaaggtctct
     2221 tttattttgg tgaagaagcc tttctcatcc cactccactt cgccatttgt ctggtacagt
     2281 ttataagcca agatcattcc ctcttcaagt ccttcaacgg ggtagtcatc ctgcagggct
     2341 tcccggctga cactggtcag taacttcctt accatgccga tggcaatcag ggtcagcaag
     2401 agggtgatga ttcccatgag tgtaacctgg aaataagtgt aaaaagagta aaacaccgcg
     2461 acagcgatta accctgcctt cacatacttc ggaagcttcg aaatcaggaa
//
LOCUS       DAOQVW010000213         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_50593_length_2505_cov_3.456327, whole genome shotgun sequence.
ACCESSION   DAOQVW010000213 DAOQVW010000000
VERSION     DAOQVW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50593_length_2505_cov_3.456327"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /gene="cobI"
                     /locus_tag="PKK85_05305"
     CDS             <1..590
                     /gene="cobI"
                     /locus_tag="PKK85_05305"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HNS25471.1"
                     /translation="SAESRPSLALSIVKFILESRDDEYEVLDPVFPMIEDEEALHIYW
                     EEAARQVKERLQQGLDVAFITLGDPTVYSTFSYLEKKIRSTGFEVETIPGITSFTGCA
                     ASAHITLGEKDEIIVIVPKVDERLTPILEHADTAVIMKTSRHSKELQDIVGKDPRRKE
                     IISVQNCGMSDEKIVKGFSRDKKYLSTTIIKFKDQ"
     gene            603..1601
                     /locus_tag="PKK85_05310"
     CDS             603..1601
                     /locus_tag="PKK85_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS25472.1"
                     /translation="MEIQPQYLNLKSFRLESGEVLQDLKIEYATFGRKVTDKAGNLTN
                     GVLYLHGWSGSYSSVGNLDAVIGPGKAVDTNRFFIISPTALGSSGSASPCTTELGPAF
                     PFYTVRDMVNFHMKLLESLGIKHLKGVIGTSMGGFQALQWATDYPDFMDFLIPIATGL
                     KSGRQMNGAYGLMSRIIQEDPAYQGGNYDDNPIGALERGSTIGFLWSMTPEYFEQEFE
                     TDEDFWEAMDERNQEVAALDANDIIWRNQAMLNFNMEVDISDGNSSRIRAKTLVIGIE
                     DDQIFPPSISTIPLAESIEDAEIFLYPSIFGHYGCVRDLEQAADAILSFLESNKMY"
     gene            complement(1611..2396)
                     /locus_tag="PKK85_05315"
     CDS             complement(1611..2396)
                     /locus_tag="PKK85_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNS25473.1"
                     /translation="MVRGTDQQLMMGSKKTRDLAKIYSSPQGARVVQSPVKKEILSLL
                     KNKDMSGSEIVSATGKSKSTVSVHLQDLMKANIISWKPHPQDRRKKIFYLHSRHLGDI
                     SPQREIESDMDLYLDQHVVQSNDPFKFFRFMFRTIRMALLDEGVNIDPILHRAGFKVG
                     EAFYHKLQASTLDQLVKNLSDFWAENHLGRLEVNSLDPPIILAYDCFECEDLPLLGRP
                     ACSFDSGILRAIFSAHMGENVSVEETKCFAQGDECCQFHVNPG"
BASE COUNT          700 a          544 c          586 g          675 t
ORIGIN      
        1 gttcggctga atcaaggcct agtttagcat tatcaatagt aaaatttata cttgaatctc
       61 gggacgacga atatgaagta ctggatccag tttttcccat gatagaggat gaagaagccc
      121 tgcatatcta ctgggaagaa gccgccaggc aggttaaaga acgcctacaa cagggcctgg
      181 atgtggcctt cattacactg ggagatccca ctgtctacag tacattctct tacctggaga
      241 aaaagatccg gagcacaggc tttgaggtgg aaaccattcc tggaataaca tccttcactg
      301 gatgcgcagc atcggcacac attaccctgg gggagaaaga cgagatcata gtcatcgtgc
      361 ccaaggtgga tgaaaggtta actcccattc tggaacacgc tgacactgca gtgattatga
      421 agaccagccg ccacagtaaa gaactgcagg acattgtagg gaaggatccc cggagaaagg
      481 agattatttc tgtgcaaaac tgtggcatgt ctgatgaaaa gatagttaaa ggtttttccc
      541 gggataaaaa atatctctcc accaccataa tcaagttcaa ggaccaatga aggggctaag
      601 ttatggaaat ccagccacaa tatcttaatc tgaagtcttt taggctcgaa tccggagaag
      661 tactgcagga tctgaagata gaatacgcca cattcggacg taaagtcaca gataaggcag
      721 gaaatcttac caacggcgtc ttgtatcttc atggttggtc gggcagttac agttcggtgg
      781 ggaatctaga cgcggtgatt ggcccgggga aagcagtgga taccaaccgc tttttcataa
      841 tatcccccac tgccctgggt tcatcaggtt cagccagccc ctgtaccaca gaattaggtc
      901 ctgcattccc tttttacacg gttagggata tggttaattt ccacatgaag ctcctggagt
      961 ctctgggtat aaaacatctt aagggagtga tagggacttc catgggtggt tttcaagccc
     1021 tgcagtgggc gactgattac ccggatttta tggacttcct gatccccata gccactggcc
     1081 tgaaatcagg aaggcagatg aacggtgcct atgggctaat gagcaggatc atccaggaag
     1141 atccggctta tcagggggga aattatgatg ataacccgat tggtgcatta gaacgcggat
     1201 ctaccattgg atttctctgg tccatgactc cagaatactt tgaacaggag tttgaaactg
     1261 atgaagattt ctgggaggcc atggatgagc gcaatcagga ggtggctgca ctcgatgcca
     1321 acgacatcat ctggcgtaat caggccatgc tgaacttcaa catggaagtt gatatttcag
     1381 atggaaactc atcaagaata agggcgaaaa ccctggtaat tggtattgaa gatgatcaga
     1441 tttttcctcc cagtatcagt acaatcccac tggctgagtc cattgaagat gcggagatct
     1501 ttctttatcc ctctatattc ggccactatg gatgtgtgag ggatttggaa caggcagcag
     1561 atgccatact ctcgttttta gagagtaata aaatgtatta ggtactcctt tcatccagga
     1621 ttaacgtgaa attggcagca ctcatcaccc tgggcaaaac atttagtctc ttccacagag
     1681 acattttccc ccatgtgagc tgaaaaaatc gctcttaata tgccgctatc aaaggaacat
     1741 gcaggtcttc ctaggagtgg cagatcttca cattcaaaac aatcgtaggc cagtattata
     1801 ggaggatcta aactgttgac ttctaaccgg cctaaatggt tttctgccca gaaatcagac
     1861 aggtttttaa ccagctgatc caatgtggat gcctgtagtt tatggtaaaa tgcttcacct
     1921 accttaaatc ctgccctgtg aagaatgggg tcgatgttta ccccttcatc taggagagcc
     1981 atccttatgg tgcggaacat gaagcgaaag aatttgaagg ggtcattgga ctgcaccacg
     2041 tgctgatcaa ggtataggtc catgtcagat tcaatctccc tttgaggtga aatgtctccc
     2101 agatgtctgg aatgtaaata aaaaattttt ttccggcgat cctggggatg gggcttccaa
     2161 ctgatgatat ttgccttcat caaatcctgg agatgaacag atactgttga tttggattta
     2221 ccagtagcgg aaacaatttc cgaaccactc atatccttgt ttttaagtaa agataatatt
     2281 tccttcttca ccggactttg aaccaccctt gcaccctggg gtgatgaata tatcttggcc
     2341 aagtccctag ttttttttga tcccatcatt agttgttgat ctgtaccccg caccatttaa
     2401 tggttcgtac caaccgatac gttcgtcact atccgaacat ttatataggg tgggtcccta
     2461 ccatcatata acgtttgtat cttaacgaac ttattcttgg tgagg
//
LOCUS       DAOQVW010000214         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_51042_length_2492_cov_2.446861, whole genome shotgun sequence.
ACCESSION   DAOQVW010000214 DAOQVW010000000
VERSION     DAOQVW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51042_length_2492_cov_2.446861"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PKK85_05320"
     CDS             complement(<1..688)
                     /locus_tag="PKK85_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNS25474.1"
                     /translation="MIWNEEAECMAPGEKTKLQLEKLQEVVERAYNNVPYYQKRFNEA
                     GIGPEDIETLSDLEKIPFTTKADLRDAYPFGMFAVPPDDIVEVHTSSGTTGKPTVSGY
                     TREDIRIWSEVMARALTMAGADKKDVIQNCYGYGLFTGGLGIHYGTQNVGATVIPISA
                     GNTQRQVEILKDFQSSIITCTPSYALYLAEVLQKEGITGEDLNLKAGVFGAEMWTEEV
                     REEIERRLHLD"
     gene            complement(893..1492)
                     /locus_tag="PKK85_05325"
     CDS             complement(893..1492)
                     /locus_tag="PKK85_05325"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNS25475.1"
                     /translation="MTKKMYELPALPYDYGALAPHIGEEQLKIHHQKHHQAYVDGANA
                     LLEKFDSRPLEFDIKAVAKELSFHVGGFALHKLFWENMGPAAECGGEPTGTIAKYIEE
                     DFGDFQRFKQEFSQAAIGTEGSGWAVLTRCFLTDRLFIMQVEKHNINVIPKFPVLMVL
                     DVWEHAYYLDYKNVRPDYVAAFWNIVNWDVVNQRLEDTL"
     gene            complement(1517..2197)
                     /locus_tag="PKK85_05330"
     CDS             complement(1517..2197)
                     /locus_tag="PKK85_05330"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNS25476.1"
                     /translation="MSEKIYEVRKVKDAKGMPLIGDEFPKMEVHTTNGAMKLPNDMKG
                     KWFILFSHPGDFTPVCTTEFVAFQNRSKEFQDLNVELIGLSVDQVHSHIKWIDWIKDE
                     LGVEITFPIIADTGDVANTLGIIHPSKGSNTVRAVFIVDPRGIMRAMLYYPQELGRNI
                     DEVLRMIRGFQVNEEKGVAIPANWPENELLGEGVIIPPAADVETARKRMAEHKCYDWW
                     FCYIPEEK"
BASE COUNT          556 a          653 c          599 g          684 t
ORIGIN      
        1 catccaagtg caggcgccgc tcaatctcct ccctcacctc ctcggtccac atctccgccc
       61 cgaatacacc ggccttcaag ttgagatcct cccctgttat accctccttc tgcaggacct
      121 cggccaggta cagggcatag gagggggtgc aggtaatgat gctactctgg aagtccttca
      181 ggatctctac ttggcgctgg gtgttgccag ctgagatggg aataacagtg gcccccacat
      241 tctgggtgcc gtaatgtatg cccaacccac cggtgaagag tccgtatcca taacagttct
      301 gtataacatc ctttttatca gcaccagcca tggtgagggc acgggccatt acttcactcc
      361 agatccggat atcctccctg gtgtagccgg ataccgtggg cttgccagtg gtcccggagg
      421 aggtgtgcac ctctacgatg tcatcgggtg gtactgcgaa catgccgaag gggtaggcat
      481 cccggaggtc ggccttggtg gtgaagggta tcttctccag gtcggatagt gtctctatat
      541 cttcaggccc tattcccgct tcattgaagc gtttctggta gtagggtacg ttgttgtagg
      601 cccgttctac cacttcctgc agtttctcca gttgcagttt agtcttctcc cctggtgcca
      661 tgcactcggc ttcttcattc catatcatca gcataacctc ataataattg taaaaatcat
      721 tgcacactca tctaaatata agctgccggt tactattctt ttccttgtag aataaaaaat
      781 gatagggtgg acccctttcc tgaaactagt tggccaaaaa aaataataaa aaaggggggg
      841 ttctatcccc tttcctctcc taccaagacc ctcagatcta aggggtgggg atctaaaggg
      901 tgtcttccag gcgctggttc accacatccc agtttacgat gttccagaag gcagccacgt
      961 agtctgggcg tacattcttg taatccaggt agtatgcgtg ttcccacacg tcaagtacca
     1021 tgagcaccgg gaatttgggg atgacgttga tgttgtgctt ctccacctgc atgatgaaca
     1081 gtcgatcagt taaaaaacag cgagttaaca ctgcccaccc tgagccttcg gtgccgatgg
     1141 ctgcctggga gaactcctgt ttaaatcgtt ggaaatctcc aaagtcttct tcgatgtatt
     1201 tggctatggt gccggtgggt tcccctccgc attcagcagc agggcccatg ttctcccaga
     1261 atagtttgtg caatgcgaag ccacccacat ggaaggacag ttccttggcc acggccttga
     1321 tatcgaattc aagaggacgg ctgtcaaatt tctctaatag agcattcgct ccatccacgt
     1381 aggcttggtg gtgtttctgg tggtgtatct tcagttgttc ctcaccgatg tgaggtgcca
     1441 gggccccgta gtcgtatggg agggctggta attcgtacat cttcttggtc attgctaaca
     1501 tctccctggg gacagattat ttttcttcgg gtatgtagca gaaccaccaa tcgtagcatt
     1561 tgtgctcggc catcctttta cgggcagttt ccacatcagc agctggagga atgatcactc
     1621 cttcaccgag gagttcgttt tcaggccagt tagctggtat agctacgcct ttttcctcgt
     1681 tgacctggaa gcccctgatc atcctgagaa cttcatctat gttacgcccc agctcctggg
     1741 ggtagtagag cattgccctc ataatacccc gggggtccac gatgaaaact gcacggacgg
     1801 tgttggagcc cttggagggg tgtatgatgc ccagcgtgtt agccacgtca ccggtgtctg
     1861 cgatgatggg gaacgttatc tccacgccca gttcatcctt gatccagtcg atccatttga
     1921 tgtggctgtg cacctggtcc acactgagtc cgatcagttc cacgttaaga tcctggaact
     1981 ccttgctacg gttctggaag gccacaaatt cagtggtgca cactggtgtg aagtcaccgg
     2041 ggtggctgaa caggatgaac cacttaccct tcatatcatt aggtagcttc atcgccccat
     2101 tagtggtatg aacttccatc ttagggaact cgtcacctat aagtggcatc cccttggcgt
     2161 ccttaacctt gcgtacttcg taaatttttt cactcatatt taatcaccta ctataactag
     2221 atatcttgga tgttattcat cattaacatt tcgatttcta gatctgaaac tggaaagcta
     2281 gccacaaatg tacacttcca ataccctacc tggatgatcc taaaaaataa tctattattc
     2341 tatgcccctt taaaaaaaaa ggtggaaagg agttgggcta ctcctctgcc gggggatgga
     2401 aaaccctttg ccccagttcc tgatccagca tgtagaggcc gcttctagac tggctagcca
     2461 ttttaacctt gtgcaggacg ccgctggatg at
//
LOCUS       DAOQVW010000215         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_51370_length_2482_cov_2.717759, whole genome shotgun sequence.
ACCESSION   DAOQVW010000215 DAOQVW010000000
VERSION     DAOQVW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51370_length_2482_cov_2.717759"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="thiD"
                     /locus_tag="PKK85_05335"
     CDS             <1..648
                     /gene="thiD"
                     /locus_tag="PKK85_05335"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNS25477.1"
                     /translation="AQNVKRVAGVEEVSLDFVEKQLDLVMEEYPIHYAKTGMLHSPEM
                     VKLVARKLEEYQLDVVVDPVMVAGSGGMLSQKGMSRALKKYLLPQAVLVTPNLQEAQE
                     LSGIRIMDEEDAIQAAKEIGRLVPVVVTGGHLHGHDIFYNGRVQIIKGELIKSSHTHG
                     SGCTYSAAVTAYLSRGLALEEALPRAAKLVKEAIRKGCLGTLNQFFLADHRGTSK"
     gene            726..1610
                     /locus_tag="PKK85_05340"
     CDS             726..1610
                     /locus_tag="PKK85_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNS25478.1"
                     /translation="MVSNEEIKKMLENRRRGIKPEAKKEQPTPRRPATSRRPVKTRTL
                     SCGSCGAENPPDSKFCINCGAKMQDQEVADEKKPAETKDALPLSGMEGTDYQTCPNCK
                     HQNKPQAKFCVVCGHKLGPVEEVKSTASLLDRVPDEEAAKEKPSEAKFPSVTELMDEE
                     ADQEKPPKRSLSPKQAPTLDTSTGPVETEPYPAAEEEQVSVEETSQDEGAGPELQGAS
                     SPDKMVLEEKAAEAPMEEPDLPRGKTPEGVAEDAALTGEIQVDPVDKIKKAKELLDIG
                     AISQEEFEEIKKKYLSLI"
     gene            complement(1710..2141)
                     /locus_tag="PKK85_05345"
     CDS             complement(1710..2141)
                     /locus_tag="PKK85_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNS25479.1"
                     /translation="MKLKQISVFLENRKGRLWKAMNVLATADINIRALSIADTSEFGI
                     LRMIVPDPEKAQRVLEKNHFVVKVNQVIAVEVPDEPGGLDHILGVLTQADINVEYLYA
                     FVEKNEEKAIVVIRTEDVDAGIQALEAAQVTILSADEVYKI"
     gene            complement(2170..>2482)
                     /locus_tag="PKK85_05350"
     CDS             complement(2170..>2482)
                     /locus_tag="PKK85_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNS25480.1"
                     /translation="KIRGVIVFPSQIERALLKIPGLEPHYQIIVTRPQHLDELEVHVE
                     ASPALFSDEVKHVEKAKEMIQKRIHDEIGLRVNVNLVEPESIPRSEGKAVRVVDKRKF
                     D"
BASE COUNT          693 a          644 c          620 g          525 t
ORIGIN      
        1 gcccagaatg tgaagcgggt ggcaggagtg gaggaggtgt ccctggattt tgtggaaaaa
       61 cagttggacc tggtaatgga agagtacccc atccattacg ccaagaccgg gatgctccac
      121 tcaccagaga tggtgaagtt ggtggcccgg aagttggagg aataccagtt ggatgtggtg
      181 gtggacccgg tgatggtggc tggatccggt ggaatgctct cccagaaggg gatgagccgg
      241 gcccttaaaa agtatctgct cccccaggca gtgctggtca cccccaacct ccaggaagcc
      301 caggaattat cagggatccg aatcatggat gaggaggacg ccatccaagc tgcaaaggag
      361 atcggccgac tggtgccagt ggtggttaca ggtggacacc tccatggcca tgacatcttc
      421 tacaatggca gagttcagat tattaaagga gaacttataa aaagcagcca cacccatggc
      481 agcggatgca cctactccgc ggcggtaact gcctacctct cccggggatt ggcactggag
      541 gaggccctcc ccagggctgc caaactagtg aaggaggcca tcaggaaggg ctgtttaggt
      601 accctgaacc agtttttttt agcagatcac agggggacct caaaatagat aaacaagtac
      661 cccacaaaag tatgcattaa atcatcatag agaatcaatt tttattcaaa aaggagagtg
      721 aacttatggt ttccaatgaa gagataaaaa aaatgctcga aaaccgcaga agaggaataa
      781 agcccgaggc taaaaaggaa caacctacac cgcgtcgtcc cgccacatcc cgaagaccag
      841 ttaaaaccag aacccttagc tgcggatcct gtggagcgga gaacccacct gactccaagt
      901 tctgcataaa ttgcggggca aagatgcagg accaggaggt ggcagatgag aagaagccag
      961 cagaaaccaa ggatgcctta cccctctccg ggatggaggg tacggactac cagacctgcc
     1021 ccaactgcaa acaccagaac aagccccagg ccaagttctg tgtggtgtgc ggccataaac
     1081 taggaccagt agaagaagtc aaatccactg cttcgctcct ggatcgggtg ccagatgaag
     1141 aagcagccaa ggaaaagcca tcagaagcta agttcccatc ggtgacagaa ctcatggatg
     1201 aagaagcaga ccaggaaaaa ccccccaaaa gaagcctctc acccaaacag gcccccaccc
     1261 tagacaccag caccgggccg gtggaaactg aaccataccc ggctgcagaa gaagagcagg
     1321 tgtcagttga agaaactagc caggatgagg gggctggtcc tgaactgcag ggtgcatcta
     1381 gccctgataa gatggtcctg gaagaaaaag cagcagaagc gccaatggaa gaaccagatc
     1441 taccccgagg taagacccct gaaggagttg ccgaagatgc agctttaact ggtgaaatcc
     1501 aggttgatcc agttgataag attaaaaaag ccaaagaact tttggatata ggagccatat
     1561 cccaggaaga gtttgaggag ataaaaaaga agtacctctc cctcatataa aaaataataa
     1621 tatgggtaca tggggggtct ttaaaaaata aatccctata taccccctac attttttttt
     1681 ctacgcacac tagtaactta ttactcacct tatatcttgt aaacctcatc tgcagagaga
     1741 atagttacct gtgctgcttc cagggcctgt atacccgcgt ccacatcctc ggtacggatc
     1801 actacaatgg ccttttcctc atttttctct acaaaggcgt agaggtattc tacgttaata
     1861 tcagcctggg tcagaacccc caggatgtga tccagccccc ctggttcatc aggcacctcc
     1921 acggcaatga cctggttgac cttcaccacg aagtgattct tctccagcac cctctgggcc
     1981 ttctccgggt caggcacgat catgcgcagg attccgaact cggaggtgtc ggcaatggac
     2041 aaggcccgga tgttaatatc cgctgtagcc agtacattca tggccttcca cagtcttccc
     2101 ttccggtttt ccaaaaacac tgatatctgt ttgagtttca tccacatccc cttaccaatt
     2161 atcaattttt tagtcaaatt ttcgtttatc aacaactctc actgccttgc cctcgctacg
     2221 gggaatgctc tctggttcca ccaggttaac gttaacccgc agtccaatct cgtcatggat
     2281 acgtttctgg atcatttcct tggccttctc cacgtgcttc acctcatcag agaacagggc
     2341 cggggaggcc tccacatgca cctccagttc atccaggtgc tggggccggg tcacaatgat
     2401 ctggtagtgg ggttccagtc ccgggatctt caggagggcc cgttcgatct gggagggaaa
     2461 cacgataacc cccctgatct ta
//
LOCUS       DAOQVW010000216         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_51686_length_2471_cov_3.034768, whole genome shotgun sequence.
ACCESSION   DAOQVW010000216 DAOQVW010000000
VERSION     DAOQVW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51686_length_2471_cov_3.034768"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /gene="dnaJ"
                     /locus_tag="PKK85_05355"
     CDS             complement(<1..1010)
                     /gene="dnaJ"
                     /locus_tag="PKK85_05355"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNS25481.1"
                     /translation="MADKRDYYEVLGVDREATKKDIKKAYRKLAMEYHPDVSDDPGAA
                     DKFKEISEAYAVLSDDEKRGTYDQFGHAGMDGFSQEDIFNNINFEDIFRGFGFGGSGR
                     GGFETIFDLFGFGGGRRNGPQRGADVVYDMKITLEEAATGIERDIEVPHKQVCPRCHG
                     TRAEPGTDTRTCETCGGSGQVRHVQNTPLGQFASVRPCSTCHGEGTIIDNPCHECQGK
                     GIVKQKNTINIKIPPGVENGSRLRVSGQGDVGVKGGPPGDLYVMIAVKHHRLFQREGA
                     NLYYEKPISFVQATLGDKVEIPTLDGAVDLKIPSGTQTGTSFRIKGEGMPHLRWNGKG
                     NL"
     gene            complement(1158..>2471)
                     /locus_tag="PKK85_05360"
     CDS             complement(1158..>2471)
                     /locus_tag="PKK85_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNS25482.1"
                     /translation="GTLDVTIMEFGGGVFEVRSTSGDTKLGGTDMDNAIMNYLAQEFQ
                     KETGVNLLNDDQAVQRLKEAAEKAKIELSTTLKTDINLPFITATQDGPKHLTHTLSRA
                     KVEELVDPIIKRCAVPMEQAIKDAKMSKSDIDKIILVGGPTRMPIVQKFVEDYIGKPV
                     ERGIDPMECVAVGAAIQGGVLAGEIKDLVLLDVTPLSLGIETLGGVFTKLIERNTTIP
                     TKKSQIFSTAADNQTSVDIHVLQGERPVASGNTTLGRFQLVGIPPAPRGIPQIEVTFD
                     IDANGIMNVSAKDMGTGKEQAITITAPNKLSEDEIEQKVKEAEMHAEEDKKRQEEVQI
                     RNDADSMIYTSEKTLEELGDKVQADQKTRIEELVKELRELVAGDDLEAIKSKTEELTK
                     VVQEVGAAIYQQAQQAQAEQQQGPPGSDTQGPGDEDAVDAEYEKK"
BASE COUNT          542 a          619 c          539 g          771 t
ORIGIN      
        1 taaaggttac cttttccatt ccagcggaga tggggcattc cttctccttt aatacggaat
       61 gaagttccag tctgggttcc ggatgggatt ttcaaatcca cagcaccatc tagggtgggt
      121 atctcaactt tatcgccgag agttgcttgg acaaagctaa tgggcttttc ataataaaga
      181 ttggctcctt cgcgctggaa aagacggtga tgttttactg cgatcatcac ataaagatct
      241 ccagggggcc cgcctttcac acccacatct ccctggccag ataccctgag tcgagatcca
      301 ttctccactc ccggaggtat tttgatgttg atggtgtttt tctgtttcac aattccttta
      361 ccctgacatt catgacaggg attgtcgatg atagttcctt cgccatggca agtgctgcag
      421 ggcctgaccg aggcaaactg tcccaaggga gtattttgaa catggcggac ctgaccacta
      481 cctccacagg tttcacaagt acgggtatct gtaccgggtt cagcccgggt tccatgacag
      541 cgggggcaga cctgtttatg tggaacttca atgtccctct caattcctgt ggctgcttct
      601 tccagggtta ttttcatgtc gtagaccaca tcagcgccac gttgcgggcc attccttcta
      661 cctccaccga aaccgaaaag atcgaatatg gtttcgaagc caccccttcc acttcctccg
      721 aatccgaatc cacggaagat gtcttcgaag ttgatgttgt tgaaaatatc ctcctggctg
      781 aaaccgtcca taccagcgtg tccgaattgg tcataggtgc ctcttttctc atcatcggat
      841 aacactgcgt aggcttcact gatctcttta aatttatcag ctgcccctgg atcatcactt
      901 acatctggat ggtactccat agccaatttc cggtaagcct tctttatatc cttcttagta
      961 gcttcacggt ccacacccag gacctcatag taatcccgct tatctgccat gtataatctt
     1021 ccttaatctt ctgataatat taaaattaaa tagataaaca aatcctcgat aattatttcc
     1081 ttctgaatgg atggtttgta aaggatataa taaaaatttt gggaaaaaga tgtaaaatag
     1141 aggagtggat acaacctcta ttttttctca tattccgcat ctactgcgtc ttcatccccg
     1201 ggaccttgtg tatctgatcc cggtggtcct tgttgttgct cagcttgtgc ttgttgagcc
     1261 tgttgataaa tggcagcccc aacttcctgt accaccttgg tgagttcctc ggtcttgctc
     1321 ttaatggcct ccagatcatc cccggccacc aactcccgaa gttctttaac cagttcttca
     1381 atcctggttt tttggtcggc ctgtacctta tcgcccagtt cttccagggt tttctcggag
     1441 gtgtagatca tgctgtcggc atcgttacgt atttgaactt cttcctgtcg ttttttatct
     1501 tcctcagcat gcatctcagc ttctttgacc ttctgttcaa tttcatcctc ggaaagcttg
     1561 ttaggagcgg tgatggtaat ggcctgttcc ttaccagtac ccatgtcctt ggcggacacg
     1621 ttcatgatac cattggcatc gatatcaaag gtaacctcta tctgtgggat tccacgtggt
     1681 gcaggtggta ttcctaccaa ttggaatctt ccaagggtgg tgttcccact ggcaacggga
     1741 cgttcacctt gtaaaacatg gatgtctaca gatgtttgat tatctgccgc agtactgaat
     1801 atttgactct ttttggtggg tatagtggtg ttacgttcga ttaacttggt gaaaactcca
     1861 cccagggttt caatacctag tgataagggg gtaacatcca gtaaaaccag atctttaatt
     1921 tcgccagcca gcacacctcc ctgaatggcg gcacctacag ccacacattc catgggatcg
     1981 attcctcttt caacaggttt gccaatgtag tcttccacga atttttgaac tatgggcatc
     2041 ctggttggtc ctcctaccag tataatcttg tcaatatcgc ttttagacat ttttgcgtct
     2101 ttaatggcct gttccattgg cactgcgcat cgtttaatga tgggatccac cagttcctct
     2161 actttcgccc ggctcagggt atgagtcaag tgtttaggcc cgtcttgggt ggcagtgatg
     2221 aaggggaggt taatatccgt tttgagggtg gttgacagct caattttggc cttttcagct
     2281 gcttctttta acctctgcac ggcctggtca tcgttaagga ggtttacacc ggtttctttt
     2341 tggaactcct gggccaggta gttcatgatg gcgttgtcca tgtcggtacc acccaatttg
     2401 gtgtctccac tggtggatcg aacctcaaat actcctcctc cgaattccat gatggtgacg
     2461 tctagggtac c
//
LOCUS       DAOQVW010000217         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_51873_length_2466_cov_3.605973, whole genome shotgun sequence.
ACCESSION   DAOQVW010000217 DAOQVW010000000
VERSION     DAOQVW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51873_length_2466_cov_3.605973"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..1203
                     /gene="nadC"
                     /locus_tag="PKK85_05365"
     CDS             367..1203
                     /gene="nadC"
                     /locus_tag="PKK85_05365"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNS25483.1"
                     /translation="MRQDLARLVYEDIGFEDLTTQALIPPSMVIRGRIVAQEEGVVAG
                     LELASTIFREFSIQTSLKKQDGDWVAPWEVLMEVEGEARSILSVERTALNLLMRMSGI
                     ATLTHSIIEMVREVNPTVIIAGTRKTTPGLQFFEKDAIRVGGGDTHRYRLDDAVLIKD
                     NHLALVGSVTEAVERARKHASFTKKIEVEADTLDQALQAAQAGADIVLLDNMSLDQIR
                     TVLSLLKNGGLRDKVLVEASGGINPDNIMAYAKLEVDIISTGYITHSARSLNLNLELD
                     GE"
     gene            1394..1882
                     /locus_tag="PKK85_05370"
     CDS             1394..1882
                     /locus_tag="PKK85_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25484.1"
                     /translation="MDEQEMNEYFQEDAEEFTEEVTDNQKPEEEFTEEVQDESEIEEE
                     DESLPFAKAEVVRLMKQNLDSDKMIRERVKVEMNKFLGEVLRKVCYQLNQYPYTTIEY
                     EMLKESIYPYENIERINEEKKRILKHLDAIKADCDALSMDVKVTLRLNASDDTDEDDI
                     FK"
     gene            complement(1905..2465)
                     /locus_tag="PKK85_05375"
     CDS             complement(1905..2465)
                     /locus_tag="PKK85_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HNS25485.1"
                     /translation="MSNCPICGNLKCVEVNTRTDNIPYFGEIMESTLLCRECGYKHSD
                     TICLEQKEPVHYSLLIGKKELNTRVIKSQSATLSIPELGLKVEPGPKSQGYVSNVEGV
                     LERFEKAVATALAWADDPVAQDNAKDILEALHQVRYEERQTELVVEDPFGHSAILHPE
                     ARKRDLTTDELEKLKTGFTIINSEEE"
BASE COUNT          748 a          506 c          572 g          640 t
ORIGIN      
        1 ctgggattcc accaacgatc tacgggtgat gaacaagggt taatcacagt taaaaaaaat
       61 aggcaatgca aacaaatcca taacctttat cctcggaggt tcaagggtgg ccacttttgg
      121 tgtggaaaaa ggcatccttg tttttaaaaa aggtgtaagt aaggagttaa tatagattaa
      181 ttttagtatt tccaaaaatt cagtagctaa gattctcctt tggtgaaata ataggaatta
      241 tgtttttgga attctataat aattttatgg ggtagatgta aatctttagt aatgataaca
      301 cattactatc ctgattggga atagatttag atgaaagata aacaacattt ttctggtgca
      361 gatatcatga gacaagacct ggcccgtctg gtatatgaag atataggatt cgaggacctc
      421 acaacccagg ccctgatacc cccctctatg gtaatcagag gtcgcatcgt ggcccaggaa
      481 gagggtgtag ttgctggcct ggaactggcc agtaccatct tcagggaatt ttccatccaa
      541 acatccctaa aaaaacagga cggggactgg gtagctccct gggaggtctt aatggaagtg
      601 gaaggtgaag cacgttccat attaagtgtg gagagaacag cccttaacct gctgatgcgc
      661 atgtccggca tagccaccct aacccacagc atcattgaaa tggtccggga ggtaaatccc
      721 actgttatca tagctgggac aagaaaaacc acccctggcc ttcaattttt cgaaaaggat
      781 gccatacggg taggtggagg ggacacccat cgctatcgtc tggatgatgc tgtactcatt
      841 aaggacaacc acttggccct ggtgggcagc gtaactgagg cagttgaaag agcccgtaaa
      901 catgccagct tcaccaagaa gatagaagtg gaagcggaca ccttagatca ggcccttcaa
      961 gcagcccagg ccggtgcaga tatcgttctt ctggacaaca tgtcccttga ccagataagg
     1021 acagttctat cattactaaa aaatgggggg ctgcgggata aggtgctggt agaagcctcg
     1081 gggggtataa atccagataa tataatggcc tatgccaagt tagaggtgga tatcatatcc
     1141 accgggtaca taacccactc ggcaaggtcc ctgaacctta acctggaatt ggatggggaa
     1201 tagtgtgcac aatatatata ctggtaagtt tacaatggat aagctgtgca agctatttta
     1261 aaatttttat agataccttt cattattatc atggaatcct cgtttactat caatattatt
     1321 tgatgaaatt ttcattttaa ccacagtatt taaataaagt gatatctaga ttaagaacat
     1381 ggagattgat aggatggatg aacaggaaat gaatgaatac ttccaagaag atgccgaaga
     1441 attcacagaa gaagttacag ataatcagaa accggaagaa gaattcacag aagaagttca
     1501 agatgaatca gaaatagaag aagaagatga aagcctcccc tttgccaaag cggaagtggt
     1561 acgtctcatg aagcagaacc tggatagtga caagatgatc cgggaacggg tgaaggtgga
     1621 gatgaacaag ttcctggggg aagtcctgag gaaggtctgt taccagctga accagtaccc
     1681 ctacaccacc atagaatatg aaatgctcaa ggagtccatc tacccctacg agaacattga
     1741 aaggatcaat gaggagaaga agaggatctt aaaacacctg gacgcaataa aagccgattg
     1801 tgatgccctc agtatggatg taaaggttac tttaagactc aacgcctcag atgacaccga
     1861 tgaagatgat atttttaaat aaaatatcct tcattttttt tattctactc ctcttctgaa
     1921 ttaatgattg taaaaccagt tttaagtttt tccaactcat cagtggtaag atcccttttc
     1981 ctggcctctg ggtgtaggat ggcgctatgg ccaaagggat cctccaccac cagttccgtc
     2041 tgccgctcct cgtacctcac ctggtgaagg gcttccagta tgtcttttgc attatcctgg
     2101 gcaacaggat catcagccca ggccagggca gtggcaacag ctttctcgaa tcgttccagc
     2161 accccttcca cgttggaaac atagccctgt gattttggtc cgggttccac cttaagtccc
     2221 agttcaggta tgctgagggt ggcggattgg gatttgatta cccgggtgtt cagttctttt
     2281 ttcccaataa gcagtgaata atgcacaggc tctttctgct ccagacagat ggtatcagaa
     2341 tgtttgtacc cacattcacg gcacagtagg gtggactcca ttatctcccc aaagtaggga
     2401 atattatcgg ttctagtgtt aacctctaca catttcagat taccacaaat gggacaattc
     2461 gagatc
//
LOCUS       DAOQVW010000218         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_52684_length_2441_cov_3.461023, whole genome shotgun sequence.
ACCESSION   DAOQVW010000218 DAOQVW010000000
VERSION     DAOQVW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52684_length_2441_cov_3.461023"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PKK85_05380"
     CDS             complement(<1..768)
                     /locus_tag="PKK85_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS25486.1"
                     /translation="MAYLAHISDLHVGSVDFREELLLQVVDELNSSNPDATIVTGDLT
                     ENGYYSEFVEAAEYLDRINTPMLVVPGNHDARHVGDQTFREIIRGRYSTLVKGMYSTL
                     NVKSQDLQVIGLDSSEPDLDYGKIGRSQEKWMVEELHQAHEQGLYRIIALHHHIIPVP
                     RTGRERNVLSDAGDILLSMCRNHANLVLSGHKHIPHVWMVEDTAFATAGTVSSLKLRG
                     KQLSSYNTVDVDEDHIEILLNSADGEVTSLAKYENTCR"
     gene            complement(782..1138)
                     /locus_tag="PKK85_05385"
     CDS             complement(782..1138)
                     /locus_tag="PKK85_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HNS25487.1"
                     /translation="MLPGAGMNPKQMKQMQRAMKQMGMDMKDMRGVEEVVIRLKDKEL
                     VIKNPKVNVMNFMGQNTYQVTGKAKEQLLEKELAIPDDDVDMVAAQTGVTKEEARHAL
                     EETQGDLAEAIMKLSS"
     gene            complement(1138..>2441)
                     /locus_tag="PKK85_05390"
     CDS             complement(1138..>2441)
                     /locus_tag="PKK85_05390"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HNS25488.1"
                     /translation="PMVFALAVAMGCDLFDSAAYILYAQDGRLLMPSGTYKLENLVEM
                     PCTCPVCSNHSPGELRSMDEDERTLLLAQHNLAISFAEIRMIRQAIVEGSLWELVEQR
                     CRAHPYLLDGLRRLGAYQEGLESYDPPYKKSAFFYTGPESLMRPEVHRHWEKIKKLPR
                     KEAVLLLSPVAKPYSQNLTHYLGVVHYRGDCEGVVEGKVPLQLAVVDVPFGVIPLELD
                     QVYPLAQNETPTIMDRDAHHQVKEMVEEYLEGCETVLVEGELARKYQLELDEEATIIN
                     PLPLDTIDLERVRMIADYQFQAGAGRALFDGQVRVVKSRKTGKIRHLYQGEDLIATLR
                     ASDGVLVLAALGAKRLHQYLPYPLNRVVVNEEAEPFAREGKSIFAKFVIDCDIDIHAQ
                     EEVLIVNSEDELLAFGKSILNGREIIDFQTGQAVKTRKGGL"
BASE COUNT          469 a          718 c          623 g          631 t
ORIGIN      
        1 cctgcacgtg ttttcatact tggccaggga ggttacctct ccatcggcgc tgtttaacag
       61 tatctcgata tgatcttcat ctacgtccac cgtgttgtag gaggacagtt gcttgcccct
      121 taacttcagg gaggaaaccg tgcccgccgt ggcaaaggca gtgtcctcca ccatccacac
      181 gtggggtatg tgtttatggc ctgatagaac caggttagca tggttcctgc acatggatag
      241 tagtatgtca ccggcatcgc ttaaaacgtt tctctccctt ccggtgcggg gaaccgggat
      301 gatgtgatgg tggagtgcta tgatccggta caggccctgt tcatgggcct ggtgcaactc
      361 ctccaccatc catttctcct ggctcctacc tatcttacca taatccaggt ccggttcgct
      421 gctatccaat cctataacct gcaaatcctg acttttaaca ttcagggtgc tgtacatacc
      481 cttcaccagg gtgctgtacc gtccccggat gatttcccgg aaggtctggt cccccacgtg
      541 ccgggcgtcg tggttacctg gcaccaccag catgggggtg ttgatcctgt ccaggtactc
      601 cgcggcctct acaaactcgg agtagtagcc attctcggtg aggtccccag tgactatggt
      661 agcgtcgggg ttgctgctgt tcagttcatc caccacctgg aggaggagtt cctcccggaa
      721 atccacggaa cctacgtgca gatcagatat atgggcaagg taggccattt ttcatgtacc
      781 tctaggaact tagcttcatg atggcctcgg ccaggtcgcc ctgggtttct tccagggcat
      841 gacgggcctc ctccttggtg actccggtct gggcagccac catatccaca tcatcatcag
      901 ggatggccag ttccttctct aacagttgct ccttggcttt accagtcacc tggtaggtgt
      961 tctggcccat gaagttcatg acattgactt tggggttttt aataaccagt tccttatcct
     1021 tcaggcgtat caccacttcc tccacgcccc tcatatcctt catgtccatt cccatctgct
     1081 tcatggcccg ttgcatctgc ttcatctgtt tggggttcat ccctgctcct ggtaacatta
     1141 aagtcctcct tttcttgtct taactgcttg accagtttgg aagtcgatga tctcccggcc
     1201 atttaaaatg gacttaccaa aggccaggag ttcatcctca ctgttaacga ttagcacttc
     1261 ttcttgtgca tggatatcta tgtcacaatc tataacaaat ttggcaaata tacttttgcc
     1321 ttcccgggcg aagggttcgg cctcctcatt aacaaccacc cggttaaggg gatagggaag
     1381 gtactggtgt agcctcttgg cccctagggc tgccaagacc agcaccccgt cactggcccg
     1441 cagggtggct attaaatcct ccccctggta gaggtgccgg atcttaccgg ttttcctact
     1501 cttcaccacc cttacctggc catcaaagag ggccctccct gcaccagcct ggaactggta
     1561 gtcagcaatc atcctgaccc gttccaggtc aatggtatcc aatgggaggg ggttgatgat
     1621 ggtggcctct tcgtccaact ccagctggta ctttctggcc agttcaccct ccaccaggac
     1681 tgtttcgcag ccctccaggt actcctccac catctctttc acctggtgat gggcgtcccg
     1741 gtccatgatg gtgggggttt cattctgggc cagggggtag acctggtcca gctccagggg
     1801 tatcaccccg aatggtacat ccaccactgc cagttgtaaa ggcaccttcc cctccactac
     1861 tccttcacag tcaccccggt agtggaccac ccccaggtag tgtgtcaggt tctgggagta
     1921 gggcttggcc accggtgata gtaacaggac cgcctcctta cgggggagtt tcttaatctt
     1981 ctcccagtgc ctgtggacct cgggtcgcat cagagattct ggtccggtgt agaagaaggc
     2041 cgacttcttg tagggtggat catagctttc caatccctcc tggtaggctc ccagcctccg
     2101 caatccatct aaaaggtagg gatgggcccg gcagcgctgc tccaccaact cccagaggct
     2161 gccctccacg atggcctggc ggatcatgcg gatctcagcg aagcttatgg ccaggttatg
     2221 ctgggccagg agtagggtgc gttcatcttc atccatactc ctcaactccc cggggctgtg
     2281 gttgctgcac accgggcagg tgcagggcat ctccaccagg ttctccagtt tataagtccc
     2341 actgggcatg agtaaacggc cgtcctgggc gtagaggatg taggctgcag agtcgaatag
     2401 gtcgcagccc atggccactg ccagggcgaa gaccatgggg t
//
LOCUS       DAOQVW010000219         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_52757_length_2439_cov_3.446309, whole genome shotgun sequence.
ACCESSION   DAOQVW010000219 DAOQVW010000000
VERSION     DAOQVW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52757_length_2439_cov_3.446309"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(371..1603)
                     /locus_tag="PKK85_05395"
     CDS             complement(371..1603)
                     /locus_tag="PKK85_05395"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNS25489.1"
                     /translation="MAIINENYLLLKSSYIFSEIAQRVEKYEKENPEARVIRMGIGDV
                     TLPLPRAVVEGFKEAVEEMGEKDSFMGYGPEQGYHFLVEDIIKHDYQALGIQLSPDEV
                     FISDGAKCDTGNIQEIFGLDNTVAVTDPVYPVYVESNVMAGRTGPMDDDGHYQNLVYL
                     PCTSENNFIPELPSQKVDLIYLCYPNNPTGTALTREQLKTWVDYARDHKAVILFDAAY
                     EAYITKEDIPRSIYEIEGARECAIEFKSYSKSAGFTGTRCAYTVVPKELVAYDKNGNE
                     HDLNSLWNRRQSTKFNGVSYPVQKAAQATYTPQGREEIKENVAYYMANARIIRDGLEE
                     MGLEVYGGINSPYIWVKTPKNMTSWEFFDLLLEEVQVVGTPGVGFGPSGEGYFRITAF
                     NTLENTEEAMERMGRLEF"
     gene            complement(1624..>2439)
                     /locus_tag="PKK85_05400"
     CDS             complement(1624..>2439)
                     /locus_tag="PKK85_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNS25490.1"
                     /translation="SHVLRGKDHMANTEKQGYLYRHLDWEEPHYNHYGRLKMEDMALS
                     TSKAKKGIDEGEYTGWDDPRLGTLRAVRRRGIQAQSIRELMMEIGVKSADTTVSWKKI
                     YGLNRSHLEEVNRYFLATPARQVKILGLPREAKGPVERPLHPDHPERGMRTLNFEDRI
                     YLSSTDIPKKPGQVVRLMDAVNITFQEGEARYHSTSLEEAREHGASIVQWVPMREKKL
                     VEMVMPDGSVQTGYAEADTYQQEEGDVVQFERVGFACLDEKYLGKMTFYYAHK"
BASE COUNT          427 a          679 c          639 g          694 t
ORIGIN      
        1 tttattttat gagggccatt tttggtggtc cttagataat gtttcaaaaa aaaggggggg
       61 atgtgtccct tttttttact tatagtacgg ttttatcagg aaaaatttgt gtagaaaccc
      121 tcaaaaaaaa atgtttcttg ttcggccttg cccaccagtt catggctcga gctttgtttt
      181 ttctgaagga tgcagtttga gtagtaattt tccactgagc acccattcat caaagatagg
      241 aggtggccag tttagggggg aagttaccag cccaatgtat gggggtggtg gtatagccac
      301 cagtctttaa aaaaatgttt tttgaaacca ttaccccatt ttttttggtt gggatcttcc
      361 tcccctagct ttagaattcc aatctcccca ttctctccat ggcctcttca gtgttctcca
      421 gggtgttgaa ggcggtgatc cggaagtaac cctcgccgct gggtccaaac cctacgccgg
      481 gtgttcccac cacctgcacc tcttctagga ggaggtcgaa gaactcccag cttgtcatgt
      541 tcttgggggt tttcacccag atgtagggac tgttaattcc accgtagact tccaggccca
      601 tctcttccag gccgtccctg atgatccggg cgttggccat gtagtaggct acgttctcct
      661 ttatctcctc cctgccctgg ggggtgtagg tggcctgggc tgccttctgc accgggtagg
      721 agaccccgtt gaatttggtg ctctgcctgc ggttccagag gctgttcaag tcgtgttcat
      781 ttccattttt gtcgtaggcc accagttctt taggcaccac ggtgtaggca cagcgggtcc
      841 cagtgaagcc cgcacttttg gagtagctct tgaattcgat ggcacactcc ctggcccctt
      901 cgatctcata gatactccgg ggtatgtcct ccttggtgat gtaggcctcg taggctgcgt
      961 cgaataagat gactgctttg tggtccctgg cgtagtccac ccatgttttg agttgctccc
     1021 tggtgagggc ggtgcctgtg gggttgttgg ggtagcacag gtagatgagg tccaccttct
     1081 gtgatggtaa ctctggtatg aagttgttct cagaggtgca gggcaggtac accaggttct
     1141 ggtagtgacc atcatcatcc atgggtccgg tgcggccagc catgacgttg ctctccacgt
     1201 agaccgggta caccgggtcg gtcactgcca cggtgttgtc cagtccgaag atctcctgga
     1261 tgttgccggt gtcgcacttg gccccgtcac tgatgaacac ctcatccggt gatagctgga
     1321 tgcccagggc ctggtagtcg tgtttgatga tgtcctcaac taggaaatgg tagccctgtt
     1381 caggcccgta gcccatgaag ctatccttct cccccatctc ttccactgct tctttaaatc
     1441 cttccaccac tgcccggggc aggggcaggg tcacgtctcc gatgcccatc ctgattaccc
     1501 gggcttccgg gttttctttc tcgtactttt ccaccctctg ggctatttct gaaaagatgt
     1561 agctgctttt tagaagcagg tagttctcgt ttattatcgc catactcatt ttctcctatc
     1621 ctattattta tgggcgtagt agaaggtcat cttgcccagg tacttctcat ccaggcaggc
     1681 aaatcccacc ctctcaaact ggaccacgtc tccctcttcc tgctggtagg tgtcggcctc
     1741 ggcgtaccct gtctgtacac tgccatcggg catcaccatc tccaccagtt tcttctcccg
     1801 catgggcacc cactgcacaa tggatgcacc gtgttcccgg gcttcctcca ggctggtgct
     1861 gtggtaacgg gcttctcctt cctggaaggt gatgttcacc gcgtccatga gccgcaccac
     1921 ctgccccggt ttttttggta tgtcagtgga ggacaggtag atcctgtcct cgaagttcag
     1981 ggtcctcata cccctttcag ggtggtccgg gtgcaggggc ctctccacag gccctttagc
     2041 ctccctgggc agtcccagga tcttaacttg cctggcgggg gtggccagga agtagcggtt
     2101 cacctcctcc agatggctac gattcaaacc atagatcttc ttccaagata ctgtggtgtc
     2161 agcgctttta actccaatct ccatcatgag ctcccggatg ctctgggcct gtatcccccg
     2221 gcgtctgacg gcccgcaggg ttcccaggcg ggggtcatcc cagccggtgt actctccctc
     2281 gtcgatgcct tttttagcct tcgaggtgga cagggccatg tcctccatct tgagccgtcc
     2341 ataatggttg tagtggggct cctcccagtc caggtgccgg tagaggtagc cctgcttctc
     2401 ggtgttggcc atgtggtcct tgccccgtag tacgtggct
//
LOCUS       DAOQVW010000220         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_53068_length_2430_cov_3.294737, whole genome shotgun sequence.
ACCESSION   DAOQVW010000220 DAOQVW010000000
VERSION     DAOQVW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53068_length_2430_cov_3.294737"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKK85_05405"
     CDS             complement(<1..429)
                     /locus_tag="PKK85_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNS25491.1"
                     /translation="MAEMKRALGLFDVINLVIGTIVGADIYIAAAFGAGLLGPASLLA
                     WVLAGVMALVIALCFAECSSLTPRVGGPYAYAREAFGDFAGFMVGWSLIIASWAAIAV
                     FPLAFVAYLTYFFPHIVPWQQDLIKIAFVFFLTFINYRGVK"
     gene            475..1590
                     /locus_tag="PKK85_05410"
     CDS             475..1590
                     /locus_tag="PKK85_05410"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNS25492.1"
                     /translation="MMKPSQRLESIDLSGVRKMFDLVGEGTINLALGEPDFNTPSHIK
                     DSVKRALDENFTHYTGNQGTLELREAISEKLRQDNHIETSPENVIVTVGASEALLMST
                     LALVDKGDEVLIPDPGFVSYQACVELAGGTTVPVELDCEDDFRLNPENVQEKISQKSK
                     AIIMNSPANPTGGVMKKEDVKGLAEIAQDHDLVIISDEIYEKIIYGSKHYSPGRYTKN
                     AITINGFSKAYAMTGFRVGYLSAPSNLSESLLKVHQYSTACASSLSQAAALEALTGPQ
                     DSVGEMVAEFQRRRDLVVSRLNGMGIECKLPQGAFYLFPQVRDPELFVSEAIKEGVVL
                     VPGASFGAHGAGHFRLSYATSYPELLTAMDRLELLEW"
     gene            1749..>2430
                     /locus_tag="PKK85_05415"
     CDS             1749..>2430
                     /locus_tag="PKK85_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS25493.1"
                     /translation="MWEEVKLEGMPGLSQEEASAKLKSEGYNELPSPDKRTLFAIALE
                     VVREPMFLLLIACGSIYLVLGDLEEALMLLAFVFVIMGITFYQERKTENTLEALRDLS
                     SPRALVIRDGQQKRIAGREVVTGDIIMLQMGDRVPADAVVLSCSNLLINESLLTGESA
                     PVRKVSCGGILDMHPPGGDSLPSVFSGTLVVQGQGVAQVMKTGLQTEMGRIGKSLQSL
                     ETENTALQK"
BASE COUNT          634 a          635 c          647 g          514 t
ORIGIN      
        1 cttcactccc cggtagttta taaaggttaa aaagaatacg aaggctattt tgatgaggtc
       61 ctgttgccag ggcacgatgt ggggaaagaa gtaggtgaga tatgccacga atgccagagg
      121 aaatactgcg atagctgccc aggaggcaat tataagggac cagcccacca tgaatcctgc
      181 gaagtcccca aaggcctccc tggcataggc gtaggggcct cctacccggg gagttaggga
      241 ggagcattca gcaaaacaga gtgctatgac tagggccatt acaccggcta aaacccaggc
      301 taaaagagat gccgggccca gtaaacccgc cccaaaggcc gcggcgatgt agatatcagc
      361 acctactatg gtgccgataa ccaggtttat cacatcaaaa agaccaaggg cccgcttcat
      421 ctcagccatg ctttccttta tattagtcta ggacgataaa taattaaggt aatgattatg
      481 aagccatctc aacgtctaga atccattgac ctctccgggg ttcgtaagat gttcgacctg
      541 gtgggtgaag gtaccatcaa cctggccctg ggcgaacccg acttcaacac ccccagccat
      601 attaaggact ctgttaaaag ggccctggat gagaatttca cccactacac cggtaaccag
      661 gggaccctgg aactccggga agccatatct gaaaagctaa ggcaggataa ccacattgaa
      721 acttccccgg aaaatgtcat agtcaccgta ggtgccagtg aggcccttct gatgtcaacc
      781 ctggccctgg ttgataaggg agacgaagtc ctcatccctg atccaggctt cgtatcctac
      841 caggcctgtg tagaattggc tggaggcacc actgtacctg tggagcttga ctgtgaggat
      901 gacttccgat taaaccccga gaatgttcag gagaagatca gccagaaaag caaggccatt
      961 atcatgaact ccccagccaa ccccaccggg ggagtcatga aaaaggagga tgttaagggc
     1021 ctggccgaga tcgcccagga ccatgacctg gtgatcataa gcgacgagat ctacgagaag
     1081 atcatctacg ggagcaagca ctacagtccc ggacgataca caaaaaacgc catcaccatc
     1141 aacggcttct ccaaggccta cgccatgacc ggattccgtg tcggatatct atccgcacca
     1201 tccaacttat cagaatctct cctgaaggtg caccagtaca gcacagcctg tgccagttct
     1261 ctctcccaag ccgcagcact ggaagccctt accgggccgc aggatagtgt gggtgagatg
     1321 gtggcggagt tccagagaag aagagacctg gtggtttcca gacttaatgg tatgggcatt
     1381 gaatgtaagc tgccacaggg agctttctac ctcttccccc aggttaggga tcctgagttg
     1441 ttcgtgtctg aagcaattaa ggagggagtg gtactggtgc caggtgcttc cttcggggcc
     1501 catggtgcgg gtcacttccg tctctcctat gcaacgtcct acccggaact gctaactgcc
     1561 atggatcggc tggagctcct ggagtggtaa acaccatttt taaaaaaaaa actccattaa
     1621 cttggcctaa ttcttgatca tcaagctaca cattcttttg gcactacttt gggctctgtt
     1681 aattccagat aaaaatataa gtactcattt tttcatactt ttaaaaaccc aaggagtggg
     1741 agaaaaagat gtgggaagag gttaaactgg agggaatgcc gggactatcc caggaggagg
     1801 ccagtgctaa attaaagtcc gaaggctata acgaactccc ctccccggat aaaagaacct
     1861 tgttcgccat tgccctggaa gtagtgcgcg aacccatgtt ccttctttta atagcctgtg
     1921 gcagcatcta tctggtactg ggggacctgg aggaggccct gatgctcttg gccttcgtgt
     1981 tcgtgattat gggcatcacc ttctaccagg aacggaaaac cgagaacacc ctggaggccc
     2041 tgcgggatct gtccagcccc cgggccctgg taatcaggga tggacagcag aaacggatcg
     2101 ccgggaggga agtggtcacc ggggatatca tcatgctcca gatgggggac agggtaccag
     2161 ccgacgccgt ggtcctctcc tgcagcaacc tcctcatcaa cgagtcgctt ttaaccgggg
     2221 aatcagcacc ggtgcgtaag gtctcctgtg gtgggatcct ggacatgcac cccccaggtg
     2281 gtgacagcct cccatcggtg ttctccggga ccctggtggt gcaggggcag ggagtggccc
     2341 aggtaatgaa aaccggcctc cagacagaga tgggccggat aggtaagagt ttacagtccc
     2401 ttgaaaccga gaacaccgcc ctccagaagg
//
LOCUS       DAOQVW010000221         2425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_53231_length_2425_cov_3.759494, whole genome shotgun sequence.
ACCESSION   DAOQVW010000221 DAOQVW010000000
VERSION     DAOQVW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53231_length_2425_cov_3.759494"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..672)
                     /locus_tag="PKK85_05420"
     CDS             complement(241..672)
                     /locus_tag="PKK85_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNS25494.1"
                     /translation="MSFSTVIAVFTASVLELWLAIPLGLALGLNPVVTALTTMAGSIV
                     AVLVVILAGDNLRTHFIRWKYGDKKQIHSSGLNQIWSSYGVVGLGLFSPLLFGAPLGS
                     VVGIALGAEKKPLMFWMSLGIVIWSLGLTWAGFIGMLNLQS"
     gene            complement(682..969)
                     /locus_tag="PKK85_05425"
     CDS             complement(682..969)
                     /locus_tag="PKK85_05425"
                     /inference="COORDINATES: protein motif:HMM:NF022420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HNS25495.1"
                     /translation="MDLFEELMDELENLSEEDHSSRIKELEGDCVCPICPTYEDCGKG
                     ENIFCITQKSSCIDMELGCLCPTCPLAQKYKIGVLHNFYCHRGTEKEQRKG"
     gene            complement(1122..1511)
                     /locus_tag="PKK85_05430"
     CDS             complement(1122..1511)
                     /locus_tag="PKK85_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNS25496.1"
                     /translation="MENLLKRHEIKTTPQRRELIRILEELGNTHPSFNEIYRAVKAVH
                     PNISRSTVYENLKLLVELEAIKSFHYQGEIRYEMDPEPHVNLAGPGGLIRDIKNPEIL
                     QHLEDIMKILKKEQGINLKSLLVIASE"
     gene            1697..2263
                     /gene="afpA"
                     /locus_tag="PKK85_05435"
     CDS             1697..2263
                     /gene="afpA"
                     /locus_tag="PKK85_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeoflavoprotein AfpA"
                     /protein_id="HNS25497.1"
                     /translation="MEKRKKKVAWGITGAGDKILETLEVMKNIKTAYEDDVDIEVYIS
                     KSGDQVVKYYGISNQIEENFDRIWVEINANAPFLAGNIQLGKYKFMLIAPATSNTVAK
                     LSMRMGDTLLANAAIMGQKADVPLYILPSDYEEGVTITKLPNGKDLKITIRKEDVEHV
                     QKLAKMHNTFIIKNPEDIKEVFSQQFPI"
BASE COUNT          776 a          514 c          442 g          693 t
ORIGIN      
        1 cttctccaca taccatctta aaaaatggtg cctaggattc gttatttaaa atcaatatca
       61 ccattctaac taataagccg tttaatttct cttagggtaa ccttagccta gatttactat
      121 taaatgtttc gatcaactgg ccatgtaatc ttataaaact tagaaaaaca ctttatcggt
      181 ctttttttat ccttgcaaac cttatggatc ccatcaaaaa aaatggtgta ccttaactca
      241 ttaactttgc agatttaaca ttccaatgaa cccggcccag gtgaggccca aactccagat
      301 cacgatccct aaactcatcc agaacatcaa aggcttcttt tctgcaccta acgctatccc
      361 caccacagaa cctagaggtg caccaaaaag aaggggagag aataacccca accccactac
      421 accataactg ctccatatct ggttcaaacc actgctgtgg atctgttttt tgtccccata
      481 cttccatcta ataaaatggg tcctcagatt atcaccagcc aggatcacca ctagaaccgc
      541 aacaatggaa cctgccatag ttgtcaatgc tgtaaccact ggatttaacc ccaaagccaa
      601 gcccagtggg atagccagcc ataactccag cacactagct gtaaaaactg ctattaccgt
      661 actaaaactc atatctatta attaaccttt tctttgctct ttttcagtcc cacggtgaca
      721 gtagaagttg tgaagcaccc ctattttata tttctgtgcc aggggacagg tgggacacag
      781 acaacccagt tccatatcta tacagctact tttctgggtg atgcagaaaa tgttctctcc
      841 cttgccacag tcctcataag tggggcagat gggacaaaca cagtcaccct ccaattcttt
      901 gatcctgctt gaatgatctt cttcactcaa attttccagt tcatccatta gttcttcaaa
      961 tagatccatt tgtatcacct tgaattaggt attataattt gaataatatt aattggggtt
     1021 gatggggggg gaacttgggt catggattaa aataattttt aattccattt ggatcatttc
     1081 ttgacatgaa aggaaaactt aaacgagaac ttctaactta tctattcgct agcaatgacc
     1141 agcaatgatt taagatttat tccttgctct tttttcagta tcttcattat atcctctagg
     1201 tgctgcaaaa tttcaggatt ttttatatct cgtataagtc caccaggccc tgccaggttt
     1261 acatggggtt caggatccat ttcgtatcga atttctccct ggtaatggaa gcttttaatg
     1321 gcctccagtt caaccagaag tttaagattc tcgtagactg tcgagcggct tatattaggg
     1381 tggacagctt taacagctcg ataaatttca ttaaaggagg gatgggtgtt tcccagctct
     1441 tctagtattc gaatcaactc cctacgttgg ggagtggttt ttatttcatg tcgctttaac
     1501 aaattctcca tgaatagata gtttatttac atcaaataaa aaattatcga aacaaaactt
     1561 atatagaacc cagttctatc taaaaatagg gttttaaaaa aatagcaaca actgccagac
     1621 aatggaaccg gattaaataa ataaaatttg tcggagggaa aattttttac caataaaggc
     1681 tatcagggga aaaaatatgg aaaaaaggaa aaaaaaggtg gcttggggga ttaccggcgc
     1741 cggtgataag atcctggaaa cactggaagt gatgaagaac ataaaaactg catacgaaga
     1801 tgatgtggat attgaggtgt acatctccaa atctggtgat caagtagtga agtactacgg
     1861 gatatccaac cagatcgaag aaaacttcga taggatctgg gttgaaatca atgccaacgc
     1921 gcctttttta gcaggtaata ttcagttagg taaatataaa ttcatgttaa ttgcacctgc
     1981 cacctccaac accgtggcca aactatccat gagaatgggc gacaccctac ttgcaaacgc
     2041 ggccataatg ggccaaaaag ctgatgttcc actttatatt ctcccatcag attatgaaga
     2101 aggtgtcacc atcaccaaac tgcccaatgg taaggattta aagatcacca tacgaaaaga
     2161 ggatgtggaa catgtgcaaa aactggctaa aatgcacaat acatttataa taaagaaccc
     2221 ggaagatata aaagaagttt tctcacaaca atttcccatc taaatcgatt taaaacctga
     2281 attacttgaa aataagatca tttccaatat tggagaagaa aggtttgtgg atggtgaaag
     2341 ccacactcgt ggtggaatcc actactgtcc attcgatccc ggaaaagtgg gtctagtact
     2401 acaattcact gagggcttct agggg
//
LOCUS       DAOQVW010000222         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_53882_length_2408_cov_3.495963, whole genome shotgun sequence.
ACCESSION   DAOQVW010000222 DAOQVW010000000
VERSION     DAOQVW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53882_length_2408_cov_3.495963"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKK85_05440"
     CDS             <1..313
                     /locus_tag="PKK85_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNS25498.1"
                     /translation="PENFTGTIKKETPSHILLVDAVNMNKAPGFVRLVSKEEISNYSI
                     STHAIPISFLINYLEMGTGAKIALIGIQPMNMEIGHEMTMDVKKSAINVLNMLKTILL
                     P"
     gene            442..1557
                     /locus_tag="PKK85_05445"
     CDS             442..1557
                     /locus_tag="PKK85_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNS25499.1"
                     /translation="MKLMFIGSRLFEDVALYTKNKGITSVLTESNPDAPNQNLADVLH
                     RVPRGMDGPKTVAIKEDVDGVVPLIGVDGPLLSLSSLKEDLEENYGIPVVTSPLEAAS
                     IAQSKFKTKRFLTENNFPTPPFQSIDMNLSESYFSFPMVLKQSHGQGGVGIEVVNSHH
                     EMIEYLKKFGNALAEEFIAGVEISVEVLGWKGDYIPLVPVYKGKTSTKGTHPLFKVKK
                     APLEIEGLDNSKNNKMILELAREICRMMGVAGSADLDLIFDGKENLVLEINTRPSGTR
                     YLTYASCNINPLQEMVDMAAGKWNPEKVQYNLKNYFAMEVPVGDFSTKRNSYLYRNFP
                     DSNGWIIHGPPQHQRITIRGETRNSVLKIAQSLKLPS"
     gene            1710..>2408
                     /locus_tag="PKK85_05450"
     CDS             1710..>2408
                     /locus_tag="PKK85_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNS25500.1"
                     /translation="MNELQTMILLFLLTFLATMFFTFFMRKILRDADITDSPIVTEHR
                     HKAGTPTMGGIAMLLGMLVGAAVFFTQMNLILTVIIMMGSGLVGLLDDLLGLKVKELQ
                     KVVRNISTQSVEIGRLTLKPGEEARVTTEKAKSDLAKLVPGEKVEVIGEVPIKSEVSE
                     RNKIIAQIVVALFLVATGAVSSGVLGFELGILIIPVLIFGVIGSINSVNLIDGMDGLA
                     VGILTIASLSCAIFT"
BASE COUNT          763 a          455 c          503 g          687 t
ORIGIN      
        1 tccagaaaac tttactggta ctataaaaaa agaaactcct agtcacattc ttctggtaga
       61 cgcagtaaat atgaataaag cccctggatt cgtaagattg gttagtaaag aggaaatatc
      121 taattacagc atctcaacac acgccatacc catatctttc ctgataaact atttggaaat
      181 gggtactggc gccaagatag cccttattgg aattcaacct atgaatatgg agataggtca
      241 tgagatgacc atggatgtaa aaaaaagtgc aataaatgtt ttgaatatgt taaaaaccat
      301 tcttttacct tgatttttta taccaattgg ttaaacggtt ccaaattcac caataaaacc
      361 cattaaatac cagttactcc aaatatccta gacatatctg ggcttagttt aaatttgaat
      421 ctgaaattat ctgtgattga aatgaaatta atgttcattg gctctcgtct gtttgaggat
      481 gtggcattat acaccaaaaa taaaggtatt acttcagtat taactgaatc taatccagat
      541 gctcccaacc aaaacctggc agatgtcctc caccgggttc ctcgaggaat ggacggccct
      601 aaaaccgtag ccattaaaga agacgtagat ggtgttgttc cattaatagg tgttgacgga
      661 cctttattgt ctctttcaag tttaaaagaa gatttagaag aaaattacgg gattccagta
      721 gttacttcgc cattagaggc tgcatctata gctcaaagca aatttaaaac aaaaagattc
      781 cttacagaga ataactttcc cacccctcct ttccagtcga ttgatatgaa tctatcggaa
      841 tcctacttca gtttcccaat ggtacttaag cagagtcatg ggcagggtgg cgtggggatc
      901 gaagtggtca atagtcacca tgaaatgata gaatatctga aaaaatttgg aaacgccctg
      961 gcagaagagt tcatagcggg tgttgaaatt tcagtggaag tactgggttg gaagggtgat
     1021 tacattccat tggtacctgt ttacaaggga aaaaccagca ccaaaggcac tcatcctctt
     1081 ttcaaagtta aaaaggcacc cctagaaatt gaaggcttag ataattccaa gaataataag
     1141 atgatccttg aattagcccg tgagatatgc aggatgatgg gcgtagctgg ctccgctgat
     1201 ttagatctca tatttgacgg gaaagaaaac ctggtcttgg agatcaacac ccggcccagc
     1261 ggaacccgtt acttgaccta tgcttcctgt aatattaacc cgctccagga gatggtggac
     1321 atggcagccg gaaaatggaa cccagaaaaa gtgcagtata atcttaagaa ttactttgct
     1381 atggaagtac ctgtgggtga tttttccacc aaacgcaaca gttaccttta caggaacttc
     1441 cctgattcaa atggctggat aatacacggc ccccctcaac atcaaagaat caccataagg
     1501 ggggaaacta gaaattcagt gttaaaaatt gcccaatcac tgaaattacc atcataaatt
     1561 gagtaaccat tatcgtctta gatattaaca tatatactaa aagtgaacaa attcggagtg
     1621 taaaatttaa gtaatatggc atggtttaag tatcatggta atgggatgtt gaatactttt
     1681 ataattataa tacataattt ggtggtagat tgaacgagtt acaaacaatg atcctactat
     1741 ttcttttaac cttcctggcc acgatgttct ttaccttctt tatgagaaag attttgaggg
     1801 atgcagatat tactgacagt cccatcgtta ctgaacaccg ccataaagct ggaactccca
     1861 ctatgggcgg catagccatg ttactgggca tgcttgtagg tgctgcagta ttttttactc
     1921 agatgaatct gattttaaca gtcataatta tgatgggctc cggtttggtg ggtctactgg
     1981 atgatctttt aggtttgaag gttaaggaac tgcagaaggt ggtccgcaac atcagcactc
     2041 agtcggttga aattggtcgc ttaactctca agcccggaga agaagcaaga gtaaccactg
     2101 aaaaagctaa aagtgatctt gcaaaactcg taccaggtga aaaggtggag gttattggag
     2161 aagtgcccat caaaagtgaa gtctctgaac gcaacaaaat aatcgcccaa atcgtggttg
     2221 cattgttcct ggttgctacc ggagcagtga gcagcggagt tttaggtttt gaattgggaa
     2281 tattaattat acccgtgctc atctttggag ttatcggttc aatcaattct gtaaacctca
     2341 tagatggcat ggacggtttg gctgtaggaa ttttaaccat agcatcgctt tcgtgcgcta
     2401 tatttaca
//
LOCUS       DAOQVW010000223         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_54507_length_2391_cov_2.885702, whole genome shotgun sequence.
ACCESSION   DAOQVW010000223 DAOQVW010000000
VERSION     DAOQVW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54507_length_2391_cov_2.885702"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..441)
                     /locus_tag="PKK85_05455"
     CDS             complement(76..441)
                     /locus_tag="PKK85_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HNS25501.1"
                     /translation="MEIPQNIQHQLAQFQQMQQQAQAISVQKQNVDLQTRETEKALEE
                     LKKANDDDDVYKTAGNLLIKVEKVSLTEELEEKLETLQLREKTIKRQEERVMKKLQEM
                     QESIQEAMQTTGIQPGMGN"
     gene            complement(499..750)
                     /locus_tag="PKK85_05460"
     CDS             complement(499..750)
                     /locus_tag="PKK85_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HNS25502.1"
                     /translation="MILQGVEIQIEVELDGQEEAEIVYGALKPEIKSSPSSRTRSWVK
                     IKDKKLILEVEARDSTSLRAAVNSYLRWINLSCQILELS"
     gene            complement(747..1241)
                     /locus_tag="PKK85_05465"
     CDS             complement(747..1241)
                     /locus_tag="PKK85_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Brix domain-containing protein"
                     /protein_id="HNS25503.1"
                     /translation="MLITTSRRPSPRTRSFARALERVLPSKYLNRGKMSLREVFLKGK
                     ELGFSPLMVISERDGNPSRLEFFTSNEKPEAYILINVDLSKPRGKIDTENLTFHCEEP
                     ELSHLLSKYLKIKLVNENYSDKYNRIWIKTGPAQRTIMEFYDNKGNLIQPRIYLREWK
                     EVVW"
     gene            complement(1247..1378)
                     /locus_tag="PKK85_05470"
     CDS             complement(1247..1378)
                     /locus_tag="PKK85_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HNS25504.1"
                     /translation="MYKCSKCGTLVDVKGYTEAKCPSCRYRILFKEIPPVRRSVKAR"
     gene            complement(1379..1648)
                     /gene="rpl37A"
                     /locus_tag="PKK85_05475"
     CDS             complement(1379..1648)
                     /gene="rpl37A"
                     /locus_tag="PKK85_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37Ae"
                     /protein_id="HNS25505.1"
                     /translation="MARTKKVGITGRFGPRYGRKAKKTVRTIEENMKKKHLCPQCDRP
                     AVKRVSRGIWKCKKCNTVFTGGAYMPATPVGKTAARNIKRIVGGL"
     gene            complement(1703..>2391)
                     /gene="rrp42"
                     /locus_tag="PKK85_05480"
     CDS             complement(1703..>2391)
                     /gene="rrp42"
                     /locus_tag="PKK85_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HNS25506.1"
                     /translation="VETGVIKKAESSARVKIGNTQIMIGIKPQIGEPFPDTPNVGVFM
                     TNSELVPMAAPNFEPGPPDERSVELSRVTDRGIREGKIIDLEKLAIIPGKKVWMIFID
                     MHILDYDGNLMDAAALGAMAALMDTKIPTATVEDDEVVIDHENMQPLPLNDKTVMTTM
                     AKIGNELVVDPSLEEEGIMGARISIGVRSDGAICSMQKGGERPFKKEEILKAIDLAQE
                     KTNELFKYIP"
BASE COUNT          556 a          578 c          457 g          800 t
ORIGIN      
        1 tggacaggat gtggtcctgg gcggcttgag ccgccgcagt ggaaagttca tccaattcaa
       61 ttgccgtgag tttcctcaat tacccatccc cggttgtatt ccggtagttt gcattgcttc
      121 ctgtatggat tcctgcattt cttgcagttt tttcataacc cgttcttcct gacgctttat
      181 ggttttctct ctgagttgga gtgtttccag tttttcctcc agttcctcag ttaaagaaac
      241 tttttcaact tttataagca agttaccagc agttttgtaa acgtcatcat catcattagc
      301 tttttttaat tcttctaatg ctttttcggt ttcacgagtc tgtaaatcca cgttttgctt
      361 ctgcacggat attgcctggg cttgttgttg catttgctgg aactgggcca gttgatgttg
      421 aatattttgg ggtatctcca ttctatcacc tcaatctttt aaaaatgttc agtgtttcta
      481 tcgatcctat tacacaaatt agcttaactc cagtatctgg caggataaat tgatccacct
      541 taaataagag ttaacagccg ctcttagcga ggtggaatcc ctggcttcca cttccagaat
      601 gagtttttta tcctttatct tcacccaact ccgggttcta gatgaaggtg aagattttat
      661 ctctggtttc aaggcaccat acactatttc agcctcttcc tgtccatcta gttcaacctc
      721 aatttgaatt tctactccct gaagaatcac caaacaacct ctttccattc cctcaaatat
      781 atcctgggct ggattaagtt tcccttatta tcataaaact ccattattgt tctttgagcc
      841 gggccggtct ttatccaaat tcgattgtac ttgtcactat aattttcgtt aaccaacttt
      901 atcttaagat atttactcaa taagtgggaa agttccggtt cttcacaatg gaaagtcaaa
      961 ttttcggtat caatcttccc cctcggctta gaaagatcca cattaattaa gatatatgcc
     1021 tcgggtttct cattgctggt gaaaaactcc aggcgactgg gattcccatc tctctccgaa
     1081 ataaccatca atggagagaa gccaagttct ttacccttca ggaaaacttc ccgtagactc
     1141 attttccccc ggttaagata tttggagggt aaaacccttt ccagggcgcg ggcaaagctc
     1201 ctggttcttg gtgatggtct ccgagaggtg gttattaaca tgccatttat cgggctttaa
     1261 ctgaccttct aacaggagga atttctttaa atagtatcct ataacggcag ctaggacatt
     1321 tagcttctgt gtatcctttt acatccacta aagtaccgca ttttgagcac ttgtacaact
     1381 ataatcctcc aacgatcctt tttatgttac gggcagcggt tttacccacc ggggtggcgg
     1441 gcatgtaagc tcctccagta aatacggtgt tgcacttttt gcacttccat attcctcgag
     1501 aaaccctctt tacagcaggt cggtcacact gaggacataa gtgctttttc ttcatatttt
     1561 cttcaatggt cctcacggtt ttcttagcct tcctaccata acgaggacca aatctgccgg
     1621 taatgccgac tttcttggtt cttgccatta atatcacctt aaattttata ttctactctt
     1681 ctttcaccta agggttcaaa acttagggga tgtacttgaa tagttcattg gtcttttcct
     1741 gggccaggtc aatggcctta aggatttctt ccttcttgaa aggcctttca ccaccctttt
     1801 gcattgagca gatggccccg tcactacgga caccaattga tattcgagcc cccataatcc
     1861 cttcttcttc cagtgaagga tctaccacca gttcgttccc tatcttagcc atggtagtca
     1921 tgacggtctt gtcgttaagg ggtaatggct gcatgttttc gtgatcaatt accacttcat
     1981 catcctccac tgtagcagtt gggatcttgg tatccattaa agcagccatg gcccccaatg
     2041 cagcggcatc catgaggttt ccatcgtaat ccaagatgtg catgtctatg aagatcatcc
     2101 acaccttctt tccgggaatt atggctaatt tttccagatc aatgatctta ccttccctta
     2161 taccacggtc agtgaccctg gaaagttcca cggaacgttc atcagggggg cctggttcaa
     2221 agtttggagc tgccatgggt accagttcag agttggtcat aaaaactccc acattgggag
     2281 tgtcagggaa tggttcgccg atttgtggtt tgataccaat cattatctga gtgttgccta
     2341 ttttgacccg ggccgaactc tctgcctttt ttattacccc ggtttccact g
//
LOCUS       DAOQVW010000224         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_54559_length_2389_cov_3.943016, whole genome shotgun sequence.
ACCESSION   DAOQVW010000224 DAOQVW010000000
VERSION     DAOQVW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54559_length_2389_cov_3.943016"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PKK85_05485"
     CDS             <1..783
                     /locus_tag="PKK85_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25507.1"
                     /translation="GESIKDMDVYPHYVSSAEIDDETCIYCKACETACPREAITIARD
                     LPDRAKLVTGEIEIDKETCIDCGICEEMCPADAITLEHKIPTSADPTVSSDINVDKDK
                     CVYCLICRKVCPVDAIKAVCRTCSYGEYDLDPAAAEIEGSSFIDDDLCVRCGWCQEIC
                     PVDAAKVKKPFKGELTIDQDKCTSCGACVDICPCDVLSFPQPEEPGQIVDKVFKDEKY
                     CIYCGACGNVCPVEAITVKRTDVDYTPTKSKSWKNKMESLKT"
     gene            800..1030
                     /locus_tag="PKK85_05490"
     CDS             800..1030
                     /locus_tag="PKK85_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25508.1"
                     /translation="MELKVNQDNCLGCGVCVVACPVNVSISPEVAGGHGPKTEEVVMM
                     VENGVIKLFTDANCTKCGTCQLFCPVDAIWLE"
     gene            1040..1570
                     /locus_tag="PKK85_05495"
     CDS             1040..1570
                     /locus_tag="PKK85_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNS25509.1"
                     /translation="MTYIDKPVVPKVVKLDEPTAKERKLLNMMLNTGSDIYQGACKKR
                     GSTCKDEYRKVCGVSYMDPKDMAKLGVANWDSVKVTTDWGEVVTSAVHSRDAPHEGTI
                     FIPKGPWANVITSHETYCCCDPTYKGIYCTVEKSDEEVLLMADLMRAVYKKYVKDEES
                     ISELESLGEKPVFKKK"
     gene            1583..>2389
                     /locus_tag="PKK85_05500"
     CDS             1583..>2389
                     /locus_tag="PKK85_05500"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HNS25510.1"
                     /translation="MAYEPPVTDYDEIVENCTCAFCGCNCDDLDCLVKDGHVVAVRHA
                     CRLGASKIMEDMDQRLLVPMVRDENGDLVEVDWDTALDKAAELIAGAVRPIFYGWSET
                     SIETMKYGIRLGELVGAVLDNQATICHGPSLQAVQNVGYPVATLGEVKNRADMIVYTG
                     SNPMNSHPRHLSRYSTFPRGWFRQRGRFDRTLVTMDPKYSDTAKMSDIWVGFEQNGDY
                     GFYNAIRAALKGKEIDQDFVSGIPKEDIMELAEAMKNVQFGALYFGLGLTH"
BASE COUNT          752 a          485 c          579 g          573 t
ORIGIN      
        1 ggtgaatcca taaaagacat ggatgtctat ccacattacg tgtcttctgc cgaaattgac
       61 gatgaaacat gcatatattg taaagcctgt gaaacggctt gtccccgaga agcgatcacc
      121 atagcccgtg atttgccaga tagggctaaa ctggtaactg gtgaaataga aatcgataag
      181 gaaacttgta tcgattgtgg tatatgtgaa gagatgtgtc ccgctgatgc cattactcta
      241 gaacacaaga taccaacatc tgcagatcct actgtatcgt ctgatataaa cgtggacaaa
      301 gataaatgcg tctactgtct tatctgccgc aaagtatgtc ctgtggatgc cattaaggct
      361 gtttgtagga cttgttccta cggagaatac gacctggatc ctgcagctgc agaaattgaa
      421 ggaagttcat tcattgacga tgatttatgc gtaagatgtg gatggtgcca ggaaatctgc
      481 ccagttgatg ctgccaaggt aaagaaacca ttcaagggag agctaaccat agaccaggac
      541 aagtgcacaa gctgtggagc gtgtgtggat atttgtccct gtgatgtttt atccttcccc
      601 caacctgaag aaccgggaca aatagtggat aaagtattca aggatgaaaa gtattgcata
      661 tactgcggtg catgtggaaa cgtatgccca gtagaagcta ttaccgtcaa gagaaccgat
      721 gttgactata ctccaaccaa atccaagtca tggaagaaca agatggagtc tctaaaaacc
      781 taagatttgg gtgataatga tggaactgaa agtaaaccaa gataactgcc tgggatgtgg
      841 tgtctgtgta gtggcctgcc cagtaaatgt atctataagt cctgaagtag caggaggaca
      901 cggacccaaa actgaagaag tagtcatgat ggtggaaaac ggagtaatca aactcttcac
      961 cgatgctaac tgcaccaaat gtggaacctg ccaactgttc tgccccgtag atgctatatg
     1021 gctggaatga ggtgaaaaaa tgacttatat agacaaaccc gtcgttccca aagttgttaa
     1081 attagatgaa ccaactgcca aagaacgaaa actattgaac atgatgctga acaccggctc
     1141 agacatctac caaggggcat gtaaaaaaag aggttcaacc tgtaaagatg aataccgaaa
     1201 ggtatgtgga gtatcataca tggaccctaa ggatatggcc aaattaggtg ttgcgaactg
     1261 ggatagtgta aaagtaacaa cggattgggg tgaagtagtg acttctgctg ttcactccag
     1321 agacgcacct cacgaaggaa ccatattcat acccaaaggt ccctgggcca atgtgattac
     1381 aagccacgaa acctactgtt gctgcgaccc cacctacaag ggtatctact gcacagtaga
     1441 aaaatcggac gaagaagttt tattgatggc tgacctcatg agggcagtct acaaaaaata
     1501 tgtaaaagac gaggaatcca tttctgaatt agaatcactt ggtgagaagc ccgtctttaa
     1561 aaagaaataa ggaggcctta aagtggcata cgaaccacct gtaactgatt atgatgagat
     1621 cgtagaaaac tgcacctgcg ccttctgcgg atgcaactgc gacgatttgg attgcctagt
     1681 taaagatgga catgtagttg ccgttcggca tgcctgtcga ctgggagcca gtaaaatcat
     1741 ggaagacatg gatcagagac tcttagtccc catggtccgt gatgaaaacg gtgatcttgt
     1801 ggaagttgac tgggacactg ccctcgacaa agcagccgaa ctcatagctg gcgcagtacg
     1861 accaatattc tatggctgga gtgaaacttc cattgaaact atgaagtatg gtatccggct
     1921 tggtgagcta gtaggagctg tactggacaa ccaggcaaca atctgccatg gtccatcact
     1981 acaagcagtt caaaacgtag gataccctgt tgcgaccttg ggtgaagtaa aaaaccgtgc
     2041 agacatgatt gtttacactg gaagtaaccc gatgaactca caccctcgac acctttctcg
     2101 atacagcacc ttcccccgtg gatggttcag acagagggga cgatttgacc gtacattggt
     2161 aacaatggac cctaaataca gtgacactgc taaaatgtcc gatatatggg ttggatttga
     2221 gcaaaacggt gattatggat tctataacgc aataagggcg gccttaaaag gaaaagaaat
     2281 tgatcaagac tttgtttctg gaatacctaa agaagacatc atggaattag ctgaagccat
     2341 gaagaatgtt cagttcggag cgttatactt tggactgggc ttgacccac
//
LOCUS       DAOQVW010000225         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_54856_length_2381_cov_3.510748, whole genome shotgun sequence.
ACCESSION   DAOQVW010000225 DAOQVW010000000
VERSION     DAOQVW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54856_length_2381_cov_3.510748"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..553
                     /locus_tag="PKK85_05505"
     CDS             128..553
                     /locus_tag="PKK85_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25511.1"
                     /translation="MDQKGFIFTFDAVLALIPLVLMMVLISNLPVSGDSPSQLTISQD
                     AQDYLNVLASSQIKDGKVMDWMVQALKTGNNDGVEEAGKLAKPILDNLTSGRSYQLLE
                     TGQLNNTILISQGNLNTSTIVGSGTLCWENFIFVLYVGQ"
     gene            746..1225
                     /locus_tag="PKK85_05510"
     CDS             746..1225
                     /locus_tag="PKK85_05510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNS25512.1"
                     /translation="MRIIPGHKTCHYGELLVETEYWLVLLAPDQRNLGTCIVALKRDE
                     NSLSSLKKKEWIEFVEIVSILESALKKAFPVTLFNWGCHLNSRYQRDHTDCHLHWHLI
                     PRYKNPVQLGGHTFQDPCFGESTMKATVGSFFPSEDLKGEIVTRIREHLKDLNRKKA"
     gene            1268..1339
                     /locus_tag="PKK85_05515"
     tRNA            1268..1339
                     /locus_tag="PKK85_05515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Arg,seq:gcg)
     gene            complement(1351..>2381)
                     /locus_tag="PKK85_05520"
     CDS             complement(1351..>2381)
                     /locus_tag="PKK85_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNS25513.1"
                     /translation="TSNALQKTGDGYALAYRAGADLLDMEQVQFHPTGMLYPDSRRGV
                     LVTEAVRGEGGRLINALGERFMTNYDPRGELATRDVVARSIYNEIREGRGTKRGGVYL
                     DVSHLPSELIEEKLETMLFQFLDVGLDIREEPMEVAPTAHHVMGGSLITSKCETTISN
                     LYAAGEAAGGVHGANRLGGNALAETQVFGKRAGEAAAVNARKRSVAINKKQLDAEEDR
                     ISGTFKEGDYYPHQLKEMLQSLMWDKVAIIRNQTGLARALGLIQELKTKMKDLTVPDG
                     KGFNNYLLDALELENMLQVAELVTHSAIIRKESRGSHYREDYPKTLKNWNRSIVMNKD
                     APVRFIER"
BASE COUNT          633 a          534 c          519 g          695 t
ORIGIN      
        1 caaccagaag atttccaaga gatgaaaaag aatgtggaaa aggcagcgtt agaagctata
       61 gcaaaaaaag acaaagtagt ggacttgatc aggaaaaaat aaatgaggga gtatgggcag
      121 gacattcatg gatcaaaagg gctttatttt cactttcgac gctgtcttag cccttatacc
      181 cctggtttta atgatggttc tcatcagtaa cctgcctgta tcaggggatt ccccatcaca
      241 attaaccatc tcccaagatg cccaggatta tttaaatgtt ttagcatcca gtcagataaa
      301 agacgggaaa gttatggact ggatggttca ggccttaaaa accgggaaca atgatggagt
      361 ggaagaggct gggaaactag ccaaacctat cctggataat ctaaccagtg gtagaagcta
      421 ccagcttctg gagacaggcc agctaaacaa cacaatactc atttcccagg gaaaccttaa
      481 cacttcgacc atagtcggct ctggtactct atgttgggaa aattttatct ttgtactcta
      541 cgttggccaa taaacatgat aatctggttg atctgggttt aaagtgttat cttaatggac
      601 tcatcctggt aaagccataa tcagtgccag ttctatgatt cggtttaagt gtcaacttga
      661 ttccctgtaa attgggttgc tagtaacttt tttttggtgt caatttattt tacttcaaaa
      721 taacaaaata tatcctgtat ggagtgtgag aattattccc gggcataaaa cgtgccatta
      781 cggcgaactc ctagtagaga ctgaatactg gctggtgttg ctggctccag atcagcgtaa
      841 cttgggcaca tgtattgtgg ctcttaaaag agatgaaaac agtttatcca gtttgaaaaa
      901 aaaagaatgg atagaattcg ttgaaattgt gtcaatttta gagtcagcac ttaaaaaagc
      961 atttcctgtg accttgttca actgggggtg ccatttaaac tccaggtatc aaagggatca
     1021 cactgattgt cacctgcatt ggcatttaat accccgttat aaaaatccgg tgcaattggg
     1081 tggtcatact tttcaggatc cctgctttgg tgagagcacc atgaaagcta cagtgggttc
     1141 ttttttcccg tctgaagatc ttaagggaga aatagtgact aggatcaggg agcaccttaa
     1201 agatttaaat agaaaaaagg catagtatca taagcacacc ttggtgttgg tgtactctaa
     1261 ttttttcgcc ctgatagtgt agcggatatc acgtaggatt gcggatccta ttacccgggt
     1321 tcaagtcccg gtcagggcat gcactttttt ttatctttca ataaatcgta ccggggcatc
     1381 tttattcatt acgatacttc tattccaatt cttcagggtt tttgggtagt cctcccgata
     1441 atgggagccc ctgctttctt tccgtattat ggctgagtgg gtaactaact ctgccacctg
     1501 cagcatattt tcaagctcca gtgcatccag caagtagttg ttgaatccct taccatccgg
     1561 cacggttaaa tctttcattt tagttttaag ttcctgtata aggccaaggg cacgtgctaa
     1621 tccagtttgg tttctgatga tggccacttt atcccacatc aaggattgga gcatttcttt
     1681 caattgatgg ggatagtagt ccccttcctt aaatgttcca gatatcctat cctcttccgc
     1741 gtctagctgt tttttattaa tggcaaccga tcttttcctg gcattaactg ctgctgcttc
     1801 accagccctt tttccaaaga cttgggtttc ggccagggca tttcctccca ggcgattcgc
     1861 cccgtgaact ccacctgcgg cttctccagc tgcatagagg ttgcttatcg tggtttcaca
     1921 tttagaagtg attaaagaac cccccatgac atggtgggct gtaggtgcta cttccatggg
     1981 ctcttcacga atatccaaac ccacatccag aaattggaag agcatagtct ccaatttttc
     2041 ttctattagc tccgatggga ggtggctgac atccaggtat actccaccac gtttggtacc
     2101 tcttccctcc cgaatctcat tgtaaatgga acgagccaca acatcccggg tagcaagctc
     2161 accccgggga tcgtagttgg tcatgaaacg ctcacctaga gcgtttatca gccgtccgcc
     2221 ttctcctcga actgcttcgg taaccagcac ccccctccgt gaatcaggat acaacatgcc
     2281 tgtaggatga aactgcactt gttccatgtc cagcagatct gctccggcac ggtaggccag
     2341 ggcataccca tccccagtct tttgcagggc attggaggtt a
//
LOCUS       DAOQVW010000226         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_55268_length_2371_cov_2.328152, whole genome shotgun sequence.
ACCESSION   DAOQVW010000226 DAOQVW010000000
VERSION     DAOQVW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55268_length_2371_cov_2.328152"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PKK85_05525"
     CDS             complement(<1..522)
                     /locus_tag="PKK85_05525"
                     /inference="COORDINATES: protein motif:HMM:NF021400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2101 family protein"
                     /protein_id="HNS25514.1"
                     /translation="MGIFAKFGDFIIKLFSFVGMLILSIPKIPEKIRNINTSGIRDKI
                     DTESVKGNISRIRSEVGGVKDTYSRENYSRPEHARTSGESGAHLEDPGVILISGTYTS
                     EEKERTILILQIASAAFIVVSIFYVFNFLSLILFLILGGLIGAFVIYMLYKRVTKMYR
                     RDFNAYRDFFLLYL"
     gene            complement(782..1234)
                     /locus_tag="PKK85_05530"
     CDS             complement(782..1234)
                     /locus_tag="PKK85_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HNS25515.1"
                     /translation="MDSRGQASAEYIFLILIFLIVLALVTVPLAGRTINATQNVSVTS
                     DAKTALSTIANGVNVVYSNGPGAKRTVNVYFPQDSSLFYDDDGTLNLGLRDIPVNASN
                     PDVKKDTVYLDVPYEVQVDGTVTKGWHDVEITWDVGSTYILVSIKPSP"
     gene            complement(1302..>2371)
                     /locus_tag="PKK85_05535"
     CDS             complement(1302..>2371)
                     /locus_tag="PKK85_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HNS25516.1"
                     /translation="NKLTFPLIGVGLILNAIYAVLIGNLLLIVSAAIITAVIFALGYL
                     FWKMGAWAGGDVKLFTALAALLAVYPPIINYNILNYQMPFYSTYPFPFTLIINSILSM
                     LPFLLIYVVYIGFKHKPKVLEELTAPLKDYRKNMVQALVVSSAVYIAYFITFYLPFRN
                     LLLSMVLIIILSLVISKLPKLIKAVLVSVVMVLSLIQNLQVTILGFIVLFIIMTVTAV
                     VRKLMTKASKEALQDDYKINELKEGMISAYNLYQKGDEVYYDDKSFFDKIREAVKTGD
                     IFLLSTPKGKLLVSTLAAGLKNEDIELLKKLSSEGKISDTFRVKKGVPFAPSILIGLV
                     ISLLIGDLVIILLGVLNWLLY"
BASE COUNT          766 a          473 c          433 g          699 t
ORIGIN      
        1 caggtacagc aaaaagaaat cacggtaggc gttgaaatcc ctccggtaca tcttcgttac
       61 ccttttgtac agcatataaa tcacgaaggc accgataaga ccgcccagta tcaggaagag
      121 aattaaggat aggaagttga acacgtagaa aattgaaacc acgataaagg ccgctgaagc
      181 aatttgtaga attaaaatag ttctttcctt ctcttctgaa gtataggtcc ctgaaattaa
      241 gattacacct ggatcttcaa ggtgagctcc agattccccc gatgttctgg catgttccgg
      301 tctggaatag ttctctctgg aataggtgtc cttcacacca cctacttcac tcctgatccg
      361 ggatatgttg cccttcacag attcagtgtc gattttatcc cggatcccac tggtatttat
      421 attccttatc ttctctggaa tcttggggat ggatagaatg agcattccca cgaaggaaaa
      481 tagtttgatg ataaaatctc cgaatttggc gaatataccc aacttgaaat ctccattcaa
      541 atttatttga ataaaattat tacactattt ttgaataata attataacgc aatataatat
      601 aaattaattg gtattttaaa ggtgactatt aaattaatcc ctggattaag tattttaaag
      661 gtgactatta aaatatttca gtggattata gaatttaaaa tgaattcgat atttaaaaat
      721 aagcattaaa ggattataac tatttacaga tccttcccta cttgcaatga tattttactt
      781 tttacgggga tggtttgatt gaaactaata tataggtact tccaacatcc catgttattt
      841 ccacatcgtg ccaacctttg gtaacagtac catccacctg aacctcgtag ggcacgtcca
      901 gataaacagt atccttttta acatccggat tactggcgtt gactggaata tctcgaagac
      961 ctaaatttaa agtaccatca tcgtcgtaga ataaactact gtcttgcggg aagtacacat
     1021 tcacagttct tttagcaccg ggtccattgg agtacaccac gttaacccca ttagctatgg
     1081 ttgaaagggc ggttttagca tcagaagtta cggagacatt ctgggtggca ttgatggtcc
     1141 gtccagctaa gggaacggta actagagcta gaactatcag gaaaatcagt ataagaaata
     1201 tgtattctgc agatgcctgt cccctactat ccataactcc tcacatcaat gtatgattta
     1261 aacttattac cttataaatt ttggtgtcac cgccagttta tttagtacaa caaccagttc
     1321 aacacgccca aaagtatgat gaccagatcc cctattaaaa gcgagatcac cagaccaatt
     1381 aagatggaag gtgcaaaggg aacacccttc ttcaccctga aggtatctga gattttacct
     1441 tcactggaaa gcttttttaa gagttctata tcctcatttt taagaccggc tgccagtgtg
     1501 ctcaccagta atttaccctt gggtgtactc agcaggaaga tgtcaccagt ttttacagct
     1561 tcccttattt tatcgaagaa actcttatca tcataatata cctcatcacc cttctggtaa
     1621 aggttgtaag cagatatcat tccctccttg agctcgttaa ttttataatc atcctgcaag
     1681 gcttccttac tggctttggt cattaatttt cttacaactg cagttaccgt cattattata
     1741 aataagacta taaatccgag gattgtaact tgtaaattct gtattaagga caaaaccata
     1801 acgactgata ccaggacggc cttgatcagt ttaggtaatt tagagataac cagtgataat
     1861 ataattatca gtaccatact caacaggaga tttcggaagg gcaaatagaa agtgatgaaa
     1921 taagcaatat acactgcaga agagaccacc agagcctgaa ccatgttctt gcgataatcc
     1981 tttaatggag cagttaattc ctccagaacc tttggcttat gcttaaatcc tatataaacc
     2041 acatagatta aaaggaaagg taacatggaa aggatactgt ttataatcaa tgtaaatgga
     2101 aagggatagg ttgaataaaa aggcatctga tagtttaaaa tattgtaatt aattatggga
     2161 ggataaacag caagtaatgc cgccagggca gtgaatagtt tgacatcccc accggcccag
     2221 gcacccatct tccagaacag gtaccctaaa gcaaagatga ccgcggttat gatggctgcg
     2281 cttacaataa gcagtaaatt accaattaaa actgcatata tagcatttaa aattaatccc
     2341 acccctatca ggggaaaggt aagtttgtta g
//
LOCUS       DAOQVW010000227         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_55334_length_2369_cov_2.773552, whole genome shotgun sequence.
ACCESSION   DAOQVW010000227 DAOQVW010000000
VERSION     DAOQVW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55334_length_2369_cov_2.773552"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1278
                     /gene="proS"
                     /locus_tag="PKK85_05540"
                     /pseudo
     CDS             1..1278
                     /gene="proS"
                     /locus_tag="PKK85_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295809.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
     gene            1291..1971
                     /locus_tag="PKK85_05545"
     CDS             1291..1971
                     /locus_tag="PKK85_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25517.1"
                     /translation="MNIRAMILVLLVVVFAVAMGAINFASLGSVDLEKAQSQGTAWVI
                     QNTAAGTVPHTVTITNNGTQAVRVNQGQLLESPDSQDLVVAENMTISPGSNGTVQAYC
                     TEPAQKATPGSSLKANQAANPMVLFIIQSSTPTDAASARQAQLKIWSLKTGGEVDPYT
                     GEAAAMVKTSKSSYYQLRQDLSTAREDLSSQFKLSNESLPSLADQRDGLTRWLEGLGN
                     WFKEFLGT"
     gene            1982..>2369
                     /gene="cofC"
                     /locus_tag="PKK85_05550"
     CDS             1982..>2369
                     /gene="cofC"
                     /locus_tag="PKK85_05550"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNS25518.1"
                     /translation="MKKTAAIIPVSRFSHAKTRLSPSLSPSEREGLLKAMLQDVILAL
                     QNVVDMVVVISSDGEVLNYLHPLDVHCLKEEGETDLNGALTQALEWYQPRGDQVIIVP
                     SDVPLLSEGLVRGLLAMAEEYQVVIAP"
BASE COUNT          598 a          635 c          674 g          462 t
ORIGIN      
        1 tctgccgcac ggcttcccgc aggtcgctga aattgatgaa gtactctttc cattactaat
       61 acccgaagat gaactggcca aggaggccat ccatgttaag ggctttgaag aagaggttta
      121 ctggataacc cacgggggat taacccccct gggaaagaag ctggccctgc gtcccacagt
      181 gaaaccgcca tgtaccccat gttcgccttg tgggtgcgca gccaccagga tttacccctc
      241 cgcttctacc aggtggtgaa caccttccgc tatgagacca aacacacccg gcccctgata
      301 cgggtgaggg agatcaccac ctttaaggaa gcccacaccg tccatgccac aagccaggag
      361 gcagaagaac aggtagagag ggctgtagaa atatattcat ccttctttga cctgatgggc
      421 atcccctacg ttgtaacccg gcgcccagat tgggataagt tcccaggggc cgattacacc
      481 atggccttcg acaccctgct cccggatggt aagaccctgc agatcggaac cgttcataac
      541 ctggggcaga cctttgccca gaccttccaa atcacctacg aaaccgagga tggtcagcat
      601 gaatacgttt accagacctg ttacggcctt tcggaccgga tcatagcttc cattataggg
      661 gtgcatggtg atgaaggagg cctgtcacta cccccagcag tggctcccta ccaggtggtg
      721 atagtgccca tcatcttcaa ggaaggtgcc caggaagtcc tggacttctg cagccaagta
      781 aaaaaccagc tgagcagggc tggcatccgg gtgcatctgg atagccgtga cctacgggcg
      841 ggtaaaaagt tctacgaatg ggagatgagg ggagtgcccc tccgaataga gatcggcccc
      901 cgggagatca gggagggtaa gatggtcctc aagagaaggg acacccctga taaggagact
      961 gtggagtgcc gggaagatga actggcagag ctggtggagg ataggctggg aaggatcagc
     1021 agccacctta aagagaaggc ctggaatatg ctccaggaga acatgaaaga aacagactcc
     1081 ccactggagg cccgggacat catccaggag gatgggggtg tagtttcatt cctatggtgc
     1141 ggggatgaag cctgtggtaa agagctggag gaacaggttc acgtggatat cctgggtata
     1201 caggaagatg cagatgggac ctgcgttaac tgtaaacaga aggccaggta ccgggcgctc
     1261 ctggcccgga cctactagga ggatgtggag atgaacatca gggccatgat attggtactg
     1321 ctggtggtgg tgttcgcagt ggccatgggg gccatcaact tcgcctccct gggtagtgtg
     1381 gacctggaaa aggcccagtc ccagggcaca gcctgggtta tccagaacac cgctgcaggg
     1441 accgtgcccc atacagtgac cattaccaac aatggaaccc aggctgtgag ggtgaaccag
     1501 ggccagctat tggaaagtcc agactcccag gacctggtgg tggctgagaa catgaccatc
     1561 tcccctggta gcaacggcac tgtccaggcc tactgcactg aacctgcaca gaaagccacc
     1621 cctgggagta gtttaaaagc caatcaggct gctaatccca tggtactatt catcatccag
     1681 tcctcaactc ccaccgatgc tgccagcgcc aggcaagccc agcttaagat ctggagcctg
     1741 aagaccggag gggaagtgga cccctacact ggtgaggctg cagcaatggt taaaaccagt
     1801 aaatcatcct actaccagtt gcgacaggac ctctccacgg cccgggagga tctcagctcc
     1861 cagttcaaac tcagcaacga gtccctgccc agcctggcag accagcggga cggactcacc
     1921 agatggctgg aaggccttgg aaactggttt aaagagtttc taggaaccta ggggataaaa
     1981 tatgaagaaa actgctgcta taataccagt ttcacgtttt tcacatgcca aaacccggct
     2041 ttcaccatcc ctatccccat ctgagaggga gggactcctg aaggccatgc tacaggatgt
     2101 gatcctggcc ctgcagaatg tggtggatat ggtggtggtg ataagctctg atggagaggt
     2161 gctaaactac cttcaccccc ttgatgttca ctgtttaaag gaagagggag agacggatct
     2221 aaacggagcc ttaacccagg ccctggaatg gtaccagccc cggggtgacc aggtcatcat
     2281 agtaccctcc gatgtacccc tcctcagtga gggactggtc aggggcctct tggccatggc
     2341 cgaggagtac caagtggtca tagccccct
//
LOCUS       DAOQVW010000228         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_55466_length_2365_cov_4.590909, whole genome shotgun sequence.
ACCESSION   DAOQVW010000228 DAOQVW010000000
VERSION     DAOQVW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55466_length_2365_cov_4.590909"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PKK85_05555"
     CDS             <1..710
                     /locus_tag="PKK85_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNS25519.1"
                     /translation="TMILMIIGGTSFLVHYKALKGRVIDVFHDIQFQATIILVTLFSI
                     LLILVTKFTTIDAVFFVISALSCTGSNIQPVYIMNNWPDYAKIVITLCMVIGMSSGST
                     TGAIKLIRIITIVKGVYWEIIRIISPTGSVIPRKISGKPIGDVEIREASSYLFIYILF
                     LFISWLILMQCGYDGINSIFEIASAQGNVGLSMGITSPHMPEVAEIFLIMNMWVGRIE
                     IIPAIVLFKAFWDMLKR"
     gene            733..2166
                     /locus_tag="PKK85_05560"
     CDS             733..2166
                     /locus_tag="PKK85_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNS25520.1"
                     /translation="MRNYLDKRDLQIIANPLGIIMEGIGGVVLLPIIIALIYGEHHYI
                     VFLIFGVVSILLGFILRKIPSDYSKFTLKHAMVIAAIAWLWAALMGSFCLIHSTNVSF
                     LNAYFESMSAWTGTGISIYTDVEVLPKSILFLRSLEQWVGGLGVVIIVIGILLRPGTA
                     AARLYKSEAREEKIKPSITGTVKTIWLIYLFYTVVGILLYVAAGMPLFDAINNTFSTI
                     CTGGMSIKNDSIGAYNSIPIYIITMILMIIGGTSFLVHYKAFKGNVLDAIRDIQFQTA
                     IAIIVLFSILLIVVTKFSTVDAFYFVISALSTTGSSIQPVNTIAGWPNYAKIVIIAAM
                     ILGMSAGSTSGAFKLIRLITLFKGIYWEIIRIISPTGSVIPRKISGKPIGDVEIREAG
                     NYLFLYLSFICISWLMLMQHGYNGIDSLFEVASAQGNIGLTTGIISATMPPIAQVFLI
                     VNMWIGRIEIIPALVLVKLFIDLVKRI"
     gene            2239..>2365
                     /locus_tag="PKK85_05565"
     CDS             2239..>2365
                     /locus_tag="PKK85_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNS25521.1"
                     /translation="MYIVIMGGGRVGLTLASSLVTRGRDVTLIENDTNLCSNAAAE"
BASE COUNT          701 a          450 c          474 g          740 t
ORIGIN      
        1 tcaccatgat cctgatgatc attggcggaa caagcttttt agtacattac aaagccttga
       61 agggtagagt aatagatgtt tttcatgata tacagttcca ggccaccata atacttgtaa
      121 ccctgttttc tattcttctt attctggtca ccaaattcac caccattgat gcagtttttt
      181 ttgttatttc tgcacttagc tgcactggct ccaatatcca gccagtttac atcatgaata
      241 attggccaga ttatgctaaa attgttatta ctctatgtat ggtgattgga atgtcttccg
      301 ggtctactac aggggctatc aaacttatcc gaataattac aatagttaaa ggagtgtatt
      361 gggagatcat tcgcataatt tcgccaaccg gttcggttat cccccgaaaa atatcaggta
      421 aacccatcgg agacgtggaa atcagagaag caagtagtta tctctttatt tatatcttat
      481 ttttgtttat aagctggtta atactgatgc aatgtgggta cgatggtata aactccattt
      541 ttgaaattgc atcagcccag ggtaatgtag ggctttcgat gggtataacc tccccacaca
      601 tgcctgaagt tgctgaaatc ttcttaataa tgaatatgtg ggtaggtagg atcgaaatca
      661 tcccggcaat agtcttattt aaggccttct gggacatgtt aaaaagataa aaaaatataa
      721 aaaaagggta acatgagaaa ttatctcgat aaaagggatc tgcaaataat tgcaaaccct
      781 ctaggcatta taatggaagg aataggcggt gtagtactat tacccattat tattgcctta
      841 atctacgggg aacatcatta tattgtattc ttaatttttg gagtagtttc aatacttttg
      901 ggtttcattt taagaaaaat tccctctgac tactctaagt tcacccttaa acacgccatg
      961 gtaatagctg ccattgcttg gttatgggct gcgctaatgg gaagtttctg tttaatacat
     1021 tctactaacg tgagcttttt aaacgcttac ttcgaaagta tgtccgcatg gaccgggact
     1081 ggtataagta tttacactga tgtggaggta ctgcccaagt caattctatt tttaagaagc
     1141 ctggagcaat gggttggtgg cttaggagtg gttattatcg ttataggaat attacttaga
     1201 ccaggaaccg ctgctgctcg tctttacaag tctgaagcac gggaagaaaa aattaaaccc
     1261 agtatcaccg gtaccgtgaa aactatatgg ttgatctacc tattttacac ggttgttgga
     1321 attctactat acgtagcagc gggcatgcct cttttcgacg ccataaacaa cactttcagc
     1381 accatatgta ctggaggaat gtccattaaa aatgacagta ttggggctta caacagcatc
     1441 cccatctaca tcatcaccat gatcctgatg atcattggcg gaacaagctt tttagtacat
     1501 tacaaagcat ttaaaggaaa tgtactcgat gctatccggg atattcagtt tcaaaccgct
     1561 atagctataa ttgtcctatt ttcaattctg ctgatagtgg taactaaatt tagcactgta
     1621 gatgcctttt attttgttat ttcagcactt agtaccacag gttccagtat ccaacccgtt
     1681 aataccatag ctggttggcc aaactatgca aaaatagtta taattgccgc catgatcctg
     1741 ggaatgtctg ctggatccac tagcggggca tttaaactta taaggttgat taccctgttc
     1801 aaaggaattt actgggagat cattcgcata atttcgccaa ccggttcggt tatcccccga
     1861 aaaatatcag gtaaacccat cggagacgtg gaaatcagag aggcaggaaa ttatcttttt
     1921 ttgtatcttt cattcatctg tatcagttgg ttgatgctca tgcagcacgg atacaatggt
     1981 atcgactcac tctttgaagt agcatcggcc caaggcaaca ttggcctcac aactggaata
     2041 atatccgcta caatgccccc catagctcaa gtttttctga tagtaaacat gtggattggg
     2101 aggattgaaa ttattccagc actggttcta gtaaaacttt tcattgatct ggttaaaaga
     2161 atttaaaagg tgcaaaacaa aagctataaa aacatatcat tactggtcaa ataaaattac
     2221 aaccagaggt gtttatcaat gtacattgtt attatgggag gtggaagggt tggactgacc
     2281 ctggcatctt cccttgtaac ccggggacgg gatgttacac ttatagagaa tgatactaat
     2341 ctttgtagta atgctgctgc tgaat
//
LOCUS       DAOQVW010000229         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_55926_length_2353_cov_4.188425, whole genome shotgun sequence.
ACCESSION   DAOQVW010000229 DAOQVW010000000
VERSION     DAOQVW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55926_length_2353_cov_4.188425"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKK85_05570"
     CDS             complement(<1..559)
                     /locus_tag="PKK85_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HNS25522.1"
                     /translation="MSDLTSLGLKKGQLYETIVVTKNSDGSPNAAPMGLIVKSIQEVV
                     FYFYPGSRTAENVKKDGIFTVNILEDSLTFVECTLGSPPSSYFQKDGDLFHLKSADAY
                     FTARVVKEKVGPRKDRFGSTELTIIQALVEDVIKCVECVHPLNRAIYGIIEALVNLTR
                     MEMADQETRNVYLSRLPEISRLVNRV"
     gene            complement(567..1025)
                     /locus_tag="PKK85_05575"
     CDS             complement(567..1025)
                     /locus_tag="PKK85_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 family protein"
                     /protein_id="HNS25523.1"
                     /translation="MEQKEEFKGLTGNLTVFKNEVADAQKVTFAGVPGVCTPFAELFA
                     YAIRDKESVFVTGVDVETARKMEMTPHGVQLTDEADARADILVILGGLSMPKYNFELE
                     EIEKLKDQVLKDGGRMIGLSYMGMFQKVGWDEKLDFHCIIDGYLSGKIFR"
     gene            1327..1884
                     /locus_tag="PKK85_05580"
     CDS             1327..1884
                     /locus_tag="PKK85_05580"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HNS25524.1"
                     /translation="MKILVVNNHGQYNHRIYRSLHYLKIPAEIIPNSTSLEKVEEKDP
                     AGLILGGGPSLERSGNSSLYIKELDCPILGICLGHQIMAKTFGAEVKTAEIESYAQIE
                     INILRENDIFKGLGPSMKVWASHKDEVCALPEQFEVLADSYVCDIEAMKHLKKPLYSI
                     QFHPEVYHTPNGPRLFKNFYEVCKG"
     gene            complement(1930..>2353)
                     /locus_tag="PKK85_05585"
     CDS             complement(1930..>2353)
                     /locus_tag="PKK85_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25525.1"
                     /translation="WLVWVLLLLMGGSLIEGLFNKNCFNQYQKGIIILIFSISFLMAP
                     LTYMDTYKNSGEDIYQIQKSINLPRVYGRSASNGILDKSLYLSYYLGTNYAGQAGSKG
                     NYAGLENQLINSNVDLYFLWDDEAELNLENYTEIGKGE"
BASE COUNT          730 a          505 c          421 g          697 t
ORIGIN      
        1 ctacccgatt aaccaagcga gaaatttcag gtaatcggct gaggtaaaca tttctagtct
       61 cctgatctgc catttccata cgtgtaaggt taaccagagc ttcaataatt ccatatatgg
      121 cccggttaag ggggtgtaca cattctacac acttaatcac atcttccact agggcttgaa
      181 ttattgttaa ctccgtggaa ccaaatcgat ccttacgggg ccccactttc tcttttacca
      241 cccgggccgt gaagtaagca tcggcagatt ttaggtgaaa caaatcacca tctttttgga
      301 aataactaga gggaggggag cccaatgtac attccacaaa agttaaagaa tcttccaaga
      361 tattcaccgt gaagataccg tcctttttta cattttccgc tgttcgtgaa ccaggataaa
      421 aataaaaaac cacttcctgt atacttttaa caataagacc cattggcgca gcgttgggag
      481 atccgtctga attttttgtt acaactatcg tttcatatag ctggcccttt ttgagaccca
      541 gggaagtaag atcagacatt ttagatctat ctaaagattt ttccagataa atatccatct
      601 attatacaat ggaagtccag tttctcatcc catcctacct tctggaacat gcccatgtag
      661 gataagccta tcatcctccc cccatctttc aagacctgat cttttagttt ttctatttct
      721 tccaactcaa aattgtattt gggcatggat aagcctccta gaataaccag aatatcggcg
      781 cgggcatcag cttcatcagt taactgcact ccatggggtg tcatttccat cttccgggca
      841 gtttcaacat ccacccccgt aacaaaaacc gattccttat cccggatggc gtaggcaaaa
      901 agttctgcaa atggtgtgca gacccctggt accccagcga aggttacctt ctgggcatcg
      961 gccacttcat tcttaaatac ggttaaattt ccggttagtc ctttaaattc ttctttttgt
     1021 tccataatat cacttcaagt taataaacca ctcctaaatg caccaataaa acaaaaaatc
     1081 tataaaaagt tatttgatga gcttttgagg tatatgtata tcagatatgc caccaccaga
     1141 ataattataa tgggcagtaa ccagatgaaa acgtagaaaa aaaccactgc cagtagcagt
     1201 gccaccacaa taaatactat atcttgaagc tccatattct tttttttagt aactgagacc
     1261 ataaatatat tcctaaagta aatatttttg gtgatccgct tattgtattt ttagaggaga
     1321 tgaaaaatga agatattagt tgtaaacaac catggacagt acaaccaccg catttaccgc
     1381 agccttcact accttaaaat tcccgcggag atcattccca actctacttc actggaaaaa
     1441 gtagaagaaa aggatcctgc cggtttaata ctgggcggcg gcccttccct ggaaagatcc
     1501 ggaaattcca gcttatacat taaagaactg gactgtccca tactgggcat ctgcttagga
     1561 caccagataa tggctaaaac cttcggtgca gaagtgaaga ccgctgaaat tgaaagttac
     1621 gcccagattg aaattaacat attaagggag aatgatatat ttaagggttt aggcccatct
     1681 atgaaagtat gggcctcaca caaagatgaa gtgtgtgcat taccggaaca gtttgaagtg
     1741 ctagctgatt cgtacgtatg tgatattgaa gccatgaaac acctaaaaaa accattgtac
     1801 agcattcaat ttcatcctga agtttatcac accccgaatg ggccccggtt attcaaaaat
     1861 ttttatgaag tttgtaaagg ctaagaagga tgataaagtc cctatattct tgaataaatc
     1921 ttaacatcct tattcacctt ttcctatttc tgtataattt tctaaattca gttctgcctc
     1981 atcatcccat aaaaagtaaa gatccacatt ggaattgata agttgatttt ctaatccagc
     2041 gtagttacct ttggagcctg cttgtcccgc ataatttgtt cccaaatagt atgataggta
     2101 caatgattta tccaagattc cgttggaagc agatctgcca taaacccggg gaaggtttat
     2161 tgacttctgt atttggtaaa tatcttctcc tgaattttta taggtatcca tataggttaa
     2221 tggtgccatt aggaaagata tagagaaaat aaggattatt atcccttttt ggtattggtt
     2281 aaagcagttt ttattgaaca agccttcaat aagggagcct cccataagta aaagaagaac
     2341 ccagaccagc cac
//
LOCUS       DAOQVW010000230         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_55938_length_2353_cov_3.101828, whole genome shotgun sequence.
ACCESSION   DAOQVW010000230 DAOQVW010000000
VERSION     DAOQVW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55938_length_2353_cov_3.101828"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKK85_05590"
     CDS             <1..1020
                     /locus_tag="PKK85_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNS25526.1"
                     /translation="ENSVVTLDGSKGLVWEGKLVDTIKKEETTEQQPLMVAPPLLTVT
                     EVKVNVSMSEAAKKAAATGADGVGLLRTEHMMLTTGVHPKKYISEGNEDELIRVLVEN
                     ILKVADAFYPKPVWYRTLDAPTDEFQSLEGGEGEPHEHNPMLGWRGIRRELDEPDILR
                     AEFKAIKKLHEQGYTNIGIMLPLVQHPDELKEAKKIAREVGLKPQKTIEFGVMVETPG
                     AAMIIEDFIAEGLDFVSFGTNDLTQYTLAIDRNNEKVAGLYTEGHPAVLKLIERVIIE
                     CNKAGVKTSICGQAGSIPSIVEKLVELGITSVSANTDAVANVRETVARVEKKLLLRAA
                     RKMMQ"
     gene            1102..2247
                     /gene="mfnA"
                     /locus_tag="PKK85_05595"
     CDS             1102..2247
                     /gene="mfnA"
                     /locus_tag="PKK85_05595"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HNS25527.1"
                     /translation="MQDKGISREKVIKTLRDYKNRDLTYQSGRILGSMCTCPHPVALE
                     AYAMFLESNLGDPGLFPGTKDLEDEVITILGDMLGKSDVHGHIITGGTEANIMAMRAA
                     RNLSDVKDGEIIVPKSAHFSFKKAAEMLCLNLKEAELDENYRIDLNSVENLISDKTVA
                     VVGIAGTTELGKIDPIAGLSEICLDNDIYLHVDAAFGGFSIPFLQDAGYNLPEFDFKI
                     PGVSSMTIDPHKMGLAPIPTGGILFRHREYLESMSIETPYLTEDRQSTIVGTRTGAST
                     AATWALLKHLGRRGYRKIATRCMDLTHYLAESLEKADFILVTEPELNIVAFRSSQLTL
                     EEISRGMMDKGWLPSISAHPPAIRIIIMPHIRKNHLETFLADLKSIN"
BASE COUNT          716 a          512 c          581 g          544 t
ORIGIN      
        1 gaaaacagtg tagtgaccct ggatggtagt aaagggttgg tatgggaggg caaactggtg
       61 gacaccataa aaaaagaaga aaccactgaa cagcagccat taatggttgc accacccctc
      121 ttaacagtaa ctgaagtaaa agtcaatgtc agcatgtccg aagccgccaa aaaggccgct
      181 gccacaggag ctgatggtgt gggattgctt agaacagaac acatgatgct cacgactgga
      241 gtgcatccaa aaaagtacat ctcggagggg aatgaggatg aactcatcag ggtgctggtg
      301 gagaatatac tgaaagtagc agacgccttc taccccaaac cagtatggta ccgaactctg
      361 gacgcaccca ctgatgaatt ccagtccctg gaaggagggg aaggcgaacc acatgaacat
      421 aaccccatgt tgggctggag aggtatcagg agagagttgg acgagccgga tattctccgt
      481 gcagagttca aggctatcaa aaagctccat gaacagggat acaccaacat cggtatcatg
      541 ctgcctttgg tgcaacaccc cgacgaactg aaggaagcaa agaagatagc ccgtgaagta
      601 ggtctcaaac cccagaaaac aatagaattc ggggtcatgg tggaaacacc gggagcagct
      661 atgataatcg aggacttcat tgctgagggg ctggactttg tgagtttcgg aaccaacgac
      721 ctcacccagt acaccctggc catcgacaga aataacgaga aagtggctgg tttgtacact
      781 gaaggccatc cagcagtgct taagttgatt gaaagggtga tcatagagtg taacaaggca
      841 ggggtaaaga ccagtatttg tggacaggca gggagcattc cttccattgt ggaaaaactg
      901 gttgaactgg gaataacttc agtatccgct aacaccgatg ctgtggccaa tgtcagggaa
      961 actgtggcta gggtggagaa gaaacttcta ctccgggctg cccggaagat gatgcaatag
     1021 taggagtaga aaactccacc atattcctta ttttttaatt taatttccag agcccagaaa
     1081 aattctaata tttcagatca catgcaagac aagggaatct cccgtgaaaa agtcataaaa
     1141 actctccggg actacaaaaa tagggatcta acctaccaat ccggcagaat attaggatcc
     1201 atgtgtacct gcccccatcc agtggctttg gaagcctacg ccatgttttt agagtctaac
     1261 ctgggggatc ctggactttt ccctgggact aaagacttgg aagatgaagt gattactatc
     1321 ttaggggata tgctgggtaa atcagatgta catggccata tcatcacggg ggggactgaa
     1381 gctaatataa tggccatgcg ggctgcccgt aacctgagtg atgttaagga tggagagatc
     1441 atagtaccaa aatccgccca cttctccttt aaaaaagcag cggagatgtt gtgtcttaac
     1501 ctgaaagaag ctgaactgga tgaaaattac cgtatagacc tcaattcagt agaaaatctg
     1561 atctctgaca agacggtggc ggtagtgggg atagctggaa ccaccgaact gggaaaaata
     1621 gatcccatag ccgggttatc tgagatctgc ctggataacg atatttatct ccatgtggac
     1681 gctgcctttg gaggattttc catacccttc cttcaggatg ctggatataa tttaccagaa
     1741 tttgatttta agattccagg ggtttcatcc atgactattg accctcataa gatgggttta
     1801 gctcccatac ctactggggg aatcctattt cgtcataggg aatatctgga gtccatgagt
     1861 atagaaacac cctacctgac tgaggacaga caatccacca ttgtgggtac tcgaaccgga
     1921 gccagtaccg ctgcaacctg ggccctttta aagcacctgg gaaggagggg gtaccgaaaa
     1981 attgctaccc ggtgcatgga tttaactcat tacctggcgg aaagtctaga aaaagctgat
     2041 ttcatactcg taactgaacc agaacttaat atcgtggcgt tccgttcatc tcagctgaca
     2101 ctggaagaaa tatcccgggg aatgatggat aagggatggt taccttctat ttcagcacac
     2161 cccccggcaa ttcgcataat catcatgccc cacattagaa aaaatcactt ggagactttt
     2221 ctggctgatc ttaaatcaat aaattagaag aggaatttac attaagaagg ataaaattct
     2281 ccgcaccccc attggtaagg acatggtcca acaactgagg actggtgact tcatcaacat
     2341 ctctggtact atc
//
LOCUS       DAOQVW010000231         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_55970_length_2352_cov_3.742708, whole genome shotgun sequence.
ACCESSION   DAOQVW010000231 DAOQVW010000000
VERSION     DAOQVW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55970_length_2352_cov_3.742708"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKK85_05600"
     CDS             <1..633
                     /locus_tag="PKK85_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HNS25528.1"
                     /translation="VIRGTEKVDKRGGAALDLYSRDILKKADWKAALEYAKEIHEHVD
                     IRKVPKNRDRFGTVPVDPDAAYSAGTTLIGCDVGVNGDKLPELTKVGHRIYGEAGIHT
                     IFATLDHVSALVVKRLIKEAVEENVIEEGSVLGITGRAGITGRKPEFVMEYAKPYFKD
                     VLFVSDALALGAAVMARCMNSMGTTKIPLGGRQGGPCILGQRRRLQQKGI"
     gene            716..1312
                     /locus_tag="PKK85_05605"
     CDS             716..1312
                     /locus_tag="PKK85_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNS25529.1"
                     /translation="MVVAVIMAGGKGTRLRSNQEKPLLKVRGQPLIESVVNALLSSKK
                     VETILVATSVHSPQTAQWAKNKKLTNIDTPGRGYVEDMAFILSRPELLDKIILTVAAD
                     LPLLTGEVVDEVIHHYHVCKKSALSVMVPVEIFYKHCLQPSMVWENLVPSGLNILRGK
                     NTEQDEELLVLGKLEVALNINTPEDIISLERLWMNSCR"
     gene            1318..1590
                     /locus_tag="PKK85_05610"
     CDS             1318..1590
                     /locus_tag="PKK85_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNS25530.1"
                     /translation="MVEPERKLIKGEEKVWSEIKGYQVATNNARILGELEELIVNDRT
                     GKITDVVIKVDKGRNVQVKGAKKKGDNLLVPFGKVEKVGEFIIISE"
     gene            complement(1648..2139)
                     /gene="fae"
                     /locus_tag="PKK85_05615"
     CDS             complement(1648..2139)
                     /gene="fae"
                     /locus_tag="PKK85_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HNS25531.1"
                     /translation="MYLAGEALVGDGAEVSHIDLLIGDKEGPVGQAFANALANQVEKH
                     TPLFAVVTPNLVAKPITMLLPKVSIRDLDDATKIFGPAQKAVAMAVVDSVEEGIIPKE
                     MVEDICILCGVFIHPQAQDADKIYEYNYQATRISIKRAFASEPDINEIIEKKNLEGHP
                     FYK"
BASE COUNT          725 a          465 c          551 g          611 t
ORIGIN      
        1 gttatccggg gaacagagaa agttgataaa cgaggaggag cagccctgga tctgtactcc
       61 cgggacatac tgaaaaaagc agactggaaa gcagccctgg aatacgcaaa ggagatacat
      121 gaacacgtgg atatcaggaa ggtaccaaaa aacagggatc gtttcggaac cgtccctgta
      181 gatcctgatg cggcctacag tgctggaact acacttatcg gctgtgatgt gggggtcaac
      241 ggtgataagc ttccagaatt gactaaagtc ggtcatagga tatatggaga agcaggaatt
      301 cacaccattt ttgccaccct ggaccatgta agtgccctgg tagttaagag gttaattaaa
      361 gaggcagtag aagagaatgt catagaagag ggttctgttt taggcattac tggccgtgct
      421 ggtatcactg gccggaaacc agaattcgtc atggaatatg ctaaacccta tttcaaagac
      481 gtgttgtttg tatctgatgc cctggcttta ggggccgcag taatggctcg ttgcatgaat
      541 tccatgggaa ccaccaagat acccctgggt ggtagacaag gcggcccatg tattctcgga
      601 caacgccgaa gactacagca gaagggtatc tgaagcacct gcattaatgg aatatgatcc
      661 aaaaattcta taaccattca ggcctagctt caatcaagaa tctaagaggg aatccatggt
      721 tgttgcagtg ataatggctg gtggaaaggg caccagatta cggtcaaacc aggagaaacc
      781 ccttcttaaa gtaaggggtc aaccactaat tgagagtgtg gtaaatgctc ttttatcttc
      841 taaaaaagtt gaaacaatcc tggtggccac cagtgttcac agcccccaaa ccgcacagtg
      901 ggcaaaaaac aagaaattaa ccaatataga tactcctggc cgaggctatg tggaggatat
      961 ggctttcata ctttctaggc cagaacttct agataaaatc atcctcaccg tcgcagctga
     1021 cttgcccctt ctcactgggg aggtagttga tgaggtcatc catcattatc atgtatgcaa
     1081 aaaatcggcc ctctcagtga tggttccggt ggaaattttc tataaacact gtcttcagcc
     1141 cagtatggtc tgggaaaatc tggtaccatc tggattaaat atattaaggg gaaaaaatac
     1201 agaacaagat gaggagcttc tggtcctcgg caaattagaa gtagccttaa atattaatac
     1261 ccccgaggac ataatatccc tagaaagact gtggatgaac tcatgcaggt gatgtttatg
     1321 gttgaaccag aacggaaact cataaaaggg gaagaaaagg tatggagcga gattaagggt
     1381 tatcaagtag ctaccaataa tgcccgtatc ttaggtgagc tggaagagct catagtaaat
     1441 gatcgtaccg gtaaaataac cgacgtggta attaaagttg ataaagggcg aaatgtacag
     1501 gttaaagggg ctaaaaagaa gggtgacaac ctcttagtac cattcggcaa ggtggaaaag
     1561 gtgggagaat ttatcataat ctctgaataa tggtcccaca aaaatattaa ctatttttct
     1621 agtcttaaaa aaaaattaat aagggactta cttataaaaa gggtgtccct ctaagttctt
     1681 tttctcaata atctcattga tatctggttc acttgcgaat gcccttttaa tggagattct
     1741 tgtggcttga tagttgtatt catagatttt atccgcatct tgggcttgag gatgaatgaa
     1801 gacaccacag agtatacaaa tatcttcaac catctcttta ggtattattc cctcctctac
     1861 tgagtccacc actgccatgg ccacggcttt ctgggcgggt ccgaatattt tagtggcatc
     1921 gtctaagtct ctgatactca ccttgggcaa tagcatggtt atgggcttag ccaccagatt
     1981 gggagtaact acagcaaaaa ggggggtgtg cttttccacc tgatttgcaa gggcattggc
     2041 aaaggcttgg ccgactggcc cctctttatc tccaatcaga aggtcgatat gggacacttc
     2101 tgctccatct cctacaagtg cttctccagc aaggtacatg ttcaaatcac ctaatgtatg
     2161 ttttagtagg actcctataa taatttttca aaaaatattg gtaaaaacaa gtttcgtagt
     2221 atatgaaaaa aatggtgaaa gaaatgagag agaaaggaag gccatcacat tcctttcaga
     2281 ctcatatcaa tcatgcgctt ggtttcagaa gccagttctg gtggtaatcc agtgatatcc
     2341 atactaagga aa
//
LOCUS       DAOQVW010000232         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_56049_length_2350_cov_3.170806, whole genome shotgun sequence.
ACCESSION   DAOQVW010000232 DAOQVW010000000
VERSION     DAOQVW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56049_length_2350_cov_3.170806"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKK85_05620"
     CDS             <1..297
                     /locus_tag="PKK85_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS25532.1"
                     /translation="EIVSEQTLHNTSVGTEGDKWSWDSKNVLYVIKNHLQFSDKEVKD
                     VAATDLVIVTTKTTVKECANKLRQKKIEQMPVIDVEGDLVGLIRASDLIKSITD"
     gene            299..718
                     /locus_tag="PKK85_05625"
     CDS             299..718
                     /locus_tag="PKK85_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25533.1"
                     /translation="MSPVTLKIKESSEEIRIVAKNGSEVVIHPLNIKFLMATFWWEGT
                     PQLQTFFEILELTIKRTINQVFPHEKLILDYTYQANDALEEASLITVKINRVEADGQE
                     LEVEGDLISLHGNDDRGILRKITSHRRRVYENVVKEI"
     gene            complement(769..1812)
                     /gene="larE"
                     /locus_tag="PKK85_05630"
     CDS             complement(769..1812)
                     /gene="larE"
                     /locus_tag="PKK85_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNS25534.1"
                     /translation="MKLEDKLDQLKNYFQDHKVIIAFSGGSDSTLLAKIAERYSKEAL
                     AVTVDNGVMPAGFTKSAQEIARKLGINHLIIEDDLLENPDFSSNPPSRCYICKKNIHD
                     RIGEVLQEKGFDWVADGTNLTDLFEDRPGVEVNYQENIKIPLVEVGLSSMEVKELLKD
                     MKISYTPSTTCLATRIPSGSFLSMNKLRRIDYAEKFLKNLSDTDQLRVRDEDGTADIE
                     MDYHNLDNQTLSYIDSELKKIGFKKVTLNLAGYQTHKKDLVIYRPCQDSKNRIMFEAE
                     LPYPVDLEKSCEELKTIGTVKFSSDMGVALIKLDGVNITLFSKGKIVARNVKDRNHAE
                     DILYQIMPHIRRR"
     gene            complement(1824..>2350)
                     /locus_tag="PKK85_05635"
     CDS             complement(1824..>2350)
                     /locus_tag="PKK85_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HNS25535.1"
                     /translation="NPVIIGIIDGVFHQKPAVSHREIMDALDVGIKVVGGASMGALRS
                     AELEDMGMIGVGRVFQAYKNGLVESDDDVAVVFEPSSYEQLSEALVSMKYNFDKAVKK
                     GIISHEDLEKLYTTAKAIYYPKRNYNLVFRNSHLEDRKINSLKKFLAREGRDIKKQDA
                     MEVLNYIKKRINSF"
BASE COUNT          677 a          500 c          429 g          744 t
ORIGIN      
        1 gaaatagtgt ctgaacagac cctgcacaac acttcagtag gtactgaggg ggataaatgg
       61 tcctgggata gtaaaaatgt cctttatgtc ataaagaatc atcttcaatt ctcggataaa
      121 gaggttaaag atgtggctgc cactgactta gtcatcgtca ccaccaaaac cacagttaag
      181 gaatgtgcca acaaactgcg tcagaagaaa atagaacaga tgccagttat tgacgtggaa
      241 ggagacctag taggcttgat tagggctagt gatttgatta agtccattac cgattgaaat
      301 gtcccctgta accttaaaga taaaggaaag ttctgaagaa attcggatcg tggcaaaaaa
      361 tgggtcagaa gttgtcatcc atcccctgaa cataaaattc ctcatggcca cattttggtg
      421 ggaggggact ccccagctgc aaaccttttt cgagattttg gaactcacca taaaaaggac
      481 tattaaccaa gtatttccac atgaaaagtt gattctggat tatacctacc aagccaatga
      541 cgccctggaa gaagcctccc tcatcaccgt aaagatcaat cgggtggaag cagatggtca
      601 ggaattggaa gtggaagggg atttaataag cttgcatggc aacgatgatc ggggaatttt
      661 aagaaaaata actagccacc ggcgtagagt atatgaaaat gttgtaaaag agatttaata
      721 gcatttattt ttaaatctcc tgtttaatta ctccctatca gtttatactt atctacgacg
      781 aatatggggc atgatttggt agagaatgtc ctcagcgtga tttctatctt taacatttcg
      841 agccactatt tttcccttag aaaaaagagt aatgttcact ccatccagct ttattaaggc
      901 tacccccata tcgcttgaaa atttaacggt tcctattgtt tttagttctt cacaactctt
      961 ttccagatcc actgggtagg gtagttcagc ttcaaacatt atccggtttt tactgtcctg
     1021 gcagggcctg taaataacta gatccttttt atgagtttgg taccctgcca aatttaaagt
     1081 cactttctta aatccaattt ttttcagttc agaatcgata taactcaggg tttgattatc
     1141 taaattatgg taatccattt caatatctgc agtgccatct tcatcacgaa cccttaattg
     1201 atccgtatct gagaggtttt tcagaaattt ttcagcataa tcaattcttc ttagcttatt
     1261 catgcttaaa aaacttccag aaggtattcg ggtagccaaa caagtagttg atggtgtgta
     1321 agaaattttc atatccttca gtaattcctt tacttccata gagctcaatc ctacctccac
     1381 caaaggaatt tttatgttct cctggtagtt gacctccaca cctggcctgt cctcaaaaag
     1441 atcggtgagg ttggtgccat cagccaccca atcaaaacct ttctcctgca gtacttcacc
     1501 tatcctgtcg tgaatgtttt ttttgcatat gtaacagcga cttggtggat tagatgagaa
     1561 gtcgggattt tcaaggaggt catcttcaat aattaaatga ttgatgccta gttttctggc
     1621 gatttcctga gcacttttcg tgaaccccgc aggcattact ccattatcaa cggttactgc
     1681 caaagcctct tttgaatatc tttcagctat tttagccaga agtgtgctgt cagaacctcc
     1741 tgaaaaggca attatcacct tgtgatcctg gaagtaattt ttcaattggt caagtttatc
     1801 ttcaagtttc atggtcgccc agtttaaaaa gagttgatcc gttttttaat gtaatttaaa
     1861 acttccattg catcctgttt tttgatatcc cgaccttctc tggctaaaaa ttttttcaag
     1921 ctatttattt tcctatcctc caaatgggag tttcgaaaaa ccaagttata attccttttt
     1981 ggataataaa tagccttggc agttgtataa agtttttcta aatcttcatg agatattatg
     2041 ccctttttga cagctttatc gaagttatat ttcatactca ccagtgcctc agataactgt
     2101 tcatagctag atggttcaaa aaccacggcc acatcgtcat ctgattccac cagaccattt
     2161 ttatatgcct gaaaaactct tccaacacca atcatcccca tatcttctag ttctgctgac
     2221 cttaaagcac ccatgctggc accgcccacc accttgatcc ccacgtccag ggcatccatt
     2281 atctctcggt gggaaactgc tggtttctgg tgaaaaaccc catcaattat gcctattatg
     2341 accggattat
//
LOCUS       DAOQVW010000233         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_56227_length_2345_cov_3.548035, whole genome shotgun sequence.
ACCESSION   DAOQVW010000233 DAOQVW010000000
VERSION     DAOQVW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56227_length_2345_cov_3.548035"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PKK85_05640"
     CDS             <1..686
                     /locus_tag="PKK85_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNS25536.1"
                     /translation="PISGSDAIVSALVGYGLVSVGINSMNLNTLAFIVVSWIMSPLMG
                     LVTGFGVYYLLKNFLFKNITAISVKDRLEKIFSYLQIGSSAFSALNVGAIDIAVATGV
                     LYYAFGASVSQNIQILGAIGIVAGIMVAGGRITETIGRRITELVPSRGFSAQIAAATV
                     IFFFAGLGMPVSPTQTLVGTVIGIGLARGTSTVKVDVIKNIATTWILTIPACILISAS
                     MYLLYSILI"
     gene            1016..1171
                     /locus_tag="PKK85_05645"
     CDS             1016..1171
                     /locus_tag="PKK85_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25537.1"
                     /translation="MARVAECLEKYGFEVLEKYEFNYQPTTKELDQCYEIGIAVFIKL
                     MGEEWKR"
     gene            1179..1592
                     /locus_tag="PKK85_05650"
     CDS             1179..1592
                     /locus_tag="PKK85_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25538.1"
                     /translation="MKTMDYESPMDNEKKLMVVTFWVNRKAARTEGCAPFLIKKVTTS
                     QDTHLPDEGKFLKYSEDLIQEIASDLDDGETVEFIFNIGEEVMDVTLSNNSFSVEVTK
                     SPEIEEEIVDKLEMELRKKFPSLCDSFKPRVTPHE"
     gene            complement(1635..2030)
                     /locus_tag="PKK85_05655"
     CDS             complement(1635..2030)
                     /locus_tag="PKK85_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS25539.1"
                     /translation="MLKELKVKDVMTKDVITVPPQEDVIFAFEKLMKHRISSLPVMDG
                     GVLAGIVTATDLGHNLILDKYELGTTVEQVMIKDVVCVSPEDDLVTAVRKMNQYGKEE
                     GIINQLMVIEDHQIRGIISDGDIIRVLGL"
     gene            complement(2008..>2345)
                     /locus_tag="PKK85_05660"
     CDS             complement(2008..>2345)
                     /locus_tag="PKK85_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515278.1"
                     /note="catalyzes the formation of 2-phospho-D-glyceroyl
                     phosphate from ATP and 2-phospho-D-glycerate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HNS25540.1"
                     /translation="VEGVHIVPGFISQNLVSLSNVKMFVLTIQDEEVHRGRFYSRCRQ
                     MWARRPLKRYMDSFDDIRRTHLYFESQANKFHVPVIENIDVTTTIDSIIENITQTYGS
                     EEDVKGTQG"
BASE COUNT          604 a          530 c          564 g          647 t
ORIGIN      
        1 tacccatctc tggttcagat gccattgtaa gtgcactggt tggctatgga ctggtgagtg
       61 taggtattaa cagtatgaac ctgaatacgc tggcttttat cgtcgttagc tggatcatgt
      121 cacccctgat ggggctggtg acgggctttg gggtttacta tcttctgaaa aattttttat
      181 tcaaaaatat cacggccatc tctgttaagg accgtttaga gaaaatattt tcctacctgc
      241 agattggtag ctccgccttc tcagccctga atgtgggggc cattgacatt gccgtggcta
      301 ctggggtgct ttattatgcc ttcggagctt cagtcagcca gaatatacag atcctggggg
      361 ccattgggat tgtggccggg ataatggtgg ctggggggcg tataaccgag accatagggc
      421 gaaggatcac ggaacttgtt ccatcccggg gattctcagc acagatagcc gcagctacgg
      481 tgattttctt ttttgctggt ttggggatgc cagtttcacc aactcagacc ctggtgggca
      541 ctgtaatcgg gataggacta gcccggggta ccagtaccgt taaggtggat gtgattaaaa
      601 acattgccac cacttggatc ctgaccatac ctgcctgtat cctgatatcg gcctccatgt
      661 atcttctcta cagcatacta atctaagatc cctgtatcga aggaggctct aggtttcatg
      721 gaaaaaaata attaaaagtt caaatcaata agtaatagtt tctttaatca gccagtcccc
      781 aaccccagat tgtggtggct tgtatgggag gtataagatt ccagttaaga ttaaagaagc
      841 aatttactgg gtggagttct ggtcagtaac gcccttcaat tggaatcccc accaattaca
      901 aggacttcac cctagactag ggatactttg atgtactgga gggggtttta gcttcaaggc
      961 accggcttaa atggacaaag caccacctgg ggatagatag actggtggta aagccatggc
     1021 ccgagtggca gaatgcttag aaaaatatgg attcgaagtt ttagaaaagt atgagtttaa
     1081 ttatcagccc accaccaagg agctggacca gtgctacgag attggtatag ctgtctttat
     1141 caaactgatg ggtgaagaat ggaagaggtg aatggactat gaaaacaatg gactatgaaa
     1201 gccccatgga taatgagaag aagttgatgg tggttacctt ttgggtgaac cgaaaagcag
     1261 cacgtaccga gggttgtgct ccctttttaa taaaaaaagt taccaccagc caggacaccc
     1321 atctccctga tgagggtaaa ttcctcaaat attcggaaga tttgatccag gaaatagcct
     1381 ctgacctgga tgatggtgaa actgtggagt ttattttcaa catcggagaa gaggtgatgg
     1441 acgtcaccct tagcaacaac tccttcagtg tagaggtgac taaaagtccg gaaattgaag
     1501 aagaaatcgt tgacaaactg gagatggagc tccgcaagaa gttccccagc ttatgtgact
     1561 ccttcaagcc ccgggtaact ccccatgaat aggcagggcc ctccccctcc tttttttatg
     1621 aaaaaataat ctttttacag gcccagtacc cttataatat ctccatctga gataattccc
     1681 cggatctggt ggtcctctat taccatcagc tggttgataa tgccctcctc tttaccatac
     1741 tgattcatct tcctcacggc agttacgaga tcatcttcgg gactgacaca caccacatcc
     1801 ttgatcatta cttgctccac tgtggtgccc agttcgtact tgtccagtat caggttgtgg
     1861 cccaggtccg tggcagtgac aataccagcc agcacccccc catccatgac gggtagggag
     1921 cttatcctgt gcttcatcaa cttttcaaag gcaaaaatca cgtcttcctg gggtgggacc
     1981 gtaattacat ccttggtcat cacatcttta accttgagtt cctttaacat cttcttcact
     2041 tccatatgtc tgggttatgt tctcgataat tgagtcaatg gtggtggtga catcgatgtt
     2101 ctcgatgacc ggaacatgga atttgttggc ctggctttca aagtagaggt gggttctccg
     2161 gatatcatca aaggagtcca tatatctttt aagcggcctc cgggcccaca tctgtcggca
     2221 acgggagtaa aatcgtcccc tgtgcacttc ctcatcctgt atggttagga cgaacatttt
     2281 aacgttggac agtgacacta ggttttggct gatgaatccg ggtacgatgt gcaccccctc
     2341 tacca
//
LOCUS       DAOQVW010000234         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_56267_length_2344_cov_3.477064, whole genome shotgun sequence.
ACCESSION   DAOQVW010000234 DAOQVW010000000
VERSION     DAOQVW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56267_length_2344_cov_3.477064"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="gatB"
                     /locus_tag="PKK85_05665"
     CDS             complement(<1..795)
                     /gene="gatB"
                     /locus_tag="PKK85_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNS25541.1"
                     /translation="MICGLEIHVQLDTNSKLFCDCPTNYQEAAINHNICYVCFNQPGA
                     KPYPPNHEAIDGAIKIALMLGCKITPELTYFQRKHYDYPDLPSGYQRTSIPIGFEGDL
                     NGVRIREVHLEEDPGQYKPDLGIVDFNRSGIPLIEIVTEPDMTSPEEARKFLKELIRV
                     LEYSGSARGEGTMRADVNISLEGGKRAEIKNVNSIKGAYKALKFEMVRQKNLIKRGIE
                     VKQETRAFLESQMITVPMRLKEEADDYRYIPDPDLPPMLAEEEHVEY"
     gene            complement(838..1839)
                     /locus_tag="PKK85_05670"
     CDS             complement(838..1839)
                     /locus_tag="PKK85_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS25542.1"
                     /translation="MDLISKIKGFEILDMLQQSNQITLGRHKNIISLERAIFLSWWCD
                     KGDCAFCYMSTQKPLIKDPKKARRRFEAIMAEVEVVDRMGWNIEFLSGGYGSFTTPEI
                     RGVAQEIHQLTGDPVWLNVGITKDIDSYGEEVKGITGAVEVANPRLQRQICPSKNLDD
                     ILEMLELAGDLGFKKAITIILGLGETPDDLNYLFHLIEDYGINRIIFYSLNPHQGTPY
                     ALEPQPASLYYAGMVAATRIRFPDLEIITGTWIDNLANIGLLLMAGANGLTKFPLFSM
                     FGTGKGKRVEEEVRWAGRELDGTFTDSDRLVGPSYRRDLEPFVKRYIQRCHNNKSKQ"
     gene            2108..2180
                     /locus_tag="PKK85_05675"
     tRNA            2108..2180
                     /locus_tag="PKK85_05675"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2141..2143,aa:Asn,seq:gtt)
     gene            2185..2258
                     /locus_tag="PKK85_05680"
     tRNA            2185..2258
                     /locus_tag="PKK85_05680"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2219..2221,aa:Ile,seq:cat)
BASE COUNT          592 a          563 c          490 g          699 t
ORIGIN      
        1 gtattccaca tgttcctcct ccgccagcat ggggggcaga tccgggtcgg gtatgtaacg
       61 gtagtcatcg gcctcctctt tcaatcgcat agggacagtg atcatctgag attccaggaa
      121 agccctagtt tcctgtttca cttctatacc ccgcttaata aggttcttct gcctcaccat
      181 ttcaaattta agagccttgt aggcgccctt aatggagttt acatttttaa tttcagcccg
      241 cttaccacct tccagggaga tgttaacatc agcccgcata gtaccctcac cccgggcact
      301 cccactgtac tccaataccc tgataagctc ttttaagaat ttacgggcct cttcaggtga
      361 tgtcatgtcg ggttcagtta caatctctat caatggaatg ccagaccggt tgaaatccac
      421 aattcccagg tccggcttgt actgtcccgg atcttcctcc aggtgcacct ccctgatccg
      481 gactccattg agatctcctt caaagccaat ggggatggaa gttctctggt atcctgaggg
      541 taagtctgga tagtcataat gttttctctg aaagtaagtt aactccggag ttatcttaca
      601 gcctaacatc aaagcaattt ttatagcacc atctatagct tcatggttag ggggataggg
      661 cttagcacca ggttggttga agcacacata acagatattg tgatttatcg ctgcttcctg
      721 gtagttggta gggcagtcac agaaaagttt agaattggta tccagttgaa cgtgaatttc
      781 taatccgcat atcattttaa tggtgattcc tcctattcga gatgaaaagt ctgatcctca
      841 ttgttttgat ttattattgt gacaccgttg aatatatctt tttacaaagg gttccaggtc
      901 ccggcggtag ctgggcccca ccagccgatc agaatcagta aaagttccat ccagctccct
      961 cccagcccat cgaacttctt cttccactct tttacccttt ccagttccga acatggaaaa
     1021 aaggggaaat tttgttaatc cattagcacc ggccattaat aggagaccta tatttgccag
     1081 attatcaatc caagtcccgg tgataatttc cagatccgga aatcggatcc tggtggcggc
     1141 caccattcca gcgtagtaaa gagacgctgg ttgtggctcc aaggcatatg gggttccttg
     1201 atggggattc agtgagtaaa aaatgatccg attaatgcca taatcttcaa ttaggtggaa
     1261 aaggtagtta aggtcatcag gagtctctcc taatcccaaa attatggtta tggccttctt
     1321 aaaacccagg tccccagcca gttctaacat ttccagaata tcatccagat tcttactggg
     1381 gcatatttgt cgctgtaggc gaggattggc aacctccaca gcaccagtta tgcctttaac
     1441 ctcttcacca taggaatcaa tgtctttggt aattcccaca tttagccata ctggatcacc
     1501 agttaactgg tggatctcct gggccacacc tctgatctca ggtgtggtaa aagaaccata
     1561 ccccccagac aggaactcta tattccatcc catcctatcc actacttcta cctctgccat
     1621 tatggcttca aaccttctcc gggccttttt aggatccttg atgagtggtt tctgggtgga
     1681 catgtaacaa aaggcacagt ctcccttatc acaccaccaa gagaggaata tagcccgttc
     1741 cagggatata atatttttgt ggcgtcctag tgtaatctgg ttagattgct gcagcatatc
     1801 cagtatttcg aagcctttaa ttttgcttat gagatccata gtctccctaa atcgtggatg
     1861 tataatttat gctaaaagtt cacgtgaatg ccacctaaat ggtgattgga tgttgaaata
     1921 tattatttat catgaaaaga tttaatatgg agtatatttg aattgtttaa tatccttatt
     1981 gccaacccaa aatctttata taccattaat tactaccttc aaactacatc tcggtctgtt
     2041 atgtttttta ataaacttgt caactgagaa aatagtttgc aaatttagta ggctgtactt
     2101 gggatttgcc gccgtagctc agtaggtaga gcgttcggct gttaaccgat tggtcacagg
     2161 ttcgagccct gtcggcggcg ctttgggccc atagcttagc caggtagagc gcccggctca
     2221 taaccgggcg gccatgggtt cgaaccccat tgggcccatt aatctgctaa tatttcccat
     2281 gctccggtgg tgtagtccgg ccaatcattt cggcctttcg agccgaagac tcgggttcaa
     2341 atcc
//
LOCUS       DAOQVW010000235         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_56461_length_2339_cov_3.292907, whole genome shotgun sequence.
ACCESSION   DAOQVW010000235 DAOQVW010000000
VERSION     DAOQVW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56461_length_2339_cov_3.292907"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PKK85_05685"
     CDS             complement(<1..805)
                     /locus_tag="PKK85_05685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS25543.1"
                     /translation="MVIIKIGVITSAYPEYEDDPHGIFVHRLMKEIAKKGHEVHVIAP
                     HTGGKSKYTMDNVSIEKFHYFYPKGYEKLAGRAGMIDNVKEGWFVKLQVLTFLIFNVW
                     YSLLRFRDVDLVHVQWPIPNGLGALVLKKIFGVPYINTIHGEEVYLAKRYHLQFALRW
                     LVNNSSKTVTNSSATRKACVDAGLDEDKLEVIPFGVDTEFFRPIDVPRTDNYFHILSV
                     GYLIERKGHEYLIKAMKEIVKERDDVRLKIVGSGPLEGKLKSLISELNLD"
     gene            complement(885..>2339)
                     /locus_tag="PKK85_05690"
     CDS             complement(885..>2339)
                     /locus_tag="PKK85_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25544.1"
                     /translation="PGADGICYISIAEKYAIGNFKDAVNGYWGPLFSWLLVPFLLKGF
                     TQLSALKSAWALSLVIGSFNILGMYFLSYRFKVGNTIRTLFVLSLIPVTLYFVYYMLN
                     ADLLMSSFLIFYLNLLFDHKYQNKLNLGFLCGLTGGLAYLSKSFAFPFFLAHFLFFNA
                     IYYFKRDYKNKTILKNLLIGLTVFFLISGVWSLAINEKYGHLTIGTSGTYNYNALGQD
                     VQLNHPMNMFGVVAHPNPTAITPWEDPSYVKLKSWNPFDSTESFQYQLNIILKNIIKT
                     INIIASFSLLVLFILILSIIIYFKSPDERVKWRIFCLMVTILIFILGYIPFFVDTRYF
                     YFLFYLVLLLGVYLISAIKEKYDLKNRMFNVLMVTFFILLIISPTLALIHSTDDKAIY
                     DLSENLKNDFNVHGNIASDSLALQTLFLAYYLNSPFYGATDSYSQDLETGLEKNNIDY
                     YIVWNQTKKINLSHYKEITTTEPNLPRIFKHETA"
BASE COUNT          809 a          431 c          387 g          712 t
ORIGIN      
        1 ggtctaagtt taattccgat attaggctct ttaatttacc ctccagcggc cccgatccca
       61 caatcttcaa cctgacatca tctctttctt tcactatctc cttcatagcc ttgattaaat
      121 attcatggcc ttttctctca atcaaataac ccaccgataa aatgtgaaag taattatcag
      181 ttcgaggaac atcaatcggc ctgaaaaact cggtgtccac cccaaagggt atgacctcta
      241 atttatcctc atccagccct gcatctacac aagctttcct ggtagcagag ctattagtaa
      301 cagtcttact tgaattattc accagccatc gtaaagcaaa ttgtagatga tatcgtttgg
      361 ctagataaac ctcttcccca tgtatggtgt tgatgtaagg aactccaaaa atttttttta
      421 aaacaagggc acccaggccg ttggggatgg gccactgcac atggactagg tcaacatctc
      481 tgaatcttag aagggaatac cagacattga aaataagaaa agtcagaacc tgaagcttta
      541 caaaccatcc ttcctttacg ttatcgatca tccccgcccg tccagctaat ttttcatagc
      601 ctttaggata gaaatagtgg aatttctcta tcgaaacatt atccatggta tatttggatt
      661 ttccaccggt atgtggagca atgacatgaa cttcatgacc ctttttggct atctccttca
      721 taagtcggtg cacaaaaata ccatgaggat catcctcata ttcagggtag gctgacgtta
      781 tgactcctat tttgattata accacctatt ctccttaaat accggttcaa ttagacatct
      841 tgattccata aggttaaata atgagcttct catctagttg atgattatgc ggtttcatgt
      901 ttaaaaattc taggaaggtt tggttctgta gtggttattt ctttataatg gctcaagttt
      961 atctttttag tttgattcca aactatatag tagtcgatat tatttttttc aagccctgtt
     1021 tcaaggtctt gagaataaga gtcagtggca ccataaaaag gggaattaag gtaatacgct
     1081 aaaaaaagag tctgtaatgc gagtgaatca gaggcgatat taccatgaac gttaaaatca
     1141 tttttaagat tttcactaag atcgtagatc gccttatcat cagttgaatg gataagagca
     1201 agagtgggtg aaataattaa taaaatgaag aaagtcacca ttagcacatt aaacatccta
     1261 ttttttagat catatttttc tttgattgca ctgattagat aaacacctaa taatagcact
     1321 aaatagaata aaaagtaaaa atatcgtgtg tcaacaaaaa atggaatata tcctaagata
     1381 aatattagaa tggttaccat caaacagaat attctccatt ttactcgttc atctggtgat
     1441 ttaaaatata ttataatgct taatatgagt ataaatagga caagtagaga aaaagaagct
     1501 attatattaa ttgttttgat tatgtttttt aaaataatat tcagctgata ttggaaagat
     1561 tcggtagaat caaaaggatt ccacgattta agttttacat aggaaggatc ctcccatgga
     1621 gttatagcag tggggttagg gtgtgcaaca acgccaaaca tgttcatagg gtgattcaat
     1681 tggacatctt ggcccagagc attataattg taagtcccag aagtccctat agtcaaatgc
     1741 ccatattttt cgttaattgc taaagaccaa accccactga ttaaaaaaaa taccgtcaaa
     1801 ccaattaaca gattctttaa aattgttttg tttttatagt cccttttaaa gtaataaata
     1861 gcgttaaaaa ataaaaaatg ggccaaaaaa aatggaaaag caaaactttt actaagataa
     1921 gctaaaccac ctgtcaaccc acaaagaaag cccaaattta gcttattttg atatttatgg
     1981 tcaaatagga ggttgagata gaaaataagg aaacttgaca tcaataaatc cgcgtttaac
     2041 atgtaataaa cgaaatacag cgtaacagga atcaaagaca atacaaaaag agttcttatt
     2101 gtatttccaa ctttaaatct gtaacttaaa aaatacatcc ctaagatgtt aaaggatcct
     2161 ataactaaag ataaagccca ggctgatttt aaagccgata gttgtgtgaa acctttcaat
     2221 aggaaaggga ccagcaacca agagaacaaa ggtccccaat acccattcac agcatcttta
     2281 aagtttccta ttgcatactt ctcagcaata ctgatataac atataccatc agcaccagg
//
LOCUS       DAOQVW010000236         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_56911_length_2327_cov_3.661532, whole genome shotgun sequence.
ACCESSION   DAOQVW010000236 DAOQVW010000000
VERSION     DAOQVW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56911_length_2327_cov_3.661532"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKK85_05695"
     CDS             complement(<1..385)
                     /locus_tag="PKK85_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25545.1"
                     /translation="MDYQGQISIEFMMIMATIPMMLLLMATFTCESSELTLAMAAARN
                     GATQGASLDSLAVYPDQAFSNYSQKNQRLLLPSRVQILGIEYQNQGYNPVYQKTKIQI
                     RAYASDPTLKTREDKNCMGERINYYI"
     gene            complement(401..796)
                     /locus_tag="PKK85_05700"
     CDS             complement(401..796)
                     /locus_tag="PKK85_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25546.1"
                     /translation="MVFLIILSGIIGIAEERSKSVYSIQESVEARIIAEKVAQSLEDA
                     YSGGNGHELIVEMSPSINGRDYRVKINSSGVLVDMGSRNCFSTYLAPRVTGPKHTEKN
                     IVLYPSKRYRITHQRDEDGNHFLVIQETI"
     gene            884..1408
                     /locus_tag="PKK85_05705"
     CDS             884..1408
                     /locus_tag="PKK85_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNS25547.1"
                     /translation="MRGEKFDLLIDFNGSRNKWNGQELLGIVELQQVLRKTGKRFFIK
                     ESEFPHTVMVEVENDPLEIYRFLREISCDVISRAMYIESTVETCGNQITQHAVNCIGS
                     KARPGDTFTVKCKVKKRRYINSCHKLMMRIESEIIDGFDLQLDRDQPRWIVHLEFLGE
                     KTGISVLDTEKLVD"
     gene            1442..1939
                     /locus_tag="PKK85_05710"
     CDS             1442..1939
                     /locus_tag="PKK85_05710"
                     /inference="COORDINATES: protein motif:HMM:NF016490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase family protein"
                     /protein_id="HNS25548.1"
                     /translation="MRFRGGAYRFHELAEYLEDAGGMIIQENRLHIIRGSSFIREELH
                     VLMIIPPDEVEHVKSLAQDIKGQVEEDLNLDPQDELDMLHYFYLYHILSQAGGWMNLS
                     LIKDLADQLYHADDPIPDLVYLNYLDGSFPLEYLKKCLDTMCTMQLLEVRDKKGTQEY
                     RVKKD"
     gene            1955..2215
                     /locus_tag="PKK85_05715"
     CDS             1955..2215
                     /locus_tag="PKK85_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00304 family protein"
                     /protein_id="HNS25549.1"
                     /translation="MIRGEYLVTIGAVAIILGILLLIVGTVLLSQGKGEGSAKSTSQV
                     RGGGVIMIGPIPIIFGSDKGSVVLVVILAIILMFLSYLIFYR"
BASE COUNT          635 a          454 c          555 g          683 t
ORIGIN      
        1 gtatgtaata gtttatacgc tcccccatgc agtttttatc ctccctggtt tttaaggtgg
       61 gatcagatgc atatgccctg atctgtatct tggttttctg gtaaaccgga ttgtagccct
      121 gattttggta ttcaatccca aggatttgta cccgggatgg taatagcagg cgctggtttt
      181 tctgcgagta gttggagaaa gcctggtcag ggtaaactgc taagctatcc aggcttgcac
      241 cctgggtggc gccgttgcgt gctgcagcca tggccagggt gagttcactg gattcacaag
      301 taaatgtagc catgagcaac agcatcatgg ggatggtggc cataatcatc ataaattcaa
      361 tggaaatttg tccctggtag tccattcttc caccttcttt ttagattgtt tcctggataa
      421 ccaggaagtg gtttccatct tcatcacgct ggtgggtgat ccggtacctc tttgatgggt
      481 agagaactat atttttctcg gtatgcttgg gaccggtgac tcgtggagct agatatgtac
      541 tgaagcaatt tcgacttccc atatccacca gtactcccga tgaatttatc ttcacccgat
      601 aatcccggcc atttattgag ggtgacattt ccactattaa ttcatgacca ttcccaccgg
      661 aataggcatc ctccagggat tgtgctacct tttctgcaat tattctggct tcaactgatt
      721 cctggataga atatacggat ttagatcttt cttctgcaat tcctattatt cctgacagga
      781 ttattaggaa caccatggat gctaatagaa actccccact catcatactg taaattatat
      841 atgtaatact gtattaataa tatatattac tgtggtgatc tagatgagag gtgaaaagtt
      901 tgatctattg atagatttca atggcagccg gaacaagtgg aatggacagg agctcctggg
      961 aatagtggaa ctgcaacagg tactccgaaa gactgggaag agatttttta taaaagaatc
     1021 agagtttccc cacacggtga tggtggaagt ggagaatgat cccctggaga tctatcgttt
     1081 ccttcgtgaa atttcttgcg atgtaatatc cagggccatg tacatcgaaa gtaccgtgga
     1141 aacctgtggt aatcaaataa cccaacacgc agttaactgc attggatcaa aagctcgccc
     1201 aggagatacc ttcacggtga agtgcaaggt gaagaagcga agatatatta attcctgtca
     1261 taaactaatg atgagaattg aaagtgaaat cattgacggg tttgatctcc agttggaccg
     1321 tgaccagcca cgttggattg ttcaccttga atttttaggt gaaaagaccg gtatatctgt
     1381 cctggacact gaaaaactcg ttgactgatg ggggttagag ttggagtaaa tgtatcggat
     1441 catgagattt aggggcgggg cttacaggtt ccatgaactg gccgaatact tggaagacgc
     1501 cggtggaatg atcattcagg agaataggtt gcacatcatc cgggggagtt cctttatcag
     1561 ggaggagtta catgtcctga tgatcattcc ccccgatgaa gtggaacacg ttaaatccct
     1621 ggcccaggat atcaaaggcc aggtagagga agatctgaac ttggatcccc aggatgaact
     1681 ggatatgtta cattactttt acttgtatca catcctcagc caggctggtg gatggatgaa
     1741 tctatccctg attaaggatc tagccgacca gttataccac gctgatgatc caatcccaga
     1801 tctggtttat ttgaactact tagatggatc attccccctg gaatatctga aaaagtgcct
     1861 ggataccatg tgcacaatgc agttactgga agtccgggac aagaagggaa cccaggagta
     1921 ccgtgtgaaa aaggattaga tgtgattttt tttgatgatc aggggcgaat atttagtaac
     1981 aataggggcc gtggctataa tcctgggaat actcctcttg atagtaggga cagttctcct
     2041 ttcccagggt aaaggtgaag gatcagctaa gagcacttca caagtacgtg ggggcggagt
     2101 gattatgatt ggacccatcc ccatcatttt cggttctgat aaaggcagtg tggtgctggt
     2161 ggtgatcttg gccatcatac tgatgttctt atcctacctt attttctacc ggtgaaatgc
     2221 tagttttttt gaacctttat atctaatttt ttttagatta ggaacaagga atatatttta
     2281 agaaatcata tctaaaaaga aaatgatata agcaaatagt ctgttaa
//
LOCUS       DAOQVW010000237         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_56996_length_2325_cov_2.924670, whole genome shotgun sequence.
ACCESSION   DAOQVW010000237 DAOQVW010000000
VERSION     DAOQVW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56996_length_2325_cov_2.924670"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PKK85_05720"
     CDS             complement(<1..162)
                     /locus_tag="PKK85_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNS25550.1"
                     /translation="MKTRIKELRARKNLTQAQLADEVGVRRETIVFLEKGKYNPSLKL
                     AFLIAKALRS"
     gene            complement(211..786)
                     /locus_tag="PKK85_05725"
     CDS             complement(211..786)
                     /locus_tag="PKK85_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HNS25551.1"
                     /translation="MMEEKFKVFWGLLGFLGILGYILGDPIYYAFFAFFLFFLSPVYS
                     KTTANKDGKVEKKSKMERNYRMYKISIWVGSAILIVTSLYLMATKNMNDYTAMGLLIG
                     MIIYIVSFFAYIGMEKKAGDERLKKIGTLAATWSWYITLIFVGFLLVSMFWAQRMHDP
                     VELMGLVVFILVTTMLVSNTILSRVGDIDLI"
     gene            1119..>2325
                     /locus_tag="PKK85_05730"
     CDS             1119..>2325
                     /locus_tag="PKK85_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HNS25552.1"
                     /translation="MEAKEIFNKLKPLIIVILLFSIVFFIRAEAANLSSLPGDLKAFY
                     QDDDGLPYFSEMDSYYNYRLTADYLDHGYPGDTKMNGKNWDLHSNYPPGRSAEYPPLI
                     IYVTAGAYFLVNLVATVPLTVVSFWMSAFIGSLCVIPAYFMVRRLTNDYGGITAGLLV
                     ALAPAYFNHSFAGFFDTDMFNILLPLLVVWMLVESIRADDLKNKAIFAALSAVSMLIF
                     STAWEGWWYIFYIVVLASLVYLVVSRYLLNQDKKPREDFSNRKEWFLNQPVLLPLAIF
                     VILGSVLMMISMGVLDFFNALVQPVGFTQLQESTQVTSYPNVYVSVAELQIPSISHVL
                     DGVGGILAFTFGIISVFWLFRKIKTPLPENKSKIKKKPRKRKDSQKRRREKELDLKED
                     EKVVPPSVPH"
BASE COUNT          659 a          487 c          447 g          732 t
ORIGIN      
        1 gctcctcaac gcttttgcaa tcaaaaacgc gagtttcaaa gaggggttgt attttccctt
       61 ttccagaaaa actatggtct ctcttcgcac acctacttca tctgccagtt gagcttgggt
      121 gaggttcttc ctggcacgga gttctttgat tcgagttttc atttttagat cgccttgaat
      181 gatatttgct atttcgaatc aaattgatat ttaaatcagg tctatatccc cgacacggct
      241 taaaatcgtg ttagatacca gcatggtggt taccaggata aagacaacca atcccatgag
      301 ttcaacagga tcatgcattc tttgcgccca aaacatggat accagcagaa aacccacgaa
      361 tattagggtt atataccagg accatgttgc agccagtgtt ccgatttttt ttaaacgttc
      421 gtctccggct tttttttcca ttccaatgta ggcaaagaag ctgactatgt aaataatcat
      481 tcctattaat agtcccattg ccgtataatc gttcatgttc ttagtggcca ttaaatacag
      541 tgaagtgact atcagtatcg ctgatcctac ccagatactt attttgtaca ttctataatt
      601 tctctccatc ttggatttct tttccacctt tccatcttta ttggcagtgg ttttactata
      661 cactggggat aaaaagaaca ggaagaatgc aaaaaatgca taatagattg gatcacctaa
      721 aatgtagcct agtatcccca aaaaaccgag taacccccag aatactttga atttttcttc
      781 catcatctcc gcctcattta gatcgtgttg tttatttcca acataatgtt atttaattac
      841 aacattatgt tagattaata taacattact ggtatttata gtattctgct gactccttat
      901 aggaatggaa aagtcacttc ttgttgggat ctttggggga ataattataa atctaacaat
      961 cttacaatat tgctttgaaa ttatcctaag taggataatg caaacttgtt aaaattaaaa
     1021 aaagtaatta agactttaaa cttgcactgg aagtatcaac agcctcattt aaaaggtgat
     1081 ggcagttaca cttccacaca tatctgatgg ggatatttat ggaggccaaa gaaatcttta
     1141 ataaactaaa gccattgatt atagttatat tgcttttttc catcgtattt ttcataagag
     1201 ctgaggcagc gaacttatca tcattaccgg gtgatcttaa agcattttat caggatgatg
     1261 atggtcttcc ttacttcagt gagatggact cttattataa ctaccgttta actgctgatt
     1321 acctggatca cggctatccc ggtgatacta aaatgaatgg taaaaactgg gatctgcact
     1381 ccaactatcc tccagggaga tctgcggaat atcctccact catcatctac gttactgcag
     1441 gggcttattt cttggtgaac ctggtggcta cagtaccctt aactgtggtg agtttctgga
     1501 tgtcggcttt tataggttca ttatgtgtaa taccggccta ctttatggtc aggaggctga
     1561 ccaatgatta tggtggtata accgctgggt tactagttgc attggccccg gcctacttca
     1621 accactcctt cgccggcttc ttcgacaccg acatgttcaa catactccta ccactcctgg
     1681 tggtgtggat gctcgtagaa agtataagag ctgatgattt aaaaaataag gctatattcg
     1741 cagctttatc agctgtttcc atgttaatat tctccacagc ctgggaaggg tggtggtaca
     1801 tattctatat agtggtactt gctagcctgg tctatctggt ggtttcccgc tacctcctga
     1861 accaggataa aaagccgcga gaagatttct ccaaccgtaa ggaatggttt ttaaaccaac
     1921 cagtactcct acccctggcc atcttcgtca ttctgggatc ggttctcatg atgatatcca
     1981 tgggggtact ggactttttc aacgcccttg tccagccagt gggctttacc cagctacagg
     2041 aatccaccca ggtgacttcc tatcccaacg tctatgtatc agtggctgaa cttcaaatac
     2101 catctatatc tcatgtgtta gatggtgtgg gtgggatttt agcctttaca tttggtatta
     2161 tcagtgtttt ctggcttttc cgaaaaataa aaactccgct ccctgagaat aaatcgaaaa
     2221 tcaagaagaa accacgaaag aggaaagaca gtcaaaaaag aagaagggaa aaagagttag
     2281 atttaaagga ggatgaaaag gttgttcctc ccagcgtacc ccacc
//
LOCUS       DAOQVW010000238         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_57011_length_2325_cov_1.892511, whole genome shotgun sequence.
ACCESSION   DAOQVW010000238 DAOQVW010000000
VERSION     DAOQVW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57011_length_2325_cov_1.892511"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..637
                     /locus_tag="PKK85_05735"
     CDS             2..637
                     /locus_tag="PKK85_05735"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016358209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNS25553.1"
                     /translation="MADGWQELVRKIMKEGKEIEDERGSLTKELLNILVHVKKPLGKN
                     ADYYLSNIARLKNIRIPKGYFWSGEKLEIYSQQFLSKDRQGFVYTYGNRLREHFQGVD
                     QIQEAINRLRNCEESRRAISVTWDPVMDTENDEVPCMMLVDLKIRDGKLNVTGLWRSH
                     DIYGAWFPNAVGLANLAQYAAGELGVQVGTLTIHSISAHIYEVNFDEANRF"
     gene            825..1787
                     /gene="mch"
                     /locus_tag="PKK85_05740"
     CDS             825..1787
                     /gene="mch"
                     /locus_tag="PKK85_05740"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HNS25554.1"
                     /translation="MVSVNVEAKKTVDLMIKKADELNIAVDVLSNGSTVIDAGVNVSG
                     SFKAGELYTKVCLGGLADVGISIPGDLSEKFALPSVKIKTDFPSISTLGSQKAGWSVS
                     VGDFFALGSGPARALALKPAETYEEIGYKDDADIAILTLEADQLPGTDVTEAIAKDSG
                     VSPENVYVLVAPTSSLVGSIQISGRVVENGTYKMLEFLHFDVNKVKHAAGIAPIAPVD
                     PDGLKAMGKTNDAVLFGGRTYYYVESEEGDDIKSVAEKLPSSASDGYGKPFFDVFKEA
                     EFDFYKIDKGMFAPAEVVINDLRTGEIFKAGYVNSELLQKSFGL"
     gene            1865..2323
                     /gene="ribC"
                     /locus_tag="PKK85_05745"
     CDS             1865..2323
                     /gene="ribC"
                     /locus_tag="PKK85_05745"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNS25555.1"
                     /translation="MKIGICDTTFARVDMAEYAVNEIKQNLANVEIIRRTVPGVKDLP
                     VACKKLIEEEGCEMIMALGMPGPEAIDKQCAHEASTGLIAAQLMTNTHILEVFVHEDE
                     GLDPKDLKTMAINRATEHAQNLVKMLRGPEQLRKEAGKGMREGRPDVGPV"
BASE COUNT          755 a          445 c          580 g          545 t
ORIGIN      
        1 gatagcagac ggatggcagg aactggttag aaagataatg aaggaaggaa aggagataga
       61 ggatgaaagg ggttctttaa ccaaggaact cctgaatata ttggtccatg ttaaaaagcc
      121 cctgggtaaa aatgcggact attatctgag taatatagcg aggctaaaga atattcggat
      181 accaaaggga tacttctgga gtggggagaa actggagatt tattcccagc agtttttaag
      241 caaagacagg cagggatttg tctacactta tggtaataga ctccgggaac atttccaggg
      301 agttgaccag atacaggaag ctataaacag gcttagaaac tgtgaggaat cccgccgggc
      361 aatttcagta acctgggatc cggtgatgga cacagagaat gatgaggtac cctgtatgat
      421 gctggtcgac ctgaagatcc gtgatggtaa actcaacgtc accggtctct ggcgttcaca
      481 tgacatctat ggggcctggt tccccaacgc cgttggcctt gccaatttag cccagtacgc
      541 tgcaggagaa ctgggcgtgc aggtgggaac ccttaccata cactccatca gcgcccacat
      601 ctatgaagtt aacttcgatg aggcaaacag gttttaattt tctattttaa gccaatttta
      661 aggtaaagca gggtttgatt ttttattttt tatccttaaa aattaaataa aaaaacttaa
      721 aaccagtgga gatttaaaaa gagaaatgga aaatgaattt ttaaattcaa agatgaaatg
      781 atttcaaaag atgaaatata attttagatt tatgaggtgt aagaatggta agtgtcaatg
      841 tggaagcgaa aaaaactgtg gatttaatga ttaaaaaggc agacgaactc aacatagctg
      901 tggatgtctt aagcaacgga tcaaccgtga tagatgcggg tgtaaacgta tcaggaagct
      961 ttaaagcagg ggaactttac accaaggtat gtttaggtgg gctggccgat gtgggaatct
     1021 ccatccctgg agacctatca gagaaattcg ccctgccatc ggtgaagatc aaaacagact
     1081 tcccatccat atccaccctg ggttcccaga aagcaggatg gtcagtaagt gtgggagact
     1141 tctttgcact gggaagtgga ccagcacgtg ccctggcact taaacccgca gaaacctatg
     1201 aagagatagg atacaaggac gatgcagaca tagccatact gaccctggaa gcagaccagc
     1261 tacccggaac cgatgtaaca gaagccatag ctaaagacag cggagtgtca ccagaaaacg
     1321 tctatgtact ggtagcacct acatcatccc tggtaggctc tatacagata tccggaaggg
     1381 tagtggaaaa tggaacctat aagatgctgg aattcctcca cttcgatgtg aataaggtaa
     1441 aacacgcagc cggaatagca cccatcgcac cagtagaccc agacggactt aaagccatgg
     1501 gaaaaaccaa cgacgcagta ctattcggtg gaagaaccta ctactatgtg gaatctgagg
     1561 aaggagacga cataaaatcg gtggctgaaa agctgccatc atcagcctct gatggctatg
     1621 gcaaaccatt cttcgacgtc ttcaaggaag cagagttcga tttctacaag atagacaaag
     1681 gcatgttcgc ccctgctgaa gtggtgataa acgacttacg cactggggaa atattcaaag
     1741 caggatacgt aaacagcgaa ctcctccaga agtcctttgg attataaaac ccttagaacc
     1801 acttgaatta tttaataaat ttttaatttt tttccttgat tttaatgtaa tttggggata
     1861 gaacatgaag ataggtattt gtgatacgac atttgcccgg gtagacatgg cagaatatgc
     1921 cgtaaatgaa attaaacaaa atctggccaa cgtagaaatc ataaggagga ctgtgccggg
     1981 tgttaaggat ttaccagtgg cttgtaagaa attgatagaa gaagagggtt gtgagatgat
     2041 catggcattg gggatgcccg gacccgaggc gatagataaa cagtgcgccc atgaagcatc
     2101 taccggtctc attgctgctc agcttatgac caacacccat atattagagg tattcgtcca
     2161 cgaagatgag gggctggatc ctaaggactt gaaaacaatg gccataaaca gggcaacaga
     2221 acatgcccag aaccttgtta aaatgctcag gggccctgaa caattgcgga aagaagcagg
     2281 taaaggtatg agagagggaa gaccggatgt tggtccggtt taaat
//
LOCUS       DAOQVW010000239         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_57187_length_2320_cov_3.544371, whole genome shotgun sequence.
ACCESSION   DAOQVW010000239 DAOQVW010000000
VERSION     DAOQVW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57187_length_2320_cov_3.544371"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..1340
                     /locus_tag="PKK85_05750"
     CDS             417..1340
                     /locus_tag="PKK85_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNS25556.1"
                     /translation="MRKTIYSTIVILLMGLCMIGAVFAEEPILNATAEKTVNSEEVYL
                     GEAINFNLTVSNPNLDDNFTDVVVTDQISDLLEYLGNSDPSKATYDAATRTVTWNIGN
                     LNLGEVAKLTIFTRAIATGRFDNEFNGTYKVTIVDQPAYDEQVLVGHEKVLTGWDWLP
                     AWGTYYWGSLHQVNTWLDDLRATYPESSYPGAVFWGPFHYTEYFCEICPESEDWYWAW
                     ATIPIYCEDPSRPIYECIHHPATYKKETRYLYAAIDPGIILELPEPVTPETPDVSAAT
                     VTMEKTGAPLLGTILALLLVAGGLFLSKRKP"
     gene            complement(1471..>2320)
                     /locus_tag="PKK85_05755"
     CDS             complement(1471..>2320)
                     /locus_tag="PKK85_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNS25557.1"
                     /translation="RYQMRGLEVEPEEVLVTNGSQQGLDLVGKIFLNQKDPVLVERPT
                     YLFVLQSFGLYEPIFHSLPLGQEGVDVLDLQEVLNRFRPKLFYAVPNFQNPTGTSYSL
                     ECRKEVARVLKGSDTVLVEDDPYGEIRFLGEDHPPVKAFLGESLFLGSFSRVVSPGIR
                     LGWVTAPSEVMDKLVTAKQASDLHTSLLSQMIIHCYLSDYDVEAHLKRIRSMYKRKRD
                     LMVSLIKDEFPEDVKCTEPGGECSCGLPSRRVCPPWSYLKEPLRRRWPLYQARPSSLK
                     ILEITP"
BASE COUNT          661 a          558 c          521 g          580 t
ORIGIN      
        1 cctgccttta accaatttta taatccataa agataccagt tccaggtgga tttccagtgt
       61 aaatattgtg aattaacact aatttttctc tgccatggca aattataaat atgttctgga
      121 ataaaaacaa agaacagaaa aagaaaaagg gggggataac aaaagatgag tatccataaa
      181 gcggaagaag tgaccaccca taatgtgggg agcccagtat ggttcaggcc accttattgt
      241 ggtgttcgcg gtattcgtta tggtgatgtg gtgatagatt tcgccttaat acattattta
      301 aggagtagat actatccatt ggcgggggaa tcaccttagc ttaagacacc acacttataa
      361 cgataagtta ttggattatt tttataaaac gaaatatatc acttgggggg gatatattga
      421 gaaagactat ctactctacc atagtgatcc tgttaatggg attatgcatg ataggcgctg
      481 tttttgcaga agaaccaatt ctgaatgcta cggcagaaaa gacggtgaac agtgaagaag
      541 tttatctggg tgaagctata aattttaacc ttacagttag caatccaaac ctggatgaca
      601 acttcaccga cgtggtggtt acagatcaaa tctcggactt gttagagtac cttggaaaca
      661 gtgatccttc gaaagcaaca tatgatgcag ccacaaggac cgtaacctgg aatattggta
      721 atctaaacct tggggaagtg gcaaaactga ccatttttac cagagccata gccactggta
      781 gatttgacaa cgaattcaac ggaacctaca aggtaaccat tgtggatcaa cccgcctacg
      841 atgaacaagt gctggtaggc catgaaaaag ttctaactgg ctgggactgg ctccctgcct
      901 ggggaacata ctactggggc agccttcatc aagtaaacac gtggcttgat gatctgcgtg
      961 ccacctaccc agaatcttcc taccccggtg ctgtgttctg gggtccattc cactacacag
     1021 aatacttctg tgagatatgc ccagaatccg aggattggta ctgggcctgg gccaccatcc
     1081 ccatctactg tgaagatccc agccgaccaa tctacgaatg catacaccac ccagccacct
     1141 acaaaaaaga aaccagatat ctctatgctg ccattgaccc gggaattatt ctggaactac
     1201 cagaacctgt aactcccgaa acacctgacg tatctgctgc tacagtgacc atggaaaaaa
     1261 caggagcacc actactggga accatcctgg ccttactact ggtagccggg ggactgttcc
     1321 tatcaaaaag aaaaccatag ggggaactaa taaaccccct tttttatatt ttttttatga
     1381 acaactaatt ttcaagttct cctagagacc tggccaaccg atttataccc tcttctatct
     1441 cttccctgct gctgttggaa aaattcatcc tcaaggtgtt atctcgagga ttttcagaga
     1501 agaaggccgg gcctggtaca aaggccacct tctcctcaag ggctctttta aatagctcca
     1561 gggaggacac accctccggg agggtaaccc acaggaacat tccccccctg gttcggtgca
     1621 cttcacatcc tccgggaact catcctttat taaagagacc atgaggtccc gctttctttt
     1681 gtacatgctg cgtattctct ttaaatgagc ctccacatca taatctgata ggtaacagtg
     1741 gatgatcatc tgggacagta gactggtgtg cagatcagag gcctgcttgg cggtgaccag
     1801 cttatccata acttcggagg gggcggttac ccatcccaga cggatgcccg gggaaactac
     1861 cctggaaaaa gaccctaaaa ataaggattc acctagaaag gccttaactg ggggatgatc
     1921 ctcgcctagg aagcgtatct caccgtaggg atcatcctcc accaaaacag tatcactgcc
     1981 ctttaaaacc ctggcaacct ccttcctaca ctccagggaa taacttgtgc cggtggggtt
     2041 ctggaagtta ggaactgcgt agaagagttt gggacgaaat ctattcagaa cctcctgcaa
     2101 gtctagaaca tccactccct cttgtcctag gggtagggaa tggaatattg gctcgtagag
     2161 cccaaaggac tggaggacga ataggtaggt gggtctttcc accaggaccg ggtccttctg
     2221 gttgaggaat atcttcccca ccagatccag gccctgctgg gaaccattgg tcactagaac
     2281 ctcctccggc tccacttcta ggcccctcat ctggtagcgc
//
LOCUS       DAOQVW010000240         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_57871_length_2302_cov_3.922118, whole genome shotgun sequence.
ACCESSION   DAOQVW010000240 DAOQVW010000000
VERSION     DAOQVW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57871_length_2302_cov_3.922118"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKK85_05760"
     CDS             <1..581
                     /locus_tag="PKK85_05760"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS25558.1"
                     /translation="GRKRDQDSLVSLEEALADGNNDVRSNAALALGEIGDPQPVPILI
                     KTLTDDEWHVQVHAAEALGKIQDARALEALINALDNDKIRNQAAMALGMIGDPRAVLH
                     LQLALDDEDFSFRSAAIEALGMIGDPLALESLMEALQDGDVSVRRHAASALGKIGDQR
                     AVPALKKALEDERWYVRLQVEEALQDIQAVQK"
     gene            complement(602..1057)
                     /locus_tag="PKK85_05765"
     CDS             complement(602..1057)
                     /locus_tag="PKK85_05765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNS25559.1"
                     /translation="MHECPYCKKLEAYNFGDYLGKTEHWIIFLAPNQSNLGTCVIALT
                     RHHPTLTGLSNEEWEEFIELVEKMENAVKKAFQATLVNWGCLMNSFYLENNPDPHLHW
                     HFIPRYQNSVEFAGQLFEDPHFGYMRPRPLKKISHDVREKILNKIKEYM"
     gene            complement(1076..1819)
                     /locus_tag="PKK85_05770"
     CDS             complement(1076..1819)
                     /locus_tag="PKK85_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HNS25560.1"
                     /translation="MLWDLVLSGLWGFLGGFALIIGAIMGFYFRIPPRLVAGIMAFGS
                     GVLLSAISFELLDEAYYLGGFLDVAAGFLVGAIIFTLTNLYLARKGAKHRKRSQRPQD
                     FEGSGVAIAAGSVIDGIPESIAIGLTMIGGVGVSTATLVAVFLSNIPEGLSSTVGMKS
                     DGWKPTHVFGLWLVIAISTALASIIGYSVFQYLPDAFTSVALAIAAGGILAMLVDTMI
                     PEAFSQSHDLSGLITVVGFTVSFILSKLY"
     gene            1915..>2302
                     /locus_tag="PKK85_05775"
     CDS             1915..>2302
                     /locus_tag="PKK85_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS25561.1"
                     /translation="MDRSLQRVTLFLVSLAAFLIPFMGSSLSLALPVIQKEMAVNVII
                     LGWIPTSFTLANAVTVLPFGRLSDIHGRKRIFSYGVLIYTISSLFAAFSNSGEILVAF
                     SFLQGLGCAMIFATMVALLSSVFPMDR"
BASE COUNT          567 a          594 c          593 g          548 t
ORIGIN      
        1 tggggagaaa acgggaccaa gatagtctgg tgtcgttaga ggaggctctg gctgatggta
       61 acaatgacgt gcgatccaac gccgccctag ctctgggaga aattggagac ccccaacctg
      121 tacctatact cattaaaacc cttaccgatg acgagtggca tgtgcaggta cacgccgcgg
      181 aggccctggg aaaaatacaa gatgcccggg ccctggaagc gttgataaat gcattggata
      241 atgataagat aagaaaccag gcagccatgg ccctgggtat gattggtgat ccccgggcgg
      301 ttttacacct gcaactagcc ctggatgatg aggatttcag cttccgcagc gctgccatag
      361 aagctttggg tatgattggt gatcccctgg ccctggaatc ccttatggag gcccttcagg
      421 atggggatgt cagtgtccgc cggcatgctg ccagtgccct gggaaaaatt ggagaccaac
      481 gggcggtgcc cgccctgaaa aaagccctgg aagacgagcg atggtacgtt cgcctgcagg
      541 tggaagaggc attgcaggat atacaagctg tccagaaata atcagggcct tcacacaaaa
      601 ttcacatgta ttccttaatt ttattcaata tcttttcccg aacatcatgg gagatttttt
      661 tcaggggacg aggacgcatg tacccaaagt gggggtcctc gaagagttgt ccggcgaact
      721 ccaccgagtt ctggtagcgg ggtatgaagt gccagtggag atggggatcc ggattgttct
      781 ccaggtagaa cgagttcatg agacatcccc agttgactag ggtggcctgg aaggccttct
      841 taaccgcatt ttccatcttt tccaccagtt caatgaactc ttcccattct tcattgctta
      901 acccggtcag ggtgggatgg tgccgggtga gggctatcac acatgtgccc aggttgctct
      961 ggttgggtgc caggaaaatt atccagtgct cagtctttcc caggtaatcc ccgaagttgt
     1021 aagcttccag cttcttgcag taaggacatt cgtgcataat aaatcgccta tttttttagt
     1081 acaatttgga cagtatgaag gatacggtga atcccaccac ggttatgagg cccgacaggt
     1141 catggctctg gctgaaggcc tctggtatca tggtgtccac cagcatggcc aggattccac
     1201 cggctgctat ggccagggca acggaggtga atgcatctgg aagatactgg aacacggagt
     1261 atcctatgat agaagccagg gctgtggaaa tggcgatgac cagccacaaa ccgaaaacat
     1321 gggtgggctt ccaaccgtca cttttcatac caaccgtgct ggacaggccc tcaggtatat
     1381 tggacaaaaa cacggccacc agggtagcgg tgctcactcc cacccccccg atcatggtta
     1441 aaccaatggc tatggactcc gggatgccgt cgataaccga acctgctgct atagccaccc
     1501 cactgccctc gaaatcctgg ggccgctggg accttttccg gtgcttagcc cccttcctgg
     1561 ccagatacag gttagttagg gtgaagatga tggcccctac caggaaacca gccgctacat
     1621 ctaggaagcc tcccaggtaa taggcctcat ccaggagttc gaaggaaatt gctgataaga
     1681 gcaccccact accaaaggcc atgatacccg ccactagccg gggtggaatc ctaaaataga
     1741 agcccataat ggcccctata atcagtgcaa atcctcccag aaatccccac agaccagaga
     1801 gcaccaagtc ccataacata cctagaatca ccacatgtat atttatgtat gaggatcagg
     1861 gcatataatt aattagggca aaaaaaagat tggaggggtg atgatttttt gattgtggac
     1921 cggtcactgc agagggtaac cctatttctg gtatccctag ccgccttcct aatacccttc
     1981 atgggatcct cccttagttt ggccttaccc gtcatccaga aggagatggc agtcaacgtg
     2041 ataatcctgg gatggatacc cacctccttc acactggcta acgctgtcac ggtccttccc
     2101 ttcggccgtt tgtccgatat tcacggccga aaaagaattt tctcctatgg agttctaata
     2161 tacactatat cctctctttt cgctgctttt tccaattccg gtgagatcct agtggccttc
     2221 agcttcctgc agggcctggg atgtgccatg atcttcgcca ccatggtggc gcttttaagc
     2281 tctgtttttc caatggatcg ta
//
LOCUS       DAOQVW010000241         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_58111_length_2296_cov_3.184293, whole genome shotgun sequence.
ACCESSION   DAOQVW010000241 DAOQVW010000000
VERSION     DAOQVW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58111_length_2296_cov_3.184293"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKK85_05780"
     CDS             <1..254
                     /locus_tag="PKK85_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824607.1"
                     /note="functions to produce tRNACys in organisms that lack
                     cysteinyl tRNA-synthetases; SepRS; uses modified amino
                     acid; subclass IIc aaRS; attaches O-phosphoserine to
                     tRNACys; the product of Mj1678 converts the Sep-tRNACys to
                     Cys-tRNACys; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HNS25562.1"
                     /translation="DALKNGVNTGISYMDGVAAQAAYQAEEMVVSGSESMKIRTTIAK
                     APSDINLILDKVALRYITSNNLEIDIRGPIFCTITSQLL"
     gene            283..957
                     /locus_tag="PKK85_05785"
     CDS             283..957
                     /locus_tag="PKK85_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNS25563.1"
                     /translation="MCSFNLIQLLFNIQNILALQVVAEDCFENLERVAGADISFSQGT
                     KAVAAAVVMDLDEQAVVEKQTTPVILEFPYVPGFLGFREATATVSILKKVKTDFDVLM
                     INGHGILHPRGFGLASQVGVLMDVPTIGVAKRLIKGRYKHQASQLHHKKETPHLILDS
                     KVVVGAFFRRNYVSVGHKISLYTALEVVKRSSIYHTPEPLRQAHMLATEKFKEEIAAC
                     NSIKPN"
     gene            966..1343
                     /locus_tag="PKK85_05790"
     CDS             966..1343
                     /locus_tag="PKK85_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP-dependent riboflavin kinase"
                     /protein_id="HNS25564.1"
                     /translation="MKIQGEIISGDNKGQYFMSLDIYKIQFMGILGFEPFPGTLNLKI
                     DAGGAEKIGELMEKMDSVKGRGKFGDVKLIKARLDRDFEGALVFPVKTHHEPDVLEFI
                     SPLNLRKSLQLQDGDHVILEIFD"
     gene            1385..2062
                     /gene="ribB"
                     /locus_tag="PKK85_05795"
     CDS             1385..2062
                     /gene="ribB"
                     /locus_tag="PKK85_05795"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNS25565.1"
                     /translation="MIKKVLDSYKKGEIVLIFDDDNRERETDMIVAGEFITPAQIARM
                     RNDAGGLICVPLSAAVADELRVPFMTDIMQAASSEYPVLTELSPTDIPYDEKSAFSIT
                     VNHRKTFTGITDNDRTLTINELAKMAKNNMGSQFGKYFRSPGHVTLLRAAEGLLLKRK
                     GHTEMSVALAEMCGLTQVAVCCEMMDDATGDSLPTDEVEKYAEKEGLVFLSGAEVIES
                     YQEFKKD"
     gene            complement(2059..>2296)
                     /locus_tag="PKK85_05800"
     CDS             complement(2059..>2296)
                     /locus_tag="PKK85_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25566.1"
                     /translation="ASSVPKVKMEVRGGTPELEIKLDDFCLNAMKTVEKYLNPDELVK
                     LAEFPSFQGYMTHQDILEGFKIGPKEDENRKTGI"
BASE COUNT          699 a          443 c          523 g          631 t
ORIGIN      
        1 aggacgccct taaaaatgga gttaacaccg gaataagtta catggatgga gtggcagccc
       61 aagctgcata ccaggcggag gaaatggttg tgagtggatc ggagagtatg aagatacgca
      121 ccaccattgc caaggccccc tctgatatta acctcattct ggataaagtg gctctgcgct
      181 acataaccag taacaacctg gaaattgaca ttagaggacc tatcttctgc accataacaa
      241 gtcaactact atgataaatt ctactatacc aaattgactc ttatgtgttc ctttaacctc
      301 attcaactgt tattcaacat acaaaacatc ctggccctcc aggtagtagc cgaggattgc
      361 ttcgagaatt tggaaagagt tgcaggggca gatatttcct tttcccaggg cactaaggca
      421 gtagctgcgg cggtggtaat ggatctggat gaacaggcgg tggtggagaa acaaaccacc
      481 ccggtgatcc tggaatttcc ttatgttcca ggttttttag gtttcagaga agcaacagct
      541 actgtatcta ttttgaaaaa ggttaaaacg gattttgatg ttctgatgat taacggtcat
      601 ggaatattgc atccccgtgg ttttgggctg gcttcccagg tgggggtgct gatggatgtt
      661 cccaccatag gcgttgcaaa aaggttaatt aaaggtagat ataaacacca agccagtcaa
      721 cttcaccata aaaaagaaac accccacctt atacttgact ctaaagtagt cgtgggggcc
      781 ttttttagaa ggaattatgt gagtgttggt cataaaatat cgttatacac ggctttagaa
      841 gttgtgaaga ggagcagtat ttatcataca cctgagccgt tacgacaagc ccatatgtta
      901 gctactgaaa agtttaaaga agaaatagct gcatgtaatt caataaaacc gaattaagtg
      961 atttgatgaa aattcaggga gaaataattt ccggtgataa taaaggtcaa tatttcatgt
     1021 ctttagacat ctataaaatt caatttatgg gaatacttgg atttgaaccc tttcctggta
     1081 cgttgaacct taagattgat gcaggtggtg ccgaaaaaat tggtgaactc atggaaaaga
     1141 tggatagtgt caaaggaagg ggcaaatttg gagatgtaaa gttaataaaa gcaaggctag
     1201 atagggattt tgagggtgcc ctggtcttcc cagtcaaaac tcatcatgaa cccgatgttc
     1261 tggaattcat ttccccactg aatctgcgta agtccctgca actacaggat ggggatcatg
     1321 tgattctgga aatatttgat taataacaat tatcaatgaa attcttaagc cagagagtga
     1381 tttaatgata aaaaaagttc tagatagtta taaaaaagga gagatagttt taatctttga
     1441 tgatgataac cgggaaaggg aaacagacat gatagtggct ggtgaattca ttactcccgc
     1501 ccaaatcgcc agaatgagga atgatgccgg ggggctcatc tgcgtcccat tgtcagcagc
     1561 agtggccgat gaactgagag ttccctttat gaccgatata atgcaagctg ccagcagtga
     1621 gtatccagtc ctcacagagc tatcccccac tgacataccc tacgacgaaa aatcagcctt
     1681 ttccataact gtgaatcacc gaaaaacctt caccggaata actgacaacg accgtaccct
     1741 gaccattaac gagttggcca aaatggcaaa aaacaatatg ggaagtcagt ttggaaagta
     1801 tttcagatcc cctggacacg ttactctact ccgagctgca gaggggcttt tattgaaaag
     1861 aaagggacat actgagatga gtgtggccct ggcagagatg tgtggcctta cccaggtagc
     1921 agtatgttgc gagatgatgg atgatgctac tggagattcc ttacccaccg atgaggtgga
     1981 aaagtatgca gaaaaggagg ggctggtctt tttaagtggt gctgaggtta ttgaatccta
     2041 ccaggagttt aaaaaagatt aaattcctgt ttttctattt tcatcctctt tagggcctat
     2101 tttaaatcct tctagaatat cttgatgggt catgtatccc tggaatgagg gaaattccgc
     2161 taatttcacc agttcatccg ggtttaaata tttttcaaca gttttcatgg cattcaggca
     2221 gaaatcatcc aattttattt ctaactctgg agttcctccc cggacttcca tctttacttt
     2281 aggcacggaa cttgca
//
LOCUS       DAOQVW010000242         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_58713_length_2281_cov_2.350854, whole genome shotgun sequence.
ACCESSION   DAOQVW010000242 DAOQVW010000000
VERSION     DAOQVW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58713_length_2281_cov_2.350854"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKK85_05805"
     CDS             <1..390
                     /locus_tag="PKK85_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01210 family radical SAM protein"
                     /protein_id="HNS25567.1"
                     /translation="ISDAIQSAEYAEQSGVDRVSFCPSTIHKGTLMEMLWRKGSYQPP
                     WIWSVIEIIRRVRSLVKIPVIMDTAGFGTRRGPYNCKKCNSALKDMIIQSNIHQTIPE
                     DFECKCRSKWLAEVEYSGITHSASKLS"
     gene            complement(412..1083)
                     /locus_tag="PKK85_05810"
     CDS             complement(412..1083)
                     /locus_tag="PKK85_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS25568.1"
                     /translation="MNEIIVLEKVRKEYHVGEETVNALRGLDLVIKKGSFIAIMGPSG
                     SGKSTLLHLAGILDVPTTGTVSLEGKDIKEYSSKEQAQLRRHKIGFIFQRYNLMPQLT
                     VLENVLLPMVKPDPEKAKAILDKLGLSDKYDRFQGQLSGGEQQRVVIARALANDPAII
                     LADEPTGELDTENTRIIMNILRELNQKEGINIVVVTHNPIAAEYAEKVIKMQDGVIIG
                     ENSQN"
     gene            complement(1076..2158)
                     /locus_tag="PKK85_05815"
     CDS             complement(1076..2158)
                     /locus_tag="PKK85_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS25569.1"
                     /translation="MGIYRLSLKNIKRRKLRSILTMLGIIIGVATLVLLIGAGTGMKA
                     YTKDQIQSMAGDIVIYNSSGGMYASGEYYLNSATVSKIKNLSQIYNVREDTQFNSNIK
                     NSPVVVVGLSDWSQIKVNGTTGVVIDKAMADKFGYKIGSTITIKDQEFTITGTTKQMS
                     MGMGLVFLDIDKALPLNDNKVSSVTASTKGDPESVKKDVESQVPGTMALTQSDFTKQI
                     DDMMNGIMLFIGAIASIGLIVGVISIVNIMLVNVTERTREIGVLKAIGFTNHEVLGSI
                     LVEAGFLGFIGAVIGLILAAVLLQLAIIFFGPQLGMEDINLLYMLPPWLIVAVIGGAT
                     LLSMLAGLYPAWKASRLNVVEALRHE"
BASE COUNT          588 a          548 c          464 g          681 t
ORIGIN      
        1 attagtgatg ccatccaatc ggcagagtat gcagaacaat caggagtgga ccgtgtttca
       61 ttctgtcctt ctaccataca caagggtact ttaatggaga tgctctggag gaagggttct
      121 tatcagccac cctggatctg gagtgtcatt gaaataataa gaagagtccg gagtctagtc
      181 aagattccgg tgatcatgga caccgccgga tttgggacac gtagggggcc ctacaactgt
      241 aaaaagtgta actctgccct gaaggatatg atcatccagt ccaacataca tcaaaccatt
      301 cctgaggatt ttgaatgcaa atgcagaagt aaatggctgg cggaagtaga atactccgga
      361 ataacccatt ctgccagcaa attgtcataa aaagaatcta cttctgccaa actagttctg
      421 gctattttct cctattatga caccatcttg catttttata accttttccg catattctgc
      481 agcaataggg ttatgggtca ccactacaat gtttattccc tctttctgat taagttcgcg
      541 gagtatgttc attattatgc gggtgttctc ggtgtccagt tctccggtgg gttcatcagc
      601 caggatgatg gcaggatcat tggccagagc acgagctatg accacccttt gttgttctcc
      661 gccggaaagc tgtccttgaa atcgatcata tttgtcagac aggcccagtt tatctagaat
      721 ggccttggct ttttctggat caggtttaac cataggtaat agaacgtttt ccagtacagt
      781 gagttggggc atcaggttgt agcgttgaaa gatgaacccg attttatggc gacgtaactg
      841 tgcctgttcc ttactggaat attctttaat atctttacct tccagggata ctgttccagt
      901 ggtaggcaca tccagtatcc cggccaggtg taacagggtg gatttcccgg aaccagaggg
      961 gcccattatg gctatgaagg aacccttctt tatcaccaga tccagtcccc gaagagcatt
     1021 tacggtctct tcacccacat gatattcctt acgaactttt tccaggacta ttatttcatt
     1081 catgacgcaa cgcctccacc acgttcaacc tggacgcctt ccacgccgga tacaagccgg
     1141 ccaacatact gaggagtgtg gccccaccga tgacagccac aatcaaccag ggagggagca
     1201 tgtaaagcaa attaatgtct tccataccca gctgcggtcc gaagaaaatt atggccaatt
     1261 gaaggagtac tgccgccagt atcaaaccaa ttacagctcc gataaaacct aaaaagccgg
     1321 cctccaccag gatgctaccc agcacttcgt ggttggtgaa tcctatggcc tttaaaacac
     1381 ctatctcgcg ggttctctcg gttacattca ccagcataat gttcacgata ctaataactc
     1441 ccacaattag tccgatgctg gcaatggctc cgatgaatag cattatgccg ttcatcatat
     1501 catcgatctg ttttgtgaaa tcggactgag tgagggccat ggtccctgga acctgacttt
     1561 ccacgtcctt tttaacagat tctggatccc ccttggtgct ggcagtgaca ctcgacactt
     1621 tgttatcatt tagtggcaat gccttatcaa tgtccaggaa cacaagaccc attcccatgg
     1681 acatttgctt ggtagtgccg gttatcgtga actcctgatc tttaattgta attgtgctac
     1741 ctattttgta accaaactta tcagccattg ctttatcaat taccactcct gtggttccgt
     1801 tcaccttaat ctgactccaa tccgaaagtc ccaccaccac tacaggagag tttttaatat
     1861 tcgaattaaa ttgagtatct tcccgtacgt tataaatttg tgataagttt ttaattttgg
     1921 aaacggtggc tgaatttaga taatattctc cagacgcgta catgcccccg gaactattgt
     1981 atattactat atctcctgcc atggactgaa tttggtcttt ggtgtaggct ttcattcccg
     2041 taccggcgcc tatgagaagt accagggtgg ccaccccgat tattatgccc agcatggtca
     2101 ggatgcttct gagctttctt cttttaatgt tcttcaatga taatctgtaa attcccattt
     2161 tgtaacccct ttatgattac ttatatgaaa tttattacta tttattataa gcttcctcaa
     2221 tttacatctt cctcgatttc aaacacttcc tcaacatgat ctttcttcag agcccttgtt
     2281 a
//
LOCUS       DAOQVW010000243         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_59451_length_2263_cov_3.375000, whole genome shotgun sequence.
ACCESSION   DAOQVW010000243 DAOQVW010000000
VERSION     DAOQVW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59451_length_2263_cov_3.375000"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PKK85_05820"
     CDS             complement(<1..490)
                     /locus_tag="PKK85_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNS25570.1"
                     /translation="MKMEKITTNTLTQALEGVNYLPDENLVIILFLALQMKKPILVEG
                     PPGTGKTELAKAASRALEREFFRVQCYEGITFEQIVGEWNYQKQLLQLEISRQKSGEE
                     DIFQDDFFIKRPLLQAFINQEPSVILIDEIDKADEEVESFLLQALGEKEITVNDLGTF
                     QLA"
     gene            629..772
                     /locus_tag="PKK85_05825"
     CDS             629..772
                     /locus_tag="PKK85_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25571.1"
                     /translation="MNGSDTPPGPLSGGDQRNLYTSTPFITCLERIPQHWALIGGEQL
                     LEL"
     gene            785..1099
                     /locus_tag="PKK85_05830"
     CDS             785..1099
                     /locus_tag="PKK85_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25572.1"
                     /translation="MHPAPSPWSLASTPREKLSGWAVSRWCSYHPKWIPVQRPFRGVK
                     KILPNFNHYQTTSTLSFTEPVATSGYRTLVYEVPDLASNQDAQEKTKKLIGAIFSTLI
                     LI"
     gene            complement(1096..1488)
                     /locus_tag="PKK85_05835"
     CDS             complement(1096..1488)
                     /locus_tag="PKK85_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25573.1"
                     /translation="MFEEETRVHDLFITHNGPGDEEYISFIQRLLEAPDFEWKDHGVP
                     GQNQEEEIEEQIQASQVVVVLSGLYNRYPEVKKQVKIARDMEKPMLLIRPYGLEEVPV
                     ELEEWASGVVGWNRVCIVEEIEEILGLY"
     gene            complement(1494..1706)
                     /locus_tag="PKK85_05840"
     CDS             complement(1494..1706)
                     /locus_tag="PKK85_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25574.1"
                     /translation="MSCYLRYMKDVLEEADLHPQDRKERKEVDLAIRRIVGKSEKDQC
                     NMVWKEVKLWLAADQKKALLVENLKD"
     gene            1796..>2263
                     /locus_tag="PKK85_05845"
     CDS             1796..>2263
                     /locus_tag="PKK85_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNS25575.1"
                     /translation="MGSYTRSTLGLILMKTFQIALCQMKVGLNKAQNIKKSLEFIEEA
                     GKKADLVVLPEMFNCPYQTDQFPLYAERASESPTLKAVSQAARDSEVYLVAGSIPERD
                     QESIYNTSFIYNPSGELLARHRKLHLFDIDIPGQIQFKESDTLQPGGQVTVVDT"
BASE COUNT          548 a          646 c          498 g          571 t
ORIGIN      
        1 tggccagttg gaaggtgccc aggtcgttga cggtgatctc cttctccccc agggcctgca
       61 ggaggaagct ctccacctcc tcatcggcct tatcaatctc atcaataagt atcaccgagg
      121 gctcctggtt gatgaaggct tggagcaggg gacgtttaat gaagaagtca tcctggaaga
      181 tatcctcctc cccactcttc tggcgggaga tctccaactg caaaagctgc ttctggtagt
      241 tccactcccc tacgatctgt tcaaaggtga tgccctcgta gcactgcacc cggaaaaatt
      301 cccgttccag ggcccgggag gcagccttgg ccagttcagt tttaccagtg cctggagggc
      361 cctccaccag gatgggcttt ttcatctgca gcgccagaaa gaggatgatc accaggttct
      421 catctggcag gtaattaaca ccctccaggg cttgggtcag ggtgttggtt gttatcttct
      481 ccatcttcat atgatcaact actatgggat gaactactac taatgtgttg gatatccaca
      541 tattatgctc tgcttgatcc tggtctgggt tgatccatca gtctcctcca tatagaataa
      601 atccctccag atacctagga taatagaagt gaatggctct gatacccctc ctggtcctct
      661 tagtggtggg gaccagcgta acctctacac ttctaccccc ttcatcacgt gtctggaaag
      721 gatacctcag cactgggctc tgattggagg ggaacagtta ctagaactgt gatgggcaat
      781 tccagtgcat ccagcaccat cgccgtggtc actggcatcc accccccgtg agaaactctc
      841 cggctgggca gtctccaggt ggtgtagtta tcaccccaaa tggattccag ttcagcgacc
      901 cttccggggg gtaaaaaaaa ttctgcccaa tttcaaccac taccagacca catccacact
      961 gagtttcaca gaaccagtag ccacctctgg gtaccgtacc ctggtctacg aggtacctga
     1021 tttggccagt aaccaggacg cccaggagaa gactaaaaaa cttatagggg ccatcttcag
     1081 caccctaatc ctgatctaat atagccccaa aatttcttca atctcttcca cgatacacac
     1141 ccggttccag cccaccactc cactggccca ctcttccagc tccacaggta cctcttccag
     1201 tccatagggc cggatgagaa gcatgggttt ctccatatcc cgggctatct tcacctgttt
     1261 tttcacctcg gggtagcggt tgtagaggcc agagagcacc acaacaacct gggaggcttg
     1321 gatctgctct tcaatctctt cttcctggtt ttggcctgga acaccatgat ctttccactc
     1381 aaaatcaggg gcctccagga gtctctggat gaagcttatg tactcctcat ctccaggtcc
     1441 attgtgggtg atgaacaagt catgtaccct tgtttcctct tcaaacatgt gatctaatcc
     1501 tttaaattct ccaccagcaa ggcctttttt tggtcagcag ccaaccagag cttaacctcc
     1561 ttccacacca tgttacactg gtctttttca cttttcccca ctatccgccg gatggccagg
     1621 tcaacctctt ttcgttcctt ccggtcctgg ggatgaagat cagcctcctc caggacatcc
     1681 ttcatgtagc gtaagtagca actcatgaaa acacctcgct tatcagttta gataattata
     1741 ttatggccac aattaataat catgactagt gggacccaat gaggaccttc cccacttggg
     1801 cagttacacc agatccaccc tgggtttgat tcttatgaaa acattccaga tagcactgtg
     1861 ccagatgaaa gttgggctaa acaaggccca gaatattaaa aaatcccttg aattcataga
     1921 agaagctgga aaaaaggctg atctggtggt tttacccgag atgttcaact gcccctacca
     1981 gaccgaccag ttccccttat acgctgaaag ggcaagtgaa agccccacct taaaggctgt
     2041 ttcccaggcg gcacgggaca gtgaggtcta cctggttgct ggatccatac ctgaaaggga
     2101 ccaggaatct atctacaaca ccagcttcat ctacaacccc tccggggagc tgttagctcg
     2161 gcaccgtaag ctgcaccttt ttgatatcga tattcccggc caaatccagt ttaaagaatc
     2221 agacaccctc caacccgggg gccaggtcac agtggtggac acg
//
LOCUS       DAOQVW010000244         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_59909_length_2253_cov_3.051865, whole genome shotgun sequence.
ACCESSION   DAOQVW010000244 DAOQVW010000000
VERSION     DAOQVW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59909_length_2253_cov_3.051865"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2044)
                     /locus_tag="PKK85_05850"
     CDS             complement(<1..2044)
                     /locus_tag="PKK85_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNS25576.1"
                     /translation="MIILRKKNKIVEIFPIGSSKDAINQRRKPLFYAYLKLRRIKGQL
                     KIHRFVVQKDKEMILPPSEAVKILRKQNVFLVGDEPEVEELMESLHIKYRKTSICRHC
                     TFTGHITLLEPEKSFRYHQDNICLICAEEEIKRELKNHSFDLKIFPTFQRMLNRTGDL
                     ETVLKTFNPRFNPLKERNLTLYDQMAGGGDDKDFKMDMEELDVPPNFKIILKKQGTHL
                     LPVQVMAVNVGLLAGENLLVVSATASGKTLIGELAGIPRALKGGKFLYLSPLVALANQ
                     KYRDFQKRYGKLDLKVSIKVGMSRIKAREELSLPKGKVEDSDIVVGTYEGLDFLLRAG
                     RASELSNLSTVVVDEIHMLGDAERGPRLKGLIQRLRFLFPNLQIIGLSATVGNNQEIA
                     NEFGMKLVEYSKRPVPLERHLIFARSEEDKNHWMTQLTRSEFQKRSQKGFHGQSIIFT
                     NSRRKTHSIADYLVKRGIKAAAYHAGLSYSKKNRIEKTFGEQKISVVVTTAALAAGVD
                     FPASQVIFESFNMGREMLTPNEFSQMMGRAGRPSYHDQGKIYLIPEVGRRYGDDTEEN
                     MAVTLLDSDVEPIHVQYSEAGLVEQVLADVCARGPSEIHEISKEFEKDEIPMEIESAW
                     NILLDQKLVKEEKNQVLPTPYGRAVSMSFLNYNDALYIRRKFRKTKPLEIALKLEPF"
BASE COUNT          590 a          525 c          436 g          702 t
ORIGIN      
        1 taaatggttc taacttcagg gcaatttcca atggtttggt ctttctgaac ttccgtcgga
       61 tgtaaagggc atcattataa ttcagaaatg acatggaaac tgccctacca taaggggtgg
      121 gcaatacttg attcttttcc tccttgacaa gtttctgatc cagaagaata ttccaggcag
      181 attctatttc cattggaatt tcatcttttt caaattcttt ggagatttca tgaatttcag
      241 aaggccccct ggcacaaaca tcagccaaga cttgttccac caacccagct tcactgtact
      301 ggacgtgaat tggttctaca tcgctatcta agagggttac cgccatattt tcctcagtat
      361 catccccata tcttctcccc acctcgggta tgaggtaaat ctttccctgg tcatggtaac
      421 tgggacggcc tgcccggccc atcatctgag aaaactcatt gggggttaac atctccctac
      481 ccatattgaa gctttcaaat attacctgag atgctggaaa atcgactccc gcagccaggg
      541 cggctgtggt gacaaccacc gatatcttct gctccccaaa agtcttttct atcctatttt
      601 tcttagaata ggataatcca gcatggtaag ctgcggcttt aattcccctt ttaaccagat
      661 aatcagctat ggaatgagtt tttcgccgag aattggtaaa tattatgctt tgaccgtgaa
      721 atcccttctg ggaacgtttc tgaaattctg atcgggttag ttgtgtcatc cagtggttct
      781 tatcctcctc gctacgagcg aatatgaggt gtctttccag gggcactggt cttttagagt
      841 attcaaccag tttcatgcca aattcattgg caatttcctg gttgttaccc actgtagctg
      901 ataagcctat tatctgaaga tttggaaaca ggaatcgaag cctctggata agccccttaa
      961 ggcgggggcc acgctccgca tcccccaaca tgtgaatttc atcaaccacc accgtggaaa
     1021 gattgctcag ctcagatgca cggccagccc ttaaaagaaa atccagtcct tcatatgttc
     1081 ctacaacgat atcagaatct tctactttac cttttggtaa agataactct tcacgagcct
     1141 tgatacgact catacccacc tttattgaca ctttaaggtc cagttttccg tatcttttct
     1201 ggaagtcccg gtacttctgg ttggccaggg ccaccagcgg agacaagtac agaaattttc
     1261 cccccttcag ggctcgtggt atgccggcta gttcaccgat gagtgttttt ccactggctg
     1321 tggctgagac caccagcaag ttttctccag ccaggagtcc cacattaacc gccatcacct
     1381 gtacaggtag gagatgtgtg ccctgttttt ttaagataat cttaaaatta gggggaacat
     1441 ccaattcttc catatccatt ttaaaatctt tatcatcccc accccctgcc atttggtcat
     1501 agagagttag attacgttcc ttcagggggt tgaagcgagg attaaacgtt tttagaaccg
     1561 tttccaagtc tcctgtgcgg ttgagcatcc tctgaaaggt aggaaagatc tttaaatcga
     1621 aagaatgatt ctttagttcc ctttttattt cttcctcggc acaaataagg caaatgttat
     1681 cctgatgata acgaaaagat ttttcaggtt ccaataaggt tatgtgacca gtgaaggtac
     1741 aatgtcggca tatactggtt tttcggtact ttatatgtaa agattccatc aactcctcaa
     1801 cttccggttc atcccctacc agaaaaacat tctgttttct taggatctta accgcctcag
     1861 agggaggtag aatcatttcc ttatctttct gcaccacgaa gcggtgaatt ttcaactggc
     1921 ctttaattcg ccgtagtttt aaataagcat aaaataatgg tttgcgtcgc tggtttatgg
     1981 cgtccttaga acttccgatg gggaagattt ccacgatttt attctttttc ctcaagatga
     2041 tcatgtaatc aaaaaaaatt agtataaagg aattggaaga aaaacacgta taatatttct
     2101 ttactgatgg tatccctttt cggctaaata tcttcttcct gagcctttat tttttccaac
     2161 aattcaaccg cctgttccct gagtttaaat ttctgtatct tgccactggc ggttaaagga
     2221 aattcatcca caaaaaagac atgtttagga acc
//
LOCUS       DAOQVW010000245         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_59910_length_2253_cov_3.048681, whole genome shotgun sequence.
ACCESSION   DAOQVW010000245 DAOQVW010000000
VERSION     DAOQVW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59910_length_2253_cov_3.048681"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKK85_05855"
     CDS             <1..294
                     /locus_tag="PKK85_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25577.1"
                     /translation="KRVKAGDFEVPPGGGVGTALAIAHGQSVFTGGGKEIGRNTRTAP
                     GTIGINVKRCIRTGRCCSTRIMQAPLKITSVPPVHMECLGQGILLGLSTLRSR"
     gene            538..1446
                     /gene="rnz"
                     /locus_tag="PKK85_05860"
     CDS             538..1446
                     /gene="rnz"
                     /locus_tag="PKK85_05860"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNS25578.1"
                     /translation="MELVFLGTSSAIPTNHRNHPALALKAFGELMLFDCGEGTQRQMT
                     RIRMSPMKVKRIFITHLHGDHFLGLPGLIQSMAFRGREDPLYIYGPSGTGDVVESIKK
                     LGYFSLSFPIITQELYQGVFLEEDSYRISCCPVQHSVLNLAYSVEERRAPKFLREKAL
                     ELGLTPGPDFGKLQRGTPVKVGERTIQPQDVLGKKRRGRKIVYSGDTRACREMVNLAK
                     DADVLIHESTFDASQEEKALTTGHSTSKSAARVAALARVGRLILTHLSTRYRDAKKLE
                     KEASEIFPEVTVAEDFMSVEVKLRAD"
     gene            1436..2191
                     /locus_tag="PKK85_05865"
     CDS             1436..2191
                     /locus_tag="PKK85_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNS25579.1"
                     /translation="MQIDPMLEKVIFSAITLIGSYITYEVLSHFIKRIGTRLEIDLTV
                     IQVLKEIFKYTIIALAITIILNTWGVNIGPLILSLGIVGIAVGFAARDTLSNFISGMF
                     ILAEKSFRVGDTIEITGQRGKVKKMGFRVTTMTTPDNKVITVPNSAFSKSPYLNFTTM
                     TTRRVDLKINIPYSLDLEQVTPTLLNAAGSLPWACPKPKPKILIKELDDAGVKATLSV
                     WTTKPQKVGEYKTKLAQRVKGILVEEPEGKEKN"
BASE COUNT          643 a          523 c          561 g          526 t
ORIGIN      
        1 aaacgtgtca aggccggaga tttcgaagtt cctccaggag gaggcgtggg aacggcactg
       61 gcaattgccc atggacagag tgtcttcaca gggggaggta aagaaattgg gagaaacact
      121 agaacagctc ctggtaccat aggtatcaac gttaaaaggt gcattagaac tggaagatgc
      181 tgttctaccc ggattatgca agccccgtta aaaatcactt ctgtcccccc agtccacatg
      241 gaatgtctgg gtcaggggat tcttttgggt ttatcaaccc tgaggtcaag gtaggatact
      301 ggtatgtccc taatgggatg agtatggtcc ttgatgaccc cagagacctt gcccttccaa
      361 atgcaatgta tcgctccgta gaatagacca gaccctaaaa accagctaag gtctaaaaag
      421 agaagacaat gcataatata aaatatgggg aatataattt taaaggcata aaagatgcaa
      481 ctctgtcacc atccaaaacg ggttttagag ttatactgat gaaataaggt gtttatcatg
      541 gaactggtct ttctgggaac ttcatccgcc atccccacca accaccgcaa ccaccctgcc
      601 ctggctctca aggcctttgg ggagttaatg ctctttgatt gtggagaggg aacccagagg
      661 cagatgaccc gtattcgtat gagccccatg aaggttaagc gcatattcat cacccacctg
      721 catggtgacc acttcctggg actcccgggg ttgatacagt ccatggcctt ccgtggacgt
      781 gaagatcctc tctacattta cgggcccagc ggtacagggg atgtggtgga gagtataaaa
      841 aaattaggct attttagcct ctccttcccc atcatcaccc aggaattata tcagggagtt
      901 ttcctggagg aggatagtta ccggataagt tgctgtccag tgcagcactc ggttttaaac
      961 ctggcctact cagtggagga gagaagagcc cccaagtttc tacgggaaaa ggccctggaa
     1021 ctgggactga cccctggacc tgactttggc aaactgcaaa gaggaacacc ggttaaagtg
     1081 ggtgaacgca caatccagcc ccaagacgtc ctgggaaaaa aacggagagg tcggaaaata
     1141 gtctattctg gtgatacccg ggcttgtaga gagatggtta acctggcgaa ggatgcagat
     1201 gtgctcatcc acgaatcaac cttcgatgcc agtcaggaag agaaggccct gactactgga
     1261 cattccacct ccaaaagtgc cgcccgggtg gctgctttag cccgggtggg gaggctgatc
     1321 ttaacccacc ttagcaccag ataccgtgat gccaagaagt tggaaaaaga ggcctctgaa
     1381 atattcccgg aggtaactgt ggcagaagac tttatgagtg tggaggttaa gcttcgtgca
     1441 gattgatccc atgttagaga aggtgatttt cagtgccatc accctgatcg ggtcctacat
     1501 aacctatgag gtactgtctc actttataaa aagaattgga acccgcctgg aaattgattt
     1561 aacagttata caggttttaa aagagatatt caagtacacc atcattgccc tggctataac
     1621 catcattcta aatacatggg gagtgaatat aggcccattg attctcagtt tgggtattgt
     1681 agggatcgca gtgggttttg ctgccaggga tactctgtca aacttcatat ctggtatgtt
     1741 catcctggct gagaaaagct tccgggtggg ggataccatt gaaatcactg ggcagagggg
     1801 taaggtaaag aagatgggct tccgggtcac caccatgacc accccggaca acaaggtcat
     1861 caccgtaccc aactccgcct tttccaagag tccctacctt aacttcacca ccatgacaac
     1921 acggagggtg gacctaaaaa tcaacattcc ctattcactt gatctagagc aagtaacccc
     1981 cacactctta aatgctgcgg gaagccttcc ctgggcctgt cccaaaccta aaccaaagat
     2041 ccttattaaa gaacttgatg atgccggggt gaaggccacc ctgagtgtct ggaccactaa
     2101 accccaaaag gttggtgagt acaaaaccaa gctggcccag agggtgaagg gtattctggt
     2161 ggaagaacca gaaggcaagg aaaaaaatta aaatctccta cggatgaagc aggtgagtta
     2221 atgaaaagat acaatatcta ctcctttttg atc
//
LOCUS       DAOQVW010000246         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_59941_length_2252_cov_3.922622, whole genome shotgun sequence.
ACCESSION   DAOQVW010000246 DAOQVW010000000
VERSION     DAOQVW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59941_length_2252_cov_3.922622"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1824
                     /locus_tag="PKK85_05870"
     CDS             172..1824
                     /locus_tag="PKK85_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNS25580.1"
                     /translation="MRDKRKEILKDLLGEFADDDADEAETSKRSQETLRSDEESETEG
                     REEEGFFAKEDDKFNLDNLIREHSKRGSRRTKKARHMSEAFKAEIIEEGLIPKYNVAL
                     PRLSKNELDLFREIREKLVEVAVAQGEEFQVDEDSFTGEVKQFLRSKGVRDVDRMATQ
                     ISQEMLGYGKLDPMIKDDDLEEVMVIGTDRTVFVYHRKIGMMATNVVFETDDDIRAII
                     DVIARQVGRRIDQQMPILDARLKDGSRVNATIPPVSADGSTLTIRKFRKDPLTVVDLI
                     NFKTLSSHLAGFLWVCTDGMGVKPCNAIIAGGTGSGKTTTLNTVAAFVPPRERIITIE
                     DTLELQLPHTHVLRMETRPPNIEGKGELNMDTLVKNSLRQRPDRVIVGEVRGGEATTL
                     FTALNTGHSGMGTLHSNTARETITRLVNPPMSVPNIMIPALDFIIMQNRMYRSEGGSM
                     RRVTEVAEVVGMEEGNVQLNRVFEWNNVTDKVEYVGIASQTLRDIAELRGIGITEIEE
                     EIEKRRLVLEYLADNNIRSIQEVGRCVNSYYRDPDEMMDRIL"
     gene            1856..>2252
                     /locus_tag="PKK85_05875"
     CDS             1856..>2252
                     /locus_tag="PKK85_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNS25581.1"
                     /translation="MVFQGLKKFFNRIGNFTVDSSQKVGEGAQKVSSGVQTPVKKIGK
                     IKAPKIKPRKPSVKSPLKPKKKDEAEGLKFKTAATRTPKRSIKRMDMDKDEIAIFREL
                     IDKKYERRDADTSTEESQKTAIRKASLEEL"
BASE COUNT          710 a          442 c          560 g          540 t
ORIGIN      
        1 cctacccctg cttgcaaggg tgttgtgatc agttttttta tcttacatgg aaaatattat
       61 tgtaaaggca gataatttcc aatcctctag tccctggggt taggggataa tgtggagtat
      121 gattaggctt aagcttaaat atttgatacg ttatataaat aatcgagttt tatgagggac
      181 aagcgcaaag agattcttaa agatctttta ggggaatttg ctgatgatga tgcagatgaa
      241 gcagaaacat caaaaaggag tcaagaaacc ttaagatctg atgaagagtc tgaaacagag
      301 ggtagagagg aagaaggttt ttttgccaaa gaagatgaca aattcaacct ggataaccta
      361 atcagggaac attctaaaag gggctccaga cgtaccaaaa aagccaggca tatgtctgaa
      421 gcttttaaag cagaaattat tgaggaaggt ctgattccta aatataacgt tgccttgccc
      481 cgtttgtcaa aaaatgaatt agatcttttt agagaaatca gggaaaagct ggttgaagtg
      541 gcagtggccc agggtgagga attccaagtt gatgaagatt cattcaccgg tgaagtcaaa
      601 caattcctgc gcagcaaggg tgtcagggat gtggatcgga tggccaccca gatatcccag
      661 gagatgctgg gttatggcaa actggatccc atgatcaagg acgatgatct ggaagaggtc
      721 atggtgatag gaaccgatag aacggtattt gtctaccatc ggaagattgg aatgatggct
      781 accaacgttg tttttgaaac tgacgacgat atccgggcta tcatcgacgt tattgcccga
      841 caagtgggta ggaggatcga ccagcagatg ccaatcctag atgctcgttt aaaggatggt
      901 tccagggtaa acgccaccat tcccccagtt tctgctgatg gctccacttt aaccattcgt
      961 aagttccgta aggatcccct gaccgtggta gatttaatta acttcaaaac tctttcatcg
     1021 cacctggccg gcttcctgtg ggtctgtaca gatggtatgg gtgtgaagcc ctgtaacgcc
     1081 atcatagctg gaggcactgg ttcgggtaag accaccaccc tcaacactgt tgctgccttt
     1141 gtacctccca gggagcggat cataaccatt gaagacacct tggagttgca actgcctcac
     1201 acccacgttc tgcgcatgga aaccaggccc cccaacattg aaggtaaggg tgaactgaat
     1261 atggataccc tggttaagaa ctccctccgt cagagaccag accgtgtaat tgttggagaa
     1321 gttaggggag gggaagccac aactctcttc acagctttaa acaccggaca ctcgggtatg
     1381 ggaacacttc actccaacac ggctagggaa actataaccc gattggttaa ccctcctatg
     1441 agtgttccta acataatgat acctgccttg gactttataa tcatgcaaaa ccggatgtac
     1501 aggtctgaag gaggatccat gcgtcgggtc actgaggtgg ccgaagtagt aggaatggaa
     1561 gagggcaatg tccagttaaa ccgtgtattc gaatggaata acgttacaga taaagtggaa
     1621 tatgtgggta tcgccagtca gaccttaagg gatatagccg aactgagggg tataggcata
     1681 actgagatag aagaagaaat tgaaaaaaga agactggtac tggagtacct ggctgataac
     1741 aatataagat ccattcagga agtggggcgc tgtgtaaaca gttactaccg ggacccggat
     1801 gagatgatgg atagaatatt atagaatctt cccagtaaga aaaaaggtgt aaataatggt
     1861 tttccagggc cttaaaaaat ttttcaaccg tataggtaat ttcactgttg attcatccca
     1921 aaaagtgggt gaaggggctc agaaggtgag tagtggagtt caaaccccgg taaagaaaat
     1981 cggtaaaata aaagctccca agatcaagcc ccgcaaacct tctgtaaaat ctcctctcaa
     2041 acccaaaaaa aaggatgagg cagaaggcct aaaattcaag acagcagcta cccgcacacc
     2101 caagagaagt attaaacgta tggatatgga taaagatgag atcgccatat tccgggagtt
     2161 aattgataag aaatatgaga ggagggatgc tgatacatcc acggaagaat cacaaaagac
     2221 tgccatccgc aaagcatctt tggaagaact tc
//
LOCUS       DAOQVW010000247         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_60035_length_2250_cov_2.944875, whole genome shotgun sequence.
ACCESSION   DAOQVW010000247 DAOQVW010000000
VERSION     DAOQVW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60035_length_2250_cov_2.944875"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PKK85_05880"
     CDS             <1..626
                     /locus_tag="PKK85_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HNS25582.1"
                     /translation="IDVWRVLECGADPNALLRFNGTCELNHLQNFQYNPQIFQELDLD
                     LEKDKTILMRPEPALASYLQTDCRKSVLSPIVEALEAHANILVIPRFREQEEIFEDDG
                     NVTIIHPPVDTFSLMKACDLVIGAGGTMNREAALLGTPVISCYPGKLLAVDSYYIKKG
                     LMKRSTDLDDIVKISLEQILGERKELKMETDDLFQIILENIYQVALS"
     gene            complement(675..747)
                     /locus_tag="PKK85_05885"
     tRNA            complement(675..747)
                     /locus_tag="PKK85_05885"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Ala,seq:ggc)
     gene            1014..2093
                     /locus_tag="PKK85_05890"
     CDS             1014..2093
                     /locus_tag="PKK85_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25583.1"
                     /translation="MALIAETHLQYIIEVAIMLQVGVIFLLNLAPLSLSLVMMGALIV
                     GTGFAALFSLDALFLFLPGISHFEFTHPFGSLALLVIVTALAAIPAMKEAGINIRTLR
                     NYVLLLLSIITIAGGLMHRSFLILWILGLLIGFFVISKSFRQKSVFTLKRIMMVGLVV
                     VVGFGSLEILSRVLEMNILSPLLRLMRIDTYAMPSVKMVIKNATLTGHQLGACYWGDT
                     CLGGSDGYMSLPISLITLFGLPFPLFFGILVTKKDVIDYMLPGIFGVTFDFGYLFLFF
                     LLLWCAGVMVVGARMLRTYRSRRENGDKSCLGREALLIGSITAFMAQTLVGLFIMNRS
                     INGTAMLTFLVLSAMVVGHIIFIKK"
     gene            2122..>2250
                     /locus_tag="PKK85_05895"
     CDS             2122..>2250
                     /locus_tag="PKK85_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 family protein"
                     /protein_id="HNS25584.1"
                     /translation="MKKALILLGCPESPSQTPLAIYAAYKLDKMGFEVTLASTPSAM"
BASE COUNT          632 a          440 c          493 g          685 t
ORIGIN      
        1 ccattgacgt gtggcgtgtg ttggagtgtg gggctgatcc caacgctttg ctgcgtttta
       61 atggtacatg tgaacttaat caccttcaaa atttccagta caatccccaa atattccaag
      121 aacttgatct ggatctggaa aaggacaaaa caattctcat gcgacctgaa cctgcacttg
      181 catcttatct acagactgac tgtcgcaaat cggtgttatc acctatagtg gaggccctgg
      241 aggcccatgc taatatactg gtcattcccc gcttccggga acaggaagaa atcttcgaag
      301 atgatgggaa cgttactata atacatcccc cagtggacac ctttagcctg atgaaggctt
      361 gtgacctggt aattggagcg gggggcacga tgaaccgaga agcagctctt ctgggcaccc
      421 cagtaatatc ttgttatcct ggtaaattac tggctgtgga tagttattac attaaaaaag
      481 ggcttatgaa acgctccaca gatttggatg atattgtaaa aatctcccta gaacagatct
      541 taggggagag gaaagaactt aaaatggaaa ccgatgatct gttccagata atcttggaaa
      601 atatatacca agttgcatta agctaaattt ctatctaatc aaatattatt ggcatcagaa
      661 ttagttgtta taaatggact ggccgggatt tgaacccggg gcctccgcca tgccaaggcg
      721 acactctacc gactgagcta ccagcccaca tagcaaatag gacttagggg tatttaaact
      781 tagtgttaat gtttctaatc catcacactc gtaaagcttt taaatagatg tctttaatat
      841 aactagctct gaactaatat taataaaaaa tgggggcctt gaaagcaccc tatcttataa
      901 agaaaatctg ggttttaaac tcttaattat aaaattgatg ttaaaaaaat ttttttaagc
      961 ttattaaagg aatttggctc ttaaaaaagt ggcacctaca ttggagaatt cacatggcct
     1021 taatcgcgga aactcatctt caatacatta tagaagtagc aataatgctg caagtagggg
     1081 tgatattcct cttgaatctg gccccactct ctttaagctt ggtcatgatg ggtgccctta
     1141 ttgtgggaac tggttttgca gcccttttca gtttggatgc cttgtttcta tttttacccg
     1201 gaataagtca ctttgaattc acacacccct ttggttcttt ggctcttttg gtcatagtaa
     1261 ctgctttggc agctattcct gcaatgaaag aggcgggtat taacatcagg actttgagga
     1321 actatgtgct attacttcta tccataataa ccatagctgg gggtttaatg catcggtcgt
     1381 tccttattct atggatttta gggctgctaa taggcttttt cgtaatatcc aaatcgttcc
     1441 gacaaaaatc agtcttcact ctgaagagaa taatgatggt gggactggtg gtagtagttg
     1501 gatttggatc tctagagatc ctgtcgaggg tgctggaaat gaacatactc agtccccttc
     1561 ttcgtttgat gcgtattgat acctatgcca tgcccagtgt gaaaatggtt ataaaaaacg
     1621 ctactttaac cggtcaccaa ctgggtgcct gttactgggg ggatacgtgt ttggggggtt
     1681 ctgatggata catgtctttg cccatatccc tgataaccct atttggttta ccatttccat
     1741 tgttttttgg aattctggtt accaagaagg atgtaattga ctacatgtta ccgggaatat
     1801 tcggggtgac ctttgacttt ggctacttgt tcctattctt ccttctcctg tggtgtgccg
     1861 gtgtgatggt ggtgggtgct aggatgctaa gaacataccg ttcaagaaga gaaaatggtg
     1921 ataaaagctg ccttggaaga gaagcacttc tgataggttc tattacggct ttcatggctc
     1981 agactctggt gggattgttt ataatgaacc gttccataaa cggaactgcc atgctcactt
     2041 ttttggtcct gagtgccatg gtggtgggtc acatcatatt cataaaaaaa taaatagaac
     2101 aaaaataata ggagatagta gatgaaaaag gcacttatac ttttaggatg tccagaatca
     2161 ccttctcaaa ctccattagc catatatgct gcgtataaac ttgacaaaat gggttttgaa
     2221 gtcacactgg ccagcacacc atcggccatg
//
LOCUS       DAOQVW010000248         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_60078_length_2249_cov_3.114403, whole genome shotgun sequence.
ACCESSION   DAOQVW010000248 DAOQVW010000000
VERSION     DAOQVW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60078_length_2249_cov_3.114403"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..903)
                     /locus_tag="PKK85_05900"
     CDS             complement(157..903)
                     /locus_tag="PKK85_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS25585.1"
                     /translation="MLSDKERLAVFFEAINKKVQGIVYDLGTGSGVLAAEAAPRAKHV
                     YAIEKNELSARSALNRLSYFNNVSVIVGDASLMDYPEKADLIICEMLDTGLIDEEMIP
                     VINHARRYLHEGGHIIPCEVLNGVEAINLGAEHVCYQDCYQDRVVENFKTMGPLKIYN
                     RINLLKEIDEKVDLTLKLKTNSHGNVNGIKITTFTLLTPNLICGPTPMLNPPLLVPCN
                     LLTVEKNQEIRLNLKYIMGGGLSTIEARIN"
     gene            complement(1044..1454)
                     /gene="nikR"
                     /locus_tag="PKK85_05905"
     CDS             complement(1044..1454)
                     /gene="nikR"
                     /locus_tag="PKK85_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNS25586.1"
                     /translation="MMRISMSLPKKLLNEFDEVLKDRGYQSRSKGIRDALKDYIVRYQ
                     WMNEMEGDRIGILAVIYDHHYTGVMEDLTDIQHDYRKFINAVMHVHMTEKYCLEVIVV
                     KGNVNYIRELTEKIMRLKGVEHVKLTSTSSGDNM"
     gene            1857..>2249
                     /locus_tag="PKK85_05910"
     CDS             1857..>2249
                     /locus_tag="PKK85_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS25587.1"
                     /translation="MKVREAMNQGVISISAQTSPLEAFEKMYKEGVRRLFVVNEEDGV
                     LGVVSYSDLIGVLGAIKPTIRKEVSLNLMDIMSKDVITISAGDGIEDAANLMLRADIS
                     GLLVLEDKKPVGVISKTDICRLVAAEILV"
BASE COUNT          645 a          415 c          421 g          768 t
ORIGIN      
        1 ccagtaaagt tttctggaac aactccccca tctatggata ttatttgggg gtgcaagttg
       61 tttaaacttc tggcaatgaa tgttcccagg gcatcatctc ccttcaagtc atttccaatg
      121 cccagtatta ctaatttttc gtatcccaac aaaaattcag ttaattcttg cttcaatggt
      181 acttaatcca cctcccatga tgtatttaag atttaaacgt atttcttggt ttttttcaac
      241 tgtcaataaa ttgcagggca ccagtaacgg tggattgagc atgggtgtgg gtccacagat
      301 taaatttggg gtgagcaggg tgaaagtagt gatttttatt ccatttacat ttccatggga
      361 atttgtcttg agtttcaaag tcagatctac cttttcatca atttctttca acagatttat
      421 gcgattatat attttaagag gccccatggt cttgaaattt tctaccaccc tgtcctggta
      481 acaatcctgg taacatacat gttctgcacc taaattaatg gcctcgacac cgtttaaaac
      541 ttcacaaggt attatgtgcc ctccttcgtg gagatatctc cgggcatggt tgatgaccgg
      601 tatcatttct tcatcaatga ggccagtgtc cagcatctca cagattatca gatcagcttt
      661 ttctggataa tccattagac ttgcatcacc cactatcacg gagacattgt taaaataaga
      721 aagtctgttt aaggcggatc tggctgacaa ttcatttttt tcgatggcat aaacatgttt
      781 tgctcgtgga gctgcttcag cggcaagaac tccggaacca gttcctagat catagacaat
      841 tccctgcact ttcttgttga tggcttcaaa aaaaactgct aggcgttctt tgtcagatag
      901 caatttttct tgataggggg ttagtttcat caatgtatag tgaatattga ttctattaat
      961 aaaaattctg atggtaaaaa taagtttaat ttcaagaatg gtgtgaagaa aattttatta
     1021 gggggaagat ttggtaaaag tagttacata ttatccccgc tggacgtact ggtgagtttg
     1081 acgtgttcaa cgccctttaa tctcattatt ttttcggtaa gttcccgtat gtaattgaca
     1141 ttccccttaa ccacaataac ttctagacaa tatttttctg tcatgtggac gtgcataaca
     1201 gcattgatga attttcggta gtcatgttgg atatcggtga ggtcttccat tactccagta
     1261 tagtgatgat cgtaaatcac agctagaata ccgattcgat caccttccat ttcattcatc
     1321 cactggtatc gcacaatgta gtcttttaaa gcgtctctta taccttttga tcttgattgg
     1381 tatcctcgat ctttcaaaac ttcatcaaac tcgtttaaaa gtttcttggg taaggacata
     1441 cttattcgca tcattacaat tccctcgtat tattttatta taatattatc ctatttaaat
     1501 ctttgttttc ttagcaatat tgttcactac aaacaatttg tatgtcttcg gggatactgg
     1561 tttagcatca cggacaaaaa attaacacta gcactattat attactggta atgctattgt
     1621 tactatttaa actttgccta cctcgaaaac attgatgaca tttaacaaag ttgcttagat
     1681 ctaagccaga gtcaggtcat attaaatctt tattaataat cctttccaat tataaacaac
     1741 tcttcttggt tagccttttt caaatacttc attaattttt ccattaaaaa ccaatactat
     1801 taatcttgtg tttgacaaca tatcaattaa cagaattata tttatgtggt gatactatga
     1861 aagtgaggga ggccatgaat caaggggtga tatccatcag cgcccaaacc agcccgttag
     1921 aagcctttga aaagatgtat aaggaaggtg tgcgtagatt atttgtggtg aatgaagaag
     1981 atggtgtttt gggagtggta tcctattctg accttattgg agtactggga gccataaaac
     2041 ctactattcg gaaagaagtg tcccttaacc tgatggatat catgtccaag gatgtaataa
     2101 ccatttctgc cggtgatgga attgaagatg cagccaattt gatgttacga gcagacatat
     2161 ctggactact ggtcttggag gataaaaagc ctgtaggagt aattagtaaa acagacatat
     2221 gtcggttggt ggctgctgaa attctggtt
//
LOCUS       DAOQVW010000249         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_60318_length_2243_cov_6.526051, whole genome shotgun sequence.
ACCESSION   DAOQVW010000249 DAOQVW010000000
VERSION     DAOQVW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60318_length_2243_cov_6.526051"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PKK85_05915"
     CDS             complement(<1..909)
                     /locus_tag="PKK85_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25588.1"
                     /translation="MEVQTRNTAETVSKQELLRYAMENPVAVSRALNDRSLFHFLEYF
                     WDVVSAHSFHPNWHIEFLCHELETVAQRVGDRRAREYDMIINVPPGTTKTITCSIMFP
                     VWCWTRWPWMRFICASYSNTLSLESAEYCRDLIRSQKFREMYPDLDIRDDKDTKSNFK
                     IVKKVMQPGMPPKIISGGSRYSTSVGGTLTGFHGDILIVDDPLNPNQAVSELELAAAN
                     RWCEQTLSTRKTDKAITPTIFIMQRLHQDDPAGHILSKRKENIRHICLPGDARRYKEK
                     INPPELYQYYKDDLLDPVRLPWKVLED"
     gene            complement(916..1392)
                     /locus_tag="PKK85_05920"
     CDS             complement(916..1392)
                     /locus_tag="PKK85_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25589.1"
                     /translation="MWSNGFPQIAFEMALLGAEDKDIARAFGVTKDQFLKWVENKAEL
                     AQALSEGKLLADSKVAASLYKRATGFSYEEEVLHVIKGEVVRTVVTKHVIPDPWSAHK
                     WLSARQPQLWTDTSKSEITHTNININKFDFGALTTEELQLVERIGLRQIAQNIALG"
BASE COUNT          609 a          541 c          437 g          656 t
ORIGIN      
        1 gtcctcaaga actttccatg gtaaccggac agggtccaac agatcatcct tgtaatactg
       61 gtagagttca ggtggattta tcttttcctt atacctgcgt gcatctccag gcaggcatat
      121 atgacggatg ttttctttac gcttggaaag aatatgacca gcaggatcat cctgatgcaa
      181 tcgctgcata atgaagatag ttggcgttat ggccttatcc gtcttacggg tggacagcgt
      241 ttgttcacac cagcggttag ctgctgcaag ttccagttcg ctcacggcct gattaggatt
      301 cagggggtcg tccacaatca gaatgtctcc atgaaacccc gtcaaagtac ccccgactga
      361 cgtgctataa cggctacctc cagatatgat cttgggaggc atgcctggtt gcatcacctt
      421 ttttacgatc ttgaagttac tcttggtatc cttatcatca cgaatatcca gatcaggata
      481 catttcacgg aacttctgtg aacgtatgag gtcacggcaa tactctgcac tttccaagga
      541 aagggtgtta gagtatgatg cacaaataaa cctcatccac ggccaccgtg tccaacacca
      601 gaccgggaac ataattgagc acgtgatggt tttagtcgtt ccaggtggaa cgttaatgat
      661 catatcgtat tcccgtgccc tgcggtctcc aacacgctgt gcaactgttt ccagttcatg
      721 gcacaggaac tcgatatgcc agttgggatg aaatgagtgt gcactaacaa catcccagaa
      781 atattcaagg aaatggaaga ggctacgatc attaagtgca cgtgacactg ccaccgggtt
      841 ctccatagca taacgtaata gttcctgctt ggaaacggtt tcagcggtat ttcgggtttg
      901 cacttccatg attttctaac ctaatgcaat attttgagcg atctggcgca gaccgattct
      961 ttccaccagt tgcaattctt cagtagttaa tgcaccaaaa tcgaatttgt tgatgttaat
     1021 atttgtatga gtaatctcac tcttactagt atcagtccat aactggggct gacgtgcact
     1081 cagccactta tgtgcactcc atggatctgg gatcacatgt ttggtcacca ccgtgcgaac
     1141 cacttccccc ttgatcacat gcaaaacttc ctcttcatat gaaaatccag ttgcacgctt
     1201 atacagtgat gcagccacct tggaatctgc aagtaatttt ccttcagaaa gtgcctgggc
     1261 cagttcagcc ttattttcca cccacttgag aaactgatct ttagttactc caaatgcacg
     1321 tgcaatatct ttatcttcag ctccaagtaa cgccatctca aatgcaatct gcgggaatcc
     1381 attactccac aacagcggcc tacccacttt tccagtcctg tttctagatc gcctcatctc
     1441 cagttaaatt tacttgattg cgtaaattta atggattgaa accatataac gtatacactt
     1501 gcacaattat ttttacgaag ttcactgcat caaaaaatcc atcaacactc catataatac
     1561 aaatcccacc aacgtgcacc caaaaaaaaa ttttgatttt ctttaatacc ttcttaaaaa
     1621 gaacagataa tatgcatgat aagaaataat atcacaaaaa tccccctata ataacagagt
     1681 aattacccta ttacttccct accgatatgc atatggaaat ttaccatatg aactggaaac
     1741 gagttgcaaa aaccacttga aaaccaaatt tggactgcag aattttcgtg actgcattcg
     1801 gccgagttca taaaatgaat ctgccgatgg accgggggtg gtggctcatc ccccactcct
     1861 tccaccatca gcggtggctc cagggacctt ccacgatacg tggcttgtcc ggtggatatg
     1921 cattggaggc atagccatta tgcacgtcta aactatcatc atacggcgtg taatattata
     1981 cggaccaagc cacatgcatg cggtgcccgt ccacacccgt gctaggcatt tttaataggt
     2041 tactataggg tgatattata ggttatttcg acatgacgtt ttatacctta ttccgatgtg
     2101 ataataggat acggatacgg gatgttaata gctttattta tcacccgttt tttactttcc
     2161 tctagcttat accttattcc aggatatccg gagtagttat attatcccgt tttttccact
     2221 tttcgtgccg tttccggtcc ttt
//
LOCUS       DAOQVW010000250         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_60391_length_2242_cov_2.654321, whole genome shotgun sequence.
ACCESSION   DAOQVW010000250 DAOQVW010000000
VERSION     DAOQVW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60391_length_2242_cov_2.654321"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKK85_05925"
     CDS             <1..249
                     /locus_tag="PKK85_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA synthase subunit gamma"
                     /protein_id="HNS25590.1"
                     /translation="LDTEGRAVDVSLAGGQLNAEAVADLIKETGVEEKVKTRTLIIPG
                     LAAPVSGEIEDESGWEVLVGPRDSSAVPGFLSDLKEKS"
     gene            273..743
                     /locus_tag="PKK85_05930"
     CDS             273..743
                     /locus_tag="PKK85_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS25591.1"
                     /translation="MKTLMVVDPRRCSECQDCINACQKEHGVARVKKSSTVPIFCMHC
                     HPDKAPCARICPTGAIKEDEGTLMVDEDACILCRLCMIACPVGMMIIDEDHKSVQKCT
                     LCLDAEGVIPACVDACKDNVLKIFSVEDLEELKKDLSFTEVLNEAMKVYQDKVG"
     gene            complement(854..2209)
                     /locus_tag="PKK85_05935"
     CDS             complement(854..2209)
                     /locus_tag="PKK85_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS25592.1"
                     /translation="MNSLEPDIDELIENGDVEGLIRALYDDDYITRKEATFGLKKVGD
                     KRAVEHLINSLLYKNWHKDYTILTGVRENSAEALGMIGEESAIDALIQALEEDPDEEV
                     RWKAVWVLGEIGNIQVTEALIKALKDNDWTVRRHAANSLGMIGDEEAVPSIIKALNDR
                     DWHVRKYAAVALGKMKDERAIPILLEALDDDDADVRWKAASSLVKMGEISVPLVINVF
                     KTGSWRVRGRAAEILGKIGDERAVPVLIDSLSHGKSLEKHRHVRGKAAEALGNIGDKV
                     ALDILLKTFHEDEYKYVQDKAEEAINKIRKTAKERSILTYDNGEVSFNYPGEWDLMDT
                     PDPKKIVKGYYANSAINLSLNRQADVEDISSDEFAEMLRNVLSIQNSTIISEDMFNRD
                     GMEVYQVVAENNEVVPTDILAVSYKKLGLLYYLWFAGDPEVLKHAEEGIQIMLDTFYI
                     Y"
BASE COUNT          572 a          524 c          499 g          647 t
ORIGIN      
        1 ctggatactg agggacgtgc ggtggatgtt tccctggctg gtggtcagct taatgccgag
       61 gcagtggctg atctcattaa agaaactggt gtggaagaaa aggtaaaaac acgaactctg
      121 attatccctg gactggctgc tccggtcagt ggtgaaattg aggatgaaag tggatgggaa
      181 gttctggtgg gccccagaga ctcttcagct gtacctggct tcctatccga cctgaaagaa
      241 aaatcttaat tggatatagg gaagtgatta acatgaaaac cttgatggtg gtggatccca
      301 ggcgctgcag tgaatgtcaa gactgtataa acgcctgcca gaaggaacac ggagtggctc
      361 gtgtaaaaaa aagcagcaca gtgcccattt tctgtatgca ctgccaccca gataaagcac
      421 catgtgccag gatatgccca accggagcca ttaaagagga tgaaggcaca ctgatggtgg
      481 atgaggatgc atgtatactc tgccgcctgt gcatgatagc ctgtccagtg gggatgatga
      541 tcatcgatga agaccacaaa tctgtgcaga agtgcaccct gtgccttgat gccgagggcg
      601 ttataccggc ctgtgtagat gcctgtaaag acaacgttct gaagatcttc tcggtggagg
      661 atctggagga gcttaagaag gatctttcct ttactgaagt cttgaacgaa gcaatgaagg
      721 tctaccagga caaggtagga tgatgagggc acggccaaag ggatgatctg ggctcctctc
      781 tttttttttt aaataaacag ttgataattt aaataaatca aaaaaggaat aaaaacaagg
      841 aattatggga tgattaatag atgtagaatg tatctagcat gatctgaatt ccttcttcag
      901 catgtttcag tacttcagga tcccctgcaa accagaggta gtacaggaga cctaactttt
      961 tataggaaac agccagtata tcagtgggaa ccacctcgtt gttctcggcc accacttggt
     1021 aaacttccat gccatcccgg ttgaacatgt cctcagagat gatggtactg ttctggatgg
     1081 acagcacatt cctcaacatc tcggcaaatt catcagagct tatatcttct acatctgctt
     1141 gtctgttaag ggataggtta atggcgctgt tggcatagta acccttaaca atttttttgg
     1201 gatcaggggt gtccattaga tcccattccc cgggatagtt aaaggaaact tcgccattgt
     1261 cgtaggtgag tatacttctt tcctttgcag tttttctaat tttatttatg gcctcttctg
     1321 ccttgtcctg gacgtattta tattcatctt cgtggaaagt cttcaggagg atgtctaaag
     1381 ctactttgtc acctatattt cctagtgctt ctgcagcttt tcctctaacg tgtctatgtt
     1441 tctctaatga cttcccatgt gataaagaat caattaaaac cggaactgct ctttcgtccc
     1501 ctattttccc cagaatttca gctgctcttc cccgtacacg ccagcttcca gttttgaaga
     1561 cgtttataac aagaggaacc gatatttccc ccatcttcac cagggatgat gctgccttcc
     1621 atctcacatc cgcatcatca tcgtccaggg cctctaaaag gatggggatg gcccgttcat
     1681 ccttcatctt acccagagcc actgcggcat atttacgcac gtgccagtcc cggtcattaa
     1741 gggccttaat tatggatgga accgcctctt catctccgat catacccagg gaattggcgg
     1801 cgtgtctccg cacagtccaa tcattatctt tcagggcttt aatcagggct tcagtgacct
     1861 ggatgttccc aatttctccc aaaacccaga cagctttcca gcgtacttct tcgtcaggat
     1921 cttcttctaa agcctggatt agggcgtcaa tggcggattc ctctccgatc atccccaaag
     1981 cttctgcaga attctctcta accccggtta atatagtgta atctttatgc cagtttttgt
     2041 atagtagaga attaatcagg tgctccacag ccctcttatc acccaccttt ttaagaccaa
     2101 aggtggcttc ttttcgggtg atgtaatcat catcatataa agcccggatt agtccttcca
     2161 cgtccccatt ctctattagt tcatcaatat ctggttctaa tgagttcatg attgatctgc
     2221 cccccttcta tattatctcc tc
//
LOCUS       DAOQVW010000251         2236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_60625_length_2236_cov_3.280147, whole genome shotgun sequence.
ACCESSION   DAOQVW010000251 DAOQVW010000000
VERSION     DAOQVW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60625_length_2236_cov_3.280147"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="PKK85_05940"
     CDS             <1..1346
                     /locus_tag="PKK85_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS25593.1"
                     /translation="SHANIGIIFVDGPCLFSSVEYSITPKGREKARAINEDNPYIGMA
                     PVSYEDYYEIMDVQMKGRYPIKIPTKVVDNTFKDVVGIAYAKESIIESCIIGKGIFVY
                     GPPGTGKTFVVSKMPDLLPPLIIPKFIEFGGRIIQIYDSSFHKKCEEQPEDPRWVKIY
                     APFVLTGAELNLNKLETNYNPNKGVYETSPIIKANGGILLVDDLGRQRDDHELILNRL
                     IVPMENNQDMIYVRGIPVVVHSHFIPAFSTNLDISIMDEAHLRRAPLHIFLKNPDVEE
                     VKEVFRRNLEQLQEDYEKDVLDRFSKVYKKLNEGGEGLQPSFAHARDVAQICQAVRIN
                     KDKNIIDIEVLEEALEKHVLIVLQRMKIDISQIANKTRSFRIKTLDVEAASQALSDFG
                     SIVISTEQDSLIADLEENITPVVVADYLRKRGIDVKKIEVIAESEKELRRTILGW"
     gene            1533..>2236
                     /locus_tag="PKK85_05945"
     CDS             1533..>2236
                     /locus_tag="PKK85_05945"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNS25594.1"
                     /translation="MLGIIVLAISLSFSSVDTEKAHFNNEEISFDYPSNWQQVNSSDS
                     KVIILEEPNSRFKLEVNIEVTPQEWVKPENFINKLSESGEYRLKLLKHEKRKINGTLA
                     YENTYQLGGLQDMTQIREVWLEKNNVIYSIIFVNPHKARENFLKNSINSNQHEIDMVL
                     ESFTVSNLQHLDDRVWGEILIPSQGTSWKIRSDTVNEYNSVYHYPESSYTGFNGTVGL
                     LGHHTLYSAPFANIPL"
BASE COUNT          747 a          429 c          445 g          615 t
ORIGIN      
        1 ggagccatgc caatataggg attatcttcg ttgatggccc gtgccttttt tcaagcgtgg
       61 aatacagtat aacccccaaa ggacgagaaa aggcacgggc catcaacgaa gataatccct
      121 atattggcat ggctccggtg tcctatgagg attactatga aataatggat gtgcaaatga
      181 agggccgcta ccccatcaag atcccaacca aagtggtgga taataccttc aaagatgtgg
      241 tgggtattgc atacgctaaa gagtccataa ttgaatcctg cattattggt aagggtattt
      301 ttgtatacgg ccctcctggt actggtaaaa catttgtagt aagtaaaatg ccggatttac
      361 taccgcccct gataatccca aagtttatag aatttggagg ccgaatcatc cagatctatg
      421 actccagttt ccacaaaaaa tgtgaagagc aaccagaaga ccctcgatgg gttaagatat
      481 acgccccatt tgttcttacc ggtgcagaac ttaatttaaa caaacttgag actaactaca
      541 atcccaataa gggggtttac gaaacttccc ctattattaa agctaatggt ggaattttac
      601 ttgttgatga cctgggcagg caaagggatg accacgaact cattcttaac cgcctcatag
      661 tacctatgga aaacaaccag gacatgatct acgtgcgagg aataccagtt gtggtacact
      721 cccactttat tccggcattc tccaccaacc tggacatcag catcatggat gaagcccatc
      781 tacgtcgagc tccactgcat atcttcctta aaaatccgga tgtagaggaa gtaaaagaag
      841 tatttcgccg gaatctggaa caacttcagg aagactatga aaaagatgtt ctggaccgtt
      901 tcagtaaggt ttacaaaaaa ctaaatgagg gcggtgaagg attgcaaccc agctttgccc
      961 atgcccgtga tgtagcccag atatgtcagg cagtgcgtat taacaaggac aaaaacatta
     1021 tagatattga agtcctagaa gaggcacttg aaaagcacgt ccttattgta ctgcaaagga
     1081 tgaaaattga tattagccaa atcgccaata aaacccggtc cttccgtata aaaacccttg
     1141 atgttgaagc agcatcacaa gcactatccg actttggatc cattgtaatt tccacagaac
     1201 aggattcttt gattgcggat ctagaagaaa atatcactcc ggttgtcgtg gcagattacc
     1261 tgagaaaacg gggcatcgat gttaaaaaga tcgaagtgat tgctgaatca gaaaaagaac
     1321 ttcgaagaac cattcttgga tggtgaattt aaaattcatt ttttactcta aataagttga
     1381 attaaagaaa ttgattacta ctaattattc ctaattaaaa aaaaggcgta aatttatcaa
     1441 atcctgaaaa aaattaacat tgaaagaagt aaaatacgat aaagtctgtg gttttcttga
     1501 aacatccaca ttttggaata ataggagttt tgttgctggg aataattgtt ttagccattt
     1561 ccttgtcttt tagcagtgtg gatactgaaa aagcccattt taataatgaa gaaatatctt
     1621 ttgattatcc atcaaattgg cagcaagtta actcttcaga ttccaaggtg attatcttgg
     1681 aagagcctaa ttccagattt aaattggaag taaacatcga agtaactcct caagaatggg
     1741 taaagccaga aaatttcatt aacaaactat ctgaatctgg tgaatatagg ttaaagttat
     1801 taaaacatga aaaaagaaag ataaacggta ccttggctta cgaaaatacc tatcaattgg
     1861 ggggccttca agacatgacc cagatcagag aagtttggtt ggagaaaaat aacgtaatct
     1921 acagtattat attcgtcaat ccgcacaaag ctagggaaaa ctttcttaaa aactcgataa
     1981 acagcaatca acatgaaata gatatggtgc tggaaagctt tactgtatcc aatctccaac
     2041 atcttgatga tagggtatgg ggagagattt taattccttc ccaggggacg agttggaaaa
     2101 ttcgttccga tactgtaaat gagtataact cagtttatca ctatcctgaa agctcttaca
     2161 ccggttttaa tggaactgtt ggactattag gccatcacac attatattcc gccccctttg
     2221 ccaatattcc tctttt
//
LOCUS       DAOQVW010000252         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_60867_length_2230_cov_3.481839, whole genome shotgun sequence.
ACCESSION   DAOQVW010000252 DAOQVW010000000
VERSION     DAOQVW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60867_length_2230_cov_3.481839"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PKK85_05950"
     CDS             <1..697
                     /locus_tag="PKK85_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNS25595.1"
                     /translation="LERGGKVLVPVFAVGRAQELMVVLEEYMRHGIIDEVPIYIDGMI
                     WEANAIHTARPEYLSKDLRDQIFHMGRNPFISDVFHKVNGMEKRRDIIEGEPSIILST
                     SGMLTGGNSVEYFKWLCEDEKNSLVFVGYQSEGSMGRRIQKGWKEIPLKEDGKTNVYN
                     VKMQITTIEGFSGHSDRKQLMDYIRRISPKPEKILVCHGDNYKTLDLASSIYRNFKIE
                     TKTPMNLETVRIQ"
     gene            816..1841
                     /gene="purM"
                     /locus_tag="PKK85_05955"
     CDS             816..1841
                     /gene="purM"
                     /locus_tag="PKK85_05955"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNS25596.1"
                     /translation="MVTYSESGVDMGLEEVTVAAMVSQLKSTLELQDVITDTGHFAAL
                     LKLGDKALAMSTDGVGSKILVAELMDKYDTVGIDCVAMVVNDILCVGAQPLAMVDYLA
                     VEKPDPEIASQLGKGLAQGAIDAKIAMIGGETASLPGIVHNFDLAGAGIGVVDINGII
                     SGEDIQDGDVVVGVGSSGIHSNGLSLARKVFLEEAQLKVTDPLPGYPNFSVGEVLLEP
                     TRIYVKPVMDLLCEVEVHGLAHITGSGFNNLKRLKKGLSYNLNNLPEPMPIFKSIYSL
                     GVNLEEMYRVFNMGVGFVIITTPDNVEKTLKIVEKYYPAQEIGNVVANGKGCVKLKTM
                     EGSWIEL"
     gene            1855..>2230
                     /locus_tag="PKK85_05960"
     CDS             1855..>2230
                     /locus_tag="PKK85_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNS25597.1"
                     /translation="MRITIKQELYLITEILTRMGVSEEDAAIVAEVTADADLKGFSSH
                     GIGRFPQYIKGLKMGTINPQAEVTVERESPATALLKGNHGFGHVIAYKGMKMAIEKAK
                     NMGIGMVGINNSNHFGIAGYYSD"
BASE COUNT          706 a          401 c          551 g          572 t
ORIGIN      
        1 cctggaaagg ggaggtaagg tactggttcc agtgtttgca gtgggaaggg ctcaggagtt
       61 gatggtagtt ctggaagagt atatgaggca tggaatcatt gatgaagtcc cgatatacat
      121 tgacgggatg atctgggaag ctaacgccat ccatacagct cgaccagagt atctgagtaa
      181 ggatttaagg gatcagatat tccatatggg tcgaaatccc tttatatccg atgtattcca
      241 taaagttaac gggatggaaa aacgccgaga catcatagaa ggagaaccat ccattattct
      301 atccacttct ggtatgctca ccggtgggaa ctcggttgaa tatttcaaat ggctgtgtga
      361 agatgaaaaa aacagcttgg tctttgtagg atatcaatct gaaggttcta tgggaaggag
      421 aatacagaag ggatggaagg aaataccctt aaaagaagat ggaaaaacca acgtttacaa
      481 cgttaaaatg cagataacaa ccattgaagg tttcagtggt cattctgacc gtaaacaatt
      541 aatggattac atcaggcgaa tttcccctaa gcccgagaag atcctagtct gccatggtga
      601 caactacaaa accctggatc tggccagcag catctaccgt aactttaaaa tcgaaacaaa
      661 aactcccatg aatttggaaa cagtgaggat acagtgaatt aaaggaccac aacataagtt
      721 aatgggcatt gactggatta agtttgttcc cctatgatga tctttgagaa atcttcgttt
      781 taattcaaca atcaaaaaaa aatattggtg atgtaatggt aacctattct gaatcaggag
      841 tagatatggg cctggaagag gtcacagtgg cagctatggt atcccaactt aagtctaccc
      901 tagaacttca agatgtgatc accgacaccg gtcacttcgc tgctctttta aaacttgggg
      961 ataaagcctt ggccatgagc accgatggcg tgggaagtaa aatactggtg gcggagctta
     1021 tggataagta tgacactgtg ggtattgatt gtgtggccat ggtagtgaat gacatccttt
     1081 gcgtgggtgc ccagccactg gccatggtgg attatctggc cgtggaaaaa ccagaccctg
     1141 aaatcgcgtc tcaactaggt aaaggacttg ctcaaggagc tatagatgct aaaatcgcta
     1201 tgattggtgg tgaaactgct tccctacctg gaattgtgca taattttgac ttagcaggtg
     1261 caggtatcgg agtggtggat atcaatggaa ttattagtgg tgaagatata caggatgggg
     1321 atgtcgtagt tggtgtcgga agtagtggta ttcacagtaa tggattgagc ctggccagaa
     1381 aggtcttctt ggaagaagct cagcttaaag taacagatcc actgccgggt taccctaatt
     1441 tcagcgtggg ggaggtgctt cttgaaccga cgcgcatcta cgttaagcca gttatggatc
     1501 ttctatgtga agtggaggtg catggcttgg cccacattac aggaagtggc tttaacaacc
     1561 ttaaaagact gaaaaaaggg ttgagttata atttaaataa tttacctgaa cctatgccaa
     1621 tttttaagtc aatttattca cttggagtta atttggagga aatgtaccgc gttttcaata
     1681 tgggggtggg ttttgttatc ataaccactc cagataacgt tgaaaagact ttaaaaatcg
     1741 ttgaaaaata ctatcctgcc caggagatag gtaatgtagt ggccaatggg aaaggatgtg
     1801 ttaaactcaa aacaatggaa ggatcatgga tagaacttta aaggaggttt tgtaatgaga
     1861 attaccatca aacaggaact atacctaata acagaaattc ttactagaat gggagtatca
     1921 gaagaagacg ctgccatcgt ggccgaagta accgctgacg ctgatttaaa aggtttttca
     1981 tcccatggta ttggcagatt cccccagtat atcaaaggac tcaaaatggg caccatcaac
     2041 ccccaagcag aagtaacagt agaaagggaa agcccagcca cagccttatt aaaaggaaac
     2101 catggctttg gacacgttat agcctacaaa ggaatgaaga tggccataga aaaggcgaaa
     2161 aacatgggta taggtatggt gggcatcaat aactccaacc atttcgggat agcaggctac
     2221 tactccgata
//
LOCUS       DAOQVW010000253         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_60911_length_2229_cov_3.169733, whole genome shotgun sequence.
ACCESSION   DAOQVW010000253 DAOQVW010000000
VERSION     DAOQVW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60911_length_2229_cov_3.169733"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..419)
                     /locus_tag="PKK85_05965"
     CDS             complement(96..419)
                     /locus_tag="PKK85_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS25598.1"
                     /translation="MKEDVFYGKGVHHAKKDYPDIYDALVALNEATYTGKVLDYRTQK
                     LIALGITAGASDDRAMKKQMQSAIQEFGVSKDEIVDVLRVVLLTAGNPPFSKAMRILY
                     EVLEE"
     gene            complement(678..1136)
                     /gene="ribC"
                     /locus_tag="PKK85_05970"
     CDS             complement(678..1136)
                     /gene="ribC"
                     /locus_tag="PKK85_05970"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNS25599.1"
                     /translation="MKIGICDTTFARYDMASAAVDEIKQHIGNLKIIRRTVPGVKDLP
                     VACKKLIEEEGCEMVMALGMPGPEKIDKICAHEASTGLIQAQLMTNTHILEVFVHEDE
                     GKDEKDLKFLADNRAREHAQNLVKMIFKPDKLKKEAGTGMREGHPDKGPL"
     gene            1332..2042
                     /locus_tag="PKK85_05975"
     CDS             1332..2042
                     /locus_tag="PKK85_05975"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNS25600.1"
                     /translation="MKKYFLAIILMCVVVAASGCSMYGTGSGTLTNETKSVNGVNQVY
                     LDGIGTLVIQQGDKESLTIVAEDNIINHIESNVNGNKLELKYDSITPTPTKPVKYYLT
                     IKDLNSLSLSGAGKAESNGLKTDNLTLTIKGAGEVEMSNVDLNVLTVNIDGAGEVEMD
                     GRTNEQTVTINGAGEYDADELQSNIATITINGAGEGTLNVSKILNAFINGAGNIKYIG
                     NPQLNKHTSGVGSIKQIT"
BASE COUNT          713 a          432 c          406 g          678 t
ORIGIN      
        1 tatgaattgt ggaaactcta gaaaagttgc gcctatgcct taattccacg taaaaattga
       61 aaactggata agactcacgt ctcatccgta taaccttatt cctccagtac ttcgtaaagg
      121 atgcgcatgg ccttgctaaa tggagggtta ccagcggtta ataaaaccac ccttaagaca
      181 tctacaatct cgtccttaga aactccgaat tcctggatgg cactctgcat ttgctttttc
      241 atagctctat catcagaagc ccctgcagtt atgcccaggg ctatgagttt ctgggtacgg
      301 tagtccagga ctttaccagt gtaggttgct tcattaagag caacaagtgc atcgtagata
      361 tctggataat cctttttagc gtgatgtaca cccttaccat aaaaaacatc ttctttcatc
      421 ttattacctc cttaaatgtt cttataattc ctattattct tattttatgt tatatgtttc
      481 ttatataatt tttgtgaatt atttgtgtgc agaatatttt ttaagaccca gtggagtgga
      541 attaatggtg ggtttgtggt aaatatccta tcccattata gggggctgtc ttttcctgtc
      601 caatgaaaca ttactcagca gaattaggaa catgttaaaa taaatagata agaattaaaa
      661 gttgctataa attcatatta aagaggcccc ttatcgggat gtccttccct catacccgtc
      721 cctgcttctt tttttaattt atctggcttg aaaatcattt ttactaaatt ctgggcatgt
      781 tctcgtgccc ggttgtcagc caggaatttg aggtccttct catccttacc ctcatcttcg
      841 tggacaaaaa cctctaagat atgggtattg gtcattaatt gggcctgaat gagacccgtg
      901 gatgcctcat gggcacaaat tttatctatt ttctcaggac ccggcattcc cagagccatt
      961 accatttcac atccttcctc ttcaataagt ttcttacaag ctactggtaa atctttaaca
     1021 ccaggaactg tacgtctaat tatctttaaa ttacctatat gctgctttat ttcatccaca
     1081 gctgcagaag ccatatcata gcgtgcgaag gtagtgtcac atattccaat cttcatatct
     1141 atcacttgat tatcatctcg cctccagtag ttaatcattt tcgctaggat cttaaagccc
     1201 atcagtaatt gaacttaaga tattaccaca tcccaactat ccctttcctg atggtgaaat
     1261 ttttattaat ccctaaaaac atattattcc atatgaaaag ttgatttaat aaaaatagga
     1321 ggttcattac cttgaaaaag tattttttgg ccataatatt aatgtgtgtg gtggtggcag
     1381 catcgggatg ttccatgtat ggcacaggat caggaacgtt aaccaatgaa acaaaaagtg
     1441 taaatggagt aaatcaggtg tatttggacg ggatcggtac tttagtcatt caacaagggg
     1501 ataaggagtc cttaaccata gtggccgaag ataatatcat aaaccatata gagagcaacg
     1561 tgaatggcaa caagttggaa ctcaaatatg acagcatcac tcctacaccc accaaacctg
     1621 tgaagtacta cctaaccatt aaagatctaa acagtttatc tctttctggg gctggaaaag
     1681 cagaatccaa tggattaaaa acagacaact tgaccctgac tattaaaggt gccggtgaag
     1741 tggaaatgag taacgtggat ctaaatgtac ttaccgttaa cattgacgga gctggtgaag
     1801 tggaaatgga tggcagaaca aatgaacaga ctgttaccat taatggagca ggagaatacg
     1861 acgctgatga acttcagagt aacatcgcca ccataaccat aaatggagca ggagaaggaa
     1921 ctttaaatgt aagtaaaatc cttaacgcct ttattaatgg tgctggaaat atcaagtaca
     1981 tcgggaatcc gcaactaaac aagcatacca gtggggtagg tagtataaaa cagattacct
     2041 gagaacagtt ttttccccca tttttttctt taaataaaat ttgatttaaa aacgtttaaa
     2101 aaaaaaatag aatcagaaaa agataagctg ataaactata atacaaaaaa aattaaaaaa
     2161 agaaggtgaa gttggattta aaatccaaag gacttttcta aaagctctat gttcacgtaa
     2221 ccttcccgg
//
LOCUS       DAOQVW010000254         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_61294_length_2221_cov_2.686981, whole genome shotgun sequence.
ACCESSION   DAOQVW010000254 DAOQVW010000000
VERSION     DAOQVW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61294_length_2221_cov_2.686981"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PKK85_05980"
     CDS             <1..928
                     /locus_tag="PKK85_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNS25601.1"
                     /translation="DEGLYCCTSCGKCGEVCPKEINTFGGAIEKLREIACQEGVGPLP
                     AHRSVYELIKNTGRSVEPLGEGFIKAVSAESSEKTPKVAFFTGCLVDYRLPEIGFALI
                     EVLKEHGVDIVIPEDQVCCGSPMIRTGQTDILEDLVKRNAKALQSYDTIITVCAGCGA
                     TLKNDYPQYGVKLNVVDISEFLANRLDTSKMKPLNLRVTYHDPCHLVRGQKIKYEPRE
                     ILRKIPGVEFVEMKEPDRCCGAGGGVRSGKPEIAAALAKHKVEMIRELGVDAVITICP
                     FCENNLRLSLEEAGMNVEVMNILKLLQMAYQN"
     gene            941..1642
                     /locus_tag="PKK85_05985"
     CDS             941..1642
                     /locus_tag="PKK85_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNS25602.1"
                     /translation="MVDVVFVEPEAPGNVGFMARSMKNFGLHRLFLINPCELGEKALY
                     HAMHAQDILLNSVTYPSLEEFLVKEQVDFVVGTTGTAGGSYNVSRIAITPEKLSESLD
                     PHARVALIFGREGDGLSNQELEWCDVVVTIPTSEDYPVMNISHAAAVIFYELYKRVKT
                     YPREGVRESSRQERLDIMDYLDEILVELNYPRHKHQTAHTIFSRILGRAFITGREAHT
                     TKGIFRKIRDRLIKD"
     gene            1670..>2221
                     /locus_tag="PKK85_05990"
     CDS             1670..>2221
                     /locus_tag="PKK85_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1512 family protein"
                     /protein_id="HNS25603.1"
                     /translation="MLFNVGPLEIIGIAVAILFILLLPRIIRMRLVSSVVQATSELEA
                     MVNSSQELLVRLCQEKGNPQGDTAKLVENYLEFFMIPPVDLDPHGIMEKYRMILDLSD
                     DHFQRMAWELAPGADQEWTSNIVMTLKSILGLNGVYKMVRHNLVLAHKTGNLQALLML
                     QMNLPLIMRIVKAQFEGVHAFSEG"
BASE COUNT          568 a          518 c          611 g          524 t
ORIGIN      
        1 tgatgaagga ttatactgct gcacatcctg tggtaaatgt ggagaggtct gccccaagga
       61 gataaacacc tttggtggag ctatagaaaa gttaagggaa atcgcctgcc aggagggagt
      121 ggggcccctt ccagcgcacc gatccgtata tgaactcatt aaaaataccg ggcgttccgt
      181 ggaacccctg ggggagggat ttattaaggc agtttccgca gaatcatcgg agaaaacccc
      241 taaggtggcc ttcttcactg gttgtctggt tgattaccgc ctgcctgaga ttggttttgc
      301 cctcattgaa gtccttaagg agcatggagt ggacatcgtc atcccggagg atcaggtgtg
      361 ttgtggttct cccatgatac gtaccggaca gacggatatc ttggaagact tggttaaacg
      421 taacgctaaa gccctgcaaa gctatgatac catcataact gtctgcgctg gatgtggagc
      481 caccctaaaa aatgattacc cccagtacgg ggttaaactg aatgtggtgg acatcagtga
      541 atttttggcc aatcgcctgg acaccagtaa gatgaagccc ctgaacctgc gggtaactta
      601 ccatgacccc tgtcacttgg tccgtggcca gaagatcaag tacgaacccc gggagatact
      661 cagaaaaatt cccggagttg aatttgtgga aatgaaggag cctgatcgtt gctgtggtgc
      721 tggaggcgga gttcgttctg gaaaacccga aatagccgct gctctggcta agcacaaggt
      781 agagatgatc cgggaactgg gtgtggatgc agtcatcacc atctgcccct tctgtgagaa
      841 caacctgcgc ctctccctgg aagaagcggg aatgaatgtg gaagttatga acatcctgaa
      901 actactccag atggcctacc agaactaggg ggagcagttt atggtggatg tagtgttcgt
      961 ggagccggaa gccccgggta acgttggctt catggcacga tccatgaaaa atttcgggtt
     1021 gcaccgcctg ttcctcatta atccctgtga attaggggaa aaagccctgt atcatgccat
     1081 gcatgcccag gacatactcc taaacagtgt cacctacccc tccctggagg agtttctagt
     1141 gaaagagcag gttgattttg tggtgggcac cacgggaact gcagggggaa gttacaatgt
     1201 atcccggatt gccataaccc cagaaaaatt gtcagaatct cttgatcctc atgctagagt
     1261 ggctttgata ttcggtaggg agggggatgg gctcagtaat caggaactgg aatggtgcga
     1321 cgtggtggta accatcccca ccagtgaaga ttatcccgtc atgaacatca gccacgctgc
     1381 agcggttatc ttctatgaac tgtacaagag ggtgaagact tatccccggg agggtgtgcg
     1441 ggagtcttcc cgtcaggaaa ggttagatat tatggattac cttgacgaaa tactggtaga
     1501 actaaattat ccccggcaca aacaccagac tgcccatacc atattcagcc gaatactggg
     1561 ccgggcattc attactggcc gggaggccca caccaccaag ggcatcttcc gtaagatcag
     1621 ggaccgcctg ataaaagact gaatgaattc taagatagga gccagtagga tgctgtttaa
     1681 tgtaggaccc ctggagatca ttggaatagc agtggccata ttgttcatct tactcctacc
     1741 ccgcattatc cgcatgcgac ttgtatcctc ggtggttcag gccaccagtg aattggaggc
     1801 catggttaat agtagccagg agttgctggt cagattatgc caggagaagg gtaaccccca
     1861 gggggatacc gctaaactgg ttgagaacta tttggagttt ttcatgatcc ccccggtgga
     1921 tctggatccc catggcatca tggagaagta ccggatgata ctggatctga gtgatgacca
     1981 tttccagagg atggcctggg aactggcccc cggggcggat caggagtgga catccaacat
     2041 tgtaatgacc ctgaaatcta tcctgggcct aaatggagtg tataagatgg tccggcacaa
     2101 cctggtgctg gcccataaaa ccggtaacct gcaggcactc ctcatgctgc agatgaatct
     2161 acccctgatt atgcggattg ttaaggccca gtttgagggt gtccatgcct tctcagaggg
     2221 c
//
LOCUS       DAOQVW010000255         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_61375_length_2219_cov_2.961183, whole genome shotgun sequence.
ACCESSION   DAOQVW010000255 DAOQVW010000000
VERSION     DAOQVW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61375_length_2219_cov_2.961183"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="PKK85_05995"
     CDS             <1..1196
                     /locus_tag="PKK85_05995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS25604.1"
                     /translation="DNMKIGYFVSHYLYPDRVDQPGYADEYAHGGTEIAAYHLAQEMQ
                     RRGHQVEVFTTALDSRSVVELDKVNIHRYATQFRVASANFSWGLLRGPQEVKVDLAHA
                     HYNMPYADYSALLYKQKRGVPLVVTYHADAPETGGSFLRNQLQSFYNRHLLLRVLEEA
                     DIIITTSESYIEDSRHLKKYREKIRVIANGINLEEFQSTLSRGECRDRLGLPHHKVII
                     LFFGNLVPYKGPEVLLKAFSEVRKDHPEVLLFYAGRGPLKDGLQSLARELELEDEVVF
                     AGYVDDQDKAICYRAADIFCLPSVNRAEAFGIVNLEAMASGLPIVASRLGGIPDLVRE
                     GENGILFEPGEVDELAQVLGYMIDNPEERGKMGRAGKKMAEDYSWAKIAQKTEEVYQE
                     LLEGE"
     gene            1198..>2219
                     /locus_tag="PKK85_06000"
     CDS             1198..>2219
                     /locus_tag="PKK85_06000"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNS25605.1"
                     /translation="MEVDYICGERVRRLYGVARYEQEINKRITGVQFNRIDYQPLWRK
                     FAPEPDRVSARDKKEGRVQPPHPLTLKMAGLVRSTLQELDGYRYQHRVQGNIKKDNIK
                     HLTSQELGYLLHHIPPEKTIINCHDLIPWVYDQDRSQRWKSNLMGMKRAGKIITVSQF
                     SKEEITSHLDYPEEDIQVIPDAVDHAHFHPQVKPLNRTRWGGVESDRYLLYVGSETPR
                     MNLEVLLEALAKLKNLIPGIKLLKIGEAQSYGAREILLKKVKDLELEKDVIFLGYVAE
                     EDLPGFYRTAEVLVYPCLYAGFGLPPLEAMASGTPVVTSNTTSLPEVVGDAGLMFNPQ
                     GVDELVT"
BASE COUNT          552 a          586 c          663 g          418 t
ORIGIN      
        1 aggataacat gaagatcggt tacttcgtaa gccactacct ctacccggac cgggtggacc
       61 agccaggata tgcagatgaa tatgcccatg gaggtactga gatcgccgcc taccacctgg
      121 cccaggagat gcagagaagg ggccaccagg tggaggtgtt caccactgcc ctggacagtc
      181 ggagtgtggt ggaattggat aaggtgaaca tccaccgcta cgccacccag ttccgggtgg
      241 ccagtgccaa cttcagctgg ggactcctcc ggggacccca agaggtaaag gtggacctgg
      301 cccacgccca ctacaacatg ccctacgccg actactcggc ccttctctac aaacagaaga
      361 ggggggtgcc cctggtggtg acctaccacg ctgatgcccc ggagaccggg gggagttttt
      421 taagaaacca gctgcagagc ttctacaacc gccacctgct gctcagggtg ctggaggaag
      481 cggatataat aattaccacc tccgagtcct acatcgaaga ttcccgccat ctgaaaaaat
      541 accgggagaa gatccgggtc atagccaacg gcatcaacct ggaggaattc cagtccacac
      601 tctctagggg ggagtgcagg gatcgtcttg gtttaccaca ccataaggtg atcatactct
      661 ttttcgggaa cttagtaccc tataaagggc cggaggtgct cttgaaggcc ttcagtgagg
      721 taagaaagga tcaccctgag gttttgctat tctacgcggg gcggggaccc ctgaaggatg
      781 gtctacagag tctggcccgg gagttagagt tggaggatga ggtggtcttc gcgggttatg
      841 tggatgacca ggataaggcc atctgctacc gggctgccga tatcttctgt ctgccctcgg
      901 tgaaccgggc cgaggccttc ggcatcgtca acctggaggc catggccagt ggcctgccca
      961 ttgtagccag ccgcctgggg gggatcccgg atctggtccg ggagggagag aacggtattc
     1021 tttttgaacc cggagaggtg gatgaactgg cccaggtcct aggctacatg atagataacc
     1081 ctgaagaaag ggggaaaatg ggccgtgccg ggaaaaagat ggcagaagac tatagctggg
     1141 caaagatcgc ccagaagacc gaggaggtct accaggaact ccttgagggt gagtaagatg
     1201 gaagtggact acatctgcgg ggaacgggtt agacggttgt acggagtggc ccgctacgag
     1261 caggagataa ataaaaggat cacaggggtg cagtttaacc ggatcgacta ccagcccctg
     1321 tggaggaagt tcgccccgga accggataga gtctcggccc gggataaaaa agaaggcaga
     1381 gttcagccac cccaccccct caccctgaag atggccggtc tggttagaag caccctgcag
     1441 gaactggacg gctaccgcta ccagcaccgg gtccagggaa atattaaaaa ggataacatc
     1501 aagcatttga cctcccagga gctgggatat ctcctccacc atatcccccc agaaaagacc
     1561 atcatcaact gccacgacct cataccctgg gtctacgacc aggaccgcag ccagcggtgg
     1621 aagagtaacc tgatgggcat gaagagggct ggtaagatca taaccgtgtc ccaattttct
     1681 aaagaggaga taaccagcca cctggattat ccggaggagg atatccaggt catcccggat
     1741 gccgtggacc atgcacactt ccacccccag gtgaagcccc tgaaccggac ccgctgggga
     1801 ggagttgaaa gtgaccgcta cctgctctac gtgggcagtg aaacaccccg gatgaacttg
     1861 gaagtgttat tggaggccct ggccaagtta aagaacctga tcccagggat taagttactg
     1921 aaaattggtg aagcccagag ctacggggcc cgggaaattc ttctaaaaaa ggtaaaggac
     1981 ctggaactgg aaaaggatgt tatattcctg ggctacgtgg ccgaggagga tctgcccgga
     2041 ttttaccgaa ccgcagaggt cctggtctat ccctgcttat acgccgggtt tggactgcca
     2101 cccctggagg ccatggccag tggcacccca gtggtcacct ccaacaccac cagcctgccc
     2161 gaggtggtgg gggatgcggg tttgatgttc aacccccagg gagtggatga actggtcac
//
LOCUS       DAOQVW010000256         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_61444_length_2217_cov_3.179001, whole genome shotgun sequence.
ACCESSION   DAOQVW010000256 DAOQVW010000000
VERSION     DAOQVW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61444_length_2217_cov_3.179001"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKK85_06005"
     CDS             <1..248
                     /locus_tag="PKK85_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNS25606.1"
                     /translation="NADTIIVLMGIGNLEENVQEILKYKDPETPVCIIENGTMPGQRI
                     VTGALGDIAQKNVQPPALVIIGPVVDVYNQMKNSGSN"
     gene            257..1048
                     /locus_tag="PKK85_06010"
     CDS             257..1048
                     /locus_tag="PKK85_06010"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNS25607.1"
                     /translation="MKRLKGKVIAVTRPQERAQVAADIITNEGGTAWLAPTLELKMTR
                     TKSLMKLCQHAPNLDWVILTSPASLESLFHYCPDFKHHLNDDCRIAVIGPRTQRALEG
                     YGIKAELVPEDYTAEGLLKMMAPLDLEGKIIGIPRTFSARDVLPEGLRKLGAQVLLAE
                     AYKSTVPRDIKPVQDLIEGIFNEEVDAVTFTSPLTVHNLFKIAEDSENQLIDFLSSEK
                     IMLVAIGPITGDAIQEYGLTYIAPSKYTVEAMLDIMIEEMDEDRL"
     gene            1074..1373
                     /locus_tag="PKK85_06015"
     CDS             1074..1373
                     /locus_tag="PKK85_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein Srp19"
                     /protein_id="HNS25608.1"
                     /translation="MADKDRTMIWPAYLDSKKSQREGRKIPINQAVSTPKIREIKQAA
                     VKLGLNPYTDKGKSYPKSWWENSGRVIVDRKFSKRELILKISNLIKSSRSDKKHK"
     gene            1458..>2217
                     /locus_tag="PKK85_06020"
     CDS             1458..>2217
                     /locus_tag="PKK85_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNS25609.1"
                     /translation="MHKAHELVKNSEDIKILTHIDCDGISAGAILSSTLDRLGVDHEV
                     EFISLDMIPGLKSENELTIFSDLGSGQNLDHFTTSNSKVLILDHHPPLRKMGSTWKGE
                     FIEINPYYFDIDGSSQVSGGGLSYLLAKKFGFEDLSWIGVLSAVGDMQNSLTGRLEGL
                     NTYILKDGVQRGLVRTFNDLAIYGRQTRPLYIALSYFGDVKLPISNNKNECIELLKKL
                     DIARKKGKVPRTLSDLNENEKEKLFSELVKMLTRE"
BASE COUNT          721 a          439 c          510 g          547 t
ORIGIN      
        1 ttaacgccga caccatcatc gtacttatgg gtattggtaa tctggaagaa aatgtgcagg
       61 agatcctgaa gtacaaagat ccagaaacac cggtgtgtat catagaaaat ggcaccatgc
      121 ctggccagag aatagtgaca ggagcactgg gagatatagc ccaaaaaaat gttcaacccc
      181 cagctctggt gataatcggc cctgtggtgg atgtttacaa tcaaatgaaa aacagtggta
      241 gtaattgaag atggccatga aaagattgaa agggaaggtg attgccgtca ccaggcccca
      301 ggagagggcg caagtagcag cagatattat caccaatgag ggaggtactg cctggctggc
      361 acccacactg gaactgaaaa tgacccgcac aaaatcattg atgaaactgt gccagcatgc
      421 cccgaacctg gactgggtta tattgacctc tccagcctca ttggaatcat tgtttcatta
      481 ctgcccggat tttaaacatc atttaaatga tgattgtagg atcgctgtta ttggaccccg
      541 cacccaaagg gctttggaag gatacggaat aaaagcggag ttagtgcctg aagattacac
      601 ggcagaaggt ttgttgaaga tgatggcccc cttggatctg gaaggaaaga tcataggtat
      661 cccccggaca ttttcggccc gtgacgtcct gccagagggc ttgagaaagc tgggtgccca
      721 ggtattactg gctgaagcat ataaatccac cgtgccgcgg gacattaaac ccgtgcaaga
      781 tttaattgaa ggcatattta acgaagaagt agacgctgtt acgttcacta gccctttaac
      841 cgtgcataac ctcttcaaaa tcgctgagga tagtgaaaac cagcttatag attttttaag
      901 ctctgaaaaa atcatgctgg tagccattgg accaataact ggggatgcca ttcaggaata
      961 tggtttaacc tacattgctc cctcaaaata cacagtagag gccatgttgg atataatgat
     1021 agaagagatg gatgaagata gactttaaga gataggttaa gagatggatt agtatggcag
     1081 ataaagatag aaccatgatc tggccggctt atctggactc gaaaaagtcc cagagggaag
     1141 ggagaaaaat tcccataaac caagcagttt caacacctaa aattcgtgaa atcaagcagg
     1201 ccgcagttaa actgggttta aatccctata ctgataaagg aaaatcatat ccaaagtcat
     1261 ggtgggaaaa ctctggaagg gtgattgtgg atagaaaatt ttccaaacgc gaattgattc
     1321 tgaagatcag taacctcatc aaatcgtcca gatcggataa aaaacataaa taaggtgatt
     1381 ttttacaaac taatctctta aaatttgtta ataaaaaaaa tatccaggga aatattccac
     1441 agccactgga tcagcccatg cataaggccc atgaattggt gaagaattct gaagacatca
     1501 aaattttaac tcacatcgat tgtgatggta tatccgcagg ggcaattcta tcctccaccc
     1561 tggaccgcct aggagtagat catgaagtag aatttatcag tctggatatg atccccgggc
     1621 tgaagtctga aaacgagttg accatatttt ctgacctggg ctctggacag aatctggacc
     1681 attttaccac ttccaactcc aaagttttaa tcctagacca tcacccccct ttaagaaaga
     1741 tgggatcaac ctggaagggc gagttcattg aaataaatcc ctactacttt gatatagacg
     1801 gatccagcca ggtctcgggt gggggcttgt cctacttgct ggcaaaaaaa ttcggcttcg
     1861 aggatctgag ctggatcgga gttctgagtg ccgtggggga tatgcaaaat agtttaactg
     1921 gaagattaga aggattaaat acgtatatac taaaagatgg tgttcaaagg ggtctggttc
     1981 gaacttttaa tgatctggca atttatggcc ggcaaactcg ccccctatac attgctttat
     2041 cctattttgg agatgttaaa ctccccataa gtaataacaa aaatgaatgc atcgaactac
     2101 tcaaaaaact cgacatagcc cgtaaaaaag gtaaggtacc ccgaacttta tctgatttaa
     2161 atgaaaatga gaaagaaaaa ctgttctcag agttagtaaa gatgttgaca cgagaag
//
LOCUS       DAOQVW010000257         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_61445_length_2217_cov_3.164662, whole genome shotgun sequence.
ACCESSION   DAOQVW010000257 DAOQVW010000000
VERSION     DAOQVW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61445_length_2217_cov_3.164662"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PKK85_06025"
     CDS             complement(<1..588)
                     /locus_tag="PKK85_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS25610.1"
                     /translation="MKFVAIASKGGKELLRDHRGFIMILIFPLLFMLVFGFAFGGMGQ
                     ANQPHVIAVINYDQGATLPISGERLDLGSNFTHVLEDVKYEDSEVKIFVVRGTDLNEA
                     NSLLRQKTVDAVVIIPENFTESILDSTGGGTTPTPVVIRGDTGYMGFGVSQGILVGII
                     EQYQDELSAQLSSQFTGKTVQPVPKLIETRVESIPG"
     gene            complement(595..1536)
                     /locus_tag="PKK85_06030"
     CDS             complement(595..1536)
                     /locus_tag="PKK85_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS25611.1"
                     /translation="MKDYIIEARGLTKDFGEFRAVDKLDLKIERGEVFGFLGPNGAGK
                     TTSINMMVGLLQPTSGEILIDGQEVNRVEKGVIGICPQELVLWDSLTPLESLKLMGIM
                     YDIPPKTRQRRAEKLLSDLLLTDKANTQVKDLSGGMKRRLNLALAIMHQPEIVVLDEP
                     SEGLDPQSRRVLWSYIRSLRDDEGKTVILTTHLMDEADKLSDRIAIMDHGKLLRLDTP
                     SNLKKEIGEGDVVDMKLSNPKMNQTVIDALTPMKNIDMAVQMDERINIRALNAVAQLP
                     TLMEEIEGIGVKIVDLSVRQNTLEDVFIELTGSTLRE"
     gene            complement(1662..>2217)
                     /locus_tag="PKK85_06035"
     CDS             complement(1662..>2217)
                     /locus_tag="PKK85_06035"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HNS25612.1"
                     /translation="EKYSNQWVERASYLIFLNKTCYNGLFRQNSKDEFNVPFGKYKNP
                     RICDEKNIIQVNKALEDTEIIMGDFSNSEGYIKKNTLVYLDPPYRPISPSSSFTCYAK
                     EGFDDEDQIRLSKFFHRMDKRGAYLMLSNSDPKNVDANDEFFDELYQEYFLERVPAKR
                     SINCDASKRGEIKELIVRNYCENG"
BASE COUNT          522 a          526 c          465 g          704 t
ORIGIN      
        1 gcctggtatg gattctaccc gggtttcaat gagcttaggc acaggttgaa ctgttttccc
       61 tgtgaattgg ctgcttagct gggctgataa ctcgtcctgg tactgctcta tgattcccac
      121 cagtataccc tgggaaactc caaatcccat gtaccccgtg tctcccctta tgacaactgg
      181 ggtgggggtt gttcctccac cagtggagtc tagtatggat tctgtgaagt tttcggggat
      241 tataactacg gcatccacgg ttttctgtct gagaagggaa tttgcctcgt tcagatcagt
      301 ccctctgact acgaagatct taacctcact atcctcgtac ttaacatcct ccaatacatg
      361 ggtgaagttg cttcccaaat ccagcctttc accagaaatg gggagggtgg ctccctggtc
      421 atagttaatt accgcaataa catggggctg gttggcttgg cccatacctc caaaggcgaa
      481 gccgaaaacc agcatgaata gtaaggggaa gatcaggatc atgatgaatc cacggtggtc
      541 cctcaggagt tccttccccc ctttagatgc tattgccacg aatttcatcg tatcctactc
      601 ccgcaaggtg ctgccggtga gttcgatgaa cacatcttcc aatgtattct ggcgcactga
      661 taaatccacg attttcacgc ctatcccctc gatttcttcc atcaaggtag gtaactgggc
      721 caccgcattc agggccctga tatttattcg ttcatccatc tgaaccgcca tgtctatgtt
      781 cttcatgggg gtcagggcat ctataactgt ctggttcatt ttaggattag atagtttcat
      841 gtccaccaca tctccctcac cgatctcctt tttcaggttg ctgggggtgt ccaggcggag
      901 cagtttacca tggtccatga tggcaatgcg gtccgatagt ttatcagcct cgtccatgag
      961 gtgggtggtt aatatgacag ttttaccctc atcatctcgt aaggaacgta tgtagctcca
     1021 taaaacccgg cgtgactggg ggtctagtcc ctcggatggt tcgtccaaga ccacgatctc
     1081 tggctggtgc ataatggcta gtgccaggtt taaacgcctc ttcatacccc cggagagatc
     1141 tttaacttgg gtgttggctt tatctgttaa gagtaggtcg gataacaatt tctcagccct
     1201 tctttgccgg gttttggggg gtatatcgta catgatcccc atgagcttaa gactttcaag
     1261 gggagttaaa ctatcccaca gtaccagttc ctggggacag atccctatga cccccttctc
     1321 caccctattt acttcctgtc catctatgag gatttcgcca ctggtgggtt gtaacaagcc
     1381 caccatcatg ttgatggaag tggttttacc cgccccattt gggcctaaaa atccgaaaac
     1441 ttctcccctt tcaattttaa ggtcgagttt atccaccgcc cggaattcac cgaaatcttt
     1501 ggttaagcca cgagcttcaa tgatgtagtc cttcaattaa atcccctatc cagtgtcctg
     1561 ttattgcagg aataatttac agataatcgt tcattaactt attctattca tgttaaaggc
     1621 tgtaggatca gaatttttgc aggaatattt ccacatgctt tttatccatt ttcacagtag
     1681 tttcttacta ttaactcctt gatttctcct cgtttagatg catcacaatt tatggacctt
     1741 tttgctggaa ctctttctag gaagtattcc tggtatagtt catcaaaaaa ttcgtcgttt
     1801 gcatcgacat tttttggatc agagttgctt aacatcaaat atgcaccacg tttatccatt
     1861 ctatggaaga atttggatag acgtatctga tcctcatcat caaagccttc tttagcatag
     1921 caggtgaagc tggaggatgg actgatgggg cggtagggtg ggtccaggta caccaaagta
     1981 ttttttttta tgtatccctc tgaattacta aaatctccca tgataatttc agtgtcctct
     2041 agagccttgt ttacttgaat tatgttcttt tcatcacata ttcttggatt tttatacttc
     2101 ccaaatggaa cgttaaattc gtccttacta ttttggcgaa ataatccatt gtaacaggtt
     2161 ttatttaaaa atattaaata gctggctctt tcaacccatt ggttgctgta cttttca
//
LOCUS       DAOQVW010000258         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_61564_length_2214_cov_3.568319, whole genome shotgun sequence.
ACCESSION   DAOQVW010000258 DAOQVW010000000
VERSION     DAOQVW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61564_length_2214_cov_3.568319"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PKK85_06040"
     CDS             complement(<1..342)
                     /locus_tag="PKK85_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00288 family NYN domain-containing protein"
                     /protein_id="HNS25613.1"
                     /translation="MRQKLNSFKEYIPRVRRNSEGKTVGLLVDGPNMLRKEFGFNLDM
                     VREIIEEYGNLRVGKVFLNQYASDKLIEAVVNQGFSPIVVAGETDVHLALEAFELIHN
                     PNIDIIAIMTRD"
     gene            complement(560..1018)
                     /locus_tag="PKK85_06045"
     CDS             complement(560..1018)
                     /locus_tag="PKK85_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNS25614.1"
                     /translation="MKVEEKRVEETKVAYIPYRGSYDKIPVLMLKVGQWIADQGLEMT
                     GLVYGTYYNTPEEVAEKDLQYEIGFSFAGEGYQDANVGVKEIPAHTVLAAMHQGPYTG
                     MGPVIHAVVDYAVNHGYDIVGPITEVYLNDPHRTPEEELLTEVHLPVIKK"
     gene            complement(1925..>2214)
                     /locus_tag="PKK85_06050"
     CDS             complement(1925..>2214)
                     /locus_tag="PKK85_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNS25615.1"
                     /translation="VPEEELTWEVGAAFLGELEGEGDIQVKKVPEHEVVSTIFKGPYS
                     ETHAVYGELYDHAQKNGHRINGPQLESYPNHPNLLPESELLTEVQFPVLKQ"
BASE COUNT          674 a          468 c          424 g          648 t
ORIGIN      
        1 atcacgagtc attatagcta tgatgtctat gttggggttg tgtataagtt caaaggcctc
       61 caatgccaga tggacgtcag tttctccagc caccactatg ggtgaaaatc cctgattaac
      121 cacggcttct ataagtttat ctgaagcata ttggttcaaa aagaccttac caactcttaa
      181 attaccatat tcttctatta tttcacgaac catatccaga ttaaagccga actccttccg
      241 aagcatgtta ggaccatcaa ctagaagtcc caccgttttt ccttcagaat ttctcctgac
      301 tcggggaata tattctttga atgaatttaa tttttgacgc attttctgtc tccagtataa
      361 taaagcgcag gtaataaaga aagtaataaa tattgatttt gatatcctga actctaagat
      421 tgttatattt atctataata aacattatta aatctttatg ccaaaattac acccaaatct
      481 atcgatagtt gtacccgcaa aaaggatata aatacctttt ttcggaaaat cagggtagta
      541 tgggggaata ataaaaattt tatttcttga taaccggcag atgaacctca gttaagagtt
      601 cttcctcagg agtgcggtgg ggatcattga gataaacctc ggtgatggga ccaacaatat
      661 cgtaaccatg gttaactgca tagtctacca cagcatgtat aaccggaccc atccctgtgt
      721 aaggtccctg atgcatagcc gccagtacgg tatgggctgg tatttctttc actcccacgt
      781 tagcatcctg atatccttca ccagcaaatg aaaatccaat ctcatactgc agatctttct
      841 cagccacctc ctcaggggtg ttgtagtagg tcccatatac caagccagtc atttccagcc
      901 cctgatccgc tatccattgg cctactttca gcatgagtac tggaatctta tcgtaactcc
      961 ctctgtaagg gatataagca actttagtct cttctaccct cttctcctct actttcatga
     1021 taattcctct gaacatggtt tgttttatag ggtaaaaaat ttaccccatg ttgttatgtc
     1081 tgggtggtta tgaaaatcag caggagtacc agacagatct aaagtcttcc tatccacacg
     1141 tcatgaatat ggaaactacc ttggccagtg ttggagataa tggcatctaa aggcccacaa
     1201 agcacaagtt actcaagact gacgcggata cttcgaagat tctttagaaa aatcgttgca
     1261 ggagaaaagt aagatgataa tactatccac taacatcaat aataggttaa tagtaggcac
     1321 ctgccactct gctgacatca tcgttagctc ctccaccaga agttgatggt gtaggaaact
     1381 ccatccaagt tggtgtaaat gctaaaccag tggtggtgaa ccagggcatg gccttaaagt
     1441 agggattgag ataggaaatt ctccagaaag ataatagggt agaacacccc taaagaacca
     1501 gataatggtg gaaaaaaacc atggccacct taaagaatgg tggtttctct ctaaagactt
     1561 taagataaag cacagaaaag atcatggtta ccttcagaaa atagaaaggg agttgaatac
     1621 tgatattaat gataaattaa gggcataata agggatatta accccagaaa gaggcacacc
     1681 ccaccgttgt agtgcatgat ggttttaatc tcatgcctgc ttaacctagc catctgcgcg
     1741 ttagtaactc cccctaaata ttttgatagc aacttattca actatttctg gatcaaggag
     1801 gatcggtcat tgtttaattt tcaatcaaat tcttgacaat tactaaatgt aatataagtt
     1861 tttgttgtga tgaagttttg aattaaaaaa acagttcaga cctaaaaata agaaaattag
     1921 gagtttactg cttcaaaacc gggaactgaa cttcagtaag cagttctgac tcgggaagga
     1981 gattagggtg gttgggataa ctttccagtt gaggcccatt tatcctgtgt ccgtttttct
     2041 gggcgtggtc gtaaagctca ccatacactg catgggtttc gctgtatgga cccttaaata
     2101 tggttgaaac cacttcatgc tccgggacct tcttgacttg gatatcaccc tcaccctcca
     2161 gttctcccag gaatgcagca cctacttccc aagtcaactc ttcctcaggg actt
//
LOCUS       DAOQVW010000259         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_61986_length_2205_cov_3.356279, whole genome shotgun sequence.
ACCESSION   DAOQVW010000259 DAOQVW010000000
VERSION     DAOQVW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61986_length_2205_cov_3.356279"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="dapF"
                     /locus_tag="PKK85_06055"
     CDS             <1..627
                     /gene="dapF"
                     /locus_tag="PKK85_06055"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNS25616.1"
                     /translation="RCFAKFIYENKVVEEETLEVETLGGIKTLELNVVEGKVVSTRVD
                     MGPASFFAREIPMNTDKEEFLDGELDVGGMKFTLTTVNVGNPHAVIFTDDLEEVDLGE
                     VGPLIECHPAFPERINVHFVRIISPGEVEMITWERGAGFTLACGTGATSVALAGYRLG
                     HLDRDVQVHLPGGDLKISVYHGEGKLGAFMEGGATMVFDGIMEIDLGP"
     gene            complement(698..1759)
                     /gene="cobJ"
                     /locus_tag="PKK85_06060"
     CDS             complement(698..1759)
                     /gene="cobJ"
                     /locus_tag="PKK85_06060"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HNS25617.1"
                     /translation="MIYVVGIGPRRDDMTIRAFKVLQDADVVVGYGGYLRQIRDLTED
                     KEVIARGMGQEVERAELAIFHSRQGKKVALVSSGDPGVYGMANVLYQVLGKYEDVKVE
                     VVPGVTAATYTSALLGAPLHDFAVISLSDILTPLSEIKRKVKKAAEADLIIVFYNPRS
                     KRRKIPLKEAYQILKEVRKATTLVGMVKTGDDGPEVTVTTLEDLSLENVDMSTTLLVG
                     NTFTYLQDGKMVTPRGYLSPHTTHPLASDFYEKYLAGEGVEGPNLSCEYYPCHQHPQN
                     CTFCYCPFYPCGDSSTGGHWIKEKQVWSCEGCQWIHQDDVVQCIRAKLDQILHNIGDL
                     EDRKKELLKLRRECVFSTR"
     gene            complement(1813..>2205)
                     /locus_tag="PKK85_06065"
     CDS             complement(1813..>2205)
                     /locus_tag="PKK85_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25618.1"
                     /translation="DAISSIFNFGGNSLDNKKALVILNKEEVVEALKTKAGMDGINTN
                     TLVAYHVQNNDNNTILVNITAMLYKDVQIGGGSKNSTRGQIVISPQETYSVTATAIAT
                     KKNTGITVDVDSIQVTSTRKLYNTNKTS"
BASE COUNT          551 a          480 c          532 g          642 t
ORIGIN      
        1 cgctgttttg ccaaattcat ctacgagaac aaggtggtgg aggaggaaac cctggaggtg
       61 gaaaccctgg gcggaattaa aaccctggag ttaaatgtgg ttgagggtaa ggtggtttcc
      121 actagggtgg atatgggtcc agccagtttt tttgccaggg aaatacctat gaacactgat
      181 aaagaggaat ttcttgatgg agagttggat gttggtggaa tgaagttcac ccttaccaca
      241 gtgaatgtgg gcaaccccca tgctgtgata ttcacagatg atttggaaga ggtggacttg
      301 ggagaggttg ggccgcttat tgaatgtcac cctgcattcc cagaacgtat aaatgtgcat
      361 tttgtgcgca tcatcagccc aggtgaggtg gaaatgataa cttgggagag gggggctggt
      421 ttcaccctgg cctgtggcac aggagctact tcagtggctc tggcaggata taggttggga
      481 catctggacc gggatgtgca ggtgcattta ccaggtggag acctgaaaat tagtgtttat
      541 catggagaag gtaagttagg ggcatttatg gaaggcgggg ccaccatggt atttgatgga
      601 ataatggaga tcgacttagg cccctgaact cttttattca agataacccc ctaaacacaa
      661 acccctaatc ggtgtctttc ctttaagcat gcctttatta tctggttgag aagacgcatt
      721 cccttcgtaa ttttagtagc tctttcttac gatcctcaag gtcccctatg ttgtgcaata
      781 tctgatccag tttggcccgt atgcactgca caacatcgtc ttggtgaatc cactgacatc
      841 cttcacaact ccaaacttgc ttttccttta tccagtgccc acctgtactg gagtcaccgc
      901 atggataaaa gggacagtaa cagaaggtgc aattttgggg gtgctggtgg cagggatagt
      961 actcacagga taggttgggt ccttccaccc cctctccagc caggtatttt tcataaaaat
     1021 ctgaagccag aggatgagtg gtatgtggtg agaggtagcc gcggggagtt accattttac
     1081 catcctgaag ataggtgaag gtgttaccca ccaagagggt ggtggacatg tccacattct
     1141 ccaatgaaag atcttccagg gtagtaactg tgacttccgg accatcatca ccggttttaa
     1201 ccattccaac cagggttgtg gcctttctga cttccttcaa aatctgataa gcctctttaa
     1261 ggggaatttt tctgcgttta ctgcgggggt tgtagaaaac tatgattaaa tctgcttctg
     1321 ctgctttttt aacctttctt tttatctcag aaaggggagt tagaatgtca cttagactta
     1381 taactgcaaa atcatgtaat ggagcaccta agagtgctga ggtgtaggtt gcagctgtga
     1441 cccctggaac cacctccacc ttcacatcct catatttgcc cagaacctgg tagagcacgt
     1501 tggccatacc ataaaccccc ggatctccac tactaactag ggctaccttt tttccctgac
     1561 gcgaatggaa aatagctagt tctgcacgtt caacttcctg gcccattccc ctggctatta
     1621 cttctttatc ttcagtaaga tcacggatct gcctcagata gccaccatag ccaacaacca
     1681 catctgcgtc ctggagaact ttaaaagccc taatggtcat gtcatcacgg cgagggccta
     1741 ttccaaccac gtatatcatg atatagtctc tcttaagata gtgttaacaa taaatttaat
     1801 agtacattgg tattaactgg ttttattggt attgtagagc tttctagtac ttgtaacctg
     1861 gatgctgtcc acatcaactg tgattccggt gtttttcttg gttgcgatgg ctgtggcggt
     1921 tacactatag gtttcctggg gtgaaataac aatctgtccc ctagtgctgt tttttgagcc
     1981 accaccgatc tgcacatctt tataaagcat ggcagtaata ttaacaagga tggtgttatt
     2041 atcgttgttc tggacgtggt aggccaccag ggtgttggta ttaataccat ccatccctgc
     2101 cttggtttta agagcttcca caacctcttc tttgttcagg atcaccaggg cttttttatt
     2161 gtccagggag ttaccaccaa aattaaaaat ggatgatatg gcgtc
//
LOCUS       DAOQVW010000260         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_62613_length_2191_cov_4.229869, whole genome shotgun sequence.
ACCESSION   DAOQVW010000260 DAOQVW010000000
VERSION     DAOQVW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62613_length_2191_cov_4.229869"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /gene="pscS"
                     /locus_tag="PKK85_06070"
     CDS             complement(<1..1112)
                     /gene="pscS"
                     /locus_tag="PKK85_06070"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HNS25619.1"
                     /translation="MECQDYALNRTLERENLNLNPLQRGGFLPLEARKALYEFSDGYS
                     VCDYCSGRLDQVKKPSVAGFLDDLAQFLNVDAVRTVHGAREGKFAIMHAICQPGDTIL
                     VDGNAHYTTHLAAERNQLKLVEVPTSGHPEYKVEPESYREVLNQLHDQCEEPSLLVLT
                     HVDGDYGNLTDSQGVGKIASEYGIPLLLNCAYSMGRMPIDAKKLKADFVVGSGHKSMA
                     ASGPIGVLGLKEEWADKVFKRSSTHSVKEVEMLGCTSRGAPLATLMASFPHIMERVGK
                     WNQEVEKTRKFVAQMEKIGGISQIGVKPTDHDLVRFETSLFHQIAEKHPRRGFFLYEE
                     LKKRNIVGIKRGQTKWFKCSVYGFNSQQVEYITRSF"
     gene            complement(1278..>2191)
                     /locus_tag="PKK85_06075"
     CDS             complement(1278..>2191)
                     /locus_tag="PKK85_06075"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphorylating glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNS25620.1"
                     /translation="RTPDFEAQMAVEKGYPLYISAPGREGQFEEAGIKVAGTIDDLYD
                     MVDLMVDCTPEGIGAQNMEVYKKKGVKGIFQGGEKQENIGHSFNSFTNYQDAWGSDYV
                     RVVSCNTTGLCRTLKPIHDLCGIKKVRAVMVRRGADPGQVKKGPINAIVPNPPTVPSH
                     HGPDVQTVMYDLNITTMALLVPTTLMHQHNLMVELDSVPSVDEVVDLLEVTPRVMLFE
                     ANRGAGSTAEVMEFARDMGRPRSDLFEIAVWKESININDGELFYMQAIHQESDVVPEN
                     VDCIRAMLEMEEDAEKSIAKTNQAMGI"
BASE COUNT          557 a          561 c          456 g          617 t
ORIGIN      
        1 tggaagctac gggtgatgta ctctacttgc tgggagttga agccgtaaac gctgcatttg
       61 aaccacttgg tctgcccccg tttaattccc actatattcc gctttttaag ttcttcatag
      121 agaaaaaaac ctcttctagg atgtttttca gcaatttggt ggaataatga agtttcaaaa
      181 cgcaccaagt catgatcagt aggtttgact ccaatctgac ttattccgcc tatcttttcc
      241 atctgggcca caaacttccg ggtcttctca acttcttggt tccacttccc caccctttcc
      301 attatgtgag gaaaggaggc catgagagtg gctagagggg ctcccctgct ggtacacccc
      361 agcatttcca cttccttcac agaatgagtg gaagaccttt tgaacacctt atctgcccat
      421 tcttccttca agcccagaac gccaataggt cccgaagcag ccatgctctt atgaccactt
      481 cctactacga aatcggcttt cagcttttta gcatcaatgg gcatacgtcc catggaataa
      541 gcacagttaa gtagaagagg aattccatat tcactggcaa tctttccaac cccctgggaa
      601 tcagttaaat taccataatc tccatcaaca tgggtcagaa caagcaagct gggctcttca
      661 cactggtcat gcaactgatt tagcacttcc cggtaacttt caggttccac tttgtactca
      721 ggatgtccac tggtgggcac ctcaaccagc ttaagttggt tgcgctcagc tgccagatgg
      781 gtggtgtaat gggcattccc atccaccaga atggtatctc ctggctggca gatggcatgc
      841 atgatggcaa acttgccttc ccgggccccg tgaaccgttc gcactgcgtc cacatttaag
      901 aactgggcca gatcatccag aaaaccagcc acggaaggtt tttttacctg atccaaccgg
      961 ccagaacagt agtcacagac actgtatcca tcggaaaact catacaaagc tttccttgct
     1021 tctaatggta aaaaaccgcc tctttgcagg ggattcaaat ttaaattctc tctttccaga
     1081 gttctgttga gcgcgtaatc ctgacattcc atggtaacac ctaaaggatg atatctaaag
     1141 acttaactac aaatttggat ttaatcaaca tatcttatga atggatggtg actatttttt
     1201 tactagaact tttcaaggaa aatttgaata aaattacttt ttaaagatta aaaaaaatag
     1261 ctccggggaa tttttattta aattcccatg gcctgattgg ttttggcaat tgatttctca
     1321 gcatcctctt ccatttccaa catggcccgg atacaatcca cattctcggg tactacatct
     1381 gattcctggt ggatagcttg catgtaaaat agttcaccat cgttgatgtt tatagactcc
     1441 ttccacacgg caatttcgaa aaggtcgcta cgaggcctac ccatgtcccg ggcgaactcc
     1501 ataacttcag cagtggatcc ggcacctcta ttggcctcaa agagcataac ccggggagta
     1561 acttccagga ggtcaacaac ttcatccaca gatggcaccg agtccaactc aaccatgagg
     1621 ttgtgctggt gcatgagggt ggtgggaacc agcagggcca tggtggttat attcaagtcg
     1681 tacataaccg tctgcacatc tgggccatgg tgactaggca ctgtaggtgg gttaggtaca
     1741 atagcgttta taggtccttt tttgacttgt cccgggtcag cgcctctacg aaccattacc
     1801 gctcgtacct ttttaatacc gcagaggtca tggattggtt ttaaggtccg acacagacca
     1861 gtggtgttac aggaaactac ccggacataa tctgatcccc acgcgtcttg gtagtttgtg
     1921 aaggagttaa atgaatggcc aatgttttcc tgcttttctc caccctggaa aatgccctta
     1981 acacccttct ttttgtaaac ttccatgttc tgggcaccga taccctccgg ggtgcagtcc
     2041 accatcagat ccaccatatc gtacagatca tcgatagtcc cggccacttt aattcctgct
     2101 tcttcaaact gcccctccct tcccggagcg ctgatgtaaa gggggtaacc tttttcaacc
     2161 gccatctggg cttcaaagtc aggggttctc t
//
LOCUS       DAOQVW010000261         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_62786_length_2187_cov_3.392120, whole genome shotgun sequence.
ACCESSION   DAOQVW010000261 DAOQVW010000000
VERSION     DAOQVW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62786_length_2187_cov_3.392120"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="PKK85_06080"
     CDS             complement(<1..120)
                     /locus_tag="PKK85_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNS25621.1"
                     /translation="MKRVNVGVIGVGAMGHNHVRVYSRLKNANLMAVSDLMKGT"
     gene            complement(117..989)
                     /gene="hemC"
                     /locus_tag="PKK85_06085"
     CDS             complement(117..989)
                     /gene="hemC"
                     /locus_tag="PKK85_06085"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNS25622.1"
                     /translation="MKVGTRGSGLALAQTKGVIADLSRLTSEKIEIKVIKTTGDKIKN
                     SQLYNMDTKGIFTRELDKALLEEEVDFAVHSLKDLPTDLNEDLEIVAVPPRETPRDAL
                     ISAYPWDELPENASLGTSSLRREAFCKFHQKKVIIKPIRGNIDTRVRKVENGQYDATL
                     MAAAGLKRLGLAHHIQQTFPIDYITPAAGQGALAIVSRKDSSVKEVLKGLSDFISYQE
                     VTAEKRVLQDLGVGCQWPLGVCARADGDLLDLYGALLTKEGEVISRVSVRGPLKKAEK
                     VGKEAARIMGDAYS"
     gene            complement(1144..>2187)
                     /gene="cfbE"
                     /locus_tag="PKK85_06090"
     CDS             complement(1144..>2187)
                     /gene="cfbE"
                     /locus_tag="PKK85_06090"
                     /inference="COORDINATES: protein motif:HMM:NF033197.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HNS25623.1"
                     /translation="GVKGKSSTVAILKEIYRHDNPLILSSLGVELVEGEKTTLLLKDI
                     SITPASIITAWKMALKNEFQPGICIFETSLGGTGLAEVGVLTNLAEDYPIARNSKRAS
                     HAKKQIFQSKKVVCHLKSFNSSYSSIKDFKDKTNTFFLEDEANITTSGVEYGIKETSF
                     TLEIKNLKTIGGELVNTSTRMKTFAPGPYHLENVLAAVGAAFTMGASEDDVRTGLEGF
                     KGIRGRSSIQESGKVRIVQEVNPGINLTAIKKSVAMMASYGDLVLVLGGQYGVTCEEI
                     DEKSLAVFLEGLTEDITLILTDELGKSLDGKLKRARVYVNYLEDALDLATELGVANVL
                     LIYRSHFASVEKR"
BASE COUNT          562 a          547 c          387 g          691 t
ORIGIN      
        1 ggtccctttc atcaaatccg atactgccat gaggttagcg tttttaagtc gcgagtacac
       61 ccgcacgtga ttatggccca tggcacccac accaattacg cccacattga cccttttcaa
      121 gagtacgcat cccccattat cctggcagct tctttaccta ctttttctgc tttttttaaa
      181 gggcccctaa cacttactct agatataacc tcaccttctt tggttaaaag tgccccatag
      241 agatccaaaa gatccccatc agcccgggca cacacaccca acggccactg gcaacccacc
      301 cccagatcct ggagcaccct tttttcagcg gtaacctctt gataagagat aaaatcacta
      361 agtcccttca gaacctcctt aacggaagaa tcctttcttg aaactatggc caaggctccc
      421 tgaccagctg ctggcgttat gtaatctatg gggaatgttt gttggatatg gtgtgccaga
      481 cccagtcttt tcaggccagc tgctgccatt aatgtggcat catattggcc gttttcaact
      541 tttcttaccc tggtatctat atttcccctg atgggtttaa taataacctt tttttggtgg
      601 aatttacaaa atgcctctct acgcaggctg ctggtaccca acgatgcatt ttcaggaagt
      661 tcatcccagg ggtaggcgga tatcagtgca tctcggggag tttctctagg gggtaccgct
      721 actatttcca agtcttcatt aagatccgtg ggcaagtcct tcaaactatg cactgcaaag
      781 tcaacttcct cttctaagag tgctttatcc aactcccggg taaatatccc cttggtatcc
      841 atgttatata gttgtgaatt ttttatttta tcaccagtgg ttttaatgac ctttatttca
      901 attttttcac tggtgagcct agataagtct gcgataacac cctttgtctg ggccagggct
      961 agaccacttc cccgtgtacc aaccttcaaa gaatcatctc cgagatcacc aaatagcaag
     1021 aaatcataag ctgtttaagt tttctaaagg ataacaagat aaattacgtt tattttttct
     1081 ttagtagatg atacttatgc atctttaata aatgttgccc aaccatggcc ccattctccc
     1141 aacctacctt ttctcaacac tggcaaagtg tgatcggtag atgagaagta cattagccac
     1201 acctaactca gtggccaagt ccagtgcatc ctctaaataa tttacataga ctctagccct
     1261 tttaagttta ccatcaagac ttttacccaa ttcatcagtg agaattagag taatatcttc
     1321 ggttaatccc tccaaaaata ctgcaagaga tttttcatca atttcttcac aggtaactcc
     1381 atattgcccc cccaacacca gaaccaagtc tccatagctg gccatcattg ccaccgattt
     1441 ctttatggca gtcagattga ttcctgggtt tacttcctgc actatcctta cctttcctga
     1501 ttcctgaatt gaacttctac ctcttattcc tttaaaacct tccaatccag tccgaacatc
     1561 atcttcagat gctcccatgg taaaagccgc tccaactgct gctaatacat tttccaggtg
     1621 ataaggtcca ggggcgaatg ttttcatccg tgtggaagtg ttcaccagtt ctcctccaat
     1681 ggttttaaga ttttttattt ccagggtgaa tgaagtctcc ttaatgccat attcaactcc
     1741 agaagtggtg atattggcct catcttccaa gaaaaatgtg ttagtcttat ctttaaagtc
     1801 tttaatagat gagtaactgg aattaaagga tttcaggtga catactacct ttttactctg
     1861 gaaaatttgt tttttagcat gactggccct tttagaattc ctggctatgg ggtaatcctc
     1921 agctaagttg gtgagcactc ccacctcggc cagtccagtg ccacccagtg atgtttcaaa
     1981 aatgcatatt cctggttgga actcgttttt taaggccatt ttccaggcgg tgatgatgct
     2041 ggccggagtt atgctgatgt ctttcagaag cagagttgtt ttttcccctt caaccaactc
     2101 tacacccaga ctactcaata tcaatggatt atcatggcga tatatttctt ttaatatagc
     2161 aactgtactg cttttgcctt taacacc
//
LOCUS       DAOQVW010000262         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_62796_length_2187_cov_2.891182, whole genome shotgun sequence.
ACCESSION   DAOQVW010000262 DAOQVW010000000
VERSION     DAOQVW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62796_length_2187_cov_2.891182"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..302)
                     /locus_tag="PKK85_06095"
     CDS             complement(33..302)
                     /locus_tag="PKK85_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS25624.1"
                     /translation="MTRREVKMYYDQALSFLGQGEAQKSVEFLDRALEIDDKYFPAWN
                     NKGIALLELGKFQDALKCFEQVIQLNSLDKMAWYNKGYTLLILEK"
     gene            457..1158
                     /locus_tag="PKK85_06100"
     CDS             457..1158
                     /locus_tag="PKK85_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS25625.1"
                     /translation="MRMVIVAGTPGSGKTAVLIHALRSLKEQGFKPAVVKVDCLYTDD
                     GKKFDKVGVPNLVGLSMDMCPDHYAIYNLEDMIKWAQENEADFLVIETAGLCHRCAPF
                     TRNSLGVCVIDATSGPNTPLKVGPFLSTSDIAVITKGDMISQAEREIFRERVLEVNPQ
                     CKIIDANGLSGQGCTELAEEMMKSQDVSLDDEKLRHSAPLAVCTLCVGETKVNKKYHR
                     GILRRIDGFQSYEGE"
     gene            1164..1847
                     /locus_tag="PKK85_06105"
     CDS             1164..1847
                     /locus_tag="PKK85_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNS25626.1"
                     /translation="MARKININQRDKLVQLLPGYNCGICGYARCDEFAASLLRESSKL
                     EKCRFMFQELFDENREKAETILKEEKIITPEEKIVGVLDGYEADFILKPLPGEHSCRE
                     VLYPFTREELKKGDIIRYRPLGCPIIHFGEILEENHGLITVHLVGPCHRVNPQVDFPF
                     KDVGVCMVGGFHGIIEGKLPGVGETVRFLPYHCMMQKVHSGVVVQLEGRKAVIEGIDL
                     KVWAPPIKG"
     gene            1874..>2187
                     /locus_tag="PKK85_06110"
     CDS             1874..>2187
                     /locus_tag="PKK85_06110"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNS25627.1"
                     /translation="MKKAIPTSPKSYKTIPIHTNYIHPNQPYDIIIEALEYNVEDNDF
                     LVISETPIAISQGRIVDESKFKPSWRAYLLADWWSKYLWGYILGPLLGMKRRTVKNLK
                     KLP"
BASE COUNT          675 a          456 c          459 g          597 t
ORIGIN      
        1 caaattaatc aataaaattt tcccctcatc cattactttt ctaaaatcaa aagcgtatat
       61 cctttattgt accaagccat cttatccagt gaatttagtt ggatcacctg ttcaaaacat
      121 ttaagggcat cttgaaattt tccaagctct aataaggcaa ttcccttatt attccaagct
      181 ggaaagtatt tatcatcaat ttccagggct ctatccaaaa attctacaga tttttgtgct
      241 tctccctgac ctagaaatga caaggcttgg tcatagtaca tcttcacttc tcttcgagtc
      301 atggtctcca cctcactaaa aatattattt actcccctaa aataatatgt ttaaataagt
      361 aaaatcttta agaataaaaa gaatagatct ttcttgttct attttcaatc gtattaagaa
      421 atagaaataa atttaaatca actatcaagt ggtaatatgc gaatggtaat cgtggctggt
      481 acccccggct ccggaaaaac agctgtactc atacatgccc tgcgcagcct caaagaacag
      541 ggttttaaac cagcggtggt aaaggtggac tgcttgtaca ccgatgatgg aaaaaaattc
      601 gacaaggtag gcgtacccaa cctagtgggc ctgtccatgg acatgtgccc tgaccactac
      661 gccatctaca acttggaaga catgatcaaa tgggctcaag aaaacgaagc cgattttctg
      721 gtaatagaaa ctgccggttt atgtcatcgt tgtgcccctt tcaccaggaa ttccctgggg
      781 gtatgcgtaa tcgatgccac cagtggtcct aacaccccac ttaaggtggg ccccttctta
      841 tccacctcag atattgcagt gataaccaag ggtgacatga tatcccaagc ggaaagggaa
      901 atattccggg aaagggttct tgaagtcaat ccccagtgta aaataattga cgccaatggt
      961 ttgagtggtc aaggttgcac tgaacttgcg gaagaaatga tgaaatcaca ggatgtttca
     1021 ctggatgatg aaaaactccg ccattccgct ccactagcgg tgtgcaccct gtgtgtggga
     1081 gaaaccaagg ttaacaaaaa gtaccatcgg ggtatcctgc gccgtataga tggattccag
     1141 agctacgagg gagaataggt ctcatggcta ggaagataaa tattaaccag agagataagc
     1201 tggttcaact attaccagga tacaactgtg gaatctgcgg ttacgcccgt tgtgacgaat
     1261 ttgcagcttc ccttttaaga gaaagttcta aattggaaaa gtgccggttc atgtttcagg
     1321 aactttttga tgagaaccgt gagaaagcag aaacaatact aaaagaagag aaaattataa
     1381 ctcccgaaga aaagatcgtg ggagttctgg acggctatga agcggatttc attttgaaac
     1441 ctcttcctgg agaacattcc tgccgagagg tgctctatcc cttcacccga gaagaactta
     1501 aaaagggtga cataattcgt taccgtccac taggctgccc cattatccat tttggtgaga
     1561 tactggaaga aaaccatgga ttaataaccg tgcacttggt ggggccctgt cacagggtca
     1621 atccccaagt tgattttcca ttcaaagatg taggagtctg tatggtgggt gggtttcatg
     1681 gaatcattga aggaaaactt ccaggggtag gggaaactgt ccgctttctt ccataccatt
     1741 gcatgatgca gaaggtgcac tccggagtag ttgtacaatt agaaggccgt aaagcagtta
     1801 ttgaggggat tgatctcaaa gtatgggcac ctcccatcaa gggttaattt ttaaaacttc
     1861 ttctggattt agcatgaaaa aagctattcc cacctcccca aaatcgtata agaccatccc
     1921 tattcacacc aactatatcc atcccaatca accttatgat ataatcattg aagcattgga
     1981 atataatgtg gaagacaatg actttctggt tatatcagaa actcccatag ccatatctca
     2041 gggaaggatc gttgatgaat caaagttcaa accatcttgg agagcttatt tgctagcaga
     2101 ttggtggtct aaatatctct ggggctacat cttgggtcct ttattgggga tgaaaagaag
     2161 gacagttaaa aatcttaaaa aactccc
//
LOCUS       DAOQVW010000263         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_63267_length_2177_cov_3.031103, whole genome shotgun sequence.
ACCESSION   DAOQVW010000263 DAOQVW010000000
VERSION     DAOQVW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63267_length_2177_cov_3.031103"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1711
                     /locus_tag="PKK85_06115"
     CDS             203..1711
                     /locus_tag="PKK85_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS25628.1"
                     /translation="MYVNMKKEDLGDIETTQDILIPKDPLERVIGHDDIIDFVKIAAK
                     QRRNLLLVGPPGIGKSLIAQAISFHLSEPQEEITVVHNPERPERPFVEIKNKKEMENH
                     KKDLERAEGDIVNPQDVPVMVAERLGFRCANCHSYNNAYQSICPQCGADKYSHINARR
                     KHLGDLLGMFEMSSNPMNVPQERVTTTRMLNGREEVVIYERMDGDKIKILDQSALEKR
                     RALVEEKPKNIILPLNRKVFIQATGATETELLGDVRHDPYGGHPDLGTQPYERVVPGA
                     VHEAHEGVLFIDELVHIAPLQRYILSAMQDKVFPIVGRNPQSAGSSVKVEDVPCDFIF
                     VAACNIRDVRYILPPLRSRIQGEGYEILMHTTMPDNTDNQAKMAQFVAQEIEIDGKIP
                     HATIKSVEILLDEARKRARVIDDQRDSFTLRLRDLGGVVRMAGDMAVMDGSKYIEPKH
                     MNFAVKKAISVEDQIINHYQSFEKAVLKDSSSQQLSSSNNKHSNENVDRSYL"
     gene            1790..>2177
                     /locus_tag="PKK85_06120"
     CDS             1790..>2177
                     /locus_tag="PKK85_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HNS25629.1"
                     /translation="MDTTGPSDIHIYRDNNILDAHDFPELIEDYLMELEIRNYSPNTI
                     RTYRSIINCFHRFLQGEVPENHEIFILKSFKRYIRYLKRDKKVSQNYIYLVTVVIKKF
                     FEFGEIGGLEDVKTPKRSKSLPKSLNE"
BASE COUNT          674 a          453 c          496 g          554 t
ORIGIN      
        1 attaataatc tgcaaaatgg ggcttatgct gtggtatttg atggtgtggt cagttagaga
       61 ctcattgatg tggctaaaga caagggcctc aagtacatag tagccgtacg tatgagtgaa
      121 gtggtaaaaa aacccaacca cattaaggtg ataaccagat aaacaacttt gactggggaa
      181 ctgacctcgg aggatgatct aggtgtacgt caacatgaaa aaggaggatt taggagatat
      241 tgaaacaacc caggatattc taattcctaa agatccctta gaaagggtca ttggccatga
      301 tgacatcata gattttgtta aaatcgcagc caagcaacgt cgaaacctgc tgctggtggg
      361 ccctccaggg ataggtaaat cactgatagc tcaggccata tcattccatc tttctgaacc
      421 ccaggaagaa ataacggtgg tccataatcc ggaaaggcct gaacggccct tcgtggagat
      481 caaaaataaa aaagagatgg aaaatcataa aaaagatctg gaacgggctg agggagatat
      541 tgtaaacccc caggacgttc ccgtgatggt ggccgagaga ttgggtttcc gctgtgccaa
      601 ttgtcatagt tacaacaatg cctatcaaag tatctgtccc caatgtgggg ctgataaata
      661 ttctcacatc aacgcacgac gaaaacatct gggcgacctt ttgggcatgt ttgagatgag
      721 ttccaaccca atgaacgttc cccaggaaag agtaaccacc acccgtatgc tcaatggccg
      781 agaagaagtg gttatctatg agaggatgga tggggataag attaagatcc tggatcagag
      841 tgcactggag aagagaaggg ccctggttga agaaaagccc aaaaacatca tactccccct
      901 taaccggaag gtattcatac aggccaccgg ggccacagaa actgaactgt taggtgatgt
      961 ccggcatgat ccctatggtg ggcatcctga tcttgggacc caaccatacg aaagagttgt
     1021 tcctggtgca gttcacgagg cacacgaggg tgttcttttc attgacgaac ttgtgcacat
     1081 tgcccctctc cagcgataca ttctaagtgc catgcaagac aaggtttttc ccattgtagg
     1141 ccgtaatcct cagagcgcag gaagttcagt taaggtggaa gacgtgccct gcgacttcat
     1201 atttgttgcc gcttgcaata taagggatgt tagatatata ttaccccctt tgcgttctcg
     1261 tatacaggga gagggttatg aaattctaat gcacactacc atgcctgata acactgataa
     1321 ccaggctaaa atggcccaat ttgtggctca agagattgaa attgatggaa aaattcccca
     1381 tgctactata aaatccgtag aaatccttct ggatgaagct cgcaaacgag cccgggtaat
     1441 tgatgatcaa cgagattcat ttacacttcg acttcgtgat ctgggaggtg tggtgcgtat
     1501 ggctggagat atggcggtta tggatggtag taaatatatt gagcctaaac acatgaattt
     1561 tgctgtgaaa aaggccatat ctgttgaaga ccagataatt aatcattatc agtcctttga
     1621 aaaggcagtt ctaaaggatt ctagttccca gcaattaagt tcttcaaata acaagcactc
     1681 caacgaaaac gtggatcgta gttatctata attagttaaa ctgcccataa tatccatcaa
     1741 catgaactct taccccaact caacttggaa agttaaacca ggaggttcag tggatacaac
     1801 gggcccatct gatattcata tttacagaga taacaacatc ctggatgccc atgacttccc
     1861 cgagctaatt gaagattacc tgatggaact ggagattcgc aactattccc ccaacaccat
     1921 ccgaacctac cgttccataa tcaactgttt tcaccggttt ctgcaaggtg aggttccgga
     1981 aaatcatgaa atttttattt taaaatcttt caaacgttat atccgctacc ttaaacggga
     2041 taaaaaggtt tctcagaact acatatactt ggtgacagtg gttataaaga aattttttga
     2101 atttggtgag ataggggggc tggaagatgt caagacccct aaaagatcca aatctctacc
     2161 gaagtccctt aacgaag
//
LOCUS       DAOQVW010000264         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_63686_length_2169_cov_2.782403, whole genome shotgun sequence.
ACCESSION   DAOQVW010000264 DAOQVW010000000
VERSION     DAOQVW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63686_length_2169_cov_2.782403"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PKK85_06125"
     CDS             <1..821
                     /locus_tag="PKK85_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS25630.1"
                     /translation="LGPNGAGKTTTVRMLTGIIKPQDGSIRIMGHDIQREPLEAKEHL
                     GVVPETSNAYVDLSAWQNLMLMAGLYGVPSDVSHERAQDLLTEFGLYPRRDDKVKGFS
                     KGMKQRLILAMALINDPELLFLDEPTSGLDVQSSILIRQMLMQLRGKGKTIFLTTHNL
                     EEANQLCDRIAIINHGKIAVIDSPHNLKKMTRKLNTIEVTFSTGVDLAELSRAPGVLE
                     VKDTGEKIVMNTENVNQALSFLTDYAHSRGIQIQSLNTLAPSLEDVFMEFVGGP"
     gene            808..1575
                     /locus_tag="PKK85_06130"
     CDS             808..1575
                     /locus_tag="PKK85_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS25631.1"
                     /translation="MEDLKVHSLYLDQFKRSLSIMKKDILIYYLKGPVIIFGVLIPLF
                     LFLAFLTGSKNLSTDFLISGLIGMTMLFTATSVSPVIIPWESQMKTLERLMACPISIH
                     TLIMGDMLASVLFGFFISLVPVLIGLALDVIPIHTLVLVMGIGLASLCFSSLGLLLAT
                     PPTNAPSNVMMISSLIKFPLVFISGIFIPLESMPFWGKIIASLSPLTYFTDITRYSLQ
                     NVSYYPIWLDFLAIILFTLMFFVLAVKIHKKTLAMRI"
     gene            1594..2046
                     /locus_tag="PKK85_06135"
     CDS             1594..2046
                     /locus_tag="PKK85_06135"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNS25632.1"
                     /translation="MSKEKEKVLFICRNNSGRSQLAEGLLRHLYGDHYQVWSAGLDPR
                     PINPLTLEVLEELGVDTVTLESEGLEKFMDQEFDWVVLLCAGEGDECPVSLSAAVFIQ
                     KVFPDPRDIPVEGDQTLEEKLQQFRVIRDQIQNWVKEQFKPSPPPKKK"
BASE COUNT          537 a          602 c          520 g          510 t
ORIGIN      
        1 tcctgggccc caacggggcg ggtaagacca ccaccgttag gatgttaacg ggaatcatca
       61 aaccccagga tggatccatc cgtatcatgg gacacgacat ccaaagggag cccctggaag
      121 ccaaggaaca tctgggggtg gtgccggaaa cctccaatgc ctacgtggat ctttcggcct
      181 ggcagaacct gatgctaatg gccgggctct acggagtccc ctctgatgtc tcccatgaac
      241 gggcccagga tcttttaact gaattcggcc tctacccccg tagggatgat aaggttaagg
      301 gcttctccaa gggtatgaag cagaggctga tcctggccat ggccctaata aacgacccgg
      361 agctcctgtt tctagacgaa cccaccagtg gactggatgt ccagagcagc atcctcatta
      421 ggcagatgct catgcaactc cgtgggaagg gtaaaaccat cttcctcacc acccataacc
      481 tggaggaagc caaccagctc tgcgacagga tagccatcat caaccatgga aaaattgccg
      541 taatagacag tccccataac ctcaagaaaa tgaccaggaa gttgaacacc atcgaggtaa
      601 ccttcagcac aggggtggac ttggctgagt tatcccgggc accaggagtc ctggaggtga
      661 aggatacagg ggaaaaaatc gtcatgaaca ctgaaaacgt taaccaagcc ctgagttttc
      721 tcactgatta tgcccattcc aggggtatcc agatccagtc cctcaacacc ctggcaccca
      781 gcctggaaga tgtgttcatg gaatttgttg gaggacctta aggtgcacag cctctacctg
      841 gaccagttca aacgttccct gtccatcatg aaaaaggaca tcctgatcta ctacctgaag
      901 gggccagtaa tcatatttgg ggtactgata ccattgttcc tgtttctggc cttcttaact
      961 ggtagtaaga atttgtccac tgatttcctc atctcgggcc tcatcggaat gaccatgctc
     1021 ttcaccgcca cctcggtgtc cccggttata attccctggg agtcgcagat gaagaccctg
     1081 gagcggctga tggcctgtcc catatcaatt cacaccctaa tcatgggtga catgttggcc
     1141 tcggttctat tcgggttttt catctccctg gtaccagtac ttattggact ggcattggat
     1201 gtcatcccca tccataccct ggttctcgtt atggggatcg gcctggcttc cctgtgcttc
     1261 tcatcactgg gcctcttact ggccactcca cccaccaacg ctccctccaa cgtcatgatg
     1321 atcagctccc tcatcaagtt ccccctggta ttcatcagcg gcatcttcat ccccctggag
     1381 agcatgccct tttggggtaa gatcatagct tccctatccc ccctaaccta cttcacggat
     1441 attacccgtt actccctgca aaacgttagt tactatccca tatggctgga cttcttagcc
     1501 atcatcctat tcaccctgat gttctttgtc ctggctgtga agatccacaa gaaaacctta
     1561 gccatgcgta tctagatcca aaggtgataa taaatgagta aagaaaaaga aaaggtgctc
     1621 ttcatctgcc gtaataactc cggccggtcc caactggcgg agggccttct tcggcacctc
     1681 tacggtgacc actaccaagt ctggagtgct ggcctggatc cccggcccat caaccccctg
     1741 accctagaag tcctggagga gcttggggtt gatacagtta ccctggagtc agagggcctt
     1801 gaaaaattca tggaccagga atttgactgg gtggtcttat tatgtgctgg ggaaggggat
     1861 gagtgccccg tatccttaag tgctgctgtg tttatacaga aggtattccc ggatccccgg
     1921 gacatccctg tagaggggga ccagaccctg gaggaaaagt tgcaacagtt cagagtcatc
     1981 cgggaccaga tccagaactg ggtcaaggag cagtttaagc catcaccccc acctaaaaag
     2041 aagtgatacc tatgaaagta gccatagtaa gtgacggccc ctatggagaa cgggcctacg
     2101 ccaccattaa agaagaattt gaaaccgaat ttatagtact ggaacccccc atggatgcct
     2161 tcgctgatg
//
LOCUS       DAOQVW010000265         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_64834_length_2144_cov_3.070847, whole genome shotgun sequence.
ACCESSION   DAOQVW010000265 DAOQVW010000000
VERSION     DAOQVW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64834_length_2144_cov_3.070847"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PKK85_06140"
     CDS             <1..853
                     /locus_tag="PKK85_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS25633.1"
                     /translation="HIDVDPAEIGKNIPVDVPIVGDAKIILSHLIRVISQVESTQKKE
                     WTEHVKQFRSSCLPRLSFDDTPLKPQQVIKEISEAVTDDTILTTDVGQNQMWMAHYFT
                     SRNPRKFISSGGLGTMGFGFPAAMGAKVALPEEDVIAVCGDGGFLMVCQDLATVKEYD
                     IPVVVCVLDNRYLGMVAQWQKLFYQERISHTELGEVPDFVKLAEAFGVNAYRVKKPGE
                     MKETLKEALNSGEPTLIDVIIDPNEILPMVPPGCGLTEMVGEYKVEREVPGEIPYRSS
                     AAERGGD"
     gene            855..1355
                     /gene="ilvN"
                     /locus_tag="PKK85_06145"
     CDS             855..1355
                     /gene="ilvN"
                     /locus_tag="PKK85_06145"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNS25634.1"
                     /translation="MTNERSHIISAVVLHRPGVLQRVAGLFTRRGFNIDAITVGPSEK
                     DGVARMTIISRGDEKVLEQITKQLNKLIEVIKVRDLDPPSTVLRELCLIKTHASHEKA
                     RSEIIQYANIFRGRIIDVSPVNLTIEITGTPEKIDALIDLLQNFGIQEIARTGPTAIT
                     RGAKTI"
     gene            1520..>2144
                     /gene="ilvC"
                     /locus_tag="PKK85_06150"
     CDS             1520..>2144
                     /gene="ilvC"
                     /locus_tag="PKK85_06150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNS25635.1"
                     /translation="MKIYYEKDVNMDVLKDKTVAVIGYGSQGRAQAMNMAESGLNVVV
                     GLRPDGGSWKVAEEDGLEVMTVDEASQESDIIHLLIPDEIQAQVFEGSIEEGLEEGNT
                     LSFSHGYNIHFQYIKPPENVNVTMIAPKGPGSTVRQQYVDGFGVPGLVAVEQDYTGDA
                     MAIALAMGQGCGLTRAGVLETSFKEETETDLFGEQAVLCGGVTELIKA"
BASE COUNT          643 a          449 c          548 g          504 t
ORIGIN      
        1 ccacatcgat gtagatcccg cggaaatcgg taaaaacatc cccgttgatg tgcccattgt
       61 aggagatgcc aagatcatcc tatcccatct gataagggta atatctcaag ttgaaagcac
      121 ccagaaaaag gagtggaccg aacatgttaa acaattccgc agcagctgcc tcccccgatt
      181 atcatttgat gatactcctt taaagccaca acaggttatt aaagagattt ctgaagcagt
      241 gacagatgat accatactaa ccaccgatgt ggggcaaaac cagatgtgga tggcccatta
      301 tttcacttcc cgaaatccta ggaagtttat atcatccggg ggactgggaa ccatgggatt
      361 cggtttccca gctgccatgg gggctaaggt agcccttcca gaagaggatg tgattgcagt
      421 atgtggtgac ggcggatttt taatggtctg tcaggattta gctaccgtca aagagtacga
      481 tattccagtg gtggtttgcg ttcttgataa ccgctacttg gggatggtag cccagtggca
      541 gaagctcttc taccaggaac gaatatccca caccgaactg ggcgaagttc ccgactttgt
      601 aaagctggcc gaagcctttg gagtcaatgc ttaccgggtg aaaaaaccag gagagatgaa
      661 ggaaaccctc aaggaagccc ttaactcagg agaacccaca ctaatcgatg taataattga
      721 tcctaatgag atcttgccta tggtgccgcc gggttgtgga ttaactgaga tggtgggaga
      781 atataaagta gagcgggaag ttccagggga aataccctac cggtcttcgg cagccgagag
      841 gggaggtgat tagaatgact aacgaacgaa gccacataat aagtgccgtg gtcctgcacc
      901 gcccaggggt gctgcagaga gtggctggac tcttcaccag acggggattt aacatcgacg
      961 ccataaccgt cgggccatcg gaaaaggatg gtgtggcccg catgaccatc atttcccggg
     1021 gagacgagaa ggtcctagaa caaataacca aacagttgaa caagctcatc gaagtcataa
     1081 aggtcaggga cttggacccc ccatcaacgg ttttaagaga actttgcttg ataaaaaccc
     1141 acgcatccca cgaaaaggct cgttcagaga taattcagta cgccaacata ttccggggga
     1201 ggatcatcga cgttagtccg gttaacctca ccatcgaaat caccggcacc cccgagaaga
     1261 ttgatgcgct cattgacctc cttcagaatt ttggaatcca agaaatagcc agaaccggcc
     1321 caactgccat tactagagga gcaaaaacca tctgagggat aagatatact gttggatgaa
     1381 ttttatttgg gatcttggaa ttgaaatgga gttgcgtgaa cgggaaaaat attatataaa
     1441 tgaacttccc ttaactacta aacagaaaaa acttggtttt aaactaattt aagacttcta
     1501 atatcgaaaa ggcggttata tgaaaattta ttatgaaaaa gacgtgaata tggatgtttt
     1561 gaaggataaa accgttgcag tgataggtta cggtagccag ggaagggctc aagccatgaa
     1621 catggctgaa agtggtctta atgtagtggt tgggctgcgc ccggatggtg gatcctggaa
     1681 agtagctgaa gaagatggtc tagaagttat gactgtggat gaggcctccc aggaatctga
     1741 catcatccat ctgctcatac ctgatgaaat acaagcccaa gtatttgaag gatccataga
     1801 agagggattg gaagaaggaa acaccctgtc attttcccat ggatacaaca tccacttcca
     1861 gtacattaaa ccacctgaaa acgttaatgt taccatgata gcgcctaaag ggccaggttc
     1921 cacagttcga cagcaatatg ttgatggatt cggagttcca ggattggtag ctgtggaaca
     1981 ggattatact ggagatgcta tggctattgc tctggccatg gggcaaggtt gcggtttaac
     2041 ccgtgctggt gtattagaaa cgagtttcaa agaagaaaca gaaactgatc ttttcggtga
     2101 gcaggcagtt ctgtgtggag gggtcactga actgataaaa gccg
//
LOCUS       DAOQVW010000266         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_64891_length_2143_cov_2.943008, whole genome shotgun sequence.
ACCESSION   DAOQVW010000266 DAOQVW010000000
VERSION     DAOQVW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64891_length_2143_cov_2.943008"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..568)
                     /locus_tag="PKK85_06155"
     CDS             complement(41..568)
                     /locus_tag="PKK85_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNS25636.1"
                     /translation="MSNYEPLILKDAIDEIIDNVKKVSDALDPENIQTMNNILKTSQN
                     VFVMGLGRSGLMARAFAMRLMHLEISVYVVGETITPAIMGDDCLLAISGSGETLSIIS
                     AAKIAQKRKAKIIAVTSYVDSTLGKMADLVVHVGGRTKIDSEKNYLARQINGKHQSLS
                     PLGTLFEVTTLVFCH"
     gene            653..1513
                     /locus_tag="PKK85_06160"
     CDS             653..1513
                     /locus_tag="PKK85_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNS25637.1"
                     /translation="MDIQPKLNLVINGHNFSYKLFETLHCISKTFSQRKAAKRLGISH
                     AVLNRRIKDAEAKLGFLLVESSGSGSVLTSHGLEVIAQYQQLMKRLSEHKQPVICGGH
                     ISTGLMDSLCHNYGLDSVIYSTDDLSALKLAEMGLVDILTLDDPVHVFMRDLDFTPLA
                     YDYLVLVSPPGTEVNSLKDLEGQNFVEVPHSAQRLAWNTLDHLKIDYNITEIYNSPYT
                     AFKKVQDSQDLYSFLNRSLTTGWEMFADDTRHLLTSVLCSTQSRLESFIDYVSGRGQR
                     VVEDRGFERI"
     gene            1612..>2143
                     /locus_tag="PKK85_06165"
     CDS             1612..>2143
                     /locus_tag="PKK85_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNS25638.1"
                     /translation="MNYNRDLLAIGHTAFDYIIKVNEFPHANSSTAITRMKTFYGGAA
                     ANVAMVASKLGLKTYLVSAVGEDYPGSEYEQQMKHMDIDTSHKILIKEDKTPTAFVFT
                     NKDHDQISYFYWGAATHFKNAKPPLNAIRDVKAVHLATGDPNFNWRCGEVARQHDKLI
                     SFDPGQDLHMYSIKELR"
BASE COUNT          634 a          445 c          445 g          619 t
ORIGIN      
        1 aaatgcacct gcccggtcaa accatttgtt ttccgtaagg tcaatggcag aagaccaaag
       61 tggtaacctc aaataatgtt cccagcgggg ataaggactg atgcttacca ttaatttgcc
      121 gtgctaaata gttcttttca gaatcaatct tggtacgccc tcccacgtgc accaccagat
      181 cagccatctt tcccagtgta gaatccacgt aagatgttac ggcaataatc ttagcctttc
      241 ttttctgggc tattttagca gcactgatta tggaaagtgt ttcacctgag ccagatattg
      301 ccagtagaca atcatcaccc ataatggcag gtgttatggt ttcacccacc acgtagacac
      361 taatttcaag atgcatgagg cgcatggcaa atgctcgcgc catcaaaccg gaacggccca
      421 atcccataac aaatacgttc tgggaagttt tcagtatgtt gttcatggtt tgtatgtttt
      481 cagggtccag agcatccgaa acttttttta cattgtctat tatctcatct attgcatcct
      541 ttagaatcaa gggttcataa ttagacatca tcttcatctc ttatatctga agatggatat
      601 aaataatttc atatgagtaa ggggatgttc cgaaaacttc tccggtgtta ccatggatat
      661 acaacccaag ctgaatttag taatcaatgg ccataatttt agttacaaac tattcgaaac
      721 ccttcattgt atttcaaaaa cattttccca gagaaaggca gcgaaacgtc tgggaatatc
      781 tcatgcagtt ttaaataggc gcataaaaga tgctgaagcc aagttgggct ttttactggt
      841 ggaaagctcc ggttcgggat cggtgctgac cagtcatggt ttggaagtaa ttgcacagta
      901 ccagcagtta atgaaacggt tgtccgagca taaacaacca gttatatgcg gaggacacat
      961 ctcaacaggc cttatggata gtttatgtca taattatggt ttagactctg taatttatag
     1021 cactgatgat ctaagtgccc tgaagttagc agagatgggc ttggtagata ttttaaccct
     1081 tgatgaccca gtgcatgttt ttatgcggga tttagacttt acaccccttg cttacgacta
     1141 tctagtcctg gtatcccctc ctgggacgga agttaatagt ttaaaggatc tggagggtca
     1201 aaatttcgtg gaagtacccc attctgcaca gagattggct tggaacaccc ttgatcattt
     1261 aaagattgat tacaacatta cagaaattta taactcaccc tacacagctt ttaagaaagt
     1321 acaggatagc caagatcttt acagcttcct gaatcgcagt ttaaccaccg ggtgggaaat
     1381 gttcgcagat gacacgcgtc atttattaac atcagtgctt tgcagtaccc aaagtaggct
     1441 ggaatcgttt atagattatg tgtctggcag gggccagcgg gtagttgaag atcggggctt
     1501 tgaaagaatt tagttatttt catggcaata ctattctcta tgtaaattca ttagccagca
     1561 cccacactaa atataaatat cctaaaacta aacatagccg tggtgattta tctgaattat
     1621 aatagggatt tgctggctat tggccataca gcctttgatt acatcataaa agttaatgaa
     1681 tttcctcatg ctaactcatc cactgctata acccgtatga aaacatttta tggtggagca
     1741 gcggctaatg tggccatggt ggcctctaaa ctcggactga agacctatct ggtttcagcg
     1801 gtaggtgaag attaccctgg ctccgaatac gaacagcaga tgaaacatat ggatatcgat
     1861 accagccaca agattctgat taaggaagat aaaaccccca ccgcatttgt atttaccaac
     1921 aaagaccatg atcagatcag ttacttctat tggggtgcag ctacccattt taaaaacgcg
     1981 aaaccaccac tcaatgcaat tagggatgtg aaagctgttc atctggctac tggcgatcca
     2041 aattttaact ggagatgtgg agaagttgct cgccagcatg acaagttaat ttcatttgat
     2101 cccggacagg atctgcacat gtacagcata aaggaattaa ggg
//
LOCUS       DAOQVW010000267         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_65086_length_2139_cov_2.284069, whole genome shotgun sequence.
ACCESSION   DAOQVW010000267 DAOQVW010000000
VERSION     DAOQVW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65086_length_2139_cov_2.284069"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PKK85_06170"
     CDS             complement(<1..998)
                     /locus_tag="PKK85_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS25639.1"
                     /translation="MRLLKDFIFPFTAIVGQENIKKSLILNAINPSIGGVLIKGEKGT
                     GKTTAVRALADLLPPIKVVKGCPFNCDPEDTSSICQSCKSNDFEIEEKKMRVVELPLG
                     ATEDRVVGSINIEKALKEGVKALEPGLLAEANRNILYVDEINLLDDNLVDVLLDAAAY
                     GINIVEREGVSFTHPSNFILVGTMNPAEGELRPQLSDRIGLHIVVSSIMELEDRVKIM
                     ERREEYEKYPEKFQEKFQEKQEAVRQSIVKARKILKNMEIPRYLLEVIAQLCMDMGVD
                     GHRADIAILKTAKTIAAYNDQTTVTEDHVEEAVLLVLGERFHATSFDKDKVKKQIE"
     gene            1180..1335
                     /locus_tag="PKK85_06175"
     CDS             1180..1335
                     /locus_tag="PKK85_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25640.1"
                     /translation="MCTVGGPMADIKMKKLKTKKYRCLDCGNEFKGIGKRVVCPSCQS
                     DNVEESS"
     gene            1350..1775
                     /locus_tag="PKK85_06180"
     CDS             1350..1775
                     /locus_tag="PKK85_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25641.1"
                     /translation="MFLKGSSGELGIVKASKDLQIFIGTPDGEEVVQFLQANDLIAVS
                     VFSVGEMAEKGVRCMVHLLKDHASPIVVLPENHPTSKRLSMVVSCGDLIRLDCGIQPG
                     THPEQDILCACEDLSGVEIRAVNGGVEIKGSADFIVEKL"
     gene            1930..>2139
                     /locus_tag="PKK85_06185"
     CDS             1930..>2139
                     /locus_tag="PKK85_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25642.1"
                     /translation="MSIYDFYQILGQLVFIVGLFLLVLLSTTLILGRMQIKENRLIFP
                     RLLLFTVDVFYGLFKKFSEQVGVDGK"
BASE COUNT          591 a          435 c          421 g          692 t
ORIGIN      
        1 tgttctatct gctttttaac tttatcttta tcaaatgatg ttgcgtggaa tctctcaccc
       61 aataccagga ggacagcttc ttccacgtgg tcctcagtta cggtggtttg gtcgttgtag
      121 gcggcgattg ttttggctgt ttttaaaatt gcgatgtccg cccggtggcc gtctacgccc
      181 atatccatac ataactgggc tataacttcc agcagataac gtggaatttc catgttcttc
      241 agtattttcc gggctttgac tatactctgc ctgactgctt cctgtttttc ctggaatttc
      301 tcctggaatt tctcaggata cttttcatat tcttcccttc tctccattat cttaacccgg
      361 tcttccagtt ccatgatgct gctgaccacg atgtgcagcc ctatcctatc tgaaagctgg
      421 gggcggagct ctccctctgc cgggttcatg gtccctacca gtatgaagtt ggatgggtgg
      481 gtgaaggaaa ctccttccct ttccactatg tttataccat aggctgcggc gtccagtagg
      541 acatccacca ggttatcgtc caggagattg atttcatcca catataatat gttacggtta
      601 gcttctgcca gtaaaccggg ttcgagggct tttacacctt cctttaaggc cttttctata
      661 tttatggaac caaccactct gtcttcagtg gcccccagag gtaattctac caccctcatt
      721 tttttctctt caatctcgaa atcattagat ttgcaagact ggcagattga actggtatct
      781 tccgggtcgc agttaaatgg acacccttta acaactttta tagggggaag taagtctgcc
      841 agtgccctaa cagccgtggt cttaccggta cccttctccc cttttattaa aaccccaccg
      901 atacttggat ttatggcgtt taatatcagg gattttttta tattttcttg tcctactatg
      961 gcggtaaatg ggaaaataaa atctttcaat agtctcaccg tttattgctg gttttattct
     1021 ttagcttcaa ccttttaaaa attccaagtt aatacttgtt aaattttttg ttgttcattg
     1081 gtattaaaaa tcttgtttta aataaaataa aaaattgttt aaaaatgtta cacaaaagat
     1141 aaatatccca gtttacatac ctcacattgg tgatatagaa tgtgtactgt tggcggtcca
     1201 atggccgaca ttaaaatgaa aaaactaaaa actaaaaagt accggtgcct ggattgtgga
     1261 aatgaattta aaggtattgg aaaaagagta gtatgtcctt catgtcagtc agacaatgta
     1321 gaagagtcaa gttaagtcct gatgaaattt tgtttttaaa gggaagctct ggagaactgg
     1381 gaatagtcaa agcctcaaaa gacctgcaga tattcatagg aacacctgat ggcgaagaag
     1441 tagtccaatt tctccaggcc aatgatttga tagctgtttc tgtattttct gtcggggaaa
     1501 tggcagaaaa aggtgtaaga tgtatggtcc atctcctgaa agatcatgcc tctcccatcg
     1561 ttgttctacc agaaaaccat cccacatcca agagactcag catggtcgta tcctgcggag
     1621 atcttatccg actggattgc ggcatacaac caggtactca tcctgaacag gatattctct
     1681 gcgcctgcga agacctctcc ggggttgaaa tacgggccgt gaacggtggt gtggagataa
     1741 agggatccgc tgattttata gttgaaaaat tgtaatttag aattaaataa taactaaata
     1801 attaagttaa taatccggaa cttcaaaatt tcattaagtt cgaatgaaaa acattaaatt
     1861 agaataaatt aaatttttct ctaaaggtta aaataagtaa gatatatctt taaaaaaaag
     1921 gtcaccccta tgtcgattta tgatttttac caaatactcg gacagttagt atttatcgta
     1981 ggcttatttc tcctagtttt gctatcaacc accctcattc tgggcagaat gcaaattaag
     2041 gagaatagat taatttttcc cagattactc cttttcactg tggatgtttt ctacggcctg
     2101 tttaagaaat tctcagaaca agtgggtgtc gatgggaag
//
LOCUS       DAOQVW010000268         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_65102_length_2138_cov_3.931349, whole genome shotgun sequence.
ACCESSION   DAOQVW010000268 DAOQVW010000000
VERSION     DAOQVW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65102_length_2138_cov_3.931349"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2138)
                     /locus_tag="PKK85_06190"
     CDS             complement(<1..>2138)
                     /locus_tag="PKK85_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNS25643.1"
                     /translation="LGREVTKKADQLLNFATADDVLCITTAGLAYRNNITTEDCLEGI
                     LSGSNGKISYGQGNLLTFQSVRTDPVDFCFIVKKGAVLTAAFFKNGSLNPDYLGTFSA
                     IDQNLWEKTITPKLGKNAFGYVSLAHAWKEGLSSDILRQAAYHGHVCLGTITGQAMVS
                     TLLKYYPPGEYGNQNELEATVYRAISVPGGSDDDAYIYSLDLTTGKRSWVGYSTAEDN
                     MVGFIRWSDTLKKGTLIIMRFNEEKVLQDYKKETSLNAYTGIVGELNFNTWLIKKLEN
                     DPDSLVEILYVFENITPEIHNNLTGGADSKTVVTDALGLDMTYILGLGLTNIADKKKA
                     TNYETGNLSLDTIKNIGINAANMAKELFETENGVKLEKDDPKLTVFTSAGYVRIKGQV
                     MDMTMDGIYQVLGSRLSRATLLPVHTARHNPLYFQFSYENPNGTVTSKSIYYDPETGA
                     LTAKNESACIIDQVILYDPPYDALMAWLWHNHVCGGSSPGYLITNYIYENFPIGEDES
                     YSLIGNSISCRDDIYSYLLGISPGLGTYYSQRMTSKTTGNDILVLSVYDSKTKTRRVA
                     IINWKNPSFEKGSNSYEEYIRLYWGDTSSPNLKSAPVISLTADTWVTEEEWAKIIAGG
                     EGSLNTLEYIKGLPVRSQQDLIKMQGGSQIDGPTIPGTESSGSGSSPGSSIISRGGSV
                     GDSGAAVSAVSVSAAVGEEAGDSGKAYEVT"
BASE COUNT          493 a          478 c          494 g          673 t
ORIGIN      
        1 gggttacttc gtaggctttt ccactgtcac cagcttcttc tcctacggca gcactaactg
       61 acacagcact aactgcggct ccagagtcac caactgatcc tccgcgggat atgatacttg
      121 atcctgggga agaaccggat ccactggatt cagttcccgg gatggttgga ccatcaatct
      181 gggaacctcc ctgcattttt ataaggtcct gctgactacg tacaggtagg cccttaatgt
      241 attcaagagt attgagtgat ccctcacctc cggctatgat tttagcccac tcttcttccg
      301 ttacccaagt atctgcagtt aaggaaatta ctggtgcaga cttcagattt ggtgatgaag
      361 tatcacccca gtacaacctt atgtattctt cataagagtt ggaacccttt tcaaatgaag
      421 ggtttttcca gtttatgatg gctactcttc tggttttggt tttgctgtcg tagacactga
      481 gaaccagaat atcgtttcct gtggttttac tggtcattct ctggctatag taagttccta
      541 atcctgggga tattcctagg agataggagt agatgtcatc tctgcagctg atggagttac
      601 caattaacga gtaactttca tcttctccaa ttgggaagtt ttcgtagatg tagttagtaa
      661 tcaaatatcc tggtgaacta cctccacaga cgtggttgtg ccataaccag gccataaggg
      721 catcatatgg tggatcatac agtatcactt ggtcgattat acaggcagat tcgttcttag
      781 cagttaatgc tccggtttct ggatcgtagt atatgctttt actggtcaca gttccattgg
      841 gattttcata actgaattgg aagtacaatg gattgtgtcg ggcagtgtgc actggtagta
      901 gagtggctct gcttagtctg gaaccaagaa cttggtatat tccatccatg gtcatatcca
      961 tgacctggcc cttgatccgt acgtaaccgg cacttgtgaa gaccgtgagc ttagggtcat
     1021 ccttctccag tttaactcca ttttcggttt caaacagttc cttggccatg ttggctgcat
     1081 tgataccgat gttcttgatt gtatcaagtg ataggtttcc ggtttcgtag ttagtggctt
     1141 ttttcttgtc agcgatgtta gtcaatccta agccaagtat gtaggtcata tccagcccta
     1201 aagcatcggt aactaccgtc ttggagtctg ctccaccggt tagattgttg tggatctctg
     1261 gggtgatgtt ttcgaatacg tatagtatct caactaagct gtcgggatca ttttccagtt
     1321 tctttattaa ccaggtgttg aaattcagtt ctccaacgat accggtatag gcgtttaagc
     1381 tggtttcctt tttgtagtcc tgaagcactt tttcttcgtt gaacctcatg attatgaggg
     1441 ttcccttctt aagggtgtca ctccagcgta tgaaacctac catgttatcc tcggcagtgg
     1501 aatatcccac ccaggaacgt ttcccagtag ttaaatcaag ggaatagatg taggcatcgt
     1561 catctgatcc tccggggaca ctgatggctc gataaacagt ggcttctaat tcgttttgat
     1621 ttccgtactc acctggtgga tagtatttca gaagcgtact aaccatggcc tgtccagtta
     1681 tggttcccag acacacgtgg ccgtggtagg cggcctgtct tagaatatca ctggagagtc
     1741 cctctttcca ggcatgagcc agacttacgt agccgaatgc gttcttacct aattttggtg
     1801 ttatagtttt ttcccataag ttctgatcga tggctgagaa ggtacctaaa tagtccgggt
     1861 ttagtgaacc gttcttgaag aatgcagctg taagtacagc gcctttctta acaatgaaac
     1921 agaaatccac gggatctgtt cggactgatt ggaaagtcag caggttaccc tgtccgtagc
     1981 tgattttgcc attggagcca ctcagaatac cttctaaaca atcttccgtg gtgatgttgt
     2041 ttctgtaagc cagccccgct gtggtgatgc acagtacatc atcggctgtg gcgaagttca
     2101 agagctggtc agctttcttg gtgacttctc ggcccagc
//
LOCUS       DAOQVW010000269         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_66100_length_2118_cov_2.862821, whole genome shotgun sequence.
ACCESSION   DAOQVW010000269 DAOQVW010000000
VERSION     DAOQVW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66100_length_2118_cov_2.862821"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="PKK85_06195"
     CDS             <1..1544
                     /locus_tag="PKK85_06195"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HNS25644.1"
                     /translation="DIKSCSLLKLKNLTRDVWDLRFFILLFLGFLALYIFSLFKLDNY
                     LYPKAELILLVVVTLLGTFCITYYSKRKGQLHRVALVVILSFGLVSVFLTPLLDVLDE
                     REHFTRAEMTSRGVIFPEYDYSTGTYQTIKSVEDMHNRHSYPHLTVFNTDFDSRKIDD
                     NPGQLKSAFVQNPFFGYLAPAVGIKVAKLLDLNAVWMLWLGRLFNLILYALVSAYAIK
                     KSPIFKMQLMAVACLPLAIYYAASTSIDAMINSFALLVIGYFFYMYKSADYSLDWRHL
                     LVFFTLCLVCGFCKVTYMALSLLVLVVPLSRFKLKRYYLAGGLGIVGLLLLTISWTSY
                     VLPLSYNSWRGNSYLLRGVNSTQQVNYMLSNPAQTGVMFLNFINYIPLKFLQIFDFSL
                     TRSVYSSTYLSFFYSIYFAVLTFLYPGYTWIKTRGRLGALVVVGVVFYGTYLVQYLSW
                     EPVGAATVVGVFTHYLVPLLALLPFVFSLNKEKILVKDMDIWVMTLMVGFIAGMMMLT
                     LVNCY"
     gene            complement(1552..>2118)
                     /locus_tag="PKK85_06200"
     CDS             complement(1552..>2118)
                     /locus_tag="PKK85_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNS25645.1"
                     /translation="QTAGIILMVACLQFILAVMLAETQYKFYSTSLNTLSDLGAVAGY
                     SAIIFNLSIVALGIMGLVVVYLISKSGSCPLFSACLAVSSLAAVGVGLFPTYTGTPHL
                     LFSGLVFLFASLAVIFSYRLGLNLPLVVLSLVLGLVSLLTLFSFLWMGMDSPLIHSLG
                     VGGAERLVAYPILGYLVGLGGYLTAGCR"
BASE COUNT          510 a          531 c          501 g          576 t
ORIGIN      
        1 ttgatattaa atcctgttca ctattaaaat taaaaaatct tacccgtgat gtgtgggatc
       61 ttagattttt catactgttg ttcctgggtt tcctggccct ctacattttc tccttattta
      121 aactagataa ctacctctat cccaaggcag agctaatcct cttggtggtg gtgacgctgc
      181 tgggaacgtt ctgcatcacc tactactcta aaaggaaagg tcagcttcat cgtgtggcgc
      241 tggttgtaat cttaagtttt ggtttggtta gtgttttctt aacaccactc ctggatgtgc
      301 tggatgaacg tgaacatttc acccgggcag aaatgacttc ccgaggagta attttcccgg
      361 aatatgatta ttcaactgga acctaccaga ccatcaaatc agtggaggat atgcacaacc
      421 gacacagtta ccctcatcta acagttttca acactgactt tgattcccgt aagattgatg
      481 ataacccggg ccagttaaag tcagcctttg tccagaaccc cttttttggg tatctggcac
      541 cggctgttgg aataaaggtg gctaagcttc tggatctgaa cgcggtttgg atgctctggc
      601 tggggaggct tttcaacctg atactgtacg ctctggtctc tgcttatgcc ataaagaaat
      661 cacccatatt taagatgcag ttaatggcag tggcttgttt acccctagcc atttactatg
      721 ctgcctctac tagcatcgat gccatgatca attcctttgc cctactggta attggatatt
      781 tcttctacat gtacaaatca gcagactatt ccctggattg gaggcatctc ctggtatttt
      841 tcaccctgtg cctggtctgt ggtttctgta aagtcaccta catggccctg tctctcctgg
      901 tcctggtggt gcccctatcc agattcaagt taaaaagata ttacctggcc gggggactgg
      961 gaatagtggg tttgctgttg ttaaccatat catggacttc ctacgtctta cccttatcct
     1021 ataactcctg gagggggaac agttaccttt taaggggggt taactccacc cagcaggtga
     1081 attatatgct gtctaaccca gcccagaccg gagtaatgtt cctcaatttc attaactata
     1141 tccccctgaa attcctgcaa atatttgatt tcagcctcac acgttccgtg tactcatcta
     1201 cttatctatc cttcttttat tccatctact ttgcagtgct aacattcctg tacccaggct
     1261 acacctggat aaaaactagg ggtagactgg gggccctggt ggtggttgga gtagttttct
     1321 atggcactta cctggtccag tacctatcct gggagccagt gggggcggcg actgtagtgg
     1381 gtgttttcac ccattacctg gtgccccttt tagccctctt acccttcgta ttcagcctta
     1441 acaaggaaaa aattctggtt aaagatatgg atatctgggt gatgaccctg atggtgggtt
     1501 tcattgccgg gatgatgatg ctgaccctgg tgaactgtta ctagaaaccc cttacctgca
     1561 cccggctgtg aggtacccgc ccaggcccac caggtaccct aggatggggt aggccaccag
     1621 gcgctcggct ccacccaccc ccaagctatg gatcagggga ctgtccatcc ccatccacag
     1681 gaagctgaat agtgttaaca ggctaaccag gcccagtaca agggataaca ccaccagggg
     1741 caggttcaaa cccaatcggt aggagaaaat aaccgccagg ctagcgaaaa gaaaaacgag
     1801 gcccgagaac aggagatggg gggtgccagt gtaggtgggg aagagtccca cccccaccgc
     1861 tgccagggaa gacactgcca ggcaggcgga gaacaggggg cagctgccac ttttagatat
     1921 caggtacacc accaccaatc ccatgatccc cagggcaacg atgctgaggt tgaagatgat
     1981 ggccgagtat ccagctactg ctcccaagtc actgagggtg ttcagggatg tgctgtagaa
     2041 tttgtactgg gtctcagcca gcatcacggc cagtataaac tggaggcacg ccaccatcaa
     2101 gattataccc gcagtctg
//
LOCUS       DAOQVW010000270         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_66137_length_2117_cov_3.445199, whole genome shotgun sequence.
ACCESSION   DAOQVW010000270 DAOQVW010000000
VERSION     DAOQVW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66137_length_2117_cov_3.445199"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PKK85_06205"
     CDS             <1..502
                     /locus_tag="PKK85_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25646.1"
                     /translation="DRQQGEPANPQSLPDLPDSVVIPDDPLGRTDLMVEIYETLFLAM
                     SIGASKIMGVSPARGLTKQFLPFNECKKLLEGVDVKSNAVIDFEALRVNAEKIKVEDR
                     EAVFLKDFSRIISVITDNYGKVMGYDAFRGMVRSEFRAINDSYGDAMDELGIKGKMHP
                     ELQKLL"
     gene            558..1523
                     /locus_tag="PKK85_06210"
     CDS             558..1523
                     /locus_tag="PKK85_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HNS25647.1"
                     /translation="MSGPWVEKYRPQTLDEVVGQEHIIDRLERYVKDKSMPNLMFTGP
                     AGVGKTTTALALVRAILGKYWKQNFLELNASDARGIDTVRTDIKNFCRLKAVGAPFRI
                     IFLDEVDNMTKDAQHALRREMEMYTKTSSFILSCNYSSKIIDPIQSRCAIFRFAPIKG
                     HHIIKRLEVICQAEDLNYTPGALESIVYFAEGDMRRAVNILQASASVGEEITEENIQE
                     VVSKAKPRDVRRIINMALDGDFMAARDLLREVMVVQGTSGEDMITQIYQEVSRMSMEG
                     LIEKNQYIFLVDHIGTYDYRIREGSNPRIQLEALLTKFLPKIKAD"
     gene            1524..>2117
                     /locus_tag="PKK85_06215"
     CDS             1524..>2117
                     /locus_tag="PKK85_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS25648.1"
                     /translation="MLWTEKYRPQTFKEVLGNSKAKEQIQDWLEGWMEGEPQKCLLLV
                     GPPGTGKTTLAHLVAEHFTEYLELNASDKRSQDALQRSVGEASASQSLFGPGLKLIIL
                     DEVDGLHGNEDRGGIRTINQIVKTSQHPVIMMANDLYSKRITTLKTRCQLVKINKIHT
                     NSILAMLKRICVKEGVEFEEHVLRTLAKRSRGDLRSAI"
BASE COUNT          589 a          480 c          561 g          487 t
ORIGIN      
        1 cgaccgacag caaggggaac cggcaaaccc acaatcacta cctgaccttc ctgatagtgt
       61 ggtgatccct gatgatcccc tgggaagaac tgacctcatg gtcgaaatct atgaaaccct
      121 gtttctggcc atgtccattg gcgccagtaa gatcatgggc gtgtctccag cccgggggct
      181 tactaaacag tttttaccat tcaatgagtg taaaaagctt ttagaaggtg ttgatgttaa
      241 aagtaacgct gttattgact ttgaagcatt gcgggttaat gctgaaaaga ttaaagtcga
      301 ggatcgggaa gctgttttcc ttaaagactt cagtcggatc atatcggtga tcaccgataa
      361 ttacggtaag gtaatgggct atgatgcctt ccggggaatg gtacggtccg aattcagggc
      421 tataaatgat tcttacggag atgctatgga tgaattgggg attaaaggaa aaatgcaccc
      481 cgaactccag aagttattgt agaagtccct gattgttctt tttaggaagt tgtggaatta
      541 ttgaaaaggt gaagtttatg agcggaccat gggtagagaa gtaccggcca cagaccctgg
      601 atgaagtggt gggtcaggaa cacatcattg atagactgga acgatatgtt aaggataaga
      661 gcatgccaaa cctcatgttc actggaccag caggggtggg gaaaaccacc actgccctgg
      721 cactggtcag ggccatcctg ggcaagtact ggaagcagaa cttcctggaa ctcaatgcct
      781 cagatgcccg tggtatcgac acagtccgga cggatattaa gaatttctgc cgattgaagg
      841 cagtgggggc acccttccgg atcattttcc tggacgaggt ggacaacatg accaaagatg
      901 cccagcacgc cctgagacgg gagatggaga tgtacaccaa aacatcctcc tttatcttgt
      961 cctgcaatta ctcctccaag atcatcgacc ctatccagtc ccgttgcgcc atttttcgtt
     1021 tcgcacccat taagggccat catatcataa aacggttgga ggtcatctgc caggctgagg
     1081 atcttaacta caccccaggt gcactggaaa gtattgtgta ctttgcggaa ggggatatgc
     1141 gtagggcggt gaatatattg caggcttcag cctcggtggg tgaggagatc accgaggaaa
     1201 atatccagga agtggtttcc aaggctaaac cacgggatgt gcgaaggata attaatatgg
     1261 ccctggatgg agacttcatg gccgcccggg acctgttaag agaggttatg gtggtgcagg
     1321 gtaccagtgg agaggatatg atcacccaga tctaccagga agtgtcccgc atgtccatgg
     1381 agggcttgat agagaagaat cagtacatat tcctggtgga tcatattgga acctatgatt
     1441 accgcattcg agaaggtagc aacccccgta tacaactgga agctctctta actaaattct
     1501 taccaaaaat aaaggcagat tagatgttat ggaccgaaaa ataccgacca caaaccttta
     1561 aggaagttct aggcaattcc aaggccaaag aacagatcca agattggttg gagggttgga
     1621 tggagggaga accccagaag tgcctgctgt tagttggacc tcccggtacg ggtaagacca
     1681 ccctggccca cttggtggcc gagcacttca ctgaatactt ggagctcaac gccagtgaca
     1741 aacgttccca ggatgcactg caacgtagcg tgggggaagc atcagcatcc cagtccctgt
     1801 ttggtccagg attgaagctc ataatcctag atgaggtgga tggactccat ggcaacgagg
     1861 atcgtggtgg tatacgcacc attaatcaga tcgtcaaaac cagccagcac cctgtgatca
     1921 tgatggccaa cgacctctac agtaaacgga tcaccaccct gaagacccgt tgccaacttg
     1981 taaagattaa caagatacac accaactcca tactggccat gctcaagagg atctgcgtca
     2041 aggagggtgt ggagtttgag gaacacgtac tccgcaccct ggccaagcgt tccagggggg
     2101 atttaagatc agccatc
//
LOCUS       DAOQVW010000271         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_66294_length_2114_cov_3.793103, whole genome shotgun sequence.
ACCESSION   DAOQVW010000271 DAOQVW010000000
VERSION     DAOQVW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66294_length_2114_cov_3.793103"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PKK85_06220"
     CDS             <1..938
                     /locus_tag="PKK85_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNS25649.1"
                     /translation="KIFPLVILTLLGLVFFILKPSHLISNLTPFAPQGWGGMGGALVL
                     IFWAYVGFELVTVPSDEILDAKRTIPRALVLGMVLIIVFYFLTNFVIVGSVPWQELST
                     SSAPLALAGKTIIGGLGAIILAIGALFSISGSDEAGILSSARIPYAMAGDGLFPHFFA
                     RVHPVYGTPYLSLLVQGVVTTIATLLGTISELIILSVFTLLLCYLVTCLSVFPLRKNY
                     EGGINLPRVVPILGVIISVYLMTQCSPIQIVTGVLLMLLGIPVYIKYSPGDEIKSVKR
                     EIKERRGLFDRELRWRERFLSHTLGHLASLMRRIR"
     gene            complement(985..>2114)
                     /locus_tag="PKK85_06225"
     CDS             complement(985..>2114)
                     /locus_tag="PKK85_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS25650.1"
                     /translation="HFLEVFKELRKPREELENFLEVKGVYLPDNPVEINLIQDMEQAW
                     KPVHQVIPETDEKRLIPAFGRSFLLEVSRGCTRGCRFCMAGCLYRPRREVPLEELLET
                     AQKTRKSTGLNKVALIGAAVSDHSRIEDLCQQLLEKDFQITLPSLRVESVTDQLLSIL
                     KDSGLKTVTLAPESTWNLRRVVNKSLTDEDIFAAVEKIFQHDLNVKLYFMVGLPTETD
                     KDILELVNLIRTLQKKAPRYSSLRVSVNPFIPKPHTPFQWHHFNYKEIKNKISSLNKE
                     FRSRYFKAESPRSSLIQYVLSVGGVEIGVVLERSLQHKLSLPDWQKLSPHWDLESDLP
                     WKNIQVGVSDEFLKSEYYNALKGDFTPWCETFGCQNCGACP"
BASE COUNT          461 a          558 c          542 g          553 t
ORIGIN      
        1 tgaagatctt ccccctggtg atcctcaccc tgctgggcct ggtgttcttc atcctcaaac
       61 cctcccattt aatatccaac ctaacaccct tcgcccccca gggctggggt ggtatgggag
      121 gtgccctggt cctgatattc tgggcctacg tcggattcga actggtaact gtaccctcgg
      181 atgagatact ggatgctaag agaaccattc cccgggccct ggtactgggg atggtcctca
      241 tcatagtctt ctacttttta accaacttcg taattgtggg gtcggtaccc tggcaggagt
      301 tatccaccag ttcagcaccc ctggccctgg caggaaaaac catcatcgga ggtttaggtg
      361 cgataatcct ggcaattggt gctttattct ccatatccgg atctgacgag gcgggaatat
      421 tatcatcagc ccggataccc tatgccatgg ctggtgatgg cctgtttccc cacttctttg
      481 ccagggtgca tccggtgtat ggcactccct acctatccct attggtgcag ggggtggtaa
      541 ccaccatcgc caccctcctg ggaaccataa gtgaactcat catcctctcg gtattcacac
      601 tgcttctatg ttacctggta acctgcctat cagtgttccc cctccgcaag aactatgagg
      661 gcggtattaa cctccccaga gttgtgccta tcttaggggt gatcatcagt gtttacctca
      721 tgacccagtg ctcccccatc cagatcgtga ctggggtcct actgatgctc ctgggaatac
      781 ctgtttacat taagtactca cctggtgatg agatcaaatc ggtgaaaagg gagataaagg
      841 agcgcagagg attattcgac cgggaactta ggtggaggga aagattcctc tcccacaccc
      901 taggccacct ggccagtctg atgaggagga tcaggtaaaa agctagtcgg agataaaata
      961 ttcctacctt gaaagaggga ggaatcaggg acaggcacca cagttctggc aaccgaaggt
     1021 ctcacaccag ggtgtgaagt cacctttcaa ggcattataa tactcacttt ttagaaactc
     1081 gtcactgact cccacctgga tgttcttcca gggaagatcg gattccagat cccagtgggg
     1141 agaaagtttc tgccagtccg gtagggataa cttgtgttgg agtgatcttt ccagaacaac
     1201 ccctatttcc actcccccca cggataaaac gtactggatg aggctgctgc ggggactttc
     1261 cgccttgaaa taacgggaac ggaactcctt gttcagggag ctgatcttgt tttttatctc
     1321 tttgtagttg aagtggtgcc actggaaggg ggtgtggggt ttgggtatga atggattcac
     1381 actcacccgg agggaactat acctcggcgc ctttttctgg agggttctga ttaaatttac
     1441 tagttccagt atatccttat cagtctcggt gggcaggccc accatgaagt acaacttgac
     1501 gtttaagtcg tgctggaaga ttttctcaac ggcagcaaag atgtcctcat cggtcaggga
     1561 tttgttgacc accctgcgga ggttccaggt ggactccggt gccagggtga ccgtcttcag
     1621 tccgctatct ttaagtatcg aaaggagctg gtctgttact gattccaccc gcagggaggg
     1681 cagggtgatc tggaaatcct tttccaaaag ttgttggcac aggtcctcta tccgggagtg
     1741 gtccgagact gccgccccta tgagggccac cttgtttagt cccgtggatt ttctggtctt
     1801 ctgtgctgtt tctaggagtt cctccagggg cacttccctg cggggacggt acaggcagcc
     1861 ggccatacag aaccggcagc ccctggtgca cccccgggac acctccagga ggaatgatct
     1921 tccaaaggcc gggatcaaac gtttctcatc tgtctcaggt ataacctggt gcaccggctt
     1981 ccaggcctgt tccatgtcct ggatgaggtt gatctccacc ggattatcgg gtagatacac
     2041 ccccttaact tccaggaaat tctccaactc ctcccgtggt tttctgagtt ctttaaatac
     2101 ctctaagaaa tggg
//
LOCUS       DAOQVW010000272         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_66436_length_2111_cov_3.745136, whole genome shotgun sequence.
ACCESSION   DAOQVW010000272 DAOQVW010000000
VERSION     DAOQVW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66436_length_2111_cov_3.745136"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /gene="lysS"
                     /locus_tag="PKK85_06230"
     CDS             <1..1104
                     /gene="lysS"
                     /locus_tag="PKK85_06230"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNS25651.1"
                     /translation="HPLKPDWLPYNPICSECGRVNTTYAYDFQGDSVQYRCQCGYNGE
                     MDITSGEGKLTWRVEWAARWKIFKVTCEPFGKDHAASGGSYDVSKKISTEIFNYRAPY
                     PVPYEWITLKGDAMSKSKGVFFTPGQWLEIGSPETLNYFLFRSKPLKHKDFNPEMPFL
                     DFIDQYDRIERIYYGEDEAASAKEEEKLKKIYEISMITPQKEIPFQPSYRFLTVAYQI
                     NQSPEDIYRILKANSQLPADMEELHFQELEDEDLKKFNERLDQVKNWLNLYAPKFVKF
                     QVQKEIPHVELNPAQEGFLAELADSLEKKNYSSEELHDEMYRILKKHDLKPQKAFQSI
                     YRVVLGQKQGPRAASFLLSLDKDFLIRRLRREA"
     gene            1134..1652
                     /locus_tag="PKK85_06235"
     CDS             1134..1652
                     /locus_tag="PKK85_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HNS25652.1"
                     /translation="MSPRLVEKGVLNPLADKEVGEIDLNPFPDDFERVALVDNTKPGA
                     NVILATLREALGNRIFIEVKKAAGAAATLNQLEKAASAELAILSLADCGSCTSWVVLD
                     AIRLEEMGIPTISIVSNRFAPFARELASTHGLDDLRLLEVEHPIAGLSVNEVEEKTLK
                     IIPALKYILQIP"
     gene            1670..>2111
                     /locus_tag="PKK85_06240"
     CDS             1670..>2111
                     /locus_tag="PKK85_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25653.1"
                     /translation="MTKKDQKMDFPENSCGCLLDDILNAEFYDTGTQGERILIPPDVE
                     KISHEFYLRHLTDGLPIIPPTRERVCKFLEYSIQEGEDVIAVLPPRQGRATVEKIAIN
                     AVMAGCLPSFMPVVEQTIRALGEEKFNLAGINATTHPVAICTIIN"
BASE COUNT          640 a          459 c          483 g          529 t
ORIGIN      
        1 caccccctga aacctgactg gctcccctac aatcccatat gcagtgaatg tggacgtgtt
       61 aacaccacct acgcctatga cttccaaggt gacagtgtac agtaccgttg ccaatgcggt
      121 tacaatggag aaatggatat cacctctggt gagggtaaac tgacctggag ggttgaatgg
      181 gcggcccggt ggaagatatt caaagtaacc tgcgaaccct ttggcaagga ccatgctgcc
      241 agtggaggat cctacgatgt tagcaaaaag atatcaacag agatattcaa ctatcgcgcc
      301 ccttatcccg tgccatatga gtggatcacc ctcaaagggg atgccatgtc aaaatccaaa
      361 ggagtgttct tcacccctgg ccaatggctg gagatcgggt cgccggaaac cctaaactac
      421 tttttattcc ggagtaaacc acttaaacac aaggatttca accctgaaat gccttttttg
      481 gattttatag accagtatga ccggattgag cgcatctact atggagagga tgaggcagcc
      541 agcgctaagg aggaggaaaa gctcaagaag atttatgaaa tttccatgat aaccccccag
      601 aaggaaattc cgttccaacc ctcctaccgt ttcctgaccg tggcttacca gatcaatcaa
      661 tccccggagg atatatacag gatacttaag gctaattcac aacttcctgc agatatggag
      721 gaactccatt tccaggaact ggaagatgag gatctgaaaa agttcaatga gcgtttggat
      781 caggttaaaa actggttaaa tctctacgcc cctaagtttg tgaagttcca ggtacagaaa
      841 gagattcccc acgtagaatt gaatcccgcc caggaagggt tcctggcaga gttggccgac
      901 tccctggaaa agaaaaatta ctcctccgaa gaattacacg atgaaatgta tcgcatactc
      961 aaaaaacatg atttaaaacc gcaaaaagca ttccaatcca tttatcgcgt tgttctcggt
     1021 caaaaacagg ggccaagagc ggcatcattt ttactatccc ttgataagga cttcctaatt
     1081 agacgattgc gaagggaagc ctagattccc aaaataaaaa ttgaagattt cttatgtcac
     1141 caaggttagt ggaaaaaggt gttttaaatc ccttagccga taaggaagtg ggtgaaatag
     1201 atttaaatcc ctttccagat gattttgaga gagtagcgct ggtggataac accaaaccag
     1261 gagcaaatgt tatactggct acattaaggg aagctctggg taaccgaatt ttcattgagg
     1321 ttaaaaaggc agcaggagca gcagcaactc ttaatcaact agaaaaagct gcttcagcag
     1381 agctggccat cctatcactg gctgattgtg gttcctgtac cagttgggtg gttctggatg
     1441 caattcgctt ggaggagatg ggaattccta ccatttccat cgtatcgaat cgtttcgccc
     1501 cttttgcccg ggaactggcc agtacccatg ggttagatga tttgcgcctt ttggaagtgg
     1561 aacatcccat tgcgggtctt tcagtaaatg aggtggagga aaaaacccta aaaataatcc
     1621 ctgcattgaa gtacatctta cagattccct aaaaaattgg tgcagataca tgactaagaa
     1681 agatcagaaa atggacttcc cagaaaatag ttgtggctgt ctcctggatg atattctaaa
     1741 tgcagagttt tatgatactg gaactcaagg ggaaagaata ttaatccctc ctgatgtcga
     1801 aaaaatcagt catgaattct atttacgcca tttaaccgat ggtttaccta tcataccccc
     1861 caccagagag cgggtctgta aatttttaga atattcaatc caagaggggg aggatgtgat
     1921 tgctgttctt cccccccgtc agggaagggc cacagtggag aaaattgcca taaatgcagt
     1981 tatggctggt tgtctaccca gtttcatgcc agtggtggag caaactataa gggcactggg
     2041 ggaagaaaag ttcaatttag ctggaataaa cgctactacc catccagtag ctatttgcac
     2101 cattataaat g
//
LOCUS       DAOQVW010000273         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_66592_length_2108_cov_3.789089, whole genome shotgun sequence.
ACCESSION   DAOQVW010000273 DAOQVW010000000
VERSION     DAOQVW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66592_length_2108_cov_3.789089"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..69
                     /locus_tag="PKK85_06245"
     CDS             4..69
                     /locus_tag="PKK85_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HNS25654.1"
                     /translation="MPIHARTLIWEVLSELESIRH"
     gene            complement(158..721)
                     /locus_tag="PKK85_06250"
     CDS             complement(158..721)
                     /locus_tag="PKK85_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNS25655.1"
                     /translation="MYQTKKKVAMASQYIYKQGLAPGKSSNLSIRKDDIVAITPTLTS
                     LRDVESESVVLVRVDGEIISEGLSPSSELELHLLIYQERSDVQGIVHTHSPYATGFAL
                     AGEKINLLEGFGPVNKSHLKMVDYAHPGSSKLAQLVRDSVRKEDVLIMESHGVLAVGS
                     SIDEAVLLADWVEKTAKTLFVARALKK"
     gene            complement(837..>2108)
                     /locus_tag="PKK85_06255"
     CDS             complement(837..>2108)
                     /locus_tag="PKK85_06255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HNS25656.1"
                     /translation="EFLEDKVELREPIIQSKLLPQEFDFQIIHDASKKSYTNGEIKDF
                     SSYFANRYHKLWQMLTKKKELQNVQPIKNAQRSEDVVEVVGMVNNIRNTKKNHKLMEI
                     EDETGNIPLLVHNDNHQLYEDAERIVKDQVVGVVGSRKGSLLMASQILNPGIPRIDEK
                     EMNFSAVFLSDTHIGSSTFLEEAFQRFLKWMRGDYGDEVQREIANDVKYLVIAGDIVD
                     GIGVYPGQDKELKIKDIYQQYEEAARLFGELSNVKIIIAPGNHDACRLAEPQPALPEE
                     YAPDLYQMKNVEFVSNPSLVSMDGMKTLIYHGRSFDDLAMTVKGLSHQNSDIIMKELL
                     EKRHLAPIYGERTPLASEIEDHLVIEDVPDVFHTGHVHINSYKKYKGIHLINSGTFQS
                     QTEFQKIYNIVPTCAQVPVLNRGSMKLLKFS"
BASE COUNT          546 a          476 c          387 g          699 t
ORIGIN      
        1 aacatcccca ttcatgcgcg gacccttata tgggaagttt taagtgaact tgaatctata
       61 aggcattaaa aatgttcata tacctagatt aatacatttt atagcagcat ataataattt
      121 atcaaagtcc atcaaattat tttttctttt ctccaaacta cttttttaaa gcacgggcta
      181 caaacaacgt ttttgcagtt ttttcaaccc aatctgccag cagaacagct tcatcaatgg
      241 aggaccccac tgccagtaca ccatgagatt ccatgatgag aacgtcctct tttctcacac
      301 tatcccttac tagttgggct agttttgaac ttccaggatg ggcataatcc accatcttca
      361 agtgtgactt gttaactggc ccaaaacctt ctaaaagatt tatcttctct cccgccaatg
      421 caaatccagt agcataaggg gagtgggtgt ggactattcc ttgcacatca ctacgttctt
      481 ggtagatcag aagatgcagt tccaactctg atgagggact aagcccttca ctgataattt
      541 caccatcaac tcttactagc accactgatt cactttctac atctcttaaa gaagttaagg
      601 tgggagttat agccactata tcatcctttc tgatactcag gttacttgat tttccaggtg
      661 ccagtccttg cttatagatg tactgggaag ccattgccac tttttttttg gtttgataca
      721 tggtacaacc acactacaac cggttattag taacaaaaaa tctagtgatg atttttattt
      781 attggtattt ttttctaaat taacagtcaa gatcctttat caagttattt aatcccctaa
      841 ctgaacttga gaagcttcat ggatccccga tttagcacgg gtacttgggc acaagtaggt
      901 acgatgttgt atatcttctg aaattctgtt tgggattgga aggttccact gttgatgagg
      961 tgaattccct tgtatttctt gtaactgttt atgtgcacat ggccggtgtg aaacacgtcg
     1021 ggcacatcct ctataaccag gtggtcttca atttcagaag ctagaggtgt tctttcacca
     1081 tatataggtg ctaggtgtcg tttttcaagc aattccttca tgattatatc ggagttctga
     1141 tggctaagtc cttttaccgt cattgccaaa tcatcgaagc ttctaccatg ataaatcaat
     1201 gttttcattc catccatact aaccagggag gggttgctta caaattccac gtttttcatc
     1261 tggtacaagt ctggtgcata ctcttctggc agagcgggtt gaggttcagc taatcggcag
     1321 gcatcgtgat tcccaggagc gataataatt ttaacgttgc tcaactctcc aaagaggcgg
     1381 gcggcttctt cgtactgctg gtaaatatct ttgatcttca gttccttatc ctgaccagga
     1441 taaactccaa ttccatctac gatgtcacct gcaattacca agtactttac atcgttggca
     1501 atttcacgtt gcacttcatc tccatagtcc cccctcatcc acttcaaaaa cctttggaag
     1561 gcttcttcca ggaaagttga actgcctatg tgggtgtctg atagaaacac agccgaaaag
     1621 ttcatttctt tttcatcaat tctgggtatt cccgggttta atatctgact ggccatcaat
     1681 aaacttccct ttctactacc aaccacccct accacctggt ccttaacaat gcgctcagca
     1741 tcctcataga gttgatggtt atcgttatgg accagaagag gaatgtttcc agtttcatcc
     1801 tcaatttcca ttaatttgtg gtttttcttg gtgttacgga tgttattaac catccccacc
     1861 acttccacca catcttcact tctctgggca ttttttatgg gctggacatt ttgcagctct
     1921 ttttttttgg tgagcatctg ccagagttta tgataacggt tggcaaagta ggaagaaaag
     1981 tctttaattt ccccgttggt gtaggacttc ttactggcat catgtattat ttgaaaatca
     2041 aattcctggg gtaagagttt actctggatt atgggttccc tcaactccac cttgtcttcc
     2101 aggaattc
//
LOCUS       DAOQVW010000274         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_67132_length_2097_cov_3.788932, whole genome shotgun sequence.
ACCESSION   DAOQVW010000274 DAOQVW010000000
VERSION     DAOQVW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67132_length_2097_cov_3.788932"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKK85_06260"
     CDS             complement(<1..272)
                     /locus_tag="PKK85_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25657.1"
                     /translation="MAMDPKETIIYAGVTFLFIMGIYTAFIGLYSMGVTWIVVAVFFL
                     AIFKQVRRPMKGVRQRKFIIMACALALFALGLYSFFQGEILIGASWL"
     gene            493..1671
                     /locus_tag="PKK85_06265"
     CDS             493..1671
                     /locus_tag="PKK85_06265"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNS25658.1"
                     /translation="MNTNEIIGLDKEYVMQTYGRQPIALKKGRGAVVWDVEGRSYIDC
                     VAGIAVNNLGHAHPRLVEAITQQAGKLIHTSNLYYTENQVKLAQLLAQISPHAKSFFC
                     NSGAEANEGAIKLARKYTGKGEIIAMDNSFHGRTITTITATGQKKYQKGFGPLTPGFV
                     HVPYADVNAVADAITDNTAAVLVEPVQGEGGVIIPPAGYLEDLKKLCHERKVLLIFDE
                     VQTGFGRTGEMFASQTFKVAPDITTLAKAIAGGFPMGAVLASDEVGSAFQPGDHAATF
                     GGGPLACAAALASVETIECEGLLRKSREDGKYFLDGLNQLWEEHGIVEDVRGVGMMLG
                     MELSIPCAKIVNEMREKGVLVNCAADVVLRFVPPLVITRKEIDTVINVLDDILARISD
                     "
     gene            1962..>2097
                     /locus_tag="PKK85_06270"
     CDS             1962..>2097
                     /locus_tag="PKK85_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNS25659.1"
                     /translation="MNLHYQTNDKGHLAIGGADALDLAQEYGTPLYVVDEMRVRENYR
                     R"
BASE COUNT          578 a          444 c          517 g          558 t
ORIGIN      
        1 agccaggatg ctccaatgag tatctctccc tgaaagaagc tgtataaacc cagtgcgaaa
       61 agggccaggg cacaggccat tataataaat ttacgttgac gaaccccctt cattggtctt
      121 ctcacttgtt tgaagatggc taaaaagaag actgccacta cgatccaggt tacgcccatg
      181 gaatagaggc ctatgaaagc agtgtagatt cccattataa acaagaaggt tactccagcg
      241 tagatgatcg tttctttggg gtccatggcc atccatgggt ttctccacta tttaatggtt
      301 tctagacccg ttgctggtga gttgatcact agagtttggg gttctctcca tgataggata
      361 gtttatagac tgcaagtagg ggtttgatgt ttattccacc atagattttt ggtgtctagt
      421 ttgaaaatta ttataaagta cacggtttaa taagaaatat agaagatttt agatttattt
      481 aaggtgaaga aaatgaacac taatgaaatc attggcctgg ataaagaata cgtcatgcag
      541 acctacgggc ggcaaccaat agctctgaaa aaaggcagag gggctgtggt ctgggacgtg
      601 gaaggtcgtt cttacattga ctgtgtggct ggtattgccg tgaacaactt gggccatgcc
      661 catccccgcc tagtggaggc cataacccag caggctggaa agctaatcca cacctccaac
      721 ctttactaca ccgaaaatca ggttaagcta gcccagcttc tggctcaaat ttcaccccat
      781 gctaaatctt tcttctgcaa cagtggagct gaggccaacg agggcgccat aaagttggcc
      841 cggaaataca ctggtaaggg tgagattata gctatggata acagttttca cggacggacc
      901 atcaccacca tcaccgctac tggtcagaaa aagtaccaaa aaggttttgg acctttaact
      961 cctggttttg tacatgttcc ttatgctgat gttaatgctg tggcagatgc catcaccgac
     1021 aacaccgccg ccgtgctggt ggaaccagtc cagggtgagg gtggggttat aattccacct
     1081 gcaggttatc tggaggattt aaaaaagtta tgccatgaaa ggaaggtttt attaatattt
     1141 gatgaagtac aaactggttt tggacgtacc ggagagatgt tcgccagcca gaccttcaaa
     1201 gttgctcctg acatcaccac cctggctaaa gccatcgccg gagggttccc catgggagcc
     1261 gtcctggcaa gtgacgaggt gggcagcgcc tttcaaccag gagaccatgc agctaccttt
     1321 ggaggaggtc cactggcttg tgcagctgca ttggcatctg ttgagaccat tgaatgtgaa
     1381 ggattacttc gaaagtccag agaagatggc aaatacttcc ttgatggact caaccagtta
     1441 tgggaagaac atggtatcgt ggaggatgtt cggggtgtgg gtatgatgct gggaatggag
     1501 ttaagtatcc cctgcgccaa gatcgttaat gaaatgcggg aaaagggtgt gctggtgaac
     1561 tgcgccgccg atgtcgtgtt aaggttcgtt cctccactgg ttatcacaag aaaagaaatt
     1621 gataccgtta taaatgtgct ggatgatatc ttggctcgta tatcagatta acttccagct
     1681 ggctcatttc tctttttacc acccattttt ttgtggacca gtaccccgct ccaagtaccc
     1741 cccagtaaca gatgtataat gtccagatct ctgaatatct cagagccgca tcaagaagta
     1801 caggatcttc agcgccctaa tatgttcagg tttacattgt taaggataag ctgacaggaa
     1861 tacgggttta gggccaagtc cgtaatatgt atctttttat attaaacagg acaaatccaa
     1921 tacattatag tattttatat ttcttcaatg gaggatttat catgaacctg cactatcaaa
     1981 ccaatgacaa aggacacttg gccattggcg gagcagatgc tctggatctg gcccaggaat
     2041 acggtacacc cttatatgtg gtggatgaga tgagggtgag ggagaactat cgccgcg
//
LOCUS       DAOQVW010000275         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_67450_length_2091_cov_3.619352, whole genome shotgun sequence.
ACCESSION   DAOQVW010000275 DAOQVW010000000
VERSION     DAOQVW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67450_length_2091_cov_3.619352"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKK85_06275"
     CDS             <1..225
                     /locus_tag="PKK85_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HNS25660.1"
                     /translation="TEIKNTEEIIVGVGAGAAIKKKVGDAQESIKNQKEELEGLLNRM
                     MGDLQNISEIIVQKSPEAEKLLQQLEGTQG"
     gene            247..1533
                     /gene="ftsY"
                     /locus_tag="PKK85_06280"
     CDS             247..1533
                     /gene="ftsY"
                     /locus_tag="PKK85_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNS25661.1"
                     /translation="MFDFLKKKFSGAVGKVSDKFSDEESLPGDETGTGKKMLPVEGET
                     VEKASGKEQNGSSLSAEDISSTDDSGFKIAEEEKTSRFAIFRRKSTHKEEVSGTDKEE
                     TVDKIVPEDIDETRTGPGSDKVKTKDTSLESEPGLFGFATHKTISQGDLEDVLFELEL
                     SLLEGDVALEVAEKIIKSVENDLVGRKIKRRSDVALHTQDALKKAISEILMVKGPNVM
                     ALAQEARKAGEPLKLMFVGINGTGKTTTISKLADYFVSKGFTPVLAASDTFRAGAIEQ
                     ITHHAENLKIKIIKHQKGADPAAVAYDAVEHARAQKKELVFIDTAGRMQTNTNLMDEM
                     KKIQRVVKPDLVIFVGDALTGNDVVEQATKFNDAVGLDGIILTKADADAKGGAALSIG
                     YVVQKPILFLGVGQGYKDIIEFKPDWMVEQIFGSDA"
     gene            complement(1609..>2091)
                     /locus_tag="PKK85_06285"
     CDS             complement(1609..>2091)
                     /locus_tag="PKK85_06285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS25662.1"
                     /translation="PQAHLTCLDLAENMIQNTRHKLAEYGEVDYVVGDLRDLDAVEEY
                     DAVVSSLAIHHLSRDEQRSFYSQIYTALKGGGVFYNADNLQSSSESLNQFYLEKWRKF
                     MLQYHSPKEVEAVWLARHHQEDHPQPLTDHLDWLKEAGFQDLDVVWKHYYFGVYGARK
                     "
BASE COUNT          655 a          450 c          514 g          472 t
ORIGIN      
        1 accgagataa aaaacactga agaaattata gtgggagtgg gagccggtgc tgccatcaaa
       61 aagaaggtcg gtgacgctca ggaaagtatt aaaaaccaga aagaggaact ggaaggcctc
      121 cttaatcgta tgatgggaga cttgcagaat atcagtgaaa taatcgtcca gaaaagtccc
      181 gaagcagaga aactactcca gcaactggaa gggactcaag gatagaccat caagagttag
      241 attccattgt tcgattttct aaaaaagaaa tttagcggag cagtaggtaa agtatccgat
      301 aaattttcag acgaagaaag tctccctgga gatgaaactg gcactggaaa aaagatgttg
      361 ccagtggagg gggagacagt agaaaaagct tccgggaagg aacagaatgg atctagttta
      421 tcagctgaag atatatcatc cactgatgac tccggcttta aaattgcaga ggaagaaaaa
      481 acctcccgtt ttgccatatt ccgccggaaa tcaacccata aagaagaagt atctgggaca
      541 gataaggaag aaactgttga taagatagtt ccagaagata tagatgaaac cagaactgga
      601 ccaggttctg ataaagtaaa aacaaaggac acatccctgg agagtgagcc aggcctgttt
      661 ggattcgcca cccacaaaac catatcccag ggggacctgg aagatgtgct atttgaactg
      721 gaactctccc tactggaggg cgatgtggca ttggaagtgg cagagaagat cataaaatcg
      781 gtggaaaacg atctggtggg gcgtaaaatt aaaagaagga gtgatgtcgc cctacatacc
      841 caggatgctc ttaaaaaagc catatcagag atcttaatgg ttaaaggtcc aaatgtcatg
      901 gctctggccc aggaagccag aaaagcaggt gaaccattaa aactaatgtt cgtgggcatc
      961 aatggtacag ggaaaaccac caccatttct aaacttgctg attattttgt ttcaaaagga
     1021 ttcacacccg ttctggctgc atcagacact ttcagggctg gtgctattga acaaatcact
     1081 catcacgccg aaaatttaaa gataaaaatt attaaacatc aaaagggtgc tgatcccgcg
     1141 gcagtggcct acgatgccgt ggaacatgcc cgggcccaga aaaaggaact ggtattcata
     1201 gacaccgccg ggaggatgca gaccaacacc aacctcatgg atgagatgaa gaaaatacag
     1261 agggtggtga agccggatct ggtcatcttc gtaggcgatg cattgactgg taacgatgtg
     1321 gtggaacagg ccaccaaatt caacgatgcc gtgggattgg atggtatcat actaaccaag
     1381 gctgatgctg atgctaaagg tggtgcagca ttatccattg gatatgtggt ccaaaaaccc
     1441 atactatttt tgggcgtggg ccagggatac aaggatatca tagaattcaa gccagattgg
     1501 atggtggagc agatcttcgg gtcagatgcc taataactat acatcacacc agaacctgta
     1561 acctatttac ccctggattt tgaaataata tttccaaggg cttatccact attttctagc
     1621 accgtacact ccgaagtagt aatgcttcca caccacatcc aggtcctgaa acccggcctc
     1681 cttcaaccag tccaagtggt ctgtgagggg ttggggatgg tcttcctggt ggtggcgggc
     1741 cagccagaca gcttccactt ctttggggct gtgatactgt aacatgaatt ttctccattt
     1801 ttccagataa aactggttca aactctcaga cgaggactga agattatcgg cattgtaaaa
     1861 cactccacca cccttcaggg ccgtgtaaat ctgggagtaa aatgaacgct gctcatcacg
     1921 ggacaggtga tgaatggcca gggaactcac cactgcgtca tattcttcca cagcatccag
     1981 atcacgcaga tctcccacca cataatccac ctccccatac tcggccagtt tgtgtctggt
     2041 gttctggatc atattctccg ccagatccag acaggtgagg tgagcctggg g
//
LOCUS       DAOQVW010000276         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_67927_length_2082_cov_3.558461, whole genome shotgun sequence.
ACCESSION   DAOQVW010000276 DAOQVW010000000
VERSION     DAOQVW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67927_length_2082_cov_3.558461"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKK85_06290"
     CDS             complement(<1..425)
                     /locus_tag="PKK85_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNS25663.1"
                     /translation="MFKKRRVNSHSGERIVLKTKPRFMVHSAGVFFKVIIVLLILYFF
                     RLVLEWIKNITNSLDAFIQLPLVEWTTYVLLIVILVLILGVAYDYLCWRQKKYTLTNH
                     RIIVQSGIIKRKKSYIHYDKIQDIVVSQSLVDRLLSAGD"
     gene            509..1237
                     /locus_tag="PKK85_06295"
     CDS             509..1237
                     /locus_tag="PKK85_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNS25664.1"
                     /translation="MIKRRVVVEESNILLTTDMENLDLAKFVIKERSLLKRYIHSHPE
                     FLCSLEPLKVEIAPQIVNEMAYASNLASVGPMAAVAGTISQLCLNYLLKQGSRYSIVD
                     NGGDVALKVNKDVTVGLFAGGSSLSGRLGFKVKHSLTPLGICTSSGTVGHSLSFGRSD
                     SVTVFAAKASVADALATALGNYAQGEEDGDAVQNCLSRAEELRDHFRGMVVIVGESAG
                     TIGKIPKMVETSKKVVLEDLFELY"
     gene            complement(1267..>2082)
                     /locus_tag="PKK85_06300"
     CDS             complement(1267..>2082)
                     /locus_tag="PKK85_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNS25665.1"
                     /translation="KKGSDIEKNHLLLKKDTVLTPDKIGVLSAIGMAYIPVYTQIKVG
                     IISTGNEIIPVGEDLDYGKVYDVNSQTISSAVHSCGCIPVGIKIIADDFKVLKNAISR
                     LMNEVDIIITSGGTSAGAGDVLRSVIEDMGEVLVHGISFKPGKPTLIGTLKGKEDKIL
                     IGLPGYPVAALVVFESLVAPFLRRNSGVSVSDESNTIQLKISRRFHPSRGRTHHLLVK
                     VRDDIAIPIQKDSGAITALADADGYVEIPKSTEILETGTLVHVKLFRRTTGLF"
BASE COUNT          605 a          441 c          410 g          626 t
ORIGIN      
        1 atatcccctg ctgataataa tctatccacc aaactttgtg aaaccacaat atcctgtatc
       61 ttatcgtaat ggatgtagct tttttttctt tttattattc cgctttgaac gatgatgcgg
      121 tgattggtca aggtatattt tttttgtcgc cagcacagat aatcatatgc cacccctaag
      181 attagtacta aaataactat taacaggaca taagttgtcc attccaccag aggcagttga
      241 ataaaggcat ccaagctatt tgtaatgttt ttaatccatt ccagtactaa acgaaagaag
      301 taaagaataa gaagtacgat tataacctta aaaaacaccc cggctgaatg taccatgaat
      361 cggggtttag tctttaaaac tattctttcc ccggaatgcg aatttactct tcttttttta
      421 aacatatcca ccacaatata agtcctatct ctactattct ttatggggcg tctaaaatat
      481 ttatgttctg gagccatatc catactaaat gatcaaaaga cgggtagtgg ttgaagagtc
      541 taacatactc ctcaccaccg atatggagaa tctggatctg gccaagtttg taataaagga
      601 acgttcccta ctaaaaaggt acatacatag ccatccagaa tttttatgta gcctcgagcc
      661 tctgaaagtg gaaattgccc ctcaaattgt aaatgaaatg gcgtatgcta gtaaccttgc
      721 aagtgtaggc cccatggcag cagtagctgg caccatatcc cagttatgcc ttaattatct
      781 tttaaaacag ggatcccgat acagtatcgt tgataatggt ggtgatgtgg ctttaaaggt
      841 caataaggat gttacagtag gtttattcgc tggaggatca tctttatctg gacgtctggg
      901 ttttaaagtt aagcacagcc taactcccct ggggatctgc acctcatcag gcacggtggg
      961 gcactcgctt agctttggta gatcagactc agtaactgtt tttgccgcaa aggccagtgt
     1021 agccgatgcc ctagccactg ctctgggtaa ttatgcccaa ggagaggaag atggtgatgc
     1081 agtgcaaaat tgcctttccc gggccgaaga actccgggat catttccggg ggatggtggt
     1141 aatcgtagga gaatcagccg gcaccatcgg gaagataccc aaaatggtgg aaacatctaa
     1201 gaaagtcgtc ttagaagacc tattcgagct ttattaattg ctattacata aatcctgtaa
     1261 aataattcaa aacagacccg ttgtacgccg aaatagtttc acatgcacca aagttccggt
     1321 ttccaggatt tctgtgcttt taggtatctc aacgtaacca tctgcatccg ccaaagcagt
     1381 gatggcacct gaatcctttt gaataggtat tgctatatca tcgcggactt ttaccagtag
     1441 atgatgggtt cgacctcggg aaggatgaaa gcgtctggat attttgagtt gtatggtatt
     1501 tgattcatca gacactgaaa ctcctgaatt tcgcctaaga aatggtgcta ctaatgattc
     1561 aaagactact aaagccgcaa ctggataacc aggtaaccca attaaaattt tatcttcttt
     1621 accctttaaa gtacctatta aagtgggttt tccaggtttg aatgatattc catggactaa
     1681 aacctccccc atatcttcga tcactgatcg aagcacatca ccagcaccgg cggatgttcc
     1741 ccctgaggtt ataattatat caacctcatt cattaacctg cttatggcat ttttcaggac
     1801 tttgaagtca tcagcaatta tcttaatacc tactgggatg catccacagg aatggacagc
     1861 cgaactaatg gtttgagagt taacatcgta cacctttcca tagtcaagat cctcacccac
     1921 cggaattatc tcgttgccag tggatatgat tccaaccttt atctgggtgt aaactggtat
     1981 atatgccatt ccaattgcac ttaaaactcc aattttgtct ggagtcaaga ccgtgtcttt
     2041 tttcaaaaga aggtgatttt tttctatatc agagcctttt tt
//
LOCUS       DAOQVW010000277         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_69124_length_2060_cov_3.166584, whole genome shotgun sequence.
ACCESSION   DAOQVW010000277 DAOQVW010000000
VERSION     DAOQVW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69124_length_2060_cov_3.166584"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /gene="rpiA"
                     /locus_tag="PKK85_06305"
     CDS             <1..462
                     /gene="rpiA"
                     /locus_tag="PKK85_06305"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HNS25666.1"
                     /translation="DEHRPDLAVDGADEVDPHLNLIKGGGAAHTREKIVDYAARTFLV
                     IVDEAKLVDQLGRFPVPVEVLPGAMSTVKEGLESMGGTAHLRMAHMKDGPVISDNNNF
                     ILDVQFESIPHPVHLEKELNNIPGVLENGIFTGAVDEVLAGSVDQVKSLKK"
     gene            501..893
                     /locus_tag="PKK85_06310"
     CDS             501..893
                     /locus_tag="PKK85_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25667.1"
                     /translation="MRLLTRKLLVAILVVGGILAAFGIYMSTHPMVQEATGSVAIVAP
                     EEEDTLVIPEEAHVPGNYTIPSQIMYRGNTYSVLKDLNFVGPVTPLCPYCGSNSTAQA
                     GSSSQGKYWFYYYQCHTCHRKFATYASR"
     gene            complement(984..1304)
                     /locus_tag="PKK85_06315"
     CDS             complement(984..1304)
                     /locus_tag="PKK85_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25668.1"
                     /translation="MIPFFLGSWLLTVTLEAVVLGLALKSKPAWQVLFYSALVNSLTL
                     PLAQYLYLHYLDNLVLMEAVVVLVEAPLIYLLLGVETRWALTLSVLANLVSALVGLLL
                     LTHH"
     gene            complement(1301..1903)
                     /locus_tag="PKK85_06320"
     CDS             complement(1301..1903)
                     /locus_tag="PKK85_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25669.1"
                     /translation="MVLVLVFVLSLTPVYGDVLEPGQKTVPIYYVLENMNQYPGYVFL
                     LHGDPSPDFMILNSSPFTFYKFSVASIYAVDKSQFNPEELMALNTTQVEGFFQNNTRL
                     LRSNHQLRSIYGTVKDTNPLDNATIMLKVVALDEDGLEIRKDRIIFGYHDGQSLSRSF
                     VEQNSTPASPRDDDNLYWYYLLPILAALAILLILLYRWYR"
BASE COUNT          546 a          491 c          563 g          460 t
ORIGIN      
        1 gatgagcacc ggccagacct ggcagtggat ggagccgatg aagtggaccc ccatctcaac
       61 ctcatcaagg ggggtggagc cgcccacacc cgggagaaga tcgtggatta cgctgccaga
      121 accttcctgg ttatcgtgga cgaagccaaa ttggtggacc agttgggaag gttcccggtg
      181 ccagtggagg tccttcccgg agccatgtcc acagttaagg aaggcctgga atccatgggt
      241 ggcacagccc atttacggat ggcccacatg aaggatgggc cggttataag tgacaacaac
      301 aacttcatcc tggatgtgca gtttgaaagc atcccccacc cggttcatct ggagaaggag
      361 ttaaacaaca tacccggggt cctggagaat ggcatattca ccggggcagt ggatgaagtc
      421 ctggctggct ccgtggatca ggttaaatca ttgaaaaaat agatatattg ctatctacta
      481 ttagggagat tgagtagttt atgcggctct taactaggaa attactggtg gccatcctag
      541 tggtaggggg gatactggca gcctttggta tatacatgag cacccacccc atggtacagg
      601 aagcaactgg ctccgtggcc attgtagccc cggaagagga ggacaccctg gtaatccccg
      661 aggaggccca tgtccctggg aactacacca tcccctccca gatcatgtac agggggaata
      721 cctacagcgt cctgaaggac ctgaattttg tgggaccagt cacaccactg tgtccctact
      781 gtggctccaa ctccacggcc caggctggtt cctcaagtca gggaaagtac tggttctact
      841 actaccaatg ccatacctgc caccgtaagt tcgccaccta cgccagtcgc tagtgaataa
      901 tgggaggaag cctctcataa attggtagtg ggcagtgttc actgctagaa ttatgtatct
      961 aactattttt aaaatgcagt tcttcagtgg tgggttagga ggagcagccc taccagggca
     1021 ctgaccaggt tagccagaac cgagagggtg agggcccagc gtgtctcaac ccccaggagc
     1081 aaatatatta agggggcttc caccaggacc acaactgcct ccatcagcac caagttatcc
     1141 agatagtgaa gataaaggta ctgggccaag ggtagtgtca aggagttaac cagagccgag
     1201 taaaacagca cctgccaggc tggtttactt tttaatgcca atccaagcac cactgcctcc
     1261 agggtcacgg tcaagagcca ggaacctaga aaaaaaggta tcatcggtac cagcggtaca
     1321 ggaggatcag taatatggcc agggcagcca ggatgggcag caggtagtac cagtacaagt
     1381 tgtcatcatc ccggggcgat gccggggtgc tgttctgctc cacaaagctc ctggataggg
     1441 attgtccatc gtggtagccg aagatgatcc ggtccttcct tatttcgagg ccatcttcat
     1501 ccagggccac caccttcagc atgatggtgg cattatccag gggattggtg tccttgacgg
     1561 tgccgtagat ggaccttaac tgatggttgc ttcttaatag acgggtgttg ttttggaaga
     1621 atccttccac ttgggtggtg ttcagggcca tcaactcctc cgggttaaac tgggatttgt
     1681 ccacggcgta gatggaggcc acactgaact tgtagaaggt gaagggggat gagttcagga
     1741 tcataaaatc aggggagggg tcaccatgga ggaggaagac gtagccaggg tactggttca
     1801 tattttccag cacataatat atgggcactg ttttctgacc cggctccagt acgtcaccat
     1861 aaaccggagt taaggataaa acaaaaacca ggactaaaac cataaccaga agatttccca
     1921 tattcataaa cctagatctg tagctgtttt cttataagat ttttccaaga aaagaaaaat
     1981 cctggactgt acttaggtgt atctacggat aaatgatgat aaaaggaagg ccaatgccat
     2041 gaagaggacg atggtggccc
//
LOCUS       DAOQVW010000278         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_69391_length_2055_cov_2.766500, whole genome shotgun sequence.
ACCESSION   DAOQVW010000278 DAOQVW010000000
VERSION     DAOQVW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69391_length_2055_cov_2.766500"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1189
                     /locus_tag="PKK85_06325"
     CDS             212..1189
                     /locus_tag="PKK85_06325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS25670.1"
                     /translation="MSNPIISIITPAYNLEGCLERSLNSVIKQTFPSLELVLVNDGST
                     DNTLNLATQVLENSGISHQILDQANMGVSQARNAGIRAARGDYLFFLDGDDYLKEDCL
                     EKMHQALIKHRAQTVCANYVKVTEEGEKLSIPQVMLPETSTAEYLIVLELSMAITFSF
                     CQLLYPHRLVTSEGISFNPGLCYGEDTDFALRVLAHLDRVAYLPEELIYYVQRGASST
                     RMNLLDRYDFIPALDGLKKYYQNHGLGGEVVDLIDQQRIPKSIMGNTNYLLDAGLSLE
                     EVVGELKKRKLLSRLKNFKPKGPRDYRFLAKTRLFTTSPWLYTHLRQVI"
     gene            1220..>2055
                     /locus_tag="PKK85_06330"
     CDS             1220..>2055
                     /locus_tag="PKK85_06330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS25671.1"
                     /translation="MDITVIIPNYNGKQYLKTCLESVRMQNHIKEVILVDNASTDGSV
                     EWIKNNYPGFTLLENKQNRGFAAAVNQGIRASQTPFILLLNTDVELEHNCTTHLRECV
                     KQDDRIFAASSRMVQYHHRERMDDAGDEYTILGWTKRVGYGKPLEKYDEAREVFSACG
                     GAALYQKKIIDMIGPLDERFFAYLEDVDLSYRARIHGYKVVYCPEAVVYHVGSASSGG
                     RYNQFKVPLSARNNVYLAYKNMPWPQLLVNLVFLVLGFSLKYLFFLHQGYGELYLQGV
                     KDG"
BASE COUNT          541 a          531 c          536 g          447 t
ORIGIN      
        1 gaaagggtcc tggacatctt ccaaaagcac cccttgatct tgtcctattt agaagaatta
       61 gtgatagaaa aagtaagaat tagaaagata gaagatatta aagaagcttt cgaacgagat
      121 gtaagtgctg atcttggtaa agtaatcctt atctgggata aataggaaaa aacagattgt
      181 gataacttat tctttctaga ggggtagaac aatgtcaaat cctataatca gcatcataac
      241 ccctgcttac aacctggagg gctgcctgga acgttcctta aactcggtta tcaagcagac
      301 ctttccctcc ctggagttgg tcctggtcaa cgatggcagc accgacaaca ccctcaacct
      361 agccacccag gtcctggaaa attccggcat aagccaccag atcctggatc aggccaacat
      421 gggggttagc caggcccgga acgccgggat cagagccgcc aggggtgatt acctgttctt
      481 cctggatggg gatgactacc tcaaggagga ttgcctggag aagatgcacc aggcccttat
      541 aaagcaccgt gcccagactg tgtgcgctaa ctacgtgaag gtcactgagg agggggagaa
      601 actgagcatc ccccaggtga tgctaccaga gacctccacc gctgagtacc tgatagtgct
      661 ggagctatcc atggccataa ccttcagttt ctgccagtta ctctatccac accgcctggt
      721 aacgagtgag ggtatctctt tcaaccctgg tctctgctac ggggaggaca ccgacttcgc
      781 cctgcgggtc ctagcccacc tggatagggt ggcctacctc ccggaggagc tcatctacta
      841 cgtgcagcgg ggtgcctcct ccacccggat gaacctcctg gataggtacg acttcatacc
      901 tgccctggat gggcttaaaa agtactacca gaaccatggc ctgggagggg aggtggtgga
      961 tttaatcgac cagcaaagga tacccaagag tataatggga aacaccaact acctattgga
     1021 tgcaggcctc tccctggaag aggtagtggg ggaattaaaa aagcgcaagc tcctatcccg
     1081 tttgaagaat tttaagccaa agggtccacg ggactaccgg ttcctggcca agaccagatt
     1141 gttcaccacc tcaccctggc tctacaccca cctgcgccag gtaatatagg ataaagataa
     1201 ggcccaataa gtgaaattta tggatataac cgtcatcatc cctaactaca atggaaagca
     1261 gtaccttaaa acctgcctgg aatccgtccg gatgcagaac catattaaag aggttatcct
     1321 ggtggataac gcctccaccg atggtagtgt ggagtggatt aaaaataact acccaggatt
     1381 cacgctcctg gaaaataaac aaaacagggg cttcgccgcg gcggtgaacc aggggatcag
     1441 ggccagccag accccattca tcctcctcct caacacagat gtggagttgg agcacaactg
     1501 caccacccat ctccgggagt gtgtaaagca ggatgatagg atcttcgctg cctcatcacg
     1561 tatggtgcag taccaccacc gggagaggat ggatgatgcc ggggacgagt acaccatcct
     1621 gggctggacc aagagggtgg ggtatggtaa gccccttgaa aagtacgatg aagcccggga
     1681 ggtcttcagt gcctgtggag gagcagcttt gtaccagaag aagatcatag atatgatcgg
     1741 ccccctggat gagaggttct tcgcctacct ggaggatgtg gacctcagct accgggcccg
     1801 tatccatggc tacaaagttg tttactgccc ggaggctgtg gtgtaccacg tgggcagcgc
     1861 ctccagtggg ggccgctaca accagttcaa ggtaccctta tcagcccgta acaacgtgta
     1921 cctggcctac aagaacatgc cctggcccca gttactggta aatctcgtct tccttgtctt
     1981 gggcttttcc ctgaagtacc tgttcttttt acaccagggc tacggtgaac tctacctgca
     2041 gggagtgaag gatgg
//
LOCUS       DAOQVW010000279         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_69592_length_2051_cov_3.459920, whole genome shotgun sequence.
ACCESSION   DAOQVW010000279 DAOQVW010000000
VERSION     DAOQVW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69592_length_2051_cov_3.459920"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /gene="mtxX"
                     /locus_tag="PKK85_06335"
     CDS             <1..476
                     /gene="mtxX"
                     /locus_tag="PKK85_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HNS25672.1"
                     /translation="FLLAPVGIDEGDTLERKVQIISQVAEFLKKIGIKPKIGILSGGR
                     PQDRGRSHKIDASLDEAEELTRITMDKYPVKHHFILIEEALAEGANLILAPDGICGNL
                     IFRSLVLVGSGKSFGAVTLGIDEIFIDTSRSQNKEGYLRALKLAAYLVNLRNRIE"
     gene            494..1261
                     /gene="uppS"
                     /locus_tag="PKK85_06340"
     CDS             494..1261
                     /gene="uppS"
                     /locus_tag="PKK85_06340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNS25673.1"
                     /translation="MEILKPIYRLYEWYISRNLRPENLPKHVAIIMDGNRRFTKVQGN
                     LNTIEGHKKGVDTLEKVLDWCIDLGIEIVTVYAFSTENFHRPAHEVQGLMQLFKKNFE
                     EITNNQKIHKNQVQVRAVGKLELLPEDVRAAINKAEESTANYNQRRLNIAIGYDGRME
                     IVDAIKKIVKDVEVGKLDVDDIDENIVNRNLYTAGVEDPNLIIRTSGEERLSGFLLWQ
                     SSYSELYFSNSLWPALRKVDFLRALRSYQERERRFGV"
     gene            1267..2025
                     /locus_tag="PKK85_06345"
     CDS             1267..2025
                     /locus_tag="PKK85_06345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNS25674.1"
                     /translation="MIDAHCHVDFKEFNRTRDQVMHRAQKKVKAIINSGASLGGNRRT
                     LKLAQDYPGFLFPALGFHPHYAHKTEKSVIEDVLKEIKSNLHRAVALGETGLDFHNLN
                     SEEIRKKQEKLFRIFLDIASEEEMPLVVHARDAEKKALEMSREFEGIPKIIFHCYGGD
                     LETAQQIMDEGHLISLSTLVCFSTHHQELARELPLSHLLTETDSPYLSPFKGKVNEPS
                     FVEETVKTIANLKLMDVEEVASITEKNAQEVFRI"
BASE COUNT          664 a          387 c          472 g          528 t
ORIGIN      
        1 aattcctcct ggctcctgtg gggatagatg aaggggatac actggaacgg aaggttcaaa
       61 ttataagtca ggtagctgaa tttttaaaaa agataggaat aaaacctaaa attggcatct
      121 tatcaggtgg gaggccgcaa gacaggggaa gaagccataa aatagatgct tcacttgatg
      181 aagcagaaga acttaccaga ataacaatgg ataaataccc agttaaacat cattttatac
      241 taatcgaaga agcattggcc gaaggtgcta atctcatttt agcccccgat ggtatctgtg
      301 gaaatttgat attccgcagc ctggttttag tgggctctgg aaaaagtttc ggagcagtta
      361 ccctgggaat agatgaaata ttcatcgaca catccagatc tcaaaataaa gaaggatatc
      421 taagggcttt aaaattagca gcctatttag tcaatctaag gaacagaatt gaataattta
      481 ataggggcta gtaatggaaa ttctcaaacc aatttataga ttgtacgaat ggtatatttc
      541 cagaaatctg cgtccggaaa acttgcctaa acacgttgct attataatgg atggtaaccg
      601 tagatttacc aaggtccaag gaaatcttaa caccattgaa ggccataaaa aaggggtgga
      661 tactctggag aaggttctgg attggtgtat cgatctggga atcgaaatcg ttactgtgta
      721 cgcattttcc accgaaaatt tccatagacc cgctcatgaa gtgcagggac tgatgcaact
      781 ttttaagaaa aattttgagg aaataaccaa caaccagaag atccataaaa atcaggtaca
      841 agttagggcg gtgggaaagc tggaactact gcctgaggat gtaagagctg ccataaacaa
      901 ggcagaagaa agcacagcca actataatca gcgccgtttg aatattgcca ttggctacga
      961 tggtcgcatg gaaattgtgg atgccataaa aaaaatcgtc aaagatgtgg aggtaggaaa
     1021 actggatgtt gatgacatag atgagaacat cgtgaaccga aacctttaca ctgcgggtgt
     1081 ggaagaccct aacctcatca taagaaccag tggtgaggaa agactaagtg gatttctttt
     1141 atggcaatca tcctattctg aactttattt cagcaatagt ttatggccgg cactgcgtaa
     1201 agtagatttt ttaagagctt taagatccta tcaggagcgg gaaagacgtt ttggagtgta
     1261 agatgtgtga ttgatgctca ctgtcatgtt gattttaagg agtttaacag gacccgtgac
     1321 caggttatgc accgggctca gaagaaggta aaggccatta taaattctgg tgccagcttg
     1381 ggagggaacc gtcgtactct gaaactggcc caggattatc ctggttttct cttccccgcc
     1441 cttgggtttc acccccatta tgctcataaa acagaaaaat cagtaataga agatgtttta
     1501 aaggaaatta aatcaaatct acaccgggcg gttgctctgg gcgagacagg tctagatttt
     1561 cataatctga acagtgaaga aattcgaaag aaacaggaaa agctcttcag aattttcctg
     1621 gatatagcat ccgaagagga gatgcccctt gttgttcacg cccgtgatgc cgagaaaaaa
     1681 gccttggaaa tgtctaggga atttgaagga ataccaaaga tcatattcca ttgttatgga
     1741 ggggacttag aaactgccca gcagataatg gacgagggtc acctcatctc cctatccacc
     1801 ttggtgtgtt tttcaaccca ccaccaggaa ctggcccgcg aattacccct atcccatctt
     1861 ttaacagaaa ctgacagccc ctatctttct ccattcaagg gcaaagttaa cgaaccttct
     1921 tttgtcgaag aaaccgttaa aactatagca aatttaaagt taatggatgt ggaggaagtg
     1981 gcgtccataa ctgagaagaa tgctcaggaa gtcttccgga tttagaggta gaaaataggt
     2041 cccccccctt t
//
LOCUS       DAOQVW010000280         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_69610_length_2051_cov_2.551102, whole genome shotgun sequence.
ACCESSION   DAOQVW010000280 DAOQVW010000000
VERSION     DAOQVW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69610_length_2051_cov_2.551102"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKK85_06350"
     CDS             complement(<1..466)
                     /locus_tag="PKK85_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25675.1"
                     /translation="MTSSTSLSLKKYSSEFKLSPSKHDLIKDHLKKLEEGQFKSETKN
                     YLYFYDIILHQLLGYDREENILFDEKEEEGKGKSEFVLRSLEGKFMVVELKDQTTDLD
                     KPQTRKNDKRTPVDQAFDYAQHTGDIEWIMVSNFDEFRLYNWHQKGKFISFRT"
     gene            complement(629..1210)
                     /locus_tag="PKK85_06355"
     CDS             complement(629..1210)
                     /locus_tag="PKK85_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS25676.1"
                     /translation="MLGKKLYSVRESYWIFYQGIRTLPYMFKARRKKELSSQFISRIM
                     LAVTEVNECAICSYAHTKRAMETGMTNDEIQDMLNGILDNVPAEEVAGVMFAQHYADS
                     RGNPTQEAWERVVEIYGLSRAQGVLGAIRMIMVGNTYGIAFSSFFNRFRGRSDPRSGI
                     LYEMGLLLSAVIVVPVSVVHALVSGLVKRPLIH"
     gene            complement(1244..1328)
                     /locus_tag="PKK85_06360"
     tRNA            complement(1244..1328)
                     /locus_tag="PKK85_06360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1292..1294),aa:Ser,seq:tga)
     gene            complement(1467..1775)
                     /gene="rpsJ"
                     /locus_tag="PKK85_06365"
     CDS             complement(1467..1775)
                     /gene="rpsJ"
                     /locus_tag="PKK85_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNS25677.1"
                     /translation="MNKARIKLTGTDPEKLAYVCDQLKRIAERTGVDLSGPIPLPTKK
                     LVVPSRKSPDGEGKATWEKWELRIHKRLVGIEADERAMRQVMKVNVPDNVSIEIELRS
                     "
     gene            complement(1832..2033)
                     /locus_tag="PKK85_06370"
                     /pseudo
     CDS             complement(1832..2033)
                     /locus_tag="PKK85_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876684.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-alpha"
BASE COUNT          519 a          500 c          436 g          596 t
ORIGIN      
        1 cggttctaaa tgatatgaac ttacccttct ggtgccaatt atagagccgg aattcatcga
       61 aattggagac catgatccac tcgatatctc ccgtgtgctg ggcataatca aaggcttggt
      121 ccacgggggt tcgtttatca ttcttccgag tttggggttt gtccaggtca gtggtctggt
      181 cctttaactc caccaccatg aacttcccct caagggatct tagaacaaac tcgcttttac
      241 ccttcccttc ctcttccttc tcatcgaaga ggatgttctc ctcccggtcg tagcctagaa
      301 gctggtggag gattatatca taaaaataga ggtaattttt ggtttctgac ttgaactggc
      361 cctcttccag cttttttaga tgatccttta tgaggtcgtg cttggaggga gatagtttaa
      421 actcagagga gtatttcttc aaagagagac tggttgaaga ggtcattctt cataaaatca
      481 aatttattta tgttgttgaa tattatagcc tttcggttga agagaaacaa ttgcttgata
      541 gctttctata aattccaata acttccattg aataattcag tatctaaatc ttttataaac
      601 tgatttatcg cacaatctta tcttaaaatc aatgaataag gggcctttta accaaccccg
      661 aaaccaacgc atgaaccaca gaaaccggaa ccactataac cgcactcaaa agcaatccca
      721 tctcataaag tatgccactc cggggatccg accggcccct aaacctatta aaaaacgaac
      781 taaacgcaat cccatatgta ttaccgacca taatcatgcg gatagccccc agaacaccct
      841 gtgccctgga caaaccatat atctccacaa cccgctccca agcctcttgg gtaggatttc
      901 cccgggagtc agcataatgc tgggcgaaca tcaccccagc tacctcctca gccgggacgt
      961 tgtccaggat cccgttaagc atgtcctgga tctcatcatt ggtcattcca gtctccatgg
     1021 cccttttggt gtgggcgtag gagcagatgg cgcattcatt cacctctgtc acagccagca
     1081 tgatcctcga aatgaattgg gagcttaatt ccttttttct tctagcctta aacatgtacg
     1141 gcagggttct gatcccctgg tagaagatcc agtaggattc ccggaccgag tagagttttt
     1201 tgcccaacat tacatcaaaa cagtagatta tataaaataa gtacgccggg acagggattt
     1261 gaaccctgga ggagccatgc tccacgggat ctcaagtccc gcgccttacc tggctaggct
     1321 atcccggcaa ataatatttc tatttcatga ataatttaat caaaattcct ttttttgatc
     1381 caaccctgat gggtgggggg aatgatttcc cagttaccaa aaagagtatg gtgttatttg
     1441 tttagaggct gtttgtttca gcctttctag cttcgaagtt ctatttcgat gcttacgttg
     1501 tcaggcacgt tgaccttcat gacctgacgc atggcccgtt catcggcctc gatacccact
     1561 aggcgtttat gaatccttaa ttcccacttt tcccaagtgg cttttccttc accatctggt
     1621 gatttgcgac tgggaaccac cagtttcttg gtgggtaagg gtatgggtcc ggatagatcc
     1681 acaccagtcc tctctgcaat ccgtttaagt tggtcgcaga cgtaggccag tttttcgggg
     1741 tcggtgccgg ttaacttgat tctggctttg ttcatgttcc tcctccataa aaagaaaaaa
     1801 agagggctaa gatgccctcc ttagtagata tttacttggc tggtaccagg tcaatgcaca
     1861 taccagcggc cacggtctga cccatgtccc ggatggcgaa tcgacccatg tgtggtattt
     1921 cactgatctt ttcgattacc ataggtttgg taggtttaac cactaaaagc ggcgtccccg
     1981 gtcttcagga agtctggatt ttcttcctta acctgaccag tggtggggtc cagttaagga
     2041 agaaaatcca g
//
LOCUS       DAOQVW010000281         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_69819_length_2047_cov_3.713353, whole genome shotgun sequence.
ACCESSION   DAOQVW010000281 DAOQVW010000000
VERSION     DAOQVW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69819_length_2047_cov_3.713353"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PKK85_06375"
     CDS             complement(<1..1122)
                     /locus_tag="PKK85_06375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS25678.1"
                     /translation="MKYDVVVIGGRVGGSTASLFASKNGLDVLMIEKRQEIGTPVQCA
                     GGTFRSTFDTLEMQPSPKYVCAEIYGGIICSPDGNRVKLKGKEVGGYILERKAFDKHL
                     AIESARAGTDVMVKTMVTDLIREDEKVCGVVAKHMGKTMEIKADLVIAADGVESQIAP
                     LAGLNTQKTPYDLGSCAQYEMVGVDVDLHYIEFHLGENLAPGGYLWVFPKGEGVANVG
                     VGVRNSPHHAYHYLQKFTSQLSATPVELNVGGVPLTGMVDPIYTDGFLVVGDAAGQVN
                     ALSGGGIHTTVECAKIAGEVAAESVERNDASASFLKRYEKLCRITVGKDLKKSLKYRR
                     VFDKLRDDDFNVITKFLDGYDLESISKLSMMSLIKEHPHL"
     gene            complement(1119..1301)
                     /locus_tag="PKK85_06380"
     CDS             complement(1119..1301)
                     /locus_tag="PKK85_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25679.1"
                     /translation="MPKIERLPDCCGYCGVCIAVCPQKCLKLSDNILVINEGCENCGY
                     CLQVCPLGAIKKEAPE"
     gene            1590..1672
                     /locus_tag="PKK85_06385"
     tRNA            1590..1672
                     /locus_tag="PKK85_06385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Leu,seq:taa)
     gene            1694..1766
                     /locus_tag="PKK85_06390"
     tRNA            1694..1766
                     /locus_tag="PKK85_06390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1727..1729,aa:Thr,seq:ggt)
BASE COUNT          548 a          477 c          400 g          622 t
ORIGIN      
        1 aagatgggga tgttctttaa tgagactcat catcgacaat ttagatatcg actccagatc
       61 atacccatct aggaatttag ttattacatt gaagtcatca tctcttaatt tatcaaatac
      121 acgacgatac ttaagggatt tttttaggtc ttttccaact gtgatcctgc atagtttctc
      181 gtatcttttc agaaaactgg ccgaggcatc atttctctcc acagattctg ctgccacttc
      241 acctgcaatt ttagcacact ccacagtagt gtgtattcca ccacctgaga gagcgttaac
      301 ctgccctgct gcatccccta ccaccagaaa accgtcggtg tagatggggt caaccatccc
      361 tgtgagggga acaccaccca cgttgagttc tacaggggtg gcagatagtt gtgatgtgaa
      421 tttttggagg taatgatatg cgtggtgggg tgaattccta acacccactc ctacatttgc
      481 cactccctcc cctttgggaa atacccacag atagccaccc ggagccaggt tctctcccaa
      541 gtgaaattct atgtaatgga gatccacatc cacacccacc atttcgtact gggcacagga
      601 acccagatca tagggagttt tttgggtgtt aagccctgcc aatggggcaa tttgggattc
      661 aacaccatcg gcggctatta ccaggtccgc tttaatttcc atggtttttc ccatgtgctt
      721 ggccaccact ccacagactt tttcatcctc ccttatcaga tcagtaacca tggttttaac
      781 cataacatca gtcccagccc gtgctgattc aatggccagg tgtttatcaa atgcttttct
      841 ttctaatatg tatcccccta cctctttgcc ttttaattta acccggttac catctgggga
      901 acatatgatc cctccatata tctcggcaca aacatattta ggtgagggct gcatttcaag
      961 ggtgtcaaag gtgctgcgaa atgttcctcc agcacactgg acgggagttc ctatctcctg
     1021 acgtttttca atcatgagca cgtccaggcc atttttagat gcaaatagcg aggcggttga
     1081 cccgcccact cttcctccaa tgaccaccac atcgtacttc attccggagc ctccttttta
     1141 atggcgccca ggggacatac ttgaagacaa tatccgcagt tttcgcagcc ttcattgatc
     1201 accagaatgt tatcgctaag tttcaaacat ttttgagggc aaaccgctat gcagactccg
     1261 caatatccac aacaatcagg aagtctttct attttaggca ccccacaatt acactatgag
     1321 aaatatattc attgataggt atggctatga ttcttatata aattactaat tagttcaact
     1381 cctctttgtt gtatcatagg gattactaag catataatat tctataaact ccacagaaca
     1441 ttttttaaca cttttaaaga ttaatttaaa tttttaatac cataaaatcc atgaattaat
     1501 ggtggatgct gtttaattaa gtggaaatct gttcccgctc caacctaacc gttaagttaa
     1561 tatatgatat tgcaccaagt tcgttgtatg caggggtgcc cgagcggcca aagggggagg
     1621 acttaagatc ctctggcgta ggccttcgag ggttcgaatc ccttcccctg cactgtagtt
     1681 agttttaatt actgccctag tggctcagtc ggtagagcga taccttggta aggtataggt
     1741 cgggggttcg attcccccct agggctttca aatgtatatt taaccttaaa gttacaattc
     1801 caccttatat attgtttgtg aaagcctttt attttactga aagttatttt actctgggga
     1861 ttgttaattg gcccccataa ttgtgctttc taaagggggg gtgaaaatga tactccatat
     1921 ccatccagag ttcatgatcg ggcaatggtg actcatactt tttgaggagt aagaagtcat
     1981 tagcaagatc cttagaatag acaataatgg agttaaagat ccgaaaatta taggaaccaa
     2041 ttcataa
//
LOCUS       DAOQVW010000282         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_69841_length_2047_cov_2.698795, whole genome shotgun sequence.
ACCESSION   DAOQVW010000282 DAOQVW010000000
VERSION     DAOQVW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69841_length_2047_cov_2.698795"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKK85_06395"
     CDS             complement(<1..548)
                     /locus_tag="PKK85_06395"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNS25680.1"
                     /translation="MIQMFLPIPLLATIIAILASVYASYTDITKGIIPNRLTFPLIGV
                     GMVLNGIYAILIGDLWYLILGLIITGVIFGLGYIFWKMGAWAGGDVKLFTALAALLPF
                     YPAVVSYQVFSHTFPMEAAYPFPLTVIINSILSMLPFLLIYVLFVAMRTKPHLLGELL
                     SPIKEWQKNLVTTLLITSAVTIS"
     gene            complement(558..2045)
                     /gene="pyrG"
                     /locus_tag="PKK85_06400"
     CDS             complement(558..2045)
                     /gene="pyrG"
                     /locus_tag="PKK85_06400"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HNS25681.1"
                     /translation="MKIDPYLNWDSGTMSPYQHGEVFVTCDGMETDLDLGHYERFLDV
                     DLLGESNITTGKVYDSVIKRERRGDYLGSCVQIIPHITDQIKSMIRQIARDSNSQVVL
                     VELGGTVGDIEGQPFLEALRQLRNEEGHDNVMFVHVTYVPYLRAAGEFKTKPTQHSTK
                     ELRSMGINPDMIICRSELAINDALKRKIAHFCDVDREAVINCPDMSSIYEVPLVLNQE
                     KVGEYVVDRIKLTAEPQDLSEWKDVVKALKKDDEVVSVGIVGKYVELEDAYTSIREAL
                     KHAAAHLGVKVNIEYIQSEESLEDNKVRHLDSLLIPGGFGERGIGGKIDAVRYSLENE
                     VPLFGICLGMHCMVIEYARLHGMENAHSTEFDPETPYPVIDLMKEQKKIKDMGGTMRL
                     GSYPCHLREGTEAISAYQTTDVEERHRHRFEVNNEYREELENRGLLVSGASPDDFLVE
                     IVELPEHPWFLGCQFHPEFKSRPNRAHPLFVSFLEAAIKSRVKRA"
BASE COUNT          539 a          509 c          455 g          544 t
ORIGIN      
        1 gagatagtca cggcactagt tattaacagg gtggttacca gatttttctg ccactcttta
       61 attggagaca gtaattcacc caacaagtgt ggcttagtcc tcatggcaac gaacaatacg
      121 tagatgagta aaaaaggcag catggatagg atgctgttga tgattacggt gagtgggaat
      181 ggataggcag cttccatggg aaaggtgtgg ctaaaaactt gatagctaac tactgccggg
      241 taaaatggga gtaaagcagc aagggcagtg aagagtttca catcaccacc agcccaagcc
      301 cccatcttcc agaaaatgta tcccaaaccg aatataactc cggttataat aagtcccagt
      361 atcaggtacc acagatcccc aattaaaatg gcataaatcc cattaagaac catccctacg
      421 cctattaggg gaaaagttag gcggttggga attattccct tggtaatatc agtgtaactg
      481 gcatagacag atgccaggat ggctataata gtagctaaaa gtggtatggg gaggaacatt
      541 tggatcatta aaaacttcta agcacgtttt actctacttt taattgcagc ctctaagaat
      601 gatacaaaca gcggatgggc cctgttaggc cgggatttga actcagggtg gaactgacaa
      661 cccaaaaacc agggatgttc aggtaactcc acgatctcca ccaggaaatc atcaggcgaa
      721 gcacccgaaa ccaggagacc cctgttttcc agttcttcac ggtattcatt gttcacttca
      781 aaacgatgac ggtgtctttc ttccacatca gtggtctggt atgcgctgat ggcctcggtg
      841 ccctcccgga ggtggcaggg ataggaaccc agacgcatgg taccacccat gtctttgatc
      901 ttcttctgct ccttcataag atcaataacc ggatatggag tttctgggtc aaattcggta
      961 ctgtgagcgt tttccatacc atgcaaacgg gcatattcta ttaccataca atgcataccc
     1021 aggcagatac cgaacaatgg aacttcattt tctagggagt agcgtactgc atcaatttta
     1081 ccccctattc ctctctctcc aaatccccca gggattaata aggaatctaa atgacggacc
     1141 ttattatcct ccaggctttc ctcggattga atatactcaa tatttacttt aactcccaga
     1201 tgggcggcgg catgtttcaa ggcctcccgg atgctggtgt aggcatcctc cagctccaca
     1261 tatttaccaa ctatacccac actcactact tcatcatcct ttttcagggc cttcaccaca
     1321 tccttccatt cggaaagatc ttgtggctct gcagtgagtt taatacgatc aaccacatat
     1381 tcacctacct tttcctggtt tagaaccagt ggcacttcat agatggagct catatccgga
     1441 caattaataa ctgcttcccg gtccacgtca cagaagtggg cgattttcct tttcagggcg
     1501 tcgttgatgg ccagctcgga acggcagatg atcatgtcag ggttgatacc catactcctc
     1561 agttccttgg tgctgtgctg ggttggtttg gttttaaact ctccagccgc acgcagatat
     1621 ggcacgtagg tcacgtggac gaacataacg ttatcatggc cctcctcatt cctgagttgc
     1681 ctgagggctt ctaggaaggg ctggccttca atgtctccca ctgtacctcc cagttccacc
     1741 agcactacct gggagttact gtcccgggct atctgtcgga tcatggattt gatctggtcg
     1801 gtgatgtggg gtatgatctg cacacaggat cccagataat cccctcttct ctcccgcttg
     1861 ataacggagt cataaacctt accagtggtg atgttggatt ctcccaagag atctacatcc
     1921 aggaaacgtt cgtagtgacc cagatccagg tcagtttcca ttccatcaca ggtcacaaaa
     1981 acctccccat gctgataggg actcatggtc cctgaatccc aatttaggta gggatcaatc
     2041 tttatgg
//
LOCUS       DAOQVW010000283         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_69981_length_2044_cov_3.229764, whole genome shotgun sequence.
ACCESSION   DAOQVW010000283 DAOQVW010000000
VERSION     DAOQVW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69981_length_2044_cov_3.229764"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PKK85_06405"
     CDS             <1..680
                     /locus_tag="PKK85_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNS25682.1"
                     /translation="LLLSGSPFMVQISAFCFGLIAVGLTYTIGGSFRGSKTLIMVLCG
                     IAISALFSAFISITKYLADPYDKLPAIVYWLMGSLSNTSLEDIYLILIPMIIGFSVLL
                     LIRWRINILAMGDEEAQSMGIDVSKLRMVIIICCTLLTAAAVSVSGIIGWVGLVIPHV
                     TRMLVGPDYKKLLPATICTGGFFLLLVDDVARNLTQIEIPLGILTALIGAPFFLYLLR
                     KGNEGWS"
     gene            671..1453
                     /locus_tag="PKK85_06410"
     CDS             671..1453
                     /locus_tag="PKK85_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS25683.1"
                     /translation="MVMKSHIKVVDASFAYNGSGNVFENITFSIEKGEVLCILGPNGT
                     GKTTLIKCLNRLLKLNTGNIFLKDKDIYSFNSKDLARQIGYIPQAHNPVFPFKVLDVV
                     LMGRAPHLTSLSSPSEKDLVIAKKSLEKLNIDHMADKPYTELSGGEKQLVFFARVLTQ
                     KPDILLLDEPTSHLDFGNQMRTLNIIDKMANEGFTVIMSSHFPDHAFISADKVAIMKD
                     CDLIDYGTPDEVITEQNLEKAYDIQVNIMDLEEGRKICIPLG"
     gene            1498..>2044
                     /locus_tag="PKK85_06415"
     CDS             1498..>2044
                     /locus_tag="PKK85_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNS25684.1"
                     /translation="MGDIKVYLDKGKEFHGDICPGIVIGTRIALAGLRELGMDPDQHN
                     RDLIVYVEIDRCMADAVQAVTGITMGHRTLKYKDYGKFAATFLDLSSGKAVRISATDN
                     PREKPDEGNPNMEGVVQKLIHAPESELLIIEEVDVDIREHDVPGFPKYKVYCEECGDK
                     VLDRREVIVNGQTLCKACAEGP"
BASE COUNT          624 a          365 c          432 g          623 t
ORIGIN      
        1 cacttctttt gtctggcagc cctttcatgg tacaaatatc tgcattttgt tttggtctca
       61 ttgcagtcgg ccttacatac accattggcg gtagtttcag agggtccaaa actttaataa
      121 tggtcctctg tggaatagcc atatctgcat tattctcagc ttttatatct ataactaagt
      181 atttagcaga cccttatgat aaattacccg ctattgtata ctggttaatg ggaagtctct
      241 ctaatacatc gttagaagat atttatctaa ttttaattcc tatgataatt ggattttcag
      301 ttctcttact aattcgttgg agaattaaca tcctggccat gggagatgaa gaagcgcaat
      361 ccatgggtat tgatgtttca aaattaagga tggttattat tatttgttgc acccttttaa
      421 cagcagcagc agtgagtgtc agcggcatta taggttgggt tggcttagtt attcctcatg
      481 taacccggat gctggttgga cccgactata aaaaactatt acccgccact atatgtacag
      541 ggggtttttt ccttctttta gtagatgatg tggccaggaa cttaactcag atcgaaattc
      601 ccctgggaat attaaccgcc cttatagggg cgcctttctt tttatatctt cttagaaaag
      661 gaaatgaggg atggtcatga aaagtcatat caaagtagtt gatgctagtt ttgcctataa
      721 tggcagtgga aatgtttttg aaaatatcac tttctccatt gaaaaaggag aagttttgtg
      781 tattcttgga ccaaatggta cgggaaaaac taccctaatc aaatgtttaa atcgtctttt
      841 aaagcttaat actggtaata tattcttaaa agacaaggat atttactctt ttaacagtaa
      901 agatcttgcc cgccaaatcg gttacatacc ccaggcccat aaccctgttt ttccttttaa
      961 agttttagac gtggttttaa tgggtcgtgc cccccatcta acatctttgt cttcgccatc
     1021 agagaaggat ttggtaattg ccaagaaatc cctggaaaaa cttaacattg accacatggc
     1081 cgataagccc tacaccgaat taagtggggg ggaaaaacag ttggtattct ttgcacgcgt
     1141 tttaactcaa aaacctgata ttttacttct agatgaaccc acatcccatt tggactttgg
     1201 aaatcaaatg cgaacactga atattatcga taaaatggca aatgaaggtt ttacagtaat
     1261 aatgtcctct catttcccgg accatgcatt tatatctgca gacaaggtag ctataatgaa
     1321 agattgtgat ttaatcgatt atgggacacc agatgaagtg ataaccgaac aaaacctgga
     1381 aaaagcctat gatatccaag ttaatataat ggatttggaa gaggggcgta agatctgcat
     1441 tcctctcgga taatttatgt tcatttaaaa aaatcaatga aaccatgagg taatagtatg
     1501 ggtgatataa aagtctatct tgataaagga aaagaattcc atggggatat ttgcccagga
     1561 atagtaatag ggaccaggat cgctctggcc ggattgagag aactgggaat ggatcctgac
     1621 cagcataatc gtgatttgat tgtttacgtg gaaatagatc gttgcatggc agatgcagtt
     1681 caagctgtca ctgggattac catggggcat agaacactta agtacaagga ctatggcaaa
     1741 ttcgcggcca catttctgga tctgtcatct ggaaaagctg tccgaatatc tgcaacagac
     1801 aatccacgtg aaaaaccaga tgaaggtaac ccaaacatgg agggggtggt gcaaaagctt
     1861 attcatgctc ctgaatcgga attattgata attgaggaag ttgatgttga tattagggaa
     1921 catgacgtac ccggatttcc aaaatacaaa gtatattgtg aagaatgtgg agataaggtc
     1981 ttggatcgta gggaagtcat tgttaacgga caaacactct gcaaagcttg tgctgaaggt
     2041 cctt
//
LOCUS       DAOQVW010000284         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_70131_length_2041_cov_3.955690, whole genome shotgun sequence.
ACCESSION   DAOQVW010000284 DAOQVW010000000
VERSION     DAOQVW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70131_length_2041_cov_3.955690"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..405
                     /locus_tag="PKK85_06420"
     CDS             28..405
                     /locus_tag="PKK85_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HNS25685.1"
                     /translation="MKLKILPTHLREKKRYLAFEAISQLPLKREDVISLLWDTSLHLY
                     GVCGTSKFDLWLVKTWNCPFTGDYRIRGIIRCRRESLNEVRAILPTVTRFNGKKVVFY
                     TRGISGTINSAVKKFIKLKEEDE"
     gene            483..1241
                     /gene="psmA"
                     /locus_tag="PKK85_06425"
     CDS             483..1241
                     /gene="psmA"
                     /locus_tag="PKK85_06425"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HNS25686.1"
                     /translation="MQPLAGAAGYDKGISIFSPDGRLFQVEYAREAVKRGTTSLGVKS
                     SEGVVLVVDKRPTSTLVEPQSIEKIFQVDDHIGAATSGLVADARALIEKARLESQINK
                     ITYNEPIAVEILAKKICDMKQMYTQHGGVRPFGSALIIGGVNDNGSRLFETDPSGALI
                     EYKATAIGAGRAIAMEVFEKNYAEDITLADAIELALDAIYEATEGKTTNESVEIAVIE
                     EKTHSYRKLSDDEMSEHVEELLIRKSKEDEIEEE"
     gene            1245..1940
                     /locus_tag="PKK85_06430"
     CDS             1245..1940
                     /locus_tag="PKK85_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HNS25687.1"
                     /translation="MVALEDAVIARLEYYGERFEVLVDPDLASDFKRGESINIDEILA
                     VEEVFKDAKKGDKASEEAMIKAFQTADPLEVAAAIIRKGNVQLTAQQRRDMQEEKRRS
                     IVAKITREAINPQTKLPHPARRIEIAMEEAKIHVDPFKSVDEQVVTVLKAIRTKIPIR
                     FEKVRVAIRIPGEATGKVYGVIPEYGETKKEEWQQDGSWIAVVEIPGGLQESFYQKLS
                     EITHGQVETRLMK"
BASE COUNT          688 a          368 c          506 g          479 t
ORIGIN      
        1 tgtccaacca atctgataag aggatccatg aagttaaaga tacttcccac ccatcttcga
       61 gagaagaaaa gatacttggc ctttgaggca atatcccaac taccgctgaa acgggaggat
      121 gtgatttccc tattgtggga cacatccctg catctatatg gggtatgtgg gaccagtaaa
      181 tttgacttat ggcttgttaa aacctggaat tgccctttta caggtgatta tagaatacga
      241 ggtataatca gatgccgaag agaatcatta aatgaggtta gagcaatttt acccactgta
      301 actagattta atgggaaaaa agtggttttt tacaccaggg gaatttcagg taccattaac
      361 tctgcagtaa aaaagtttat taaattaaag gaagaagatg aatgaatgga tagttttatt
      421 cttaccgaaa ccaaatattt ggtaaggttc atttcttcat tcaaaataga gaggtataaa
      481 ccatgcaacc attagcagga gcagcaggat acgataaagg tatatctatt tttagccctg
      541 atgggagatt atttcaagtt gaatatgcca gagaagctgt taaaagaggt acaacttcat
      601 tgggggtaaa gtcatcggaa ggtgtagtac tggtggtgga taaacgaccc acaagtacac
      661 tagtagaacc ccagtctata gaaaaaatat tccaggtaga tgaccatatt ggggctgcga
      721 cctcgggtct ggtggctgat gctcgggccc ttatagagaa agcccgatta gaatcacaga
      781 ttaacaagat aacctacaat gaacccatag ccgtggagat cctggctaaa aagatctgtg
      841 acatgaagca gatgtacacc cagcatggag gagtcaggcc ctttgggtcg gccctgatca
      901 taggtggtgt caatgacaac gggtcccgtc tattcgaaac agaccccagc ggtgctctta
      961 tagaatataa agccactgct ataggggcgg gaagggcaat agctatggaa gtcttcgaaa
     1021 aaaattacgc cgaagatata acacttgctg atgctatcga actggcatta gatgcgattt
     1081 acgaagcaac tgagggtaaa acaaccaatg aaagtgtgga aatcgccgtt attgaggaaa
     1141 aaacccacag ttatcgcaaa ctaagtgatg atgagatgtc ggagcatgtg gaagaacttc
     1201 tcatcagaaa gtccaaggag gacgagatag aagaggaata aagcatggtt gcccttgaag
     1261 atgcagttat agcccgatta gaatattacg gggagcgatt tgaagttctg gtagatccag
     1321 atctggcatc tgattttaaa aggggagaat ccatcaacat tgatgaaata ctggccgtgg
     1381 aagaggtttt taaagacgcc aaaaaagggg ataaggcttc agaagaagct atgattaagg
     1441 ccttccagac tgcagacccc ctggaggtgg ctgctgccat tatccgaaaa ggaaatgtgc
     1501 aactcacagc ccaacaaaga agggatatgc aggaggaaaa gaggagaagt atagtggcta
     1561 aaataaccag agaagccata aatccccaga ctaaacttcc ccatcctgct agaaggatcg
     1621 aaatagccat ggaagaagct aaaattcatg ttgatccctt caaaagcgtg gatgaacagg
     1681 ttgtcacggt tttaaaggcc attcgcacca agattcccat aaggtttgaa aaggtgaggg
     1741 tggctatcag gatacctgga gaagccactg gaaaagtcta tggcgtaata cccgaatacg
     1801 gagaaaccaa aaaagaagaa tggcagcaag atggctcctg gatagctgtg gtggagatcc
     1861 ccggtggttt acaggagagt ttctaccaaa aactgagcga aattacccat ggacaggtgg
     1921 aaactagatt aatgaaataa acttcttttt aagcctttta aaggaggcac aaagtgatat
     1981 tagtagaaga taaagatata gtggttccag gggagattct ggccgaagga gaatactacg
     2041 c
//
LOCUS       DAOQVW010000285         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_70157_length_2041_cov_2.593656, whole genome shotgun sequence.
ACCESSION   DAOQVW010000285 DAOQVW010000000
VERSION     DAOQVW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70157_length_2041_cov_2.593656"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKK85_06435"
     CDS             <1..304
                     /locus_tag="PKK85_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25688.1"
                     /translation="EILVLLSPEHATALGKGGWTKKDVKNYLHQNMVVPVKLGDRGGR
                     RLDEQWVEGDEVLITRSPEDVVLAVAGGPGRHTMIAPGFGSGSQSVTISLNQNNPS"
     gene            complement(323..799)
                     /locus_tag="PKK85_06440"
     CDS             complement(323..799)
                     /locus_tag="PKK85_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNS25689.1"
                     /translation="MNVEQDHQFMQDAIKEAYKSLKEGGLPIGAVLVKNGHIIGRGHN
                     QLLQKGSVILHGEMSCLESAGRLRGSDYRNCTLYTTLSPCPMCSGILILYQIPRVVVG
                     ESSTLQGPEWLLEENGVEVINLALPECQHLLEDYISKNPDIWGDEMERVGYTTDLK"
     gene            complement(893..>1021)
                     /locus_tag="PKK85_06445"
     CDS             complement(893..>1021)
                     /locus_tag="PKK85_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25690.1"
                     /translation="KEVEWYDRITGYVQQVGRAHNASGGWNAGKMKELGDRKRYEP"
     assembly_gap    1022..1031
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1032..2041)
                     /gene="nrdD"
                     /locus_tag="PKK85_06450"
                     /pseudo
     CDS             complement(1032..2041)
                     /gene="nrdD"
                     /locus_tag="PKK85_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036338.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
BASE COUNT          530 a          488 c          450 g          563 t
ORIGIN      
        1 tgaaattctg gtgctcctaa gcccggaaca tgccactgcc ctgggaaaag ggggctggac
       61 aaaaaaggac gttaagaatt atctgcacca gaatatggtg gttccagtga aactgggaga
      121 tcgaggcgga cggagattgg atgaacaatg ggtagaggga gatgaagttc ttataacccg
      181 tagcccagag gatgtggtcc tggctgtggc tggggggcca gggcgacaca ccatgattgc
      241 acctggcttt ggatccggat cccaatccgt aaccatctct ttaaatcaga ataacccaag
      301 ttaataaaag gaaattgcta atttatttta aatccgtagt gtaacccacc ctctccatct
      361 catcacccca tatatcggga tttttggaga tatagtcctc taataagtgt tgacactcag
      421 gtagggctag attaatcact tccacaccat tctcttccag aagccattcc gggccctgca
      481 gggtggagct ctcacccacc actaccctgg ggatctggta gaggattagt atcccactgc
      541 acataggaca tggggataat gtagtgtaaa gggtgcaatt ccgataatca gaccccctca
      601 atctcccagc actttcaagg cagctcatct ccccatgcag aattacagaa cctttctgca
      661 ggagttgatt atgccctctt cctattatgt ggccattttt taccagaacc gcgccaatag
      721 gcagtccacc ttcttttaaa ctcttatatg cttctttaat ggcatcctgc atgaactggt
      781 gatcttgctc aacattcaat atgatacacc gtaaaatgat ttattatatt tattccaaga
      841 ccaggaatta gcgaaaaaag acaagtgagt ttaaaaaaat atgaaaaaag aatcaaggtt
      901 cgtaacgttt cctatcccct aattccttca ttttcccggc attccagcca ccagaggcat
      961 tatgagctcg gcctacttgc tggacgtagc cggtgattct gtcataccat tcgacttctt
     1021 tnnnnnnnnn nttttgttcg ccgcatgatg cacattgctg ttgcagccct ttcatgaggg
     1081 ttttgcattt aatgcagaag ctcatggcgg aactgtaggc ccagaatcct atgtttgatt
     1141 ttcgagctat tttatcagtc aagctcatca gggcctgggg atcagagtaa gcttcaccca
     1201 tgaatgcatg gaagatatgt ccccccaagg ttcgactgtg gaactgttcc tcaactctta
     1261 ttctgtcgat gagatttata tcagaactca ccggtacatg agaagagttg gtgtagtaag
     1321 cagcttcggg atctccctgt gttaaggctt ccttaccgaa tttctcccgg tccaacatgg
     1381 caaaacggta ggcagtggac tcggccggag tctgtattat ggtccagcgc aaccccgtgt
     1441 ccttttttag ttcctgagcc ctttgattca tataatcaac gactttaagt ccaaatttaa
     1501 gtgaatcagg gtcttctatt ccacttccac aatggaattg cagcatttca ttcaaaccca
     1561 cgaatccgaa tgtcatggtt gagttctcaa cccggtagta gcgctcacca tccagttcct
     1621 gggtaaggaa gggtaacata ttgtagtcat ccagacaggc tagagcctgt ttacggcgca
     1681 gcatcaatac ttcctcggat ggcggaggta agaatccagg tactcaaata tttcatcctc
     1741 atcccgggat tggtaggcca atctaggcag gttaagtgtt atgtaggcca gatttccagt
     1801 acggaaacag tctttatccc attctcctgt ccagttatcc cccagggatg ttcggcatcc
     1861 catgtagttg gccagatttc cacgataact ggggagcatg tttatgaagt aagcggtgcc
     1921 gtacttggca cacaactggt gcaccagttc caggtcctca gcaaattcat ctttcaggac
     1981 ctccttcctt aaaccataga tggtatttgg gaataaatgc ggtttaccat ctgaatcgcc
     2041 t
//
LOCUS       DAOQVW010000286         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_70251_length_2039_cov_2.888105, whole genome shotgun sequence.
ACCESSION   DAOQVW010000286 DAOQVW010000000
VERSION     DAOQVW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70251_length_2039_cov_2.888105"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKK85_06455"
     CDS             complement(<1..506)
                     /locus_tag="PKK85_06455"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNS25691.1"
                     /translation="MELELSQFYRVLAPRTTIIITTINQKGEINAAPFSFTMPVSVNP
                     PLLAVASAPTHHTYQNLQESGEFVVNLPGEEILEQLWITGESFPEGVNELEKAGLTNE
                     DSLIVDAPGIKECVARLECKVVSDRECGDHQLVVGEVVRVDTRDDILVEGLLHVERSK
                     PVLHLGGK"
     gene            complement(507..>2039)
                     /locus_tag="PKK85_06460"
     CDS             complement(507..>2039)
                     /locus_tag="PKK85_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS25692.1"
                     /translation="EGEITIGDLTVKHDSPAYYSRKLREATAIHLQRSFGLWPESTVQ
                     NVVRKLYGVKYGDEGLTDFEHANDEFMEEAMELLHLVGLGDKAEHFAPVLSGGEKQRL
                     IMARQLAKKPEILLLDEPATMSCPKTKQEILDAIKNINQELGITVLLVSHLPEVHRYL
                     SHKLILMENGHVKDEGKTDEVINKFLSGMDEEISGRDSQDVGSTLIRVENLARRFYLL
                     KGGHVLSLEDVDFEVKEGEILSLIGPSGAGKTVLMRMMAGLDQPDRGKVSYKLDGNWV
                     DMHQPGIERMTIRRQMGFMHQEFSLTHHATIRDQIAHRLGVKNEMVVGAAKKKAEELG
                     ISDQVLDILYQLTDLPENEAKYRLEKIGLSPDVLDELFPSFPEKAVRRYAKPIFEALD
                     LPLEILDRRSYELSGGQKVRATIALVLATQPRILYLDEPFGDLDPLTLRTVSNSLKRI
                     NQEFNTTFIMVSHHVDFIQELATRAIMMEDGRLVDQGDTRTLCEEFIDRCHAKYLEKE
                     AV"
BASE COUNT          397 a          567 c          534 g          541 t
ORIGIN      
        1 tccttcccac ccaggtggag gacaggtttg ctgcgctcca catggagcaa tccctccact
       61 aggatatcat cccgggtgtc tacccggacc acttccccca ccaccagttg gtgatcacca
      121 cattccctgt cagagaccac tttacattct aaccgggcca cgcattcctt aataccaggg
      181 gcatcaacga tcaacgaatc ttcattggtt aagcccgcct tttccaattc gttcacaccc
      241 tcggggaagc tttcaccggt gatccatagc tgctccagga tctcctctcc agggaggttc
      301 accacgaatt caccactttc ctgtaggttc tggtaggtgt ggtgggtggg agccgaggcc
      361 actgccagga ggggagggtt cacagataca ggcatggtga aggagaaggg agccgcgttt
      421 atctccccct tctggttgat ggtggtgatg atgatggtgg tgcggggggc tagtacccgg
      481 tagaactgtg ataactccag ttccatctac accgcctctt tttcaaggta cttggcatgg
      541 cacctgtcaa tgaactcttc acagagagtc cgggtgtcgc cctggtccac caagcggcca
      601 tcctccatca taatggccct ggtggccagt tcctggatga agtccacgtg gtggctgacc
      661 atgatgaagg tggtgttgaa ttcctggttt attcgtttca ggctgttgga aacagtccgc
      721 aatgtcaagg gatccagatc tccgaatggc tcgtctaggt agagtattct tggttgggtg
      781 gccagtacca gggctatggt ggcccggacc ttctgtcccc cggatagttc gtaggaacgc
      841 cggtccagta tttccagggg aaggtccagt gcctcgaaga tgggcttcgc atagcggcgc
      901 actgccttct ccgggaagct ggggaagagt tcatctaaga cgtcagggga gagtccgatc
      961 ttctccagcc ggtacttggc ctcgttttct ggtaggtcag tgagttggta cagtatgtcc
     1021 aggacctggt cggatatccc caattcctcg gctttcttct tggcagcgcc tactaccatc
     1081 tcgtttttca cccccaagcg gtgggcgatc tggtccctta tggtggcgtg gtgggttaaa
     1141 ctgaattcct ggtgcatgaa tcccatctgc cggcgtatgg tcatcctttc aattcccggc
     1201 tggtgcatat ccacccagtt cccatccagt ttgtaggaca cttttcccct gtcgggctgg
     1261 tctaatccgg ccatcatacg cattaacacg gttttaccag cgccgctggg gccgatgagg
     1321 cttaatattt cgccctcctt aacctcaaaa tccacgtcct ctagggatag cacgtgccct
     1381 cccttcagca ggtagaacct ccgggccagg ttttccaccc tgatcagggt agaacccaca
     1441 tcctggctgt ccctaccaga tatttcttca tccatacccg agaggaattt atttataact
     1501 tcatcagtct ttccctcatc tttgacatgg ccgttttcca ttagtatcag tttatgggag
     1561 aggtaacggt gcacctcagg caggtgggat accaggagta cagtgatgcc cagctcctgg
     1621 ttgatgtttt ttatagcatc cagtatctcc tgtttggtct tgggacagga catggtggcg
     1681 ggttcatcca ggagcagtat ttcaggcttc ttcgcgagtt gccgggccat tatgagtctc
     1741 tgtttttccc ctccactcag taccggggcg aagtgctccg ccttatcacc caggcccacc
     1801 aggtgcaaaa gctccatggc ctcctccatg aactcgtcat tggcatgttc aaagtctgtt
     1861 aagccttcat caccatattt aacgccgtat aatttacgca ccacgttctg taccgtggac
     1921 tctggccata agccgaagga tcgttgcagg tggatggcgg tggcctcccg gagtttacgg
     1981 gagtagtagg ccggggagtc gtgttttact gtgaggtccc ctatggttat ctccccctc
//
LOCUS       DAOQVW010000287         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_71186_length_2022_cov_3.521607, whole genome shotgun sequence.
ACCESSION   DAOQVW010000287 DAOQVW010000000
VERSION     DAOQVW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71186_length_2022_cov_3.521607"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..869)
                     /gene="dapB"
                     /locus_tag="PKK85_06465"
     CDS             complement(45..869)
                     /gene="dapB"
                     /locus_tag="PKK85_06465"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNS25693.1"
                     /translation="MIDVAVTGAGGRMGSKIIKSILQQDDMRLVAAIEAPHTPLEGKD
                     VGEIIGVGKIGVKIRGAENLPEVLREKKPQVLVDFTIAGAAMETIKTTAQAGVNLVVG
                     TTGFSDEQLEIIKGIIEENYVAAVISSNMAIGVNVFFKMVEELAGLLPDYDVEIIEAH
                     HHHKKDAPSGTAVTAFELIAQRLERKPDDVGVYGRQGMVGERTREEIGVHAVRGGDIV
                     GDHTVIFAGDGERLEIVHRAHSRQVFVSGVIKALRHVTYAQKGKISDMGDVLGLKK"
     gene            complement(952..1845)
                     /gene="dapA"
                     /locus_tag="PKK85_06470"
     CDS             complement(952..1845)
                     /gene="dapA"
                     /locus_tag="PKK85_06470"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNS25694.1"
                     /translation="MKLEGTTVAMITPFTTSDEVDEEGMRENINYLMDNGVEGLLAAG
                     TTGESATITHQEQRKMMDILVDEVKGRVKAIAGAGSNSSKEALGLVQYAEDAGADAAL
                     VITPYYNKPQPHGLYEHYRMLNDSTNIPIIVYNVPSRTGTDIDVDTIGQVAQLENVVA
                     IKEASPDMDKVSQEIQIIRKIGLEDKFQVISGNDNLTLPMISQGARGVISVVANVDPE
                     RMSRMVRMALEGDFAGAMDLHYELYDLMKVLFVESNPVPAKESLNIIGRPAGHVRMPL
                     APLRDENREKIVKVLKNLELL"
     gene            complement(1842..>2022)
                     /locus_tag="PKK85_06475"
     CDS             complement(1842..>2022)
                     /locus_tag="PKK85_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNS25695.1"
                     /translation="TVNSQDFIDTPGIITRITEPLRKNKINIVEISSSQTSVVIFVDW
                     NDGKRAYEMVRSVLE"
BASE COUNT          477 a          539 c          395 g          611 t
ORIGIN      
        1 tataccatgg atttattcat tttccctcat ttaaacaatt tattttattt tttcagcccc
       61 aatacatcac ccatgtcact tattttccct ttttgagcgt aagtaacatg gcgaagggct
      121 ttgataactc cactaacaaa tacttgccga ctgtgggccc ggtgcacaat ctccaggcgt
      181 tctccatcac ccgcgaagat tacggtatga tcaccaacga tatccccacc cctaacggca
      241 tggacgccta tttcttcacg ggtgcgctct cccaccatcc cctgtcttcc ataaacaccc
      301 acatcatcag gtttcctctc caagcgttga gctatgagtt cgaaggctgt tactgcagtt
      361 ccggagggag catccttttt atggtggtgg tgggcctcta tgatctccac atcatagtcc
      421 gggagcagac cagccagttc ctccaccatc ttaaagaaaa cgtttactcc aatggccatg
      481 ttggaggata tgactgccgc cacataattt tcctcaataa tccctttaat tatttccagt
      541 tgctcgtcac tgaatccagt ggtgcccacc accaggttca caccagcctg ggcagtggtt
      601 tttatggttt ccattgctgc accggctata gtaaagtcca ccagtacttg tggctttttt
      661 tccctcagta cttctggcag attttctgcc cccctgatct taacaccgat tttacccaca
      721 ccaatgatct ctcccacatc cttaccttcc aggggagtgt ggggtgcctc tattgctgct
      781 accagcctca tatcgtcctg ctggaggatg gattttatga ttttggatcc catcctaccc
      841 ccggcacctg ttaccgccac atcaatcatt ttctcatctc ctattcctca ctggatatat
      901 aaaaaaatat aaaaaataat taaaagaatt taagccccca atgtgggatg tttaaagcag
      961 ttccaggttc tttaagactt taacaatctt ttccctgttt tcatccctga ggggtgccag
     1021 gggcatgcgg acatgaccgg caggtctgcc tattatgttc aaggattctt tagcaggtac
     1081 cgggtttgat tctacaaaca ataccttcat caaatcatag agttcataat gtaaatccat
     1141 ggcccctgca aagtcaccct ccagtgccat tcgcaccata cgactcattc tctccggatc
     1201 tacattggcc accacgctta tcactccacg ggccccctga ctgatcatgg gcagggtgag
     1261 gttgtcatta ccagatatga cctggaattt atcctccaaa ccaattttcc ggatgatttg
     1321 aatttcctgg gaaaccttgt ccatgtcggg acttgcttcc ttgatggcca ccacgttttc
     1381 cagttgggct acttggccaa tggtatctac gtctatatca gtaccagttc gagaggggac
     1441 gttatacacg atgattggga tatttgtaga gtcgttaagc atccggtaat gttcataaag
     1501 tccgtgtggc tggggtttgt tatagtaagg ggttataacc agggctgcat cagcaccagc
     1561 gtcttcagca tactgaacca atcctagggc ttccttcgaa gagttactgc cagctccagc
     1621 aatggcttta acccgtcctt taacttcgtc caccaagatg tccatcattt tcctctgttc
     1681 ctggtgggtt atggtggcag attcaccggt agttccggca gccagtaagc cttcaactcc
     1741 attatccatc aaatagttga tgttctctcg cattccctct tcatccacct catcactggt
     1801 ggtgaagggg gttatcatgg ccactgtggt tccttctaat ttcattccaa gacgctcctt
     1861 accatttcat aagctcgctt accatcattc cagtccacaa aaattactac tgaagtctga
     1921 ctggatgata tttccacgat attgatctta ttctttcgta aaggttcggt tattctggtg
     1981 ataataccag gagtatcaat aaaatcctgg ctgttaacgg tg
//
LOCUS       DAOQVW010000288         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_71191_length_2022_cov_3.227250, whole genome shotgun sequence.
ACCESSION   DAOQVW010000288 DAOQVW010000000
VERSION     DAOQVW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71191_length_2022_cov_3.227250"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKK85_06480"
     CDS             complement(<1..332)
                     /locus_tag="PKK85_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25696.1"
                     /translation="MGTLAVFIMIILFFLLLAFVFSTALLTPLIGKKNLLFVVALGFV
                     VGIIGGAFFIAPVFNDIPGMVRGIHQSTSDSPEIIEANISTDVDVNKVISDIKTLEGV
                     QGVETVGIT"
     gene            complement(392..805)
                     /locus_tag="PKK85_06485"
     CDS             complement(392..805)
                     /locus_tag="PKK85_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNS25697.1"
                     /translation="MDLPEEWEKKALIVVGIVVVIIVAYAFNPFKSEPNVTTNNESVN
                     INPVTNMPFDQSSPNSTANTNETTNTTSELTMEQATNIAIQANPGYASGETSKETMVI
                     NGVTISVWRVTLTKSGSPSKTLYINAISGKIEKES"
     gene            complement(817..1227)
                     /locus_tag="PKK85_06490"
     CDS             complement(817..1227)
                     /locus_tag="PKK85_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25698.1"
                     /translation="MELDNRGLASAELLFVMLIALIIIGGMVSLINSRMANTQTGELG
                     VARIEGEKIATAIDTAYKNGKGYSIDISFPTGENFVDFTADVTTDGYVTVVYPGKTPI
                     KIKLTTTKIFTNYTLSSSNKYRIANNGTITITNI"
     gene            complement(1237..>2022)
                     /locus_tag="PKK85_06495"
     CDS             complement(1237..>2022)
                     /locus_tag="PKK85_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25699.1"
                     /translation="TTDAINNNTVATCMKLEAETGRQIYINNVPINSYSDKPFKPSQI
                     TIDQTDPYSFNVNIPQGFNLTVTQKGQNATIRTPPITTTVSIEGLEDPYVWVKSKDRL
                     SSIIYKYPYYTSQYNEYHLADQIYSSKLENLAACLNGTGNPSGITPRPYYFVDPDGLS
                     FFDRLEGRSTSTEPSNTRMSTFVVGDPLKEEPGYWVGMSCVDHEYFKATPGPNTITIT
                     NSVDGNTYVVRDYGAPGWVPVVFRLSDKYMTRLGLQSNYMFTP"
BASE COUNT          540 a          392 c          441 g          649 t
ORIGIN      
        1 gttataccaa ctgtttcaac tccctgaaca ccctccaagg tttttatatc agaaataacc
       61 ttgttaacat caacatcagt ggatatgttg gcctcaatta tttcgggact gtcactggtg
      121 gactgatgaa tgccacgaac catgcctggt atgtcgttga aaacaggtgc gatgaagaag
      181 gcaccaccga ttatgcccac tacaaagcct agggcaacca cgaataatag atttttttta
      241 cctataaggg gggttaaaag ggctgtggag aatacaaatg ccaggagcag gaaaaacagg
      301 atgatcatta tgaatacagc gagggttccc atgatctcat ctccacttca gtactagttc
      361 cattggactg gaggggttat atagtttaag attaagactc tttttctatt ttaccagaaa
      421 ttgcgttgat ataaagagtc ttagatgggg aaccactctt ggtcagggta actctccaca
      481 ccgagatagt aactccattg atgaccatgg tctccttgga tgtttcaccc gatgcatatc
      541 ctgggttggc ttgtatggct atgttggtgg cctgttccat tgtgagctcc gatgtggtat
      601 tggtggtttc attggtgttg gcagttgaat ttggagagga ttgatcgaaa ggcatgtttg
      661 ttactggatt aatgttaaca ctctcgttgt tggtggttac attgggttcg gatttaaagg
      721 ggttgaaggc gtaggccact ataattacaa ccacgatccc cactacaata agcgcctttt
      781 tttcccattc ttcaggtaga tccataaaaa aaccctttaa atattggtaa tggtgatagt
      841 accgttgttt gcgattctat atttattact actactaagg gtgtagttgg taaatatctt
      901 ggtagtggtt aacttgattt ttatgggagt tttaccaggg tagacaacgg tgacataacc
      961 atcagtggta acatcggctg tgaagtctac aaagttttcg ccggtaggga aactgatgtc
     1021 aatggaatat cctttaccat ttttataggc agtgtcaatg gctgtggcga tcttttcacc
     1081 ttcaattcta gccactccca gttctccggt ttgggtgttg gccattctcg agtttattaa
     1141 agaaaccatc ccgcctatga tgataagtgc gattagcatg acaaataata actcagcact
     1201 ggctaatcca cggttatcta gttccatctt tttcacctaa ggagtaaaca tgtagttact
     1261 ttgtaatcct aacctggtca tgtatttatc ggataatctg aaaactactg gaacccatcc
     1321 aggcgcacca tagtctctta ccacataagt attaccatca acactgtttg ttatggttat
     1381 ggtattgggt cctggtgttg ctttgaaata ttcatggtcc acacaggaca ttcccaccca
     1441 atatccaggc tcttctttta aaggatcccc tactacaaat gtagacattc gtgtattact
     1501 aggctctgtg gatgtggatc tgccctccag tctatcaaag aaactgagac catcgggatc
     1561 gacaaaataa taggggcgag gtgtaattcc acttggattc cctgttccgt tcagacaagc
     1621 agctaaattc tcaagtttac tgctataaat ctgatctgca aggtgatatt cgttgtattg
     1681 agatgtgtag taaggatatt tgtatataat actagataaa cggtctttgc ttttaaccca
     1741 cacgtagggg tcttccaacc cctcaattga tactgtagta gtaataggtg gagttctgat
     1801 ggtggcattc tgtcctttct gcgtaacagt taaattaaat ccctgaggta tattaacgtt
     1861 gaaactgtaa ggatcagtct gatcaattgt gatttgactt ggtttaaagg gcttatcaga
     1921 ataactatta atcggaacgt tgtttatgta tatctgtcga cctgtttcgg cctccagttt
     1981 catacaagtc gcgactgtgt tgttgtttat tgcatctgtt gt
//
LOCUS       DAOQVW010000289         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_71423_length_2017_cov_4.438328, whole genome shotgun sequence.
ACCESSION   DAOQVW010000289 DAOQVW010000000
VERSION     DAOQVW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71423_length_2017_cov_4.438328"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKK85_06500"
     CDS             complement(<1..272)
                     /locus_tag="PKK85_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNS25700.1"
                     /translation="MNKSFAILGIMLMVVAMTGCTSPPGTQTNSSTNPSGINWETDFN
                     AAFEKAQASNKMVFADFYADWCHYCKMMDGETYTDTNVQQKMAQSYV"
     gene            complement(366..1226)
                     /locus_tag="PKK85_06505"
     CDS             complement(366..1226)
                     /locus_tag="PKK85_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNS25701.1"
                     /translation="MKVHYECAPCFLRQAREALDLATDDEELKLKITSQIIDLISQRF
                     HPGAPANEIGTDMHRTIKKETDNPDPYHRERKISNEIALKFLPEVKKLLKEDSSLKSY
                     LKAAIAGNLLDFAALGLDVDMEALVLSAMHQELKIDHSSQLEEELKKAQNVLYLADNV
                     GEIVFDRLLLEKLEEYLVKVTVALKKKPILNDACLEDALEIGLDEVADLTTTGTDSVG
                     VIYKDVSLEFLELFYHSDLIIAKGLGNYEGLTEMDLKNKPVFCLFNAKCPPIAREVGV
                     EVGDSVVLKL"
     gene            complement(1283..>2017)
                     /locus_tag="PKK85_06510"
     CDS             complement(1283..>2017)
                     /locus_tag="PKK85_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNS25702.1"
                     /translation="TLAAGYILGNSWLGAVGFATALLAIIGTHESAHFIYARKHGVKA
                     TLPYFIPAPTIIGTFGAVINVKSPIPDRNALFDLGLSGPLAGLLVTVPVLMIGMSIST
                     VAAKSTSAMIFTLPLLMDIIAYFTAPAVSSGQMIFLHPVAFAGWVGVVVAMLNLMPAA
                     FLDGGHISRSIFSEKIHFIISFLAVAVTITLGWIPMAILMLLILFKSKSHPGAMDNVA
                     PITRGRKIMAILALVIFILCLSPAPS"
BASE COUNT          512 a          464 c          452 g          589 t
ORIGIN      
        1 acgtaacttt gggccatctt ctgttgcaca ttggtgtcgg tgtaggtttc accatccatc
       61 attttgcagt agtgacacca gtctgcgtaa aaatcagcaa acaccatttt attagaagcc
      121 tgggcctttt caaatgccgc gttaaaatct gtttcccagt taatgcctga tggatttgtg
      181 ctggaattgg tctgtgtgcc agggggggat gtgcagcctg tcatcgccac caccattaac
      241 atgattccca ggatagcaaa tgatttgttc atgatgcttg gttgtcactg ggagggaata
      301 tatttttttg ctctttgtcc ctgtaacccc tgggtaattt gccctctgaa tttaaatcca
      361 aatttttaca acttcaaaac cacactgtcc cctacttcaa cccctacttc ccgggctatc
      421 ggtggacatt tggcattaaa aaggcagaaa accggtttgt ttttcaggtc catttcagta
      481 aggccttcat agtttcccag tcccttggca attatcagat ccgagtggta gaagagttcc
      541 aggaattcaa gggacacatc tttgtagatc acacccaccg aatctgtacc agtggtggtt
      601 aaatctgcta cctcatctaa acctatctcc agtgcatcct ccagacaggc atcattcagg
      661 atgggctttt tcttgagggc caccgtgacc ttaaccaggt attcttccaa tttttcaagg
      721 agcaacctgt caaaaactat ttctcccaca ttatctgcta agtataaaac attttgggct
      781 tttttaagtt cctcttccag ttgggaggaa tgatctatct tcaattcctg gtgcatggct
      841 gacagtacca gggcctccat atctacatcc aaacccagtg ccgcaaaatc cagtaagttc
      901 ccggcaatag cggctttcaa ataacttttc agactggaat cttcttttaa taattttttt
      961 acctctggta agaatttgag ggcgatttca ttggatattt tccgctcccg gtgataggga
     1021 tctggattat cagtttcctt cttgatggtg cggtgcatgt cggtgccgat ttcattggcc
     1081 ggtgccccag ggtggaatct ctggctgatt agatcgatga tttgggatgt gatcttcaac
     1141 tttaattctt catcatcggt agccagatcc agggcttccc gggcctgtct tagaaaacag
     1201 ggggcgcatt catagtgtac tttcataaga tcttcccatt ctttttttta atggctgtta
     1261 acttagagtt cttcaaatcc atttatgaag gtgccggtga taggcacagt ataaatatga
     1321 ccagggccag tatggccatt atttttcttc cacgggtgat gggagccaca ttgtccatgg
     1381 cacctgggtg gcttttgctc ttgaagagta taagtaacat caggatggcc atgggtatcc
     1441 atcctaatgt gatggttaca gccaccgcca agaaggatat gatgaagtgt atcttttcac
     1501 tgaagatgga cctggatata tgccctccat ccaggaaagc cgctggcatc agatttagca
     1561 tagccaccac cactcccacc caaccagcga aggccacggg atgtaaaaat atcatctgac
     1621 ccgaagagac cgcaggggct gtgaagtaag ctataatatc cattaatagg ggtagtgtga
     1681 atatcatggc actggttgat ttggcagcta ccgtggagat gctcatacca atcatcaaaa
     1741 ctggcacagt taccagcaga ccagccagtg gaccgcttaa acccagatca aaaagggcgt
     1801 tacggtctgg aatgggggat ttgacattta taaccgcccc aaatgtgcct attatagtgg
     1861 gtgctggtat aaaatagggt aaagtagctt taacaccatg tttacgggca taaataaagt
     1921 gagcagattc gtgtgtgcca attatggcta gtaatgctgt tgcaaatcct actgccccca
     1981 gccaactgtt ccccaggatg taacccgcgg ccagggt
//
LOCUS       DAOQVW010000290         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_71647_length_2014_cov_1.872384, whole genome shotgun sequence.
ACCESSION   DAOQVW010000290 DAOQVW010000000
VERSION     DAOQVW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71647_length_2014_cov_1.872384"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(417..>2014)
                     /locus_tag="PKK85_06515"
     CDS             complement(417..>2014)
                     /locus_tag="PKK85_06515"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNS25703.1"
                     /translation="NARKIPAGVKKRLLSNKNSPEAFGERMQFRIQELLGNPDSYTPS
                     YKKRYKMLLEYCDSLTPMQAYAMNQLLGMDSSRGYQDIPDESNIEFPRDFAPQLGFQV
                     GWHFIVGNCRDTRRREYGILVSFYRYSLLPPWIAQSFGLSDWDNQIFEMQLAVARAGE
                     EHLQTRPFAIAGTTGLLKCSLEPFHYEAGNNRMVSEKEDELFPLRVQAWGVNQGGEED
                     VEMEVDLNLTSNKEFLLQGNKGCLPCCCSIGTLYYSATNLSLEPGSLLILDGEEIMLN
                     QGQFWFDHQWGNALEPMGNSRCEVVRAAGTLSKPSQSRGWDWFMAQFSGEREMTMYAP
                     HTDKNLDFYERTEEEPPGPMEVAVKGQYIDPENKVTDMKGILTIDKWVKSVKSSNPEL
                     YLITNTWYPDHWKFRFEDMVPEDIRHFTLTPIVETGQTGYNAGGSQYSEGGVNIRDPD
                     GKFLGKGFAESVYYADSHPNVLHLAGMPDTPEMRKLLKPQEPSPLLKLKGFLYMAWPP
                     HQKKIKNILEKCLEQGLPADYLG"
BASE COUNT          445 a          509 c          476 g          584 t
ORIGIN      
        1 tagtcctgtg ggtttctgga tccgaagtga tcgaaaagta ggaatggatc gagatagtcc
       61 agtctctggg tggctatgct cctgcgcagt agaacaccag caccctccat cacatagata
      121 ggttcaataa tatcagttac ttcccttact gttgccatat aaataacccc ctgttaaatt
      181 tacttactag atgaagttta ctcgcatttg gaaatattcc ccttacactc aactggtata
      241 ttattatatg gtttgctctt tatctaattt ttcaaaccct ttttctaatt tatggggaag
      301 ttaggggtta taagagtttc aggacttacc ctaaataatc aatggtgttt gtgacttatc
      361 ccaagtaatc aacaggcatt aggaccctat cccagataat caaccggttc tcggtcttat
      421 cctagataat cagctggtaa tccttgttcc aggcattttt ccaggatatt tttaatcttc
      481 ttctggtgtg gaggccaggc catgtataag aagcccttca actttagtag gggtgatggt
      541 tcctgtggtt ttaagagttt cctcatctcc ggcgtatctg gcatccctgc caggtggagg
      601 acgttggggt gtgagtcggc gtagtagact gactctgcaa atcccttgcc caggaattta
      661 ccatctggat ctcggatatt cactccaccc tcagagtact gggatccacc ggcgttgtag
      721 ccggtctgac ctgtctccac gataggtgtc aatgtaaagt gccggatatc ttcaggaacc
      781 atatcctcga acctgaactt ccagtggtcc gggtaccagg tgttggttat gaggtaaagt
      841 tcagggttgg atgacttcac actcttaacc catttatcga tggttaaaat acctttcata
      901 tcggttacct tgttttccgg gtcgatgtac tgtcccttca ccgctacttc catgggtcct
      961 ggtggttcct cctctgtccg ctcgtagaag tccaggtttt tatcggtgtg gggtgcgtac
     1021 atggtcatct ccctttcacc actaaactgg gccatgaacc agtcccatcc cctggactgg
     1081 gatggtttgc tcagggtgcc tgctgcccgg accacctcac accggctgtt gcccatgggc
     1141 tccagggcgt tgccccactg gtggtcgaac cagaactggc cctggttaag catgatctcc
     1201 tcaccgtcaa gtattaaaag actgcccggc tccagggaca ggttggtggc agagtagtag
     1261 agggtaccta tactacaaca gcaggggagg catcccttat tcccttgaag aaggaattcc
     1321 ttgttggatg ttaaattcag gtccacctcc atctccacat cctcttcacc gccctggtta
     1381 actccccagg cctgcaccct gagtgggaat agttcgtcct ccttttctga gaccatcctg
     1441 ttatttcccg cctcgtaatg gaagggttca agggagcatt ttaaaagccc cgtggtcccg
     1501 gctatggcga agggcctggt ctgcaggtgt tcttcccctg cacgggctac cgccagttgc
     1561 atctcaaata tctggttatc ccagtcagac aggccaaagc tttgggctat ccatgggggt
     1621 aagagtgaat agcggtagaa cgataccagt atcccatatt cccgtcttct ggtgtcgcgg
     1681 cagtttccca ctatgaagtg ccagcccacc tgaaaaccta gttgaggggc gaagtcccgt
     1741 ggaaactcaa tattagattc atccgggatg tcctggtagc cacggctgct gtccattccc
     1801 aggagctggt tcattgcata ggcctgcatt ggtgtgagcg aatcacagta ctccaggagc
     1861 atcttgtacc tcttcttata gctgggggtg tatgaatcag ggttacccag taattcctgg
     1921 atacggaact gcatcctctc cccaaaggct tctggactgt ttttattact taataaacgt
     1981 ttttttacac ccgcaggtat tttcctggca ttga
//
LOCUS       DAOQVW010000291         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_72166_length_2005_cov_3.026667, whole genome shotgun sequence.
ACCESSION   DAOQVW010000291 DAOQVW010000000
VERSION     DAOQVW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72166_length_2005_cov_3.026667"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..689)
                     /locus_tag="PKK85_06520"
     CDS             complement(165..689)
                     /locus_tag="PKK85_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HNS25704.1"
                     /translation="MKSRSMEEHRRNSPLSVKVGVITLSDSKYQDFLQGKTDTDLSGQ
                     LLKDSLSEVHQVVDYKIIPDDPPELISTLEAMKGKGAEVIITTGGTGIGSRDKTIETL
                     KPLFGKKLDGFGEIFRYESYKELGAGAILSRAMAGVYDSTLIFALPGSPNAVKLGLKI
                     IQPELGHLVRHIKE"
     gene            1082..1621
                     /locus_tag="PKK85_06525"
     CDS             1082..1621
                     /locus_tag="PKK85_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease VapC"
                     /protein_id="HNS25705.1"
                     /translation="MASFSHSNNKKCKVYVLDTSAIIGRFLSPDTTNITTNGVIMEIK
                     DFNSRIFTENAREDGKLLVREPAPWAFKKVKRMMEESGDVLRLSGVDIGVLALAVTLT
                     ESYQPQLVTDDYSMQNLSKILKIPYRGVLTPGIEDVYHWILICQGCKKRYQSHYNGSD
                     CEICGSKLIKRRIKVSKRI"
     gene            1703..>2005
                     /locus_tag="PKK85_06530"
     CDS             1703..>2005
                     /locus_tag="PKK85_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HNS25706.1"
                     /translation="MKIGVVVHGPEIVDSGYALRLLEFMEDYGEVKARLGGTMGRTAV
                     IDSNLESRIDISQKLLPSQSINLFCQEGRDIIVLINYGKSSVTGQAFGCKVYSHCHP"
BASE COUNT          562 a          420 c          453 g          570 t
ORIGIN      
        1 ggccttggtg gctgtggatc gtgaagaagg ggcccgggag aatcttcaga aaaaaggtat
       61 taaactggag ccactggtta ctaaaagtga cttccagggt gcgtaactag accttgaaga
      121 tgcccctact aatcccggtc cctattaggg ccattcatgg ttaattattc tttaatatga
      181 cgaactaggt gtcccagttc gggctggatg atcttcaaac ccagcttcac ggcgttgggt
      241 gaaccaggta gtgcgaagat cagggtagaa tcgtaaactc ctgccatggc ccggctgagg
      301 atggcgccgg ctcccaactc cttgtaagat tcgtaacgga atatttcacc aaaaccgtcc
      361 agttttttac caaagagtgg tttaagggtc tcaatggtct tgtctctgga accaattcca
      421 gtaccaccag tggtgataat gacctctgct cccttccctt tcatggcctc cagggtggag
      481 atgagctctg gtgggtcatc gggtatgatc ttgtaatcca ccacttggtg cacttcactt
      541 aaggagtcct ttaacagctg ccccgaaagg tcggtgtcgg ttttaccctg gaggaaatcc
      601 tgatatttgg agtcgctgag ggttataact ccaactttaa cactaagagg gctgttcctt
      661 cgatgttctt ccatgcttct gcttttcaat caaatcacat cctaagttcc cagttgtgaa
      721 taaaaaaaat gtgtccatgg ggcaaataaa gtcctaggtt cacactggag aatactcttc
      781 caatcccctt tatataatat tttagctgaa ccggtacaaa atatagaatt aacccatata
      841 tatgtggagg gagagttttt aggtccaaat cggaggcggt tttttcgtgg cgaaaaaatc
      901 ttgaggagtc taaatagtat ctctccctcg gtgggaaagt ttattcttta taacataaaa
      961 acctttctca taagcccctc taccaattac aaaaacgaat ttaaaagttt taaactcata
     1021 aattcccaat aaaaaatggt ttggaggata aagaggttta aaaaatttca aagggtgttt
     1081 tatggccagc ttttctcatt ctaataataa aaaatgcaaa gtatacgtcc tggacacatc
     1141 ggccattatt gggagatttt tatcacccga tacgactaat ataaccacaa atggggttat
     1201 catggaaatc aaggacttca attcccgtat tttcaccgag aatgcccggg aagatggcaa
     1261 gctgctggtg agggaaccag ctccctgggc ttttaaaaag gttaagagga tgatggagga
     1321 atctggcgat gtactgaggc tttcaggggt tgatatagga gtcctggcat tggcagttac
     1381 tttaacagag tcctatcaac cccaactggt taccgatgac tactccatgc agaatctgtc
     1441 taaaatcctc aaaataccct accgtggcgt gttaacccct ggtattgagg atgtttatca
     1501 ctggattctg atctgccagg gatgtaagaa gcggtaccaa tcccactata atggttcaga
     1561 ttgtgaaatt tgtggttcta aactaatcaa acgacgaata aaagtatcta aacgaatctg
     1621 atcagtattg tggtctgtac atccatttac tactttttta ttttagttaa ttataagagg
     1681 ctacagatcc taaggtgaaa taatgaagat tggtgtggtg gtccacggac ccgaaattgt
     1741 ggattctgga tatgcccttc gcttactaga atttatggag gactatggag aggtgaaagc
     1801 ccgcctgggc ggtaccatgg gaagaaccgc agtaatcgat tctaatctcg aatcccggat
     1861 tgatatcagt caaaagcttc tgcccagtca atccatcaat ctcttctgcc aggaaggtcg
     1921 ggatattatt gttctcatca actatgggaa gtccagtgtc actggccaag cctttggttg
     1981 taaggtttac agccactgcc accct
//
LOCUS       DAOQVW010000292         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_72226_length_2004_cov_3.060031, whole genome shotgun sequence.
ACCESSION   DAOQVW010000292 DAOQVW010000000
VERSION     DAOQVW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72226_length_2004_cov_3.060031"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..530
                     /locus_tag="PKK85_06535"
     CDS             345..530
                     /locus_tag="PKK85_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNS25707.1"
                     /translation="MPVITIDAPPLSREQKSQLVKSFAETASEVMNLPVSAMVFIINE
                     VEPENVGVGDILLCDRE"
     gene            604..>2004
                     /locus_tag="PKK85_06540"
     CDS             604..>2004
                     /locus_tag="PKK85_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNS25708.1"
                     /translation="MVPDKKKFTNHLKDEKSPYLKQHAHNPVDWYPWGEEAFQKALYE
                     DKPVFLSIGYSTCHWCHVMARESFQDEEIGQILNQYFVSVKVDREERPDIDSIYMTVC
                     QMMTGSGGWPLTIILTPNQEPFYAGTYFPRMSVGRGVGLKDLLLNVKDMWSDKKQELL
                     DSAQDIRSALEDLNRTAPGDLLDLDAVDGAYQALKDSFDQENGGFGGFQKFPTPHHLL
                     FLLRYWRATGKEEALVMVESTLEHMIRGGLWDHVGYGFHRYSVDPHWLVPHYEKMLYD
                     QALITLALTECYQATHKTLYRETVEKTLEYVFRDMKSLEGGFYSAEDADSEKVEGKFY
                     LWTHQELSQILDPEELELVEKVYRVQEKGNYREESTGQYTGHNILHLEEPLEESAYRL
                     GLGHDELKNKLEMIRVKLFKARMGRVRPHKDEKVLTDWNGLMLVALARASVVLDNQDY
                     LDTALNSLDFIKSKLYR"
BASE COUNT          507 a          474 c          540 g          483 t
ORIGIN      
        1 tgcaggtgca tctggctcct ttctcatttt caaaggacct gtggagggat aggacaggta
       61 gagttggtcg aaattccatc gcaacctatg ggggtccctt tcctccaggt ccacctggct
      121 ttttattaac tccagtaatt tccgggtttc ctctttcatc tcccttcccc taatcattat
      181 ctttgctttg ggatacgtct atgcactggt atctttgatc tgcaagtatc ttttatgtaa
      241 ctggggggct atcaactata gcaaaaaaat atcatggggc ctgatccagg agaaaattta
      301 atgtcccccc tggtagagat gtatgaacag gaggtgagat ttggatgccc gtaataacca
      361 ttgatgcacc gccacttagc agggagcaga agtcccaact ggttaaatcc ttcgcggaaa
      421 cagccagtga agttatgaat ctcccggtta gtgccatggt gtttatcatc aacgaagtgg
      481 aaccggaaaa tgtgggggtg ggtgatatcc tgctctgtga ccgggaataa attttttttt
      541 attcaagttt caaatttttt ttttatcaaa gataattata cagaagatgg gggtgaggga
      601 gacatggtcc ctgataagaa aaagtttacc aaccatttaa aggatgagaa aagtccctac
      661 ctgaagcaac atgcccataa cccggtggac tggtacccct ggggtgagga agccttccaa
      721 aaggctttat atgaggataa gcctgttttt ctttcgattg gctattcaac ttgtcactgg
      781 tgtcatgtca tggcccggga gtccttccag gatgaggaaa tagggcagat cctgaaccag
      841 tacttcgtgt cggttaaggt ggaccgtgag gagcgccctg atattgacag catctacatg
      901 accgtctgtc agatgatgac tggatccggg gggtggccct tgaccatcat actcaccccc
      961 aaccaggaac ccttctatgc cgggacctac tttccccgga tgagtgttgg ccggggagtg
     1021 ggacttaagg acctgctcct gaatgttaag gacatgtgga gtgataaaaa gcaggaactc
     1081 ctggattcag cccaggatat tagaagtgcc ctggaggatt tgaaccgcac agcaccagga
     1141 gacctcctgg atcttgatgc agtggatggg gcctaccagg cccttaagga cagttttgac
     1201 caggaaaatg ggggctttgg cggtttccag aagttcccca ccccccatca tctgctcttc
     1261 cttttgaggt actggagggc cactggaaag gaggaggccc tggtgatggt ggagtccaca
     1321 ctagagcaca tgatacgtgg cgggctctgg gaccatgtag gctatggctt ccaccgttac
     1381 tctgtggacc cccactggct ggtgccccac tacgagaaga tgctctacga ccaggccctc
     1441 atcaccctgg cactcaccga atgttaccag gccacccata aaaccctgta cagggagacc
     1501 gtggagaaaa ccctggaata cgtattccgg gatatgaaat ccctggaggg tggcttctac
     1561 tctgctgagg atgcggatag tgaaaaagta gaggggaaat tctacctgtg gacccaccag
     1621 gaactgtcac agatcctgga cccggaagaa ctggaactgg ttgaaaaggt gtacagggta
     1681 caggagaagg ggaactaccg tgaagagtcc actggccagt acaccggcca taacatcctc
     1741 cacctggagg aaccacttga agaaagcgcc tatcgtctgg ggctgggcca tgatgaattg
     1801 aagaacaaat tggagatgat ccgggttaaa ctcttcaagg cccggatggg gagggtgcgc
     1861 ccccacaagg atgagaaggt actcactgac tggaatggat tgatgctggt ggccctggcc
     1921 cgggccagtg tggtgctaga taaccaggac tacctggaca ctgccctgaa ctctctggac
     1981 ttcatcaaat ccaaactcta cagg
//
LOCUS       DAOQVW010000293         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_72275_length_2003_cov_3.901437, whole genome shotgun sequence.
ACCESSION   DAOQVW010000293 DAOQVW010000000
VERSION     DAOQVW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72275_length_2003_cov_3.901437"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(554..1219)
                     /locus_tag="PKK85_06545"
     CDS             complement(554..1219)
                     /locus_tag="PKK85_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS25709.1"
                     /translation="MSNNKTNMPEKGAAVQKDMETYAINPYIPGGLVEPDTLRKIADV
                     AEKYDAKFIKLSSEHRITIFGIPYQDVDEIWNDLEMEPGGLSGKIVRPVKFCIGKSSC
                     KMAKQNTMEIGLEIDQRFRGTKTPDKMKIAVSGCENSCAEPGVRDIGLIGTNKGWKVL
                     VGGNAGIKPRLGDLIANDLSSEEVLALMDKIINYYIENEESVRLGRFIDNMGLDEFKK
                     EIL"
     gene            1395..>2003
                     /locus_tag="PKK85_06550"
     CDS             1395..>2003
                     /locus_tag="PKK85_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNS25710.1"
                     /translation="MNEELVMVRYGEIGVKSPAVRRRFEKRLVNNIKSALKCQSTVEQ
                     GRIFLKVEDTAETLELLARTFGVVSFSPVVYTDTDFDAIKEELTIYTNELVEEGVFSG
                     EDSFAIRCRRVGQHDFTSQELAAFAGSVVHGVTQSPVDLSHPDFEIFLEVRDDRTYIF
                     HQKISGPGGLPIGTQGRLIALLSTGIDSPVACYLMMKRGCSVT"
BASE COUNT          521 a          479 c          411 g          592 t
ORIGIN      
        1 ctagctggtt ttatcaaaac caccaccggg tcctacctgg gggtttttcc ctacgtcctg
       61 gcactggccc tagtaggcat ggccctagct ggggcccttc taagaccacc ccgggttgag
      121 gtagaacaaa aaagctaaaa ttaagctacc acctcctcca tcccgggact cgctagcccg
      181 ggattttcta ttttttttta aattcctgat aaaaaaaatg accaacactt tttcatgacc
      241 tttcatacac ccatcaggga ggagggtggg ctagtaagga tgttttgtgg ccatcaaagt
      301 gggtgttcac ctaacatcga agtgcaattc atttctgcct ccatggagaa cgttattgaa
      361 ccctggatta tggtcagttc cctgcccatc catggcacca caaaatcccc tggcacgtat
      421 ctacaaaaaa aagggctgac catcctggat gagcatactc ctgtgccatt gcacgctcca
      481 tccacgatgg ttttttttat attcacttgg ctggatacat tttttggcga atttctacac
      541 tacccctaag tactcataaa atttcttttt tgaattcatc caatcccatg ttgtctataa
      601 atctacctaa acgcacactt tcttcgtttt ctatgtagta gttaataatt ttgtccatca
      661 aagccagaac ttcttcactt gataggtcgt tggcaatcaa atcaccaagt cggggtttaa
      721 tacccgcgtt accacccacc agaactttcc agcctttatt ggttccgata agtccaatat
      781 ccctgactcc aggttctgca catgaatttt cacaaccgga aacagcgatt ttcatcttgt
      841 caggggtttt tgtacctcta aaccgttggt caatttccaa tcctatttcc attgtattct
      901 gcttggccat cttacaggaa gattttccaa tacaaaattt gacgggtctg actattttac
      961 ctgacaaacc acccggctcc atctccagat cattccagat ctcatccaca tcttggtagg
     1021 ggattccaaa tatggtaatt ctatgttctg atgataattt aatgaattta gcatcatatt
     1081 tttctgctac gtcagcaatt ttccttaaag tatcgggttc aacaaggcct cctgggatat
     1141 aaggattaat tgcataggtt tccatgtcct tctggactgc tgcgcctttt tctggcatgt
     1201 tggtcttatt atttgacatt tttacacctt tatttcctca agtctatgga taaatctata
     1261 atgattatta ctttatataa atttattttc gaaaatgata gccttaaatc catgaaccat
     1321 caaaccatat gatacgaaga ttacacagcc ccctaaaatg gttaaaaatt gttaatctaa
     1381 ttagtttgat tcctatgaat gaagaactgg tcatggtccg ctacggggag ataggtgtta
     1441 aaagtccagc ggtgaggagg aggttcgaga aaagactggt taacaacatc aaatcagccc
     1501 tgaagtgcca gtccactgtg gagcagggac gaatcttcct caaagtggag gatactgcag
     1561 aaaccctaga attgctggcc agaacctttg gtgtagtgtc cttcagcccg gtggtgtata
     1621 ctgacaccga ttttgatgcc ataaaagaag agttaaccat ctacactaat gaactggttg
     1681 aggagggagt gttctcggga gaggattcct tcgccatccg ctgccgccgg gtgggtcagc
     1741 acgacttcac cagccaggaa ctggcggctt tcgccggatc agtggtgcat ggagttaccc
     1801 agtcccctgt ggatctttcc catcctgatt ttgaaatatt tctggaggtg agggatgaca
     1861 ggacctacat cttccaccag aaaatctccg gcccgggtgg actgcccatc ggcacccagg
     1921 gcaggttaat agctctccta tccacgggga tagactctcc agtggcctgc taccttatga
     1981 tgaagcgggg ttgcagtgta aca
//
LOCUS       DAOQVW010000294         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_72304_length_2003_cov_2.540041, whole genome shotgun sequence.
ACCESSION   DAOQVW010000294 DAOQVW010000000
VERSION     DAOQVW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72304_length_2003_cov_2.540041"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKK85_06555"
     CDS             complement(<1..721)
                     /locus_tag="PKK85_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNS25711.1"
                     /translation="MKLKHGLGLVLILIIATAVYSTIITEKQYERIDVAGSTSVQPVA
                     EALAQEYMKAHPRVKITVQGGGSGLGIRSVSQDIVAIGTSSMELNDNEGGKLKEYVIG
                     KEGIVVAVNLKNPVDVISKDDLRAIFTGNISNWKELGGPDAPINVVVREDGSGTRKTF
                     DDLVLKKTDKVKSDAIVQISTESIKLVVEQDPNAIGYISLAHMSPNVKAVKVDGVYPS
                     METILGGTYELHRPFLFLTKGE"
     gene            complement(1224..>2003)
                     /locus_tag="PKK85_06560"
     CDS             complement(1224..>2003)
                     /locus_tag="PKK85_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HNS25712.1"
                     /translation="VKKGVVRRAYILDFKKFDEFSEIFHEEATSREHAGSYLIKEMLS
                     DMAQHVCQQLEMSFEAFLNHDTALAEEALKLDYITNNMLEKVRSSVETAVSMNPYSEF
                     SKTMIFGEIAFDLERIGDHSCHIANFALNESYEIDAEMMGIVKSMYDTACRMMTHSMN
                     AFTNEKLELKDKVMDYEEELHNLQGEAMGRIAHQMAEDDLMDQERSTYYIYLSRVVKA
                     FERIGDISIEIMDTAGEFYRNIPRTTTPERFQNRYKKSSKF"
BASE COUNT          569 a          434 c          360 g          640 t
ORIGIN      
        1 tctctccttt ggttaaaaag aggaatggcc tatgtaattc ataggtgcca ccaagaatgg
       61 tttccatgga tggatacact ccatccactt tcacggcctt aacgttgggg ctcatatggg
      121 ccagtgaaat atagccaatg gcattggggt cctgttccac aaccagttta atggactccg
      181 tggaaatctg gacaatggca tcacttttaa ccttgtcagt ttttttcaat accagatcat
      241 cgaatgtttt cctggttcca gagccatctt ctcgaactac cacatttatg ggagcatccg
      301 gaccccctaa ttccttccag ttacttatat ttccagtgaa aatagccctc aaatcatctt
      361 tgctaatgac gtccacagga ttttttaggt tgacagccac cacaatccct tcctttccaa
      421 taacatattc cttcagcttt ccaccctcgt tatcatttaa ttccatggaa ctagttccaa
      481 tggcaactat atcttgtgaa acactgcgaa tacccagtcc agatcctccc ccctggacag
      541 tgatcttaac tctgggatgg gctttcatat actcctgggc cagtgcttca gccacgggct
      601 gtacagaagt agaaccagcc acatcaattc gttcgtactg cttctcggta atgatggtgc
      661 tgtaaacggc ggtggcaatt attaagatta gtactaatcc caggccatgt ttcaatttca
      721 ttgcgaaatc accataaaaa atactgatta gagaataatt tccattagca gtatttataa
      781 ttatactgtg attagactcc tgaagttttt aagaaactta tttcactact gaagattaat
      841 caagaccaaa aaatatagga tgagaaaaaa tagggttagt ggatagattc caccctattt
      901 acacttttaa attttttttt gtctttactc ctgggggaga gttaatcctt gaatgaacaa
      961 tgatcctcat tgtatactgt ggtccaaatc accttcaatt ccagagtaac cagacattct
     1021 atttaagggt tactgattgt gtttaatata tcactatttg tgaaataaag tagggggtgg
     1081 ggagtcttca ttctaatcac tttacctctt gtgaaaatgg atggtatgat ctagaacacc
     1141 aaaaaaaacg catttttcta aattatgctt atatacactt tcaatccatt gaatagggag
     1201 atttagtatc ctctatccat ccattaaaat tttgaagact ttttatacct attctgaaac
     1261 ctttccggag tggtggttcg ggggatgttc cggtagaact ctccagcagt gtccatgatc
     1321 tcgatggaga tatcaccgat cctttcaaag gccttgacca cccgggagag atatatgtaa
     1381 taggtggatc tttcctggtc catcagatca tcttcagcca tttgatgggc tattctcccc
     1441 atggcttcac cttgaagatt gtgcaactct tcctcatagt ccatgacctt gtctttgagt
     1501 tccagttttt cgttggtgaa tgcattcatg gaatgagtca tcattctaca ggcagtatcg
     1561 tacatggatt taactatacc catcatttct gcatcaattt cataagactc atttaaagca
     1621 aaattagcta tatgacagga atgatcacca atacgctcca gatcaaaggc aatttctcca
     1681 aatatcatgg ttttactgaa ttcagaataa ggattcatgg ataccgcagt ttccactgaa
     1741 gaacgaacct tttccaacat attattagtg atataatcta atttcagggc ttcttcagcc
     1801 agtgcagtat catggttaag gaatgcctca aaagacattt ctagctgctg acaaacatgt
     1861 tgggccatgt cactgagcat ttccttgatc aggtaggacc cggcatgttc acgggaagtt
     1921 gcttcttcat gaaatatctc agaaaactcg tcaaatttct tgaaatctaa tatatatgct
     1981 cttcgaacta ctcctttttt tac
//
LOCUS       DAOQVW010000295         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_72463_length_1999_cov_5.183642, whole genome shotgun sequence.
ACCESSION   DAOQVW010000295 DAOQVW010000000
VERSION     DAOQVW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72463_length_1999_cov_5.183642"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..467
                     /locus_tag="PKK85_06565"
     CDS             222..467
                     /locus_tag="PKK85_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25713.1"
                     /translation="MFSKNLFASDLESAIGRDCTFHLKPGHPIGECITARVTSARMSL
                     GDNLYLFVEYEGQKFLLKSRDFTTFTIPSQARVEESL"
     gene            464..625
                     /locus_tag="PKK85_06570"
     CDS             464..625
                     /locus_tag="PKK85_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25714.1"
                     /translation="MTTPPLDRCRRCLIPLPPNYDVVIMDADEGVTVYRKTCPLCGTH
                     HDTTLEAPG"
     gene            737..1297
                     /locus_tag="PKK85_06575"
     CDS             737..1297
                     /locus_tag="PKK85_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25715.1"
                     /translation="MDKREYYGHPVRHDLIKSIFERNQDRFYKILSDKLKFLKHYRIR
                     KIKTEFPVYHRMARKIKMYSLKTTVDPSGYIDLMLILDGFGEKEGLNRALCFEIKDGA
                     FDFEKDILKYKNMENVFDNLPVIPLDSSEDPLIVLAWDEEIQNATKNKPNQIESNFHY
                     KVRFVPLDLFFEEIQQTYKEVGEGIV"
     gene            1294..>1999
                     /locus_tag="PKK85_06580"
     CDS             1294..>1999
                     /locus_tag="PKK85_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25716.1"
                     /translation="MTLSLKGIKQHYHHPLIKETIIRCSKNEHSTRAGNYDFTGWYKY
                     NQDGRQLYQLETDYDEIINKVQRSLYWTLNLFENRTFQEVAEPDEKIGTGKDTTAYTL
                     GIDIDIKEGYDITNSHARLSLEECVKFYYDELSQYLTNNIYAAFSGGGAYLYIHHELF
                     QLPLDNGQTPEERRDSWAVLRAMFQLLMEDISEKFFQQNPDAAQYVKVDYLNNDKRLF
                     KTIYSIHKEHDYAVIPL"
BASE COUNT          710 a          403 c          410 g          476 t
ORIGIN      
        1 cggaaccaag tcagcctttg gtagattaca gcagttgatg aaggatgagt tcggggtgaa
       61 gaaccggaag gacatgatcc ggcagttgaa gggtgctaag gttaagatta aaaccgaaac
      121 caccacttac aatggccagg agtacaggaa gaacatgata caaggattcc gataaataac
      181 ggggtccatt actttatttt tttttggagg cgaaaaagaa gatgtttagt aaaaacttat
      241 tcgcaagtga cctggaatca gccataggcc gtgattgtac cttccaccta aaaccaggcc
      301 accctatcgg agaatgcatc accgccaggg tgaccagtgc taggatgagc ctcggagaca
      361 acttgtacct gttcgtggag tatgaaggcc aaaagttcct actgaagagt cgggacttca
      421 ccaccttcac catacccagc caggcaaggg tggaggaaag cctatgacca caccccctct
      481 ggataggtgc cggcgatgcc tcataccact accccccaac tacgacgtag taatcatgga
      541 cgcagatgaa ggggttacgg tgtaccggaa aacctgcccc ctctgtggaa cacatcatga
      601 caccaccctg gaggcccctg gatgaccaac accctcagca gcctattaga tcccatcata
      661 ttctttttga tgatcctagt ggtagtcgta accgtgggca tcctagcagt agaaatcaag
      721 tacctggagt gattaaatgg ataaaagaga atattatggt catccagttc gtcacgactt
      781 gattaaaagc atatttgaaa gaaaccaaga ccgattctac aaaatattaa gtgacaaact
      841 caaattccta aaacactatc ggatcagaaa aatcaaaaca gaattcccag tataccacag
      901 gatggcaaga aaaatcaaaa tgtacagcct aaaaaccact gttgacccta gtggttacat
      961 agacctgatg ctaatcttag atggatttgg agaaaaagaa ggactgaata gggcattatg
     1021 ttttgaaata aaagatggag catttgactt tgaaaaagac atcctaaaat ataaaaatat
     1081 ggaaaatgtc tttgataacc tgccagttat cccattagat agtagtgaag acccattaat
     1141 tgttttagca tgggatgaag agatacaaaa cgcaacaaag aataaaccaa atcaaataga
     1201 atctaacttc cattataaag ttcgatttgt tcctttagat ctattctttg aagaaataca
     1261 gcaaacctac aaagaagttg gtgaggggat agtgtgacac tctccttgaa aggaataaaa
     1321 caacactacc accatcccct aataaaagaa accataatca gatgcagtaa aaacgaacac
     1381 agtactaggg caggaaacta tgacttcacc ggatggtaca aatacaacca ggatggccgg
     1441 caattatacc aacttgaaac cgattatgat gaaataatta ataaggtaca acgcagttta
     1501 tactggacac ttaacctatt tgagaataga accttccaag aagtagcaga accagatgag
     1561 aaaataggca ctggtaaaga caccactgca tatacgttag ggatagatat tgatattaaa
     1621 gaaggatacg acatcacaaa cagccatgcc aggttaagct tagaagaatg tgttaaattc
     1681 tattatgatg aactaagcca gtatctaact aataatatat atgctgcttt cagtggaggt
     1741 ggggcctatc tatacatcca tcatgaatta ttccaactac cccttgataa tgggcagaca
     1801 ccagaagaac gtagggacag ttgggcagtc cttcgagcaa tgttccaatt actcatggag
     1861 gatattagtg aaaaattttt ccaacaaaac cctgatgctg cccagtatgt taaggtagat
     1921 tacctgaata atgataaaag actattcaaa acgatataca gtattcataa agaacatgat
     1981 tatgctgtta tcccattaa
//
LOCUS       DAOQVW010000296         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_73057_length_1989_cov_3.350052, whole genome shotgun sequence.
ACCESSION   DAOQVW010000296 DAOQVW010000000
VERSION     DAOQVW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73057_length_1989_cov_3.350052"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /gene="deoC"
                     /locus_tag="PKK85_06585"
     CDS             complement(<1..391)
                     /gene="deoC"
                     /locus_tag="PKK85_06585"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNS25717.1"
                     /translation="MMDAQELAQKIDHTNVGRDATWNDIEKLCDEAELYGFRNVCVTP
                     TQTARASKFLENSSVGVCTVIGFPFGVQTLYSKAFEVKDAIDNGANELDMVLNVGALH
                     SGDDNLVYRDIRGVVLAAGGKVVKVIFE"
     gene            complement(413..>1989)
                     /locus_tag="PKK85_06590"
     CDS             complement(413..>1989)
                     /locus_tag="PKK85_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNS25718.1"
                     /translation="KSRKDLQKLKLKVCRDFQLDKFPSNSSILKEATLPEQKWISPLL
                     KKKPTRTISGVAVVAVMCPPHQCPHGRCFYCPECDTAPPSYTGEEPAALRARMFDFHP
                     YRQVYNRLLQLESIGHPLDKVELIIMGGTFPSQPICFQEWFITRCIQAMNDFGGDRDK
                     MEVSTDDELDVPTYFRYLTDAQVDNERSRVRCVGMTFETRPDYSKKEDVDRMLQMGVT
                     RVELGVQTIYNFVYHRMERGHRVEDVVEANQVLRDSGLKVAMHFMPGLLSNPEMDLRI
                     FRRVFSDGRFMPDMLKIYPCLVTKGSRLHQNWEKGIYQPYSTADAVEVIVEIKKMLPK
                     WVRTMRIQRDIPAQLIEAGVKKSNLGELVYRRLEDEGSKCQCIRCREVGHQASRGIYP
                     DLDRVRLLREEYEAVGGREIFLSAEDPENDVLMGFLRLRMPSSAVHRSEIDVNTSLVR
                     ELHVYGPVVPLGERKVDAGQHRGYGEELLKEAERIAREEHEMKKMVITSGVGVREYYR
                     KFGYRKEGPYMSRSLD"
BASE COUNT          525 a          533 c          373 g          558 t
ORIGIN      
        1 tttcaaagat tacctttaca acttttccac cagcagccag caccacaccc cttatatcac
       61 gataaaccag attatcatcc ccagaatgca gtgcacctac atttaaaacc atgtccagtt
      121 cattagcacc attatcaatg gcatctttaa cttcaaaggc cttggagtag agcgtctgaa
      181 ctccaaatgg gaagcctatc actgtgcaaa cacccaccga actattttct aaaaacttgc
      241 tagcccgggc tgtttgggtg ggggttacac atacattcct aaatccatac aactctgctt
      301 catcacagag cttttctata tcattccatg tggcatccct gcctacattg gtatgatcaa
      361 ttttctgagc caattcctga gcatccatca tatcataata cctccaagat ttttaatcaa
      421 gtgatctgga catgtaaggg ccttctttac gatatccaaa ttttcggtag tattcacgca
      481 cccctacacc gctggtaatt accattttct tcatttcgtg ttcttcccgg gctatcctct
      541 ctgcctcctt taggagttcc tcaccatatc cacggtgctg cccggcatct acttttctct
      601 caccaagggg gaccacgggg ccgtaaacat gcagttccct caccagagaa gtgttaacat
      661 caatttcaga acgatgaaca gcgctggaag gcatgcgcag acgtaaaaat cccatgagaa
      721 catcattttc tgggtcttct gcagataaaa atatttctct accccctaca gcttcatatt
      781 cctctctcaa taacctaact cgatctaaat cggggtatat gcctcttgaa gcttgatgtc
      841 ctacctccct acaccttata cactgacact tgctaccttc atcttccaac ctgcgataaa
      901 ccaactcccc caagttagat ttttttaccc ctgcttctat gagttgagca ggaatatccc
      961 gttggatacg catggttcgc acccacttag gcagcatctt cttgatttcc acgatgactt
     1021 ccacggcatc ggcagtgctg tatggttgat aaatcccttt ctcccagttc tggtgtaaac
     1081 gggagccttt ggtaactagg cagggataaa tcttcagcat atctggcatg aacctcccat
     1141 cactaaacac ccgccggaag atgcgaagat ccatttcagg attggacaac aaacccggca
     1201 tgaaatgcat ggccaccttc aagccagagt cccggaggac ctgattagcc tccaccacat
     1261 cctccacccg atgccccctt tccatacggt gatacacaaa attataaatg gtttgtacac
     1321 ccagttccac ccgggtaaca cccatctgga gcatgcgatc cacatcttct tttttagagt
     1381 aatcaggcct tgtttcaaat gtcatgccca cacagcgcac ccgggatcgt tcattatcta
     1441 cttgagcatc agttaagtac cggaaatatg tgggaacatc cagttcatca tcggtggaaa
     1501 cctccatttt atctctatca ccaccaaagt cattcatagc ctgaatacag cgagttatga
     1561 accattcctg gaaacatatt ggttgtgaag gaaaagttcc acccatgatt attaactcta
     1621 ccttatccag tggatgacca atgctctcta actgcaagag acgattgtag acctggcggt
     1681 agggatgaaa gtcaaacatc ctggccctta aggccgctgg ctcctcacct gtataactgg
     1741 gaggggccgt atcacattct ggacagtaaa aacatcttcc atgtgggcac tggtgagggg
     1801 ggcacatcac tgccactact gccacaccag atatggtgcg ggttggtttt ttctttaata
     1861 atggtgaaat ccacttctgc tctgggagag tggcctcttt taggatggag gagttgctgg
     1921 gaaatttatc aagttgaaaa tctctgcaaa ccttcaattt tagtttttgg agatctttcc
     1981 tacttttaa
//
LOCUS       DAOQVW010000297         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_73068_length_1989_cov_2.947260, whole genome shotgun sequence.
ACCESSION   DAOQVW010000297 DAOQVW010000000
VERSION     DAOQVW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73068_length_1989_cov_2.947260"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="prf1"
                     /locus_tag="PKK85_06595"
     CDS             complement(<1..798)
                     /gene="prf1"
                     /locus_tag="PKK85_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HNS25719.1"
                     /translation="MADTSTREIYEVKKTLEELSRKKGRGTELVSVYIPPDRQISDVV
                     KHMREELSQSANIKSKQTKKNVQSAIEVIMQRMKLFPKPPENGLVLFVGMIPKGGPGT
                     EKMETYVFEPPEPVQTYIYHCDSQFFLEPLQEIIAEKDVYGLAVIDRKEATIAILRGK
                     RIDIVKNLTSGVPGKHKAGGQSQRRFDRLIELAAHEFLKRIGNHMNEAFLSIEGLEGV
                     IIGGPGHTKEDFVDGDYLHHEIKQKIITTVDTSYTGEFGIREVIEKSM"
     gene            940..1617
                     /gene="pyrH"
                     /locus_tag="PKK85_06600"
     CDS             940..1617
                     /gene="pyrH"
                     /locus_tag="PKK85_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNS25720.1"
                     /translation="MRIVITLGGSVIIRDHNPQLFQDYAQVFKDINKENDLFVVVGGG
                     KTARDYISIARGVGASEALCDDVGIDVTRLNARLLCIALGNKAYPRIPQNFHQAQEFA
                     TSGKIVVMGGTEPAHSTDAVGSILAEFVGADLLINATSVDGLYDKDPKKYQDAKLFPE
                     VTPSKVLECLSGNEVKAGTYEFLDHTAIQIIKRSGIKTIILNGENPENIKRALNERIG
                     TLIISED"
     gene            1639..1827
                     /locus_tag="PKK85_06605"
     CDS             1639..1827
                     /locus_tag="PKK85_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HNS25721.1"
                     /translation="MTDQHKHCPICGTPMPMNEKFCSPKCEQIYNVNQNKVKKTRRIL
                     YVAFTAFILVWIYYMMIR"
BASE COUNT          596 a          404 c          381 g          608 t
ORIGIN      
        1 catggacttc tcaataacct cccggatacc gaattcaccg gtgtacgagg tatccacagt
       61 tgttatgatc ttctgcttga tctcgtggtg taagtaatcc ccatcaacaa agtcttcctt
      121 ggtatgacct ggaccgccta taataacccc ttccaagcct tctattgata gaaatgcttc
      181 gttcatatgg tttcctattc ttttcaaaaa ttcatgggca gccagttcaa tcaaacgatc
      241 aaaacggcgc tgtgactgtc caccagcttt atgtttacct ggaactccac tggtcaggtt
      301 tttcacaata tcaatacgtt taccgcggag aatagcgatg gttgcttcct tacgatcaat
      361 caccgccagt ccgtagacat ccttttcggc aataatttcc tgcagtggtt ccaggaagaa
      421 ctgggagtca cagtggtaga tgtaggtttg cactggctct ggaggttcaa aaacataggt
      481 ttccatcttt tctgttccag gaccaccttt aggtatcata cccacgaata aaaccaatcc
      541 attctccggt ggcttaggaa aaagcttcat gcgttgcata ataacttcga tagccgattg
      601 aacattcttt tttgtttgtt tactttttat gttggcactt tgactgagtt cttcccgcat
      661 atgcttaact acatcactaa tctgacgatc cgggggtata taaactgaaa ccaattccgt
      721 acctctccct ttttttcgcg ataactcttc tagggtcttt ttaacttcat atatctccct
      781 ggttgatgta tcagccacta aaaatcactc ctttaatgaa gataagttgg aaagactaga
      841 ttctatttat tataaaaagg atgtttatta attgacatcc attagatatc ctttttaagg
      901 tttatatcct actttagtta aattatcttt ggtgatgtga tgcgaatagt aattaccctg
      961 gggggatcag tgatcatcag ggaccataac cctcaacttt ttcaagatta cgctcaagtt
     1021 tttaaggata taaacaaaga aaacgattta tttgtagtgg taggtggtgg aaaaacagct
     1081 cgagattaca ttagcatagc aaggggtgtg ggggcttcag aagcattatg tgatgatgtg
     1141 ggaatcgatg taacccgttt aaatgcccga cttctttgca tagccctggg aaacaaggcc
     1201 tatccccgaa ttccacaaaa ctttcatcag gctcaagaat ttgcaacttc tggtaagata
     1261 gtggtaatgg gaggtactga accagcccac agtaccgatg cggtggggag tattctggct
     1321 gagtttgtag gcgctgatct tttaattaac gccacctcag tagatggact ttatgataaa
     1381 gaccccaaaa aataccagga tgctaaactc ttcccggaag tcactccctc caaggttttg
     1441 gagtgtttat cgggtaatga agtgaaagca gggacttatg aattcttaga ccacactgcc
     1501 atccagatca taaaaagatc tggcatcaaa accatcatat taaatggtga aaacccagaa
     1561 aacattaaaa gggcccttaa tgaaagaata gggactttaa ttatttctga agattaatct
     1621 ggatgaggtt taaaaaaaat gactgatcaa cacaaacact gccccatatg tgggactccc
     1681 atgcccatga atgaaaaatt ctgttcacca aaatgtgagc aaatttataa tgttaaccag
     1741 aacaaagtta aaaaaacacg tagaatcctt tacgtagcgt ttactgcgtt tattttagtt
     1801 tggatatact atatgatgat tcgttaaaaa aaataaaggg tgaatctttt taaaaatttt
     1861 ttcattcttc agcggatatt gaggataccg tatatcaaaa atccagctcc gattattatg
     1921 cctagaatca atgggtctat ggcatagtaa cccactataa agtagattat ccccactagt
     1981 attcctatt
//
LOCUS       DAOQVW010000298         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_73285_length_1985_cov_3.500000, whole genome shotgun sequence.
ACCESSION   DAOQVW010000298 DAOQVW010000000
VERSION     DAOQVW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73285_length_1985_cov_3.500000"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKK85_06610"
     CDS             <1..794
                     /locus_tag="PKK85_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNS25722.1"
                     /translation="MVAGEGIHPSDVRIGGMADNISEVARKKLYARLKSLKPKLDEHV
                     ELIVGLVADKGLPAGLGVHNQPIFTTDNLYGNRDNFDMDRFTEVMPESWYDDADIAKR
                     ACSTIPLYDGVNVETGPRARAVQYRGFTEKGVVAQHVARAMEMKSALSRAIAILAELD
                     TSAPVRADFDPRGTNKLGIGPIEGPRGTDVHMAQVADGKVQFYTCLVPTTWNIPTMGP
                     ATEGFHHEFGPHVIRAYDPCLSCATHVIVVDDEDRSVIKNEMVRI"
     gene            804..1277
                     /gene="frhD"
                     /locus_tag="PKK85_06615"
     CDS             804..1277
                     /gene="frhD"
                     /locus_tag="PKK85_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase, FrhD
                     protein"
                     /protein_id="HNS25723.1"
                     /translation="MPYDAEIMVVGCGNILFADDGFGPAVIEALGKISEEHPLPEKVK
                     IVDAGTGGPHYVFSLPQDSWKKMIVVDVVEFGAESGTLRKFSVEELPKGSYENVHSWP
                     VNQPLHGLAESCEVMVIGCQPERISAPDIEMGLTKSVEEAIPKAIKLILEEIGVN"
     gene            1278..>1985
                     /gene="frhG"
                     /locus_tag="PKK85_06620"
     CDS             1278..>1985
                     /gene="frhG"
                     /locus_tag="PKK85_06620"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HNS25724.1"
                     /translation="MSILARIKSFLGLEAKPDKEAKPKQEASQQEVEKVAEEKAKPRI
                     GYIHLSGCTGDAISLTENYDILAELLTNMVDIVYGQTLVDLWEMPEMDLALVEGSCCL
                     QDEHSVKELMEVREKAALVCALGSCAATGCFTRYSRGGQQAQPGHESFVPIADLVKVD
                     CAVPGCPPSPEIIAKTVVALINGDMDYLQPMLDLAGYTEACGCDLQTKVVNQALCIGC
                     GTCAMSCQTRALSMTNGR"
BASE COUNT          595 a          423 c          493 g          474 t
ORIGIN      
        1 ttatggtggc cggtgaagga attcacccct ctgatgtccg gattggggga atggctgaca
       61 atatatctga agtagcccgt aaaaaactgt atgcaaggtt aaaatcttta aaacccaaac
      121 ttgatgaaca tgttgaattg atagttggac tcgtagctga caaaggcctg ccagcaggtt
      181 taggtgtcca taaccagccc attttcacca ctgacaatct atacggaaat cgggacaatt
      241 tcgacatgga tagattcaca gaagttatgc ctgaaagctg gtatgatgat gcagatattg
      301 caaaaagagc atgctccacc atacctcttt atgatggtgt gaacgtggaa acaggaccaa
      361 gagctcgtgc agttcaatac cggggattca ctgaaaaagg tgttgttgcc caacacgtgg
      421 ctagagccat ggagatgaaa tcagctttat ccagagctat agctatttta gctgaattgg
      481 atacttctgc accagtccgt gcggactttg atcctagagg aacaaacaaa cttggaattg
      541 gtcctattga aggtccaagg ggaaccgacg tgcacatggc acaagttgcc gacggcaaag
      601 tccagttcta cacctgtctg gtacccacca cttggaacat acccaccatg ggaccagcca
      661 ctgaaggatt ccaccatgaa ttcggccccc acgttatccg agcgtacgac ccatgtctgt
      721 cctgtgcaac acacgtgatt gtggtggacg atgaagacag gagcgtcata aaaaacgaaa
      781 tggtcagaat ctaagggata accatgccat acgatgcaga gatcatggtg gtcggatgcg
      841 gaaatattct ctttgcagat gacggattcg gaccagcagt gatagaagca cttggaaaaa
      901 tttctgaaga acatcctctt ccagagaagg tcaaaatagt agatgctggc actggaggcc
      961 ctcactacgt gttttctctt ccacaagatt cttggaagaa aatgatagtg gtagatgttg
     1021 tggagttcgg tgctgaatct ggtaccttgc gcaaattcag tgtggaagaa ctacctaaag
     1081 gatcctacga gaacgtgcac tcatggccgg taaatcagcc attgcacgga ttagcagagt
     1141 cctgtgaagt aatggtaatt ggatgtcaac cagaacgtat ctctgccccc gatattgaaa
     1201 tggggttaac caaaagtgtg gaggaagcta ttcccaaggc cattaagttg attttagaag
     1261 agataggggt taattaaatg tcaatattag cccgtattaa atcgttttta ggattggagg
     1321 caaaacctga taaagaagca aaacctaaac aagaagcttc acaacaggag gttgagaaag
     1381 tggctgaaga gaaagctaaa ccaagaatag gttacatcca cctttcagga tgtactggcg
     1441 acgcaatatc gctaaccgag aattacgata ttctcgcaga attactcacc aacatggtgg
     1501 acatagtcta cggacaaacc ttggtcgatc tatgggaaat gccagaaatg gacttagccc
     1561 tggtggaagg atcatgttgt ttgcaggacg aacacagtgt taaggaatta atggaagttc
     1621 gtgaaaaagc agcactggta tgtgcacttg gctcatgtgc tgccacaggt tgtttcaccc
     1681 ggtactcacg gggaggacag caagcacaac ctggacatga atctttcgta cccattgctg
     1741 atctggtcaa agtggattgt gccgtaccag gatgtccacc atcaccagaa ataatagcca
     1801 aaaccgttgt agcacttatc aacggtgaca tggactacct acaacccatg ttagacctgg
     1861 ctggttacac cgaagcctgt ggttgtgatc tgcagactaa agtggtaaac caggctctat
     1921 gtattggatg cggaacctgt gccatgtcct gccagacccg ggctctaagc atgaccaatg
     1981 gaagg
//
LOCUS       DAOQVW010000299         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_73293_length_1985_cov_3.096891, whole genome shotgun sequence.
ACCESSION   DAOQVW010000299 DAOQVW010000000
VERSION     DAOQVW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73293_length_1985_cov_3.096891"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKK85_06625"
     CDS             complement(<1..449)
                     /locus_tag="PKK85_06625"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNS25725.1"
                     /translation="MTMEINATINSSTYQHSTWGILIQDQNTSEVLFTENSQELFVPG
                     STTKILVAAAALAAYGPDHSFETPVYYDGELESGTLQGNLVLVASGDLTMGGRNTPDG
                     KLAYTNLDHCEANSMDGANLTSTDPLAGLNELARQVKASGVSRVEGDV"
     gene            complement(953..1417)
                     /locus_tag="PKK85_06630"
     CDS             complement(953..1417)
                     /locus_tag="PKK85_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNS25726.1"
                     /translation="MNIGIVVYSETDHTYSIAQKLQDKLSVEGHEVNLERVVPAVKVD
                     PGDLNVNFKSQPDTDPYEGLIFGAPVHAFNLAPAMNAYLNQIPSLKDKKIACFVTKGL
                     PFDSTGGNQAIKQMKRVCRDRGGIVMGTSIVTWSVSRKKKINNLLSQFGAMF"
     gene            complement(1672..>1985)
                     /locus_tag="PKK85_06635"
     CDS             complement(1672..>1985)
                     /locus_tag="PKK85_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS25727.1"
                     /translation="GALGDRRASEPLMEILKEDDYILRACTVTSLGKIGDNLSLQSLI
                     RSLEDENYLVRVNAAHALGTIGDKAALPYLQEALNKSRGESQEFERAIRDAMAQINTR
                     S"
BASE COUNT          484 a          470 c          465 g          566 t
ORIGIN      
        1 acatcaccct ccaccctgct gaccccggag gccttaacct ggcgtgccag ctcattaagt
       61 ccagcgagag gatcggtgga ggttaaatta gcaccatcca tggagttggc ttcacagtgg
      121 tccaggttgg tgtaggccag cttaccatcc ggggtgttgc ggccacccat ggtcaggtcc
      181 ccactggcca ccagtaccag attcccctgg agggttccgg attcaagttc accatcatag
      241 taaaccgggg tttcgaagct gtgatccgga ccgtaagcag ccagggcagc ggcagcaact
      301 aatatcttag tggtggatcc gggtacaaat agttcctggg agttttctgt gaagagtact
      361 tcgctggtgt tctggtcctg tatcaggatg ccccaggtgg agtgttgata ggtagatgag
      421 ttaatggtgg cgttgatttc catagtcatg ttgcttatgt taccaggggt ggtgttgtta
      481 acttcctgag atgctgtgaa gcttccagag gtggataaga atagtaaagc agaaattaag
      541 agtgttaaaa agatcttgta tactgcggaa atagaaaacc tcctgatatt gaatagtatt
      601 tcccacctaa tatttcaatt attctctagt agccccagaa ggttctatgc accaacatcc
      661 acgccgggag gttcaggacc atatttttga ggttgaagtc ccggtcctga tgaaatggac
      721 catcccacac cgcagaggtg aacctatgca gctggttaat tgagtgtcct tgtggtggct
      781 caaatacact atcgccttta ttttctgatt ctgtcttcaa ttaatggggt tcctgtatct
      841 gttcccccca ttccagggct tcccatcttg gctgagattt atgggagtaa ctaagtttag
      901 gaactcagca ggcatgtttt ttaacagaat aaacgtatcc atccacttca aattagaaca
      961 tagcaccgaa ctgagacaac aagttgttta tcttcttttt ccgactcaca ctccaggtta
     1021 caatggatgt tcccatcaca attccgcccc tatcccggca gactcttttc atctgtttaa
     1081 tagcctggtt cccaccggta ctgtcaaagg gcaagccttt ggttacgaaa caggcgatct
     1141 tcttatcctt tagggatggg atctggttta agtaggcgtt catggctggg gccaagttga
     1201 aggcatggac cggtgcccca aagatcaaac cctcataggg gtctgtatct ggctggctct
     1261 tgaagttcac attgaggtct cccgggtcaa ccttaacagc aggcaccacc cgttccaggt
     1321 tcacttcatg cccctctact gagagtttat cctgtagttt ctgggctatg gagtaggtgt
     1381 gatcagtctc ggaataaact acaatcccaa tgttcattgc caacatcacc cccaattctt
     1441 agcttagata actgatcatt aattatgttt tttcatgtat atcacaggtt caccaaaaaa
     1501 aatggatacc aaccttaatg aatagtaata tttttcatct aactggaagc tccctggtaa
     1561 taaaagaagg ttaaagcctg tttcctcctt gtggttcaac ttggaggatt tttcaggaag
     1621 gggactataa aaaacttccc ttccccatta catttacctt tggggctgtg gttaactacg
     1681 tgtattgatc tgggccatgg catctcggat ggcccgttca aattcctgag attctcccct
     1741 ggatttgttg agggcctcct gtaggtaggg gagtgcggct ttatccccga tggttcccag
     1801 tgcatgggca gcgttaaccc tcaccagata gttctcgtct tctagagatc taataaggga
     1861 ctgtaatgat agattatcac cgatcttgcc cagggaagtg acggtacagg cccggagtat
     1921 atagtcatct tccttcaata tttccatcaa gggctcagat gcccgcctgt cacctaaggc
     1981 cccca
//
LOCUS       DAOQVW010000300         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_73351_length_1984_cov_3.364956, whole genome shotgun sequence.
ACCESSION   DAOQVW010000300 DAOQVW010000000
VERSION     DAOQVW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73351_length_1984_cov_3.364956"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKK85_06640"
     CDS             complement(<1..561)
                     /locus_tag="PKK85_06640"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNS25728.1"
                     /translation="MKIIKLKDENRDKLLERSRIDAESVTKPVLEIINDVRENGDRSL
                     EHYTQKFDGVSLRDFKVKRREIDAAWEKVEPQVLEALEVAAQNISTFHKAQLPREWSI
                     KVGEGITAGQIIRPLERVGCYIPGGRAVYPSTILMTVIPARVAGVEEVICCTPPGQSG
                     KVNDIVLAAAHLAGAHQIYKVGGAQAI"
     gene            867..1202
                     /locus_tag="PKK85_06645"
     CDS             867..1202
                     /locus_tag="PKK85_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HNS25729.1"
                     /translation="MYKDELIQLHQFLVYVLKNLESDYEVKDECGEYLSLNISPHHIH
                     RTKAEHKHAIFVLSASISHVVATNNGGNSSNISNGLSELVKRSRKELIRSQNENSLKY
                     EKKYQEIML"
     gene            1229..>1984
                     /locus_tag="PKK85_06650"
     CDS             1229..>1984
                     /locus_tag="PKK85_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HNS25730.1"
                     /translation="MDGLRKMICLIDGEHYFPVTRNALETLDSLEHNEVVAAVFIGGT
                     EKLREATPAFIEEELGVPVYYDENFHDIPYQLITQSLMDHEPDVVMDLSDEPVVDYSK
                     RFKIASLVLEMGIPYEGPDFQFQPLDEHDLLIKPSLKILGTGKRIGKTAVSAYAARLI
                     DKEDYNPCVVAMGRGGPEEPEIVRGDQIEITPQYLMEQYNKGVHAASDHWEDALMSRI
                     LTIGCRRCGGGMLGQVFITNMKRGAQLANEVDAD"
BASE COUNT          545 a          423 c          466 g          550 t
ORIGIN      
        1 tatggcctgg gctcccccta ccttgtagat ctggtgggcg ccggccaggt gggcggcagc
       61 caggactata tcattcactt tcccactctg gcccgggggg gtgcagcata tcacctcttc
      121 cacaccagcc accctggcgg ggatgacggt catgaggatg gtggatggat aaacagcccg
      181 tcctccaggg atatagcagc ccaccctttc caggggccgt attatctgtc cagcagtgat
      241 tccctcccct accttaatgg accattccct gggaagctgg gccttatgga aggtgctgat
      301 gttctgggct gcgacttcca gggcctctag gacctggggt tccacttttt cccaggcggc
      361 atctatttcc cgccttttaa ccttgaaatc cctaagggaa actccgtcaa acttctgagt
      421 ataatgctca agactccgat ccccattttc acgcacgtca ttgataatct ccagaacagg
      481 tttagtaacg ctttctgcat ctatcctgga cctttccagg agtttatctc tgttttcatc
      541 ttttaattta atgattttca tattttcacc atataatatg tgctcttcga ggatagtacc
      601 taggatgtgg cttgattgtt attattactt cgatttttat gttgggggtc aaaagattta
      661 tttgtaatga taccaaaatt ataagatacg agtgcattgt attagataat gtttagaagt
      721 aactacccgc tgcggtactt tttttaatat gtaggtaagg atgaataggg tagtgtattg
      781 ggaataatac caattggtat tacataggta taaatgcaat accgaactaa tttgaaaacg
      841 ctttaaagta taggtgtctg gtagatatgt acaaagacga gctaatacag ctacatcaat
      901 ttctggtata tgttttaaaa aatctcgaaa gtgattatga ggttaaggat gaatgcgggg
      961 aatatctatc cttgaacata agtccacatc acatacaccg cactaaggcc gaacacaaac
     1021 acgctatttt tgttttatca gcctccatct cccatgtggt ggctaccaat aatggaggga
     1081 actcttctaa catctccaac ggcctgtcgg aactggtaaa gcgatcccga aaggaactta
     1141 tccgctccca gaatgaaaat tccttaaaat acgagaaaaa ataccaggaa atcatgttgt
     1201 aaagactcca gattgggatg tgaatattat ggatggatta aggaagatga tatgcctaat
     1261 tgatggtgag cactatttcc cggtaactag aaatgcctta gaaaccctgg atagcctgga
     1321 acataatgaa gtggttgctg ctgtttttat tggaggcaca gaaaaacttc gagaagcaac
     1381 accagccttt attgaggaag aattgggagt gcctgtttat tatgatgaaa attttcacga
     1441 tattccctac caactcatta cccaaagctt gatggatcat gaaccagacg tggttatgga
     1501 tctcagtgat gaaccggtgg tggactactc caagcggttt aagatagctt ctctggtgct
     1561 ggagatgggc attccctacg aagggcctga tttccaattt caacccttgg atgaacatga
     1621 tttattaata aaaccttccc tcaaaatcct gggaacgggt aaacgtattg gtaaaactgc
     1681 tgtttcagca tacgctgctc gcttaattga taaagaggat tacaatccct gtgtggtggc
     1741 tatgggtcgg ggtggcccgg aggagccgga aatagtccgg ggagatcaga tcgagattac
     1801 accccaatac ttaatggaac aatacaataa aggagttcac gcagcatcgg atcactggga
     1861 agacgccctc atgagtagga tactcaccat cggctgcagg cgatgtggtg gtggtatgct
     1921 gggccaggtg ttcatcacca acatgaagag gggggcccag ctggccaatg aagttgatgc
     1981 tgac
//
LOCUS       DAOQVW010000301         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_73585_length_1980_cov_2.312727, whole genome shotgun sequence.
ACCESSION   DAOQVW010000301 DAOQVW010000000
VERSION     DAOQVW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73585_length_1980_cov_2.312727"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKK85_06655"
     CDS             complement(<1..684)
                     /locus_tag="PKK85_06655"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HNS25731.1"
                     /translation="MNKYQLFAFFHNFYRIFPIKKNKISFIGWPEANFTGNLGHIQQE
                     FQKRNDFHYNFIFKDEYNMSAPLPILSKFMDLIRLFLLKSYHLATSHYIFLSDNFLPL
                     AYMNPSPETTIVQLWHASGAFKKFGLSTVSDEGLRELETKLSHKLDLITVSSKNVIPH
                     YQEAFGVEERKVKALGVPRTDFYLKNSNEEVLTRLRARFEKKYPALENKKIVLYAPTF
                     RDDPDRDQQL"
     gene            complement(698..>1980)
                     /locus_tag="PKK85_06660"
     CDS             complement(698..>1980)
                     /locus_tag="PKK85_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HNS25732.1"
                     /translation="SFKHKLYFILAEKLLSAHKAFIEPWNYRQFIKYFNRYTTPKKYI
                     FLQHGIIGNGVREFLEKRNPNNRFDLFICGAKPEFDYIDRDFGYLPGEVTYTGLARFD
                     HLNSESLKNQILLMPTWRAEIIQPSWIKKRVVDDETFLESEYFQRFQSIINNPKLMEL
                     LEKNDFKLIFYPHYEVQQYLHYFSGGNERVVLADKDSFDIQTLLLESKLLITDYSSVQ
                     FDFSYMEKPLVYYQFDKDFFFTKHYKKGYFSFEDHGFGPVLEEEEDVINYIEQEFKSD
                     FSVEKFYQERTNQFFGFRDHKNCERIYQEVMKLDHHYELVDDLVDEIFNNYTDQIKAP
                     GLDIYLHLPYLLYVDHTKKRTNNHFYLHLFDNSMKYMELDFFFSDLSVARDQRSKYTN
                     QNLAIVKMPGVNIKLIKTGLSINNEKQAFGEFII"
BASE COUNT          586 a          349 c          374 g          671 t
ORIGIN      
        1 cagttgctga tctctatctg ggtcgtccct gaaggtaggg gcatagagga ctatcttttt
       61 attttccagg gcaggatatt ttttttcaaa ccgggcccta aggcgggtta aaacttcttc
      121 gttactgttt ttcaaataaa aatctgttcg agggactcct agggccttta cttttctttc
      181 ttccacaccg aatgcctcct ggtagtgggg gattacattt ttggagctga cagtaatcaa
      241 atccagttta tgactgagtt tggtttcaag ttctcttaaa ccttcatcag aaactgttga
      301 gagaccaaac tttttaaatg cccccgatgc gtgccataat tggactatag ttgtttctgg
      361 agaggggttc atgtaagcca ggggtagaaa gttatcactg agaaagatgt aatgggatgt
      421 ggccaggtgg taagatttaa gtagaaacag tcggattaaa tccataaatt tacttaaaat
      481 aggtaaagga gcactcatgt tgtattcgtc cttgaagatg aagttgtaat gaaaatcatt
      541 ccgtttctgg aattcttgtt ggatatgtcc cagattcccg gtgaaattag cctctggcca
      601 acctatgaat gatattttat tttttttaat cggaaaaatc ctgtagaaat tgtgaaaaaa
      661 ggcaaataat tggtatttat tcataaaaaa ctcctatcta aattataaat tctccgaaag
      721 cctgtttttc attatttatg cttaaaccag tttttattag ttttatgttt acccctggca
      781 tcttaactat tgccaagttc tggtttgtat acttggatct ttgatcccgg gcaacactca
      841 agtcagagaa gaagaaatcc aactccatat atttcataga attatcaaat aggtgcaaat
      901 agaagtggtt gttggtcctc tttttggtgt ggtccacata caatagatag gggaggtgca
      961 ggtagatgtc cagaccaggg gctttaatct gatcagtgta attattgaat atttcatcaa
     1021 ccagatcatc taccaattca tagtggtgat ctaatttcat tacttcctga tatattcttt
     1081 cacagttctt atggtccctg aatccaaaga attggttggt tcgttcctgg tagaattttt
     1141 caaccgaaaa gtcactctta aattcttgtt ctatataatt aataacatcc tcttcctctt
     1201 ccagaaccgg accaaatcca tggtcttcaa aagaaaaata tcctttttta tagtgtttgg
     1261 tgaaaaaaaa gtccttatca aactgataat agaccaaggg tttttccatg tatgaaaaat
     1321 cgaattgaac actggaataa tctgtgatta agagcttaga ctccaggagt aaggtttgta
     1381 tatcaaagga atctttatct gccaacacaa ctctctcatt accaccagaa aagtaatgta
     1441 agtactgttg cacttcataa tgaggataaa atatgagttt aaaatcgttt ttttccagta
     1501 gttccattaa ctttggattg ttaatgatgc tctggaagcg ctgaaaatat tcagattcta
     1561 agaaggtttc atcatccacc accctctttt taatccatga tggttgtatt atctctgccc
     1621 tccaggttgg catgagcagg atctgatttt ttaaagattc gctgtttaaa tggtcgaatc
     1681 tggccagtcc agtgtaggtt acttcacccg ggagataacc aaaatctcga tctatataat
     1741 caaattcagg tttagcacca cagataaaca aatcgaaacg gttatttgga tttcttttct
     1801 ccagaaattc cctaactcca ttacctatga tgccgtgttg taaaaatatg tactttttgg
     1861 gagtggtata tctgttaaag tattttataa attgccgata attccatggt tcaataaaag
     1921 ctttatgggc ggataataat ttttcggcta aaatgaagta tagcttgtgt ttgaaactcc
//
LOCUS       DAOQVW010000302         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_73727_length_1977_cov_3.530697, whole genome shotgun sequence.
ACCESSION   DAOQVW010000302 DAOQVW010000000
VERSION     DAOQVW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73727_length_1977_cov_3.530697"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKK85_06665"
     CDS             complement(<1..595)
                     /locus_tag="PKK85_06665"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNS25733.1"
                     /translation="MNPEDLYQAMFRRKSVRNYHYSNLSRETLEEIESCLLKLTPLDP
                     HIKTEFRVVSGDQIKTRMMKRAPHYLLAFSEKREGHLVNVGFMLQQMDLYLSWRGIGT
                     CWQGIPQPKKSALATSTLGYDILLTMGKAQETLHRECVEEFKRMALEDITDIQGADDI
                     LEPVRLAPSSINSQPWHITGSPELMHFYCRKLNPMKSY"
     gene            662..>1977
                     /locus_tag="PKK85_06670"
     CDS             662..>1977
                     /locus_tag="PKK85_06670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNS25734.1"
                     /translation="MISWEEYQKLARELLERDIGGDDNPAQHRAVHAPASESQFLVAG
                     PGSGKTTVMVLKILKYIYVDSIPPQAIMATTFTRKAAGQLKSRIIKWGTKIKDHLLEE
                     AVFADLDPQVQDLELEGILTGTLDSLAQELLGGPLVLEEQASRSILMQKLIHDDLHRN
                     PDLLGYLKVMGRWQGTLNLTGICDLLLEIKDRILHDQVDCEALQADSEHPGAWLALNC
                     IREYREELERHQLYDFAGLEASLLGKLNQEEVPLDGLKVLLVDEYQDTNLLQESIYFH
                     LARIAIKNGGNITVVGDDDQSLYRFRGATVDLFTQYQRRIKEKVGVDVRVIHLSLNYR
                     STPQIVDFCNSFLEVDEDYQFARVRSKPSIQAARRDEDHYPVLGMFRRDRKTLARDLS
                     RFISQSLYRDGYQAEEMTIKLDKGDSSILMASPLEYTPHSARRFPH"
BASE COUNT          448 a          500 c          548 g          481 t
ORIGIN      
        1 gataggactt catagggttt aatttgcggc agtagaagtg catcaactca ggactcccgg
       61 tgatgtgcca gggctggctg ttgatggagg atggggccag tctgaccggc tccaggatat
      121 catctgctcc ctggatatcg gttatatcct ctagagccat tcttttgaac tcttcaacac
      181 attcccggtg cagggtttcc tgggcttttc ccatggtaag caggatatcg taacccagtg
      241 tagaggtggc cagtgcactt ttttttggct gcggtatacc ctgccagcag gtgcctattc
      301 cccgccagga taggtagagg tccatctgtt gcagcatgaa ccccacattc accaggtggc
      361 cctccctttt ttcggagaag gctaggaggt agtggggtgc tcttttcatc atcctggttt
      421 tgatctggtc cccggagact acccgaaatt cagttttaat atggggatct aggggtgtga
      481 gtttcaagag acagctttca atttcctcca gggtctccct ggagaggttg ctgtagtggt
      541 agttgcgtac actttttctc ctgaacatgg cctggtagag atcttcaggg ttcatggcat
      601 caccattttt tttagatagc tccctttttt tataagtatg gtggccctat attaagtcac
      661 aatgatcagt tgggaagagt atcagaaact ggcccgggaa cttctggagc gggacattgg
      721 aggggatgat aacccagccc agcatagggc tgtacatgcc ccggccagtg aatcccagtt
      781 tctggtggct ggtccaggga gtggtaagac cacggtgatg gtgctgaaga tcctcaagta
      841 catctacgtg gactccatac caccccaggc tatcatggcc accaccttca ctaggaaggc
      901 tgctggccag ctaaaatcca ggataattaa gtggggaact aagataaaag accacttgct
      961 agaagaggcc gtttttgcag atctagatcc acaagttcag gatctggaac tggagggaat
     1021 cctcaccggc accctggaca gcctggccca ggaactcctg ggaggccccc tggtcctgga
     1081 ggagcaggcc agccggtcca ttctgatgca gaagctgatt catgatgatc tgcaccgcaa
     1141 cccggatctt ctgggttacc ttaaggtgat gggacgctgg cagggaaccc tgaatttgac
     1201 cgggatctgt gacctgctcc tggagattaa ggaccgcatc ctccatgacc aggtggactg
     1261 tgaagccctc caggctgatt cagaacaccc tggggcctgg ttggccctga actgtataag
     1321 ggagtaccgg gaggaactgg aacgtcatca actgtatgac ttcgctggcc tggaggcctc
     1381 ccttctgggg aagttgaacc aggaagaagt ccccctggat ggtcttaagg tgctgcttgt
     1441 ggatgagtac caggacacca acctcctcca ggaaagtatc tacttccatc tggccaggat
     1501 tgccataaaa aatggtggta acatcaccgt ggtgggggac gatgaccagt ccctgtaccg
     1561 cttccggggg gctaccgtgg atcttttcac ccagtaccag aggcggatca aggaaaaggt
     1621 aggtgtggat gtccgggtta tacacttgtc cctgaactac cgctccaccc cacagatcgt
     1681 ggatttctgc aactccttcc tggaggtgga tgaggactac cagtttgccc gggttaggag
     1741 taagcccagt atccaggctg cccggaggga tgaagaccat tacccggttt tgggcatgtt
     1801 ccgccgggac cgtaagaccc tggcccggga tctatccagg ttcataagcc aatccctcta
     1861 cagggatggt taccaggccg aggaaatgac tattaaactt gataaaggag atagttccat
     1921 cctgatggcc agcccattgg agtacacacc tcacagtgcc cgtagattcc cccacta
//
LOCUS       DAOQVW010000303         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_74189_length_1969_cov_3.673981, whole genome shotgun sequence.
ACCESSION   DAOQVW010000303 DAOQVW010000000
VERSION     DAOQVW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74189_length_1969_cov_3.673981"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..360)
                     /locus_tag="PKK85_06675"
     CDS             complement(142..360)
                     /locus_tag="PKK85_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNS25735.1"
                     /translation="MFRDEYGSKGNSAPINEGEEYDVKIEDTGRDGDGITRIEGFVIF
                     VSGAKVGDEVKIRINSVRRNFGFAEVVE"
     gene            597..1247
                     /gene="pyrF"
                     /locus_tag="PKK85_06680"
     CDS             597..1247
                     /gene="pyrF"
                     /locus_tag="PKK85_06680"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNS25736.1"
                     /translation="MDVKNRIIVALDLPDLEKAIEVVDAVKKEINTVKIGYPLVLGQG
                     LDSISTIKNEFSCQVIADFKVADIPETNRKIVDLTFQAGADAVIVHGFVGADSAQACM
                     DSAHKNGGEVFLLTEMSHPGALKFIKPVSLEIARMGVKLGIRNFVGPSTRLDRLEKIR
                     RIIGSGSFLISPGVGAQGGNPKDTLDFADALIIGRSVYLSEDPEFAIESFIDSIEK"
     gene            complement(1191..1781)
                     /locus_tag="PKK85_06685"
     CDS             complement(1191..1781)
                     /locus_tag="PKK85_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25737.1"
                     /translation="MLIEAIFYPLAGFFMKISDDALDEKKNMGLAVFSGLICVLAIGY
                     LSVNSSDAATIFIGIMVGTFLSGKVDRLAHIITLIIFLSILVIFGIPPLGIIALLICT
                     IAAYLDEMGNDSSWLKSKKKLNIFFKYRLALKLAVLFLAMGGLIQYLHPEYSGIILFQ
                     PETFIYFLLFDLAYEVAGLLFNTIYEGLNGKFGIFG"
     gene            complement(1825..>1969)
                     /locus_tag="PKK85_06690"
     CDS             complement(1825..>1969)
                     /locus_tag="PKK85_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNS25738.1"
                     /translation="ETGSLSGAPLEEAKSWAKARAGSNLVTVIGDATIIFPLMVAGAR
                     EIM"
BASE COUNT          633 a          388 c          367 g          581 t
ORIGIN      
        1 tagtttcgtc atccttcatc agatatccac tggaaatttt cgttgaaatt ttgatcagca
       61 attaaattaa aaataaacaa atataggata gttctgatat tttctaaaaa aagatgcaaa
      121 aaaataaatt gtggaataaa cctattccac tacttcggca aaaccgaagt tccttctgac
      181 agagttaatc ctaattttaa cttcatcgcc aaccttggca ccagaaacga atatcacaaa
      241 accttctata cgagtgatcc cgtcaccgtc cctaccagtg tcctcaattt taacatcata
      301 ttcttctcct tcattaatag gagcagaatt acctttacta ccgtattcat ctctaaacat
      361 attaaatcac tttccaaatt agccatgaca aatggcttaa cttaacgtat aaatttcgcc
      421 acgtatatac tttgtgtgaa gataagtgga ttttttaaaa aaaacttgtt attttggttt
      481 gtagatttaa taaagattat ttccaccaaa atttttaggt gaccatctca taatatgttc
      541 ttctaagttc atccggattt caaaaatcat attaaatctt gtgaacaagt aagaacatgg
      601 atgttaaaaa tagaatcata gttgctctag acctcccgga cttggaaaaa gccatagaag
      661 tggtggatgc tgttaaaaaa gaaataaaca cagttaaaat aggatacccc ctggttttag
      721 gacaggggct ggattccatt tccaccatta aaaacgaatt tagttgccag gttattgcag
      781 attttaaagt ggccgatatt cctgaaacta accgtaaaat agttgattta actttccaag
      841 ctggtgctga tgctgttatt gttcacggtt tcgtgggggc ggacagtgcc caagcttgca
      901 tggattctgc acataaaaat gggggggaag tatttttatt aaccgaaatg tcacatcccg
      961 gcgctttgaa gtttataaag cctgtatcac tagaaatagc aaggatgggt gttaaattag
     1021 gtatcaggaa ttttgtggga ccatcaacaa ggctggatcg cttggaaaaa attagaagaa
     1081 tcattggatc aggctctttc ctcatatctc ctggagtggg agcccaggga ggaaatccaa
     1141 aagatacttt agattttgca gatgcactca tcattgggcg cagcgtttac ctatccgaag
     1201 atcccgaatt tgccattgag tccttcatag atagtattga aaagtagacc agccacttca
     1261 taggccaaat cgaaaagaag gaagtagatg aacgtttccg gctggaaaag aataattccc
     1321 gagtattcag gatgtaggta ttgaattaat ccacccatgg ccaggaaaag taccgctaac
     1381 ttaagggcta atcgatattt aaaaaagata ttaagtttct ttttactttt caaccatgaa
     1441 gaatcgtttc ccatttcatc tagatatgct gcgatagtac atatcagtag ggcaatgatt
     1501 cccagtggag ggattccaaa tataaccagg atggaaagga atatgatcag ggtgatgatg
     1561 tgtgccagcc gatcaacctt ccctgacaaa aatgtgccca ccattattcc aatgaatatg
     1621 gtggcagcat cactgctgtt aactgacaag tagcctatgg ccagtacaca tattaaacca
     1681 gaaaaaaccg ctaatcccat gtttttcttc tcatccaggg catcatcaga aattttcatg
     1741 aaaaatccgg ccagcggata aaaaatagcc tcaattaaca ttttatcttt ttaaagatca
     1801 cgggggatcc atttttttga ttctttacat aatttccctt gcaccggcta ccatgagtgg
     1861 gaatatgatg gtagcatcgc caataaccgt taccagattg gatcctgccc tagccttggc
     1921 ccaggatttt gcctcctcca agggagcgcc acttaaactt ccagtttca
//
LOCUS       DAOQVW010000304         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_74404_length_1966_cov_2.857666, whole genome shotgun sequence.
ACCESSION   DAOQVW010000304 DAOQVW010000000
VERSION     DAOQVW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74404_length_1966_cov_2.857666"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKK85_06695"
     CDS             <1..648
                     /locus_tag="PKK85_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HNS25739.1"
                     /translation="SGAVKTEKNDSNALYLLIPAIGIEQTINNKSVDYGVYHEPQSSR
                     PGNGTVVLFGHRTLHGSPFLKLDQLKEGDNVTLVWSGVGNVEYRVTNSSIVSEDYRLS
                     VEQGHVLFLITCYPLGSTKQRLVIKAQQEHIYPYQAPGDTPEDVQNYCALALIAGFFV
                     GGMVVSLFYPIQEERYILTLATVALTMFLVMGYLFPTPAVGVESYISNLSNYLGV"
     gene            651..1022
                     /locus_tag="PKK85_06700"
     CDS             651..1022
                     /locus_tag="PKK85_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HNS25740.1"
                     /translation="MCSGKDEKYFKNISPRERAIFEGAITMGALFHQFIGTPVSESSV
                     KSLEQAIKESMELQPCIDAVEVNIDFGNSGGGNEYEYVSLTGEMLDIKVVSSYQGVRA
                     VTRMRYYEELNYPLMYVESVD"
     gene            1130..1339
                     /locus_tag="PKK85_06705"
     CDS             1130..1339
                     /locus_tag="PKK85_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25741.1"
                     /translation="MITIDKKLCKGCNICTEFCPHQVYEESGTLNKKGVHVPNPEHEE
                     RCTKCQVCSLLCPDQAIQVDEDDES"
     gene            1329..>1966
                     /locus_tag="PKK85_06710"
     CDS             1329..>1966
                     /locus_tag="PKK85_06710"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNS25742.1"
                     /translation="MKAENLFIPGNEACARGAVKAGCRFFAGYPITPSTEIAEDISLF
                     LPREGGTFIQMEDEISALGAVIGAVWGGLKGMTATSGPGFSLMQEHIGYAVMTETPLV
                     IINMQRGSPSTGQPTMASQSDMMQARWGSHGDYEIIALSPSSVQECFDFTVEAFNLAE
                     EYRVPVMVMSDEIVGHMREKITIPGKVTIKPRKMPSESPPDYLPYMAAPDGT"
BASE COUNT          552 a          402 c          489 g          523 t
ORIGIN      
        1 tctggggcag taaaaactga aaaaaacgat agtaatgccc tttacctgct aatacctgcc
       61 attgggatag aacagactat aaacaataaa tccgttgatt atggggtgta ccacgaacct
      121 cagtcatccc ggcctggcaa tggaacagtg gtcttgtttg gccatagaac tcttcatggg
      181 tctccttttc taaaattgga ccagttgaaa gagggtgata atgttacatt ggtttggagt
      241 ggagtgggta acgtggagta ccgggtgaca aattccagta tagtatcgga agattaccga
      301 ctttccgttg aacaaggcca tgtccttttc ctgatcactt gttaccccct aggatccact
      361 aagcaaaggc tggtgataaa ggcccagcaa gaacatatat atccctacca ggcaccgggg
      421 gacactccgg aggatgttca aaactattgt gccctggcat taatagctgg atttttcgtt
      481 ggcggaatgg tggtaagttt attctacccg attcaggagg aacgttatat tctgaccttg
      541 gccacagtgg ccttgaccat gttcctggtg atgggttacc tattccccac accggcagtg
      601 ggtgtagagt cctatatatc aaatcttagt aactatttag gggtgtaaga atgtgtagtg
      661 ggaaggatga gaaatatttc aaaaacattt ccccccggga acgagcaata tttgaagggg
      721 ccattactat gggggccctc ttccatcagt ttataggaac tccagtcagt gaaagtagtg
      781 ttaaatcact ggaacaagct attaaagaat ccatggaact tcaaccgtgc atcgatgctg
      841 tggaagtgaa cattgatttt ggaaactcag gagggggtaa tgaatacgaa tatgtttcac
      901 ttaccgggga gatgctggat atcaaggtgg tgtcctctta ccaaggagtg agggcggtta
      961 ccaggatgcg ttactacgaa gaacttaatt atccattaat gtacgttgaa agtgttgatt
     1021 aatccctatt attcttttta atccaattta tgatggttta atagccagct taatctcacg
     1081 gaaacaatct atttaaaata ccatcactaa aagaaaaatg gttgatgtca tgattactat
     1141 agacaagaag ttgtgcaagg ggtgcaacat ctgcactgaa ttctgtcctc accaggttta
     1201 tgaggaatca ggaaccctga acaagaaggg ggtgcatgtc cccaacccag aacatgaaga
     1261 acgatgtacc aagtgtcagg tttgctctct tttgtgtcct gatcaggcaa tacaggtgga
     1321 tgaagatgat gaaagctgaa aatctattca taccaggcaa cgaggcttgt gcccgcggag
     1381 cagtgaaagc tggctgtcgt ttttttgccg gttaccccat caccccatcg acggaaatag
     1441 ccgaagacat atcccttttt ctaccccgag agggaggaac atttatacag atggaagatg
     1501 agatttctgc actgggcgcg gttataggtg cagtttgggg cggtttgaag ggaatgaccg
     1561 ccacttctgg cccggggttt tctttaatgc aggagcacat tggctacgca gtgatgactg
     1621 aaactcccct ggtaataata aatatgcaga ggggttctcc ctccactgga cagcccacca
     1681 tggcttctca gagtgatatg atgcaagcca ggtggggttc gcatggagac tacgagatca
     1741 ttgctttatc cccttcatcc gtccaagaat gtttcgattt cactgtggaa gcatttaatt
     1801 tggctgaaga gtatagggtt ccggtgatgg tgatgagtga cgagatcgtg ggtcacatga
     1861 gggagaaaat caccataccc ggaaaggtga cgattaaacc gcgaaaaatg ccatcagaat
     1921 ctcctcctga ttatctacca tacatggctg ctccagatgg aaccag
//
LOCUS       DAOQVW010000305         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_75136_length_1954_cov_3.686151, whole genome shotgun sequence.
ACCESSION   DAOQVW010000305 DAOQVW010000000
VERSION     DAOQVW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75136_length_1954_cov_3.686151"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKK85_06715"
     CDS             <1..846
                     /locus_tag="PKK85_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25743.1"
                     /translation="ISPRTDNTKMVNVMYERIRAIKGKTLKQYNWIKDCELSKIRNIV
                     RFLKEMEGVVIVNPYPDVLQKIIEKSPFIKRDYQKIMMLAESITLLNYNDRKKWESDE
                     GTYIITSKQDIMFLYQLLENYMDSIFLNVPNSLLALHKRLTEIYGMDIEFTVADVRND
                     IDARTHGDIAGELRRLHKAGLLSVGEEKAAHGMNSYNVVSTKPTKIDPDVDLVLSEER
                     KRVLSYEHSPEFLAFVVEHENHVPILDIHSWGGVDFNPPPWEFFENNRKVKSVKLGDF
                     DDSEF"
     gene            981..1139
                     /locus_tag="PKK85_06720"
     CDS             981..1139
                     /locus_tag="PKK85_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25744.1"
                     /translation="MMNCEYCGAPLKPWWDKDSGLYRCTNPECLDPEYLANPLCRTVQ
                     KPRKEEEE"
     gene            1142..1522
                     /locus_tag="PKK85_06725"
     CDS             1142..1522
                     /locus_tag="PKK85_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25745.1"
                     /translation="MDELYIWMCREATEIQELRPESSYFPPVKDGEVVSGIMGDRWVS
                     YDTSMEQYRLLLWLPRQEDLQELIIDQTHLIPARILMLIYDEFEFIPVHLPEKSKIGI
                     ATLQVFMAVMYNKVWNGTTWETIK"
     gene            1519..1755
                     /locus_tag="PKK85_06730"
     CDS             1519..1755
                     /locus_tag="PKK85_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0034258 - nicotinamide riboside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HNS25746.1"
                     /translation="MTHNKGDVPVLFWVATALGLLGTILNSLQRIEGFLLWMVSNPLL
                     CWQSYRTGNVNMALMFLVYFSLSIVGVFTWSGYL"
BASE COUNT          574 a          315 c          502 g          563 t
ORIGIN      
        1 atcagccccc gtacggataa taccaagatg gttaatgtga tgtatgaacg gatcagggct
       61 attaaaggga agacgttaaa gcagtacaat tggattaagg attgtgaact tagcaagata
      121 aggaatattg tcaggttctt aaaggaaatg gaaggggttg ttattgtgaa tccttatcct
      181 gatgttttgc agaagataat tgaaaaaagt ccatttatca agagggatta tcagaagata
      241 atgatgcttg ctgagagtat aactttactg aattataatg accgtaaaaa atgggagagt
      301 gatgaaggaa cttatattat cacgtctaag caggatatta tgtttcttta tcagttgtta
      361 gagaattata tggatagtat cttccttaat gtcccgaata gtctccttgc tttgcataaa
      421 cgtttaactg agatttatgg aatggatatt gagttcacag tggccgatgt taggaatgat
      481 attgatgcac gtactcatgg ggatattgca ggagaactgc gtcgtttgca taaggcaggg
      541 ttgttgagtg ttggggaaga gaaggcagct catgggatga atagttataa tgtggtttct
      601 actaaaccta ctaaaattga tcctgatgtt gatcttgttt tgagtgaaga gaggaaacgg
      661 gttttaagtt atgagcatag cccggagttt ttggcttttg ttgttgagca tgagaatcat
      721 gttcctattc ttgatattca tagttggggg ggtgttgatt ttaatcctcc accttgggag
      781 ttttttgaga ataatcgtaa ggtgaagagt gttaagttgg gggattttga tgattcagaa
      841 ttttagaatt attagaattt cagaattttg gggggggtct gctgagagag gcggtttagt
      901 gttagatata ccccccggga attctgccat tctggtaata agtaatatca ttaaaagtat
      961 taaagaggag gcgatttaga atgatgaatt gtgagtactg cggggcgccc ctgaagccct
     1021 ggtgggataa ggacagcgga ctctaccgat gcacaaaccc ggagtgcctg gaccccgagt
     1081 acttggctaa tccattgtgc aggacggtgc aaaaacccag aaaagaggag gaggagtgag
     1141 tatggatgag ttatatatct ggatgtgccg ggaggccact gagatacagg aattaagacc
     1201 agaaagtagt tatttccctc cggtgaagga tggggaagta gtttctggta taatgggtga
     1261 tcggtgggta tcatatgata catcaatgga acagtacagg ttgttgcttt ggcttcccag
     1321 gcaggaagac ctccaagaac taataatcga ccaaacccac ttaattcccg ccaggatttt
     1381 gatgttaatc tatgatgagt ttgaatttat acccgtacat ctccccgaaa aaagcaaaat
     1441 aggtatagcc acccttcagg ttttcatggc agtgatgtat aataaggtgt ggaatggcac
     1501 cacctgggag acaataaaat gactcataat aaaggtgatg tcccagtcct gttctgggtt
     1561 gccacggcct taggcctcct cggaaccatc ctcaacagcc tccaaagaat agaaggattc
     1621 ctcctatgga tggttagtaa tccccttttg tgctggcaat catacaggac ggggaatgtg
     1681 aatatggcct tgatgttcct cgtgtacttc agccttagta ttgtgggggt tttcacctgg
     1741 agtggatacc tatgactcct gaaactaaaa tctggagatg ggcacggaac actaaagtta
     1801 gacataaaca acgagggttc caagttaaaa tgactcaaaa cgaactatat aaactcggcc
     1861 tccaagccta caaagatgga tgcccaattt gtggcagtgc atttgaagta ggctgtacac
     1921 ctgtggcaat taatagttta accttggatg tagt
//
LOCUS       DAOQVW010000306         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_75346_length_1951_cov_2.759494, whole genome shotgun sequence.
ACCESSION   DAOQVW010000306 DAOQVW010000000
VERSION     DAOQVW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75346_length_1951_cov_2.759494"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKK85_06735"
     CDS             complement(<1..214)
                     /locus_tag="PKK85_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HNS25747.1"
                     /translation="MKTTVSVIKADVGSIAGHGLAHPALLEKCKEILGKAKEEGLLED
                     FYVTNCGDDTELIMTHRNGEENEEIHE"
     gene            complement(451..1446)
                     /locus_tag="PKK85_06740"
     CDS             complement(451..1446)
                     /locus_tag="PKK85_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNS25748.1"
                     /translation="MNRICVIPGDGIGPEVMEAALTVLKAMELDMEYYPALAGNACFQ
                     KLGTTIPSQTVDQAQQAHATLFGAVTTVPGQNSAILTLRKKLGLYANLRPVKSYPGID
                     AIYPDLDFLIVRENTEGLYAGWEEYTPVGAQALRVVTRKASQRICEFAFNQALENGFK
                     RVTAVHKANILKKTDGIFRDTFYQVAENYPELEAEDLYVDVTARLLVKNPQHFQVLVT
                     TNLFGDILSDEGAGLVGGLGMVPSANIGDSNALFEPVHGSAPDIAGQGVANPTAMLLS
                     VVMMLHHLKEEKAANKLEKALLIVLKERRFLTPDLGGNSSTLRMAREVQDNLDAL"
     gene            1563..>1951
                     /locus_tag="PKK85_06745"
     CDS             1563..>1951
                     /locus_tag="PKK85_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS25749.1"
                     /translation="MEVQKNVERLIQTLKDEDELVQIQSISLLEEIGKPAVEQLISAL
                     DDGNKNVRKGAARALGEIGDTKAIEPLIDTLSDENKWVRREASTALSKMGEEVVDPLI
                     RILEDDDWRVRGAAAWALGNVRDNKAL"
BASE COUNT          513 a          450 c          457 g          531 t
ORIGIN      
        1 gttcgtgtat ttcctcgttt tcttcaccgt tgcggtgggt catgatgagt tcggtgtcgt
       61 caccacagtt ggtgacataa aaatcttcca ggagtccttc ttccttggct ttgcccagga
      121 tctctttaca tttttcgagt aatgcaggat gggccagccc atgccctgcg atacttccaa
      181 catctgcttt tattacacta acagtagttt tcatagcaca atacctccaa ataaatctgt
      241 aacagttcta aaacagcatt aaaaagctga agggtggcca tattagagtt atactctgtg
      301 aaattgtttt ttatgttttt ttaaagtggg agtgatcttc aatttgttaa atgacccaaa
      361 ggcagctttt agctgtgctt tttaaccctt tagtatggtg aactatattg tgtggtgcat
      421 ataaaattta ctactcacaa tactagatgg tcaaagagcg tctaagttat cctgtacctc
      481 cctggccatc ctcagtgtag atgaatttcc ccccagatca ggtgtgagga accgtctctc
      541 ttttaaaact attaacagtg ctttttcaag tttattagct gctttctctt ctttcaggtg
      601 gtgaagcatc atcaccacgg agagtaacat ggcagtgggg ttggccaccc cctgaccagc
      661 gatgtccgga gccgatccat gtaccggctc aaacagagca ttactatcac caatatttgc
      721 agatggcacc atgcccaggc ctcccaccag tcccgcgccc tcatcggaga gtatgtcccc
      781 aaagaggttg gtggtgacca gcacctggaa gtgctgggga tttttaacca gaagcctggc
      841 cgtgacatcc acgtaaaggt cctcagcttc cagttcaggg tagttctcag ctacctggta
      901 gaaggtgtcc cggaagatcc cgtcggtttt ttttaaaatg ttggccttgt gaactgcagt
      961 aactctttta aacccatttt ccagggcctg attgaatgca aattcacata tcctctggga
     1021 ggcttttcta gtcactaccc gcagggcctg cgcccccacc ggggtgtact cctcccagcc
     1081 ggcatagagt ccctctgtgt tctcccgtac gatcagaaag tccaggtccg ggtagatggc
     1141 atcaatacct ggataagatt taacgggcct caagttagcg taaaggccta acttttttct
     1201 aagggttaaa atagcactat tctgcccagg tactgtggta accgccccga aaagggtggc
     1261 atgggcctgc tgggcctggt caacagtctg agaaggtatg gtggttccca atttctggaa
     1321 acaggcgtta ccagctaggg caggataata ctccatatcc aattccatag cctttaaaac
     1381 tgttaaggcc gcctccatca cctcaggtcc aatgccgtcc ccgggtatca cgcatatacg
     1441 gttcatggta gattccttca ttttcattcg cacaaactat atttagaaaa aaatacataa
     1501 taaatcttcc aaccaagtta cttttaaacc atactgttta tttaagcatg ggaggtagga
     1561 ttatggaagt acagaagaat gtggaaagac ttattcaaac cttaaaagat gaagatgaac
     1621 tggtgcagat acagtccatt tctctactag aagaaatagg aaaaccagct gtggaacaac
     1681 ttataagtgc cctggacgat gggaataaaa acgttagaaa gggcgcagcc cgggcgttgg
     1741 gagaaatcgg cgataccaaa gccatagaac ccctaatcga tactctcagt gatgagaata
     1801 agtgggtccg tagggaggcc tccactgccc tgagtaagat gggtgaggag gttgtggatc
     1861 ccctgatccg gatcctggag gatgatgatt ggagggttcg gggagcagca gcctgggccc
     1921 tgggaaatgt aagggataat aaagccctga a
//
LOCUS       DAOQVW010000307         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_75700_length_1945_cov_3.872487, whole genome shotgun sequence.
ACCESSION   DAOQVW010000307 DAOQVW010000000
VERSION     DAOQVW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75700_length_1945_cov_3.872487"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKK85_06750"
     CDS             <1..600
                     /locus_tag="PKK85_06750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS25750.1"
                     /translation="AGREKEIGFVGSKTFFYDDKNVLQAAGGGYIDLLKGESHEVGFK
                     ELDKGQHDRIYELDYVGGSCMLVKRNLIDKIGLLDPKFFMYWEDVDWCYTGREHGYKS
                     VHAYKSRIWHKYGTSSENYLKTYYHNRNRLYFTRKHADKKVYQKFLKHYLKDVALESG
                     YQLICQRNWAMFKALISGTVQGFRLKPGDGKSNIELHGV"
     gene            627..1823
                     /locus_tag="PKK85_06755"
     CDS             627..1823
                     /locus_tag="PKK85_06755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS25751.1"
                     /translation="MKIAVFHNLPSGGAKRALYGYLDYLDKGGHEVDVYVPSTADEEF
                     MPLKNVSRNLKIFPVKKTRTGSLYSSLQYVPPLIKSISLWDLEKTQKEIAETINSADY
                     DLVFSEQDQFTMTPFFLKYIKGPTVYYCPQPPRNEAILEAVSHRKQEINPLKRLLFNY
                     LDNKDWKLDKNNMSYAKYILTNSYFTRESILRVYGVNSYVSYLGVDTSLFQPQEVSEE
                     NFVLSVGSCKPSKGYDFLTRSLALIDPEIRPKLVLVSNFSEPGWEDYLQKLATHLDVN
                     LDILHLVNDEKLVSLYNKAKLVLYAPYLEPFGLVPLEAMSCGTPVVAVKEGGVRETVI
                     HKKTGLHTERDELLFAEATQKLLTSAEKRDEMSKNSIKTVQEYWTLKHAGDRLIWHLN
                     RLVNGN"
BASE COUNT          622 a          376 c          431 g          516 t
ORIGIN      
        1 gctgggaggg aaaaggagat cggatttgtt ggatccaaga cctttttcta tgacgataaa
       61 aatgttttac aagcagctgg cgggggctac atcgaccttt tgaagggcga atcccatgaa
      121 gtgggcttta aagaattgga taaaggtcag catgatagga tctatgaact ggattatgtg
      181 ggggggtcct gtatgctggt gaaaagaaat ctaatagata agatcggtct tctagatccc
      241 aagttcttca tgtactggga agatgtggat tggtgctaca ccggtagaga acatggatac
      301 aaatcagtgc atgcctacaa gtcccggata tggcataaat acggaacctc cagtgaaaac
      361 tatttaaaaa cttattatca taatcgaaat aggctttatt tcacacggaa acacgcggat
      421 aaaaaagtct atcaaaaatt tttaaaacac tacctgaagg atgttgccct ggaaagcgga
      481 taccaactaa tttgtcaacg caattgggcc atgttcaagg ctttaatcag tggaactgtc
      541 cagggattta ggctaaaacc cggagatgga aaatctaata ttgaattaca tggagtataa
      601 aaagaggtta agggttgaaa atgggtatga aaatagcggt attccataat ttaccatctg
      661 gtggggctaa acgggccctt tatggttatc tagattacct ggataagggt gggcatgagg
      721 tggatgttta tgtcccctcc actgctgatg aagaattcat gccccttaaa aatgtatcca
      781 ggaacttaaa aattttccct gtaaaaaaaa ccagaacagg gtccttgtat tcctccctcc
      841 aatatgttcc accattaatt aaatccatat ccctgtggga tttagagaaa actcagaaag
      901 aaatagcaga aaccattaat tcagctgatt acgacttggt cttcagtgaa caggaccagt
      961 tcaccatgac tccttttttt ttgaagtaca taaaaggacc aacagtttac tactgtcccc
     1021 agcctccacg taatgaagca attctagaag cagtatccca ccggaaacag gaaataaatc
     1081 cattaaaaag gttactcttt aattatctgg ataataagga ttggaagctg gataaaaata
     1141 atatgtccta tgcaaaatac atcctgacca actcctactt cacccgggag tccatactaa
     1201 gggtgtatgg agttaactcc tacgtatcct acctgggagt tgataccagc ttgttccagc
     1261 ctcaagaagt ttctgaagaa aattttgtgc tctcagtggg cagctgcaag ccatctaagg
     1321 gctatgattt tttaacacgc tcgttagcat taatagaccc tgaaatacga cccaaactgg
     1381 tcctggtctc caacttctcg gagcctggat gggaggatta cctccaaaaa ctggccaccc
     1441 atttggatgt gaacctggac atcctccatc tggtgaatga tgaaaaactt gtttcacttt
     1501 acaataaagc caaattagta ctttacgcac catatttaga accatttggc ctggtgcctc
     1561 tggaagccat gagttgtggc actccagtgg tggctgtgaa ggagggtggt gtcagggaaa
     1621 cggtgatcca caaaaaaaca ggattacaca ccgagcgtga cgaactcctc tttgctgaag
     1681 caacccagaa acttttaact agtgcagaaa aaagagatga aatgtcaaaa aacagtataa
     1741 aaacggtgca ggaatactgg accctaaaac atgccgggga cagacttatc tggcatttaa
     1801 atcgattggt caatgggaac tagttagcag cccaatattt tttcaacata aaactaaaaa
     1861 atcagtgata tcatgatccc caggataact gtagttgttc ttaattggaa tggttgggag
     1921 gatactgtgg agtgtttgga gtcgt
//
LOCUS       DAOQVW010000308         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_75832_length_1943_cov_3.187500, whole genome shotgun sequence.
ACCESSION   DAOQVW010000308 DAOQVW010000000
VERSION     DAOQVW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75832_length_1943_cov_3.187500"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKK85_06760"
     CDS             complement(<1..222)
                     /locus_tag="PKK85_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS25752.1"
                     /translation="MSTTSRREREKQKRRQDIINAAEKIFFAKGYDSVTMDEIAREAE
                     INKALIYYYFKNKEALFFAVNLYGVRILHA"
     gene            466..975
                     /gene="mtrA"
                     /locus_tag="PKK85_06765"
                     /pseudo
     CDS             466..975
                     /gene="mtrA"
                     /locus_tag="PKK85_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
     gene            1361..1807
                     /locus_tag="PKK85_06770"
     CDS             1361..1807
                     /locus_tag="PKK85_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNS25753.1"
                     /translation="MVEINEKAPVLAKAQIEIKATPETVWKIMADIEAWPKWNSDVKK
                     ACLKGELKEGTQFQWKTKVGKITSLLQKVEPPHLLAWTGRIMGINAIHVWRIQTKNKK
                     TQVITEESWEGALSRVMSGKMQKMLEESINSGLQYLKTEAERLSDS"
BASE COUNT          666 a          367 c          397 g          513 t
ORIGIN      
        1 ggcatgcaat attcttactc cataaagatt cactgcaaaa aatagggctt ctttattttt
       61 aaaataataa tagatgagtg ctttgttaat ttctgcttcc cgggcaattt catccatggt
      121 tacactgtca tatcctttgg caaagaatat cttctctgcg gcattaatga tatcctgacg
      181 tcttttttgt ttttctcttt cccttctgga agtggtagac atggtaactc acctgatgat
      241 ccctagacat tatatctgat gggactcctt aaaagattag ttttagtgga ttaaatagaa
      301 tctggtaact attgattttt taaatgaact tcaatggatc caatcctgga tcgataggac
      361 aaacacccct ccataaaaaa ctatatatga tcttaactac aagttaaaaa ttaaccaata
      421 gttaaaaatt aacttatggt taaaaaatat gtggaggtgc cagaggtggt ggaaaaaaaa
      481 tcagtagctg aaggatggcc gcaaatagta ggagactaca caattggcga tatagaaagt
      541 gccgtggcag tggtgacttt agggtcccat atggaagata taccagtcaa agcaggcgcc
      601 ggcatatccg gccctttaca caccgaaaat ttagggatag aaaaagtgat tgggaacgta
      661 gttgccaacc ctaacctcag attcttagtg gtgtgtggat ccgaagtaca aggacacatt
      721 accggtcaaa gcataaaagc actccacacc aacggtgttg acccagaaaa aggaaaaata
      781 atcgatgcct ccggagcaat acacttcatc gaaaacatgc cacctgaagc catacagaga
      841 ttccgtgacc aggtggaatt ggtggatatg gtggatattg aagactccaa tgatatccag
      901 tccagaatta aggactgcat cgtcaaagat tccggtgcat ttgaagagga aactttaata
      961 ctaaagttgc cgaaagaccc tgtggaacaa atagatcata aaaaaaatga aaacacctaa
     1021 gaccagaatc cttacagatt tccagtaact gctacatagg ctctggtcgg gttcctgatc
     1081 ttcagggacc cgacccgggc tcctccaaat taattcaacc actatatgta ttgctatcaa
     1141 ttaatttttt attttaagaa tgaacagttt ttttaaattc atatttttta gaccaaaaac
     1201 ccattattaa aattgaacat aataattaat tgggtggaaa cgttcttttt taccaatcct
     1261 aataaaattc tagaatcaga gccctgggca atatttggag ttatataccc aaagaatgcc
     1321 aacaaattcg aacgtggcag aataatgtga tggtgataac atggttgaga taaatgaaaa
     1381 agctccggtg ctggccaagg cccaaataga aattaaagcc acaccagaaa ctgtttggaa
     1441 aataatggct gatattgaag cttggcccaa atggaattct gatgtgaaaa aagcctgctt
     1501 aaagggagag ttaaaggaag gaacccaatt ccagtggaaa actaaggttg gtaaaatcac
     1561 ctccctactg cagaaagtgg aacctccaca ccttctggcc tggaccggga ggatcatggg
     1621 gataaatgcc atccacgtgt ggagaatcca aacaaaaaat aaaaaaaccc aagtaataac
     1681 tgaagaatcc tgggaggggg ctttaagtcg tgtcatgagt ggaaaaatgc agaaaatgct
     1741 ggaagagtct atcaactccg gtctgcagta tctgaaaact gaagcagaac ggttatcaga
     1801 ctcttaatcc aaaatatgat attattccgg ggatacatgg cataacccag ccaactgttt
     1861 gtggaagcta caaagatctt gcatcaaata gctgcaaaaa aaatggtgat tctggaactc
     1921 tgataggatc tctggctaca ctt
//
LOCUS       DAOQVW010000309         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_76027_length_1940_cov_3.361804, whole genome shotgun sequence.
ACCESSION   DAOQVW010000309 DAOQVW010000000
VERSION     DAOQVW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76027_length_1940_cov_3.361804"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKK85_06775"
     CDS             <1..422
                     /locus_tag="PKK85_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNS25754.1"
                     /translation="EDRIILAELVDSVADMTQETAYGICLSQVQFPVQFEEDYQEMVD
                     AVCETIRILKESLEMLDEDLAQAIAKAHEVEVNEEAVDRIERRIIKKLYNAYRKGDFG
                     ILKLIELKNIATRLGEIADRAEDASDRVLIMVAKRRG"
     gene            539..1063
                     /locus_tag="PKK85_06780"
     CDS             539..1063
                     /locus_tag="PKK85_06780"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5616 domain-containing protein"
                     /protein_id="HNS25755.1"
                     /translation="MARKVSSDQKARERRSKLLDFNGLEDQIILLDGYNVLITLESAL
                     FHPDTILESDDGVLRDTKAVFGKYRPHENTFHVLDILVTFLKKTGIKKVHFFYDRPVT
                     RSGELAKLTRAVLKKHSLPGDAATSPYVDRKLANSGDKVIATSDGPLMDKVKRVVDLP
                     GLIRSKNLIPSRKK"
     gene            complement(1103..>1940)
                     /locus_tag="PKK85_06785"
     CDS             complement(1103..>1940)
                     /locus_tag="PKK85_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HNS25756.1"
                     /translation="KLYGAGQDLLSDTFSGNIKGMGPGCAEMEFKERPSDPVVVFCCD
                     KTEPGAFNMPLFKMFADPFTTAGLVIDPSLHGGFEFEVFDVMDHKKVKMTCPDEMYDL
                     LALLGTTSRYVIKHIRRRSDNEIAASVSTERLNLMAGKYVGKDDPVAIVRSQSGFPAA
                     GEVVEPFAFPHLVGGWMRGSHNGPLMPVAQKNAYPVRFDGPPRVMALGFQIADCKLVG
                     PADMFDDPAYDRSRDLASEIAEYMRRHGPFEPHRLPADEMEYTTLPGVMEKLKDRMED
                     ME"
BASE COUNT          495 a          477 c          520 g          448 t
ORIGIN      
        1 gggaggaccg tatcatcctg gctgaattag tggacagtgt agccgacatg acccaggaaa
       61 ctgcctatgg catctgccta tcccaggtgc agtttccagt ccagttcgaa gaggattacc
      121 aggagatggt ggatgctgtg tgcgaaacca tccgtatact gaaggagagc ttggaaatgc
      181 ttgatgagga cctggcccaa gccatagcca aggcccatga agtagaggtc aatgaggagg
      241 ctgtggaccg cattgagagg aggattatca agaaactcta caatgcctat cgtaaagggg
      301 atttcggcat actcaaactc atcgagctta aaaacatcgc cacccgtttg ggtgaaatcg
      361 cagatcgggc cgaggatgct tcggaccgag tcctgatcat ggtggccaag aggaggggct
      421 agaattagag gcagccgaag ctgatctcag gtacctcctc aaccagggct acccccagag
      481 gggtgccctt aactttgtag ccgggcacta cctcctggat agggaccaga gaaactacct
      541 ggcccgtaag gtctcctcag accagaaagc tagggagaga aggtctaaac tccttgattt
      601 taacggccta gaagatcaga tcatcctatt ggatggatac aacgttctca tcaccctgga
      661 aagcgccctt tttcacccag acaccatctt agagagtgat gatggagtac tgagggatac
      721 caaggcggtt tttggcaagt accgacccca tgaaaatacg tttcatgtcc tggatattct
      781 ggtgactttt ttaaaaaaga ccgggataaa aaaggtgcac ttcttctacg atcgtcctgt
      841 gaccagaagt ggagaactgg caaagctgac ccgggctgtg ctcaaaaaac acagcctacc
      901 aggagatgca gctaccagtc cctacgtgga ccgaaaactg gcaaatagtg gggataaagt
      961 aattgccacc agtgacggtc ccctcatgga taaagtcaag agggtggtgg atctgcctgg
     1021 tttaatcaga tcaaaaaatc taattccatc cagaaaaaag tgagtaacta tttttcaaaa
     1081 aaaagaattc agggtatgga tcttactcca tatcctccat cctgtctttt aatttttcca
     1141 tcacacccgg caatgtggtg tattccatct catcggcagg tagtctgtgg ggttcgaagg
     1201 gtccgtggcg gcgcatgtac tcggcgattt ccgaggccag gtcccgtgaa cggtcatagg
     1261 cagggtcgtc aaacatgtct gcagggccca ccaatttaca gtcggctatc tggaatccca
     1321 gggccataac tcgtggcggt ccatcgaaac gtaccgggta agcgtttttc tgggctacag
     1381 gcatcagtgg gccgttgtga gagcccctca tccagccgcc aaccaggtgg gggaaggcga
     1441 agggttccac cacttcaccg gcagcaggga atccagactg ggatcttaca atagccacgg
     1501 ggtcgtcttt acccacgtac ttgccggcca ttaggttcag tcgctcggta cttactgaag
     1561 cagctatttc gttgtcactc ctcctccgga tatgcttgat gacgtagcgg ctggtggtgc
     1621 ccaggagagc caacaggtcg tacatttcgt cggggcaggt cattttaacc ttcttgtgat
     1681 ccatgacatc aaatacttcg aattcgaatc ctccgtgtag ggaggggtcg atgaccagtc
     1741 ccgcggtggt gaaggggtcg gcgaacattt taaacaatgg catgttaaag gcaccgggtt
     1801 cggttttgtc acagcagaac acgaccaccg gatcactggg tcgttccttg aattccatct
     1861 cggcacaacc aggccccatt cccttgatat tgcctgagaa tgtatctgaa aggaggtcct
     1921 gtccggctcc gtagagttta
//
LOCUS       DAOQVW010000310         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_76062_length_1940_cov_2.542175, whole genome shotgun sequence.
ACCESSION   DAOQVW010000310 DAOQVW010000000
VERSION     DAOQVW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76062_length_1940_cov_2.542175"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..861
                     /locus_tag="PKK85_06790"
     CDS             106..861
                     /locus_tag="PKK85_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS25757.1"
                     /translation="MILLGESVIKVNNVGMEFNLSQEKVDNLKEYVIKLLKRQLLYQE
                     FWALRDVSFEIEKGDRVGIVGVNGAGKSTLLKIISGVMKPTTGSLEITGRLVPLLELG
                     AGFDHNYTGRENIFLNGAILGYSKEFIEEKYEEIVEFSEIEQFMDVPLKNYSSGMIAR
                     LGFSIATVVEPEILVVDEVLSVGDARFKQKSQEKIMSLFNKGVTVLFVSHSVEDVKAI
                     CNKAIWLEKGKMVMQGEVNDVCDRYMESLGMNG"
     gene            864..>1940
                     /locus_tag="PKK85_06795"
     CDS             864..>1940
                     /locus_tag="PKK85_06795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS25758.1"
                     /translation="MSYKISVVIPVYNVERYIGDCLDSLVHQTLGIENIQVIVVNDCT
                     PDGSMAIVEEYSRKYPSIQVVEHEYNQGQGAARNTGLQYVKGEYITFVDSDDFISHNT
                     YEICLEKFEKYDCDLVIYEYVYFSESGKNYPRNPSGALFEDNQLIKDITMTPEIVFST
                     SLCNKVYPKKFKSILKFPNTIYEDMVPTITAIFRSQHIYITNECRYYYRKRETCIKST
                     TDDFLEKTDSYRDFIFIHHQLYTLLEEYPRYKSLIDWINSRDTRYFLLHLMQRRVFSH
                     HERQEIFYKAQDYMKEVHEDTLKRFDPFWREFLEDVQKKSYWPFLIKYRSIYPQVKHK
                     LLRVKHRTHLYYLRTLKVISISFFI"
BASE COUNT          600 a          387 c          418 g          535 t
ORIGIN      
        1 gatcctaaaa ccgtgctatt tgctactgca tcttcagtaa tactccttat tctggggata
       61 ttctttttct acaagtatca ggataaattc attctataca tctaggtgat attattgggc
      121 gaaagtgtta tcaaggtcaa caacgttgga atggaattta acttaagtca ggagaaggtg
      181 gataacctta aagagtacgt tataaaactc ttaaaaagac aactattata ccaggagttc
      241 tgggccctca gggatgtatc atttgaaatc gaaaaggggg atagggtggg aattgttggt
      301 gttaacgggg caggtaagag caccctcctt aagatcatat ccggggttat gaagccaacc
      361 acgggcagtt tagagatcac aggtagactg gtaccccttc tagaattagg ggctggtttt
      421 gatcataatt acaccggtag agaaaacata tttttaaatg gtgccatatt aggatattcc
      481 aaagaattta tcgaggaaaa gtacgaggag atagtggaat tctctgaaat cgaacagttt
      541 atggatgtac ccctgaaaaa ttattcctct ggtatgattg caagattagg gttcagcatt
      601 gcaaccgtgg tggaacccga gattctggtg gtggatgagg tactctcggt gggtgatgcc
      661 cgatttaagc agaaaagtca ggaaaagatc atgtcacttt tcaataaagg tgtaacggtt
      721 ctgtttgtat cccattccgt ggaggatgtt aaggccatct gcaataaagc catctggctt
      781 gagaagggaa aaatggtgat gcagggtgag gtaaacgatg tctgtgaccg gtacatggaa
      841 agtttgggaa tgaatgggta atcatgagtt acaaaataag cgttgtcatt ccagtttaca
      901 atgtggagag gtacattgga gattgtttgg attccctggt ccaccagacc ctgggcatag
      961 agaacataca agtcatagtg gtcaacgact gcactccgga tggtagtatg gccattgtag
     1021 aagagtattc ccggaaatat ccctccatcc aagtggtgga acatgaatac aaccagggac
     1081 aaggggcagc tcgtaacact ggtttacaat atgttaaggg agaatatatc accttcgttg
     1141 attctgatga ttttatttca cataacacct atgaaatctg tctggaaaag tttgaaaaat
     1201 atgattgtga cctggttatc tacgaatacg tctacttcag tgaatctggt aaaaattacc
     1261 cccgcaaccc ctctggagca ctcttcgaag ataatcagtt aataaaagac attacaatga
     1321 ccccggagat agttttctcc acctcccttt gtaacaaggt ctaccctaaa aagttcaaat
     1381 ccatcttgaa gttccccaac acaatctacg aggatatggt ccctactatc actgctattt
     1441 ttcgctccca acatatttac attaccaacg aatgtcgtta ctactaccgt aaacgggaaa
     1501 cctgtataaa atccaccacc gacgattttc tggaaaaaac cgacagttac agggatttca
     1561 ttttcatcca tcaccaactt tacactcttc tagaggaata tccccggtac aaatccctca
     1621 tcgactggat taactccagg gataccaggt actttctcct ccatctaatg cagcgaagag
     1681 tgttcagtca ccatgaaaga caggaaatat tctataaagc acaggattac atgaaagaag
     1741 tccatgaaga taccttgaaa cgcttcgacc ccttctggag ggagtttctg gaggatgtgc
     1801 agaaaaagtc ttactggccc ttcctcatca agtataggag catctatccc caggttaaac
     1861 acaaactatt gagggtaaaa caccgaaccc atctgtatta cttaagaaca ttaaaggtta
     1921 tctccatatc ctttttcatc
//
LOCUS       DAOQVW010000311         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_76389_length_1934_cov_15.058010, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000311 DAOQVW010000000
VERSION     DAOQVW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76389_length_1934_cov_15.058010"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="PKK85_06800"
     CDS             <1..1419
                     /locus_tag="PKK85_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25759.1"
                     /translation="SREFIEYLNNHNATVMDFLEFISTWLLSGESKNIMTVFFAHFST
                     ITGLRPIWTIFLGGPGEGKSAIEQAAFSLIPDRCKYSGRSSYAATLNQARDLGNDFLD
                     QKIISLGDLGGKNSYIKWEETLDVYKELTTEGFYDYKKMQDTPDPETRQKEVVTIRVE
                     GYPSVSFASTHSDGLTGQYLSRGITISPVGSDDDILRYRRFVRPATRAKAFRDELTGH
                     VMKLFHSYIETLLKDIETVEVINPYYLCLEDWFRGSNNIKRVSEMFPLLVDAVTLFNY
                     QRRHLITTVDGDKYYLATKEDNEVIAKLFDITPGLTAQVVTFYNKLVDRIGKYDYDEF
                     KDYQDGDLTIRDCKTIFTIPTLRNRRFKRASDDEKEQFSKWCQLLHDAGKLDRLMKNH
                     KGHNVYCLSDGEKMGTVNMNCDISKVKEYLEEDISGNGWGMGLDRDEIVSCIGDEKMH
                     RSSWTDFKTPPWDLNGEEEEVG"
     gene            1419..1775
                     /locus_tag="PKK85_06805"
     CDS             1419..1775
                     /locus_tag="PKK85_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25760.1"
                     /translation="MFWLNLCFPSFLSWGFLGTFQFKGPQFISKTLRVGVGRSPVYIL
                     KGVNFGGKDPNFSDSLILSWFKIIIRHTINKINITTSLNPGDLWYIKPYKGGDLPPSK
                     VPRKTGQKVEVGNFDL"
BASE COUNT          596 a          399 c          444 g          495 t
ORIGIN      
        1 agcagggaat tcatagaata cctaaacaat cacaatgcca ccgtgatgga cttcctcgaa
       61 ttcataagta cctggctact cagcggagaa agtaaaaata ttatgactgt ctttttcgcc
      121 cacttcagca ccatcactgg cttaagaccc atctggacca tcttcctagg tggaccgggt
      181 gagggaaaaa gtgccataga acaagctgcc ttcagtttaa tccctgacag gtgtaagtat
      241 agtggacgta gtagttatgc tgcgacactt aaccaggccc gtgaccttgg aaacgacttc
      301 ctagaccaga agatcatatc tcttggtgac ctgggaggaa aaaacagtta cataaaatgg
      361 gaggaaaccc tcgatgtcta caaggaatta accacagagg ggttctatga ttataagaag
      421 atgcaggaca cccccgaccc ggaaaccaga caaaaagaag tagtaactat acgggtggaa
      481 ggttatccaa gcgtatcctt cgcaagtaca catagtgatg gattaacagg acaataccta
      541 agccgtggaa tcaccatatc ccctgtgggg agtgatgatg atatactcag ataccgccgg
      601 tttgtccgcc ccgccaccag ggctaaggct ttcagggatg aactaaccgg ccacgtgatg
      661 aaactattcc acagctacat cgaaacatta ctaaaagata ttgagacggt ggaggttatc
      721 aacccctatt acttgtgcct ggaggactgg ttccgtgggt ctaataacat caaacgggtt
      781 agtgaaatgt tccccctctt agtagatgct gttacacttt tcaattacca acgccggcac
      841 cttatcacta ctgttgatgg ggataaatac tacctggcga ctaaggaaga caatgaggtc
      901 atagctaaat tatttgacat cacccccggc ttaactgccc aggtagtaac cttctataat
      961 aaactagtgg atcgtattgg taagtatgat tatgatgagt tcaaggatta tcaggacggt
     1021 gatttaacaa taagagattg taaaacgatt ttcacaatcc ccacactacg taacagacgg
     1081 tttaaacggg catctgatga tgagaaagaa cagttcagta aatggtgcca gctcctacat
     1141 gatgctggta aattggatcg gttgatgaag aaccataaag gccataacgt gtattgtttg
     1201 agtgatgggg agaagatggg tactgttaat atgaactgcg acatctcgaa ggttaaggag
     1261 tatttggagg aggatataag tggtaatggt tggggtatgg gattagatcg tgatgagatc
     1321 gtgtcttgta ttggtgatga gaaaatgcac cggagtagtt ggactgactt caaaacgcct
     1381 ccatgggatt tgaacggtga agaggaagag gtgggttaat gttttggtta aatctctgtt
     1441 ttcccagttt cctgtcttgg ggttttttgg ggacctttca attcaaaggt ccccagttta
     1501 taagtaaaac cctgagggtg ggggttggaa ggtccccagt ttatatacta aagggggtca
     1561 attttggggg aaaggacccc aacttttcag attctttaat actttcatgg tttaaaatta
     1621 ttatccgaca tacaattaat aaaataaata ttactacttc gttaaaccct ggggaccttt
     1681 ggtatataaa gccttataaa ggtggggacc ttcccccctc aaaggtcccc agaaaaacag
     1741 gccaaaaagt ggaggtgggg aactttgact tataaactgg atacctttaa atcaccagaa
     1801 aaaggggttt accaccatca acttcaccct gacctgaacc tattctatca ccaccagctg
     1861 catgaattca tgatccaata caccatcaaa acaataacca tgaccagcaa gccatggcca
     1921 cacaaccaac cact
//
LOCUS       DAOQVW010000312         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_77370_length_1919_cov_3.046137, whole genome shotgun sequence.
ACCESSION   DAOQVW010000312 DAOQVW010000000
VERSION     DAOQVW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77370_length_1919_cov_3.046137"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            443..1087
                     /locus_tag="PKK85_06810"
     CDS             443..1087
                     /locus_tag="PKK85_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25761.1"
                     /translation="MEYYQSRLKKFENNPLYANSYQSEKALYEAIANSTDLEEFGQKV
                     ESENLAVKNAIALVKDQETARKKFYEELKEEIKLHAPLRILDAVDSVKTDLELIKMVN
                     DIESEVNIEIIVDLFTQEIYNDLMMLEEIEVYQTAEVPDEWKKEINHERPQEMINDGI
                     KDWNESFVPNARNWDPNWQYNASLIWEERHRRIIPIPDEILKKRITQFKTYRGI"
     gene            1089..>1919
                     /locus_tag="PKK85_06815"
     CDS             1089..>1919
                     /locus_tag="PKK85_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25762.1"
                     /translation="MPVDKAMADSILGTFRKMFQELEDKGAEGESFQVMRETMDRMEK
                     LALETNDVSEFTAKLTTENLFMDFSNAYTETMTALVKGEYSGDGGDELLMEKTIEAYE
                     HAIENLKGNPHYEKLKAPLEELIEVGKSGVSYPVFLRIAEEQGLNQTLQGDMVVRDAI
                     LSEKLFCEFMHLPLEVEKHEKILKMHDQLASQTPFNVVDSFQFGLERQKIEWKYTPLI
                     NRWHIISRLWEKMIENVYDWLDPFGSFAPRDERWMSRRGITYTMHNIKRTQECNPGIL
                     K"
BASE COUNT          691 a          325 c          387 g          516 t
ORIGIN      
        1 cactccttcc acttataaaa acgattttct tcattcaact cattcagcat accatcctat
       61 atggcatacc tttcccatca cagggacaat tttcatttta gggtaagatt ggaaagtata
      121 taaatgtttc cattaaattt gaaaaaagat cacattaact tgaattgagt gtatcatttt
      181 acagtatttt tgagtcgaat acctcgtaaa actgtttatt taattaaatt ataagatgcc
      241 tgatttagat gattaagtca tggattatct ggttaataga ttagaaaatc attatcatac
      301 aatgctccca atcaagtagt aataaaaact caaggggata ataaaaaaac caatcattaa
      361 aactataaac ctatagtacc attgtaaatc tataattgat acaattagag ggatttaatt
      421 ggaccaaaga cagcaagaat atgtggaata ttaccagtcc agattaaaaa aattcgaaaa
      481 taatccgttg tatgccaatt cttaccagtc agaaaaagcc ttatatgagg ccatagctaa
      541 ttcaactgac cttgaagaat ttgggcagaa ggtggagtca gaaaatctcg cagttaaaaa
      601 tgccattgcc ctggtaaaag atcaggaaac tgcacggaaa aaattttatg aagagctgaa
      661 ggaagaaata aaacttcatg cacccctaag gattcttgat gccgtagatt ctgtaaaaac
      721 tgatttagaa ctaattaaaa tggttaatga tattgaaagt gaggttaata ttgagatcat
      781 tgtggatctg ttcactcaag aaatctacaa cgatcttatg atgctggaag aaatagaagt
      841 ttaccagacg gcagaagtgc ctgatgaatg gaaaaaggaa ataaatcatg aaagacctca
      901 ggaaatgatt aatgacggga ttaaagattg gaatgaatca tttgttccca atgctcggaa
      961 ttgggatcct aactggcaat acaacgctag cctcatctgg gaggaaaggc acagaagaat
     1021 aatcccaata ccagatgaaa tattgaaaaa gaggataact cagtttaaaa cttacagggg
     1081 gatttaagat gccagtggat aaggcaatgg ctgattcaat tctgggtaca tttcggaaaa
     1141 tgttccaaga gttggaagac aaaggggccg agggggaatc tttccaagtt atgcgtgaaa
     1201 ccatggatag gatggaaaaa ttggctcttg aaactaacga cgtatcggaa tttacagcaa
     1261 agctaaccac agaaaacctt ttcatggatt ttagtaatgc ttacacagag actatgactg
     1321 cccttgttaa aggtgaatat tctggagatg gtggtgatga actactcatg gaaaaaacca
     1381 tagaagctta tgaacatgcc attgaaaatt taaaaggcaa tccccattat gaaaaactca
     1441 aagctcccct tgaagaacta attgaagttg gaaagtcagg ggtatcctac ccagtattcc
     1501 tgagaatagc agaagaacaa ggtcttaatc agactttaca aggtgacatg gttgtgaggg
     1561 acgctatact ttctgaaaag ttgttttgtg agttcatgca cttgccattg gaagtggaaa
     1621 aacatgagaa gatcctaaaa atgcacgacc aactggcctc ccagacaccc tttaacgtgg
     1681 ttgacagctt ccaatttgga ctggaacgtc aaaaaattga atggaagtac actcccctga
     1741 ttaaccggtg gcacatcata tcacgtttat gggagaaaat gattgaaaat gtatatgact
     1801 ggctcgatcc tttcggcagt ttcgcgcccc gggatgaacg atggatgtct aggagaggca
     1861 tcacttatac tatgcataat attaaacgta cacaggaatg caacccaggc atccttaaa
//
LOCUS       DAOQVW010000313         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_77479_length_1917_cov_4.318475, whole genome shotgun sequence.
ACCESSION   DAOQVW010000313 DAOQVW010000000
VERSION     DAOQVW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77479_length_1917_cov_4.318475"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PKK85_06820"
     CDS             complement(<1..1061)
                     /locus_tag="PKK85_06820"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNS25763.1"
                     /translation="MLAGSEGLGGIYFDNPVITQAMGTIALVFILFSGGLDTKWGEVR
                     PVLGQGIVLSTLGVFITAVAVGLFINWVTNWPLLLSLLIGSIVSSTDAAAVFSIFCSH
                     RTGLKNNIEPLLELESGSNDPMAYFLTITLIHLILNPSTSLQSMILSLIQSIGLGILL
                     GVVWGKGSAKLLNSIKLQITGLYPVLLIALSGLMFTVTYFMGGNGFLSVYIGAMIIGN
                     SDFLHKKTQKEFFDAIAWLWQIFMFMILGLLVFPSEIIPVMGIGLLISFFLILVARPL
                     SIFLCLAPFQAGFKDKILISWVGIKGAVPIILATFPIVAGIQGADMIFNVVFFITITS
                     ALIQGSTIYTLARYLGLNQ"
     gene            complement(1285..>1917)
                     /locus_tag="PKK85_06825"
     CDS             complement(1285..>1917)
                     /locus_tag="PKK85_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNS25764.1"
                     /translation="YVVLELNPQIVRNEKKHGQPIYYGDAAQATVLEQLNIHEARIMV
                     VAISDPVGSRKIVDIAKKINPNVYIIVRTRYIYEIDELYSLGADEVIPEEFETSVEIF
                     SRVMKQYQLPPDKINQFIHEIRSDGYGMFRTLTNWEINTCSINDALTDLQVSSYTIEE
                     DKLLKELHLESQGLTPIAVARGKQTLRNLDDNFLLEPDDVLIYTHQAEVE"
BASE COUNT          551 a          414 c          453 g          499 t
ORIGIN      
        1 cactggttca agcccagata ccgggccagg gtgtaaatgg tggatccctg gatcagggcg
       61 gaggtaatgg tgatgaagaa aactacgttg aagatcatat cagccccttg aataccagcc
      121 actatgggga aggtggccag gataatgggg acagcaccct ttatacccac ccaggatatt
      181 aaaattttat ctttaaaccc tgcctggaat ggggccaggc ataaaaagat gctgaggggc
      241 cgggccacca gtattaggaa aaatgatatg agaagtccaa tacccattac tggaatgatt
      301 tctgagggaa acaccagaag acccaggatc atgaacatga aaatttgcca caaccaggct
      361 atcgcatcaa aaaactcttt ttgagttttt ttatggagaa aatcactatt tccaatgatc
      421 atggccccaa tgtagacgct tagaaagcca ttcccaccca tgaagtaggt taccgtaaac
      481 atcaggccag atagtgcaat caagaggaca ggataaagcc cggtgatctg tagtttgatg
      541 ctgtttaaga gttttgctga tcctttcccc cagaccacac ccagcaatat tcccagtcca
      601 atggactgaa ttagtgacag gatcatggac tgcagggatg tgcttggatt taagataaga
      661 tgtattaggg tgatggtgag gaagtaagcc atagggtcgt tgcttccgct ttctaattca
      721 agtaggggtt caatgttatt tttaagacca gttctatggg aacagaaaat ggagaacaca
      781 gcggctgcat cggtggagga aacaatggat ccgatgagta aggacagtaa caggggccag
      841 ttggtgaccc agtttatgaa cagccccacg gcaactgctg taatgaaaac tcccagggtg
      901 gatagcacaa tcccctgccc caaaactggt ctgacctccc cccacttggt atctaacccc
      961 ccggaaaaaa ggatgaacac tagtgctatt gttcccatgg cttgtgtaat cactgggtta
     1021 tcaaaataaa taccgccaag tccttcagaa ccagcaagca tgcctattaa aaggaaaaag
     1081 agtagggatg ggattcccag tcggtaggaa atttgctgat tatgatgctg ataaaaatca
     1141 acaaggatgc aaccaggagg aactgttcaa aaaccattac ctaaaatttc ttcgccaccc
     1201 actattaata tatattctgt gataaaattt ttggatatgc aattcaggat gcttgggagg
     1261 atactggaga caatttatga taaactactc tacctcggcc tgatgggtgt agatcaacac
     1321 atcatctggt tccagcagaa aattgtcatc caggtttcgg agggtctgct taccccgggc
     1381 cacggcgatg ggtgtcaagc cctgactttc cagatggagc tcttttaaca gtttatcctc
     1441 ttcaatagtg taggatgaga cttgcagatc agttaaagca tcgttgatac tgcaggtatt
     1501 gatttcccag ttggtgaggg ttcggaacat tccgtagcca tcggatcgga tttcatggat
     1561 gaactggttg attttatcag ggggtaactg gtactgcttc ataacccggc tgaagatctc
     1621 caccgaagtc tcgaattcct cgggtatcac ttcatccgcc cctaaggagt acagttcatc
     1681 gatttcataa atgtaacggg ttctgacgat tatgtagacg ttaggattaa tttttttggc
     1741 aatatccact attttacgac ttcccaccgg gtcagagatg gccaccacca tgatccgggc
     1801 ttcatggatg ttcagctgct ccagaactgt agcctgagca gcgtctccat agtagattgg
     1861 ttggccatgt tttttttcat ttctaacaat ttggggattc agttccaaca ccacgta
//
LOCUS       DAOQVW010000314         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_77635_length_1915_cov_3.274194, whole genome shotgun sequence.
ACCESSION   DAOQVW010000314 DAOQVW010000000
VERSION     DAOQVW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77635_length_1915_cov_3.274194"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1129)
                     /gene="cbiD"
                     /locus_tag="PKK85_06830"
     CDS             complement(50..1129)
                     /gene="cbiD"
                     /locus_tag="PKK85_06830"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HNS25765.1"
                     /translation="MVMEREGQGQYGITTGTAAAAAAVAALLSLERVVDSVPIKTPLG
                     KLDVLVEHSKKLGKDHGWATVRKMPYPDSDVTQNLEIQADVQLTNDPDILIKGGDGVG
                     TVTKPGLQVSLGEKAINPVPRTMILSNLQKILPEGKGLEVTISIPQGRKVAQKTMNPR
                     LGIINGLSILGTTGIARPMSQKSFQESLKCQLDVALAEGYEELIFVPGNIGEKLALNI
                     LSVDKDQIIHMGNHVGFMLQEAHDRGLNHLILLGHAGKLVKLAAGIFNTQHKLADGRR
                     EVITAHAGLMGASKPVLELIFNCNTTEEMISILRREGLVQEVFNSIATSIHNRILEVY
                     MIRTEILIMEMDGTILNSNHQLLIG"
     gene            complement(1129..1386)
                     /locus_tag="PKK85_06835"
     CDS             complement(1129..1386)
                     /locus_tag="PKK85_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0307 family thioredoxin"
                     /protein_id="HNS25766.1"
                     /translation="MVVKVEVFTSPSCPYCPMAVQVVDEVKKEMPNDLEIEKIDIMVD
                     REKAIEYGLMAVPAIALNGVVRFVGAPNKDELLSAIKEELQ"
     gene            complement(1415..>1915)
                     /locus_tag="PKK85_06840"
     CDS             complement(1415..>1915)
                     /locus_tag="PKK85_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNS25767.1"
                     /translation="SSIVGSIGVILSLTDLSELYQKIGVDMYSIKAGEYKDMGASYRD
                     LTAAEEKMLQEMVDQDYDYFIHLVAENRNLNESYVRSIAEGKIYTGSQAKDLKLVDET
                     GGKKQALDLAARLGGLSDSYRVITITPSQNFEDVINSLSTNAAYALGKGIGSFMEKGA
                     VEYNIR"
BASE COUNT          460 a          484 c          394 g          577 t
ORIGIN      
        1 ggagggaact ggcccagcaa acagattttc atattcccat cacccttaat cagccaatta
       61 gtaactgatg attgctgttt aagatggttc catccatttc catgattaaa atctccgtcc
      121 tgatcatgta cacttccaag atacggttat ggatggatgt ggcgatggag ttgaacacct
      181 cctgcaccag gccttccctc cggaggatgc tgatcatctc ttcggtggtg ttacagttaa
      241 aaataagttc cagtactggt ttagaagcac ccatcaaccc ggcgtgggca gttatcactt
      301 ctctccgccc gtcagccaat ttgtgctggg tgttaaaaat gccagctgcc agcttcacca
      361 gtttacctgc atgtcccagg agaattaaat gatttaaacc acgatcatgt gcttcctgga
      421 gcataaaacc aacatgattc cccatatgaa ttatctggtc cttatcaaca cttagaatat
      481 tcaatgccag tttttctcca atgtttcccg gcacaaagat aagttcttca tatccctcgg
      541 ccaaggccac atccaattgg cacttaaggg actcctggaa acttttctga gacataggac
      601 gggcaatgcc tgtggtcccc agtatggaca ggccgttaat aattcccaac cggggattca
      661 tggttttttg agccactttt ctgccctggg ggatgctaat ggtaacttct aatcccttac
      721 cttctggtag gatcttttgc aagttactaa ggatcatggt ccggggaaca gggttaatgg
      781 ctttttcccc cagggacacc tgtaagccgg gcttggtaac ggtacccact ccgtccccac
      841 ccttgattaa tatgtccgga tcatttgtta actgcacatc agcttgtatt tccagatttt
      901 gggtcacatc tgagtcagga tagggcatct ttcttactgt ggcccagcca tggtcttttc
      961 ccagtttttt cgaatgttca accagcacat ccaactttcc caggggagtt ttaatgggga
     1021 cagaatccac aactcgttcc agagaaagca gggcagccac tgcagcggct gctgctgcgg
     1081 ttcccgtggt gattccgtac tgtccctggc cttctctttc catgaccatt actgtaattc
     1141 ttctttaatg gctgataata gttcatcttt gttgggcgcg cctacgaagc gcaccacacc
     1201 atttaaggct atggcgggca cagccatgag tccgtactca atggcctttt cccggtcaac
     1261 cattatatca attttttcaa tttctaaatc atttggcatt tcttttttca cttcatccac
     1321 cacttgtact gccatggggc agtaagggca ggagggggat gtgaagactt caactttgac
     1381 aaccattttt tcacctctta catgagtata atcactatct tatattatat tcaactgcgc
     1441 ctttctccat gaaacttcca attccttttc ccagggcgta agctgcattg gtggagagac
     1501 tgtttataac atcctcaaag ttttgtgagg gggtgatggt aatcacccgg tatgaatcag
     1561 ataagccgcc cagtcgagct gccagatcca gggcctgttt tttcccacca gtttcatcca
     1621 ccagttttaa atctttggct tggcttccgg tatatatctt tccttcagct atgcttcgca
     1681 cgtaactttc attaagattt ctattctcag caacaaggtg aatgaaatag tcgtagtcct
     1741 gatccaccat ttcctgtaac atcttctctt cggctgctgt gagatcacgg taactagcac
     1801 ccatatcctt gtattcacca gccttaatgg agtacatgtc caccccaatc ttttggtaga
     1861 gttctgaaag atcagttaag ctcaggataa cacctatgct acctacgatg gatga
//
LOCUS       DAOQVW010000315         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_78007_length_1910_cov_3.227493, whole genome shotgun sequence.
ACCESSION   DAOQVW010000315 DAOQVW010000000
VERSION     DAOQVW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78007_length_1910_cov_3.227493"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1103)
                     /locus_tag="PKK85_06845"
     CDS             complement(99..1103)
                     /locus_tag="PKK85_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS25768.1"
                     /translation="MMVDSLKTYYEVSLNKRESKCKIASQTSATNSETLKSLWNEHQK
                     VLKRMKNGNSSPEACLDFSFMDLKIKIARILFTECVFCPWKCRVDRRFEVGVCGVTKS
                     GVASEFLHLGEEAPLIPSHTIFFAGCNLNCVYCQNWDISQNPQLGMYIRPSNLARRID
                     QRRRDGSRNVNFVGGDPTPNLHYVLKTMHLTTMNVPVVWNSNMYLSWESMQLLEGFVD
                     LYLTDFKYGNDECARKLSGISNYMKVVGTNHKWANKAGDMIIRHLVLPDHGVCCSLPL
                     MHWIYENLGEEVVINIMGQYQPQYHAFRYNDISRPTSSLEVEKVVDYAKDLGFINIIR
                     "
     gene            complement(1121..>1910)
                     /locus_tag="PKK85_06850"
     CDS             complement(1121..>1910)
                     /locus_tag="PKK85_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNS25769.1"
                     /translation="AQDGEGVSTAHESHSSCQMDLDPEKIAKKACRIALDSRGGGPVE
                     TGNMMVVLDHQAAAGLLSTFTQAINGDNVQRGRSIYAGKEGEQVAAKELSIYDDGTIK
                     GGLYSACGDGEGTPSQKTTIIKDGILQHFIYDIYTAQKGNIESTGNGMRASYADMPSV
                     NLSNLEIEFENYHDLSDVRGGFMVTDVLGAHTANPISGDFSVEVMNAFLIINGEIKRP
                     VKKAMISGNIFEILQGLKGLSGEKRQLGPFIIPRLVCSSLRVVG"
BASE COUNT          512 a          466 c          366 g          566 t
ORIGIN      
        1 attccattcc acagtgatta cagtaaatat cttctggctt gatttctcca ccacagttaa
       61 ggcatctcat ttagctacga ctccctttac tccctatgtt atcttatgat atttataaag
      121 cctaggtctt tggcataatc caccaccttc tcaacttcta atgaagaagt tggtcgggat
      181 atatcattat agcgaaaggc atggtattgg ggctggtact ggcccatgat attaatgacc
      241 acctcttcac ctaaattttc gtatatccag tgcatcagtg gtaacgaaca acacaccccg
      301 tgatcaggaa gaaccaggtg ccggattatc atatcccctg ccttgttggc ccatttatgg
      361 ttggttccca ccaccttcat gtaattggaa atacctgaaa gttttcgggc acattcatca
      421 ttcccatatt tgaaatccgt caagtacaag tctacaaagc cttccaaaag ttgcatggac
      481 tcccaggaga gatacatgtt gctattccaa accacaggaa cattcatggt agttaagtgc
      541 atggtcttta aaacgtagtg caggttggga gttggatcgc ctcccacaaa attcacattt
      601 cttgaaccat cccttcggcg ctgatcgatt ctcctagcca aattagacgg acgaatatac
      661 atccctaatt gtggattttg acttatatcc cagttctgac agtaaacaca atttaaattg
      721 caaccagcaa aaaatattgt gtgactggga atcaaaggag cttcctctcc aaggtgcagg
      781 aattcagagg ctacacctga ctttgtcact ccacagaccc caacttcaaa tctccgatca
      841 acccggcact tccaggggca gaacacgcat tcagtgaata atatcctggc aattttgatt
      901 ttaagatcca tgaaagaaaa atccaaacag gcctcaggag aagaattacc atttttcatt
      961 cgctttaaaa ctttttgatg ctcgttccat aaacttttta gagtttctga attggtggcc
     1021 gaagtctggg aggcaatttt acattttgat tctcttttgt tcagactaac ttcataatat
     1081 gttttaaggg aatcaaccat catagtaacc tcttattggg ctaccctacc acccgtaggc
     1141 tggaacatac taaccgggga atgatgaagg ggccaagttg ccttttttcg ccagataatc
     1201 cttttaggcc ctgtaagatt tcaaatatat tacccgaaat catagccttc ttcacgggtc
     1261 tttttatctc cccattaata attagaaaag cattcatgac ctccaccgaa aaatcgccgg
     1321 atatggggtt ggcagtgtgt gctcccagta catctgtaac cataaaaccc cctctaacat
     1381 cagataggtc atggtagttt tcgaattcaa tctccaagtt gcttagattg actgaaggca
     1441 tatcagcata ggaagccctc atcccattac cagtactctc tatatttcct ttttgggccg
     1501 tgtagatatc gtagataaaa tgttggagaa ttccatcctt gattatggtt gttttctggc
     1561 taggtgttcc ctctccatct ccacaggcag aataaagtcc tcccttaatt gtaccatcat
     1621 cgtagatgga gagttccttt gcagccacct gttcaccctc cttccctgcg tagatggaac
     1681 gtcctctttg aacgttgtca ccgttgatgg cttgggtgaa ggtggaaaga agtccagcag
     1741 cggcttgatg atccagcacc accatcatgt ttccagtttc cactggtccg ccacccctgg
     1801 aatccaacgc tatcctgcaa gccttttttg caattttttc tggatccaga tccatctggc
     1861 aggaagagtg ggactcgtgt gctgtggaaa ccccttcccc atcctgggca
//
LOCUS       DAOQVW010000316         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_78131_length_1908_cov_4.182947, whole genome shotgun sequence.
ACCESSION   DAOQVW010000316 DAOQVW010000000
VERSION     DAOQVW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78131_length_1908_cov_4.182947"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PKK85_06855"
     CDS             <1..677
                     /locus_tag="PKK85_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNS25770.1"
                     /translation="EKKVGDSINLFGESFTITGIFETGNIMYDGAAFTSLDTLQNITN
                     SQGEVSTIYVQVYDDTNLTELSQNIEATYPNELTASSASALSDRINEGLGIIDSATWA
                     ISLLAIFIGGVGVVNTMIMSIYERTREIGVLKAVGWRSRRILGMIMGESIVLTLIAAV
                     VGTVLGILAAEAGLSLLSGKISPAYSLDVFVRGFLVAILVGVLGGIYPAYRASRLPPT
                     EALRYE"
     gene            670..1362
                     /locus_tag="PKK85_06860"
     CDS             670..1362
                     /locus_tag="PKK85_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS25771.1"
                     /translation="MNKPIIQIKDLKKRYDHGKITALNGLNLNIKRGEFVSIMGPSGS
                     GKSTLLNMIGALDTPDSGQIMVNGIDLTVKKDLSQFRSQEIGFVFQMHNLIPNLNVLE
                     NVKIPMLEGNLNSKQMENRVMGLLKAVGLEDKIRQTPNKLSGGERQRVAIARALVNHP
                     SIILADEPTGSLDSKTGDIILKLLRNLHEQENVTLVMVTHEPYVAKMADRIVYVLDGR
                     VSKKGEQMLGPV"
     gene            complement(1425..>1908)
                     /locus_tag="PKK85_06865"
     CDS             complement(1425..>1908)
                     /locus_tag="PKK85_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNS25772.1"
                     /translation="VAHLAELELASGLGDVMGEAHGGIVLRLKEGAPGWGMADRILLD
                     EELYVISRTLGEIDTADIINNPQHQARINQAGSELLGLLLNNPTPTQFMHLSRRFIHE
                     AQLMDEELEEILKIMDEESLGASMAMLGQTAFAISSEPYSSVEKTLVSRIDNWGCRFL
                     "
BASE COUNT          552 a          420 c          430 g          506 t
ORIGIN      
        1 tagaaaaaaa ggtgggagat agcattaatc tattcggaga atcttttacc attaccggta
       61 tatttgaaac cggtaacata atgtacgatg gggcggcttt cacctcctta gataccctac
      121 agaacataac caacagtcaa ggagaagttt caaccatata tgtgcaagtt tatgatgata
      181 ccaacctaac ggaacttagt caaaatattg aagccaccta ccccaatgaa ctaactgcct
      241 cctcagccag cgccctcagc gacagaatca acgaaggact cggcatcatt gactcggcaa
      301 cttgggccat ttcattacta gcaatattta tcggaggagt tggagtggta aataccatga
      361 ttatgtcaat atatgaacgt accagagaaa taggggtcct gaaagcagtg ggttggagga
      421 gtaggagaat tttgggcatg atcatggggg aatcaattgt tctaaccctt attgcagctg
      481 tagtgggtac tgtccttggt atcctggcag ctgaagccgg attgagtcta ttgagtggaa
      541 aaataagccc tgcttactct ttagatgtgt tcgtaagggg ttttttagtg gcaatcctag
      601 tgggtgtgct gggtggaatt tacccagcct accgggcttc ccggctccca ccaaccgagg
      661 cactgcgtta tgaataaacc tataatccag atcaaggatc tgaaaaaaag gtatgatcac
      721 gggaaaatca ccgccctgaa tggcttgaac cttaacatta aaaggggaga atttgtatcc
      781 ataatgggcc cttccggctc tgggaaatct acgctcctga acatgatcgg agcattggat
      841 acccctgaca gcggccaaat tatggtgaat ggtattgatc tcacagttaa aaaggatttg
      901 agccagttca gatcccaaga gataggcttt gtattccaga tgcacaatct catccccaac
      961 cttaacgtcc tggaaaacgt gaaaatcccc atgttggaag gtaatctgaa ctccaagcaa
     1021 atggagaata gagttatggg gcttttaaaa gctgtgggat tggaagacaa gatccgtcaa
     1081 acaccgaaca aactatccgg tggagagcgg cagagagtag ctattgctcg agccctggtg
     1141 aaccatccat caattattct agctgatgaa cccaccggat ccctggattc caagactgga
     1201 gatattattt tgaagttact gcgcaatctt catgaacagg aaaacgtaac ccttgttatg
     1261 gtaacccacg agccttacgt ggcgaagatg gcagaccgga tcgtgtacgt cctggatggg
     1321 agagtgtcaa aaaaaggaga gcaaatgtta ggaccagtgt gaatcagttt cactaattca
     1381 ttttcttcta gttattaccc agaaattgct ttatcaaaat tttattataa aaaccggcac
     1441 ccccagttat ctattctact cactaaagtt ttttcaacac tggaatatgg ttctgatgat
     1501 attgcaaatg cagtttggcc taacatggcc atggaggcac ctaggctctc ctcatccatt
     1561 attttgagta tttcttcaag ctcctcatcc attagttgag cttcatggat aaatctccgg
     1621 gatagatgca tgaactgggt gggagtggga ttatttaaaa gtaaacccag aagttctgat
     1681 ccagcttgat ttattctagc ctgatgttgg gggttgttga tgatatcagc agtatcaatc
     1741 tcccccaggg ttcgggatat cacgtagagc tcctcatcta acaagatgcg atcagccatg
     1801 ccccacccgg gagctccctc tttcaatctt aaaactatcc ccccatgtgc ttcgcccatg
     1861 acatctccca atccactggc aagttctaac tctgcgagat gagccact
//
LOCUS       DAOQVW010000317         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_78418_length_1904_cov_3.797188, whole genome shotgun sequence.
ACCESSION   DAOQVW010000317 DAOQVW010000000
VERSION     DAOQVW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78418_length_1904_cov_3.797188"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PKK85_06870"
     CDS             <1..413
                     /locus_tag="PKK85_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNS25773.1"
                     /translation="IQELKVGEQVIIGTDRGTYSVTTSPNPNSTSSAFIGIKSQEKHV
                     VSEDLSIILGDAPWVLVYLAELFMWIFTLNFGIGLFNLLPLKPFDGGLIFEEIIGNWT
                     SERVTRACTYAVSIFCLSLVLINIGYGFLTAIFY"
     gene            complement(438..602)
                     /locus_tag="PKK85_06875"
     CDS             complement(438..602)
                     /locus_tag="PKK85_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25774.1"
                     /translation="MKKKRIRSHTPVTCQCQPPAVERPPSILRSVQCPDCGKFFRTNR
                     QVDHCFKCRP"
     gene            complement(620..943)
                     /gene="tusB"
                     /locus_tag="PKK85_06880"
     CDS             complement(620..943)
                     /gene="tusB"
                     /locus_tag="PKK85_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HNS25775.1"
                     /translation="MRIGFIVTKTPAEYGFKTFLQFIKIYLGKKDVYVYLVGDGVYCA
                     RKGHQKSDEIIRIIENGRLYALLEDLQARAITPERTFPGTKILDSYQDLVVAIMEEMD
                     QMLSF"
     gene            complement(968..1585)
                     /locus_tag="PKK85_06885"
     CDS             complement(968..1585)
                     /locus_tag="PKK85_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNS25776.1"
                     /translation="MTDEQNRHLILADGALVKEVSIKEIRPCIVAEGMVRVLMQLDNS
                     LDAVIPSLMTIYPPGKVNYIKNKNILTLSLHNRLITIYPSGKVSMNKTLDEEDALETI
                     TKIMVDINQAHLQESTTGTSDLQDAQERLSNLGPLDLYHCLPQTGCGECGEATCTAFA
                     MKLLSGDTTLDCCTPLKESEHKEKLRCLENLLGEPLMAALGWKIQ"
     gene            complement(1595..>1904)
                     /locus_tag="PKK85_06890"
     CDS             complement(1595..>1904)
                     /locus_tag="PKK85_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNS25777.1"
                     /translation="FSAYYVAIACLNREFDADVFLVGDGVYSALENQKSQETLKFPNV
                     GELAYLVFPEGSIFVHKDSMEKRGIFGEELVESAQIIDDEELFKILESKMGKIAFINF
                     "
BASE COUNT          495 a          430 c          400 g          579 t
ORIGIN      
        1 tgatccaaga acttaaggta ggagaacagg ttatcatagg cacagataga ggcacttaca
       61 gtgttactac cagtcccaat cccaacagta ccagtagtgc cttcatcggg attaaaagcc
      121 aggaaaagca tgtggtcagt gaagatctgt ccataatcct gggtgatgct ccctgggtgc
      181 tggtgtatct ggccgaactt ttcatgtgga tattcaccct taattttggt attggactgt
      241 tcaacctatt gcccctaaaa ccctttgacg gagggctgat ctttgaagag atcataggta
      301 actggacctc tgaaagagtt accagggcat gcacctacgc agtgtcaatc ttttgcctaa
      361 gcttggtcct gataaatata ggttacggat ttttaaccgc aattttttat taaaaacagg
      421 cctttatccg gcgaaaacta gggacgacat ttaaaacaat ggtcaacttg gcggttggtc
      481 cggaaaaact tcccacaatc aggacactgc actgaacgca ggatagatgg gggtctctcc
      541 acggcagggg gctggcactg gcaagtaacc ggtgtatgac ttctaattct ttttttcttc
      601 atttttcccc ctattttttc taaaaactca acatctggtc catttcttcc attattgcca
      661 ccaccagatc ctggtagctg tccaggattt ttgtgccagg gaaggtacgt tccggggtta
      721 tggcccgggc ctgaagatcc tctaaaaggg cgtaaagccg accattttcg atgatcctga
      781 tgatttcatc ggacttttga tgacctttac gtgcacagta cactccatct ccaacaagat
      841 acacataaac atcttttttc cccagatata tttttataaa ctgtaaaaat gttttaaacc
      901 catattcagc cggggttttg gttacaataa atcctatgcg catttacacc atcctttgca
      961 gttcatttta ttggatcttc catcccaggg cagccataag aggttccccc agtaagtttt
     1021 ccaggcatct cagtttttct ttatgttctg attcttttaa aggagtgcag cagtcaaggg
     1081 tggtgtctcc agagaggagt ttcatggcaa aagcggtgca ggtagcctct ccacattctc
     1141 cacagccagt ctggggtaaa cagtggtaga ggtccagggg tcctaggttc gaaagtcttt
     1201 cctgggcatc ctgcagatca gaagtaccgg tggtggactc ctggagatgg gcctggttta
     1261 tatcaaccat gatcttggtt atggtctcca gggcatcctc ttcatctagt gttttgttca
     1321 tactgacctt tccagaagga taaatggtta taagtcgatt gtggagtgat agggtgagga
     1381 tgtttttatt ttttatgtaa ttaacctttc ccggaggata aatggtcatt aatgaaggaa
     1441 ttactgcgtc tagggaatta tccagttgca ttagtactct aaccattcct tcggccacta
     1501 tgcaggggcg gatctccttg atgctaacct cttttaccag tgcaccatca gcaaggatca
     1561 ggtgcctatt ttgctcatca gtcattcttc acctctaaaa gttgataaag gctatttttc
     1621 ccattttcga ctccaaaatc ttaaataact cctcatcatc aataatttgt gctgattcaa
     1681 ccaattcttc cccgaatatc cctctttttt ccattgaatc cttatgaaca aatattgatc
     1741 cttccgggaa tactaggtag gctaattccc ccacgttggg gaatttgagg gtttcctgtg
     1801 atttttgatt ttccagagca gaatacactc catccccaac tagaaataca tcagcatcaa
     1861 attcacggtt cagacatgca atagccacat agtaagcact gaat
//
LOCUS       DAOQVW010000318         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_78521_length_1903_cov_2.664502, whole genome shotgun sequence.
ACCESSION   DAOQVW010000318 DAOQVW010000000
VERSION     DAOQVW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78521_length_1903_cov_2.664502"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1903
                     /locus_tag="PKK85_06895"
     CDS             <1..>1903
                     /locus_tag="PKK85_06895"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS25778.1"
                     /translation="IIEYNFVGGKATTVDPKTGAAILGYTPQEGNIDYMTRRVASWVK
                     LKYLPNADKLVSLIYYNYPPGKDNIGSSYMDTITSIYNLLLVLKAEGYTVENIPSNST
                     QLENLMITLGINVANWAPGELQKLANNPNIILYPVEDYQAWFNQLDPITRLQVVEGPV
                     AYIGELCRRAVQLDYTTGMVSRIDTWYSGVISLLPSDKASAALPILDNIVRSLKNYIS
                     THKPGDYQAYLNYKDQFRDLYIRGMNGWGEAPGDIMVVEKNGKKYFVIPGMKFGKIFI
                     GPEPQRGWEGDIAKLYHSSTVAPPHQYLAYFAYLQEQGTNAMVFMGRHATHEWLPGKE
                     LVLSPHDLPSVCVGRVPQVYYYIVDGLAEGIQAKRRGYAVIISHLTPPMTFTHLYGDL
                     GTLATLASDYDGATSTQKPEIITKIRSLIQKNHYSLGADASTLSDEELINAVEDYLAD
                     IQSTLYPYGLHAIGQAWSEDEISLLVTSILSVDFAVSTTETTTLQEEISQVMYGKSYD
                     NLAALDKDRVQAKCVEVVKALIYNEVDTVAAVLTTTPSPKLKAALEKAKYYITAIQES
                     VQNEVRSLLNALNGGYIVPGAGGDPVANPDVLPTGSNFFHDQAAEIPTQEAYTYAKVP
                     LGEWMKDAFG"
BASE COUNT          494 a          536 c          501 g          372 t
ORIGIN      
        1 ataattgaat acaacttcgt agggggtaag gccaccactg tggatccaaa aacaggggcc
       61 gcaattttag gctacacccc ccaggaggga aacatcgact acatgaccag aagggttgct
      121 agctgggtta aactcaagta tctgcccaac gccgataaac tggtatctct catatactac
      181 aactaccccc caggtaagga caacattggc tccagttaca tggacaccat taccagtatc
      241 tacaacctcc tactggtcct caaggcagag ggctacacag tggagaacat ccctagcaac
      301 agcacccaac tagagaattt gatgatcacc ctgggtatca acgtggccaa ctgggcccct
      361 ggggagctgc agaaactggc taacaacccc aacatcatac tctacccagt ggaggattac
      421 caggcctggt tcaaccagct ggatcccata accaggttac aagtggtgga aggaccggtg
      481 gcctacattg gagagctctg ccgaagggcg gtgcaactgg attacaccac ggggatggtc
      541 agcaggatag acacctggta ctccggggtc atctcactcc tccccagtga caaggccagt
      601 gcagccttac caatcctgga taacatcgtc aggagcctta aaaactacat cagcacccac
      661 aagccagggg actaccaggc ctacctcaac tacaaggacc agttccggga cctgtacatc
      721 cggggtatga atggctgggg agaggcccct ggtgacatta tggtggtgga gaaaaatggg
      781 aagaagtact tcgttattcc tggtatgaaa ttcggcaaga tcttcatagg cccggagccc
      841 cagcggggat gggaggggga tatagctaaa ctgtatcaca gcagtaccgt ggccccaccc
      901 caccagtacc tggcctactt cgcctacctc caggagcagg gcaccaatgc catggtgttc
      961 atgggccggc acgccaccca tgaatggctg ccgggtaagg aactggtact cagcccccat
     1021 gacctgccca gtgtatgtgt gggccgggtg ccccaggtct actactacat cgtggatgga
     1081 ttggctgagg gtatacaggc caagcgccgg ggctacgcag tgataatcag ccacctaaca
     1141 ccccctatga ccttcaccca cctctacggg gatctgggta ccctggctac cctggccagt
     1201 gactacgacg gtgccaccag cacccagaag cctgagatca tcaccaagat caggtccctc
     1261 atccaaaaga atcattacag tttgggtgcg gatgcctcca ccctgagtga cgaagaattg
     1321 atcaatgcag tggaggatta cctggctgat atacagagca ccctctaccc ttatggtttg
     1381 catgccatag gacaggcctg gagtgaagat gaaatcagcc tcctggtgac cagcatactc
     1441 tcagtggatt tcgcagtcag caccaccgaa accaccacct tacaggaaga gatctcccaa
     1501 gtaatgtatg ggaagtccta tgataacctg gccgccctgg ataaggacag ggtgcaggcg
     1561 aagtgtgtgg aggtggtgaa ggccctaatc tacaatgagg tggatactgt ggccgcagtt
     1621 ttaaccacca cccccagtcc taagctcaaa gctgccctgg agaaggctaa gtactacatt
     1681 actgccatcc aggagagtgt tcaaaatgag gtgcggagtc tcttaaatgc ccttaatggg
     1741 gggtacatcg tccccggggc tggtggcgac ccggttgcca atccagatgt gctccctact
     1801 gggagcaact tcttccatga ccaggccgca gagataccca cccaggaggc ctacacctac
     1861 gccaaggtcc cgctcggcga atggatgaag gacgcattcg gct
//
LOCUS       DAOQVW010000319         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_78871_length_1897_cov_4.152552, whole genome shotgun sequence.
ACCESSION   DAOQVW010000319 DAOQVW010000000
VERSION     DAOQVW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78871_length_1897_cov_4.152552"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..238)
                     /locus_tag="PKK85_06900"
     CDS             complement(62..238)
                     /locus_tag="PKK85_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25779.1"
                     /translation="MVKMELDERIEMENDHNLMVGPVKDKQIQDLEKRVQILEAQVKK
                     QGEWIDIIFRGDIV"
     gene            complement(257..580)
                     /locus_tag="PKK85_06905"
     CDS             complement(257..580)
                     /locus_tag="PKK85_06905"
                     /inference="COORDINATES: protein motif:HMM:NF022019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNS25780.1"
                     /translation="MDDDGDFIYFIVSEDGFVKIGKSRTNPYYRLSSLQTGNPHKLEL
                     KLAIKGHPIIEKLMHKLLQKYHYRGEWYRFEGVLKQFITYPHSMSLVIKDLYFVETKQ
                     MEWVF"
     gene            828..965
                     /locus_tag="PKK85_06910"
     CDS             828..965
                     /locus_tag="PKK85_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNS25781.1"
                     /translation="MEIMKVKACFDIEEEDLNQIDVIAKKEERSRSFILREAVRKYLE
                     K"
     gene            complement(1042..1188)
                     /locus_tag="PKK85_06915"
     CDS             complement(1042..1188)
                     /locus_tag="PKK85_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25782.1"
                     /translation="MDELVRLEVVFESNYQQLSQLDPGCVEYRRLKGENQRNLERRRE
                     LLGL"
     gene            complement(1537..>1897)
                     /locus_tag="PKK85_06920"
     CDS             complement(1537..>1897)
                     /locus_tag="PKK85_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25783.1"
                     /translation="LNTRCGWGTVGRQVYFRSHNLRKYFSNRLMSRESTMSFENVEYL
                     LNHQHWNSTQRAYYKPDVDELRQEYLENMHLLSITEDVQVRVVTTEDLEDLERMRTRM
                     DHLERYLKDLERHTKKY"
BASE COUNT          532 a          399 c          361 g          605 t
ORIGIN      
        1 ctatgcctac tattaggcac acttccccgg tgtttgcagg tgaggcaggc attactcata
       61 ttcatactat atcccccctg aatatgatgt caatccattc gccttgtttt ttaacctgtg
      121 cttctaatat ttggactcgt ttttcaaggt cttggatttg tttgtcttta acggggccga
      181 ccattaggtt gtggtcgttt tccatttcaa ttctttcatc cagttccatt ttcaccatct
      241 ctctcccatt gttttcctaa aaaacccatt ccatctgttt tgtttcaaca aaatacaaat
      301 ctttaatgac taacgacatg gaatgaggat aagtgataaa ctgctttaaa actccttcaa
      361 atcggtacca ttccccccta taatggtatt tttgcaatag tttgtgcatt aacttctcaa
      421 ttatgggatg ccctttaatt gcaagtttta actctaattt gtgggggttc ccagtttgaa
      481 gtgaacttaa cctataatat gggtttgttc tggatttccc tattttaaca aaaccatctt
      541 ctgaaacaat gaaataaata aaatccccat catcgtccaa tgaataataa ctgaatgcca
      601 tattgtgcct cgtttccctt tttcctgttt ccccccatcg tatggtggga aacgagggac
      661 atttaaaaaa aagtccctca tggcaagaag tggtccttct tgttgtactt atataagaaa
      721 gtaatagtat ataatatttt ctattatatc ctaatatttt agtatagaaa agtatatcta
      781 aggtgagaga cacaaatagt aaataataaa ataaaaggag ggataatatg gagatcatga
      841 aagtcaaagc ctgtttcgat atagaagagg aagaccttaa ccagatagat gtaatcgcta
      901 aaaaagaaga acgcagtcgt tcatttatcc tgcgtgaagc tgtacgtaaa tatttagaaa
      961 aataactacc tttaatatct ttaattactt ttattgcatt tttatgtccg ggccgggact
     1021 gggggggact ggccaggtca atcataaccc taatagttcc cttcgccgct ccaggttacg
     1081 ttgattctca ccctttaacc tgcgatactc aacacacccc ggatccaact gggataattg
     1141 ttgataatta ctttcaaaaa caacttccaa cctgacaagc tcatccatcc ccacaccccc
     1201 acacaatggc caactgtgtg gggagaataa ggagtggata gcccaagcta aggctaaagg
     1261 ttcctttagc agtctgtaaa cctttatacg gtggtgcaaa tataggtttc attggtacgt
     1321 ctcccgtaga tgtgccccaa cgtccttaac tggacagaga aggtggtgct tctgtgaagt
     1381 ccattaaggg ccttggttac aattaggact atttattttc atgatataaa taggtggata
     1441 gagagcgtct gggaaatact aaggaatcta ttttgtaata aaagaaggtt aatatactaa
     1501 aagaagtggt ggacaatttt ttcgagcccc ggttagtcaa tattttttgg tgtgtctttc
     1561 cagatccttg aggtatcgtt ccaggtggtc catcctggtc ctcatccgtt ccaagtcttc
     1621 taggtcctcc gtggttacta cccttacctg aacatcctcg gtgatactta gtaggtgcat
     1681 attctccaag tactcctgtc gaagctcatc cacatcaggt ttataataag cccgttgtgt
     1741 actattccag tgctggtggt tgaggaggta ttccacgttc tcaaaactca tggtgctttc
     1801 acggctcata agcctattag aaaagtattt gcggaggtta tggctcctga aataaacctg
     1861 ccgacctacg gttccccatc cacaccgggt gttgagt
//
LOCUS       DAOQVW010000320         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_79484_length_1888_cov_2.961266, whole genome shotgun sequence.
ACCESSION   DAOQVW010000320 DAOQVW010000000
VERSION     DAOQVW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79484_length_1888_cov_2.961266"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..907
                     /gene="comA"
                     /locus_tag="PKK85_06925"
     CDS             134..907
                     /gene="comA"
                     /locus_tag="PKK85_06925"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043817 - phosphosulfolactate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HNS25784.1"
                     /translation="MQAFNFLTHDRKPLPGTGITMMLDKGLGTRAVEDLLKISGSYID
                     LAKLGWGTSALYNKDIIMEKINLYRTYDVIPYPGGTLLETAYIQDKLDQFLLEAQQLG
                     FGAIEISDGTIELPIEDRIEIIGKVKDGGFKAITEVGKKDPQKDKEIKLQRKVEMINE
                     DIDSGADWIIMEAREGGKGIGVYDDKGNVKEEEVQFFMDNTKPEMIIWEAPQKNQQVY
                     FILNLGVNVNLGNIAPEEIISLETMRRGLRGDTLGKVNW"
     gene            907..1677
                     /locus_tag="PKK85_06930"
     CDS             907..1677
                     /locus_tag="PKK85_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS25785.1"
                     /translation="MIEKITIKGGYDKQGNKEPLNEVIIKKGEIFGVVGPTGSGKSSL
                     IGDIEQLAQEDTFSKRKILVNDEEPSYEDRTDPRKKMVAQLSQNMNFLADMTVGDFLS
                     LHAKCRGASNTCVDRVVKLANTLTGEPIKKEHELTILSGGQSRALMVADVAIISNSPI
                     VLIDEIENAGIRKHDALKVLAGHGKIVLVVTHDPVLALMTDKRIVMRNGGMIKVVGTT
                     QSEKGISRKLNKIDELMLNLREKVRNGEVIENIEDIEF"
     gene            complement(1682..>1888)
                     /locus_tag="PKK85_06935"
     CDS             complement(1682..>1888)
                     /locus_tag="PKK85_06935"
                     /inference="COORDINATES: protein motif:HMM:NF026203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNS25786.1"
                     /translation="ESVDAFDIFLARYTQEDHFYKYALYLQAKAHYFLKNYQTAVDLL
                     RRAIETDPDFEEAHDLLNSISNNI"
BASE COUNT          644 a          308 c          450 g          486 t
ORIGIN      
        1 ctactataaa tatgctaaaa aggtgggaga agattttgta ggaagatcca ccagtacttt
       61 cacatcccca tactctatat atataatagg aatgttttaa atatcaaaat catataaaaa
      121 aatggtgaaa tgattgcaag cttttaattt tctaacccat gatcgcaaac ccctacccgg
      181 cactggaatt accatgatgt tggataaggg tctgggcacc agagccgtgg aggatcttct
      241 gaaaatttcg ggaagttaca tcgatcttgc taaactagga tgggggacct ccgcacttta
      301 taacaaagat attataatgg agaaaattaa tttatacagg acatatgatg ttatacctta
      361 tcctggcggt actctgttgg aaacggcgta cattcaggat aagttggatc agttcctcct
      421 ggaagcacaa caacttggat ttggggccat tgagatctct gacggcacca ttgaactacc
      481 aattgaagat cggattgaaa tcataggtaa agttaaggat ggtggattta aagccataac
      541 tgaagtggga aaaaaggatc cgcaaaaaga taaggagata aagctccagc ggaaggtgga
      601 aatgatcaat gaagacatag attccggtgc ggattggatt attatggagg cccgcgaagg
      661 aggaaagggc atcggtgttt atgatgataa aggaaatgtt aaagaagaag aagtccaatt
      721 cttcatggat aataccaaac ccgagatgat tatctgggaa gcgccccaga aaaatcagca
      781 agtatatttt atactgaacc ttggggttaa tgtgaacctg ggcaacattg ccccagaaga
      841 gataatctca ctggaaacca tgcgtagagg tctcagggga gatactttgg gaaaggtgaa
      901 ttggtaatga ttgaaaagat aaccatcaaa ggcggttacg ataaacaagg gaataaagaa
      961 cctctgaatg aggtgatcat aaaaaaagga gaaatctttg gagtggttgg acctactgga
     1021 agtggtaaaa gttctcttat tggtgatatt gaacaactgg cccaggaaga caccttttcc
     1081 aagcggaaga tcctagttaa tgatgaggaa ccaagttacg aagacagaac cgatcctcgc
     1141 aagaagatgg ttgcccaact gtctcagaac atgaatttcc tggcagatat gactgtaggg
     1201 gactttttaa gtttacatgc taaatgcagg ggagccagta atacttgtgt ggatagagtg
     1261 gtgaaactgg ccaacaccct cactggtgag cccataaaaa aggaacatga acttacaatc
     1321 ctaagtgggg gccaatcaag ggcattgatg gtggcagatg tggctataat cagtaactcg
     1381 cctattgttc taatagatga gattgaaaac gctggtataa ggaaacacga tgctcttaaa
     1441 gttttggctg gccacggaaa aatagtgtta gtagtcaccc acgaccctgt tctggcgtta
     1501 atgactgaca agcgtattgt gatgagaaat ggtggaatga ttaaggtggt gggtaccacc
     1561 caatcggaaa aaggaatttc acgtaaacta aacaaaatcg acgaattgat gctcaattta
     1621 agggaaaaag ttcgaaacgg ggaagtaatc gaaaatattg aagatattga attttaatat
     1681 gttaaatatt attagaaata gaattaagaa gatcatgggc ttcttcaaaa tctggatctg
     1741 tttcaatggc cctgcgaagt aaatcaactg cagtttgata atttttaagg aaataatgtg
     1801 ccttagcttg taagtataag gcatacttgt agaagtggtc ttcctgtgta tatctggcca
     1861 gaaaaatgtc aaatgcatcc acagattc
//
LOCUS       DAOQVW010000321         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_79491_length_1888_cov_2.751227, whole genome shotgun sequence.
ACCESSION   DAOQVW010000321 DAOQVW010000000
VERSION     DAOQVW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79491_length_1888_cov_2.751227"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..678
                     /locus_tag="PKK85_06940"
     CDS             271..678
                     /locus_tag="PKK85_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNS25787.1"
                     /translation="MTNTQIQALAASIVSGKTSSYARAEAIFNWVRDKVGYSFYYNTK
                     NGAVKTLNTLAGNCVDTSNLLVALCRAVGIPARYQHGTCKFTSATYGHVWVQIWINGV
                     WYNADASSSRNFLGVVKSWTLSTLKGTYTSLPF"
     gene            700..1056
                     /locus_tag="PKK85_06945"
     CDS             700..1056
                     /locus_tag="PKK85_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNS25788.1"
                     /translation="MMIKKGIIAMVAIVAVVGLVYAAYDDPGKDASNDSEYMKHPDHD
                     NSSDDSTLISASLAKKLAERYIEEPGATPGTPQLLTSGKKRIYLVPVIYQGEKVGEIE
                     IDAETGKNLGGSGGAP"
     gene            1195..>1888
                     /locus_tag="PKK85_06950"
     CDS             1195..>1888
                     /locus_tag="PKK85_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029808.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNS25789.1"
                     /translation="MKILFVEPPKEFWFVMGEYLPPPLGILQLAAFLEEKGDDWDLEV
                     LDCQAEELKWSGLERRLESANPDLLVPSALATCNAYTILRTVSLAKKIDPDIKTVVGG
                     QHFTALAQESLEQYPEIDFIVRGEGELTLLELVSSLDREKSQLNIPGLSFRHKNKIVH
                     NPPRPLIKNLDDLPLPGYHFIEDHAHKYHFRMMANRQDGYAMVEASRGCPYQCTFCSQ
                     WKFWRDYRIRDPK"
BASE COUNT          523 a          471 c          470 g          424 t
ORIGIN      
        1 taacatcaac aaagctgact atctggatat tgccaagagg gtggatgcct ttattgatac
       61 ccagggaagg ttgcccaact acgcaagcag tatccttggt aaaataaggt cctaaccctg
      121 ctggacatgt tttctagggt actcagtttc caacaagaca accacccctg cccaactacg
      181 tatcagtaac accaggggca gcgtcaacca gcactgatat ggcagtgccg gattcattga
      241 aaagtaccgg gctccacggc aaattgtgag gtgactaata cccagatcca ggccctggca
      301 gcatccatag tcagtggaaa aacttccagc tacgctagag ccgaggccat cttcaactgg
      361 gtaagggata aggttggcta ctccttctac tacaacacca aaaatggtgc agtgaagacc
      421 ctcaacaccc tggccgggaa ctgtgtggac accagcaacc tcttggtagc cctgtgccgg
      481 gcagtgggaa ttccagcccg ataccagcat gggacctgta agttcaccag tgcaacctac
      541 ggccatgtgt gggtgcagat atggataaac ggggtatggt acaatgctga tgcatccagt
      601 tccaggaatt tcctgggagt ggttaagagc tggaccctgt ccaccttgaa gggcacctac
      661 acgtctttgc cattctagag gaaaaaaacg tgaggtgaga tgatgatcaa aaaaggaata
      721 attgccatgg tagccattgt agccgtggtg ggcctggtat acgcagccta cgatgatcca
      781 ggaaaggatg ccagcaacga ctctgagtac atgaagcacc cggaccatga taactccagt
      841 gacgatagca ccctaatctc agctagcctg gctaaaaagt tggcagaaag gtacattgaa
      901 gaaccaggag caacccctgg cacccctcaa ctattaacca gtggcaagaa aaggatctac
      961 ctggtaccag ttatctatca gggagaaaaa gtgggggaga ttgaaataga cgcagaaact
     1021 ggaaaaaacc tgggaggatc tggaggggct ccttgaagct ctttaactag gttccttaac
     1081 aatctattcc atcccccttt attttttttt aaaaaaaatg gctaaagtaa gaattttttt
     1141 gttttttcac aaattgtttt acagtagatc aacttaaaat atctacagaa aaccatgaag
     1201 atcctgttcg tggagccgcc caaggaattc tggtttgtga tgggcgagta tctgccacca
     1261 cctctaggta tactccagtt agccgcattt ctggaagaaa agggtgacga ctgggatctg
     1321 gaagtcctgg actgtcaagc cgaggaattg aaatggagtg gtctggaaag acgcctggaa
     1381 tctgccaacc cagacctgct cgtccccagt gccctggcca cctgtaacgc ctacaccatc
     1441 ctccgcaccg tttctctggc gaaaaagata gatccggaca ttaaaactgt ggtagggggt
     1501 cagcacttca cggccctggc ccaggagagc ctggaacagt acccggagat cgattttata
     1561 gtacgcggcg agggggagtt aaccctcctg gagttggtga gtagtttaga tcgggaaaag
     1621 tctcaactta acatcccggg actgtccttc cgccacaaga ataaaatcgt acacaatccg
     1681 ccccgtcctt tgatcaaaaa tctggatgat ttaccactgc ctggttatca tttcatcgag
     1741 gaccatgccc acaagtatca cttccggatg atggccaacc gccaggatgg ttacgctatg
     1801 gtggaggcct cccggggctg cccataccag tgcaccttct gctcccagtg gaagttctgg
     1861 cgggactacc ggatccgtga ccccaaga
//
LOCUS       DAOQVW010000322         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_79764_length_1884_cov_2.483324, whole genome shotgun sequence.
ACCESSION   DAOQVW010000322 DAOQVW010000000
VERSION     DAOQVW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79764_length_1884_cov_2.483324"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKK85_06955"
     CDS             complement(<1..328)
                     /locus_tag="PKK85_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide reductase"
                     /protein_id="HNS25790.1"
                     /translation="MAEEKKQETTEEPKIGVYTCHCGINIGGVVDIQEVVDYAASLPN
                     VIVSEEYKYFCSDPGQEMIQKDIKEKGVNRVVVAACSPRLHEPTFRRCIREAGLNPFL
                     FEFANIR"
     gene            complement(560..1495)
                     /gene="radA"
                     /locus_tag="PKK85_06960"
     CDS             complement(560..1495)
                     /gene="radA"
                     /locus_tag="PKK85_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012954923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HNS25791.1"
                     /translation="MVELEDLPNVGEKTAQKLRDAGFADMMRLATATAKELSVKVEIG
                     EGVAEKVIEAARKAEQIDFETALDVMERRKDVGRITTGSSGLDELIGGGIETQAITEV
                     FGEFGSGKSQISHEIAVSVQLPPEKGGLEGECVFLDTENTFRPERIRQIAEGFDMDVE
                     EVLQKIHIARAFNSSHQILMADKINELIQRGVNIRLVIVDSLTAHFRAEYVGRESLAT
                     RQQKLNQHLHTLQNIANTYNAAVFVTNQVQARPDAFFGSPTKAIGGHVLGHAATYRIW
                     LKKGLAGKRIARLVDSPHLPEGEAVFKIVTEGITD"
     gene            complement(1534..>1884)
                     /locus_tag="PKK85_06965"
     CDS             complement(1534..>1884)
                     /locus_tag="PKK85_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein A"
                     /protein_id="HNS25792.1"
                     /translation="CPSCNKRVIWEEDTYVCEVCGEKVETPEHLMIISIMIEDDTGSI
                     RTTFFRQAAEELLGMSTKDAEEIIDKTGDEGSLEEKVAELIGKQIKISVDANFDEYNE
                     EVRLNAKKVLDVKL"
BASE COUNT          467 a          442 c          361 g          614 t
ORIGIN      
        1 cccggatgtt agcgaattca aataagaatg ggttaagtcc agcttcccgg atacatcttc
       61 tgaaggtggg ttcgtgaagc cggggtgaac acgcggccac tactactctg ttaactcctt
      121 tttctttgat atctttctga atcatttctt gtccagggtc agaacagaag tacttgtact
      181 cctcggatac tatcacgttg ggcagggatg ctgcgtagtc caccacttcc tggatgtcta
      241 ccacacctcc aatgttgatc ccgcaatgac acgtgtagac tcctatttta ggttcttcgg
      301 tcgtttcttg ctttttttct tctgccaatt atatcaccta attccatgct tgtgaaggtt
      361 atatgttagg tagtgttgtg tctatcacat tgttaaataa agctttctat tgaaaataga
      421 gaacaaggtt tatatactag aaactggggg tgaggttaag atttaataaa aaactacaac
      481 agcactcttt agatagatga caatcaaaat tattgattat caagcatgtg acgtccatta
      541 aaaaaatagg tgtgggaatt taatccgtga ttccctcggt aacgatttta aaaacagctt
      601 ctccttctgg taagtgggga ctgtccacta atcgggctat tcttttacca gctaggcctt
      661 ttttaagcca gatccggtag gttgcagcgt gtcccaggac atggcccccg atggccttag
      721 ttggacttcc gaagaaggca tcaggccggg cttgcacctg gttagtcacg aatacagcag
      781 cattgtaggt gttagcgatg ttttgcaggg tgtgcagatg ttgattcaat ttttgctggc
      841 gggtggccag agattctcgt cccacatatt ccgcccggaa gtgggcggtg agtgaatcca
      901 ctattaccaa tcgtatgttc actccacgct gaatgagttc attaatcttg tcagccatca
      961 gtatctggtg gctggagtta aaggcccggg caatatgtat cttctgtaaa acctcttcca
     1021 catccatgtc aaatccctca gcgatctgcc gaattctttc gggacggaaa gtattttcag
     1081 tatctaagaa tacgcattcc ccttctaatc cccctttttc tgggggaagc tgaactgaaa
     1141 ccgctatttc atgggatatc tggctctttc ctgatccgaa ctctccaaaa acttcggtga
     1201 tggcttgagt ttcaatccct ccgccaataa gttcatccaa tccactactc ccagttgtta
     1261 tcctcccaac atcttttctt ctttccataa catccagggc agtttcaaaa tctatttgtt
     1321 cagctttacg ggcggcttct atcacttttt cggccacacc ctccccaatc tcaaccttaa
     1381 cactaagttc tttagcagtg gcggtggcca gcctcatcat gtctgcaaaa ccagcatccc
     1441 ttaatttttg agctgttttt tcaccgacat ttggtaaatc ttccagttca accatttaaa
     1501 tctccttcct gtttataaat tacatattca atttcatagt tttacatcga gaactttttt
     1561 ggcattcaga cgcacttctt cattgtattc atcgaaatta gcatctacac taattttaat
     1621 ttgttttcca attaattcag cgactttttc ttccagagag ccttcatcac cagttttatc
     1681 aataatttct tccgcatctt tggtgctcat gcccaacagt tcttcagctg cttggcggaa
     1741 gaacgtggtg cgtatggagc cagtatcatc ctctatcatg atagaaatta tcatcagatg
     1801 ttctggagtt tcaacttttt ctccacatac ttcacatacg tatgtatctt cttcccaaat
     1861 aactcgtttg ttacaagatg gaca
//
LOCUS       DAOQVW010000323         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_80097_length_1879_cov_2.839912, whole genome shotgun sequence.
ACCESSION   DAOQVW010000323 DAOQVW010000000
VERSION     DAOQVW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80097_length_1879_cov_2.839912"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..543)
                     /locus_tag="PKK85_06970"
     CDS             complement(112..543)
                     /locus_tag="PKK85_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS25793.1"
                     /translation="MKELISKPELCDECGKCERICPKNAIRVVSGVPLFCLHCAEDRA
                     PCLAVCPEGAIEDVDGAIIIHEDDCIGCGLCRDFCPVGAIQLNEYGIATKCDLCQGEE
                     VPLCVSICPTKALKVSSEDILAEKRDRVARELEKVKMIMKY"
     gene            complement(546..1049)
                     /locus_tag="PKK85_06975"
     CDS             complement(546..1049)
                     /locus_tag="PKK85_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS25794.1"
                     /translation="MEKIIIQPELCDGCLDCQDACAQIYGTSRILVREVEGSYYPIIC
                     QQCEDAPCELICPTEAISEGGIDEERCIGCGLCLMVCPFGAVTIHERKAHKCHQCPDR
                     DTPACIKACSQRAIAKVDTDQLQAQRQLEHIKKISSMKKKTRRGSELIHIVTANSRAK
                     KALDKEA"
     gene            complement(1061..>1879)
                     /gene="porB"
                     /locus_tag="PKK85_06980"
     CDS             complement(1061..>1879)
                     /gene="porB"
                     /locus_tag="PKK85_06980"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HNS25795.1"
                     /translation="CAGCGATIGVRLALKALGKNTVAVSATGCLEVITTPYPETAWEI
                     PWIHVAFENAAAVASGVERALKSQGKDAQVVAFAGDGGTADIGLQALSGAMERGHKII
                     YICYDNEAYMNTGVQRSGATPYGASTTTSPHGKESFGEDKPKKNIPMIMAAHGVPYVA
                     TASISYPEDFVKKVQKAKETEGPAYIHLHQPCTTGWGYDPSKTIELGRLAVETGSWIL
                     YEIVDGEFRVTYRPLQRKPVDEYLNAQKRFKHLSEQERLRIQNQVNALCSELKI"
BASE COUNT          407 a          505 c          450 g          517 t
ORIGIN      
        1 ctttttcaca tctgaaggga gataaataac tgctttttta aagaggcggc atacctcctc
       61 ttccactctc tcctggctta tcataaaaat caggtaaaat ctaggagatg atcagtattt
      121 catgatcatc ttaacttttt ccagttccct ggccacgcga tctcttttct cggcgaggat
      181 atcctcagag cttactttaa gggctttggt gggacatatt gacacgcaga gtggtacctc
      241 ctctccctgg cacaggtcac atttggtggc gattccatac tcattgagtt ggatagctcc
      301 cactggacag aaatcacgac acaaaccaca tcctatgcaa tcatcttcat ggatgatgat
      361 ggccccgtcc acgtcttcta tagccccttc cggacagact gccaggcaag gagcccggtc
      421 ctcggcacag tgcaagcaga ataagggaac tccactcacc actcttatgg catttttggg
      481 acatatgcgt tcacatttac cgcattcatc acatagctcc ggcttggaaa tcagttcctt
      541 catattcagg cctccttgtc cagggctttc ttggcccggc tgttggcagt gacaatatgg
      601 atcaactccg aacctcgccg ggtcttcttc ttcatactgg agatcttctt aatatgttcc
      661 aactggcgct gggcctgtaa ctggtccgtg tctaccttag caattgctcg ctgggaacag
      721 gccttgatac aggccggagt atctcgatcg ggacattgat ggcacttatg ggcttttcgc
      781 tcgtggatgg tcactgctcc gaagggacat accatcaggc agagaccgca acctatgcat
      841 cgttcttcat ctatccctcc ctcactaatg gcctcggtgg ggcagatgag ttcgcaggga
      901 gcatcttcac attgctggca gatgatggga tagtacgatc cttccacttc ccgaaccaga
      961 attctggagg tgccataaat ctgggcacag gcatcctgac agtccaggca gccatcacag
     1021 agttcaggtt ggatgattat tttttccaag gctgagcctc ctatattttt aattcactgc
     1081 acagggcatt aacctggttc tggatgcgca ggcgttcctg ttcactgagg tgtttgaacc
     1141 ttttctgggc gttgaggtac tcgtccacgg gtttccgctg caggggacgg taggtcaccc
     1201 tgaactcccc atccactatc tcataaagta tccaggaacc tgtttcaaca gctaatcggc
     1261 ctaattctat ggtttttgag ggatcataac cccaccccgt ggtgcaaggc tggtgaaggt
     1321 gtatgtaggc gggtccttcc gtttccttgg ctttctgcac ctttttcaca aaatcttccg
     1381 ggtatgatat ggaggcagtg gccacgtagg gcactccatg ggcggccatg atcatgggga
     1441 tgttcttttt tggtttgtct tcaccaaaac tctccttacc atgggggctg gtggtggtgg
     1501 aggccccata aggtgtggct ccactgcgct gcaccccggt gttcatgtag gcctcgttat
     1561 cgtaacagat gtagattatc ttgtgtcccc tctccatggc tcctgatagg gcctgcaacc
     1621 caatatctgc agttccacca tctccagcga atgcaactac ttgggcatct tttccctgtg
     1681 atttaagggc tctttcaaca ccagatgcaa ctgccgcagc gttttcaaag gccacatgta
     1741 tccagggaat ctcccaggca gtttctgggt aaggggtagt gataacctcc agacagccag
     1801 tggcagacac agccacggtg ttcttgccaa gtgctttaag ggccagtcta acacctatcg
     1861 tggctccgca gcctgcaca
//
LOCUS       DAOQVW010000324         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_80769_length_1869_cov_3.364939, whole genome shotgun sequence.
ACCESSION   DAOQVW010000324 DAOQVW010000000
VERSION     DAOQVW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80769_length_1869_cov_3.364939"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..578
                     /locus_tag="PKK85_06985"
     CDS             153..578
                     /locus_tag="PKK85_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNS25796.1"
                     /translation="MVDLPDEAVNMFNNEDCDKNRPLVWISTIGVKDNEEIPHLAPVC
                     FTKVLDRDKILVAINFATQTMYNIETYSRVALGIAVHYEGYLVKGTGAIIEKGSEFQQ
                     VQGMVKSRFGDKIKPRAALLINVEEVYSLKPGAGSKRVV"
     gene            638..982
                     /locus_tag="PKK85_06990"
     CDS             638..982
                     /locus_tag="PKK85_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HNS25797.1"
                     /translation="MDDGLKLLIYFLVGGSILTLVTYFGTRDRGILAAFIAMFPAVTT
                     MSIITIYSEAGGLLVLDYVKGLLLLSPVWMVFLLCVYFFLPKQGLLVALGAGIMVYIL
                     VAVFLFYIQPTI"
     gene            complement(983..1798)
                     /locus_tag="PKK85_06995"
     CDS             complement(983..1798)
                     /locus_tag="PKK85_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25798.1"
                     /translation="MKRDIIKIDQEKCTGCGDCIPGCPEGALQVIDGKAHLISDLFCD
                     GLGACIGTCPQGAIEVESREAEPYDEYRVMENVVKGGPNVIKAHLKHLDDHSEKELLS
                     QAIDFLKEKNMEIPEYQEEKTFACGCPGSMEVDLSTRPQSEDQAVVLSAELRNWPVQL
                     QLLNPQAPYLKNADLLIAADCAPFAYANFHQRFLKDKVLIILCPKLDQTLDQYVDKLA
                     EIFAYQDIKSISIVHMQVPCCSGIEVLVRRALEKAQKNIIVKDYTISINGEII"
BASE COUNT          474 a          415 c          445 g          535 t
ORIGIN      
        1 tgctccccta aaaatactac ttcaagattt ttacatggta taggtgcgat tgaaccattt
       61 aatatttctt atttggatct taaaccaagt tgaacaatca gatagatata ctgcggccaa
      121 taaaaatata tgcaaagtag ggggtgaaga gaatggttga tcttccggat gaagcagtga
      181 atatgtttaa caacgaagac tgtgataaaa ataggccttt agtatggatt tcgactattg
      241 gggttaagga caatgaggaa attccacatc tggccccggt atgctttacc aaggtgctgg
      301 atagggataa gattctggtg gccataaatt tcgctaccca gaccatgtac aacatcgaaa
      361 cctactccag ggtggccctg ggcatcgcag tgcactacga aggttacctg gttaagggaa
      421 ccggagcaat aatagaaaaa ggatctgagt ttcaacaagt ccagggaatg gtgaagtcac
      481 gttttgggga taaaataaag ccaagggcag cccttttaat caacgtggag gaggtttact
      541 ccctgaaacc aggcgctggt tctaagcgag tagtttagaa cagctacttg gtatacaatg
      601 cacagtattt cccgatccac ctactggaga tctattaatg gatgatggat tgaaattgtt
      661 aatctacttc ctggtggggg gctccatcct caccctggtc acctacttcg gtactaggga
      721 cagggggatc ctggctgcct tcatagccat gttcccggcg gtgaccacca tgagcatcat
      781 caccatctac agtgaagccg ggggcctgct ggttttagac tatgtgaagg gactcctgct
      841 gttaagtccg gtgtggatgg tgttcctatt atgtgtttac ttctttctcc ccaaacaagg
      901 gttgctggtg gcattggggg caggaattat ggtatatatt ctggttgctg ttttcttatt
      961 ttatatacaa ccaaccatct aactagatta tctctccatt tatagaaatg gtgtagtcct
     1021 tgacgattat gttcttctgg gccttttcca gggcccggcg taccaggact tcaataccag
     1081 agcagcatgg aacctgcatg tgcacgatgc tgatggactt tatgtcctgg taggcgaaga
     1141 tctcggctaa cttgtccaca tactggtcca gggtctgatc cagcttggga caaaggatga
     1201 tgagaacctt atcttttaag aagcgctggt ggaagttggc gtaggcaaag ggggcgcagt
     1261 ctgctgcgat gagtaaatct gcattcttca gatagggcgc ctggggattt aacagttgca
     1321 actgcactgg ccagttacga agctcggcac tgaggaccac ggcttggtct tcactctggg
     1381 gacgggtgct tagatccacc tccatggagc cgggacaccc gcaggcaaag gtcttctctt
     1441 cctggtactc tggtatctcc atattttttt ccttcagaaa gtctatggcc tggctgagga
     1501 gttctttctc actgtgatca tccaagtgtt tcagatgggc ttttataacg ttaggtccac
     1561 cttttacaac gttttccata accctgtatt catcataggg ctcagcttcc ctggattcta
     1621 cttcaatggc gccctggggg caggttccga tacaggcacc cagaccatca cagaaaaggt
     1681 cactaataag gtgggccttc ccgtcaatta cctgcagggc accctcagga catcctggaa
     1741 tacagtcccc acagccggta cacttttcct gatcaatttt gatgatatct cgtttcataa
     1801 tcttcatctc cgcatcttaa ttcttttttt tctaattttc tttaaaaaac attataaaat
     1861 ctagttcat
//
LOCUS       DAOQVW010000325         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_80799_length_1869_cov_2.546307, whole genome shotgun sequence.
ACCESSION   DAOQVW010000325 DAOQVW010000000
VERSION     DAOQVW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80799_length_1869_cov_2.546307"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PKK85_07000"
     CDS             <1..1059
                     /locus_tag="PKK85_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNS25799.1"
                     /translation="ALNSRNYKGQDPNRIANKKATPGYNIIQFARNNGVQYLLDVHSG
                     SQVGSKGYIFVSNPSTITVKEKNWSNHIKSKTGCYISTNGADDAGMVRNCAKKYSINT
                     ITLEVERDSIPTMTSANVEYKMIQAAVNYLGFPADSYGPKITSTNPAKDALGVPLTSA
                     VTITFNKNIKSSANFSGIYIKNLTTGKKVSLASKTISGNTLTLKMASSRIKNNVYQVY
                     IPAGAVKDGSGDNLVLTYSYSFSTTPDTVSPKVSSTKPANKTTGIPLTSAVTITFNKN
                     IKSSANFSGIYIKNLTTGKKVTLASKTISGKTLTVKMTYSRLRNNVYQVYIPASAVKD
                     ATGNNLKASYSFQFQTVK"
     gene            1225..1725
                     /locus_tag="PKK85_07005"
     CDS             1225..1725
                     /locus_tag="PKK85_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25800.1"
                     /translation="MGLLKNEDNEQLRQLVKACLLEISKLKIELKKCQKESSKVGDDR
                     ALLELKEDVESKLLEKEKEINNLQELLEKKDQKLEELEIIRYHFNALTAKPKKDLTSF
                     QSQVYQLLPAEENTLENLYTHITEIGFSELSSENFEHVIRNLERKGYFESYQKDDEIY
                     WQKIDK"
BASE COUNT          669 a          390 c          335 g          475 t
ORIGIN      
        1 gctttaaatt cacgtaacta caaaggacag gatcccaacc gaattgcaaa taaaaaggca
       61 actccgggct ataacataat ccagttcgcc agaaataacg gggttcaata tctgctggat
      121 gtgcattctg gtagtcaagt aggttctaaa gggtatatat ttgtaagtaa tccatccacc
      181 ataacggtca aggaaaaaaa ctggagtaac catatcaaat ctaaaaccgg ctgttatatt
      241 tccaccaatg gtgcagatga tgcgggtatg gtacgtaatt gtgcaaagaa gtatagtatc
      301 aacaccataa cattggaagt agaaagagat tccatcccca ccatgacctc agctaacgtg
      361 gaatataaga tgattcaggc tgctgttaac tatctgggtt tcccagccga ctcttacgga
      421 cccaaaataa cctccacaaa tccggcaaaa gatgccttag gggtccctct tacctctgca
      481 gtcaccatca ccttcaacaa gaacataaag tccagtgcca atttctccgg aatatacatc
      541 aaaaacttaa ccaccggtaa aaaggtatcc ctagcatcca agaccatcag tggaaatact
      601 ttaaccttga agatggcatc cagccgcata aaaaacaacg tttatcaagt atatatacca
      661 gccggtgcag ttaaagatgg atcgggagac aatttagttt taacttactc ttactcattt
      721 agcaccacac cagatacagt ttcccctaaa gtttccagca ccaaaccagc caataaaacg
      781 acgggcattc cacttacctc tgcagtcacc atcaccttca acaagaacat aaagtccagt
      841 gccaatttct ccggaatata catcaaaaac ttaaccaccg gtaaaaaggt gaccctggca
      901 tccaagacca tcagcgggaa aacgttaacg gtgaagatga cctacagccg tttgaggaat
      961 aacgtttacc aagtttatat accggccagt gcagtgaagg atgctacagg aaacaatctt
     1021 aaagccagtt acagtttcca gttccagaca gttaaataaa tttacataaa catctatttt
     1081 caaatttaaa taaaataaca atctagatat ttattgaatt taactatttt accatcattt
     1141 ctatttaaaa tcaaaataat gatttaaagg acctaatact aaactttaaa aggcaattac
     1201 tatataattt aatacagtgg tacgatggga ttgttaaaga acgaggacaa cgaacagttg
     1261 aggcagctgg tgaaggcctg tcttctggag ataagtaaac tcaagattga gcttaagaag
     1321 tgccagaagg aatcatccaa agttggtgat gatagagctc ttttagaact taaagaagac
     1381 gtggaaagta aattactgga gaaagaaaag gagatcaaca atctacagga actactagag
     1441 aagaaggatc agaagctgga ggaactggag ataattcggt accacttcaa cgcccttact
     1501 gccaaaccca aaaaagacct gacctccttc cagtcccagg tgtatcagct gttacccgct
     1561 gaagagaaca ccctggaaaa cctgtacaca catataactg aaatcggatt cagtgaactt
     1621 tccagtgaaa atttcgaaca tgtaatccgt aacctggaga gaaaaggtta ctttgagtct
     1681 taccagaagg atgatgaaat atactggcag aagatagaca aataagtaaa tttaccactt
     1741 atttttgaat aaatatctgg ttttgaattc aatatcctgt cccgggacga tttatcagtt
     1801 ccccatactt tagaagttat taattattgt taatataatc cctatacaca aactatttat
     1861 ataacctta
//
LOCUS       DAOQVW010000326         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_80851_length_1868_cov_2.938776, whole genome shotgun sequence.
ACCESSION   DAOQVW010000326 DAOQVW010000000
VERSION     DAOQVW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80851_length_1868_cov_2.938776"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKK85_07010"
     CDS             <1..690
                     /locus_tag="PKK85_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 2 glycosyl transferase"
                     /protein_id="HNS25801.1"
                     /translation="DWLRTLYQAVESSGGDVAGAGGPRLIAPTPSKKELVINAFITSL
                     VASGGNPAFSQRKVAYLESIPNYNAIYKKEIISKFRYDESLVISDDHELNYRLHQAGY
                     LFKYLPEARVYHRETDSVVQFTRNMFRYGFNMASLLRKHLHFFKVKVILTLTMLAYLI
                     LLVPLYLLLGSLALVPPLLYLVYAVLAWVEVLLRTRTLWSLMVFLLMPLQHLAYACGV
                     IYNFLTPHRRV"
     gene            709..1440
                     /locus_tag="PKK85_07015"
     CDS             709..1440
                     /locus_tag="PKK85_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS25802.1"
                     /translation="MNNDNAPETAEAFFQRYGRDIVVVLPAYNEDQTIARVMDDLLGW
                     GVQLVVVDDGSQDETYRIVQDYLKRYPGRVHLYRHLLNRGVGAALQTGLEAALKRRPR
                     VIVTFDSDGQHHAQDLYGVVRPVVMGQADAVIGTRDFQEMPLHKRFGNTVMNLITLLF
                     YGHQVTDSQSGFRAFTPQAARLMELHSSGYGICSEMVGEVFRHDLHLVEVPISTIFTD
                     YSLSKGTDTRIGLKILIKLIRDVFK"
     gene            1464..1823
                     /locus_tag="PKK85_07020"
     CDS             1464..1823
                     /locus_tag="PKK85_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HNS25803.1"
                     /translation="MEFHLYQYLGIIFGVVAFLFTLSRFRKGKLSLGMLALWSGLWII
                     VILVSIFPNATSQLALASGIGRGLDVVLILGLIGCYYLLFRMFNMMENLKEEMNYLIR
                     ELALQRGESGEKEEDEG"
BASE COUNT          409 a          538 c          517 g          404 t
ORIGIN      
        1 gactggctcc gcaccctgta ccaggccgtg gagagtagtg gaggggatgt ggctggtgcc
       61 ggcggccccc gactcatcgc ccccaccccc agtaagaagg aactggttat caacgccttc
      121 atcacctccc tggtggcctc gggtggtaac cctgccttca gccagcggaa agtagcctac
      181 ctggagagca tacccaacta caacgccatc tacaagaagg aaatcatttc taagttccgc
      241 tacgatgagt cactggtcat cagcgacgac cacgagttga actatcgcct gcaccaggcc
      301 gggtacctct tcaagtacct cccagaagcc cgtgtatacc accgggagac tgactctgtg
      361 gtgcagttca ccaggaacat gttccgctac ggcttcaaca tggccagcct cctgcgcaag
      421 caccttcact tcttcaaggt caaggtgatc ctgaccttga ccatgctggc ctacctgata
      481 ctcctagtgc cactgtacct cttactgggc agcctggctc tagtaccacc cctcctatac
      541 ctggtgtacg ctgtcctggc ctgggttgaa gttttactcc gcacccggac cctgtggtcc
      601 cttatggtat tcctgctcat gcccctgcag cacttggcct atgcctgtgg ggtgatctac
      661 aacttcctca ccccccaccg gagggtgtag aaataaggag aatatatatt gaataacgac
      721 aacgccccgg aaacagccga agccttcttc cagagatatg ggagggacat cgtggtggtc
      781 ctgccggcct acaacgaaga ccagaccata gctagggtta tggatgatct gctaggttgg
      841 ggggtgcaac tggtggtggt ggatgatggt tcacaggatg aaacctaccg catcgtccag
      901 gactacctga aacggtaccc tggacgggtg cacctctacc ggcaccttct aaaccggggg
      961 gtgggggctg ccctgcagac cggactggaa gccgccctta aaagaaggcc ccgggtcata
     1021 gtgaccttcg actccgatgg ccagcaccat gcccaggacc tgtacggggt ggtgcggccg
     1081 gtggtgatgg gccaggccga cgccgttatt ggcacccggg acttccagga gatgcccctg
     1141 cataagaggt tcgggaacac ggtgatgaac ctcatcaccc tcctcttcta cggccaccag
     1201 gtgactgatt cacaatccgg cttccgggcc ttcacccccc aggcagcccg gctgatggag
     1261 ctgcactcca gtggctacgg catctgctcg gagatggtgg gggaggtgtt ccggcatgac
     1321 ctccacctgg tggaggtgcc catcagcacc atcttcaccg actactcact ctccaagggg
     1381 acggacaccc ggattggcct gaaaatactc attaaactaa taagagatgt gttcaaatag
     1441 aatagtaaaa aggtggtatg tctatggagt ttcacttgta ccagtatctg gggatcatat
     1501 tcggggtggt ggccttcctc ttcaccctat cccggttccg gaagggtaaa ctgtccctgg
     1561 ggatgctggc cctgtggagt ggattgtgga tcatcgtcat cctggtgtcc atcttcccca
     1621 acgccacctc ccaactggcc ctggccagtg gcatcggccg ggggctggac gtcgtgctca
     1681 tactgggcct catcggctgc tactacctcc tgtttaggat gttcaacatg atggagaacc
     1741 tgaaggaaga gatgaactac ctcatccggg agctggccct ccagcgtggg gagagtggtg
     1801 aaaaagagga agatgagggc taaagaggaa aaatcgaaaa gttgaggatc tataattaat
     1861 tttttacc
//
LOCUS       DAOQVW010000327         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_81157_length_1864_cov_2.363184, whole genome shotgun sequence.
ACCESSION   DAOQVW010000327 DAOQVW010000000
VERSION     DAOQVW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81157_length_1864_cov_2.363184"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..266)
                     /gene="porD"
                     /locus_tag="PKK85_07025"
     CDS             complement(24..266)
                     /gene="porD"
                     /locus_tag="PKK85_07025"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorD"
                     /protein_id="HNS25804.1"
                     /translation="MVNTGAAVKEPGSTRQNKTGSWRTFKPVLDKDKCIDCDNCILFC
                     PEGCISQDHDIDYDYCKGCGICAEECPVEAIRMERG"
     gene            complement(285..809)
                     /gene="porC"
                     /locus_tag="PKK85_07030"
     CDS             complement(285..809)
                     /gene="porC"
                     /locus_tag="PKK85_07030"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorC"
                     /protein_id="HNS25805.1"
                     /translation="MIEIRFHGRGGQGAVTAAEILAKAAFEDGKYCQAFPFFGAERKG
                     APVMAFSRIHDKPIRRRYQVYNPDYVVVLDETLQEAVDIISGLKEGGKVIVNSEEDLK
                     LDGVEVHTIDATGIALETLGVPIVNTVMLGAFAKVTEEVSLDSIIKITKETFPGKIGE
                     KNAIAAEIAYEKVK"
     gene            987..1313
                     /locus_tag="PKK85_07035"
     CDS             987..1313
                     /locus_tag="PKK85_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25806.1"
                     /translation="MSEWIDVERETENKEKDNIWEPETVGESIQGIYIEKEENVGQFK
                     SKMYFLKTEEGEKKIWGSTVLDDLMGKVPLGSEVRIIYQGKQPSKSGRQPWKDYKVQY
                     RSVKKS"
     gene            1394..>1864
                     /locus_tag="PKK85_07040"
     CDS             1394..>1864
                     /locus_tag="PKK85_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HNS25807.1"
                     /translation="MKVLIITAQLASPLVKKVTAQSEHEIHILTVETPIAAFLTPQRI
                     VAELKKVSGIELESLDMIITPGLLRKDVSPVEEAMAIPTYKGSTDAADLGVVLEMLDK
                     IDLSPKKPADKLIEDELRRRALKYIHDFEKDSENIKKLLKKPENIMVGSLPVGED"
BASE COUNT          568 a          376 c          386 g          534 t
ORIGIN      
        1 agtcataact ttcagtacca tctctatccc ctctccattc taatagcttc aactggacac
       61 tcttcggcgc atattccgca tcctttacag taatcgtaat cgatgtcgtg gtcctggctt
      121 atgcatcctt ctggacagaa aagaatgcag ttatcacagt ctatgcattt atccttatcc
      181 aggactggtt tgaatgttct ccagctcccg gttttgttct gccgggtgct gcctggttct
      241 ttaactgctg ctcctgtgtt taccattaga tcaccttaat agtcttattt caccttttca
      301 taggcaattt cagctgctat ggcgtttttc tcgccaattt tgcctgggaa tgtttcttta
      361 gttattttta tgattgaatc cagggaaact tcttctgtta ctttggcaaa cgctcctaac
      421 attactgtgt tcacaatggg gacgcccagt gtttccaggg caattccggt agcgtcgatg
      481 gtgtgaactt ccacaccatc caacttcaaa tcctcttcgc tattgactat tactttgccc
      541 ccttctttaa gcccagaaat tatgtccacg gcttcttgta atgtttcatc cagtactact
      601 acgtaatcag gattataaac ttgatatctc cttctgatag gcttatcatg tattcttgaa
      661 aaagccatta ctggtgcgcc ttttctttca gcaccgaaaa atgggaatgc ttgacagtat
      721 tttccatctt caaaggctgc ttttgctagt atctctgcgg cggtcactgc tccttgcccg
      781 ccacgtccgt gaaagcgaat ttcaatcatc tatttacctc cattcctcat agttaatcat
      841 tataaatttt taatatatat aagtgttgga tgaagtttga tatattaaag ggacttattt
      901 catgaaaaaa aatagtagca atgaataaaa aaaatggaaa ctaaattaac gtctttgatt
      961 ataccaatac tactaggggg atattcatga gtgaatggat agatgttgaa agagaaactg
     1021 agaacaagga aaaagataat atttgggaac cagaaactgt tggagaatct attcagggta
     1081 tttacataga aaaagaagag aatgtggggc agttcaagag taagatgtac ttccttaaaa
     1141 ccgaggaggg agagaagaaa atctgggggt ccactgttct ggatgatctg atggggaagg
     1201 ttcctctggg cagtgaggtg cgcatcatat atcagggcaa acaaccctca aaatcaggca
     1261 gacaaccctg gaaggattac aaagtacagt accgatcagt gaaaaaaagt tagaaccaga
     1321 ttaaaaaaga gaaactggct tagtatctag gataagataa tggtaaaaac tggagtcttc
     1381 aaggaataag cttatgaaag tactcatcat cacggcccaa ctggccagtc ccctggtaaa
     1441 aaaggtcaca gcacaatcag aacatgaaat ccatatttta actgttgaaa cgcctatagc
     1501 tgcattttta acacctcagc ggatagtggc tgagttgaag aaagtttcag ggatagaact
     1561 tgagtcactg gatatgataa tcacccctgg gcttttaaga aaggatgtca gtccagtgga
     1621 agaggccatg gccataccta cctacaaggg atccacagat gcagctgatc tgggagtggt
     1681 cctggagatg ctggacaaaa ttgatctgtc accaaaaaaa ccggcagata agcttataga
     1741 agatgagcta cggcgaagag cacttaaata cattcatgac tttgaaaaag actctgaaaa
     1801 tattaaaaaa ctactaaaaa aacctgaaaa catcatggtg ggaagcctcc ctgtggggga
     1861 ggac
//
LOCUS       DAOQVW010000328         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_81187_length_1863_cov_3.563053, whole genome shotgun sequence.
ACCESSION   DAOQVW010000328 DAOQVW010000000
VERSION     DAOQVW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81187_length_1863_cov_3.563053"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PKK85_07045"
     CDS             <1..1063
                     /locus_tag="PKK85_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNS25808.1"
                     /translation="DHNVPANNAGSAEFQRITREFAREQGIKNIYTHGEGICHQILPE
                     KGYIKPGTVVVGADSHTCTYGAFGTFATGMGATDMAAVFANGKTWFMVPSAYKIIVNG
                     MLSAYVTAKDVILYIIGEIGSDGATYKALEFGGDTIDFMDVSGRMTIANMVIECGAKN
                     GIMEPNRATLDYLKKRGVAGFDVIKSDTDSHYEREYHFQVHDLEPQIACPHNVDNVRP
                     VSKVSGTHIDQAVIGSCTNGRLEDLHIAADVLKNKTVHPDVRLIVVPASRQIYLDAME
                     EGIIKTFLNAGALVCNPGCGPCLGAHMGVLTEGEVCVATTNRNFVGRMGDPNSEIYLA
                     NPAVVACSAIYGEIKDPRE"
     gene            1065..1598
                     /locus_tag="PKK85_07050"
     CDS             1065..1598
                     /locus_tag="PKK85_07050"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNS25809.1"
                     /translation="MDKTILNGIKEIEKSLGNLSSAQKILLTTDGSVTTILDVLKGHV
                     KIKTLIQEFRDADEEMAHLLQIKVGDPVNYRVVVIQRDEPLIHAVSLIPVDRLDNDFR
                     EDLIRADIPIGRILKKHQIESRREIKSVTVEESSPEMVEIFNDAAPVLSRTYNIIHND
                     QVLIWIKETFPSSLFVE"
     gene            1648..>1863
                     /locus_tag="PKK85_07055"
     CDS             1648..>1863
                     /locus_tag="PKK85_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515626.1"
                     /note="FEN-1; Rad2; similar to eukaryotic enzymes;
                     endonuclease that cleave the 5'overhanging flap structure
                     that is generated by displacement synthesis when DNA
                     polymerase encounters the 5'end of a downstream Okazaki
                     fragment; as 5'endo-/exonuclease and
                     5'pseudo-Y-endonuclease activities; cleaves the junction
                     between single and double-stranded regions of flap DNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap structure-specific endonuclease"
                     /protein_id="HNS25810.1"
                     /translation="MGVKFRDIVSTEHIKFEDLDGKTVALDAANIIYQFLSSIRQRDG
                     TPLMDHNGRVTSHFSGILYRTTSLIEKG"
BASE COUNT          562 a          364 c          442 g          495 t
ORIGIN      
        1 cgatcacaac gtacctgcta acaacgctgg atctgcagaa tttcagagaa ttaccaggga
       61 atttgcccga gaacagggta ttaagaacat ttatacccat ggcgagggta tatgtcatca
      121 aattctgcct gaaaagggtt atattaaacc tggaacagtt gtagttggtg ctgactccca
      181 tacttgtact tatggggctt tcggtacctt tgctacaggt atgggggcca ctgacatggc
      241 tgcagtgttt gccaatggaa aaacttggtt catggtccca tctgcttata aaatcatagt
      301 aaatggaatg ttatcggcct atgttactgc taaggatgtt attttatata ttatcggcga
      361 aattggatca gacggtgcca cctacaaggc actggagttt ggtggagata ccatagactt
      421 tatggacgta tcagggcgga tgaccatagc caacatggta attgaatgtg gggccaaaaa
      481 cggcataatg gaacctaacc gggctacatt agattatttg aagaaaagag gcgtggcggg
      541 ttttgatgta attaaatcag atacagactc acactatgaa cgtgaatacc atttccaggt
      601 tcatgatctg gaacctcaaa tagcctgccc ccataatgta gataatgtgc ggccggtttc
      661 caaggtcagc ggaacccaca tagaccaggc agttatcggt tcatgtacca atggccgttt
      721 agaagatctg catattgcag ctgatgtttt aaagaacaaa acagtacacc cagatgttcg
      781 tctgattgta gtgcctgctt cccgccagat atatctggat gcaatggaag aggggatcat
      841 aaagactttt ctcaatgcag gtgccctggt atgtaatcct ggatgtggac cctgccttgg
      901 tgcacatatg ggggtgctca ctgaaggcga ggtctgtgta gccaccacca accgaaattt
      961 cgttggacgt atgggtgatc ctaattcaga aatttaccta gccaatcccg cggtagtggc
     1021 ttgttcggct atttatggtg aaataaaaga tccaagggaa taaaatggat aaaacaatct
     1081 taaatgggat aaaggagatt gagaagagtt taggaaacct ttcaagtgct caaaaaattc
     1141 ttctcactac agatggatct gtaacaacca tcctggatgt tttaaaggga catgtgaaga
     1201 taaaaacctt aattcaggaa tttcgtgatg cggatgaaga aatggctcat ttactgcaga
     1261 ttaaagtggg tgatccagtc aactatcggg ttgtggtcat ccaacgggat gagcccctga
     1321 tacatgccgt ttccctaatc ccggtggatc gtctagacaa tgattttagg gaagatctca
     1381 ttagggctga cattcccatt ggacgtatac tgaaaaaaca ccagatcgag tcccgccggg
     1441 agataaagtc agtgacggtg gaagaatcaa gtccagaaat ggttgaaata ttcaatgatg
     1501 cggctccagt tctaagccga acctacaaca taatacataa cgaccaagta ctgatttgga
     1561 taaaggaaac ctttccctca tccctatttg tagaatgaac ttgtagaatt aaggccggaa
     1621 tttttaattt aaatggtggt acaagctatg ggtgttaagt ttcgggacat agtatctacc
     1681 gagcacataa aattcgaaga tttagatgga aaaacagtgg cactggatgc agctaacatc
     1741 atataccagt tcttatccag catacgacag agagatggca caccccttat ggatcataat
     1801 ggaagggtaa cttctcactt tagtggtatc ctgtatcgca ccacatccct gattgaaaag
     1861 ggg
//
LOCUS       DAOQVW010000329         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_81402_length_1860_cov_3.346260, whole genome shotgun sequence.
ACCESSION   DAOQVW010000329 DAOQVW010000000
VERSION     DAOQVW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81402_length_1860_cov_3.346260"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKK85_07060"
     CDS             complement(<1..296)
                     /locus_tag="PKK85_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase subunit beta"
                     /protein_id="HNS25811.1"
                     /translation="MRARPRDFIYTQDDLFLATTNYLHPDDRILAFLRYIPKEDGERS
                     RNNARYTKVDSNQAYEYLKDFFPNYIFKCDLSQVEMMGVPNNRVKDILKPEKRL"
     gene            392..964
                     /locus_tag="PKK85_07065"
     CDS             392..964
                     /locus_tag="PKK85_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HNS25812.1"
                     /translation="MKYKGLKDSILYDGSQIEPMWALKNFNIKGSSIISWIGPMKLEN
                     NEILDYEDVGLEIRSDKMLHLIVEHFDIQPADLRLCYHRQRLLVTIVKDVLEQMGLKI
                     QRKGDDIFIGPGKLSVSIATCSASSMKIHFGVNLTTEGTPEDVTTAGLKDWIEDLDNE
                     KTQNIAEKIYKAYKMELYTIEEDISKTRVF"
     gene            976..1455
                     /locus_tag="PKK85_07070"
     CDS             976..1455
                     /locus_tag="PKK85_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNS25813.1"
                     /translation="MKIVINGIHANLRFASAHMIPGHEFCGGIHGHSYHVDVVVEGER
                     SGQFGFVVDFKAVKAIVRKLCKKLDHKVLLPLESDQITFTNMDESLEFSIGSKNYVLP
                     NEDCCLLPITSTSAEELSRFFAHYVHQALEDLGVNIEGVEICVNEGIGQGAHYTFRT"
     gene            1487..>1860
                     /locus_tag="PKK85_07075"
     CDS             1487..>1860
                     /locus_tag="PKK85_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNS25814.1"
                     /translation="MCEVFSSIQGEGTLLGRRQIFVRFAGCNLHCNYCDTPQSRDVKA
                     GYDISSALLYQKIEDLKTPDLHSISLTGGEPLLNVDFIKKFLKKNRLTCLLETNGSLP
                     GELKKIVDYLSYASVDIKLPGH"
BASE COUNT          591 a          321 c          407 g          541 t
ORIGIN      
        1 ttaagtcgtt tttcgggttt taatatatct ttaacccgat tatttgggac tcccatcatt
       61 tctacttggc ttaaatcgca tttgaaaatg tagttaggga aaaaatcttt tagatattca
      121 taagcttgat tggagtctac ttttgtatat cgagcgttat tacgcgacct ttcaccatct
      181 tctttaggaa tgtaccttaa aaaggcaagt atcctgtcat caggatgtag ataattggtg
      241 gtggccaaga agaggtcatc ctgggtgtag ataaagtctc ggggtctggc tctcatgaat
      301 agatttagtg atttgcaaca ttattaaata tggtagtcaa tatgatagtc atgagaagga
      361 ctattaagaa gccatgcata agtgtgaatt tatgaaatac aagggattaa aagactccat
      421 tctttatgat gggagtcaaa tagagcctat gtgggcatta aaaaatttca atatcaaagg
      481 ttccagcata atatcatgga taggccccat gaaactggaa aacaacgaaa tcttagatta
      541 tgaagatgtg gggttggaga taagatctga caagatgtta cacttgatag tggaacattt
      601 cgatattcaa cctgcagatc tgcgtctatg ttatcatcgt cagagactcc tggtcaccat
      661 tgtcaaagat gtcctggaac agatgggtct taagattcaa agaaagggtg atgatatttt
      721 cataggtccg ggaaagcttt cagtatccat agccacttgc tctgccagca gtatgaagat
      781 ccactttgga gtaaacctaa ccacagaagg aaccccggag gatgttacca ccgcggggct
      841 caaagactgg attgaggatt tggacaatga aaaaacacaa aacattgcag aaaaaattta
      901 taaagcttat aaaatggaat tatatacaat agaagaggat atatctaaaa caagagtgtt
      961 ttaggggtga tatgtatgaa aatagttatt aatggtattc acgccaattt acgcttcgca
     1021 tcagcccaca tgatcccggg ccacgaattc tgtggaggta tacacggcca ctcttaccac
     1081 gtggacgtgg tagtggaggg agagcgcagt ggacaatttg gatttgtggt ggatttcaaa
     1141 gcggtgaagg ccattgtgag aaaattatgt aagaaactgg accacaaagt tcttttaccc
     1201 cttgaaagtg accagatcac atttacgaat atggatgaat ccctggaatt ttccattggt
     1261 tccaaaaatt atgtgcttcc caatgaagac tgctgtttac tccccatcac ctctacctca
     1321 gcagaggaat tatctcgctt ctttgcccat tatgtccacc aggccctgga agatttaggt
     1381 gttaatatcg agggtgtgga gatatgtgtt aacgagggaa ttggacaagg agcccactat
     1441 acctttagaa cttaatgaat ctggagggat ttaatttaat actcgtatat gtgaagtttt
     1501 ctccagcata cagggtgaag gaactctttt aggccgacga cagatattcg taagatttgc
     1561 tggctgcaat cttcactgta attactgtga cacaccacaa agcagggatg taaaggcagg
     1621 gtatgatata tcctctgctc ttctatacca gaaaattgag gatctaaaaa cacctgatct
     1681 gcattcaatt tccttaactg ggggagaacc actactaaac gttgacttta taaaaaagtt
     1741 tttaaaaaaa aatagattaa cctgtttatt ggaaaccaat gggtcattac cgggtgaact
     1801 taaaaaaata gtggattatt tgagctatgc atcggtggat attaaattac ctggacatga
//
LOCUS       DAOQVW010000330         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_81605_length_1857_cov_3.679245, whole genome shotgun sequence.
ACCESSION   DAOQVW010000330 DAOQVW010000000
VERSION     DAOQVW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81605_length_1857_cov_3.679245"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1800
                     /locus_tag="PKK85_07080"
     CDS             274..1800
                     /locus_tag="PKK85_07080"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNS25815.1"
                     /translation="MEVEFLKDKEIIVASNRGPVLFKRDDRGKIELIRGAGGIVGSMI
                     PFMQKTRGTWVSSAIGECDHYMDHEYQGKVPIPLEDPEYYVQFIKTSEETYNGFNGKF
                     ANPLLWFIHHSMWNPPYSPCADDELHQAWDSYQYVNSRFAAAIGEDVRNSKKTPIVML
                     QDYHIYLTPRLIREEHPDVLMSQFVHIPFPPPEIFQQLPNHMQIEILESILTNDVLGF
                     HIPRYKNNFLRTVKQILSQALVDDMVGDILYNGHVCHVRTYPISIDLETIQKHAQNPM
                     VILKEKEVDELVGNCKLIYRTDRADLSKNIIRGFQAYDMFLEKYPEWKENVKFVATLM
                     SSRQDIKIYREYTDNIRDIVKKINEKHATPDWEPIKYICRGDYDLVVALLKRYDVLMV
                     NPILDGMNIVAKEGSAVNENNGALILSTGAGCYEELKDGALCINPYDIRQTAESINTA
                     LLMDEKTKKQLIKNARAVLTRNNLNKWVSDQLKDIEMVMYERQELEKSENHEEARLMN
                     "
BASE COUNT          631 a          352 c          401 g          473 t
ORIGIN      
        1 ttttgttggc cgctttgcta gagggaagaa aaataaaaaa gaggtttatt tttaaatccc
       61 agatgttttt cctaattatc attaaaacca gatagtatac taaaagttta aatagaggat
      121 aattaataat atcactcaaa aaaaggtagt aggatggaga tagcgccagt tggagggata
      181 agatttactg tttaggtagt cttactttca attggcttct agataatgca cactgaaatt
      241 tccactaaaa tcaatgtaat cggaggcacc tgaatggaag ttgaattttt gaaagacaaa
      301 gaaataatag tagcctcgaa tcgaggtcct gtattattta aaagagacga cagagggaaa
      361 atagaattaa tcaggggggc ggggggaatt gtgggatcca tgattccctt catgcaaaaa
      421 actcgtggta catgggtttc ttctgcaatt ggagaatgtg accattatat ggaccatgaa
      481 tatcagggta aagtacccat accattagaa gaccctgaat attatgtcca attcatcaaa
      541 acctcagagg aaacatacaa tggctttaat ggtaaatttg ccaacccatt gttgtggttt
      601 attcatcatt ccatgtggaa cccgccctac tcaccctgtg ccgatgatga actgcaccag
      661 gcctgggatt cctaccagta cgtgaactcc agattcgcag ctgccatagg ggaagatgtg
      721 cgaaactcaa aaaagacccc catagtaatg ttacaggatt atcatattta tttaaccccc
      781 agattgataa gagaagagca tccggatgtt cttatgagtc aattcgtcca cattcctttc
      841 ccgccaccag aaatattcca gcaactgccc aaccacatgc aaatagaaat tttagagagt
      901 atactgacca atgacgttct ggggttccat attccacgtt ataagaacaa ttttctccgg
      961 actgtaaaac aaatcctgtc ccaagccctg gtggatgaca tggtgggcga cattctgtac
     1021 aacggacacg tctgccatgt gcgaacctat cccatcagta tcgacttgga aacaattcag
     1081 aaacatgccc agaaccctat ggtgatttta aaggaaaaag aggtggatga actggttgga
     1141 aactgcaagt taatctacag gactgaccgg gcagaccttt ctaagaatat tatcagggga
     1201 ttccaggcct atgacatgtt cctggagaag tacccagaat ggaaagaaaa tgttaaattt
     1261 gtggccactt taatgtcatc ccggcaggac atcaagatct acagggaata caccgataac
     1321 ataagggata tagttaaaaa gatcaacgaa aaacatgcta ctccggattg ggagccaatt
     1381 aaatacatct gccggggaga ctatgatttg gttgttgccc ttcttaaaag atatgatgtt
     1441 ttaatggtca accccatcct ggatgggatg aacatcgtag ccaaagaagg gagtgcagta
     1501 aacgaaaaca acggggcttt aatcctatct accggggctg gatgttatga agagttgaaa
     1561 gatggagccc tctgcataaa tccctatgat atacggcaaa ctgcagaatc cataaacacc
     1621 gccctgttaa tggatgagaa gaccaaaaag cagttgatca aaaatgccag ggcagttctc
     1681 acaagaaaca acttgaataa atgggtttct gatcaactaa aggatattga gatggtcatg
     1741 tacgaaagac aggaactaga aaagagtgag aatcatgaag aagcccgatt aatgaattaa
     1801 aaatctcgtt cagcgaaaga taataaggat tagataatgc ccatagaact tcatgat
//
LOCUS       DAOQVW010000331         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_81606_length_1857_cov_3.634295, whole genome shotgun sequence.
ACCESSION   DAOQVW010000331 DAOQVW010000000
VERSION     DAOQVW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81606_length_1857_cov_3.634295"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="PKK85_07085"
     CDS             complement(<1..1298)
                     /locus_tag="PKK85_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS25816.1"
                     /translation="MKIGLLHVPGALPALENFGGLPTHLVNSKGMVNGKPAHKVLDAI
                     IIPGGSIVESGSVSPFLGSEIKNMARNGSLILGICSGFQVLAEKTDIGRKSPCPILKE
                     GLGLLDVTFHPMISNDRVEAELVDDSPLTQGLKGQAITGFHCHTYGDIRGEAQPIMYS
                     LIKRADYQDDPRKVLSGVKNEEGNVVGTMVHAALDENPILRKNILQYLDATEVDIKNI
                     EKRNKVLMGRLKGEIGINTGIKASYLMDPPSNQMHPPVLMMASTASDSGKTFLTTGLV
                     GVLRKKGYRVAVLKVGPDVRDLIPSLYLNKENMEPFSSIRIGGLGWEDLPRVLEDLLA
                     RNYHLVLIEGVMSIFTGLLNEKIPYSAAEIAKAANIPVILVSSCNKGGIETAALDLAV
                     HVEMMQNLGIKARGVILNKVYHEGIAQKVLGYLKTRTGVEL"
     gene            complement(1367..>1857)
                     /locus_tag="PKK85_07090"
     CDS             complement(1367..>1857)
                     /locus_tag="PKK85_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNS25817.1"
                     /translation="EYLDLCLPTCYLINYVEFFSATRKYDGRKYGHRIEEVCGEEVLR
                     RIHMGSYISQKEYSGKYYKKALQARSLIKKEINRLLSGVDVLISPTVPKLPHKIGAEL
                     DTMEMYAYDTLTVVANLAGIPAASIPAGIVKGVPIGMQMQAKARDDLKILQLMSFIEN
                     NL"
BASE COUNT          457 a          464 c          377 g          559 t
ORIGIN      
        1 agctccaccc cagtcctggt tttcaagtaa cctaacactt tttgagctat tccctcatga
       61 taaaccttgt tcaaaatcac tccccgggct tttataccta ggttctgcat catctccacg
      121 tgcactgcca aatccagagc tgctgtttcg atacccccct tgttacagct ggaaaccaaa
      181 atcacgggaa tatttgcagc ttttgctatt tcagcggctg aataaggaat cttctcattt
      241 aacaggccag tgaagatact cataaccccc tctataagca ccagatgata gttgcgggcc
      301 aggaggtctt ccaaaaccct tggcaaatct tcccatccca gccctccaat ccgtatagag
      361 gagaagggct ccatattttc cttgttgagg tagagggatg ggatcagatc ccttacatcg
      421 ggaccaacct tcaacaccgc cacccggtag ccttttttac gcagaacccc cactaaaccc
      481 gtggttagaa atgttttccc tgaatcagaa gctgtgctgg ccatcattag tactgggggg
      541 tgcatctgat tgctgggtgg atccatcaga tagctggctt taattccagt gttgatacca
      601 atttcgccct ttaatctgcc cattaaaact ttgttcctct tttcaatatt ttttatatcc
      661 acctctgtgg cgtccaagta ttggaggatg tttttccgaa gtataggatt ttcatcaagg
      721 gcggcgtgga ccatggtccc caccacgttc ccctcctcat tcttaactcc agaaagaact
      781 tttctaggat catcttggta atctgccctt ttgatcaatg aatacattat gggttgggct
      841 tctcccctta tatcgccata ggtgtggcag tgaaatccgg ttatggcttg accctttagt
      901 ccctgggtga ggggagaatc atccactagc tctgcctcca cccggtcatt gctgatcatg
      961 ggatggaatg tgacgtccag gagccctaga ccttccttta aaatggggca gggagatttt
     1021 ctcccgatat ctgttttttc ggctaaaact tggaatcctg agcaaattcc cagtatcaaa
     1081 gagccatttc tggccatgtt ttttatttca ctacccagga atggactcac actacccgat
     1141 tctactatac tccccccagg aattataatt gcatccaata ctttatgcgc aggttttcca
     1201 ttcaccatgc ccttactatt tacaaggtgg gttggtaatc ccccaaaatt ttcaagtgcc
     1261 ggcagagcac ctggaacatg taaaagacca attttcatgt gatttttccc tattttactt
     1321 tttcaaaaaa atctgatggc tatggcctat ttccattgtg ggaaaattaa aggttgttct
     1381 ctataaatga cattaactgg agaattttaa ggtcatcccg agcttttgcc tgcatttgca
     1441 tccctattgg cactccttta acaattccgg ccggaatgct tgcggcaggg atgccagcca
     1501 ggttggcaac cacggtcaaa gtatcgtagg catacatttc catggtatcc agttcagctc
     1561 caattttatg gggtagtttt ggaacggtgg gactgatcaa tacatccacc ccggataata
     1621 aacggttaat ttctttttta ataagagaac gggcttgtaa cgccttttta taatattttc
     1681 cactgtattc tttctgactg atgtaggatc ccatgtgtat ccggcggagc acctcttctc
     1741 cacatacctc ttctatgcgg tgcccgtact tacggccatc gtacttacgg gtggctgaga
     1801 aaaattccac atagttgatc aggtagcaag tgggcaggca taaatccaga tactcga
//
LOCUS       DAOQVW010000332         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_81762_length_1855_cov_3.173889, whole genome shotgun sequence.
ACCESSION   DAOQVW010000332 DAOQVW010000000
VERSION     DAOQVW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81762_length_1855_cov_3.173889"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /gene="hypD"
                     /locus_tag="PKK85_07095"
     CDS             complement(<1..745)
                     /gene="hypD"
                     /locus_tag="PKK85_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019267883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNS25818.1"
                     /translation="MKNLSKEIIKRIEKIARPVKIMHVCGSHEHTIMQHGIRTLLPPE
                     VEVVAGPGCPVCCVPSREVDECLELARQGVTIATFGDMLRVPGSKGSLSEAKSEGADV
                     RVVYGVRNAVEMAEKLDQEVVFMSAGFETTAPTTAAELVAKPPENFSILSCHRLIPPA
                     LQFLIESGEVNLNALIEPGHVSTIIGTGPYQIFSEKYGIPQVVAGFNPLDVLMSVYMI
                     LKQIDGGKALVQNEYQRAVKEEGNLKAQKL"
     gene            1150..>1855
                     /locus_tag="PKK85_07100"
     CDS             1150..>1855
                     /locus_tag="PKK85_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNS25819.1"
                     /translation="MRAAVVGLGVEGRSAIKSLLDHGIDVYASDLDLNIAIKPQDGLE
                     IDLRFHNFEKIRSADVIVISPSLWSLNSFKAFQKSDKLISDVFSHHKSTLTIGVTGTN
                     GKTTTSLMITSILEKAGYKVLVGGNAGGGFQGYTEIILEAENKDYDVLVVEMCDMTLN
                     FCRGSFDLDLVVVTNQGNDHLDIHQSQNNYLNLIREFIMDKETILNPYDPLLVELGKS
                     SSKVHYFHKDNERKLKL"
BASE COUNT          569 a          368 c          363 g          555 t
ORIGIN      
        1 aaagtttttg ggccttaaga tttccttcct ccttcacagc ccgctggtat tcgttctgga
       61 ccaatgcttt tcccccatct atctgtttaa gaatcatgta aaccgacata agcacatcta
      121 gtggattgaa acctgctacc acctggggga tgccgtattt ttcggaaaat atttgataag
      181 ggccagttcc gattatggtg gagacgtgtc ccggctctat gagtgcattt aaatttactt
      241 caccggattc tattaaaaat tgaagggcag gaggtattaa tctatggcaa gataatattg
      301 agaagttttc gggaggttta gctactagct ctgcagctgt ggttggagct gttgtttcaa
      361 aaccagctga cataaacacc acctcctgat cgagtttctc tgccatttct accgcatttc
      421 taactccgta aacgacacgc acatcagctc cttcggattt tgcctcggat agagaacctt
      481 tactgcctgg aaccctcagc atatccccga aagtggcaat agttaccccc tgccgagcca
      541 gttccagaca ttcatcaacc tcccgggatg gtacacagca cactggacaa cctggaccag
      601 ccaccacctc cacttctggg gggagtaaag ttctaatgcc atgctgcatt atggtatgtt
      661 catgggaacc gcacacatgc atgatcttca cggggcgggc aatcttttcg atacgtttta
      721 tgatctcttt tgaaaggttc ttcatagtgg acaccataat ctacaaaagt cactgaaata
      781 aaattctgaa gtctcatgga tctgacttcg gatgtttttg aattatatat cccggaagta
      841 cataaaaact tccagcttta gattagaaat tgtcatatat ttttttacca atgaccccct
      901 tcagactctc taaaaacatt tagaaataat ttgtctccag gatttatcag caaaaaaaca
      961 aggttacaat ccacaataat catgtttatc ccaaatatta ctggattatt atccaataaa
     1021 actatttctg gctggtaaaa taactgccta gttatggtta aatactgtaa tttcaaaact
     1081 atattctata cattctctga aaatatttta gagagattaa ggtttcatca ctatattttg
     1141 gtaggttaaa tgagggcagc tgtagttggt ttaggagtag agggtagatc tgctataaaa
     1201 tctcttttag atcatgggat agatgtttat gcatcagatc tagatttaaa tatagcaatc
     1261 aaaccccagg atggtcttga aattgattta aggtttcata actttgaaaa gataagatct
     1321 gcagatgtaa tagttataag tcccagttta tggagtttga atagttttaa agcatttcaa
     1381 aaaagtgata aattaatatc agatgttttt agccatcata aatcaaccct aaccataggt
     1441 gttaccggta ccaacggtaa aaccaccacc agtctcatga tcacaagtat cctggaaaaa
     1501 gccggatata aagttctggt gggaggtaac gccggtggag gatttcaggg ttacacagaa
     1561 attatcttag aggcagaaaa caaggattat gatgttttgg tggttgaaat gtgtgatatg
     1621 accctgaatt tctgtcgggg tagttttgac ctggatctgg tggttgtaac caatcaagga
     1681 aacgatcatc tggacataca tcaatcccag aacaactacc tgaatctgat tcgggaattt
     1741 atcatggata aggagaccat actcaaccca tatgaccctt tactggtaga actcggaaaa
     1801 agttcaagta aagtccacta cttccataaa gacaatgaaa gaaagttgaa actct
//
LOCUS       DAOQVW010000333         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_81844_length_1854_cov_2.501390, whole genome shotgun sequence.
ACCESSION   DAOQVW010000333 DAOQVW010000000
VERSION     DAOQVW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81844_length_1854_cov_2.501390"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PKK85_07105"
     CDS             <1..1081
                     /locus_tag="PKK85_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25820.1"
                     /translation="RRNTAPELVKQYVYKVDRERKRDLLVELIKEKGWYQVLVFTRTL
                     APSSPELAWILTLQIFIGGILIIFLDEVVSKWGFGSGVGLFIAAGVAQQIIVGSFNPL
                     SVPGQPGVPSGALPQFFYSLTTSQPDFSLLLPVIAVVVVFFIVVYAESMRVEIPLSYG
                     GVKGARGKYPLKFIYASNMPVILTSALLLNVQLFAALFQKLGFPILGEISNGRAISGV
                     AYYLTVPGGLGVLFTDPLKVVIYGVVFIISCVIFSMLWVELSGIGPKQVAKQLHGMGM
                     QIPGFRSSRVQFERILKKYIPIITIMGGAFVGLLAFGADLTGALGGGTGVLLTVGIIY
                     KLYEEIAQEQLMDMHPMLRNFLGGE"
     gene            1085..1642
                     /locus_tag="PKK85_07110"
     CDS             1085..1642
                     /locus_tag="PKK85_07110"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNS25821.1"
                     /translation="MKVVVIAGIPGSGSTTVLENTLEDLDYVHVNYGDVMLEIAQGKG
                     LVKHRDSMRKLEPEVQKQVQMEAAQEIRKRSRENNIIVDTHCTIKTPKGFMPGLPRWV
                     LEDLQPDQFILIEADGDEILMRRVSDVTRSRDTERLEAINLHQKMNRAAAMSYAVYTG
                     ATVKIIPNHNQGLDEAVEILREALR"
BASE COUNT          462 a          494 c          489 g          409 t
ORIGIN      
        1 gcgtcgcaat actgcgcccg aactcgtcaa gcagtacgtt tacaaggtcg accgggagcg
       61 taagcgcgat cttctggtcg aactgatcaa ggaaaagggc tggtaccagg tactggtctt
      121 cacccgtacc ctggcaccat cctcaccgga actggcctgg atcctcaccc tccagatctt
      181 catcggtggg atactcatca tcttcctgga cgaggtggtg tcaaagtggg gattcggaag
      241 tggggtggga ctgttcatcg cagctggagt agcccagcag atcatagtag gatccttcaa
      301 ccccctatct gtacctggac aacccggtgt accatcaggg gccctacccc aattcttcta
      361 ctccctcacc accagccaac ccgacttctc cctcttactc ccagtgatag ctgtggtggt
      421 ggtgttcttc atcgtggtct acgccgagag tatgagggtg gagatacccc tcagctacgg
      481 aggagtgaag ggagcccggg gtaaatatcc tcttaaattc atatacgcca gtaacatgcc
      541 ggttatttta accagcgccc tgcttttaaa cgtgcagtta ttcgctgcct tgtttcagaa
      601 gctgggattc cccatcctgg gggagatatc caatggacgg gccattagtg gagtggccta
      661 ctacctcaca gtccccggtg gcctgggagt gctcttcacc gatcccttga aggtggtgat
      721 ctacggggtg gttttcatca tctcctgtgt gatattctcc atgttatggg tggaactatc
      781 tggcatcgga cctaaacagg tggctaaaca gttacatggt atgggtatgc agatacctgg
      841 attccgcagt agccgcgtcc agtttgagcg catcctcaaa aagtacatac ccatcataac
      901 tattatggga ggggccttcg tagggcttct ggccttcgga gcagacctca ccggagcatt
      961 aggtggaggt accggggtac tcctaactgt gggtatcatc tacaaactct acgaggaaat
     1021 agcccaggaa caactaatgg acatgcaccc catgctccgg aacttcttag gtggtgaata
     1081 aaagatgaaa gtcgtggtaa tagcaggaat acctggatca ggaagtacaa ccgtcttaga
     1141 aaacaccctg gaagacttgg actacgtcca tgtgaactac ggggatgtca tgctggagat
     1201 agcccagggt aagggcctgg tgaaacaccg ggacagcatg cgcaagttag aacctgaggt
     1261 gcagaaacag gtgcagatgg aagcagccca ggagatacgg aaacgctccc gggaaaacaa
     1321 catcatcgtg gacacccact gcaccataaa aacccccaag ggatttatgc ccggcttacc
     1381 ccgctgggtc ctggaggacc tgcaacccga ccagttcata ctcatcgagg ctgatggtga
     1441 tgaaatactc atgcgccggg tgtcagatgt gacccgcagc agggacaccg aacgattgga
     1501 agccataaac ctccaccaga agatgaaccg ggctgcagcc atgtcctacg ccgtgtacac
     1561 cggggccacc gtcaagatca tacccaacca caaccagggc ctggatgagg ctgtggagat
     1621 cctgcgggaa gccttaagat aagcctggga tgatacatat aaacctgatt aatgcaaact
     1681 gcttgagagg aagaaaaaaa tggtattaga attcataacc gaccccatct tcggggccct
     1741 taacacggta ctgatgccca tgattgatgc cctgggaccc atactcaccg tattcataat
     1801 cgccgccctg gtggccttct tcgacgtcca ccgtgagcat ttcgtagggt tcct
//
LOCUS       DAOQVW010000334         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_82042_length_1851_cov_3.338530, whole genome shotgun sequence.
ACCESSION   DAOQVW010000334 DAOQVW010000000
VERSION     DAOQVW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82042_length_1851_cov_3.338530"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PKK85_07115"
     CDS             complement(<1..167)
                     /locus_tag="PKK85_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNS25822.1"
                     /translation="MSNGQVPKDYDHQKETQWQEKWQQNRLYQFKPEESRPRYIIDTP
                     PPYPTGSIHMG"
     gene            300..656
                     /locus_tag="PKK85_07120"
     CDS             300..656
                     /locus_tag="PKK85_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25823.1"
                     /translation="MDLLWFYVALALALSDELHSKLFWSLFFDFYVVLAGLIQRIVGG
                     SIRMWIVHEVLESIFNFLVLSLLFLSLYIGFLAAVVHLSVDLFHEALNLNLPPLEHRA
                     LHFVVEASFFILVFSL"
     gene            702..>1851
                     /gene="pheT"
                     /locus_tag="PKK85_07125"
     CDS             702..>1851
                     /gene="pheT"
                     /locus_tag="PKK85_07125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNS25824.1"
                     /translation="MPVITFTYQNLEELLGTALDREELLELLPMMGSDVEDYDDEMVK
                     VEFFPNRPDFLSVEGVARVLRGFLGLETGLPHYQADKSGMSISADPRLEEIRPYVACC
                     VVEGISLDEEKLKQLMDFQEDLHWVLGRDRRKVAIGIHNLDVVEAPFYYRAGEPDEDS
                     FVPLECEEELTLREILQLHKKGRDYAHLLENYEYYPLIVDAHDKVLSMPPIINGELTK
                     LTLDTRNILIDVTGTDQQAVTQTLTILATSFAEAGGQIKTMDVNYPDLTLVLPDLNPQ
                     EMRVGVEDSKRIIGLNLDAQEVARALERVRMGAEAVDGDTVKVLVPPFRVDILHPVDL
                     VENVAIGYSFPDIKPELPQVATVAREDPRREFEILIREIMVGLGLVEVM"
BASE COUNT          412 a          458 c          536 g          445 t
ORIGIN      
        1 tggcccatgt ggatggatcc ggtggggtag ggtggtgggg tgtcgatgat gtagcggggc
       61 cgggactcct ctggtttgaa ctggtagagg cggttctgct gccacttttc ctgccactgg
      121 gtttcttttt gatgatcgta atctttgggg acttggccat tactcattac tttaactctc
      181 ctatttttta cttacattat aaagaagaag ccctttagta aaacatatat gctttttatc
      241 aatagttgat ataagttagt tgtagttagt atatctataa atttacggtg gataaagaga
      301 tggatttatt atggttctac gtggccctgg ccctggccct gagtgacgag ttacacagca
      361 aactattctg gagcctgttc tttgacttct acgtggtcct ggccgggctg atccagagaa
      421 tagtcggggg cagcatccgc atgtggatag tgcatgaagt tttggagagc atcttcaact
      481 tcctggtgct gtccctgctt tttttatccc tgtacatcgg atttttggcg gcagtggttc
      541 acctatccgt ggacctcttc cacgaggccc tgaacctgaa tctaccccca ctggaacacc
      601 gtgcactgca ctttgtagtg gaagcatcct tcttcatcct ggtgttctcc ctgtagacac
      661 ccagatggag tggctatgaa tgatcgtttg agttgataat aatgccagtt ataacattca
      721 cctaccagaa cctggaggaa ctcctaggaa ccgccctgga cagggaggaa ctcctggaac
      781 tactgcccat gatgggcagc gacgtggagg actacgatga tgagatggtt aaggtggaat
      841 ttttccccaa ccgtcccgac tttttatcag tggagggagt agcacgggtc ctgcgcggat
      901 tcctgggact ggaaaccgga ctaccccatt accaggctga taagtcaggg atgtccatat
      961 ccgcggatcc cagactggag gagatcaggc cctacgtggc ctgctgcgtg gtggagggga
     1021 taagccttga cgaggagaag ctgaaacagt tgatggactt ccaggaggac ctgcactggg
     1081 tgctaggtcg ggatcggcgt aaggtggcca tcgggatcca caacctggac gtagtggaag
     1141 ctcccttcta ttaccgggct ggtgaacctg atgaggactc cttcgtgcca ctggagtgcg
     1201 aggaggagtt aaccctccgg gagatactgc agctacataa gaagggccgt gactacgccc
     1261 acctcctgga gaactacgaa tactaccccc tcatagttga cgcccatgat aaggtcctgt
     1321 ccatgccccc catcatcaac ggggagctga ccaagctgac cttggacacc cggaacatct
     1381 taattgacgt aaccggaacc gaccagcagg cagtgaccca gacattaacc atcctggcca
     1441 ccagcttcgc cgaggccggg gggcagatca agaccatgga tgtgaactat cctgatctga
     1501 ccctggtgct tcctgatcta aacccccagg agatgagggt gggggtggaa gattcaaagc
     1561 ggatcatcgg cctcaacctg gatgcccagg aggtggcccg ggcactggag agggttcgca
     1621 tgggcgccga ggcagtggac ggggacacag ttaaggtgct ggttcccccc ttccgggtgg
     1681 acatcctgca cccagtggat ctggtggaga acgtggccat aggctactcc ttcccagata
     1741 taaagccgga gctaccccag gtggccactg tagcccggga ggacccccgg agggagtttg
     1801 aaatcctgat acgggagatc atggtgggcc tgggcctggt ggaggttatg a
//
LOCUS       DAOQVW010000335         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_82468_length_1845_cov_3.481006, whole genome shotgun sequence.
ACCESSION   DAOQVW010000335 DAOQVW010000000
VERSION     DAOQVW010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82468_length_1845_cov_3.481006"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            867..1382
                     /locus_tag="PKK85_07130"
     CDS             867..1382
                     /locus_tag="PKK85_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25825.1"
                     /translation="MDKERFLLPAIIIVVLLVIVAVATSLIGNTTFKTGNVAFQYPNS
                     WYKEQSIGNFDDQSLYSAVTLNRDFQSNNQTVTAYIIFQMQITTQGVLQLPSTGSITT
                     NSTNSTVGTINIGDFKATQLGNLGPEVAQKVTIIQKDNFYYTIQYICPSSALNETTEA
                     YNMILSTLQLS"
     gene            complement(1426..1788)
                     /locus_tag="PKK85_07135"
     CDS             complement(1426..1788)
                     /locus_tag="PKK85_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25826.1"
                     /translation="MLNWDYDPKRDIIFIFNFDEYDYQESIELNNMTVDLNPKRKAVA
                     LEIFGSARYLEIDPESLIDPEDFLIEIYGQNGIININASFNLNSEKGLISRILKEETG
                     IEQEFINKSILRPKILRK"
BASE COUNT          581 a          345 c          328 g          591 t
ORIGIN      
        1 caggagcacc caccagtcga attaactttg ttttttttta cattgttggt ttaacatcca
       61 tttttttgaa tctaaattag gttaaaagta ttgttatccg tctaaaacca ttgggattgg
      121 cttccagtgg ccatattatt cgcctatccg ttggtttgga attttatcga aaatcttctc
      181 cgggtccagg gaactgggat ttttatggtg gataatatgg gtggagttcc tgggggagtg
      241 gtaggaccat gcaccgtaag gtgaaggtac gtggttacat tcactttgga tcagggggat
      301 agtcatgcct tccaatacac tcaactatca agatcagtgg actaccaacc ataaaataaa
      361 aaaaaggacc agggagtgct gagaatatgc tcatagtttc atgggaggaa atacaatatg
      421 ggaagattac cactaatttc tgacttcatc tatttactta tcatcgtatt ctggaagata
      481 agagtatgga aatcacacca gagctatctc tagttgaagc ccatcccgag aaatgcagct
      541 ggtgtggaat ctgtgctgaa ccttgcccca ctggcaattt cataacgatg gttattccag
      601 caactgaacc aatgtaatta ttgtctatgt tatgtctcct tatgccctgc cggattcatt
      661 ccttcttttg caatgataaa ggcaagactt atctggctgt aatggcccac agacttgtta
      721 aaaggaaata acattttttt tcaagaaaaa gaatatttta tgggctaaaa aggtgtaata
      781 actgatatta gtgctttaaa ttcttaataa agcatttcaa caaagattat atactgtttc
      841 tgatatagtt aataataggt gatcagatgg ataaagaaag atttctttta ccggctatta
      901 tcatagtagt gttgctggtc atagttgcgg tggccacctc actaatcgga aatactacct
      961 tcaaaacagg aaatgtggcc ttccaatacc caaattcatg gtataaggaa caatctatag
     1021 gaaacttcga tgatcagtca ttatattctg cggttacact taatagggat tttcaatcta
     1081 ataaccagac tgtaactgct tatataatat ttcaaatgca aatcacaaca cagggagtct
     1141 tacaactacc cagtactggg agtataacca ctaattctac caactccact gtaggaacca
     1201 tcaacattgg cgattttaaa gcaactcaac tgggtaatct gggcccagaa gtggctcaga
     1261 aagtaactat aatacaaaaa gacaactttt attacacgat acaatacatt tgtccttcca
     1321 gcgctcttaa cgaaacaacg gaagcataca acatgatctt gagcacacta caactgtcct
     1381 aagaaaactg aatcttaata tggttggact cttccaacca tttttctatt ttcttaatat
     1441 ttttggtctg aggatagatt tattgatgaa ttcctgttct attcctgttt cttcctttaa
     1501 tattcggcta attaaccctt tttctgaatt taaattgaag gaggcattta tatttataat
     1561 cccattttgg ccatagattt ctattaagaa atcttcaggg tcaattaagg actctgggtc
     1621 aatctccaag tatcgggcag aaccaaagat ctccagggcc acagcttttc ttttgggatt
     1681 caaatccaca gtcatattat ttaactcaat tgattcctgg taatcgtact catcgaagtt
     1741 gaaaataaaa atgatatctc ttttgggatc ataatcccag tttaacattc gtttacacat
     1801 aacaatcaaa cctatcaata tgttaatgtt aaaactatat tatta
//
LOCUS       DAOQVW010000336         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_82688_length_1842_cov_2.968103, whole genome shotgun sequence.
ACCESSION   DAOQVW010000336 DAOQVW010000000
VERSION     DAOQVW010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82688_length_1842_cov_2.968103"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKK85_07140"
     CDS             complement(<1..980)
                     /locus_tag="PKK85_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNS25827.1"
                     /translation="MYFKYGLEDKPSGGKILIFGLQWLAVTIPLIIIVGNILGVLSGG
                     EINISYLQDLLLMVGLVMLVQLLYGHKLPLVVGPATVLLIAVLASQGQASGAINTSII
                     VGGVVLALVAGSGLLKYLKRLFTPRVIMVILLLIAFTLSPTILNLITIDNGVSPGINL
                     IFTMFLTLLVFTAHRVLRGLWKSTLPLWIMILGSLSYYILFGVFTPLSPNLAALSLPG
                     SFTISLAVPELTMLVSFLICFLALAINDLGSIQAVGSLLKAGEMETRVKRGITITGVG
                     NILSGLIGVIGPVNYSMSPGVIAATGNASRYTLIPAAVGLLLLACSPLSVG"
     gene            complement(1147..1287)
                     /locus_tag="PKK85_07145"
     CDS             complement(1147..1287)
                     /locus_tag="PKK85_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25828.1"
                     /translation="MVLDNSMEVLLDSFRYSLSDPDPPYSSQTVPRVMITSIWRENCP
                     DP"
     gene            complement(1409..>1842)
                     /locus_tag="PKK85_07150"
     CDS             complement(1409..>1842)
                     /locus_tag="PKK85_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNS25829.1"
                     /translation="GSYRITVAIDGCRNQDMPSGFEVVVSGGTKPMGIDAVEWARECE
                     KLGAGVILPTSMDGDGTQDGYDLEYTRAIADAVNLPIVASGGAGKLSHFYDAVEEGGA
                     TILLAASVFHFRTFTVREVKKYLKGRGLKVEDYNNEESGPR"
BASE COUNT          496 a          450 c          441 g          455 t
ORIGIN      
        1 cccactgata ggggagagca ggccaggagg agtaacccca ccgctgctgg tatcagggtg
       61 taacgggatg cgtttccagt agcagcgatc accccggggc tcatggaata attcaccgga
      121 ccaatgacac ctatgagtcc agacagaatg ttccccaccc cagtgatagt gatgccccgt
      181 ttcacccggg tttccatttc cccggccttc agaagggatc ccacggcttg gatggatccc
      241 aagtcgttga tggctagggc taaaaaacag atgagaaagg acaccagcat agtcaactca
      301 ggaactgcca aggaaatggt aaaacttcca ggaagtgata gagcagctaa attaggggaa
      361 aggggagtaa acactccaaa aagaatgtaa taagataaac tgcccaggat cattatccac
      421 aggggcagtg tagacttcca caagccacgc agcacccggt gtgctgtgaa aaccagtaaa
      481 gtcaggaaca tggtgaatat taggttgatg ccaggtgaaa ctccgttgtc gatggtgatg
      541 aggttgagga tggtggggga tagggtgaag gcgattaaca gcaggatgac catgatcacc
      601 cggggagtga acagcctttt aaggtatttt aacaatccac tgccagctac caaggccagt
      661 acaactcctc ccactataat ggaggtgttt atagcgccgc tggcctgtcc ctggctggcg
      721 aggacggcta tgaggagtac cgttgctggt cccactacca ggggcagctt gtgtccatac
      781 agtaactgta ccagcatcac caatcccacc atcaggagta gatcctgcag gtaggatatg
      841 ttgatttcac ccccgctgag gactcccagg atgttaccga cgattatgat gaggggaatg
      901 gtcacagcca gccactgcaa cccaaatata aggattttcc ctcctgatgg cttatcttcc
      961 aatccatatt taaaatacat ggcatttacc tttgttaatc atcatttata tgattttttt
     1021 acttggagtg atttacaccc ttcttttaaa taataactat tttttgttac tggtgttact
     1081 attttagttt gaaaacattg ttcaaatagt agcaggtgat gagttacgcc tttgagtaaa
     1141 tgtaggctat ggatcaggac agttttccct ccagatcgat gtgatcatca cccggggcac
     1201 tgtctgagat gagtatggag ggtctggatc agagagactg tacctgaaag agtccaggag
     1261 aacttccatg ctgttatcca gcaccaaaca ctccccatca acaataatgg agcctacctg
     1321 gatggtagtt actacatcct ctgcagattc actgaaatag aggacctata aattaacatg
     1381 tccagggagg ttagtgggcc ttcgctattt agcggggacc tgattcttca ttattatagt
     1441 cctctacctt caaacccctt cccttcaagt actttttaac ctctctcaca gtgaaggtac
     1501 ggaagtggaa caccgaagcc gccaggagaa tagtggcgcc gccttcttcc acagcatcat
     1561 agaagtgtga taacttccca gctccaccag aggccactat tggtagatta actgcatctg
     1621 caatggctct ggtatattct aagtcgtaac catcctgggt gccgtctcca tccatactgg
     1681 tgggcaggat tacaccggct cccagttttt cacattccct ggcccattcc actgcatcta
     1741 tacccatggg cttggtaccg ccagacacca ccacttcaaa tccagatggc atatcctggt
     1801 tgcggcagcc atctatggcc acggtgatac gataggagcc aa
//
LOCUS       DAOQVW010000337         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_82858_length_1840_cov_2.492997, whole genome shotgun sequence.
ACCESSION   DAOQVW010000337 DAOQVW010000000
VERSION     DAOQVW010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82858_length_1840_cov_2.492997"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PKK85_07155"
     CDS             <1..554
                     /locus_tag="PKK85_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25830.1"
                     /translation="RPREGKAKPFRYPNYLEGDVLVLELGFQDGATDYKLNNSMDWLV
                     NSAFTMVLFGVPDDRFRYVSNGHVFYNLTDALDYLDKVAAQHGQEGKIVVVYHGTARG
                     GNPNIMLGDGVPLYYQMAWLKHGRLAAYYYLLSGMLFPYYNSPYASYELFNAEQLQEL
                     YNDNGLNLKTAPYSYYELRNYNG"
     gene            716..1702
                     /gene="cysK"
                     /locus_tag="PKK85_07160"
     CDS             716..1702
                     /gene="cysK"
                     /locus_tag="PKK85_07160"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNS25831.1"
                     /translation="MVKIPELKRGIANDSTELIGNTPLVRLNKITEGVNADIVAKLES
                     FNPLGSVKDRIGVSLIEAAEEAGALKEDSVIIEPTSGNTGIALAFVAAAKGYRLILTM
                     PDTMSLERRKLLATLGAEIVLTPGANGMPGAIAKAQELLESTPNAVQPQQFQNPANPK
                     IHRETTAEELWRDTDGEIDIFVSGVGTGGTVTGVAEAIKAKKPEFKAVAVEPKTSQVL
                     AGKEKGPHKIQGIGAGFVPDVFKPDLIDEIFPVADEDAGATLLRLAREEGILAGISSG
                     AATYAALQLGKRPENEGKLIVAILPDTGERYLSVDWVFEEIYKAYPDTIPQI"
BASE COUNT          608 a          375 c          423 g          434 t
ORIGIN      
        1 agcgcccgcg cgaaggcaag gccaagccgt tccgctatcc caactacctg gagggtgatg
       61 tcctggtgct ggaactggga tttcaggatg gtgccacaga ctacaagtta aataattcca
      121 tggactggct cgtgaacagt gcgtttacca tggttctttt tggagtccct gatgacagat
      181 tcaggtacgt gtccaatgga catgtcttct acaatttgac tgatgcatta gactacctgg
      241 ataaggtagc tgcccagcac ggacaggaag gtaagatagt ggtggtttac catggaactg
      301 ccaggggagg aaacccgaat ataatgctgg gggacggagt tcccctgtac tatcagatgg
      361 cctggcttaa gcacggaaga ctggctgctt actattatct cctttctgga atgctgttcc
      421 cttactataa cagtccctat gcatcctacg aacttttcaa tgcagaacag cttcaggaac
      481 tctataacga taatggttta aatctgaaaa ccgcacctta cagctattat gagcttagaa
      541 attataatgg atagaatatt cttataattg aagttatttt ggtttaaaat atgttttcag
      601 ggagtagtaa tccacttttt tgatcgaact tttataaaaa gaaaagaata taacaatcgt
      661 tatataacta tagttataaa tattagttaa taaatagaat ataaggtgag aaaatatggt
      721 gaaaatacca gaattgaaaa ggggaatcgc caatgattcc acagaactga taggaaacac
      781 tcctctggta agacttaaca aaataacaga aggagtaaac gcagatattg tggcaaagtt
      841 agaatctttc aatccattag gaagcgtgaa ggacaggatt ggagtttctt tgattgaagc
      901 tgcagaagaa gccggagctt taaaagaaga ctccgttata atcgaaccca ccagcggaaa
      961 cactggaatt gccctggcat tcgtcgccgc agccaaagga taccggttaa tactaaccat
     1021 gccagacacc atgtccctag aaaggagaaa gttactggcg accttagggg ctgagatagt
     1081 tcttacacct ggtgcaaacg gtatgcctgg tgcgatagca aaggcacagg agcttttaga
     1141 atcaacacca aacgctgtac aaccacagca gttccagaac ccggcaaacc ccaagataca
     1201 cagggagacc actgcagaag agctctggag agacactgat ggggagattg atatcttcgt
     1261 atctggtgtg ggtaccggtg gaacagttac cggtgttgcc gaagcaataa aagcaaaaaa
     1321 accagagttt aaggcagttg ccgtagaacc taaaacctcc caggtactgg ctggaaaaga
     1381 aaaaggaccc cacaaaatac agggtatcgg tgccggattt gtcccagacg ttttcaaacc
     1441 agacttaatc gatgaaatat tcccagtagc ggatgaagac gcaggtgcaa ccttactgag
     1501 actcgctaga gaagaaggaa tactcgcagg catatcctca ggagcagcaa cctacgccgc
     1561 gcttcaactg ggtaaaagac cagaaaacga gggtaaactc atagtggcca tactcccgga
     1621 taccggagaa agatacttaa gtgtggactg ggtgtttgaa gagatctaca aggcctatcc
     1681 cgataccata ccacagatat aaatcaaatg gaatggattt aatgcataac aagaggcatt
     1741 aaattttatt ttatttttaa ccgaaagaga aaacaaaaca tctcagaaac aggctatcca
     1801 tctaaaagca gatattctaa cacaggatga ccgataaaac
//
LOCUS       DAOQVW010000338         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_83144_length_1836_cov_2.665918, whole genome shotgun sequence.
ACCESSION   DAOQVW010000338 DAOQVW010000000
VERSION     DAOQVW010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83144_length_1836_cov_2.665918"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /gene="dcd"
                     /locus_tag="PKK85_07165"
     CDS             <1..392
                     /gene="dcd"
                     /locus_tag="PKK85_07165"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNS25832.1"
                     /translation="GSPFIIHPGEFALATTYETVELPDDLVARVEGRSSMGRLGITMH
                     VTAGYIDPGFRGKITLEISNIGKMPVALYSGQRVCQIVFETMTSPSLKPYGHPDRDSK
                     YMDQEKPVTSKIKQDYEIRDRKQSRLI"
     gene            436..>1836
                     /gene="glyS"
                     /locus_tag="PKK85_07170"
     CDS             436..>1836
                     /gene="glyS"
                     /locus_tag="PKK85_07170"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNS25833.1"
                     /translation="MKNEDVMNLARKRGFLWSSFEIYSGSAGFFDYGPLGATLKNRVM
                     EKWRNYYLVREGFYEIESPTIMPEEALKASGHVDHFNDAMTQCKDCMDVYRADHVIRD
                     STGKEVEGLENQELTEILSEEKIRCPKCNGHLTHVWSYNLMFQTLIGAKGKKTGYMRP
                     ETAQGIFIPFKRLLRFFRGKLPFGVVQLGKAYRNEISPRQGMIRLREFTQAEAEIFVD
                     PHDKSHPHFHRVAQDILTLYPASCQESGAEAIQLTAEKAVMDKVVASELLTYQLCLAR
                     SFLMELGLSEDVLRFRQHMSTEMAHYAIDCWDVEVYTDRYGWIEVIGIADRTDFDLKS
                     HSNYSKEDLRVFMEYPEPLTIKKLVLKAKMGKFGPLFKGDAHMILKALEEIDAEAIQE
                     ALDDGGVYQLELQGKVHKITSDLVDFQVVEETLRGEKIYPHVIEPSYGIDRIVYSVLL
                     HSYTQEDDRSYFKLPVE"
BASE COUNT          483 a          433 c          493 g          427 t
ORIGIN      
        1 agggcagtcc cttcataatc cacccggggg aattcgccct ggccactacc tacgagaccg
       61 ttgaacttcc tgatgacctg gttgccaggg tggaggggcg ttcctccatg ggcaggctgg
      121 gtatcaccat gcacgttacg gccggttaca tagacccggg cttccggggt aagatcaccc
      181 tggagatatc caacattggt aaaatgccgg tggccctgta cagtggccag agggtgtgtc
      241 agatcgtgtt cgaaaccatg accagccctt ctttaaaacc ctacgggcat cctgaccgtg
      301 atagtaagta catggatcag gaaaaaccgg ttaccagtaa aataaagcag gattacgaga
      361 tcagagaccg gaaacagtcc cggctgatct aatcaattct atttagtgaa tcatcaaaat
      421 tcagagtgat aatatatgaa gaacgaggat gtaatgaacc tggcgcgcaa acgcggcttt
      481 ttatggtcat cattcgagat ttactccgga tctgccggat tttttgatta cggaccactg
      541 ggtgccaccc taaaaaatag ggtgatggaa aagtggagga actactactt ggtccgtgag
      601 ggattctacg agattgaatc ccccaccatc atgcccgagg aagccttgaa ggcctctgga
      661 cacgtggatc acttcaacga tgccatgact cagtgtaaag actgtatgga cgtttaccgt
      721 gccgatcatg tcatcaggga ctccacgggg aaggaagtag agggactgga gaaccaggaa
      781 ctcactgaga tcctgtcgga ggaaaagatt cgctgcccca agtgcaatgg acacctcacc
      841 catgtctgga gctacaacct catgttccag accctcatcg gtgctaaggg gaaaaaaacg
      901 ggttacatgc gacccgaaac tgctcaggga attttcatcc catttaaacg gctgttaagg
      961 ttcttccggg gtaaattacc cttcggggtg gtgcagttgg gtaaggccta ccgtaacgag
     1021 atctcccccc ggcagggtat gatacgcctc cgggagttca cccaagccga agctgagatc
     1081 tttgtggacc cccatgacaa gagtcatcct cacttccaca gggtggccca ggacatcctc
     1141 acactgtatc cagccagttg ccaggaatca ggagccgaag ccatccagtt aaccgctgaa
     1201 aaggcggtga tggataaggt agtggccagt gaactgttaa catatcagtt gtgcttggct
     1261 cggagttttt taatggaact aggtctttct gaggatgttt taaggttcag gcagcatatg
     1321 tccacggaga tggcccatta cgccattgac tgttgggatg tggaggttta cactgatcgt
     1381 tatggctgga tcgaggttat aggtatagca gatcggaccg actttgatct gaagtcccac
     1441 agtaactaca gcaaagaaga cctacgggtt ttcatggagt acccagaacc cctgaccatt
     1501 aagaaactgg tgcttaaggc taagatgggt aaattcggcc cccttttcaa gggggatgcc
     1561 catatgattt tgaaggcact ggaagagatt gatgccgagg ccatccaaga agccttagat
     1621 gatggtggag tataccagtt ggagctgcag ggaaaggtac ataaaatcac cagtgatctt
     1681 gtagacttcc aggtggtgga agagaccctg cgtggagaaa agatttatcc ccacgtgatt
     1741 gagccctcct atggtattga ccgcatcgtc tactcggtgc tgctgcacag ttacacccag
     1801 gaggatgatc gcagctactt taagctgcct gttgaa
//
LOCUS       DAOQVW010000339         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_83784_length_1827_cov_3.111738, whole genome shotgun sequence.
ACCESSION   DAOQVW010000339 DAOQVW010000000
VERSION     DAOQVW010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83784_length_1827_cov_3.111738"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PKK85_07175"
     CDS             complement(<1..770)
                     /locus_tag="PKK85_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNS25834.1"
                     /translation="MKPMSNVDVYAICQELKDILIDSRMQKAYQPTKDTVLIRFHVAG
                     QGRVDLILQAGVRVHTTHYPPPNPQLPPSFPMLLRKYLKNATVVGVRQHHFDRILELD
                     LQKEQRYRLVVELFSPGNIILLDEEDRIMLPLKRRLLRERKLASHEEYHYPTGKGINP
                     LELDYLDLENLLKESDSDVIRTLARSGLGGLYAEEVLLRAGLDKKKPSMDLDEGEVQL
                     IFNSIQSIFQPLKDQDLHPQIIQGDKEDVLPLDLEYYQ"
     gene            832..1113
                     /locus_tag="PKK85_07180"
     CDS             832..1113
                     /locus_tag="PKK85_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25835.1"
                     /translation="MEVEAKVVTVHIIAALVAAFLSFLLSTGAISGVGTNEALATLVG
                     LVILYLSGQLSEKLFGKEEVGGFKGWLWSGIVPFLLFWYMLWTMFMNLL"
     gene            complement(1137..>1827)
                     /locus_tag="PKK85_07185"
     CDS             complement(1137..>1827)
                     /locus_tag="PKK85_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HNS25836.1"
                     /translation="LSLIKAAEGRDGVDNVVLVDCHNSFNGESGRVLAGNKEVFQLLC
                     AVENIKRPEETGNVRMGWAHDPLSHLDRKDGVGQSGVKAMVVEVDSQKTGYILLDSNN
                     MIQGFREELIEEVLKLGLDEVEVMTTDTHYVNTLSGGHNPLGVKKKDEITAGVLGCVQ
                     KALGDLESVKVGCKTSRLSGINTLGPTQATELVTTISSIVAVSRIFAPLVLIVALLFA
                     FVWIFYGALHL"
BASE COUNT          394 a          497 c          464 g          472 t
ORIGIN      
        1 tcctggtagt actccaggtc cagggggagc acgtcctcct tatccccctg gatgatctgg
       61 gggtgcaggt cctggtcctt caggggctgg aatatactct ggatggagtt gaatatcagc
      121 tgcacttccc cctcatcaag gtccatgctg ggcttcttct tatccaaccc ggcccttagt
      181 aggacttcct cggcgtacag tccccccagg ccactgcggg ccagggtgcg gatcacgtcg
      241 ctatcagact ccttaaggag attctccaga tccaggtaat ctagttccag ggggttgata
      301 cccttaccag ttgggtagtg gtactcttca tgggaggcca gcttcctctc ccgtaggagt
      361 ctccttttca gggggagcat gatccgatcc tcctcatcca aaagtattat attgcctggg
      421 gagaaaagtt caacaaccag ccggtatcgc tgttctttct ggaggtccaa ctccaggata
      481 cggtcaaagt ggtgctgtcg gacccctacc acggtggcgt tcttcaggta cttgcgcagt
      541 aacatgggga aggagggtgg tagctggggg tttgggggtg gatagtgggt ggtgtgcacc
      601 cgcaccccgg cctgcaggat caggtccacc cggccctggc ccgccacgtg gaatcgtatg
      661 agaacagtat ccttagtggg ctggtaagct ttttgcatgc gactatcaat caggatatcc
      721 tttaattcct ggcatattgc ataaacatca acgttggaca tgggtttcat ggtcaaatac
      781 accctcatct atattatatg aaaagttata gttataaatc ggaggtaaaa aatggaagta
      841 gaagctaaag tagttactgt acacattatc gcagccctag tggcagcttt cttgtcattc
      901 ctattatcaa ctggagccat atccggtgtg ggcaccaacg aggccctggc tacactggtg
      961 gggctggtga tcctctacct ctccggccag ttatccgaga aactctttgg gaaagaagaa
     1021 gtaggtggct tcaagggctg gttgtggagt ggaatcgtac ccttcctact gttctggtac
     1081 atgctgtgga ccatgttcat gaacttactc tgatccacct tctttttcag gtaccctcac
     1141 aggtgcaggg cgccgtagaa aatccataca aaggcgaata acagggccac aatcagtacc
     1201 agtggtgcga agatgcggct caccgccaca atggagctga tggtggttac cagttccgtg
     1261 gcctgggtgg ggcccagggt gttaattccc gagaggcgac tggttttaca gcccaccttc
     1321 acggattcca ggtcacctag agctttctgg acgcatccca gcacacctgc agttatttca
     1381 tcctttttct taacacccag ggggttgtgg ccaccggaga gggtgttaac gtagtgggtg
     1441 tccgtggtca tgacctccac ctcgtccaat cccagcttca gcacttcttc tattagctct
     1501 tcccggaagc cctggatcat gttgttgctg tccaggagta tgtagcctgt tttctggcta
     1561 tccacctcca ccaccatggc cttaactccg ctctgcccca caccatcctt acggtccaag
     1621 tgacttaggg ggtcatgagc ccaacccatc cgcacattac cagtctcttc aggccttttt
     1681 atattctcaa cggcacataa aagctggaaa acctctttat tacccgctaa aacccggccg
     1741 gattctccgt tgaagctgtt gtgacagtcc accagcacca cattatccac tccgtcccgg
     1801 ccctctgctg ccttgatcag tgataaa
//
LOCUS       DAOQVW010000340         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_84181_length_1822_cov_6.029428, whole genome shotgun sequence.
ACCESSION   DAOQVW010000340 DAOQVW010000000
VERSION     DAOQVW010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84181_length_1822_cov_6.029428"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKK85_07190"
     CDS             <1..248
                     /locus_tag="PKK85_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25837.1"
                     /translation="KELRSLHEITNQKDWTTKVRELTRTEEDEISNEIQALELQVEEL
                     SDELDTIRIRIEDTKWELALKLEFYRGSDELIKIEGK"
     gene            255..743
                     /locus_tag="PKK85_07195"
     CDS             255..743
                     /locus_tag="PKK85_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25838.1"
                     /translation="MVFETLKKEKHELEETPDIEGYDEFLLNADMEPDMTFTGNPWMG
                     VPYKFDLDYDGNKSTNYRVNFWIVNKENQEVLKTQLKLKSLEDEARFWENSLGFDLID
                     SIDQLHGGEGNHNILTISLKELSEYLNKQNTMTIRVIEHSAKIKGEITTWNSLKVTEI
                     GG"
     gene            749..1459
                     /locus_tag="PKK85_07200"
     CDS             749..1459
                     /locus_tag="PKK85_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HNS25839.1"
                     /translation="MTTEEPTKGTTEFMEDLIQLQSEIHNPSNTATNPFLKSKYAPLN
                     EILNMVRPLCNKHGFFLFQDISGDGEFVTVETNILHRTGHSIRSGELEIKPDKKGIQG
                     AGAAITYMRRYQLLSLLGIAGEDDNDGEGSKDTVNEPAKTSKTVKQPKSAKASKTTKK
                     VPKSTQKSSDESEVLPDYNTVAKESAVFRIIQEEIKTRDLVLCDDNIIEIGSDLVGQR
                     TITPGDFTVVRKKLGLEV"
     gene            1465..1710
                     /locus_tag="PKK85_07205"
     CDS             1465..1710
                     /locus_tag="PKK85_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25840.1"
                     /translation="MFNKNLFASDLESAICRVCTFHLKPGHPIGETIQARVTSARMSL
                     GDNLYLFVEYEGEKFLLKSRDFTTFKIPSQAGVEESL"
BASE COUNT          646 a          406 c          397 g          373 t
ORIGIN      
        1 acaaagaact ccgaagcctc catgaaatca ccaaccaaaa agactggacc accaaagtcc
       61 gggaactaac caggactgaa gaggatgaaa tatccaatga aatccaagcc ctggaactac
      121 aagtcgaaga actaagtgac gaacttgata cgatacggat ccgcatcgaa gacactaaat
      181 gggagttagc attgaaactg gagttctacc gtggaagcga tgaactaata aaaatagaag
      241 gaaagtgatt ataaatggta tttgaaacat taaaaaaaga aaaacacgaa ttagaagaaa
      301 cacccgacat agaaggctac gatgaattcc tactcaacgc tgatatggaa ccagacatga
      361 ccttcaccgg taatccatgg atgggagtac cgtataaatt cgacctggac tacgatggaa
      421 acaaatcaac gaattaccgg gttaacttct ggatagtcaa caaagaaaac caggaagtac
      481 tcaaaaccca actcaaacta aaaagcttag aggatgaggc ccggttctgg gagaacagcc
      541 tcggcttcga cctaatagac agcatagacc aactccacgg aggagagggc aaccataaca
      601 tcctcaccat cagcctaaaa gaactcagcg aatacctgaa caaacaaaac acgatgacca
      661 tccgggtcat agaacacagt gccaaaatca agggagaaat caccacctgg aacagcctaa
      721 aagtcacaga gatagggggc taaaggcaat gacaactgaa gaacccacta agggaacgac
      781 agagtttatg gaagatttaa tccagttaca aagtgagatc cataatccat ctaacacagc
      841 caccaatcca tttttaaaaa gcaaatacgc ccccctaaat gaaatcctca acatggtcag
      901 accactatgt aataaacatg gatttttttt atttcaagat attagtggag atggagaatt
      961 tgtcactgtt gaaacgaata tcctccacag aacaggacat agtattagat ccggagagtt
     1021 agagattaag cctgataaga agggcatcca gggagcgggg gcagccatca catacatgag
     1081 aagataccaa ctcctcagcc tcctcggtat agctggggaa gatgacaacg atggtgaagg
     1141 aagtaaggat actgttaatg aaccagctaa aacaagtaag acagttaaac aacctaaaag
     1201 tgccaaggca tctaagacaa ctaagaaggt tcccaaaagc acccagaaat ccagtgatga
     1261 gagtgaagtc ctccctgact acaataccgt agctaaggag agtgcggtct tccggatcat
     1321 tcaagaagaa atcaaaacac gtgacttggt actttgtgat gataacataa tcgaaatcgg
     1381 aagcgaccta gtcggccagc gaaccattac ccctggcgac ttcactgtgg tcaggaagaa
     1441 actcggctta gaggtgtaac ttccatgttc aacaaaaatt tatttgcaag tgacctggaa
     1501 tcagcaatct gcagggtatg caccttccac ctgaaaccag gacaccccat cggagaaacc
     1561 atccaggcca gggtcacatc cgctaggatg agcctcggtg ataatttgta tttgtttgtg
     1621 gagtatgagg gtgagaagtt cttgttgaag agtagggatt tcaccacttt caagataccc
     1681 agccaggcag gtgtggagga atctctatga ccatccaatc catcctagac cccgttatcc
     1741 tttttttgat ggccctggtt atcatcatca ctgtgggtgt cctggccgta gaaatccgat
     1801 acctggacta acaccatgac aa
//
LOCUS       DAOQVW010000341         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_84824_length_1814_cov_3.247300, whole genome shotgun sequence.
ACCESSION   DAOQVW010000341 DAOQVW010000000
VERSION     DAOQVW010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84824_length_1814_cov_3.247300"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PKK85_07210"
     CDS             <1..903
                     /locus_tag="PKK85_07210"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNS25841.1"
                     /translation="EELQDHVQDVNIFARVVPEEKLRLVEAFKARGDTVAMTGDGVND
                     APALKSAQIGISMGGRGTDVAREASALVLLDDDFSSIVLAVKMGRRIFDNLKKATSYI
                     FAVHVPIVGMSLLPVIFGWPLVLFAMQIVILELIIDPACSVAFEAEPAEEDVMKRPPR
                     SKKDPLFEPRTIGISILQGVVVLLIVLAVFVVAFYGGRGEDTSRALAFTTMLFGNLAL
                     ILSNRSWSKTIMETLHSPNPALWWIIALAVIILGLVLYLPSLQSLFQFGVLSATDLLL
                     CLLAGGTSVLWFEALKAAKFRLQP"
     gene            961..1791
                     /locus_tag="PKK85_07215"
     CDS             961..1791
                     /locus_tag="PKK85_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25842.1"
                     /translation="MAESIKSRVRKKCQEMGIPLVGFAPVERWENPPEELPHHFEEWI
                     PPEFWPQSIYPEAKTVIVIGLPVQLPILETAPSIFYHELYHTINQLLDEEAYLLSNYL
                     TSQGHPSIYLPRDGYGDIEVLLKKPLAFFSHKHAAYLAGLGSIGWNNVLLTPEYGPRV
                     RFTSIFTTASLDGDPIIGYDLCTRCLLCARTCPVHALATRKDEHFPPPMEKINCANRS
                     RKLRKKYQSPCGICIKVCPVGMDRVVFGREDTSLYSQEEGFEKYHQAWAHVKNYGSKK
                     "
BASE COUNT          392 a          525 c          479 g          418 t
ORIGIN      
        1 gaagagctcc aggaccatgt ccaggatgtt aacatctttg cccgggtagt tccggaggaa
       61 aagctgcgcc tggtggaggc cttcaaagcc aggggtgata ctgtggccat gactggagat
      121 ggggtgaacg atgccccggc acttaaatcc gcccagatcg gtatcagtat gggtggccgg
      181 gggacggatg tggcccggga ggcctccgcc ctggtgctcc tggatgatga tttttcctcc
      241 attgtcttgg cggttaagat gggccggcgc atcttcgaca acctgaagaa ggccacctcc
      301 tacatcttcg cggtgcacgt gcccatcgtg ggcatgtccc ttttaccggt gatctttggc
      361 tggcccctgg tcctctttgc catgcagatc gtcatcctgg aactcatcat cgaccctgcc
      421 tgttcagttg ccttcgaggc agagcccgca gaggaggatg tgatgaagcg gccaccccgc
      481 agtaagaaag accccctctt cgaaccccga accataggga tcagcatcct acagggggtg
      541 gtggtgctcc tcatagtcct ggccgtcttt gtggtggcct tctatggggg ccggggcgag
      601 gacacctccc gggctctggc cttcaccacc atgcttttcg gtaacctggc cctgatcctc
      661 tccaaccgtt cctggagtaa aaccatcatg gagaccctgc actcacctaa cccggccttg
      721 tggtggatca tagccctggc agttattata ctgggactgg tactctacct gccatcactg
      781 cagagcctgt tccagttcgg ggtgctctca gcaactgatc tcctgctctg cctcctggct
      841 ggtggcacta gtgtgctgtg gttcgaggca ctgaaggccg ccaagttccg tctgcagccc
      901 tgacagcctg gaccaggcag ggaataataa atattaatcc ccagacctaa actagaaaac
      961 atggctgaat ccataaaaag cagggtcaga aaaaagtgcc aggaaatggg catacccctg
     1021 gtgggctttg caccggtgga aaggtgggaa aatcccccgg aggagctgcc ccaccacttt
     1081 gaggagtgga tacccccaga attctggccc cagtccatct accccgaggc taaaacagtt
     1141 attgtaattg gactgccagt gcagctcccc atcctggaga ctgcgccctc catcttttac
     1201 cacgaactgt accacaccat caaccagcta ttggatgaag aggcctacct cttatcaaat
     1261 tatcttacca gccaaggaca tccttctatt tacctacccc gggatgggta tggtgatatt
     1321 gaagtgctcc tgaaaaagcc cctggccttt ttctcccaca aacacgctgc atacctggct
     1381 ggtctgggat ccattggttg gaataatgtg cttttaactc cagagtatgg accccgggtt
     1441 cgtttcacct ccatcttcac caccgcctcc ctggatggtg accccataat tggttatgac
     1501 ctgtgcaccc ggtgcctcct ctgtgctaga acttgccctg ttcatgccct ggccacccgt
     1561 aaggatgagc acttcccccc acccatggag aagatcaact gtgccaaccg gagcaggaaa
     1621 ctccgtaaaa agtatcagtc cccctgcggt atctgcatca aggtctgccc tgtggggatg
     1681 gatcgggtgg tgtttggccg ggaggacacc tccctctact cccaggagga gggtttcgag
     1741 aagtatcacc aggcctgggc gcacgttaaa aattatggga gtaaaaaata atcctatgga
     1801 ctatgaattc gcag
//
LOCUS       DAOQVW010000342         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_85980_length_1799_cov_3.435780, whole genome shotgun sequence.
ACCESSION   DAOQVW010000342 DAOQVW010000000
VERSION     DAOQVW010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85980_length_1799_cov_3.435780"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..907)
                     /locus_tag="PKK85_07220"
     CDS             complement(71..907)
                     /locus_tag="PKK85_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNS25843.1"
                     /translation="MKEKLLQSSYKPIIIIPLIVTILSLVLVAVNGLPGSIDLDGGTT
                     TVLNLEKPVSQSELENIISTGMGNPVVDVVSITGTQAQVEISGNVDVTKLSDVLEGTA
                     TIESYRSVGALLSQEALTQVYYALAFAFLFMSITVFIIFRSMVPSLAVIIAAASDIII
                     AIGGMSLLGIPLSMASVGGLLMLIGYSVDTDILLTTRVLKDKKGTVTERAMKAMGTGL
                     TMTIAAIGAMVALYLVVALFIPAASTLADIAAVLIIGLVADIMTTWVMNLGILRWYME
                     AR"
     gene            complement(923..1471)
                     /locus_tag="PKK85_07225"
     CDS             complement(923..1471)
                     /locus_tag="PKK85_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNS25844.1"
                     /translation="MRGIMMKTVILFYSRTRKTAMVAKTLAKEVGADLVEVTDLTDRT
                     GPLNYFKSALDAVRENKTNIKPEKIDLSPYGLIYIGGPTWASKPAPAIITLIDQCKLQ
                     GKDVIPFTTVGRNTAGQATFATMGNRAARQVINRMREKIEARGGRLVNSFIIGTGGLS
                     ELEIEEEVKNAVQELDLPIYGF"
     gene            complement(1631..>1799)
                     /locus_tag="PKK85_07230"
     CDS             complement(1631..>1799)
                     /locus_tag="PKK85_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNS25845.1"
                     /translation="YCHVGAFQSDENGRLVVVSAIDNLVKGASGVAVQNMNLMCGFPE
                     TLSLESIGLHP"
BASE COUNT          429 a          467 c          440 g          463 t
ORIGIN      
        1 tatgctccct gccaccagaa ttatgaggag aataaccctc cagtccttga taaactcttt
       61 gaaattcatt ttagcgtgcc tccatgtacc atcggagtat tcccaggttc atgacccagg
      121 tggtcataat gtccgccacc agtccaatga tgagtaccgc ggcgatatcg gccagggtgg
      181 aagccgcagg tatgaagagg gccaccacca ggtacagggc taccatggca cctatggctg
      241 ctatggtcat ggttaaacct gttcccatgg ccttcatagc cctttctgtg acggttccct
      301 ttttatcctt caagacccgg gtggttaata ggatgtcggt gtccacactg taaccaatga
      361 gcatcagcag tccacccacc gaggccatgg atagggggat gcccaagagg ctcatcccac
      421 ctatggcgat tatgatatcc gaggcagctg caattataac tgccagggat ggtaccatgc
      481 tacggaagat gatgaatacg gtgatactca taaagaggaa ggcgaaggcc agggcgtagt
      541 acacctgggt cagggcctct tgacttaaaa gagcacccac ggatcggtaa ctttctatgg
      601 tggctgttcc ctccagtacg tcactgagtt tcgtcacatc cacgttccct gatatctcca
      661 cctgggcctg ggtgccggtg atggacacta catccaccac tgggtttccc attccagtgg
      721 atatgatgtt ttccagttca ctctgactca ccggcttttc cagatttaaa acagtggttg
      781 ttccaccatc caagtctata cttcctggaa gtccgttaac tgccaccagc accaggctta
      841 gaatggttac aataaggggg atgatgatga ttggcttgta agatgattgt agtagtttct
      901 ctttcatggc attcaccaag ttctaaaatc cgtagatggg aaggtccagt tcctggaccg
      961 cgtttttaac ttcctcttca atttccaatt cagataggcc accagtacct atgatgaagg
     1021 agttcaccag gcgtccgccc cgggcttcga ttttctcccg catacgattt ataacctggc
     1081 gggcggcccg gttacccatg gtggcgaagg tggcttggcc agcagtgtta cgccccactg
     1141 tggtgaaggg tatcacatcc ttgccctgga gcttgcactg gtctatgagg gtgatgatgg
     1201 cgggggcggg cttggaagcc caggtggggc ctccgatgta tatcaaacca taaggggata
     1261 gatcgatctt ttcaggtttt atattggttt tattctctct aacagcatct agggctgatt
     1321 taaaataatt caagggaccc gtacggtcgg tgaggtcagt aacctccact aggtcagctc
     1381 caacttcctt ggccagtgtt ttagctacca tcgcagtttt ccgggtccgg gagtaaaaca
     1441 aaatcacagt cttcatcata atgcctcgca actatccccc aggtagaaaa ccagaacaac
     1501 cgccattacc cgggacttag aggttctaca ctggctcata atttttatac tctaccatat
     1561 aaggtttagg cttaaagctg gatctgcatc taaaacccca gctataccat taaaggtaag
     1621 acatccagtt ctaggggtgt aagcctatgc tctccaggga taaggtctca gggaagccgc
     1681 acatcaggtt catgttctgc accgccactc cagatgctcc cttgaccagg ttgtcaatgg
     1741 ctgacaccac caccaggcgt ccgttttcat cggactggaa tgccccgacg tggcagtag
//
LOCUS       DAOQVW010000343         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_86051_length_1798_cov_3.660356, whole genome shotgun sequence.
ACCESSION   DAOQVW010000343 DAOQVW010000000
VERSION     DAOQVW010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86051_length_1798_cov_3.660356"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..865
                     /locus_tag="PKK85_07235"
     CDS             113..865
                     /locus_tag="PKK85_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNS25846.1"
                     /translation="MEEKNKEKEYWQMLDRQKKLISTEEQLLLKKSQILVVGCGGIGG
                     AVAEMLARMGIGKIRVVDKDHFEISNLNRQLMSDTTNIGRFKAEVTRENLLKISPQLN
                     VEAFSQELTTENVAQLLEGCSVVIDALDNLFSRIILSREALKRNIPFIHGAIHGTKGQ
                     ITTFTSRTISYEKMFKLPSHGKDLTPELMEELEKLSQEIPPSTSSVPNIVGCIQAFEA
                     MKIIISRGSPIFAPDMLVFDLFKKNPFSLVKL"
     gene            978..>1798
                     /locus_tag="PKK85_07240"
     CDS             978..>1798
                     /locus_tag="PKK85_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNS25847.1"
                     /translation="MEDNVGKVIQEIERCGIKFVRLQFVDIHGTPKNMAVPLAKPDDF
                     EEILKNGLLFDGSSVAGFVNINDSDLIIKPDPDTFSPLPWRPEEKGVCRFICDICRPD
                     GTPFEGDPRYILRRTLEKAEKKGYEYNVGPEPEFFIVEKDENGKIRPHDSGEYFDVEP
                     MDQGTDMRRELVLGLEELKFDVETSHHEVAPGQHEISFKFDDALKTADAVITFKQAIK
                     AIVDKMGYLVTFMPKPFFGENGSGMHCHQSLFQDGENLFYDPEGKYELSQEALYF"
BASE COUNT          595 a          352 c          389 g          462 t
ORIGIN      
        1 tatttttgat atactataat gtaagagttc atataagggt tgacatggaa ggcattagta
       61 gttacaccag ttaacagtaa tttattagaa tactaccatc gagtgtgagg aaatggaaga
      121 aaaaaacaag gaaaaggaat actggcagat gctggatcgc cagaagaaat taatcagtac
      181 cgaagaacag cttctgttga aaaaatctca aatactggtg gtgggttgcg gtggtattgg
      241 aggagcagtt gcagagatgc tggcccgcat gggtatcggg aaaatcaggg tggtagataa
      301 ggatcatttc gaaatttcca atttaaatcg acaattaatg agtgacacaa ccaatattgg
      361 acgctttaaa gcagaagtaa ccagagaaaa cctgcttaaa atttccccac aattgaatgt
      421 ggaagcattt tcccaggaat taacaacaga aaatgtggca cagcttttgg aaggctgcag
      481 tgttgtgatt gacgctctag acaacctttt cagcagaata atacttagta gagaagcttt
      541 aaaaagaaat attcccttta tacatggagc aattcacgga accaaaggcc agataaccac
      601 attcacttcc agaacaattt cttatgagaa aatgttcaaa ttaccatccc atggaaaaga
      661 tttaacccca gagctcatgg aagaattgga aaaactaagc caagagatcc ctcccagcac
      721 atcttcagtg cccaatatag tgggatgcat acaagccttt gaagccatga aaataattat
      781 ttcccggggt tcaccaattt ttgccccgga tatgctggta ttcgaccttt ttaagaaaaa
      841 tcctttttca ctggttaaat tgtagatttt ttagccgttt ttttctccat ttatcagttc
      901 agtatattcc accacataat ttatctacca gtaaaaggaa acatctttcc ggtgtaattt
      961 tcagggaggt ttactcaatg gaagataatg ttggaaaggt aatccaagaa atcgaaagat
     1021 gcggcattaa atttgtacgg ctacaatttg tagacataca cggaactccc aaaaacatgg
     1081 cggttccact ggctaaacca gacgattttg aagagatatt gaaaaacgga ctcctatttg
     1141 acggttcttc agtggctggt ttcgtgaaca tcaacgacag tgatctcatt ataaaacctg
     1201 accctgacac cttctctccc cttccctgga gaccggaaga aaaaggcgtt tgcagattta
     1261 tatgcgacat ctgccggcca gatggaaccc ccttcgaagg ggacccgcgt tacatattaa
     1321 gaagaaccct ggaaaaagcg gaaaaaaaag gttacgagta caatgtggga cctgaaccag
     1381 agtttttcat tgtagaaaaa gatgaaaatg gaaaaatacg tccccacgat agcggagagt
     1441 acttcgacgt ggaaccgatg gatcagggca cagacatgcg ccgcgaactg gttctgggat
     1501 tggaggaact gaagtttgat gtggaaacca gccaccatga ggtggctcct ggtcaacacg
     1561 aaataagttt caagtttgat gatgccttaa aaactgctga tgcagttata actttcaaac
     1621 aagccataaa ggccatcgta gataagatgg gctatctggt aaccttcatg cccaaaccct
     1681 tctttggaga aaatggtagc ggtatgcact gccaccagag cctcttccaa gatggtgaaa
     1741 acctatttta tgatccagaa ggaaaatacg aactttccca ggaagccctg tacttcat
//
LOCUS       DAOQVW010000344         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_86052_length_1798_cov_3.653471, whole genome shotgun sequence.
ACCESSION   DAOQVW010000344 DAOQVW010000000
VERSION     DAOQVW010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86052_length_1798_cov_3.653471"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PKK85_07245"
     CDS             <1..438
                     /locus_tag="PKK85_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HNS25848.1"
                     /translation="QDIMRATSFLTEDAKYMPAGYREDYIQHFSKAFFGRIADLKKDE
                     GHYQGGVDAIRLQAFLDVLERQRSEAKSEDELCFLKIARVIAIYTTFIREEPVHPVGT
                     KFPGGFTLRYEGGKYLCPVKDHQLKNPSALCRFCVSVQDKSVE"
     gene            complement(1138..1755)
                     /locus_tag="PKK85_07250"
     CDS             complement(1138..1755)
                     /locus_tag="PKK85_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25849.1"
                     /translation="MSQYGLDQKNINLDEITENSLKNLALFEVIKTGVLSKDDVTRYN
                     CYQILLRLSEEHPDFLYLHWDYFYKMLKSNNNYHQNIAINILANLSMVDVENRFEDIF
                     EDYYHILAGERTITASHVALNSSTIYINKPELRSIIIDQLLNIDNIHRGKQKELVKAY
                     AIESLLKIYPHEEDKKRIDDFVKSQLKSSSPKTRNMAACFMEKCL"
BASE COUNT          576 a          341 c          323 g          558 t
ORIGIN      
        1 caggacatca tgcgggccac ctcattttta acagaagatg ccaagtacat gcctgcaggt
       61 taccgtgaag attacattca acacttcagc aaagcctttt ttggtcgaat tgctgacctt
      121 aaaaaggatg aaggtcatta ccagggcggg gtggatgcca tccgtctgca agcctttctg
      181 gatgttctgg aaaggcagcg tagtgaagca aaaagtgaag atgaactctg ttttctaaaa
      241 attgcccgag tgattgccat ctacaccacc tttatccggg aagaacctgt gcatccagtg
      301 gggactaaat ttcctggtgg tttcactctg agatacgagg gaggaaagta tctgtgtcca
      361 gtcaaggacc atcagctgaa aaatcccagt gccctatgcc ggttctgcgt ttcagtacag
      421 gacaaatctg ttgaatagat ttagaaagtc ttcatggata ataataatct tcctagagta
      481 attctaagtt catttcatta atctgatcaa gttccaagat cacgtcttta caaaaattcc
      541 cacaaaaacc agtgaaaagg tggaaattaa aagtacagac tcataccacc cgttaaaccc
      601 tttgatgagt ggatcattcc ctatataata ataaaacaca gataaacctg gtaaagtatc
      661 agttcagtaa ccactggagc cagtgcattt aaaaggtaag ataaaagtcc cagtctaagt
      721 gcttttccca tactgagttc ctgatcccac tgtagggcaa gtaaaaaaaa tggggtcaaa
      781 gctccaaaaa atgttccaga caagatcaaa taccgataaa tcccagagac gcatcggata
      841 tggtcaagaa gaaaactgat ggtggtagtc ttatcagccc cgtttggtcc gagataacca
      901 aaaaaacttc gacttgatga atttcaaaat tgagttcaac tactccttgt tctttatttt
      961 agagattgct cagattcgga gaccataact ggactgctaa tgagttactc ctaataaatt
     1021 aaactaatag gtacgtgtta acataattcc atgtataaaa aaattatttc taataattaa
     1081 aagccattca aaacaaaaaa agttacagca tccgacttca aatcaactaa tattttgtta
     1141 aaggcatttt tccataaaac aagccgccat attacgggtt ttaggactgg aactttttaa
     1201 ctgtgatttc acaaagtcat cgattctttt tttatcttcc tcatgaggat atatttttag
     1261 caatgattca atggcataag ccttcaccag ttctttttgt tttcccctgt gaatattatc
     1321 aatgttaagt agctgatcaa taattatgct tcgtaattct ggcttgttaa tgtagatggt
     1381 agatgaatta agggccacat gagatgcagt tattgtccgt tctccagcga gaatgtggta
     1441 ataatcttca aaaatatctt cgaatctatt ttcaacatcc accatgctta aattagccaa
     1501 gatattaatg gctatgttct gatggtaatt gttgttactc ttcagcattt tgtaaaaata
     1561 atcccagtga agatataaaa aatccggatg ttcttcgctc aaacgcagta aaatctggta
     1621 acagttatat cgggttacat catctttaga caggacacct gtttttatta cttcaaataa
     1681 agccaaattt ttcagggaat tttctgttat ttcatccaag ttgatatttt tctggtctaa
     1741 gccatactgt gacatttttt actccagttc gtgttttttt atattagttc ttaaaaat
//
LOCUS       DAOQVW010000345         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_86157_length_1797_cov_2.893226, whole genome shotgun sequence.
ACCESSION   DAOQVW010000345 DAOQVW010000000
VERSION     DAOQVW010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86157_length_1797_cov_2.893226"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1797
                     /locus_tag="PKK85_07255"
     CDS             <1..>1797
                     /locus_tag="PKK85_07255"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS25850.1"
                     /translation="GKRYIVIPVIRLGNVIITPYPIRPGFAWELYEENGTFSTDQGEI
                     PPHHQYIALYKWLEEAFNANVMIHMGTHGTMEWLPGRHVGLQEDDWPFQLSNIPNINP
                     YIVSNPGEGLVAKDRANALIIDHMTPAMVRSGLYGDLIVMHDLIHQYQNALNVGNYQI
                     LPALATQITVKVNELGLESQATGQNFEEWLDKIHLLLHEIEKDIIPLGLHSLGKVLTG
                     DELVEEVFTIVSSMTDIMDHMKTELYPRITVSYYDMHKVTLYTDELDAIDNQIRDYIE
                     RIVNGDEPTSLSVTSTDLLDDLQYCKQIINKIRANQEWESLLNALSGGFVTAGLGADP
                     AYADVLPTGKNFYSANPKKMPTKAAWETAKIVVDQLIVDYYQKHGNFPETIGMVMWGT
                     ELLRTDGIAIGEFLYLLGVQPIWNSNGDVQSTPTLIPLEDLTVTIDGVTMKRPRIDVF
                     TTAVTGNEIWINLMNNAVKLAANAPGETDKENNIKKHLLENDSLDRVFGLRGLVLEGT
                     GVCDLVPNTSKWSTSDDLASVYLSRVSYAWRSTDNGIQISQNRNTFQYLLRNMDMVTQ
                     TIDSTWRLLDTDDYYDWFGGMFLASTSLGGNPD"
BASE COUNT          586 a          355 c          389 g          467 t
ORIGIN      
        1 gggaaaagat acattgtaat cccggtcatc cgactgggaa atgtgatcat aacaccctat
       61 ccaatcagac caggttttgc atgggaacta tatgaagaaa acggcacttt ctccacagat
      121 cagggggaaa taccaccaca ccaccagtac atagcattgt acaaatggct ggaagaagcc
      181 ttcaatgcca atgtaatgat acacatgggg acccacggaa ccatggaatg gctcccagga
      241 cggcacgtag ggttacagga agatgattgg ccattccaat tatctaacat acctaacatc
      301 aatccctaca tagtttccaa ccctggtgaa ggtttggtgg ccaaggatag ggctaacgca
      361 ctaatcatag accacatgac accagccatg gtccgttctg gtttgtacgg tgatttgata
      421 gttatgcatg acttgataca ccagtatcaa aacgctttga acgtgggtaa ctaccagatt
      481 ttaccagcat tagcaacaca gataacagta aaagttaatg aattaggcct agaatcacag
      541 gcaacaggac aaaactttga agaatggctg gataaaatcc atctattgtt acatgaaatc
      601 gaaaaggata tcatacccct cggtttgcat agcctcggga aagttctaac tggtgatgaa
      661 ctagtggaag aagttttcac aatagtatca tccatgactg atatcatgga tcacatgaaa
      721 actgaacttt atccaagaat aacagttagc tattatgaca tgcacaaagt aaccttgtat
      781 acagatgagt tagatgccat agataaccag attagggatt acatagaacg aatagtgaat
      841 ggtgatgaac caaccagttt aagtgtcacc agcactgatt tactggatga cttacaatat
      901 tgcaaacaaa tcattaacaa aataagggca aaccaggaat gggagagtct tttaaacgcc
      961 cttagtggtg gatttgtcac agctggttta ggggctgatc cggcctatgc tgatgtttta
     1021 ccaactggta aaaatttcta cagtgctaat cctaagaaaa tgcccactaa ggcagcatgg
     1081 gaaacagcaa aaatagtagt agatcaacta atagttgact actaccaaaa acatggaaac
     1141 ttcccagaaa ccattggaat ggtcatgtgg ggcactgaac tactacgaac tgatggtatt
     1201 gccattggtg agtttttgta ccttttaggt gtccaaccga tttggaatag caatggggat
     1261 gtgcagagta ctcccacctt aattccactg gaagacttga ccgtcaccat tgacggtgtt
     1321 accatgaaga gaccccgtat tgatgttttc accacagcag tcactggaaa tgagatctgg
     1381 atcaacctca tgaacaatgc tgttaaactg gctgcaaatg caccaggaga aactgataaa
     1441 gaaaataaca ttaaaaaaca tcttcttgaa aatgattctc tagatcgtgt atttggtctc
     1501 agagggctgg ttttagaggg aactggtgtc tgtgatttgg tacctaatac cagtaaatgg
     1561 agtaccagtg atgatctagc cagtgtatat ctttcccggg tttcttacgc ttggagatca
     1621 actgataacg gtatccaaat tagtcagaat aggaacactt tccagtattt actaagaaac
     1681 atggacatgg taactcagac cattgatagt acctggagat tactggacac tgatgactac
     1741 tatgactggt ttggtggaat gttccttgca tcaactagtt taggtggaaa tcctgat
//
LOCUS       DAOQVW010000346         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_86255_length_1796_cov_2.036760, whole genome shotgun sequence.
ACCESSION   DAOQVW010000346 DAOQVW010000000
VERSION     DAOQVW010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86255_length_1796_cov_2.036760"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /locus_tag="PKK85_07260"
     CDS             complement(<1..1742)
                     /locus_tag="PKK85_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNS25851.1"
                     /translation="MANSSKKKAKIKISGMNCASCALNVEKSLNNLKGVEEAQVNLNT
                     EEADVNYDPDKLNLRELENAVKKAGYGVVNEKITIKVGGMSCAMCVKAIEDVLGKLNG
                     ISQVNVNLASEKAYVTYNPKVTTIQDMREAIEDLGYQYLGIEGEETEDLEEKLRKEDL
                     KGKKNRIIVGFTVSIPLLFLMYSNVMLPIPMPYFMLIVTVIPFIYVSYPIFIAASRSL
                     RNGLLDMDVMYSLGIGVAFISSILGTFQVVLTPEFMFYETALMLAAFLMLGRYLEARA
                     KGNTSTAIKKLVGMQPKTATVIRNDQELEVKIEEVETGDLVLVKPGEKIPADGVVEEG
                     ESYVDESAITGEPVPVHKNPGENVVGGTINQNGVLKFRATRIGKDTVLAQIIKLVEVA
                     QGSKPPVQRIADRAVNYFIPTILTIALISFIVWYFILGSTLLFGLTVLISILVVACPC
                     ALGLATPTAVTVGIGRGAELGILVKNGEALEVSEKISTVLFDKTGTLTKGKPEVTDVV
                     ANDLLASETDQNTLLKLAASVEKNSEHPLAEAVVKKAESEGIGLIKTSEFTSFGGKGV
                     KATLDDEDVIIGNRL"
BASE COUNT          414 a          437 c          363 g          582 t
ORIGIN      
        1 agtctgtttc cgattattac atcctcatca tccaaggtgg ctttcacacc tttaccaccg
       61 aaactggtga attcactggt ctttataagt cctattccct ctgattctgc cttctttacc
      121 acggcttctg ccagggggtg ttcagagttt ttctctacac tggccgctag ttttaagagg
      181 gtattttggt ctgtttcact ggccaataga tcgttggcca ctacatcggt tacctctggt
      241 tttcccttgg taagggttcc tgttttatcg aagaggactg tgcttatttt ttctgatacc
      301 tccagtgctt caccgttttt taccagtatc cccagttctg caccccgacc aatgcccacc
      361 gtaactgcgg tgggggtggc caggcctaaa gcacagggac aggccactac caatatggag
      421 atgagtacgg ttagtccgaa aagcagggtg cttcccagta taaaatacca cactataaag
      481 gagattaatg cgattgtaag gatggtgggg atgaaatagt tcactgccct gtcagcgatt
      541 ctctgtaccg gtggtttgga tccctgagct acttccacca gttttatgat ctgggccagg
      601 accgtgtctt tacctatcct ggttgccctg aatttcagca caccattctg gttgatggtt
      661 ccgcccacaa cattttctcc cgggttttta tggacgggta ctggttctcc ggtgatggct
      721 gattcatcaa cgtagctttc accctcttcc actaccccat ctgccgggat tttctcacca
      781 ggctttacca ataccaggtc accagtttct acttcctcga tcttgacttc cagttcctgg
      841 tcattgcgaa ttacggtggc tgttttcggc tgcattccaa cgagtttttt tatcgcagtt
      901 gaggtgttac ccttggcccg ggcttctaag tatcgcccca acatgaggaa tgccgccagc
      961 attaatgcag tctcgtaaaa catgaattct ggtgtgagca cgacctggaa ggtgcccagg
     1021 atacttgaaa taaatgccac tccaattccc agggagtaca taacatccat atccaaaagg
     1081 ccatttctaa gcgatctgga tgctgcgatg aatatgggat agctcacgta gatgaagggg
     1141 attacagtta caataagcat gaagtagggc atggggatgg gtaacatcac gttagaatac
     1201 atcaggaata agaggggaat tgatacggta aaaccaacta taattctgtt cttttttccc
     1261 tttaaatcct cttttctaag tttttcttca aggtcttctg tttcttcccc ttctatcccc
     1321 agatactggt atcctaaatc ttctatggct tccctcatat cctgtatggt ggtaacttta
     1381 gggttataag tcacataggc cttttctgat gccaggttaa cgttcacctg gcttattcca
     1441 tttaatttac ccagaacatc ttctatggcc ttcacacaca tagcgcagct catgccgccc
     1501 actttgatag ttattttttc gtttacaaca ccataacctg ctttttttac ggcattttcc
     1561 agttcccgta aatttaattt atctgggtcg taatttacat ctgcttcttc tgtgttcagg
     1621 ttcacctgag cttcttctac acctttcagg ttgttgagtg atttttcaac gttaagcgca
     1681 caggaagcac agttcattcc tgaaatcttt atttttgcct tttttttgga tgaatttgcc
     1741 attagatagc ctctttaacc cttcagaaaa aaatttaccc tcgataaaaa ttcaca
//
LOCUS       DAOQVW010000347         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_86538_length_1792_cov_3.041451, whole genome shotgun sequence.
ACCESSION   DAOQVW010000347 DAOQVW010000000
VERSION     DAOQVW010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86538_length_1792_cov_3.041451"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKK85_07265"
     CDS             complement(<1..266)
                     /locus_tag="PKK85_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034716.1"
                     /note="allows the formation of correctly charged
                     Gln-tRNA(Gln) through the transamidation of misacylated
                     Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA
                     synthetase; forms a heterotetramer of GatD2E2; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase GatDE subunit E"
                     /protein_id="HNS25852.1"
                     /translation="MDYDELGLKMGLEIHQQLDTKTKLFCPCECELTDKKPEYRILRN
                     LRPTQSELGKIDRAAFEESRRKLTFLYDTYSHYTCLVESDDEPP"
     gene            complement(309..1634)
                     /gene="gatD"
                     /locus_tag="PKK85_07270"
     CDS             complement(309..1634)
                     /gene="gatD"
                     /locus_tag="PKK85_07270"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HNS25853.1"
                     /translation="MNYQGLARKFLESQDINIGDRILIKKRDLEYQGMLLDRAEDADD
                     QHIVIKLDNGYNIGINISETSLELLERGDAPKIEFAPLEFQKDPEKMDISIISTGGTV
                     ASIIDYKTGAVHPAFTAEDLLRATPELLDEANIKGKAIMNILSENMQPDYWVETARSI
                     ADEINQGAQGVVVAHGTDTMHYTSAALSFILETPVPVILTGAQRSSDRPSSDAFLNLM
                     NSVTAARSDLSEVMVCMHANENDNYAYLHRGTKVRKMHTSRRDTFQSVNSSPLARIQE
                     GRVKVMDQYYSYKKRGEYKLEVNDSLEPKIALIKIFPGVSGDLVDYHIDKGYQGLVLE
                     GTGLGHCPEDVMPSLHRAADEEIPVVMTSQCLYGRTNLNVYSSGRQLLKAGVIPSGDM
                     LPETAYVKLMWAMGQTRDRDGVKRIMESNLRGELSDKSSSQHFLKDYGE"
BASE COUNT          463 a          413 c          339 g          577 t
ORIGIN      
        1 tggggtggct catcatcaga ttccaccagg caagtgtagt gggaataagt atcatagagg
       61 aaagttagtt tacgacggga ttcttcaaat gcagcccggt caatcttccc cagttcactt
      121 tgggtgggtc ttaagtttct taatatccga tattcaggtt ttttatcagt taactcacat
      181 tcacatggac agaataactt ggtcttggtg tcgagttgtt gatgtatctc caggcccatt
      241 ttgaggccca attcatcgta atccatctta atcaccattt ataaagttaa ttaccaatac
      301 gggcttctct attctccgta atcttttaaa aaatgttgac tggaggattt atcacttaat
      361 tctcccctca aattgctttc catgattctc ttaaccccat ctcggtcccg ggtctgcccc
      421 atagcccaca tgagtttcac ataagctgtt tccgggagca tgtccccaga aggaataacc
      481 cctgccttaa ggagttggcg accgctactg taaacgttga gattagttct gccgtataga
      541 cactgggagg tcatcaccac cgggatttct tcatcagcag ccctatgcaa ggagggcatc
      601 acatcctcgg gacagtgccc tagacctgtt ccttctaaca ccagcccctg ataaccttta
      661 tcaatatggt aatcaacaag atccccagat acgccaggaa atatctttat taatgcaatt
      721 tttggttcta gcgaatcatt aacttctaat ttgtattctc cacgtttttt ataggagtaa
      781 tattggtcca tgactttgac cctgccctct tgaattcttg ccaaggggga gctgttgaca
      841 ctctggaagg tatctctacg gctggtgtgc atcttcctca ccttggttcc ccggtgcaga
      901 taggcgtagt tatcattctc attggcatgc atacagacca tcacctcaga taggtctgat
      961 cgagctgcag tcaccgaatt catgaggttc aaaaaggcat cagaggatgg tcggtcagaa
     1021 ctccgctgag caccggttaa aataactggc actggtgttt ccaggataaa actcagggcc
     1081 gctgacgtgt aatgcatggt atcggttcca tgagctacca ccactccctg ggctccttgg
     1141 ttaatttcat cagcaatgga acgggcggtt tctacccaat aatccggctg catgttttcg
     1201 cttagaatgt tcattatggc cttacccttt atgttggcct catccagaag ctccggtgtg
     1261 gctcttaaaa gatcttcagc agtgaatgca gggtgaacag ccccagtttt gtagtcaata
     1321 atggaggcca cggttcctcc agtagaaatg attgatatat ccattttttc tggatctttc
     1381 tgaaattcca gtggggcaaa ctcaatttta ggagcatcac ccctttcaag gagctctaaa
     1441 ctagtttcag atatattaat accaatatta tatccattat ccagttttat tacgatatgt
     1501 tgatcatcag catcttcagc ccggtccaac aacattccct ggtattctaa atctcttttt
     1561 ttgattaaaa tgcgatcccc tatatttata tcctgtgatt ctaaaaattt tctggctaat
     1621 ccttgataat tcatagcatc cctcgtgaag tcacatgaaa taatttatcc agatattgct
     1681 tgtttttatg tgttaaaaaa tttttaaact ttgattccat aatttttttc aactacacta
     1741 cagtttttat ccattctaaa aaaaatgtat tcacctttct catttttttc ta
//
LOCUS       DAOQVW010000348         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_86562_length_1792_cov_2.371906, whole genome shotgun sequence.
ACCESSION   DAOQVW010000348 DAOQVW010000000
VERSION     DAOQVW010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86562_length_1792_cov_2.371906"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1245)
                     /locus_tag="PKK85_07275"
     CDS             complement(64..1245)
                     /locus_tag="PKK85_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25854.1"
                     /translation="MRIFNNHPTILVLSGGVELPGIGELEKILEDLGKADPESFQIFS
                     DIFLVESSQGNMLFPESFKEKAREYFGIKKDGRVIESQDQVLGRLERQGIISIYNHWT
                     GEGSLFNSLRAERPGISRDEWENELRVIDEQVKSSTPGCDFCQPEKYTPEDVFGRIKG
                     RHCITGANIAKYDAWSSIIFFNRHHPLRFNYKEVADYLETGWSWFLRVQEQDPDYGYP
                     TFLWNCLPRAGASQIHGHAQILMSKRPYAKFRALLDGVTRYRQEKDRDYFQDLYRCHQ
                     AVGLATGDVDLGVIASLTPVKEKELIILSQKSPYQSDRVKKTIYSVLRIFLDVLGVTS
                     FNLSIMGPGLDQEKDAPHLVRMVDRGSMLKNTNDIGAMELYGSSVIANDPYQVIKALR
                     Q"
     gene            complement(1363..>1792)
                     /locus_tag="PKK85_07280"
     CDS             complement(1363..>1792)
                     /locus_tag="PKK85_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HNS25855.1"
                     /translation="AHLEEKALKAIYDFQNSMRIMGKDKNVLIKGLGLSFLIWFLEIL
                     RVYIIFAAFNVDVSLLVIAEVFIISTLIGFIPLLPGGLGAVDGLMIILFSAAGIPPST
                     SAAATIIERLISFWMTTILGLISLPYFGAAAVEKVFKKGH"
BASE COUNT          420 a          461 c          417 g          494 t
ORIGIN      
        1 tatcatcttt aaacttttca agctcatcca ggtgttggaa taggtatctg gacatggaat
       61 tcctcactgc ctcagagcct taatcacttg gtaggggtcg ttggctatga cgctgctgcc
      121 gtaaagctcc atagccccta tatcgtttgt gttcttcaac atactccccc gatcaaccat
      181 cctcactagg tggggtgcat ccttctcctg gtcaagaccc ggccccatga tggatagatt
      241 gaaactggtc acacccagca catctaaaaa tattctaaga acagagtaaa tggttttctt
      301 aaccctatca gattggtagg gcgatttttg gctcaggatg atgagctctt tttctttaac
      361 cggagtcagg ctggctataa cccccaagtc cacatctcct gtggccaggc ccactgcctg
      421 gtggcacctg taaagatcct ggaagtaatc cctatccttc tcctgcctgt accgtgttac
      481 accatccaac aatgccctga acttagcata aggcctttta ctcatgagta tctgggcgtg
      541 gccatgtatc tgtgatgccc cagcccgggg gaggcagttc cataaaaagg tgggatatcc
      601 ataatccggg tcctgttcct ggacccttaa aaaccaggac caccctgttt ccaggtaatc
      661 agccacctcc ttgtagttga acctgagggg gtggtggcgg ttgaaaaata tgatactact
      721 ccaggcatca tatttggcaa tgttggctcc agttatacag tgtctgccct ttatcctgcc
      781 gaaaacatct tcaggtgtgt atttttcggg ctggcaaaag tcacagccag gggtgctgct
      841 ttttacttgt tcatcaatga ctcggagctc attctcccat tcatccctgc ttatacctgg
      901 cctttctgcc cggagagaat tgaacaggga tccttccccg gtccagtgat tgtagatgct
      961 gattatgccc tgcctttcca ggcgccccaa tacctggtcc tgggattcaa tgactcggcc
     1021 atctttttta attccaaagt attctctcgc cttttccttg aagctttccg gaaacaacat
     1081 attaccctgg ctggattcaa ccagaaaaat gtcactgaaa atctggaatg actcagggtc
     1141 agcttttcct aggtcctcca ggattttttc caattcccct atacccggca attctacacc
     1201 tccacttaat actaatatgg tagggtgatt attgaatatt ctcatgggga acttcaccat
     1261 gtaccatccc ttaccagacc ctggtgcagt ttatggtggc tggggaggaa cagggcagcg
     1321 aacccccgga tcttggcagg tcctgtgggg gggctgtcat ggtcaatgtc cctttttgaa
     1381 caccttctct actgctgcgg ccccgaagta gggtaaagat atcaggccca ggatggtggt
     1441 catccagaag gatattaatc tctctatgat ggtggctgct gcacttgtgg atgggggtat
     1501 gcctgctgct gagaagagta taatcatcag tccatccacc gcccccagac cacccgggag
     1561 taggggtatg aatccgatga gggtggatat gatgaagacc tcagctatga ccagtaggga
     1621 tacgtccacg ttgaaggccg caaatataat gtaaacccgg aggatctcca aaaaccagat
     1681 gagaaaggaa aggcccaggc ctttgatcag cacgtttttg tcttttccca tgatacgcat
     1741 gctgttctgg aagtcataaa ttgccttcag ggccttttct tccagatggg cg
//
LOCUS       DAOQVW010000349         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_86627_length_1791_cov_2.669931, whole genome shotgun sequence.
ACCESSION   DAOQVW010000349 DAOQVW010000000
VERSION     DAOQVW010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86627_length_1791_cov_2.669931"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKK85_07285"
     CDS             complement(<1..346)
                     /locus_tag="PKK85_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS25856.1"
                     /translation="MRSEELFNEAKKYLPGGVNSPVRSYQPYPFFAQEAEGPRIRDAD
                     GNTYLDYCLAYGPMVMGHANPVVMGAVLNQMSRGTAFGVPTEKETELAREVVNRVPCA
                     EMVRFVSTGTEAT"
     gene            complement(352..975)
                     /locus_tag="PKK85_07290"
     CDS             complement(352..975)
                     /locus_tag="PKK85_07290"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HNS25857.1"
                     /translation="MFMGASTKEGQEIAEKSREIVRSLISKKIKHLKPGEQVIVERIV
                     HSTADPEYAELTKISEDFLEESLKALKGIEDILTDINMVKVGITSYPGEVKCYIDHPL
                     THKIARKREITRAAAAVEVALRDGFHGIVVIGNSPTSLLKVIELQKQYPDRIKSVIGV
                     PVGFVGAAESKKSLQGTPIPYLVTIGPKGGTPIAVAAANALINLSKE"
     gene            1196..1459
                     /locus_tag="PKK85_07295"
     CDS             1196..1459
                     /locus_tag="PKK85_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HNS25858.1"
                     /translation="MKEQASEVGGQVKQTIATLMTTAFGLIAALAWNEAIKAIINEFF
                     AKGEGVTGLLIYAILITIIAVVASLIIGWALGKPPVQEVKIVG"
BASE COUNT          494 a          359 c          352 g          586 t
ORIGIN      
        1 tagtagcctc ggttccggtg ctcacgaaac ggaccatttc ggcacagggc accctattca
       61 ccacttctcg ggccagttct gtttccttct cagtgggtac tccaaaggca gttcctctgg
      121 acatttggtt taaaactgcc cccatcacca caggattggc atggcccatt accattggcc
      181 cataggccag acagtaatcc agataagtgt taccatcggc atcgcgtatg cgtggcccct
      241 cagcttcctg ggcaaagaaa ggataaggtt ggtaggacct gactggggag ttaactccac
      301 cgggcagata cttttttgct tcattaaaga gttcttctga tcgcaattta ttcactcctt
      361 ggataaatta attaacgcat tggcagcggc aacagcgata ggagtaccgc cttttggccc
      421 tatggttacc aaatagggga ttggtgtccc ttgaagagat ttttttgact ctgcagctcc
      481 gacaaatcct actggaacac cgatcactga ttttatccta tcggggtact gcttctggag
      541 ctcgattacc ttcaaaagtg aagtagggga gttccctata accacaattc catgaaaccc
      601 atctctgagg gccacttcca cggcagccgc agcacgggtg atttcccttt tcctggcgat
      661 cttatgggtt agaggatggt caatgtaaca cttaacttcc cccgggtaag aggtgatacc
      721 taccttaacc atgtttatat cggttaatat atcttcaatt ccttttaagg cctttaagct
      781 ctcttccaga aaatcttcac ttatttttgt taattctgca tattctggat cagcagtgga
      841 atgtacaata cgttccacaa tgacctgttc tccaggtttt aagtgtttaa tcttcttaga
      901 gataagtgat ctaactattt ctcgactttt ttcggctatt tcttgccctt ctttggtgga
      961 tgcgcccatg aacatgattt cacataccct gataaaatat aattgaaatt tttttgactg
     1021 tatataatat cttctttagt tactctggaa gtgctttttt aaatttatat tctttcatta
     1081 tatttccatt agatcaaaat aactttcaga ttttaacccc tttaaggcaa aaatattatt
     1141 acagtctatt aacataacga tgtgtgttgt cctttgttta aagagggtgt gaaaaatgaa
     1201 agaacaagca agtgaagtag gtggccaagt aaaacaaact atcgccacac taatgacgac
     1261 tgccttcggg ctaatcgcag cattagcttg gaacgaagct attaaagcca taataaacga
     1321 attttttgcg aaaggagaag gtgtgactgg tctactgatt tacgccattt taattaccat
     1381 aatcgctgtt gtggcatctt taatcattgg gtgggcccta ggtaaacctc cagtacaaga
     1441 agtaaagatt gtggggtaga gggattctat tccattatca catttttttt gattgaatta
     1501 actttgagct tgtctcaaga ttaacttaac ttttttttgg atatcttatt tttttaaggg
     1561 ttatattcat atttagggag ttatcatatt ttatttagtt gtttatagtt ttttatggct
     1621 ttgaaggaag atcgtattga ttctttaaag taccgtcgat aagcaatagc cccatttcaa
     1681 catctgtgaa gttatttaca tgcctcagat caggaaaagt gtgttggtaa aaatcaaggt
     1741 aaaaataaca acagactacg gagatgtttt accaaaaaaa tatggcactt a
//
LOCUS       DAOQVW010000350         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_86757_length_1789_cov_4.181084, whole genome shotgun sequence.
ACCESSION   DAOQVW010000350 DAOQVW010000000
VERSION     DAOQVW010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86757_length_1789_cov_4.181084"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKK85_07300"
     CDS             complement(<1..476)
                     /locus_tag="PKK85_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HNS25859.1"
                     /translation="MVKIAQISCGTDYSGVQKEIEKAAATFGAEMIIPEADLDYINEA
                     YQKFGFNAASSSIRLMIARAMSLVEGKSDADAVFISTCFRCAEGALVRNEIRRFIQQY
                     TNLPVVTYSFTERTKADELFIRMEALSTIVARKSILAREKQEGLTLGIDSGSTTTKV"
     gene            complement(478..936)
                     /locus_tag="PKK85_07305"
     CDS             complement(478..936)
                     /locus_tag="PKK85_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HNS25860.1"
                     /translation="MKIAIFPPNSLILADLVERKGHEPLVLQKEIRKKVTDPEIDSPP
                     FNITEDEPIKGLKYAAIEVPSGVRGRMAIFGPLIEEAEAAIIMEDAPFGFGCIGCART
                     NELCMFVLRKQGIPVLELHYPETREETMEVVNQINTFLDNLESEDKEGGD"
     gene            complement(947..1309)
                     /locus_tag="PKK85_07310"
     CDS             complement(947..1309)
                     /locus_tag="PKK85_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HNS25861.1"
                     /translation="MNLNSSSTGEDITELALAIHELVNRLPLTMRTKTQLGVRIEDGK
                     VVDYNYTGPTLELVLEKGEISRQVPKTGPYQGTPVVVVPIHEAGEVIAALGVVDITQG
                     IFSDIMEITKRPEDVKKK"
     gene            complement(1306..1725)
                     /locus_tag="PKK85_07315"
     CDS             complement(1306..1725)
                     /locus_tag="PKK85_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HNS25862.1"
                     /translation="MSEEDEVTRMVILGPAAQVSQSQVVGELHMLELPLTIKSTCYGA
                     VVHGKEEDVMKAIQEIRKLDPANIFTKDRGFPPGDPRRCRAKRGGAREGFHQLEKEFE
                     LLGYVGEALEWPEPVILKKHSKISPEEFKKIAEECEP"
BASE COUNT          351 a          485 c          420 g          533 t
ORIGIN      
        1 acttttgtag tggtggagcc ggagtctatg cccagggtga ggccttcctg tttctcccgg
       61 gccagtatac tcttccgggc cacgatggtg gataaggcct ccatacggat gaagagttca
      121 tcggccttgg tcctttctgt gaaggagtag gttaccaccg gtaggttggt gtactgttgg
      181 atgaaacggc gaatctcatt tctaaccagg gcaccctcgg cgcagcggaa gcaggtggaa
      241 atgaacactg catcggcatc ggacttaccc tccaccaggg acatggcccg ggcgatcatt
      301 aaccgtatgc tgctgctggc ggcgttgaac ccaaacttct ggtaggcctc gttaatgtaa
      361 tccaggtctg cttcgggtat gatcatctcc gcaccaaagg tggcggcagc tttttcaatc
      421 tccttctgca ctccactgta atcagtaccg caagaaattt gtgctatctt caccatctca
      481 gtctcctccc tctttgtctt cggattccag gttatccagg aaggtgttga tctggttcac
      541 cacttccatg gtctcctccc tggtttcggg gtagtgcagt tccagcaccg gaattccctg
      601 tttacggagg acgaacatgc acagttcatt ggtccgggca cagccaatac agccaaatcc
      661 gaatggagcg tcttccatga tgatggctgc ttcggcttcc tcgatcagcg gcccgaatat
      721 ggccatacgg ccccttacac cagagggaac ctctattgcg gcatatttaa gccccttaat
      781 tggttcatcc tcggtgatgt tgaagggagg tgaatctatc tctggatctg tgactttttt
      841 ccttatctct ttctggagaa ccagtggctc gtggcctttc cgctccacca aatctgccag
      901 tatgagtgag tttggtggaa aaattgctat tttcatgtta ttctccttat ttctttttta
      961 catcttccgg tcgcttggta atctccatta tgtcactgaa aatgccctgg gtgatatcca
     1021 ccactcccag tgcagctatc acttcccctg cttcatggat gggaaccacc accacagggg
     1081 tgccctggta tggtccggtt ttaggtacct gacgggatat ttctcccttt tcaaggacca
     1141 gttccagtgt gggaccggtg taattgtaat caacaacttt tccatcttct atccgaacac
     1201 ccagttgtgt tttggtccgc atggtcaggg gcaggcggtt aaccagttcg tgtatggcca
     1261 gggccagttc cgttatatct tctccagttg atgaggaatt aaggttcatg gttcacactc
     1321 ctctgctatt tttttgaact cctcaggact tatcttacta tgttttttta gtatcactgg
     1381 ttccggccac tccagggcct ccccaacgta tcccaacagt tcgaattctt tctccagctg
     1441 gtggaatccc tccctagccc ctcctctctt ggctcggcag cgcctggggt ctccaggtgg
     1501 gaatccccgg tctttagtga atatgttggc tgggtccagc tttcggatct cctggatggc
     1561 tttcataaca tcctcttctt tcccatggac cactgccccg taacaggtgg attttatggt
     1621 taggggaagt tccagcatgt gcagttctcc caccacctgg ctctggctga cctgggcagc
     1681 tggtcccaga ataaccatcc gtgtaacttc atcttcttcg gacataaact gtttccccct
     1741 ctttaaattt tttaagatta tccatgccct taatcacttt tcctaggat
//
LOCUS       DAOQVW010000351         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_86963_length_1787_cov_3.525982, whole genome shotgun sequence.
ACCESSION   DAOQVW010000351 DAOQVW010000000
VERSION     DAOQVW010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86963_length_1787_cov_3.525982"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKK85_07320"
     CDS             <1..287
                     /locus_tag="PKK85_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNS25863.1"
                     /translation="LTDDGLASGYTLLAALQALENQKVKERIVATPTAHLDALEMLKS
                     HLDTVFCPNVRSGWSFAVADAYHYWSDVEEDEAVALLKKFRQEKERWNEK"
     gene            277..411
                     /locus_tag="PKK85_07325"
     CDS             277..411
                     /locus_tag="PKK85_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25864.1"
                     /translation="MKSRLSKKMVGAQHHPQVKIIKNLIFPVHRVHGPGHLGAFLEKY
                     "
     gene            485..739
                     /locus_tag="PKK85_07330"
     CDS             485..739
                     /locus_tag="PKK85_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25865.1"
                     /translation="MPGTTDPLTSLNFIFCIIILIMGYWGFRKYEEPLLFYIGMAFGL
                     FGVSHLALISGYPSNLWVLILIRTVAYLLVIFALFQAIRR"
     gene            881..>1787
                     /locus_tag="PKK85_07335"
     CDS             881..>1787
                     /locus_tag="PKK85_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNS25866.1"
                     /translation="MELLLLKDVVIILGLSVAVLLLFSRIKIPSILAFFVSGVLAGPH
                     GLGLISSVSQVELIAELGVIFLLFTIGLDFSMDKFSQIKRYALVGGSLQVGLTTLTVT
                     MISLALGFPISESIFMGLLVSFSSTAIVLRILQDKKQLDSVHGRTALGILIFQDLAVV
                     VVILLTPLLAGSPSNSLGDWPLLIGLGVGLVAFTIISSRWLVPWLLHYIAHFKRRDLF
                     LLTIIVICFGITWITTALGLSPALGAFLAGLIISNTEYSYQALGNVLPFQDIFMSIFF
                     ISIGMLLNVGFLLENLFLILLLTLAV"
BASE COUNT          471 a          400 c          402 g          514 t
ORIGIN      
        1 tacttaccga tgatggccta gcatcgggtt acactttact agccgccctc caggcacttg
       61 aaaaccaaaa agttaaagaa aggattgtag ccacccccac cgcccaccta gatgcattgg
      121 agatgttaaa atcccatctt gatactgttt tctgccctaa tgttcgtagt gggtggagtt
      181 ttgccgtggc agatgcctac cattactgga gtgatgtgga ggaggatgag gcagtggcgt
      241 tactcaaaaa attccgccag gagaaagaaa ggtggaatga aaagtagatt atctaaaaaa
      301 atggtagggg cacagcacca cccccaggta aagatcatca aaaacctcat atttccagtg
      361 cacagggtac atggaccagg gcatttaggt gcatttctag aaaaatatta gactggctaa
      421 aaaaaatgcc accaaaggga taataaattg aaaggtgata aaatactagt gaggtgaaaa
      481 aaatatgcca ggcactactg atcccctgac atccttgaac ttcatatttt gtataataat
      541 tttgatcatg ggatactggg gttttcgtaa atatgaggaa cccctcctat tctatattgg
      601 tatggccttc ggacttttcg gagtgtctca cctggcactg attagcggat accccagtaa
      661 tttatgggtg ctaatactga tcaggactgt ggcatattta ctggttatat ttgccctgtt
      721 ccaagccata aggcgatgat ggatattttt taaattaact atcaattcca aaaaggattt
      781 gttattacac aaaatgctat cccatcaggg gactaagatc cttgttcaca gacggtggaa
      841 ccaatcctca ttttatatac attcacggga agtttaacac atggagttac tattattaaa
      901 agacgtggtc atcatattgg ggctgtcagt tgcggtgctt ctacttttta gccggataaa
      961 aataccatcc atcctggcct tctttgtaag tggagtgctg gccggccccc atggactggg
     1021 acttatcagt tccgtcagcc aagtagaact tatagctgaa ctgggtgtta tattcctact
     1081 tttcaccatt ggattggatt tttcaatgga taaattctcc cagataaaaa gatacgccct
     1141 agtggggggc tccctgcagg tggggttaac aacactgacg gtgactatga taagcctggc
     1201 attgggcttc cccatatcag aatctatttt catgggacta ttggtttcct tcagcagtac
     1261 cgccattgtc ctaaggatcc ttcaggataa aaagcagctg gacagtgtcc atggacgaac
     1321 cgccctgggg attcttatct tccaggacct ggctgtggtg gttgtaatcc tcttaacccc
     1381 cctcttggct ggctccccat ccaatagcct gggtgattgg ccgctgctta taggcctggg
     1441 tgtgggactg gtggccttca caatcatcag cagccgctgg ctggtgccct ggctgttaca
     1501 ttacatagcc catttcaaga ggagagacct gttcctttta accatcatcg tgatctgctt
     1561 tggaattacc tggatcacca ccgccctggg attatccccg gcattaggtg catttttggc
     1621 tggccttata atatccaaca ctgagtacag ttaccaggcc ctgggtaatg tgctgccctt
     1681 tcaggatata tttatgagca tatttttcat ctccattggt atgctcttaa acgtgggctt
     1741 cctcctggaa aatctattcc tgatactcct cttaaccctg gcagtta
//
LOCUS       DAOQVW010000352         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_87725_length_1778_cov_4.928613, whole genome shotgun sequence.
ACCESSION   DAOQVW010000352 DAOQVW010000000
VERSION     DAOQVW010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87725_length_1778_cov_4.928613"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..>1778
                     /locus_tag="PKK85_07340"
     CDS             422..>1778
                     /locus_tag="PKK85_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha"
                     /protein_id="HNS25867.1"
                     /translation="MKDDFWKSKDIKISIGEIEEKDTKKMGEEVPMGPTPKPHVTDLR
                     SWDMKLLDRYEPFYAPFCDMCCLCTFGKCDLTEKKGACGIDSAAQQARIVLLACNIGT
                     AAHAGHARHLVDHLINDFGADYPIDLGMNIDIEAPLIRTLMGKKPEKLGDLREVISYV
                     EEQMSHLLSACHTGQEGSSLDFESKALHSGMMDDLAREVGDIAQIVALNMPKGDENAP
                     LVELGVGTIDAKKPVVLCIGHNVLPGAGIMDYLDETGQEEDLEVCGICCAAIDVTRYN
                     DRAKVVGPLSKQLKFIRSGIADVIVVDEQCIRTDVLEEALNKNCAVIATTDKMCLGLP
                     DMTREDTDKIVSKLVNKEIPGALILDSEKVGEVSVKVAKILAPEREKLKFLPELDEIQ
                     KMASDCTECGWCNRVCPNSKPMMEAVVAASKGDFSKFQELYEYDVCYSCGRCEQECER
                     DL"
BASE COUNT          568 a          324 c          391 g          495 t
ORIGIN      
        1 gtagatcgcg cactgccata acggaataaa accatctggt atatgcatat tttttccctc
       61 ctaatttttt tgaggttaac gttaatcatc agattaatca ctgtcattgt tttttactag
      121 atcttcgggt attacctttt ttccaatcca tcaataaaag taacacttct tgttcctctg
      181 aatcatctaa atcagtgggg ttaaccttat tatatggtga ttaatttcat catatatacc
      241 ttcctataag tattactatc tggggatttt cagtattttt tgtaataaaa aggtaatact
      301 gaaaacaaat ctgaggctaa aaagtaatac acaaacttta tatatagaga tcaccacatt
      361 ccaaatatca ttataaatca ttaataattt ggtgatacac ttggcaccta aaccaaaaga
      421 tatgaaggat gatttctgga aatcaaagga tatcaaaatc tctatcggtg agatcgagga
      481 aaaagatacc aagaaaatgg gggaagaagt ccctatgggt ccaactccca agcctcatgt
      541 aaccgatctt cgatcatggg acatgaagtt gctggataga tacgaaccat tctacgcacc
      601 attctgtgac atgtgctgct tatgcacctt cggaaaatgc gacctaaccg aaaagaaagg
      661 agcatgtgga attgattctg ctgcacaaca agcccggatt gtacttctgg catgtaacat
      721 tggtactgcc gcccatgctg gacatgcccg gcacctggtg gaccatctga taaatgattt
      781 tggagcagat tatcccatag atctgggaat gaatatcgac atcgaagcac cattaatcag
      841 aactttaatg ggtaaaaaac ccgaaaagct gggtgatctt agagaagtaa tatcatatgt
      901 tgaagaacag atgtcacacc tattatctgc ttgtcatact ggacaagaag ggagcagtct
      961 agattttgaa tctaaagcac ttcattcggg gatgatggat gatttagcac gtgaagttgg
     1021 ggatatagct caaattgtgg ccttgaacat gccaaaaggg gatgaaaacg cccctctggt
     1081 tgaactgggt gtgggaacca tagatgccaa aaaaccagta gtgctctgca ttggtcataa
     1141 tgttctcccc ggtgcaggaa tcatggatta cttggatgaa acaggtcagg aagaagattt
     1201 agaggtctgt ggtatatgct gtgcagccat agatgttact cgttataacg accgggctaa
     1261 agttgtagga cccctatcta aacagctaaa attcattcgt agcggtatag cagatgttat
     1321 tgtagttgat gaacaatgta tacgcacgga tgttcttgaa gaagcattaa ataagaattg
     1381 tgccgtcatc gccaccaccg ataagatgtg tttaggactt ccggatatga cgagagaaga
     1441 taccgataaa atcgtttcaa aactagtcaa caaggaaata cctggggctt taatcctgga
     1501 ctcagaaaaa gtgggggaag tttccgttaa agtggctaaa atattagctc ctgaacgtga
     1561 aaaactaaaa tttttaccgg aattggatga aatacaaaaa atggcttctg actgtactga
     1621 atgtggatgg tgtaatcgag tttgtcccaa cagcaagccc atgatggagg ctgttgtggc
     1681 ggcaagtaaa ggtgactttt ccaagttcca ggagctatac gaatacgatg tatgttacag
     1741 ttgcggcagg tgcgaacagg aatgtgaaag ggaccttc
//
LOCUS       DAOQVW010000353         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_87858_length_1777_cov_2.558653, whole genome shotgun sequence.
ACCESSION   DAOQVW010000353 DAOQVW010000000
VERSION     DAOQVW010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87858_length_1777_cov_2.558653"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..560
                     /locus_tag="PKK85_07345"
     CDS             177..560
                     /locus_tag="PKK85_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNS25868.1"
                     /translation="MAEKCAVYRCQVCDHVIEVLHAGEGELVCCQVPMTLLVERNDDT
                     GQEKHVPVLEKTDSGFLVKVGEVAHPMEEEHCIDWIEVQADGRIYRQPLEAGDQPQAH
                     FDLDLDQVEQITLRSFCNIHGLWRA"
     gene            702..1193
                     /locus_tag="PKK85_07350"
     CDS             702..1193
                     /locus_tag="PKK85_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25869.1"
                     /translation="MRKPTLILALLILVAFSSGCIHSEVSRMDQLASNINDHLKGGDS
                     YYNQAVASTNRFQYDQALTQTNNALNEFDLGRSTTQEALIYARNSGKQVYIDYFQLTL
                     QELDLRLNATSELKMAIPYLQGNETTSANKHLDLANDYMKSSVDLATQKSQLVQDNMA
                     LFK"
     gene            1220..>1777
                     /locus_tag="PKK85_07355"
     CDS             1220..>1777
                     /locus_tag="PKK85_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNS25870.1"
                     /translation="MKKTCLECKGKGHLITSYQICESCHGSGVSSQVDLQDHVKGLPR
                     GARERYQLDVDEEVPCPECKGKGELEIKKTCPTCHGQGELNHCQKCGKQIDKGDYCED
                     CRSKQIVYQLHPSSDLGDLEVGAVYKGRVTKVVDFGVFVTFTKKVYGLMRMRASSYQV
                     GDEVFARVTEIKTHRGEVDLEPARIN"
BASE COUNT          488 a          432 c          492 g          365 t
ORIGIN      
        1 tgctggagca ggtggaaaag gacaccttcc agaagaagga ggaagaagtg gagtgggcct
       61 gtcccaagtg cggatacact gctaagggta aagaagcacc cgagaaatgc ccggcctgtg
      121 accatccaca ggaatacttc tacatacgct gcgaagagta ctagggggac gttaacgtgg
      181 cagaaaaatg tgccgtgtac cgctgccagg tttgtgacca tgtcatagag gttctccatg
      241 caggtgaagg agaactggtc tgctgccagg tccccatgac cctgctggtg gagagaaacg
      301 atgacaccgg ccaggaaaag catgtgccag tcctggaaaa aactgacagt ggcttcctgg
      361 ttaaggtggg ggaagtggcc catcccatgg aggaggagca ctgcatagac tggatcgaag
      421 tacaagccga tggaaggatc taccggcaac ccctggaagc cggggaccag ccccaggccc
      481 acttcgattt ggacctggat caggttgagc agatcaccct gcgcagcttc tgcaacatcc
      541 atggcctgtg gagagcctag ataatctcca tccatacaaa tttttttttc ttcttacttt
      601 tcttttgaca aaaaaaaaga tttaaacatt tattaaccct taagatgaaa catcatgcat
      661 aaatcctagt aaatctattg agctctgcgt ggatggtgaa catgcgtaaa ccgaccctga
      721 ttctggcact cctcattctg gttgcatttt ccagtggctg tatacactca gaagtatccc
      781 ggatggacca attggcctcc aacatcaacg atcacttgaa ggggggtgac tcctactaca
      841 accaggccgt ggccagcacc aacaggttcc aatatgacca ggccctaacc cagaccaaca
      901 atgccttaaa cgagtttgat ctgggccgca gcaccaccca ggaagctttg atctacgccc
      961 ggaatagtgg gaagcaggtt tacatagact acttccagct caccctgcag gaactggacc
     1021 tgcgattaaa tgccaccagt gagttgaaga tggccatacc ctacctgcag gggaatgaaa
     1081 ccaccagtgc caacaagcac ctggacctgg ccaatgacta catgaagagc tccgtggacc
     1141 tggccaccca gaagagccag ctggtgcagg ataacatggc cctgttcaaa tagttactaa
     1201 tttttttgag gatcatcata tgaagaagac gtgtttagag tgtaaaggta aaggacacct
     1261 gattaccagt taccagatct gcgagtcatg ccatggaagc ggtgtaagca gccaggtaga
     1321 cctgcaggac catgttaagg gactgcccag gggcgcccgg gagaggtacc agttggacgt
     1381 ggatgaagag gtgccctgcc cagagtgtaa gggcaaagga gaattagaga taaaaaagac
     1441 ctgtcccacc tgccatggac agggtgagct taaccactgc cagaagtgtg ggaaacagat
     1501 cgataagggg gattactgtg aagactgccg gagtaaacag atagtctacc agctgcatcc
     1561 ctccagtgat ctgggcgact tggaggtggg ggcagtctac aagggcagag tcaccaaggt
     1621 ggtggacttt ggagtgttcg tgaccttcac caagaaggtc tatggactga tgcgcatgcg
     1681 ggcctcctcc tatcaggtag gggatgaggt cttcgcccgg gtgacggaga tcaaaaccca
     1741 ccggggtgaa gtggacctgg aaccggcccg tattaac
//
LOCUS       DAOQVW010000354         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_89460_length_1757_cov_2.559342, whole genome shotgun sequence.
ACCESSION   DAOQVW010000354 DAOQVW010000000
VERSION     DAOQVW010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89460_length_1757_cov_2.559342"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..552)
                     /locus_tag="PKK85_07360"
     CDS             complement(208..552)
                     /locus_tag="PKK85_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25871.1"
                     /translation="MAEKRVEAIKEEMKSDLKKDYDEKVKAHLNCELEDGLEKELFDH
                     IIGQCSLTLDKELEEFIIARDEEELNDEVKNQIYGQAKEVLDKELEEFIIGKDEDEIQ
                     ERIQEQIRGKKS"
     gene            688..1413
                     /locus_tag="PKK85_07365"
     CDS             688..1413
                     /locus_tag="PKK85_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HNS25872.1"
                     /translation="MKGKILGDILLVREDQDDLQEYLKIPGVNKIVKLGHINGPHRKP
                     EVKLLWGEGTETIHKENRCLYKMDVSQVMWAKGNTAERIRLTKLVGEDETVVDLFAGI
                     GYFSIPIAVHARPTKVYAVEINHVSYHYLKENIKLNRVTDIVEPTLGNCRDVAPQNVA
                     DRVLMGYIGNTHEYIDVAINALRASGVIHYHEAVPEKLMFTRPVRRIQSAALDREVEI
                     INQRIIKKYAPGVYHVVIDAKIG"
     gene            1423..>1757
                     /locus_tag="PKK85_07370"
     CDS             1423..>1757
                     /locus_tag="PKK85_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNS25873.1"
                     /translation="MDLFEAIIGRRSIRRFKKDMVKDDIIMKVIEAGTWAPSAGNLQS
                     WEVVMVKNSETKEKLVTAAYLREFLAGAPIILVLCANQHECGASYNDRGVELYCIQDA
                     ACAGQNILLA"
BASE COUNT          551 a          311 c          355 g          540 t
ORIGIN      
        1 cgaaacgcaa atttttgtcg atcgattgtt gtaaattaag tctttaaaat gggaggacag
       61 ctcaaatggg agaaattaat ggagtaaaaa aattttttaa aaaccaaaaa aaagtattgt
      121 ttaaatatta cgcctattgg aatagggttc cccttacatt atgatactgt tacataaaag
      181 atatggtaag gagtgttttt attatgatta ggatttttta cccctgatct gttcctggat
      241 tctttcctgg atttcgtctt catcctttcc tattatgaac tcttccaatt ccttatccag
      301 gacttcttta gcttgtccat agatctgatt tttaacttcg tcatttagtt cttcttcgtc
      361 ccgtgctatg atgaactctt ccaattcttt atccagagtc agtgaacact gtccaataat
      421 atgatcgaac aactctttct caagaccatc ttctaattca caattaagat gagctttaac
      481 tttttcatca taatcttttt tcaaatcaga tttcatttcc tcttttatag cttctacacg
      541 tttttctgcc atagtaaata ccccttcctt tgataataga aatttattca cctattgtat
      601 aaatcttttt tctttttctc tgagcatttc tatccttaaa gaacaggaaa aattataatc
      661 aaaaatctcg ccatatatta taatgttatg aagggtaaga tcctcggcga catcctactt
      721 gtaagggaag atcaagatga tcttcaggaa tacctcaaga ttccaggagt aaacaaaatc
      781 gttaaattag ggcacatcaa tggtcctcac agaaagcctg aagtgaaact tttatgggga
      841 gagggcactg aaacaatcca caaagaaaat agatgtcttt ataaaatgga tgtatcccag
      901 gtgatgtggg ccaagggaaa cacggcggaa agaataagat tgaccaaact ggtaggggaa
      961 gatgaaactg tggtggactt atttgcaggt ataggctact tctccatccc cattgctgtt
     1021 catgctagac caactaaggt ttatgctgtg gaaataaacc atgtatccta ccattaccta
     1081 aaagagaaca tcaaacttaa cagggtaact gatattgttg aaccaacctt aggtaattgc
     1141 cgggatgttg caccgcaaaa cgtggcggat cgggttctaa tggggtacat aggcaatact
     1201 catgaataca tagatgtggc aattaacgca ctccgggcaa gtggtgttat ccactaccac
     1261 gaggcggtgc ctgaaaagtt aatgttcacc cggccagtgc gtaggataca atctgcggct
     1321 ctggatcggg aagtagaaat aatcaaccag cgaataataa aaaaatatgc tcctggagtt
     1381 tatcatgtgg taattgacgc taaaataggg taggtgttta caatggatct ttttgaagcg
     1441 ataatcggaa gaagaagtat tagaagattt aaaaaagaca tggtgaaaga tgatattata
     1501 atgaaggtga ttgaggctgg gacttgggct ccttctgctg gaaatttaca aagctgggaa
     1561 gtggtaatgg tcaaaaattc cgaaacaaag gaaaaactag taaccgccgc ttatttaagg
     1621 gagtttttgg ctggtgctcc gataatactg gtattgtgtg ccaatcaaca tgaatgtggt
     1681 gctagttata acgatcgtgg ggtggaactt tattgtatac aagatgcagc ttgtgctggt
     1741 caaaacatcc tccttgc
//
LOCUS       DAOQVW010000355         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_89621_length_1755_cov_3.260000, whole genome shotgun sequence.
ACCESSION   DAOQVW010000355 DAOQVW010000000
VERSION     DAOQVW010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89621_length_1755_cov_3.260000"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKK85_07375"
     CDS             <1..226
                     /locus_tag="PKK85_07375"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNS25874.1"
                     /translation="FGVQTMLTFNYKRAFSRIILVGSVINIILTLIMAPFYTAVGISV
                     AMVVTEIYITLAMYLYLRGKGINLLELKRV"
     gene            219..1388
                     /gene="glf"
                     /locus_tag="PKK85_07380"
     CDS             219..1388
                     /gene="glf"
                     /locus_tag="PKK85_07380"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HNS25875.1"
                     /translation="MYEHIIVGAGLAGCVLAERIANVLGEKVLVVEKRDHLGGNCYDY
                     YDEAGLLVHKYGPHIFHTQLEEVWEYLSRFTKWRPYQHRVLGCVDGKKIPIPFNLTSL
                     HQSFTPEVAQRMEDKLVENYGREVQVPILELKDCDDEDLKLLADYVYRKVFLNYNRKQ
                     WGLMPEELDPSVTARVPVLVSHDDRYFQDPYQGIPREGYTKIFEKMLSNPHIEVLLNT
                     DYKEVLKFKEGVIHCKGKNFKGKLIYTGEIDYLFGYQYGRLPYRSLQFVMETLDQEFY
                     QEVATVNYPNEHRYTRITEFKHLTGQKHGQTTITREYPQSYQAERGDVPYYPIPKEEY
                     QEIYERYRKKASQYSNLILVGRLAEYQYLNMDLVVDRALTIFQEKLAPGKKTGEK"
     gene            1393..>1755
                     /locus_tag="PKK85_07385"
     CDS             1393..>1755
                     /locus_tag="PKK85_07385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS25876.1"
                     /translation="MTPETVCAVVVTYNRRELLIECLDALRNQTRPVDAVYLVDNAST
                     DDTPELLLEKGYLDLVPETGEEAWERTLKRDDLVVHYLRLSHNQGGSGGFHQGVKRAY
                     QQGYQWLWLMDDDARPQED"
BASE COUNT          449 a          454 c          501 g          351 t
ORIGIN      
        1 gttcggggtg cagaccatgc tcaccttcaa ctacaaacgg gcattctcca ggataatcct
       61 ggtaggtagt gtgataaaca tcatattaac cctgatcatg gccccctttt acacggcggt
      121 gggtatatct gtggccatgg tggtaaccga aatctacatc accctggcca tgtacctata
      181 ccttaggggt aagggaataa acctcttgga gctaaaacgt gtatgagcac ataatagtag
      241 gagctggact cgccggctgt gtcctggccg agcggatagc caatgtactg ggagagaaag
      301 tcctggttgt ggagaaaagg gaccacctgg gtggtaactg ttacgactac tacgacgagg
      361 ccggattgct ggtgcacaag tacggccccc acatcttcca cacccaactc gaggaggtgt
      421 gggagtacct gtcccggttc acaaagtggc gtccctacca gcaccgggtc ctgggctgcg
      481 tggatgggaa gaagatcccc atccccttca acctaaccag cctgcaccag tccttcactc
      541 cggaggtggc ccagaggatg gaggataaac tggtggaaaa ctatggccgg gaggtacagg
      601 tccccatcct ggagttgaaa gattgtgatg atgaggatct taagttactg gcagactatg
      661 tgtaccggaa ggtgttttta aattacaata ggaagcagtg ggggttgatg cccgaggagc
      721 tggacccctc ggtcacggcc cgggtgccgg tcctggtatc ccatgatgac cgctacttcc
      781 aggaccccta ccaggggatc ccccgggagg gctataccaa gatcttcgag aagatgctca
      841 gtaatcccca catagaagtt ttgctgaaca ctgactataa ggaagtttta aagttcaagg
      901 agggagttat acactgtaag gggaagaact tcaagggtaa actgatctac accggtgaga
      961 tcgactatct cttcggctac cagtatggca ggttgcccta ccggtccctg cagtttgtaa
     1021 tggaaaccct ggaccaggaa ttctatcagg aggtggccac ggtcaactac cccaatgagc
     1081 accggtacac ccggatcacc gagttcaaac acctcacagg ccagaagcat ggacagacca
     1141 ccatcacccg ggagtacccc cagagttacc aggcggaaag gggtgatgtg ccctactacc
     1201 ccataccaaa ggaagagtac caggagatat atgagaggta ccggaaaaag gcaagccagt
     1261 actctaacct tattctggtg gggaggctgg ccgagtatca gtacctcaac atggacctgg
     1321 tggtggaccg ggccctgacc atcttccagg agaaactggc ccctggaaaa aaaacaggag
     1381 aaaaatagcc ccatgacccc tgaaacggtg tgcgccgtgg tggtgaccta caaccgccgg
     1441 gaactcttaa tagaatgtct ggatgccctt agaaaccaga ctcgaccagt tgatgcggtt
     1501 tacctggtgg acaatgcctc caccgacgac acccctgagc tgcttttgga aaagggctac
     1561 ctggacctgg tcccagaaac cggggaggaa gcctgggagc ggaccctgaa gagggatgat
     1621 ctggtggtgc actacctgcg cctctcccac aaccagggag gatccggcgg cttccatcag
     1681 ggggtgaaaa gggcctacca gcagggttat cagtggctgt ggctgatgga cgatgatgcc
     1741 cggccccagg aggat
//
LOCUS       DAOQVW010000356         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_89952_length_1751_cov_3.300118, whole genome shotgun sequence.
ACCESSION   DAOQVW010000356 DAOQVW010000000
VERSION     DAOQVW010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89952_length_1751_cov_3.300118"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1364
                     /locus_tag="PKK85_07390"
     CDS             162..1364
                     /locus_tag="PKK85_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HNS25877.1"
                     /translation="MFREIPVTYFGCTHRAVNPEETIIRVEKKLKYAGVTRIADITHL
                     DRVGIPVYSAIRPGAAEGAVSVYAGKGATKIQAKASAMMEAFERYSAEFQDSDKDNVV
                     SQLYEPGECVDPNSLIIPYRTSDPRSEKIEWITALDARTDKKSLIPANAVYHPYHPQR
                     GVHLFKSNTNGLASGNVLEEAVFHGLMEVVERDAWSIFEARKKHKKEVICDKTENKLI
                     NTILTKFREKGVDIKLVDLTADIKITTIAAVADDKVLKDPALLTMGVGTHLDPEIAVI
                     RALTEVAQSRATQIHGTREDTTRAIFMRKAGYERMKRINKHWFGDYKEKIKLSQLKNK
                     STTSFKDDLEISLDELKKVGIEQIFYVDITRPEIDIPVVRVIIPGLEVYAVDPERMGR
                     RIMDKEIS"
BASE COUNT          557 a          333 c          390 g          471 t
ORIGIN      
        1 gtcaacagtt ttttggcact tttcaacctc ctacctattg gtgtgtttga tggagccaag
       61 gtttttaaat ggaacccagt ggtatgggtc atttcagcag gattatcctt cttaatggtc
      121 gcttattcca tgatgggaag gctaatttag gatctggtta aatgtttaga gaaattccag
      181 tcacgtactt tggatgcaca catcgggctg ttaatccaga agaaaccatc attagggttg
      241 aaaagaagtt aaaatatgcg ggagtaacca ggatagccga tatcactcac ctagaccggg
      301 tgggaatacc ggtgtactct gccatcaggc ccggagcagc cgagggtgca gtgagtgtat
      361 acgcaggtaa aggagctacc aaaatacaag ccaaggcttc ggctatgatg gaagcttttg
      421 aaagatattc tgcagagttt caggattctg ataaggataa tgtagtttca cagctctacg
      481 agcctggaga atgtgtggac cctaattccc tcatcatccc ctacagaaca tctgaccccc
      541 ggtctgaaaa aatcgagtgg ataacagctc tggatgcaag aacggataaa aaaagtctta
      601 tacccgctaa tgctgtctac catccctacc atccccagag aggggttcat cttttcaagt
      661 ccaacaccaa tggcctagca tcaggaaacg tgctggaaga ggcagttttt catggattaa
      721 tggaagtagt ggaaagagat gcctggagca tttttgaagc ccgaaaaaaa cataaaaaag
      781 aagtaatatg tgataaaacc gaaaacaaac ttatcaacac cattttaact aaattcaggg
      841 aaaaaggagt ggatattaaa ctggtggatc tcactgccga tataaaaatt accaccatag
      901 cggcagtggc tgatgataaa gtactcaaag atccggctct tctaaccatg ggggtgggca
      961 cacatctaga tccagaaata gctgtaataa gggccttaac tgaagtggca cagagtcggg
     1021 ccacccagat acatggaacc cgggaggaca ccacccgggc gattttcatg cgaaaagcag
     1081 gatacgaacg tatgaaacgt attaacaaac actggtttgg tgattataaa gaaaaaatta
     1141 agctatccca gttgaaaaac aagtccacca catcctttaa ggatgacttg gaaatttccc
     1201 tggatgaact taagaaagta ggcatagaac agatttttta tgttgatatc acccgtccag
     1261 agattgatat tccagtagta agggttataa tacctggatt ggaagtgtac gctgttgatc
     1321 cagaacgaat gggaagaagg attatggata aggagatatc ttaatgtcct gctgtgtttt
     1381 ccatacaaaa ttcatgtacg gatgtatctt taccaactca acatagtttt atttaagaaa
     1441 tttttttcca ttatcgggct tttaatagct gtatatccaa tgtaataggg ttatgagata
     1501 ttgtttgaga aatttatgcc taaaaatcgc aaactataat aaatacatcc ccataattat
     1561 cttcatcaaa taatggggtg taacatgaaa agaagtattg ttttactatt tctggttatg
     1621 gttgcaacta ccttggcctc gggatgcaca tttgacaccg gaaacgaggt gtttaatgcc
     1681 aacggtgtga gctttgatta tccttcagga tgggataaac tatcccctga agatgtatca
     1741 atcggcacct c
//
LOCUS       DAOQVW010000357         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_90070_length_1750_cov_1.987611, whole genome shotgun sequence.
ACCESSION   DAOQVW010000357 DAOQVW010000000
VERSION     DAOQVW010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90070_length_1750_cov_1.987611"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKK85_07395"
     CDS             <1..211
                     /locus_tag="PKK85_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNS25878.1"
                     /translation="KLYKAPVTRTAVEKIGLKIVANIVMIGAITRMTKVISEEAARQA
                     VAESVPPGTEKKNLEAFEAGAELAG"
     gene            260..1366
                     /gene="sucC"
                     /locus_tag="PKK85_07400"
     CDS             260..1366
                     /gene="sucC"
                     /locus_tag="PKK85_07400"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNS25879.1"
                     /translation="MKLFEYQAKDLFKKDGIPVPKSRLAKTAEEAEKIAAEMNIPLAL
                     KSQVLVGGRGKAGGIKFADNPSQAKKLVQDLLKLEIKGERVDSILLEEKLNIESEFYV
                     SVVLDRSAKKPLIMASTQGGMDIEQVARETPEKLIKYHVNPLEEFLPYQAREIAIKMG
                     VPNPLISKVGGFIWKLFTVFRKYDATIAEINPLVVTPEEVIAADGKFEIDDDALYRQR
                     DLVGLAAEKKDEFAYVKLEGDIAVIGNGAGLTLSGMDMLKLYGGKPATFLDIGGGASQ
                     ENIARALNLVISNRQVKVVFLNVLGGITRADDVANGVVKVLKESDREVPLVIRLTGTN
                     EEEGQRILTEAGVSFETSMEAAAEKAVEIAENLN"
     gene            complement(1438..>1750)
                     /locus_tag="PKK85_07405"
     CDS             complement(1438..>1750)
                     /locus_tag="PKK85_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HNS25880.1"
                     /translation="DEIANLFEKVQTEVQNQNITNINDIKVIVINVANQMNMNLTDQQ
                     AEQIASSLSNAQQAQGSLDQFKSSLQNATQQASESQGFLNQIWNYLDSLWTQLQGLVG
                     Q"
BASE COUNT          525 a          357 c          457 g          411 t
ORIGIN      
        1 aaagctttac aaagctccgg tgaccaggac ggccgttgaa aaaatagggc tgaagatagt
       61 agccaacata gtgatgattg gggccatcac caggatgacc aaggtcatat ctgaagaagc
      121 agcccgtcag gcggtggcag aaagcgtgcc tcctggaact gaaaagaaaa acttggaggc
      181 ctttgaggcc ggagcggaac tggcaggatg aaataggcgt aaaaggctcg acatgaagac
      241 gtcaagggag acataaatca tgaaactatt cgaataccag gcaaaggatc tgtttaaaaa
      301 ggatggtata cctgtaccta aaagcaggct tgcaaagaca gcagaagaag cagaaaaaat
      361 agccgcagaa atgaacatac ctttagccct taaatcccag gttttagtag gtggaagagg
      421 aaaagcaggc gggataaaat tcgcagacaa cccatcccag gcgaaaaaac tagtacaaga
      481 tctattaaaa ctggagataa agggggaacg agttgatagt atattattag aggagaaact
      541 caacatagaa tccgaattct atgtaagtgt ggtcctggat cgctcagcta aaaaacccct
      601 gataatggcc agcacccagg gcggtatgga catcgagcag gtcgccaggg aaacccctga
      661 aaaactgata aaatatcatg tgaatccctt ggaagagttc cttccctacc aggccaggga
      721 gatagccatt aaaatgggcg tccctaaccc attaatatcc aaggtggggg gtttcatctg
      781 gaaattattc acggtcttca ggaaatatga tgctaccata gccgagataa atcccctggt
      841 agttacacca gaggaggtta tcgccgccga tggtaaattc gaaatagatg atgatgcact
      901 ctacaggcag agagacctgg tcggcctggc agctgagaag aaggatgaat ttgcctatgt
      961 gaaactcgag ggggacattg ctgtaattgg aaatggagca ggactcaccc tgagcgggat
     1021 ggatatgctt aaactatacg gtggaaagcc tgccacattt ttagacatag gtgggggagc
     1081 atcccaggaa aacatagctc gcgcacttaa cctggtcatc tccaaccgac aggtaaaggt
     1141 ggttttcctc aacgtcttag ggggtataac ccgggccgat gatgtggcca atggagtggt
     1201 taaggtactt aaagaatccg accgtgaagt cccgttggtt atccggctta ccgggaccaa
     1261 tgaagaagaa ggacaacgta tcctcactga agctggtgta tccttcgaaa cttccatgga
     1321 agccgcggca gagaaggcag tggagattgc tgagaattta aattaacgaa ttgattttga
     1381 ttagaaataa aaaaatagaa gagtggaaac ccgtaggttt ccaacattag ttttatttta
     1441 ttgccccact aaaccctgca gttgtgtcca taaactatcc agataattcc agatctggtt
     1501 aaggaaacct tgagactcac tggcttgctg ggtagcattt tgcaggctgc ttttgaactg
     1561 atccaggctt ccctgtgcct gctgggcgtt ggagagtgag ctggctattt gttctgcctg
     1621 ctggtctgtg aggttcatgt tcatctggtt ggccacgttt atcacgatga ccttgatatc
     1681 gtttatgttg gttatgttct ggttttgcac ctcagtctgc accttctcaa agaggttggc
     1741 tatctcgtca
//
LOCUS       DAOQVW010000358         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_90229_length_1748_cov_2.248080, whole genome shotgun sequence.
ACCESSION   DAOQVW010000358 DAOQVW010000000
VERSION     DAOQVW010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90229_length_1748_cov_2.248080"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PKK85_07410"
     CDS             complement(<1..150)
                     /locus_tag="PKK85_07410"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNS25881.1"
                     /translation="MKVTDFLGKRVVDRKAIEIGKVDDLIIEPETAMIKGIVISTGDF
                     GLRRTD"
     gene            322..1380
                     /locus_tag="PKK85_07415"
     CDS             322..1380
                     /locus_tag="PKK85_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS25882.1"
                     /translation="MKYSLKIFTIFGIPVELHFSFLLLMLAIYLVAVLNLIPGVDVVV
                     AVLITLLFVAVVLHELTHSYVARRYGVEIERIVLLPIGGVSAMKELPRDPHQELMIAV
                     AGPLVNLVLAVVLYPVFLLFGNSLSPSLNFLLYYFILMNFLLGAFNLLPAFPMDGGRV
                     LRAYLAERMNFIKATETAASIGKQLAIIMAIIGIFFNPFLILIAIFVYLGADQESKMV
                     LLNTLLEGINVRDIMTRGVKTVNPTDTVDEVLKVMFQHRHMGYPVYKDGELLGIITFH
                     DLSPIGEDKKHLPVEEFMTRDVLVTGPDEDVATVLEKLNLNNVGRLPVVQDGKLVGII
                     SKTDIMKALEMRKGSMSS"
     gene            1488..>1748
                     /locus_tag="PKK85_07420"
     CDS             1488..>1748
                     /locus_tag="PKK85_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNS25883.1"
                     /translation="MQEAYRFIITEILEGRIKNNRDLEKAKHRACREFDLPQFISNAE
                     ILENASPEERARIAPIIKKKPTRTLSGVAVVAVMVHSHQCPHG"
BASE COUNT          502 a          360 c          380 g          506 t
ORIGIN      
        1 atcagttctt cttaaaccaa agtcaccagt agatattacg attcccttaa tcatggctgt
       61 ttcgggttct ataattagat cgtcaacctt accaatttcg atggccttcc tatccaccac
      121 tcttttacct aaaaaatctg ttaccttcat attctgcacc attttatatt ctgtttttgt
      181 agttatatat tttttattaa ctatttctta atatttataa tctttttaag tctatatcag
      241 tagaaattac ctgataaaaa aggagatatc aaattaaggc tatgaaataa attattacaa
      301 acattaaaca tatagtacac catgaaatat tcacttaaaa tcttcaccat attcggaata
      361 cccgtggaat tacatttctc cttcctgctc ctgatgttgg ccatctattt agtggcggtt
      421 ttgaatttaa tacccggtgt ggatgtggtg gtggccgtac tgattactct tctcttcgtg
      481 gctgtggtgc tgcatgagtt aacccactcc tacgtggccc ggaggtacgg ggtggaaatt
      541 gaaagaatag ttttactccc cataggtgga gtatcggcca tgaaagaact tcccagggat
      601 cctcaccagg agttgatgat agcggtggcc ggacccctgg taaacctggt cctggcagtg
      661 gtgttgtatc ctgtattcct cctcttcggt aactctttat cccccagctt gaacttcttg
      721 ttgtactact tcatcctgat gaacttcctc ctgggtgcgt tcaatttatt accagcattc
      781 cctatggatg gtgggagagt tttaagggca tacttagctg agaggatgaa ttttattaag
      841 gcgactgaaa ctgcagcgtc catcggcaaa cagttagcca taatcatggc aattataggc
      901 atatttttca accccttcct gatcttaatc gccatattcg tctatttagg ggcggatcag
      961 gaatccaaga tggtcttgtt aaacacactc ctggagggga taaatgtcag agacatcatg
     1021 accaggggag tgaaaacagt gaaccccacc gacacggtgg atgaggttct gaaggtcatg
     1081 tttcaacata ggcacatggg atatccagtg tataaggatg gagagctcct gggcatcatc
     1141 accttccatg atctatcccc gattggtgaa gataagaaac atctgccagt agaagagttc
     1201 atgaccaggg atgtactggt aaccggcccg gatgaggatg tggccactgt actggagaaa
     1261 ttgaacctaa acaacgtggg ccgtttgccg gtggttcaag atggtaagtt agtggggata
     1321 atatccaaga cagatatcat gaaggccctg gaaatgagaa agggcagtat gagctcttga
     1381 accaattttt tttaaaagat atattatata aacgagaaat caatatcaac taattcgata
     1441 ccactggaat tatttctaat taacttgaaa tggagttttt atataatatg caagaggcct
     1501 acagatttat aatcactgaa atactggaag gaaggatcaa gaacaaccgg gatctggaaa
     1561 aggccaagca cagggcctgc cgggagttcg acctccccca gtttataagt aacgcggaga
     1621 tactggaaaa cgcatctcca gaagagagag ccaggatagc ccctataata aagaaaaaac
     1681 caacccgtac actttctggt gtggcggtgg ttgcggtgat ggtccattcc catcagtgcc
     1741 ctcatgga
//
LOCUS       DAOQVW010000359         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_90369_length_1746_cov_3.131875, whole genome shotgun sequence.
ACCESSION   DAOQVW010000359 DAOQVW010000000
VERSION     DAOQVW010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90369_length_1746_cov_3.131875"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKK85_07425"
     CDS             complement(<1..727)
                     /locus_tag="PKK85_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNS25884.1"
                     /translation="MNQKRQHYLLSRAEEACQLLEKHLDRDHVVRVISHNDADGISAA
                     GVICNAIAKCKGKFHVTIVPRLRDDILERLSREKYKLFFFCDMGSAYVERVSKLRGDS
                     IIADHHQTMDATGDEHENLVHVNPHLFGMDGTRDVSASGVTYLTVRSMDNPELTGMAL
                     AGAMGDMQGQNGFQGVNQTILQEGQDAGVIEVYEDLKLAYKEEEPLYKALSYTFNPAL
                     SGVSGDLEGSQGFLERLGLSYGIK"
     gene            complement(693..1094)
                     /locus_tag="PKK85_07430"
     CDS             complement(693..1094)
                     /locus_tag="PKK85_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNS25885.1"
                     /translation="MAMEPDWIEYSTEEIEELILKLNKEGNSASKIGVILRDQYGIPD
                     VKVVTGMKITKILEKHDQALEYPEDLMNLIRKAVNVRDHLEENPKDLHTRRGLQLMES
                     RIRRLVKYYVREGVLPQGWRYEPKKAALLVK"
     gene            complement(1096..1680)
                     /locus_tag="PKK85_07435"
     CDS             complement(1096..1680)
                     /locus_tag="PKK85_07435"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HNS25886.1"
                     /translation="MLKESQSQRTITFITGNQHKVKEAQGIFNHFQIEMEHVDLGYPE
                     IQGELDEVARYGARYAAKRLDKPVIVEDAGLFIRDLGWFPGTYSAYVQSTLKNRGILK
                     LMEDVQDRYAEFRSVVGFATPKAEPEIFLGVVEGKIIHEERGEYGFAYDPIFVPNGYD
                     KTFGELKIGIKNRFSHRRRSLEKFALWFEDYRGD"
BASE COUNT          429 a          448 c          347 g          522 t
ORIGIN      
        1 atttaatccc ataggaaagt cctagcctct ctaaaaatcc ctgactaccc tccaagtcgc
       61 cggaaacacc tgaaagggca ggattgaagg tgtaagacag ggccttgtac agtggctctt
      121 cttccttgta ggccagcttc agatcttcgt atacctctat aacacccgca tcctgtcctt
      181 cctgcaggat ggtctgattc acaccctgaa atccgttctg tccctgcata tcacccatgg
      241 ctccagccag ggccattcca gttaattcgg gattatccat ggaacgaact gttagataag
      301 tgacgcctga ggcgcttacg tcacgggtgc catccattcc aaataaatgg ggattaacat
      361 gtaccaggtt ttcatgttca tctccagtgg catccatggt ctggtgatga tcggcgatta
      421 tggagtcccc ccggagttta ctcacacgct ccacataggc acttcccata tcacagaaaa
      481 agaacaactt atatttttcc cggctgagac gttctaaaat atcatctcta agtcggggaa
      541 cgatggtaac atgaaattta cccttacact tggctatagc gttgcaaatt actccagcag
      601 cggagattcc atcggcatca ttatgggaga ttaccctcac cacatgatcc cgatctagat
      661 gtttctccag aagctggcag gcttcttctg ccctacttaa caagtagtgc tgcctttttt
      721 ggttcatatc tccatccttg aggtagcaca ccttcacgca cgtagtactt caccagtcta
      781 cgaatccggg attccataag ttgcaagcca cggcgggtgt gcagatcttt ggggttttct
      841 tccaagtggt ccctgacgtt gacggcttta cgaatgaggt tcatcaggtc ctccggatat
      901 tccagggcct ggtcgtgttt ttccaggatc ttggtgatct tcataccagt taccactttc
      961 acatctggaa ttccgtactg gtccctcagt attacaccaa ttttactggc ggagtttcct
     1021 tccttgttta atttaagtat cagttcttct atctcttcag tagagtattc tatccaatct
     1081 ggctccatag ccatcttaat cacctctgta atcttcaaac catagggcaa atttttctaa
     1141 agatcgtctt ctatgggaaa aacgattttt aatcccaatt ttaagctcac caaaggtttt
     1201 atcatatcca ttgggcacaa aaataggatc gtaagcgaat ccatattctc cgcgctcttc
     1261 atggattatc ttcccctcca ctacacctaa aaaaatctcg ggctcggcct tgggtgtggc
     1321 aaacccaacc accgacctga actcggcgta gcggtcctgg acatcttcca tgagctttaa
     1381 aatacccctg tttttcaggg tgctttgcac gtaggcggag taggttccag ggaaccatcc
     1441 caaatcccgg ataaacaagc cggcatcttc tacaatcact ggtttatcta ggcgtttagc
     1501 ggcgtaacga gcaccatacc gtgctacttc atctaattcg ccctgaattt cgggataacc
     1561 caagtctacg tgctccattt cgatctggaa gtggttgaat atcccttggg cttcttttac
     1621 tttgtgttgg ttcccagtta taaaggttat ggttctctga ctctggcttt ctttaagcat
     1681 ataaaaacca ctgttgcatt gaatatggat ttaaacaata aattcgtact ttatgcttaa
     1741 ttcatt
//
LOCUS       DAOQVW010000360         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_91037_length_1738_cov_2.767677, whole genome shotgun sequence.
ACCESSION   DAOQVW010000360 DAOQVW010000000
VERSION     DAOQVW010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91037_length_1738_cov_2.767677"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PKK85_07440"
     CDS             complement(<1..141)
                     /locus_tag="PKK85_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25887.1"
                     /translation="MFLTTKECKGSGECIEECPTGAIRLINGKAFSCITCGVCAEVCP
                     NRA"
     gene            complement(257..544)
                     /locus_tag="PKK85_07445"
     CDS             complement(257..544)
                     /locus_tag="PKK85_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-converting hydrogenase B subunit J"
                     /protein_id="HNS25888.1"
                     /translation="MIIYAGPLILGFLLGFVMGTRIQEDPESNLKFGASVYAVFFIMA
                     VIVAYFLGPFPYYYDVPLASGFVACALGIIMGKLIFGRKAPLKMEESATLE"
     gene            complement(541..1020)
                     /locus_tag="PKK85_07450"
     CDS             complement(541..1020)
                     /locus_tag="PKK85_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNS25889.1"
                     /translation="MSTILKIFVFPAAMIIMCLGVLTILGGHITPGGGFQGGAMIAAG
                     FIFCLIVYGLKESPFKLSHDFLAAVESVGALGFVFLGLVGLIFSGFFLYNVGVDLYSY
                     VPASIQSAFNYPDPTSAGIIPYLNFVVGLKVMVGLSAVVIAFLGFKELEKDKKEVQE"
     gene            complement(1021..1299)
                     /locus_tag="PKK85_07455"
     CDS             complement(1021..1299)
                     /locus_tag="PKK85_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EhbH"
                     /protein_id="HNS25890.1"
                     /translation="MSEGIKNLIMGFSLVIFTVALFQSIYDFKPLIYPGISYLYNWVG
                     TEIAPNMVTIVVFDWRGYDTLGEALILVTAVVAVLLVFGRGKVQMGGK"
     gene            complement(1292..1627)
                     /locus_tag="PKK85_07460"
     CDS             complement(1292..1627)
                     /locus_tag="PKK85_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-converting hydrogenase B subunit G, EhbG"
                     /protein_id="HNS25891.1"
                     /translation="MNIYDLIVKRIKEAQKADSDGPVTNVSTSAMLTAEITLISSVLV
                     TLIMLRLVNNVLMMVAVLVVLFTAVLAMPIMPQLKREMNDSLYAMTFYMVLALVIVIT
                     LFYWGNFNV"
BASE COUNT          516 a          408 c          344 g          470 t
ORIGIN      
        1 ggcccggtta ggacatactt cggcacatac accacaggtt atacagctga aggccttgcc
       61 attaatgaga cggatagcac cagtagggca ctcttcaatg cactccccgc tgcctttaca
      121 ttctttggtt gttaaaaaca tgggtgatcc ttcccttatt cctccaattt tgcttgatct
      181 tttattgatc tttatttaat cctttctaat cttatttcat gatttgattg ttagtaatat
      241 gtatgatgcg tggatatcat tccagagtag ctgattcttc catttttagg ggagcttttc
      301 tgccgaatat cagtttcccc atgatgattc ccagtgcaca agctacaaag ccactggcta
      361 ggggtacatc atagtagtat ggaaacggtc ccaggaagta agcaacaata actgccatta
      421 taaagaacac tgcgtaaaca gatgcaccaa atttaaggtt actctctgga tcttcctgaa
      481 tcctagttcc cattacgaag cccaggagaa atcctaaaat aaggggtcct gcatatatta
      541 tcattcctga acctcctttt tatccttttc tagttcctta aatcccagga atgctattac
      601 cacagcgctt aatcccacca tgaccttcaa accaactacg aagtttaggt atggaattat
      661 tccagcagag gtgggatccg ggtagttaaa ggcggactgg atagaggcag gcacgtaact
      721 gtaaagatcc acacccacgt tgtacaggaa gaatcctgag aatattagtc ccaccaaccc
      781 caggaaaaca aatcccaatg caccgacact ttcaactgct gctaaaaaat catgagaaag
      841 cttaaatgga ctttccttaa gaccgtagac tattaaacag aatatgaatc ctgcagcgat
      901 catggctccg ccctggaacc ccccacctgg tgtgatatgg ccacctagga tggtgagaac
      961 ccctagacac attataatca tggccgcagg gaatacaaat atcttgagaa tggtgctcat
     1021 ctatttacct cccatttgga ctttccctct tccaaagacc agtaaaactg caactacggc
     1081 agttacaaga attaaagctt cacccagggt atcgtaacct ctccagtcaa atactacgat
     1141 ggttaccatg ttgggtgcga tttcagtacc tacccagttg tacaggtagc ttataccagg
     1201 atatataagc ggtttaaagt cgtatattga ttggaataat gctactgtaa atattaccag
     1261 ggaaaatccc attatcaaat tcttaattcc ttcagacatt aaaattcccc caatagaaca
     1321 gggtaataac tataaccagg gccaaaacca tgtaaaaggt catggcatat agagagtcat
     1381 tcatttctct tttcaactgg ggcataatag gcatggccaa caccgcggtg aatagcacca
     1441 ccaggactgc caccatcatc agcacattat taaccaatct caacatgatc agagtcacta
     1501 gtactgagga tatgagggtg atttccgcgg ttaacatggc cgaggtggac acattagtca
     1561 ctggtccatc tgaatcagct ttttgagctt cttttatcct ttttacaatc aagtcataga
     1621 tgttcatgga acaccccctg caaggctagt tgatatcccc tgcagatagt tggtggccat
     1681 ttgggggaac agacccagga gcagacagac caggagcagt atggacaggg ccaggata
//
LOCUS       DAOQVW010000361         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_91408_length_1734_cov_2.516379, whole genome shotgun sequence.
ACCESSION   DAOQVW010000361 DAOQVW010000000
VERSION     DAOQVW010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91408_length_1734_cov_2.516379"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..997
                     /gene="cas1b"
                     /locus_tag="PKK85_07465"
     CDS             29..997
                     /gene="cas1b"
                     /locus_tag="PKK85_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HNS25892.1"
                     /translation="MAKKNYYLMSDGILKRRENTVYFVNKDGKRPLPINQIYSIYAYG
                     SLTFSSQVVHLLAKEGVPIHFFNYYGFYDGSFYPRETLLSGNLLIKQAEHYMDHDKRM
                     VLAKKFVEGASKNMEKVVAYYKIDNQISHTLKDLDYSQTITDVMNVEARIRSHYYTLM
                     DEILPNGFKMEGRSRKPPENMVNALISFGNSLVYSTVLSEIYHTQLNPTISYLHEPFE
                     RRYSLALDLSEIFKPFLVDRLIFYLVNKRMLSEDDFEQDLNYCLLNEGGKKIFLKEYD
                     ERLKKTIKHRELNRKVSYRRLIRLEAYKLTKHLLGSKEYNPFVMWW"
     gene            1000..1263
                     /gene="cas2"
                     /locus_tag="PKK85_07470"
     CDS             1000..1263
                     /gene="cas2"
                     /locus_tag="PKK85_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNS25893.1"
                     /translation="MYLIIVYDIKVERVNKVKGFLRTHLNWIQNSVFEGEVTQSEMEI
                     IKKGLNKIIKKSEDSVIIYRFRSDKAFERKVMGIEKAPINAII"
BASE COUNT          580 a          300 c          323 g          531 t
ORIGIN      
        1 ttatgagttg tgttgggtgt gatgaaaagt ggctaagaaa aattactatt taatgtccga
       61 cggtatctta aaaagaagag aaaacacggt ctactttgtg aacaaggatg gtaaaaggcc
      121 tttacccatc aatcagatat attccatcta tgcttatggt tcgttaactt tttcctccca
      181 agtagttcat cttttagcca aagaaggggt tccaattcat tttttcaact actacggctt
      241 ttatgatggt agtttctatc ctagagaaac tttattatcg ggaaaccttt tgattaaaca
      301 ggccgaacac tacatggacc atgataaaag aatggtcctg gccaagaagt ttgtggaggg
      361 agctagtaag aacatggaga aggtggtggc ttattacaag atagacaacc agataagcca
      421 cactctaaaa gatcttgatt attcccaaac cataaccgac gttatgaatg ttgaagcccg
      481 tatacgttcc cattattaca cattaatgga cgaaattctt cccaatggat ttaaaatgga
      541 aggcagatcc cgaaaacctc cagagaacat ggtcaatgca ctaataagtt ttggaaactc
      601 tttagtttac tccacggtct tgtcggaaat ctaccacact caactcaatc ccaccatatc
      661 ttatctccac gaaccttttg agcgtagata ttctctggcc ctggatttaa gtgaaatatt
      721 caagccattc ctggttgata ggctaatctt ctacttggta aataagcgaa tgctcagtga
      781 agacgatttt gaacaggacc tcaactactg tctcctgaat gagggtggaa agaaaatttt
      841 cctaaaagag tacgatgaac gtctcaaaaa gaccataaaa catcgtgagc tcaatcgtaa
      901 agtttcttac aggcgtttaa tacgtttgga agcctataaa cttactaaac atcttctggg
      961 atctaaggag tataatccct ttgtgatgtg gtggtgaaca tgtatcttat aattgtctac
     1021 gatatcaaag tggagagagt caacaaagtc aaaggattcc tccgtacaca tctcaactgg
     1081 atccaaaact ctgtatttga aggagaagta actcaaagtg agatggaaat cataaaaaag
     1141 ggattaaata agataataaa gaaatctgag gattcagtga tcatttaccg cttccgtagt
     1201 gacaaagcat ttgaaaggaa agtgatgggg attgaaaaag cccctatcaa tgctatcatc
     1261 taattataat tatctttttt taatttaaga gatattgatg tctaactaac tgtttccttc
     1321 aattttctgc agatcgtcta atcctcatta ctaatgtgat cattattatt actctaaatt
     1381 aaatcttctc cattagttta aatgggggta aatatagctt caggagccta tgatcctttt
     1441 taaaaaatat agatctggtg tttatataat ctaaagattt tatttttcgc aaataaaaat
     1501 gaataaagtg tcgtcaaacc atataaagaa attccaactt tatagatcaa caattttaag
     1561 tgtttgaggg gccagattat atggtgtatt agggaaaata gggcaaattt cagccctact
     1621 aaaatcagac cattttggga ttgaaatgtg actattaact tgacattgcc ggctatgggt
     1681 agtactaaaa tcagaccatt ttgggattga aattaagtcc agtactgttt aacc
//
LOCUS       DAOQVW010000362         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_91448_length_1733_cov_3.628129, whole genome shotgun sequence.
ACCESSION   DAOQVW010000362 DAOQVW010000000
VERSION     DAOQVW010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91448_length_1733_cov_3.628129"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKK85_07475"
     CDS             complement(<1..349)
                     /locus_tag="PKK85_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNS25894.1"
                     /translation="MKVVVFDNSGTLIERYRAIKDLRTGIICDDVSSIDLVDMVPNRA
                     LVVLQTDPAQCLANAISDQTIYHFLKKNNVKFDISYSSGDVLREQVWETIKNDGYAQI
                     MDVQDTITTVIEKK"
     gene            complement(424..1056)
                     /locus_tag="PKK85_07480"
     CDS             complement(424..1056)
                     /locus_tag="PKK85_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNS25895.1"
                     /translation="MVEIFLFGVSSFLVGLSGALVPGPMLTVTIANSLKNGPLAGPMV
                     VSGHMIAEVAILLLLVLGLGWLIGSATATLVIGTIGGMVLVYMGYRITRSAPPSLEGK
                     VEAKDKYGSVLSGVLSSISNPYFFIWWATIGWAFVVKGLEFFGLLGLLGFMAGHWTAD
                     MGFYSLVSVFSSRGSEILTQKYYKTLMYGCGIFMIVLGIYFVFSAQIRAL"
     gene            complement(1195..>1733)
                     /locus_tag="PKK85_07485"
     CDS             complement(1195..>1733)
                     /locus_tag="PKK85_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513973.1"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNS25896.1"
                     /translation="EVMKIEKAKVYCRTCSTRTKFARNATATYGFEVEAVKSPKEAVK
                     DVDVVVTTTPSRKPLIKVDWISSGTHINAMGADAPSKQELDTNLLQKSKIVVDSWEQA
                     KHSGEINVPVSQGILKRNDIHAKLGEIIIGKKVGREKDEITIFDSTGLAVQDTVTAWQ
                     IYRKVLEKGVGTKFDFQG"
BASE COUNT          486 a          414 c          317 g          516 t
ORIGIN      
        1 attttttctc aatcactgta gtaatggtgt cctgaacatc cataatctgg gcgtaaccat
       61 cattttttat ggtttcccaa acttgttccc ttaaaacatc ccctgaagaa taactgatgt
      121 caaatttcac attatttttt tttaaaaaat ggtaaatggt ttgatcacta atggcgttgg
      181 ctaaacactg ggcgggatct gtttgcagta ccaccagtgc ccggttaggt accatgtcca
      241 ctaggtcaat ggaactcaca tcgtcacaaa taattccggt tctaagatct ttaattgctc
      301 ggtatctttc aattagagtc ccagaattgt caaaaacaac taccttcatt atattccacc
      361 ttagcacctt gttcctatta aatgttgtga ataaagtaat tttcaagata aaaatcttta
      421 taattacagg gcccgtatct gggcactgaa cacgaaataa attcctaaaa ctatcatgaa
      481 aattccacaa ccgtacatca gtgttttata atatttttga gttaaaattt cagagccccg
      541 gctgctgaag acggatacta aactataaaa ccccatatct gctgtccagt gcccagccat
      601 aaatcctaaa agccctaata gaccaaaaaa ttccaagccc ttgaccacaa atgcccagcc
      661 aatagtcgcc caccagatga aaaagtaggg gttggatatg ctgctaagaa ccccactcag
      721 gaccgatccg tacttatcct tggcttccac cttaccttct aaactgggtg gtgctgatcg
      781 tgtgatacga taccccatgt aaactagaac cattccccca atggttccga ttaccaaggt
      841 ggctgtggcg gatccaatta accatcctag tcccagaacc aacaacaata aaattgccac
      901 ctcggcgatc atatgacccg aaacaaccat tgggcctgca agaggtccat ttttcagtga
      961 attggcaatg gtaactgtta acatggggcc gggaaccaga gcaccagata ggccaaccag
     1021 aaatgagctc accccaaata aaaatatttc caccatcaat ttaaccacag ttaaaaaaat
     1081 ttaacgatga aggaggttga tgaattatga ttggagggga tgcttattaa atcattgccc
     1141 ttccttatac cccctggtgt tctgttaatg gaatgttttc tgcttaactg tccattatcc
     1201 ttgaaaatca aatttagtac ccactccttt ctccaagacc tttctgtata tctgccaggc
     1261 ggtaaccgta tcctgtactg ccaggccagt ggaatcaaag atggtgattt catctttttc
     1321 tctaccaacc tttttcccga tgatgatttc tcccagcttt gcgtgtatat cgttccgttt
     1381 taaaataccc tgtgaaacag ggacgtttat ttctccactg tgcttggcct gctcccagga
     1441 atctactaca attttagatt tttgcagcaa gttggtatcc aactcctgtt tactgggcgc
     1501 atcagccccc atggcattga tgtgggtccc ggaactgatc caatccactt tgatcagtgg
     1561 cttccgggat ggggtggttg ttacaaccac atcaacatct ttcacggctt ctttcggtga
     1621 tttaactgct tccacttcaa aaccgtaggt tgcagtggca tttctagcga acttggtacg
     1681 ggtgctgcag gttcggcagt aaactttggc tttttctatc ttcataactt cat
//
LOCUS       DAOQVW010000363         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_92690_length_1719_cov_4.039663, whole genome shotgun sequence.
ACCESSION   DAOQVW010000363 DAOQVW010000000
VERSION     DAOQVW010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92690_length_1719_cov_4.039663"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1719)
                     /locus_tag="PKK85_07490"
     CDS             complement(<1..>1719)
                     /locus_tag="PKK85_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNS25897.1"
                     /translation="ALVAAERLKDFPNSKVLVVAPSKPLAVQHEESFHQFLKVPTTVL
                     TGAVNPVERSKRWDEAQVILATPQTIESDLIGGRYSLDGVSLLVFDECHRSTGSYAYV
                     FLANNYQTTAKNPLILGLTASPGGDEEKIKAVCKNLFIQDVVVKNEGDQDVKPYFNPI
                     EVDWVRVDLTPEQKAVKSHLEAALKGRLKGLKKMGVIRSISQISKRDILKARNRVQNR
                     ISRSTQPPRNCLIAISILTAVFSILHSLELLETQGIRNLNGYFQRLGKKNTKAAKGLL
                     KDGEFKIAVYLTKKALEEGIEHPKMQELMKILKEELQEDRQIIVFSQYRDTVNEIYNK
                     CQEEGVKAVKFFGQASREKEKGLSQKEQKEIIKAFRMKTYQVLVSTSVAEEGIDIPSV
                     DLVILYEPVPSEIRMIQRRGRTGRKSKGRMIILITKGTRDESYYYSSQYKERRMKNQL
                     SQDYEKKVEIAPVQVKLVSGAEKPTVYVDHREAKSGVARELHNLGVKVVTTSLSVADY
                     QVGNEVAVERKSTRDFVGSIIDKRLHKQAKDLVENFQRPVIILEGDDLYSGGLHPNAV
                     RGALASL"
BASE COUNT          410 a          435 c          331 g          543 t
ORIGIN      
        1 aggctggcca gcgccccacg aacagcgttg ggatgcagac ctccactgta aagatcatcg
       61 ccttccagga ttataaccgg tctctggaag ttttccacca gatccttagc ttgcttatgc
      121 agtcttttgt caatgattga tcccacaaag tcccgggtac ttttcctttc cacagccact
      181 tcattaccca cctggtaatc tgccactgac aggctggtgg ttactacttt aacccctaag
      241 ttatgcaatt cccgggcaac acctgatttg gcttcccggt gatcaacata taccgttggt
      301 ttttctgctc cggacactaa tttcacctgt accggagcga tctcgacttt cttttcataa
      361 tcctgggaga gttgattttt cattcttctc tccttgtact ggctggaata atagtaagac
      421 tcgtcacggg ttccttttgt tataagtatt atcattcgtc ctttactttt ccgtccagtc
      481 ctaccccttc tctggatcat cctgatttct gatggaacag gttcgtagag gatgaccaga
      541 tccacagagg gtatgtctat accctcctca gctacactgg tggacaccag cacctggtag
      601 gttttcatac ggaaagcctt tatgatctcc ttctgttctt tctggctcaa gcctttttct
      661 ttctcccggg aggcttggcc gaagaattta actgccttaa caccttcttc ctggcattta
      721 ttataaatct cgttgacagt gtcccggtac tggctgaaca ctataatctg ccggtcctct
      781 tgaagctctt ctttaagtat cttcattaac tcctgcattt taggatgttc aatcccttct
      841 tctagggcct ttttagttag ataaacagct attttaaact ctccatcttt taacaagcct
      901 ttagcggcct ttgtattttt tttacctagg cgctggaagt atccgtttaa attacggatc
      961 ccctgggttt ctagaagttc cagtgaatgt aagatactaa aaacagcagt taaaattgag
     1021 atagcaatta aacaattccg gggaggctga gtacttctgg atatcctgtt ttgtactctg
     1081 ttacgagcct ttaaaatgtc tcttttgctt atctgggaga tggatctaat gactcccatc
     1141 ttcttcaaac ccttcaatct tcctttcagc gcagcctcca agtggctttt tacggccttc
     1201 tgttcagggg tcagatccac ccgcacccaa tctacttcaa taggattaaa ataaggtttt
     1261 acatcctgat ctccctcatt tttcaccacc acatcctgga taaaaagatt cttacacacc
     1321 gcctttattt tttcttcatc tcccccaggt gaagctgtta aacccaaaat caaaggattc
     1381 ttagcggtgg tctggtaatt gttggccagg aatacgtagg cataggatcc tgtgctccgg
     1441 tggcattcat caaaaaccag caaagaaaca ccatcaagag agtaacgccc acctataaga
     1501 tcagattcaa tggtttgtgg cgtggctaag attacctgtg cctcatccca gcgctttgat
     1561 ctttcaactg gattcacggc acctgttaaa actgtagtgg gaacttttaa aaactggtgg
     1621 aaactttcct catgctgcac agctaggggt ttgctgggag ccaccaccag tactttactg
     1681 ttaggaaagt ctttcaaccg ctcggcagca acaagagca
//
LOCUS       DAOQVW010000364         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_92709_length_1719_cov_3.012019, whole genome shotgun sequence.
ACCESSION   DAOQVW010000364 DAOQVW010000000
VERSION     DAOQVW010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92709_length_1719_cov_3.012019"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /gene="nadA"
                     /locus_tag="PKK85_07495"
     CDS             complement(<1..243)
                     /gene="nadA"
                     /locus_tag="PKK85_07495"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNS25898.1"
                     /translation="MNQLQKEILKLKKQKNAIILAHNYQTAEIQEVADFLGDSLELCL
                     KASQIEDVDMVVFCGVDFMAETAYILNPDKKILLPDI"
     gene            complement(392..1153)
                     /gene="mtnP"
                     /locus_tag="PKK85_07500"
     CDS             complement(392..1153)
                     /gene="mtnP"
                     /locus_tag="PKK85_07500"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNS25899.1"
                     /translation="MLAVIGGTGIYRLVEEGEELETMVMNTPYGESPQITRFMINDTP
                     LFFMPRHHQGHDYPPHRINYRANLCALQKLGVKRILATNAVGSLDEKLKPGTFLAPDD
                     FLDFTHHRPLTFYDHRTVHVDVTQPYCPHLRRVLLDSGKVVDGGVYVCVEGPRFETPA
                     EIRMFHSLGGTVVGMTGLPEVVLARELSMCYASVCMVTNHAASISQDKVTIDEVFGIM
                     EGKKKELTTLLLESIQNTPLRRDCHCEQALQGAEI"
     gene            complement(1171..>1719)
                     /locus_tag="PKK85_07505"
     CDS             complement(1171..>1719)
                     /locus_tag="PKK85_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNS25900.1"
                     /translation="ILHWVRESFEQVFHRDSEDLDMGVVYDVAHNIAKREVHKIKGRD
                     TEVYVHRKGATRAFGPGRREIPSCYREVGQPVLLPGTMGTASYILHGTETAMEETFGS
                     TAHGAGRKMSRAGAKRTYHGEEVKKVLADRGIYIKATSMPIVAEEAPGAYKDVNVVVK
                     TVHETGISRLVAKMVPLGVAKG"
BASE COUNT          381 a          442 c          413 g          483 t
ORIGIN      
        1 gatgtcgggt agtaaaattt tcttatcagg gtttagtatg taggctgttt cagccatgaa
       61 gtccactcca cagaacacca ccatatccac gtcttcgatc tggctagcct taaggcagag
      121 ttccagggag tcccctagaa aatcagcaac ctcctgtatc tctgcagttt gataattatg
      181 agctaatata atggcatttt tctgtttttt aagttttagg atttcttttt gtagctgatt
      241 caacctaaat ctctcctaat taagataaat atgagtttat aaataaggtg actggaaaaa
      301 ttttggtact tggtatataa attgtctttt atgatcctag aaaagttatg gtgccagaat
      361 caggataccc ctgcatcgga taattatctc atcatatttc agcgccctgt aaggcctgtt
      421 cacagtggca atctctcctg agcggcgtgt tttggatgga ctctaagagt aaagtggtta
      481 actctttctt tttcccctcc atgatcccga agacctcgtc aatggtaacc ttgtcctgtg
      541 aaatggaagc cgcatggttg gttaccatgc acaccgaggc gtagcacatg gaaagttcac
      601 gggccaagac cacctcgggt aagcccgtca tacccactac cgttcctccc agactgtgga
      661 acatccttat ttccgccggg gtttcaaagc ggggtccctc cacacagacg tacactcctc
      721 catccaccac tttccccgag tccaaaagca cccttcgcag gtgggggcag tatggctggg
      781 taacatccac gtgtacagtg cgatgatcat aaaaggttaa tggtcggtgg tgggtgaaat
      841 ccaggaaatc gtcgggggcc aggaaggttc cgggttttaa tttttcatcc agggatccca
      901 cagcattggt ggccagtata cgcttcactc ccagtttttg aagggcgcag aggttggccc
      961 ggtagttgat acgatggggt gggtagtcat ggccctggtg gtgccggggc atgaagaaca
     1021 gtggtgtgtc atttatcata aagcgagtga tctggggtga ttcaccgtag ggtgtgttca
     1081 tcaccatggt ttccagttct tcgccctctt ccaccagccg gtagattcca gttcctccaa
     1141 taactgccag catgttccct gtcctgtact ttatcccttg gccactccca gtggcaccat
     1201 cttagccacc aaacgggata taccggtttc atggactgtt ttcactacta cattcacatc
     1261 tttatatgcc cctggagctt cttcagccac gatgggcata gaagttgcct ttatatatat
     1321 gccccggtca gcaaggacct tcttaacctc ttctccatgg taggtgcgct tagcacctgc
     1381 tcgactcatc ttgcggcctg ctccatgtgc agtggagccg aaagtttcct ccatggctgt
     1441 ttcggtgccg tggaggatgt aggatgcggt tcccatagtc cctggtaaga gtacgggttg
     1501 gcccacctcc cggtaacagg aggggatttc ccttcggcct ggtccgaagg cccgggtggc
     1561 tcccttacgg tgcacgtaaa cctcggtgtc ccggcccttg atcttatgca cctcccgctt
     1621 ggctatgttg tgggccacat cgtagaccac ccccatatcc aggtcttcac tgtcacggtg
     1681 aaatacttgt tcaaaacttt cccgtaccca gtgcaggat
//
LOCUS       DAOQVW010000365         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_92720_length_1719_cov_2.796875, whole genome shotgun sequence.
ACCESSION   DAOQVW010000365 DAOQVW010000000
VERSION     DAOQVW010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92720_length_1719_cov_2.796875"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PKK85_07510"
     CDS             complement(<1..1167)
                     /locus_tag="PKK85_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNS25901.1"
                     /translation="MYHKKHLIFLLPLLFISLFVFSSSNTSYGAELPDLTNTDIQAPD
                     SVYAGQSILVKITNWNTGAATSDDAGYKDTLYLSQDTAIDDSDQILYTNRQTAPREAN
                     TPLNLSIPVLIPADTAAGYYNLIFVTDSSKELSELDEDNNILSKGILIQRDTTPPLIN
                     SIDPAPGAFNVGSAKAIKVSFNEDIKTGNLWIDLVNGNGAKVAYTTAISSNTLTITPI
                     HPLPQSLYTLTLHPGAVTDQSGNPNSLIVSSFTVSIYRIDIIHYGTGGDIRKNKLVYK
                     NLPKTTLTNQILAAAKKGTPMVTFGDGSGPRVMVVAGVHGNEVPAVIAAMRLINNLSK
                     TSIKGTIYVVPFAIPSATARKSRYWKGANPNQVANKPGTPTNKIITLARQFNVNA"
     gene            complement(1408..>1719)
                     /locus_tag="PKK85_07515"
     CDS             complement(1408..>1719)
                     /locus_tag="PKK85_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNS25902.1"
                     /translation="RRTSIDELPQLFNILKGEMSLIGPRPERPLFVDEFRDQIPKYMI
                     KHHVRPGMTGWAQVNGYRGDTSIKRRIKYDIYYVENWTLGLDIRIFFRSLFHLVDENA
                     Y"
BASE COUNT          398 a          346 c          462 g          513 t
ORIGIN      
        1 ggcgttgaca ttgaactgcc gggccagtgt aatgattttg ttggtgggag tgcctggttt
       61 gttggcaact tggtttggat ttgcaccctt ccagtatctg cttttcctag ctgttgcaga
      121 tggtatggca aagggcacca cgtagatggt tcccttaatt gaagttttac tgaggttgtt
      181 tatcagtctc atggcagcta taactgctgg aacctcgttc ccatggacac cagccacaac
      241 cattacccta gggccgctgc cgtcaccaaa ggttaccatg ggggttcctt tctttgcagc
      301 ggcaaggatc tggttggtca gtgtagtttt ggggagattt ttgtacacca gtttattctt
      361 cctgatatct cctccagtcc cgtagtggat gatatcaatt ctgtagatac tcacagtgaa
      421 gctgctaact attaacgagt tgggattacc ggactggtca gttaccgctc cagggtggag
      481 ggttaaggtg tacagggatt gtggtaaggg atggatgggt gttatggtga gtgtgttgct
      541 ggatatagct gtggtgtagg ctaccttggc tccgttgccg tttaccaggt cgatccatag
      601 gttccctgtt ttaatgtcct cgttgaagct tactttgatg gccttggcag agcctacatt
      661 aaatgcacca ggggcaggat caatggagtt tataagggga ggggtggtgt ccctctggat
      721 gaggatgccc ttagacagta tgttgttatc ttcatccaac tcggagagtt ccttggatga
      781 atctgttaca aaaataaggt tataatatcc tgctgcagta tctgctggga tcagaacggg
      841 gatactcaga tttaatgggg tgtttgcttc ccgtggggcc gtctgtctat tggtgtataa
      901 aatctggtct gaatcatcta ttgcagtgtc ctgtgataag tagagggtgt ccttgtagcc
      961 tgcatcatca cttgttgcag caccagtatt ccaattagta atctttacga ggatgctttg
     1021 ccccgcgtaa acactatctg gggcctgtat atccgtgttt gttaggtctg gcagttcagc
     1081 accataggag gtgtttgaac tgctaaagac aaacaagctt atgaaaagta gtggaagtag
     1141 gaatataaga tgcttcttat ggtacatttt ttgttcatcc cccttgagaa tgataatata
     1201 ttttgtctag aatgtaatgg taatactagg actttattta atataatttt tttgtacaaa
     1261 acacaaaaaa aataggggga cagtggaagg gattctaaac agaggggagc accttagaag
     1321 atcctgtgga tgggggttcc tgaaaaagtt cctggcccat gttttctgat gcagagaatg
     1381 gggctcatcc aggggagtaa cccgagatca gtaggcgttc tcatccacca ggtgaaagag
     1441 gctgcggaag aagatcctta tatccaatcc cagggtccag ttctccacat aatatatatc
     1501 atacttgatc ctcctcttaa tggaggtatc accccggtaa ccgttgacct gggcccagcc
     1561 agtcatcccg ggtcggacat ggtgcttgat catgtacttg ggtatctggt cccggaattc
     1621 atccacgaag aggggtcgtt ctggtcgggg tccgatgagg ctcatctccc ccttcaggat
     1681 gttgaatagc tggggcagtt catcgatgct ggtccggcg
//
LOCUS       DAOQVW010000366         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_92733_length_1719_cov_2.571514, whole genome shotgun sequence.
ACCESSION   DAOQVW010000366 DAOQVW010000000
VERSION     DAOQVW010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92733_length_1719_cov_2.571514"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /gene="topA"
                     /locus_tag="PKK85_07520"
     CDS             complement(<1..1194)
                     /gene="topA"
                     /locus_tag="PKK85_07520"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HNS25903.1"
                     /translation="MHEVIICEKPKAAEKISQALPGYAEKKSYKRVPYYEIEKNGNKT
                     TILAAVGHLYSLSPLRKENGRIFEVEWVPLHTKDKSKGYVKNYIDAIVKFSKNADRFI
                     HACDYDIEGTLIGFNALKYACGDKSVEQAVRMKFSTLTEEDLLEAYHNPIDLDFHQVD
                     SGIARHVLDFLFGVNISKYLTDSVMAATSRYIQLSAGRVQTPTLSILVDREKQITEFK
                     PEPYWLIKADLGKGIIADHKEGKIFNKKKADNILAHCQGKDAVVHKISHRKNEQLPPV
                     PFDLGTLQSEAYAVFGFSPKKTQSIAQNLYTEGYTSYPRTSSQKLPASIGYQKILTKL
                     SKNSNFARHIRKLKKPLKPREGKKTDAAHPSIHPTGVLPSKLGRDYQKLYELIVHRFI
                     SVFGKN"
     gene            1365..>1719
                     /locus_tag="PKK85_07525"
     CDS             1365..>1719
                     /locus_tag="PKK85_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNS25904.1"
                     /translation="MLIGVISDTHIPERASKIPENVFEIFDGVDMILHAGDLVSLEIR
                     DILNTLAPTTCVQGNMDRYYGIKLPEREWVEVGGVKIGLNHGEVYPRGNTQQLRYIGL
                     EMGVDVLITGHTHYAF"
BASE COUNT          456 a          352 c          395 g          516 t
ORIGIN      
        1 attctttcca aaaacactaa taaaacgatg tactataagt tcgtagagtt tctgataatc
       61 tcgacccagt ttactgggta aaactccggt ggggtggatg gatgggtggg cagcatcagt
      121 tttcttacct tcacggggtt ttaaaggctt tttgagtttt cggatatgtc tggcgaagtt
      181 gctattttta ctgagtttgg tgagtatctt ctgataacct atgctggctg gtaacttctg
      241 ggatgaagta cggggatagg aggtgtatcc ttcagtgtat aaattctggg ctatggattg
      301 ggtttttttg gggctgaaac caaacacagc gtaggcttct gactggaggg ttcccaagtc
      361 aaagggtacc ggtggtaact gttcattttt tctatgactg attttatgca ccaccgcatc
      421 cttaccctgg caatgggcca gaatattatc tgcctttttt ttgttgaaga tctttccctc
      481 tttatgatcg gcaattattc ccttcccaag atcagccttt ataagccagt atggttcggg
      541 tttgaactct gttatctgtt tttcacggtc caccagtatg gacagggtag gggtctggac
      601 acggccagct gaaagctgaa tgtaacggga agtagccgcc attaccgaat cagtgaggta
      661 cttggaaata ttaactccaa agaggaaatc caggacgtgg cgagcaatcc cactgtccac
      721 ttggtgaaaa tcaaggtcaa tggggttatg gtaggcctcc aaaaggtctt cctcggttag
      781 tgtggagaac ttcatcctca ccgcttgttc cacactttta tcaccacaag catatttaag
      841 ggcgttgaaa ccgatgagtg ttccctcaat atcataatcg caagcatgta taaatctatc
      901 cgcattttta gagaacttca caatggcgtc aatgtagttt ttaacgtaac ccttggactt
      961 gtccttggtg tgcagaggta cccattccac ctcaaatatt cttccatttt cctttctaag
     1021 gggagataat gagtaaagat gtcctactgc agctagtatg gtggttttgt ttccgttttt
     1081 ctcaatttcg tagtatggga cccttttata actcttcttt tccgcatagc caggtagggc
     1141 ctgggatatc ttctcagcgg ccttgggctt ctcgcagata attacttcat gcataaaatc
     1201 actggaaaaa atattgtgta acaaaaactt agtatgttaa atagtatgcc aggtttttca
     1261 actatcagat atagtaagtt gacatatata taatactaag aatagtgcaa gctataaacc
     1321 tatcctgacc ttgatcatgc acggcagaga gtagaggaga taaaatgtta ataggagtta
     1381 tatccgacac ccacattcca gaaagggcct ctaaaattcc cgaaaatgtt tttgaaatat
     1441 ttgatggagt ggatatgata ttacacgccg gtgatctagt ttccctagaa atcagggata
     1501 tcctaaacac cctcgccccc accacctgtg tacaaggaaa tatggaccgc tactatggca
     1561 ttaagctccc tgagagggaa tgggtggaag taggaggggt taagattggc ctaaaccatg
     1621 gtgaagttta tccccgtggg aacacccagc agctccgcta cattggcctg gaaatggggg
     1681 tggatgtgct gataaccggt cacacccact atgccttca
//
LOCUS       DAOQVW010000367         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_92925_length_1717_cov_2.161853, whole genome shotgun sequence.
ACCESSION   DAOQVW010000367 DAOQVW010000000
VERSION     DAOQVW010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92925_length_1717_cov_2.161853"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1085)
                     /locus_tag="PKK85_07530"
     CDS             complement(207..1085)
                     /locus_tag="PKK85_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNS25905.1"
                     /translation="MPKFMIIGPLTKDRIVKRDNVCNTVGGGVYYQSAVLSGLGMDNT
                     VITTMSKMDKGLMKQFPGAAQIIPIYTEKTMEFENIYPDDNPNHRIQKTQIPVNPIKP
                     ANFKDLNLKAYDALLLSPLSPYDIPQETVQYLSKQDIPIYLGAQGYLRHVESGNVVLK
                     PWRDYKRFLKLVDFLFLDELEARVILGNSSDNCAEIAQVLSNFGPEEVIITRGDRGAV
                     IYSQITAENKNIHDIPSFPPEEIVDPTGLGDTFMAAYAVKKLETPDPAECGRFAAHIS
                     SLKMGQKGALFEIKRK"
     gene            complement(1151..>1717)
                     /locus_tag="PKK85_07535"
     CDS             complement(1151..>1717)
                     /locus_tag="PKK85_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNS25906.1"
                     /translation="DIAISLKREGFKRIYVADLDAIEGRGSNLEIVRKINHVLPVMLD
                     WGVKDIESFAFALDFADKIIIATETLTSWADLGEIFHKFPRQRMMVSIDVKDGQILSR
                     DPSLTFGEVKSILEKLKLEEIILLDISGVGTLSGFNLDLLNNFRGMEDALILGGGITP
                     EEVKNLNDRGVNKFLIGTALHSGKVGLQ"
BASE COUNT          455 a          362 c          337 g          563 t
ORIGIN      
        1 ttataactac aggaataatg gattccaata accgtcaaac tctaattggc gctttaatat
       61 ttttaatagc aggactatcc cccttcgtaa ttttaccgtt actgctggga taataaataa
      121 ttcatcttaa aagaaaccag aataataatt tatctcagga aaccaggata aaaggaaagt
      181 ttattctagg aatccttaaa aaatctttat tttcttttta tttcaaataa agctcccttc
      241 tgtcccatct taagtgatga gatatgggct gcgaaccttc cacattctgc aggatcggga
      301 gtttccagtt tttttactgc ataggcggcc atgaaggtgt cgcccagccc agtggggtca
      361 actatctctt ctggtggaaa agagggtata tcgtggatat ttttattttc ggctgttatt
      421 tgggaatata taacagcgcc acggtcgcct ctggttataa tcacctcttc cggcccaaaa
      481 ttggataata cctgggcgat ttctgcgcag ttatcactgg aatttcccag tatcacccgg
      541 gcttccagtt cgtctaaaaa caggaaatca accagtttga ggaatctttt atagtctctc
      601 cagggtttta aaaccacatt tccactttca acatgcctta aatatccctg ggcacccagg
      661 tagatgggta tatcttgttt acttagatat tgcactgttt cttggggaat gtcataggga
      721 gataatgggg ataagagtag ggcatcgtag gctttgaggt ttaaatcttt aaaattagcg
      781 ggttttattg gatttaccgg gatttgtgtt ttctgtattc tgtgattggg gttatcatca
      841 gggtagatat tctcaaattc catggttttc tcggtatata tggggattat ctgtgcggct
      901 cctgggaatt gtttcattaa gcctttgtcc atctttgaca tagtggtaat gacggtgttg
      961 tccattccca atcctgatag tactgctgat tggtagtaaa caccacctcc aacggtgtta
     1021 caaacgttgt ccctctttac aattcggtct ttggttaaag ggccgatgat catgaacttt
     1081 ggcatggtta aatctataaa caggatatca aataagttta gggatttttt gggttttgga
     1141 cctgctcatt ctactgtaga ccaactttac cggaatgcaa agcagtgcct attaagaatt
     1201 tattcacccc tcgatcattc aggttcttta cctcttctgg tgtgattccc ccgccgagta
     1261 tcaaagcatc ttccatccct ctgaaattat tcaggaggtc caagttaaat ccacttaaag
     1321 ttcccacccc agagatatca agtaaaataa tttcttccag tttcagtttt tccagtatag
     1381 atttaacttc accgaatgtg agagatggat ctctgcttag tatctgtcca tcttttacat
     1441 caatactaac catcatcctc tgcctgggga atttatggaa tatttcccct aaatctgccc
     1501 agcttgtaag ggtttccgtg gcgattatga ttttatctgc aaaatccagg gcaaaggcaa
     1561 agctctcaat atctttaacc ccccagtcca gcataaccgg cagcacatgg ttaattttcc
     1621 ggacgatttc caggttagaa cctctcccct caatggcatc caggtcagcc acgtagatac
     1681 gtttaaatcc ctctctttta agggatatgg ctatgtc
//
LOCUS       DAOQVW010000368         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_92986_length_1716_cov_2.888019, whole genome shotgun sequence.
ACCESSION   DAOQVW010000368 DAOQVW010000000
VERSION     DAOQVW010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92986_length_1716_cov_2.888019"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /gene="rtcA"
                     /locus_tag="PKK85_07540"
     CDS             complement(<1..748)
                     /gene="rtcA"
                     /locus_tag="PKK85_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HNS25907.1"
                     /translation="MIEIEGSYGEGGGALLRVATALAAFTGRSIHLDHIRANRPQTGL
                     MPQHLHAVKAIGQLSQAHMKGLEIGSEEIEFHPGKIEGQDITVDVKTAGSISLVLQAI
                     MIPAVKADSPFRVTVRGGTDVRWSPLMDYLKNVTLPLLGLMGYQAHLEIERRGYYPRG
                     GGQVEMMITPTPQLEPLTLTQLRVKRIRGVSHATRLPGHVARRQADGARKILGDEGYD
                     IDIRVEVNQDALGPGSGIVLWSEGQGRVGGS"
     gene            complement(779..>1716)
                     /locus_tag="PKK85_07545"
     CDS             complement(779..>1716)
                     /locus_tag="PKK85_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03576 family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNS25908.1"
                     /translation="LGGEKVLALNRTSSGILATILALVKPGQEVVHYLPERPSHPSIP
                     RSCQLIGASYRELAVGEELELREGTSLLVITGSTMDHQVLSQEDFKKAMGIAHQQEVP
                     VLVDDASGARLRTVIYGQPRATDMGADLVITSTDKLMEGPRGGLMSGKTTLIDFVKDK
                     AHQFGLEAQPPIMAAMVRALENYKPHHIKMAWERKDHLYTQLSRDCSGLEKTPTGVML
                     TPDAVMELVKAKTTLSPQDVSMLLAMLLLREENILTIPAVGMPGASATIRLDLASHDA
                     KRLTDGDITRSLHRALETLSGMVDDLDSCRRLLYQ"
BASE COUNT          337 a          489 c          464 g          426 t
ORIGIN      
        1 cacttccccc cacccgtccc tggccttcac tccataacac tattcccgag cctgggccca
       61 gggcatcctg gttgacctcc acccttatat ctatatcgta gccctcatca cctagtattt
      121 tccgggctcc atctgcctgt ctgcgggcca cgtggccagg caggcgggtg gcatgggaaa
      181 ctcccctgat acgtttcacc cggagctggg tgagggtgag gggctctagt tggggggtgg
      241 gggttatcat catttctacc tgtccccctc cccgggggta gtatcctctc ctttcgatct
      301 ccaagtgggc ctggtatccc atgaggccca agagtggtaa agtaacattt ttcaggtaat
      361 ccatcagggg ggaccagcgt acatcggttc cacccctgac agtgactctg aagggtgaat
      421 ctgccttcac agccgggatc atgattgcct gcagtaccag gctaatgctc cctgcagttt
      481 ttacatccac ggtgatgtcc tgtccttcta tctttccggg atggaactcg atttcttcgg
      541 agccaatttc cagtcccttc atatgggcct gggagagctg gccaatggct tttactgcgt
      601 ggaggtgctg gggcatgagg cctgtttggg ggcggttggc ccggatatgg tccaggtgta
      661 ttgatctgcc ggtgaatgca gccagggccg tggccaccct gagcagtgct cctccccctt
      721 ccccatagga tccttcaatt tctatcatag ctgtgttcac ctgtacaata acatgggact
      781 actggtagag cagtctacgg caggaatcca agtcatcaac cataccagaa agagtttcca
      841 gggcccggtg taagctacgc gttatatccc catcagtcag gcgcttggca tcatgggagg
      901 ccagatccag gcgtatagtg gctgatgctc ccggcatccc cacggcgggg atggttagaa
      961 tattttcctc ccttaaaagg agcatggcca gtaacataga cacatcctgg gggcttagtg
     1021 tggttttagc cttaacaagt tccatcacag cgtctggggt gagcatcacc ccggtgggcg
     1081 ttttctctag gccagagcag tcccgggata gctgagtgta gaggtggtcc ttcctttccc
     1141 aggccatctt aatatggtgg ggtttataat tctccagggc ccggaccata gcagccatga
     1201 tgggtggctg tgcttccaat ccaaactggt gggctttgtc ctttacaaag tcgattaaag
     1261 tagttttccc cgacatcaaa cccccccggg gtccttccat taacttgtca gtgctggtga
     1321 ttaccagatc cgcacccatg tcagtggctc ggggttgtcc atagattacg gtgcgcagcc
     1381 gggccccgga ggcatcatcc accaaaaccg gtacctcctg ctggtgggca atgcccatgg
     1441 cctttttaaa gtcctcctga cttagcacct ggtggtccat ggtggaacca gtgatgacca
     1501 gtaacgaggt gccttccctt aattccagtt cctcacccac agccagctcc cggtaggatg
     1561 cacctatcaa ctggcagctg cggggtatgg aggggtggga aggcctctca ggcagataat
     1621 gaaccacttc ctggccgggt ttcaccaatg ccagtatcgt agccagtata ccgctgctgg
     1681 tgcggttaag agccagtacc ttctcaccgc ccaggt
//
LOCUS       DAOQVW010000369         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_93234_length_1713_cov_3.628468, whole genome shotgun sequence.
ACCESSION   DAOQVW010000369 DAOQVW010000000
VERSION     DAOQVW010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93234_length_1713_cov_3.628468"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="PKK85_07550"
     CDS             <1..124
                     /locus_tag="PKK85_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2107 family protein"
                     /protein_id="HNS25909.1"
                     /translation="LIFLTYNHTLALLTFIAATAVITLILLRAIVRLEELGVDL"
     gene            121..615
                     /locus_tag="PKK85_07555"
     CDS             121..615
                     /locus_tag="PKK85_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EhaF family protein"
                     /protein_id="HNS25910.1"
                     /translation="MNIGRIWNSLANPRRIPRLFALILGLILLIGFFIPISLNDHQLY
                     PRPTPQSQINDQNPLAPYDRGGSPFEETGIVRAQYPQNSIELGQITAYLTPIAIGVKD
                     STYYFGTSILSSPGGLIDEILYYTRGFDTILESSILMMAFVIASWVALHFTMKRDEEG
                     DEKP"
     gene            612..1283
                     /locus_tag="PKK85_07560"
     CDS             612..1283
                     /locus_tag="PKK85_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EhaG family protein"
                     /protein_id="HNS25911.1"
                     /translation="MIVPVQVPELVVSLYPVAIWVGIITGLIGLLGISFQKNDLSVLI
                     LTDIVGVAMLIFVAGVATDLAEALILPGLVVELAEIMAISEILLSREIRKNNISGEDL
                     VPTALPLNMEILKTAPTFIAVVLIAYGVFLSGFTGGVIAGGGILFYILSKSARGLPVE
                     LFEGLAGASGIAWVFWIVGFLLFFVGPPFWLLGLMLAAFGILIKVASKVGLIGILTRE
                     EFKKE"
     gene            1290..>1713
                     /locus_tag="PKK85_07565"
     CDS             1290..>1713
                     /locus_tag="PKK85_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25912.1"
                     /translation="MDLATIGGQLLGTIPLGDIVLYLTPFNLFMFGGTLAFTTLIAIS
                     RTETQVEAEFGTLKDKEVKVNLEEFKIRRFLAIVCGLATAGAMITGDLFNFTLFVTLI
                     GIVNIGIVSAVRQVDVLNAAFQYGLIAMMASLPLFGGAA"
BASE COUNT          438 a          404 c          405 g          466 t
ORIGIN      
        1 actaatattt ttaacctata accacaccct agcccttcta accttcattg ctgctacagc
       61 cgtcattact ctaatactgt taagggccat tgtacgtctg gaagaattgg gggtggactt
      121 gtgaatattg gaagaatatg gaattccctg gccaacccta gacgtatacc acgattattc
      181 gcattaatac tgggtctgat cctattaatc ggctttttta tcccaataag cctaaatgac
      241 caccaattat accctcgacc cactccccag agccagatca acgaccaaaa cccattagcc
      301 ccatatgacc gaggaggatc accctttgag gaaacaggga ttgttagggc ccagtatccc
      361 cagaattcaa ttgaactagg ccagataaca gcatacctta cgcccatagc tataggggtt
      421 aaagacagca cctattactt tggtacgtca atcctctcct cacctggtgg cctcatcgat
      481 gagattctct attacacgcg tggatttgac accatactgg agtcctccat tctaatgatg
      541 gccttcgtca ttgccagttg ggtggcactg cacttcacca tgaaaagaga tgaagaagga
      601 gatgaaaaac catgatagtc cctgtacaag tgcctgaact ggtggtatca ctctatccag
      661 tggctatctg ggtggggata ataactggac taattggact tctgggcata tcattccaga
      721 agaacgatct atcagtgctg atactaactg atatagtggg tgtggccatg ctaatcttcg
      781 tggcaggagt agccactgac ctggccgagg cccttatcct gccgggatta gtggtggaat
      841 tagctgaaat aatggccatt tctgaaattc tcctcagccg agagattaga aagaacaata
      901 taagtggaga agacttagtc cccactgcat tacccttgaa catggagata cttaaaacag
      961 ctcccacttt cattgcggtt gtacttattg cctacggcgt attcctcagc ggttttacag
     1021 gaggagttat agctggtgga ggaatattgt tctatatact ttccaagagt gcccggggct
     1081 tgcctgtaga attgtttgag ggattggctg gtgcgtcagg tattgcctgg gtcttctgga
     1141 tagtgggatt cctgctattc ttcgttggcc caccattctg gttactgggg ttgatgttag
     1201 cagcctttgg tatacttatt aaggtggcct ctaaggtggg gctcattgga atattgactc
     1261 gtgaagaatt caagaaggag tagaaaaaca tggatttagc cactatcgga ggacaactcc
     1321 taggaaccat ccccctgggg gatattgtac tgtacctgac ccccttcaac ctcttcatgt
     1381 ttggaggaac cttggccttc accactctca ttgccatcag ccgtaccgaa acccaggttg
     1441 aagcagagtt cgggaccctg aaggataagg aagttaaggt gaacctggaa gagttcaaaa
     1501 ttagacgttt cctggccatt gtatgtggac tggcaactgc cggggccatg atcaccggag
     1561 acttgttcaa cttcaccctc ttcgtgaccc tcataggcat agttaacatc ggcatcgtgt
     1621 ctgccgtgag acaggtagat gtcctgaatg cggccttcca gtacggctta atagctatga
     1681 tggcgtcact accccttttc ggtggagctg ctg
//
LOCUS       DAOQVW010000370         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_93377_length_1712_cov_2.296319, whole genome shotgun sequence.
ACCESSION   DAOQVW010000370 DAOQVW010000000
VERSION     DAOQVW010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93377_length_1712_cov_2.296319"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PKK85_07570"
     CDS             <1..438
                     /locus_tag="PKK85_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25913.1"
                     /translation="MMYKRENLEGIRYNEDIRNLNDHKFNLDLSYQYEYYFIPEVLSK
                     YRLHGRNTIFSDMEGWYKDYMAIGKFIAREYGDRLSSRQNKKKLFHLIFTTPFYSAVK
                     QDPWNKLNLIYGLKLPPYAIYTLIKLYFQNHNGQNEVYKSTSK"
     gene            complement(462..>1712)
                     /gene="rfbH"
                     /locus_tag="PKK85_07575"
     CDS             complement(462..>1712)
                     /gene="rfbH"
                     /locus_tag="PKK85_07575"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNS25914.1"
                     /translation="RKSKETFNPGQDSVNYAGRVYDEKEMISLVDSALDFWLTAGRYA
                     DSLEKELANFLGVKHCLLTNSGSSANLLAISALTSPKLGDRRLKPGDEVITTACGFPT
                     TLNPIIQNQLKPVFVDVELGNYNINADLIEGALSKKTKAICIPHTLGNPFNIEKISEI
                     SKKHDLWFIEDNCDALGSKFKNRYTGSFGDLSTLSFYPAHHITMGEGGAVLTNDPLLK
                     EIVASFRDWGRDCWCDPGHDDTCGRRFNWQLGSLPYGYDHKYIYSHIGYNLKVTDMQA
                     AVGLRQLEKLPKFIQDRIKNFEILKDLFLEHENYLILPQVEKGAEPSWFGFPILVRED
                     APFNRDDIVNFLENNKIATRMLFGGNLIRQPAYEDVNFRVAGSLENTDLVMNNLFWIG
                     VYPGIAEQKMNYVISMVKKFFSGL"
BASE COUNT          548 a          338 c          293 g          533 t
ORIGIN      
        1 atgatgtaca aaagagaaaa tttggaagga atcagatata atgaagatat taggaaccta
       61 aatgatcaca aatttaacct agatttatca tatcagtatg aatactattt tataccagaa
      121 gtactctcaa aatacaggtt acatggccga aacactattt tttcggatat ggaagggtgg
      181 tataaagatt atatggctat tggtaagttc attgccagag aatacggtga tagacttagt
      241 tctcgacaaa ataaaaagaa actgttccac ttgatcttta ctacaccctt ttatagcgcc
      301 gtaaaacaag acccctggaa caagttaaat ctgatttatg ggctaaaact tcctccttac
      361 gctatttaca cacttattaa actatatttt cagaatcata atggccagaa tgaagtatat
      421 aaaagcactt caaaatgaat tcttaataac cattaagggg attataatcc tgaaaaaaac
      481 tttttaacca tggaaatcac gtaattcatt ttttgttctg ctattccggg atatacacca
      541 atccagaata ggttattcat caccaaatca gtgttttcta aggaaccagc caccctgaaa
      601 tttacatctt cataagctgg ttgccggatt aaatttcctc caaatagcat tctggttgct
      661 attttattat tttccagaaa attaacaata tcatcacgat tgaaaggtgc atcttccctc
      721 accagaatag ggaaaccaaa ccaactgggt tctgcccctt tttctacttg gggtaggatc
      781 aaataattct catgttccaa aaataggtct ttaaggattt caaaattctt gattctatct
      841 tgaataaact ttggtaactt ttccagttgt cttaaaccaa ctgcagcttg catatcggtg
      901 actttaaggt tatacccgat gtgagaatag atatatttat gatcatatcc atagggcaga
      961 gaacccaatt gccagttaaa tctgcggcca caggtatcat catggccagg gtcacaccaa
     1021 caatctcttc cccaatccct aaaagaggcc acaatctctt taagtagggg gtcgttggtt
     1081 aaaactgctc ctccctctcc catggttatg tggtgagctg gataaaagct gagggtagac
     1141 aaatccccaa agctaccagt atacctattt ttgaatttag aacccagagc atcacagtta
     1201 tcctctataa accaaagatc atgtttttta gagatttcag atattttttc aatattaaac
     1261 gggtttccca aagtgtgtgg tatgcatatc gctttagtct tctttgataa ggctccttca
     1321 atgagatccg catttatgtt gtagtttcca agttccacgt caacaaatac tggttttaac
     1381 tggttttgta ttatgggatt caaagtggtg ggaaatccac aagcagtagt gataacttca
     1441 tctccaggtt tcaatctcct gtcacccaat ttaggggaag ttaaagctga aattgccagt
     1501 aaattagctg atgacccgga gttggtgaga agacaatgtt taacacctaa aaaattggca
     1561 agttccttct ctaaactatc agcatatctg ccagccgtta accagaaatc aagggcagaa
     1621 tcaaccagac taatcatttc tttttcatca taaactcttc cagcatagtt aacgctgtcc
     1681 tggccaggat tgaatgtttc ctttgatttc ct
//
LOCUS       DAOQVW010000371         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_93697_length_1708_cov_2.774350, whole genome shotgun sequence.
ACCESSION   DAOQVW010000371 DAOQVW010000000
VERSION     DAOQVW010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93697_length_1708_cov_2.774350"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1372)
                     /locus_tag="PKK85_07580"
     CDS             complement(131..1372)
                     /locus_tag="PKK85_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNS25915.1"
                     /translation="MYNNKKLIMGFLAISLFTIPISLAPVVALDWPMFHLNEQRTGKL
                     EQPADFTPASWYFQAGDSIKSSPAIMDKVIYFGSSDGYVYAVNLELGNRLWRYETEGA
                     VTSSPVLLNETLYVGSMDGYLYAQDTKNGEVEWKYKTGNSIESSPTIDGDTIYVGSDD
                     GRLYAISTNGSLLWEFLSGNAIKSSPIVNGSTVYFGSDDGKVYALNAETGEKKWEYTT
                     GDKVQSSPAILNETLYVGSNDGKIYALNINTGVLDWNYEMGKPVKSSPTIDSADDSLF
                     IGADNGKVVCVDTRNGTEKWVVEGLGAVKSTAALFDNKIVFGSDDGTVYILNKYTGKE
                     EWTYNPGYYVMNAPFQSSPVTYGNMIYIGGNDGYLYALKSDKESGPISIFAYYIGGAL
                     VVILVLLVGLRSIRGRGKKGE"
     gene            complement(1399..>1708)
                     /locus_tag="PKK85_07585"
     CDS             complement(1399..>1708)
                     /locus_tag="PKK85_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS25916.1"
                     /translation="CFVGFGIMISARVGTQEDYIQMVMPFSMPMMFISGVFYPLETMP
                     WIFQKIAYLAPLTYANIALREVMLQGAGIGDIWVEVAVLLGFTLLFFAMGVTRFNRDI
                     "
BASE COUNT          507 a          385 c          302 g          514 t
ORIGIN      
        1 aaggaatagt cagtgaaatc ttgaaacctc aatggtttca tattattttt tatttttaaa
       61 ccattttaca tagatgcttc aataaaaaat taattaaagc cgtataaaac ggcgattttt
      121 caaaaaattt ctattcgcct ttttttcccc ggccccgtat ggacctcaga cccactagta
      181 agactaagat cactactaag gctcctccga tgtagtaggc aaatatggat atgggtccgc
      241 tttccttgtc gctttttagg gcatagaggt atccgtcatt tcctccaatg tagatcatgt
      301 taccataagt aacaggcgat gactggaatg gagcgttcat tacgtagtac ccggggttgt
      361 aagtccactc ttctttcccg gtgtacttgt ttagaatgta aaccgttcca tcatcagatc
      421 caaagacaat tttattatca aagagcgctg ctgtagattt aactgctccc aaaccttcaa
      481 ccacccattt ttcagttcca tttctggtgt ctacacagac caccttgcca ttatccgctc
      541 ctatgaaaag gctgtcatcg gccgaatcga tagttggaga agatttaaca ggtttaccca
      601 tttcataatt ccaatcgaga accccagtat taatatttaa ggcgtatatt ttgccgtcat
      661 tggatcccac atataatgtt tcgtttaaaa tggctggtga agactgtact ttgtctccag
      721 tagtgtattc ccattttttc tcacccgttt ctgcattcag ggcgtatacc ttaccatcgt
      781 ctgatccgaa gtaaacagta ctcccattca ctatagggga ggatttaatg gcatttccac
      841 ttaaaaattc ccacaataaa gatccattag tcgaaattgc gtataaacgg ccatcatcgg
      901 atcccacgta aatagtatca ccatcaatgg taggggaaga ttctatggaa ttaccggttt
      961 tatatttcca ctccacttca ccatttttag tgtcctgagc gtaaagatac ccgtccatgg
     1021 aaccaacgta taaagtttcg tttaatagca ctggagagga agtgacagct ccctcagttt
     1081 catatctcca taagcgattc cccaactcaa gattcactgc ataaacatac ccatcacttg
     1141 atccaaaata tattaccttg tccattatgg ctggggaaga tttgattgaa tctccggctt
     1201 ggaaatacca ggatgctggt gtaaagtcag ctggctgttc taatttccca gttcgttgtt
     1261 cattcagatg aaacataggc cagtcaagag ctaccactgg ggctaatgaa ataggtatgg
     1321 taaataaact aatagccaga aatcccataa ttaacttttt gttgttatac ataatatcac
     1381 ctttaatctc attatcttct aaatatcccg attaaatctg gtaacaccca tagcaaagaa
     1441 aagcagggtg aaacctaaga gaactgctac ttcgacccat atatctccaa ttccggcccc
     1501 ttgcaacata acctccctta atgcaatgtt tgcataggtt aaaggtgcta aataagctat
     1561 tttttggaaa atccagggca tagtttccag gggatagaac actccagata tgaacatcat
     1621 gggcatgcta aagggcatca ccatctggat gtagtcttcc tgtgtaccta ccctagcaga
     1681 aatcatgatc ccaaagccta caaaacac
//
LOCUS       DAOQVW010000372         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_93947_length_1706_cov_1.701393, whole genome shotgun sequence.
ACCESSION   DAOQVW010000372 DAOQVW010000000
VERSION     DAOQVW010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93947_length_1706_cov_1.701393"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PKK85_07590"
     CDS             complement(<1..1071)
                     /locus_tag="PKK85_07590"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNS25917.1"
                     /translation="MHPIEAREGLKTKNGDGLFVPYDELMVAAKQTQFCERLRVIHPV
                     IFLMTLLTYRSFHLVPTFRELWQHYVRLTGYEGYNNSEVIKYQSFYVRFDEYLVNYLK
                     MVVSLAMEKLESNSPWKLRGSLEQFSTVYILDNTLVRLHDKLNLLFPAARTRKPGKSA
                     GFKVSVLFNAVAHGPCSISVFPERTHDIKTFKIGKWVENSLIILDLGFFKTWNFDKIV
                     RYGGSFLIRLKSNSKPIVTKILLPDADSRYKDLIGLPVHEVLGKLPHGPVAMEVRVSF
                     RRRKYRNKRGRIDFCDFYCYAEFNHTAQRWHTYLTNLSPEEFTVPEICALYAYRWTIE
                     LLFKEIKSDNELGQKKSVNAYLS"
     gene            complement(1479..>1706)
                     /locus_tag="PKK85_07595"
     CDS             complement(1479..>1706)
                     /locus_tag="PKK85_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25918.1"
                     /translation="YLFAAAQQAGSSSSVVIWGPKVEGKSGHANVLINGKLFDSTSDI
                     TDVSYFKNHYELVGVADTYEMIAFYLDPSVW"
BASE COUNT          540 a          344 c          303 g          519 t
ORIGIN      
        1 actcaaatat gcgttaacag actttttctg gcctaattca ttatcacttt ttatctcttt
       61 aaataatagc tcaatggtcc atctataggc atataatgcg cagatttccg ggacagtaaa
      121 ttcctctgga gataaatttg ttaagtaggt atgccatctc tgggcagtat gattaaattc
      181 agcataacaa taaaagtcac agaaatcaat tctacctcgt ttatttcgat attttcttct
      241 ccgaaatgag acccgcactt ccatagcaac aggcccatgt ggaagtttcc cgagtacctc
      301 atgaacgggc agaccaatga gatctttata acgagaatct gcgtcaggaa ggagaatttt
      361 agtgacaatt ggctttgaat tcgacttcaa ccggattaga aaactaccac catacctcac
      421 aattttatcg aagttccacg tcttgaaaaa acctagatct aagattatca aggagttttc
      481 aacccatttt ccaattttaa acgttttaat gtcgtgggtt ctttctggaa acacagaaat
      541 gctgcaagga ccatgtgcta ctgcattgaa tagaacagat accttaaacc cagctgactt
      601 tcccggtttc ctggttctgg ctgcaggaaa taggagattt aacttgtcat ggagccgaac
      661 caaggtatta tccaagatat ataccgtact aaactgctcc aaagaacctc gtaatttcca
      721 tggagaattg gactctaatt tctccattgc taaagaaact accattttaa gataattcac
      781 taagtattca tcaaatctta cataaaaact ctgatatttg atgacttcag aattattata
      841 gccctcatag ccagttaatc gcacataatg ttgccataac tcgcgaaatg ttggtaccaa
      901 atgaaaactt cgatacgtga gtaaagtcat gagaaatata accgggtgga ttaccctcag
      961 cctctcacaa aattgggtct gcttggcggc aaccattaac tcatcataag ggacgaataa
     1021 tccatccccg ttcttagttt tgagcccttc ccgggcttcg ataggatgca cgaccacttc
     1081 gttgctaaac tatataaagc taactcaatc tctgatcgcg aaaaaatatg aaaattgagg
     1141 tgaaaagtat gacaagagtc ggttgcttaa aagggcgtga tccaaaactg agatagatga
     1201 attttattct aaaattttat taatatggaa atacttgaga tattgatttg ggggtgtatt
     1261 gtcgttataa tctatggaat atttacatct caatacggcc ttttcttatg ggggttggga
     1321 ggattactgc tctttgcggc attaaatggt gagttataaa ttactgaaaa catatgtgac
     1381 ggtatatctt ataccatgaa ttagtccctt ttgtcaattt agattaaggt ctaaatgagt
     1441 atatttttaa aatccatata aatacgaagg accattcatt accacaccga tggatccagg
     1501 taaaatgcaa tcatctcata tgtatctgct acaccaacca gttcatagtg atttttgaag
     1561 tacgatacat cagtaatatc actcgtacta tcgaacaatt tcccattaat cagcacattt
     1621 gcatgacctg attttccttc cacctttggt ccccaaatga ccaccgatga agaactacct
     1681 gcctgctgtg cagcggcaaa taggta
//
LOCUS       DAOQVW010000373         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_94089_length_1704_cov_2.794421, whole genome shotgun sequence.
ACCESSION   DAOQVW010000373 DAOQVW010000000
VERSION     DAOQVW010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94089_length_1704_cov_2.794421"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKK85_07600"
     CDS             complement(<1..375)
                     /locus_tag="PKK85_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNS25919.1"
                     /translation="MLQVAIIGASGYTGGELLRFMHNHPQVEVVAATSRQHAGQYISK
                     VHPHLQDMDLKFQDISPGDLDADLVFTATPHGASMNIVPPLVEKGMKVVDLSGDYRFK
                     DLETYQKWYGLKHTHPLDAVYGM"
     gene            913..1203
                     /locus_tag="PKK85_07605"
     CDS             913..1203
                     /locus_tag="PKK85_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25920.1"
                     /translation="MKRFICHGWMIIICPMIGSPIPVWRPGHGGGVFVGECTILLLLT
                     VFVPHHLRHAYFFYQYPLMAWSSVQFKNVLTPIQGATGGLIIFKGILEGLFV"
     gene            complement(1162..>1704)
                     /locus_tag="PKK85_07610"
     CDS             complement(1162..>1704)
                     /locus_tag="PKK85_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 family protein"
                     /protein_id="HNS25921.1"
                     /translation="YAFADEVDIPGEELARIEGADLVITYILHPDLSLDLVNLIHQKV
                     DWIIVGAWRGEGFKKQLTSYGNVTCPENMCDLIENGNPTYDEFVARFGRPVVRINCQG
                     DRVVDIQVLRCSPCGSTHYLAQELKGESTINLPIKAGLKIQHYPCRAPRMRLFSDDEC
                     KKEIAANLHKEAFQDALEDD"
BASE COUNT          408 a          410 c          423 g          463 t
ORIGIN      
        1 catcccatac acagcatcca gggggtgggt gtgctttaaa ccataccact tctggtaggt
       61 ttccaggtcc ttgaagcggt agtctccact tagatccacc accttcatgc ccttctccac
      121 cagtgggggt acgatgttca tggaggcccc gtggggggtg gctgtgaaga ccagatcagc
      181 atccaaatct ccgggtgaga tatcctggaa tttcaaatcc atatcctgga gatggggatg
      241 taccttgctg atgtactgtc ctgcgtgctg ccgggatgtg gccgccacca cctccacctg
      301 gggatggttg tgcataaatc ttaagagttc tccaccagtg tagccactgg cccctattat
      361 agctacttgt aacatgatgc tcaccaaatt gatccttagg gattaacttc actatgatta
      421 tatcacctag taggggataa gggttgccct taccgattcc cttaacagac tggtttatgg
      481 gctgaccatg gaattagggg ggatttccct ggcatagcac ccctctacta ggagggatgg
      541 tgaaataggt gtaagcacat gctggtggag aattattaac aacctcccac tagttcttaa
      601 caaattataa atccagttat aggtccaata gggcatagat atttcctgta aagacccggt
      661 atccttaaaa gaccataccc tggtaggtgt atgattagtg gagcagggag tatcttaaga
      721 accatttcca gagtgctggt tcgggtagtt agagattaac tgaattcctt ttgataaaac
      781 catcattaaa tggtaggata cggagttgat aggactttaa aagcagttca tcctaagctg
      841 tcatataaaa acccttaaaa tactcggtaa acgtcgaaaa gggcatagaa cacccggaaa
      901 tacggggatt aaatgaaaag atttatatgt catggctgga tgatcatcat ttgtccaatg
      961 ataggctctc caatccctgt ttggaggcct ggacacggag gcggtgtttt cgtcggtgag
     1021 tgcacaatat tgttattgtt aacggttttt gtgcctcacc atctacgcca tgcttacttt
     1081 ttctaccagt accccctgat ggcctggtca tcagtccagt ttaaaaatgt tctaacaccc
     1141 attcaaggtg ctactggggg gttaatcatc ttcaagggca tcctggaagg cctctttgtg
     1201 taagttggcg gctatctcct ttttacactc gtcatcagaa aatagtcgca tcctgggggc
     1261 gcggcagggg tagtgctgga tcttgagccc ggccttgatg gggaggttaa tggtgctttc
     1321 ccccttcaac tcctgggcta ggtagtgggt ggagccacag ggtgagcatc gcagcacctg
     1381 gatatccacc acccgatcac cctgacagtt gatcctgacc accgggcgtc cgaatcttgc
     1441 cacgaactcg tcataggtgg ggtttccgtt ttctatgagg tcacacatgt tctctgggca
     1501 ggtgacattg ccgtagctgg ttaactgctt tttaaatccc tcacccctcc atgcacccac
     1561 tatgatccag tccacctttt ggtggatgag gttcaccagg tccagggaga gatccgggtg
     1621 caggatgtag gtgatgacca ggtctgcccc ctctatcctg gccagttcct cccctgggat
     1681 atccacctca tcagcgaagg cata
//
LOCUS       DAOQVW010000374         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_94744_length_1697_cov_3.338611, whole genome shotgun sequence.
ACCESSION   DAOQVW010000374 DAOQVW010000000
VERSION     DAOQVW010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94744_length_1697_cov_3.338611"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKK85_07615"
     CDS             <1..665
                     /locus_tag="PKK85_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNS25922.1"
                     /translation="VEEINRETEVQAFAVVGSHPAELSRLVREGTTLEEGEELMRGAL
                     ETAQEMVLEGQAVAIGEVGRPHYEITPKEMEIHNRLLLYAMELAKEADCPVQLHTESA
                     GPDEFLEFAQLADQAGLKREKVIKHFSGPLVLQEENHGLTPSLISSGDVIQAGLKKGT
                     NFLMETDYLDDLSRPGAVLGPKTVPRRTRKLFNAGLISEDDVHRIHVENVERVYGIEL
                     VF"
     gene            complement(700..1278)
                     /locus_tag="PKK85_07620"
     CDS             complement(700..1278)
                     /locus_tag="PKK85_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00267 family protein"
                     /protein_id="HNS25923.1"
                     /translation="MDIREFITEYFKMSRYVALGTMDGILAVMGVILAASGVAGIAGS
                     DIPNYVIGLTGLSGGLALAMSNAFGSFIGERAEEVRTIRELEQKMMLEEGKLDDTHIH
                     QKAKKRIFMSMVTHGFSSFTGSFVPVLPFLLLSNRFSATIWTIVLCFMALVLLGIYLG
                     RVARESLLKTSVEIIIIGVVISVISFLIGGSH"
     gene            complement(1316..>1697)
                     /locus_tag="PKK85_07625"
     CDS             complement(1316..>1697)
                     /locus_tag="PKK85_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HNS25924.1"
                     /translation="LVGSAISGSMGFNAHYANMIGALFLATGQDEAHVVEGSLGITLA
                     QEDGGDLYFSVTLPDVPLATYGGGTRLETARECLKIMDAYGSGKVDKFAEIVAGIVLA
                     GELSLLGALGAGHLARAHRELGRG"
BASE COUNT          435 a          454 c          433 g          375 t
ORIGIN      
        1 acgtggagga aataaaccgg gaaaccgagg tccaagcctt tgcagtggtg ggctcccatc
       61 ctgcggagct cagccgccta gtacgagaag gaaccaccct ggaggagggt gaagagttga
      121 tgcggggggc cctggaaacc gcccaagaaa tggtgctgga gggccaggcc gtggccatag
      181 gcgaagtggg acgcccccac tacgagatca cccccaaaga gatggagatc cacaacaggc
      241 tgctccttta tgccatggaa ctggccaagg aggctgattg tccagtgcag cttcacaccg
      301 aaagtgcggg gcccgatgag tttctggaat ttgcacaact agccgaccag gcgggtttga
      361 aacgggaaaa agtcatcaaa cacttctccg gacccctagt gttgcaggaa gaaaaccatg
      421 gactaacacc gtccttgata tccagtggag atgtgatcca ggcgggactt aaaaaaggaa
      481 ctaattttct catggaaacc gattacctgg atgatttatc ccggccgggg gcagttttag
      541 ggccgaaaac cgtgccaaga agaactcgca aactctttaa cgccggctta ataagtgaag
      601 atgatgtaca ccgcatccat gtggagaacg tggagagggt gtacgggata gaacttgtat
      661 tttaggtaga atcagagatc accaccatat tctttttttt cagtggctgc caccgatgag
      721 gaaactgatc acgctgatta caactccaat gatgatgatt tctacactgg ttttcagtag
      781 actctcccgg gccaccctgc ccaggtagat ccccagaagc accagcgcca taaagcagag
      841 gactatagtc catatcgttg ctgaaaatcg gtttgaaaga agtagaaatg gcaacaccgg
      901 cacaaaggaa ccagtgaagc tggaaaagcc gtgggtgacc atgctcatga agatgcgctt
      961 tttggccttc tggtggatgt gggtgtcatc cagcttaccc tcctccagca tcatcttctg
     1021 ctccagttcc ctgatggtcc tcacttcttc ggcccgttct ccaatgaatg atccgaaggc
     1081 attggacatg gccagggcaa gacccccact taaaccagtt aatccaatga catagttggg
     1141 tatatcggag ccagcaatcc cagccacccc actggcagcc aggattactc ccataacagc
     1201 tagaattccg tccatagttc ccagggccac gtaacgactc attttaaagt attcggttat
     1261 gaattcccgt atatccattg tagatgttct ccccttgtaa gaaaaaaata gctccctatc
     1321 ctctgcccag ttcccggtga gcacgggcca ggtgacctgc acctagtgct cctaagagtg
     1381 agagctcccc ggccagtacg attcctgcca ctatttctgc gaacttatct accttacccg
     1441 aaccgtaggc gtccatgatc tttaaacatt cccgggcggt ttccagacgg gttcctcctc
     1501 cgtaggttgc caggggcacg tcgggtaggg ttacagagaa gtacaggtct cccccatctt
     1561 cctgggccag ggttattccc agactaccct ccactacatg ggcttcatcc tgtccagtgg
     1621 caaggaacag ggcaccgatc atgttggcat agtgggcgtt aaagcccata ctacctgata
     1681 tggcagatcc caccagg
//
LOCUS       DAOQVW010000375         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_94807_length_1697_cov_1.451888, whole genome shotgun sequence.
ACCESSION   DAOQVW010000375 DAOQVW010000000
VERSION     DAOQVW010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94807_length_1697_cov_1.451888"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKK85_07630"
     CDS             complement(<1..321)
                     /locus_tag="PKK85_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNS25925.1"
                     /translation="MIDAHCHVDFKEYNKNRGEVMQRAQEKLRAIINSGASLGGNRRT
                     LKLQEEYPGFLYATLGFHPHNAVKADSTIIDQALGEIVDNIDKTVALGETGLDFHQIK
                     GEEQK"
     gene            complement(510..1277)
                     /gene="uppS"
                     /locus_tag="PKK85_07635"
     CDS             complement(510..1277)
                     /gene="uppS"
                     /locus_tag="PKK85_07635"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNS25926.1"
                     /translation="MSLLKPLYTVYEWYISRNLRTENMPRHVALIMDGNRRYSKIQGD
                     MDSIEGHKRGVYTLEKVLDWCIDLGIEIVTVYAFSIENFKRPPEEVKGLMELFKYNFA
                     SIAQNKRIHKNEVKLKAVGKLELLPDDVREAIRHAEESTSKYNKRQVNIAIGYDGRLE
                     IVDAMQKIADQVKDGKLDPEDIDEKVINENLYTAGLADPNLIIRTSGEERLSGFLLWQ
                     SSYSELYFCDSLWPQLRKVDFLRALRDYQQRERRFGV"
     gene            complement(1434..>1697)
                     /locus_tag="PKK85_07640"
     CDS             complement(1434..>1697)
                     /locus_tag="PKK85_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNS25927.1"
                     /translation="KHHHILIEEAIHDNSNFILAPDGITGNLIFRSLVLSGCGESHGA
                     VTLGMDVILIDTSRSLTVEGYTRALKFAKYLMDSKINNLRNKI"
BASE COUNT          442 a          354 c          284 g          617 t
ORIGIN      
        1 tttttgttct tcacccttta tctggtggaa atcaagacct gtctcgccta aggccactgt
       61 tttatctata ttatcaacaa tttcccctag ggcctggtct ataatcgtag aatcggcttt
      121 aactgcattg tgtgggtgga aaccaagagt ggcatataga aatccaggat attcttcttg
      181 cagttttaat gtgcgccggt taccacctag acttgctccg gagttgatga tggcccgtag
      241 tttctcctgg gccctttgca ttacttctcc cctgtttttg ttgtattctt tgaagtctac
      301 atggcagtgt gcgtcgatca ttttccaaac tcccgattat atgatatgaa aattatgtat
      361 taaatcttaa cctccagtct gaatgaaaat aaaaaaaatc ctattcctat ctgaatttta
      421 aaccccccta atgaaaataa aagaaattct atctgtatct aataattaac cccaccttat
      481 gaaaataaaa aatttacaac tcctaattat tataccccaa accttctttc cctctgttgg
      541 taatctctta aagcccttag gaaatctact ttcctgagtt gtggccataa actgtcacag
      601 aaatagagtt cactgtagga tgattgccag agtaagaatc cgctgagtct ttcttctcca
      661 ctggttctta tgatcaggtt ggggtccgcc agtccggcgg tgtatagatt ttcatttata
      721 accttttcat ctatatcctc tgggtcgagt ttaccatctt taacctgatc tgctattttc
      781 tgcatggcat cgactatttc taaacgtccg tcgtatccta tagcgatatt tacctgtctt
      841 ttattgtatt tgctggtgga ttcttccgca tgtcttatgg cttctcttac atcatccggt
      901 aagagttcca atttacccac cgcctttagc tttacttcgt ttttatggat ccttttattt
      961 tgggctatgg atgcgaagtt gtacttgaat agttccatta aaccttttac ttcctctggt
     1021 ggtcttttaa agttttctat ggaaaatgcg tatacggtga ctatctctat ccctaaatca
     1081 atacaccagt ccagcacctt ttccagggtg tacactcccc gtttgtgacc ttcaattgaa
     1141 tccatgtcac cctgtatttt ggagtatctt ctgttaccgt ccattattag ggctacgtgt
     1201 ctgggcatgt tttctgtacg gaggtttcga gagatgtacc attcgtatac tgtatatagt
     1261 ggttttaaaa gtgacattgt tactcccagc atcttagtat atttagattt ataagtgatc
     1321 ctataaaaaa taaactatca taatcagata taaaaaagat gcttattgac ctattattta
     1381 cttaaatcag tgcttttttt ctttttaatc ttattttcca tttcaaataa ttgctaaatt
     1441 ttatttctaa ggttattaat ctttgaatcc atcaggtatt tggcgaattt caacgcccgg
     1501 gtataacctt ccactgtgag ggatcgtgaa gtatcgatta atatcacgtc cattcccagg
     1561 gttacagcgc catggctttc tccacatcct gataaaacaa gagatctgaa tattaaattc
     1621 ccggtgatac catctggtgc cagtataaaa tttgaattat catgaattgc ttcttcaatc
     1681 aatatatgat gatgttt
//
LOCUS       DAOQVW010000376         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_94911_length_1695_cov_4.232927, whole genome shotgun sequence.
ACCESSION   DAOQVW010000376 DAOQVW010000000
VERSION     DAOQVW010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94911_length_1695_cov_4.232927"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKK85_07645"
     CDS             complement(<1..486)
                     /locus_tag="PKK85_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HNS25928.1"
                     /translation="MKKHGIIVLVAVMAVLLCGAVSAADSSTAEVEGLSEVNAPAEAV
                     DPILWVTVNYEYASDDINPDIEVKDSHNAIVQYDKSPYSRNLYKLNFTYPGVTNGTLF
                     KVTVNAPGYTTQTQNVAVNQAGSDPEFYGNAVFNMEATSNYQLGREVTKKADQLLNFA
                     TA"
BASE COUNT          516 a          334 c          293 g          552 t
ORIGIN      
        1 ggctgtggcg aagttcaaga gctggtcagc tttcttggtg acttctcggc ccagctggta
       61 gttgctagtg gcttccatgt tgaagactgc atttccataa aactctggat cagaaccagc
      121 ctggtttact gccacgttct gggtctgagt agtgtaacca ggggcgttaa cagttacctt
      181 aaagagtgtg ccgttggtta caccaggata agtaaagttc aacttgtaca ggttgcggga
      241 atagggtgac ttatcatact gtacaatggc attatgagag tctttgactt caatgtccgg
      301 attaatgtca tccgaagcat actcgtagtt aactgtcacc cataaaatgg gatccactgc
      361 ctcagctgga gcattaactt cactgagtcc ttccacctcc gcggtagatg aatctgcagc
      421 tgacactgct ccacataaca aaacagccat gactgctaca agaactatta ttccatgttt
      481 tttcatcttt tcacctcctt caattttgtt tgacttatta aaagtcaacc ccactgtgtt
      541 gtaatactgt atataatatt tgtgttatga ctggtttagc gattcgcggc agaaatttaa
      601 tacaaactat gaaattaata caatagggga atttttacct taaaaagtaa tactctagta
      661 ggtttatata ggaaaattta atcttaaaaa ttcaatttct acttggattt gtccttcacc
      721 actacgtgca cgtaaaatcc taccatttat gcccagttat aagctaaaaa tagcaaattg
      781 cagccatcaa aattaaaaaa atccggacta aattgaattt acgagttatt acctccaagc
      841 caaaggagtg accaactgga tcataatcat ctttttcctg accctgtatc ttttaatgtt
      901 ttccaccgaa caggtgaaac cataatttac acctttattc tttgctgttg ccacagtggt
      961 caatccttta ctatctgttc cctcacccct gagagttact gttatctttt cccgatgctc
     1021 cggtgactat tcatattaaa cccccccaga gtggcattgt agcggatttc ctttactggg
     1081 gtgattgttt atctccatct tataatgtgg ggccttattg gtttcctaat tgatcctgta
     1141 ttgaaacttc cttgaataaa aaaaagtatc taactggatt attctataag atttttttgt
     1201 tgaaagtatc ttcaatctca aaaaaaatct acattttaaa ataagacatt aatgtggaag
     1261 tagggatcaa tggctaagac ccccattcca gtaaggaggg ttatatttag ataaatgtga
     1321 acagggcatt tcaatacgtt tgagcaaaat tttttgatgc cctggttcaa tataagggac
     1381 atatggctaa ataataagcc atacctcccg ggatttgcca tggtgtgaca cagtaagtag
     1441 attgcatcta tcattataaa tactttttta ttagtaagta ttaccaattt cctaaaaata
     1501 gttcgatttt tattacaaca aaaattaaca attgatagct ctatacaatt ataaaaagtt
     1561 taatcatcca tgaatctatc aataatcagg atagcatgtt caactgctcc atctgcaagt
     1621 ttttcaggat taacagttga tagatcatct aaaaatagtt ccacagtcac atctacatcc
     1681 aatgcatcgt tgaaa
//
LOCUS       DAOQVW010000377         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_95196_length_1692_cov_3.168601, whole genome shotgun sequence.
ACCESSION   DAOQVW010000377 DAOQVW010000000
VERSION     DAOQVW010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95196_length_1692_cov_3.168601"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..894
                     /gene="aroD"
                     /locus_tag="PKK85_07650"
     CDS             196..894
                     /gene="aroD"
                     /locus_tag="PKK85_07650"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNS25929.1"
                     /translation="MNKRKVVVRGFQRKQGQVCASILQENGEDVIKSAATAIKCGADL
                     LEIRLDALTHLDPELVRNILKQIPHPLIATNRMKEEGGFFQGSETERIDALLAATEWA
                     DYVDVELQTRDKYKSEIRETARSTIISYHNFQETPPQDELIRIARKATAEGDLAKLAV
                     MPRNMQDTLLVLELISRCRNTIAISMGKLGSYTRIAAPLFGSPITYASLDGEVAPGQL
                     DIKTTRDILDVFIN"
     gene            898..1545
                     /locus_tag="PKK85_07655"
     CDS             898..1545
                     /locus_tag="PKK85_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HNS25930.1"
                     /translation="MIYLRSRTWLIVGILVLLLLVGTAYWATSSTSVKIVVETNGTVK
                     GSTQPLPLGNVPEDMITQMKDKALEDVQDETSTVESIKVDMENIAKSHGYTAQVTLIS
                     QFGENQLPMPATVRGTSMIPTLQDNQDIIVLKTDKFQVNDLVVAVHPEYGLIVKRVAQ
                     IDGDQVYLKSDNRQTEIIGTETRMVNGMEEVVTIEKTPLDAWLPKKNVVGVVKVY"
BASE COUNT          523 a          349 c          402 g          418 t
ORIGIN      
        1 ctcccatggt ttatagaaat gggggtattt ttcataaagc ataagtagga taatttgtca
       61 tctctttagg aatatgttaa ggttaggctc ttatagattt tcactaggga tggtagatag
      121 tttttagttt agtaattata attattccct ccaacaaaga tattaagagg tagaaattaa
      181 cctaggatgc ccatcatgaa taaaagaaag gtagtagtca ggggttttca aagaaaacag
      241 ggacaagtat gtgcctcgat actccaagaa aatggtgaag atgtgattaa gtccgcagcc
      301 actgccatta aatgtggggc ggatcttttg gaaatacgtt tagacgcact tactcacctg
      361 gatccagagt tggttaggaa tatcttgaaa cagattcccc atcccctaat cgccaccaac
      421 cgtatgaagg aggagggtgg cttcttccag gggtcagaaa cggaaagaat agatgcacta
      481 cttgcagcta cggagtgggc ggattacgtt gatgtggaac tacagacccg ggacaaatat
      541 aaatccgaaa ttagagaaac tgcccggtcc accataatat cctaccacaa cttccaggaa
      601 acaccccccc aggatgaact tatcaggatt gccaggaaag ccacagcaga gggggacttg
      661 gccaagctgg ctgtgatgcc ccggaatatg caggacaccc tccttgttct ggaactgata
      721 tcccggtgta ggaacaccat cgccatctcc atgggaaaac tcggaagcta tacccgtata
      781 gcagctcccc ttttcggttc accaataacc tatgcatcct tagatggtga agtagctcct
      841 ggacaactgg atataaaaac tacccgtgat attttagacg tattcataaa ttaaaaggtg
      901 atatatttga gatcaaggac atggctcatt gttgggattt tggtgctatt gttactggtg
      961 ggcacggcct actgggctac atccagcacc agtgtaaaga tagtggtgga aaccaatggt
     1021 actgttaagg gatccacaca gcccctgccc ctgggaaacg tgcctgagga catgataacc
     1081 cagatgaagg ataaagcctt ggaagatgtc caggatgaaa ccagcaccgt ggagagcatc
     1141 aaagtagaca tggagaacat agccaaaagc catggctaca ctgcccaggt aactctgata
     1201 tcccagttcg gagaaaacca gctgcccatg ccagccacgg taaggggaac atctatgata
     1261 cccaccctgc aggataatca ggatataatc gtactaaaaa cggacaaatt ccaagttaac
     1321 gaccttgtgg tggctgtcca tcccgaatac ggtcttatag tgaagagggt ggcccagata
     1381 gatggtgacc aggtttatct caagagtgac aaccgtcaga ctgagatcat aggcaccgaa
     1441 accaggatgg tgaatgggat ggaagaagtg gtaaccatag aaaaaacacc cctggatgct
     1501 tggcttccta aaaagaatgt ggtaggggtg gtgaaagtct actgacccct agctatgatt
     1561 atttcagggg acaaaaagaa ggtgaacatt atttttttaa ctctatgaaa aaatagttat
     1621 aaatctagtc cctcattttt ttcttcttga aagaagacat agttaccgat attttcctgt
     1681 gttgaggtta tc
//
LOCUS       DAOQVW010000378         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_95206_length_1692_cov_2.976176, whole genome shotgun sequence.
ACCESSION   DAOQVW010000378 DAOQVW010000000
VERSION     DAOQVW010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95206_length_1692_cov_2.976176"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1015
                     /locus_tag="PKK85_07660"
     CDS             263..1015
                     /locus_tag="PKK85_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide reductase"
                     /protein_id="HNS25931.1"
                     /translation="MAFYTLGLTFWIVIALTVVVLGIWIYGLYFNFKKWGMGSTGYQD
                     ELRHSFWLFIATWIHEAFKDGIWVFVKTLILDVLLLRRTLRRSPVRWIMHMAMFYGFL
                     TLAALSGLGLFMDIIEHFNLLGLAHQAEMVKGYMELPFDLFGYLLLFGSTIAVLRRFI
                     LREVRSRTSAYDVVLLGGVFLITITGFYAEWLRGNSFLIGNAFANPVYAPHFSLLHTI
                     LALALFVVILPWSKYMHVIAAPLTILANRGGE"
     gene            1017..>1692
                     /locus_tag="PKK85_07665"
     CDS             1017..>1692
                     /locus_tag="PKK85_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNS25932.1"
                     /translation="MAEEKKSPAGKPLISGFLKSTQLMELDGCTRCQECMKWCPTFDV
                     RQDRPEITPMYKIAKFRELLGSQYGLRAKLFGPKTFNEDEINRFTEDTYYCTTCGVCG
                     TVCESGINTVELWEAMRPNLVARGNGPYGKQSFFPALLGKDRNPFQAKQEERLCWVPK
                     DATVLESSEIAYFAGCTAAYRQQALAVASVRLMNALDIPFCMLGKDEWCCSSALVRTG
                     QRNIMKE"
BASE COUNT          371 a          478 c          475 g          368 t
ORIGIN      
        1 tctctatcgg accttcatgc tccagcttcg gaagctatat gagggtccat gagggttcag
       61 gcgacgttca aaggatatct cgaacggccc tccggatcgg gccggtttga ggccggggag
      121 gctgcaagat ccgccggaga tattattcgg tatcaaaaat gaccagaagg gaacgtttaa
      181 gtattagttt ctcaggtagg ttactctcaa atggaaagga tggtcggatg gagtaggtgt
      241 ggtttaaaaa ggaggataaa aaatggcgtt ttacaccctt ggtctcacct tctggattgt
      301 gatcgccctt accgtagtgg tattgggaat ctggatctac ggcctctact ttaacttcaa
      361 aaagtggggg atgggatcca ccggttatca ggacgagctg cgacacagct tctggctatt
      421 tatcgcgacg tggattcacg aggccttcaa agatggaatt tgggtattcg tgaagaccct
      481 catcctcgac gtcctccttc ttaggaggac cctcaggagg agccccgtca ggtggatcat
      541 gcatatggcc atgttctacg ggtttttgac cctggccgcc ctttcgggcc tgggcctctt
      601 catggacata attgagcact ttaacctcct gggcctcgcc caccaggctg agatggtgaa
      661 gggctatatg gagctgccct tcgacttatt cggctacctc ctcctcttcg ggtccactat
      721 tgccgtcctg agaaggttca tcctccggga ggtcaggtcc cggacctcgg cctacgacgt
      781 ggtcctcctc ggcggagtat ttctgatcac catcactggg ttctacgcgg agtggctgcg
      841 gggcaactcc ttcctaatcg gaaacgcgtt cgctaacccc gtatatgcac cccacttctc
      901 cctgctccac acgatactgg cgctggcact cttcgtcgtg atccttccct ggagcaagta
      961 catgcacgtt atagctgcac cgttgacgat tctagcaaac agaggaggcg agtaggatgg
     1021 cagaagagaa gaagagtccc gcaggaaagc ccctcatatc aggattcctc aagtccaccc
     1081 agctgatgga gctggacgga tgcacccgat gccaggagtg catgaagtgg tgtcctacct
     1141 tcgacgtaag gcaggaccgc cccgagatca cccccatgta caagatcgcc aagttcaggg
     1201 agctcctcgg gagccagtac ggcctccggg ccaagctatt cggccccaag accttcaacg
     1261 aggacgagat caacaggttc acagaggata cctactactg caccacctgc ggcgtctgcg
     1321 gcaccgtctg cgagtccggg atcaataccg tcgagctctg ggaggcgatg aggccaaacc
     1381 tcgttgcaag aggaaacggc ccttacggaa agcagtcctt cttccccgcc ctcctcggaa
     1441 aggacagaaa ccccttccag gcaaagcagg aggagaggct ctgctgggta cccaaggacg
     1501 ccaccgtcct ggagtcgtcg gagatcgcct acttcgcggg ctgcaccgca gcctaccggc
     1561 agcaggccct cgctgtggca tccgtccggc tgatgaacgc cctggacata cccttctgca
     1621 tgctcggaaa agacgagtgg tgctgctctt cggctctggt gaggacgggc cagcggaaca
     1681 tcatgaaaga ac
//
LOCUS       DAOQVW010000379         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_95208_length_1692_cov_2.866830, whole genome shotgun sequence.
ACCESSION   DAOQVW010000379 DAOQVW010000000
VERSION     DAOQVW010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95208_length_1692_cov_2.866830"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKK85_07670"
     CDS             complement(<1..335)
                     /locus_tag="PKK85_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     formyltransferase"
                     /protein_id="HNS25933.1"
                     /translation="MTRLNWDKVDDTYAEAFSGIFCRTLVTADDEETLTRAAYDATST
                     PGAVIGRVEGGIESWVSPKHTPDGRKGVLLQFWYGSDDLEKFELELSYRIRQDILVKP
                     FTTLFDASHS"
     gene            complement(336..1391)
                     /locus_tag="PKK85_07675"
     CDS             complement(336..1391)
                     /locus_tag="PKK85_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25934.1"
                     /translation="MINYLYTGGGFEVGQRFLVSDCEGPLSLNDNAFELAGHFIPQGE
                     EFFQIVSRYDDVLVEMGRPGYNAGNTLKLIAPFLKAYGASNQNVETFCQENVLLVPGA
                     QKTLTFFLELLPSFIVSTSYQQYLEALCKVTGFPLENCYYTPLDLEKQEISHDEKDQM
                     RKLKKSAVENPGFENLERIFWEKLPQMKISSLMDDIKPVGGAAKKEAVEDIMSRFGYR
                     ASDLIYIGDSITDVEPLRFAKDEGGLAVSFNGNDYALDEAMVAVISSNTLILSLLADL
                     FKREGPEEVLEFADSYAAEPEAALKKQCVKSELVAKLPSTHTFLEVVTSKNRDRLKAE
                     SGKFRRYMRGESIGGLG"
     gene            complement(1384..>1692)
                     /locus_tag="PKK85_07680"
     CDS             complement(1384..>1692)
                     /locus_tag="PKK85_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25935.1"
                     /translation="CVSCGRCVEVCPVEAIRLSEEFFQAEEGRILFKRRTVEGPQSGE
                     VTIDQLSCQACGVCVNKCPADAMTLEDDVVSVDPEKCIVCGKCESICPVGAVRLIVDD
                     "
BASE COUNT          404 a          481 c          372 g          435 t
ORIGIN      
        1 gagtgtgatg catcgaaaag tgtggtgaag ggtttcacca ggatgtcctg cctgatcctg
       61 taggagagtt ccaactcgaa cttctccaga tcatcactac cataccagaa ctggagaaga
      121 acaccctttc taccatcagg tgtatgcttt ggactaaccc aagactcgat ccccccctcc
      181 accctgccaa taactgcgcc gggcgtggat gtggcgtcgt aggcggctcg tgtgagggtt
      241 tcctcatcat cagcagtaac cagggttcgg cagaatattc cgctgaaagc ctcggcgtag
      301 gtgtcatcca ccttatccca attcagcctg gtcattcaac ccaaaccccc aattgattct
      361 ccgcgcatat accttcggaa ctttccactt tctgccttca agcggtcccg gttctttgag
      421 gtcaccacct ccaggaaggt gtgggtggag gggagtttag caaccagttc actcttaacg
      481 cactgttttt taagagctgc ttcaggttct gctgcataac tatctgcaaa ttccagtact
      541 tcttccggac cctcccgttt aaagagatcc gccaacaagg acaaaatcag ggtgttgctg
      601 gatataactg ccaccatggc ctcatccagg gcgtagtcat taccattgaa ggacacagcc
      661 aagccaccct catctttggc gaagcgaagt ggctccacat cggttatact gtcaccgatg
      721 tagatcaagt ccgaggcccg gtagccaaag cggctcatta tatcctccac tgcctctttt
      781 ttagcggcac cccccactgg cttaatatca tccatcaggg aactgatctt catctgtggc
      841 agtttttccc agaagatgcg ctccagattt tcaaatcccg gattttctac cgcggatttt
      901 ttaagcttcc tcatttgatc cttttcatca tggcttattt cctgtttctc cagatccaga
      961 ggggtgtagt aacagttttc cagggggaac ccggttacct tgcacagggc ctccaggtac
     1021 tgctggtaac tggtgctgac gatgaaactg ggcagcaact ccaagaagaa ggtcagagtc
     1081 ttctgggccc caggcaccag aaggacattt tcttggcaga aggtttccac attctggtta
     1141 ctggcaccat aggccttcaa aaatggagcg atcaatttca gggtgttgcc agcgttgtat
     1201 cctggtcttc ccatctccac cagaacatca tcgtagcggc tcacgatctg gaagaattcc
     1261 tcgccctggg gtatgaagtg cccagccagt tcaaaggcgt tgtcatttaa tgataggggt
     1321 ccttcacaat cggataccag aaatctttgg cccacttcaa atcctccacc agtatacaaa
     1381 taattaatca tcaactatta atcgaacagc acctaccggg cagatggact cgcatttacc
     1441 acatacaatg catttttccg gatcaacact caccacgtcg tcttccagag tcattgcatc
     1501 agcagggcac ttgttaacgc agaccccgca tgcctggcaa cttaactggt caatggttac
     1561 ttcaccactc tgcggaccct ccactgtacg ccttttaaag agtatcctgc cctcctcggc
     1621 ttggaagaat tcttcactta acctgatggc ctcaactggg cagacctcta cgcaccggcc
     1681 acaggatacg ca
//
LOCUS       DAOQVW010000380         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_95364_length_1690_cov_3.557798, whole genome shotgun sequence.
ACCESSION   DAOQVW010000380 DAOQVW010000000
VERSION     DAOQVW010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95364_length_1690_cov_3.557798"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PKK85_07685"
     CDS             complement(<1..471)
                     /locus_tag="PKK85_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNS25936.1"
                     /translation="MQKFRCVICDYIYDPEKGDPESGAEPGTSFEDLPDDWLCPLCGV
                     GKDQFVPVEEEKPKETKSKKETLDMFCYQCSQTARGTGCTVKGVCGKEPTVARLQDNL
                     LFAIKGISAYLYHARELGYSDDEVDAFLEKSFFSTLTNVNFDAGNFVDLALEAGH"
     gene            707..>1690
                     /locus_tag="PKK85_07690"
     CDS             707..>1690
                     /locus_tag="PKK85_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS25937.1"
                     /translation="MKLNNITPDSVLSDQKKSTNLEEAKKECKLVVFQSIGYPFLCNL
                     VESPKIEIFDKELFELYAQDQWDGFTANEGSFLFDQKLLPDFAFKIIKAHPENSRITK
                     NTNILLMELPEVREIKRVESKILMEDVIGQERAKTKCKIIMKYLQDPEIFGEWAPRNV
                     LFHGFPGTGKTMLAKSLSSELKIPLFMVKATTLIGEHVGDGARQIHDLFEMAASCAPS
                     VIFIDEIDAIGLDRRYQSLRGDVSEVVNALLTEMDGISPNLGVVTIGATNNPPLLDQA
                     LRSRFEEEIEFTLPDESERLSILELYSVSLPLEIDIDLKKLATITQGISRRD"
BASE COUNT          507 a          356 c          344 g          483 t
ORIGIN      
        1 gtgacctgct tccagagcca ggtccacgaa gttacctgca tcgaagttaa cgttggtgag
       61 ggtggagaag aaacttttct ctaggaaggc atccacttcg tcatcactgt aacccaattc
      121 tcgggcatgg tagaggtaag ctgatatccc cttaattgcg aataacaagt tatcctgtaa
      181 acgagccacg gtgggttctt ttccacagac tcccttaact gtacatcccg tgcccctggc
      241 agtttgagaa cactggtaac agaacatgtc tagagtttct ttcttgcttt tcgtttcttt
      301 gggtttttct tcttctacag gcacgaactg gtcttttccc acaccacaga ggggacatag
      361 ccagtcgtca ggcaaatcct cgaagctggt tccgggttct gcaccactct ctgggtctcc
      421 tttttcaggg tcatagatat aatcacatat cacgcatcga aatttttgca tacttatcac
      481 catttttttg ttcggcataa gccgaatgtt ctgttatatc cgaacaatct atatataaat
      541 cttgtggttc caactattat ataccactaa agagaatttt ctttggagaa aagaacttgg
      601 ggaaaatatt tatcttggct tatcaagaaa taaaagtaaa ttctaaattt tgttcatgga
      661 ttaaactaag tttaacgctt aaaaaatata tcggcaggta ttctatttga aacttaacaa
      721 cataactcca gattccgtat tatcagacca aaaaaaatca accaatcttg aagaagcaaa
      781 aaaagaatgt aaattagtgg tttttcaatc cattggctat ccatttctgt gcaacttggt
      841 ggagagtcct aaaattgaaa tcttcgataa agaacttttc gaactctacg ctcaggatca
      901 gtgggatgga ttcacagcaa acgagggttc tttcctgttt gaccagaaac tgttacctga
      961 ttttgcattt aaaataatta aagcccatcc tgaaaattca cgaatcacca aaaacaccaa
     1021 catattgctg atggaacttc ctgaagttcg tgaaataaaa cgagttgaaa gtaaaatcct
     1081 aatggaggat gtaattggcc aagaacgagc caaaactaag tgcaagatca tcatgaagta
     1141 tctgcaggac ccagaaatat ttggggaatg ggcgcctaga aatgttctat ttcatggttt
     1201 tcctggaacc ggtaagacca tgctagccaa atccctttca agtgagctta aaatcccgtt
     1261 attcatggtt aaagctacca ctctgattgg agagcatgtt ggcgatggag cccggcagat
     1321 acatgactta tttgaaatgg cagccagttg tgcaccttcg gtgatcttca tcgacgagat
     1381 agatgccata ggcctggaca gacgctatca atcccttcga ggggatgttt ctgaggtggt
     1441 aaatgctctc ctcacagaga tggatggaat aagccccaac ctgggagtgg taaccattgg
     1501 tgccactaac aatccgcccc ttctggatca ggcattgcgg agtcgatttg aagaggagat
     1561 tgaattcacc ctacctgatg aatcggaaag attatccatc ctggaactgt actccgtctc
     1621 cctgccatta gaaatagata ttgatcttaa aaaattagct acaattaccc agggaatatc
     1681 tagacgagat
//
LOCUS       DAOQVW010000381         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_95482_length_1689_cov_3.155447, whole genome shotgun sequence.
ACCESSION   DAOQVW010000381 DAOQVW010000000
VERSION     DAOQVW010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95482_length_1689_cov_3.155447"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1689)
                     /locus_tag="PKK85_07695"
     CDS             complement(<1..>1689)
                     /locus_tag="PKK85_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNS25938.1"
                     /translation="VMVFESFDALSRFSWNTLREVDLLIVDEFHMIGEYSRGPTIECA
                     ITRSRILSKGMRIIALSATLQNMEELSGWLEARVVEHDYRPVPLHKEVLNMEEFHLKN
                     KNDVILRILKESLDESSQSLVFVSTRRFTESLAGYLAGKIRKNLPDHKQEEFKRVARK
                     ILDVPKRRGSLPTAVCMKLAESAENGLVFHHAGLFDQQKDIIEEEFRDGNLLMIIATP
                     SLMYGVNLPSRSVVIRDYTRWTSQGLQPIPVFDYEQMSGRAGRPGYDTEGYSYLIAKT
                     MDEGLDLQERYLHGEVETTNSRLLENKDAIFRQIIAQVASGLAKNPIELTEFFQKTFY
                     GHQISGSEYLSLLSADTMEYELKDALEFLLQNGIIQATPDGLRTTDLGMLIARSNYSV
                     QTAVRLREFARSSVELDIPRLVYEICLTPDLPKVSFKGRKSREPVRERLNRAGVFMVD
                     VRNEEATAAVLMEWMNERSEYEMENAFHVYAAGARRTAYEASLLIKFYREICQVLGIY
                     TGLDQLNVLSARMYYGAREDILPLTVSVRGLGRKRARALVDAFGTDLSYVSRKEL"
BASE COUNT          408 a          447 c          389 g          445 t
ORIGIN      
        1 agttccttcc gggagacata gcttaaatcc gttccaaagg catctaccag tgccctagct
       61 ctttttcggc ctaatccccg taccgagact gttaagggca gtatatcctc ccgggctccg
      121 tagtacatgc gggctgacag aacgttcaac tggtccaagc cagtgtagat gcccagcacc
      181 tggcagatct cccggtaaaa tttgataagt aaagaagctt cgtaggcagt tctccgtgcc
      241 cctgcagcat aaacatggaa tgcattttcc atttcatatt cactcctctc gttcatccac
      301 tccatcagca cagctgcagt ggcttcttca ttacgcacat ccaccatgaa gacaccagcc
      361 cggttaagcc tctcccttac aggttctctg cttttacgtc ccttgaaact cactttggga
      421 agatctggag tgaggcatat ctcatacacc agccggggta tatcaagttc cacagaggaa
      481 cgggcgaatt ctctcaaacg cacggcagtc tgcacagagt agttgcttcg ggcaatcaac
      541 atccccagat cagtggtgcg caatccatct ggagtggctt gtatgatccc attctgcaac
      601 aggaactcca gagcatcttt aagttcgtac tccatggtgt cagctgataa aagggaaaga
      661 tattcactgc cacttatttg atggccgtag aaagttttct ggaagaattc agttagttca
      721 atggggtttt ttgccaggcc ggaggcaact tgggcgatga tctgacggaa aattgcgtct
      781 ttattttcca agagccggga gttagtggtt tccacttccc catggaggta tcgctcctgg
      841 aggtcgagtc cttcatccat ggttttggca attaagtaag aatatccttc cgtgtcgtac
      901 cctggtcttc ccgcccttcc cgacatttgc tcgtaatcga agacaggtat gggttggagt
      961 ccctggctgg tccagcgggt gtagtcccgg ataaccacac tgcgactggg caagttcacc
     1021 ccatacataa ggctgggagt ggcgatgatc atgagtaaat taccatcacg gaactcttct
     1081 tcaattatgt ccttttgttg gtcgaagaga ccggcatggt gaaacaccaa cccattttct
     1141 gctgactcag ccagcttcat gcacactgca gtgggtaagg aacccctcct tttcggcaca
     1201 tccaggatct tcctggccac ccttttaaat tcctcctgtt tatggtctgg aaggtttttt
     1261 cgtatctttc cagctagata accggccaga gattcggtga agcgtctagt ggaaacaaaa
     1321 accaagctct gggatgattc gtccagagat tccttcagaa tccgaagtat cacatcattt
     1381 ttattcttaa gatggaattc ctccatgttg aggacctctt tatggagggg cacgggccga
     1441 tagtcatgct ccaccacccg ggcctccagc catccggaaa gttcttccat attctgcaat
     1501 gtagctgata aggcgatgat acgcatcccc ttactcagga tccggctgcg ggtaatggca
     1561 cattcaatgg tgggtccccg gctgtactct ccaatcatat ggaattcatc cacgattaaa
     1621 agatccacct ccctgagggt gttccaggaa aaccgggata aagcgtcaaa ggactcaaac
     1681 accatcacc
//
LOCUS       DAOQVW010000382         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_95570_length_1688_cov_3.238824, whole genome shotgun sequence.
ACCESSION   DAOQVW010000382 DAOQVW010000000
VERSION     DAOQVW010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95570_length_1688_cov_3.238824"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..265)
                     /locus_tag="PKK85_07700"
     CDS             complement(38..265)
                     /locus_tag="PKK85_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14e"
                     /protein_id="HNS25939.1"
                     /translation="MPAIEVGRVCIKTAGREAGQKCVIVEIIDEKFVEVVGGSIKNRR
                     CNIKHLEPTTQSLELKSDDAEEIVKQLESEA"
     gene            complement(265..783)
                     /locus_tag="PKK85_07705"
     CDS             complement(265..783)
                     /locus_tag="PKK85_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS25940.1"
                     /translation="MIITIGGLAGTGTSTVASILSQKMGIPYLSAGDIFRQMAAMRGM
                     DVLDFSKFAEGNTDIDREIDQRQAQKARESENLIVEGRLSAYFVEAPLKVWLTAALDI
                     RSVRISHRENKRVELVREEILTREKSEAQRYQEIHNLDIGDMEVYHLIINTERFDAPG
                     VAQIIEKAVEVI"
     gene            complement(792..1058)
                     /locus_tag="PKK85_07710"
     CDS             complement(792..1058)
                     /locus_tag="PKK85_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34e"
                     /protein_id="HNS25941.1"
                     /translation="MPQLRYRSRSYKRTYKKTPGGKTVLRYKKKKPSKHLCAECGKLL
                     HGVPRGRPYQIKKLAKSKRRPNRPYGGYLCTECTRRIYKQEARS"
     gene            complement(1191..>1688)
                     /locus_tag="PKK85_07715"
     CDS             complement(1191..>1688)
                     /locus_tag="PKK85_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HNS25942.1"
                     /translation="GPILTVFIIAALVAFFITLANKLLVDQDRLEFLQKEMKGYQAEM
                     MAAQKSGDQAAMAKVQKKQAEFMKLQQEMMGNSFKPMLVTMIPILLVFWWMASVPAIT
                     SFYMEVPTITYYTLLVPLFHMLYQPSDFVPAGVMAIEWLGWYILCSFAFSLIWRKFLG
                     LKGGM"
BASE COUNT          311 a          427 c          440 g          510 t
ORIGIN      
        1 tatggagaag cttggtcatg aaggtcctct ctccggttta agcctcggat tccagttgtt
       61 taactatctc ttctgcatcg tctgatttga gttccaggga ttgggtggtg ggctccaggt
      121 gtttgatgtt gcacctgcgg ttctttatgg aacctcccac tacttccacg aatttttcat
      181 cgatgatctc cactatcaca catttctggc cggcttctct accggcggtt ttgatgcata
      241 ctcttcctac ttctattgct ggcatcatat cacctctact gctttttcta tgatctgggc
      301 cacgcctggg gcatcgaatc tttcggtgtt gattatgagg tggtaaacct ccatatcccc
      361 gatgtccagg ttatgtatct cctggtagcg ttgggcctca cttttttctc tggttaatat
      421 ttcttctcgg accagctcca ctcttttatt ctcccggtgg ctgatgcgca cactgcggat
      481 atccagggct gcggtgagcc agactttcag gggtgcctcc acgaagtagg cggataagcg
      541 tccctccaca atgaggtttt cactctcccg ggctttctgg gcctggcgct ggtctatttc
      601 acggtcaatg tcagtgttgc cctcggcgaa tttgctgaaa tccagtacat ccattcccct
      661 catggcagcc atctgacgga atatgtctcc ggctgagagg tagggtatcc ccattttctg
      721 ggacaggatg ctggccacgg tgctggttcc agttccagcc aatcctccga tggtgattat
      781 catatatctc tctaggaccg ggcctcttgc ttgtagatcc ggcgggtgca ctctgtgcag
      841 agatatccac catagggccg gttgggcctt ctcttggatt tggctaattt tttgatctgg
      901 tagggccttc cccgtggcac tccatggagc agtttaccac actcggcaca taggtgcttg
      961 gagggctttt tcttcttgta gcggaggact gtttttcctc ctggggtttt cttataggtt
     1021 ctcttatatg atctggacct gtatcttaac tgtggcattt tagatctttc ctaaacaatt
     1081 ttttttttgt tcgtcaaatt tttttttggt gagcgttaat tggagttggg gctgccagtc
     1141 ctcctggatg tatcaccctg gaggaggggt gcatcccctg tgacttgtcc ctacattccc
     1201 cctttaagtc ccaggaactt tctccagatg agggagaagg caaaggaaca gagtatgtac
     1261 catcccagcc attcaatagc catcaccccg gcgggtacga agtcggaggg ttggtacaac
     1321 atgtggaaga ggggtaccag tagggtgtag taggttatgg ttggcacctc catgtagaag
     1381 ctggttatgg ctggtaccga ggccatccac cagaagacca ggaggatggg gatcatggtc
     1441 accagcatgg gcttgaagct gttgcccatc atttcctgct ggagcttcat gaattcagcc
     1501 tgctttttct ggaccttggc catggcagcc tggtcacctg acttctgggc ggccatcatc
     1561 tcggcctggt agcccttcat ctccttctgc agaaattcca ggcggtcctg gtccaccagt
     1621 agcttgttgg ccagggtgat gaagaaggcc accagggcgg cgattatgaa tacggtgagt
     1681 atgggtcc
//
LOCUS       DAOQVW010000383         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_95678_length_1687_cov_2.982230, whole genome shotgun sequence.
ACCESSION   DAOQVW010000383 DAOQVW010000000
VERSION     DAOQVW010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95678_length_1687_cov_2.982230"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..738
                     /gene="hisH"
                     /locus_tag="PKK85_07720"
     CDS             121..738
                     /gene="hisH"
                     /locus_tag="PKK85_07720"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNS25943.1"
                     /translation="MPTIVDYGSGNLKSISNAFTRIGREVRISRDKEELKDSEALVLP
                     GVGAFGMAMENLMGYQKIILQHINEGKPFLGICLGLQVLFSSSHESPGVKGLDVFPGE
                     VVHFPSIIRRKGLKIPHVGWNKLQIKRCCPILEGVGSDFMYFVHSYYVKPCQEEIVAA
                     SVDYGLEVPCVVFKDNVFATQFHPEKSGEKGLLILKNFLELVDSY"
     gene            805..>1687
                     /locus_tag="PKK85_07725"
     CDS             805..>1687
                     /locus_tag="PKK85_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNS25944.1"
                     /translation="MDIEGFTRQALEGNEEKITQKRLLDKILEYKNISPELAGEMAWA
                     VIDEVKSTLKIKDHPDKDLQELIGYPSAGVAMGEMGVGSRGEGDFFVHRRIADIVKST
                     HTNAFINPTAQDDGGVVRSTLDGEEIYITTAVDGIHSRLSEYPFLGGFHVARAAMRDV
                     CVMGADPVALLSDLHLADDGDVGKLFDYTAGVCAVSELTGVPLVAGSTLRVGGDMVLG
                     DRLVSAVGAVGISAHPPTARKEAQVGDVILLTEGSGGGTITTTAIYHGLFDVVWETMD
                     ISFIKASEAILKAGLLPQ"
BASE COUNT          457 a          351 c          445 g          434 t
ORIGIN      
        1 atagtggcca tggagaacac ggtactgcac gaaatttcaa cacctcaccc caacgatact
       61 gtccgaatca aggatttcta caacatacgt tgaaaactta tcatcaccac cccaaaaaat
      121 atgcccacca ttgtcgacta cggaagcgga aatctgaaga gcattagtaa cgcatttacc
      181 cgaattggaa gggaagttag aatttcccgg gacaaggaag agttgaaaga ttctgaagcc
      241 ctggttcttc ctggggtggg tgcctttggc atggccatgg agaatctgat gggttatcag
      301 aagatcatac ttcagcatat aaatgaaggt aaaccttttt tgggtatctg tctgggttta
      361 caggttttat tctcctccag ccatgaaagt ccgggtgtta aaggattaga tgtgtttccg
      421 ggagaagtag ttcattttcc atcgattatt aggaggaaag gtcttaaaat tccacatgta
      481 ggttggaata agctacagat aaagcgttgt tgccctattt tagaaggagt gggttcggac
      541 ttcatgtact tcgttcattc ctactacgtt aagccctgcc aggaggaaat tgttgctgcc
      601 agcgttgatt atggtctgga agttccgtgc gttgttttta aagataacgt gttcgcaacc
      661 cagttccatc cagaaaaaag tggagaaaaa ggtcttttaa ttctaaaaaa cttcctggaa
      721 ctggttgatt cgtattagtt tgtacaatga tttagtaata aatatttata tattagtaat
      781 aatatattgt aggtgggcac acaaatggat atcgaaggct tcaccaggca ggccctggaa
      841 ggaaatgaag aaaaaataac tcagaaacgt ctcctagata agattctgga gtataaaaat
      901 ataagcccag agctagctgg agaaatggct tgggcagtga ttgatgaagt gaaaagtacc
      961 ctgaaaatca aggaccaccc agataaggat ctgcaggaac taattggata cccatccgcc
     1021 ggagtggcca tgggagagat gggggtggga tcccggggag aaggggactt cttcgtgcac
     1081 cgcagaatag cagacattgt caagagcacc cacaccaatg ccttcatcaa tcccaccgcc
     1141 caggatgatg ggggggttgt tcgatccact ttggatgggg aagaaattta catcaccacc
     1201 gccgtggatg gtattcactc ccgccttagt gagtatcctt tcctgggggg attccacgtt
     1261 gccagggccg ccatgagaga tgtttgtgtc atgggagcgg atccagtagc actgcttagt
     1321 gatctgcact tggctgatga tggtgatgta gggaagctct tcgactacac cgctggtgtc
     1381 tgtgctgttt cagagttaac tggtgttcct ctggtggctg gtagcaccct ccgggtgggt
     1441 ggggacatgg tcttagggga ccgcctggtc agtgcagttg gtgccgttgg gatttcagcc
     1501 caccccccaa ctgcccgtaa agaagcccag gtcggagatg ttatactgtt gacggaaggc
     1561 agtggcgggg gaaccatcac caccaccgcc atataccatg gactctttga tgtggtgtgg
     1621 gaaaccatgg atatcagctt cataaaggcc tcagaggcca ttttaaaggc agggttactc
     1681 ccccagg
//
LOCUS       DAOQVW010000384         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_96454_length_1679_cov_2.905788, whole genome shotgun sequence.
ACCESSION   DAOQVW010000384 DAOQVW010000000
VERSION     DAOQVW010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96454_length_1679_cov_2.905788"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PKK85_07730"
     CDS             complement(<1..552)
                     /locus_tag="PKK85_07730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264807.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HNS25945.1"
                     /translation="MTGIIKKIAQINFGLLSPENIRKMSVTKIVTPDTYDEDGYPIEA
                     GLMDPRLGVIDPGLRCRSCGSKGGDCQGHFGHINLARPVIHVGFADTIHKILRSTCRN
                     CGRILLTDREILDYKEKIDNFLRNEENITNVLKEIYNLARKDDCPHCHQSETVRDNSE
                     KLMEKFEDLVKEIEGSGLKVDSKI"
     gene            complement(586..>1679)
                     /locus_tag="PKK85_07735"
     CDS             complement(586..>1679)
                     /locus_tag="PKK85_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25946.1"
                     /translation="EETPFIPVTDALDAEFLKAPPFIPFDLPILELLRMPARNCPDSI
                     SAQLEYIRDNWQSILPPRFLEEIDEFGTVAERRRETSVTVRHGERGVVDAVLLTETVE
                     GSKLAKIRVRDQRQPEFGDKFASRHGQKGVVGLIVPQENMPFTEDGVVPDLIINPHAI
                     PSRMSVGQVLEMLSGKAGCLEGQRMDGTPFSGNHELEVMELLRENGFETAGRESMYNG
                     ITGERIESEIFIDVTYYQKLHHMTSDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEM
                     ERDCLIAHGAALALKERLLDESDKYDAIVCSECGMVAIYDRIRDKKYCSICGESDTFP
                     VEISYAFKLLLDELKSLCIFPKLVLEDKA"
BASE COUNT          398 a          441 c          360 g          480 t
ORIGIN      
        1 gatttttgaa tctactttca gaccagaacc ctcaatttct tttacaaggt cttcgaattt
       61 ttccatcaat ttttctgagt tatccctgac agtttctgac tgatgacagt gagggcaatc
      121 gtctttacgg gctaagttat aaatttcctt tagaacatta gtgatgtttt cttcatttcg
      181 caggaaatta tcaatttttt ccttataatc aagtatttcc cggtctgtta acaatattct
      241 tccacagtta cgacaagtag acctcaaaat tttatgtata gtatctgcaa atccaacgtg
      301 gataactggc cgggccaagt taatatgtcc aaaatgtccc tggcagtccc cacccttaga
      361 tccgcaggaa cggcaccgga gcccaggatc aattaccccc agacgaggat ccattaaacc
      421 cgcctcaatg ggatatccat cctcatcata ggtgtcggga gtcacgatct tggtgactga
      481 catcttacga atattctccg gggaaagcag gccaaaatta atctgggcaa tcttttttat
      541 aattcctgtc aaactaatct ctccttatcc tcttacaccc taaattcaag ccttatcctc
      601 cagaaccagc ttagggaata tgcacaggct cttgagttcg tccaaaagca atttaaatgc
      661 gtatgaaatc tcaactggga atgtatctga ttctccgcag atggaacagt actttttatc
      721 ccttatccga tcgtagatag ctaccatccc gcattccgag catacaatgg catcatactt
      781 atcagattca tctaaaagtc tttctttaag tgctaaagca gccccgtgag ctattaaaca
      841 gtctctttcc atctcaccaa atcggagccc cccctcacga gctcttccct ctgttggttg
      901 tcgggttaga acttgcactg gcccacggga acgggcgtat accttgtcag atgtcatgtg
      961 gtgcagtttc tgatagtagg tcacgtctat gaaaatttcc gattctatcc tttcaccggt
     1021 gatcccgttg tacatggatt ctcggccggc tgtttcgaat ccattctccc gtagaagttc
     1081 catgacttcc agttcatgat ttccactgaa gggtgttcca tccatgcgct gtccttccaa
     1141 gcaaccagct ttaccagaaa gcatttccag cacttgcccc actgacatac gggaggggat
     1201 ggcgtggggg ttgattatca ggtcgggaac taccccatcc tcagtgaaag gcatgttttc
     1261 ctggggaact atgagcccca ccaccccttt ctgtccatgt cgggatgcga atttatcccc
     1321 aaactcgggc tgtctctggt ctcggactcg tatcttggcc agtttactac cctccacggt
     1381 ttcagttagt aacactgcat ccaccactcc acgctctccg tgacgcacag ttaccgatgt
     1441 ttcacgtcgc ctttcagcca cggttccgaa ttcatctatc tcctccagaa accggggggg
     1501 gaggatggac tgccagttgt ccctgatata ctcgagctgt gccgagatgg aatcggggca
     1561 gttcctggcc ggcatccgca ggagctccag gatgggcagg tcgaacggga tgaagggggg
     1621 ggctttcagg aattccgcgt cgagggcgtc tgtcaccggt atgaatggtg tttcctcgt
//
LOCUS       DAOQVW010000385         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_96814_length_1675_cov_3.577160, whole genome shotgun sequence.
ACCESSION   DAOQVW010000385 DAOQVW010000000
VERSION     DAOQVW010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96814_length_1675_cov_3.577160"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKK85_07740"
     CDS             <1..410
                     /locus_tag="PKK85_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNS25947.1"
                     /translation="LNVDKQKIEFTITAGGKIFPEVPEVIKELKNRGIHIYVASGDRK
                     KTLEQLADFIDIPCENSFGTADAWEKREIISGLQKKHRVMMVGNSANDILALKEADIG
                     VLTLQQGEIVPSKVFEAADVVVHNIKEILDIEF"
     gene            complement(601..1674)
                     /locus_tag="PKK85_07745"
     CDS             complement(601..1674)
                     /locus_tag="PKK85_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNS25948.1"
                     /translation="MSRGKDDSKVKKLERLFNRAKLGTKIRENDLVGIKLHFGEEGND
                     SYINPVFVRIIVDMVRKQKAKPFLTDTNTLYHGSRHNSVDHLETAIKHGFDYSVSGAP
                     IVIADGIRGDNWVTVELGLKHFEKVKIAGDILNSDSLIVLSHFKGHGMSGFGGAIKNL
                     AMGCAAIPGKLEQHECAKPIVASSCSGCGTCVGSCPVSAISLYEGRAVIEYQACIACN
                     NCLDICPDSCLELDWAAMKPFMEKMAEYAWGAVKNKHDRMGYVNFLLNITPDCDCVSW
                     SDRPLVPDIGILASTDPVALDQASYDLVNQQPGLENSLLIHCHAPGEDKFRGVWENVD
                     GLILLEYAQKIGLGSRNYELITI"
BASE COUNT          521 a          360 c          325 g          469 t
ORIGIN      
        1 tactcaacgt ggataaacaa aaaatcgagt tcaccatcac tgccggaggc aaaatatttc
       61 cagaagttcc agaagtcata aaggaactca aaaatagggg aatccatata tatgtggctt
      121 caggagatag gaaaaaaacc ctagaacagt tagctgattt tattgacatc ccctgcgaga
      181 atagctttgg cacagctgat gcctgggaaa agagggagat catcagtgga ctgcagaaga
      241 agcaccgggt gatgatggtt ggaaacagcg ccaacgatat actggcacta aaagaggcgg
      301 atattggtgt tttaactctc caacaaggag aaattgtgcc gagtaaagta tttgaagcgg
      361 ctgatgtggt tgttcataac attaaagaaa ttttagatat tgaattttaa ttcaaagcca
      421 gtgaatagat taattaattt ctactgtaga agaagtgggc ttgatttttt acacaacacc
      481 caacaatgat tctacagtag aaaaaatatc tattatacag ctattttttc aacaattata
      541 aaaaaaataa tgtaaaagag gcttaaaata ttgtaaagga taatcgaagg gttttttaac
      601 ttagattgtg atcaattcat aattcctact tcccaatccg attttttggg catactccag
      661 taaaatcagc ccatctacgt tttcccacac cccccggaat ttgtcttcac caggggcatg
      721 acagtggatt agtaacgaat tttctaggcc tggttgctgg ttaaccaagt catagctggc
      781 ctgatccaga gccaccgggt cagttgatgc caggatacca atatctggaa ccaggggacg
      841 atcactccat gacacacagt cacaatccgg tgtaatgttc aagaggaaat tcacatatcc
      901 catcctatca tgtttgtttt tcacagcccc ccaagcatat tcagccattt tctccataaa
      961 tggtttcata gccgcccagt ccagttctaa acaggaatca ggacaaatat ccagacaatt
     1021 gttacaagct atacaagcct ggtattcgat gactgctcgg ccctcataaa gggaaattgc
     1081 cgaaacagga cagctaccca cgcacgttcc acagccactg caggatgaag caacaatagg
     1141 tttggcgcac tcgtgttgtt ccaactttcc agggattgca gcacacccca tggccagatt
     1201 tttaattgcc ccaccaaatc cactcatacc atgaccctta aagtgggata aaacaattaa
     1261 actatctgaa tttaatatgt ccccggcaat ttttactttt tcaaaatgtt taagacccaa
     1321 ttcaactgtt acccagttgt cgccgcgtat tccatcagca attactatgg gggctccgga
     1381 gacggagtag tcaaatccat gttttatggc agtttccaga tgatccacgg agttgtgtcg
     1441 actaccatga tacagagtat tggtgtcggt taaaaaaggt ttagctttct gctttcgaac
     1501 catgtcaaca atgatccgaa caaaaacagg attgatgtat gaatcattgc cttcttcacc
     1561 aaaatgtagc ttgatgccca ctaaatcatt ttctcggatt tttgttccca gttttgcccg
     1621 gttaaataat ctttccagtt ttttaacctt gctatcatct tttcccctgg aaata
//
LOCUS       DAOQVW010000386         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_97069_length_1673_cov_2.120519, whole genome shotgun sequence.
ACCESSION   DAOQVW010000386 DAOQVW010000000
VERSION     DAOQVW010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97069_length_1673_cov_2.120519"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKK85_07750"
     CDS             complement(<1..358)
                     /locus_tag="PKK85_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNS25949.1"
                     /translation="MMILDEVLKANQEFVKEFEPKKLSHSPQKKLAIVTCMDTRLTGF
                     LEPAMGIGRGDAKIIKNAGNTIVDRDVIRSVAAAIYALGAEEVMVVGHYECGMANVDP
                     EKLISAMKARGIPEEIL"
     gene            complement(598..>1673)
                     /locus_tag="PKK85_07755"
     CDS             complement(598..>1673)
                     /locus_tag="PKK85_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNS25950.1"
                     /translation="VLAVKKGLDLYDAVPTLITDPDKVIESAGSLHCGTLNIGKTIVR
                     YLDGAKDLKLAVTTKPCDAMTIVELMKRKQIERDNVVMIGVNCGGTLPPVKAREMVEK
                     FYEIDPDMVVKEEIAGGKLIIETADNEEKEISIDDLEDEGYGRRTNCRRCENNIPRMS
                     DIAMGNWGVIGPLAGKATFIEVFSEKGAEVLEKAIKAGALRVQDPVPKGVEIRANIDK
                     TMVKLANKWQTRNFEDEGTLITIDQYMDEFSKCIKCYGCREACPLCFCRDCSIQSESN
                     NWVEKGIIPPSPMFHFVRMLHMVDSCTNCGQCEEVCPSEIPLARIFHEINVKLQEEFD
                     YKPGYDLEQKPPLSVTDKEPSEE"
BASE COUNT          367 a          392 c          326 g          588 t
ORIGIN      
        1 aaagaatttc ctcagggata cctctggctt tcatggcact tatgagtttt tcagggtcta
       61 cgttggccat tccgcattcg tagtgtccta ccaccataac ctcttccgca ccaagtgcgt
      121 atattgccgc tgcaacggat cgtataacat ccctgtcgac tatggtgtta cctgcatttt
      181 tgattatctt tgcgtctccc ctgcctattc ccatggctgg ttctaaaaaa ccggtgagcc
      241 gtgtatccat acaggttaca atggccagtt tcttttgtgg ggagtggctt agtttttttg
      301 gttcaaattc tttaacaaat tcttggttgg ctttgagtac ttcgtcgagt atcataattt
      361 atcaccttaa attgtatcat cctctaaata ccttaaattg tattcctaaa tgccttaatt
      421 ctctaaattt accataaatt gtatcctcta agataaagta aaccgcattt actatgggtg
      481 atctattatt ctcatttaaa gaggtgatta ctagaatttg agcgaatttt tctagtgaat
      541 atgatataat ctatcaccct ggaaatgcgg ttactctacc tatggggctg aggtttttca
      601 ttcctcactt ggttctttat cagttacaga aagtggcggt ttttgttcca ggtcgtatcc
      661 tggtttgtaa tcaaattctt cctgtaattt gacgtttatt tcatggaaga ttctggccag
      721 tggtatttca ctggggcaga cttcttcgca ctgtccacag tttgtgcagg agtccaccat
      781 atggagcatt cttacaaagt ggaacatggg tgatgggggt attattccct tttccaccca
      841 gttgttggat tctgactgga tggaacagtc cctgcagaaa cagagtgggc atgcttctcg
      901 gcaaccataa cattttatgc acttggagaa ttcgtccatg tactggtcga ttgttatcag
      961 ggtgccttcg tcttcgaagt tcctggtctg ccatttattc gccagtttta ccattgtttt
     1021 gtctatgttt gcacgtattt caacaccttt aggtacagga tcctgtaccc ttagtgctcc
     1081 tgcttttatg gctttttcca ggacctctgc acctttttct gaaaacacct cgatgaaggt
     1141 tgctttccca gctaaaggcc ctatcactcc ccagttaccc atggctatat ctgacatcct
     1201 tggtatgtta ttttcacatc tccggcagtt ggtcctgcgg ccgtatcctt catcttccag
     1261 atcatctatg cttatctcct tctcttcgtt gtctgctgtt tctattatca gctttccccc
     1321 ggcaatctct tccttcacca ccatgtctgg atcaatttca tagaactttt ctaccatttc
     1381 acgggccttc accgggggta aagtaccgcc gcagttaact ccaatcatga ccacgttgtc
     1441 tctttctatc tgttttcttt tcatgagttc tactatggtc attgcgtcac atggtttagt
     1501 ggtgactgct aatttcaggt cctttgcacc atcaaggtac cggacaatgg ttttgccaat
     1561 gtttaacgta ccgcagtgca gtgaccctgc tgattctatg accttatcag gatcggttat
     1621 gagggtaggt acagcatcat acaggtctaa acctttcttt acagccaata ctg
//
LOCUS       DAOQVW010000387         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_97142_length_1672_cov_2.813234, whole genome shotgun sequence.
ACCESSION   DAOQVW010000387 DAOQVW010000000
VERSION     DAOQVW010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97142_length_1672_cov_2.813234"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..564)
                     /locus_tag="PKK85_07760"
     CDS             complement(121..564)
                     /locus_tag="PKK85_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25951.1"
                     /translation="MTETDISARLKAWVKREKEPSRASFAVAIIFNLIFIYIINQIPY
                     WDISFITPAWSETLWILNLSLVVAIVGNLLFLFYHRQWFRSLIQIVMNVFAFWAVKAL
                     YTVFPFSFSHSSLNLVAGLILIIMMVGVFISILVELVKLVASVLD"
     gene            913..>1672
                     /locus_tag="PKK85_07765"
     CDS             913..>1672
                     /locus_tag="PKK85_07765"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNS25952.1"
                     /translation="MAGIVGYGVYIPYYRIKVEEIAKVWGDDPRAISRGLVVQEKSVP
                     SPDEDTATISVEAARNSLKRAKINPQKIGAIYVGSESHPYAVKPTATIVADAVAATPE
                     LTAADLEFACKAGTAGIQVCMGLVDSDAIEYGLAIGADTAQGAPSDALEYTASAGGAA
                     YIIGKGNTLADFEGTYSFTTDTPDFYRREGKPYPRHGGRFTGEPAYFKHVLSAAKGMF
                     EKMGTDASDYDHAVFHQPNGKFYIRAARKLGFNED"
BASE COUNT          502 a          364 c          376 g          430 t
ORIGIN      
        1 ctattgggtt ggaccaggat ggttagggat gataacttct aattggtgga tttatggtca
       61 ttcccatcat caaaaacaac ttgtaccaac aaatggctaa tggagtctgt taatatcatc
      121 tcaatccagc actgaagcca ccaacttcac caactccacc agtatagaga tgaacacccc
      181 caccatcatg ataatgagga tcaggccggc cactagattc agagaagagt ggctgaagct
      241 gaaggggaac acagtgtaaa gtgccttaac agcccagaat gcaaatacat tcattactat
      301 ctggatcaag ctacggaacc actgcctatg gtaaaacaaa aagagtaagt ttcccactat
      361 ggcaaccacc agggataggt tcagtatcca cagggtttca ctccaagcgg gagtgataaa
      421 ggatatatcc cagtagggga tctggttgat gatgtaaata aaaatcaggt taaatattat
      481 agccactgca aaagaagccc ttgatggttc tttttctcgt ttcacccatg ctttcaaacg
      541 ggcagaaata tcagtttcgg tcatttttcc cacacattaa tattgatttt ttttgtatta
      601 taaattgctg caacgcaaaa ataaattcat atcatccaag aaacccatat atcaaggcaa
      661 aaagtccctc tttttccaat tcatgattga taggatagga tctgttcggt gtatatagga
      721 aagtaaatct tgatcaaata gggtacaagg ccagatgcaa ggaacaacgt cgtgggaagg
      781 gtcaagcttt ataaggtttg tgcactatat tttttatcac tgtatttttt agaaagagaa
      841 tttaggattt attcatcgct gaacttaaac taggagtttt gttaataatt tatcaaatgg
      901 ttgtgatatt acatggcagg aattgtagga tacggagttt acatacccta ctaccgaatt
      961 aaggtagagg aaatagccaa ggtatggggt gatgatcccc gtgccatttc ccggggattg
     1021 gtggtgcagg aaaagtctgt accatcccct gatgaggata ccgccactat atcagtagaa
     1081 gcagctcgaa attcacttaa aagagctaaa ataaatccac aaaagattgg agccatttat
     1141 gtagggtctg aatcacatcc ttacgctgtt aaacccacag caaccattgt agctgatgct
     1201 gtagccgcca cccccgaact aaccgcagca gatctggaat ttgcatgtaa ggcaggcact
     1261 gcaggtatac aagtttgcat gggtctggtt gattcagatg ccatcgaata cggactggct
     1321 attggtgctg acactgccca aggagctccc agtgacgctc tggagtacac tgcctcggct
     1381 gggggggctg cctacattat aggtaaaggc aacaccctgg ctgactttga aggcacctac
     1441 agcttcacca ccgacacccc cgacttctac cggagggaag gtaaacccta cccccgacac
     1501 ggaggacgat tcactggtga gccagcctac tttaagcacg tattatcggc ggctaaggga
     1561 atgtttgaga agatgggtac cgatgcctcc gattatgatc atgccgtttt ccaccagccc
     1621 aacggtaaat tctatatccg ggcggcccgg aagctgggct ttaacgaaga cc
//
LOCUS       DAOQVW010000388         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_97582_length_1667_cov_3.678660, whole genome shotgun sequence.
ACCESSION   DAOQVW010000388 DAOQVW010000000
VERSION     DAOQVW010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97582_length_1667_cov_3.678660"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKK85_07770"
     CDS             complement(<1..556)
                     /locus_tag="PKK85_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HNS25953.1"
                     /translation="MVQVNDKYYAWSPDEEIAESGECGGAVTSIMKFLLEEGIVDAVL
                     AVKRGADLYDGVPTLITDPEKIIESAGSLHCGTLNICNPLFDYLEGAKNLKIAVTTKP
                     CDAMSIVELIKRKQIDRNNLVMIGVNCGGTLPPVKAREMIETFYDLDPDSVVKEEIAK
                     GKLIIETADGTEKEIPIDDLEDEGF"
     gene            complement(561..986)
                     /locus_tag="PKK85_07775"
     CDS             complement(561..986)
                     /locus_tag="PKK85_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNS25954.1"
                     /translation="MEWKPKIIVFCCNWCSYGGADTAGTARMQYPPNVRVIRVMCSGR
                     INPLFIFKAFDEGADGVMVAGCHFGDCHYDRGNYACDQRMRALKTVLTTLGIEEGRFY
                     LDWISASEGEKFANTMTMVSEKVKELGPFSWRKNQAEIG"
     gene            complement(1162..>1667)
                     /locus_tag="PKK85_07780"
     CDS             complement(1162..>1667)
                     /locus_tag="PKK85_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS25955.1"
                     /translation="LLKKSYILFKEPIEKGITIRKNIDNDMVKLADKWQDADWEDVKG
                     REIFSALTEYLPDFSRCIKCYGCREACPICYCDDCCLEAHNGPDWLSKGEIPPSPMFH
                     LERMIHMVESCTNCGQCEEVCPSEIPLAKIWHEVNAKVKDNFGYVKGTGENKPPMGFF
                     PMDKMPL"
BASE COUNT          424 a          383 c          309 g          551 t
ORIGIN      
        1 cgaatccttc gtcttccagg tcgtcgatgg gtatttcttt ttcggtacca tcggcggttt
       61 ctataatgag tttacccttg gcgatttctt ccttgaccac gctgtctgga tccagatcgt
      121 agaatgtttc tatcatttca cgagccttaa ctggaggtag agttccacca cagttaactc
      181 caatcatgac taagttattt ctgtcaatct gttttctctt tattagttct acgatgctca
      241 tggcatcgca gggtttggtg gtaacagcta ttttgaggtt ttttgcccct tccaagtaat
      301 caaagagagg gttgcatatg ttaagggtcc cgcaatgtag tgaaccagca gactcaatta
      361 tcttctccgg gtctgtaatt aaagttggca cgccatcata aaggtctgca cctcttttaa
      421 cggcaagaac ggcatctaca ataccttctt ccagtaaaaa tttcattatc gaggtcacag
      481 cacctccaca ttctccactt tcagctatct cttcatctgg agaccatgca tagtatttat
      541 cgtttacttg aaccatttaa tcacccgatt tctgcctggt tcttcctcca cgagaaagga
      601 cctagttctt taactttttc gctaaccatg gtcatggtgt tggcaaactt ttcaccctcc
      661 gaggcagaaa tccaatctaa atagaatctt ccttcttcta taccaagggt ggtcagaact
      721 gttttaagag ccctcattct ttgatcgcat gcatagttcc ctctatcgta gtgacagtct
      781 ccaaagtggc atcctgcgac cataactcca tctgcacctt catcgaatgc tttaaagatg
      841 aaaagtggat ttattctacc agaacacatc actctgatta cgcggacatt cggaggatat
      901 tgcatacgag ccgtgcctgc agtgtctgcc cctccatagg aacaccagtt acaacaaaaa
      961 actattattt taggtttcca ttccattttt ttattctccc ctttttttat ttgattaaac
     1021 aataattttc acaggagtag atccggtacc actcaaatat cttgaataaa tcacatgaat
     1081 gtaaattttt aaaaaattta aaaaataagg aaaaaaagat ttccttattt ttccgttggc
     1141 catgtaacat ctctttgccg attagagtgg cattttatcc attgggaaaa aacccatagg
     1201 tggtttattt tcaccagttc ccttgacgta gccgaagtta tctttaactt tggcgttaac
     1261 ttcgtgccaa atcttggcca ggggtatttc acttgggcag acttcttcgc actgtccaca
     1321 gttggtgcat gattctacca tgtgaatcat tctttccaag tggaacattg gtgatggtgg
     1381 aatttctcct ttactcaacc agtctgggcc attatgtgcc tctagacagc agtcgtcaca
     1441 gtagcagatt ggacaagctt ctctgcatcc gtaacacttt atacatctgg agaagtcggg
     1501 caggtattcg gtgagagcag aaaagatctc tcgaccttta acatcctccc agtctgcatc
     1561 ttgccattta tcagcgagtt taaccatgtc gttgtcgatg tttttcctga tggttattcc
     1621 tttttctatt ggttctttga acaagatata agacttcttc aggagtg
//
LOCUS       DAOQVW010000389         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_98002_length_1663_cov_2.791667, whole genome shotgun sequence.
ACCESSION   DAOQVW010000389 DAOQVW010000000
VERSION     DAOQVW010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98002_length_1663_cov_2.791667"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /gene="tmk"
                     /locus_tag="PKK85_07785"
     CDS             complement(<1..357)
                     /gene="tmk"
                     /locus_tag="PKK85_07785"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNS25956.1"
                     /translation="MYLCLEGIDGSGKSTQLAMLGEWLEKCGLEVLRVYEPTESPPGQ
                     LIREMLTDPRATTPHFQRILGLLFAADRMMLMDRIGEAEKQEKIVISDRSFYSSLAYQ
                     SDPDWILEINRHIKKPD"
     gene            complement(363..1202)
                     /locus_tag="PKK85_07790"
     CDS             complement(363..1202)
                     /locus_tag="PKK85_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNS25957.1"
                     /translation="MNAYLEILRPLNALMAVVAIFLMAIISGNFTGDVFIAGAVVFIV
                     TGAGNSINDYFDHKIDAINKPERPIPSGRIPLRNAGMYSSTLFIVGIILAFGINALLG
                     SIALLSSLLMIWYAYSLKRMLIVGNLAISFLTGLCFIFGGIVVDQVMVSVYLSLFAFL
                     MTMAREIVKDMEDVKGDQAEGATTLPLLYGNAISSKAAAFFMVTASLASPLLYFWGVF
                     TWVYLVVLIPAMVVFLYGAGRVLKDQSVKNTRIVSKQIKMGMGVTFLAFAAGSPYLWS
                     LLG"
     gene            complement(1307..>1663)
                     /locus_tag="PKK85_07795"
     CDS             complement(1307..>1663)
                     /locus_tag="PKK85_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNS25958.1"
                     /translation="DMGSINIGEFSNIQDNCVVHSPAEIGDYVTVGHSAVVHACTVEE
                     NCIIGINSTLLDGALIRKNSIVGAGALVTAGKEFPEASLILGVPARAVRQLGADEIKR
                     IRDNALRYVELSQAKI"
BASE COUNT          459 a          425 c          348 g          431 t
ORIGIN      
        1 gtccggtttt tttatgtgcc gattaatttc cagtatccaa tctggatcgc tttggtaagc
       61 caggctagaa taaaaagagc gatcactgat cacgattttt tcctgcttct cagcttcccc
      121 tatcctatcc atgagcatca tccggtcggc agcgaacaac aagcccagta tcctctggaa
      181 gtgtggagta gtagcccggg gatcagttaa catttcacgg ataagctgtc ccggagggga
      241 ttctgtgggt tcgtaaaccc ggagaacctc taatccacat ttttccagcc actcccccag
      301 catcgcgagt tgagtggatt ttccagatcc atcaattcct tccagacaca ggtacatatc
      361 attcacccta ataaagacca taaatagggg gatccggctg cgaatgccag gaaggttaca
      421 cccatcccca tcttaatctg ttttgagaca attctagtgt ttttgactga ttgatccttc
      481 agtacccttc ctgcaccata gagaaatacc accatggccg ggataagaac taccagataa
      541 acccaggtga aaactcccca aaagtatagc agggggctgg ccagactggc cgtgaccatg
      601 aaaaacgctg ctgccttgga agagatagca ttcccatata ataagggcag agttgtggcc
      661 ccttctgcct gatccccctt cacatcctcc atgtccttca ctatctcccg ggccatggtc
      721 atcaggaagg cgaagaggct cagatatacc gataccatca cctgatcaac cacgatccca
      781 ccaaatatga aacataaccc agttaaaaat gatatggcca gattacccac tattaacatc
      841 cttttaagac tgtaagcgta ccatatcatc agaagtgagc ttaaaagagc aatggatcct
      901 aggagggcgt taataccgaa ggccagtatt ataccaacga tgaacagtgt cgaagaatac
      961 atcccggcgt ttcggagggg aatccgcccg gagggtatgg gtctctctgg tttgttaatg
     1021 gcatcaatct tatggtcaaa gtagtcattg atggagttac ccgccccagt tacgatgaaa
     1081 accaccgcac cggctatgaa tacatccccg gtaaagttac cactgataat ggccattaag
     1141 aagatggcca ccaccgccat aagagcattg agtggcctta aaatctctag gtaagcattc
     1201 atgaaatcac tatcttaaac tgatctaaag ggagattagg agcttattta ggaataatgg
     1261 ctttggtaat tactaatcta tctactgttc atcttctaca aactacttaa atttttgctt
     1321 gggaaagttc aacgtacctg agagcgttgt ccctgatcct cttgatctca tctgccccta
     1381 attgtctcac tgcccgtgca gggactccca gtatcagact ggcttcaggg aattccttac
     1441 cagcagttac cagtgccccg gcccctacta tactgttttt tctaatgagt gctccgtcca
     1501 ggagggtgga gttaataccg atgatacagt tttcctccac ggtgcaggca tgcaccactg
     1561 ctgagtgacc caccgtaaca taatcaccta tttctgcagg ggaatgcaca acacagttat
     1621 cctggatatt tgaaaactca ccaatgttga tacttcccat atc
//
LOCUS       DAOQVW010000390         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_98487_length_1658_cov_3.102308, whole genome shotgun sequence.
ACCESSION   DAOQVW010000390 DAOQVW010000000
VERSION     DAOQVW010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98487_length_1658_cov_3.102308"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..323)
                     /locus_tag="PKK85_07800"
     CDS             complement(108..323)
                     /locus_tag="PKK85_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25959.1"
                     /translation="MDEEMMYEMRLPPGVTQKMVAQVITDFELELKHTESGPILYGKI
                     ENLEKAQDYIIKALNERLRELEEGHVK"
     gene            complement(397..1362)
                     /locus_tag="PKK85_07805"
     CDS             complement(397..1362)
                     /locus_tag="PKK85_07805"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNS25960.1"
                     /translation="MDLESKVKIIKEGTLEVVTPDELKEKLSQETVTAYIGYEPSGKV
                     HLGHAITVKKMMALQKAGVKVKILLADLHAYLNGKGSLEEIKQLSEYNKNCFLALGLS
                     EETEFVMGSSFQTDPHYTILVYQLALSTTLTRAKRSMAQITRDAQDHQVAEVIYPLMQ
                     VVDMLFLEVDLAVGGMEQRKIHMLARDNLPRLGYPAPVCIHTPLLHGTDGSEKMSSSK
                     ENFIAIDDKPEVIRNKVKKCYCPPGELEGNPVLEMAQHFIFTDQKTILIKRPEKFGGD
                     LNLNQTELEQMYSSGELHPLDLKNAVANHLVEILEPVRSYMDNLK"
     gene            complement(1396..>1658)
                     /locus_tag="PKK85_07810"
     CDS             complement(1396..>1658)
                     /locus_tag="PKK85_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25961.1"
                     /translation="SFKLQAISWKDYPQLVKVSDSDMIKKTTVTSITPHKIQVLHPDT
                     YEPLDLDLKPFMDDFKIGDEVEVVEIDHQIHILKSDNKRTRI"
BASE COUNT          405 a          375 c          344 g          534 t
ORIGIN      
        1 cctatcctta acccagatgt gcacctggag tgatcagaac cacatcgaag acattgctga
       61 ggaaatagcc ttggaagtcc tggaatggtt ggaataaaaa aaatctacta cttcacatgg
      121 ccctcttcca gttcacgaag gcgctcgtta agggctttta tgatgtaatc ctgagctttt
      181 tcaaggtttt ctattttccc gtaaaggatg gggcctgatt cagtgtgttt tagttcaagc
      241 tcaaaatctg tgattacttg ggcgaccatt ttctgtgtaa cccctggggg gaggcgcatt
      301 tcgtacatca tttcttcgtc catgaatatc caccacttga ttttttttag ggttgtcctg
      361 gttaattaca tttttcctgt aaactaaatt ataaatttat ttcaaattat ccatgtaact
      421 acgtactggc tctaatattt ctactagatg gttagccacg gcatttttca gatcaagggg
      481 gtgtaattct cctgaactgt acatttgctc tagttctgtt tgattgagat tcagatcccc
      541 tccaaacttt tcaggcctct tgatgagtat ggttttttgg tcggtgaata tgaaatgttg
      601 ggccatctcc agaacaggat tcccttctaa ctcaccaggg gggcagtaac acttttttac
      661 cttgttccgt atgacttctg gcttatcatc aatggctatg aagttctctt tactggagga
      721 cattttttcg gaaccatcag taccgtgcag aagtggggtg tgaatgcaca ccggagccgg
      781 ataacccagg cgaggtaggt tatcacgggc cagcatatgg attttacgct gctccatacc
      841 acccactgcc agatccacct ccagaaacag catgtccact acctgcatca gggggtagat
      901 gacctccgcc acctgatgat cctgggcatc ccgtgttatc tgggccatgc tgcgtttggc
      961 tctggtcaat gtggtagata gggctaactg gtaaaccagg atggtgtagt ggggatcagt
     1021 ctggaaacta ctgcccataa caaattctgt ctcctcagat agtcctaaag ctaaaaaaca
     1081 attcttatta tattctgata gctgtttaat ctcttcaaga ctccctttac cattcaaata
     1141 ggcgtgaaga tctgctaaaa gaattttaac ctttacacct gccttttgca gggccatcat
     1201 cttcttcact gttatggcgt ggcccaggtg aacctttcca gaaggttcgt atccaatgta
     1261 ggcggtaacc gtttcctggc tgagcttctc cttgagttca tcaggagtca cgacctccag
     1321 ggtcccctcc tttatgatct taactttaga ctccaggtcc atcttttcac cttcattatc
     1381 agtatgaata gttgttcaaa ttcttgtcct cttgttatct gactttagta tgtgaatctg
     1441 gtggtcgatc tccaccacct ctacctcatc acctatctta aaatcatcca taaaaggctt
     1501 taaatctagg tccagtggtt catatgtatc aggatgtagt acttgtattt tatggggtgt
     1561 tatactggtt acagtggttt tttttatcat atcagagtca gaaaccttca ctaactgcgg
     1621 gtaatccttc caggaaatgg cctgcagttt aaaagact
//
LOCUS       DAOQVW010000391         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_98715_length_1656_cov_2.884447, whole genome shotgun sequence.
ACCESSION   DAOQVW010000391 DAOQVW010000000
VERSION     DAOQVW010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98715_length_1656_cov_2.884447"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..768
                     /locus_tag="PKK85_07815"
     CDS             427..768
                     /locus_tag="PKK85_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25962.1"
                     /translation="MRMRVIRNMENRTQYYHTGVFRRDEDLVIFPFWEFEAFYQDLKY
                     LHQMVRNLLEYGQNGERGCTPEDGIVYAQNYLERMEELLDKLTSYHVQTEIENFPDKR
                     IKETPQCLLGL"
     gene            770..961
                     /locus_tag="PKK85_07820"
     CDS             770..961
                     /locus_tag="PKK85_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25963.1"
                     /translation="MMVLTARVSDGFPEGHPFQADQEVKVMTVSDFEELQKSIKELIR
                     DKKLLERRLEAMEHSKVIL"
BASE COUNT          533 a          306 c          354 g          463 t
ORIGIN      
        1 aagttttaaa ggggatggtg gatgatgaac gcattacttc ctgatggagg gtattcaagc
       61 cggcagtgat tctattataa aagtatgatc ccccagaaca aaaacgaata ctatattact
      121 aatgacaaca ttcctattaa tgtgtcttgt tggtactggt ttaccattgg aaaaaattaa
      181 caccaagcac ttctaattaa aaccacctaa accaacaatg acacactctt cactattttt
      241 accatttaaa atgttagaaa tgtagttcag gggtgaaatt ttcttttaaa ctttcctcaa
      301 tccaatccag atcctcatac tccgcccttt cctggtaata attttttact tctcatctgc
      361 tctagcacaa ggagtgaatg gatgaaccca atatattgat ccattccctt aaggatggtg
      421 aaccagatga ggatgcgtgt gataagaaac atggagaacc ggacccagta ctaccacact
      481 ggcgtctttc gcagggatga ggacctggtg atattccctt tctgggagtt cgaggccttc
      541 taccaggacc tgaagtacct gcaccagatg gtgagaaacc tcttggagta tggacagaac
      601 ggtgagcggg gatgcacccc ggaggatggc atagtctatg cacagaacta cctggagcgg
      661 atggaggaac tcctggataa actgaccagt taccatgtgc agaccgagat tgaaaacttt
      721 cccgataaac gaataaagga gaccccccag tgtctcctgg gactttaaca tgatggtctt
      781 aaccgcccgg gtcagcgacg gattccccga gggccatccc ttccaggccg atcaggaagt
      841 gaaggtcatg accgtcagtg actttgaaga actgcagaag agtataaagg agcttatacg
      901 ggataaaaaa ctcctagaaa gaaggttgga ggctatggaa cactcaaaag taatattata
      961 agtaaggaag actcctgaaa agggtcaaca accttggttt acgaggtgtt ttagattcgt
     1021 gattatgaca tcggccaaat ctgggagata ttgaataatc tcaatcacga tcaggataaa
     1081 attagagaat ctcgtcattt gatggattct tttgaattaa gaagtgatta tcttcattgg
     1141 acatggttta tgatagttta ataataaaaa acatgtacta ataggaaaaa caggttagga
     1201 tacgtttaaa gttttatatg agcatcctaa aaggcagact caagatattt atgttatact
     1261 gcatttggtt gataatgttt taatcatcac cacctatact cacagtgtgg aaaggaggat
     1321 ccgggttaaa tgaattttga gttgattaca agcacgatat ggaagtggat ggattgttta
     1381 tccacgttat aaaagatcat tcctatgaaa acttcggttg aattagataa cgatgtgata
     1441 cttgattttg accataacag ggttccagtg gtccttgaaa tattaaatgc ttttcgtgtc
     1501 tttaaactcc aaaatatagc ttgaataaca tccaaaaggt ttggatgacg gtgaaggttg
     1561 atgaaaaatc gataaacctc aaattagcca ttaaggtact tattcataat aagaaacaga
     1621 tccaatccat gggcgccttc acttctaatg atattg
//
LOCUS       DAOQVW010000392         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_99193_length_1651_cov_3.747494, whole genome shotgun sequence.
ACCESSION   DAOQVW010000392 DAOQVW010000000
VERSION     DAOQVW010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99193_length_1651_cov_3.747494"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKK85_07825"
     CDS             complement(<1..556)
                     /locus_tag="PKK85_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HNS25964.1"
                     /translation="MIEVNDMFYAKSSDAAIADAGEYGGAVTSILKFLLKEGIVDAVL
                     AVEEGADLYDAVPILIEDPDKVVKSAGSIHFGTLNLAKVVEKYLDGAQDMKIAVTVKP
                     CDAMTMVELIKRERVNPDNVIMIGLNCGGTMPPVKSREMMEKFYEFDPDSVVKEEIAK
                     GKLIVETADGTEKEIPIDDLEDEGF"
     gene            complement(553..>1651)
                     /locus_tag="PKK85_07830"
     CDS             complement(553..>1651)
                     /locus_tag="PKK85_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048072979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNS25965.1"
                     /translation="GVNPLRGQNNVQGACDMGALPTDYPGYRKVMDEEVTEDISCTWG
                     CSDLGCEPGLKIPEMIEAAYKGDLKMLYITGEDPVISDPDVNHVIKALENLDFFVVQD
                     IFMTDTAEYADVVLPAACWAEQEGTFTNGERRVQLIRKAVDAPGDSKVDWEIFCDLAK
                     RMGADPEQFTYESAQDIFEEVRTVTPQYAGMNRERLEKPEALHWPCPSEDHPGTSMMH
                     VEQFSHPDGLGIFAALEEKGPMETPDDEYPLILTTTRILFHYHAAMTRRAETLDREVP
                     TGYMEINTEDAAELGIINDEIVKVKSRRGEIEIPARVTTDIMKGIVNIPMHFRECSAN
                     VLTNAAAIDPRSGMPEYKACAVAVSKMEGGK"
BASE COUNT          347 a          396 c          372 g          536 t
ORIGIN      
        1 cgaatccttc gtcttccagg tcgtcgatgg gtatttcttt ttcggtacca tcggcagttt
       61 caacaatgag tttacccttg gcgatttctt ccttgaccac gctgtctgga tcaaattcat
      121 agaatttttc catcatctct cggcttttaa cgggtggcat ggttccacca cagttgagac
      181 cgatcatgat cacgttgtct gggttaactc tttccctctt tattagttca accatggtca
      241 ttgcatcaca gggtttaact gtgactgcaa ttttcatgtc ctgggcacca tcaaggtact
      301 tttccacaac tttagctaag ttgagggttc caaagtgaat ggagccagca gactttacta
      361 ctttatcagg gtcttcgatt aggattggaa ctgcatcata aaggtctgcg ccttcttcga
      421 cggctagaac agcatctacg attccttcct tgagaaggaa tttcagaatc gaggtaactg
      481 ccccgccgta ttctcctgca tcagcgattg ctgcatctga agacttggca tagaacatgt
      541 cgttaacttc gatcatttac ctccctccat tttagatacg gctacagcac aggctttgta
      601 ttctggcatt ccagatctgg ggtctattgc agcggcgttg gtcaacacgt ttgctgaaca
      661 ctctctaaag tgcataggaa tgtttactat tcctttcatg atgtctgtgg tgactctagc
      721 tggaatttca atttcgcctc ttctggattt cactttgact atttcgtcgt tgataattcc
      781 gagttcagca gcgtcttcag tgtttatttc catgtaacca gttggtacct ctcgatccag
      841 tgtttcggct ctcctggtca tggcagcgtg gtagtggaac agaatacggg tggtggtgag
      901 tattagtgga tattcgtcgt ctggggtttc cattggtccc ttttcttcaa gtgctgcgaa
      961 tattcccagt ccatcaggat gtgaaaactg ttcaacgtgc atcatggaag ttccggggtg
     1021 atcttcagat gggcatggcc agtgtagtgc ttctggtttt tccagtcttt ccctgttcat
     1081 acctgcatat tgaggagtta cagttctgac ttcttcgaat atgtcctggg cagattcgta
     1141 ggtgaactgt tcaggatcgg cacccatcct cttggctagg tcacagaaga tttcccagtc
     1201 tactttggaa tcgcctggtg cgtccacggc ttttctgatt aactggactc tacgttcacc
     1261 attggtgaat gttccttctt gctcagccca gcaggcagca ggtaatacta catctgcata
     1321 ttcagcagtg tctgtcatga agatgtcttg caccacaaag aagtccaggt tttccagggc
     1381 ctttatcaca tggttgacgt ctgggtcaga aattacagga tcttccccgg tgatgtacaa
     1441 cattttaagg tcgcctttgt aagctgcttc tatcatttct gggattttaa gtccaggttc
     1501 gcatccaagg tcgctgcatc cccatgtgca ggatatatct tctgttactt cttcgtccat
     1561 aactttccgg tatcctggat aatcagttgg cagtgctccc atgtcacagg caccttgcac
     1621 gttgttctgt ccgcgcaatg ggttcacacc g
//
LOCUS       DAOQVW010000393         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_99418_length_1649_cov_2.860728, whole genome shotgun sequence.
ACCESSION   DAOQVW010000393 DAOQVW010000000
VERSION     DAOQVW010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99418_length_1649_cov_2.860728"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKK85_07835"
     CDS             complement(<1..457)
                     /locus_tag="PKK85_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNS25966.1"
                     /translation="MMGELAHEALHKAQKVVNEAEIYVESEKSLDIDIQKDKVGFAKK
                     VYSLGIGVRVITQGRMGFAYTTNLELMDETIQKAVDSARANLPDENFKFASFCSYPEV
                     QGLYDKRAEYSDVEEAVDFGKLLVATVHEEGCQPTSGGYALGIKESVLLN"
     gene            complement(454..1116)
                     /locus_tag="PKK85_07840"
     CDS             complement(454..1116)
                     /locus_tag="PKK85_07840"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNS25967.1"
                     /translation="MKALALDVDGTITDQKRRLCPGALKAIRKVEDRGIPVIIVTGNI
                     LCATKTISILLGTTGGLVAENGGVIEYEGEISILGNLEKCEKAFQHLRNLYDVRKVEY
                     SSARVSEIALERNLPAKKVKEALKNWDVEVYDTKFALHLTDPEVNKGLSLLNIAKKMR
                     VKPEEIMAIGDSENDLELLERAGWKVAVANSDQELKNKADYVTKRYYGDGVKEAIGRF
                     LL"
     gene            complement(1135..>1649)
                     /locus_tag="PKK85_07845"
     CDS             complement(1135..>1649)
                     /locus_tag="PKK85_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNS25968.1"
                     /translation="GHEAVGEIAERNLTAKGRKMVLDILGAEPLSGVATFPDSVRDDD
                     RFDDFSAYHFFNAPPGTSVKDAEKLLEEVFYIKTREWRGFPDIPNSVYELRDEFSQYN
                     ARQKFDLHVEQSTELATGCICGPILRGVARPEDCELFKEECTPTNPVGACMVSKEGTC
                     NIAYRYGSFV"
BASE COUNT          389 a          431 c          336 g          493 t
ORIGIN      
        1 aatttaacaa cacactctct ttaatgccca atgcataacc acctgaggtg ggctgacatc
       61 cctcttcatg gaccgtggct actagaagtt taccgaaatc aacagcctct tccacatctg
      121 aatattcagc ccgcttatca tacaggcctt ggacttcagg atagctgcaa aaagaggcga
      181 atttgaagtt ttcatcaggg agattagccc gggcactgtc tactgctttc tggatcgttt
      241 catccatcaa ctccaggttg gtggtgtagg caaagcccat ccttccctgg gtaataaccc
      301 ttacaccgat ccccagggag tacactttct tggcaaaccc cactttatcc ttttgtatgt
      361 caatatccag gcttttttca ctttccacat agatttcagc ctcattcaca actttctggg
      421 ccttgtgtag ggcttcatgc gctaattctc ccatcatagt aagaacctcc caattgcttc
      481 cttaaccccg tctccgtagt atcttttagt gacgtaatca gctttattct taagttcctg
      541 gtcggagtta gccactgcta ccttccagcc ggctcgttct aaaagttcca ggtcattttc
      601 gctatcacca atggccatga tctcttcagg tttcaccctc attttctttg caatatttag
      661 gagtgataat cctttgttca cttctggatc agttagatga agggcgaatt tggtgtcgta
      721 cacctccaca tcccaatttt taagggcttc cttcactttc tttgcgggta agttacgctc
      781 cagggctatt tccgaaacac gggctgatga gtactccact tttctaacgt cgtatagatt
      841 tcggagatgt tgaaatgctt tttcacattt ttccagatta cccagtatcg atatttcgcc
      901 ttcatactca attactcccc cattttcagc caccaagccc cctgtagttc ccagtaggat
      961 ggatattgtt ttagtggcgc agagtatgtt tccagtaacg attatgaccg gaatcccccg
     1021 gtcttcaacc tttcttatgg ctttcaaggc cccgggacac aatcgacgtt tctggtcggt
     1081 aatggtgcca tctacgtcca aggccaaggc tttaatagaa atactatcac cttcttatac
     1141 aaaagaacca taacggtaag ctatattgca ggtaccttct ttactgacca tacaagcccc
     1201 tacgggattg gtaggtgtgc attcctcctt gaataattca caatcctcag gacgggccac
     1261 tcctcgaagt atggggccgc atatacatcc tgtagcaagt tccgtgcttt gttccacatg
     1321 gagatcgaat ttttggcggg cattgtactg ggagaattca tcccgtagct cgtaaactga
     1381 attaggtata tctgggaacc ccctccactc cctggttttg atgtagaaaa cttcttccaa
     1441 aagtttttca gcgtctttga cggaagttcc gggcggcgca ttgaagaagt ggtaggccga
     1501 aaaatcgtcg aaacgatcgt cgtctcgcac ggaatcgggg aaggttgcta ctcctgaaag
     1561 tggctcggcg cccaagatat cgagcaccat cttcctacct tttgcagtga gattacgttc
     1621 ggcgatctca ccgaccgctt catggccga
//
LOCUS       DAOQVW010000394         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_100049_length_1643_cov_3.071788, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000394 DAOQVW010000000
VERSION     DAOQVW010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100049_length_1643_cov_3.071788"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /gene="cobA"
                     /locus_tag="PKK85_07850"
     CDS             complement(<1..470)
                     /gene="cobA"
                     /locus_tag="PKK85_07850"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNS25969.1"
                     /translation="MVVYLVGAGPGDPELVTIKAIKVLKKADVVIYDRLANEKILEEA
                     AGAKLIYVGKKAGVHHKTQDEINQILIHEGQKYDTVVRLKGGDPFVFGRGGEEMLALL
                     ENGIEVEVVPGVTSAIGVPGTIGLPVTHRGVATSFTVVTGHEDPTKAESQVKWV"
     gene            complement(532..1182)
                     /gene="purQ"
                     /locus_tag="PKK85_07855"
     CDS             complement(532..1182)
                     /gene="purQ"
                     /locus_tag="PKK85_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNS25970.1"
                     /translation="MKVGIIRFPGSNCDRDVFHVLGLVGCEGEYIWWNQRDLSKFDAI
                     VIPGGFSYGDYLRAGAIAAITPVIDGIKEVVKEDKPVLGICNGAQILAEVGLVPGVFT
                     LNENAQFICKWEKMEVKTTRSPFTRLYKKGETIKMPIAHGEGRYYNPHIDDLKDQDQV
                     VLSFVGNNPNGSIEGITGVCDLDGRVCAVMPHPERASESILGSDDGIKFFKGMVNY"
     gene            complement(1185..1439)
                     /gene="purS"
                     /locus_tag="PKK85_07860"
     CDS             complement(1185..1439)
                     /gene="purS"
                     /locus_tag="PKK85_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNS25971.1"
                     /translation="MKYQAEVRISLKKGMLNPEASTIQRALALLNYHVEDTKTAETVT
                     FSLEAKSPEDAREEVDQMCQRLLCNPIIHDYHITVIPGDE"
BASE COUNT          424 a          413 c          309 g          497 t
ORIGIN      
        1 aagacccatt tgacttgact ttcagctttg gtagggtcct catggccagt caccacagta
       61 aaggatgtgg caacgccgcg atgggttaca ggcaatccta tcgttccagg aactccaatg
      121 gcagaggtga ctccaggcac gacctccact tctattccat tctctagaag ggctaacatt
      181 tcctctccac cccttccaaa gacgaaggga tctcctccct tgagacgcac cacagtatca
      241 tatttttgac cctcatggat cagaatttgg tttatttcat cctgggtttt atgatgaact
      301 cctgcttttt taccaacgta gattagtttg gcacctgcag cttcttcaag tatcttctcg
      361 ttggccagcc ggtcatagat cactacatca gcttttttta atacttttat agctttaatt
      421 gtaaccaatt cagggtcacc tggacctgca cctactaaat aaacgaccat caattcacct
      481 tgttaaatga atatttttat agaccaatga tattatgatg cgccatttag gttaataatt
      541 aaccatgccc ttaaagaact ttataccatc atctgatcca agaattgact ctgaagcccg
      601 ctcaggatgg ggcataactg cacatactct tccatcaagg tcacaaactc cagttatgcc
      661 ttctatagat ccattaggat tgtttcccac gaagctgagc accacttgat cctgatcctt
      721 aagatcatca atgtggggat tatagtaacg cccctcacca tgggctattg gcatctttat
      781 agtttcccct ttcttataga gccttgtgaa gggacttcgg gtagttttaa cttccatctt
      841 ttcccacttg cagatgaact gggcattttc attgagggtg aagacaccag gaaccaaacc
      901 cacctcagcc aagatctgag caccattgca gatacctagc accggcttat cttccttaac
      961 tacctcttta attccatcga taactggtgt gatggcggct atggctccgg cacggaggta
     1021 gtctccgtag gagaacccgc caggtatcac tatggcgtcg aatttagata gatctctctg
     1081 gttccaccag atatattcac cttcacatcc caccagtccc aggacatgaa aaacgtcgcg
     1141 gtcacagtta gaaccaggga agcggattat acccaccttc atggttactc atctccaggg
     1201 attactgtga tatgataatc gtgtatgatg gggttgcaaa gaagtcgctg gcacatctgg
     1261 tcaacttctt cccgggcgtc ctctggactt tttgcttcca gagaaaatgt aacagtctct
     1321 gcagtcttgg tgtcttctac atggtaattt aaaagggcta gagccctctg gatggtagat
     1381 gcttccgggt tcaacattcc cttcttgaga cttatcctta cttcggcttg gtacttcatc
     1441 taaatcacaa tccattcata ttttaaaagc ggtgggtcta atatttatta ggctcacata
     1501 ttattattag tcaatttttt ccaactcaga ttccatcttt tcagatcatc ttcatccaga
     1561 attatctcag caacacgccg ataagcttca ataactcctg attctccttt tcgaaagaga
     1621 tccttgtcta aaacgtcacg cgt
//
LOCUS       DAOQVW010000395         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_100704_length_1637_cov_2.773704, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000395 DAOQVW010000000
VERSION     DAOQVW010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100704_length_1637_cov_2.773704"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PKK85_07865"
     CDS             <1..738
                     /locus_tag="PKK85_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNS25972.1"
                     /translation="DAPAPAYWIVTPDSSQEKVMLFFHGGLFSMGSTHSHQYLCQQLS
                     QITGRSIFSVDYHLSPEHPFPRALEDGINSYHWLVEEGFPARDIVCTGISAGGNLALS
                     TVLALKRMDENLPQAVVALSPLLDLSFPVVYQHLKDVKDWIDYHALHKLLDTYLQGQD
                     PGNFLLSPVYGDLEGLPPIFLQAGSCELLLCDVKRFRDQAMEQEVKVNMEVWQDMFHA
                     WQMFPTILAEAEQSLESVGKFVQDLVD"
     gene            832..1623
                     /locus_tag="PKK85_07870"
     CDS             832..1623
                     /locus_tag="PKK85_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNS25973.1"
                     /translation="MIKHKIVGNAMQMVVCELSHGEEVYCDAGKFLWKTSNMEMDTRF
                     GTSKKTQEKKGFLDQAIGTAIDIGKRSLAGESLAFVYFTPQGGDGLVAFAQMIPGEIK
                     TFELDGTQDLYVQSDGLLAAESSLEFNIALTKKLGAGFFGGQGFILQKYSDKGSLFIG
                     SCGNFIELNPADYGGTIHVDTGCLVAFEDTIDYDIEFVGGLDSRGIKNLLFGGEGIFF
                     ARLSGNGKVWVQSMNLSSLARTIVKSAGQPTAENRTDLGGILSGF"
BASE COUNT          407 a          427 c          457 g          346 t
ORIGIN      
        1 gatgcacctg caccggccta ctggatcgtt accccggata gcagccagga gaaggtaatg
       61 ctcttcttcc atgggggatt gttcagcatg ggctccaccc actcccacca gtacctgtgc
      121 cagcagctat cccagatcac cggcagatcc atattcagcg tggactacca cctctccccg
      181 gagcacccct tccccagggc cctggaggat ggtatcaact cctaccattg gctggtggag
      241 gagggattcc ccgcccggga tatagtgtgc accggtatat cagcaggggg gaacctggcc
      301 ctgtccacag tcctggccct gaaaaggatg gatgaaaacc tgccccaggc tgtggtggca
      361 ctgtcacccc tactggatct gagcttccct gtggtttacc agcacctgaa ggatgtgaag
      421 gactggatag attaccatgc tttgcataaa ctattggaca cctacctgca gggccaggat
      481 cccgggaact tcctactctc cccggtctac ggtgacctgg aggggttgcc acccatcttc
      541 ttacaggctg gcagctgcga actcctcctc tgtgatgtga aacgcttccg ggatcaggcc
      601 atggaacagg aggttaaggt gaacatggag gtctggcagg acatgttcca tgcctggcaa
      661 atgttcccca ccatcctagc cgaggcggaa cagtccctgg agagtgttgg aaaatttgtg
      721 caggacctgg tggattgaac tctttcaagc tgatccacag ataagcctaa ataccagggg
      781 ggatgttaaa ttcaactgaa atattgttat ctatagtaat ggtgattatg gatgattaaa
      841 cacaagatag taggaaacgc catgcagatg gtggtgtgcg aactctccca tggggaggag
      901 gtgtactgtg acgctggaaa gttcctgtgg aaaaccagta acatggaaat ggacacccga
      961 ttcggaacca gtaagaagac ccaggagaag aagggcttcc tggatcaggc cataggcaca
     1021 gccatagaca ttggaaaaag aagcctggcc ggggagagcc tggccttcgt gtacttcacc
     1081 ccccagggtg gggacggctt ggtggccttt gcccagatga taccaggcga gatcaagacc
     1141 ttcgaactgg acgggaccca ggatttatac gtccagtccg acggcctcct ggcggcagag
     1201 agcagcctgg aattcaacat agccctgaca aaaaaattag gagcaggctt cttcggggga
     1261 cagggattca tcctccagaa atacagtgac aagggctccc tcttcatagg atcctgtgga
     1321 aacttcatag aactcaaccc cgcagactac ggtggcacca tacacgtgga caccggttgc
     1381 ctggtggcat ttgaagacac catcgactat gacatagaat tcgttggggg cctggattcc
     1441 cggggaataa aaaatttact ctttggtggg gagggaatat tcttcgcccg cctctcaggc
     1501 aatggtaagg tatgggtgca gtccatgaac ctcagctcac tggcccggac catagttaaa
     1561 agtgctggcc agcccaccgc cgagaaccgc accgacctgg gcggaatttt gagcggattt
     1621 taggaggata aaacatg
//
LOCUS       DAOQVW010000396         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_100814_length_1636_cov_2.884250, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000396 DAOQVW010000000
VERSION     DAOQVW010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100814_length_1636_cov_2.884250"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..778
                     /locus_tag="PKK85_07875"
     CDS             98..778
                     /locus_tag="PKK85_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS25974.1"
                     /translation="MVLWGCSILFKEVLKLKDVNKSYPSSAHEEILVLNQISLKLSSK
                     GIYLILGPHGSGKSTLLRISGFLESPSSGSVAFAGRDLTEPESQERLNLIRNEIGFVP
                     PYPSLLPYLTILENVMLPMVRKNKFKVLEILKSIGVENNGSYPHQISVEEQQRASIAR
                     AMVNDPHILLVDEPTSPLSETGANEIMHILRDLKKEYTIMIFTDDPLLSKYSDTVFKL
                     HHGVLEKI"
     gene            1177..>1636
                     /locus_tag="PKK85_07880"
     CDS             1177..>1636
                     /locus_tag="PKK85_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25975.1"
                     /translation="MSFLNPEDNEMSPLQRNLLRILRMFLLLAGIYITLFGGANGAER
                     IFGILILVALAIINAPAFFTKGKIRIIPVEIELILLSMVLFELVGGDAMGLYVKLPHY
                     DNFMHFMLPLYIGLIGMMVVYTMHYYGRLKMSLAEMAIVIVIITIGLGGIL"
BASE COUNT          484 a          288 c          322 g          542 t
ORIGIN      
        1 gtggtgttac cgggatggtt gatcctcatg actctcacta caaccactgg ccttagtttc
       61 tttttgagtt tattccctgc atggttagca tctaaaaatg gttttgtggg ggtgttcaat
      121 cctgtttaag gaagtcctta aattaaagga tgtgaataag tcatatcctt cctctgcaca
      181 tgaagaaatc ctggttttaa accaaatatc cctcaaatta agttctaaag gaatatactt
      241 aattttaggc cctcatggtt cgggtaagtc tactctatta cgtatctccg gatttttaga
      301 aagtcctagt tcgggttcag tagcctttgc tggcagggat ttgacagaac ctgaatcaca
      361 ggaacgttta aatttaatcc gtaatgaaat aggttttgtt ccaccttatc cgagcctttt
      421 accttattta acgattttag aaaatgtaat gctgcctatg gtccgaaaaa acaaatttaa
      481 ggtgttggaa atactgaaaa gtataggtgt agaaaataat ggatcctatc cacaccaaat
      541 atcggttgaa gagcagcaaa gagccagcat agctcgtgct atggttaatg acccccatat
      601 tcttttggtt gatgaaccca cctctccatt atctgaaact ggagcaaatg agattatgca
      661 cattctgagg gatctaaaaa aggagtatac cattatgatt ttcaccgatg atcccctgct
      721 ttctaaatat tcagatacag tttttaagtt gcaccatgga gttttagaaa aaatttaata
      781 aaataagtcc ttatggatga agtattaatt ttttaaaaga tcattggact cattcttcta
      841 tttttttcat ccatataata tgtggtagag taccgatgca attcccactg ttaatggccc
      901 gataaataat cctggaagac tcaatgtaat aacttcaaac aggaaaccta ctaagaattc
      961 caggggatgt acttcgtgta ctttccagcc agcaaggggg atggatggta gtttttaggt
     1021 tagaaatcat ttcgaactcc gtctccctgg ttttattata agacccgatg tgaaaccatg
     1081 atctatatgc atacttcaat atgaatttat aaatgttagg attattatat ctacttaata
     1141 tgtctcatat tatgaaaaaa tagcgggaga ctatagatga gctttcttaa ccctgaagat
     1201 aatgaaatga gtcctctaca acggaattta ctccggatat tgcgaatgtt tttgcttctg
     1261 gctggtatct atattaccct ctttggcggt gccaatggtg cggaaaggat ttttgggata
     1321 ctcatactgg tagcattagc aattataaat gctcctgcat tttttacaaa aggtaaaata
     1381 cgtattatac ctgttgaaat tgaacttata ctgctgtcta tggtgttatt tgaactggtg
     1441 gggggagatg ccatgggttt atatgttaaa ctgccccatt acgataactt catgcacttc
     1501 atgctgccct tatatattgg cctgatcggt atgatggtgg tttacaccat gcactactac
     1561 ggccgtttaa agatgtcatt agcagagatg gccattgtga ttgttataat aaccatagga
     1621 ttaggtggta tacttg
//
LOCUS       DAOQVW010000397         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_101496_length_1630_cov_2.417778, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000397 DAOQVW010000000
VERSION     DAOQVW010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101496_length_1630_cov_2.417778"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            617..>1630
                     /locus_tag="PKK85_07885"
     CDS             617..>1630
                     /locus_tag="PKK85_07885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNS25976.1"
                     /translation="MLVALVIYLLTSLVVFRYYQHQINPDGVVYISVAKSYLCDNWIQ
                     AIDSYHSPLLSWLLVPFLFFDSTPTVALFFTKILSVIVGFFTIIGFWFLYSRFGLNKN
                     LKTVLTVVMVPLTIYFAFSVITPDLLMVCSLIYYLYFLFSPEYSNKIFNGIFCGLFAA
                     LAYLSKSFALPFFLIHFFLINALYYFNDPSRRENTVKNLLLGILVFFLISGVWIGLIS
                     DKEGQFTYGTAGKFNHDLVGPNSKGWGLPQVGGEFSEVESWSPFESWANFMHQLQIIY
                     SNLIQIFSILEYFSYFSILILLSYIILCIRPLKELIKLPKILIPLLTIIIFCGGYTPV
                     LVEE"
BASE COUNT          467 a          271 c          297 g          595 t
ORIGIN      
        1 gctgtgtatc tatgaatagt ttaaagaaaa taaatttgat ggtataaata cgaataaacg
       61 gatgaaacat atgtacaatt catttgatac gtttttctgt cggtggaagc tattttaaca
      121 atatctttat ttacgttcac agaatatttt gatgcatgat ctaatttcag ctgtaattca
      181 taggattaat tgccaactat cctctgaata ttaatgggtt cttcaataaa cgtactgtgg
      241 gcaacattgg ccaggattta cgaattttgc agccattagt atcatcggct ggggataaaa
      301 tgaactgata atctggtagt tcacgtatta cgtgggttaa gataatctca gcgctataat
      361 tctgtactgg aatttctttg cgagaagaat ttactttatt cagtagaagg tataattttt
      421 tggataaagg gaagtacaat atggccaatt ttttatagga acttacgctg tataaattta
      481 gacaaattga agtaaaggat atggtgttgg tagctactac aatggctgtg ccaggtttaa
      541 tattaaacta taattaaagg gtaggaatat ggggcagaat attcgctcac aaatagtaac
      601 ttggagttat ggtcctttgt tagttgcatt ggtcatttat ctactgacca gcctagtggt
      661 tttcagatat taccagcacc agataaatcc agatggtgtg gtgtatatca gtgttgcaaa
      721 gtcttatcta tgtgataact ggatccaggc cattgattct tatcacagcc ccctactatc
      781 atggttacta gtccctttcc tgttttttga ctctactcct actgttgctc tttttttcac
      841 taaaatactg tcggtgatag tgggattttt tactatcatt ggattttggt ttctatactc
      901 tcgttttgga cttaataaaa accttaaaac agttttaaca gttgtgatgg taccattaac
      961 aatctatttc gcattcagtg tcattactcc agatttactc atggtatgta gcttgattta
     1021 ttatttatat tttttattca gcccggaata ttctaataag atatttaacg gaatattttg
     1081 tggtttattc gctgctctgg cctatttgtc taaaagtttt gccttgccat ttttcctgat
     1141 ccatttcttt ttaattaatg ccctttatta ttttaatgat ccttcccgtc gtgaaaacac
     1201 cgttaaaaat ttacttttag gaattttagt atttttttta ataagtgggg tttggattgg
     1261 acttatcagt gataaagaag gccaatttac ttatggcacc gccgggaaat tcaaccacga
     1321 tctggtgggg ccaaactcta aaggatgggg gctgccccaa gtaggtggag aattttctga
     1381 agttgaatcc tggagtccct ttgaatcctg ggccaatttc atgcatcaac tgcaaataat
     1441 ctacagtaac ctaatccaga tctttagtat tttagaatat ttctcctatt tttccatctt
     1501 gatactgcta tcctacatca tactgtgcat acgtccttta aaggaattga taaaattacc
     1561 taaaatctta attccacttt taacaatcat aatattctgc ggtggctaca ctcccgtatt
     1621 agtggaggaa
//
LOCUS       DAOQVW010000398         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_102209_length_1623_cov_3.760204, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000398 DAOQVW010000000
VERSION     DAOQVW010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102209_length_1623_cov_3.760204"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKK85_07890"
     CDS             complement(<1..436)
                     /locus_tag="PKK85_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNS25977.1"
                     /translation="MELLTGEKTTTIRKLWKKPLNIGDRLHCYWNLVSKEREKLFEAE
                     VTGVEIMKFGDILQNEDLIREEGFEDASGLEVEFRKLYPDHTKEDSLFQVIKFRRLPR
                     EEWEGRKIDEKAMITKRADILFDLGKYQDSVMCYMAALRFDPQ"
     gene            complement(489..1184)
                     /locus_tag="PKK85_07895"
     CDS             complement(489..1184)
                     /locus_tag="PKK85_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNS25978.1"
                     /translation="MQLGGTVISSLEYPGKISLVIFTGGCNLRCPYCHNPQMISGGEE
                     VPREDIEGEISKESDYIDAVVITGGEPLLQCAEVKKLLEYSKKLGLLTKLDSNGGFPG
                     RLSGVINLVDYVALDVKAPFDDYNKIIGSDIGDKVKKSMEVCANSPVYLECRTTYVPG
                     LLNEKDVEKIAKDLNCDLYTLQQFRNRTVLDKKLQKTPNPSRNELFEIAKNIKPIFSN
                     LKVKTAEFGEEMV"
     gene            complement(1225..>1623)
                     /locus_tag="PKK85_07900"
     CDS             complement(1225..>1623)
                     /locus_tag="PKK85_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS25979.1"
                     /translation="LANEDIIQYMYRVKPVFSLTKLSYVAALATLKDHEYLERSRAAS
                     VESREHLYQGLKEFPMLKVFRSYANYILVDVRETGMNSAQLTEELMKMGVIVRNCSSF
                     RGLDDYWIRVSVGTIKEDDLFLKILKDILE"
BASE COUNT          421 a          382 c          298 g          522 t
ORIGIN      
        1 cttgggggtc aaaacggagt gcagccatgt aacacatcac ggagtcctgg tacttgccca
       61 gatcaaaaag tatgtcggct cttttggtta tcattgcctt ttcatcaatt tttcggcctt
      121 cccactcttc tcgtggcagg cgtcggaact ttatcacctg gaaaagagag tcttctttag
      181 tgtggtcggg gtaaagtttt ctgaattcaa cttccagtcc agatgcatct tcaaatcctt
      241 cctcccgaat taaatcttca ttttgcagga tatcaccgaa tttcattatt tcaactccgg
      301 ttacttccgc ttcaaaaagt ttttcacgct ccttagacac caaattccag taacaatgca
      361 gtcggtcccc tatattcagg ggcttcttcc ataactttcg tatggtggtg gttttttcac
      421 cagttaaaag ttccaagttg cggcttccaa aaagcagtat tggtatgtaa caccctccat
      481 gtttttattc aaaccatttc ttcgccaaat tctgcagttt tcacctttaa attactaaaa
      541 ataggtttta tatttttcgc aatctcaaac aattcattcc tggatggatt tggggttttt
      601 tggagttttt tatccaggac agtgcggttc cggaactgtt gcagggtgta aagatcgcaa
      661 tttagatctt tggctatttt ctccacatcc ttctcattaa gaaggcctgg cacgtaggtg
      721 gtgcggcact ccaaataaac aggtgaattg gcacatacct ccatgctttt tttaacttta
      781 tcgccgatat ctgaccctat gattttattg taatcatcaa atggggcctt aacatccagt
      841 gccacataat caaccagatt tattactcca gaaagtcttc ccggaaagcc accgttgcta
      901 tccaatttgg ttaaaagtcc cagttttttg gaatactcca gcagcttttt aacttctgca
      961 cactggagaa ggggttcacc accggtgata accacagcat ctatatagtc actctcttta
     1021 cttatttcac cctcaatatc ctctcgtggg acttcctctc ctccactaat catctggggg
     1081 ttgtgacagt agggacatct aaggttgcag cctcctgtga agataaccag agaaatcttt
     1141 cctgggtact ccagtgatga aattactgtg ccacctaact gcatattgat caattcacta
     1201 atcttgtttc attgaacacg tggtctattc taaaatatct tttaatatct ttagaaatag
     1261 atcgtcctct ttaattgttc caacgctaac cctgatccaa taatcatcca gtccacgaaa
     1321 tgaactacag tttctgacta taacacccat cttcatgagt tcttcagtta actgagccga
     1381 gttcataccg gtttctctca catccactag aatgtagtta gcgtaggatc tgaaaacctt
     1441 cagcatggga aattctttaa gaccctggta caagtgttcg cgactttcca ccgaagctgc
     1501 tcgtgagcgc tcaaggtatt cgtggtcctt taatgtggcc agggcagcaa cgtaagacaa
     1561 tttagtcaag ctgaaaactg gtttcactcg atacatgtac tggataatat cttcattggc
     1621 cag
//
LOCUS       DAOQVW010000399         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_102253_length_1623_cov_2.306122, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000399 DAOQVW010000000
VERSION     DAOQVW010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102253_length_1623_cov_2.306122"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKK85_07905"
     CDS             complement(<1..1076)
                     /locus_tag="PKK85_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS25980.1"
                     /translation="MDEIVRFSGVLRENGILASIRSTQTACDAASLVKGQGVDLRETL
                     ACIYLKDQRQRKKFDECYDVFFENKGKMADKPASNLEDKDKFIRKLNISVTKQNKASF
                     NYNDNFQYKMDYIKNTLNDADDNADHEGNSELITSNIHTLNTLQPELIDLCQKLGRKI
                     ATRRARRYKQSKKQKPDIRRTIRKNMKHGGSLLELVKSKPKIKKQNHFFLSDVSVSCD
                     WISIWFFCMIYAAQNSFNRARAFEFDNKTAEISTALQELNIVDAFIKVLNIRRMNSMI
                     QGKSNMYTAFQSFLNQANINHKSYVLILSDCRDWAGPKYEREPLSADFIGNMSRKAKR
                     VLILNPEPKIKWNAADSCVSYYEDA"
     gene            complement(1125..>1623)
                     /locus_tag="PKK85_07910"
     CDS             complement(1125..>1623)
                     /locus_tag="PKK85_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNS25981.1"
                     /translation="FINEKDSVILIDEIDKADEEVESFLLQALGEKQITVNDLGTFDL
                     KNDLLVIMTSNSQRQLLDETKDRCLYLYIDYPTFEREVAIVKSHSPEAPPSLIENVVK
                     VIQKIRKLNLIKTPSIRASVDWVSSLMIFGQNKLDEESLRKTINVVVKNEEDKNKVLK
                     NIKLD"
BASE COUNT          376 a          344 c          327 g          576 t
ORIGIN      
        1 gcatcttcat agtaggagac gcagctgtcg gctgcattcc attttatttt tggttccggg
       61 ttcaaaatca gtactctttt agcttttcta gacatattgc caataaaatc tgcacttaat
      121 ggttctcttt catatttagg cccggcccag tcacgacaat cacttaaaat caggacatat
      181 gatttatggt ttatattggc ctggttcagg aaactctgaa aagcagtgta catgtttgat
      241 tttccctgaa tcatggaatt cattctacgg atgtttaaaa ccttaataaa cgcatccacg
      301 atgtttagtt cttgtagtgc tgtggatatt tccgcggttt tgttgtcgaa ttcaaaggcc
      361 ctggcccggt tgaaggagtt ctgggcggcg tagatcatgc agaagaacca gatgctgatc
      421 cagtcacagg acacactcac atcactcagg aagaaatggt tctgcttttt tatctttggt
      481 ttacttttaa ccagttccag aagacttccg ccgtgtttca tatttttcct tatggtcctt
      541 ctgatatctg gcttctgttt ctttgattgt ttataccttc gcgcccttct ggtggcgatt
      601 tttcgtccga gcttctggca taaatcgatc aattcgggct gcagagtatt taaggtgtgg
      661 atgttgctcg tgatgagttc tgagttacct tcatggtctg cgttgtcgtc tgcatcgttc
      721 aacgtgtttt ttatgtagtc catcttgtac tggaagttgt cgttgtaatt gaaagaagct
      781 ttgttctgtt tggtgacgga gatgttcaat tttcttatga atttatcctt atcttccagg
      841 ttagaggcag gtttatctgc cattttcccc ttattttcaa agaatacgtc gtagcattca
      901 tcgaactttt ttctctgcct ctggtcttta aggtagatac aggctagggt ttcccttaaa
      961 tcaacaccct gtcccttaac cagggaagct gcatcacagg cggtttgtgt actcctgatg
     1021 cttgccagga ttccattctc ccttaacact ccggagaatc tgactatttc atccatagtt
     1081 ccacattcta aagaaagaat taatcttcaa tcgagttttc tggtttaatc gagtttaata
     1141 ttctttaaga ccttgttttt atcctcttca tttttaacca ccacgttgat ggtctttctt
     1201 aaggattcct catctaattt gttttggccg aatatcatca ggctactcac ccaatctaca
     1261 ctggcccgga tagaaggtgt tttaattaaa ttgagcttcc taattttctg tataaccttc
     1321 accacgtttt ctatcagtga tggaggagct tctggggagt gtgatttaac tatcgccact
     1381 tccctctcaa aggtggggta gtcgatgtaa agatacagac aacggtcttt ggtctcgtct
     1441 aagagctgtc tctgggaatt ggaggtcatg atgaccagta gatcattttt aagatcgaac
     1501 gtccccagat cattaactgt tatctgcttc tccccgaggg cttgaagcag gaaactttct
     1561 acttcctcat cagctttgtc gatttcatcg attaggatca ctgaatcctt ttcatttatg
     1621 aaa
//
LOCUS       DAOQVW010000400         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_102413_length_1621_cov_3.510856, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000400 DAOQVW010000000
VERSION     DAOQVW010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102413_length_1621_cov_3.510856"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..550
                     /locus_tag="PKK85_07915"
     CDS             200..550
                     /locus_tag="PKK85_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNS25982.1"
                     /translation="MISIKRAYDPADDEDGFRILVDRLWPRGVSKDRLKIDLWLKEVA
                     PSSQLRKWFAHDPQKWKEFQARYLEELKEKKEYLDQIEDIIRKEETVTLVYAAKDEDH
                     NNAQVLKELLSPRV"
     gene            complement(692..1579)
                     /locus_tag="PKK85_07920"
     CDS             complement(692..1579)
                     /locus_tag="PKK85_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS25983.1"
                     /translation="MKEIKVLLVEDNPGDALIIKEMLKEIYGAHFKVTHADRLVDGIK
                     HLKYTDFDILLLDLNLPDSQGIETIKGMNQKAPDLPIIILTGFSDEELAINSVGEGAQ
                     DYLVKGQIDSPLLSRSIKYSIERKNIERKLRKSEEKYRLMVEKIQSGVFLINSQNKLK
                     YVNKAMAKMLGYSVTEMIDRDIFNFTEPSEKNKLLDHLRSLKKKTAQTYELEFITNKG
                     SKACFLIATSALFKGKDYLGAISIMTDISARKGMEKSLMDAMIEKDQEFFFIMGSMVE
                     AMKPLIQQDKVENYHDRLT"
BASE COUNT          471 a          321 c          318 g          511 t
ORIGIN      
        1 ccaaaaaaat aggtttctgg catgaatatc agtttacagt aaaggtttgg ggggaatagt
       61 caactgttaa tctgcacccc tagtaaaact gtcccccttg aagttttttc atgtgattat
      121 cacctgatcg tcggatatac aatttgatgg acattttcat gcctctaatg gtgcagaaat
      181 ataaaatggg agtgttaaca tgataagtat caaacgtgcc tacgatcctg cagatgatga
      241 agatggcttc cgcattctag ttgatcgact gtggccccgt ggagtcagca aggacaggtt
      301 gaagatagat ttatggctta aggaagtggc tccatccagc cagcttcgta aatggttcgc
      361 acacgatccc caaaaatgga aagaattcca ggccagatac ctggaagaat tgaaggaaaa
      421 aaaggagtat ttagaccaga tagaagatat tatcagaaaa gaagaaactg taaccctggt
      481 ttacgctgct aaagatgagg atcataataa cgcccaggtt ctgaaggaac tcctgagtcc
      541 aagggtttga agcatggttt aaggtttgat ctgtggaaca aggagtaaat tactgccgat
      601 gggatttaat cattgaaaca tatttcacgg aatgatccct gtctgaattg tatgaattct
      661 cctcttccta ggtgcgacct gaattataaa tctaagtaag cctatcatgg tagttttcca
      721 ctttatcctg ttgaataagt ggtttcatgg cctccaccat actgcccata ataaagaaga
      781 actcctggtc cttctctatc atggcatcca ttaaggattt ttccattcct ttacgagcac
      841 taatatcggt catgatggaa atagcaccta aatagtcttt ccccttaaag agggcgctgg
      901 tggctattag aaaacatgcc ttagaacctt tattggtgat aaattcaagt tcataggtct
      961 gggctgtctt ttttttaaga cttctcaagt ggtccaataa tttgttcttt tcagatggct
     1021 ctgtgaagtt aaatatatcc ctatcaatca tctcagtcac actgtaccct aacattttgg
     1081 ccatggcttt gtttacgtat ttcagcttat tctgggaatt tattaaaaac actcctgatt
     1141 gtattttttc caccataagc cgatattttt cttcactttt tctcaacttt ctttctatgt
     1201 ttttccgctc tatggaatac ttgatggaac gagataggag tgggctgtca atctgtccct
     1261 taactaaata atcctgggca ccctccccta cggagttaat agctagttct tcatctgaaa
     1321 atccggtgag aatgataatg ggcaaatctg gagctttttg gttcatgcct ttaatggttt
     1381 caattccctg actatcagga agatttaaat ccaggagtaa aatatcaaaa tcagtgtact
     1441 taaggtgctt tatcccatca acaagtctat cggcgtgtgt tactttaaaa tgagcaccat
     1501 aaatttcctt aagcatttcc ttaattatca gagcgtcacc aggattgtct tccaccaaaa
     1561 gaaccttaat ttccttcatt tgatcaccgg ggaggtagtt taactatttc cagccagaaa
     1621 t
//
LOCUS       DAOQVW010000401         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_102469_length_1621_cov_2.114943, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000401 DAOQVW010000000
VERSION     DAOQVW010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102469_length_1621_cov_2.114943"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="PKK85_07925"
     CDS             complement(<1..1335)
                     /locus_tag="PKK85_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNS25984.1"
                     /translation="MKELNLNEELPVLVLPDRVLLHPTNMNMKIGKKIGSEIHQRVKD
                     HDFYGIVLAVKEFRAGRYAKHDFHRVGTLIRIENAHEMRDFYHLKVDILERVQIDELI
                     PDGMNYRAKYHLTPDEIDLDPVNQEEILKHIRFLVSEISENFKGAETFLEKVNQLDDL
                     NRAIAYVFPYMRLSVEERQKFLETRSLKEKSLSFLDMLIEQKESIQFQMEMAAKFNEE
                     MNKKHRENILKEQLKAIQDELSEVGGGPGRKDYRELIDESQMPDEVREVALEEVAKLD
                     RQGPNSSEENVIRNYLDLLVSLPWGESKVQDIDIEAARKVLDKEHYGLDKVKDRIIQH
                     MTVMKLKHNQQGSILLLVGPPGTGKTSLGKSIAEALGRKYVRISLGGVKDESEIRGHR
                     RTYLGALPGRIINGMKRAGEKNPVFILDEVDKLMASYSGDPASALLEVLDPEQ"
     gene            complement(1438..>1621)
                     /locus_tag="PKK85_07930"
     CDS             complement(1438..>1621)
                     /locus_tag="PKK85_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNS25985.1"
                     /translation="QPAASQHLKVLKTAKILVAQREGNYIYYTFNKQALQKHKNSIDF
                     LFNCALSKCEHLEKQE"
BASE COUNT          348 a          388 c          348 g          537 t
ORIGIN      
        1 ctgttccgga tccagaactt ccaggagggc gctggccggg tctccactgt aggaggccat
       61 taacttgtcc acttcatcca ggatgaagac cggatttttt tcaccggccc ttttcatacc
      121 attgatgatc cgaccaggca gagctcccag gtaggtgcgg cggtggccac gtatctctga
      181 ttcatcctta acaccaccta aactaattct aacgtatttt ctccccagtg cttcggcgat
      241 actttttcca aggctggttt tgccggttcc tgggggtccc accagtaaca ggatggatcc
      301 ctgctggtta tgtttcagtt tcataacagt catgtgctgg atgatcctat ccttcacttt
      361 atccaggccg tagtgctcct tatccagtac tttgcgggct gcctcaatgt ctatatcctg
      421 gactttactt tcaccccagg gtaggctgac cagaaggtcc aggtagtttc ggatgacatt
      481 ctcctcggaa ctgttgggac cctgcctgtc cagcttggcc acttcctcca gggccacctc
      541 ccgcacctca tctggcattt gggattcatc aataagttca cgataatctt tcctaccagg
      601 accaccccct acttcactga gttcgtcctg tatggccttc aactgctcct tgagtatatt
      661 ttcccggtgc ttcttattca tctcctcgtt gaatttggct gccatctcca tctggaattg
      721 gatagactcc ttttgttcaa tgagcatgtc caggaatgac agactttttt ccttcaagga
      781 gcgtgtttcc aggaattttt gtctttcttc cacagaaagt ctcatgtagg ggaacacata
      841 tgctattgcc cggtttagat cgtctagttg gtttaccttt tccaggaagg tttctgctcc
      901 tttaaagttt tcacttatct ctgagactaa aaaccggata tgcttcagga tttcctcctg
      961 gttcacaggg tccaggtcga tttcatcggg tgttaaatga tatttagccc gataattcat
     1021 gccatcaggg atgagttcat ctatctgaac tctttccaga atatccacct tcagatggta
     1081 gaagtccctc atttcatggg cattttcgat tctgatgagg gttcccaccc tatgaaagtc
     1141 atgtttagcg tatcggcccg cccggaattc cttcacggcc aggacaatgc cataaaaatc
     1201 atggtcctta accctttgat ggatttcgct cccgattttt ttgccaattt tcatgttcat
     1261 attggtgggg tgtaataaaa cacggtccgg taggaccagt accggtaatt cttcatttaa
     1321 attcagttct ttcatgttat cacgcaagat attggtttat aatgtgaaaa taggtatttt
     1381 ttttaatttt taaagtatta tttaaggatc tgccgttgct ggattgccct taagggtcta
     1441 ttcctgcttt tctaggtgtt cacatttgga caatgcacag ttaaataaaa aatcaattga
     1501 atttttgtgc ttctgcaggg cctgtttgtt gaaggtgtag tagatgtaat ttccttccct
     1561 ttgggccact aagatcttgg ctgttttgag gactttaagg tgttgtgatg cggctggctg
     1621 g
//
LOCUS       DAOQVW010000402         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_102886_length_1617_cov_1.616517, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000402 DAOQVW010000000
VERSION     DAOQVW010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102886_length_1617_cov_1.616517"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1103)
                     /locus_tag="PKK85_07935"
     CDS             complement(9..1103)
                     /locus_tag="PKK85_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS25986.1"
                     /translation="MAVDDRIKKIEEEIQKTPYNKATSHHIGKLKAKLSRLKEESLQK
                     SSSGAKGKGFHIKKSGDSTVVLVGFPSVGKSTILNQLTNAESKIGEYEFTTLEIIPGI
                     MEYKGAKIQIFDIPGIITGASKGKGRGKEILSVSRNADLIVVVLDVFNPQHMQVILDE
                     LRNIGIRPNESVPDVTVKKRKMGGINLSSTIPLTKIDDRTIRSILNEYGMHSADVLIR
                     DDVNIDQFIDSLDTNCRYIPMLLVVNKIDLADAEYLQKLKSELPDAIYFAADQGYHMD
                     LLREKIFQQLELIRIYLKPQGRKADLEEPLIVRKGSTVEEVALRLHRDFVRNFRQARV
                     WGSSVKFPGQKVGLEHHLQDQDVLRIIIKK"
     gene            complement(1257..>1617)
                     /locus_tag="PKK85_07940"
     CDS             complement(1257..>1617)
                     /locus_tag="PKK85_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HNS25987.1"
                     /translation="GNNVSAGIINVNELQSQNKSTQQLVNDLITGLQGNSTLIVKPHR
                     LTVMVNDTIVQNDIVNEDAIYSIALVNDKGTLMTYVYSKELEDSMFTKLYIFRGQNLT
                     QFNLAYEQPGVMVWNVV"
BASE COUNT          388 a          350 c          338 g          541 t
ORIGIN      
        1 tgttaaaatt atttcttaat aattatccgg agtacatcct ggtcctgcag gtggtgctcc
       61 agtcccactt tctggccggg gaacttcact gaggaccccc atacccgtgc ctggcgaaag
      121 ttccgtacga agtcacggtg taacctcaag gccacctcct ctaccgtgga cccctttctt
      181 acgatcaggg gttcttccag atccgcttta cgtccctggg gtttgaggta aatccttata
      241 agttccagtt gctggaatat tttctcccgt aggagatcca tgtggtaccc ctggtctgcg
      301 gcgaagtaga tggcatccgg tagttcagat tttaattttt gaaggtattc tgcatctgcc
      361 aggtctatct tgtttaccac cagtaacatg gggatgtacc ggcagttggt gtccagtgaa
      421 tcgatgaact ggtcgatgtt tacatcatcc cggatgagta catcggcact gtgcatcccg
      481 tattcattca ggatggatct tatggttcta tcatctattt tagtaagagg aatggttgat
      541 gataggttga ttccacccat cttcctcttt tttactgtga cgtccggtac tgattcgttg
      601 ggtcttatcc ctatattcct aagttcatca agtatcacct gcatgtgctg ggggttgaac
      661 acgtctaaga ccaccactat caaatctgca ttccgggata cggagagtat ctcttttccc
      721 cttcctttac cctttgaggc gccggtgatt atcccgggga tatcgaagat ctgtatttta
      781 gcccctttat actccatgat gcctgggatg atctccaggg tggtgaattc gtattcacca
      841 atctttgact cagcattggt aagctggttt agtatggtgg atttacccac ggaagggaac
      901 cctaccagaa ctacggtgga gtctccactt tttttaatat ggaaaccctt acctttagcc
      961 ccggaactgc tcttttgaag tgattcttcc ttcaatcgag atagtttagc tttaagcttc
     1021 cctatatgat gggatgttgc tttgttgtag ggggttttct ggatttcttc ctctatcttc
     1081 tttattctat catctacagc cataaaattc acataaaatt aaattcaatt ctagttttaa
     1141 agtagaatct atcttttatt tatgatatta attgtatttt aattgacata aaaaaattac
     1201 gagaataccc tctgaatttt aacaaaaaat gaaaaaggag aagtagtatt ggggttttat
     1261 actacgttcc agaccattac tcctggctgc tcataggcta aattgaactg ggtcaagttc
     1321 tgtcccctga atatgtatag tttggtgaac atggaatctt ccagctcctt gctatataca
     1381 taagtcatta aggttccttt gtcatttacc agggcgatgg agtaaattgc atcctcattt
     1441 acaatatcat tctggacaat ggtgtcgtta accatcacgg tcaacctgtg gggttttacg
     1501 ataagggtac tgttaccctg caacccagtt ataagatcgt ttacgagttg ttgggtgctt
     1561 ttattctgac tctgtaattc atttacgttg ataatacccg cagatacgtt gttgcca
//
LOCUS       DAOQVW010000403         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_103105_length_1614_cov_3.641437, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000403 DAOQVW010000000
VERSION     DAOQVW010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103105_length_1614_cov_3.641437"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKK85_07945"
     CDS             <1..473
                     /locus_tag="PKK85_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNS25988.1"
                     /translation="PTTTTTFTTITPPVVYSTPGTGSYWAPVSVVLAASEPATIYYTL
                     DGTSPTTRSARYSAPIYLAASRTLKFLGIDRAGNPSLISTQHYSIYALQSYRYWTYVR
                     VYGRYKYSYKVAIKRWYRKNGKWRYHYKYITKTKWKKGWHYVGAWKTGTRWVLT"
     gene            538..1209
                     /locus_tag="PKK85_07950"
     CDS             538..1209
                     /locus_tag="PKK85_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25989.1"
                     /translation="MKHSPEMELLVVVVMGLWGLIFLWLIIPQPVAMFNYLPLFLFSY
                     VLAMILPGYTFLRFTKPGMDFRTHLEMGLLLSLVFLLVVTSLFIFLEIPLAISYAVSV
                     LMILSLVMAILTYLGARMEMRKSPPEEKTSPHDPGADTTQRVREPVPEADEKTQGSLE
                     DPSLGRTRLTPPPGEVELIIPGHVHPGGGDGKIKARSTRETGEKSTLKLPWIERQKEL
                     DRKKK"
     gene            1268..1339
                     /locus_tag="PKK85_07955"
     tRNA            1268..1339
                     /locus_tag="PKK85_07955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Gln,seq:ctg)
     gene            complement(1358..1585)
                     /locus_tag="PKK85_07960"
     CDS             complement(1358..1585)
                     /locus_tag="PKK85_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25990.1"
                     /translation="MADFKYLKNQEKRIEKKKTELNDKTESLLTEARMDKIKEKYEDL
                     LKKKKSGLLTWSEEELLAIYQDAIKMEKEHK"
BASE COUNT          418 a          413 c          360 g          423 t
ORIGIN      
        1 gaccaaccac caccacaacc ttcaccacca ttaccccgcc agtggtatac tccactcctg
       61 ggactgggag ttactgggct ccggtttcag tggtgttggc agccagtgaa ccagccacca
      121 tctactacac cctggatggt actagtccca ctacacgtag tgcccggtac tctgctccca
      181 tctacctggc agcttccagg accctgaagt tcctgggtat tgacagggcc ggcaacccca
      241 gcctcatcag cacccaacac tacagcatct acgccctgca gagctacagg tactggacct
      301 atgtccgggt gtatggacgg tacaagtaca gctacaaggt tgctatcaaa cgttggtacc
      361 gtaaaaacgg taaatggaga taccactaca agtacatcac caagaccaaa tggaaaaagg
      421 gatggcacta tgtaggcgcc tggaaaacag gaacaagatg ggtcttaacc taaaggccct
      481 gccccatttt tttctcttca acttttatat ctgaaaagct acgaatagat tttcattatg
      541 aagcattccc cagagatgga gctcctggtg gtggtggtta tgggtctgtg gggactcatc
      601 ttcctgtggc tcatcatacc ccagccagtg gcaatgttca actacctgcc cctattcctg
      661 ttctcctatg tactagccat gattctccct ggttacacct ttctaagatt caccaagcca
      721 ggtatggatt tccgtacaca cctggagatg ggcctactcc tgagtctggt gttcctattg
      781 gtggttactt ccctgttcat cttccttgag atcccactgg ccatttccta tgcagtttca
      841 gttctgatga tactgagcct agtcatggcc atcctgacct acctgggggc caggatggag
      901 atgagaaagt cgccgcctga ggaaaagaca agcccccatg atccaggtgc agacaccaca
      961 caacgggttc gagagcctgt gcctgaagct gatgagaaga cccagggaag cttggaagat
     1021 ccctcactgg gaagaacccg cctcacacca cccccagggg aggtggagct tatcatcccc
     1081 ggccatgtgc accctggagg aggggatgga aagattaaag ccagatccac ccgggaaact
     1141 ggggagaagt ccaccctgaa gttgccctgg atcgagaggc agaaggagtt ggacaggaag
     1201 aaaaagtaga agcactggta atggatggta aaggtataat taccataaac taaaaataca
     1261 attttttagt cccttggggt agtggtaatc ctgtaaggct ctggaccttt cgacggcggt
     1321 tcgactccgc cagggactat aacatgaatt aaattgatta tttatgctct ttttccattt
     1381 ttatagcgtc ttggtaaata gctaacaact cttcctctga ccaggttaat aaaccagact
     1441 tcttcttttt aagcagatcc tcatattttt ctttaatctt atccattcta gcttcagtta
     1501 agagtgattc tgtcttatca tttaattcag tcttcttttt ctctatacgt ttttcctggt
     1561 tttttagata tttaaaatct gccatttacg ctcaccaaga ctaaataaag aaag
//
LOCUS       DAOQVW010000404         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_103427_length_1611_cov_4.404884, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000404 DAOQVW010000000
VERSION     DAOQVW010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103427_length_1611_cov_4.404884"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..874
                     /locus_tag="PKK85_07965"
     CDS             38..874
                     /locus_tag="PKK85_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNS25991.1"
                     /translation="MRPITHEKLPENPCKVDWNQLWKEQVEKLPKKKDLWNSIAPQFN
                     EWMDKDDYPAEFISKIKIEPNDTVLDVGCGNGAITIPLAQKARSVTALDSSTRMLEFL
                     GKKADHPSNIHMINKGLEEVDAQEIGYHDVVVASRSLNGIADIQAQLEKLDRIARKYV
                     YITLWGVDNRKFENEMAELLGRESHQNPEYILVYNILYEMGIQANVEFLKSNTRNHYS
                     NMDEALDRIKWRIGDLDEGEKSIIKKHLERVLTRNSDGSLTYTRTNSKWVLIWWEKSH
                     PS"
     gene            946..1530
                     /locus_tag="PKK85_07970"
     CDS             946..1530
                     /locus_tag="PKK85_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNS25992.1"
                     /translation="MDEIIAKIDDPEMRSQIDKLVPFHGYLSTGAFIGLQMLQIATRL
                     LDIKKGERIYVTSETYNCLPDPFQILHGSTIGNKGLKVLDYDKMAVTINKGAKPGQEY
                     VEGVRIFLDPAKTIKYPVFHAWYMNQRKVPHEEAISELLKAGEDVYTWEYVEVQVPVK
                     AKKDVAICQICGESFVKKDEEEICRGCAQEKNRK"
BASE COUNT          517 a          388 c          413 g          293 t
ORIGIN      
        1 cttatatgca cccacatcct agagatagaa ctggaaggtg aggcccatca cccatgaaaa
       61 attacctgaa aatccctgca aagtggactg gaaccagctg tggaaggaac aggtggaaaa
      121 actccctaaa aagaaggatc tgtggaacag catcgcccct caattcaacg agtggatgga
      181 taaagatgac tacccagccg agttcatcag caagataaaa atagaaccca acgacacggt
      241 gctggatgtg ggctgtggaa acggtgctat aaccattcca ttggcacaga aggccaggtc
      301 agtcactgcc ctggacagct ccaccagaat gctggaattt ttaggcaaaa aagcagacca
      361 cccctccaac atccacatga taaacaaggg cctggaggag gtggacgccc aggaaatagg
      421 ctaccacgat gtggtggtgg catcccggtc cctgaatggc atagcagaca tccaagccca
      481 gctggagaag ctagacagga tcgcccggaa atacgtttac atcaccctct ggggagtgga
      541 caaccgcaaa tttgaaaatg aaatggccga actcctggga agggaaagcc atcaaaaccc
      601 ggagtacatc ctggtctaca acatcctcta cgaaatggga atccaggcca acgtggaatt
      661 tttaaaatca aacacccgca accactactc caacatggac gaggccctgg acaggattaa
      721 atggaggatc ggggacctgg atgaagggga aaaatccata ataaagaagc acctggaaag
      781 ggtcctgacc agaaactccg atggaagcct cacctacacc cggaccaact ccaagtgggt
      841 tctcatctgg tgggagaagt cacacccctc atgataccat tacccactag gaggccaagg
      901 tccccccata tttattgaaa agctagaacc cgaggtggat tatgtatgga tgaaatcata
      961 gccaaaatag atgacccaga gatgaggtcc cagatagata aactagtacc cttccatgga
     1021 tacctgagca ccggggcctt cataggcttg cagatgctgc aaatcgccac acgcttactg
     1081 gatatcaaga agggagaacg gatatacgtc accagtgaaa cctacaactg cctacccgat
     1141 cccttccaga tactgcacgg gagcaccatt ggaaacaagg gattaaaggt cctggattat
     1201 gataaaatgg cggttacaat aaacaagggt gcaaaacctg gccaggaata cgtggagggt
     1261 gtgaggatat tcctggaccc agccaagacc atcaagtacc cggtgttcca cgcctggtac
     1321 atgaaccaga ggaaggtgcc ccatgaagaa gccatatcag agcttttaaa ggcaggagaa
     1381 gacgtgtaca cctgggagta tgtggaggtc caggtgccgg tgaaggccaa aaaagatgtg
     1441 gccatctgcc agatctgtgg ggagtccttt gtaaagaagg atgaagagga aatctgccgg
     1501 ggctgcgccc aggaaaaaaa ccgtaaataa cagatagccc ttaagatccc ccaggtagat
     1561 gctgataata gatgtccagg aaaaatgatc caggacctca gatattggag g
//
LOCUS       DAOQVW010000405         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_103722_length_1609_cov_2.362934, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000405 DAOQVW010000000
VERSION     DAOQVW010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103722_length_1609_cov_2.362934"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKK85_07975"
     CDS             complement(<1..294)
                     /locus_tag="PKK85_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNS25993.1"
                     /translation="MIMDGLLIMGVVISIYMAFNIAANDIGNSMGTVVGSGALKMRHA
                     LLLGAFFELVGALYFGSEVIKTVGSGIIAPDVLQATGAFIITLSAALWITITLL"
     gene            386..701
                     /gene="ffs"
                     /locus_tag="PKK85_07980"
     ncRNA           386..701
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKK85_07980"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            768..852
                     /locus_tag="PKK85_07985"
     tRNA            768..852
                     /locus_tag="PKK85_07985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:802..804,aa:Ser,seq:gct)
     gene            962..1462
                     /locus_tag="PKK85_07990"
     CDS             962..1462
                     /locus_tag="PKK85_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25994.1"
                     /translation="MALDDIVYVFLIIAIVGPIVQLFLSKAPRTKNRVFELFLVWFLF
                     LMVGVGSIWAFMGHVFDANAVAAVIGWPTGSPFQYEVGIANLAFGVLGLLCLKIRENF
                     WVATIIGFSIFYLGAAYGHIVNYMQTGNTAPGNIGFALYMDILVPIVLICLLIAYKRT
                     LKSVED"
BASE COUNT          424 a          379 c          364 g          442 t
ORIGIN      
        1 cagcagggtt atggttatcc acagagccgc agatagggtt atgatgaagg ccccggtggc
       61 ctgcaggaca tccggggcga tgataccact ccccaccgtc ttgataactt cactcccaaa
      121 gtacagtgcc cctaccaatt caaaaaacgc ccccagtaac agggcatgac gcattttaag
      181 ggcaccactg cctaccactg tccccattga gttaccaata tcattggcgg ctatgttaaa
      241 ggccatgtag atgctgatga ccacgcccat aatcaaaagc ccatccatga tcatttgtat
      301 gttctagact ggtgaaatat cattccaatc aatgaaggtt aagcaattga aacacaacct
      361 taaaatacca cctaccacca acactaaatg ggctagactg gagggttagg ggtcctctgt
      421 aagcgcacat cccctacacg gcgcagtcga agttcaagag gcggcagatt aactgtctct
      481 tggcccagag gatcaatgct gaaacctcgt cccacaggat cactggtggt tgggcactgg
      541 ctggagggcc ggtgttaacg cccttaacca ggggaacgtg tcaggtctgg aaagaagcag
      601 ctctactctg gtcagctgat gcttgtggaa cagcggggtg gagttggcct tctggatcac
      661 cattggacac gcggtctgtc cactcttgaa cacgcccact acattcacca ccaggatact
      721 tgtgtttaaa tagtagagga accaatctaa atacctacat tttatatgcc ggggtggccc
      781 agtctggtac ggcgttggcc tgctaagcca atgatccttt ggatctcgcg ggttcaaatc
      841 ccgtccccgg cgtttaacat actatttttc cattaattcc aggaccagca gataatttta
      901 tgtacagatt cgtacaaatc aattgttaac ctatgattca attatgggag gtgtatggta
      961 aatggcactg gatgatatag tatacgtgtt tttgataata gccatagttg gtccgatagt
     1021 gcagctattt ttaagtaaag caccccggac gaaaaacaga gtttttgaac tatttttagt
     1081 atggttttta ttcttgatgg ttggagtggg ctccatatgg gcctttatgg gccatgtttt
     1141 cgatgccaat gcggtggccg ctgtaatagg ctggcctaca ggcagcccct tccagtatga
     1201 agtgggtata gccaacctgg cctttggagt gttaggtttg ttgtgcctta agatcaggga
     1261 gaatttctgg gtagccacta taatcggatt ttcaatattc tacctgggag cagcatatgg
     1321 acatatcgtg aattacatgc agacgggcaa cacggcacca ggaaacatag gctttgcact
     1381 ctacatggac attctggttc ccatagtctt gatatgctta cttatagcct ataaaagaac
     1441 attaaaatca gttgaagact aaaactcccc ttcctttttt tctttatttc gtgtagctaa
     1501 tcctttttct aattctataa ctttacatcc cggaacatac caacgattag ataaaatcca
     1561 gctaaacctc tccatttccc ccatcgagtg gctattttcc gggcgttat
//
LOCUS       DAOQVW010000406         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_104323_length_1603_cov_3.017442, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000406 DAOQVW010000000
VERSION     DAOQVW010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104323_length_1603_cov_3.017442"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKK85_07995"
     CDS             complement(<1..340)
                     /locus_tag="PKK85_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS25995.1"
                     /translation="MEREAAELFEEFKELTASEFFRRNKQMLGFSGKIRSLTIVFHEL
                     ITNSLDAAEEAGILPEIKIDLKRLEKDHYILKHQDNGPGIPEHYITRVFSTMFAGSKF
                     LRIQRCAICHA"
     gene            complement(401..973)
                     /locus_tag="PKK85_08000"
     CDS             complement(401..973)
                     /locus_tag="PKK85_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNS25996.1"
                     /translation="MPTTEYLKIPQERVAVLIGSKGTTKGLIEKTTNTHLEIDSEAGS
                     VSISAGEENEDPLSVWKARYMIKAIGRGFNPEIALKLAGDEIMLDIINLPDYVGKSKK
                     ALTRQKSRIIGKDGRTRDIITEMAEVDVSIYGKTVSLIGDMEHLQVAHEAVEMILNGS
                     RHKTVYAFLEKKKQKRRMKEFQETHKIADL"
     gene            complement(1022..>1603)
                     /locus_tag="PKK85_08005"
     CDS             complement(1022..>1603)
                     /locus_tag="PKK85_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HNS25997.1"
                     /translation="VFKGVDDQGNLVAVKIYRVSTSDFKKMQYYIQGDPRFKVNTSNK
                     RQMIKTWVMKEFRNLKRAEEAGVPVPHPIIAKNNVLIMEFIGDSFGNPAPLMRETKIA
                     NPKKMVDEIVDIVVKLYNDAEIIHGDLSSFNILICDDKPVVIDVSQAVVRDHPLAMGL
                     LNRDIENLIRDFKKLGINISINEIKSKIMDVKG"
BASE COUNT          413 a          355 c          296 g          539 t
ORIGIN      
        1 atgcgtggca gatcgcgcag cgttgtatgc gaaggaattt ggaaccagcg aacatggtgc
       61 tgaaaacccg ggtgatgtaa tgctccggga tccctggtcc attatcctga tgtttaagga
      121 tgtagtgatc cttttctaaa cgtttgaggt caatttttat ttcaggaaga attcctgcct
      181 cttcagccgc gtccagactg ttagttataa gttcgtggaa tacaatggtt aaagaacgaa
      241 ttttacctga aaaacccagc atctgtttgt ttcgccggaa aaattcggat gcagtgagtt
      301 ctttaaactc ttcaaacaat tcagctgctt ctctctccaa attaagcctc cttaatagtg
      361 gtcatatatc ataatcaata gtaataatcc attaatggtt ctacaaatcc gctatcttgt
      421 gggtttcctg aaactccttc atccttcgtt tttgtttctt tttctccaga aaagcataaa
      481 ccgtcttgtg gcgggagcca ttaaggatca tttccacagc ttcatgagcc acttgaaggt
      541 gctccatatc cccaatgagg gaaacggttt ttccatatat ggaaacatct acttcggcca
      601 tttcagtaat tatatcccta gttcgtccgt ctttaccaat tattcgactt ttttgtcgtg
      661 ttaatgcctt tttcgacttt cccacatagt ctggtaggtt tattatgtcc agcataatct
      721 cgtcgccggc cagtttaagg gctatttcag ggttgaatcc tcgtccaatg gctttaatca
      781 tgtagcgtgc tttccacact gaaagaggat cttcattctc ttcgccggca gatatagaaa
      841 cagatccggc ttcgctatca atttccaggt gagtgttggt cgttttttca atcagcccct
      901 tggtggttcc cttgcttcca attaaaactg ccactctctc ttgggggatc ttcagatatt
      961 cggtagtggg caatctaaca cctcaatgaa aatattacat tatcctttaa gatatacttt
     1021 tctatccctt tacatccata attttacttt taatctcatt tatggatata tttattccta
     1081 atttcttaaa atctctaatt aaattctcaa tatcgcgatt taaaagcccc atggccaaag
     1141 gatgatcccg gactaccgcc tgtgaaacgt caataaccac cggcttatca tcgcagatga
     1201 ggatgttaaa gctcgataag tctccatgga tgatttctgc atcattatat agtttgacaa
     1261 caatatccac aatctcatcc accatttttt tgggattagc tatttttgtt tctctcataa
     1321 ggggggcggg gtttccgaaa gaatcaccta taaattccat aattaaaaca ttgtttttgg
     1381 caatgatggg gtgtggaacc gggacacctg cctcttcagc tcttttaaga ttcctgaatt
     1441 ctttcatcac ccacgtttta atcatttggc gtttattgga ggtgttaact ttgaaacggg
     1501 gatctccctg tatgtagtac tgcatttttt taaaatcaga ggtgcttacg cggtatatct
     1561 tcaccgccac caagttaccc tgatcatcaa cacctttgaa aac
//
LOCUS       DAOQVW010000407         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_104357_length_1603_cov_2.330749, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000407 DAOQVW010000000
VERSION     DAOQVW010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104357_length_1603_cov_2.330749"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PKK85_08010"
     CDS             complement(<1..270)
                     /locus_tag="PKK85_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS25998.1"
                     /translation="MKKIRKQAITLAITFLFVLIFAGAASAANVTVGPDSTYNYQAIQ
                     SAVDASADSDTITVYPNGNDVYNENVIVNKLLNIVANSSDTVVNGS"
     gene            complement(565..>1603)
                     /gene="dacB"
                     /locus_tag="PKK85_08015"
     CDS             complement(565..>1603)
                     /gene="dacB"
                     /locus_tag="PKK85_08015"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNS25999.1"
                     /translation="RVEGDVIIDDRLFQKTLAPSGEYLITPIMINDNLIDLEIVPGRA
                     GENATINCRPQSSTYTVTSQVQTVSSGQTEIRVNSDDPSKIVVEGTILENSTPQVRTV
                     SVEDPSSFARALFIEALKREGVEVTTPATNPNQHLASNNTTAYTEDKRVALFTSSPFS
                     ENIKVVLKVSQNMQADTLVALLAAKNGKKTFDEGMKLFGDFLTSAGVNTDMLALIDGR
                     GGSPSDRISPEASNQLLSYMARQSYYQSFFDALPVLGYDGTLSGSVTNSSPLYGKVNA
                     KAGTVTVDDKLNGRVILLSKSLDGYMVTRSGKKLIFSIYVNNVPLNNTNDANQVEQDI
                     NKVLEAIYYNL"
BASE COUNT          428 a          340 c          358 g          477 t
ORIGIN      
        1 actaccatta accacggtgt cagaactgtt ggccacaatg ttcagaagtt tgtttacaat
       61 tacattttca ttgtaaacat cgttaccatt aggatacacg gtaatggtat ctgaatccgc
      121 gctggcatcc actgccgatt ggatagcctg ataattatag gttgaatcag gccctaccgt
      181 cacattggca gcggaagctg ctccagcaaa tattagaacg aatagaaagg taatagctag
      241 tgtaattgct tgttttctta tctttttcac ctccttcatg ccggatataa gtcatgttga
      301 tgaacaccaa aaattcaaca ttgtttatgt tgaaaaatat tcatcattac catgaataat
      361 gtgaatttag aactagtcct tattaaatat tatcattatt taagatggta tgtaacatac
      421 aaaaaaggta caagaacaaa taaattgtgg attaaatact aataaacagt tatttgaaca
      481 aaattttaac aaataattat tttaaagacg ttttgtatat gaaatttatt attagaacta
      541 tttataacgg ttcttatcaa ctttttataa attgtaatat atggcctcta aaactttgtt
      601 tatatcctgc tctacctggt tcgcatcatt agtgttattt aagggcacgt tgttaacgta
      661 aatggagaat atgagttttt ttccactcct ggtgaccatg tagccatcca gggatttgct
      721 taacaggatc acacgaccat tgagtttatc atcgactgtg actgtgccgg ctttggcatt
      781 caccttccca tagagggggc tgctgttggt aactgacccg gatagggtcc catcatatcc
      841 tagaaccggt aaggcgtcga agaagctctg ataatagctc tgccgggcca tgtaacttaa
      901 aagctggttg ctggcctcgg ggctgatccg gtcagacgga gaacctcccc taccatctat
      961 taaggccagc atatcagtgt taacaccagc actagtcaga aaatcgccaa agagtttcat
     1021 accctcatca aaggttttct taccattctt ggccgctaaa agggccacca gggtgtcggc
     1081 ctgcatgttc tggctgacct tcaacaccac tttgatgttc tctgagaagg gtgaagaagt
     1141 gaataaagcc acccttttat cctcggtgta ggcggtggtg ttattggatg cgaggtgctg
     1201 gtttgggtta gtggccggtg tggtaacctc cactccctcc cttttaaggg cctcgatgaa
     1261 gagtgcccgg gcaaaggagg agggatcctc cacggataca gttcgcactt ggggggtgga
     1321 gttctccagg atggttccct ccaccacgat cttacttgga tcatcactgt tgaccctgat
     1381 ctcagtctgg cctgatgaaa cagtttggac ctgggaggtg acggtgtagg tgctgctctg
     1441 cggcctacag ttaatggtgg cattttcccc agcccttcca gggactattt ccaggtcgat
     1501 gaggttgtcg ttaatcatga taggagtgat taagtactcc cccgaggggg ccagggtttt
     1561 ttggaataac cggtcatcga tgatgacatc accctccacc ctg
//
LOCUS       DAOQVW010000408         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_104526_length_1601_cov_3.220569, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000408 DAOQVW010000000
VERSION     DAOQVW010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104526_length_1601_cov_3.220569"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /gene="rps2P"
                     /locus_tag="PKK85_08020"
     CDS             complement(<1..223)
                     /gene="rps2P"
                     /locus_tag="PKK85_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033290.1"
                     /note="one of the last subunits in the assembly of the 30S
                     subunit; absence of S2 does not inhibit assembly but
                     results in an inactive subunit; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNS26000.1"
                     /translation="MSELLIPLDKYLAAGLHIGTQQKTKDMEHYIYRVRTDGLYVLDV
                     RGTNDRIISAAKFLAKYDPSDILVVSTRQY"
     gene            complement(262..450)
                     /locus_tag="PKK85_08025"
     CDS             complement(262..450)
                     /locus_tag="PKK85_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS26001.1"
                     /translation="MVKVTIDQDKCEGADCAECVDVCPMEVLILDGDKVVVQNVEDCS
                     LCEVCMDVCPNEAVKVED"
     gene            complement(480..>1601)
                     /gene="eno"
                     /locus_tag="PKK85_08030"
     CDS             complement(480..>1601)
                     /gene="eno"
                     /locus_tag="PKK85_08030"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNS26002.1"
                     /translation="EVVAFPEGGVDKIIGELEDVISTELIGLDAEDLREIDLVLKEID
                     GTENLSSLGGNTTVAVSMATAKAAASSYNLPLYRFLGGNLPTSIPYPLGNMINGGAHA
                     GKNAPDIQEFLVLPVGAEDITEAVFTNTAVHRRIREKIQAKDKLFTGGKGDEGGWAPN
                     LSNQEALEIQASSCSEVSDETGVLVKPCLDMAASEFWDPEKEKYIYSKEGVERDTAEQ
                     VEYVAELIDTYGMYYVEDPIQEGDMEGLAQLTRKAGDQCLICGDDTFVTNAAILEEGI
                     RVGAGNAIIIKPNQIGTLTDTYQTVELARTHGYTPVVSHRSGETTDETIAHLAVALNC
                     PIIKTGALGGERIAKLNELIRIEEELSSAQMADLEFLRS"
BASE COUNT          287 a          459 c          419 g          436 t
ORIGIN      
        1 catactgccg ggtggatacc accaggatgt cggatggatc gtacttggcc aggaatttag
       61 ccgcagagat tatacggtca ttggttccac gcacatccag gacgtagagt ccgtcggttc
      121 ggacccggta gatgtagtgt tccatgtcct tggtcttctg ctgggttcca atgtgcagtc
      181 cagctgctag gtacttatca agtgggatta gtagttctga caaattaaca cctcataata
      241 attaactatg aaaagaccat cttagtcttc cactttcacg gcttcgttgg ggcacacatc
      301 catgcatact tcgcataggc tgcagtcctc cacgttctgg accacgactt tatcgccatc
      361 caggattaac acttccatgg ggcacacatc cacgcactcg gcgcagtcag ccccttcaca
      421 cttgtcttgg tcaatggtta ctttaaccat aaatatcttc actccatttc attttcactc
      481 taggatctga gaaactccag gtctgccatc tgggcactgc tgagttcctc ctcgatacgt
      541 ataagttcgt taagtttagc tattctctca ccgcccaggg ccccggtctt gatgatgggg
      601 cagttaaggg ccactgccag gtgggctatg gtctcgtcgg tggtttcacc ggatcggtgg
      661 cttaccaccg gggtgtagcc atgggttcgg gccagttcca cggtctggta ggtgtcggtg
      721 agggttccga tctggttggg cttgatgatg atggcgttac cagcacccac cctgattcct
      781 tcctctagga tggcggcgtt ggttacgaag gtgtcgtctc cacagataag gcactggtcc
      841 ccggccttcc tggttagctg ggccaggccc tccatatcac cctcctgtat ggggtcctcc
      901 acgtagtaca taccgtaggt gtcgatgagt tctgccacgt attccacctg ctcggctgtg
      961 tccctttcca caccctcctt gctgtagata tatttttcct tctccgggtc ccagaattca
     1021 ctggctgcca tatccaggca gggcttcacc agcaccccgg tttcatcgct cacctcgctg
     1081 caggagctgg cctgtatctc caatgcctcc tggttgctga ggttaggcgc ccatccaccc
     1141 tcgtcacctt ttcctccggt gaaaagtttg tccttggctt gtatcttctc ccggatgcgg
     1201 cggtggacgg cggtgttggt gaacactgcc tcggtgatgt cctctgcccc cactggtagt
     1261 accaggaatt cctggatgtc cggtgcgttt ttcccggcgt gggctccccc atttatcatg
     1321 ttacccaggg ggtaggggat gctggtgggg aggtttccgc ccaggaaccg gtagaggggt
     1381 aggttgtaac tggatgccgc ggccttggca gtggccatgg acaccgccac ggtggtgtta
     1441 ccccccaggg aggataggtt ctcggtgccg tctatttcct ttaaaaccag gtcgatctcc
     1501 ctgaggtcct cggcgtccag gccgatgagc tccgtggata taacgtcctc tagttctcct
     1561 attatcttat caacccctcc ttctgggaag gccaccactt c
//
LOCUS       DAOQVW010000409         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_105578_length_1592_cov_1.775537, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000409 DAOQVW010000000
VERSION     DAOQVW010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105578_length_1592_cov_1.775537"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>222
                     /locus_tag="PKK85_08035"
     CDS             <1..>222
                     /locus_tag="PKK85_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNS26003.1"
                     /translation="MIPALDFIIMQQRMYRPEGGSIRRITEVAEVVGMEEGNVQLNRV
                     FEWDNVNDKVEYVGIASQTLRDISELRGIG"
     assembly_gap    223..232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            430..>1592
                     /locus_tag="PKK85_08040"
     CDS             430..>1592
                     /locus_tag="PKK85_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNS26004.1"
                     /translation="MAPFDGLKRFFNTIGNFTINSSQKVGEGVQAPVKKLSEMRPAER
                     RLREKRGLEFGKKSEPGEKTETKPLLDASTPSKGLFGDRESKGLFGDKESKSSFQAPR
                     PQRTIKRKKMDKEEIEMFKDLIDSRHEQTKESEVDKKKQDALRKASLEEILKEEQKEK
                     MDPKLILLMGGVSFALVLVIVTVMGFGIEIGLVVGILIFMMALMIFFLPRMKQGSKST
                     EAARELPFALRQMATELRAGIGLHDSMRSIATSGYGPLSEEFSRALEEIRYGETTEKA
                     LLDMSDRINSEGMERAIHQITRTLTSGGDLAKTLTVIADDIAYEMRMKLKDYAQKLNS
                     FTMIYMFIAILAPVITLIMLIAASTVMGAILPPVVLLIMYLFFFPAIVVFMAF"
BASE COUNT          520 a          312 c          386 g          364 t
ORIGIN      
        1 aatgatacct gccctggact tcatcatcat gcaacagaga atgtacaggc cagaaggagg
       61 ttccatacgt agaatcaccg aagtagcaga ggtggttggt atggaagagg gtaacgtgca
      121 gctcaacagg gtatttgaat gggataatgt caatgataaa gtggagtacg tgggaatagc
      181 cagccagacc cttcgggaca tctctgaact acggggtata ggnnnnnnnn nnatcagaga
      241 tagaagaaga gatagaaaaa agaagactgg tactggaata cctggctgat aacaacatcc
      301 gttccataga tgaagtggga cagtatatca atcaatatta ccgtgatcca gatgcaatgc
      361 tggatgaagt attataataa tttgtggatt taaaaataat tagaaaaaat tcacttgatg
      421 gtgaaaataa tggcaccctt cgacggtctt aagagatttt ttaacaccat tggaaatttt
      481 accataaatt ccagtcagaa agtaggtgaa ggagttcagg ctcctgtaaa aaaattaagt
      541 gaaatgcggc ctgctgagag aaggttaagg gagaaaagag gactggaatt tggtaaaaaa
      601 tccgaaccag gagaaaaaac agagaccaag ccattactgg acgcgtcaac cccttcaaaa
      661 ggattattcg gtgatagaga atcaaaagga ttatttggag ataaagaatc aaaatcatct
      721 ttccaggccc cccgtcccca gcggaccata aaaagaaaga agatggataa agaagaaatc
      781 gagatgttca aggacctcat cgacagcagg catgaacaga ccaaagaatc tgaggtagat
      841 aagaagaaac aggatgccct ccggaaagct tccttagagg aaatactcaa ggaagaacag
      901 aaggaaaaga tggatcccaa gctgatcctg cttatgggcg gggtttcctt tgcattggtg
      961 ctcgtgatag taactgtgat gggctttggt attgaaattg gcctggtagt gggaatcctc
     1021 atatttatga tggccctcat gatcttcttc cttcccagaa tgaaacaggg aagcaaatcc
     1081 acagaagccg ccagagaact gcccttcgcc ttgaggcaga tggccacaga attaagggcc
     1141 ggaataggac ttcacgacag catgcgctcc atcgccactt ctggatacgg acccctatct
     1201 gaagagttct cccgggcact ggaagagata aggtacgggg agaccacaga aaaagccctc
     1261 ctggatatga gcgacagaat aaactctgag gggatggaaa gggctatcca tcagataacc
     1321 cgaacactca ccagtggtgg ggatttagct aaaactttaa cagttatagc tgatgacatc
     1381 gcctatgaaa tgcgtatgaa gcttaaagat tacgctcaga aattgaattc attcaccatg
     1441 atctacatgt tcatcgccat actggcaccg gtaattaccc tgatcatgtt gatagccgca
     1501 tcaacagtta tgggcgccat actgccgcca gtagtcttgt tgataatgta cttattcttc
     1561 ttcccagcca tagtggtgtt catggccttc at
//
LOCUS       DAOQVW010000410         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_105836_length_1589_cov_3.665580, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000410 DAOQVW010000000
VERSION     DAOQVW010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105836_length_1589_cov_3.665580"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKK85_08045"
     CDS             <1..630
                     /locus_tag="PKK85_08045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNS26005.1"
                     /translation="LPALDDAVEGMEAEEEKTVEISPENGFGKRDPNLFQMVPMREFK
                     KQGIKPQVGMAITSEGHTGIIRTVSGGRVTVDFNHELAGKNLEYQVKVEEIIEDDTEK
                     VKAMIELHYPNPNLDPEKHTIRVEDGKAVIYMDEMAKFDQQKSYMDITMARFRIARDI
                     YDNMDNVSKVEFVDVFEKKEEATEEEEVPEKLPEEEVVEEKLPEEREDQ"
     gene            complement(724..>1589)
                     /gene="serS"
                     /locus_tag="PKK85_08050"
     CDS             complement(724..>1589)
                     /gene="serS"
                     /locus_tag="PKK85_08050"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNS26006.1"
                     /translation="AVEEIFLEKLVYKLGFEECMWPKLIPLPVLNKMRYLEGLPEGMY
                     YCSAPRRDPELFEKFKNELTITKEVPINRLKDGLKDPSYVLAPAQCEPFYEFFSHEVV
                     DEEDLPISLFDRSGWTYRWEAGGAKGLDRVHEFQRVELVWLGTPEQVEKIRDTTLEIS
                     QNLADLMELEWYTEVGDDPFYLEGRKSEERGIEFPDVPKYEMRLVVPGADKGVAAVSA
                     NVHGTHFTEGFSIKEAHQKTLWTGCTGIGITRWLFGFLAQKGFDKDNWPVGVKERMRK
                     VQVPRMLTWPG"
BASE COUNT          450 a          389 c          366 g          384 t
ORIGIN      
        1 ttacccgcat tagatgatgc tgtggagggt atggaagctg aagaagaaaa aactgttgaa
       61 ataagccctg aaaatggatt cggcaagcgg gaccccaacc tcttccagat ggttcccatg
      121 cgcgagttca aaaaacaggg gatcaagcct caagtgggaa tggccatcac atccgagggc
      181 cacactggta tcatccggac cgtcagtgga ggccgggtca cagtggactt caaccacgaa
      241 ctggccggaa agaacttgga gtaccaagtt aaggtggagg agatcatcga agatgacact
      301 gaaaaggtca aagccatgat cgagttacac taccccaacc ccaacttgga ccctgaaaaa
      361 cacacgatcc gggtggaaga tggtaaagca gtcatttata tggatgaaat ggctaaattc
      421 gaccaacaaa agtcttacat ggatatcacc atggcaagat tccgtatcgc cagggatatc
      481 tacgataata tggataatgt ttccaaagtg gaatttgtag atgttttcga gaagaaggaa
      541 gaagccactg aagaagagga agttcctgaa aaattaccag aagaagaagt tgtggaggaa
      601 aaacttcctg aagaaagaga agaccaataa atgaaatggg acgtagaaac ctatttctac
      661 cccctaccta tttaccatct ttgatggtgg aagcctaaat tttttctaag attatttggt
      721 gtactaccca ggccaagtaa gcatcctggg aacctgaacc tttctcatcc tttcttttac
      781 tccaactggc cagttatcct tatcaaatcc cttctgagcc aggaatccga acaaccagcg
      841 ggtgatgccg ataccagtac atccggtcca cagggttttt tgatgggctt ctttgatgga
      901 aaaaccttcc gtaaagtggg tgccgtgtac gttggctgaa actgcagcca ctcccttatc
      961 agcaccagga accacaaggc gcatctcata tttgggtaca tccgggaact caatccctct
     1021 ctcttctgat ttcctaccct ccaagtagaa gggatcatca cccacttcgg tgtaccattc
     1081 cagttccatc agatcagcta ggttctgtga aatttccaga gtggtgtcac gtattttctc
     1141 cacctgttcc ggggtgccca accacaccag ctccaccctc tggaactcat gaacccggtc
     1201 caatcccttg gcaccgccgg cctcccagcg gtaggtccag ccactacggt caaagaggga
     1261 gatgggaaga tcctcctcat ctaccacttc atgtgagaaa aattcgtaaa aaggttcgca
     1321 ctgggctggt gccagaacgt aggaggggtc cttcaacccg tccttcagac gattgatggg
     1381 cacttctttt gtgatggtta actcattttt aaatttctca aaaagttctg ggtcacgacg
     1441 aggggcagaa cagtagtaca taccttctgg taggccttcc aggtaacgca ttttgttcaa
     1501 cactggtaag ggtatgagtt tgggccacat acactcctca aagcccagtt tgtataccag
     1561 tttctccagg aatatctctt ccactgccc
//
LOCUS       DAOQVW010000411         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_106122_length_1587_cov_2.587467, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000411 DAOQVW010000000
VERSION     DAOQVW010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106122_length_1587_cov_2.587467"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKK85_08055"
     CDS             <1..357
                     /locus_tag="PKK85_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNS26007.1"
                     /translation="QEEFGGVVPDKMKELLLLPGVGRKTANCVLVYAFQKPAIPVDVH
                     VHRISNRLGLVETRTPEETELELERLVPREYWIELNDLLVQFGQTICRPVNPRHEICP
                     LADICACYQDILEGNP"
     gene            987..1454
                     /locus_tag="PKK85_08060"
     CDS             987..1454
                     /locus_tag="PKK85_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26008.1"
                     /translation="MNTKALVGLAVLIVVIGGVSAFGYFSPAEKTSPTGQSSAPAAPQ
                     NLEEDKSTQNTVNAQGQVCTKCGGKGYLKCGKCGGDGVIGGKCAKCGGDGKVYYDENC
                     NEQPNVVKMLQVLACHEATCPSCGGTGGTQSKCPACGGDGKITCPGCGGDGHT"
BASE COUNT          428 a          358 c          383 g          418 t
ORIGIN      
        1 caggaggagt ttggtggggt ggtgccggat aagatgaagg aactgttact cctcccgggg
       61 gtgggccgta aaaccgccaa ctgcgtcctg gtctacgcct tccagaaacc tgccataccc
      121 gtggatgtgc atgtgcaccg gatcagcaac cgcctgggac tggtggagac caggaccccg
      181 gaagagactg aacttgaact ggaaagactg gtcccccggg agtactggat tgaactaaat
      241 gacctcctgg tgcagttcgg ccagaccata tgccggccag tgaacccccg acacgagata
      301 tgtccactgg cagatatctg tgcatgctac caggatatcc tggaaggtaa cccttaaacc
      361 tggggaaagt cctgggagta aatggtctaa aagagaaacg cagatccttc agtggatact
      421 tggcctttgg agcactgaac tccccagcca ttggatttac aatgggacat ttaaaattat
      481 ccctatccta tatttttgcc aggtttttgg ctctatccat taggtggggg aatttggatc
      541 ttaaaaaaat ttatatgtta gcacttacta agaaattgaa ggggggaacg aatgggtaat
      601 ttgggaaaaa accagttaat tggtttttct tttggagtgt acattcatca atctacactc
      661 ccacccatcc cttttagact ggataatcat tctagtggac agtgttgatt attgttttgc
      721 aagtatcctt aaatattcca ttaccccctg gatctgccaa ccactgcttc tcatatcatg
      781 gttgatctgc actggtccca tgccaagccc actgtgatgg atactgctag ttgcagggac
      841 acagtccctg ccattctccc tatcaatgtt tttttactct ctaagcctgc accatccatg
      901 atccaaatat atattcataa caaccaacaa tattggcatt aatattaaat actatttatc
      961 ataataaatt atgatggtga tgctatatga ataccaaggc attagtaggt ttagcagtgt
     1021 tgatagttgt aattggcgga gtcagtgcct ttggatattt ctctccggct gagaaaacca
     1081 gcccaacagg acagagttct gctccagccg ccccccagaa tttagaagaa gataaaagca
     1141 cccagaacac ggtgaatgcc cagggacagg tatgtaccaa gtgtggtggt aagggttacc
     1201 tgaagtgtgg aaaatgtggt ggtgacggag tcatcggagg caaatgtgcc aagtgtggag
     1261 gtgatggaaa ggtttactac gatgaaaact gtaacgaaca acccaacgtc gttaaaatgt
     1321 tacaagtcct ggcctgtcac gaggccacct gtcccagctg tggcggaacc ggcggtaccc
     1381 agtccaaatg tccggcctgt ggaggggatg gtaagatcac ctgcccgggt tgcggtggag
     1441 acggacacac ctgaaactat ttattttttt aatttccctt ttttacttta tatgagaact
     1501 gattataccc cccaaaaatt ccgggtggaa tacgggcccc tccattaccc catggaggga
     1561 tttacaatct ccctgaaaga tggtgtt
//
LOCUS       DAOQVW010000412         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_106502_length_1583_cov_4.398560, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000412 DAOQVW010000000
VERSION     DAOQVW010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106502_length_1583_cov_4.398560"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKK85_08065"
     CDS             <1..433
                     /locus_tag="PKK85_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS26009.1"
                     /translation="KTQKMLMSGYLGTMGFGLPAALAAQLVYPDRQVACITGDGGFSM
                     VMADFLTAVKYELPVKVFLMNNHQLAMIMQEQKVENYPNWQTQLHNPDFASYAQECGG
                     TGIRVKDPEKLRAAISKALNKEGPVLVDIETDPRRFIQASD"
     gene            474..>1583
                     /locus_tag="PKK85_08070"
     CDS             474..>1583
                     /locus_tag="PKK85_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNS26010.1"
                     /translation="MVQDAQRLRSKIEDTPENRAKCHCPLCPSYPHECEGELLYCKLG
                     ASDADINAHGCICDTCTIYYEYGLKGIYYCDKMEAGQSKTNIRKISKRENAFSYQEVV
                     DIKDKSAGMEMVASMGSQKELPFKLDDLHFIPAQLSRIPVNADEPVNTTVVIGPEAKK
                     PFKVSSPLLISGMSFGAVSRNVRLVIGRVASELGLGFNSGEGGVLEEENQFSDYMIVQ
                     YSTGRFGLKEELMKNAAAIEIRFGQGAYPGKGSYLPADKITSEVARIRGLKPGEAAYS
                     PAHHPDINNPQELQEKIATLKNLAEGSPVGAKIGCGNIEDDVKILAEAGIDFIALDGF
                     GGGTGATSPYIRDNVGIPIVAALPRAYKVLLKMGLK"
BASE COUNT          473 a          299 c          389 g          422 t
ORIGIN      
        1 aaagacccag aaaatgttga tgtccggtta tctgggaacc atgggattcg ggctccccgc
       61 tgctctggct gctcaattag tctatcctga tagacaagtg gcttgtatta ctggggatgg
      121 tggtttttcc atggtcatgg ccgatttttt aacggcagtt aaatatgaac ttccagtgaa
      181 agtattcctg atgaacaatc accagttggc catgatcatg caggaacaga aggttgaaaa
      241 ctatcctaac tggcagaccc aacttcataa ccctgatttt gccagttacg cccaggaatg
      301 tgggggaaca ggtatccgtg tgaaagatcc tgaaaaactc agagccgcca tttctaaggc
      361 tctgaataag gagggaccag tgctggtgga tattgaaacc gacccccgaa ggttcataca
      421 ggcgagtgat taaaggttaa caaattctat tattatgaaa aggagaagtc aacatggtcc
      481 aggatgctca aagattaagg agtaagatag aagatacccc tgaaaatcgg gccaagtgtc
      541 actgcccttt atgtcctagt tatcctcacg aatgtgaagg tgagttgttg tactgcaagt
      601 taggggcaag tgacgctgat ataaatgctc atggttgtat atgtgatacg tgtaccattt
      661 attatgaata tgggctaaaa ggaatctatt actgcgataa aatggaagca ggacagagta
      721 aaacaaatat tcgtaaaatt tctaagagag aaaatgcctt ttcttaccaa gaagtggtgg
      781 atattaaaga taagagtgct ggtatggaga tggtggcatc catggggtcc cagaaggagc
      841 tgccatttaa acttgatgat ttgcatttca taccagccca gttatcccgt attccagtaa
      901 acgcagatga accagtaaat accactgtag tcataggtcc tgaggcaaaa aaaccgttta
      961 aagtatcatc acccctttta atttctggca tgagttttgg ggcggtttcc aggaatgtta
     1021 gacttgttat tggacgagtt gcctcagaat tgggcttggg attcaactct ggtgagggcg
     1081 gtgttttaga ggaagaaaat cagttttctg actacatgat agtccagtac tccaccggaa
     1141 gattcggttt aaaggaagaa ctaatgaaaa atgcggcggc cattgaaatc cgatttggtc
     1201 agggtgctta tcctggtaaa ggtagttatc taccagcaga taagatcaca tccgaagtgg
     1261 cccgtatcag aggtctaaaa ccaggggaag ctgcgtactc tcctgctcac catcctgaca
     1321 ttaataatcc ccaggaactg caagaaaaaa tagccactct aaaaaattta gctgaagggt
     1381 ctccagttgg tgccaaaata ggttgtggta acattgaaga tgatgttaag attttggcag
     1441 aggcaggtat tgattttatt gctcttgatg gttttggagg gggaactgga gccacatcac
     1501 cttacatcag agacaatgtc ggaattccca tcgtagctgc acttccccga gcctataagg
     1561 tcctgttgaa aatggggcta aaa
//
LOCUS       DAOQVW010000413         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_106780_length_1581_cov_2.780472, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000413 DAOQVW010000000
VERSION     DAOQVW010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106780_length_1581_cov_2.780472"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKK85_08075"
     CDS             complement(<1..815)
                     /locus_tag="PKK85_08075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNS26011.1"
                     /translation="MTSSDYYKDKISIVTGGNSGIGYALCEELLIRGAIVYMAGRNPQ
                     KVSDAAKKLSEYGDRIHTVIVDVTVQEQVQRAIEDIAAKEGRLDMLFNNAGVGGTLPF
                     TFATLEDWKNIIDTNIWSVIYGVYTAVPIMLKQGSGHIINTSSIAGILPPPFQSLYCL
                     TKFGVTGLTESLKYEYADRGLNFSTICPGFVASAIYNKGVDGKPKGELKIPADAYPAD
                     KAAIDILDGVQKHDGIICVPNELKEDWKDYVLGKGDESLLEVARERREGYKKIL"
     gene            complement(838..>1581)
                     /locus_tag="PKK85_08080"
     CDS             complement(838..>1581)
                     /locus_tag="PKK85_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HNS26012.1"
                     /translation="GGKFDPNETIHQKSTSLNISYETDAKILENFIPEGFELLDPVVD
                     VGFSKFSEINWMQGGQYNVVNVAVPVRFHGKKDELDGIYTLIVWENRTAPILGGREQT
                     GQPKVFADIEDLHILKPHYSTNASYEGNTFLNMEFEATEEVTGKKLEEVKPKFASINN
                     IGWRYIPKVGGPGPDLSQFILYPQGVDVKCAQTGKGNLKWTELTPMQNPSQWWMINSL
                     AALPIKKITQAILTEGNATLHSRGSRIIE"
BASE COUNT          430 a          348 c          273 g          530 t
ORIGIN      
        1 agaatttttt tatatccctc tctgcgttca cgtgctactt ctaataaact ttcatcccct
       61 tttcctagga cataatcctt ccaatcttct tttaactcat ttgggacaca gatgatgcca
      121 tcatgttttt gaactccatc taatatatct atggctgctt tatcagcagg ataggcatca
      181 gctggtattt tcagttctcc tttaggtttg ccatcaactc ccttattgta aatagctgaa
      241 gccacaaaac ctgggcagat agttgagaaa ttaagacccc tatctgcata ttcatatttt
      301 aatgactcag taagtccggt tacaccaaat ttggttagac agtagagtga ctggaaggga
      361 ggggggagaa taccggcaat ggaactggtg ttaattatat gtccagatcc ctgttttaac
      421 atgatgggta cagcggtgta aacaccgtaa ataacactcc aaatatttgt atcaattata
      481 tttttccaat cctccagggt agcaaatgtg aatggaagag taccaccaac acctgcatta
      541 ttaaataaca tatctaatct accctccttt gctgcgatat cttcaatggc tctctgcacc
      601 tgttcctgaa ctgtcacatc tactatgact gtatgaatcc tatcaccata ttcagaaagc
      661 ttttttgcag catctgaaac tttttgaggg ttacgccctg ccatgtaaac gattgcccct
      721 cttatgagga gttcttcaca taaagcatac ccaataccgg aatttccgcc ggtaacaata
      781 gatattttgt ctttataata atctgaacta gtcataaaat cacctctccc ctttttttta
      841 ttcaatgatc ctggatcctc gagagtggag ggttgcattt ccttcagtta gtatggcttg
      901 agttatcttt tttataggta aggcagcaag actgttgatc atccaccact gtgaaggatt
      961 ttgcatgggt gtcaattcag tccatttaag atttcctttt ccagtttgag cacatttgac
     1021 atctacacct tgaggataca gaataaattg acttaaatca ggtccaggac cacctacttt
     1081 aggaatatat ctccagccaa tgttgttgat gctggcaaac ttgggtttta cttcttccaa
     1141 tttttttcct gtcacttctt cagttgcttc aaattccatg ttcaagaaag tgttaccctc
     1201 ataactggca tttgtggaat aatgaggttt taaaatatgc agatcttcaa tatcagcaaa
     1261 aacttttggc tgtcctgttt gttcacgacc ccccaggata ggggctgtcc tattttccca
     1321 gacaatcaat gtataaattc catccaattc gtcttttttc ccatggaagc gaacgggtac
     1381 tgctacattc accacgttgt attgtccacc ctgcatccag tttatctcac taaatttgct
     1441 gaaaccaaca tcaaccacag gatccagtaa ctcaaaccct tctgggatga agttttcaag
     1501 aatttttgca tcagtctcat aactgatgtt aaggctagtt gatttttgat ggattgtttc
     1561 atttggatcg aattttcctc c
//
LOCUS       DAOQVW010000414         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_106863_length_1580_cov_3.237377, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000414 DAOQVW010000000
VERSION     DAOQVW010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106863_length_1580_cov_3.237377"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKK85_08085"
     CDS             <1..437
                     /locus_tag="PKK85_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512071.1"
                     /note="Directs the termination of nascent peptide
                     synthesis in response to the termination codons UAA, UAG
                     and UGA; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNS26013.1"
                     /translation="MDVLTEIDIMREKKLVQRFLSELVDNNGLYSYGEKQVRQNLQMG
                     AVEVVLLSEELSSERSSYQCQSCGHVEEKTVKKESEEDISCPSCGDTMKPSRSRDLID
                     DFVEMAEEVGSEVEIISTETEEGIQLYRAFGGIGAILRYRVV"
     gene            complement(506..1102)
                     /locus_tag="PKK85_08090"
     CDS             complement(506..1102)
                     /locus_tag="PKK85_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HNS26014.1"
                     /translation="MNQKLIKKADDLRGRGFTTGEIADELNVSKDTARWLILQGTNKK
                     TKEREEAPLDFAINWNSLGGSSTRIGYVSAALADMAAQYGEVEVIVGITVSGIPLATM
                     MASILEADLAVFHPIKHRKRKDAGGAISSNFASVEGKKVVIVDDVITSGRTIGEAIKV
                     LRNLKSQPIAAVVLIDKKGIPELEDVPVESLIRVSRLG"
     gene            complement(1115..>1580)
                     /locus_tag="PKK85_08095"
     CDS             complement(1115..>1580)
                     /locus_tag="PKK85_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNS26015.1"
                     /translation="GVTIDLAIHEVDVMAYLLDSPASKVYAHVGSRLEKCEFEDHAEI
                     MMEFYNGASGVLEVNWLTPYKQRKLEVTGTEGILALDYIDQDVEIFGKNARKVRVPHH
                     EPLMEELNSFLNSIIDDEKPKITGEDGMHALKVVLAAMESAKGKVPVDLEIE"
BASE COUNT          415 a          385 c          320 g          460 t
ORIGIN      
        1 ccatggatgt tctaactgaa attgacatca tgcgggagaa gaaactggtt caaagatttt
       61 tatcggaact ggtggataac aatggattgt actcctacgg agaaaagcaa gtccgacaaa
      121 acctgcaaat gggtgccgtg gaagtggtgt tattgtccga agaactaagc tccgaacgtt
      181 catcttacca atgccagtct tgtggacacg tggaagaaaa aactgtcaaa aaagaatccg
      241 aagaagacat ttcctgccca tcttgtggtg atacaatgaa gccgtcccgt tcaagggatc
      301 tgatagacga ctttgtggag atggccgaag aagttggttc agaagtggag atcatatcca
      361 ccgaaactga agaaggaata cagttgtacc gtgcttttgg cggaattggc gccatattaa
      421 ggtaccgagt ggtttaactg gataattttt aaaaaaataa gttttatggt gattttaacc
      481 ccttctattt ttttataatt acaaatcatc ccaggcgact cacccttata agagactcta
      541 ctgggacgtc ttccagttcg gggattccct tcttatcgat gagcaccacc gcagcaatgg
      601 gctgtgactt aagatttcgc aggaccttaa tggcttcacc gatggttctt ccgctggtta
      661 tgacgtcatc cacaataacc accttttttc cctccaccga ggcgaagttg ctgctaatag
      721 cccctcccgc atcctttctt ttacgatgtt ttatagggtg gaagacagcc aagtctgcct
      781 ccagtatgct ggccatcatg gtagccaggg gtatcccgct aacggtaatt cccactatta
      841 cttcaacttc tccatactga gctgccatgt ctgctaaagc tgcggaaacg tatccaattc
      901 ttgtggaact tccacctaga ctattccagt ttatggcaaa gtccaaaggt gcttcttccc
      961 tttctttggt cttcttatta gtgccctgaa gtatgagcca acgtgcagtg tctttagaca
     1021 catttagttc atctgcaatc tctccagtag taaatcctct acctctaagg tcatcggcct
     1081 tttttattag tttctgattc atagctaccc cttcttattc aatttccaga tcgactggta
     1141 cttttccctt ggcagactcc atggcagcca acaccacctt cagagcatgc ataccatcct
     1201 caccagtaat ttttggtttt tcatcatcga ttatggaatt caaaaaggaa tttaactcct
     1261 ccattagagg ttcatgatgg gggaccctta ccttccgggc atttttacca aatatctcca
     1321 catcttggtc tatataatcc agggctaata tgccctcggt tccagttact tccagcttcc
     1381 tttgcttata aggagtgagc cagttaacct ccagaacacc gctggcgcca ttgtagaact
     1441 ccatcattat ctcagcatgg tcttcaaatt cacacttctc taaacggctg cccacatggg
     1501 cataaacttt agaagcagga ctgtccagaa ggtatgccat aacatccacc tcgtggatgg
     1561 ccaagtcaat ggtaactccc
//
LOCUS       DAOQVW010000415         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_106909_length_1580_cov_2.546885, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000415 DAOQVW010000000
VERSION     DAOQVW010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106909_length_1580_cov_2.546885"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PKK85_08100"
     CDS             <1..1006
                     /locus_tag="PKK85_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNS26016.1"
                     /translation="SVMRAKPLPEVATILDAEDEIAAINMALGASYTGASSMVATSGS
                     GFALMTEAVGLSGMTETPVVIYIGMRPGPAVGLPTRSSQEDLDLALYSGPGEFARAIF
                     APGRLEDAYQLSQRAFQLAEEYQIPVFILSDQYLADLHYNLPEEDLVDLKWESYLVKT
                     EEDYQRYQLTRNGISPRGVPGYGGGLVVVDADEHDEDGHITEDLELRSRMVEKRYHKL
                     QNLRKEALPPTLYGEEDCEVLLVGWGSTYHPLEEARRKLDKDDLGFLHFQQVYPLHPD
                     TEKYLAGKKTVAYENNIKGQFSNLLKLEYGVDIQERVAKYNGMPFSVEEVLRSVREVL
                     "
     gene            1006..>1580
                     /locus_tag="PKK85_08105"
     CDS             1006..>1580
                     /locus_tag="PKK85_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS26017.1"
                     /translation="MKPELYDQPDADVAWCPGCGNFGILRALKLALSDLEIEPEKLVL
                     VSGIGQAGKLPHYLKSNVFNGLHGRSLSPATGIRASNPGLTVVDVSGDGCMYGEGGNH
                     FLHTIRRNVDLTNLVHNNMVYGLTKGQASPTSQRDFRTPLQVEGVFEEPFNPLSVAIA
                     LGAGFVARAFAGDVDKTREIIKQAILHRGYAL"
BASE COUNT          361 a          428 c          485 g          306 t
ORIGIN      
        1 ctctgtcatg agggcaaagc cactcccgga ggtggccacc atactggatg ccgaggatga
       61 gatcgccgcc ataaatatgg ccctgggggc ttcctacacc ggggcatcca gtatggtggc
      121 cacctccggg agtggctttg ccctcatgac agaggcagtg ggcctgtccg ggatgaccga
      181 aaccccggtg gtgatctaca ttggcatgcg tccaggacca gcggtgggcc ttcccacccg
      241 cagctcccag gaggacctgg acctggccct ttacagtggg ccgggagaat ttgcacgggc
      301 catcttcgcc ccggggaggc tggaagacgc ctaccagttg tcccagcggg ccttccaatt
      361 ggcggaagag tatcagatcc cggttttcat actcagtgac cagtacctgg ctgacctgca
      421 ctacaacctc ccagaggagg atctggtgga cctgaaatgg gaaagctacc tggtaaagac
      481 agaagaagac taccagcgtt accagttaac caggaatggt atctcccccc ggggggtgcc
      541 tggctatgga gggggactgg tggtggtgga cgcagatgaa cacgatgagg atggtcacat
      601 caccgaggac ctggagttaa gatccaggat ggtggagaag cgctaccaca aactccagaa
      661 cttgaggaag gaggccctgc ccccaaccct ctacggcgag gaggactgtg aggtgctact
      721 ggtggggtgg ggctccacct accaccccct ggaggaggcc aggaggaagc tggacaagga
      781 tgatcttggc tttttacact tccaacaggt gtatcccctg caccctgaca cagagaagta
      841 cctggcaggt aaaaagacag tggcctatga gaacaacatc aagggccagt tttctaatct
      901 cctgaaactg gaatacgggg tggacatcca ggaaagagtt gctaagtaca atgggatgcc
      961 cttctcggtg gaggaagtgc tgcggagtgt aagggaggtg ctatgatgaa accagagctt
     1021 tatgaccagc cagatgctga tgtggcctgg tgtcctggtt gcgggaattt cgggatccta
     1081 agggccctga aactggccct atccgacttg gagattgagc cagagaaatt ggtactggta
     1141 tccgggattg ggcaggccgg gaaactcccc cactacctga aaagcaacgt tttcaatggg
     1201 ctgcatggcc ggtccctatc cccagcaaca ggtatcaggg cctccaatcc agggcttacg
     1261 gtggtggatg tcagtggcga tggctgcatg tacggggagg gtggtaacca cttcctgcac
     1321 accatccgcc gcaacgtgga cctcaccaac ctggtgcaca acaacatggt ctacggctta
     1381 accaagggac aggccagtcc caccagtcag cgggacttcc gcacccccct gcaggtggag
     1441 ggagttttcg aggagccctt caacccctta tccgtagcca tagccctggg tgccggtttt
     1501 gtggccaggg cctttgccgg ggatgttgat aaaacccggg agatcattaa acaggccatc
     1561 ctgcataggg gctacgccct
//
LOCUS       DAOQVW010000416         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_106977_length_1579_cov_4.068241, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000416 DAOQVW010000000
VERSION     DAOQVW010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106977_length_1579_cov_4.068241"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PKK85_08110"
     CDS             complement(<1..988)
                     /locus_tag="PKK85_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNS26018.1"
                     /translation="MFIGMRKTILLMIISFFCILSIISTSDAHILIIGDSLSDYPQTL
                     QETQNIAHQLKLRGYNVLELYQKNATTKNILKGMYDADAIIYAGHGGYQYGNYDNKGG
                     TATGPFSLVGAPSSSIDSSNFIWGVGGQMREGAGSLFEAPFKANIPVFLLHACFSTGW
                     VGDKEVSNPAETIYNFSRMFTSVGANYYATAWNGAEIIYDFLNGASNFLEANQQNYEP
                     LTTSTTYNGVNIWKNQHGYSAFVGNWQGSFPTVRQTTVYDDTAAEDWYNGNRIREDLR
                     CSFTVIPGPYYTQHPVTFTEGSYSMASNITTYFWNFGDGTTYQSNSSSNPVHT"
     gene            complement(1300..>1579)
                     /locus_tag="PKK85_08115"
     CDS             complement(1300..>1579)
                     /locus_tag="PKK85_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNS26019.1"
                     /translation="DGLGDIETIRKISQGSPNIRDAIINGEVALIINTPSGKQSADDG
                     YYIRRMAIELNIPYVTTLAGARAALNAIENVEDGKIGVKSLGEYHHEP"
BASE COUNT          445 a          298 c          331 g          505 t
ORIGIN      
        1 aggtatgcac aggattagaa gaactgtttg attgataggt agtgccatct ccaaagttcc
       61 agaagtaagt ggtgatgtta ctggccatac tgtaggaacc ttcggtgaag gttactggat
      121 gttgggtgta gtagggaccg ggaattactg tgaaagagca tcttaaatct tcccggatac
      181 gattgccatt gtaccagtcc tctgctgctg tatcatcata aacagtggtt tgtctgacgg
      241 tagggaagct accctgccag ttgcccacaa atgcagaata tccgtgctgg tttttccata
      301 tgttaacacc gttataggtg gtgctggtgg ttaaaggttc gtagttttgt tgattagctt
      361 ccaggaagtt tgaggcgcca ttaaggaagt cgtatatgat ttctgcccca ttccatgcag
      421 tggcatagta gttagcacct acgctggtaa acatccttga aaaattgtat atggtttctg
      481 caggattaga aacttcttta tcccccaccc atccagtgga gaaacaagca tgaagtaaaa
      541 aaactggtat attagccttg aaaggggctt cgaacagact tccagcccct tctctcatct
      601 ggcctcctac tccccaaata aagttactgg agtcaatgga actggatggt gccccaacca
      661 gggagaatgg gcccgtagct gtgccacctt tgttatcgta gttcccatac tgataaccac
      721 catggcccgc ataaataatg gcgtctgcat catacatgcc tttcaggata tttttggtag
      781 ttgcgttttt ttgatatagt tccaggacat tgtaacccct taatttcaac tgatgcgcta
      841 tgttctgggt ttcttgcaga gtctggggat aatcagatag ggaatctcca ataataagaa
      901 tatgggcatc tgaagtggat atgatactta aaatacaaaa aaaactaatg atcatcagta
      961 aaatggtttt tctcatccct ataaacatta tactcaccgt cacagccttt gatggtagga
     1021 ttgatgaagc acggtattaa ttggttctta cttgtaataa tattatatgt tgtttttaac
     1081 tattaatctt gtctattgaa tctaattttg acttttacta ttaagatatt atttcatagc
     1141 ctctactcat ttaaatttat aagaaagaac aactgtcatt tttttaaaca aaaaaaaagt
     1201 tagttattcg gatatactta ggtttaatag tctaaattgg attaaaataa taaaaaaaaa
     1261 tttaattggt aaattgggat atctgttgga taattaatat catggctcgt ggtgatattc
     1321 acctagggac ttaacaccta ttttaccatc ttccacattt tcaattgcat ttagggcggc
     1381 acgagcccct gccagggtgg tgacataggg tatgtttaat tcaatagcca ttctacgtat
     1441 ataatatcca tcatccgctg attgcttacc agagggagtg ttaatgatca gggctacttc
     1501 cccgttgatt atggcgtccc gaatgttggg ggagccctgg ctaattttac gaatagtttc
     1561 tatgtctccc aggccgtct
//
LOCUS       DAOQVW010000417         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_106989_length_1579_cov_3.405512, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000417 DAOQVW010000000
VERSION     DAOQVW010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106989_length_1579_cov_3.405512"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKK85_08120"
     CDS             complement(<1..215)
                     /locus_tag="PKK85_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNS26020.1"
                     /translation="MEKEKDMLFLTALVAAGATLASGLAASLQWMVVLPLTLGVLLLT
                     ILIMFQYRKDAIELSEKLETGAFILVVV"
     gene            complement(246..1106)
                     /locus_tag="PKK85_08125"
     CDS             complement(246..1106)
                     /locus_tag="PKK85_08125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNS26021.1"
                     /translation="MNLMLNILLNVIIAFLLGSLLFGLQRKVMARIQKRPGPPIIQHL
                     LHTLKFYIKESSFPKTAAMPFYIAIASILCVIWVSAVIVGPVAQGSLLLIFAIYALHK
                     IVEHNAGSSSGSPYGKLSCVRAVFSAAAEVPLFAVLIIIFLKTRTMVISDIIAYQAQN
                     GALLFTIPLAALMFFVLILSKAPYSPFAITKGKDIISGYETEHFGLLRGYLMISESIA
                     WYMLLWIFLTVFLGPLSLLGYLVGMVVISVIVAFINATTPMLNPNHSIMVQVTMAFIG
                     IVGSIILLML"
     gene            complement(1116..1331)
                     /locus_tag="PKK85_08130"
     CDS             complement(1116..1331)
                     /locus_tag="PKK85_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF788 domain-containing protein"
                     /protein_id="HNS26022.1"
                     /translation="MKTLYISSYLMFIVSIAGIVYALVADPPAWIVYAISILFIPLSI
                     LSFGLLIMARSKKEEEDERAREPFIGY"
     gene            complement(1358..>1579)
                     /locus_tag="PKK85_08135"
     CDS             complement(1358..>1579)
                     /locus_tag="PKK85_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26023.1"
                     /translation="EIVKLPYVTSMMTFGSLLLLMGIIGETGVAPFYATKAEMFRTPG
                     SPFILIIHLSSLLVIVRAIEVLLIINKPF"
BASE COUNT          418 a          343 c          407 g          411 t
ORIGIN      
        1 acaacaacca ggatgaaggc tccggtttcc agcttctctg agagttctat ggcatccttc
       61 cggtactgga acatgatgag gatcgttagg agcagtactc ctaaggtcag aggcagtacc
      121 accatccact gtagtgaagc agccaggcca ctggccagag tggctcccgc agctaccagg
      181 gctgtgagga acagcatatc tttctctttt tccatggtat taattctcct tttctagatt
      241 gaccattaaa gcattaataa tatgatggat cccacgatcc caatgaaggc catggtcacc
      301 tgaaccatta tagagtggtt ggggtttaac atgggggtgg tggcgtttat aaaggccacg
      361 atgacactga tgaccaccat tcccaccagg taccctagta aactgagtgg acccagaaat
      421 accgttaaaa atatccaaag cagcatgtac caggctatgg attctgagat catcaggtat
      481 cccctgagaa gtccgaagtg ttcggtttca tagccactga taatatcctt gcctttggtt
      541 atggcaaagg gagagtaggg tgccttggat aatattaata caaagaacat cagggcagcc
      601 agtggtatgg tgaacagtaa cgccccattc tgggcctggt aggcgatgat gtccgagata
      661 accatggtcc tggttttaag gaagatgatg atcaacactg caaagagggg tacctcagct
      721 gctgctgaga aaacagcccg cacacagctg agttttccat agggagaccc tgatgagctc
      781 cctgcgttgt gctccacaat tttgtgcagg gcgtagatgg cgaatatcag gagtaatgat
      841 ccctgggcca ctggacccac aataactgct gatacccaga taacacatag gatgctggct
      901 atggctatgt agaatggcat ggctgctgtt ttggggaatg atgattcttt tatatagaac
      961 ttgagggtgt gcagcaaatg ctgtattatg ggtggtccgg gtcgcttctg aatcctggcc
     1021 atgaccttac gctgcagtcc aaatagtaga ctgcctaata ggaaagcgat aatcacgttc
     1081 aggagtatgt ttagcattaa attcatcttt tcaccttaat atccgatgaa tggttctctg
     1141 gcacgctcgt cctcttcctc tttcttgctg cgggccatga tcaagaggcc gaagctcagg
     1201 atggataggg gtatgaagag tatggagatg gcgtaaacaa tccaggcggg tggatctgcc
     1261 accagagcgt acactatgcc tgcgatggat actatgaaca tcagatagct gcttatgtaa
     1321 agggtcttca tcaagattca cctagtttct taatttctta gaatggcttg tttattatga
     1381 gtaacacctc tatggccctt acaatcacca gtagggagga taggtgtatg atgaggatga
     1441 agggtgatcc cggggtgcgg aacatctcgg ccttggtggc gtagaagggc gccaccccag
     1501 tttcgcctat gattcccatt aagagtagga gtgatccaaa ggtcatcatg gaagttacgt
     1561 aaggtaattt aactatctc
//
LOCUS       DAOQVW010000418         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_107018_length_1579_cov_2.749344, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000418 DAOQVW010000000
VERSION     DAOQVW010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107018_length_1579_cov_2.749344"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKK85_08140"
     CDS             complement(<1..627)
                     /locus_tag="PKK85_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS26024.1"
                     /translation="MEREIYQTDVLVIGSGGAGCRAAIEARQHGRDVIIVSKGLSFKS
                     GCTTLAEGGYNAAFAYVDEEDSTEAHFQDTLRGGGYLNDRKLAKILVEEAPDRLNELE
                     GFGAIFDRQESGLLNQRPFGGQTYRRTCFQGDRTGHEMMTALQEEVIREDIQTIGEVM
                     ITTLLRDGKGRVAGACGFSLPNTDLIVFEASSTILATGGAGWLYPVTSN"
     gene            736..>1579
                     /gene="sepS"
                     /locus_tag="PKK85_08145"
     CDS             736..>1579
                     /gene="sepS"
                     /locus_tag="PKK85_08145"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HNS26025.1"
                     /translation="MNKKEIIKLAKRDFERAWMETAHSLKKPHHDDEYPRLLLKTGKT
                     NHLYDTIWELRQAYLMLGFEETINPLFIEEDHVYRQFGPEAPAVLDRCFYMAGLPRPD
                     IGLGIDKVDKIESLGVSLDESKITGLQEVFRQYKKGETSGDDLVLDLSCALDVENEMG
                     MRILERVFPEFHELKPIASKTTLRSHMTSGWFLTLQNLHNNRSLPLKLFSIDRCFRRE
                     QKEDSSHLMTYHSASCIWMDDEISLDVGMAVSESLLKHFGFEKFRFQPDDKKSKYYIP
                     ETQTE"
BASE COUNT          446 a          376 c          332 g          425 t
ORIGIN      
        1 attggaggtt acagggtaaa gccatcccgc cccacctgta gctaaaattg ttgatgaagc
       61 ttcaaataca atcagatctg tgtttgggag tgaaaatcca catgccccgg ccacccggcc
      121 ctttccatcc ctgaggaggg tggtgatcat cacttcccct atggtctgga tgtcttccct
      181 tatgacttct tcttgtaggg cggtcatcat ttcgtgacct gtgcgatccc cctgaaaaca
      241 agtgcggcga taggtttgtc caccaaatgg tcgttggtta agcaacccag attcttgccg
      301 gtcaaaaatg gcgccaaatc cttctaattc atttaatcta tctggagctt cctcaaccaa
      361 aattttagct agttttctgt cgttaaggta gcccccaccc ctgagagtat cctgaaagtg
      421 tgcttcagta ctgtcttcct catctacata ggcaaaagca gcgttatagc ccccttccgc
      481 cagggtagtg catcctgatt tgaaggaaag tcccttcgag actattatca catcccgacc
      541 atgttgacga gcctcgatag ctgctctgca gccagctcca cctgatccaa tcaccaggac
      601 atcggtttgg tagatttccc tttccatact atgcagttga ccttagaacc tatttaattt
      661 atccccccct aaggctagaa tataaaaagg atagaaatct atttctaata tattagaaga
      721 aggggaggca gttcactgaa taaaaaagaa attataaagc tggctaaaag ggattttgaa
      781 agggcctgga tggaaacagc ccactcctta aaaaaacccc accacgatga tgaatatccc
      841 cgtttattgc tcaagacagg taaaacaaac catctctacg atactatttg ggaattaaga
      901 caggcttatc tgatgctggg ttttgaagaa accataaatc ccctttttat tgaagaagat
      961 catgtttatc gccaatttgg accggaagca cctgctgtac tagaccgttg tttctacatg
     1021 gccggactgc cccggccaga tattggactg ggaattgaca aagtagataa gattgagagc
     1081 ctgggggttt ccctagacga gtcaaagatc actggcttgc aggaagtctt ccgacaatac
     1141 aaaaaaggag aaaccagtgg ggatgatctg gtactagatc tttcctgcgc cctggatgtg
     1201 gaaaatgaga tgggaatgcg gatcttagaa cgagtattcc ctgaattcca tgaacttaaa
     1261 ccaatagcca gtaaaaccac ccttcgttcc catatgactt ccggctggtt cttgacctta
     1321 caaaatctgc ataataaccg ttccttaccc ctcaaactct tttccattga caggtgcttc
     1381 cgccgggaac aaaaagaaga ttccagtcac ttgatgacct atcattctgc ttcctgcata
     1441 tggatggatg atgaaataag cttggatgtt ggaatggctg tatctgagag ccttctgaaa
     1501 cattttggct ttgaaaagtt ccgtttccaa cctgatgata aaaaatccaa gtactacatc
     1561 cctgaaaccc agacggaag
//
LOCUS       DAOQVW010000419         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_107021_length_1579_cov_2.659449, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000419 DAOQVW010000000
VERSION     DAOQVW010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107021_length_1579_cov_2.659449"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKK85_08150"
     CDS             <1..226
                     /locus_tag="PKK85_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26026.1"
                     /translation="TNGNLIQNISIRTPSIMNIDACAKYMLQDVATVADAVATYASVD
                     PCIACTERVVIVDESGKKKDVEGIHNLELS"
     gene            233..1267
                     /locus_tag="PKK85_08155"
     CDS             233..1267
                     /locus_tag="PKK85_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS26027.1"
                     /translation="MSSVIWYLYEFARKSWAESFAGAKTNPEIMETPDRFRDFPQVIP
                     ENCIACGACTAACPAPKAIKLVRSEDNSHEEGITYPVINTRGCIRCGFCAEVCPTDPK
                     ALLCGENHLIREEFTILPVEKTFTIDDYLCIRCRKCMNTCPVEGAIFEEDNKIIIDKA
                     KCISCGECLKTCPVKGAVKGIYVFNVQEQKQIIQLVVNTLEKRIEEEEENIKQLEGGE
                     VFKVDLPIDGLVEAARNILDNDDLILDIIQKMTDRLKMRIITWDHGKCNDCRLCVKDC
                     PSGAITYTKEGGVQRNADKCLRCSVCYQTCPFGVVGYYVARFLLDEPQLEGGVIRITV
                     KASQLPQGAG"
     gene            1269..>1579
                     /locus_tag="PKK85_08160"
     CDS             1269..>1579
                     /locus_tag="PKK85_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS26028.1"
                     /translation="MAETTSPSKRIYKPLREVEVEYEIDHEKCKKCQERPCLSSCPVD
                     ALHEIPPYDQVEIDEKCVGCVLCREACPYDAIKIKTKLSEPILENIPNINTKLCIHCG
                     A"
BASE COUNT          465 a          371 c          391 g          352 t
ORIGIN      
        1 gaccaacggt aatcttattc agaatatttc tatacggact cccagcatca tgaacatcga
       61 cgcctgtgcc aagtacatgc tacaggacgt ggccactgta gccgatgccg tggcaaccta
      121 cgccagtgtg gatccctgca ttgcctgtac cgagagggtg gttatagtgg atgagtctgg
      181 taaaaagaaa gatgttgaag gtattcataa tttagaacta agttgatgtc ctatgtcatc
      241 tgtaatctgg tacctctacg aattcgcccg aaaatcctgg gctgaaagct tcgcaggggc
      301 aaaaactaat cccgagatca tggaaactcc tgatagattc cgtgacttcc cccaggtgat
      361 ccctgaaaac tgcatagcct gcggtgcctg tactgccgcc tgtccagccc caaaagccat
      421 caaactggtg cgtagtgaag ataacagtca tgaagaagga atcacctacc cggtgatcaa
      481 cacccggggc tgcatccgct gcggattctg tgccgaagta tgtcccactg accccaaggc
      541 cctgctttgt ggtgaaaatc acctcatcag ggaggagttc accatcctcc cagttgaaaa
      601 aaccttcacc atagacgatt acctctgcat ccgctgcagg aagtgtatga acacctgtcc
      661 cgtggaggga gccatatttg aagaggataa caagatcata atagacaagg cgaaatgcat
      721 cagctgcggg gaatgtttga agacctgtcc agtgaaggga gctgttaaag gcatctacgt
      781 gtttaacgta caggaacaga aacaaataat ccagctagtg gttaacaccc tggaaaagcg
      841 gattgaagag gaagaagaga atattaaaca actggaaggt ggagaggttt tcaaagtgga
      901 cctgcccata gatggactgg tagaagctgc ccgtaatata ttggacaacg atgacttgat
      961 attggacatc atccagaaaa tgactgaccg cctgaagatg cggatcataa cctgggatca
     1021 tgggaaatgt aatgattgcc gtttatgtgt caaggactgc ccatctggag ctataaccta
     1081 cactaaagag gggggagtcc agaggaatgc agataagtgc cttcggtgca gtgtctgcta
     1141 tcagacctgt cccttcggtg tggtgggtta ttacgtggcc cgcttcctgc tggacgaacc
     1201 ccaactagaa gggggagtga ttcgtataac ggtaaaggca tcccaactac ctcaaggagc
     1261 tggttaatat ggctgaaaca acgtccccat ccaaaagaat ttacaagcca ctccgggaag
     1321 tggaggtgga atacgaaatt gaccatgaaa aatgtaaaaa atgccaggaa cgtccctgcc
     1381 tatcctcctg tcccgtggat gccctgcacg aaataccacc ctacgaccag gtggaaattg
     1441 atgaaaaatg tgtgggttgc gttctatgcc gggaagcatg tccatatgat gcaattaaaa
     1501 taaaaaccaa attatcagag cctatcctgg agaacatccc caacatcaac accaaactct
     1561 gcatccactg cggagcctg
//
LOCUS       DAOQVW010000420         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_107110_length_1578_cov_3.355220, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000420 DAOQVW010000000
VERSION     DAOQVW010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107110_length_1578_cov_3.355220"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1227)
                     /locus_tag="PKK85_08165"
                     /pseudo
     CDS             complement(34..1227)
                     /locus_tag="PKK85_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
     gene            complement(1303..>1578)
                     /locus_tag="PKK85_08170"
     CDS             complement(1303..>1578)
                     /locus_tag="PKK85_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HNS26029.1"
                     /translation="VYSLDPTGGVVKDMVISTGSGSPFAYGVLEDRYSEDLYVDEGVD
                     VAIRAIKAAMERDTFSGNGIVVATITAEEGFKKLNEEEVEKKIKQIS"
BASE COUNT          409 a          350 c          337 g          482 t
ORIGIN      
        1 aaaaaaacgt ggagcgaaat ataacagcta ctcttttatc agcctttttt cagcatcgtt
       61 tcgggtgggc tgcacgtcat catggccacc gtatgtgctc tccattacca atgattctat
      121 acgtgggaac ttggatactg ccggttccag aagccggctt ctttcaaatt tgaaatctcc
      181 ggtgtagaca aaattatgct gaccatcccc tatgtgcatg tgggtgatgg cagatcccag
      241 tatatgcccg gcattgtgca atgttaagcg aatatccggg gcgatatccg taacttcacc
      301 gtaatccagg gttatagtat gtttaatgca ttttttaaca tgtttaacgt tgaagggcag
      361 tggactatct tctcgatggg ctatatcaat atgatcaagt tggagaagag tcatcagatc
      421 acgggtgggg gtggtgcagt atactggacc ctcgtaaccg taatggtaaa gataagggag
      481 gaatcctgag tgatccaagt gggcgtgtga tatgatcacc gcatccaagt catctaacac
      541 aaattctgga acgttcaagt aaggataaga acttttatcg tctactcctg caacgttaac
      601 tccgcaatca agtaatattt tgctgttgct ggtttgtaag aataatgaag aacgacctac
      661 ctctctgaaa cctcctaagg cagttatacg tgcccattca ttttccatat aaactgggcg
      721 gtgtatgcga ttacccagtt cctgcagaat ctttttcctc tctttactat tttgtctgag
      781 agttcttctg atcctttgga taacttcaga ggatataggg ggtgttctaa gaattttcgg
      841 cgcccaaccg atctgcttaa cgatttctct ggatgtagct ccgtacttac ctataaccag
      901 ccctggtttt ctggcttcaa tgataacttc gcatgtcact tcatcgaagg atatgttggt
      961 tatcttggcc tcctggggaa cgatctggtg gatgtggtcg attgaagtct caggctccat
     1021 gagcacagat ttgtccgagc ggatgatgat gcgtttccgg aggtccttgg ccagatctcg
     1081 gataagatca ccattttcag taatgatctc cggatttttg gtgtatataa ccacctccgg
     1141 tccctcaaat tccactttgg ctacttgcac tctattgggc aatctttgta ctatggtatt
     1201 tttgatttct tgaatctctg aacccataac atcacgtcta gaaaaataga aataaaatcc
     1261 ggaccttttt ggtcctatga aaaatttaat aaaaagtaga acttagctga tttgtttaat
     1321 ctttttttct acttcttctt catttagttt tttaaagccc tcttcagcgg tgatggtggc
     1381 caccacgata ccattcccgg aaaaggtgtc acgttccatg gcggccttta tagcacgaat
     1441 agcaacatca acaccttcat ccacgtagag gtcttcactg taacggtctt cgaggacccc
     1501 ataggcaaat ggcgaacctg aaccagtaga tatcaccata tccttaacta cccctccagt
     1561 gggatccaga gaataaac
//
LOCUS       DAOQVW010000421         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_108919_length_1563_cov_1.568302, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000421 DAOQVW010000000
VERSION     DAOQVW010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108919_length_1563_cov_1.568302"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1129
                     /gene="sucD"
                     /locus_tag="PKK85_08175"
     CDS             269..1129
                     /gene="sucD"
                     /locus_tag="PKK85_08175"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNS26030.1"
                     /translation="MIILDKNTRCIVQGITGKQGTFHTQGMLDYKTNIVAGTTPGKGG
                     QKFGEIDVYNSIAEAKNELDINASIIFVPAPFAKDAAFEAISQLDLVVIITEHIPVHD
                     TIEVVEYAHRHDTIVIGPNTPGIITPGVGKLGIMPTHIFQEGEVGIISRSGTLTYEVA
                     HQITRAGLGQSTCLGIGGDPVVGMDFSRVLQMFEEDKYTKAMAMIGEIGGNAEEKAAE
                     YIRENISKPVVAYIAGVTAPPGKRMGHAGAIIEGESGTAKSKIETLEKAGVQVASRPS
                     QIAEIMKDIL"
     gene            1250..>1563
                     /locus_tag="PKK85_08180"
     CDS             1250..>1563
                     /locus_tag="PKK85_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HNS26031.1"
                     /translation="MVEKEEIINKLLNGELKLYEIDKFTENTREAVDIRRKFLEEKYK
                     IQLTNIANYSLDMDEAQKRNIENPIGTIQIPVGLAGPLKINGLNTQGKFYVPLATSEG
                     ALV"
BASE COUNT          520 a          341 c          369 g          333 t
ORIGIN      
        1 aggctcacca tcctatgtga tacccggtag tgttatcatg tccatcaccg catacatgaa
       61 cggcaacgtc atggacctgg cagttcccct ctctgagagg atgcgtattt tgaaatagtg
      121 gtcatggaaa tggtggtaag taaaatttaa aatggtgacg aagcaaatag caaatcaccc
      181 ctttttatta ccaacaaagg actatcataa ttacagtacc acacataaaa catggtttaa
      241 atgaagagaa gtaggggttt gaaggaagat gataattcta gataaaaaca cgagatgcat
      301 agtacagggc atcaccggaa aacaggggac cttccacacc cagggaatgc tggactacaa
      361 aactaacatt gtagccggaa ccacccccgg aaagggaggg cagaaattcg gtgaaataga
      421 tgtttacaac tccatagcag aggcaaaaaa tgaattagat atcaacgcat ccataatatt
      481 tgtaccagca ccctttgcca aggacgctgc ctttgaagcg atatcacagc tcgacctggt
      541 ggtcatcatc accgagcaca tcccggtaca cgataccata gaggtggtgg aatacgccca
      601 caggcacgac accatagtca tcggccccaa caccccggga atcatcaccc ctggagtggg
      661 gaagttagga ataatgccca cccacatatt ccaggaggga gaagtgggca taatctcccg
      721 gagcgggacc cttacctacg aggtggcaca ccagatcacc agggccggct taggccagag
      781 cacctgcctg ggaataggcg gagacccagt ggtaggaatg gacttctcac gcgtactgca
      841 gatgtttgaa gaagacaaat acactaaggc catggccatg atcggagaga tcgggggaaa
      901 cgccgaggaa aaagccgctg agtatattcg ggaaaacatc agcaaacccg tagtggcgta
      961 catcgccggg gtaaccgccc cgccgggtaa gaggatggga catgccggtg ccattataga
     1021 aggtgaaagc gggactgcca aaagtaaaat agaaaccctg gagaaagccg gggtgcaggt
     1081 tgcaagcagg ccttctcaga tagccgaaat aatgaaggac attttataac tttttttaat
     1141 atatgaattt tcctccctca attggttaaa atataaattt tatgaattca aaaaattata
     1201 taaactcaat tttattaaat tctctaaata tttcaggatc tgtgagaaaa tggtcgaaaa
     1261 ggaagaaata ataaataaac ttctaaatgg tgaacttaaa ctatacgaaa ttgacaaatt
     1321 caccgagaac acccgggaag ctgttgatat aaggcggaaa tttttagaag agaaatataa
     1381 gatccagtta accaacatcg caaactactc cctggatatg gatgaagccc agaagagaaa
     1441 catagaaaac cctatcggaa ctatccaaat tccagttggc ctggccgggc cgctaaagat
     1501 caacggttta aatactcagg ggaagtttta cgttccactg gccacctcag aaggggctct
     1561 ggt
//
LOCUS       DAOQVW010000422         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_110303_length_1551_cov_2.784091, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000422 DAOQVW010000000
VERSION     DAOQVW010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110303_length_1551_cov_2.784091"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKK85_08185"
     CDS             complement(<1..404)
                     /locus_tag="PKK85_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS26032.1"
                     /translation="MKIIPLAFESFGVRSMATYVETDQKILIDPGTSLGPKRFGFPPW
                     KTELEALYHTRAQIQEHAGKADIVTISHYHHDHFTPFQVGKYLDSNPQYAAQIYHDKQ
                     LFIKDPTNKINQNQKKRARSLLKNLVNSKISYA"
     gene            703..954
                     /locus_tag="PKK85_08190"
     CDS             703..954
                     /locus_tag="PKK85_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26033.1"
                     /translation="MLSNPPHWDLGNPSQLSLAGPGRNSPMSGALHQQLVNPSLPIKG
                     NVVKNLTIHKHILEGIVQVLPFPESNIIQFRPEKRPFSG"
     gene            complement(983..1108)
                     /locus_tag="PKK85_08195"
     CDS             complement(983..1108)
                     /locus_tag="PKK85_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26034.1"
                     /translation="MNQFLARKIEPEHVFFGSSGVNVASLEGTEHYFRWEPFQEL"
     gene            complement(1358..1495)
                     /locus_tag="PKK85_08200"
     CDS             complement(1358..1495)
                     /locus_tag="PKK85_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNS26035.1"
                     /translation="MAKKSVYIRLEPEYVQRIDELAQKQDRSRSYIMRQLVIKGLGKM
                     K"
BASE COUNT          431 a          323 c          336 g          461 t
ORIGIN      
        1 gcgtaggata tcttactgtt caccaggttc ttcaggagac ttcgggccct ttttttctga
       61 ttctggttaa tcttgttggt gggatctttt atgaacagtt gcttgtcatg gtagatctgg
      121 gccgcgtatt gggggttgga gtccagatac tttcccacct ggaagggtgt gaaatgatca
      181 tggtggtagt ggctgatggt cacgatatcg gccttcccag cgtgttcctg tatctgggcc
      241 cgggtatggt acaatgcttc taactcggtc ttccaggggg gaaatccaaa ccttttgggc
      301 cctaaggatg ttccggggtc gatgagtatc ttctgatccg tttccacata ggtggccatg
      361 gaacgaacac cgaagctctc aaaggccagg ggaattattt tcacttggat caccatttat
      421 gtttaggttt ggtaaccaaa tataatctta cactcacatc ctgaaaatag aggcctgcaa
      481 gctaaataat tgaatgatga aggatttatt taatgattaa aaaccgggca aagggaatta
      541 ctgtaacggt gcatgggata ttatcgactc ggggatctgc cagtgattcg aagctaaaat
      601 cttttttaaa aaaacatttc cacccataat ttattacgag gatgtaaaga agtgcatttt
      661 agataataac cttacttata ttgtaccagg ggttatcatg gaatgttgtc caatccaccc
      721 cactgggatc ttggaaatcc aagtcagcta agtcttgcag ggcctggtag gaattctcct
      781 atgtctggag cgttacacca gcaactggtc aatccatccc taccaattaa aggcaatgtg
      841 gttaagaatt tgaccatcca caaacatatc ctcgaaggaa tagtacaggt tttaccattc
      901 ccagaatcca atatcatcca gttcagacca gaaaagagac cattctccgg gtaaagattc
      961 tatactaact cctcggaagg cttcataatt cttgaaaggg ttcccaccgg aaatagtgtt
     1021 cagttccttc cagggacgct acgttcacac cactggaacc aaaaaatacg tgttcgggtt
     1081 cgattttacg ggctaggaat tggttcatgt ggggtgtcac gttaccatga agattgaaaa
     1141 ccaatctaaa tgggacagct tcaatctcat ttactggggg actgtaggta aaaaccagtt
     1201 tccaggggct tggcatatac tttactcttt atttctccca taaaggtggg atacaccata
     1261 ggggcttagt aaaaatgcta aaagtaatac tagttatgcc tgtttcccac ataaatcagt
     1321 gactagaaac taaatattat taaatactta taaagtatta cttcatcttt cccagccctt
     1381 ttattactaa ttgccgcatt atgtatgatc tggaacggtc ctgcttctgg gccagctcat
     1441 ctatcctttg cacgtactcc ggctccagtc ggatgtagac actcttcttc gccatgtata
     1501 tagttacgat tcagtacata taaatacatt acagacgaag tggattaaat g
//
LOCUS       DAOQVW010000423         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_112605_length_1532_cov_1.633040, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000423 DAOQVW010000000
VERSION     DAOQVW010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112605_length_1532_cov_1.633040"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKK85_08205"
     CDS             complement(<1..665)
                     /locus_tag="PKK85_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26036.1"
                     /translation="MKDHEDSPEKTLQEKNHHPQEENNHSIEHEHREHPEQGHSKHGE
                     HKENKRTEHEGHTEHLKQDHTKHEEHVEHEHKEHLEHDEHPEHNHHDHHQAMMADFKK
                     RFIISLIITVPVFFLSEMVQELLGLHFLSFQGDIYILFLLSSFVFFYGGYPFFTGMVG
                     ELKVRQPGMMTLVAVAITTAYIYSAGVTFGLMGMTFYLELVTLIDIMLLGHWIEMRSV
                     MGAS"
     gene            complement(813..>1532)
                     /locus_tag="PKK85_08210"
     CDS             complement(813..>1532)
                     /locus_tag="PKK85_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS26037.1"
                     /translation="VGIVFQNPDDQLFAPTVIEDVAFGPMNLGLSEDEVDGRVEEALK
                     LVGMTGLEKKAPHHLSGGQKKRVAIAGILAMRPEIMVLDEPTTGLDPTGVDQIMKILY
                     MLNKEAMSIIIASHDVEMVTQFADKIFVLHDGEIIGQGTTEEIFNDCETLYKAHLRPP
                     KSAELLYMLKNEGFPVNIKLTVDEAYHEILHALGSQSYHNLLHLVKDDLHHKLLHSLG
                     EDKYHELLHLLEENQKDVTSS"
BASE COUNT          384 a          323 c          330 g          495 t
ORIGIN      
        1 gaggcgccca tcacagagcg catttctatc cagtgcccca gtagcatgat atcgattaga
       61 gtgaccagtt ccaggtagaa ggtcataccc ataagtccga aggtaactcc cgcactgtag
      121 atgtaggctg tggtgatggc caccgctacc agggtcatca tacctggctg cctgactttc
      181 aactcaccta ccatgccagt gaagaagggg taacccccat agaagaatac aaaggatgat
      241 aatagaaaca ggatgtaaat atcaccctgg aaactgagaa agtgcagtcc cagtaattcc
      301 tgcaccatct ctgagaggaa aaagacaggg acggttataa tcagagatat tatgaatctt
      361 ttcttaaaat cagccatcat ggcctggtga tggtcgtgat ggttatgttc tgggtgttca
      421 tcgtgttcca gatgctcttt atgctcatgt tctacatgct cttcgtgttt tgtgtggtcc
      481 tgttttaaat gttcagtgtg tccttcgtgt tctgtacgtt tgttttcttt gtgttctccg
      541 tgtttggagt ggccctgttc aggatgttct ctatgttcgt gttctattga gtggttattt
      601 tcttcttggg ggtggtgatt tttttcttga agtgtttttt caggggaatc ttcatgatct
      661 ttcaaggata tcatccctta aactgtaaaa aattcgcaac ttcatccaat ttgtaacctg
      721 atatttgtat caaacagttt aattttttaa actcaaacag tcaatgtatt aaaatatgta
      781 cttgctttct taaattatag tagggtaaat cgctaggaag atgttacatc cttttgattc
      841 tcttctagca agtgtaaaag ttcgtgatat ttatcttctc ccaggctatg caggagttta
      901 tgatgtagat catctttcac cagatgaagc agattatgat aggactgact tcctaaggca
      961 tgcaggatct cgtgatatgc ttcatcaaca gttaacttaa tgttaacagg gaaaccttca
     1021 tttttaagca tatatagaag ttctgctgat ttgggtgggc gcagatgggc tttataaagg
     1081 gtttcgcaat cattgaatat ctcttcggtt gtgccttgac cgattatctc accatcgtga
     1141 agtacgaaga tcttatctgc aaattgagtg accatctcca catcatggga agcgatgatg
     1201 atgctcatag cttccttatt taacatgtaa agtattttca ttatttgatc cacacctgta
     1261 ggatctagtc ccgtggttgg ttcatccagt accatgatct caggtctcat ggccaggata
     1321 ccggcaatgg ctactcgttt tttctgtcca ccactcaagt ggtggggtgc ttttttctcc
     1381 agcccagtca tccccaccag tttcagggct tcttccaccc gtccatccac ctcatcctca
     1441 gaaagtccca ggttcatggg tccgaaggcc acgtcctcaa taacagtagg tgcaaagagt
     1501 tgatcatctg ggttctggaa gacgatacct ac
//
LOCUS       DAOQVW010000424         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_112741_length_1531_cov_1.247290, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000424 DAOQVW010000000
VERSION     DAOQVW010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112741_length_1531_cov_1.247290"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /gene="rpsB"
                     /locus_tag="PKK85_08215"
     CDS             <1..439
                     /gene="rpsB"
                     /locus_tag="PKK85_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNS26038.1"
                     /translation="AAKFLAKYDPDDILVVSTRQYGQAPVKRFGEIVGAKTIPGRFIP
                     GTLTNPEYAKFIEPKVLVVTDPRSDSQAIIEAKQMGLPVVALCDTENLLGNVDIVVPV
                     NNKGRKAIALVYWLLARQILREKGIIAEDADLDVEPTDFELKI"
     gene            571..1425
                     /gene="amrB"
                     /locus_tag="PKK85_08220"
     CDS             571..1425
                     /gene="amrB"
                     /locus_tag="PKK85_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNS26039.1"
                     /translation="MIKNPAVAGTFYEADPDSLTKQIEGCFKHQVGPGRLPENMGKTR
                     EIKSIIAPHAGYMYSGPVAAHSYLKLAEDGLPETFLILCPNHTGMGSGVSTVTEGGWK
                     TPLGLVDIDGKFAERLMENAPIIDPEPLAHIQEHSCEVQVPFIQYLAEKAGKTFRIVP
                     ICMWMQDLQTATEIGAAIAKTSQELLRDVVIVASTDFTHYKPHDIAYHNDHQVLDAIE
                     ALDEKAVMSRVEELNVTMCGFGPVNASLVYSKLMGAEKAEVLKYGTSGDITGDRSAVV
                     GYAAVVIR"
BASE COUNT          509 a          336 c          361 g          325 t
ORIGIN      
        1 agcggccaaa ttcctggcca aatacgatcc agatgatata ctggtagtgt caaccagaca
       61 gtacggacag gcaccagtca aaagatttgg agagattgta ggagcaaaga ccatacctgg
      121 aagattcata ccaggaacct tgaccaaccc agaatatgcc aagttcatag aacccaaagt
      181 actggtggta accgaccccc gatcagactc ccaggccata atagaagcca aacagatggg
      241 cctgcctgta gtagcgctct gtgatacaga aaacctcctg ggaaatgtgg acatagttgt
      301 accagtaaac aacaagggaa gaaaggcaat cgcactggtc tactggttac tcgccagaca
      361 gatcctccgg gagaaaggaa tcatcgccga agacgccgat ctggacgtgg aaccaacaga
      421 tttcgaattg aagatataaa ctggtttaat ctataaaaaa atcaagatct taaaaatttt
      481 aagggattat attagggatt caataggaaa tagtaggatc aagtagaaat tgaggatata
      541 ataaaaaaaa ttaaagaggc agtgatttaa atgataaaaa accctgctgt agcaggtact
      601 ttctacgaag cggatcctga ttctttaact aaacagatag aaggttgttt taaacaccag
      661 gttggcccgg gaagactacc agaaaacatg ggaaaaacac gtgagataaa gagtatcatc
      721 gcaccacatg ccgggtacat gtactccgga ccagtggccg cccactccta tctgaaactt
      781 gcagaagacg gactgccaga aacattcctc atactatgcc ccaaccacac tggaatggga
      841 tccggtgtct ctacagtaac cgagggcggg tggaaaaccc cactcggcct cgttgatatc
      901 gacggaaaat ttgcagaaag gttgatggaa aacgcaccca tcatagaccc agaaccactg
      961 gcccacatcc aggaacacag ctgcgaagtc caggtaccct tcatacagta cctggcagag
     1021 aaagctggta aaacattccg gattgtgccc atctgcatgt ggatgcagga tctacaaact
     1081 gcaacggaga taggagctgc catagcaaag accagccagg agcttctaag agacgtggtt
     1141 atagtggcca gcacagactt cacccactac aaaccccatg atatagccta ccacaacgac
     1201 caccaggtcc tagatgcaat agaggccctg gatgagaagg cggttatgag tcgggtggag
     1261 gaacttaacg ttaccatgtg tggcttcgga ccggttaacg catccctggt ctattccaag
     1321 cttatgggtg cagaaaaggc agaagtactt aagtatggga ccagtggtga tatcactgga
     1381 gatagaagcg cagtggtcgg ttatgcagcg gtagttatcc ggtaagaaag tttccaaagg
     1441 tctcatgaat ttagaaagat tctaaactga ataacttact taattaaaga agtcttactc
     1501 aattaaaatt cctaaaatgg gtgattctaa t
//
LOCUS       DAOQVW010000425         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_113383_length_1525_cov_3.657823, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000425 DAOQVW010000000
VERSION     DAOQVW010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113383_length_1525_cov_3.657823"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..832)
                     /gene="rrp41"
                     /locus_tag="PKK85_08225"
     CDS             complement(95..832)
                     /gene="rrp41"
                     /locus_tag="PKK85_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HNS26040.1"
                     /translation="MINIITNRDLEVKSEAKGSERPDGRAYNELRPLKIEAGVLERAD
                     GSAYLEFGDNKVLAAVYGPREFHIRRFLKPNMAVLRCRYNMAPFSVEDRKRPGPDRRS
                     LEISKLTSEALSPAVFLEKFPRSSIDVFIEVLQAEGGTRCAGITAASVALADAGIPMR
                     DMVVACAAGKANGQIVMDLSEWEDKEGEADVPVAIMPRTGEITLLQMDGQLTKDEFEQ
                     ALDLAKEGCMKISVEQKKAIKNRYGGD"
     gene            complement(836..>1525)
                     /gene="rrp4"
                     /locus_tag="PKK85_08230"
     CDS             complement(836..>1525)
                     /gene="rrp4"
                     /locus_tag="PKK85_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HNS26041.1"
                     /translation="GEVLAEGEYYAGRGTFKDDTKVCSALVGLVAIRDDKISVLPLQS
                     KYIPKRGDVVIGKITEVRFSMWNVDINSPYSGILPASDVFGREKRDMNKAFNVGDILF
                     MRVVDVDEVKKVKLGLKGRGLGKFRGGILVEITPTKVPRLIGKKGSMINMIKDHTKCE
                     VVVGQNGLVWVKGKPEMERVVEKVIKTIETEAHTSGLTDRIRVMLNQLLDIEEDVNKE
                     EEEDYKEELIQ"
BASE COUNT          353 a          409 c          271 g          492 t
ORIGIN      
        1 gtactggtcc aatgatcgac catcggttct ttcacccttg tttatgaggt tggttacact
       61 aattctggta atttcaggaa ctatgggctg catcttaatc accaccatac ctgttcttta
      121 tggccttttt ctgttctacg ctaattttca tacagccttc tttggccaag tccagggctt
      181 gttcaaactc atctttcgtt agttggccat ccatctgtag taaagtgatt tcaccggtac
      241 ggggcataat ggccactgga acatcggctt caccttcctt atcctcccat tcagaaagat
      301 ccataactat ctggccgttg gcctttccag cggcacatgc caccaccata tctctcattg
      361 gtatacctgc atctgccaag gccaccgacg ctgctgttat ccctgcacag cgggtgcctc
      421 cttctgcttg gagaacctca atgaatacgt ctatagatga tctgggaaat ttttccagga
      481 aaactgctgg ggataatgct tctgaagtta atttggatat ttctagtgat ctacggtctg
      541 gacctggcct tttcctgtct tctactgaaa aaggagccat gttataccta catcgcagta
      601 ctgccatgtt aggttttaaa aaccggcgta tatgaaattc tcgtggcccg tatacagccg
      661 ctaaaacttt gttgtcaccg aactcaagat aagcagaacc atctgctctt tctaagactc
      721 cagcctctat ttttaagggc cttaactcat tataagctct accatccggc ctctcagagc
      781 cttttgcttc gctttttact tctaagtcgc gattggttat aatattaatc accccttatt
      841 gaattaattc ctctttgtaa tcttcctctt cttctttgtt tacgtcttcc tcaatatcca
      901 ggagctgatt taacattact cgaatacgat cagttaagcc agaagtgtga gcttctgttt
      961 caatggtttt tattaccttt tccaccactc tttccatttc tggttttcct tttacccata
     1021 ccagaccatt ctggcccacg accacttcac atttagtgtg gtctttgatc atgttaatca
     1081 tggatccctt cttacctata aggcggggta ccttggtggg agtaatttcc accagtattc
     1141 ctccccggaa tttacctaga ccgcggcctt taaggcccaa tttaaccttt ttaacctcat
     1201 ccacatccac cacacgcata aaaagtatgt cacctacatt gaaggccttg ttcatatctc
     1261 ttttttccct tccgaacacg tcggatgcag gtaggattcc ggaataggga gagttaatat
     1321 ccacattcca catggagaat ctgacctcgg tgatctttcc aataaccaca tcccccctct
     1381 tgggaatgta cttgctttgc aggggcagta cacttatttt atcatcacgt atggccacca
     1441 gccccaccag ggctgaacag actttagtgt catccttaaa agtcccccta cctgcgtagt
     1501 attctccttc tgccagaacc tcccc
//
LOCUS       DAOQVW010000426         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_113551_length_1524_cov_2.462900, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000426 DAOQVW010000000
VERSION     DAOQVW010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113551_length_1524_cov_2.462900"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..357
                     /gene="mtrB"
                     /locus_tag="PKK85_08235"
                     /pseudo
     CDS             56..357
                     /gene="mtrB"
                     /locus_tag="PKK85_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit B"
     gene            370..1092
                     /gene="mtrA"
                     /locus_tag="PKK85_08240"
     CDS             370..1092
                     /gene="mtrA"
                     /locus_tag="PKK85_08240"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HNS26042.1"
                     /translation="MADKKAPAEGWPVINGDYVVGDPESPVAATTLASHIEQIPVDAG
                     AAIAGPCKTENLGIEKMLANLISNPNIRFLVLCGSEVQGHITGQSIEALHANGVDPDK
                     RKIIDATGAIPFIENIPDEGIERFQQQMEIISIIDVEDAAAIQSKIKECIEKDPGAFE
                     EEAMVIEVEEGGGEEEEGEEIPPVTPETALIDARMRSIQTQVKSIGSLQRISAGMYSG
                     KVQGIMIGMIFILTLGTILLLQ"
     gene            1104..1313
                     /gene="mtrF"
                     /locus_tag="PKK85_08245"
     CDS             1104..1313
                     /gene="mtrF"
                     /locus_tag="PKK85_08245"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit F"
                     /protein_id="HNS26043.1"
                     /translation="MLISNKPNIRGIRKVAADVKYRTQLIGRDQRLFSGLIATRIYGM
                     ALGFILALLLVGIPVIWVLMSVSGA"
     gene            1318..>1524
                     /gene="mtrG"
                     /locus_tag="PKK85_08250"
     CDS             1318..>1524
                     /gene="mtrG"
                     /locus_tag="PKK85_08250"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit G"
                     /protein_id="HNS26044.1"
                     /translation="MAEEEKTVIARPIVPADEFNKANDRLDEMDEKVEFTWGEISQRM
                     GQQIGRDIGILYGIVIGLIILFVAF"
BASE COUNT          482 a          299 c          373 g          370 t
ORIGIN      
        1 ctgtccaatg gtctggtatg tggcctaaag aggaggaaca ctaggaggaa tagaaatgga
       61 aatgttacca ttagtcaaag tagttcctga aatgaaccta actttagacc ccaccactgg
      121 tatgataggg gcggccttag gcgtgatgtc gttatcgtat ctatggacgg agtaaacgaa
      181 caattagatg tattagaggt tgcagtagaa gacttataca cagccctgga tccaactaca
      241 gtgtctgaag gttcttaccc tggaagggaa ggtgtgtacc ttaccgctgg taagctaacc
      301 aacatggttt acggattcat cttagggtta gtactactgc tggccctatt gttataggag
      361 gtgttaaagt tggctgataa gaaagcacca gcagaaggat ggcctgtaat aaacggggac
      421 tacgtagtag gtgacccaga aagccctgtt gcagctacta cactggcttc gcacatagaa
      481 caaataccag tagacgccgg agctgcaatc gccgggccct gtaaaactga aaacctgggg
      541 atcgagaaaa tgctcgccaa cctaatttca aaccccaaca tcagattcct ggtactgtgc
      601 ggatctgaag tgcaaggcca catcacagga caaagtatag aagcactcca cgccaacggt
      661 gttgaccccg ataaaaggaa aataatcgat gccaccggag caattccctt catcgagaac
      721 attcccgacg aaggaatcga aagattccag caacaaatgg aaataatcag catcatcgac
      781 gtggaagacg ccgcagctat acaatccaaa atcaaagaat gtatcgaaaa agaccccggt
      841 gcattcgaag aagaagcaat ggtcatagaa gtcgaagagg gtggcggaga agaggaagaa
      901 ggtgaagaaa taccaccagt aactcctgaa actgctttaa tagatgcaag aatgcgtagt
      961 atccaaaccc aagtaaaatc cattggtagt ttgcagagga tatccgcagg tatgtattct
     1021 ggtaaagtcc agggaatcat gattggtatg atcttcatcc tgactcttgg aaccatactg
     1081 ctcttacaat agggggtttt tagatgttaa tatcaaataa acctaatatt agaggcatac
     1141 gtaaagtagc agcagatgta aagtaccgaa cccaactgat tggtagagat cagagactgt
     1201 tttctggtct cattgccacc cgaatttacg gtatggctct gggattcatc ctagcacttc
     1261 tcttggtagg tattccagtc atatgggttt taatgtctgt tagtggggct tgataaaatg
     1321 gctgaagaag aaaaaactgt tattgcacgt cccatagttc ccgcagatga gttcaacaag
     1381 gccaatgatc gattagacga gatggatgaa aaggttgagt tcacctgggg tgaaatatct
     1441 cagagaatgg gtcagcaaat aggcagggat attggtattt tatatggaat tgtaatagga
     1501 ctaataatcc tgtttgttgc tttc
//
LOCUS       DAOQVW010000427         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_113777_length_1522_cov_3.277437, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000427 DAOQVW010000000
VERSION     DAOQVW010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113777_length_1522_cov_3.277437"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKK85_08255"
     CDS             complement(<1..626)
                     /locus_tag="PKK85_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS26045.1"
                     /translation="MEILTHVGGTPGKDCGGFCRFCYFKTVNFKDLDSVALGCKFCPP
                     HQIGCSYCHELIREVKNGFKPPNEVLTQLIMNLEQLKNTQQEVHDLKINVGSWADVIN
                     YPHLHDLLAVFQKYEFPVNLGYTSGKGINGAETADDLVARGVTEVSFSVFSINPDLRR
                     EWMRDKTPEKSLEALQRFCELCEVNASTVVVPGVITEEEITKTCSILE"
     gene            complement(636..1037)
                     /locus_tag="PKK85_08260"
     CDS             complement(636..1037)
                     /locus_tag="PKK85_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26046.1"
                     /translation="MNKSTWIGIVGGLSIFVIILWAIIFYNSDFDQELIQSSEAGVPC
                     EKLINQIDKESLNQKSIAENHLEGHFEDIKYWNDPNDPEEELKDELFFYQQQIKAINN
                     CTELRKKYVRKEISKEEFLAQIQQYKIMMSD"
     gene            complement(1034..>1522)
                     /locus_tag="PKK85_08265"
     CDS             complement(1034..>1522)
                     /locus_tag="PKK85_08265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNS26047.1"
                     /translation="HQKNIIHGDIKTSNIMIKNGVYKISDWGLSKITTDGSVTLSGAT
                     PQYAAPEQISREFGRSDERTDIYQLGTVFYELLTGKVPFEGEMSEIYGSILTSQATPP
                     SEINPESAALEPIVMKCLSKVKDERYSSMIELQEDLEEYYTPINMDETLVFKKEPDNG
                     RS"
BASE COUNT          361 a          346 c          309 g          506 t
ORIGIN      
        1 tcctccagga tgctacaggt ctttgtgatc tcctcctcgg tgatcacccc tggcaccacc
       61 accgtggagg cgttaacctc gcacaactca cagaacctct gcagggcttc taaggatttt
      121 tcaggagttt tatccctcat ccactctctt cttaaatctg gatttatgga gaaaaccgaa
      181 aaactaactt cagttacacc cctagcaact aggtcatcgg cagtttcagc cccatttatg
      241 cccttgccac tggtgtagcc cagattcacc ggaaattcat acttctggaa gacagcgagg
      301 aggtcatgga ggtgggggta gtttatgaca tcggcccagc tcccaacgtt tatcttcagg
      361 tcatgaacct cctgctgggt gtttttcagc tgctccaggt tcattattaa ttgagttaaa
      421 acttcgttgg gaggtttaaa cccgttttta acttctcgga taagttcatg gcagtagcta
      481 caccctatct gatggggtgg gcagaattta catcccaggg ccactgaatc caggtccttg
      541 aagttcacgg ttttaaagta gcagaacctg cagaagccac cgcagtcctt tcctggtgtt
      601 ccccccacgt gggttaatat ttccatttta gtctcctaat cgctcatcat aatcttgtac
      661 tgctgaatct gggccagaaa ttcttctttt gatatttcct ttctcacata cttttttcgc
      721 agttctgtac agttattgat ggcttttatt tgttgctgat agaaaaataa ctcgtctttt
      781 agttcttctt ctggatcatt aggatcattc cagtatttga tgtcttcaaa atggccttct
      841 aaatggtttt cagctattga tttttgattt agagactctt tatcgatttg atttattaat
      901 ttttcacaag ggactcctgc ttcgctggat tgtattagtt cctggtcaaa gtctgagttg
      961 tagaaaatga ttgcccatag gataatgacg aaaatgctta aaccgcctac aatacctatc
     1021 catgtcgatt tattcatgat ctcccattat caggctcttt tttaaatacg agggtctcgt
     1081 ccatattaat aggggtgtaa tactcctcca ggtcttcttg tagttcaatc atggaggaat
     1141 atctttcatc tttaaccttg ctcagacact tcatgactat gggttccagt gctgctgatt
     1201 caggattaat ctcactggga ggtgtggctt ggctagtaag tatggatccg tatatctcgg
     1261 acatttcacc ctcaaaggga actttgcccg ttaataattc atagaatacg gttcctaact
     1321 gataaatatc ggttctttca tcggatcttc caaattccct tgatatctgt tctggagcag
     1381 cgtactgggg ggtagcacca gataaagtca cggatccatc cgtggtgatc ttgctcagac
     1441 cccaatcgga tatcttgtac actccatttt tgatcatgat gttggaagtc ttgatatctc
     1501 cgtggataat gttcttctgg tg
//
LOCUS       DAOQVW010000428         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_113965_length_1521_cov_2.075716, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000428 DAOQVW010000000
VERSION     DAOQVW010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113965_length_1521_cov_2.075716"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKK85_08270"
     CDS             complement(<1..382)
                     /locus_tag="PKK85_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26048.1"
                     /translation="MDKNATEPRLTFVPSGKVVRLCEQLFLAAWISFNSENKIHVLCF
                     MLVRKKRQSLLEKFLRNTRTPFIPEFRLKGSKFRYDIYIPQINTCIDIDSTWYHKSCW
                     SKYRSHIKREIANSSGFTTIHIKDD"
     gene            1323..>1521
                     /locus_tag="PKK85_08275"
     CDS             1323..>1521
                     /locus_tag="PKK85_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26049.1"
                     /translation="MTKKKKIVITKITLVVPARHNYETIFKLEKYYTRSGMIGITPTP
                     IEIMLKNLDRYSFIPSVGLVLL"
BASE COUNT          476 a          299 c          301 g          445 t
ORIGIN      
        1 agtcatcttt aatgtggatg gttgtgaatc cactgctgtt tgctatctcc ctttttatgt
       61 gggatctata ctttgaccag caggatttat ggtaccaggt cgaatctatg tctatgcagg
      121 tgtttatttg tggaatatag atgtcatacc ggaattttga accctttaat ctgaactcag
      181 gtatgaatgg tgttctagtg ttccttaaaa acttctccag taaagattgt ctttttttcc
      241 taacaagcat gaaacataga acatgaatct tgttctcaga attgaaggaa atccaagcag
      301 ctaggaatag ttgttcacaa agtcttacga ctttacctga tgggacgaag gttaatcgag
      361 gttcagttgc atttttatcc aactccagat ttaacagaag gaactcagtg ctcagatact
      421 gggcaagtta ctagtacttt gaaactagag gtctgttgca ggcagatact atatggtgga
      481 tttcagacat aactgctaga acagcgagga agaagggcgg gataattatc tcagtggcct
      541 aatgtctagt atcctagtag cgttagcaag aacccacgtg ccccggaatc ggtgactgtg
      601 taatagagta aatcggaagc gaaatttaac ttttgaagtt catccgttta attatgggtg
      661 tgtgaggcct tgatgctgtt gacaccgaaa agccttcaaa gggaaaaaat aaccgtatct
      721 ttacattttc aggccagccc tattaaaaat agggtgactt cgccagtcgt catagattgt
      781 tgccatgaaa ccagagaata ggggattatt catttaatct cactcggcat aaaccaccgg
      841 gaactccgtg accaacaaca gcccccaata accggggtgc atgggaatgc cattaatccc
      901 atgtctcagt ataacttctc cagtccccca ctggtcgatt atttaattaa tcggtgtttt
      961 cgcttgaacc aagatcctca tggttaaata taaccttccc aatttaaggg aaggttaatt
     1021 catttaattc aataactcta tccctcgcgg gaaaaataac aaggggcagg tgtgcccacg
     1081 caccaacaaa tggctggcga ctggacctta ttgtgtccag atttttccct caattaaatc
     1141 ttatacaagg ttatctatgc cttatctaag gttatacaaa gctaattaaa gacatttaag
     1201 gcaattaaag aaaatttaat acaattaaac cacaaatacg aggtaattca tttttctgaa
     1261 aaaaatttgt tgacaaggtt ccacaaatat cctacgatgc caaaaataac taattaaatt
     1321 gtatgacaaa gaaaaagaaa atcgtaatta cgaagataac gctggttgtt cctgcccgtc
     1381 acaactatga gacgatattc aaactggaga agtactacac caggtctgga atgataggta
     1441 taacacctac cccaatagag atcatgctta aaaacctaga caggtattcc ttcattccgt
     1501 ctgtaggttt agtgctcctc t
//
LOCUS       DAOQVW010000429         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_114186_length_1519_cov_1.956967, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000429 DAOQVW010000000
VERSION     DAOQVW010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114186_length_1519_cov_1.956967"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKK85_08280"
     CDS             complement(<1..303)
                     /locus_tag="PKK85_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS26050.1"
                     /translation="MKCIKVLLIEDNLGDAVLIQEMLKDVKTTNFQVKHAQILSEGIE
                     YLQNEDFDIILLDLALPDSQGIDTFNRANEHAAELPIIILTGLSDEEFAIKAVGEGA"
     gene            complement(300..752)
                     /locus_tag="PKK85_08285"
     CDS             complement(300..752)
                     /locus_tag="PKK85_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS26051.1"
                     /translation="MDSVNAQPLEILLVEDNPGDIRLIKEILKETKIRTNIQVAMDGE
                     AALKLLFNTENLNAAPRPDLILLDLNLPKIDGRELLSEIKCSEDLKSIPVVILTTSTA
                     EEDIIETYNNHANSYITKPVDLDHFIRVVESIKDFWLSIVKLPRSDET"
     gene            1077..1406
                     /locus_tag="PKK85_08290"
     CDS             1077..1406
                     /locus_tag="PKK85_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26052.1"
                     /translation="MQKKTEEDNFRNSMDLKDENGNVWGAVFVSTSKEFGKRDIILMD
                     ERTGTHSARSITELINMLSKKNVSFTDKKRVLEFLAERLRILEQDTKLYNFNTPVTKK
                     TSKKDKK"
BASE COUNT          488 a          244 c          263 g          524 t
ORIGIN      
        1 ggccccttct cccacagctt taatggcgaa ttcttcatct gacaggccgg tcaggattat
       61 tataggtaat tcagcggcat gttcattggc tcggttaaag gtgtcaatcc cctggctatc
      121 tggaagggct aaatccaata atattatatc gaaatcttca ttttgaagat attctattcc
      181 ttcgcttagt atttgggcat gtttaacttg aaaattagta gttttaacat ccttgagcat
      241 ttcctgtatc agaacggcat ctcctagatt gtcctctatt agaaggactt tgatgcactt
      301 catgtttcgt cactccgggg taacttaact atggaaagcc agaaatcctt gattgattca
      361 accactctaa tgaaatgatc caggtccact ggtttggtga tatatgaatt agcatgatta
      421 ttataggttt ctattatatc ttcctcggct gtagaggtgg tcagtatcac caccggtata
      481 cttttgaggt cctcactaca tttaatttcc gacaggagtt ccctaccatc tatcttgggc
      541 aggtttaaat ctaaaagtat cagatccgga cggggtgcag cgtttaagtt ttccgtattg
      601 aacaacaact ttaaagcagc ttctccatcc atggccacct gtatattagt tcgaatttta
      661 gtttccttta atatttcttt aatcagacgt atatcgcctg gattatcttc aactagaagg
      721 atttctaatg gttgtgcatt tacggaatcc atacttcctc ctcaaatatt gattttcgat
      781 agaattttgt aagtttcgaa atgattttta taagtagatt aaaaaattcg actttttact
      841 acccaaattt tcttttacca tatttatttg ccctgcttaa aattaaaatt ttcgtttatt
      901 aatttttaga aatttatata taatccatgt tctctggtaa cagtctctaa tagtttatgt
      961 ttttatatag ataaaaggtg tacgtttcac agagacttta aaatactttt ttattaaatt
     1021 ttttttcgaa acatatataa atgttgttgt agtaagatgt attaaggtga tttaagatgc
     1081 aaaaaaagac agaagaggat aatttccgca attccatgga tttaaaggac gaaaatggaa
     1141 atgtgtgggg tgctgttttt gtatctactt ccaaagaatt tggaaaaagg gacataatac
     1201 tgatggatga aagaacaggc acacattccg ctagaagtat tacagaacta attaatatgt
     1261 tatccaaaaa gaacgtgtcg tttacagata aaaaaagagt tttagaattt ttagcagaaa
     1321 gattaaggat attagaacag gataccaaac tatataattt caatactccg gttactaaaa
     1381 aaacaagtaa aaaagataaa aaatagattt attatctact agatatagat cattaacttg
     1441 accgattcat aagtttacaa attatattgg tgatatccaa tggtagatga gaaaaatttg
     1501 gaaggaactc aaggtaccg
//
LOCUS       DAOQVW010000430         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_114425_length_1517_cov_1.431601, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000430 DAOQVW010000000
VERSION     DAOQVW010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114425_length_1517_cov_1.431601"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /gene="hmdC"
                     /locus_tag="PKK85_08295"
     CDS             <1..655
                     /gene="hmdC"
                     /locus_tag="PKK85_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FeGP cofactor biosynthesis family protein"
                     /protein_id="HNS26053.1"
                     /translation="VAGATGQGKSVGLNAIITGFPKNHHGYMCGYSPGTARRGNFGLP
                     RVIKIMKEEVQSQSTGVPIGRGELEALARAVKVVGSEYLYPNKIGSFPVGDAHWVVIA
                     NSDIYRNIAVERTVKDIPEVINGESVALLGGRFISWVVAKEIDSIVDEIIISDADPWV
                     EKATVDNLRGVLNSEVKAAGSDDKKVALNSKNSIITSTVPYVVRKISRNIKGAVALI"
     gene            897..>1517
                     /locus_tag="PKK85_08300"
     CDS             897..>1517
                     /locus_tag="PKK85_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase subunit beta"
                     /protein_id="HNS26054.1"
                     /translation="MPTYEDRIDLYGADGKLLEENVPLEAISPMLNPTMEKIVHEVKR
                     SVAVNLSGIENALEKAAYGGKSNFIPGRELKLPIVENVDVLSEKIEKMIRIHDEDDFN
                     LKLINKGNQLLVQLPSQRMKMAADYTVSTLVTGSAVIQAIIDTFDVDKFNASSVKTAV
                     LGRYPQTVDFAGANITALLGPPQMLEGVGYGLRNITANHVVAITKKN"
BASE COUNT          470 a          287 c          340 g          420 t
ORIGIN      
        1 tgtggcgggt gccaccggac agggtaaatc ggtgggattg aacgccatca tcactggttt
       61 tcccaaaaat caccacggtt acatgtgtgg atattctcca ggcactgccc ggaggggaaa
      121 ttttggactc ccccgggtta tcaagatcat gaaagaagag gtacaatccc agtcaaccgg
      181 tgttccaatt gggaggggtg aactggaagc tttagcccgg gcggttaagg ttgtgggttc
      241 tgaatatcta tatccaaaca agattggttc ttttcctgtg ggtgatgctc actgggtggt
      301 tatagctaac tccgatattt atagaaatat agccgtagag agaacagtta aggatattcc
      361 tgaagtaata aatggtgaaa gtgtggcttt attagggggg aggttcatct cctgggtggt
      421 ggctaaggag atcgatagta tagtggatga gattatcatc agtgatgccg atccctgggt
      481 tgaaaaggcc acagttgata acttaagagg agtattgaat tcagaggtca aggctgcggg
      541 atcagatgat aaaaaggtgg ctttaaattc taaaaatagt ataataactt ctacagtccc
      601 ttacgttgta cgtaagattt cccgtaatat taagggggct gtggcactga tctgaaactt
      661 tattttttta ggaactattc cactttttga ggtttcactt ctttgctgct ttaacacctt
      721 tttattattt tctacctttt tattacttta aatattatat taaccctttt ttattactcc
      781 aaatttaaaa acatatcttt atatagtaag tggttttcat ttataaaata attgatattc
      841 ataatatgaa catagttcat ttttaatgaa acaaaattag aagaggtgta aaagtaatgc
      901 cgacctatga agacagaata gatctctacg gcgcagatgg aaagctttta gaggaaaatg
      961 tacctttaga agccataagt ccaatgttaa accctacgat ggagaagata gtccacgaag
     1021 tcaagcgttc cgtagcagta aacctatcag gaatagaaaa tgcactggaa aaagcagctt
     1081 acggtggtaa atccaatttt atcccaggaa gggagctgaa actacccatt gtagaaaatg
     1141 tggacgtact ttcagagaaa attgaaaaga tgatccgtat ccatgatgag gacgacttca
     1201 acctcaaact catcaacaaa gggaaccagc tcctggtaca gttaccatcc cagaggatga
     1261 aaatggctgc agactacaca gtctccaccc tggtcactgg atcagcagtt atccaggcca
     1321 tcatcgacac ctttgatgtg gataaattca acgcatcctc agtaaaaacc gcagttttag
     1381 gcagataccc ccagacagtt gattttgcag gggcaaacat caccgcctta ctgggcccac
     1441 cacagatgct ggaaggtgtg ggttacggtc ttagaaatat cacagccaac cacgtagtag
     1501 ccataaccaa gaaaaac
//
LOCUS       DAOQVW010000431         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_114851_length_1513_cov_2.908093, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000431 DAOQVW010000000
VERSION     DAOQVW010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114851_length_1513_cov_2.908093"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKK85_08305"
     CDS             <1..578
                     /locus_tag="PKK85_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HNS26055.1"
                     /translation="VNFSRKFLHIMVGNVLFILPLFDTWWVMSFLAAAPFIALTFLMS
                     PHSPVRSIRNRMSNSGHGLGLVYYSISWTVLALFFFNQPWIIAVGIAAMSYGDGMASL
                     VGMKYGKIKYNITGDVKSLEGSITMFLVLMVTLWIVLTYYGVPYTPMVMGVVALVATI
                     LEGITPKGLDNLSASFGAAATYLLMAGWIKI"
     gene            625..1206
                     /locus_tag="PKK85_08310"
     CDS             625..1206
                     /locus_tag="PKK85_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HNS26056.1"
                     /translation="MRFIVVDGLDGSGKSTHARIIREKYRKMGENVILREHPSDDNHY
                     GLKAKKALLGRGKVNKIKAAFYYTLDVLRSVRLYHQEGKTLIMVRYLMGVTYLPLPLA
                     RLLYHLFTAVLPTSDYMFFLDLEPEKALKRLNERVDEEMFENLEDLIQVRDKALQLLR
                     GWHIINTEGSIEGVQCKINSILEELDLKDKKVI"
     gene            complement(1212..1284)
                     /locus_tag="PKK85_08315"
     tRNA            complement(1212..1284)
                     /locus_tag="PKK85_08315"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1249..1251),aa:Ala,seq:cgc)
BASE COUNT          397 a          306 c          366 g          444 t
ORIGIN      
        1 atgtgaattt cagccgtaaa ttcctgcaca taatggtggg aaatgttctc ttcattttac
       61 ccctctttga cacttggtgg gtgatgagct tcctggctgc cgccccattt attgctttaa
      121 ccttcttaat gagtccccac tctccggtta ggagtattag gaataggatg tccaattcgg
      181 ggcatggtct gggcctggtt tattattcta tatcctggac tgtcctggcc ctcttcttct
      241 ttaaccagcc ctggatcata gcagtaggca tagctgccat gtcctacggg gatggtatgg
      301 cctccttagt agggatgaag tatggtaaaa ttaaatataa cataactggg gatgttaaga
      361 gcctggaagg atcgataact atgtttctgg tgttgatggt aaccctgtgg atagtgttaa
      421 cctactacgg agtaccctac actcccatgg tcatgggggt cgtggccctg gtagccacca
      481 ttctagaagg aataactccc aaagggctgg acaacctctc ggccagcttc ggtgccgcgg
      541 ccacctatct gctgatggct ggttggataa aaatttagat tacttagaac aaaactagca
      601 tgaatcttaa aatcggtgca ttgaatgcgt tttattgtgg tagatgggct ggatggttcg
      661 ggtaagagca cccacgccag gattattcgt gaaaagtatc gcaagatggg tgagaatgtt
      721 atcctccggg agcatccctc cgatgacaat cactatggac tgaaggcaaa aaaggccctt
      781 ctgggccgtg ggaaagttaa caaaataaag gcagcatttt actacaccct ggatgttctt
      841 cgctccgtga ggttatacca tcaggagggg aaaaccctca tcatggtccg gtacttgatg
      901 ggggtgacct atctacccct ccccctggcc cgattactgt accatttgtt caccgctgtg
      961 cttcccacat ctgattacat gttttttctg gatttagaac cagaaaaagc tcttaagagg
     1021 ttaaatgaac gtgttgatga ggagatgttt gagaaccttg aggatctaat ccaggttagg
     1081 gataaagcat tgcaactgct taggggttgg cacatcatca atactgaagg gagtattgag
     1141 ggcgtgcagt gcaaaattaa ttctatattg gaagaactgg acttaaagga taaaaaagtg
     1201 atctaaggaa gtggactggg cgggatttga acccgcggcc tctgccttgc gaaggcaacg
     1261 ctctaccaac tgagctacca gcccttggat tctattatta tattaaattc cttaagcttc
     1321 ttaattaact ctttgacttc tttttaaaac cttccttgaa gataattgta gttagttata
     1381 gggttatgat gattgttggg gattattaag gccgttaaaa ctggaaagag gggtattaac
     1441 tgtgtttcta ctaaaattga atagttcatc atctgcagaa agtttaggta cctattggat
     1501 ctgatatacc att
//
LOCUS       DAOQVW010000432         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_116011_length_1504_cov_2.763285, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000432 DAOQVW010000000
VERSION     DAOQVW010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116011_length_1504_cov_2.763285"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..441
                     /locus_tag="PKK85_08320"
     CDS             25..441
                     /locus_tag="PKK85_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNS26057.1"
                     /translation="MKHRHQRVLLVEDNPQHAKSIKAMLERVHEFDFVLKHCYKLQEG
                     LKIVESNEFDVILLDLGLPDSAGFETFQSIHQQTPSTPIVIFTSLFDEEIALKTLNSG
                     AQEYLFKGEYDGQDLVTAINHSIARMNFVKSLNKNY"
     gene            complement(581..>1504)
                     /locus_tag="PKK85_08325"
     CDS             complement(581..>1504)
                     /locus_tag="PKK85_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNS26058.1"
                     /translation="QLSQGANLPSEESGGKTKGMQLKSNEEMDETPEKDEEGVDIKKL
                     LKMKGKKKKRLYGRSVDSKTEKGRYIKSKLPRGDSCDVAIDATLRAAALHSEGSITVR
                     SDDIRHKVRKHGARASIAVVVDISGSMVSDRKANRVKGILDSILQDTNRHQDKISVIG
                     FKGKEAEVIVPTTKRASSFQDQVDQITVGGTTPLASGMKKGLEILKKENQKGEFVPFM
                     VILTDGLPNVGIDGGPIPDALKIAEKLQEDEILTIVINFEQTARYGRDMNMELAIASG
                     GRYYDVQDLENPGSAVSQILDFERNNLWPQA"
BASE COUNT          387 a          353 c          278 g          486 t
ORIGIN      
        1 tttcatatat atatcggtga ttttatgaaa cacaggcacc aacgtgtact tctagtggaa
       61 gacaaccctc aacatgccaa gtccattaag gccatgctgg agagggttca tgaatttgat
      121 tttgtgctta aacattgtta caaactacaa gaagggctga aaatagtgga aagtaatgaa
      181 tttgatgtga ttcttctgga tctgggcctt ccagatagtg caggttttga aacttttcaa
      241 tccatccacc aacagacccc cagtactccc attgttatat tcacatccct ctttgatgaa
      301 gaaattgcat tgaaaacttt aaattccggg gcccaggaat atctatttaa aggagaatac
      361 gatggccagg acttggttac ggctataaac cattccattg cccgtatgaa ttttgtgaag
      421 agtttgaata aaaattactg attataaagc ttaaagccta acttttttct ttgtaactga
      481 ctagtttact cttttgatac ccaactccga ccctttggaa gggttaccca atatgtggta
      541 ctcttttctg tgcctaattt tttttatacc ttccttactt ttatgcctga ggccataaat
      601 tatttctttc aaaatcaaga atctgactaa cagcagagcc agggttctca aggtcctgca
      661 catcgtagta tcggcctcct gaagcaatgg ccaattccat attcatatcc ctgccgtaac
      721 gagctgtttg ttcaaagtta atgactatgg ttaagatttc atcttcctgt agcttttccg
      781 caattttcag agcatcaggg atgggtcccc catcaattcc aacgttaggc aaaccatctg
      841 tgagtataac cataaaaggg acgaattcac ccttttgatt ctcctttttt agaatttcca
      901 aacctttttt catgccagat gccagggggg ttgttccccc cactgtgatc tggtcaactt
      961 ggtcttggaa agatgaagcc cgtttggtgg tgggtactat gacctctgct tccttccctt
     1021 tgaatccaat aacactgatt ttatcctgat gacggttggt gtcctgtaaa atactgtcca
     1081 agataccctt cacccggtta gcctttcgat cgctgaccat ggatccacta atatcgacca
     1141 ccacggcgat ggatgcccgg gcaccatgct tccgtacctt gtggcggata tcatcacttc
     1201 taaccgtaat ggagccttca gaatgaagtg cagcagcacg aagtgtagca tctatggcca
     1261 catcacaaga atctccacgg gggagtttac tcttgatata gcgtcctttt tcagttttgg
     1321 agtctacact cctgccataa agacgtttct tcttcttacc cttcattttt aacaattttt
     1381 ttatatcaac cccctcttca tctttttctg gagtttcatc catttcttca ttacttttta
     1441 gctgcattcc cttagttttc cctccagact cttccgaagg aaggttagca ccctgactca
     1501 gctg
//
LOCUS       DAOQVW010000433         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_117226_length_1495_cov_2.076389, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000433 DAOQVW010000000
VERSION     DAOQVW010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117226_length_1495_cov_2.076389"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PKK85_08330"
     CDS             <1..1415
                     /locus_tag="PKK85_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNS26059.1"
                     /translation="ARRKGTKLVVIDPVKTRTAKQADSHLKPAPGTDHYLAMALIKII
                     LKKGLEDKEFIENYTKNFTSYKGLLDKYSLEFLSKKCDVSVNELKELALSYALGKPSS
                     IVAGWGAHRYRHGHLAFHMMNALAAICGNIGVSGGGVTQGFEEYEFFDQSVELDDPGK
                     ARKLSMPKIGRAILETCDPPVELIFVASGNPVNLNPNSLIVKKAFEGKFVVMIAHFLN
                     DTSDVADLFLPATTFLEEENLIGSFGHSWISPINPVIPPQGEAKSEFEIFQLLSLRLG
                     LGDEMAGTPTKWLKKMASPILDKGVTFEELQNGPVEMVPAHEIPYADKCFKTESGLFE
                     FITDFKDDKLEGSDDGVIDENFPLHLLSTSPDKWVGSVLPESEIKNGYLEVQAHPLTL
                     KATGVADGEVALLESLFGKLEVRVRGSEDVRCDFVLAYKGGLMRHNKNINVLTGDILS
                     EKGEGAPYYDTRVRLRNLNS"
BASE COUNT          428 a          315 c          367 g          385 t
ORIGIN      
        1 aggccaggag gaagggaacc aaactggtgg taatcgatcc ggtaaagacc agaacagcca
       61 aacaggcaga cagccacctt aaaccggcac cgggcacaga ccattacctg gccatggccc
      121 ttataaaaat cattctaaag aaagggctgg aagataaaga gttcatcgag aattatacca
      181 agaatttcac atcctataaa ggattactgg ataaatattc cctggaattc ctctctaaaa
      241 agtgtgatgt gtcggtaaat gaattaaaag aattagcact ctcttatgcc ctaggtaaac
      301 cttccagtat agtggctgga tggggagctc accggtacag gcacggtcac ctggccttcc
      361 atatgatgaa cgccctggcc gcgatatgtg gaaatatcgg agtttccggg ggaggagtga
      421 cccagggatt tgaggaatac gaattcttcg accagtcggt ggaattggac gacccgggaa
      481 aggctaggaa actgtccatg cctaaaattg gccgggccat cctggaaaca tgcgatcctc
      541 ctgtggagtt gatattcgtg gcatctggaa acccagttaa cctcaacccc aactctttga
      601 ttgttaagaa ggcctttgaa gggaaattcg tagtcatgat cgctcatttc ctgaatgaca
      661 catcagatgt ggcggattta tttttaccgg ccaccacatt cttagaggaa gaaaatctta
      721 ttggaagctt tggtcatagc tggatatctc ccataaaccc cgtcatacct ccacagggtg
      781 aggcaaaatc tgaattcgag atattccagc ttctttctct aagattaggc ctgggggatg
      841 aaatggcagg tacgcccacc aagtggctta agaagatggc ttcccccata cttgataagg
      901 gtgtaacctt cgaagagctg cagaatggcc cggtggagat ggttcctgcc catgagatcc
      961 cctatgctga taaatgtttt aagacagaat caggtctctt cgaatttata acagacttta
     1021 aagatgataa gctggaagga agtgacgatg gagtaataga tgaaaatttc cccttgcatc
     1081 tgctttccac ttcccctgat aaatgggtgg gatcagtttt acctgaatca gaaataaaaa
     1141 acggctacct ggaagttcag gcacacccgt taacccttaa agcaactggt gtagcagatg
     1201 gtgaggtggc cctcctggaa tctctctttg gaaagctgga agtccgggtt cgtggaagtg
     1261 aagatgtgag atgcgacttt gtcctggctt ataagggtgg gctgatgagg cataataaaa
     1321 acatcaacgt attaactggg gacatcctca gtgagaaggg agaaggcgcc ccttattacg
     1381 atacccgggt tcgactgagg aatctgaata gttaggtttt tatctctctt tttaggtttt
     1441 atcccccttt tttttaaatg aactgttcat gactatagta aactatatat gttgg
//
LOCUS       DAOQVW010000434         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_118037_length_1489_cov_1.699442, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000434 DAOQVW010000000
VERSION     DAOQVW010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118037_length_1489_cov_1.699442"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKK85_08335"
     CDS             <1..224
                     /locus_tag="PKK85_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26060.1"
                     /translation="VIPMDEDMKYEMMIPPGVTMQMLAKLQKEYPDISIEQSDNGPLL
                     LGEKEKLEEARDYIVKALNERIKELSGGP"
     gene            complement(306..722)
                     /locus_tag="PKK85_08340"
     CDS             complement(306..722)
                     /locus_tag="PKK85_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26061.1"
                     /translation="MMDKDELIDMIVTTPIEKIEGPEDVWEAHIDLEDDKTLVVGYKG
                     EEDFCDESDEECVVTYSWYWELRNSDSWDLIQENRDGDLTFCTPSQKEPWSDESTFEE
                     IGDLSSSKICTFSDQNHIEDIAPDIADEITDWLEKD"
     gene            complement(901..1476)
                     /gene="tmk"
                     /locus_tag="PKK85_08345"
     CDS             complement(901..1476)
                     /gene="tmk"
                     /locus_tag="PKK85_08345"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNS26062.1"
                     /translation="MYICLEGIDGSGKSTQITLLEKWLNDIGQEVLRVREPTQSDVGV
                     LLRKMLASSQATEENVQRTLALLFAADRTVLMKEIEEAKSAGKVVISDRCFYSSMVYQ
                     NDVEWISQINKYAKRPDITILLDLETETALKRCEGKDHFENIDFLKDVRDKYLELAEK
                     EDFYVINAGNGVNKVHDDIKRVLSPKFGVCI"
BASE COUNT          412 a          323 c          261 g          493 t
ORIGIN      
        1 gagtgattcc catggatgaa gatatgaagt atgaaatgat gattccacca ggagtaacaa
       61 tgcagatgct ggccaagtta cagaaggaat atccagacat cagcattgaa caaagtgata
      121 atggtcccct tctactgggg gaaaaagaaa agctggaaga agccagggac tatatcgtca
      181 aggcattaaa tgaaaggatt aaggaattaa gtggtggtcc ttaactcttt tttttgaaaa
      241 taagaataag tgattgttcg tttatcatta aaggatcctt tatcattaaa agtattagat
      301 ttttatcaat ccttctccag ccagtcggtg atttcatcgg ctatatctgg agctatatcc
      361 tctatatggt tttggtcgct gaatgtacat atctttgagc tggatagatc acctatctcc
      421 tcgaaggtgc tttcatcact ccagggctcc ttctgtgaag gtgtacagaa agttaggtcg
      481 ccatcgcggt tttcctgtat aagatcccaa ctgtctgagt tacgaagttc ccagtaccag
      541 gaataggtca ccacacattc ctcatcagat tcatcacaga aatcttcctc tcccttataa
      601 ccaaccacca gtgttttatc atcttcaagg tctatatgtg cttcccagac atcctcagga
      661 ccttcaatct tctctatagg cgtggttact atcatatcta tgagttcatc cttgtccatc
      721 atctaaaaga acactcccct ttcttgatca tgtgtattac ctttaaatat tccatatctc
      781 ttgatattcc aattttttcc tatttaagcc taatcaaaga ttattatggt gaaaatagta
      841 cttaaatttt tttttatggt gattaaattt ttttgatact atttttaaaa cgaaggtaat
      901 ttagatacag actccaaatt taggtgagag aaccctcttt atatcatcat gtaccttgtt
      961 tacaccatta cccgcattta taacgtagaa atcttctttt tctgccagtt ccaggtattt
     1021 atctctgaca tcctttagaa aatcgatatt ttcgaagtga tccttgccct cacatctttt
     1081 aagtgcagtt tcagtctcca gatcaagcag tatggttata tccggccttt tcgcgtattt
     1141 atttatctgg gatatccatt ctacatcgtt ttgatacacc atactggaat agaagcatcg
     1201 atcacttatc accactttgc ctgcagattt tgcttcctct atttccttca ttaaaacggt
     1261 tcgatccgct gcaaaaagca gggccagggt tctctggacg ttttcctctg tcgcctggga
     1321 actggccagc atctttcgga gcaaaacccc cacatcagat tgtgtaggtt cccggactcg
     1381 caggacctcc tggccaatat cgttaagcca tttctcaagt aacgtaatct gggtggattt
     1441 accagaacca tcgatacctt ctaaacagat gtacatgttt ttaaccttt
//
LOCUS       DAOQVW010000435         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_118039_length_1489_cov_1.614365, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000435 DAOQVW010000000
VERSION     DAOQVW010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118039_length_1489_cov_1.614365"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKK85_08350"
     CDS             complement(<1..315)
                     /locus_tag="PKK85_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264560.1"
                     /note="the function for this protein is unknown; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HNS26063.1"
                     /translation="MAKARRRRVRDTWKEKHWYKIMTPKEFGEAEIGTTPARDPSMLI
                     RRRVESSLRELSGDFSKQYIKLYFQINDVAGDTASTNFVGHQVTSDYVRSMIRRGTSR
                     IDA"
     gene            complement(781..1113)
                     /locus_tag="PKK85_08355"
     CDS             complement(781..1113)
                     /locus_tag="PKK85_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26064.1"
                     /translation="MLICVFAVLIWILPIYALDLGNTTSNLSVNSTGAQNQIDSYSVG
                     SQITMLKSTSAGKYKDIQDLHLKILHILEGMRLLRQQNNTHPVRYYVELETISAELER
                     LKRSMIGC"
BASE COUNT          434 a          271 c          263 g          521 t
ORIGIN      
        1 tgcatcaatt cgactggtcc ctctccttat catgcttcta acataatctg aggttacctg
       61 atgccctacg aagttggtgc tagccgtgtc tccggccaca tcgttgatct ggaagtaaag
      121 tttaatgtac tgtttgctga aatctccgga gagctcccgg agggatgatt ccacccttct
      181 tcttatgagc atgctgggat cccgggcagg agtggtgcct atttctgctt ctccaaactc
      241 cttaggggtc ataatcttat accagtgttt ttctttccat gtatcgcgta ctcgtctacg
      301 tcttgctttc gccataatat caccttgaaa cgtgtttaaa agaatatttg ttaaatataa
      361 attctttaag ttccataaaa cggaagatta ttttattaat aattctccat ttctagttta
      421 taaatattgt ctgatctgat ttacggataa tttgttgata tccagttaac tcagtacaat
      481 cttattcaat taactgagat agtaaattgt ttactcaatt aactctgctg taaattgttc
      541 attatatctt gtggcatgat ccttacgttg ataagtggaa caatttaaaa aaagtttttc
      601 atttaaaaag ttggttttac cgccaggtat gttgatagga catagttaga atttcatggt
      661 atataggtgt ttaggttttt aaagtggtga ttgatttaac atcaaaagag tatttgaata
      721 acatatcttg aataacatat ctatttaaaa aaagtttaaa gaggggggtt taaaaccctc
      781 ttaacatcca atcatgctgc gtttaagtct ttctagttct gcagatatgg tttccaattc
      841 aacatagtat ctgactggat gcgtattatt ttgctggcgt aataatcgca ttccttccag
      901 tatatgtagt attttcaggt gtaaatcctg aatatccttg tatttccctg cagatgtact
      961 tttaagcatg gttatctgac tcccaactga atagctatct atctggtttt gagccccggt
     1021 tgaattaacg cttagattag atgtggtatt acctaaatcc agggcgtaaa taggcagaat
     1081 ccagatgaga accgcaaaca cacagattaa cactaatttt ctccagccat tatcacctcc
     1141 ctattaatgg atatgataga ttgatactca gcttaaaaat agtttttcca gattcaggcc
     1201 caaatctcac cagtttaccc ggaacagagg atacaaaaat agaatttata ataaataatc
     1261 attatggaat gtatttgaat ggctaattcc acagatgaat ctcacggaag cgtattaagc
     1321 atgtttaata tttgggcttt taaaatttga gaggtcgatt catgaattga taatgtttaa
     1381 cgtaacgccc taaaacttca tcaaaggttc cttcatcctc tactacagtt ttacccatct
     1441 tcctgagtgc tggtttagcg tttataccga tttgtatgca gatgattac
//
LOCUS       DAOQVW010000436         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_118273_length_1487_cov_2.224162, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000436 DAOQVW010000000
VERSION     DAOQVW010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118273_length_1487_cov_2.224162"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PKK85_08360"
     CDS             <1..502
                     /locus_tag="PKK85_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HNS26065.1"
                     /translation="QITRAVKDLRVYVDPREPVFIIVISYEKTAPPVRLLDFAEYEKN
                     REANEVFLKIKDETYLPELLAALWETEGRNKVHQSSRYEVIVDDPRTDIKNMVVHNPE
                     EDLRKKIYDALFRIIPEGLRVVDHYSEGNYIALTASDETIKDEWLQKTQEIIRDMKAS
                     ENRYKV"
     gene            664..>1487
                     /locus_tag="PKK85_08365"
     CDS             664..>1487
                     /locus_tag="PKK85_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS26066.1"
                     /translation="MNESKFAHLTKVHPCFNEKMHDKVGRIHVPIAPKCNIQCNFCTR
                     EINKCEMRPGVASCIMSVEDAIAHVDKVTKEMPISVVGVAGPGDALFNPETFQFFREM
                     DVRHPELIKCISTNGLLLPDKADELAEININSITVTVNAIDPEIGKEIYSLAIHHGKV
                     YDKEEAFDVISKNQLEGIKLIAENDIVVKVNSVLIPGLNDEHIVDIARKVKAQGASLM
                     NVIPLIPLYKFKDTPKPDCMALSTVRDAVEEIIPVFRACTQCRADAYGVPGKEDKHL"
BASE COUNT          501 a          309 c          349 g          328 t
ORIGIN      
        1 acagattacc agggcagtga aggatttaag ggtctacgta gatcccagag aaccggtgtt
       61 catcatagtg ataagctacg agaaaacagc ccctcctgtg aggttactgg acttcgcgga
      121 gtatgaaaaa aacagggaag caaatgaggt ttttttgaag atcaaggatg aaacctacct
      181 tccagaacta ttagccgcat tatgggagac tgaaggaagg aataaggtgc atcaatccag
      241 cagatacgag gttatagtgg atgatccccg taccgatatt aaaaatatgg tggtgcacaa
      301 ccccgaagag gacctgcgga agaaaatata cgatgccctg tttagaataa ttcctgaggg
      361 tttgagggtg gtggatcact actcagaggg taattatata gctcttacag cttctgatga
      421 gactataaag gatgaatggc tccagaagac ccaggaaatc ataagggata tgaaggcttc
      481 ggaaaataga tacaaagtct aaaacgagaa aagtattatt actcccgaac taatatatta
      541 tgttactgtt agaacaaatc aggcttactc ttaaaaacaa aatcaagtac atcattaaca
      601 atcaagtaac attcatctac atttattaac gtatgaataa gctgaggatg gaggaaaaag
      661 ataatgaacg aatccaaatt cgcccattta accaaggtac acccttgctt taacgagaaa
      721 atgcatgata aagtgggacg gatacatgtc cccatcgctc ctaagtgtaa catccagtgt
      781 aacttctgca cccgggagat aaacaaatgt gagatgaggc cgggggtagc gtcctgtata
      841 atgagcgtgg aagatgccat cgcacacgtg gataaggtga ccaaggaaat gcccatatcc
      901 gtggtgggag tggccggtcc aggtgacgcc ctgttcaacc cggagacctt ccagttcttc
      961 cgggagatgg atgtaagaca tcccgaactc ataaaatgta taagtaccaa cggactgctt
     1021 ttacccgata aagcggatga actggctgag atcaacataa acagtatcac cgtaactgta
     1081 aacgccatag acccggagat aggtaaggaa atctattcct tagccataca ccacggcaaa
     1141 gtctacgata aagaggaagc ctttgatgtg atatccaaga atcaactaga gggtataaag
     1201 ctcatcgcag aaaatgacat cgtggtcaag gtaaacagcg tcctgatacc cggattaaac
     1261 gatgaacaca tagtggatat cgcccggaaa gtcaaagccc agggcgcatc actgatgaat
     1321 gtaatcccac tcatcccact ctacaaattt aaagacacac caaaaccaga ctgtatggca
     1381 ctaagtacgg tgagagacgc cgttgaagag ataatacccg tattccgggc ctgcacccag
     1441 tgccgtgccg atgcctatgg tgtgcctgga aaagaagata agcacct
//
LOCUS       DAOQVW010000437         1476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_119666_length_1476_cov_19.173821, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000437 DAOQVW010000000
VERSION     DAOQVW010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119666_length_1476_cov_19.173821"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..179
                     /locus_tag="PKK85_08370"
     CDS             42..179
                     /locus_tag="PKK85_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26067.1"
                     /translation="MAEVGYIRPENFQDIQKYHDRLIKRWRHNGIVHKRPLYGDWVVL
                     K"
     gene            176..322
                     /locus_tag="PKK85_08375"
     CDS             176..322
                     /locus_tag="PKK85_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26068.1"
                     /translation="MSNCQAKDRETGEIIDLGTCQDKIECLIRLLEYANRLDIKGGNQ
                     DGSS"
     gene            309..494
                     /locus_tag="PKK85_08380"
     CDS             309..494
                     /locus_tag="PKK85_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26069.1"
                     /translation="MAVHKYRRLDPPRASRGPGVFPALWVRFPSGLLIPLFFLGGVIA
                     GMVLIGLGMDPRPEWWV"
     gene            498..638
                     /locus_tag="PKK85_08385"
     CDS             498..638
                     /locus_tag="PKK85_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26070.1"
                     /translation="MSSTTIFLDNYMPGGAGDFMLAAVIIMTMLLLLVIYLAVTRDHD
                     WR"
     gene            644..838
                     /locus_tag="PKK85_08390"
     CDS             644..838
                     /locus_tag="PKK85_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26071.1"
                     /translation="MESSKRIHHPRAYYQEHTRDPVIEAAARELFNLLNKNGFYSIED
                     LGAIANIILKSEGKTPRRQW"
     gene            840..1100
                     /locus_tag="PKK85_08395"
     CDS             840..1100
                     /locus_tag="PKK85_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26072.1"
                     /translation="MERRIIEFRRAKEDEECSTLEVMAVALSDEDHKNTREELKGLGF
                     RFDVIESDMIKATLSDCDPDLALTADELLILAEKLESTGWVW"
     gene            1104..1385
                     /locus_tag="PKK85_08400"
     CDS             1104..1385
                     /locus_tag="PKK85_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26073.1"
                     /translation="MVKDSFKFDNEKNQLLIDGEHMELVITFKSDRYREIVSKTRRLA
                     MEYDIKELFRRVSYENPEFRDGLNNMFGVLLMADLPSSEWKKQTDYLLK"
BASE COUNT          425 a          279 c          368 g          404 t
ORIGIN      
        1 gccggttgtt aggggtaaga tggcttttat agggtgatcg tatggctgaa gttggatata
       61 tacggcctga aaacttccag gacatccaaa agtatcatga ccgattgatt aaacggtgga
      121 gacataatgg gatagtccat aagaggcccc tttatggtga ttgggtggtg ctaaaatgag
      181 taactgccaa gcaaaagacc gggaaacggg cgaaataata gaccttggaa catgccagga
      241 taaaatagaa tgcttgatcc gcctcctgga atatgcaaac cggttagaca ttaaaggagg
      301 taatcaagat ggcagttcat aaataccggc ggcttgaccc tcccagggcc tcccggggcc
      361 ccggagtgtt cccggccctc tgggttcggt tcccctctgg cctgttgata cctctttttt
      421 tccttggtgg tgtcattgct ggcatggtat taatcggatt gggcatggac ccccggcctg
      481 agtggtgggt ttaaaatatg tcctccacca ctattttttt agataattac atgccaggtg
      541 gagccggtga ttttatgctg gcagcggtta ttattatgac catgctccta ttattggtca
      601 tatacttagc agtgacccgg gatcatgatt ggaggtaata tttatggaga gctcaaagag
      661 aatacaccac ccccgggcct attaccagga acacacccgg gacccggtga tcgaagcggc
      721 tgccagggaa ttattcaatt tacttaataa gaatggcttc tattccattg aggacctggg
      781 agcaatagcc aatattatac ttaagagtga gggaaaaaca ccaaggagac aatggtaaaa
      841 tggaaagaag aatcatagaa tttagaagag ctaaagaaga tgaagaatgc agcaccttag
      901 aggtgatggc agtagcctta tctgatgaag accataaaaa cacccgagag gaattaaaag
      961 gcctgggttt ccgtttcgat gtgatcgaaa gtgatatgat caaggccacc ctctccgatt
     1021 gtgatccgga tcttgcctta actgctgatg aactcctaat tcttgctgaa aagttagaat
     1081 ccaccgggtg ggtgtggtag atcatggtta aggattcttt caaattcgac aatgaaaaaa
     1141 accagctttt gatcgatggt gagcacatgg aattagtgat caccttcaaa tctgaccggt
     1201 accgtgagat tgtgagtaag acccgccgcc tggcaatgga atatgatatt aaggaattgt
     1261 ttaggagggt gagttatgag aaccccgagt ttcgggatgg tttgaataac atgttcgggg
     1321 tcttgcttat ggctgatctg ccatcgagtg aatggaagaa acaaacggat tatttgctta
     1381 aataaaaatt tctttttttt tacttttttt aactatcatt caaccgttac actgataatt
     1441 aggagataat atttattaca ttaagttcac ttgacg
//
LOCUS       DAOQVW010000438         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_120294_length_1472_cov_1.664785, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000438 DAOQVW010000000
VERSION     DAOQVW010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120294_length_1472_cov_1.664785"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PKK85_08405"
     CDS             complement(<1..762)
                     /locus_tag="PKK85_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNS26074.1"
                     /translation="MDDDDLRQLVFKYALVNATGHSGQARNGAVMGMIMAQHPELRSQ
                     AKRIGPLTSQVVAQVNQMSQMSQEEKLEELGGHQAPEKKGEGERELPPLPGAEDGVVL
                     RFAPNPSGPLHIGHARAAILNQAYQERYGGKLILRVEDTDPRRVYPPAYKMIQEDLAW
                     LGVEWQEEVVQSQRLEIYYEVAEEAILAGAAYMCTCPGDEFKKLKDSGVACPHRAMSP
                     QENLNLWEKMPEYRKRGAVLRIKTDLEHKNPAIRDW"
     gene            complement(846..>1472)
                     /locus_tag="PKK85_08410"
     CDS             complement(846..>1472)
                     /locus_tag="PKK85_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNS26075.1"
                     /translation="VLWTETDGMDSRLVVGALQTVADSCVNICEGQALDMGFADRLAV
                     SEEEYLHMIYKKTAALIAAATRSGAILGGGSEEQVEALAEYGRLIGLAFQIQDDYLDV
                     TSDEDNLGKPVGSDIAEGKRTLLVVHTLEKAKPQDRDELIRILREKSDQSVARAIEIF
                     QEYGSIDYAHDIALGNVEKAKKLLLILEDTPAREALMKLADFVLERSH"
BASE COUNT          233 a          475 c          397 g          367 t
ORIGIN      
        1 ccagtccctg atggccgggt ttttatgttc cagatccgtt ttgatcctta aaactgcccc
       61 tcttttcctg tattcaggca tcttctccca taagtttagg ttctcctggg ggctcatcgc
      121 ccggtggggg caggccactc cagagtcctt gagtttcttg aactcatccc cggggcaggt
      181 gcacatgtag gccgccccag ccaggatggc ctcctctgcc acttcgtagt agatttccag
      241 gcgctggctc tgcaccacct cctcctgcca ctcaactccc agccaggcca ggtcctcctg
      301 gatcatcttg taggctgggg ggtacacccg gcgggggtcg gtgtcctcca cccggagtat
      361 gagcttgcca ccgtagcgtt cctggtaggc ctggttcagg atggcggccc gtgcatggcc
      421 tatgtggagg ggtccgcttg ggttaggggc gaatctcagc accaccccat cctcggcacc
      481 gggtagtggg ggcagttccc tctccccctc cccttttttc tccggggcct ggtgtccgcc
      541 cagttcttcc agcttctctt cctgggacat ctgggacatc tggttgacct gggccaccac
      601 ctggctggtg aggggtccta tccttttggc ctggctgcgc agttcagggt gctgggccat
      661 gatcatgccc atgaccgccc cattccgggc ctgtccagag tggccagtgg catttaccag
      721 ggcgtactta aagaccagtt gacgtagatc atcatcatcc atcgctatcc acctaagaat
      781 tagttaatca gaataaatag gggggactct ttcatgttaa tcccggcccc ccctacatgt
      841 gaacattagt ggcttctctc cagtacgaag tctgctagct tcatcagggc ctcccgggcc
      901 ggggtgtcct ccaggatcag gagtagtttc ttggcctttt ccacgttgcc cagggcgatg
      961 tcatgggcgt agtctatgct cccatactcc tggaagatct ctatagcccg ggctacgctc
     1021 tggtcgcttt tctccctcag gatccggatg agctcgtccc ggtcctgggg tttggccttc
     1081 tccagggtat gcaccaccag tagggttctt ttcccctctg cgatgtcact gcccactggc
     1141 ttccccaggt tgtcctcgtc gcttgtcaca tccaagtagt cgtcctgtat ctggaaggcc
     1201 aggccaataa gcctcccata ctcggccagg gcctctacct gttcttcgct tccaccaccc
     1261 agtatggcgc cggagcgggt ggcggcggcg atgagggcgg cggttttctt gtagatcatg
     1321 tggaggtact cctcctctga cacggccagg cggtcggcga agcccatgtc cagggcctgc
     1381 ccctcacaga tattcacaca actatcagcc acggtctgca gggcccccac caccaggcga
     1441 ctgtccatcc cgtctgtttc cgtccacagc ac
//
LOCUS       DAOQVW010000439         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_121124_length_1465_cov_3.846809, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000439 DAOQVW010000000
VERSION     DAOQVW010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121124_length_1465_cov_3.846809"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..653
                     /locus_tag="PKK85_08415"
     CDS             45..653
                     /locus_tag="PKK85_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HNS26076.1"
                     /translation="MEPGFILSFLAGIASLLSPCVLPLVPIVIGYFILNRNLREVLAF
                     TGGFFLIFAVITVLTMIFTAALNVYLLYIRIVAAILLVLVGIFFIINPKIPSLSIKSR
                     GEGGATGSFIMGLLTCLAWSPCFGPYVIAVAAYSTATGNLLYSALNMILFAAGFSLTL
                     FALAILASRINWERTTKYTKTIRIISGLVIALAGIYMLGGLL"
     gene            676..>1465
                     /locus_tag="PKK85_08420"
     CDS             676..>1465
                     /locus_tag="PKK85_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HNS26077.1"
                     /translation="MKVGFIGFGEVASTLARGLMGVDVEIYTCLEGRSVRTRELARLM
                     NVQLCSSHREMAQNVDMILSAVTPSQAVMVARKLGRIRGVYVDLNNVSPSTVRTALEL
                     VGKANTVDAAIIGSVLKKGLKVNIVASGPAAAEFAQLNDYGMDIDVIGPDPGQASAIK
                     MMRSAYTKGVSALLLESLFAAHRMGIDDEALKYISQTECPDFQEKAVSRMTSSLYHAR
                     RRSEEMEEVVKMLSSETEPFMSRASLEFFQLLTERIGKMDEKPSN"
BASE COUNT          384 a          326 c          381 g          374 t
ORIGIN      
        1 ccaatcagct gaccagttat taagccaact ataggggtat gtggatggaa ccaggtttta
       61 tattatcgtt cctggcggga atagcttccc tactatcgcc ctgcgtgctg ccacttgtac
      121 ccatcgtgat tggatacttc atcttaaacc ggaatttaag ggaagttcta gcattcaccg
      181 gtggattttt tctaatattc gcagtcatca ccgtactgac gatgatattc actgcagccc
      241 tgaatgtata cttgctctac atacggatag tggcggccat tctcctggta ttggtgggga
      301 tttttttcat catcaacccc aaaattccca gcttatcaat caaatccagg ggggagggcg
      361 gagctacagg gtcctttata atgggactcc taacctgcct ggcctggtct ccctgtttcg
      421 gtccctatgt gattgcagtg gcagcttaca gcacagccac tggcaacctg ctctacagtg
      481 ccctgaacat gatcctcttt gcggcaggtt tctccctcac cctctttgca ctggccatac
      541 tggcgtccag aattaactgg gagagaacta caaaatatac aaagacgata aggatcatat
      601 ccggcctggt catagccctg gcaggaatct acatgttagg gggattgtta tgaatcagga
      661 aatgggggtg cacagatgaa ggtgggtttt ataggttttg gagaagtagc ctcaaccctg
      721 gcccggggtc ttatgggggt ggacgtggag atctacacct gtctggaggg aagaagtgtc
      781 cgtaccaggg aattagcccg gttaatgaat gttcaactgt gcagcagcca ccgggaaatg
      841 gcacagaatg tggatatgat cttatcggca gtaacaccat cccaggcagt gatggtggcc
      901 agaaaattgg gccggatcag gggggtttat gtggacctca acaacgtgtc accctcaacc
      961 gtcaggacag ctctagaact ggtgggaaaa gccaacaccg tggacgcagc tatcattggc
     1021 agtgtcctga agaagggttt gaaggtgaat atcgtagcat ctggtccggc tgcagcagaa
     1081 tttgcccagt taaacgatta tggtatggat attgatgtta tagggccaga tccaggtcaa
     1141 gcttctgcca ttaagatgat gcgcagtgcc tacacaaagg gagtgtcggc acttctcctg
     1201 gaatcattat ttgccgccca ccgtatggga atcgatgatg aagcgttaaa atatatatcc
     1261 cagacagaat gtcccgattt tcaagaaaaa gcagtttcta ggatgaccag cagcctttat
     1321 catgcgcgaa gaagatctga ggagatggaa gaagtggtta agatgttatc ttcagaaact
     1381 gaacctttta tgagcagggc ttccctggaa tttttccagc ttctaaccga aaggataggg
     1441 aaaatggatg agaagccaag caaca
//
LOCUS       DAOQVW010000440         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_121486_length_1463_cov_2.325994, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000440 DAOQVW010000000
VERSION     DAOQVW010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121486_length_1463_cov_2.325994"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKK85_08425"
     CDS             <1..659
                     /locus_tag="PKK85_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNS26078.1"
                     /translation="MLTREVPSKYVKYIPLLVSGDFYDFLNEERYSPLRDASEFSTAV
                     NACSRHDQPEIALKVLKGNRGIALQDMQSLTKKHRRYLAQKMEWIENEGQIKSLENIR
                     YFEGNEIKSNVIGTIAGMLLTYGDDQEKMEGEKKQWQKPMMAFTEMGDEKDGLKVSLR
                     CSRLLAYDGIHFGNLIKKVADKVGGTGGGHSVACGAYIPTEKKEDFLKIFNNSITGLI
                     "
     gene            735..>1463
                     /locus_tag="PKK85_08430"
     CDS             735..>1463
                     /locus_tag="PKK85_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNS26079.1"
                     /translation="MKVFKKKGEITRFQILAEIARGDPHLRQKDIANKMGITIQAVSE
                     NIKNLTDEGLVETGRGNFRYKITKRGIDKVKKEALDLRKYADQVLDTMNTYKSVWPAL
                     ALEDLAAGERVWLRMVDGTLFAGKDKSPAHAEVLHDAHKGEDVALSSLGGTIELEPGS
                     VVIVKLPPINQGGSKKTDLKKVRRIMVGSEKDFDRVGIMGTVSRAVVDKIGIKPDFEF
                     ATPQATVAASKRGLNILVFAVGKMT"
BASE COUNT          485 a          266 c          361 g          351 t
ORIGIN      
        1 agatgttgac acgagaagta ccctcaaaat atgttaaata catccctctc ctggtttctg
       61 gtgacttcta cgatttttta aacgaggaaa ggtactcccc actccgtgat gccagtgaat
      121 tttcaacagc agttaacgcc tgcagccgcc atgaccagcc cgaaatagct ttaaaagtcc
      181 ttaaaggtaa tcgtggaata gcattacaag acatgcagag cttgaccaaa aaacatcgga
      241 gatacctggc tcaaaagatg gaatggatag aaaatgaagg tcaaattaag tccctggaaa
      301 atattcgcta ttttgaggga aatgaaatta aaagcaacgt aattggaacc atagctggca
      361 tgttgttaac ctacggtgat gatcaggaaa agatggaggg agaaaaaaaa caatggcaaa
      421 agcccatgat ggcttttaca gaaatgggag acgaaaaaga tggtctaaaa gtttccctac
      481 gctgttcaag gcttttggct tacgacggaa tacattttgg caacttgata aaaaaagtgg
      541 ctgacaaggt tggagggact ggagggggac attccgtggc ttgtggcgct tatattccca
      601 ctgaaaagaa agaagacttc ctaaaaatat ttaacaactc cattactggt ttaatttagt
      661 tgagtttagt agctccatca aataaaagta aagttccccc tataaagtgg ttaaaaaaac
      721 acttggtgat ggtaatgaaa gttttcaaaa aaaaaggtga aataacccgt ttccagatac
      781 tggctgagat agccagggga gatccgcatc tacgccagaa ggatatagcc aataaaatgg
      841 gtataaccat ccaggcggtt tctgagaata ttaaaaacct gacagatgag ggtctagtgg
      901 aaactggccg cggaaacttc cgctacaaaa tcacaaaacg aggtatagac aaggttaaaa
      961 aagaggcact tgatcttcgt aaatatgcag atcaggttct ggataccatg aacacataca
     1021 aatcagtgtg gccggccctg gctctggaag acttggctgc tggagaaagg gtgtggttga
     1081 ggatggtgga tggaacgcta ttcgcaggaa aggataaatc accagcccat gccgaggttc
     1141 ttcatgatgc acataaaggt gaagatgttg ccttaagtag tctgggtgga accatagaat
     1201 tggagccagg atccgtggta atagtaaagc ttccccccat taatcagggc gggtctaaga
     1261 agacagatct aaaaaaggtg cgcaggatta tggtaggatc tgaaaaggat tttgaccggg
     1321 taggcattat gggaaccgtg tcccgggccg tggtggataa aattggaatc aagcctgatt
     1381 tcgaatttgc aactcctcaa gcaacggtag cagcttctaa aaggggactt aatatcctag
     1441 tatttgctgt gggtaaaatg acc
//
LOCUS       DAOQVW010000441         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_121488_length_1463_cov_2.273438, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000441 DAOQVW010000000
VERSION     DAOQVW010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121488_length_1463_cov_2.273438"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..348
                     /locus_tag="PKK85_08435"
     CDS             97..348
                     /locus_tag="PKK85_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2109 domain-containing protein"
                     /protein_id="HNS26080.1"
                     /translation="MITEIVGIIVLFAAVRTLIAQDRSERMLYLNVIGFGMSALIALY
                     IQTPFGAIIAITYFVASTLSSNAIAYSIGRVKDEIILDD"
     gene            432..722
                     /locus_tag="PKK85_08440"
     CDS             432..722
                     /locus_tag="PKK85_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2108 domain-containing protein"
                     /protein_id="HNS26081.1"
                     /translation="MALLEFINITYVSAAVVIIGSVGIIALPKPIDKVIMFGLLQGGF
                     IGMIAAARYLDVAMAAAIFDPISTVILLIGITKLDEIRKKKQKQAQEEGFLV"
     gene            715..975
                     /locus_tag="PKK85_08445"
     CDS             715..975
                     /locus_tag="PKK85_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EhaE family protein"
                     /protein_id="HNS26082.1"
                     /translation="MFDIYVCFYTGVLLVILGSIATAIGPGVKDPIVRTLNTEIPSVG
                     ISLILLTYNHTIALLTFIAATVIIMMILLRAIVRMEELGAEL"
     gene            972..1451
                     /locus_tag="PKK85_08450"
     CDS             972..1451
                     /locus_tag="PKK85_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EhaF family protein"
                     /protein_id="HNS26083.1"
                     /translation="MKLGRIWNSLATPKNIPKVFSIILGLVLLAGFIVPLTYNDHQLY
                     PRPTPQLQISEKDPLAPYNRGGIPLEEPGIVKAQYPQFAPSEGMVTGYLSPIAIGVSN
                     TTLYFGTSIYSSPGGLIDEILYYSRGFDTILESSILMMAFVIASWVALHFTMRRREE"
BASE COUNT          466 a          291 c          299 g          407 t
ORIGIN      
        1 aagaatactg aagatccaag aaataataaa gaatatatat tcataattgt taaaatagat
       61 taaggcaatt tgaagaatag ttctaagggt aaatgtatga taacagaaat agtaggcatc
      121 attgttttgt ttgcagcagt aaggaccctt attgcccagg acagatcaga acgtatgctg
      181 tatttaaatg taataggttt tggtatgtcc gcactcattg ccctttatat acagacgcca
      241 tttggggcta taatagccat aacttacttc gtggcatcta ccttaagttc caacgccata
      301 gcctactcca ttggaagagt taaagatgaa attattttag atgattaact agaatattct
      361 aaatgccaat taagaggggt ttggtttttt aaagtgaagt taactgtatc aagaatgaag
      421 gtgtaaaacc aatggcacta ttagagttca taaatataac ctatgtttcg gctgccgtcg
      481 ttatcatagg ttcagtaggt ataatagcac ttcctaaacc aattgacaag gttataatgt
      541 ttggattgct ccagggcggt ttcataggta tgatcgcggc tgcaagatac ctggatgtag
      601 ccatggccgc tgctatattc gaccctatct ccacagtaat ccttctaatt gggataacta
      661 aactggatga gatacggaag aaaaaacaga aacaagcaca ggaggaagga ttccttgttt
      721 gatatttacg tatgctttta taccggtgta ttactggtta tattaggcag catagccaca
      781 gccataggtc ctggagtaaa agaccccata gtaaggaccc tcaacactga aataccatct
      841 gtaggaattt cattaatctt actgacctat aaccacacca tcgcactttt aaccttcata
      901 gctgcaaccg ttataatcat gatgatactc ttgagagcca tagtgagaat ggaagaactg
      961 ggggctgaac tatgaaattg ggacgaatct ggaattcatt ggccaccccc aagaatatac
     1021 ccaaagtttt ctctataatt ttgggactgg tacttttagc tggctttatt gtaccattaa
     1081 cctataacga tcaccaatta taccccagac caaccccaca attacagatc agtgaaaaag
     1141 acccccttgc accatacaat cggggaggga taccactgga agagccgggt atagttaaag
     1201 cccagtatcc acagtttgca ccatcagaag gtatggttac gggctattta tccccaatag
     1261 ccattggcgt aagtaacaca accctatact tcggtacatc catctattcc tctccaggag
     1321 gattaataga tgagatactt tactacagca ggggtttcga cacaatccta gagtccagca
     1381 tcctgatgat ggcattcgtc atagcctcat gggtggcgct gcacttcacc atgagaagga
     1441 gggaggaata aaaatggcag cgt
//
LOCUS       DAOQVW010000442         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_122007_length_1459_cov_3.000000, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000442 DAOQVW010000000
VERSION     DAOQVW010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122007_length_1459_cov_3.000000"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1459)
                     /locus_tag="PKK85_08455"
     CDS             complement(<1..>1459)
                     /locus_tag="PKK85_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HNS26084.1"
                     /translation="HFCQFYQRREDLVEVLVPYFRAGLEDNEFCLWIASEPLGAEEAR
                     QVLVDAVPGLECYLERGQMEILSCQEWHHGGLNPGEVMEDWIGRLDQARSRGFDGLRL
                     GGKIFWVEKGVWADLIQCEEVLNQVLGGYPMMALCTYPLDKCSSRDVLDIISTHKFTV
                     ARRDGLWELMEGANHTQMDLQSKVEAAFSSINEAVITTDTAGNIIDMNEAFAHFHRFP
                     SLDECSHHLDDYPHLFNVYWPDGTPAPQDRWAVPRALAGEQASNIEYLIERTDTGEVW
                     NSSYSFAPIRNKEGEITGTVVLMRDTTQARKRELELKRVNERLNIASRAAGAGIWDWD
                     IETGMIEWSPLMFELLGLDPQTPPSFQAWESVIHPEDHGKAREQVTMAVQNHTFMDNL
                     YRIIKPGGEVRWINDLGQTEYDQDGRPLWMTGICLDTTERREMELKYQELVQNARSII
                     IRWDLEGRLNFFNEYAEEVFGYSSQEVLGQLVMDVL"
BASE COUNT          299 a          453 c          374 g          333 t
ORIGIN      
        1 agcacatcca tcaccaactg gcccagaact tcctgggaac tgtagccaaa gacctcctca
       61 gcgtactcgt tgaagaaatt caaacgcccc tccaggtccc agcggatgat gatgctccgg
      121 gcattctgca ccagttcctg gtacttcagc tccatttccc tccgttcggt ggtgtccagg
      181 cagatgccag tcatccacaa gggacggcca tcctggtcat actcggtttg tcccaggtcg
      241 ttgatccacc tcacctcccc gccgggtttt atgatccggt acaggttgtc catgaaggta
      301 tgattctgca ctgccatggt gacttgttcc ctggctttcc catggtcttc agggtgtatc
      361 actgactccc aggcctggaa ggagggaggg gtctgggggt ccagtccaag gagttcgaac
      421 atcaggggcg accattcaat catcccggtt tcaatatccc agtcccagat accagccccc
      481 gcggctcggg aggcaatgtt caggcgttcg ttaacccgtt tcagttccag ttctcgtttt
      541 ctggcctggg tggtgtcccg catgagcacc acggtaccgg tgatctctcc ctccttgttt
      601 cttatggggg cgaagctgta actactgttc cacacctctc cagtgtcggt tctttcaatg
      661 aggtactcga tgttggaggc ctgctcaccc gccaatgccc ggggcaccgc ccacctatcc
      721 tggggggcgg gggtgccatc gggccagtag acattgaaaa gatggggata atcatccaga
      781 tgatgggagc attcatccag actagggaag cggtggaagt gggcgaaggc ctcgttcatg
      841 tcgatgatgt tcccggcagt gtcggtggtg atcaccgcct cgttgatact ggaaaaggca
      901 gcttctacct tggattgcag atccatctgg gtatggttcg ccccctccat cagctcccac
      961 aatccatccc tccgggccac cgtgaatttg tgggtgctga tgatgtccag gacatccctg
     1021 gaggagcatt tatccagggg ataagtgcaa agggccatca tggggtatcc tcccaggacc
     1081 tggttcagca cctcctcgca ctggatgaga tcagcccaca cacccttctc cacccagaag
     1141 atcttgccgc ccagcctcaa accatcaaaa cccctggacc gggcctggtc tagcctgcca
     1201 atccagtcct ccatgacctc cccggggttt aaaccaccat ggtgccactc ctggcagctg
     1261 aggatctcca tttgacccct ctccaggtaa cactcaagac cggggacagc atccaccagg
     1321 acctgcctgg cttcttcagc ccccaatggc tccgaggcga tccacaagca gaattcattg
     1381 tcctctaaac cagccctgaa gtagggaacc agtacctcca ccaagtcttc cctcctctgg
     1441 taaaactggc agaaatggg
//
LOCUS       DAOQVW010000443         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_122395_length_1456_cov_4.122769, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000443 DAOQVW010000000
VERSION     DAOQVW010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122395_length_1456_cov_4.122769"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKK85_08460"
     CDS             complement(<1..669)
                     /locus_tag="PKK85_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNS26085.1"
                     /translation="MIILDENTKCVVQGITGKQGSFHTQSMLEYGTNIVAGTSPGKGG
                     QNFQGVPVYNSIEETLEETEVNASVIFIPAPFAKDAAFEALSQLDTVVVITEHIPIHD
                     TLEIVAYARENNKILIGPNTPGIITPGIGKLGIMPAHIFNPGDVGIVSRSGTLTYEVA
                     SQITQAGMGQSTCLGIGGDPVVGLDYSDILQRFQDDPETRAMVMIGEIGGNAEEKAAE
                     FIAAN"
     gene            complement(732..>1456)
                     /locus_tag="PKK85_08465"
     CDS             complement(732..>1456)
                     /locus_tag="PKK85_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26086.1"
                     /translation="LTSIGADISRNDVHCSLELVEKIERDIPQLILAGAQKAALTNVT
                     GDDLVISAFLEDENLEKVNQAIVEILKKNAEDWGDIHGISPTPEGAGEGISYAEAMVR
                     DGRFPDAVILSFDTYGGESFVHHVAESAMNAAAGMEGVTDIGGSSADGVRNIPGVGYV
                     SDMTDDPVVAVTVEDLESIGAIAGAMVGAALGNKNVYMVKRGSPSYVIPGSVILSVSA
                     YMNGNVMDLAGPLSQRMRILKV"
BASE COUNT          323 a          408 c          333 g          392 t
ORIGIN      
        1 gttggcggct atgaactccg ctgccttctc ttcagcatta cccccgatct ctccgatcat
       61 gaccatggcc cgggtttcag ggtcatcctg gaacctctgg aggatgtcgc tgtaatccag
      121 tcctaccact gggtcgccgc caatacccag acaggtgctc tggcccatcc cggcctgggt
      181 gatctgactg gccacctcgt aggtgagggt gccactgcga gacactatac ccacatcacc
      241 tggattgaag atgtgagcag gcatgatgcc cagtttacca ataccagggg tgataatccc
      301 gggagtgtta gggcctatta ggattttatt gttttcacgg gcgtaggcca caatttccag
      361 ggtgtcgtgg atggggatgt gttcggtgat caccaccaca gtgtccaact gtgagagggc
      421 ttcaaaggcc gcgtccttgg cgaatggcgc gggaatgaat attacggagg cgttaacctc
      481 tgtttcctcc agggtttcct ctatggagtt gtatacaggt acgccctgga agttctggcc
      541 acctttaccc ggggaagttc cagccacgat gttggtaccg tattccagca tgctctgggt
      601 gtggaaggaa ccctgttttc cagttatacc ctgcaccaca cacttcgtat tttcatcgag
      661 aattatcact ctataacacc ttccatcaat aaacgtagtt tttgttaatc tttcttacca
      721 gcccactcca ttcacacctt caatattctc atacgctggg ataagggtcc ggccagatcc
      781 atgacgttac cgttcatgta ggcgctgact gacagaatca cacttcctgg gatgacatag
      841 gagggtgaac ctcttttcac catgtacaca ttcttatttc ccagagccgc acctaccatg
      901 gctccggcta ttgctccaat actctccaag tcttccacgg tcacagccac cactggatcg
      961 tcggtcatat cagaaacgta acccactcca ggtatatttc tgactccatc cgcactgcta
     1021 cctccaatgt cggtcacacc ttccatgcct gccgcggcat tcatggcgga ctcggccaca
     1081 tggtgcacga aggattcacc accataggta tcaaaggata agataacagc atctggaaat
     1141 cggccatctc ttaccatggc ttcagcgtat gatatgccct ctcctgcccc ttctggtgtg
     1201 ggggatatac catggatatc tccccagtct tcggcatttt tcttcaaaat ctccactatg
     1261 gcctggttta ctttttccag attttcatcc tctagaaagg cactgatgac caagtcatcc
     1321 ccggttacat tggtcaaggc ggctttttgg gcgccagcca agatcaattg gggtatatcc
     1381 ctttcaattt tttcaaccag ttccagactg cagtgcacat cgtttcggga aatatcggca
     1441 ccaatggaag ttaatt
//
LOCUS       DAOQVW010000444         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_123407_length_1449_cov_2.967001, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000444 DAOQVW010000000
VERSION     DAOQVW010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123407_length_1449_cov_2.967001"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKK85_08470"
     CDS             <1..310
                     /locus_tag="PKK85_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNS26087.1"
                     /translation="LGMLLESSGRGGTMELGRIPRNKMVTWEDWLKLYPGAGFVLTVA
                     EENVPECERILKEVNITCGVVGTVIPENKLFLTHDGEKEIVFNFQSESIMGVREKINR
                     "
     gene            316..>1449
                     /locus_tag="PKK85_08475"
     CDS             316..>1449
                     /locus_tag="PKK85_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HNS26088.1"
                     /translation="MKVQVNGDEIELTSGSTIRDAIEATQAPYLPGSVLGVVKGREEV
                     ERHVNKYRLKTPQGSIILELLKEAPPELVDLWKKRYHDFSGLRVRWTTSKEASVGPVI
                     TDLEPSHDEYTYQRWDVIFSLSGFTADATHIIFSKDKHEAVYGAPKGNDGVFARVVGG
                     KRTIMKLSDDDHVLEVTPVVERRSIVKSAAIIDLETELSEGNQVFTYLQVEPEPKSPQ
                     SVEHFYALSESGKLGVDYDSNSFLGSYSLQGIKRDIEWIDKRKRGTVTLRNQGKGMGR
                     VYIYREDRVSTPSHNMVGKVVKGMELVDIARYSDEVTLRIKPERIMTLAMTQIEAEEF
                     LSDHGVKQVRSGLEDDEALVVRQEPNYTMQIIEKGEATTYGVRN"
BASE COUNT          444 a          293 c          406 g          306 t
ORIGIN      
        1 gttaggaatg ttactggaat cctcagggag gggtggaacc atggaactgg gcagaatacc
       61 ccgcaacaaa atggtgacct gggaggactg gctaaaactc tatcccgggg caggttttgt
      121 cctaactgta gccgaagaaa atgttcccga atgcgaaagg atattgaaag aggtcaatat
      181 aacatgtggc gtggtgggaa cagttatacc ggagaataag ctgtttttaa cccatgatgg
      241 agagaaggag atcgttttta acttccagag tgaaagtata atgggagtga gagagaagat
      301 aaacaggtga gatccatgaa ggtgcaagtt aatggagatg aaatagaact taccagtggt
      361 tccaccataa gggatgccat tgaagcgacc caggccccat atctacctgg aagtgttctg
      421 ggagtggtta agggccggga agaagtggaa agacacgtga acaagtaccg tttgaaaact
      481 ccgcagggga gtataattct ggagctacta aaggaagctc caccggaact tgttgatctg
      541 tggaagaaac gttaccatga cttttcaggg ctaagagtgc gctggacaac ctccaaggaa
      601 gcctcggtgg ggcctgtgat cactgatcta gaaccatccc atgatgaata cacctaccag
      661 cgctgggatg tgatattcag tttatccgga ttcaccgccg acgccaccca catcatattc
      721 agcaaagaca aacacgaagc tgtttacgga gcccctaagg ggaatgacgg agtttttgcc
      781 cgggtggtgg gtggtaaaag gaccataatg aaactaagcg atgatgacca tgtcctggag
      841 gtcacacctg tagtggaaag aaggagcatt gtgaaaagtg ccgccatcat agacttggaa
      901 acagagctat ccgaggggaa ccaggtgttc acatacttac aagtagaacc cgaaccaaaa
      961 tcaccccaat cagtggaaca cttctacgcc ctctccgaat cgggaaaatt aggggtggac
     1021 tatgattcca actcattcct aggatcttac agtcttcaag gaattaaaag agatatagaa
     1081 tggatagaca agcgtaaaag aggcacagtg accctgcgta atcagggtaa gggtatgggt
     1141 cgggtttaca tctaccggga ggaccgggtg tccactccct cccacaacat ggtgggtaag
     1201 gtggttaagg ggatggaact ggtggacata gcccgttaca gtgatgaagt aacactccgt
     1261 ataaaacctg agaggataat gaccctggcc atgactcaga ttgaagcaga ggaattttta
     1321 tcagaccatg gtgtgaagca agtgcggagc ggcttggaag atgatgaggc cttggtggtc
     1381 cgccaggagc caaattacac catgcagatc atagaaaagg gagaagccac cacctatgga
     1441 gttaggaac
//
LOCUS       DAOQVW010000445         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_123528_length_1448_cov_4.236899, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000445 DAOQVW010000000
VERSION     DAOQVW010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123528_length_1448_cov_4.236899"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKK85_08480"
     CDS             <1..326
                     /locus_tag="PKK85_08480"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HNS26089.1"
                     /translation="APHWHRDPKEYMEEIIELGFQVIITAVAADGFEKTWLGREINQE
                     VLDELQVLHEKHGIHLAFEGGEAETLTLDGPIFKKKVIIQESEKNWQVDSGQLTVKKA
                     ILGDK"
     gene            complement(342..740)
                     /locus_tag="PKK85_08485"
     CDS             complement(342..740)
                     /locus_tag="PKK85_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HNS26090.1"
                     /translation="MNCKIRVTSRVNPTEDPQKVVEALSNVFDYDDLRLGEGYVVVSG
                     EVSCLQPFKEHLEKGRIRDTARKILGQGRSPGEITFKLSKQAAYVGKVNLVEDELSSL
                     GELEVTIKADNTDEFIDWLCEKKEDDEEVY"
     gene            complement(715..1260)
                     /locus_tag="PKK85_08490"
     CDS             complement(715..1260)
                     /locus_tag="PKK85_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNS26091.1"
                     /translation="MMVLGVTGMPGAGKGVVARVATDLKIQVVRMGDVIREEASNRNE
                     PPGVVAVKLRQEYGEFVVAQRCVDIIQDSLEKDTSYLIEGIRSPFEVEIFQKNFPGFK
                     VIAVHSKPKTRFKRLKRRKRSDDSSSEDEFFSRDKRELKFGIGDVIAMADYMVVNEGS
                     IIKIKKIVRSILKNELQNQGY"
     gene            complement(1257..>1448)
                     /locus_tag="PKK85_08495"
     CDS             complement(1257..>1448)
                     /locus_tag="PKK85_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate/pantothenate synthetase
                     family protein"
                     /protein_id="HNS26092.1"
                     /translation="RSSSITIVDNLVRALPLLIQEVEKLKGEEMYKLEEMLSDFNNPE
                     NLNDSLQAILRNYHRGVKP"
BASE COUNT          395 a          372 c          272 g          409 t
ORIGIN      
        1 tagcacccca ctggcaccgg gatcccaagg agtacatgga agagataata gagttgggat
       61 tccaggtaat tataactgca gtggctgcag atggttttga aaaaacctgg ctgggccgtg
      121 aaatcaacca ggaggtactg gatgaactcc aagtcctgca tgaaaagcac ggcattcacc
      181 tggccttcga gggtggagaa gcagaaaccc tcaccctgga tggtccaata ttcaaaaaaa
      241 aggtaattat tcaggaatct gaaaagaact ggcaggtgga tagtggacag ttaacagtta
      301 aaaaggctat attaggggat aaataatcca cagaattcta gctaataaac ttcttcatca
      361 tcttcttttt tctcacagag ccagtctata aattcatcag tgttatcggc tttaatggtg
      421 acctctagct cccccagtga ggaaagttca tcctccacca gattcacttt acccacatag
      481 gcagcctgct tacttaattt aaaggttatc tccccgggag atctcccttg gcctaatatt
      541 ttcctggcag tatctctgat gcggcctttt tccaagtgtt ccttaaaggg ctggaggcag
      601 gaaacttctc cagataccac cacgtaaccc tcacccaacc ttaaatcatc gtaatcaaaa
      661 acatttgaca atgcttcaac aaccttctga gggtcttccg taggattcac ccgactagta
      721 accctgattt tgcaattcat tcttcagtat actcctcact atcttcttaa ttttaataat
      781 agaaccttca ttaaccacca tataatcggc catggcaata acatcaccaa taccaaattt
      841 cagctcccgt ttgtcccttg aaaagaattc atcctcacta gaggagtcat ctgatctttt
      901 tcttcgtttt agccgtttaa acctggtttt tggcttagaa tgtactgcta tgaccttgaa
      961 gccaggaaaa tttttctgaa atatttccac ttcaaagggg ctgcgaatac cttcaatgag
     1021 gtaggatgta tccttttcca gggagtcttg aatgatgtcc acgcagcgct gggccactac
     1081 aaattctcca tactcctggc gcagcttcac cgccaccacc cctggaggtt cattcctgtt
     1141 actggcctct tcacgtatca catcccccat acgcactacc tggatcttca gatcagttgc
     1201 caccctagcc accactcctt tacctgcacc tggcatccca gttactccca gaaccatcaa
     1261 ggtttcacac ccctgtggta atttcttagg atggcttgaa gggaatcatt gagattctct
     1321 gggttgttaa aatcactcag catctcttct agtttgtaca tttcctctcc tttaagcttt
     1381 tccacttcct gaatcaaaag aggcaaagcc cgcaccagat tgtccacgat ggttatggac
     1441 gaactacg
//
LOCUS       DAOQVW010000446         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_123671_length_1447_cov_3.714080, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000446 DAOQVW010000000
VERSION     DAOQVW010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123671_length_1447_cov_3.714080"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="PKK85_08500"
     CDS             <1..116
                     /locus_tag="PKK85_08500"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HNS26093.1"
                     /translation="KLDQFDDELVKKGLNPGTTADLTASSIMAAYLADYKE"
     gene            151..>1447
                     /locus_tag="PKK85_08505"
     CDS             151..>1447
                     /locus_tag="PKK85_08505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNS26094.1"
                     /translation="MLDRIKEEMHLYEKKVLKALESEGGESTPERVLKLQLLPIKSVM
                     SAAGILASKNIIKVNKDVDELLSLSEDGASYAQDGLPERRILKALGKEETVDMKDLSQ
                     KSGIEPSEVKIAIGWLLRKGWATLDKGQVTISKHGKRALEEAGPDENLLSKLEESGEL
                     SLQELSPRLQEGFNLLKQRKGLLKVHKKPSYTLEVTTLGKEILEDGVEIREEATQITH
                     EQLKSGSWRNLHYRAYDVKAEHPESFPGKLHPLQRTIEEIRRIFLNLGFTESRGTILE
                     SAFWNFDCLFQPQDHAAREMQDTFYIKTPRETLLPDEELVERVKKAHEHGGKTGSKGW
                     NYQWDREVARQSVLRTHTTCVSARFLKDNQPPLKMFSVGRVFRRETITYKHLPEFHQV
                     EGIVADKSINFRNLLGILKEFYHQLGFQVRFRPAYFPYTY"
BASE COUNT          415 a          341 c          372 g          319 t
ORIGIN      
        1 ataaactaga ccagttcgat gatgaactgg tgaaaaaagg tttgaatcct ggtactacag
       61 ccgatctgac tgcttcttct attatggcag cttatctggc cgattataaa gaataaactt
      121 tatatgatga ttgattaatg aggtatttta atgctggata gaattaaaga ggagatgcac
      181 ctttacgaaa agaaggtctt gaaggccctg gagtcagaag gaggagagag cactccagag
      241 agggtgttga aacttcaact tttaccaatc aaatccgtga tgagtgccgc tggtattcta
      301 gcctctaaaa atattattaa ggtaaacaaa gatgtggatg aactcctgag cctctctgag
      361 gatggcgcat cctatgcaca ggatggactg cctgaacgga ggatactaaa ggccctggga
      421 aaagaagaaa ctgtagatat gaaggattta tcccagaagt ctggtattga accatccgag
      481 gtgaaaatcg ccattggctg gttactccgg aaaggctggg ccaccctgga taagggccag
      541 gtcaccatca gtaagcatgg gaaaagggca ctggaagagg ctggtcctga tgaaaattta
      601 ctctccaaac tggaagaaag tggtgagtta tccttacaag aactttcccc tcgacttcaa
      661 gaaggtttta atcttttaaa acaaaggaag gggctcctta aggttcataa gaaacccagc
      721 tacacactag aagtcaccac cctgggcaag gagatattgg aggatggagt tgagatcagg
      781 gaggaagcca ctcagatcac ccacgaacag cttaaaagtg gtagctggag gaatctgcac
      841 taccgggcct acgatgtaaa ggctgagcac ccggaaagct tccctggtaa gctccacccc
      901 ctgcagagga ccattgagga gataagacgt atcttcctaa atctgggctt caccgagtcc
      961 aggggaacta tacttgaatc tgctttctgg aacttcgact gcctcttcca gccccaggac
     1021 cacgctgccc gggagatgca ggacaccttc tacattaaaa ctccccggga gaccctgcta
     1081 cccgacgagg aactggtgga gagggttaaa aaggcccatg aacacggtgg aaaaacgggt
     1141 tctaagggct ggaactacca atgggaccgg gaagtagccc ggcagtcggt gctgcgcacc
     1201 cacaccacct gtgtctcagc ccggttttta aaggacaacc agccccccct gaagatgttc
     1261 tcggtgggcc gggtcttccg ccgggagacc atcacctaca agcacctgcc agagttccac
     1321 caggtggagg gtatcgtggc tgacaagagc atcaacttcc gaaacctcct ggggatctta
     1381 aaggagtttt atcatcaact gggcttccag gtacgattcc gaccggctta cttcccctac
     1441 acctatc
//
LOCUS       DAOQVW010000447         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_123683_length_1447_cov_3.305316, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000447 DAOQVW010000000
VERSION     DAOQVW010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123683_length_1447_cov_3.305316"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /gene="lysS"
                     /locus_tag="PKK85_08510"
     CDS             complement(<1..493)
                     /gene="lysS"
                     /locus_tag="PKK85_08510"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNS26095.1"
                     /translation="MKHWTERIASDLTNWNVEEHVVASGTSISGSIHIGNSCDVFIAN
                     AVGKSLQKLGEKSRTIWIADDHDPLRKVPYPLPESYEKYLGIPYSQIPCPKDCCESFV
                     EHFQKPFLDTLPVFGIELDTYSGAKMYQEGVYNDYIKKSLERAPKIREIFNHHREKPL
                     KDDW"
     gene            complement(554..>1447)
                     /gene="thiC"
                     /locus_tag="PKK85_08515"
     CDS             complement(554..>1447)
                     /gene="thiC"
                     /locus_tag="PKK85_08515"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase"
                     /protein_id="HNS26096.1"
                     /translation="TIYMDEHTMFKTIEKQAKDGIDFMAIHCSVNMETLKRLKRQGRK
                     GGLVSRGGALISAWMVENELENPLYKNYDYILEIAKEYDFVMSMANAMRAGAIADATD
                     RAGVQELIILGELIDRAREVGVQTIVEGPGHIPLKEIEANVVIQKKLCREAPFYMLGP
                     IVTDIAPAYDHIVSSIGAAQSSASGADFICYVTPAEHLALPGPEDVKMGVIASRIGAY
                     VGDMSKGIHNGELDLEMANARKKLNWEAQYQASICPNDARAIRDNRPPEDPDTCTMCG
                     SYCAVKIVNEWLDEAPTATFE"
BASE COUNT          342 a          339 c          316 g          450 t
ORIGIN      
        1 accaatcatc tttcagaggt ttttcccggt gatggttaaa tatttccctg attttaggtg
       61 ctctttccag tgatttttta atataatcgt tatacactcc ctcctggtac atctttgccc
      121 cggaataggt atctaattct ataccaaaca ccggcagggt gtccaggaag ggtttctgga
      181 agtgttccac gaaactctca cagcaatctt tggggcatgg tatctgggag taagggatgc
      241 ccaggtattt ttcataggat tcgggcaggg gatagggtac cttacgcagt ggatcgtggt
      301 catcggctat ccagatggtc ctggattttt caccaagctt ttgcagtgat ttaccaactg
      361 cgtttgcaat aaaaacgtca catgaatttc caatatgaat agatcctgag atagaagttc
      421 cactggctac tacgtgttct tcaacgttcc aattagtaag atcagatgca attctttcgg
      481 tccagtgttt caattagttc accttctgct aaaataaaaa attggggaaa aaaatttttt
      541 acgtgtattt tttttattcg aatgttgcag ttggagcttc atccaaccat tcattcacga
      601 ttttaacggc acagtagcta ccacacatgg tacaggtatc cgggtcttct ggtggccgat
      661 tatcccttat ggctcgtgca tcattaggac atatggaggc ttgatattgt gcttcccagt
      721 tgagcttctt ccgggcgttg gccatttcca gatctagttc tccattgtgt atacctttgg
      781 acatgtctcc tacgtaggca cctatgcggc tggcgataac tcccatttta acgtcttctg
      841 gtccgggtaa ggccaagtgt tcggcagggg tcacgtaaca tatgaagtct gctccagagg
      901 ctgaagattg ggcagcacct atggaagaaa cgatatggtc ataggcaggt gcaatatccg
      961 ttacaattgg ccctaacatg tagaaaggag cttctcggca cagttttttc tgaattacca
     1021 cgttggcctc gatttccttc agtggtatat gccctggtcc ctctacaatg gtctgcaccc
     1081 ccacttcacg ggcccggtcg attaactctc ccagtataat aagttcctgt actcccgcac
     1141 gatcagtggc atcagcaatg gctccagccc tcatggcgtt agccatagac atcacaaagt
     1201 cgtattcctt agcaatttcc agaatgtagt cgtaattctt atataagggg ttttccaatt
     1261 cattttcgac catccatgca gatattaatg ccccgcctcg gctaaccagt ccgccctttc
     1321 ggccctgtct tttcagacgc ttcagggtct ccatgttcac actgcagtga atggccatga
     1381 agtcaatacc atcttttgcc tgtttttcga tggttttaaa catggtgtgt tcgtccatgt
     1441 aaatggt
//
LOCUS       DAOQVW010000448         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_123703_length_1447_cov_2.801724, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000448 DAOQVW010000000
VERSION     DAOQVW010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123703_length_1447_cov_2.801724"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKK85_08520"
     CDS             <1..718
                     /locus_tag="PKK85_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HNS26097.1"
                     /translation="RVLVSLLVSRETENLSKNELASATVESLTENINDSVTAPIFYVL
                     LIGTLVMLLCAYFNINPQYSPHNVKWLWEIKNPLTISKYSFTLLTLPILAGVFYRVVN
                     TLDAMVGYKTSEYRMIGYFPAKLDDVLNFLPARFTGVLVVIAAAINGLDWRNSWKTMR
                     KEASKTSSPNSGFTMAAAAGALNVQLEKPSAYKLGKFGYDLDGYIIKKAILLTKITII
                     LFLILSFTMYFILYLIVTRV"
     gene            834..>1447
                     /locus_tag="PKK85_08525"
     CDS             834..>1447
                     /locus_tag="PKK85_08525"
                     /inference="COORDINATES: protein motif:HMM:NF023188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiG"
                     /protein_id="HNS26098.1"
                     /translation="MRFAIISITKKGVVLADNLLQALSEDPTVIQVDAYVGDVKNTFM
                     KIYPDYDCIIAIMATGIVVRTICSLLKNKVTDPAILVVDENGENVVSLLSGHLGGANE
                     YTLKVSQVLNANPVITTSTDVSKKMGVDTLARRHWLSLDNLNIVKALNRAIADGEILD
                     LYLPTRYDFVEDDKIARDTYKFHYWDDNNIRVDFEEISLNLNPLT"
BASE COUNT          455 a          268 c          280 g          444 t
ORIGIN      
        1 acgtgtatta gtatcattat tagttagtag ggaaaccgaa aatttatcaa agaatgaatt
       61 agcctcagct actgtggaaa gcctcacaga aaatattaac gattcagtca ccgcacccat
      121 attctacgtt ttattaattg gaaccctggt catgctccta tgtgcttact tcaacattaa
      181 tccccaatac tccccccata atgtcaagtg gctctgggaa attaaaaatc ctttaaccat
      241 ttcaaagtat tcattcacgc tactaacact ccccatccta gctggagttt tctatcgcgt
      301 ggttaacacc ctagacgcta tggttgggta taaaacatct gaatacagaa tgattggata
      361 cttccctgct aagttagacg atgtccttaa ttttttacct gcaaggttca ctggcgttct
      421 ggtggtaata gccgctgcaa ttaatggtct tgattggaga aactcctgga agaccatgag
      481 gaaagaggcc agtaaaacgt ctagtcctaa ttctggtttc acaatggcag ctgcagccgg
      541 tgcattaaat gtacaattgg aaaagccaag tgcctataaa ttgggaaagt ttggctatga
      601 tttggatggt tacattataa aaaaagcaat tttacttact aaaatcacca tcattttgtt
      661 cttaatccta agcttcacca tgtactttat cttatatctc atagttacaa gggtataaag
      721 ggattgggat aataaacatc taaattcaaa tagataacac aactgcttgg aattagtgtt
      781 atcgatattg attaaaaaaa catgaataaa tcaagtctat agggaggtca tctatgagat
      841 ttgccatcat cagcattacc aaaaagggtg ttgtcctggc cgataattta cttcaagctt
      901 tatccgagga tcccaccgtc atacaagttg atgcttatgt gggtgatgtg aaaaacactt
      961 ttatgaagat ctatcctgat tatgattgta taattgccat tatggccact ggaatagtgg
     1021 taaggactat ctgcagttta ttaaaaaata aagtaactga tccagcaatt ctggtcgttg
     1081 atgagaatgg tgaaaatgtt gtaagtcttt tatcaggcca tctggggggg gctaatgaat
     1141 ataccttgaa ggtttcccaa gttttaaacg ctaatccagt gataaccact tccacggatg
     1201 tttcaaagaa gatgggtgtg gatacactgg cccgcaggca ctggctttca cttgataatc
     1261 ttaatatagt taaagccctt aatagagcca tcgctgatgg ggaaatcctg gatctatatc
     1321 tacccactcg ttacgatttc gtagaggatg ataaaattgc cagggatact tataaattcc
     1381 attactggga tgataacaat atacgagttg attttgaaga gattagcctt aatttaaatc
     1441 cattaac
//
LOCUS       DAOQVW010000449         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_124088_length_1444_cov_3.509719, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000449 DAOQVW010000000
VERSION     DAOQVW010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124088_length_1444_cov_3.509719"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKK85_08530"
     CDS             complement(<1..386)
                     /locus_tag="PKK85_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS26099.1"
                     /translation="MNKSLEIDPQIEGILEKAFHDPISTEEALKLMETTGDNFHALVI
                     AADKFRKDLIGDKVTYIQNWNINFTNICTGTCGFCAFRKKKGEKEAYFLAIEEIVQRA
                     RRAWKNGAVEVCIQGGLHPAVDAYFY"
     assembly_gap    444..453
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            544..750
                     /locus_tag="PKK85_08535"
     CDS             544..750
                     /locus_tag="PKK85_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HNS26100.1"
                     /translation="MAELPIAPVGRIIKNAGAQRISDDAKEALAKALEEKGEELAKQA
                     VKLAKHAGRKTVKADDIDMAVKSA"
     gene            924..1415
                     /locus_tag="PKK85_08540"
     CDS             924..1415
                     /locus_tag="PKK85_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNS26101.1"
                     /translation="MSTMDNLKEAFAGESQANHKYMAFARKADEEGYTQIAKLFRAAA
                     EAERVHAHNHFTIMGGISSTEENLKMAISGEIDEFENMYPDFIEEAKKDGNKKAVWTF
                     DVANQVEQIHAVLYKNALESIGNTEEVDYYVCGCCGNTVENEAPDICPVCKCPKVEFF
                     RVE"
BASE COUNT          449 a          269 c          291 g          425 t
ORIGIN      
        1 tcataaaaat aggcatctac agcaggatgc agaccaccct ggatgcagac ctccacggcc
       61 ccatttttcc aagctctcct ggccctctgg actatttctt caatggcaag gaagtatgct
      121 tctttttcac ccttcttttt gcgaaaggca cagaagccac aggtcccggt gcagatgttg
      181 gtgaagttta tattccaatt ctgtatgtag gtgactttat ctccaatcag atcttttcga
      241 aatttgtctg cagctataac tagggcatga aaattatctc cagttgtttc cattaattta
      301 agggcttctt cagtggaaat agggtcatga aatgcttttt ctagtatacc ttctatctgg
      361 gggtcaatct ccagactctt attcatgaca attcctatag cgtacaataa ccttataact
      421 tttggttaga tgatgaactc atcnnnnnnn nnngtggata gtgggtatac acttgcccag
      481 aagaccatta ataccgatat atttaaatat aatagtgccc tagctaaatt aggaggtgaa
      541 aatatggctg aattaccaat tgctccagtt ggaaggatca taaaaaatgc tggtgcacaa
      601 cggattagtg acgacgcaaa agaggcttta gctaaagctc tcgaagaaaa aggagaggaa
      661 ctggccaaac aagctgttaa acttgcaaaa cacgccggaa ggaaaactgt caaagccgac
      721 gatattgaca tggccgttaa gtccgcttaa ttgtcttctc ttttgtattt ttggggatta
      781 atccctactt tatatttttt ttctcctttt ttttgcaaaa ccaattaaat actaaattgc
      841 atattagcta ctttcatctt caaccaaaaa tttaatagac ttcatactta aataaatctg
      901 ttaaattatg gaaaggaatg tatatgagca ccatggataa tttaaaagaa gcatttgctg
      961 gtgaatctca ggcaaaccat aaatatatgg cgtttgctag aaaagccgat gaagaaggtt
     1021 atactcagat agcaaagtta ttccgggctg ctgctgaggc tgaaagagtg catgctcaca
     1081 accacttcac cattatgggt ggtatcagct ccaccgaaga gaacctaaaa atggcgatat
     1141 ctggagagat tgatgaattt gagaacatgt atcccgattt cattgaagaa gctaaaaaag
     1201 atggcaacaa aaaggcagta tggacctttg acgttgcaaa ccaggtggaa cagatacatg
     1261 cagtattgta caagaacgcc ctggaatcca ttggtaacac agaagaagtg gattactatg
     1321 tatgtggctg ttgtggcaac acggtagaaa atgaggcccc tgacatatgt ccagtgtgta
     1381 aatgtcctaa agttgagttt ttcagggtag aataagtaaa atatcttctt attctctttt
     1441 tttt
//
LOCUS       DAOQVW010000450         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_124424_length_1442_cov_2.381399, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000450 DAOQVW010000000
VERSION     DAOQVW010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124424_length_1442_cov_2.381399"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..>1442)
                     /gene="hemA"
                     /locus_tag="PKK85_08545"
     CDS             complement(284..>1442)
                     /gene="hemA"
                     /locus_tag="PKK85_08545"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNS26102.1"
                     /translation="DIAQIERATRKLEDIFERCQSDDDIQEHLYIKTCNRSEIYLVAG
                     KLQEDLLSGGDFIVDQDEEALRHLLRLASGLESMILGEDQILGQIKDARKKSIRECSI
                     GPVLRTVFTKAIHVGQLARNNTRINQGTISIGSAAVELAESVHGDLKCKKVLIIGAGK
                     MGTLVAKALVEKKLKAIVVANRTYGSAVKLAGELKGYAIHFDKLHEAMADADVVISAT
                     GAPHQILTYDQVKESVLPENRGSLVMVDLANPRDIDEKVKDLGVKLFNIDDLRDIADN
                     SVKMRQLEVSNVEKIVEDELKLLLKSLKHLEVEPLISNIRICAEEVRLAETRKAFKML
                     GDLNGKEKIVDDLTQVVIDRIFFDVIKNLRKAAEQDDDDTIRAAEKIFSGE"
BASE COUNT          360 a          340 c          274 g          468 t
ORIGIN      
        1 tttcctgttt ttaatcatat caccatcaac tgggttttga ttttaatttt tggataattt
       61 cccggggatt aaatagtaag atttatatgt aacctctcac taacattatg tttgaggggg
      121 ggtgctaaaa taaagagaaa gagaaaagga atagatttca accaatcccg gaatatccac
      181 caaaaattaa tcaaacaaca gttcctttta tccatgctta gaaaattaga ttatgatctg
      241 aacacatgaa aaatctggta actggaattt agttatcagt tattcattca ccagagaata
      301 ttttctccgc cgcccgaata gtatcatcat catcctgttc tgcggctttc cttaaatttt
      361 taatcacatc gaagaatatc cggtctatta ccacctgggt taaatcatcc acaatctttt
      421 ctttaccatt caggtcgccc aacatcttaa aagctttcct ggtttcagcc agcctgactt
      481 cctcggcaca gatccggata ttggatatga gtggttccac ttccaggtgt ttaagtgatt
      541 ttaatagtag ttttagttcg tcctctacta ttttttccac atttgaaact tctagttgcc
      601 tcattttcac gctgttatcg gctatgtccc ttaaatcgtc tatattaaat agtttaaccc
      661 ctaaatcttt caccttttca tctatgtccc ggggattggc caggtccacc atgaccaggc
      721 tgccccgatt ttcaggtaga acagattctt taacctgatc gtaagttaat atttggtgtg
      781 gagcacccgt ggcacttata accacatcag catctgccat ggcctcatgg agtttgtcga
      841 agtgtatggc gtatccttta agttcaccgg ccagtttaac cgcgctcccg taggttcggt
      901 tggccactac tatggctttc agtttctttt caaccaaggc tttggccacc agtgtcccca
      961 ttttacccgc ccctatgatc agcacctttt tgcacttcaa atcaccgtgc acagattctg
     1021 ccagttccac agccgcagaa ccaatggata tggtgccctg atttatgcgg gtgttatttc
     1081 tggctaattg tcccacgtgt atggctttgg taaaaacagt tctaaggaca ggacctatgg
     1141 aacattcccg gatagatttc ttccgggcgt cctttatctg gcccagtatc tggtcttccc
     1201 ctaaaatcat agattcaagg ccggatgcca ggcgtaaaag atgtctgagg gcctcttcat
     1261 cctggtctac tatgaagtca cctcccgaga gaaggtcttc ttgaagtttg cccgctacca
     1321 ggtagatttc agaccggtta caggtcttta tgtagaggtg ttcctgaata tcatcatcac
     1381 tctggcatct ttcgaagata tcctcaagtt ttctggttgc ccgttctatc tgggcgatat
     1441 ct
//
LOCUS       DAOQVW010000451         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_124727_length_1440_cov_2.178339, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000451 DAOQVW010000000
VERSION     DAOQVW010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124727_length_1440_cov_2.178339"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1440)
                     /gene="topA"
                     /locus_tag="PKK85_08550"
     CDS             complement(<1..>1440)
                     /gene="topA"
                     /locus_tag="PKK85_08550"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HNS26103.1"
                     /translation="VQTPALAILVDREKEIGTFIPEPYWNIKAYLEGDIIADHKKGKI
                     FKREDVDEILADCKDKDAQVSKIELKTSKKIPPFPFDLGSLQSEAYSLFGFSPKRTQQ
                     IAQNLYSEGYTSYPRTSSQKLPKSIGYDKILKKLSGNAAFRKTIHKLKKPYKPREGKK
                     KDEAHPSIHPTGVLPKKLGRDYQKLYELIAYRFISVFGEDALVETMKTNLEIGKQPFT
                     FTRKRTAKLGWQEQYPFRSVENDEFPNIQEGDKLKGEVKDEEKETKPPARYNQASLIR
                     ELEKRGLGTKSTRANIISILYDRKYVEGKKIEVNVLGEQLIDTLVKYSQKITSEELTR
                     EFETKLENIQKGKTQKENIIEEYKTEISSILDDIEENKLKIGQELYKAYRESMIVGEC
                     KCGKNLILISSRQGNPFVGCSGYPECKNTYSLPRGATILKTKCEECGLPMISFGKPRQ
                     RACLDPKCGKEDQEPSNEVVGECPECGSEL"
BASE COUNT          295 a          327 c          312 g          506 t
ORIGIN      
        1 agttcactgc cacattccgg gcattcaccc accacttcat tggacggttc ctgatcctct
       61 ttaccgcact taggatccag gcaggccctc tgtctgggtt ttccaaagga tatcatggga
      121 agtccgcatt cctcacactt cgtcttgagg atggttgccc ctcggggtag tgaataggta
      181 tttttacatt ctggatatcc agaacaacca acgaatgggt ttccctgacg agaactgatg
      241 aggatcaggt ttttaccgca tttacactca ccgacgatca tactctcccg gtaagcttta
      301 tacaattctt gaccgatttt gagtttgttt tcctcaatat catccagaat agagcttatt
      361 tctgttttat attcctctat aatattttct ttctgggttt tacccttctg tatattttca
      421 agtttggttt caaactccct ggttaattct tcactggtta tcttctggga atacttcacc
      481 agggtgtcta taagctgttc gcccagtacg tttacctcta tcttctttcc ttccacatac
      541 tttctatcgt aaaggatcga tatgatatta gcccgggtgg atttagttcc cagtcccctt
      601 ttttctaatt cccgtattaa agaagcctga ttataacgtg ctgggggttt ggtttctttt
      661 tcttcatctt taacctcacc cttcagctta tcgccttcct ggatgttagg gaattcatcg
      721 ttttcaacgc tcctgaaggg gtactgctcc tgccatccca gtttagccgt tctcttcctg
      781 gtgaaggtga agggttgctt tcctatctcc aggtttgtct tcatggtctc taccagtgca
      841 tcttcaccga aaacacttat aaaccggtag gctatgagtt cgtaaagttt ctgataatct
      901 ctacccagtt ttttaggcag aactccggtg gggtgtatgg atgggtgtgc ttcatccttt
      961 ttcttacctt cgcgtggctt gtatggcttc ttgagtttat ggattgtttt tctaaaggcg
     1021 gcgttgcctg agagtttttt aagtatctta tcgtaaccta tgcttttggg aagtttctgg
     1081 gaagatgtac gtggataaga agtgtaaccc tctgaataaa ggttctgggc tatttgctgg
     1141 gtccttttag ggctgaaacc gaatagggag taggcctctg actgcaggga ccccagatcg
     1201 aaggggaatg gtgggatctt tttggaagtt ttaagttcga tcttggaaac ctgggcgtct
     1261 ttatccttac aatctgccag gatttcatct acatcctctc ttttgaagat cttacccttc
     1321 ttatggtcgg ctatgatgtc tccttccaga tatgctttga tgttccagta gggttctggg
     1381 ataaatgtgc cgatttcctt ctcccggtcg accagtatgg ccagtgcagg tgtctgtact
//
LOCUS       DAOQVW010000452         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_124981_length_1438_cov_2.670282, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000452 DAOQVW010000000
VERSION     DAOQVW010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124981_length_1438_cov_2.670282"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKK85_08555"
     CDS             <1..329
                     /locus_tag="PKK85_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2096 family protein"
                     /protein_id="HNS26104.1"
                     /translation="EALMALAECKGEEYSDSLLDKLRRAAQGEEVYPPKEDRSKFVVG
                     APPGFSMIRVNFKIPLAEDRVQEIAEYHNVIIEFEDDRLVAVYGDPKNLKLSLKELGT
                     FFKEQI"
     gene            405..1094
                     /locus_tag="PKK85_08560"
     CDS             405..1094
                     /locus_tag="PKK85_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS26105.1"
                     /translation="MKILAVTDMHGEFQPLVNYLKSNPVDLVILAGDITHFGPRKLGE
                     DILNEICSYDVPVMAIPGNCDPEFIHGEIESSRAVNIHARCLVVKNVGICGFGGSNPT
                     PFNTPLEFEEVEIYDEAKKALEGIKDHEVTIFVTHTPPHGTSTDLLPSGEHAGSTSLR
                     RLIEEFQPTINICGHIHESRGTDRIRETQVLNPGKLDEGYGCLIHIPEEEKTRIKTKI
                     IALDSNKSKKQ"
     gene            1207..>1438
                     /locus_tag="PKK85_08565"
     CDS             1207..>1438
                     /locus_tag="PKK85_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877485.1"
                     /note="catalyzes the formation of 2-phospho-D-glyceroyl
                     phosphate from ATP and 2-phospho-D-glycerate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HNS26106.1"
                     /translation="MIMVEGEVGGKKYREPFSKGVLARSLTRAEMDPNKAYTFASQIE
                     AHLKEEGVEVIVLDDLIKKVTEKLWEEDQEIAS"
BASE COUNT          425 a          323 c          368 g          322 t
ORIGIN      
        1 aggaagctct gatggcccta gctgagtgta aaggggaaga atactcggat agcttgctgg
       61 ataaacttag acgagcagct cagggtgaag aagtttatcc ccccaaggag gaccggtcca
      121 aatttgtggt aggcgcaccc cctggctttt ccatgatccg ggtgaacttc aagatacccc
      181 tggcagagga ccgggttcag gagatagctg agtaccacaa cgtcatcatc gaatttgaag
      241 acgaccgatt ggtggcggtc tacggtgatc cgaaaaatct taagctgagc ctaaaggaac
      301 tgggcacatt tttcaaggaa cagatataag atttttgctg agtcacctgt ccatgtggga
      361 ccttaaccta cctaagacac aaattcagta gtagaggatg aaaaatgaag atcctggcag
      421 taactgatat gcatggtgaa ttccagcccc tagttaacta tctaaaaagt aacccggtgg
      481 atctggtaat actggccggt gacataaccc actttggccc tcgtaagtta ggggaggata
      541 tcctgaatga gatctgctcc tacgatgtac cagtcatggc catacccggc aactgcgacc
      601 cggagttcat tcatggcgag atcgaaagct cccgtgcagt taacattcat gcccgttgct
      661 tggtggtgaa aaatgtgggc atctgtggat ttggcgggtc caaccccacc cccttcaaca
      721 ctccactgga attcgaggag gtggagatat atgatgaggc taaaaaggcc ctggaaggca
      781 taaaggacca tgaagtaact atttttgtaa cccacacccc tccacatggc accagtacag
      841 acctgctccc ttcgggggag catgccggca gcaccagcct gcgccggctt atagaggagt
      901 ttcagcccac cataaatatc tgtggccata tccacgagtc acggggcaca gatcggatcc
      961 gggagaccca ggtgctgaat ccgggtaaat tggatgaggg ctatggttgc ctcatccaca
     1021 tccccgaaga ggaaaaaacc cggatcaaaa ctaaaataat agccttagat tcgaataaat
     1081 ctaaaaaaca gtaaatctta tccctaaaaa gatgggtaac catttggggt agaaaatgat
     1141 ttaaaggtgg gggatcaacc atcattagat accttttttt aaactttttg aggtgtacat
     1201 atgagtatga taatggtcga aggagaagta gggggtaaaa aataccggga acccttttcg
     1261 aagggggttc tagccagatc tcttaccagg gcggagatgg accctaacaa ggcctacacc
     1321 tttgcctccc agatcgaagc ccacctcaag gaagaaggag tggaggtaat tgtactggat
     1381 gacctcataa aaaaggtcac tgaaaagtta tgggaagagg atcaggaaat agcctcca
//
LOCUS       DAOQVW010000453         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_125114_length_1437_cov_2.920405, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000453 DAOQVW010000000
VERSION     DAOQVW010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125114_length_1437_cov_2.920405"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /gene="hdrC"
                     /locus_tag="PKK85_08570"
     CDS             <1..608
                     /gene="hdrC"
                     /locus_tag="PKK85_08570"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HNS26107.1"
                     /translation="EPEEAVEEPKKERSESMTLLNADENKITRADIDPRFKEEVMEAG
                     AESVAICFQCGTCTGACPSGRRTPYRIRQLVRRAVMGLKNDVISDDSIWMCTTCYECQ
                     ERCPRGIKIVDVVKIVRNYAAQAGYMAPAHKAVGSFVLKTGHGVPINDVTMELRKRVG
                     LDELPPTTHTFPEALEEVQTIIKASGFDKLIGYNWEKGELE"
     gene            618..>1437
                     /gene="hdrB"
                     /locus_tag="PKK85_08575"
     CDS             618..>1437
                     /gene="hdrB"
                     /locus_tag="PKK85_08575"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit B"
                     /protein_id="HNS26108.1"
                     /translation="MAEQEFAYFLGCIMNNRYPGIEKATRIMFDKLGVGLKDMEGASC
                     CPAPGVFGSFDRLTWASIAARNITIAEDQGNDIMTECNGCFGSLYETNHLLHEDPEMK
                     DKVNKVLAEAGREFKGEIQVRHFAEILYNDVGLDKLAEAVTKPLNLNVAVHYGCHFLK
                     PSDAIGIDDPIKPTILDELVEITGAKSVDYKDKMMCCGAGGGLRSRDNDVTADFTREK
                     LTNMRDAGVDAIINVCPFCHLQFDVGQVETNNKFGTDFEIPVFHLAQLYGMAMGV"
BASE COUNT          430 a          345 c          355 g          307 t
ORIGIN      
        1 aagaaccaga agaagctgtt gaagaaccta aaaaggaaag gagtgaaagc atgacgttac
       61 taaatgcgga tgaaaataaa ataacccgtg ctgatataga tccaagattc aaagaagaag
      121 taatggaagc cggtgcagag agcgtagcta tctgcttcca gtgcggtacc tgtactggtg
      181 cctgcccctc aggtaggaga actccctacc ggatccggca actggtacgc cgggcagtta
      241 tgggattgaa aaatgatgtc atatctgatg attccatctg gatgtgcacc acctgctatg
      301 aatgccagga aagatgtcca cggggcatta aaattgtaga tgtagtgaaa atagtacgaa
      361 actacgctgc ccaggccggt tacatggccc ctgctcacaa ggcagtgggg tccttcgttt
      421 taaaaacagg acacggcgtc cccattaacg atgtcaccat ggaactcaga aaacgagtgg
      481 gactggatga actaccacca actacccaca ccttccccga agcattggag gaagttcaga
      541 cgataatcaa ggcttccggt ttcgataaac tcatcggcta caactgggag aaaggagaac
      601 tagaataagg tgataacatg gctgaacaag aatttgccta cttcttagga tgcatcatga
      661 acaaccggta ccccggtatt gaaaaagcaa cccgcatcat gtttgataaa ttgggtgtag
      721 gacttaagga catggaagga gcatcctgct gccccgctcc aggggtgttc ggatccttcg
      781 accgactcac ctgggcttca atagccgccc ggaacattac catagcagaa gatcagggta
      841 acgacatcat gaccgaatgt aacggttgtt ttggttcctt atacgaaacc aaccacctct
      901 tacacgaaga ccctgaaatg aaggataagg tgaacaaagt actggctgaa gcaggccgtg
      961 aattcaaggg cgaaattcaa gtccgacact ttgccgagat cctctacaac gatgtgggcc
     1021 tggacaaact agcagaagca gttaccaaac ccttaaatct aaatgtagcc gtgcactatg
     1081 gttgtcactt ccttaaaccc agcgatgcta tcggcataga cgaccccatc aaacccacca
     1141 tcctggatga acttgtggaa atcaccgggg ccaaatccgt ggactacaag gacaaaatga
     1201 tgtgctgcgg agctggtgga ggtctaagat cccgggataa cgatgtcaca gctgatttca
     1261 ccagggaaaa actcaccaac atgagggatg ctggtgtgga tgccatcatc aatgtctgcc
     1321 ccttctgcca ccttcagttt gacgtgggac aagtggagac caacaacaag ttcggcaccg
     1381 acttcgagat ccctgttttc cacctggccc aactctatgg tatggccatg ggtgtaa
//
LOCUS       DAOQVW010000454         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_125187_length_1437_cov_1.909551, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000454 DAOQVW010000000
VERSION     DAOQVW010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125187_length_1437_cov_1.909551"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKK85_08580"
     CDS             <1..409
                     /locus_tag="PKK85_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNS26109.1"
                     /translation="HEALKIIMDEGLKNRINRHKLAAEATRNGVKALDLELFPEEKVS
                     STTVTAVRMPEGITDGEFRGTMRNKYRVELAGGQDHLKGKIFRIGHMGNITHRELITT
                     FSAMEMTLRELGFDIEMGEGVAAVADTYLPEDV"
     gene            complement(499..>1437)
                     /locus_tag="PKK85_08585"
     CDS             complement(499..>1437)
                     /locus_tag="PKK85_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS26110.1"
                     /translation="GDLKEVQGIAYGDEVTRPRPVVNDLDQLPFPARHLLPMEHYKIL
                     NMKLHTATMISGRGCPMQCSFCASAALHGRRLRMRSAENVVDEMEHLTEEHDAGMNAF
                     MDDTFTLKPKRVEQVCDEIKRRGLDVYWGCTARADTLSSRMLQKMSDTGCITMFLGVE
                     SADQQQLDRVNKQLTVDKIRQAFALSRKHNIRTIASVVLGMPGDTQGSINRTIRFVRD
                     LNPSYALFSLATPYPGTKFYREAVENNLLKVKDWSKYTLLSPVLETVDCSREELKNMQ
                     KKAFRQFYLRPSYILRQLRMDGPIIIKTIAAMIREV"
BASE COUNT          323 a          375 c          393 g          346 t
ORIGIN      
        1 gcacgaggca ctgaagatca tcatggacga gggacttaaa aaccggatta accggcacaa
       61 attagcagcc gaggccaccc gtaatggtgt taaagccctg gacctggaac ttttccctga
      121 agaaaaggtt tcatccacca ctgtcaccgc ggtacggatg ccggagggaa ttaccgacgg
      181 cgaattccgg ggcaccatgc gtaacaagta ccgggtggaa ctggctggag gccaggacca
      241 cctcaagggt aagatcttcc gtatcggcca catgggtaac atcacccacc gggaactcat
      301 aacaaccttc tcagccatgg agatgaccct gcgggagcta ggcttcgaca tcgaaatggg
      361 ggaaggggtg gctgccgtgg cagacaccta cctccccgag gacgtataag tcctcattat
      421 ttttttattt tatccaggga agggatattc atccaattat ttacatctaa aaaaaaatat
      481 tgaaggttaa ctctcctttt acacttccct gatcatggcg gcgatggttt tgattatgat
      541 gggtccatcc atccgtagct gcctcagaat gtaggagggc cgcaggtaga actgccggaa
      601 ggcctttttt tgcatattct taagctcctc cctggagcag tccacggttt ccagcaccgg
      661 agagaggagg gtgtacttgg accagtcctt caccttgagg aggttgttct ccactgcctc
      721 ccggtagaat ttggtgccgg ggtagggtgt ggccagtgag aacagggcgt aggatgggtt
      781 cagatcccgt acgaatcgga tggtccggtt gatactgccc tgagtgtcgc cgggcatgcc
      841 cagaaccaca gaggctatag tgcggatgtt atgttttcgg gagagggcga aggcctggcg
      901 tatcttatcc acggtgagct gcttgttcac ccggtccagt tgctgctgat ccgctgattc
      961 cacccctaaa aacatggtga tacaaccagt gtcactcatc ttctggagca tcctgctgga
     1021 tagggtgtcc gcccgggcag tgcaccccca gtaaacgtcc agtcccctcc ttttgatttc
     1081 gtcacagacc tgttccaccc gcttgggttt cagggtgaag gtatcatcca tgaaggcgtt
     1141 catcccggca tcatgttctt cggtgaggtg ttccatctca tccaccacat tctctgcaga
     1201 ccgcattcgt aaccgtcgac catgtaaagc agccgaggca cagaaggagc actgcatagg
     1261 gcatcccctt ccagagatca tggtggcggt gtggagtttc atattcagta ttttataatg
     1321 ttccatgggc aggaggtgcc tggccgggaa tggtagctgg tccaggtcat tcaccactgg
     1381 acggggtcgg gttacctcat ccccataggc tataccctgg acttctttga ggtctcc
//
LOCUS       DAOQVW010000455         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_125949_length_1432_cov_2.066086, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000455 DAOQVW010000000
VERSION     DAOQVW010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125949_length_1432_cov_2.066086"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKK85_08590"
     CDS             complement(<1..243)
                     /locus_tag="PKK85_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNS26111.1"
                     /translation="MTLIENLFQDIPIHPLLVDVIGISIILILAFVSMRIINYFLEKT
                     GKHLDLELTVIQVLEEIFKYTIIFIAFVLVLHELGID"
     gene            complement(240..1142)
                     /gene="rnz"
                     /locus_tag="PKK85_08595"
     CDS             complement(240..1142)
                     /gene="rnz"
                     /locus_tag="PKK85_08595"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNS26112.1"
                     /translation="MEITFLGTSSALPTKNRSHPAITLKAFGEIILLDCGEGTQRQMT
                     LAGISPMKINHIFITHLHGDHFLGLPGLIQSMAFRGREKPLHIYGPRPIGQLMENIVD
                     LGYYSINFTINTYEVESGLILDEEDFKVYSFPAEHNVPNLAYKIEEKRSPKFLKDKAM
                     ELGVKPGPDFSRLQAGNPVKVGDKIIRPEQVLGDERKGRQIVYSGDTRPCTSMVDFAR
                     GVDVLIHEATFQEALRERAVETCHSTAQDAAKIAKDAGVDNLILTHINTRYQDTDQLE
                     KEGLNMFKNLIIARDFLSFEVKRI"
BASE COUNT          389 a          287 c          310 g          446 t
ORIGIN      
        1 atctataccc agctcatgga gaaccagtac aaaggcgatg aagattatag tatacttgaa
       61 aatttcctct aacacctgta tgactgttaa ttccagatca aggtgttttc ctgttttttc
      121 caggaagtag ttgatgatcc tcatggatac gaacgccagg atcaagatga tggaaattcc
      181 gataacatcc accagaaggg gatggatggg tatatcttgg aaaagatttt ctatcaaggt
      241 catatccgtt tcacctcaaa ggataggaag tccctggcta ttattaagtt tttaaacata
      301 tttaatcctt ctttttccaa ctggtcggtg tcctggtatc tggtgttgat atgggtgagt
      361 attaaattat ccacccctgc atccttcgct attttggccg catcttgagc ggtggaatga
      421 caggtctcaa ccgccctctc tctaagggct tcctggaagg tagcttcatg tatcaggaca
      481 tccacgccac gggcgaaatc aaccatactg gtgcagggcc tggtatctcc agaatagact
      541 atctgccttc cctttctctc atctcccagg acctgttctg gtctgataat tttatctcct
      601 accttcaccg ggttacctgc ctgcaaccgg ctgaaatcag gtccaggttt cactcccagt
      661 tccattgcct tatcctttaa gaatttcggt gagcgttttt cctctatctt gtaggcgagg
      721 ttggggacgt tgtgctcggc ggggaacgag tagactttaa agtcttcttc atcgagtatc
      781 agtcccgatt ccacctcata ggtgttgatg gtgaaattta tggagtagta tcctagatcc
      841 acgatgttct ccattaactg tcctatgggc cgtggtccgt atatgtgcag gggtttctcc
      901 cttccacgga atgccatgga ctgtatcagt ccgggtaatc ccaggaaatg gtcaccgtgg
      961 aggtgggtta tgaatatgtg gttgatcttc atgggactta tcccggccag ggtcatctga
     1021 cgctgggtgc cctcaccaca gtccaggagg atgatttcac caaaagcttt caaagttatt
     1081 gctgggtggc tcctgttttt tgtgggtagt gctgatgagg ttcccaggaa tgttatttcc
     1141 attgatttca cctataaatc tagttaaact ggttttattt aaatgttttt gctatctgaa
     1201 tcgaggatta accaaattaa tttattatat gttaaaaaag aaatataaag tgaattaaat
     1261 ttacaactat aaaaacatgc aatttaaacg tttacaatca caagtgatta aaaataagga
     1321 tcattgaaat aaaaaaatta aggatcattt gagattaaaa aagggatcat tgaaaattta
     1381 aaaaagggtt catcgaatgg tgatcatata tgaatatatc aaacaggtgg ta
//
LOCUS       DAOQVW010000456         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_126295_length_1429_cov_3.182678, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000456 DAOQVW010000000
VERSION     DAOQVW010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126295_length_1429_cov_3.182678"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..1090)
                     /locus_tag="PKK85_08600"
     CDS             complement(209..1090)
                     /locus_tag="PKK85_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS26113.1"
                     /translation="MLTSVQKEILQSLTNLYRKADGKSIKGEEIAEVMNRNPGTIRNQ
                     MQSLRSLGLVKGVPGPRGGYKPTVKAYQTLNIDESDKQTLVPIYKDGKKLKDLSVTKI
                     EFTSITHPGECEAAIKAVGNIKHLDLGDKVRIGPTPVNKLVVDGEVVGRDDMDNILLL
                     DTTAIRSIPKKKVIEVATKKLTTLDISKQIREAALLLSEREIEGAPVNRDGEIVGMLT
                     LSDISQALAEGKEDLKVADIMSTDIITVEEEVMIAEAIEIMNRNHIGRLIVVDENGKS
                     LGIVTRTDLLDKIAGIK"
BASE COUNT          417 a          300 c          215 g          497 t
ORIGIN      
        1 aactttgtgg cacggtgaat gcgtcccgct cctaaaaccc ctgccttccc catctcctcc
       61 agaagttcat ttatactcat tccttcccta ataatcattt gttgaacttt caaattaaat
      121 cacgacctca aatgggctgt ttaaaatcag aagtattatt actccttgat aaaaatattt
      181 gagtaattaa ttacatgaaa aatatattct attttattcc ggcaatcttg tccagaagat
      241 cagtgcgagt gactattcca agggatttac cattctcatc tactacaata agcctgccaa
      301 tgtgatttcg gttcatgatt tcaattgctt cagcaatcat aacctcttct tctacagtga
      361 taatgtcagt ggacataata tcagctactt taagatcctc tttaccttca gccagggctt
      421 gggagatgtc actgagcgtc aacattccca ctatctctcc atccctatta accggagcac
      481 cctcaatttc ccgttccgaa agtaacaaag cagcttctcg gatctgtttt gatatatcga
      541 gtgtagttaa ttttttcgtt gcaacttcta taaccttttt tttaggtatg ctccttatgg
      601 cagtggtgtc cagaagtagg atgttatcca tatcatctct acccaccact tcaccatcta
      661 ccaccagttt gtttacagga gtggggccaa tacgaacctt atctcctaaa tctaagtgtt
      721 ttatattacc cactgcctta atagctgctt cacattcccc gggatgggtg atgctggtga
      781 actcgatctt ggttacagaa agatctttaa gttttttccc atccttataa atgggaacta
      841 gagtttgttt atctgattca tcaatattaa gggtttgata tgcctttaca gtaggtttgt
      901 atcctccacg tggaccagga actcctttaa ctaaacctaa acttctaagg gattgcatct
      961 gattacgaat agtaccaggg ttacggttca ttacctctgc tatttcttct cccttaatgg
     1021 acttaccatc ggctttgcgg tataaattag taagactttg caggatttcc ttctggactg
     1081 aagttaacat aaaatacccc tacttatttg attataaaat atttatcatt ataaatcaat
     1141 ataaatgtta tgatttagat taatttgatt actatttaac ccaattaatg taaatgtcaa
     1201 ccgttatgta acaaattcat ctttttcggc aattattttt ggattattac gctttaaata
     1261 tttgaaaatt attaatcaac catcatctat caccctcgaa gatggaaatt ttgaaaatcg
     1321 gaattcaaca atgctattac accatgccgg ctaatccaca tattgacata tctatctttc
     1381 ttttataagg ttaatgataa aaaaaattaa aaaatacgcc ttttatggg
//
LOCUS       DAOQVW010000457         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_127026_length_1424_cov_2.902118, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000457 DAOQVW010000000
VERSION     DAOQVW010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127026_length_1424_cov_2.902118"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKK85_08605"
     CDS             complement(<1..654)
                     /locus_tag="PKK85_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNS26114.1"
                     /translation="MSLEFNLRSKVYWKRMASGIFLGFCSAIGAFIFIVLVHLGQKVF
                     LPYDFNNWAPFTGPWWIVALMTVAGLLVGVIHRYTSAQQLDAFKALDEGCLDPEPVPP
                     SLLTSFISLLGGFSLGPEVPCGFLAAGLATWFSNKWGMDPDTTRINVASSVSGAYGGI
                     FSSPFAMILILLESKHRQSVQFYGTLFIAGMAALVGFIIFYTLQGFNYASLLGLLTPP
                     "
     gene            complement(842..1057)
                     /locus_tag="PKK85_08610"
     CDS             complement(842..1057)
                     /locus_tag="PKK85_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNS26115.1"
                     /translation="MPTVHVNVWKGFKEEDVKFVIENITQVFVDLDIPAHAVEVLVHE
                     VPQSHWGIGGVPASEKFKDLKIPGWDQ"
     gene            1299..>1424
                     /locus_tag="PKK85_08615"
     CDS             1299..>1424
                     /locus_tag="PKK85_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNS26116.1"
                     /translation="MYTEEGEKLKKLLDLENEPVAIKWSVREPRNIKKEEGKSRFC"
BASE COUNT          418 a          303 c          359 g          344 t
ORIGIN      
        1 agggggagtt agaaggccca gtaggctggc ataattaaag ccctgcaggg tgtagaagat
       61 gatgaaacca acaagtgccg ccatccctgc gataaagagg gtgccgtaga actgaaccga
      121 ctggcgatgt ttggattcta agaggatgag tatcatggca aagggtgagg agaatatccc
      181 accataggca ccgcttacac tgcttgccac gttgatccgg gtggtgtccg ggtccatgcc
      241 ccatttattg gagaaccagg tggccagccc cgctgccaga aagccgcagg gcacctctgg
      301 tcccaggctg aaaccaccca ggagggatat gaaggaggtg agaagggatg ggggcacggg
      361 ttcaggatcc aggcatccct catccagggc tttaaaggca tccaactgct gggcagaggt
      421 gtaacgatgg ataaccccca ccaggaggcc ggctacagtc attaaggcca caatccacca
      481 tggccctgtg aagggcgccc agttgttaaa atcatagggc aggaaaacct tctggcccag
      541 gtgcaccagt acaatgaaaa taaaggcccc tatggcgctg caaaatccta gaaaaatgcc
      601 agaggccatc ctcttccagt acacttttga tcggaggtta aattcaaggg acataaaatc
      661 ttactccacc agtggtttat cctggaaatc taattaaaac taaactggct gattataaag
      721 ttatctttgc tgtgataaaa agtttcacct ggacaaaaga aaaaggaagt tacaaggggg
      781 tataatgtat ctgttatccc ctgggctaac atcccagcta gtggatgcct ttaataaagc
      841 ttcactggtc ccagcctggt attttaagat ctttaaattt ctcggatgct ggtactccgc
      901 caatacccca gtgggattgg ggtacttcgt gtactaaaac ttccactgca tgggctggaa
      961 tatccagatc cacaaagacc tgggttatat tctcaatcac aaatttcaca tcttcctctt
     1021 tgaagccttt ccagacgttt acatggaccg ttggcatttt atcaccatct tctaccttat
     1081 caatatatat tgtccttgga gattattatt aatagtagag gagggggtgt ggcttgagga
     1141 aaatatctga cacaaaactg gtctcttaac tggtactagg gtgatgatac cagagaacta
     1201 ttggatcatt tattcgaaaa ctaaatcatc ccctaggata caattgctta gtagtgatat
     1261 tgtaatggta ctgaaccatc tatgggtagg ggagtgttat gtataccgaa gaaggggaaa
     1321 agttaaaaaa actgttggac ttggaaaatg agccggtagc catcaaatgg tcggtgaggg
     1381 agccaagaaa cattaaaaag gaagagggaa agtcgaggtt ctgc
//
LOCUS       DAOQVW010000458         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_128267_length_1416_cov_2.795738, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000458 DAOQVW010000000
VERSION     DAOQVW010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128267_length_1416_cov_2.795738"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1416
                     /locus_tag="PKK85_08620"
     CDS             159..>1416
                     /locus_tag="PKK85_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNS26117.1"
                     /translation="MTSTPVVIPQGGKFMSTKLIPELLAPAGSISALKAAAATGADAV
                     YLAGSNFGARHYAENFSRKEMEEAFNYAHLRGLKVYVTVNTLQKDAELVEVADYLCWL
                     YERGADGVIVQDLGVAGLARDLVPKLPLHASTQMTIHNQEGVKWAADFGFKRVILPRE
                     MGLGEIKQMSQELIKGVELEVFIHGALCYSYSGQCLLSSLIGGRSGNRGMCAQPCRKT
                     YQLVTGPTDQYGRPLGLTEVSMEGDYLLSTRDLSLYPYLEEVVNSPISSFKIEGRMRS
                     PVYVALVVSIYRKALEAIKNGNWKPQKEDIENLKLAFNRGFTRGHILEARPGSTMERR
                     SPGNRGLYLGQITHWDPVSHRATLKLESSSFPQQGDGLLFTTQSGKKVVGMGLDKTPP
                     LHQGRVIIKPPRMVQPGYKVYMTRKES"
BASE COUNT          388 a          350 c          345 g          333 t
ORIGIN      
        1 tcccatatcc ccccttataa ctgcgttgta ccataccgaa gaatttcgac ctatatgaac
       61 atcacccaca attttcgcgc cagataattg tttaaccgtc ctatgaatca taaaaaccag
      121 cccctatcaa taatatatga acccggggac agataataat gactagcact ccagtagtca
      181 taccccaggg agggaaattc atgtccacca agttaatacc agaactttta gcaccagcag
      241 gttccatatc cgcccttaaa gcagcagctg cgactggtgc cgatgccgtg tacttggctg
      301 gttcgaattt cggggcccga cattatgctg aaaatttcag ccgcaaagaa atggaggaag
      361 catttaatta cgcccattta aggggtttaa aggtttatgt aacggttaac accctccaga
      421 aggatgctga actagttgaa gtggctgatt atctgtgctg gctttatgaa agaggagctg
      481 atggggttat tgtccaggat ctgggggtgg ctggcttagc ccgggatctg gttcccaaac
      541 ttcccctcca cgcctccacc cagatgacca tccacaacca ggaaggagtg aaatgggccg
      601 ctgattttgg atttaaaagg gttattctcc cacgagaaat gggactaggt gaaataaagc
      661 agatgtccca ggaacttata aaaggggtgg agctggaagt cttcatacat ggagcactat
      721 gctactccta ttcgggccag tgtcttttat catccctgat aggaggtaga agcggtaacc
      781 ggggcatgtg cgcccagccc tgccgtaaaa cttaccagtt ggtcactggc ccgacggacc
      841 agtatgggag gccactagga cttacggaag tgtctatgga aggagattac ctactctcca
      901 cccgggatct ctccctatac ccctacctgg aggaagtggt taattctcct atttcttctt
      961 ttaagattga aggccgaatg cgttccccag tatacgttgc cctggtggta agcatatacc
     1021 gtaaagccct ggaagctatt aaaaatggga attggaagcc ccaaaaagaa gatattgaaa
     1081 atttaaagct ggccttcaac cggggattca cccggggcca catacttgaa gcaagacctg
     1141 ggagtaccat ggaaaggcgg agccctggaa acaggggcct atatctgggg cagatcaccc
     1201 actgggatcc tgtttcccat cgggccacac tgaaactgga aagttccagc tttcctcaac
     1261 agggggatgg gctgcttttc accacccagt ccgggaaaaa ggtggtgggt atgggccttg
     1321 ataaaacacc ccccctacac cagggccggg ttattataaa acccccccgc atggtgcaac
     1381 ctggatataa agtatatatg acccgtaaag aatccc
//
LOCUS       DAOQVW010000459         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_128632_length_1414_cov_2.109639, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000459 DAOQVW010000000
VERSION     DAOQVW010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128632_length_1414_cov_2.109639"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PKK85_08625"
     CDS             complement(<1..241)
                     /locus_tag="PKK85_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNS26118.1"
                     /translation="MTPLVLIIPMYILLYYNFNLYDPQRTRQDIGSEAFQIIKVNLVG
                     VLVLATVLFVLELTDYSRYLLAMFAIFSIVFSILER"
     assembly_gap    290..299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(417..1292)
                     /locus_tag="PKK85_08630"
     CDS             complement(417..1292)
                     /locus_tag="PKK85_08630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012954920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS26119.1"
                     /translation="MPDLSIIIVNYRTYQLTRDTLESVLEGDHPFTYHIYLVDNHSMD
                     GSLEKLKQDYSSHEHITFLESEANLGFAHANNLALKIADSSYVLLLNSDTQILGDCLE
                     QCLDCMDHDKEIGILGCKVILPDGSLDLACRRSFPTPMVSFYRMSGLSRLFPRSRRFG
                     RYNLTYLDEDGSYPVDCVVGAYMMVRWETVEEEGDLDEDFFMYGEDVDWCYRVKSAGW
                     KVFYLGSARILHYKGGSGASEKLLKEFYHSMQLFYKKHYELMYPWYVNALTYGGIRGI
                     YGLKLFFLKIRKFFK"
BASE COUNT          353 a          331 c          352 g          368 t
ORIGIN      
        1 aacgttctag tatggaaaag acgatgctga agatggcgaa catggccagc aggtaccggg
       61 agtagtcggt taactccagt acaaagagca ccgttgccag taccagaacc cccaccaggt
      121 tgacctttat gatctggaag gcctcggatc ctatgtcctg cctggttctc tggggatcgt
      181 agaggttgaa attatagtac aagaggatgt acatgggtat aatcagtaca agtggggtca
      241 tgtaatggta aaatccccat tggctgctgc caaagcccag cagatcagtn nnnnnnnnnt
      301 aaccgccaca tcgatgagga tgaggatcag gttaaagaat ctctggtact gtttgatcat
      361 gtaaagggca ctccagatag gtatgggaca ggctttttac tttttatatt ggcaaactac
      421 ttaaaaaact ttcttatttt taaaaagaaa agtttcaacc cgtagatacc cctgatcccc
      481 ccataggtca gggcattaac ataccaggga tacattaact cgtagtgttt cttataaaaa
      541 agctgcatgg agtggtagaa ttcctttaag agcttttcac tggctccact accaccctta
      601 tagtggagga tccgggctga gccgaggtag aaaaccttcc accccgcgga tttaacccgg
      661 tagcaccagt caacatcctc cccgtacatg aagaaatcct catccagatc tccctcctcc
      721 tcaacggtct cccaccggac catcatatag gctcccacca cacagtccac agggtagctt
      781 ccatcctcat ccaggtaggt gaggttgtag cgtccgaagc gacggctccg ggggaataat
      841 ctggaaaggc cactcatccg gtagaatgat accatggggg tggggaagct tctccggcag
      901 gccaggtcca ggctgccatc gggcaggatc accttacaac ccagtatccc gatctccttg
      961 tcatggtcca tgcagtccag gcactgttcc aggcagtccc ccaggatctg ggtgtcggag
     1021 tttaaaagca gcacgtaact ggagtcagca attttcaggg ccaggttgtt ggcatgggca
     1081 aagcccagat tggcttcgct ttccaggaag gtgatgtgtt catggcttga atagtcctgt
     1141 tttagcttct ccaggctgcc atccatggag tggttgtcca ccaggtagat gtggtaagtg
     1201 aagggatggt caccttccag cactgactcc agggtatctc tggttagctg gtaggtgcgg
     1261 tagttgacta tgattatgga taggtctggc atggcttttt tcccatcatc ttgatctaga
     1321 tttcattttc tatcttttat ggattagcta gctacaggaa gagataaagt agcgtgtttc
     1381 tggtaagcat ccactggatt ttgatgtagt tttt
//
LOCUS       DAOQVW010000460         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_128856_length_1412_cov_2.984525, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000460 DAOQVW010000000
VERSION     DAOQVW010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128856_length_1412_cov_2.984525"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /gene="dnaX"
                     /locus_tag="PKK85_08635"
     CDS             complement(<1..862)
                     /gene="dnaX"
                     /locus_tag="PKK85_08635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNS26120.1"
                     /translation="MTQYQSIYRKWRPQSFEDIVGQNHITQTLKNAIKMNRIAHAYLF
                     SGPRGVGKTTTARIFAKALNCEKGPTETPCGQCSRCIRISQGQSLDVLEIDGASNRGI
                     EEIRELRSKIGFAPAEGKYKIYIIDEVHMLTDPAFNALLKTLEEPPSQVLFIFATTAP
                     YKVPNTILSRCQSFNFRRISIEEMVEKLKKITDEEKINIDSSSLRLIAESATGSMRDA
                     ESILDQVIAYSENKVTAQEVRDILGLLPHEIFYQFFDNIINHNTETALKLINKLVKEG
                     VELNKFVQDLI"
     gene            complement(910..1173)
                     /gene="ffs"
                     /locus_tag="PKK85_08640"
     ncRNA           complement(910..1173)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKK85_08640"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          395 a          315 c          250 g          452 t
ORIGIN      
        1 taatcaaatc ctgaacaaat ttattcaact ccactccctc tttgaccagc ttatttatca
       61 atttaagcgc tgtttcagta ttatggttaa taatattatc aaaaaactgg taaaatattt
      121 catggggaag gagtcccaga atatccctga cttcctgggc tgttacctta ttttcactat
      181 aggctataac ctgatctaaa atactttctg catctctcat agaaccggta gcgctctctg
      241 ctattaaccg tagagaggac gagtcaatgt ttattttttc ttcatcagta atttttttta
      301 atttttccac catttcttca atggaaattc tacgaaaatt aaaagattgg cagcgtgaga
      361 gaatagtatt gggaacctta tatggtgcag tagtggcaaa aataaataaa acctgactgg
      421 gtggttcttc taaagtcttg agtaaagcat taaaggcggg atcggtcagc atatgtactt
      481 cgtcgatgat atatatttta tacttgcctt ccgccggtgc aaatccaatt ttacttctta
      541 actctctgat ttcttcaatt ccccggtttg aagccccatc tatttctaac acatctaaag
      601 actgcccctg gctgatacga atacatcggg agcactgccc acagggtgtt tccgtgggtc
      661 ccttttcaca gttaagcgcc ttggcaaaaa ttcgggcagt agttgttttt cccactccac
      721 ggggaccgga gaaaagatag gcatgggcaa tgcgattcat cttgatagca ttttttaggg
      781 tttgtgtgat gtgattctgg cctacaatat cttcaaaaga ctgaggtctc cattttctat
      841 agatagattg atattgagtc atatatggtt tccctcacaa tagtgatata ctccttatta
      901 aaaagacgac cgtgccctac cctcgaacca ccttcccaag cgataatgaa acagttaact
      961 ccaatcaggc ttccctgcgg cacccaaaga gatttactta ccgctgcttc cttccagacc
     1021 tgacggggtt tgtaattctt tgctgcacag gacccaatct tcaacatcac ttattccacc
     1081 cggaccttaa aaagataagc cctcaaatag gttttcgatc ctgctctagc ggattgcagg
     1141 ttcagggcac cgctaactcc ccacctagca cggtcatcac ttaaaagcat atcataaagg
     1201 ggaatccttc ccctttagaa cccctgaaag actttcttcc acttaaggat gtaatttgta
     1261 tatattttgt tgtatttaga ctgaagttca taatctcagg tgatatatgt cttcagccta
     1321 taaaggggaa tccttcccct ttaaaacccc tgaaatacag ttcttaacta ataatgcagt
     1381 tgatcattat tttttatttt aattatcctg ag
//
LOCUS       DAOQVW010000461         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_130262_length_1403_cov_3.196588, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000461 DAOQVW010000000
VERSION     DAOQVW010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130262_length_1403_cov_3.196588"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKK85_08645"
     CDS             complement(<1..446)
                     /locus_tag="PKK85_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNS26121.1"
                     /translation="MARYRCTVCNYIFDEEREGIKFEDLPENWRCPVCNSPLSTFVLL
                     SASLEYKDVDSTVSEVMVDQMAEWGIQFVFGIPGTSSLGVVDAIRQEDRIKYIQVRHE
                     QTAAMMASAYGKLTGHLAACLTVAGPGATNLATGLYDAKLDHAPVLA"
     gene            597..1361
                     /locus_tag="PKK85_08650"
     CDS             597..1361
                     /locus_tag="PKK85_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26122.1"
                     /translation="MGNFKIHCRDENEKFIPTRSYQELLRHFSKFKKQHGIILHVVGA
                     PGIGKSTNIYQASQEIGLKVYEACLILNSEKMSPKVVFDLTLDFMKKDLGIKTDLALY
                     RKLKEFDMVLFADRFHDYHRIDPATVGFSQWTANKGWSAVPYYWYCMKEFLKQKNEFR
                     NLNIVFQTSWSVHLGGRKRDLFTELGLISRISLFLLKIPFQVVEISYSPLETITIVKS
                     HFHNVDEDRIKKKIALYNCKPRFICEALKTGNNKYG"
BASE COUNT          451 a          259 c          269 g          424 t
ORIGIN      
        1 gccagcactg gggcatggtc taatttggca tcgtagagtc cagttgccag attagtggct
       61 cctggaccgg ctactgttaa acaagctgcc aaatgtccag ttaatttacc ataggcagag
      121 gccatcatgg cggcagtctg ttcatgacgc acctgtatgt acttaatccg gtcttcttgg
      181 cggatagcat ccaccacacc cagtgaagaa gtacctggga tgccgaacac aaattgaata
      241 ccccattctg ccatctgatc caccataact tcagaaaccg tggaatccac atctttgtat
      301 tctaaactag cactcaaaag tacgaaggtt gatagtggtg aattgcaaac agggcatcgc
      361 cagttttcgg gcaaatcttc aaatttaatt ccttcccgtt cttcatcaaa gatgtaatta
      421 cataccgtgc atctgtacct ggccattgca atcccctctc atcaatctat aacttatata
      481 tctattggtt ttgaaactaa taaagacatc ttttggcaaa aaaaaacaat gatttaagat
      541 aatttagaga ttttaagaat ttttttattt atatagccta aaataaaagg taagttatgg
      601 ggaatttcaa gattcactgt cgggatgaaa atgaaaagtt catccccact cgatcctacc
      661 aagaactact aaggcacttt tcaaagttta aaaaacagca tggcataata ttgcatgtag
      721 ttggtgctcc agggatagga aaatctacta atatttacca agcctctcaa gagataggtt
      781 tgaaagttta tgaggcttgt ttaattctga atagcgaaaa aatgtcgccg aaagtagtgt
      841 ttgaccttac tctggatttt atgaaaaaag atctgggtat taaaactgat ctggcacttt
      901 atcgtaagct aaaagaattt gatatggtat tatttgctga tagattccat gactatcatc
      961 gtatagatcc tgctacagtt ggattcagcc agtggactgc taataaggga tggagtgcag
     1021 ttccatatta ttggtactgc atgaaagaat tcttgaaaca aaaaaatgag tttagaaact
     1081 taaatattgt tttccagaca tcatggagtg tccatttagg tgggaggaaa cgagaccttt
     1141 tcacggagct gggccttatt tccagaattt cactttttct tttaaaaatt ccatttcaag
     1201 tggtggagat atcttattcc ccattagaaa ctataactat tgtgaaaagc cattttcata
     1261 atgttgatga ggatagaatt aagaaaaaaa ttgcactcta caattgcaag cccagattta
     1321 tatgtgaagc ccttaaaact gggaataata agtatggata gggaagtaaa tgaatgatgt
     1381 ccttttaacc cttctcaaat cca
//
LOCUS       DAOQVW010000462         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_130492_length_1402_cov_2.434298, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000462 DAOQVW010000000
VERSION     DAOQVW010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130492_length_1402_cov_2.434298"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PKK85_08655"
     CDS             <1..394
                     /locus_tag="PKK85_08655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNS26123.1"
                     /translation="YWLIFLAPSQRYLGTCVVAQKKGCKDLSEVETKEWVEFGLIVKK
                     LEESLRTIFNPTLFNWSCFKNATFRGPNPKPEVHWHFIPRYKEEVSFGGLKFKDPDFG
                     YIPQPISRELPPDVQKNLKKKIETQLFK"
     gene            472..>1402
                     /locus_tag="PKK85_08660"
     CDS             472..>1402
                     /locus_tag="PKK85_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNS26124.1"
                     /translation="MEISEIVNQMHEEGIESLLIFKPENITYLSGYWPTSTSILLLKD
                     DPVLLVPKLDLKEAEEISALEVDELRSLPKLKKTLPVHLGVEPSLDIATYKKICEEFK
                     IQVTDLVERSRIIKSEGEVHNIQKAIEIAEKSFLEVEMRGSEDEAAARLEYIMRLNGS
                     SKPAFNTIMASGRRSSFPHASTSPENIESPLVIDWGAVYNHYASDTSRTLVETEKEEE
                     IFDIVLEAQEKAIKNIKPGVKASYIDKVARDIITDYGYGESFLHSTGHGVGLEVHEMP
                     SLSSKEDKKLEAGMVITVEPGIYVEGEFGVRVED"
BASE COUNT          468 a          271 c          312 g          351 t
ORIGIN      
        1 atattggctt attttcctgg cgcccagcca gagatatctg ggaacctgtg tggttgccca
       61 aaagaaaggt tgtaaagacc tctccgaagt tgaaacaaag gaatgggtgg aatttggctt
      121 aattgtgaaa aaactggaag aaagtttgcg aacaatattc aaccccacac tttttaactg
      181 gagctgtttt aaaaacgcta cttttcgtgg gccaaatccc aagccagaag ttcactggca
      241 tttcatcccc cgctacaagg aagaagtctc gtttggggga ttgaagttca aagaccctga
      301 ttttggttac atcccccagc ccataagccg agaactccct cccgatgtac agaagaatct
      361 taaaaagaaa attgaaacac aactatttaa gtaaatacta agcatctgaa atgtctaaat
      421 ctaagccaat aaaaaatttt gtgagaaata ccctattttc gaggaagatc catggaaatc
      481 tctgaaatag tcaaccagat gcatgaagaa ggaatagaat ctcttctcat cttcaaacca
      541 gaaaatataa cctatttaag tggttactgg ccaaccagta cctcaatttt acttcttaaa
      601 gatgatcccg tgctcctggt tcccaagtta gatttgaagg aagcagaaga aatatctgcc
      661 ctggaagtgg atgaactgag atcactacca aagctaaaaa agacattgcc agtacatctg
      721 ggagtagaac catcccttga tattgccacc tacaagaaga tatgtgaaga gtttaaaatt
      781 caagtcacag atttggtgga aagatccagg attataaagt cagaaggtga agttcacaat
      841 atccagaagg ccatagaaat tgctgaaaaa tcctttctgg aagtggaaat gaggggcagt
      901 gaagatgaag ctgccgccag gctggaatat ataatgaggc tgaatggctc cagtaaacct
      961 gcatttaata ctattatggc gtcagggcgg cgttccagtt ttccccacgc atctacctcc
     1021 ccagaaaata tagaaagtcc attggtgatt gactggggag cagtatataa tcattacgcc
     1081 tctgacacca gccgtaccct ggttgagaca gaaaaagagg aagaaatttt tgatatcgtc
     1141 cttgaagccc aggaaaaagc cataaaaaat attaaaccag gagtcaaggc ttcttacatt
     1201 gacaaagtgg ctcgagatat tataactgac tatggctatg gtgagagttt cctgcattct
     1261 accggccatg gcgtggggct tgaagttcac gaaatgcctt cattatcatc aaaagaggat
     1321 aagaaattgg aagcaggaat ggtgatcact gtggaaccag ggatatatgt ggagggagaa
     1381 tttggagtcc gagtggaaga ta
//
LOCUS       DAOQVW010000463         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_130890_length_1399_cov_3.957589, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000463 DAOQVW010000000
VERSION     DAOQVW010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130890_length_1399_cov_3.957589"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /gene="iorA"
                     /locus_tag="PKK85_08665"
     CDS             complement(<1..>1399)
                     /gene="iorA"
                     /locus_tag="PKK85_08665"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNS26125.1"
                     /translation="EIGDVLHLLAREAGIYFEFSINEKVALEVAAAAASSGVRSFTFM
                     KHVGLNVASDSLMSAVYTGVRAGMVILTADDPSMFSSQNEQDSRHYARLANLPMLEPS
                     NPQEVKDFILEAFQISEAYQIPVLLRTTTRVSHMRGIVKLGSIEEKPDKGFFKKDPER
                     FVPVPATARVMHRKLVDKMERLGHAMEDSALNVEYAQNGNLGIITGSSAYNYCRDVVE
                     EEELPVNILKIGFSHPFPEKKVLKFLQGVDRVLVAEEVDPIMEKEILVIIGKYGLKLE
                     VNGKLDASLPMIYEYTPDIVCESIHNVFGLKNEKLMIQESDQPIPNRPPTLCPGCPHR
                     AAYYGVNKAAENLGLADMIHPTDIGCYTLGVEAPYWSADYLLSMGSSIGTSCGFSKAT
                     TQPVVSFIGDSTFFHAGIPPLINAVHNKNRFVLVILDNRTTAMTGGQPHPGLPVDGLG
                     NEAPEISIPAIVQAVG"
BASE COUNT          319 a          364 c          348 g          368 t
ORIGIN      
        1 cccaccgcct gcactatagc ggggatagat atctccggcg cctcatttcc cagtccatcc
       61 accggcagtc ccgggtgggg ttggccacca gtcatggccg tggtgcggtt gtccaggatc
      121 accagcacga atcggttttt attatgaacc gcgttgatca aaggtggtat ccctgcatgg
      181 aagaatgttg aatcccctat gaaactaacc accggctgtg tagtggcttt tgagaatcca
      241 cagctggttc cgatggatga acccatggat aagaggtaat cagcagacca gtagggagcc
      301 tccaccccca gggtgtaaca gccaatatca gtggggtgaa tcatatcagc aagtcccagg
      361 ttctcggcag ctttattcac cccatagtag gctgccctgt gggggcagcc agggcacagg
      421 gtagggggcc ggttaggaat tggttgatca ctctcctgga tcatcagttt ttcattttta
      481 aggccaaaaa cattgtggat tgattcgcac actatatctg gtgtgtattc atagatcatg
      541 ggtagggatg catctaactt cccgttaacc tctagtttca gcccatattt tcctataatt
      601 accaggattt ccttctccat aatgggatcc acttcctcgg ccaccagcac tctatccaca
      661 ccttgaagaa actttaaaac cttcttttct gggaagggat gtgaaaatcc tattttaaga
      721 atgttaaccg gcaactcttc ttcttccacc acatcccggc agtagttgta ggcgctgctc
      781 ccggttataa tgcctaggtt accgttctgg gcatactcca cattcagggc tgaatcctcc
      841 atagcgtggc ctagtctttc cattttgtcc accagtttgc ggtgcatgac cctggccgtg
      901 gctggtaccg gtacgaaacg ttccggatcc ttcttgaaga agcctttatc gggcttttcc
      961 tcgattgagc caagtttcac aattccccgc atatgggata cccgggtggt ggtgcgtagc
     1021 aataccggta tctggtaggc ttctgatatc tggaaggcct ctagtatgaa gtcttttact
     1081 tcctgagggt tggagggctc cagcattgga agattagcca gtcttgcata gtgacggctg
     1141 tcctgttcat tctgagaact gaacatggag ggatcatcgg ctgttaagat caccataccc
     1201 gcccggaccc cggtgtaaac cgcactcata agggaatctg aggccacatt gaggcccaca
     1261 tgtttcatga aggtgaagga acggactcct gaactagcgg ctgctgcagc cacctccagg
     1321 gccaccttct cgttaatgga gaattcgaag tatataccgg cctcccgggc taggaggtgt
     1381 aaaacatccc caatctctg
//
LOCUS       DAOQVW010000464         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_131001_length_1399_cov_1.680060, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000464 DAOQVW010000000
VERSION     DAOQVW010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131001_length_1399_cov_1.680060"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..359)
                     /locus_tag="PKK85_08670"
     CDS             complement(69..359)
                     /locus_tag="PKK85_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNS26126.1"
                     /translation="MSEELVNKIREELAKIPDPHMGISIAEMGIVGNIEVNEAEKTAK
                     IEIIPTNPGCMSAANIAMQARLAAESLEEIDKAEIIVKGHMMADAICEMVNK"
     gene            complement(562..>1399)
                     /locus_tag="PKK85_08675"
     CDS             complement(562..>1399)
                     /locus_tag="PKK85_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HNS26127.1"
                     /translation="EPLGGVVVVLPCSMRKPYSQSKSHLVFMRVTKGYQEAILTSPFG
                     VCPREMEKTYPIQSYDVSTTGNWSEEEIDVTGECLREYVKDLPVVAHVAGGYRETCER
                     YLENAVYTCKDGKTTSPESMHNLRTALKSYSKIRDKDKNLKKLRSIARYQFNGKDADS
                     LIPDGSRITGRFNRRVIHEGKQIATLLYETGLYSLNLEGGWILRDLGRSWVEIDFDLK
                     TNTLFAPGVVDAHPDILPGDEVVILRQTDVLGVGKTVLSGEEMKKAVKGVAVRVRHRI
                     KG"
BASE COUNT          334 a          351 c          255 g          459 t
ORIGIN      
        1 ggccacctca ggccgtccga tcaggactat aaccacccac cactgcggca aggttccagc
       61 gtcccatttt atttgttcac catttcacat atggcatccg ccatcatgtg tcctttgact
      121 atgatctctg ccttgtcgat ttcttccagg ctttctgctg ctaatctggc ctgcatggct
      181 atgttagctg cactcataca tcccgggttg gtgggtataa tctctatttt agctgttttt
      241 tcagcttcgt tgacttctat gttgcctaca atgcccatct ccgctatact gattcccata
      301 tgtggatctg ggatttttgc taattcttct cttattttat tcactagttc ttctgacata
      361 attattacct caaaagtaaa ttattaacct tctattagtt tgatatcttc atttgaatgg
      421 aattatggtt gtttaacccc cttaaaaagg tccattcctt ttgaataaat tatattaaaa
      481 tttatttcat gattatattt aaacttatgt ttatttataa tttttataca ataacattga
      541 aagaaagtta aaattcttaa tttaaccctt tattctatgc cttaccctga ccgccactcc
      601 tttaactgcc tttttcatct cctcaccaga gagaactgtc tttccaacac ccaacacatc
      661 tgtctgcctt aaaattacca cttcatcccc ggggaggatg tccgggtgtg cgtccaccac
      721 tcccggtgca aacagggtgt tggttttaag gtcgaaatca atttccaccc aggatctccc
      781 cagatccctt aaaatccagc cgccctccag gttcaaagag tagagtcctg tttcatataa
      841 cagtgttgct atctgtttac cttcatggat tacccgccgg ttgaatctgc cggttatccg
      901 ggaaccgtcc ggtatcaaag aatcggcgtc cttaccatta aactggtaac gagcaatgga
      961 tcttaatttt ttcaggtttt tatccttatc cctaatttta gaatagcttt taagggcagt
     1021 tctcaggtta tgcatggact ctggtgaggt tgtcttacca tccttgcagg tgtaaaccgc
     1081 attttccaaa tacctttcac acgtttcccg gtagccacca gccacatggg ctaccaccgg
     1141 caaatcttta acatattccc tgagacattc accagttacg tctatttcct cctctgacca
     1201 gtttccagtg gtggaaacat cataggattg tatgggatag gttttctcca tctcccgggg
     1261 gcataccccg aatggggagg ttaaaatggc ctcctggtaa cctttggtga ccctcatgaa
     1321 aaccaggtgg gacttggact gggaataggg tttcctcata ctgcagggta gcaccaccac
     1381 cactccgccg aggggttca
//
LOCUS       DAOQVW010000465         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_131184_length_1397_cov_3.811475, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000465 DAOQVW010000000
VERSION     DAOQVW010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131184_length_1397_cov_3.811475"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKK85_08680"
     CDS             <1..272
                     /locus_tag="PKK85_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26128.1"
                     /translation="GLKLFEVAEIAAELSPQVIILTDFDQKGNELARRLSEDIQRLGS
                     HPNILFRRKILEMTSRFIKDIESLPRHLDNLASEEFPRGAYWYYH"
     gene            350..>1397
                     /gene="dnaG"
                     /locus_tag="PKK85_08685"
     CDS             350..>1397
                     /gene="dnaG"
                     /locus_tag="PKK85_08685"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HNS26129.1"
                     /translation="MGKEEISTTKYLIHAQINANGIVEKPDVVGAIFGQTEGLLSNDL
                     DLRELQKTGRIGRIKVNINSKAGRSKGEIVIPSSLDRVETAILAASLETINRVGPCEA
                     YIQVNKVEDVRAVKRRKVVDRAKELYKNMMEEVTPESLKMIEEVKEAMRIHEITEFGN
                     EKLPAGPNVVSSDAILVVEGRADVLNLLRYGVKNAVAVEGVSVPKSVAELTKKKTVTA
                     FLDGDRGGDLILKELLQVGELDYVTRAPRGSEVEDLTKDEVMVALRDKIPVEQIYHDL
                     GVKTEKVEKKTPDKIGLLKGILGDLEGSGNGEVLDDSLNIIKEVKVENLYQEINNLQN
                     GAYAVVFDGVVSQRL"
BASE COUNT          447 a          249 c          352 g          349 t
ORIGIN      
        1 caggtcttaa gctcttcgaa gttgcggaaa tcgctgctga attatcacca caagtgatca
       61 ttctcactga ctttgaccaa aagggtaatg aacttgcccg gaggttatct gaagatatac
      121 agcgacttgg ttcacatccc aacatccttt tcaggagaaa gatattagag atgacaagtc
      181 gctttattaa agacatagaa agcttgcccc gtcatctgga caatctggct agtgaagaat
      241 tccctagggg tgcctactgg tattatcatt agtatcgttt agatacctgc ctacattggc
      301 ctgatatcat ttgatatcat cagtatctac tctatcagga ggcccaatta tgggaaaaga
      361 agagatcagt acaactaaat atcttattca tgctcaaata aatgctaacg gaattgttga
      421 aaaacccgac gtagtcggtg ctatttttgg acagacagaa ggacttttaa gtaatgatct
      481 ggatcttcga gaactgcaga aaactggaag aatcggaaga attaaggtca acataaattc
      541 caaggcagga agatccaaag gcgaaatcgt aataccctcc agtctggacc gagtggaaac
      601 agccatcctg gcggcgtccc tggaaaccat taaccgggtt ggcccctgcg aagcatacat
      661 ccaggttaat aaggtggagg atgtgcgagc cgttaaaagg agaaaagtag tggaccgggc
      721 caaagaactg tacaaaaata tgatggaaga agtgactcct gaaagcctca agatgattga
      781 ggaagttaag gaggccatgc gcatacatga aatcacagaa tttggaaacg aaaaacttcc
      841 agcagggcct aatgttgtct catcagatgc aatactagtt gttgaaggcc gggccgatgt
      901 tttaaacctt ctacgttacg gagtaaaaaa tgcggtggct gtggagggag taagcgttcc
      961 taaatcagtg gcagagctca ctaagaaaaa gacggttact gcctttttag atggtgatcg
     1021 tggcggtgac ttgatactta aggaactttt acaagtgggg gaactggact acgttacccg
     1081 ggctcccagg ggttctgaag tagaagacct gactaaagat gaggttatgg tggcattgcg
     1141 cgataagatt ccagtggagc agatctatca tgatttgggg gtaaaaactg agaaggtgga
     1201 gaaaaaaaca ccagacaaaa taggactact taaagggata ttgggtgatc tggaaggatc
     1261 tgggaatggt gaagtattgg atgattcttt aaacattatt aaagaggtta aagttgaaaa
     1321 tctctaccaa gaaattaata atctgcaaaa tggggcttat gctgtggtat ttgatggtgt
     1381 ggtcagtcag agactca
//
LOCUS       DAOQVW010000466         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_131440_length_1396_cov_2.110365, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000466 DAOQVW010000000
VERSION     DAOQVW010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131440_length_1396_cov_2.110365"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /gene="tuf"
                     /locus_tag="PKK85_08690"
     CDS             <1..1234
                     /gene="tuf"
                     /locus_tag="PKK85_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HNS26130.1"
                     /translation="AKEHMNLAFIGHVDHGKSTLVGHLLLQSGVIAEQQLSKGEDKFR
                     FVMDKLQEERERGVTIDLAHARFDTPKYEFTIVDCPGHRDFVKNMITGASQADAAVLV
                     VAIDDGVMPQTKEHAFLARTLGINQLIVAINKMDLVNYSEEKFNALKEDVGNLIKTVA
                     YKPDEIDFIPISAFEGDNITKPSENTPWYKGPSLVDSLNKLSAPEKPTNLPLRVPVQD
                     VYSITGVGTVPVGRVETGVMKKADNVIFEPPGVSGEVKSIEMHHEMLDKAEPGDNVGF
                     NVRGVGKNDIRRGDVAGHTDTPPSVAKEFTAQIVVLQHPGVITVGYTPVFHCHTAQVA
                     CTFLELQKKMNPATGAVEEENPDFLKTGNAAIVKVKPTKPMVIERIKEIPHMGRFAIR
                     DMGQTVAAGMCIDLVPAK"
     gene            1320..>1396
                     /gene="rps10p"
                     /locus_tag="PKK85_08695"
     CDS             1320..>1396
                     /gene="rps10p"
                     /locus_tag="PKK85_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266638.1"
                     /note="involved in binding tRNA to the ribosome; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNS26131.1"
                     /translation="MHKARIKLTGTDPEKLAYVCDQLKR"
BASE COUNT          469 a          320 c          318 g          289 t
ORIGIN      
        1 agcaaaagaa catatgaact tagcgtttat tggacacgta gaccatggta aatcaacact
       61 agtcggacac ctgctcctgc agtcaggggt aatagctgaa cagcagctat ccaagggaga
      121 agacaagttt aggtttgtta tggacaagct ccaagaagaa agagagagag gggttaccat
      181 cgaccttgcc cacgcaagat tcgacacacc caaatacgag ttcaccatcg tggactgtcc
      241 cggacaccgt gacttcgtga aaaacatgat caccggagca tcccaggcag acgccgcagt
      301 attagtggta gctatcgatg acggtgtaat gccccagaca aaagagcacg ctttcctggc
      361 aaggacactg ggaatcaacc agttaattgt agccatcaac aagatggacc tggttaacta
      421 cagtgaagaa aaattcaatg ccctcaagga agatgtggga aacctgatta agaccgtggc
      481 ctataagcca gatgaaatag acttcatacc tatatctgcc tttgaagggg acaacatcac
      541 caaaccatcc gaaaacaccc catggtacaa aggaccatca ctggttgact ctttaaacaa
      601 gttatccgca ccggaaaaac caactaacct accattacgt gtaccggtac aggacgtcta
      661 ctccatcaca ggtgtgggaa ccgtaccagt aggacgagta gaaaccggtg tcatgaaaaa
      721 agctgacaac gtcatattcg aaccaccagg tgtaagcggt gaagtaaaat ccatagaaat
      781 gcaccatgaa atgctagata aagcagaacc aggtgacaac gtaggattca acgtacgtgg
      841 tgttggtaaa aacgatataa gaagaggaga cgtagctgga catactgata caccaccatc
      901 agtggctaaa gaattcacag cccagatcgt ggtattacag caccctggtg ttatcaccgt
      961 aggttacaca ccagtattcc actgtcacac tgctcaggta gcctgtacat tcctggaact
     1021 ccagaagaaa atgaaccctg ctaccggtgc cgtggaagag gaaaatccag acttccttaa
     1081 gacaggtaac gccgctatag ttaaggtaaa accaaccaaa ccgatggtca tcgaaaggat
     1141 caaagaaatt ccacacatgg gaagattcgc tatccgtgat atgggtcaga ccgtggcagc
     1201 tggaatgtgt atcgatctgg tgccagcaaa ataaagatat ggtaaatatt accaagagga
     1261 aatggtggaa gggttcaaat tctacctgct tccttttatt ttttttatgg aggatctaaa
     1321 tgcacaaagc cagaataaaa ctaacaggca ccgacccaga gaaacttgcc tacgtatgcg
     1381 accagctcaa aagaat
//
LOCUS       DAOQVW010000467         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_131502_length_1395_cov_3.833582, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000467 DAOQVW010000000
VERSION     DAOQVW010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131502_length_1395_cov_3.833582"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1395
                     /locus_tag="PKK85_08700"
     CDS             <1..>1395
                     /locus_tag="PKK85_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNS26132.1"
                     /translation="TGDYIERGVDVPSIPKDKKWTFKPLVTSGAEVKGGDIIGEVQET
                     SAVTQKIMIPPNISGVLKNIASEGNYTVEEDIAEVDTNKGPVKIQMMQKWAVRVGRPY
                     KDKLDPDIPLITGQRAQDTFFPVAKGGTAAIPGPFGSGKTVTQQQLAKWADADIIVYV
                     GCGERGNEMTEVLKEFPELEDPKTGKPLMDRTVLIANTSNMPVAAREACVYTGITIAE
                     YFRDMGYDVALMADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAQFYERAGRVT
                     TVGTEDKTASVSVVGAVSPPGGDLSEPVTQNTLRICKVFWALDASLADKRHFPSIDWL
                     QSYSLYVDSVEGWWDGSVGEDWRKTRDEAMVLLQKESELQEIVQLVGPDALPDRERIT
                     LESTRMIREDFLQQNAYHEVDTYCSPSKQYQMLKTIIMFQEHATAALERGAASADLTA
                     LSVKEDVGRMKFIP"
BASE COUNT          387 a          327 c          377 g          304 t
ORIGIN      
        1 aaactggtga ctacattgag aggggagtgg atgttccatc catacccaag gacaagaaat
       61 ggaccttcaa accattagtc acctctggtg ccgaagttaa aggtggggac ataatagggg
      121 aagtgcagga aacctctgct gtgacccaga agataatgat ccccccgaat atatccggtg
      181 tcctgaaaaa catagcttct gaaggcaatt acactgtaga agaggatatt gccgaagttg
      241 ataccaataa agggcctgtt aaaattcaga tgatgcagaa atgggccgtt agggtgggaa
      301 ggccctacaa ggacaagtta gatcctgaca taccactcat aaccgggcag agagcccagg
      361 acaccttctt cccagtggct aaaggtggaa ccgccgctat acccggacca tttgggtctg
      421 gtaaaactgt aacccagcaa cagttggcta aatgggctga tgctgatatc atcgtctatg
      481 tgggttgtgg tgaaaggggt aacgagatga ctgaggttct gaaggaattt ccggagcttg
      541 aagacccgaa aactggtaaa ccactcatgg atcgtaccgt acttatagct aacacttcta
      601 acatgccagt ggcagccagg gaagcatgtg tgtacaccgg gatcaccatc gccgaatact
      661 tccgtgatat gggatacgac gtggccctaa tggccgactc cacctcccgc tgggccgagg
      721 ccatgaggga gatctcagga agattggaag agatgcctgg ggaagaagga taccctgcct
      781 atctggcatc tcgtcttgcc cagttctatg aacgagccgg ccgggttacc acggtaggta
      841 ctgaagataa aaccgcttcg gtcagtgttg tgggagctgt atccccgcca ggtggtgacc
      901 tgtctgaacc agtgactcag aacaccctcc gtatctgtaa ggtattctgg gcattggacg
      961 catcactggc agacaaaagg cactttccat ccatcgactg gttgcaaagc tactcccttt
     1021 acgtggacag tgtagaagga tggtgggatg gttccgtagg tgaagactgg agaaaaacca
     1081 gggatgaagc catggttctt ctgcagaagg aatctgaact ccaggaaatc gtacaactgg
     1141 tagggcctga tgctctccct gaccgggaaa ggattaccct ggaaagtacc cgtatgatcc
     1201 gggaggactt cctgcaacag aacgcctacc acgaagtaga tacctactgt tccccatcaa
     1261 aacagtacca gatgctcaaa accattatca tgttccagga acatgccact gcagctctgg
     1321 aaaggggggc agcatcggct gatcttaccg cattatccgt taaggaggat gttggaagga
     1381 tgaaatttat ccctg
//
LOCUS       DAOQVW010000468         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_131950_length_1392_cov_3.661930, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000468 DAOQVW010000000
VERSION     DAOQVW010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131950_length_1392_cov_3.661930"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKK85_08705"
     CDS             <1..529
                     /locus_tag="PKK85_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNS26133.1"
                     /translation="FVVILLVMVLSLAAVFMPVSFTLILILMLLNQIIYTLPPMRLKE
                     TPFAPLSSTATNSVLRLASCAVLLGNIFIVPPIVYIFMFMAGMATYLMYKSKSRDASI
                     VGVLCGLVLVYIIYSGEMNLIQFMVALVPSIAAGIPLYLSIFTSQERMLKLADILYHQ
                     VALGFFLICILIIIF"
     gene            complement(597..>1392)
                     /locus_tag="PKK85_08710"
     CDS             complement(597..>1392)
                     /locus_tag="PKK85_08710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNS26134.1"
                     /translation="DGDRVIVSAEHMDHDFEIQAKIIIAADGPESRVGRWAGLKTGTK
                     PKNMESCAQFEMVNVEMAEPDCIEFYFGSVAPGGYAWIFPKGDDIANVGLGILNTLTE
                     KTAYQHLLEFVDKNPATQNAQPVELNIGGDPVGGVIKDLVTDNVLVVGDAAGMVNPLT
                     GGGITSGMIGGRIAGEIAARAIKNESWSAKDLKDYKKRCQDDVGQSFKKYLKAKEYLL
                     NLSDEELDSIAEAFQKTDFDVINTSELIKKLIKVSPKALLKLGKIF"
BASE COUNT          358 a          316 c          256 g          462 t
ORIGIN      
        1 ctttgttgtt atcctactgg taatggttct cagtttagcc gcggtgttca tgccagtttc
       61 attcacacta attctgattc tgatgttgtt aaaccaaata atctacacac tgcctccgat
      121 gagattaaaa gaaacacctt ttgctccatt atctagtaca gccactaact ctgttttgag
      181 attggcttcc tgtgcagttt tgctgggtaa tatttttatc gtaccgccca ttgtttatat
      241 tttcatgttc atggctggga tggccacata tctcatgtac aagtctaaat cgagggatgc
      301 aagtattgtg ggagtgttat gtggattggt gttggtttat attatttatt ccggtgaaat
      361 gaatttaata caatttatgg tggcattggt tccatcaatt gccgccggaa tacccctata
      421 cctgtcaata ttcaccagtc aggaacgaat gctcaagttg gctgacattc tataccacca
      481 ggtagcctta ggtttcttcc ttatatgtat ccttattatc attttctaaa aaaggctaaa
      541 agtattttag gagtaggtta aaaataaccc taaaatatta taaacaacat caaatcctaa
      601 aatatctttc ctaattttaa aagagcctta ggagaaactt taatcaactt ttttatgagt
      661 tcactggtgt taattacgtc aaaatccgtc ttctgaaatg cttctgctat ggaatccaat
      721 tcttcatcgg acaagtttaa caaatattcc ttggctttaa gatacttctt aaaggattgg
      781 ccaacatcat cctggcagcg ttttttatag tccttcaaat ctttggccga ccatgattcg
      841 tttttgatag cccttgcagc tatttctccc gctattcgtc cgccaatcat gccactggta
      901 attccgccac cagtaagtgg gtttaccatc ccagctgcat ctcccaccac caaaacatta
      961 tctgttacca gatccttgat cactccaccc acaggatcac cgccaatgtt caattctact
     1021 ggctgagcat tctgggtggc ggggttttta tccacaaatt ctaagaggtg ttggtaagcg
     1081 gttttctcgg tgagagtatt caggatgcct aatcccacgt tggctatgtc atctcccttg
     1141 ggaaatatcc atgcataacc ccccggggct acactaccga aatagaattc gatacagtct
     1201 ggttctgcca tttctacgtt aaccatttcg aattgggcac acgattccat gtttttaggt
     1261 ttggtgccag ttttaagacc agcccaacgg cccactcttg actcggggcc atcggctgcg
     1321 attatgatct tagcttggat ctcaaaatcg tgatccatat gctctgccga gactattact
     1381 ctatctccat ca
//
LOCUS       DAOQVW010000469         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_133079_length_1385_cov_2.524060, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000469 DAOQVW010000000
VERSION     DAOQVW010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133079_length_1385_cov_2.524060"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKK85_08715"
     CDS             complement(<1..869)
                     /locus_tag="PKK85_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNS26135.1"
                     /translation="MKILEKSQLIKNQELSAKDNLESFCQIIKNVNPEINAFLEINKD
                     QAMEKAQDIDSRIENGEKVGKLAGLVIGVKNNISVEDFLITAASRTLENYQGSYDATV
                     VKRIKEEDGVIIGVTNLDEFAAGSSTETSYYGPTQNPAAPGLIPGGSSGGSAAAVAAG
                     MCDLALGSDTGGSIRNPASHCGIMGFKPTYGAVSRQGLLDLAMSMDQIGPLASDVSGI
                     ALLLDVIGGLDPRECTTLDWQAPLYTPALQDPLDHLNGVRLGVLKESFDVTDGPIVKI
                     IEDRLAELEGFGV"
     gene            complement(1006..1359)
                     /locus_tag="PKK85_08720"
     CDS             complement(1006..1359)
                     /locus_tag="PKK85_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNS26136.1"
                     /translation="MALATIFMIIGVYMGKLTSNGPWLLWLGLKPLAFHTVDYFPLLP
                     WLGVVSLGLFAGNTLYPNYQRRFRIPDISDHYLVKFASFLGRHSLLIYLIHQPILIII
                     LYLTGILQFSYLWLR"
BASE COUNT          406 a          312 c          280 g          387 t
ORIGIN      
        1 tccaccccga acccttctaa ttctgctagg cgatcttcta taattttgac tataggccca
       61 tccgttacat caaaggactc tttcagtaca cccagcctta caccattaag atgatctaat
      121 ggatcctgca gtgccggtgt atataggggt gcttgccaat ccagtgttgt gcactccctt
      181 gggtccagtc caccaataac atccaggaga agggctattc cactcacatc cgaagctaaa
      241 ggaccaatct gatccatgct catggccaga tccaaaagtc cctgcctgga gacagcaccg
      301 tatgtgggtt tgaatcccat aattccacag tgagatgcag ggtttcttat agaaccacct
      361 gtgtctgatc ctaaggctaa atcacacata ccagcagcca ccgcagcggc actgccacca
      421 ctggatccgc caggtataag tccaggggca gctgggttct gggtggggcc gtaataggat
      481 gtttcagtgg aactaccagc tgcgaattca tccaagttgg ttacgccaat gatgaccccg
      541 tcctcttcct tgatcctctt caccaccgtg gcatcgtaac taccctgata gttttccaga
      601 gtacgggaag cggcagttat aagaaaatcc tccacactta tgttattttt aactccaata
      661 acgagtccag ctagtttacc cactttttct ccattttcaa ttctagaatc aatatcctgg
      721 gctttttcca tggcttgatc tttatttatc tctaaaaagg cgtttatttc agggttaaca
      781 ttttttataa tttgacaaaa ggattccagg ttgtcttttg cacttaattc ctgatttttg
      841 atgagttgag atttttccag aattttcata aagccacctt gattaacgga agaaaaatgt
      901 tgtttaaaat cttagattat tgtttcatgg taggatatat aaaatgtatc cccatccccc
      961 agtggatgaa gcaaaataat agacaagctt cagaaaacat catgattatc gcaaccacag
     1021 ataactaaac tggagtattc ccgttaaata gagaattatt atgaggatgg gctggtgaat
     1081 caagtatatt aggagagaat gtctccctag gaaggatgcg aattttacca ggtaatgatc
     1141 agagatatct ggtattctaa accgccgctg gtaattgggg tacagggtgt tgcctgcaaa
     1201 aagtcccaat gaaaccacac caagccaagg tagcagagga aaatagtcaa cagtatggaa
     1261 tgccagaggt ttaaggccta accacagaag ccagggtcca ttggaggtca acttccccat
     1321 ataaacacct attatcatga aaatagtggc aagagccaaa ttaagatatt ttttatctaa
     1381 aaatg
//
LOCUS       DAOQVW010000470         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_133163_length_1385_cov_1.615789, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000470 DAOQVW010000000
VERSION     DAOQVW010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133163_length_1385_cov_1.615789"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKK85_08725"
     CDS             complement(<1..359)
                     /locus_tag="PKK85_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HNS26137.1"
                     /translation="MKFAWVMAEGMPWNKKKGFITTTLESGIDHIVDFTDTENIQKLG
                     NVKIISDVEDSDIFLVGRNGEGDGTIPLPKVLSESKDLSTVRRIKSKEEPVTAYVEIT
                     SKEHEQLAALLGKYADYL"
     gene            complement(423..1217)
                     /locus_tag="PKK85_08730"
     CDS             complement(423..1217)
                     /locus_tag="PKK85_08730"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HNS26138.1"
                     /translation="MIGKKIRIERILDRKTGRCVIVPMDHGVSVGPIDGIIDMAETID
                     EVASGGANAVIVHKGIVGSGHRGYGRDIGLIIHLSASTALGPDPDNKVLVTSVEKALQ
                     MGADAVSVHVNVGSKKEPEMLNILGTTAEICDDWGMPLIAMMYPRGEKIKDEHHADVV
                     KLASRAGAELGADIIKTNYTGDPETFREVIEGCPVPLVIAGGPRVETDRELLEMVKNS
                     VDVGGAGVAIGRNIFQAKSPRKTTRAIAEIVHNDMEVDDALKILNG"
BASE COUNT          283 a          347 c          288 g          467 t
ORIGIN      
        1 aggtagtcgg catatttacc cagtaaagcg gccagttgtt catgttcttt actggtgatc
       61 tccacatagg cggtgactgg ttcttcttta cttttaattc ttctaaccgt agataaatcc
      121 ttggactcag ataaaacttt tggtaaaggt atggttccgt ctccttcacc atttctaccc
      181 actaggaaga tatctgaatc ttccacatcc gagattatct ttacattgcc cagtttttgg
      241 atgttttctg tgtcggtgaa gtccacgata tggtctattc ctgattccag ggtggtggtt
      301 atgaatccct ttttcttatt ccatggcatg ccttcggcca tgacccatgc gaatttcatc
      361 ttactatacc tcctagtgtt tattcatcgt gtaagctcta aattcatttg gtaagcttta
      421 aattaaccgt tgagtatctt cagggcgtca tctacttcca tgtcgttgtg cacgatttca
      481 gcgatggctc tggttgtctt tcggggtgat ttggcctgga aaatattccg gccaatggcc
      541 acacctgcac caccaacatc taccgagttt ttaaccatct ccagtagttc acggtcggtt
      601 tcaacccggg ggccgccggc tattaccagt gggactggac atccctctat aacttcccgg
      661 aaagtctctg ggtctccggt gtaattggtt tttatgatgt ctgcccctaa ctctgccccg
      721 gcccgggagg ctagtttaac cacgtcggcg tggtgttcgt ccttaatttt ttctccccgg
      781 gggtacatca tggcgatgag tggcattccc cagtcgtcgc atatctcggc ggtggtaccc
      841 agtatgttga gcatctctgg ctccttcttc gatcccacgt tcacgtggac ggaaactgca
      901 tcggcaccca tttgtaatgc cttctccacg gatgttacca gtaccttgtt gtccgggtct
      961 ggtcccagtg ctgtgctggc tgataagtgt attatcagtc cgatgtccct tccgtatcct
     1021 ctatgcccgc ttccaactat tcctttatgt actataacgg cgtttgctcc tccgctggcc
     1081 acttcgtcga tggtttctgc catgtcgatg atcccatcta tggggcccac ggagacaccg
     1141 tgatccatag gtacaattac gcatcgacct gtttttcggt cgagtatcct ttctattcta
     1201 atttttttac ctatcataag accctcctcc catgagtatg aattcaatta atagaaaatg
     1261 ttacaattga tactgtatga ggatttacat tagctgatat aaaaaactaa tccctatttt
     1321 tatgggtact ctttcctttc cctgatttcc attatggtct ttgcttttaa ttttcaaatg
     1381 gggtt
//
LOCUS       DAOQVW010000471         1383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_133399_length_1383_cov_2.664157, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000471 DAOQVW010000000
VERSION     DAOQVW010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133399_length_1383_cov_2.664157"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1024
                     /locus_tag="PKK85_08735"
     CDS             179..1024
                     /locus_tag="PKK85_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS26139.1"
                     /translation="MNVIETKDISYSYPDGTLALKDVEFNAAKGKIIALLGPNGAGKS
                     TLFLHFNGILKPNKGTVRVNGEPLKYDKAGLMKVRQKVGIVFQNPDDQLFAPTVMEDV
                     AFGPMNIGLPDEEVEERVKESLERVGMKGMENKAPHHLSGGQKKRVAIAGILAMRPEI
                     MVLDEPTSGLDPKGASQIMHLLYNLNQEGMTIVISTHDVDLVPLYAYNVYIISEGKII
                     KEGSPLEVFEDVETIRGANLRLPRIAHLMEILGKEDKLPFDKPYPLTIGEARRRILKE
                     FDVKG"
     gene            complement(1050..>1383)
                     /locus_tag="PKK85_08740"
     CDS             complement(1050..>1383)
                     /locus_tag="PKK85_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS26140.1"
                     /translation="NLVKNYDITFNILRADITPKGGKMLIEISGTQQKQAVEFMEKEG
                     IHLNPINKVVKKDEEKCIDCGACISLCPVEAIKPAEDWTVEVDDLKCIGCSFCTNSCP
                     TRAIRVME"
BASE COUNT          417 a          300 c          304 g          362 t
ORIGIN      
        1 taagtggggc tatcataatt ataataggat tgattacatt gttacatccc ataattgaat
       61 atataaccaa cctacatcta taagatatca gcattcaaaa atattgtcca taaattaaat
      121 tcgttagatt caatttcaaa aaaaatttaa tctcaaatta tccatcaaag gtgaatcaat
      181 gaatgtaata gaaactaaag atatcagtta cagttatcca gacggtactc tcgccctgaa
      241 agatgtggaa ttcaacgcag ctaagggtaa aataatcgcc cttctaggac ctaacggggc
      301 gggtaaatcc acactatttc tacatttcaa tggaatttta aaacccaata aaggaacagt
      361 ccgggtaaac ggagagccgc tcaagtatga taaggcagga ctgatgaagg tccgccagaa
      421 ggtgggcata gtattccaga accctgatga tcagttgttt gcacccaccg taatggagga
      481 cgtggccttc ggacccatga acattggttt acctgatgaa gaggttgaag agagggttaa
      541 agaatccctg gaaagagtcg gtatgaaggg aatggagaac aaagcacccc accacctgag
      601 cgggggtcaa aaaaagcgag tggccatcgc cggtatcctg gccatgcgtc cggagataat
      661 ggtcctagac gagcctacat ctggtctgga tcctaagggc gcatcgcaga tcatgcatct
      721 actctataat ctgaaccagg aaggtatgac catcgtcatc tccacccatg atgtcgacct
      781 ggtaccctta tatgcctata acgtgtacat cattagtgaa gggaaaatca tcaaagaagg
      841 aagtcccctg gaggtcttcg aggatgtgga aaccatacgt ggggctaacc taagactacc
      901 ccgtatagcc cacctcatgg agatactggg aaaggaggat aaactaccct tcgataaacc
      961 ctatccccta accatcggtg aagcgaggag aaggatcctc aaggaattcg atgtgaaagg
     1021 gtaaaatgaa ctctcacttt attgaagaac tactccataa cccttatagc cctggtggga
     1081 caggaattgg tgcagaagct gcagccaatg cacttcaggt catctacttc tactgtccag
     1141 tcttcagctg gtttaattgc ttccaccggg catagtgaaa tacacgcacc acaatctata
     1201 catttttctt cgtctttttt aaccacttta tttatggggt tgaggtggat tccctctttc
     1261 tccatgaatt ctacggcctg tttctgctgg gttccgctga tttcaatgag cattttacct
     1321 cctttggggg ttatatctgc ccgtaggatg ttaaaagtga tatcgtagtt tttcaccaga
     1381 ttg
//
LOCUS       DAOQVW010000472         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_133574_length_1382_cov_2.395629, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000472 DAOQVW010000000
VERSION     DAOQVW010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133574_length_1382_cov_2.395629"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKK85_08745"
     CDS             complement(<1..491)
                     /locus_tag="PKK85_08745"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HNS26141.1"
                     /translation="MIVEEHIQNLLKKGKIHLTLLDPDEQTPDEALIKAEEAVAAGSH
                     GIMLGGSITNAQDLDATAKMIRENLDTPIILFPGNITGVSSYAHAIFFMSVLNSTNPY
                     WIIGAQALSASLIKRIGIQTIPMGYIIVEPGGTAGWVGDAKPIPRNKPDIAAAYALAA
                     EFLG"
     gene            complement(566..712)
                     /locus_tag="PKK85_08750"
     CDS             complement(566..712)
                     /locus_tag="PKK85_08750"
                     /inference="COORDINATES: protein motif:HMM:NF003161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HNS26142.1"
                     /translation="MARFEEAENRLFKIKICLKCNARNPPTAKTCRKCGHKGLRFKAK
                     EPRG"
     gene            complement(716..1225)
                     /locus_tag="PKK85_08755"
     CDS             complement(716..1225)
                     /locus_tag="PKK85_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HNS26143.1"
                     /translation="MKVTVYHAGQCNPKRCTTRRLDKKSYIKMVTHLNQLPKGALVLD
                     PFASKAVSKEDRETVLKHGIVGLDCSWRKIDKFSPIFRGKKNHRSLPFLVAANPTNYG
                     KPCILSTAEAVAATFYILGLKNNAIQIMSPFKWGPHFLELNHELLEAYSLAKSSKEVV
                     KIQNEFIGD"
BASE COUNT          370 a          286 c          284 g          442 t
ORIGIN      
        1 cccagaaact ccgcagccag ggcataggcc gcggcgatat caggtttgtt acggggaatg
       61 ggtttggcat ctcccaccca tcctgctgtg cctccaggtt ctacgattat gtaacccata
      121 ggaatggtct gtatccctat tcttttgatt aaagaggcac tgagggcctg ggcaccgatg
      181 atccagtaag gattggtgga gttcaaaaca ctcataaaga aaatggcatg ggcataactg
      241 ctcacaccag tgatgttacc tggaaaaagt ataatgggag tatctaggtt ctccctgatc
      301 atcttggccg ttgcatctag atcctgtgca ttggtgatgg aacctcccag catgatacca
      361 tggctaccag cggccacagc ttcttcagct tttatcaaag cctcatctgg agtttgttca
      421 tctggatcta gtaaggtcag atgtattttg ccctttttaa gtagattttg gatatgttct
      481 tcaactatca tgttctatct caccaagata taacatcacc aaaacggaaa ttaaaataag
      541 atcaggtcca ttgaccagga ctggtttaac cccttggttc tttggctttg aatctcaaac
      601 ccttgtgtcc gcatttacga caagttttag ccgttggcgg atttcgagcg ttacatttta
      661 aacatatttt gatcttgaat agtctgttct ctgcttcttc aaatctagcc atcaattagt
      721 ctcctataaa ttcattctga atctttacaa cttctttact tgatttagcc agggaatagg
      781 cctctaaaag ctcatgattg agctctagaa aatgaggtcc ccacttgaat ggggacatta
      841 tctggatagc attatttttc agtcccagta tataaaaggt tgcggccact gcctcagctg
      901 tggacagtat gcagggtttg ccatagttgg tggggttggc agccaccagg aatggaaggg
      961 atcggtgatt ctttttaccg cgaaatatgg gtgaaaattt atcaattttc ctccacgaac
     1021 aatctaaacc aacaatacca tgttttaaaa ctgtttccct gtcttctttg gaaacagcct
     1081 tagatgcaaa ggggtctaat accagagcgc ctttaggaag ttggttaagg tgagtgacca
     1141 tttttatgta gcttttctta tctaggcgcc gtgtggtgca acgtttgggg ttgcactgtc
     1201 cagcgtgata cacagttact ttcatgatat attactaaaa tatccttgtt accacaacaa
     1261 ttgattttag aaaaaattga atgccaatac tattgttttt atgtacttca cttattaaat
     1321 ttgtccaaaa tgtacggtta cttagattta tattatcatc taacatttca tatatatatc
     1381 gg
//
LOCUS       DAOQVW010000473         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_133727_length_1381_cov_2.426848, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000473 DAOQVW010000000
VERSION     DAOQVW010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133727_length_1381_cov_2.426848"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..>1381
                     /locus_tag="PKK85_08760"
     CDS             97..>1381
                     /locus_tag="PKK85_08760"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HNS26144.1"
                     /translation="MEYILKNGIVYDPANEIAGEKMDVMFKDGKIVSSVSSDAKVLDV
                     TDKIVMPAGVDPHAHVAGPKLVVGRLYRPEDSRKGVTPKTDVCRSESGFSIPSCPATG
                     YRYSRMGYGTVTEAAMPPLEAKHTHEVITAIPNIDVTPLPLFGNNWFVLQWAKEGKVE
                     EIAAFVSSWLKIVKGYGIKIVNPCGTEAWGWGGNVHGIDDPVPYFDVTAREVIRALAQ
                     ANEMLGLPHSIHVHPNDLGHPGNYPTTVNTLDCIKDIEKSSGLRDQTIHLTHAQFHSY
                     AGTSWRDVESGAKEVADYINNNKHVTCDIGQVTLDETTTMTADGPMEFDLHSLNGLKW
                     ANKDIELETGSGIVPFIYSGKAPVPSFQWGVGLELFMRIKDPWQVCLTTDHPNAGPFI
                     RYPRIISWLMSAERRQEMMDNGEVRVWSYKRTGLGS"
BASE COUNT          428 a          312 c          331 g          310 t
ORIGIN      
        1 tgccgagacg acgaatggat cattaaagag ctcctagaaa gagttcagaa gatcaaagat
       61 caagaagccg cagtagccag taaataaggt gatttcatgg aatacatact aaaaaacggt
      121 atagtttacg acccggccaa cgaaatcgcc ggtgagaaga tggacgtcat gttcaaggat
      181 ggaaaaatcg ttagtagcgt ctcttctgat gcaaaagtcc tggatgtaac agataagatt
      241 gtaatgcctg ccggtgtgga tccccacgcc cacgtggctg gaccaaaact cgtggtaggg
      301 cggttataca ggcctgaaga ctcaagaaaa ggtgtaactc ctaaaacaga tgtttgcaga
      361 tcagaatctg gattttccat acccagttgc cctgcaacag gatacagata ctctaggatg
      421 ggttatggaa cagtgactga agctgccatg ccccctctag aagcaaaaca cacccatgaa
      481 gtgatcactg ccatccccaa catagatgtc actccacttc ctttgtttgg taacaactgg
      541 tttgtgttac agtgggctaa ggagggaaaa gtagaggaaa tagccgcatt cgtgtcctca
      601 tggctcaaaa tagttaaagg ttatggaata aaaatcgtta acccctgtgg aactgaagca
      661 tggggatggg gaggaaacgt tcatggaatt gatgatcctg taccctactt tgatgtaacg
      721 gctagagaag tgatcagggc tctagcacag gccaatgaaa tgttaggact accacactcc
      781 atacacgtac atcccaacga cctggggcac ccaggaaact atcctaccac ggttaacacc
      841 ctggactgca ttaaagacat cgaaaaaagc agcggtttac gagaccagac catacaccta
      901 acccatgccc agttccattc ctatgctgga accagctggc gggatgtgga atcaggtgcc
      961 aaagaagttg cagattacat aaacaataac aaacatgtaa cctgtgatat cggtcaggtg
     1021 accctggatg aaaccactac catgactgcg gatggtccaa tggaatttga cctgcactct
     1081 ttgaatggtc ttaaatgggc taacaaagac atcgaactgg aaactggatc cggaattgtt
     1141 ccatttattt actctggaaa agcaccagtt ccatctttcc agtggggtgt aggactagaa
     1201 ctgttcatga gaataaaaga tccctggcaa gtatgtttga ccactgacca ccccaatgct
     1261 ggtccattca tccgctaccc cagaatcatc tcttggctca tgagcgcaga gcgccgacaa
     1321 gaaatgatgg ataacgggga agtacgggta tggtcataca aacgaactgg cctgggaagc
     1381 t
//
LOCUS       DAOQVW010000474         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_133855_length_1380_cov_2.842264, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000474 DAOQVW010000000
VERSION     DAOQVW010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133855_length_1380_cov_2.842264"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1380
                     /locus_tag="PKK85_08765"
     CDS             <1..>1380
                     /locus_tag="PKK85_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNS26145.1"
                     /translation="WLVFLNIIVGIFIAFVFFGKRTRKMGHNLNALTFPEFLSRRFNS
                     RFIQYFSGAVIFIGMPLYASVVLIGMARFVETTLSIDYNIALVVMAIIVAAYVIFGGI
                     RGVMYTDALQGTIMFLGMLFLLGSIYYLLGGVTNAHQALTNLVNVVPASATAKATATG
                     FTGWTSMPSLGSPFWWTLVSSLILGVGIGVLSQPQLVVRFMTVKSNRELNRAVLMGGV
                     FIFVTTFAAYVVGSLSNVYFFQLTGNTAVQAAGGNLDKVIPTFISAAMPLWFAYLFMI
                     TLLSAAMSTLSAQVHVQGTALGHDIYETLRGDKKKGSSVLIARVGILVAVVIAVILGF
                     ILPTSIIAVGTSMWFGITAAAFLSMYVAALFWKRATKAGAIAGLVGGSLMSLFWLLFG
                     FKKSAEAVGICQALTGKVVIINTLPWLTIDPIVISLPLAALLTVLVSLMTKPPEKEHM
                     DKCFAGLDT"
BASE COUNT          317 a          358 c          348 g          357 t
ORIGIN      
        1 ctggctggta tttttaaaca tcatagtggg aatcttcata gctttcgtct tttttgggaa
       61 aagaacaagg aaaatgggac acaacctcaa cgctctcacc ttccccgaat ttctatcccg
      121 tcgttttaac agccgattca tccagtactt ctctggggcg gtgatcttca ttggaatgcc
      181 cctctacgca tcggtggttc tgattggtat ggccaggttc gtggaaacca ccctcagcat
      241 agattataac atagccctgg tggtaatggc tattatagtg gcggcttacg tcatattcgg
      301 gggtatacgt ggggtgatgt acacagatgc cctgcagggg accatcatgt ttctggggat
      361 gctcttcctc ctgggatcaa tctactacct cctgggcgga gttaccaatg cccatcaagc
      421 cctcaccaac ctggtgaatg tggtgccagc cagcgccacg gccaaggcca ctgcaactgg
      481 attcacaggc tggacatcca tgccctcact gggaagtccc ttctggtgga ctctggtatc
      541 cagtctaata ctgggggtgg ggattggggt gctgtcccag ccccaactgg tagtccgttt
      601 catgaccgtc aaatccaacc gggaactcaa tcgggcagtt ttaatgggtg gggtgttcat
      661 tttcgtcacc accttcgcgg cttacgtggt ggggtccttg tccaacgtgt acttcttcca
      721 attaactggt aatacagctg ttcaggcagc aggaggcaac ctggataagg tgatacctac
      781 cttcatatcc gcggccatgc ccctgtggtt cgcctatctg ttcatgatca ccctactatc
      841 cgcggccatg tccaccctat cagcccaggt acacgtgcag ggaacagccc taggtcatga
      901 tatctatgaa accctgagag gagataagaa aaaaggctcc tccgtcctaa tagcccgggt
      961 ggggatccta gtggcggtgg ttatagctgt gattctggga ttcatactac caaccagcat
     1021 catagcggtg gggacgtcca tgtggtttgg aataactgct gctgcttttt tatcaatgta
     1081 tgtggcagcc ctcttctgga aacgggccac caaagccggg gccatcgcag gattggtggg
     1141 agggagttta atgagcctat tctggctact ttttggtttc aaaaaatcag ccgaagcagt
     1201 gggaatatgc caggccctca ccggaaaagt ggtcatcatc aacaccctac cctggctcac
     1261 catagacccc atagtcatat ctttacctct agcagcattg ttaacagtgt tagtaagcct
     1321 catgaccaaa ccacccgaga aagaacacat ggacaagtgc tttgcaggtt tggatacctt
//
LOCUS       DAOQVW010000475         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_134863_length_1374_cov_2.890068, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000475 DAOQVW010000000
VERSION     DAOQVW010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134863_length_1374_cov_2.890068"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..>1374
                     /locus_tag="PKK85_08770"
     CDS             154..>1374
                     /locus_tag="PKK85_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26146.1"
                     /translation="MDKTNLLQVLDTIKNKKDEGLSTIDGRNLKKKAMANYVEIVDEL
                     LKLLDSEIHDFQNKMDSQTLKTVEKFLEEISTVFEKIDEKNQVKLGTFHKYLNEYEKT
                     LKKFLETLKKFEDAKIELLVKAEATRRTEDDIIRHIAELEFEGSDEEVTHIKDLTENL
                     VKEIEKSREKEIKEDIKIDKPVSLVEGNYKLTPSEVRDWLEKIPEDDYFLVGINPEIA
                     KPEWMVLTVLPVPPVTVRPSITLETGERSEDDLTHKLVDILRINQRLKENMEAGAPQL
                     IVEDLWELLQYHVTTYFDNEASGVPPARHRSGRPLKTLAQRLKGKEGRFRSNLSGKRV
                     NFSARTVISPDPNISINEVGVPEMIAIEVTVPVYVTEWNIEQMKKHIENGSKIHPGAN
                     YVIRPDERKIRVYDE"
BASE COUNT          489 a          242 c          303 g          340 t
ORIGIN      
        1 ccttgtaaaa gaaattgagg gttccggtct gaaagtagat tcaaaaatcg tagaacaaat
       61 agaaaaaagc tttgaaactc tccaaccgtt ctagaagaac aattaacttc cagtctccgg
      121 atacgggaac ttgaatccca atatgatata tcaatggata aaacaaatct tctacaagtt
      181 ctcgatacta taaaaaacaa aaaagatgaa ggtctatcca ctattgatgg gcgtaatctt
      241 aaaaagaagg caatggccaa ttatgtggaa atagttgacg aactcttaaa attacttgat
      301 agcgaaatcc atgacttcca aaacaaaatg gattcccaga ccttgaaaac agttgaaaaa
      361 tttttagagg aaatcagcac tgtttttgag aaaattgatg agaaaaacca agtaaaattg
      421 ggaacattcc ataaatatct aaatgaatat gaaaaaacat taaaaaaatt cttagaaacc
      481 ttaaaaaaat ttgaagatgc aaaaatcgag ttgcttgtca aggccgaagc cactcgccga
      541 actgaagatg atatcatcag acatatagct gaacttgaat ttgaaggttc ggatgaagaa
      601 gtcacccata taaaagattt aactgagaat ttagtcaaag aaatcgaaaa atcacgggaa
      661 aaagaaataa aagaagatat aaaaatagac aaacccgtgt ctctggtgga gggtaactac
      721 aagctcacac ctagtgaggt gcgggattgg cttgagaaaa tcccggaaga tgattatttc
      781 ctggtgggaa tcaaccctga aattgcaaaa ccagaatgga tggtcttaac tgttttacct
      841 gtcccaccgg taacggttag gccgtcaatt accctggaaa caggtgaaag atcagaggat
      901 gatttaaccc ataagttggt ggatattctg cgtataaatc agcgtcttaa ggagaacatg
      961 gaggctggag cgccacaact cattgtggag gatttgtggg aacttctgca gtatcatgtt
     1021 accacttact ttgacaacga ggcttctgga gttccaccag cccggcatag atcaggaagg
     1081 ccacttaaaa ctttggcaca acggttaaag ggtaaagagg ggcgtttcag aagcaacctc
     1141 tcgggtaagc gtgttaactt ctcggctcgg actgttattt cccctgatcc taacattagc
     1201 attaatgagg tgggtgtgcc ggagatgata gccattgagg tcacggtacc ggtttacgtt
     1261 acagagtgga acattgaaca gatgaaaaag cacattgaga atggttccaa gatacatccc
     1321 ggtgctaatt acgttatccg gcctgatgag cggaagataa gggtttatga tgag
//
LOCUS       DAOQVW010000476         1371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_135323_length_1371_cov_3.490881, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000476 DAOQVW010000000
VERSION     DAOQVW010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135323_length_1371_cov_3.490881"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PKK85_08775"
     CDS             complement(<1..641)
                     /locus_tag="PKK85_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HNS26147.1"
                     /translation="MGIKATPRFLMSLFLVMILILNLGSVSAAQWEVGVGQTYENIQL
                     AIDDAKTIDGDVINVHSGIYSEDVRLNKKLTLQANTGDDVRLQPVNTGFTVVSEGSGS
                     TIDGFSIDNSPQGTGINISADNCTLKNNQINGGTTGIAVSGANTSLTNNVISGQSSTG
                     ILGNLTGGFFTVTGNIISNITGTGTLAGITVSTNGSLSLINIKGNTISHFNAM"
BASE COUNT          404 a          259 c          240 g          468 t
ORIGIN      
        1 tccattgcgt tgaaatggga tatggtgttc cccttaatat taatgagcga taaactgccg
       61 ttggtactaa cagtgatacc tgctaatgtt ccagtacctg tgatattgga tattatgttt
      121 ccagtgacgg tgaaaaagcc tccagtgaga tttccgagaa tacccgtact tgattgtccg
      181 gatatgacat tgttagttag gctagtgttg gcaccggaaa ctgcgattcc agtagtacca
      241 ccgttgatct ggttattttt caaggtacag ttatcggcgc tgatattaat accggttccc
      301 tgaggactgt tatcgatgct aaaaccatct atagtactcc cactaccttc agatactact
      361 gtaaaaccag tgtttacagg ttgtaatcgg acatcatctc cagtattggc ttgtagggtt
      421 aattttttat ttaatctcac atcttcagaa tagattccgc tgtgaacgtt gataacatca
      481 ccatcaatgg tttttgcatc atcaattgcc agttgaatat tttcataggt ctgacccact
      541 ccaacttccc actgtgctgc tgatacacta ccaaggttta aaattaaaat catgaccaga
      601 aataaagaca ttaaaaatct cggtgtagct tttattccca tattatccac ccaataataa
      661 atcaacatgt aaaaagtaag aattctttta ttaacaatat tattagaacc tttccatttt
      721 gatttaaaag tactagatta aagattatct aaaaaaaatc ggggtgttgt agatattggc
      781 agttttgaga agatagcaaa gatttgatca tgaatttatt tctcgtgttt gggcctagaa
      841 caatagtgaa cgaaattgtt agtgggacgt gtggtagtga tgggcagttt taatcctagt
      901 ggcgtgtatt cgtccattta gaacacctcc ctccacctaa gctacatata tgaacaaata
      961 attataaaat gcaatttttt cttttaacat ccttcattct ggagctagat caagaaaaaa
     1021 aatctaataa tctcaatttt tatccaaaga taaactattc aactggtttt ggtggcgagg
     1081 attctcaact tcattatcca ctgtaagaag tttttcccct taccataagt cgtaatatac
     1141 cggaaatacc atccagactg caccatatga taatatgggt tataaatcac atgagtttca
     1201 gcttttaccc tgtgttctga gtactgtttc ccgaactggt aacccctgcc tgttcttgtg
     1261 atctttttat tcagattacc tttgatcagg ccatttttat agttcataat agtgacattt
     1321 tttatggttc tttgaccctg gataattctt taaattgtgg gtcctcctaa t
//
LOCUS       DAOQVW010000477         1371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_135325_length_1371_cov_3.330547, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000477 DAOQVW010000000
VERSION     DAOQVW010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135325_length_1371_cov_3.330547"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PKK85_08780"
     CDS             <1..1033
                     /locus_tag="PKK85_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNS26148.1"
                     /translation="FSDHYLEVPYDLSDVFFIATANSLRDIPGPLRDRLEIIEIGSYT
                     SHEKFHIGKNHLIDLVLKDNGLSRDQLQITDEALQTIIEKYTREAGVRNLKRQLATVA
                     RVASEKIVLGKVDLPYIIKEDMLYDMLGHQITQINEVGKKNPPGVVTGLAWTPVGGDI
                     LFIEGAFMPGTGKLTLTGQLGDVMKESAKISQSLIRSRLAFHLKNMEFDKKDLHIHVP
                     SGAIPKDGPSAGMALLTTIASLVTGQAVDSKLAMTGEISLRGAVLPVGGIKEKVLAAH
                     RAGIKRVILPLENKNDLDDIPEDVKGELEFILVETVEDVIRETIGIELPKPVLMGVSS
                     DTLAGGAGA"
BASE COUNT          391 a          344 c          335 g          301 t
ORIGIN      
        1 attctctgat cattacctgg aggtacccta cgacctgtct gatgtcttct tcatagccac
       61 cgccaactcc ctgagggaca tacccggacc cctgcgggat cgtctggaaa taatcgaaat
      121 cggcagctac accagccacg agaagttcca cataggaaag aaccatctca tcgacctggt
      181 gctgaaagat aacggcctca gcagggatca gctgcagata accgacgaag ccctgcagac
      241 catcatcgaa aagtacaccc gggaagcagg ggttaggaac ctcaaacggc aacttgccac
      301 ggtggccagg gtggcgtcgg agaagatagt gctgggtaag gttgatttac cctacataat
      361 aaaagaggac atgctgtacg acatgctggg acaccagata acccagatca atgaggtggg
      421 taagaaaaat cctcccggag tggtgactgg cctggcctgg actccagtgg gaggggatat
      481 cctcttcatc gaaggtgcct tcatgcctgg aaccggcaaa cttaccctga ccggtcaact
      541 gggggatgtg atgaaggaat ctgctaagat ctcccagagc ctcattcgct ccagactggc
      601 cttccacctg aagaacatgg aattcgataa aaaggatctg cacatccatg tcccatccgg
      661 ggccataccc aaggatggac cctctgcagg gatggcacta ttaaccacca tagcatccct
      721 ggtcactggt caagccgtgg attctaaact ggccatgact ggtgaaatct ctctacgagg
      781 cgctgtactg cccgtggggg ggataaagga gaaagtcctg gccgcccacc gggccgggat
      841 aaaacgggtc atcctacccc tggagaacaa aaacgacctg gatgatatcc ctgaagatgt
      901 gaagggggaa ctggaattca tcctggttga aactgtggaa gatgtcataa gggagaccat
      961 cggtattgaa ctgcccaaac cagtgctgat gggtgtcagc tccgacactt tagccggtgg
     1021 agccggagcc tagtcccaca atgcccaaga aaaattctat tccatctcca tcccagggga
     1081 tggaaacttt tttttccaga attaaatcat ggaaacgcat atcattcccg gtgaaaaggt
     1141 tggagatttt cttttacaaa tcctggtcca agtatggact ccagtcacca atccctagga
     1201 cctgtacatt ttttttgata gtttacccat aaaaagaaaa accaactcct attttttctg
     1261 gatcaaaagg ttattccttc atggttaaac agacggtaca tcaagacttt gatgaagtcg
     1321 gttattacaa aggcgataac agcataaatc catacaaaaa tggccaaata c
//
LOCUS       DAOQVW010000478         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_136490_length_1364_cov_3.681436, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000478 DAOQVW010000000
VERSION     DAOQVW010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136490_length_1364_cov_3.681436"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1211
                     /locus_tag="PKK85_08785"
     CDS             192..1211
                     /locus_tag="PKK85_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNS26149.1"
                     /translation="MNMDHNLDQDRIEQLIHGIRRDIGHKDLEPDIQEVVWNRNIQEL
                     VIITRDRPGKSTVIGKGGWVAGRLKEELGVKSVHVEAYTDLLVRRHRMELAQHRLDQI
                     RSHHHFPALNNLSHLLEKRIERPYYLKTLLGDVEKPDASSLSVVALSGGVDSSFSLIL
                     SHILGFQIQAVTVDPGSVILPGYFKQNVEHLTQKLQVPHEYLTLDLSEVIQESLEGRY
                     HPCGRCSKLIQEKVLKQAQRLGAQFIFYGDLLSTGANSLHWMDDILGINLPALLSATK
                     GEVKGITHSWGVDRQAGYGCPLLKEVHKKHPSMRRYSIQRIERETRAGVLEPGEAMDM
                     IMRLI"
BASE COUNT          387 a          337 c          336 g          304 t
ORIGIN      
        1 ctgtatagta gatctggaga ttttgccagt tacctccact cccagagggt ggattactac
       61 gtggacagcc tcagcaaccc caaaccagac ctgccaggct accggcaggt ggccagcttc
      121 cagatggtaa ccatctaccg cagaacatga atacttatac ttagcttatc agaacaattc
      181 aaggaaataa tatgaacatg gatcacaacc tagaccagga tcgcatcgaa caactaatcc
      241 acggcatcag acgagacatt ggccacaagg atcttgagcc agatatccag gaagtggtgt
      301 ggaataggaa catccaggag ctggtgataa taaccaggga caggccaggt aagtccactg
      361 tcattgggaa gggaggctgg gtggctggcc gccttaaaga agaattagga gttaaaagtg
      421 tccatgtaga ggcttacact gatcttttag tacgccggca ccgtatggaa ctggcccagc
      481 accggctgga tcagataaga tcccaccacc acttcccagc ccttaacaac ctatcccatc
      541 tactggagaa gaggatagaa agaccttact acctgaaaac cctcctagga gatgtggaga
      601 aacccgatgc atcttcactg tcggtggtgg ccctatccgg gggtgtggac agcagcttct
      661 ccctaatcct cagccatatc ctggggttcc agatccaggc ggttaccgtg gatccaggga
      721 gcgtaatcct ccctggatac tttaaacaga atgtggagca cctcacccag aaacttcagg
      781 tgccccacga gtatctaacc ctagacttga gtgaggtgat acaggagtcc ctggagggac
      841 gttatcatcc ctgcgggcgg tgctccaagt taatccaaga aaaagtttta aaacaggccc
      901 agagattagg ggcccagttc atattctacg gggacttact atcaactggg gctaactctc
      961 ttcactggat ggatgatatt ctaggaataa acctccccgc cctattatca gccactaaag
     1021 gtgaggttaa aggaataacc catagttggg gggtggatag gcaagctggt tatggctgtc
     1081 cattgctgaa ggaagtgcac aaaaaacatc cctccatgcg ccgctactcc atccagcgca
     1141 ttgaaagaga aacccgggct ggagttctag agccggggga ggccatggat atgatcatgc
     1201 gacttattta attaattcta ctataccgta ggccaggagg aacactccca ccaagtaacc
     1261 caccagctgg ggatatatga aaaggaggat cccgattata atggcggcga tgcctattat
     1321 ctgacgaata ttttctttct ccatgcctat atttttgtaa aaag
//
LOCUS       DAOQVW010000479         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_136695_length_1363_cov_2.428899, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000479 DAOQVW010000000
VERSION     DAOQVW010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136695_length_1363_cov_2.428899"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKK85_08790"
     CDS             <1..254
                     /locus_tag="PKK85_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnosyl transferase"
                     /protein_id="HNS26150.1"
                     /translation="FVPFDALWLTYLDTGNYVWLNHKYTKSRWAFYRFLVEGYLYQFV
                     WIVLAGDHKYRRVSLLTQAWIDGWRGNFDNFKPRKILYS"
     gene            365..>1363
                     /locus_tag="PKK85_08795"
     CDS             365..>1363
                     /locus_tag="PKK85_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNS26151.1"
                     /translation="MVLNRKSRTTRLSSLDMLRGLAVLLMVEAHITIMIPFISELAGV
                     LAAPTFLFVAGLSFQLFLQNRESRALKSLDVFLEVFWRAVALFFITSLITWTANLVLG
                     WESSVFTNIFVIISTGFLVGYLVRRSFKGKVLAIILVLVLDLLIRGYNVEGLAFLAGY
                     GPGLQVSILPYLCYFFFGQLAYTAYKKENFNCYDNQILLVYALLFFLLNLAVYLTFPY
                     PLVSELRGYLPMLFMVASLLILLLLVLVRVVELDGKLKRWLRPLENLGHVSFTSYYIS
                     YASFILLGTLQFSQPVPANLLIFLATSGGLVLLEKHWRPSYRYGLEWWYRRVSAWGL"
BASE COUNT          273 a          351 c          368 g          371 t
ORIGIN      
        1 attttgtacc ctttgatgcc ctgtggttaa cctacctcga caccgggaac tacgtgtggc
       61 ttaaccataa atacaccaaa agtagatggg ccttttaccg ttttctggtg gaaggctacc
      121 tctaccagtt tgtatggatt gtgctggctg gtgaccataa ataccgccgg gtaagtctct
      181 taacccaggc ctggatcgat gggtggaggg gtaatttcga caactttaag ccccgaaaaa
      241 ttttgtacag ctaaggggtt tatcagccca cccctatata ataataagta gagtggattt
      301 tccatgatat tctgattaat aaaggtagga ggcctaagtt atcaagctga gaacccgggg
      361 tagaatggtg ctgaacagga aaagtagaac cacccgacta tcctccctgg acatgctccg
      421 gggcctggcg gttctgctca tggtggaggc ccacatcacc attatgatac ccttcatctc
      481 ggagttggca ggcgttttag ctgctccgac attcctcttc gtggcggggc ttagttttca
      541 gctctttcta cagaaccggg agagccgggc ccttaagagt ctggacgttt tcctggaggt
      601 tttttggagg gcggtggccc tctttttcat aaccagcctc atcacctgga ctgccaacct
      661 ggtgctgggc tgggaaagca gtgtattcac caacatcttc gtcatcatct ccaccggctt
      721 cctggtgggg tatctggtgc gccggagctt caagggtaag gtgctggcca tcatcctggt
      781 gttggtgctg gacctgttga tccggggcta caacgtggag ggcctggcct tcctggctgg
      841 ttatggtccg ggcctccagg tgagtatact gccctacctg tgctacttct tcttcggcca
      901 attagcctac acagcctaca aaaaggagaa cttcaattgc tacgataacc agatcctcct
      961 ggtctatgct cttctctttt tcctcctgaa tttggcggtg tacctgacct tcccctaccc
     1021 cctggtgagt gagttgaggg gctacctgcc catgcttttc atggtggcca gcctcctcat
     1081 attgctcctg ctggttctgg tgcgggtggt ggagttggat ggtaaactga aaaggtggct
     1141 gcggcccctg gagaatctgg gccatgtatc cttcaccagc tactatatct cctacgccag
     1201 cttcatactc ctgggcaccc tgcagttcag ccagccagta ccggctaatc ttctgatctt
     1261 cctggccacc agtgggggtt tggtcctgct ggagaagcac tggaggccca gttacaggta
     1321 tggattggaa tggtggtacc gcagggtatc agcctggggg ttg
//
LOCUS       DAOQVW010000480         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_137093_length_1360_cov_4.076628, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000480 DAOQVW010000000
VERSION     DAOQVW010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137093_length_1360_cov_4.076628"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PKK85_08800"
     CDS             complement(<1..713)
                     /locus_tag="PKK85_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNS26152.1"
                     /translation="MVGVPFINPLSLEGREMVRQLGSLEDLTHDNPELIGIVTRSKSQ
                     DISDDQDIPRNHLELALKRLEWYVKKRNNPNFNYRKYAFLFQGDIEKFDTISFYLLSQ
                     AIGVKFGPNSRESRLMAESQGSLIQNRLEELNTRNREEIVLKALQELLSQGTLKWTFF
                     EDLLSSRRIQLSDLVLDQGEVIMDQDDFRERLGPRLHHRDPDKMYQLLIGEELRELIL
                     IQMVMQQTENYMAEVHEKAR"
     gene            910..>1360
                     /locus_tag="PKK85_08805"
     CDS             910..>1360
                     /locus_tag="PKK85_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNS26153.1"
                     /translation="MNKAFITCALPYANGPCHLGHLRSTYIPADIYARYNRMKGREVL
                     FVCATDEHGTPIAVKAENEDKSPMEIAGRYYNLIKDDLDACHISFDNFSRTTDSLHYQ
                     IAQNFFLDLYQKGYIYEETIQQLYCPDCSRFLPDRYVVGSCPQCHAEG"
BASE COUNT          347 a          316 c          296 g          401 t
ORIGIN      
        1 cgggctttct catggacttc ggccatgtaa ttctcggttt gttgcattac catctggatt
       61 aggatcagtt cccgtagttc ttcccctatg agtagctggt acatcttatc cgggtccctg
      121 tgatggagtc gcggcccgag acgctccctg aaatcatcct ggtccatgat cacctccccc
      181 tggtccaaga ccagatcaga aagttgtatc ctacgggagc ttagcaggtc ctcgaagaag
      241 gtccacttaa gtgtcccctg gcttaaaagt tcctgtaagg cctttaacac tatttcttca
      301 cgattcctgg tgttgagttc ttccagccgg ttctggatga ggctgccctg ggattcagcc
      361 atcaaacgac tttcgcggga gttcggaccg aattttaccc ctatagcctg ggatagaagg
      421 taaaaggaga ttgtatcaaa tttttcaatg tccccctgga atagaaatgc atactttctg
      481 taattgaagt tagggttgtt acgcttcttt acgtaccact ccaggcgttt aagggcaagt
      541 tcaaggtggt tacggggtat atcctggtca tctgatatat cctgagattt gctacgggta
      601 actatcccta taagttcagg gttatcatgg gtcagatcct ccagactccc cagttgcctt
      661 accatttccc ggccctccag ggatagggga tttataaatg gaacacctac catgatctga
      721 tcttggtttt ataaaataat gtacttgatg tattttttta gagtatcaat tttatgctgc
      781 taaatttata tatacataat atgaacaaag ggagatttac cttttaatca gtactgatac
      841 tttctaaaat tataaaattc gttttacgag tttattatga tttgaaacat atatatggga
      901 ggaatgaatt tgaataaggc ctttatcacc tgtgctttgc cctacgccaa tggtccctgt
      961 cacctgggac atctcagatc aacctacata ccagccgata tttacgcccg atacaaccgt
     1021 atgaagggcc gtgaagttct ttttgtgtgt gccaccgatg aacacggcac ccccattgca
     1081 gtgaaggccg aaaatgagga taaatcacca atggagattg ctggacgata ttataacttg
     1141 ataaaagatg atctggatgc ctgtcacatc tcctttgaca acttctccag aaccaccgac
     1201 tccctgcact accagatcgc ccagaacttc tttttagatc tgtaccagaa gggctacata
     1261 tacgaagaga ccatacagca gctgtactgc cctgattgca gccgttttct gccggatcgc
     1321 tatgtagtgg ggagctgtcc ccagtgccat gcagaaggag
//
LOCUS       DAOQVW010000481         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_137355_length_1359_cov_2.162577, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000481 DAOQVW010000000
VERSION     DAOQVW010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137355_length_1359_cov_2.162577"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKK85_08810"
     CDS             complement(<1..315)
                     /locus_tag="PKK85_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26154.1"
                     /translation="MLAVDIILGVFIFFQREKLNESPFSTEFSFQFPFLAGSDSENNL
                     YLLDNGVKRLTKIKPDGEVVFSLNGGKRGEGEFYQAWKMAIDKKDRVYIFNGVRNIKD
                     GLT"
     gene            complement(338..562)
                     /locus_tag="PKK85_08815"
                     /pseudo
     CDS             complement(338..562)
                     /locus_tag="PKK85_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019267068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     gene            complement(701..1006)
                     /locus_tag="PKK85_08820"
     CDS             complement(701..1006)
                     /locus_tag="PKK85_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNS26155.1"
                     /translation="MEAVKIMGDDLLVDIEVTPRSHHFGLAGYNAWRKTLEVKIKSPP
                     IKGKANKEIIKEFSQLTGCPVELESGLKSQQKTIRIRGMSKKDFLKIVDSHCEAKSG"
     gene            1144..1230
                     /locus_tag="PKK85_08825"
     tRNA            1144..1230
                     /locus_tag="PKK85_08825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1178..1180,aa:Leu,seq:gag)
BASE COUNT          379 a          285 c          260 g          435 t
ORIGIN      
        1 ggttaaaccg tctttaatgt ttctgacacc gttaaaaata taaacccggt cttttttatc
       61 aatcgccatt ttccatgcct gataaaactc gccctctccc cgtttccctc cgttgaggct
      121 gaaaacaact tccccgtcgg gcttgatttt agtgagccgt ttcacaccgt tgtctaatag
      181 ataaaggttg ttttcagagt ccgagcctgc taaaaaagga aattgaaatg aaaattcagt
      241 gctgaaagga gattcgttaa gtttttcccg ctgaaaaaag ataaaaactc cgagaatgat
      301 atccactgca agcacaagaa tcaattttac cgcaattttt caggaaaaac tccagctcga
      361 aggaacgatc aaagtgatcc tcaatggaac gtttcccctt accagtgatg attagaatat
      421 catcaattcc ggaagccact gcttcctcaa ccacgtactg aatggtgggt ttattaaata
      481 caggtaacat ttctttgggt tgagccttag tagccggtaa aaatctggtt cccaatcctg
      541 cagcaggtat tacagctttc ataatagata ccccctaaag gcattaaact agtttgatct
      601 gttctacagt ggtgatctta tttatttaat ctctctttca attatatcaa attataggtg
      661 tcatccggga taattagtgg gaagttgaat atctggttat ttaaccggat ttagcttcac
      721 aatgagagtc cacgatttta agaaaatctt ttttggacat tcccctaatt cttatggtct
      781 tttgctgact cttaagtcct gattccaatt caactgggca gccagttaac tgggagaact
      841 ctttgattat ctctttattg gcctttccct taattggagg ggattttatt ttcacttcca
      901 gggttttcct ccaggcattg taaccggcca ggccgaaatg gtgggaacgg ggtgtaactt
      961 cgatatcaac caataaatca tcccccataa tcttcaccgc ctccatatct tcacccctat
     1021 gataaatatt tgagtggccc ttaagtatta agtttgggtg attaaagtgg ggcaacattt
     1081 aaatagggaa agaaaaaaag tagggtatgc tctattaatt ctcggaatca attaatatta
     1141 aatgcagggg tggtcgagcg gtcaaaggcg ctaggttgag ggcctagtgg gtgagtccct
     1201 tcgcgggttc gaatcccgtc ccctgcacca ttttaacctg tttttataaa tttatttatt
     1261 ctaatctaag gctattcatg gctttcatca attatgatct tcacttccag tactttgcca
     1321 tcctttaaat cttctattaa aatggggtcc aggtcacat
//
LOCUS       DAOQVW010000482         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_137565_length_1358_cov_1.406754, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000482 DAOQVW010000000
VERSION     DAOQVW010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137565_length_1358_cov_1.406754"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>896
                     /locus_tag="PKK85_08830"
     CDS             <1..>896
                     /locus_tag="PKK85_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNS26156.1"
                     /translation="IDSAHKQGYTFLEGHLHECLGELLLCYGQCSEEMYFNQAARLYK
                     NCGAERKEFSLLKRYPTYFEDDISYPQIEVEEPPLTLPDIDIEYLMKSALAISAEIEL
                     EALLKKIMNVVIESSGAQSGYLIMEEEDGLFIHAESHATFKEDVQISKKKLETAGGIC
                     KAIVRYVQRTGERLILDNASEEGEFKDNREVQDMKLRSVLCMPVFKQSKMIGILYLEN
                     RLANSVFTPEKTELLELLISQAAISLENATLFVKREKTGKKLEKTLEELKRSNKELQQ
                     FAYIASHDLQEPLRMISSFLQL"
     assembly_gap    897..906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <907..>1358
                     /locus_tag="PKK85_08835"
     CDS             <907..>1358
                     /locus_tag="PKK85_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS26157.1"
                     /translation="YEGQLDSDADEFIDYAVDGAARMQKLINDLLAYSRVNRTTSEFE
                     KVDLEKVLDEILMYHKLSIKENDVYVTRDPLPMVNGDYSQMVQVFQNLVGNAIKYRGE
                     ESPKIHISAEKEDDNWLFKVEDNGIGIDPKYFERVFMIFKRLHTNEEY"
BASE COUNT          421 a          270 c          322 g          335 t
ORIGIN      
        1 catagattcc gcacataagc agggctacac cttcttggag gggcatctcc atgaatgtct
       61 gggggagctt ctactctgct atggacagtg ttctgaagag atgtatttca accaggcagc
      121 gcgcctgtat aaaaattgtg gagcggaaag aaaagagttc agcctcttaa aaaggtatcc
      181 tacctacttc gaggatgata tttcttatcc acaaatcgag gtggaagaac ctcccctcac
      241 cctcccggat attgacattg aatacctgat gaaatccgca ctggctattt ctgcggagat
      301 agaactggaa gcattactta agaagatcat gaacgtggtc atcgagagtt ccggtgcaca
      361 aagcggatac ctgatcatgg aagaggaaga cggtcttttt atccatgctg aaagccatgc
      421 cactttcaag gaggatgtgc agatttccaa gaaaaaactg gaaacagcag gtgggatctg
      481 caaagccata gtccgttatg tgcaacgtac aggagaacgt ttgattcttg ataatgcctc
      541 agaagaaggg gaattcaaag ataacaggga agttcaggat atgaagttgc gttcagtcct
      601 ttgtatgcct gtatttaagc agtcaaagat gattggaata ctttacctgg aaaatcgtct
      661 ggcaaattcc gtgttcactc ctgaaaagac ggagctttta gagttactga tatcacaggc
      721 ggctatctcc ctggaaaatg ctacactatt tgtaaaacgt gaaaaaacag ggaaaaaatt
      781 ggaaaaaacc ctggaagagc tgaaacgttc caacaaggag cttcaacagt tcgcctacat
      841 cgccagccac gacctgcagg agccactgag gatgatatcc agttttttac agcttcnnnn
      901 nnnnnngtta tgagggccaa ttagattcgg atgccgatga gtttatagat tacgctgtag
      961 atggggcagc acgcatgcag aagttaataa acgatcttct ggcttattct cgggtaaata
     1021 gaaccaccag cgagttcgaa aaagtggacc tggaaaaagt cctggacgaa attttgatgt
     1081 accataaatt atcgatcaaa gagaacgacg tgtatgtaac tcgtgaccct ttaccaatgg
     1141 ttaacggtga ctactcccag atggtacagg tgttccagaa tctggtggga aacgccatta
     1201 aataccgtgg cgaagaatca ccgaaaatcc atatatcagc cgagaaagag gatgataact
     1261 ggttgtttaa agtggaagat aacggcatcg gaatcgaccc taaatatttt gagcgtgttt
     1321 ttatgatttt caaacgtctg cataccaacg aagagtac
//
LOCUS       DAOQVW010000483         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_137792_length_1356_cov_2.902383, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000483 DAOQVW010000000
VERSION     DAOQVW010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137792_length_1356_cov_2.902383"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..>1356
                     /locus_tag="PKK85_08840"
     CDS             179..>1356
                     /locus_tag="PKK85_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNS26158.1"
                     /translation="MNPLIALMIIVPIICALFLNLLHKKDRTIKAIAIVMAITLPLLP
                     LLANYGLHYFGGYEPLYQNPALSKGLPSLITGTALNTFHPAITYSFQDAQKLFIFILG
                     LVAFLAIIVSLFETRRPSGVYGFMMFMGVAAVSALILTDDIFNFYVFFEIAALATVGV
                     VLVSQVKGNYETALKYMILGSIASPILLLGIALLLGVTGNVNITDIILSLKSGAVNPQ
                     NPVILMACGMIFFGWLYGSGLPPFHTIKSAIYSKALPHGAALLQAFSVFTFIALGIII
                     LRIFSYLPLSQLVIIGVSLLAMIMGITMAILQTDLKRMIGFLAVGELGYIGLGLGLGT
                     ALGITAGLFQAVNEVVITAFLFIGFGTIYYQSGESDIRKLGGFMVKNPKVALLVLLSG
                     "
BASE COUNT          354 a          314 c          294 g          394 t
ORIGIN      
        1 tgtaattggt gccagtacaa tggcagttat gttaggcatt atcatggtgc tctacaagaa
       61 acacggatcc ctaagggcat ccaaggtact gggggaatag atttcatgtt gataaaagtt
      121 atcgttactt ggaaacccca aataacgagg atgattacaa ttttagggag gcaataaagt
      181 gaacccctta atagctttaa tgatcatcgt tcccataatc tgcgctctct ttttaaacct
      241 tttacataaa aaagaccgca caataaaggc tattgcaatt gtcatggcaa ttacactgcc
      301 attactacca cttttggcca attatgggct gcactacttc ggagggtatg agccactgta
      361 tcagaacccc gccctgtcca agggcttacc atctttgata actggaactg cccttaacac
      421 cttccacccg gccattacct actccttcca ggatgctcag aaactcttca ttttcatctt
      481 ggggctggtg gccttcctgg ccataattgt ttcactcttt gaaacccgga ggccatcggg
      541 agtgtatggc ttcatgatgt tcatgggggt ggcggcggtg agcgccctca tcctaactga
      601 tgatattttc aacttctacg tgttctttga aatcgcggca ctggccacgg tgggagtggt
      661 cctggtatcg caagtgaagg gaaactacga aaccgcgttg aaatatatga tcctgggaag
      721 tatagcttcc cccatacttc tattggggat cgcactgctt ttaggagtaa caggtaacgt
      781 aaacattacc gacatcattt tatcattaaa aagtggagcg gtaaatcctc aaaatccggt
      841 gatcttaatg gcatgtggaa tgattttctt tggatggctc tatggctcgg gacttccacc
      901 attccacacc atcaaatcag ctatatacag taaagccctg ccacacggag cagcactcct
      961 ccaggccttt tctgtcttta ctttcatagc cctgggcata atcatactcc gtatcttttc
     1021 ctacctgccc ctgtcccaac tggtaataat cggagtatct ctcctggcca tgatcatggg
     1081 gatcaccatg gccatattac aaactgatct taaacgtatg attggttttt tggcagtagg
     1141 agaactaggc tacattgggc tgggcttagg actgggcaca gccttaggaa taactgctgg
     1201 actcttccaa gccgtaaacg aggtagtaat aactgccttt ctctttatag gatttggaac
     1261 aatttactac cagtccggtg agagtgatat tcgtaaatta ggcggattta tggtgaaaaa
     1321 ccctaaagtt gcacttttag ttcttctatc cggatt
//
LOCUS       DAOQVW010000484         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_137986_length_1355_cov_2.249231, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000484 DAOQVW010000000
VERSION     DAOQVW010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137986_length_1355_cov_2.249231"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="PKK85_08845"
     CDS             complement(<1..1343)
                     /locus_tag="PKK85_08845"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS26159.1"
                     /translation="MPVYMELSDLVRPDGWSKRRIDVTIITSGLFRDLYSRQSQLLDN
                     AFRVALARSYYTIINDANLKAKYGTRLNTALDPIMKDIGYYGVGYESLNDNYVAQHWV
                     EDYAYYLSLNMTPEGAGEMAITRIFAPPEGDYGAGISKLVSESWTWDDRMELGEFYLK
                     RMANMYSHKNWGTNNVAVFSRALTGISTIFTSRNTNLYGVLDNDDFFDYWGGLSMALE
                     YVNGKAPTMYVLDYSNKAKPDSISLEQYMNRELTTRYYNPDWIRGMMNEGYSGARYMS
                     NKFVKNLLGWQMTRPGSVQNWMWDKVADVYLRDSYNMGVTQFLSSGNNAYSMISLTGA
                     MLTAAFEGYWTPDAATMNLVANTWAQMIAANGVACCDCSCANLAMMKWASSFVNTDLL
                     AQLNAQFYEATQQAQFAPGETPTPTPNPQPGQPSPGSQGTTGTSSSESSSAGDAAG"
BASE COUNT          270 a          371 c          392 g          322 t
ORIGIN      
        1 ccagcagcat ccccggcgga gctgctctca gaggaagaag tgccagtggt gccctgggat
       61 ccgggtgagg gttggcctgg ttgggggttg ggagtagggg ttggtgtctc tccaggggcg
      121 aactgggcct gctgggtggc ctcatagaac tgggcattca actgggccag taaatcagtg
      181 ttaacgaaac tggaggccca tttcatcatg gccaggttgg cgcagctaca gtcacagcag
      241 gccaccccat tggcggcgat catctgggcc caggtgttgg ccaccaggtt catggtggct
      301 gcgtcggggg tccagtagcc ctcgaaggcc gcagtcaaca tggctccggt gaggctgatc
      361 atggagtagg cgttgttacc cgaacttaaa aactgggtta ctcccatgtt gtagctgtcc
      421 ctgaggtaca cgtcagccac cttatcccac atccagttct gcacagaacc aggccgggtc
      481 atctgccagc ccaggaggtt cttcacgaac ttgttggaca tgtaccgtgc cccactgtag
      541 ccttcattca tcatgcccct gatccaatcc gggttgtagt agcgggtggt gagctcccgg
      601 ttcatgtact gttccaggct gatggaatct ggcttggcct tgttggagta gtccaggacg
      661 tacatggtag gggcctttcc attcacgtat tccagggcca tggacagtcc tccccagtag
      721 tcgaagaagt cgtcattgtc caaaaccccg tagaggttgg tgttacgact ggtgaagatg
      781 gtgcttatac cagtcagggc acggctgaac acagccacgt tgttggttcc ccagttcttg
      841 tggctgtaca tgttggccat acgcttgagg tagaactcgc ccaactccat gcggtcatcc
      901 caggtccagc tctcagagac gagtttgctg atacccgccc cgtaatcccc ctcagggggt
      961 gcgaagatcc tggtgatggc catctcccct gccccctcgg gtgtcatgtt cagggacagg
     1021 tagtaggcat agtcctccac ccagtgctgg gccacgtagt tgtcattgag ggactcgtat
     1081 ccaacaccat agtagccgat atccttcata atgggatcca gggcggtgtt cagcctagta
     1141 ccatatttgg ctttcaggtt ggcatcgttg atgatggtgt agtagctccg ggccagggcc
     1201 acccggaagg cattatctaa aagctgggac tgcctgctgt aaaggtcccg gaagaggccg
     1261 cttgtgatga tggttacatc gatcctcctc ttactccaac catccggcct gaccaggtca
     1321 gataactcca tataaaccgg catctccacc atttt
//
LOCUS       DAOQVW010000485         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_138076_length_1354_cov_4.252502, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000485 DAOQVW010000000
VERSION     DAOQVW010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138076_length_1354_cov_4.252502"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1354)
                     /locus_tag="PKK85_08850"
     CDS             complement(<1..>1354)
                     /locus_tag="PKK85_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HNS26160.1"
                     /translation="VPSIWSGDIESEEGQGMVNTDPDGPLAVMITDVSIDKHAGEIAT
                     GRVYGGTIEKGSEIFFVGSHGKARTQQVGVYFGPERVNTDKVPAGNIVAITGARNAVA
                     GETISSVDRKIEAFEGLEHISEPVVTVAVEAKNTKDLPKLIEVLRQVGKEDPTVKMQI
                     NEETGEHLVAGMGELHLEIIAYRINEKGVEIETSEPIVVYRETIAGTAGPVEGKSPNK
                     HNRFYIEISPLPDSVYQAIQEGTIKEGKVKGKENASIFIEAGLPKDEARKVWDVYEKS
                     VFINATRGIQYLDEIKELLLEGFESAMDDGPIARERVMGLKIRLVDAKIHEDAVHRGP
                     AQVLPAIRKAIYGAIMMAEPVLLEPVQKVFINVPQDYMGSSTREVQNRRGQIIDMGQE
                     GDMATLESKVPVAEMFGFAGDIRSAAEGRCLWSTENAGFERLPKELQKTIIREIRNRK
                     G"
BASE COUNT          259 a          390 c          308 g          397 t
ORIGIN      
        1 cccttccggt ttctgatctc cctaattatg gtcttctgaa gctccttggg cagtctttca
       61 aatcctgcat tctctgtgga ccacaaacat cttccttcag cagcagatct tatgtcccct
      121 gcgaatccga acatctccgc tacaggtacc tttgattcca gtgtggccat gtctccttcc
      181 tggcccatgt cgatgatctg accgcgacgg ttctggactt cgcgagtgga tgatcccata
      241 taatcctggg gcacgttgat aaacaccttt tgaaccggtt ccaggagcac tggttcagcc
      301 atcatgatgg ctccataaat ggctttacga atggcaggca gtacctgggc tggaccgcgg
      361 tgtaccgcgt cttcgtgtat tttagcgtct actaacctga tttttagtcc catcaccctt
      421 tctcgagcta tggggccgtc gtccatggca ctttcaaatc cttccaatag gagttccttg
      481 atctcgtcca ggtactggat accacgtgtg gcgttgatga aaacgctttt ctcataaaca
      541 tcccacacct tccgagcttc atccttaggc aaaccagctt ctatgaagat ggaagcgttc
      601 tctttaccct tcaccttacc ttccttgatg gttccttcct ggatggcttg atacacggag
      661 tctggcagag gtgatatttc gatgtagaag cggttgtgtt tgttggggga tttaccctcc
      721 actggaccgg cagttccagc aatggtttcc cggtacacta caattggttc cgaagtctcg
      781 atctccactc ccttctcgtt gatacggtag gcgatgatct ccaggtggag ttcacccatg
      841 cccgccacca ggtgttcacc ggtttcctcg ttgatctgca tcttgacggt ggggtcttct
      901 ttacccactt gccgcagtac ttcgatgagt ttaggtaagt ccttggtgtt tttggcctcc
      961 acagccaccg taaccactgg ttcggagatg tgttccaggc cctcgaaggc ctcgattttt
     1021 cggtccacac tggaaatagt ttccccagct actgcgttgc gggcaccagt gatggctact
     1081 atattcccag caggtacttt gtccgtgttt actcgttctg gaccgaagta aactcccact
     1141 tgctgggtcc gggcctttcc atgggatcct acgaagaaga tttcactacc cttctctatg
     1201 gtaccaccgt acacccgtcc ggtggcaatt tcaccagcat gtttgtcaat actgacatca
     1261 gttatcatca ctgccagggg tccgtcaggg tctgtgttca ccataccctg tccttcctca
     1321 ctctcgatgt ccccggacca tatgctgggc accc
//
LOCUS       DAOQVW010000486         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_138175_length_1354_cov_2.033102, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000486 DAOQVW010000000
VERSION     DAOQVW010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138175_length_1354_cov_2.033102"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PKK85_08855"
     CDS             <1..1285
                     /locus_tag="PKK85_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNS26161.1"
                     /translation="LLTIIVICFGITWITSTLGLSLALGAFLAGLTISNTEYKHQALG
                     NILPFQDIFLSLFFISIGMLLNISFVLENLPLIILLTIAVILLKSVIAGVVAGILGLS
                     FRVMVLVGLMLSQIGEFSFILAAVGVQYNLITNPFFQTILAVTILTMSLTPFFKDRAH
                     QISDILEKVLPVPDRVLSGTYSIPQPQKVPPADHLIIVGFGVNGKNMARAASQAKIPY
                     VVAELNPQIVRRKKVMGEPICYGDASQPTVLNQLNITEARVMVIAISDPVGSRKIVNM
                     ARKMNPTLYIIVRTRYIYEMEELHNLGADEVIPEEFETSVEIFTRVMNQYHLTDDEVH
                     KFVDELRADEYEMFRTLASWEINTCSVNDTLTDIQVSSFTIEEDVKLGDLCLNDQDLT
                     PIAVARKDQTIRDLEDNFQLKPDDVLIYTRKDDGS"
BASE COUNT          406 a          290 c          285 g          373 t
ORIGIN      
        1 cctcttaacc atcatcgtca tatgttttgg aattacctgg ataacctcta ctctaggatt
       61 atctctggca ttaggtgctt ttctggctgg tcttacaata tcaaatacag aatacaagca
      121 tcaggccctt ggaaatattc taccctttca ggatatattt ttaagcctat ttttcatctc
      181 cattggtatg ctcttaaata ttagcttcgt gttagaaaat ttacccctta taatcctctt
      241 aactatagct gtaatcctat taaaatcggt gattgccgga gttgtggctg gaattttagg
      301 actgtccttc cgggtcatgg tactggtggg gttgatgttg agccagatag gggagttctc
      361 ttttatccta gcagcagttg gagtacagta caacctgata accaacccat tcttccagac
      421 catcctggcg gtcacaatct tgactatgtc cctgactccc ttcttcaagg atagggcaca
      481 ccagatatct gacattctgg aaaaggtgtt accggtcccg gatcgagtcc tgagtggaac
      541 atattcaatt ccccagcctc agaaagttcc tccagcagat catctgatca tagttggatt
      601 tggtgtcaac gggaaaaaca tggctcgagc tgcttcacag gcaaaaatcc cctacgtagt
      661 ggccgagctt aacccacaga tcgtgagaag gaagaaggtg atgggggagc ccatatgtta
      721 tggtgatgct tctcaaccaa ctgtattaaa tcaattaaac atcacagaag ccagggtcat
      781 ggtgatcgcc atctcggacc ctgttggaag ccgtaaaata gtaaacatgg ctagaaaaat
      841 gaatccaacc ctttatatca tagttagaac ccgttacata tatgaaatgg aggaattaca
      901 caatttagga gctgatgagg taatacctga ggaatttgaa acttcagtag aaatattcac
      961 cagggtgatg aatcaatacc acttaacaga tgatgaggtc cataaatttg tggatgaact
     1021 ccgggccgat gaatacgaga tgttcagaac acttgccagc tgggaaatca acacctgcag
     1081 cgtcaatgac acgttaacag atatacaggt ctcttcattc accatagaag aagatgttaa
     1141 gctgggagat ctctgcctta atgatcagga cttgacacct atcgccgtgg cccgcaaaga
     1201 tcagaccata agggacctgg aagataactt ccagctgaaa ccggacgatg tgttgatcta
     1261 taccaggaag gatgatggaa gttagatatt tctaaaaaaa tgggaaaatt gaaattccac
     1321 catcaatata tcattccacc atcaatatat catt
//
LOCUS       DAOQVW010000487         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_138413_length_1352_cov_3.414803, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000487 DAOQVW010000000
VERSION     DAOQVW010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138413_length_1352_cov_3.414803"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKK85_08860"
     CDS             <1..430
                     /locus_tag="PKK85_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HNS26162.1"
                     /translation="LLKALLRHLILKKDEIILLDMEAGIEHLGRRTAEAVDLMIIVVE
                     PGLKSLETATRIRKLAKEIGVDEVVAVINKVSNEDEEAFVTSKLNEIGLNVLGSIPHD
                     YNVVRADMEGQPLVNYPDSSALKAVEDISENILSNLPQLS"
     gene            432..668
                     /locus_tag="PKK85_08865"
     CDS             432..668
                     /locus_tag="PKK85_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HNS26163.1"
                     /translation="MQITARLVFHYQEEEQALIAYQSLEPDNRNYLESKVVDNDLVCK
                     IRGESVPRILATLDDLIFSEILVEKVLEIDGDEI"
     gene            665..>1352
                     /locus_tag="PKK85_08870"
     CDS             665..>1352
                     /locus_tag="PKK85_08870"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032078.1"
                     /note="catalyzes a two-step reaction, first charging a
                     serine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNS26164.1"
                     /translation="MKFTLKGQITFSKDAEEARADIKQFLDDANQNLFLKGVPLEQKE
                     DAARVVEWTLDSSTLYLKLVSGRRGRAHDALLRIKKPLTQLLGSKYRLGIRRISVDFY
                     EIRIPTPEMVDVGEMPYVQDSSFKEGVMIIKFQKLDEADIRRHVVDRVVKQVQSQARA
                     SVEGSETDILTRQVTKVEPGTIVFQGKKQEIYFDGDPTEELARRGWVKKFPGKGQWFY
                     AFNLLCELDQP"
BASE COUNT          430 a          271 c          321 g          330 t
ORIGIN      
        1 acttcttaaa gcacttttaa gacaccttat actgaaaaaa gatgaaataa tccttttgga
       61 catggaagca ggtattgaac atctggggag gcgtaccgca gaagccgtgg atctgatgat
      121 catcgtggtg gaaccaggac tcaaatcctt ggaaaccgcc acccgtatca ggaagctggc
      181 taaagaaatt ggagtggatg aagtagttgc cgttataaat aaagtttcca atgaggatga
      241 agaagcattt gtaaccagca aactcaatga aattggttta aatgtattag gaagtatccc
      301 ccatgattat aacgttgtgc gagccgatat ggaaggtcag cccctggtga attatcctga
      361 ttcttcagct ctaaaagcag tggaggatat ctcggaaaat attttaagca atctacccca
      421 attatcatag tatgcagatc actgctaggc tagtcttcca ttatcaggag gaagaacagg
      481 ccctaattgc ttaccaatca ttagaacccg ataacaggaa ctatctggag tcaaaagttg
      541 tagataatga ccttgtatgc aaaattcgtg gcgaatctgt accaaggata ttggcaaccc
      601 ttgatgacct tattttttca gaaatcctgg tagaaaaagt gttagaaata gatggtgatg
      661 agatatgaag tttacactaa agggccagat caccttcagc aaagatgctg aagaggcccg
      721 tgctgatatc aaacaattcc tagatgatgc taaccaaaac cttttcctca aaggagtgcc
      781 cctagaacag aaggaagatg ccgctcgtgt ggtggaatgg actctagata gcagcaccct
      841 gtatcttaag ctggtctccg gtagaagagg aagagcccac gatgcccttt taaggataaa
      901 aaaaccatta acccaacttt taggatccaa gtaccgacta ggaatccgta gaatttctgt
      961 ggatttctat gaaatacgca tacccacccc ggaaatggtg gatgtaggtg aaatgcccta
     1021 cgttcaggat tccagcttca aagagggtgt catgataatt aaattccaaa aattggacga
     1081 agcagatatc cgccgccatg tagttgatcg agtggtgaaa caagtccaga gccaggccag
     1141 ggcctcggtg gaaggatcag aaaccgatat tctgactagg caggtaacca aagttgaacc
     1201 aggaaccata gttttccagg gaaaaaaaca ggaaatctat tttgatggtg atcccactga
     1261 agagttagcc cggcggggat gggttaaaaa gtttccgggc aaagggcagt ggttctatgc
     1321 gttcaacctc ctctgcgagc tcgatcaacc gg
//
LOCUS       DAOQVW010000488         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_138462_length_1352_cov_2.589052, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000488 DAOQVW010000000
VERSION     DAOQVW010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138462_length_1352_cov_2.589052"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..164
                     /locus_tag="PKK85_08875"
     CDS             3..164
                     /locus_tag="PKK85_08875"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNS26165.1"
                     /translation="MSHANNLEVSYVVLVGARDLEQGQVTVKDMESGQQELVALEDVV
                     DFLVERIRG"
     gene            177..578
                     /gene="hisI"
                     /locus_tag="PKK85_08880"
     CDS             177..578
                     /gene="hisI"
                     /locus_tag="PKK85_08880"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNS26166.1"
                     /translation="MDLSRFNFRHRLGGEKLLTAVAQDYQSGEVLMVAFMNQEALKFT
                     LETGVAHYWSTSRNRIWLKGESSGHQQLVEEILVDCDADALVLKIKQEGAACHEGYYS
                     CFFRKVDFDDGFKLKIIRDKVFEPEQIYKEG"
     gene            581..>1352
                     /locus_tag="PKK85_08885"
     CDS             581..>1352
                     /locus_tag="PKK85_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNS26167.1"
                     /translation="MKIVPDTSVIVDGRITRIVQDDEYKGCEVIVSEAVVAELENQAN
                     KGRETGFNGLEELKNLQKLHSEGRIHVSYVGRRPTLDEISLARGGEIDAMIRSTAGEY
                     QATLMTSDRVQKEVAEAQGLKAVYLKPEMLEYRDIEVTRFFDKNTMSVHLKENVIPMA
                     KKGTPGNIQLVKIGSRPLKRSKIESMAREIVERAKSDFQSFIEIEWEGATVVQFREYR
                     ISIARPPFSDGVEITIVRPVAKVSIGSYHLPDKLIDRLE"
BASE COUNT          363 a          280 c          405 g          304 t
ORIGIN      
        1 taatgtccca cgccaacaac ttggaggtga gttacgtggt gttggttggt gcccgggact
       61 tggagcaggg ccaggttaca gtgaaggata tggaaagtgg ccagcaggaa ctggtggccc
      121 tggaggatgt ggtggatttt ctcgtggaaa ggatcagggg atgatttttt atggcgatgg
      181 atttatctag atttaacttc cggcaccggc tcggtggtga aaaactcctt acagctgtgg
      241 cccaggacta ccagagcgga gaggtgttga tggtggcatt catgaaccag gaagccctga
      301 aatttaccct ggaaacagga gtcgcccatt actggagcac cagtcgtaat aggatctggc
      361 ttaaagggga aagctccgga caccagcagc tggtggagga gatcctggtg gactgtgacg
      421 ccgatgccct ggtcttgaag attaaacagg aaggagcagc ctgccatgaa ggatattatt
      481 cctgcttttt caggaaagtg gactttgatg atgggtttaa acttaaaatt atcagagaca
      541 aggtttttga accagaacaa atctataaag agggatagta ttgaaaattg ttcctgatac
      601 cagtgtaatc gttgatggcc ggatcacccg tattgtccag gatgatgaat acaagggttg
      661 tgaggttatt gtctccgagg cagtggtagc tgaattggaa aaccaggcca ataaaggaag
      721 agaaactggt tttaatggat tggaagaact taaaaatctg cagaagcttc atagtgaggg
      781 gcgtatacac gtcagttatg tggggcgccg gcccaccctg gatgagattt ccctggcccg
      841 ggggggtgaa atagatgcca tgatccgcag caccgccggg gagtatcagg ccaccctcat
      901 gaccagtgac cgggtgcaga aggaagtggc cgaggcccag ggactgaaag cagtttactt
      961 gaagccagag atgttggagt accgggacat tgaggtaacc cgctttttcg acaagaacac
     1021 catgtccgtc cacttgaagg agaacgtaat ccccatggcc aagaagggaa ccccgggcaa
     1081 tatacagctg gttaagatcg gttccaggcc acttaaacga tccaagatag agtccatggc
     1141 ccgggagata gtggaaaggg ctaagagtga ctttcagagt ttcatagaga tagaatggga
     1201 gggtgccact gtggtgcagt tccgggagta ccggatatcc attgcccgac cacccttctc
     1261 agatggggtg gagataacca tagtccggcc agtggccaag gtatccatcg gcagttacca
     1321 tcttcctgat aaactcatcg accgcctgga ga
//
LOCUS       DAOQVW010000489         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_138586_length_1351_cov_3.881173, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000489 DAOQVW010000000
VERSION     DAOQVW010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138586_length_1351_cov_3.881173"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKK85_08890"
     CDS             complement(<1..439)
                     /locus_tag="PKK85_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNS26168.1"
                     /translation="MSKGMDVAVLGTCNLDFISRVSRFAGADEEVNMDQLHQSTGGSA
                     ANSASTLSELGIKTGVISRVGEDYGGNLIRQSLTDKGIGTERIVSLDKPTGMAFIAVD
                     DQGERSIYVHMGANAHFQLLDEDRTYIEDAKALHLTGMYWEVVE"
     gene            complement(501..1289)
                     /gene="minD"
                     /locus_tag="PKK85_08895"
     CDS             complement(501..1289)
                     /gene="minD"
                     /locus_tag="PKK85_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HNS26169.1"
                     /translation="MARFIAMASGKGGVGRTSLAFNLGVALSLFGEEVVMLDMDLVMA
                     NMDVITGLLNPEVTLHDVLVRDKSIEDCVYEMNYGIRVVPTGMHFETLKNINTNYISW
                     NKIIKEIADYGNIFVLDLPAGIDSNILEALPADSEIILVTNSTMPSVADALKLRILLN
                     ELNLDILGFVLNMWYEDNFLLSPQEIESLLEIPMISIVPYDREMDRALALGRSVVEIN
                     STSPTSNAIMQLAADILGKEYQPVEPDKQSILNRIKKFVGMLPE"
BASE COUNT          329 a          342 c          304 g          376 t
ORIGIN      
        1 cttccaccac ctcccagtac atgcctgtaa ggtgcagggc cttcgcatcc tcgatgtaag
       61 ttctgtcctc gtctagaagc tggaaatggg cgttagcccc catgtgtaca tatatggatc
      121 tttctccctg gtcatccacg gcaatgaagg ccatcccggt gggtttatcc agggatacga
      181 ttctttcggt gccgatcccc ttatcagtta gactctgacg gattaggttt cccccgtagt
      241 cctcccccac ccgggatatt accccggttt tgatccccaa ttcagaaagg gttgaggctg
      301 agttggcagc ggatcctcca gtggactggt gtaactggtc catatttact tcttcgtcgg
      361 caccagcgaa tctggaaacc cggctgatga agtccaggtt acaggttcct aaaaccgcca
      421 cgtccatacc tttactcatg ctgatgcccc ctctagggga tagaataaaa tgttaggttt
      481 aaaataatct agatctttgg ttactccggt agcatgccca caaatttctt gatacggttc
      541 aggatgcttt gcttatctgg ctccactggt tgatattctt tccccaggat atcagcggca
      601 agttgcatta tggcgttgct ggtgggggag gtactgttga tctccactac ggagcgtccc
      661 agtgcaagtg cccggtccat ctcccggtcg tagggtacga tggagatcat gggaatttcc
      721 aggagggatt caatctcctg gggggagagc aggaaattgt cctcgtacca catgttcagt
      781 acaaaaccca gtatgtcaag gttcaattca tttagaagta tgcgcagctt cagggcatcc
      841 gccacagagg gcatggttga gttggtcacc aggattatct cagaatccgc aggaagggct
      901 tctaagatgt tagaatctat tccagctggt aagtccaaca cgaagatgtt cccataatca
      961 gctatctcct tgatgatctt gttccaggag atgtagttgg tgtttatatt tttcagggtt
     1021 tcaaagtgca tccctgtggg aaccacccgg ataccatagt tcatttcata aacacaatct
     1081 tcaatggatt tatctctgac taaaacgtcg tgaagggtaa cttcaggatt taacaatcca
     1141 gtgataacat ccatattggc catcaccaaa tccatatcca gcataaccac ttcttcgcca
     1201 aataggctca gtgccactcc caagttaaag gccagggaag ttctgcccac accgccttta
     1261 ccagaagcca ttgcaatgaa tcttgccatt ttaatcagta ctatttactt aacgccgtct
     1321 tcccattaat ccttccacca atttggagat a
//
LOCUS       DAOQVW010000490         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_138593_length_1351_cov_3.625772, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000490 DAOQVW010000000
VERSION     DAOQVW010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138593_length_1351_cov_3.625772"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKK85_08900"
     CDS             <1..386
                     /locus_tag="PKK85_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNS26170.1"
                     /translation="IRSIPQEYKEASLALGATHWQTIKNVIFPAAIPGVITSVILGMG
                     RAIGETLAIIMVAGNVVQIPGSIMDPVRALTSNIAIEMGYATGIHYNALFATGIVLIF
                     MIIILLVLANYLHYKKKVTVGGGYL"
     gene            471..1280
                     /gene="pstA"
                     /locus_tag="PKK85_08905"
     CDS             471..1280
                     /gene="pstA"
                     /locus_tag="PKK85_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNS26171.1"
                     /translation="MDLIFWASGIITLLILLMVIGYVFYKGLPVINLEFLFTDPMDSG
                     KAGGIFPMIVSSLYVTFIAVLVSAPIGVGAALYLAEYAGENRTVRLIRFGAETLSSIP
                     SIIFGLFGLAFFVIYLRMGWSVLSGGLTLALMALPTILAASEVSIESINKSYAQGSLA
                     LGATKWQTVSKVVIPAALPGITTGIILGMARAIAEAAAILYTVGAALITPISLLDPAR
                     PLPLHLYILATEGISLDNAWGTAAVLVILILIITVTTNTLVDRYRKQMMGR"
BASE COUNT          365 a          307 c          324 g          355 t
ORIGIN      
        1 ccattagatc tattccccag gaatataaag aagcttcgtt ggcactggga gccactcact
       61 ggcagaccat caaaaatgtc attttcccag ctgccatacc aggagtgata acctcagtaa
      121 ttctggggat gggacgcgca attggtgaaa cactggccat aattatggtg gcgggtaacg
      181 tggtacagat accaggttcc ataatggacc cggtaagggc attgacctct aatattgcca
      241 ttgagatggg ttatgccacg ggtatacact acaacgccct gtttgccaca ggcatcgtgc
      301 tcatatttat gatcatcata ctcctagtcc tggccaacta cctgcactac aagaagaaag
      361 taactgttgg aggaggatac ctatgatggc aactacgagg ttctggagaa acaaaggtgg
      421 gggattgtag attgcacaga atcatacctc ctaaaatagc ccagaagata atggacctaa
      481 tattctgggc ttcagggatc atcaccctgc tgattttgct gatggtcatt ggttacgtgt
      541 tttacaaggg cctgccagta attaacttgg aatttctatt cacagatccc atggactctg
      601 gtaaggctgg tggaatattt cccatgattg tttccagttt atatgtgact ttcatcgcag
      661 ttttagtgtc agccccgata ggagtgggtg cagcattata tttagcagag tatgcaggag
      721 aaaataggac ggtcaggttg atccgcttcg gagccgaaac cctgtcatcc attccttcca
      781 taatatttgg gttgtttggt ttagcattct tcgtgatcta tcttcgaatg ggatggagcg
      841 ttctatctgg aggattaaca ttggcactga tggctttacc caccattctg gcggcctcgg
      901 aggtttccat agaatccatc aataaatcct atgcccaggg tagcctggca ctgggagcaa
      961 ccaagtggca gacagtatct aaagtggtta taccagccgc gttaccagga ataacaactg
     1021 gaatcattct gggaatggca agggccatag ccgaagccgc tgccatattg tataccgtgg
     1081 gtgcagcact aataactccc atatccctgc ttgatccggc acgaccctta ccactgcacc
     1141 tctacatcct ggccaccgag gggatatccc tggataatgc ctggggcacc gcagctgtgt
     1201 tggtgatttt gatcctcata attaccgtga ccaccaatac cctggtggat cgttaccgaa
     1261 aacagatgat ggggcgataa gaaaatggaa tatataatag aagcagaaaa tctcaacgtt
     1321 tacttcgatg aattacacat cctaaaaggc g
//
LOCUS       DAOQVW010000491         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_139118_length_1348_cov_2.650425, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000491 DAOQVW010000000
VERSION     DAOQVW010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139118_length_1348_cov_2.650425"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="PKK85_08910"
     CDS             complement(<1..1294)
                     /locus_tag="PKK85_08910"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS26172.1"
                     /translation="MINKKRKNQLRSPFNRHLKGNYSHEPCGEELIHSREIFSDIIKH
                     LPDATFVINSDGAVVAWNQAMEELTDVKAEYIMGKDDYEYSIPFYGERRPILIDQVFK
                     LDSQLARTYQKFKSKEEAITAEVFIPKLRGKSTYLWGKAAPLYNRRREIVGAIESIRD
                     ISDMKQAEVEINKYQDHLEELVEKRTSELRNSEKKLKDIIYGSPIPTFIIDRNHRVIY
                     WNQALEEFTEIMAEEIIGTKDHYRIFYSNKRPCLADLLVEDGIEDIEYWYSGNYQRSK
                     LIEGAYHVTDFFPLLGKDGKWLTSTTAVIRDAKDDIIGAMTVIEDITQRRLVEEALQK
                     SESKYRTIFENTGTATVLIQEDTTLSLVNSEFVRLTGFSREEAHGKSWLEFVSEDDKD
                     RMIKYHLLRTMDSKIPPQNYEFSFLRKNGTFGDGYMTIE"
BASE COUNT          368 a          286 c          228 g          466 t
ORIGIN      
        1 tctcgatggt catgtaacca tccccaaaag ttccattttt tcttaaaaaa ctgaattcat
       61 agttttgagg cggtattttt gagtccatgg ttcttaacag atgatatttg atcatcctat
      121 ccttatcatc ttcagaaacg aactcaagcc aacttttacc atgggcttct tcccgtgaga
      181 aaccggttaa cctaacgaac tcagagttta ctaaggatag ggtggtatct tcttgaatga
      241 ggacggtggc agtgcccgta ttttcgaata tggtcctata tttagattca gatttttgta
      301 gagcttcttc tactaatcga cgctgtgtta tgtcctcaat aaccgtcata gctccgataa
      361 tatcgtcttt agcatccctg atcactgcgg tggtggaggt aagccattta ccgtccttcc
      421 ccaaaagtgg gaaaaagtca gttacatgat aagctccttc tatcaatttg gatctttgat
      481 aatttccaga ataccagtat tcgatatctt ctatcccatc ttccacaagc agatccgcta
      541 aacagggcct tttgttactg taaaatatac ggtaatgatc tttggttcca attatttcct
      601 cggccataat ttcagtgaat tcttccagtg cttgattcca gtagattact cgatggtttc
      661 tatctatgat gaaggtggga atcggtgaac cgtaaattat gtcttttagc tttttttcgc
      721 tgtttcttag ctctgaagta cgtttttcaa ccagttcctc cagatgatcc tgatatttat
      781 ttatctccac ttcagcttgt ttcatatcag agatatccct tatggattca atagcaccaa
      841 ctatctctct ccgtctatta tatagtggag cagcctttcc ccacaaatag gtcgacttac
      901 ccctaagttt gggaatgaac acttcggctg tgatggcctc ttctttactt ttgaatttct
      961 gataggtacg tgccaactgt gaatctaact taaacacctg atcaatcagg atgggccttc
     1021 tttcaccata aaatggaatt gagtattcat agtcatcttt acccatgata tattcagctt
     1081 taacatctgt taactcctcc atggcctgat tccaagcaac aacagctcca tcagaattta
     1141 ttacaaaggt agcatcaggg agatgtttaa tgatatccga gaaaatctct cgggaatgga
     1201 ttaattcttc accacaaggt tcatgactat aatttccttt caaatgacga ttaaatggtg
     1261 atctaagctg attcttcctt tttttattga tcatgaaatt ccaacattat ctttaaaatt
     1321 tgattgcaaa caaatcttaa taatatct
//
LOCUS       DAOQVW010000492         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_139261_length_1347_cov_2.888545, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000492 DAOQVW010000000
VERSION     DAOQVW010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139261_length_1347_cov_2.888545"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKK85_08915"
     CDS             complement(<1..678)
                     /locus_tag="PKK85_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNS26173.1"
                     /translation="MLEILEYSFMQNAFLAAILVSVACGVVGCYVVIKRIVFISGGIS
                     HSAFGGVGLGYFLGVNPLLAAVPFTLLAAAGMGLVKKRMEVPEDTAIGILWSVGMALG
                     IIFVNLTPGYAPDLISYLFGNILTVPTTDLYLMLLLDAVILVMVYLLRREFLALSFDE
                     EFSTVVGMPSEAVYYLLLALVALSVVVLIKVVGVILIIALLTIPAAITREYTNNLNQM
                     MVLSVVLG"
     gene            complement(765..>1347)
                     /locus_tag="PKK85_08920"
     CDS             complement(765..>1347)
                     /locus_tag="PKK85_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNS26174.1"
                     /translation="SFLAIIGPNGGGKSTLLRMILGLLTPEEGKVEVFGRDPRKAREY
                     MGYLPQHVSFLHDFPINVEDTVLTGRYHGLFSNYTPGDREAVHQTLEMVDLDGLADRQ
                     MGQLSGGQVQRVFLARALVRRPKLLLLDEPMASVDPQMQYSFYQLLEELKEDEHHPGH
                     PRCGGGIRPGGRYRLPQPAPLLPWPGRGCRGGY"
BASE COUNT          322 a          403 c          347 g          275 t
ORIGIN      
        1 tcccaacacc actgatagaa ccatcatctg gttcaggtta ttggtgtact cccgtgttat
       61 ggctgcgggt atggttaaaa gggcgatgat gagtatcacc cccaccacct taatgagcac
      121 caccactgac agggccacca gggccaaaag caggtaataa acagcctccg atggcatgcc
      181 caccacggta ctgaactcct cgtcaaagga taaagccagg aactctctgc gcaggaggta
      241 caccatcacc aggatcaccg cgtccagaag gagcatcagg tacaggtcag tggtggggac
      301 ggtgaggatg ttcccaaaga ggtagcttat caaatccggg gcatatcccg gggtgaggtt
      361 gacgaagatg atacccaggg ccatgcccac actccacagt ataccaatgg cagtgtcctc
      421 cggtacctcc atcctcttct ttacaaggcc catgccagcg gctgccagga gggtgaaggg
      481 caccgccgcc aggagtggat tcacacccag gaagtagccc aggcccactc caccaaaggc
      541 ggagtggctg attcctccac ttatgaacac gatcctcttg atgaccacgt agcaccccac
      601 cacaccacag gctacgctga ccaggatggc cgccagaaag gcgttctgca tgaaactgta
      661 ctccaatatt tccagcataa ctattcaact actcgtgtct tttcagcatc cgatggggag
      721 tggcgccatg ggtgattatc tccaggggac atccgtacat cttctcaata acctcccctg
      781 caccctctac ctggccatgg tagtagaggc gctggttgag gcaggcgata tcttccacct
      841 ggtcgaatac cgcccccaca tcgtgggtga ccaggatgat gctcatcttc tttcaactcc
      901 tccaaaagct ggtaaaagga gtactgcatc tgggggtcca cactggccat gggctcatcc
      961 aggagtagga gtttaggcct tctaaccagg gcccgggcca ggaaaacccg ctgcacctgt
     1021 ccccctgaca gctgtcccat ctgacgatct gccaggccat ccaaatccac catctccagg
     1081 gtctgatgaa ctgcctcccg gtcccccggg gtgtagttgg aaaagagtcc atggtaccgg
     1141 ccagttaaaa cagtgtcctc tacatttatg gggaaatcat ggagaaagga cacgtgctgg
     1201 ggcagatacc ccatatattc acgggccttc ctggggtccc ggccaaagac ctccacctta
     1261 ccctcctccg gggtgaggag gcccagtatc atgcgcagca gggtgctttt tcctccacca
     1321 ttggggccga ttatggccag gaatgat
//
LOCUS       DAOQVW010000493         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_139367_length_1347_cov_1.736842, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000493 DAOQVW010000000
VERSION     DAOQVW010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139367_length_1347_cov_1.736842"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PKK85_08925"
     CDS             complement(<1..690)
                     /locus_tag="PKK85_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS26175.1"
                     /translation="MVRDTTVLMDEKRVFEPSEEVKNRAHVKDWDAEIEKGKDLGKYW
                     AQQAEQFDWIKKWDKVLDDSNKPFYKWFTGGKINLAYNAVDRWIETEKRNQIAIIYIN
                     ERGHEKKITYYELYLEVNKLANALKNLGVKKGDTVSMYLPMCPELMIALLACNKIGAV
                     HSVVYSGLSVGAFVDRMNDVLRLNRGNAVLDRLPYCLDANLILGQIWTSGGREEEADV
                     HLKRAQALDPQS"
     gene            complement(894..>1347)
                     /locus_tag="PKK85_08930"
     CDS             complement(894..>1347)
                     /locus_tag="PKK85_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNS26176.1"
                     /translation="VASTVEDGKVVSGGGAPEIAISKALREYADTVSGREQLAITAFA
                     DALEVVPKTLAENAGLDQIDALADLRAAQEHSHHMGLDVFQGEVTDMNEGNVIEPHRV
                     KKQAIQSAAEAAEMILRIDDMIASAGVTEPEGMEGMPPGGMGGMPPMM"
BASE COUNT          304 a          343 c          299 g          401 t
ORIGIN      
        1 cgattgcggg tcgagcgcct gggcgcgctt gagatgcaca tcggcctctt cttcgcgccc
       61 gccgctggtc cagatttgac cgaggatgag attcgcgtcc aggcagtagg gcagccggtc
      121 gagcaccgcg ttgccgcggt tgagacgcaa aacatcgttc atccgatcca cgaaggcccc
      181 caccgagagt ccggagtaaa caacgctgtg cacggcgccg atcttgttac aggccagaag
      241 ggctatcatt agttcgggac acatgggcag gtacatggaa accgtgtccc ccttctttac
      301 tcccaggttt ttcaaggcat tggccagttt attaacttcc aggtagagtt catagtaggt
      361 gatcttcttt tcatggccac gttcattgat gtagatgata gcgatctggt tacgtttctc
      421 ggtttcgatc cagcgatcca cggcattgta ggccaggtta atcttacccc cggtgaacca
      481 cttgtaaaat ggtttgtttg agtcatcaag gactttatcc cattttttta tccaatcgaa
      541 ttgttctgcc tgttgggccc agtattttcc caggtctttg cctttctcta tttcggcatc
      601 ccagtctttc acatgggctc ggtttttcac ttcttcacta ggttcaaata ctcttttttc
      661 gtccataagg actgtagtat ctcgtaccat tcatttccca ccattttcct gttttagcta
      721 atgattctaa ctaagttact ctttctatat atattttact atgattaatc attataaatt
      781 catattaatc atgataaata tactggtgtg tagagaagaa gtgctggtgt tatcctgtaa
      841 aaaatagtgg tatgcaatta aaaataaaaa aaagaaggat aattatcctt caactacatc
      901 atagggggca ttccacccat tccaccagga ggcattcctt ccatgccttc tggttctgtg
      961 acgccagcag aggctatcat gtcgtcaatg cgcaggatca tctcagcagc ctcggctgca
     1021 gattgaatag cctgtttttt aaccctatga ggttctataa cgttaccctc attcatatca
     1081 gtgacttctc cttggaagac gtccagtccc atgtggtggc tgtgttcctg ggctgcccgc
     1141 agatccgcaa gagcatcgat ctgatccagt cccgcgttct cagccagggt cttgggaacc
     1201 acttccaggg catcggcaaa ggcagtgatg gctaactgtt ccctgccact gactgtgtca
     1261 gcgtattccc gtaatgcctt tgatatagct atttcagggg ctcctcctcc agaaactacc
     1321 tttccatctt ccacggtgga tgcaacc
//
LOCUS       DAOQVW010000494         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_140085_length_1342_cov_4.140637, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000494 DAOQVW010000000
VERSION     DAOQVW010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140085_length_1342_cov_4.140637"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /gene="msrA"
                     /locus_tag="PKK85_08935"
     CDS             <1..489
                     /gene="msrA"
                     /locus_tag="PKK85_08935"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNS26177.1"
                     /translation="LEAIYKRVKGVLEVVSGYSGGNIKNPTYEQVCSDRTGHAEAIQI
                     IFNPEIISYDEILKIFWKIHNPTTHNRQGNDVGSQYRSMIFYHNEKQKEIAESSKKDL
                     QDAHIWHDSIVTEIVPFIEFYPAEEYHQDYYENNPHNSYCQFVVGPKIKKFVKECKEY
                     LK"
     gene            513..1247
                     /locus_tag="PKK85_08940"
     CDS             513..1247
                     /locus_tag="PKK85_08940"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNS26178.1"
                     /translation="MVKEKNFQSNNYTAAFLIMGISIVLAALVFGFFFYQTRSSRDYV
                     QVLGAATQGFESDIVKWNLTLEESTDLHNVIDGYRNIQLKRKKLIDILVNKGISNDDI
                     NINPISSYNRWGREGEMTGYTLNQSLFIISTEIEAIETLALNPDELLDQEIFFQTSSL
                     EYFYSKIDDMKKDLLELATRNARERADMILKESNHKAGKMIWSQAGVFQIIEPYSTEV
                     ESYGMYNTASRKKEIKVTVHANFLIE"
BASE COUNT          478 a          208 c          254 g          402 t
ORIGIN      
        1 ctggaggcaa tttataaaag agtaaagggt gttctggaag ttgtttccgg ttatagtggt
       61 ggtaatataa aaaatccaac ctatgaacag gtctgttctg atagaaccgg ccatgctgag
      121 gcaattcaaa taatttttaa tcctgaaatt atttcctatg atgagatatt aaaaatattc
      181 tggaaaattc acaatcccac cacccataat cgacagggca atgatgtagg gtctcaatat
      241 cgttcaatga ttttttatca taatgaaaaa cagaaagaaa ttgctgaaag ttcaaaaaaa
      301 gatttacagg atgcccatat atggcacgat tctattgtaa cagaaatagt tccttttata
      361 gaattctatc cggcagaaga atatcaccag gattattatg agaataatcc ccataatagc
      421 tattgtcagt ttgtggtagg acccaagata aaaaaatttg tcaaagaatg taaagaatac
      481 cttaaatagt aacaggaaaa aggaggagta aagtggtaaa ggagaaaaat tttcaatcta
      541 ataattatac agctgcattc ctgataatgg gaatatctat tgttttagcc gcactagtat
      601 ttggattctt cttttatcaa accagaagca gccgtgatta tgttcaggta ttaggtgctg
      661 cgacgcaagg atttgaatca gatattgtga aatggaattt aactctggag gaaagcactg
      721 atttgcataa tgttatagat ggttaccgaa atattcagct aaagagaaaa aaattaatcg
      781 atatattggt aaataaagga atatcaaatg atgatataaa cattaacccc atttctagtt
      841 ataaccgatg gggccgggag ggggagatga cgggttacac cttaaatcag tcacttttta
      901 taatctctac agaaattgag gctattgaga ctttagccct aaatccggat gaacttttag
      961 atcaggaaat ctttttccag acttcatctt tagaatattt ttactccaaa attgatgata
     1021 tgaaaaaaga tttattggaa ttagccacca gaaatgcacg ggaaagggca gatatgattt
     1081 taaaggagag caatcataaa gccgggaaaa tgatctggtc ccaggcaggt gttttccaga
     1141 ttattgaacc atattctact gaggtagaaa gttatggaat gtacaacact gcatcccgga
     1201 aaaaggagat taaagtgact gttcacgcca actttttaat cgaataaaaa ccccaccact
     1261 tcttatgcct tattccatat taagcgtatc agcaatacat ctgtttttct cattcatgat
     1321 atttaatatt taattctgaa ct
//
LOCUS       DAOQVW010000495         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_141087_length_1337_cov_2.121685, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000495 DAOQVW010000000
VERSION     DAOQVW010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141087_length_1337_cov_2.121685"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1337)
                     /locus_tag="PKK85_08945"
     CDS             complement(<1..>1337)
                     /locus_tag="PKK85_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNS26179.1"
                     /translation="PEKTYGKRKKPHLPPVMKSLLITLSTTLLLSALTQPALAAMWYP
                     SSPYGSPKTSSSLWNPPGTSSSPRDSLGTIFSSHDSLGKTPLEVLTADEAVRFALEHH
                     PGVQAAALKVRQASLMVPAGFNPEKTALFYNYDQNNIAENGLPIKVWGLRQNFSFPTV
                     YTSRTKLLKTVTGVEEARYQLVVRETERNVRQKYDEVLILSRKVTLLREIDSLYQATA
                     TRSDLKFRASYGNVGNQSIGNYLYYSGMKTRPTSNWADVSGVRYLTLDAPGLISGDFT
                     WEKVTTRNIGVDLGLLSNRFTGSFDLFKRTTTGMLIPGAQLPAVLGASTPLQNTADLK
                     STGWGLQAAWKDQIGKVNYQFGFTLSDNKAFITKYDINATHLLTQYYVGQQIGEIWGY
                     ETDGFYTVGDFEEGSLNSNLMGGKLLDGVIKYQGQNPNPGDIKFKDLDKDGKI"
BASE COUNT          293 a          307 c          369 g          368 t
ORIGIN      
        1 aatattttcc cgtctttatc aaggtcttta aatttaatat ctcccgggtt aggattttgc
       61 ccctggtact taattactcc gtcaagcagt tttcctccca tgagattgct gtttaaacta
      121 ccttcttcaa aatccccaac ggtatagaat ccgtctgttt cataccccca tatttcccct
      181 atttgctgcc caacataata ctgcgtaagg aggtgagttg cattaatatc atatttggta
      241 ataaaggcct tgttatcgga aagcgtaaat ccgaattgat aattcacttt acctatctgg
      301 tctttccatg ccgcctgtaa cccccatccg gtagatttca gatcggccgt attttgaagg
      361 ggtgtgctgg cgcccaatac agcgggcagt tgtgcaccgg gaatcagcat gcctgttgtg
      421 gtacgcttaa acagatcgaa gctacccgta aacctgttgc tgagcagtcc gagatcaacc
      481 ccaatattcc tggtagttac tttttcccat gtaaagtctc cactgataag cccaggggca
      541 tctaatgtaa gataccttac ccctgaaaca tctgcccagt tggaagtagg ccgggttttc
      601 attccggaat aatacaggta attgcctata gactggttgc ccacgttacc atagcttgcc
      661 ctgaatttca gatcagacct ggtggcagta gcctgataga ggctgtcgat ttcacgaagg
      721 agcgtcactt tcctggaaag gatgagtacc tcgtcgtatt tctggcggac gtttctttcg
      781 gtttccctga ccaccagttg gtagcgggct tcctcgacgc ctgtcaccgt tttgaggagt
      841 ttagtccggg aggtgtacac ggtggggaag ctgaagtttt ggcggaggcc ccatactttt
      901 ataggcaggc cgttttcggc gatgttgttt tggtcatagt tgtagaacag ggccgttttc
      961 tcaggattga atcctgcggg gaccatcagg gaggcctggc ggactttcag ggcggcggcc
     1021 tgcacgccgg ggtggtgttc cagggcgaag cgcaccgcct cgtcggcggt cagcacttct
     1081 agtggggtct tgcccaggga gtcgtgggat gaaaagatgg ttcccaggga gtcacggggt
     1141 gaagaagagg ttccaggggg attccagagt gaagaagagg ttttcggaga tccataggga
     1201 gaagaggggt accacatggc tgccagtgcc ggctgggtta aggcagacag cagcaaggtt
     1261 gtggacagtg tgatcagcag ggatttcatc actggaggaa ggtgtggttt cttgcgtttg
     1321 ccgtaggttt tttcggg
//
LOCUS       DAOQVW010000496         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_141264_length_1336_cov_2.035129, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000496 DAOQVW010000000
VERSION     DAOQVW010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141264_length_1336_cov_2.035129"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKK85_08950"
     CDS             <1..234
                     /locus_tag="PKK85_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNS26180.1"
                     /translation="GTTVFVLIFNSAAGIGGYFLSGRLDVPLVILLGGGAVIGAFIGP
                     KLIKKVGPKTVEKIYAPLLIGISLVLGLSLILA"
     gene            complement(279..>1336)
                     /gene="dnaJ"
                     /locus_tag="PKK85_08955"
     CDS             complement(279..>1336)
                     /gene="dnaJ"
                     /locus_tag="PKK85_08955"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNS26181.1"
                     /translation="DVSDDPESAEKFKELSEAYAVLSDEEKRNTYDQYGHAGMGGFSQ
                     EDIFNNINFEDIFKGFGFGGSSGGGAGFESIFDLFGFGGGRRNGPQQGDDLAYHLDIT
                     LEEAAQGLERDIEVAHKKPCPKCHGSKAEPGTEPRVCPTCGGSGQVRQVSNTPLGQFA
                     TIRPCNTCQGEGKIIDTPCSECNGKGIVKQNSTIHIKIPPGVDDGSRLRVPGEGDAGK
                     RGGPPGDLYILIKVKKHPLFKRDGSDLIYEMPISFVQASLGDTVEAPTLDGAVDLKIP
                     SGTQSGTSFRIKGQGMPHMRWTGKGNLYVKVKVITPKKLNSKQKELLREFADASGEEI
                     YAEDKGFFDKVKDAMGH"
BASE COUNT          290 a          336 c          313 g          397 t
ORIGIN      
        1 ggcaccacgg tatttgtact catctttaac tcagccgcag gtattggagg ttacttctta
       61 tcaggcaggc tggacgtgcc tctggtaatt ctccttggtg gcggagcagt tataggtgca
      121 tttatagggc ctaaactcat aaaaaaagta ggtcccaaga ctgttgagaa gatatatgcc
      181 ccactactca ttgggataag tcttgtactg ggactgtcac taatactggc ttaaaaaaag
      241 tttggaataa attaaggata ggaaacaagt gggaaatttc aatgtcccat agcatccttc
      301 actttatcaa agaatccttt atcttcggca tagatctcct cgccactggc atcggcaaat
      361 tcccggagca actccttctg tttggagttc aacttcttgg gagttataac ttttaccttg
      421 acgtacagat tacccttacc ggtccaacgc atatggggca tgccctggcc ttttatccgg
      481 aaggaagtgc cgctctgtgt tccagaaggt attttaagat ccactgcacc gtcaagggtt
      541 ggagcctcta cagtgtcgcc cagggaagcc tggacaaagc ttataggcat ctcgtagatt
      601 agatctgagc cgtccctctt aaatagggga tgttttttca cctttatcag gatgtaaaga
      661 tcacctggtg gacctcccct tttaccggca tcaccttcac cagggacgcg caggcggctg
      721 ccgtcatcca cgcctggagg tatcttgatg tgtatggtgc tattctgttt aacgataccc
      781 tttccattac attcgctgca gggggtgtcg attatttttc cctctccctg acaggtgtta
      841 cagggtctta tggtggcaaa ctggcccagt ggagtgttac ttacttggcg gacctgtcca
      901 cttccaccgc acgttggaca gacgcgtggt tctgtccctg gttctgcctt agaaccatga
      961 catttagggc agggtttttt atgggctact tcgatatccc gttcgagtcc ctgtgctgct
     1021 tcttccaggg tgatgtctag gtggtaggcc aggtcatctc cttgctgggg tccgtttctc
     1081 cttccgcctc caaatccgaa gaggtcgaat atgctttcaa atccagctcc tccaccagaa
     1141 cttcctccga atccaaatcc tttgaagatg tcttcgaagt ttatgttgtt gaatatgtct
     1201 tcctgggaga atccgcccat cccggcatgt ccgtattggt cataagtgtt tctcttctct
     1261 tcatcggaaa gcacggcgta agcttcgctg agttctttga acttctccgc actctctggg
     1321 tcgtcactca catcgg
//
LOCUS       DAOQVW010000497         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_141503_length_1334_cov_3.767787, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000497 DAOQVW010000000
VERSION     DAOQVW010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141503_length_1334_cov_3.767787"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKK85_08960"
     CDS             <1..273
                     /locus_tag="PKK85_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNS26182.1"
                     /translation="LDFEFAGNHTNLSLLENVDLGGKKIGFGCIDTKTVKVESVEEIH
                     KLINKGMDIVGKEKMIIDPDCGMRMLNQESAIQKLKNMTEAVKWLS"
     gene            261..878
                     /locus_tag="PKK85_08965"
     CDS             261..878
                     /locus_tag="PKK85_08965"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNS26183.1"
                     /translation="MAILIRAPTIKSGWESLVKRIINNGLEINDERGSLTLEILNTVV
                     SVQKPLQLDIPDGYFWRGEKLEKYAQQFLSDDPQGFIYTYGNRLRKHFQGIDQIREAI
                     NRLKNCKESRRAISITWDPPTDTKNDEVPCMIIVDFKIRNGKLQTTGLWRSHDIYGAW
                     FPNAVGLSYLASYVAKEVGVDVGTLTIHSISAHIYEVNFDEARRV"
     gene            1011..>1334
                     /locus_tag="PKK85_08970"
     CDS             1011..>1334
                     /locus_tag="PKK85_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HNS26184.1"
                     /translation="MLSVNLEAKKTVDLMIERAEELNLAVAKLENDSTIIDAGVNVTG
                     SFKAGEMYTKVCLGGLAEVGISIPGDLSKTLALPAVKIKTDFPSISTLGSQKAGWSVS
                     VGDFFA"
BASE COUNT          443 a          236 c          310 g          345 t
ORIGIN      
        1 ttggactttg aatttgcggg taaccatacc aatctatctc tactggaaaa tgttgacttg
       61 ggcgggaaga aaattggatt tggttgtatc gataccaaga cagtaaaagt ggagagtgtg
      121 gaagaaattc ataaactcat taacaagggt atggatattg ttgggaaaga aaaaatgatc
      181 atcgaccctg attgtggtat gagaatgctc aaccaggagt ctgcaataca aaaactgaaa
      241 aacatgacgg aggcagtaaa atggctatcc taataagagc ccccaccata aaatcgggtt
      301 gggaaagtct ggttaagaga attataaata atggattaga aatcaatgat gaaaggggat
      361 ctttaacgtt ggaaatctta aatactgttg ttagtgtcca aaaacccctt cagcttgata
      421 taccggacgg ttacttctgg cgaggagaaa agctggaaaa gtatgcccag caatttttat
      481 ccgatgatcc acagggattc atatacactt atggcaatcg acttcgaaaa cactttcagg
      541 gcatagatca gatacgggaa gctataaatc gcctaaaaaa ctgtaaagaa tcgcgtagag
      601 ccatatctat aacatgggat cctcccactg acactaaaaa tgatgaagta ccctgtatga
      661 tcatcgttga ctttaaaatc aggaatggaa aactccaaac tactggtttg tggcgatccc
      721 atgatatata cggggcctgg ttccccaacg ctgtaggatt atcatatctg gccagttacg
      781 ttgccaaaga agtaggagtg gatgtgggaa cccttaccat ccattccata agtgcccaca
      841 tctacgaagt caactttgat gaggcaagaa gggtgtaaag ggttcgaatc tttaaaacag
      901 ccgagagttg tgaatttaga tttctttgga tgcaatgaaa aaataaaaaa gcttaaaaat
      961 tatctgggaa taagagaata agtgattata actttcattg aggtgaacga atgttaagtg
     1021 ttaacctgga agctaaaaaa actgtggatt taatgattga acgggccgaa gaactcaacc
     1081 tggcggtagc taaattggag aatgattcaa ccataataga tgctggagta aatgttacgg
     1141 gaagtttcaa agcgggtgaa atgtacacca aggtctgtct gggcggtctg gccgaagtgg
     1201 gcatatccat cccaggtgac ctttcaaaaa cattggccct tcctgctgtg aagattaaaa
     1261 ctgacttccc atccatctca actttgggat cccagaaagc aggctggtca gttagtgtag
     1321 gtgacttttt cgcc
//
LOCUS       DAOQVW010000498         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_142394_length_1329_cov_3.270801, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000498 DAOQVW010000000
VERSION     DAOQVW010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142394_length_1329_cov_3.270801"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /gene="rplP0"
                     /locus_tag="PKK85_08975"
     CDS             <1..491
                     /gene="rplP0"
                     /locus_tag="PKK85_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295095.1"
                     /note="similar to bacterial 50S ribosomal protein L10;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNS26185.1"
                     /translation="RLGIYPMEVGIDLQAAYEDDTVYTSDLLTIDLDKTIQDIQRVYS
                     QAINLAVNAVVFTEESLPLLISKAAGQSMNLALNADILTTKTTDIILAKAYAQMLALA
                     SELSAKDGDALDSELQKKISAKAAPTTVSQDKEEEEEIEEEEEEEEKEEDAAAGLGAL
                     FG"
     gene            526..834
                     /gene="rpl12p"
                     /locus_tag="PKK85_08980"
     CDS             526..834
                     /gene="rpl12p"
                     /locus_tag="PKK85_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HNS26186.1"
                     /translation="MEYIYAAMLLHSANQDVNEDNVKKVLEAAGSEVDEARVKALIAA
                     LEDVDIEEAMEKTAVSAAAPAAAAAAPAEAEETEVEEEEEEEEEKEEEAAAGLGALFG
                     "
     gene            988..>1329
                     /locus_tag="PKK85_08985"
     CDS             988..>1329
                     /locus_tag="PKK85_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNS26187.1"
                     /translation="MIIMSQQLKELGYTKKTCKTCGNYFWSIGNRETCGDAPCDEYSF
                     IGDPATSQKYDLFSIQDIFTRFFKDRGHTPIRRYPVLAKRWRDDVFLVDASIYNFQPW
                     VTSGMVEPPANP"
BASE COUNT          406 a          287 c          303 g          333 t
ORIGIN      
        1 cccgactggg aatctatccc atggaagtgg gaattgacct acaagccgct tatgaggacg
       61 atactgttta tacctctgat cttctgacca ttgacttgga taagaccatc caagatatac
      121 aaagggttta ctctcaggcc ataaatctag ccgtgaatgc ggtggtgttc accgaagagt
      181 ccctgcctct gttaatatct aaagctgccg gccagtccat gaacctggct ctgaatgcgg
      241 atatattaac taccaaaacc actgatatta tcctggccaa agcctacgcc cagatgctgg
      301 ccttggcatc cgagttatct gccaaggatg gtgatgctct ggacagcgaa cttcaaaaga
      361 agataagcgc aaaggccgcg cccaccacag tatcccagga taaagaagag gaagaagaaa
      421 tagaggaaga agaagaggaa gaagaaaaag aagaggatgc agccgctgga ctgggagccc
      481 tcttcggata attattaaca aaatttagaa attcgaggtg atttcatgga atacatatac
      541 gcagcaatgt tactacactc tgcaaatcag gacgtaaacg aagataatgt gaagaaagtt
      601 ttagaagcag ccgggtccga agtggacgaa gccagggtta aagccctcat cgccgcatta
      661 gaggatgtag acatagaaga agctatggaa aaaaccgcag tgtcggctgc tgcacctgca
      721 gctgctgcag ctgccccagc tgaagctgaa gagactgaag ttgaagaaga agaggaagaa
      781 gaagaggaaa aagaagaaga agctgctgcc ggcctcggtg ctctcttcgg atagattctc
      841 ttcctaagtc aaggcgcttc tactttgact tttttttact tcttttttct tttaaattca
      901 ttcccccttc tgatcaaaag ataaatactt agttaatgct gatcaaatcc agggatataa
      961 ttgtaagttt ttttaaaaat ttcatcgatg attatcatgt cccaacagtt gaaagaactt
     1021 ggatacacca agaaaacttg taaaacttgt ggtaactatt tttggtctat tggaaatcgt
     1081 gaaacgtgtg gagacgcccc ctgtgatgag tactcattta ttggggatcc tgctaccagt
     1141 caaaaatatg atcttttctc gattcaagat attttcacgc gtttttttaa agacagagga
     1201 cacactccca tccgacgtta cccagtgctg gccaagagat ggcgtgacga tgttttccta
     1261 gtggatgcat ccatctacaa cttccaaccc tgggtaacct ctggaatggt ggaacctccc
     1321 gccaatcca
//
LOCUS       DAOQVW010000499         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_142671_length_1328_cov_2.093480, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000499 DAOQVW010000000
VERSION     DAOQVW010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142671_length_1328_cov_2.093480"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PKK85_08990"
     CDS             <1..1261
                     /locus_tag="PKK85_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNS26188.1"
                     /translation="LSKRFNSRFIQWFSGAVIFFGMPLYAAVVLIGMARFVETTLNLD
                     YNIALIGMAVIVALYVIFGGIRGVMYTDALQGSIMFVVMIVLLVAIYWITGGLVDGNQ
                     ALTNLVNVVPPDLTATATATGFTGWTSMPTFGSPFWWTLVSTLILGVGIGVLSQPQLG
                     VRFMMVKSNRELNRAVLIGGIFIFLMTGTAFIVGALSNVYFFNTMGQIATQVVGGNAD
                     KIIPAFIAAAMPPWFAYLFMITLLSAAMSTLSALFHVQGTALGRDVYETATKKKGGAS
                     VMVARAGITVAVVIAVILGYLLPANIIAVGTAMWFSITAAAFLSMYLFACFWKRATKE
                     GVISGLVIGTAISIFWIVFVFKKSAEALGICKALTGQAVLIAAQPWPTVDSIVIALPI
                     AFIITVIISLLTKPPEKEHLDKCFSGV"
BASE COUNT          344 a          299 c          303 g          382 t
ORIGIN      
        1 tttatcgaag cgtttcaaca gtagatttat ccaatggttc agtggtgcgg tgatattctt
       61 tggaatgccc ttatatgccg ctgtggtttt aattggtatg gctcgttttg tggagacaac
      121 acttaatctt gattataata tagccttaat aggtatggca gtaattgtag cgttatacgt
      181 tatttttggt ggtatacgtg gtgtgatgta caccgacgcc ctccagggtt ccataatgtt
      241 tgtagttatg atcgtcttac tggtagcgat atactggatt acaggaggcc tcgtagatgg
      301 taaccaggcg ttgaccaacc tggttaacgt ggttccgcct gatttaacag caacggccac
      361 agcgacagga ttcacgggat ggacatccat gcccaccttt ggcagtccct tctggtggac
      421 cctggtcagt accctgattt taggtgtggg tataggtgtt ctctcccaac cacaactggg
      481 agtacgattc atgatggtga aatcaaacag agaattaaac agagcagtat taattggggg
      541 cattttcatc ttcctcatga ctggtaccgc cttcatagta ggggctcttt ccaatgttta
      601 cttcttcaac accatgggac agatagcaac ccaggtggtg ggaggaaatg ccgataaaat
      661 tatccccgca ttcatagcag cagcaatgcc tccctggttc gcgtatctct tcatgatcac
      721 cctgctttca gcggccatgt ccacgttaag cgccctattc cacgtgcagg gaacagcact
      781 gggacgtgac gtttacgaga cagccacaaa aaagaaggga ggagcatcag taatggtggc
      841 ccgggccgga ataacagtgg ccgtggtaat agccgttatc ctcggttacc tcttaccagc
      901 gaacatcata gcagtgggaa cagccatgtg gttctctata accgcagcag ccttcctatc
      961 catgtatctt ttcgcctgtt tctggaagcg tgccaccaag gaaggagtta tctctggact
     1021 ggtaatcggt acagccatca gcatattctg gatcgtgttt gtatttaaaa aatcagcaga
     1081 agcactgggt atctgtaagg cactcactgg tcaggcagtt ttaatagctg cacaaccatg
     1141 gcctacagtt gactccatag ttatcgcact acccatagcg tttatcataa ctgttataat
     1201 cagcctgtta accaaaccac cagaaaaaga acacttagat aaatgtttca gcggagttta
     1261 acaactccct gaatatttta ctttttttac gaacttttct ttttaaatca tccaataaac
     1321 tgtaattt
//
LOCUS       DAOQVW010000500         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_143916_length_1321_cov_2.500000, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000500 DAOQVW010000000
VERSION     DAOQVW010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143916_length_1321_cov_2.500000"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..640)
                     /locus_tag="PKK85_08995"
     CDS             complement(236..640)
                     /locus_tag="PKK85_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26189.1"
                     /translation="MNYKGVILILVVVIIFLVGAVAYLWISQPAENATLNVTNNTTST
                     DNSQEIQTNQSTAAKISAAKAKSIASDYMASKPEQFSNTKAGTPSLKGGIYYVPVVIT
                     KEGQHSVGTVVAYIKVDGETGSVFGIQEIEMY"
     gene            complement(791..>1321)
                     /locus_tag="PKK85_09000"
     CDS             complement(791..>1321)
                     /locus_tag="PKK85_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatinine amidohydrolase"
                     /protein_id="HNS26190.1"
                     /translation="GAAFLGVLYAATEYNYVKHGVHLKPLELVEKQLKPTLRSAKKNL
                     QLEKIILVNGHGGNMPIRENLKGIEEDLGLHISFINRIVDIEGPHAGSGELSMGEFLG
                     ITDQERLGEHCDVEKYPEFCMIGLEEARNADEGIDRGARESEGKNISADPELGKSLLE
                     EAIRDVVKDIKSLVDD"
BASE COUNT          350 a          277 c          239 g          455 t
ORIGIN      
        1 gatattatat ggtcatttga taacatcccc ttgattttat aggtatttac tacggtataa
       61 tacctgtctg gttgatgtag attaaatggt aattatttaa atggtaatta tgctctgttt
      121 ttagaatagt tccacagccc tggcaaaaat aagttccatg gcactaacta tgcgtacaga
      181 tgaataagtt acaaactcct gactaaatta atgaataaaa aagaaatgag tgagtttagt
      241 acatttcaat ctcctgtatt ccaaaaacac ttccggtttc tccgtccact ttgatgtagg
      301 ccaccacagt acctactgaa tgctggcctt ccttggttat aaccacggga acgtaatata
      361 tacctccttt taaagagggt gtgcctgctt tagtattgga gaattgttcg ggtttggaag
      421 ccatgtaatc tgatgcaatg gatttagcct ttgctgcaga tattttggct gccgtggatt
      481 gattggtctg tatttcttgt gaattatctg tagaagttgt gttgttggta acatttaagg
      541 tagcattctc tgcaggttgg cttatccaca agtaggctac tgctccgact aggaatatta
      601 tcactactac caggatcagg ataacacctt tataattcat agataaatca tctcctacca
      661 tttctattct ttatctctct ttgttaataa ttttttggat aaaatcttat attcagcttt
      721 ttagagaggt aaatacaatc ttcggtcgta aaaaaatttc tagaaaccta gaaggctgag
      781 attgagtttc ctaatcatct accaaacttt ttatatcttt aaccacgtct ctgatagcct
      841 cttccagcag agacttacct agttctgggt cagcactgat gttttttcct tctgattccc
      901 tggctcctct atcaattcct tcatcagcat ttctagcttc ttcaagtcca atcatgcaga
      961 attcaggata cttttccacg tcacaatgtt ctcccagcct ttcctgatcc gttatgccca
     1021 ggaactcacc catggaaagt tccccacttc cagcatgcgg gccttcaata tccacgattc
     1081 tgttgatgaa actgatatgc agtccaagat cctcttcgat accctttaga ttttccctga
     1141 taggcatgtt tccaccatgc ccattgacaa ggatgatttt ctccagttgt aaattttttt
     1201 ttgcagagcg tagggtgggt ttgagttgtt tttctaccag ttccagaggt ttcaagtgca
     1261 cgccgtgttt aacatagttg tattcggtag cagcatataa cacgcctaaa aatgcggccc
     1321 c
//
LOCUS       DAOQVW010000501         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_145119_length_1314_cov_4.004766, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000501 DAOQVW010000000
VERSION     DAOQVW010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145119_length_1314_cov_4.004766"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKK85_09005"
     CDS             complement(<1..751)
                     /locus_tag="PKK85_09005"
                     /inference="COORDINATES: protein motif:HMM:NF016842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMD3-related protein"
                     /protein_id="HNS26191.1"
                     /translation="MFCPQCGREDEELYQGLCRLCYIKETPLINCPDDVEVIICSHCN
                     STFQAGRWTDSDLDPEELMIKILNESIKPAKAAEYVDLSFKIINQRGSKWDVMIHTQG
                     RLLGEEVDQECRVTVKMKRSVCPDCSKYASGYYEAVIQLRADKRQLESGEKEKADSIL
                     KKLLQKLVEKNRMAYLVDRVEIREGVDYYIGSYKAARSVVNGLKDSLGGILGESPRLM
                     GRDKSAGKDLYRIWISLRLPSFKKGDFLAYSD"
     gene            complement(773..1180)
                     /locus_tag="PKK85_09010"
     CDS             complement(773..1180)
                     /locus_tag="PKK85_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HNS26192.1"
                     /translation="MTDYEELLDRAVEQLPQKVLETKRFQVPKAYSMIQGNRTMIQNF
                     GEITDAMNRDPQHLLKFLLRELGTAGNLEGNRAILQGKFTHYLINERIDDYVKRFIMC
                     HECNRPDTRIVREDRVFLLKCEACGAKAPLKTL"
BASE COUNT          336 a          326 c          261 g          391 t
ORIGIN      
        1 aatcactgta ggccagaaaa tctcccttct tgaagctggg cagccgcagc gatatccaga
       61 tccggtataa atccttccca gcggacttgt ccctacccat gagacggggt gactccccca
      121 aaatacctcc cagactatct ttcaatccat taactacact ccgggcggct ttgtaggatc
      181 cgatatagta gtctactcct tctctaattt ccacccgatc cacaaggtag gccatccggt
      241 ttttttccac tagtttctgc agtaactttt tcagaattga atcagctttt tccttttcac
      301 cagattctaa ctgacgttta tctgccctta gttgtattac tgcttcataa tagccggaag
      361 cgtatttact gcagtcagga cacaccgacc ttttcatttt aactgttacc cggcattcct
      421 gatccacttc ctcccccaga aggcgtccct gggtatggat catcacgtcc cacttagatc
      481 ctcgttgatt aattatctta aaagaaaggt ccacatattc ggcggccttt gctggcttga
      541 tggactcatt taagattttt atcatcagtt cttctggatc tagatccgaa tcagtccatc
      601 taccagcctg gaaggtggaa ttacagtggc tgcagatgat gacttccaca tcatctgggc
      661 agtttatcag gggagtttcc tttatgtaac aaaggcggca aagtccctga taaagttctt
      721 catcttctct gccgcattgg ggacaaaaca tgttaattta tcttaaaata gtttaaagtg
      781 ttttcaatgg tgctttagcc ccacaagcct cacattttaa gaggaaaact cgatcttccc
      841 tgactatacg ggtatcgggc cgattacact catggcacat gatgaaacgt ttcacgtagt
      901 catcgatacg ctcattaatg aggtaatggg tgaattttcc ctggagtatg gcgcggttac
      961 cctccaagtt cccagcagta cccaactccc gcagtagaaa cttcaaaaga tgctgaggat
     1021 cccggttcat ggcatctgta atctctccaa aattctggat catggtccgg ttcccctgga
     1081 tcatggaata ggccttgggt acctggaaac gtttggtctc cagcaccttc tggggtagtt
     1141 gttccacggc acggtctaat aattcttcat aatcagtcat taaatacctc ctctaattca
     1201 aaatcaacta aaaatcgcat aagatagctg tattttagta aaatggagaa taaaattcag
     1261 gctaccttgt agtagccggt gcttggttcg aagataagtc cctggcgttt caag
//
LOCUS       DAOQVW010000502         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_146181_length_1308_cov_3.881883, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000502 DAOQVW010000000
VERSION     DAOQVW010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146181_length_1308_cov_3.881883"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKK85_09015"
     CDS             <1..634
                     /locus_tag="PKK85_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNS26193.1"
                     /translation="SGETGCSDTLVREELVRVNPKILGTVYSLMHEKPPLRSIEDYSR
                     ILDACGKSKKHTVGMSICLRDRDEALMKGVELLHRYQDRLLSGLEWIRREGSQEMEYV
                     QYIYTEDKKRKNILGTLASVGLELGFLNPEKPVLTLARLHQDVKVSARTTPQMTAKGV
                     NLGLVMEQASHNFNGSGGGHNIAAGAMIPYQDLENFKNLVNDMVGSQISS"
     gene            660..>1308
                     /locus_tag="PKK85_09020"
     CDS             660..>1308
                     /locus_tag="PKK85_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HNS26194.1"
                     /translation="MYLNPEEEKMYQGDYGPAVEKSMEILVALGDIYQAEKMVEISSA
                     QISGVSYKTIGDAGLEFLEEMAQTARVRVPSTLNPAGVDLDHWEELGFSREFTERQLR
                     IVSAYQKMGITTTCTCTPYLASNLPTLGSHLAWSESSAVCYVNSVIGARSNREGGPGA
                     LSAAICGRTPLYGYHLESNRKASLVVEVETNLRGADYGALGYLVGKVVGGGVPYFQ"
BASE COUNT          364 a          290 c          351 g          303 t
ORIGIN      
        1 gtccggggaa accggctgca gcgataccct tgtgcgggaa gaactggtca gggtgaatcc
       61 aaagatcctg ggaacagttt attccctgat gcatgaaaag cctccattac gtagcatcga
      121 agactattct cgcatccttg atgcttgtgg aaagagtaaa aaacacaccg ttggtatgag
      181 tatctgtctg agagatcggg atgaggcact catgaagggt gtggagttac tgcaccgcta
      241 ccaggaccga ctgctttctg gcctggaatg gatacggagg gaaggtagcc aggagatgga
      301 atatgtgcag tacatataca ccgaggataa gaaacgcaaa aacattttgg gaaccctggc
      361 cagtgtgggc ttggagttag gtttcctgaa tcccgaaaag ccggtgctga ctctggcccg
      421 tttacatcaa gatgtgaagg tatcagcccg taccactcct caaatgactg ctaaaggtgt
      481 gaatcttgga ttggtcatgg agcaagcctc ccataatttc aatggatctg gtggtgggca
      541 taacattgct gctggtgcca tgattcccta ccaagatctg gaaaacttca aaaacttggt
      601 gaacgatatg gtgggaagcc agatatcttc ataaaacagt ctattttttg gtgatcaaat
      661 tgtacctaaa cccggaagag gagaaaatgt accagggaga ctacggtcct gctgtagaga
      721 agagtatgga aatcctggtg gcgctgggtg atatctacca agccgaaaaa atggtggaaa
      781 ttagctcagc ccaaatatca ggagtgtcct acaagaccat tggcgacgct ggattggaat
      841 ttctagaaga aatggcccag actgcccggg taagagtacc atcaaccctc aacccagctg
      901 gtgttgactt agaccactgg gaggagctgg gtttctccag agaattcact gagagacagc
      961 tgcgaatagt atccgcctac cagaagatgg gaatcaccac cacttgcacc tgcacaccct
     1021 acctggccag caacctaccc acactgggaa gtcacctagc ctggtcagaa tcatcagctg
     1081 tttgttacgt taattctgtt ataggtgcac gcagcaatcg tgaaggaggt ccaggtgcac
     1141 tttccgctgc gatatgtggc cgaacaccac tgtacggcta tcacctagaa tcaaatcgta
     1201 aagccagcct agtggtggaa gtggaaacaa atctcagagg cgctgattac ggtgctctag
     1261 gttatttagt aggtaaagta gtggggggag gagttcccta tttccagg
//
LOCUS       DAOQVW010000503         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_146642_length_1306_cov_2.375699, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000503 DAOQVW010000000
VERSION     DAOQVW010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146642_length_1306_cov_2.375699"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..288)
                     /locus_tag="PKK85_09025"
     CDS             complement(13..288)
                     /locus_tag="PKK85_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26195.1"
                     /translation="MDMCPKCGGNIVHKPFDTCECCQVVGSYHQYEVFEPKLGWLSDN
                     LIKAEMRRCNLELKKFKKTLTNRSIFILNLWYWQGMDGKEIDELCIV"
     gene            complement(339..653)
                     /locus_tag="PKK85_09030"
     CDS             complement(339..653)
                     /locus_tag="PKK85_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26196.1"
                     /translation="MKGMEQLLQTNQRQKLIFGPNRCTVYNYTNIYRQVSDGEERVYA
                     FIFSGDKDRKHDEGYYKLIQVRKLKRVLVTKVEEVPVSKIKGYSSSDWTMKQYRERYF
                     DK"
     gene            complement(706..891)
                     /locus_tag="PKK85_09035"
     CDS             complement(706..891)
                     /locus_tag="PKK85_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26197.1"
                     /translation="MHRMPKEDETMRRAIWIYSCEECMTRQQVGFCPELKRKPLPDST
                     TGFDPLCPLDIVRGHFK"
     gene            complement(858..1013)
                     /locus_tag="PKK85_09040"
     CDS             complement(858..1013)
                     /locus_tag="PKK85_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26198.1"
                     /translation="MTTIRHRPVLLNITGTGHCTQCNSPALILHPVVIDRVEHVLCTE
                     CQRRMKL"
     gene            complement(1010..>1306)
                     /locus_tag="PKK85_09045"
     CDS             complement(1010..>1306)
                     /locus_tag="PKK85_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26199.1"
                     /translation="TKDNIYQVCQKLVKAGKLEITKEQRGAGKAQNIYTIPSVQGYMR
                     DMEDEETTPTPIPKVARSYQFNQQWDELKNRLIKDGDCGTALKIMVTMEEEARP"
BASE COUNT          316 a          309 c          224 g          457 t
ORIGIN      
        1 gattaacgaa gtttaaacta tacataattc atctatctct tttccatcca ttccctgcca
       61 gtaccataaa ttcaatatga aaatggatct gttcgttaag gttttcttaa actttttcag
      121 ttccaaatta caccgcctca tctctgcctt aattaaatta tcagataacc aaccaagctt
      181 aggttcaaat acttcatatt gatgataact ccccactact tgacaacatt cgcaggtatc
      241 aaaaggttta tgaactatat tccccccaca ctttggacac atatccattt gtttcccctc
      301 tctttttttg ttcattgacc cgcatataac gcagttaatt atttatcaaa atatctttcc
      361 ctatattgtt tcattgtcca atcactagat gaatacccct taattttaga tactggcact
      421 tcttcaacct tagttactaa tactcttttt aatttcctaa cttgaattaa tttataatat
      481 ccttcatcat gttttctgtc cttgtctcct gaaaaaataa aagcatatac ccgttcctcc
      541 ccatctgaaa cttgacggta tatattagta taattataaa cagtacaacg attagggcca
      601 aatattaatt tctggcgttg gttcgtttgt agcaattgtt ccattccctt cattgttcac
      661 cccttttttt cttcgttata gtctggatta acgaagtata attctttatt taaaatgccc
      721 cctgactata tcaagtgggc aaagtggatc aaaaccggtg gtgctgtctg gcaggggttt
      781 gcgtttgagt tccgggcaga accccacctg ttgccgtgtc atgcattctt cgcagctata
      841 aatccagatg gcccttctca tagtttcatc ctcctttggc attcggtgca taatacgtgc
      901 tccacccggt ctattaccac agggtggaga ataagggcgg ggctgttgca ttgtgtgcag
      961 tggcccgttc cagtgatgtt caataggacg ggccggtggc gtatcgtggt catggtcttg
     1021 cctcctcttc catcgttacc atgattttaa gggcggttcc acaatcccca tctttaatta
     1081 atcggttctt caactcatcc cactgctgat taaactggta actcctggcc acttttggta
     1141 taggtgtggg ggttgtttct tcgtcttcca tgtcccgcat atacccctgc acactaggga
     1201 tagtgtagat gttctgtgct ttaccggctc cacgttgttc cttggtgatt tccagcttac
     1261 ctgccttaac cagtttctgg cagacttggt agatgttatc cttagt
//
LOCUS       DAOQVW010000504         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_147410_length_1302_cov_1.981556, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000504 DAOQVW010000000
VERSION     DAOQVW010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147410_length_1302_cov_1.981556"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1244)
                     /locus_tag="PKK85_09050"
     CDS             complement(174..1244)
                     /locus_tag="PKK85_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNS26200.1"
                     /translation="MATQLIKGNTAVIIGAMYAGCDCYFGYPITPATEILHEASKYFP
                     MVGRKFVQAESEEAAINMVYGASAAGHRVLTASSGPGISLKQEGISFLAGAELPCVIV
                     DVMRAGPGLGNIGPEQADYNQLVKGGGHGNYRNIVLAPNSVQEMCDLTIKAFDLAEKY
                     RNPVVIMADGVLGQMVEPLKFPEEAIKPQIDAGWAVCGTKETMDNLVTSIFLDFDQLE
                     DFNFRLQEKYQEIEENEVDFEEYQMEDAEIILVAYGISSRISRSVVEQVRKEGIKAGL
                     FRPKTLFPFPEERLKEYAQKGETKFISVEMSNGQMREDIILSIGCSRPVELVNRMGGN
                     IMDVESIIQKIREVDSKRAGGE"
BASE COUNT          249 a          344 c          271 g          438 t
ORIGIN      
        1 cgctgtaggt gcatcgcccc cttttctagg aaattcgctg agcatggatt ctggtttgcc
       61 tattattttc tcatcctgtg aattttcttc agatttgaca tcgtccacct ggtcattcgt
      121 ttttaactca cccctgttta actcatcatc ccggtttacc ttttcatcca tattcattca
      181 cctccggctc tttttgaatc tacttctctg attttttgta tgatactttc tacatccatt
      241 atatttccgc ccatacggtt taccagttcc accgggcggg aacatccaat tgataagatt
      301 atatcttccc tcatctgacc attactcatc tctactgaga tgaatttagt ttcacctttc
      361 tgggcatatt ctttaagcct ctcctctggg aaggggaata gggtttttgg tctgaagagt
      421 cctgccttta ttccttcctt tcttacctgc tcaacgactg atctggatat cctgctactt
      481 ataccgtagg ctaccagtat tatctccgca tcttccatct ggtattcctc aaagtccact
      541 tcattttcct ctatttcctg gtacttttcc tggagtctga agttgaagtc ctccagctgg
      601 tcgaagtcta agaatatgga agtgaccagg ttatccatgg tttccttggt cccgcagaca
      661 gcccagcccg catcgatctg gggcttgatt gcttcttccg ggaatttaag tggctccacc
      721 atctggccga gcactccgtc ggccatgatc accacggggt tgcggtattt ctctgccaga
      781 tcgaaggctt tgatggtgag gtcgcacatc tcctggacgg agttgggggc taagactatg
      841 tttctgtaat ttccatgtcc tcctcctttc accagttgat tgtagtctgc ctgttctggc
      901 cctatatttc ccagtcccgg gccggcccgc attacatcca caattacaca gggtagttcc
      961 gcccctgcaa ggaaggatat tccctcctgt ttcaggctta ttcctggtcc ggaggaggca
     1021 gttaacactc gatgtccagc ggctgaggct ccgtagacca tgtttatggc tgcttcttct
     1081 gattcagcct ggacaaattt cctccccacc atggggaagt attttgaagc ttcatggagg
     1141 atttcagtgg ctggtgtgat ggggtatccg aagtaacagt cacatccagc gtacatggcc
     1201 ccaattatca ctgcggtgtt tcctttaatt agctgggtgg ccatttactc atcctcctca
     1261 gttgttgttt tgcctttcct ggcttttttt ggtatatgaa ct
//
LOCUS       DAOQVW010000505         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_147561_length_1301_cov_2.302568, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000505 DAOQVW010000000
VERSION     DAOQVW010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147561_length_1301_cov_2.302568"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /gene="dph2"
                     /locus_tag="PKK85_09055"
     CDS             <1..776
                     /gene="dph2"
                     /locus_tag="PKK85_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HNS26201.1"
                     /translation="LVHFGHTPLPLEYDVPVLFLEAEFQLDVTPAVIKALDLLKDYKK
                     IGLVTTAQHLHLIDEITGILQENGKEVIMKEGRGTQKAQVLGCNFSSIENLSIDAFLF
                     LGSGNFHPLGIKLSTDKPVIIADPYHNEARNIHEFTDRMMRIRFAKITKAREGERFGI
                     IVSSKKGQYRIELAQSLKDTIEDEGKQAFIIFMNQVTPELLVPYRDLDAFVVTACPRI
                     AIDDANIYDKPLLTPEELLIVLDKKEWGDYVMDEIRYED"
     gene            931..>1301
                     /locus_tag="PKK85_09060"
     CDS             931..>1301
                     /locus_tag="PKK85_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26202.1"
                     /translation="MTIVKSFAPISCLNHKQYKLSDFSESLSKYEFEQGKERNKTNKY
                     DNSLVMYFKKDTLYIEDNFGVCPSCYSKSVSFDGYNDRLLYFYDEGAVNAKIKRYTCD
                     NCGNNFITDLNEVVEPGCNYT"
BASE COUNT          424 a          225 c          272 g          380 t
ORIGIN      
        1 tactggtcca tttcggccac actcccctac ccctggaata cgacgtgcct gtgctttttt
       61 tagaagcaga attccagctg gacgtcacac cagccgtcat taaagcttta gatctcttaa
      121 aagattataa gaaaatagga ctggtgacca ccgcccagca ccttcacctt atagatgaaa
      181 taacgggtat actacaggaa aatggtaagg aagtgattat gaaggagggc aggggaaccc
      241 agaaggccca ggtactggga tgtaactttt ccagtataga aaacctatcc atcgatgcat
      301 tcctgtttct aggtagtggg aacttccacc ccctgggaat caaactctcc acagataaac
      361 cggtcatcat cgccgatcca tatcataatg aggcgaggaa tatccatgaa ttcaccgacc
      421 ggatgatgag gataaggttc gctaagatca caaaagcccg ggagggggaa agatttggaa
      481 tcatagtctc ttccaagaag ggacagtacc gtatagaact ggcccaaagt ttaaaggaca
      541 ccatcgaaga tgaaggaaaa caggcgttca tcatcttcat gaaccaggtt actccggaac
      601 tcctggtacc ctacagagat ttagatgcat ttgtggtaac tgcctgtccc aggatagcaa
      661 tcgatgatgc aaatatatat gataaacccc ttttgacgcc ggaagagcta ttaatagttc
      721 tggataaaaa ggaatggggc gattatgtga tggatgagat aagatatgag gattgaaact
      781 catttcaaag aattttcggc tctgtaaaat attggttgtt ttttgatttt tatattttct
      841 tgttttgatt tttgtatata gttgtatatg tgtttatttt gtaaaataga agagtaaaat
      901 taatataaag gggggagcaa atattttagc atgacaatcg taaaatcatt tgctcccatt
      961 agttgtttga atcataaaca atataagctt tccgattttt ctgaaagttt atcgaaatac
     1021 gaatttgaac aaggtaaaga aagaaacaaa actaataaat acgataattc gcttgtaatg
     1081 tattttaaaa aggatactct atatattgag gataattttg gtgtttgtcc cagctgttac
     1141 tctaaatccg tttcttttga tggatataac gacagattac tctactttta tgatgaagga
     1201 gctgtaaacg ctaaaattaa aagatataca tgtgataact gtggcaataa ctttatcaca
     1261 gacttgaatg aggttgttga acctggttgt aattatacaa g
//
LOCUS       DAOQVW010000506         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_147703_length_1300_cov_2.844177, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000506 DAOQVW010000000
VERSION     DAOQVW010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147703_length_1300_cov_2.844177"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKK85_09065"
     CDS             <1..794
                     /locus_tag="PKK85_09065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS26203.1"
                     /translation="TYNSGNKPIQVTELTEKQIMTNTRDLIHKPSDDGSAVQNKLTII
                     RNQENHGFAVGNNIAIGYVMENFNPEYILLLNNDTVVDPGFLTNLVEVAEKDEKIGLL
                     GPLIYYYDVDGQSDVVANLGGKLDLSHYPGYYDLVEFNQLEDFPGPLIECDWISGAAL
                     LMKTREIPIHTLNPELFFGCEDIDLAIKLNKKGFKSVVVLNSYIWHKEAVSRKKRSPD
                     SVNRALMEIKSNLTFLKTHNKHYYRYLPFYTIQIIKLYLKVLLKG"
     gene            complement(809..>1300)
                     /locus_tag="PKK85_09070"
     CDS             complement(809..>1300)
                     /locus_tag="PKK85_09070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS26204.1"
                     /translation="KKRMDNVVLFILGEGECRDELESISHNLELGKDVHFTGNIHNDL
                     LGAYYLLCNVFVLPSVTTYYADACPLVVNEAMYFAKPVVTSDAVGNTFMIKEGINGFV
                     VEERNTEALCGALHRILSDDALEEKMGKASKEIIKEGFTYQNMMEGFNQALEYVYRNE
                     KEK"
BASE COUNT          391 a          309 c          253 g          347 t
ORIGIN      
        1 tcacctacaa cagtgggaac aagcccatcc aggtcacgga gttaactgaa aagcaaataa
       61 tgaccaacac cagagatctg atacacaaac catctgatga tggatccgcg gtccagaaca
      121 agctcaccat catcagaaac caggaaaacc atggattcgc ggtgggaaac aacattgcca
      181 ttggctacgt tatggaaaat tttaacccag aatacattct ccttttaaac aacgatacag
      241 ttgttgatcc aggatttctg actaacctgg tggaggtggc agagaaggac gagaaaatcg
      301 gacttctggg gccattaata tattactatg atgtggatgg ccagagtgac gtggtggcta
      361 acctgggggg aaagcttgat ctctcccact acccgggtta ctacgatctg gtggaattca
      421 accaactgga agactttccg ggccccctaa ttgaatgtga ctggatctca ggggcggccc
      481 tcctcatgaa aaccagggaa atacctatcc acaccctgaa cccggagctt ttttttggat
      541 gcgaagatat tgacctggcc attaaactga ataaaaaggg atttaaatct gtagtggttc
      601 taaattcata catatggcat aaagaagcag tttcccgaaa gaaaagaagc cccgattcag
      661 tgaacagggc tttaatggaa attaaatcca acttaacctt tctaaaaacc cataacaagc
      721 attactaccg ttacttaccc ttttatacca ttcaaataat taaattatac ctgaaagttt
      781 tattgaaggg ctaaaacggc cataataatt atttttcctt ctcattccta taaacatatt
      841 ccagggcctg gttgaaacct tccatcatgt tttgataggt aaatccctcc tttattatct
      901 ctttagaagc ttttcccatc ttctcctcca gggcatcatc agacagtatc ctgtgcaatg
      961 ccccacagag tgcttcagtg tttctttcct ccaccacaaa cccatttatc ccctctttga
     1021 tcatgaaggt gttccccact gcatcactgg taaccactgg ctttgcaaag tacatggcct
     1081 cgttaaccac caggggacag gcatcagcgt agtaggtggt tacagagggc agaacaaaga
     1141 cgttgcataa caagtagtag gcacctagaa gatcgttgtg gatgttcccg gtaaaatgaa
     1201 catcttttcc taattccagg ttatgggaaa tactttccaa ctcatcccta cattcaccct
     1261 cgcctaagat gaacaagact acgttatcca tccttttttt
//
LOCUS       DAOQVW010000507         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_148237_length_1297_cov_3.676329, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000507 DAOQVW010000000
VERSION     DAOQVW010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148237_length_1297_cov_3.676329"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..334)
                     /locus_tag="PKK85_09075"
     CDS             complement(137..334)
                     /locus_tag="PKK85_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26205.1"
                     /translation="MLSISVAMLDFSWIPEIDSAQTLISHSREVLEGTGFISSFLRNL
                     LTFPIRWFYGYHHCHDVKGGS"
     gene            complement(479..>1297)
                     /gene="aroE"
                     /locus_tag="PKK85_09080"
     CDS             complement(479..>1297)
                     /gene="aroE"
                     /locus_tag="PKK85_09080"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNS26206.1"
                     /translation="GIIGDPVEHSLSPPMHNAAFTHLMMDYVYVPFHVRDENLESAIE
                     GARSLELKGLNVTIPHKTAVMPYLDEVDQAAQLIGAVNTIKFSKNKAKGYNTDGVGAV
                     RALEEIIPVKDKRVVIMGAGRAARALAFQLILNGIGELAIANRTREKAVQLAHDIQGK
                     MEFSPEVTGLGEVELSTTDILINTTSVGMHPHQDQPPLLRAEKMHPGLVVNDLIYNPL
                     KTNLLKEAEKIGARTLNGTKMLIYQGMESFRIWTGVTPPVEVFETSLIRILGKI"
BASE COUNT          296 a          326 c          314 g          361 t
ORIGIN      
        1 cggtccaggt gggcttgtta ggtatgatca tggcggtgcc accggagcgg tagaatttct
       61 gggccacagg aagcggcccc tcaccattga tgggatccac gtggatgtgg ttatctgtgg
      121 atggaatcat caccatttaa gatcctccct ttacatcatg acagtggtgg tagccgtaga
      181 accatctaat cgggaaggtg aggaggttcc ttaaaaagga agatataaag cctgtgccct
      241 ccaggacctc tctggaatga gatatcaggg tctgggccga gtcaatttcc gggatccagg
      301 aaaaatccag catggccact gatatactaa gcacccccta tccgggtgga taagttttcc
      361 tgctctccta cctcatattt ccattttact tccatcaaat cagccccatt aatgaattct
      421 aagctatggt tggtttttgt aaaattccac tattaaaagg gagtctttca gaggggcact
      481 aaatcttgcc caggattcta atcaggctcg tttcaaacac ttccactggt ggtgtgactc
      541 cggtccagat gcggaaggac tccatcccct ggtatatcaa catcttcgtg ccgtttaatg
      601 ttcttgcacc tattttttct gcttccttca ggagattggt cttcagtggg ttgtagataa
      661 gatcgttaac caccaaccca gggtgcattt tttcagccct aagcagtggg ggctggtcct
      721 ggtgggggtg catacccacc gaggtggtgt ttataaggat gtcagtggtg gaaagttcca
      781 cttcccctag accggtgact tccggggaaa attccatttt accctgaata tcatgggcca
      841 attgcaccgc cttttcccgg gtgcggttgg cgatggccaa ttcaccaatt ccatttaaaa
      901 ttagctggaa agccagagcc cgagccgctc tcccagcacc cataataacc actctcttat
      961 cttttacagg tattatctct tccaaggccc tcaccgcacc tactccatcg gtgttgtaac
     1021 ctttagcctt gtttttactg aatttgatgg tgttcacagc cccgatgagt tgggcggcct
     1081 ggtccacttc atccaggtag ggcatcaccg cggttttatg gggtatggtg acgttcaacc
     1141 ccttaagttc aagagaccgg gccccttcta tggcactctc caggttttca tcccttacat
     1201 ggaagggaac atacacataa tccatcatca gatgtgtgaa tgccgcgttg tgcataggag
     1261 gggataggct gtgttccaca gggtcgccta taatgcc
//
LOCUS       DAOQVW010000508         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_148923_length_1294_cov_1.996772, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000508 DAOQVW010000000
VERSION     DAOQVW010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148923_length_1294_cov_1.996772"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PKK85_09085"
     CDS             <1..1224
                     /locus_tag="PKK85_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS26207.1"
                     /translation="KGLSSVIKGIAIFGVKKPFTPGAPFQVVWNLSRACNMKCVHCYE
                     NAGRKDEDELNTQQINDGLETMAKAGVTSIAFSGGEPSIHPHILDFIDKTNELGMFAA
                     MATNGYTLSKEGVCEKFVENGLQFVQISVDGLQAETHDSFRGINGAWDKAIHAVENCV
                     NYNDLFVEVATTVTEHNIQEIPEMIDFMRDKGVDWFMIYNFIPTGNGENIASMDISPK
                     KRLKLLETAYDENAEGEMQVLSTAPQYAMVAESLVSDGNQVIPTHFYNPEYDGFLIKQ
                     LADFIGGCGAGRFYMSVEPNGDLYPCVFFPHDSALKLGNLMEDDFEEVWKDNQLLSKL
                     RNKEILKGHCGDCESHNICGGCRARAYNYFGDILAPDPGCINNNKEWNKIRSHLPKET
                     KQLNDGSLFIDLNSK"
BASE COUNT          418 a          272 c          306 g          298 t
ORIGIN      
        1 aaaggattaa gcagtgtaat taagggcatc gctatttttg gagttaagaa acccttcact
       61 cccggggcac cctttcaggt agtctggaac ttgagccggg cctgtaacat gaaatgcgtg
      121 cactgctatg aaaatgcggg aaggaaggat gaagacgaat taaacaccca gcagattaat
      181 gatggtctgg aaacaatggc taaagctggt gtgacctcca tcgccttctc cgggggagaa
      241 cccagcatac acccccatat actggacttc attgataaaa ccaacgagct gggaatgttt
      301 gcggccatgg ccaccaacgg ctacaccctt tctaaggagg gagtgtgtga aaaattcgtg
      361 gaaaatggcc ttcaatttgt acagataagt gtggacggcc tccaggcgga aacccacgac
      421 tcatttagag gtataaatgg tgcctgggat aaggcaatcc atgccgtgga aaactgtgta
      481 aactacaacg atctatttgt ggaagtagcc accacagtca cagaacataa catccaggag
      541 ataccagaaa tgatagactt catgcgggat aaaggcgtcg actggttcat gatctacaac
      601 ttcataccca ccggaaatgg tgaaaatatt gcaagcatgg atatttcccc caaaaaacgg
      661 ttgaaactac tggaaacagc ctatgatgaa aatgcagaag gtgaaatgca ggtcctctcc
      721 accgcaccac aatacgccat ggtagctgaa tcactggtct cggatggtaa ccaggtaatt
      781 cccactcact tctacaatcc agaatatgac ggattcctta taaagcagtt ggcagatttt
      841 ataggcggct gcggtgctgg aagattctat atgagcgtgg aacccaacgg agacttatac
      901 ccctgtgtct tcttcccaca tgactcggcg ttgaaactgg gaaacctgat ggaagatgat
      961 tttgaagagg tctggaaaga taatcaatta ctgagtaaac tcagaaacaa agaaatcctg
     1021 aagggccact gtggtgactg cgagtcccac aatatatgcg gaggatgcag agcccgggcc
     1081 tataattact tcggggatat attagcaccc gatcccggtt gtatcaacaa caataaggaa
     1141 tggaataaaa ttaggagtca cttgcccaaa gaaactaaac aacttaacga tggcagctta
     1201 tttatcgatt taaatagtaa ataggaaaaa agttagatga ttatgatgga tacgttatta
     1261 atcaacccct acgatgaaaa tgcagtgaaa aatg
//
LOCUS       DAOQVW010000509         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_149016_length_1293_cov_3.105008, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000509 DAOQVW010000000
VERSION     DAOQVW010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149016_length_1293_cov_3.105008"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PKK85_09090"
     CDS             <1..613
                     /locus_tag="PKK85_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNS26208.1"
                     /translation="KLRDEVHQEYKKMEWASGRREIVKIQDEIKRLEKTIETRVLDIR
                     KENELVNKVTDLRKNLQTLQEDEETREEALELKEKSESYHAKVVELSDSAQETHEKML
                     EYFRKIDKIRSQADEAHQKFIQTRDTANQEHEKVKSTLGDIRRLNKGLDRVKAKERNR
                     ETEIVRQQNKEEKERAEDIYRKFREGKKLSTEELLLLQKHNIV"
     gene            704..>1293
                     /locus_tag="PKK85_09095"
     CDS             704..>1293
                     /locus_tag="PKK85_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26209.1"
                     /translation="MAEVNSTPKSDKNQIKIAALLVASIASFFTPFMSSSINIALPTI
                     GANFAADAVLLNWVANGFLLSAAIFAVPFGRIADIYGMKKIFAYGMIIFTIASLLCAL
                     SPNTLSLIGARVLQGIGTAMIFVTGLAIITSVYPPHQRGKAIGINVASVYVGLSIGPV
                     LGGLMTQYLGWRSLFLLMIPFGLLVIFLVYYKLHDE"
BASE COUNT          403 a          254 c          288 g          348 t
ORIGIN      
        1 aaaactgcgg gatgaagttc atcaggaata caagaagatg gaatgggctt caggcaggag
       61 agaaatagtt aaaatccagg acgagattaa acgcctagaa aaaaccatag aaacccgcgt
      121 actggatatt agaaaggaaa atgagttggt gaataaggtt accgatctca ggaagaatct
      181 gcagaccctc caggaagatg aagaaacccg tgaggaagca ctagaactca aggaaaaatc
      241 ggaaagttat cacgccaaag tggtggaact ttctgatagt gctcaggaaa cacatgagaa
      301 gatgttggag tacttccgga agattgataa aatacgatcc caggccgatg aagctcatca
      361 aaaattcata caaaccagag acaccgcaaa ccaggaacat gaaaaggtca aatctactct
      421 gggagatatt agaaggctga acaagggact tgatcgtgta aaagccaagg aaagaaaccg
      481 ggaaaccgaa attgtaaggc agcagaacaa ggaagaaaag gaacgagccg aagatattta
      541 tcgcaagttc cgggaaggta aaaaactttc caccgaagag ttgctgctcc tgcagaagca
      601 caacattgta taaagggcta acttgccccc tccatacttt taggattttt ttatccattt
      661 ttttatccat atttatcatt attatattat accagtggtt ttaatggccg aagttaattc
      721 tacccctaaa tctgataaga atcagattaa aatagcagca cttctggtgg catctatagc
      781 atcatttttc actcccttca tgagttcttc cattaacatt gccttaccca ccataggggc
      841 caacttcgct gctgatgctg ttcttttaaa ctgggtagcg aatggttttt tattatcagc
      901 agccatattt gcagttcctt ttggaagaat agctgatata tatggtatga aaaagatttt
      961 tgcctatgga atgattatat tcaccatcgc ttcattgtta tgtgctttat caccgaacac
     1021 cctttctctc attggtgccc gggtgttgca aggcattgga accgccatga tcttcgtcac
     1081 agggctggct atcattacct ctgtgtaccc accacaccaa cggggtaaag ccattgggat
     1141 caacgttgct tcagtttacg ttggtctgtc catagggccc gtattaggtg ggctgatgac
     1201 ccagtatctg gggtggagaa gcctgttctt actgatgatt ccatttggac ttttagttat
     1261 ttttctggtg tactataagt tgcatgatga atg
//
LOCUS       DAOQVW010000510         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_149238_length_1292_cov_2.555376, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000510 DAOQVW010000000
VERSION     DAOQVW010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149238_length_1292_cov_2.555376"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKK85_09100"
     CDS             <1..436
                     /locus_tag="PKK85_09100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26210.1"
                     /translation="VPTVILVPIIFSGYDIPRKLFIWLPSVIYAFLAGAILYANQFSL
                     WFLMIIAGIINILRFNTLLTLPVEIMPREHAGTASGVVVSVGYMGAVVGPIIAGEILD
                     VSGSLQSIFIILIVLSLITTVFAFLIPSDNTKKRSIQIDQKN"
     gene            complement(460..1239)
                     /locus_tag="PKK85_09105"
     CDS             complement(460..1239)
                     /locus_tag="PKK85_09105"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNS26211.1"
                     /translation="MKFVRFKKDGVRNTGILEDDSITELSCSMKKAIDAQSIGKFVLE
                     KCTYDLKEVEILPPVSPTKVVCVGLNYVDHARELNMEIPEEPVIFIKPSTGVIGPLDS
                     IIYPSSSKQVDYEAELALTISTKAHNIKKEDADQYIGGYTVLNDVTARDFQRKDGQWT
                     RAKSFNTFCPIGPCIETRLDPADQQISLKLNGEVKQNSNTRNMIFSPQELVEFVSNIM
                     TLHPGDIIATGTPPGVGPMNVGDTVEVEIENIGSLKNFVTK"
BASE COUNT          341 a          252 c          265 g          434 t
ORIGIN      
        1 tgtccctacg gtgatattgg tgccgataat attttctgga tatgatattc cacgaaaatt
       61 gttcatatgg ttacccagcg tgatatacgc attcctagca ggtgcaatat tgtatgccaa
      121 ccagttttcc ctctggtttc tgatgatcat tgcaggaatc ataaatatcc tgcgtttcaa
      181 tactttgcta actttgccgg ttgaaataat gcccagagag catgccggta cagccagtgg
      241 tgtggtggtg tcagttggtt atatgggggc tgtggttggg cccatcatag ccggggaaat
      301 tctggatgtc agcggaagtt tacagagtat tttcattatt ttgatagttt tatctttgat
      361 aaccaccgta tttgcctttc taattccatc agataatact aaaaagcgct ccatccaaat
      421 agatcagaaa aattaatctt tattttgtaa taaaaacttt cattttgtaa caaaattctt
      481 aagggatccg atattttcga tttctacttc cacggtgtca ccaacattca tgggaccaac
      541 accgggtgga gttccggtgg caattatatc gccaggatgc aaagtcatta tgttagatac
      601 aaattccacc aactcctggg gtgagaagat catatttctg gtgttagaat tctgtttcac
      661 ttcgccattg agtttgagag atatttgctg gtcagcaggg tctagtctgg tttcgatgca
      721 tggtccgatg ggacagaagg tgttgaaact cttggctcgt gtccactgtc catcctttct
      781 ctgaaagtct ctggcagtga catcattcaa gacagtgtag ccgcctatgt actggtcagc
      841 atcttctttt ttaatattat gggcttttgt ggaaattgtt agggccaatt cagcttcata
      901 atccacctgt tttgatgatg atggatatat tatggaatct aaaggaccaa taactcctgt
      961 agatggttta ataaatataa ctggttcctc agggatttcc atattcagtt cccgagcatg
     1021 atccacgtag ttaaggccca cacagaccac ttttgtcgga gaaactgggg gaagtatttc
     1081 aacttctttc aggtcgtagg tacatttttc taaaacaaac ttacctatgc tctgggcatc
     1141 tatggctttt ttcatggagc aggataattc agttattgaa tcatcctcta aaatccctgt
     1201 atttctaact ccgtcctttt taaagcgtac gaatttcatg taaacaccga ttaaaatgtt
     1261 gggcttttaa agtcctaaat tattctttca at
//
LOCUS       DAOQVW010000511         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_149838_length_1289_cov_2.324959, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000511 DAOQVW010000000
VERSION     DAOQVW010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149838_length_1289_cov_2.324959"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1127)
                     /locus_tag="PKK85_09110"
     CDS             complement(9..1127)
                     /locus_tag="PKK85_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HNS26212.1"
                     /translation="MDKKLIAGIIIIAILVGGILVYTFGGDIPVSREGNVSITDAAGR
                     TVQVPAQVNKVVGTGCSGREIVYLNASDKMVGIEQMESNSTGAWGNQLPYMIAHPELL
                     KLPVVANARTDTVYYEKLAEVDPDVVFVGTAEQADNVQTKTGIPAVVVYVGAVETPEQ
                     MDKYKESLKMMGKVLGKEDRADELINYINSYEEDLAKRTAGVSSNKTVYIAGQAYYGT
                     HGITSTNAFYPPFIMVNADNVASAMNTTTNATLHAISVDKEQLVTWNPDMIFVEGSSA
                     ESIINDTNQNTVYQSINAVKNGDVYDVLPYCLFSYNKDSMIADAYYIGKILYPEQFAD
                     VDPEEKANEIFMKFNGGSGELVYSTLKTQYGGFKKMDI"
BASE COUNT          336 a          300 c          236 g          417 t
ORIGIN      
        1 gggtgcaatc agatgtccat cttcttgaat ccgccgtact gagttttcaa cgtgctgtaa
       61 accagttcac ccgatccacc attgaatttc atgaatatct cgttggcttt ttcttctgga
      121 tctacatctg caaattgttc aggatagaga attttaccga tataataagc atcagcaatc
      181 atcgaatctt tattgtaaga gaacaagcag tatggaagga catcgtagac gtcgccattt
      241 tttacagcgt ttatgctttg ataaactgta ttctgattgg tatcgttgat tatcgattct
      301 gcactgctcc cctctacgaa gatcatgtca ggattccagg ttactagttg ctctttatcc
      361 actgaaattg catgtaaagt tgcattggtg gtggtgttca tggcactggc cacgttatct
      421 gcattcacca ttataaatgg gggataaaat gcattggtag atgtgatgcc gtgtgttcca
      481 taataagcct gtccagctat gtatactgtt ttgttgctgg agacgccggc cgttcgttta
      541 gcaagatcct cttcgtagga attaatatag ttaatcagtt catcggccct gtcttcctta
      601 cctaacacct tacccatcat cttcaaagat tccttatact tatccatctg ttcaggtgtt
      661 tcaaccgcgc caacgtatac cacaacagca ggaatacccg ttttggtctg tacgttatct
      721 gcctgttctg cagtgccaac aaacaccaca tcggggtcaa cttcggctaa tttttcatag
      781 tatactgtat cggtccttgc attggcaaca actggtaatt tcagaagttc gggatgagcg
      841 atcatatagg gcaactgatt tccccacgcc cctgttgaat tggattccat ctgttctatt
      901 ccaaccattt tgtcactggc gtttaaatac acaatttctc taccagaaca tccggttccc
      961 accactttat taacctgggc tggaacctgg actgttctac ctgctgcgtc tgttatggaa
     1021 acgttgcctt ctcttgaaac tggaatatct ccaccaaagg tgtagactaa aattccgcca
     1081 accagtattg cgatgataat aataccagct attaactttt tatccataaa atcaccctta
     1141 atttatcctt aaagtacctt caaaaaatgg gaaaaaaatt aaatctctat cttttcaaat
     1201 ccaccaagcg gtccctttag atcactgtag actggtttac ccacgaacat cgtgaatatt
     1261 tcatctgctt tcttttcggg gtctacatc
//
LOCUS       DAOQVW010000512         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_149922_length_1288_cov_4.047851, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000512 DAOQVW010000000
VERSION     DAOQVW010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149922_length_1288_cov_4.047851"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..563
                     /locus_tag="PKK85_09115"
     CDS             264..563
                     /locus_tag="PKK85_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS26213.1"
                     /translation="MVKTTPADTILEKMDPEVQNAFKKLAQAIHKEGALSTREKTLIA
                     LASSVAIRCQDCVKRHAKLATQAGASQEELLEAAALASLVRMGSGLNTASTIVKD"
     gene            727..>1288
                     /locus_tag="PKK85_09120"
     CDS             727..>1288
                     /locus_tag="PKK85_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HNS26214.1"
                     /translation="MKYKALVLILVGLGVLAVMVLFIGPAKIEDAIKLADPWYLALAV
                     ILQFVTYGLWTLRWSINIQSVGLSIENRDLLPMLLVGLAVNNITPSARGGGEPVRGYI
                     LSKYTASSFEKAFATVIADRALDTIPFIVLSVITIIAAASHLNLPGYGLYSITIAVVV
                     LVSGFLVLLYMAFNLEFGRKVSLWIAR"
BASE COUNT          340 a          309 c          303 g          336 t
ORIGIN      
        1 gtgataaaaa cctggacata gaactgctgc gaggcctgaa atgaggtgtt ggccttaatt
       61 ttaaggggat aggaatctgt tcatggtggt tagggcatta tctaccggtc aaagtgggag
      121 caacaccagg gcgccaaggt tgacaccaga tctgactgcc tgataaaatc ttgtccccta
      181 gtacaccata tcagttaatg aaactatagg ttcttcattt taaaaaccat aaattaattt
      241 accagaacaa taaagttgct actatggtta aaactacccc tgcagatact attctagaaa
      301 aaatggatcc ggaagttcag aatgccttca aaaaactggc ccaagccatc cacaaggagg
      361 gagccctatc cacccgggag aaaacactta tagcattggc ctcttcagtg gccattcgtt
      421 gtcaggactg tgtgaagcgg catgctaagc tggccaccca ggccggtgcc agtcaggaag
      481 agcttttaga agccgcagcc ctagctagtc tggtgaggat gggctctggt ttaaacacag
      541 cctccaccat tgtgaaagat taataaacta tatcaaccat ccccctatac taaacataaa
      601 ccatggttca gatatttttt gattaacaac ctttaaccat ccctttctat taaataggcc
      661 ttaattactg gaaatggtaa cagaatttgc cattggtgtt gatgtgataa tcaggaggtt
      721 tgtatagtga agtacaaagc cctagtcctg atcctggtgg gactcggagt cctggccgtc
      781 atggtcctct tcattggacc ggctaagata gaggacgcca ttaaactggc tgacccctgg
      841 tacctagccc tggctgtgat cctccagttt gtaacctacg gcctgtggac tttaagatgg
      901 tccattaaca tccagtcagt ggggttgtcc attgaaaatc gggaccttct ccccatgctc
      961 ctggtgggcc tggccgttaa caacatcacc cccagtgccc gggggggagg agaaccagtt
     1021 cggggttaca tactgagtaa gtacactgca tcttcctttg aaaaggcctt tgccacggtc
     1081 atcgctgacc gggccctgga taccatcccc ttcattgtgt tatcggtgat caccatcata
     1141 gccgcggctt cacatctgaa tttaccaggc tatggattgt actccatcac catcgcagtg
     1201 gtggtactgg tgtccgggtt cctggtacta ctgtacatgg ccttcaacct ggaattcgga
     1261 cgtaaagtat ccttgtggat agcaaggg
//
LOCUS       DAOQVW010000513         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_150398_length_1286_cov_2.302193, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000513 DAOQVW010000000
VERSION     DAOQVW010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150398_length_1286_cov_2.302193"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKK85_09125"
     CDS             complement(<1..408)
                     /locus_tag="PKK85_09125"
                     /inference="COORDINATES: protein motif:HMM:NF019380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HNS26215.1"
                     /translation="MLDINKLQKGFNIKNWMFTVIFFLILTDLSIILDIPFLREVFSF
                     IFFSVVPGFLVILILNLKGMNPVKKVVLSVGMSISFLMIIGLALNSLYPLLNQPLSFT
                     PVFVTLNLFVVLLSVAAYWRNRNVLEDDFGFKLG"
     gene            complement(495..>1286)
                     /locus_tag="PKK85_09130"
     CDS             complement(495..>1286)
                     /locus_tag="PKK85_09130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS26216.1"
                     /translation="HNDIIGQGLASLVARIYNSVGLNYVLKTAAKIIITQPGYLQSSS
                     HLAKYRDKIEVIPNGVDVEKFQPKQASGNEDKSTIFFLSVLDEFHKYKGLDYLLEALK
                     IVKNNVPDVKLIVGGKGVLLDHHQEMAASLGLKDNVEFAGFIPDEEIADYYSQASVFV
                     LPSISSLQEGFGIVALEALACQTPVVTTDIVGVAHDLKQIKGGIVIPPRDTHKLADAI
                     TQILSDAQMQKEMGQRGRKLVQEKYTWKVVASSMEKVYKEILAKS"
BASE COUNT          374 a          273 c          238 g          401 t
ORIGIN      
        1 acctaattta aagccaaaat catcttctaa aacgttccgg ttacgccagt aggcagccac
       61 agacagtaga acgacgaaga ggtttaaggt aacaaaaacc ggggtgaagg ataatggctg
      121 gtttaacagg ggatataaac tgtttaaagc caagccgata atcatcagga aggaaatact
      181 cattcccacc gatagtacaa cctttttaac agggttcatc cctttgagat tcaatattag
      241 tatgaccaaa aagccaggga ctacagaaaa gaatataaag gagaatacct ctcttaaaaa
      301 gggtatatct aaaattatgg atagatcagt taatattagg aagaatataa ccgtgaacat
      361 ccagttttta atgttgaaac ccttctgtag cttatttata tccaacattc aaaccatccg
      421 cactccttat taaatttaat attatatcct atttccttta aaatattcaa tctaaaaaat
      481 aagattatgt gaatttagga ttttgccagt atttccttat atactttctc catactactg
      541 gccacaactt tccaggtgta tttttcctgc accagttttc tgcccctttg acccatctct
      601 ttttgcatct gggcatcact gagtatctgc gttatagcat cggccagttt atgggtgtcc
      661 ctgggaggaa ttactattcc acctttgatt tgttttagat catgggccac ccctacaata
      721 tcggtggtta ccactggtgt ctggcaggcc agtgcctcca gtgccactat tccaaatccc
      781 tcctggagtg aggagatgga tggtaggacg aatacgcttg cctggctgta ataatctgca
      841 atttcctcgt cagggataaa cccggcaaat tccacgttat cctttaaccc caatgaagcg
      901 gccatttcct gatgatgatc cagtaaaaca cctttacctc ccacgattaa ttttacatca
      961 ggcacgttat ttttaactat tttaagggct tctaaaagat aatccagtcc tttatattta
     1021 tggaactcat ccaggacact taaaaagaag atggtacttt tatcttcatt acctgaagct
     1081 tgttttggct ggaatttttc tacatccacc ccgttgggta ttacttctat tttatcccgg
     1141 tatttagcca gatgagatga ggattgcagg tagccgggct gggttatgat gatcttagct
     1201 gcagttttga gcacgtagtt taagcctact gagttatata tacgggctac cagacttgcc
     1261 aatccctgac ctattatatc gttatg
//
LOCUS       DAOQVW010000514         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_150593_length_1285_cov_2.426016, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000514 DAOQVW010000000
VERSION     DAOQVW010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150593_length_1285_cov_2.426016"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1158)
                     /locus_tag="PKK85_09135"
     CDS             complement(256..1158)
                     /locus_tag="PKK85_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNS26217.1"
                     /translation="MARKKIEDLVVNLEFPRSINTTFAVLHKSLKIASSGRHYLALSL
                     ADKTGWMDGRMFPDSGVEDIYNSIPTGTICRVEGKVNEFPPGSGKMNIVIKSLQELCE
                     EEYQLEDFIATSPHDCEKQVEVIRGVIRGMESPELKGLLRSFFCDQEFTAKFYQAPAA
                     MIHHHNYLGGLLDHSVEVLLICERLCELFPQLDRDLLLTGALLHDVGKIRTYQYHKVK
                     IEMSRESVLLDHLYLSADMVRERMESLNFPEELYQQVLHLILSHHGKVSQGWGSSVNP
                     KLPEAMALHHADHLDARVKEMLQK"
BASE COUNT          318 a          343 c          295 g          329 t
ORIGIN      
        1 ggaagctcct ctctggtctg gatcactccc tatgggtcag aggcacacat gtgcttcctt
       61 atcttcccca gtttatttat cattccccat cagccagcgg tgatcccaga taattcgctt
      121 cccagttacc aggataaaag tagcttatgc catccatgta ccgggggaag gtggtggacg
      181 gcacaatagt aggccaaaaa gatatcctgg gctggactga tatcacaatt catgtgcaca
      241 ccctacctta gccagctact tctgcagcat ctccttcacc cgggcatcca ggtgatcagc
      301 gtggtgcagg gccatggcct ctggtaactt gggattaaca gaagaacccc agccctgact
      361 taccttacca tggtggctta ggatgaggtg cagaacctgt tgataaagtt cctccgggaa
      421 gttaagactc tccattcttt ccctgaccat atcagccgag aggtacagat gatccaggag
      481 gacgctctcc cgggacattt cgatcttaac cttgtggtac tggtaggtcc tgattttacc
      541 cacatcatgg agtaaagccc cggttaagag caggtcccgg tccagttggg ggaataactc
      601 acataacctt tcacagatta agagcacctc aacagagtgg tccagaagtc ctcccagata
      661 attatggtgg tggatcatgg cagccggggc ctggtagaac ttggcggtga attcctggtc
      721 acagaagaag gatcttaaca ggcccttcag ctccgggctt tccatacccc ggataacccc
      781 ccggataacc tccacttgct tttcacagtc atggggggag gtggcgatga aatcctccag
      841 ttggtattct tcttcacaca gctcctgcaa tgatttgatg acgatgttca tcttcccgga
      901 tccaggtggg aactcattaa ctttcccctc caccctgcag atggtgccgg tgggtatgct
      961 gttgtatata tcctccaccc cactatccgg gaacatccgg ccatccatcc atcctgtctt
     1021 atcagcaagg gacaaggcca gatagtggcg tccactggag gcgattttca ggctcttgtg
     1081 taacacggcg aaggtggtgt tgatactccg gggaaactcc aggttcacca ccaggtcttc
     1141 aatttttttc cgggccatat ccaaattccc caccaagtaa ggttcttaac attctctcta
     1201 ttatccctgg tccttaaggc tttctaaagc caccaatcaa taagctaata taattaaatc
     1261 aaaaaagaat agaataatga aaatt
//
LOCUS       DAOQVW010000515         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_151399_length_1281_cov_2.502447, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000515 DAOQVW010000000
VERSION     DAOQVW010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151399_length_1281_cov_2.502447"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PKK85_09140"
     CDS             complement(<1..281)
                     /locus_tag="PKK85_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953898.1"
                     /note="catalyzes the formation of 3-phosphonooxypyruvate
                     from 3-phospho-D-glycerate in serine biosynthesis; can
                     also reduce alpha ketoglutarate to form
                     2-hydroxyglutarate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNS26218.1"
                     /translation="MKVLIADQINEKGIEELKEVAEVVVRTTITPEELVNDINDFEAI
                     VVRSRTKVTREVIEAAPKLRIIARAGVGVDNVDVEAATERGVMVVNAPES"
     gene            579..917
                     /locus_tag="PKK85_09145"
     CDS             579..917
                     /locus_tag="PKK85_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016358020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNS26219.1"
                     /translation="MVSVEEFVIVSSNYVPGYEILETKGFVYGLTVRSRGMGGQIGAG
                     IRSMFGGEIKEYVHMMEETREEALNRAIQHAQDMGANAIISARYDSNDISDIMQEILV
                     YGTAVVVQKE"
     gene            929..>1281
                     /locus_tag="PKK85_09150"
     CDS             929..>1281
                     /locus_tag="PKK85_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26220.1"
                     /translation="MFEGCLKLNNVTLPRTLLGTSPFIGAEQFGHRARLYQLDLYNHP
                     KKMLQIIRTSYDEGVRGMQLLPYPPVIQALEMAREDGMEIDVIGTVRPDQEKEDIKLL
                     ADLNAKAMLLHAITT"
BASE COUNT          371 a          261 c          291 g          358 t
ORIGIN      
        1 gattccgggg cattaaccac catcaccccc ctttctgtag cagcctccac atctacgtta
       61 tccaccccta caccggcacg agcgattatt cggagtttag gggcggcttc aatgacttcc
      121 cgggtgactt tggttctgct cctcaccact atggcttcaa aatcgttaat atcattaaca
      181 agctcttcag gagtgattgt ggtgcggact actacttcag ccacctcttt cagttcctct
      241 atcccttttt cattgatttg atcagcgata agtaccttca tgttatccat cttattcacc
      301 agttttctgt ggtttcatct catagttatt ggagtgggaa tgtttaaatc tttgctgacc
      361 attgagggtt agggggtctg aaaaagtaga acaattcaag agaaaataaa caatccacac
      421 tacatattgg ctttaataac tgctcatact cattttaaag taagttaagg tagataaatc
      481 cttattgggg atgagatatt atttataaag ttaaatgatt ggacaatata tatatggtga
      541 attacggata aagtactgga gatttgtgag gtgttcagat ggtttcagta gaagaatttg
      601 taattgtgag ttccaactac gtgcctggat acgaaatcct tgaaactaag ggttttgttt
      661 atggtcttac cgtacggagt cggggtatgg gtggacagat aggagcagga attcgttcca
      721 tgtttggagg agagatcaaa gagtacgtgc acatgatgga agaaacccgg gaggaagctc
      781 taaacagagc cattcaacat gcccaggata tgggggccaa tgccatcatc agcgcccgat
      841 atgattccaa tgatatatca gatataatgc aggaaatact ggtttacgga actgccgttg
      901 tggttcagaa agaataagtg agaatcaaat gtttgagggc tgtctaaaac ttaacaacgt
      961 tacattaccc cgtactctgc tgggaacatc acccttcatt ggagcagaac agtttggaca
     1021 tcgagcacgt ctgtatcagc tcgatcttta taaccatccc aaaaaaatgc tccagatcat
     1081 tagaaccagt tacgatgagg gtgtgagggg gatgcagctt ttaccctacc ccccagtgat
     1141 tcaggccttg gaaatggccc gggaagatgg catggaaatt gatgtaatcg gaacagtacg
     1201 tccagatcag gaaaaggagg acattaaact cctggcagat ttaaatgcca aggccatgct
     1261 actccacgcc attactacag a
//
LOCUS       DAOQVW010000516         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_151714_length_1279_cov_3.944444, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000516 DAOQVW010000000
VERSION     DAOQVW010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151714_length_1279_cov_3.944444"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKK85_09155"
     CDS             <1..612
                     /locus_tag="PKK85_09155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS26221.1"
                     /translation="GINPQKVILTSNAVDMNRFSPYNSPPSDLHLVPHKTTLLFVGNL
                     VPQKGVKYLLEGKKILDDHLKHDGTGREYELLIVGDGPLRKALERKVQEDDIKDVVFL
                     GERRDVEKIMPAVDLFVLPSISEGFPISILESLASGVPVVATNVGGVVEIESGSIVQL
                     VEPGQPEALTQAIMQMEEYISQKVVKKRARETARKYAQIKIPY"
     gene            649..1122
                     /gene="moaC"
                     /locus_tag="PKK85_09160"
     CDS             649..1122
                     /gene="moaC"
                     /locus_tag="PKK85_09160"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNS26222.1"
                     /translation="MENRKFTHLSQEGVQMVEVGEKPLVKRTALASGKIKLEPITIEL
                     IVNEEIKKGNVLTTAQIAAIQAVKSTHHLIPLCHPLSITGINVEFDVQKEHITVEVEV
                     RTTGQTGVEMEAITGVSVALLTIWDMVKSVEKDEDGQYPHTQITDIKVLRKEKLA"
BASE COUNT          416 a          267 c          291 g          305 t
ORIGIN      
        1 ggtataaacc cccaaaaagt aatcttaact tccaatgcag tggatatgaa ccgtttttct
       61 ccctacaact cccctccatc tgatttgcac ttggttcctc ataaaaccac cctccttttt
      121 gtaggcaacc tagtacccca aaagggtgtt aagtacctcc tggagggtaa aaaaattctg
      181 gatgatcatc tgaagcatga tggtacaggc cgtgaatatg aactcctaat cgttggtgat
      241 ggccccctga gaaaggcact agaaagaaag gtgcaggaag atgatattaa ggatgtggtc
      301 tttctgggtg aacgtcgtga cgtggaaaag atcatgcctg cagtggatct gttcgtcctc
      361 cccagtattt ctgaaggttt tcccatctcc attctcgaat cactagcatc aggcgtgcct
      421 gttgtggcca ccaatgtggg aggagtggtg gaaatagaat caggatccat agtccagttg
      481 gtggaacccg gccaaccaga agctttaacc caggccatta tgcaaatgga ggaatatatc
      541 tcccagaaag tagttaaaaa acgggcccgt gaaacagcca gaaaatatgc acagataaag
      601 ataccttatt aatggtaaat atatcatatt tgattacgag ttgattccat ggaaaacagg
      661 aaatttaccc acctttccca ggagggagtg caaatggtgg aagtaggaga aaaacccttg
      721 gtcaaaagaa ccgccctggc cagtggaaag ataaaactgg agccaattac catagaactc
      781 attgttaatg aggaaattaa gaaagggaac gtgttaacta ctgctcagat tgcagcgatc
      841 caggctgtta agtccaccca ccatttaata ccactctgcc atcccctatc catcactggc
      901 attaatgtgg aattcgatgt ccaaaaggag cacataactg tggaggtgga ggtccgcacc
      961 actggccaga caggagtaga gatggaagcc ataactggag tgagcgtggc attgctaacc
     1021 atctgggata tggtgaagag tgtggagaaa gatgaggatg gccaatatcc gcacacacag
     1081 attactgata taaaagtact cagaaaggaa aaattagctt gaaattttca cattttaaag
     1141 tttataatgg actaaaaaaa gctaaaacaa attgagatct ggttgtatga aatatataga
     1201 ccctaaaatc agagatatca tcaaaaagtg ctatccctac atcgaagacc ttaacccagc
     1261 ccagcaagca gcagtggag
//
LOCUS       DAOQVW010000517         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_151740_length_1279_cov_3.133987, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000517 DAOQVW010000000
VERSION     DAOQVW010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151740_length_1279_cov_3.133987"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PKK85_09165"
     CDS             <1..518
                     /locus_tag="PKK85_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS26223.1"
                     /translation="VEEALDFFKNIPRISKKLKTLDDVGLGYIKLGQPATTLSGGEAQ
                     RVKLAKELSRQSTGRTLYILDEPTTGLHFADIKKLLQVLVRLRDAGNTVMVIEHNLDV
                     IKTADHIIDLGPEGGDEGGRVVAQGTPEEIVASKTYTGKFLETVLGECRELSSSVSTG
                     AEAGKEGGLNK"
     gene            863..>1279
                     /locus_tag="PKK85_09170"
     CDS             863..>1279
                     /locus_tag="PKK85_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNS26224.1"
                     /translation="MNVPMMVLQIPIIREFGGPSYEDLFRLLILIILVIVVALLAERI
                     EKVRKLNQLNRDLENSAIQLHEANEELEAFAYSVSHDLRVPLRAIDGFSRILMEDYGD
                     SLDEEGNRLISIVRENTEKMGDLIDDILLLSRVGRQK"
BASE COUNT          394 a          256 c          286 g          343 t
ORIGIN      
        1 ctgtggagga ggccctggac ttctttaaaa atatcccccg cataagtaag aagcttaaaa
       61 ccctggatga tgtgggtctg ggttatatca agctggggca gccggcaacc accctatcag
      121 ggggggaggc ccaaagagtc aaactggcca aggaactgag ccgtcagagc accggaagaa
      181 ccctctacat tctggatgag cccaccaccg gcctccactt cgcagatata aagaagctat
      241 tacaggtact ggtaagacta agagatgctg gtaacacagt catggtcata gaacacaacc
      301 tggacgttat aaaaactgcc gatcacatca tcgatctggg acctgaagga ggagatgaag
      361 ggggcagggt agtggctcag ggcacccccg aagaaatcgt tgcaagtaag acctacactg
      421 gaaaattcct ggaaactgtc ttgggagagt gtcgagaatt atcttcgagt gtgagcactg
      481 gagctgaagc aggaaaagag ggcggcctca acaaataata aaaaaaagcc atattctcat
      541 ttcttttttt ctccctttct ataaccaccc ccagtttcta tatcacttgt tctttaaaac
      601 taaaaatctg attttgaatt atcaaacttt ctattcctta tacatatctt taattctatt
      661 ccatgaattt tttgcacaaa aaattggtga aaccattttt atgaatttat ctcgatgatc
      721 attcccatca tcctcagcta atagaaaaag ccaattacaa tgaaaggttt attaaattgt
      781 aatgctgcaa taaattataa tgatgatagt aaatcataac ttcatttagg aatgaggggg
      841 acgttgagta gtgtttagta tcatgaatgt gccaatgatg gttttacaaa tcccaataat
      901 tagggagttt ggagggcctt cctatgaaga cctttttcga cttcttatcc tgatcattct
      961 agtaattgtg gttgctcttt tagcggagag aatagaaaag gtgcggaaac taaatcaact
     1021 caatcgggac ttggaaaaca gtgccattca actacatgaa gcaaacgagg aactggaagc
     1081 ctttgcctat tcagtttccc atgacctgag agttcctctc agagctattg acgggttttc
     1141 caggatatta atggaggatt acggagattc gcttgatgaa gaagggaacc gcctcataag
     1201 cattgtccgt gaaaacacgg aaaagatggg tgatctcatt gatgatatcc tgcttctatc
     1261 ccgggtgggg cgtcaaaag
//
LOCUS       DAOQVW010000518         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_151929_length_1278_cov_4.098937, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000518 DAOQVW010000000
VERSION     DAOQVW010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151929_length_1278_cov_4.098937"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PKK85_09175"
     CDS             complement(<1..531)
                     /locus_tag="PKK85_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNS26225.1"
                     /translation="MQSLGDWRRTHYSRQVKPDMDSEKVTLMGWIHEIRDLGGIIFLL
                     LRDRDGVTQITAPSKKVSKELIEEIRKLRKESVVAVKGTVQESGKAPGGVEIIPEEIE
                     LLNQSQQPLPLDPTEKVRAEIDTRLDSRFLDLRKHSVNSIFKIKSRMLHSVRLFLEEQ
                     EFIEINTPKLVASATEG"
     gene            complement(681..>1278)
                     /gene="hisD"
                     /locus_tag="PKK85_09180"
     CDS             complement(681..>1278)
                     /gene="hisD"
                     /locus_tag="PKK85_09180"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNS26226.1"
                     /translation="PAGPSEVLVLADENANPEYIALDLLAQAEHDPDAASVLVTTSPE
                     LAKEVQQRLLQELPRMKRREIIEVALAKYGHIIVAPTMEEATQFTNHYAPEHLIIMTQ
                     NPEQELAGITNAGSIFLGELTPVAAGDYGSGTNHVLPTSFCARMYSGLSTESFLKKPS
                     FQKLTRDGLVDLKKTVVTLAEYEGLYAHAESLKRRLAR"
BASE COUNT          277 a          322 c          311 g          368 t
ORIGIN      
        1 cccctcagtg gcggaagcca ccagtttagg ggtgttgatt tcgataaatt cctgttcctc
       61 caggaataaa cgaacggaat ggagcatcct gctttttatc ttaaatatgg agttcacact
      121 gtgtttgcgc agatccagga aacgggagtc cagtcgggta tcgatctctg cccgtacctt
      181 ttccgtagga tccaggggca ggggttgctg ggactggttt agcagttcaa tctcttcagg
      241 aattatctcc accccaccag gggccttacc agattcctgg actgtgccct tcactgccac
      301 tacagactct ttcctgagtt tcctaatctc ttcaatgagt tcctttgata ccttcttgct
      361 gggagcggtt atctgggtaa caccatcccg gtcccttaaa agcaggaata ttattcctcc
      421 caggtcacgg atttcatgta tccatcccat gagagttact ttttcactgt ccatatctgg
      481 cttaacttgc cgtgaataat gggttcttct ccagtctcct aatgattgca tcacagtaat
      541 tcacctcggt aacgttaaaa tatttactaa aaggggattc acttcatccg aaatgaaagc
      601 ttgtctaatc actataaatc tagagtccgg acttataaaa gtgtcatgta ccattctagc
      661 atcctcgtca ccccagccta tcaccgggct aatctccgtt ttaaggattc agcgtgggca
      721 tataaacctt cgtattcggc cagggtcact acagtttttt ttaggtcaac cagtccgtcc
      781 ctggtgagtt tctggaagct tggctttttc aggaaggatt cagtggataa acccgagtac
      841 atacgggcac aaaatgaggt ggggagcacg tggttggttc ctgatccata gtcccccgct
      901 gccacggggg ttagttcacc caggaatatg gaacccgcgt ttgtgatccc agccagttcc
      961 tgttccgggt tctgggtcat gatgatcagg tgctctggtg cgtagtggtt ggtgaactgg
     1021 gtagcctctt ccatggtggg tgctactatt atatggccat actttgccag ggccacctct
     1081 atgatttccc gtcttttcat gcggggcagt tcctgtagca accgttgctg gacttccttt
     1141 gccagttccg gtgaggtggt taccagaacc gaagcagcat ccgggtcatg ctcggcctgg
     1201 gctaaaaggt ccagggcaat gtactcggga ttggcgtttt catcagccaa taccagcacc
     1261 tctgagggtc cagctggg
//
LOCUS       DAOQVW010000519         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_152610_length_1275_cov_2.503279, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000519 DAOQVW010000000
VERSION     DAOQVW010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152610_length_1275_cov_2.503279"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKK85_09185"
     CDS             <1..229
                     /locus_tag="PKK85_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26227.1"
                     /translation="YDKYLSKGPLGDITGNTQMQGSPTQNAHEDTMNDLFTDTAGGIV
                     GALVGVLFIRRAEKKGEHWEVVDKIEKMGQD"
     gene            complement(240..407)
                     /locus_tag="PKK85_09190"
     CDS             complement(240..407)
                     /locus_tag="PKK85_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26228.1"
                     /translation="MANIIIAKNMPPPQIHKRWIFLLNHCEGYGGLEPVATQAMDTTF
                     NDKTLVRTFLR"
     gene            793..>1275
                     /locus_tag="PKK85_09195"
     CDS             793..>1275
                     /locus_tag="PKK85_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNS26229.1"
                     /translation="MKENEPKALKYMQKVQRGMTRWRDKVAKTEFNQENFKVEEVEVI
                     IPSMAREFDGYRLGNISDIHLGQWITPEYLKGVMHLVNKEKPDSVTITGDFVSYVLDD
                     VLDDLESALSLLKPKDKSFAVLGNHDHWLGAAQIRSILQASNIIDVSNDVHSLFRKKS
                     T"
BASE COUNT          399 a          223 c          281 g          372 t
ORIGIN      
        1 atatgataaa tatttgtcta aaggtccttt aggtgatata acaggaaata ctcagatgca
       61 gggctcaccc acccagaatg cccatgaaga tacaatgaac gacctattca cagatactgc
      121 cggtggtatc gtaggtgcct tagtgggggt gctgtttatt aggagggctg aaaagaaagg
      181 cgagcattgg gaagtagttg ataaaataga aaagatgggt caagattaag cccctgatct
      241 catcttaaaa aagtccgaac tagtgtttta tcattgaatg tagtgtccat tgcttgggtg
      301 gcgacgggct ctaagcctcc ataaccttcg cagtggttta gaagaaagat ccagcgtttg
      361 tgtatttgag gggggggcat gttcttggca atgataatgt ttgccatgaa ttaatcacca
      421 tcattttggt catctactcc cccgacagtt aaaacagttt ccagcgtgaa ttggccggaa
      481 aactagtttc tttcttcttc tgaaaatcat caccatctgg tagactgaaa tctccttatg
      541 cttaccaaag gggaatcaaa tcatttctaa gttaaatcgc tgaatataaa acatagccat
      601 ttatggcata tttgctgttt atcttgatgt atatctatta aaggacatga ttataattgg
      661 gtttaaatct aaattcagta attaatgtaa atgaaatggt ctttgtgaga ttgttgggtg
      721 aggtattgtg atttgtcctt gaattatgtc actatataac gctccgaaaa cagaaataaa
      781 gctctggaaa ttatgaaaga aaatgaacct aaggctctaa aatacatgca gaaagtgcag
      841 cggggaatga ctcgttggcg agataaagta gccaaaaccg aatttaatca agaaaatttc
      901 aaggtggagg aagttgaagt aatcataccc tctatggccc gcgaatttga tggttatcga
      961 ctgggtaata tttctgatat tcatttaggg caatggataa ctcccgaata cttgaaagga
     1021 gttatgcatc tggttaacaa ggaaaaaccg gattctgtaa ccataactgg agattttgtc
     1081 tcctacgttc tggatgatgt cttggatgat ctggaatctg ccctcagcct tttaaaacca
     1141 aaagataaat ctttcgctgt tttaggaaat catgatcact ggctaggcgc agcgcagata
     1201 cgaagtatac tccaggccag taacatcatc gatgttagca acgatgtgca cagcttattc
     1261 cggaaaaaat ctacc
//
LOCUS       DAOQVW010000520         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_153009_length_1273_cov_2.512315, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000520 DAOQVW010000000
VERSION     DAOQVW010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153009_length_1273_cov_2.512315"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKK85_09200"
     CDS             <1..569
                     /locus_tag="PKK85_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNS26230.1"
                     /translation="SILLGILAVPAALFFVVLLKISGRIMEPLQEKPILRSTLGGLFL
                     GLLAVVLPTTIGLGSNQMPLVTYQAAEIGVVLLLIFALAKMLALSSALSSGFIGGPIF
                     PLLFVGSCLGAALNQIFPQLPLGLALGCMIVAIPAAIVPIPLSLAAIVIIVIGLPPVN
                     ILPILVAALVSFTITRGLIMGGKELEEA"
     gene            762..844
                     /locus_tag="PKK85_09205"
     tRNA            762..844
                     /locus_tag="PKK85_09205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:796..798,aa:Leu,seq:cag)
BASE COUNT          267 a          356 c          291 g          359 t
ORIGIN      
        1 tgagcatcct cttaggtatc ctcgctgttc cagctgccct gttttttgtg gtccttttaa
       61 aaatctcggg gagaatcatg gagccactcc aggagaagcc tattctccgc tctaccctgg
      121 gcggtctctt cctgggcctg ctggcggtgg tgctccccac caccattgga ctgggaagca
      181 atcaaatgcc actggtcacc taccaggccg cggagatcgg tgttgtactc cttctaattt
      241 tcgcattggc caagatgctg gccttgagca gtgccctgag ttctggattt attggcggcc
      301 ccatcttccc cctcttattt gttggctcct gcttaggcgc agccctcaac cagatcttcc
      361 cccaactccc cctaggcttg gcactgggct gtatgatcgt ggccatacct gctgccatcg
      421 tacccatccc attgtccctg gcagccatcg tcatcatcgt cattggcctc cccccagtga
      481 acatcctccc cattctggtg gcggccctgg tttccttcac catcaccagg ggacttatca
      541 tgggtggtaa agaattagaa gaagcttagc tgggttattg taaaccaata gaaatctaca
      601 tatatccatt acttaatcct agacctgtgg atacagggtt ctagttctgt tcatatagaa
      661 ccttccatcc ccagagcttg ctatggttat ggttttactg gcagtggatt gggaaaagaa
      721 aaagctatat atgtatacga tcccacaaac aatggtacca ggcaggggtt cccgagcggt
      781 caaaggggat aggttcaggg cctattggtg taggccttcg agggttcgaa tccctccccc
      841 tgcacttatt ttttacagta acagggcacg ttatcttcag aatccacaga gactgctagc
      901 tgacccaggg tagggcactt acctctaact aggtttatat tacttgtcta tgctttcctg
      961 gcagggctct agtgggacca tgaatctgtg cataattctg tggctggttt cattcatctc
     1021 caggactggg ttccttccat tttccccggc accagggaca ttttatctag attcagtgat
     1081 ttcatagtac tataatgaac gaattgctca tccctaaacc tgggcgtact cctctttaag
     1141 cttcaaccag tccaccttcc ctatgagggt ggtgggcagc tcctccctga tctcgaactc
     1201 ccggggaaca ctccaggtag ccaggtactc ccggcacagc gacatgatct cacttttgat
     1261 cctatccgta tcg
//
LOCUS       DAOQVW010000521         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_154428_length_1266_cov_2.538398, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000521 DAOQVW010000000
VERSION     DAOQVW010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154428_length_1266_cov_2.538398"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..764)
                     /locus_tag="PKK85_09210"
     CDS             complement(180..764)
                     /locus_tag="PKK85_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNS26231.1"
                     /translation="MVKVMGIVGSPRKDGNTHKLVEAALYTAQSAGATVELLTLHDKE
                     MEPCIACDICRATGECAIYDDVPGVLERMVESDGIIIGSPVYFGNVTSQLKILMDRSR
                     PLRRDFQLKNKVCGALSVGASRNGGQETTIQAIHHYLLIQDAIVVGDGEPLAHYGGTG
                     VGGPVGDVDGDEAGLETSQNLGRRVAELARLINP"
     gene            872..>1266
                     /locus_tag="PKK85_09215"
     CDS             872..>1266
                     /locus_tag="PKK85_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26232.1"
                     /translation="MMTENFYTAFTPSARVLDALAYYFQEERMGDTTARRRAQNYLRD
                     FMVETGDGSYTLPSQDPSENMHSTHGAATEARMKYAIPSGLGSKSQVRVLDLCTGLGY
                     NAAALLEQQRGRVELDLVEYSQEALAAAAG"
BASE COUNT          269 a          401 c          327 g          269 t
ORIGIN      
        1 cgggccacct cccgggtgtg gtcctggcta tccccatcca ccaccaggac ctcgtactca
       61 tcagggggga cgtcctggtc caccagggat tccagggtcc gggccaggta ctcctcctcg
      121 ttgcgggcca cgatacccac cgttaccttg atggacatgt tcaaataccc ttccaccact
      181 tacgggttta ttaaccgggc caactccgcc acccggcgtc ccaggttctg gctggtttcc
      241 aagccagctt catccccatc tacatccccc actggtcccc ccacaccggt tccaccgtaa
      301 tgggccaggg gctccccatc acccaccact atggcgtcct ggatgaggag gtagtggtgg
      361 atggcctgga tggtggtttc ctgaccccca ttccgggagg cacccacgga tagggccccg
      421 cataccttgt ttttcagttg gaaatccctt cggagggggc ggctgcggtc catcagtatc
      481 ttcaactggc tggtgacgtt gccaaagtac actggactgc caatgatgat gccatcagac
      541 tccaccatcc tctccagcac ccctggcaca tcatcgtaga tggcgcactc cccagtagcc
      601 cggcagatgt cacaggcaat acagggctcc atctccttat catgcaacgt taaaagctct
      661 acagtggcac ctgcactctg ggcagtgtac agagcagcct ccaccagttt atgggtgttc
      721 ccatccttcc gggggctgcc cactattccc atgaccttaa ccattcatca tcacatccta
      781 agattacttt tagtttataa ttttattaag gggtttatat atagagagac tcaggatcag
      841 tggatcccag cacgtattaa tggtactgat tttgatgaca gaaaacttct acactgcctt
      901 cacaccatca gcccgggtcc tggatgccct ggcatattac ttccaggagg aaaggatggg
      961 tgacaccacg gcccggagac gtgcccagaa ctatctcagg gatttcatgg tggagacagg
     1021 ggacggatcc tacactctcc cctcccagga cccctcagag aatatgcaca gcacccacgg
     1081 ggccgccacc gaggcccgga tgaaatacgc cataccctcc gggttaggat caaagagcca
     1141 ggtacgggtc ctggacttgt gcacgggtct gggctacaac gccgcagccc tcctggaaca
     1201 gcagagggga agggtggaac tggacctggt ggagtactcc caggaggccc tggccgcggc
     1261 cgccgg
//
LOCUS       DAOQVW010000522         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_155914_length_1259_cov_2.799003, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000522 DAOQVW010000000
VERSION     DAOQVW010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155914_length_1259_cov_2.799003"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1196)
                     /gene="porA"
                     /locus_tag="PKK85_09220"
     CDS             complement(48..1196)
                     /gene="porA"
                     /locus_tag="PKK85_09220"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorA"
                     /protein_id="HNS26233.1"
                     /translation="MVLKVMTSNRAVAEAVKMAKPDVVPVYPITPQTTISEYLAKFTA
                     DGDLKTEYIRVESEHSAISAALGASSAGVRVFTATSSQGLALMHEILFATAGMRSPVV
                     LVDANRALSAPLSIWNDQQDTISERDSGWLQIYAENAQEALDAVLIAYRVAEDPEVLL
                     PCMVCIDGYFLTHTVEPLDVPSQEQVDQFLPPYKPYAFLDPEKPMSIGTFTDPEYYME
                     ARYDIELAMEKSKEVMAQANQEFREIFGREYDLIEKYRCDDAEIIIVAMGSVCGTIKD
                     VIDSLREEGEKVGLLKIRVYRPFPWEDIKEVLEKASRVAVLDKNISFGIGGVLYNEIK
                     AKTNADAYGFIAGLGGRDITPEYVKEIVAKAKNPTREVEWIGLKKEEV"
BASE COUNT          280 a          324 c          279 g          376 t
ORIGIN      
        1 catcctcgat gtccaggggc caggaattct tctgtactga tttccattta aacttcctcc
       61 tttttaagtc ctatccattc aacctcacgg gtggggtttt tagctttagc aacaatctct
      121 ttgacgtatt caggggttat atcacgtcct cccaatccag cgataaaccc atatgcatca
      181 gcattggttt tggccttaat ctcgttgtag agaactcctc ctatcccgaa ggagatgttt
      241 ttgtccagaa ccgcaacccg ggatgctttc tccagcacct ccttaatatc ctcccagggg
      301 aagggacgat atacacggat ttttaggagc cctacttttt ctccttcttc tctaagtgaa
      361 tctataacat ctttaattgt accacataca gatcccatag ctacgataat gatttctgca
      421 tcatcacacc gatacttttc gattaaatca tattctcttc cgaatatttc tctaaactcc
      481 tggttagcct gggccataac ctctttggac ttttccatgg ctagttctat gtcgtatcgt
      541 gcttccatgt agtactctgg atcggtgaag gtaccgatgg acatgggctt ttccgggtcc
      601 aggaaggcgt acggcttgta gggtggtaag aattggtcta cctgttcctg gctgggcaca
      661 tccaggggtt ccacggtgtg ggttaggaag tatccgtcaa tacagaccat gcagggcagc
      721 aagacttccg ggtcctcagc gacgcgatag gctatcaaca cagcatctaa ggcttcctga
      781 gcattttctg cgtatatctg cagccatccg gagtctcgtt ctgatatggt gtcctgttgg
      841 tcattccaga tgcttaaagg ggcggacaat gcacggttgg catccaccag aaccactgga
      901 cttctcatac ccgcggtggc aaagagtatc tcgtgcatca gtgccagtcc ctgtgaggag
      961 gttgcggtga aaaccctgac accggcactc gatgctccta gagcagcact tatagcactg
     1021 tgttctgatt ctaccctgat atattcagtt tttaaatctc catctgccgt gaattttgcg
     1081 aggtattcgg atatggtggt ctgaggggtg atggggtaaa caggcaccac atctggtttc
     1141 gccattttca ctgcttcggc cacggcccgg ttggaagtca taacttttaa caccatttct
     1201 atcccctctc cattttaata gcttttactg ggcattcttc agcgcaaatc ccgcagcct
//
LOCUS       DAOQVW010000523         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_156242_length_1258_cov_1.743973, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000523 DAOQVW010000000
VERSION     DAOQVW010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156242_length_1258_cov_1.743973"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PKK85_09225"
     CDS             complement(<1..180)
                     /locus_tag="PKK85_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNS26234.1"
                     /translation="MKTLISNLRGQCLFDASVKIQADGIISLHEGKYHRSEIDSFLKG
                     GEILIETDDPKEACQR"
     gene            382..999
                     /locus_tag="PKK85_09230"
     CDS             382..999
                     /locus_tag="PKK85_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tributyrin esterase"
                     /protein_id="HNS26235.1"
                     /translation="MSEEKNNIILEKTGKLDSIVVGLGKEANVTLEGDVGDFVGALNN
                     GARIEIQGNAGRYVGDNMTTGEIVVKGSAQDGVGFGTYNGTIVVYGDAGNGVGQLNKG
                     GTILIDGNIGDLTGLYMLSGDIIVTGDAGADTGDWMIGGTIYIGGNYETGTNAEVHEL
                     DPSDKRKLAELFKKYEIETQIEGFIKLKPKDLRPFYGNHNEEVTV"
     gene            996..>1258
                     /locus_tag="PKK85_09235"
     CDS             996..>1258
                     /locus_tag="PKK85_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS26236.1"
                     /translation="MKQILLTDPEKCDGCNDCIQACEYVNNESSIFLHKMDSGYHAIV
                     CQQCINPSCIKGCFRDAIYREDGVVKIDQDRCIGCRLCMLMCP"
BASE COUNT          405 a          246 c          293 g          314 t
ORIGIN      
        1 ccgttggcag gcttctttag ggtcatcggt ttcaattaga atttccccac ccttcaggaa
       61 tgagtctatt tcggatctat gatattttcc ctcatgtaaa cttattatcc catctgcctg
      121 tattttaacc gaggcatcga agagacattg ccccctcaaa ttactgatta aagtcttcat
      181 agttggatca ccataaatac tctgtctcac ttcaatatat aattgtatac ctggaaagtt
      241 gtatggagaa caacctttaa atactgaact cttcctacca aaattagaga ttaaactgga
      301 gggatacaca cgaccatatc agaaaccatt aacgcaaaag ctttttcaac acgccgattg
      361 aacacacagg taaggaaagc gttgtctgag gaaaaaaata acatcatact ggaaaaaaca
      421 ggtaagctag actctatagt tgtgggatta ggtaaagagg caaatgtaac tttggaagga
      481 gacgttggag attttgtagg cgcccttaac aacggggcac gtatagaaat tcagggaaac
      541 gcaggacgat acgtgggaga caacatgacc actggagaga tcgtagtaaa aggttcagcc
      601 caagacggag tgggcttcgg cacatataac ggtaccatag ttgtttatgg tgatgccggc
      661 aacggtgttg gtcagctaaa taagggcgga accatcctta ttgatggaaa tattggtgat
      721 ctgacaggcc tctacatgct cagtggcgac ataatagtca ctggagatgc cggtgcagat
      781 accggggact ggatgatagg cggcaccatc tacataggtg gtaattacga aactggaacc
      841 aatgcagagg tccatgaatt ggatccttca gataagagaa aattggctga actatttaaa
      901 aaatatgaaa ttgagactca aattgaaggc ttcataaagc tgaaacccaa ggatctaagg
      961 ccgttctatg gaaaccataa cgaggaggtg acagtatgaa acagatcctc ttaactgatc
     1021 cagaaaaatg cgacggatgc aacgactgta tccaggcctg tgaatatgta aacaatgaaa
     1081 gcagcatatt cctgcataaa atggacagtg gataccatgc catagtctgt cagcagtgta
     1141 taaatccctc atgtattaaa ggatgcttcc gggatgccat ctaccgggaa gatggagtgg
     1201 tgaaaataga ccaggaccgc tgcatcggtt gcagattatg catgctcatg tgtcctat
//
LOCUS       DAOQVW010000524         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_157139_length_1253_cov_3.419032, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000524 DAOQVW010000000
VERSION     DAOQVW010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157139_length_1253_cov_3.419032"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKK85_09240"
     CDS             complement(<1..233)
                     /locus_tag="PKK85_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNS26237.1"
                     /translation="MRKSRFEKGMENLKVMNPQSFQTLKDSLTDVAPDLARYVVEFPY
                     GDIYNRPGLDLKTRELVTIASITTMGGAATQLK"
     gene            563..1221
                     /locus_tag="PKK85_09245"
                     /pseudo
     CDS             563..1221
                     /locus_tag="PKK85_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262155.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
BASE COUNT          371 a          244 c          275 g          363 t
ORIGIN      
        1 cttttcaact gtgtcgctgc ccctcccatg gtggtgatgg aggctatggt aaccagttct
       61 ctggttttca aatccagccc tggacggtta taaatgtcac cgtaggggaa ttcaacaacg
      121 taccgagcca gatcgggtgc tacatcagtt agactgtcct tcagtgtctg aaatgattgg
      181 ggattcataa ctttaagatt ttccatgcct ttctcaaatc tgctcttcct cattttctca
      241 ccaaaaatat ctcttttctg taggtagaag tattttttaa aaactaatag tattaatact
      301 aaatttagga taatctaagt gaagtacact gtgaagttgg tgaagataaa aattcacaag
      361 tttcttctac catgagcttg gcattagtca tttcactgct tcaacaccta aacacaatct
      421 gcaggggaag taaagtacac tttggatagg tgctccaagt gccagtgacg cccacaaatc
      481 agaaggccca taggatacaa caaaccctgt atggagcatc atcaatatca aagaaactct
      541 ataaggaata ttaatgattt aaatgaagcc tcgagttata ttaaatgctg ccatgacttt
      601 ggatggtaaa atagccacca aaaagggtag ttctgaaata tctggtgatg aagatctttt
      661 aagggttcat gtattgcgaa gggactgtga tgccatcatg gtgggcatta acacggtgtt
      721 agctgatgat cctcgcctta ccatccataa aattccttca aatccagctg atatcctata
      781 cgggtggtgg tggatagtca ggcccgaaca ccactctctt cacgggttct atctgaagat
      841 gcccccacca tcattgccgt gagtaaaaga gctccactgg atagattgga caaacttagt
      901 cagcaagcta agataatcat ttgtggtcaa gaaaaggtgg atctagaatg tttaatggat
      961 aatttaaaag accagggtat atgtactctc atgttagagg gcggatctgc actaaacttt
     1021 tctatgctct ctcatggtct ggttgatgag gtccgggtgt gtgtggcgcc tatgattgtg
     1081 ggtggaagtc aggccaagac actggtggat ggtgatggta tggattacat gtcccaggct
     1141 gtgaaactta acttaaaaaa atcatacccg ttgggtgaag atctcatcct ggaatatgaa
     1201 gtcatatcct caggggaatg acaacctaaa aaaaattgtt catcaatttt aag
//
LOCUS       DAOQVW010000525         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_157288_length_1253_cov_1.634391, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000525 DAOQVW010000000
VERSION     DAOQVW010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157288_length_1253_cov_1.634391"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKK85_09250"
     CDS             complement(<1..488)
                     /locus_tag="PKK85_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNS26238.1"
                     /translation="MNELGLNFLDLGDSDKLPIILIHGMALDQTMWTPQIPVLKEHYR
                     VLTYDIGGHGLSEVGDAQYTYKMFADDLKSLMDYLEMDQAVLCGLSMGGAIVLRAYDM
                     YPHRIKAMILSDARNEADSNETKYWRENSIESIKKDGLENFADEFVGNIFCQFQFQEA
                     SG"
     gene            complement(560..>1253)
                     /locus_tag="PKK85_09255"
     CDS             complement(560..>1253)
                     /locus_tag="PKK85_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNS26239.1"
                     /translation="IVNDFDVLYVVDTVSSLGGDDVFVDGYNIDICVTGSQKCLAAPP
                     GMAAITLSEDAWKVVDKTPTSTYYLDMKRYRKSGSGTPPETPYTPSVSLMYAMREALC
                     AVMEEGLESRVKRHQLAALATRNAIKALGLELFAQPEVSSNTVTAIKMPEGVTDKELR
                     GTMRDKYRIELAGGQDHIKGNVFRIGHMGNITEREIITTIAALEMTLKGLGLDICLGE
                     GVAAAEEAYLLD"
BASE COUNT          312 a          315 c          278 g          348 t
ORIGIN      
        1 cttccggatg cctcttgaaa ctggaactgg caaaatatgt tccctacaaa ttcatctgca
       61 aagttctcca gcccatcctt tttaatggat tcaatggaat tctctctcca gtacttggtt
      121 tcgttagaat cagcttcatt cctggcgtca gatagtatca ttgccttgat tctatgggga
      181 tacatgtcat aggcacgtag aactatggct cctcccatgg aaagtccgca caggacggcc
      241 tggtccatct ccaggtaatc cattaagctc ttaagatcat cggcgaacat tttataggtg
      301 tactgagcgt caccgacttc actgaggccg tggcccccta tatcgtaggt caacacacga
      361 taatgctctt taagaaccgg gatttgagga gtccacatgg tctggtccag ggccatgcca
      421 tggattaaaa ttatgggtag tttatcggaa tctcctaaat ccaggaaatt taaacccaac
      481 tcattcataa aaaaatcccc ccatatgatt tcttgattaa aatgaaaaaa atttattaaa
      541 aaatagtaaa aatagaagct taatcaagta gataagcctc ttctgctgca gccacacctt
      601 cacccaggca gatgtccagg ccaagccctt tgagggtcat ctccagggcg gctatggtgg
      661 tgatgatctc cctttcggtg atgttaccca tgtggcctat acggaacacg ttacctttaa
      721 tgtggtcctg gcctccggcc agctctatcc ggtatttatc ccgcatggta ccccgtaact
      781 ctttgtcagt tacaccttcc ggcatcttaa tggcggttac cgtgtttgag gagacttctg
      841 gctgggcgaa tagttccaga cccagggctt taatggcatt tctggtggcc agggcagcta
      901 actggtgtct tttcacccgg ctttccagtc cttcttccat cactgcgcat aaggcttcgc
      961 gcatggcgta catcaatgac acggatggtg tgtagggtgt ctctgggggt gtgcccgatc
     1021 cactcttacg gtaccttttc atatcaagat aataggtgct ggttggggtt ttatccacca
     1081 ctttccaggc gtcctcgctt aatgttatag cggccatgcc cggtggggcg gccagacatt
     1141 tctgggagcc ggtaacacaa atatcgatgt tataaccatc tacgaagaca tcatctcccc
     1201 ctagggagga aacagtatcc accacgtaga gaacatcaaa atcattaacg atc
//
LOCUS       DAOQVW010000526         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_157341_length_1252_cov_3.233918, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000526 DAOQVW010000000
VERSION     DAOQVW010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157341_length_1252_cov_3.233918"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKK85_09260"
     CDS             complement(<1..233)
                     /locus_tag="PKK85_09260"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HNS26240.1"
                     /translation="MDADYIAKCGQIASVLEVSGHPKPGNVHRTRDFPDMAFEDFLIS
                     GVVIGDNLRRAAHRGSRYHDPGKWHKIGLGKLI"
     gene            complement(243..1226)
                     /gene="hemB"
                     /locus_tag="PKK85_09265"
     CDS             complement(243..1226)
                     /gene="hemB"
                     /locus_tag="PKK85_09265"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNS26241.1"
                     /translation="MIFPFRRMRRLRKTPQIRKILQETTLNPEDFIYPLFIKEELEPG
                     DNEHINTMPGQYRYSLEDAVDEACRLEELGLHSVILFGMPREKDEVGSMAFHPDGIVQ
                     RGVARLKEETDLVVITDVCLCQYTSHGHCGVVEGGQILNDETLHYLSETALSHARAGA
                     DMVAPSDMMDGRVGAIREALDNSGFEDTIIMSYAAKYASSFYAPFRDAVCSEPSFGDR
                     RTYQMSPHTSNEALREVELDIEEGADILMVKPALAYLDIIKMVKDEYRMPTAAYQVSG
                     EYSMLISGIEAGYLTMDSIYESLLSIKRAGADLIISHFTPMFLEGQLDSCF"
BASE COUNT          295 a          333 c          293 g          331 t
ORIGIN      
        1 tttatcagtt ttcctaagcc tattttatgc catttacccg ggtcatggta gcgtgagcca
       61 cgatgggcag ccctccgcag gttatcccca ataaccactc cacttattag gaaatcctca
      121 aaggccatgt caggaaaatc cctggttcgg tgcacgttac ccggcttggg atggccgctt
      181 acctccaaca ccgaggctat ttggccacac ttagctatat aatcagcatc cacaattatc
      241 cctcaaaaac agctatccag ctggccttcc aggaacatgg gggtgaagtg gcttattatc
      301 agatccgccc cggccctttt aatggacagt aaagactcgt aaatagagtc catagtaagg
      361 taaccggctt cgataccaga tattaacatg gaatactcgc cactgacttg gtaagcggcg
      421 gtgggcattc gatattcatc tttcaccatc tttatgatgt ccaggtaggc tagggctggt
      481 ttcaccatca aaatatcagc accttcctct atgtccaatt caacttccct taaagcctca
      541 ttggaggtgt gaggactcat ttgataggtt cttcggtccc cgaaggaggg ttctgaacac
      601 accgcatccc ggaagggtgc gtagaagctg gatgcatact tggcagcgta ggacatgatg
      661 atggtatcct caaatccgct gttatccagg gcttcccgga tagcccccac tcggccatcc
      721 atcatatctg aaggcgccac catatctgcc ccggcccggg catgggatag ggctgtttcg
      781 gataggtagt gcagggtctc atcgtttagg atctgacccc cctccaccac tccacagtgt
      841 ccgtggctgg tgtactggca cagacacaca tcagttataa ccaccaggtc agtttcttcc
      901 ttcaacctgg ccacgccacg ttggactata ccatcaggat ggaaggccat ggagcccact
      961 tcatccttct cccgtggcat gccgaatagt atcacagaat gaagtcccag ttcttccagt
     1021 cgacaggctt catccacagc atcctccagt gagtagcggt actggccggg catggtgtta
     1081 atatgttcat tatcaccggg ttccagttct tccttaatga acaggggata gatgaagtct
     1141 tctggattta aggttgtttc ttggaggatt ttcctgatct ggggggtttt tcttaacctg
     1201 cgcatgcgac gaaaagggaa tatcatgttc ttaccaccta gtgttaattg ca
//
LOCUS       DAOQVW010000527         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_157485_length_1252_cov_1.594820, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000527 DAOQVW010000000
VERSION     DAOQVW010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157485_length_1252_cov_1.594820"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PKK85_09270"
     CDS             complement(<1..886)
                     /locus_tag="PKK85_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNS26242.1"
                     /translation="MTMESETKDKVALVGTPCHMTAAAKIDTFSDYLGESPLDIKIGL
                     FCMENFSYSYMKQLLEENGYDMKDVIECRVEKGYFWFYLTEGRLFKIPLKEAKSCMRK
                     NCQICMDYTSEDSDISVGSVGSPEKWSTIIVRTKKGLKLLEDAEEDNYITTKPIEDSG
                     LKILENLARKKKKENKAEITKREHVGRPVLYRRYISEDDFAEEVRECQFKDLKGDVVD
                     VGACVLCGACLLACPENIIVIEDRKPQLKGKCPEGCNLCYVACPRTYNPEKEPLIESD
                     KKPLGNYLKIVSAQAPMIK"
     gene            complement(895..>1252)
                     /locus_tag="PKK85_09275"
     CDS             complement(895..>1252)
                     /locus_tag="PKK85_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tributyrin esterase"
                     /protein_id="HNS26243.1"
                     /translation="RDAGSRTGEIMKNGTIIIGGNSGFMTGLFMMGGRIIILGDISDD
                     AGESIIRGVIYIKGAIKSLGKNAKVKEINDADKKELEELLPQYGFNLEREEYADFKKI
                     VPLSKRPFYGKESEEG"
BASE COUNT          261 a          292 c          249 g          450 t
ORIGIN      
        1 cttttatcat gggtgcctgg gcagatacta ttttcaggta gttacccagt ggttttttat
       61 ctgattcaat gagtggttct ttttctggat tataggtccg gggacaggcc acgtaacaga
      121 ggttgcatcc ttctgggcat tttcccttaa gctgtggttt cctgtcttct atcactatga
      181 tgttctctgg acaggccagt aaacaggctc cgcagagtac gcaggcacct acatctacca
      241 catctccttt aagatcttta aactggcatt cacgtacttc ttcagcaaaa tcatcctctg
      301 agatgtatct gcggtagagt actggtcttc ccacatgctc ccttttggtg atctctgctt
      361 tattttcttt tttcttcttg cgagccaggt tttcgagtat cttaagtcct gaatcttcta
      421 ttggcttggt tgttatgtaa ttatcttctt ctgcatcttc taataatttt aatccctttt
      481 ttgtccggac tatgatggtg gaccattttt caggagatcc tacagaacct actgatatgt
      541 ctgagtcttc agaggtgtag tccatgcata tctggcagtt ctttctcatg cagcttttag
      601 cttcctttaa tgggatcttg aacaaacggc cctcggtaag gtagaaccag aaataacctt
      661 tctctacccg gcattcgatg acgtccttca tgtcatagcc attttcctct aggagttgtt
      721 tcatgtagga gtaggaaaaa ttttccatgc agaaaaggcc tatctttata tccaggggtg
      781 attcacccag ataatctgag aatgtgtcta ttttcgctgc ggctgtcata tggcatggtg
      841 tccctaccag ggccacttta tcttttgttt cactttccat tgtcatcaat tctcctatcc
      901 ttcttctgat tctttaccat agaatggtct tttacttagg gggactatct tcttgaaatc
      961 agcatattct tcacgttcta gattgaaccc gtattgggga aggagctcct ctaattcctt
     1021 tttatcggca tcattaattt cttttacctt ggcattcttt cccaggcttt taatggctcc
     1081 tttgatgtag atgactcccc tgattatgga ttcgccggca tcgtctgata tatctcccag
     1141 gatgatgatg cgtccgccca tcatgaagag tccggtcatg aatcctgagt ttccaccgat
     1201 gatgatggtt ccatttttca ttatctcccc ggtcctggag ccggcgtctc tt
//
LOCUS       DAOQVW010000528         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_157723_length_1250_cov_3.709623, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000528 DAOQVW010000000
VERSION     DAOQVW010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157723_length_1250_cov_3.709623"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1208
                     /locus_tag="PKK85_09280"
     CDS             51..1208
                     /locus_tag="PKK85_09280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019268756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HNS26244.1"
                     /translation="MAEDITTLVTGLGFPSMEAFLAVMFLILAIFGLIVVVSTIKPVL
                     SMFPYTYPNARVRARMGRIFSEKQFSEIIESGNLEEVKNYLRGYPDYAKYIDQYPLEK
                     ALDIQLAENYDLVARISPENSRDSFKFLLKKWDIRNLKSIIIAKEAGLSAEETMDLVV
                     PFGELTDKLDALIDADNINEVINTLEGTEYPQIMEDAIPIYQETGMLLPLESSLDKYL
                     LENLLRTVATPEDDNTSYLKNYVGDLVDSTNLKIILRAKVDGLKYEDIEPYMISDGYQ
                     IREWKLKDLMEAEDVSGVVSGLEGTDYSTLLSDAMADYNDSGSISAFETALDNHVDDT
                     AQKISLKNQFGIGPMIGFLSRKEREIKNLKIIARGKREEGYSPAQIKEMLI"
BASE COUNT          406 a          245 c          284 g          315 t
ORIGIN      
        1 tgagatctga agttgctggt atactgttcc aatcgtaggg gagatcacac atggcagaag
       61 acattactac actagtaact ggtctgggat tcccctctat ggaagctttt ctagcagtca
      121 tgtttctgat actggctata tttggtttga tagtggttgt atctaccatc aaacccgttc
      181 tgagtatgtt cccctacacc tatcccaacg ccagggttag agccagaatg gggcggatat
      241 tcagcgaaaa acagttttca gagatcattg aatccgggaa cctggaagag gtaaaaaact
      301 acctacgcgg atatcctgat tacgcaaaat atatcgacca gtacccctta gaaaaggctt
      361 tagacatcca acttgccgaa aattatgact tagtagccag gatatctcca gaaaacagca
      421 gagactcttt taagttttta ctaaaaaaat gggatatccg gaatttaaaa agcataataa
      481 tcgccaaaga agcaggttta agtgcagaag aaaccatgga cttggtggtt ccatttggtg
      541 aacttaccga caagctggat gccttaattg atgcggataa catcaacgaa gtgataaaca
      601 cactggaagg aacagaatat ccccagatta tggaagacgc cattccaatc taccaggaaa
      661 ccgggatgct gttaccatta gaatcctcac tggataagta tctcctggag aaccttctaa
      721 ggacggtggc tacaccagaa gatgataaca cttcctactt aaaaaattac gtcggagatt
      781 tggtggacag tactaaccta aaaatcatcc taagggccaa ggttgatgga ttgaagtatg
      841 aggatattga accctacatg atcagtgatg gttaccagat tagggagtgg aaacttaagg
      901 accttatgga agcagaagat gtatctggag tggttagtgg cctggaagga actgattatt
      961 ccaccctact atcagatgct atggctgatt acaacgatag tggttccata tccgcctttg
     1021 aaaccgccct tgacaaccac gtggatgata ctgcccaaaa aatatctttg aagaatcagt
     1081 ttggaatcgg gcccatgatc ggttttctaa gtagaaaaga aagggaaata aaaaacctga
     1141 agatcatcgc tcgtggaaaa agggaagaag gatattctcc tgctcagatt aaggagatgt
     1201 taatatgaaa tcagcaatag cagtgatggc tgatccggac acagttaccg
//
LOCUS       DAOQVW010000529         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_157749_length_1250_cov_3.112971, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000529 DAOQVW010000000
VERSION     DAOQVW010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157749_length_1250_cov_3.112971"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKK85_09285"
     CDS             <1..255
                     /locus_tag="PKK85_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNS26245.1"
                     /translation="AFILGLIVNYILSISWVFNNRKLNSSTLEFGVFSLIGMVGLGLN
                     EVFIWFFTAEVGFYYLISKIISAIIVLFWNFFARKYVLFK"
     gene            279..>1250
                     /locus_tag="PKK85_09290"
     CDS             279..>1250
                     /locus_tag="PKK85_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNS26246.1"
                     /translation="MSENRKAVIIGAGPAGLTAAYELLDKTDIQPVIFEATDAIGGIS
                     KTVNHNGNRIDIGGHRFFSKSDRVMEWWQNILPLQGSPARDDLILGREVPTVEVSKKK
                     EVASMEIITGAAPDPEKDDQVMLNRSRLSRIFYTRKFFDYPVALNLKTISNLGLINTL
                     KIGLSYLKSTLKPIHPEESLEDFFINRFGRELYLTFFKDYTEKVWGVPCEQINADWGA
                     QRIKGLSIKKALTHAIMVNRQNNEDLAQKEVETSLIGQFMYPKYGPGQMWEEVAQVLT
                     RSGAIINHHHRVVGIEAEKDLVHAVLVKNEETGEQIRVEADYFFSTMP"
BASE COUNT          363 a          253 c          291 g          343 t
ORIGIN      
        1 gcctttatat tgggtttaat tgttaattat atccttagta tctcctgggt ttttaataat
       61 cgcaagttga acagttccac cctggaattt ggggttttta gtctaatcgg gatggtaggt
      121 ttgggattaa atgaggtttt catctggttt ttcacagccg aagtaggttt ctactaccta
      181 atttccaaga tcatatccgc aattatcgtc ctgttctgga acttcttcgc caggaagtac
      241 gtcttgttca aatgaaaaat tcgaatataa gtgatgatat gtctgagaat cggaaagctg
      301 tcataatagg tgcaggtcct gcaggcttaa ctgcagccta cgaactcctc gataaaacag
      361 atattcaacc agttatattc gaggccactg atgccattgg gggcatctct aaaactgtga
      421 accataatgg taacaggatc gatattggag gtcacagatt tttctccaag tcggatcggg
      481 tgatggagtg gtggcagaat attcttcccc tccagggcag tccagcccgg gatgatctga
      541 tactgggaag ggaagtgcca actgttgagg taagtaaaaa aaaggaagtt gcctccatgg
      601 aaattatcac tggcgccgca ccggaccctg aaaaggatga tcaggtaatg ttgaatcgca
      661 gcaggttatc tagaattttt tacacccgta aattttttga ctacccggtg gccctgaacc
      721 ttaaaaccat ctcgaacctg ggccttataa acacccttaa aataggttta agctacttga
      781 aatccacact aaagcctatc catccagaag aatccttgga ggacttcttc attaaccgct
      841 ttggacgtga actttacctc accttcttta aggactacac cgaaaaggtg tggggtgtcc
      901 cctgtgaaca gatcaacgcg gactggggcg cccaaaggat caagggatta tcaattaaaa
      961 aggctttaac acatgcaata atggttaaca ggcagaataa tgaagacctg gcccagaaag
     1021 aagtggaaac tagtttgatc ggacagttca tgtaccctaa gtatggtcct ggccaaatgt
     1081 gggaggaagt agcccaggtg ctcactaggt cgggtgctat aataaaccac catcatcgtg
     1141 tggttggaat agaagccgag aaagaccttg tgcatgctgt tttggttaaa aatgaagaaa
     1201 ctggtgaaca aataagggtt gaagccgact attttttctc aaccatgcct
//
LOCUS       DAOQVW010000530         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_158394_length_1247_cov_3.250839, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000530 DAOQVW010000000
VERSION     DAOQVW010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158394_length_1247_cov_3.250839"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKK85_09295"
     CDS             <1..592
                     /locus_tag="PKK85_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNS26247.1"
                     /translation="KVARQSYRGPLNREAFPPCVKTALEGIKSGGRNDAIVLFITPFV
                     SYARLCPDIFKQNVTVRVSDKDPQLQVVHQEVLPLIYEAAERCVPPLFEDQPQEKINI
                     NAKIGFGMHSTLKLENEGETKWYTPMSCEKIKLHLPYLCRPDDDCKKIGNPLIYYNRK
                     NWILSREQAESGDKKEDEGSSGEEVMENQDNDWIDV"
     gene            620..>1247
                     /gene="priS"
                     /locus_tag="PKK85_09300"
     CDS             620..>1247
                     /gene="priS"
                     /locus_tag="PKK85_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HNS26248.1"
                     /translation="MEFQRATPEERRLYYREEWDVKQIPRFIRDSLYQREFGFDHHGR
                     GPNDRYRTFRNEDHLRKFMRYRTPFAAYTSVAFYQKPNRREGWIKSELVFDVDAKDLP
                     IRSCACDSVCELCLDQAKNIVSDLKDTLTSDLGLKEIYMIYSGRGYHLRVQDEEVLGT
                     DSEVRSQIVKYLVGAEVPRSEYGQEGITYNLEHFAIPFGYHQVFTQRIK"
BASE COUNT          364 a          313 c          305 g          265 t
ORIGIN      
        1 gaaagtagcc agacaatcct accgtggacc tctcaaccgg gaggcattcc ccccctgtgt
       61 taaaactgcc ctggagggca ttaaatcagg gggaagaaac gatgccattg tactgttcat
      121 cacccccttt gtatcctacg cccgtctgtg tccggatatt ttcaaacaga acgttacagt
      181 ccgtgtctca gacaaagacc ctcaactgca ggtggtgcac caggaggtgc ttcccctcat
      241 ctacgaagcc gcagaaaggt gcgtcccacc cctatttgag gatcaacccc aggagaagat
      301 caacatcaac gccaagatag gatttggcat gcactccacc ctgaaacttg aaaatgaggg
      361 ggagactaaa tggtacaccc ccatgagttg tgagaagatc aagctgcacc taccctacct
      421 atgccgacca gatgatgact gtaaaaagat tggaaacccc ctgatctact acaaccgtaa
      481 aaactggata ctgtccaggg aacaagcaga atctggcgat aaaaaagaag atgaaggttc
      541 tagtggagaa gaagtgatgg aaaatcagga taacgattgg atagatgtgt agctaaaaaa
      601 ttataataca ggaactaaca tggaatttca aagagccacc cccgaggaga ggcgacttta
      661 ctatagggaa gaatgggatg tcaagcagat cccacgtttc atacgtgact cactctacca
      721 acgggagttc ggattcgatc atcatggaag ggggcctaac gacaggtaca ggaccttccg
      781 taacgaggac catctgcgca agttcatgcg ctatcgcacc cccttcgcag cctacacatc
      841 agtggccttc taccagaaac ccaaccgcag ggagggatgg attaaatcgg agctggtttt
      901 tgatgtggat gctaaggacc tgcccattcg ttcctgtgcc tgcgacagtg tctgtgaact
      961 ttgcctggac caagccaaaa acatagtttc cgaccttaaa gatactttaa ctagtgattt
     1021 aggccttaaa gagatttaca tgatatattc tggccgggga taccacctcc gggtacagga
     1081 tgaagaggtc ttgggcacag acagtgaggt gcgctcccag atcgtgaagt acctggtggg
     1141 agccgaggta ccccgcagtg agtacggcca ggagggaata acctataacc tggagcactt
     1201 cgccataccc ttcggatacc accaagtctt cacccagcgt attaaac
//
LOCUS       DAOQVW010000531         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_158537_length_1247_cov_1.845638, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000531 DAOQVW010000000
VERSION     DAOQVW010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158537_length_1247_cov_1.845638"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKK85_09305"
     CDS             complement(<1..196)
                     /locus_tag="PKK85_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26249.1"
                     /translation="MDNRGEKDRGIFLAFTSTRYGYSIRPTVSYIRYEYLNISDCYVG
                     EKYLKDKLIFYAAPNPTSLTR"
     gene            300..602
                     /locus_tag="PKK85_09310"
     CDS             300..602
                     /locus_tag="PKK85_09310"
                     /inference="COORDINATES: protein motif:HMM:NF028174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HNS26250.1"
                     /translation="MIDDVLDGYRNYQTLSVALELDVFEEIAENGPSSARDIADAALV
                     NRMFIRIILTALEDMGLLKSNQDKYFLTEPTETFLLQRNPLYQGDLILSMGSYKSG"
     gene            612..>1247
                     /locus_tag="PKK85_09315"
     CDS             612..>1247
                     /locus_tag="PKK85_09315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNS26251.1"
                     /translation="MKILNLKGTAIKLHQELDEFQIRSMTQQCIRGEVQTVVKELVSY
                     PGFPKSEYLLDIGGSQGIYSIALCQENNKLLARILEQDHVASLTSSFIWEYGMENRID
                     VRIGDIKDLKKSDTWKYDIVLISNLLYNYPTEKGDVLENISQILNPGGILVFNHRFYS
                     LNCDVKPGSGVREIDRALNGFGLHLCHPEGFKEIFEELGFLNVYSKPYETAS"
BASE COUNT          395 a          224 c          266 g          362 t
ORIGIN      
        1 atcttgttag tgatgtgggg tttggagcgg cataaaaaat aagtttatcc tttaaatatt
       61 tttctccaac ataacaatcc gatatgttta aatattcata acgtatatat gagactgtcg
      121 gtcgtatgga atatccatac ctagtagagg tgaaagctag gaatatcccc ctatcttttt
      181 cacccctatt atccattact ttctttttgg cgtgaaaatt gaaaagagac aaaatgtacg
      241 atgtaaaatt tgattacacg aataaaatcg ctgaaattga gattcctgag ggaatctccg
      301 tgatcgacga tgtactggac gggtatagaa attatcagac cctgtctgta gccttggaac
      361 ttgatgtctt cgaagaaatc gcagaaaacg gaccatcttc tgcacgtgat attgcagatg
      421 cggccttagt taataggatg ttcatcagaa ttatccttac agcattagaa gacatgggcc
      481 ttttgaagtc gaatcaggac aagtactttc taactgaacc tacagaaacg tttctcctgc
      541 agagaaaccc actctatcag ggtgatctga tcctctctat ggggagctat aaatcaggtt
      601 agaataatct gatgaaaata ctgaacctga aaggaacggc tatcaagctc caccaggaat
      661 tggatgaatt ccagataaga tccatgaccc aacaatgtat cagaggagaa gtacaaaccg
      721 ttgtgaagga attagtatct taccctggat ttccaaagtc cgaatatcta ttggatatcg
      781 gcggaagcca gggtatatac tctattgcac tttgtcagga gaacaacaag ctattagcca
      841 ggatactgga acaagaccat gttgcgtcat tgacctcgtc ctttatatgg gaatacggga
      901 tggaaaaccg gatcgatgtg cggataggtg atattaaaga cttaaagaaa agtgacacat
      961 ggaaatatga tatcgtgctg atttccaatc ttctatataa ctatcctacg gaaaagggtg
     1021 atgttctgga aaatatatcc cagatattaa atccaggcgg aattttagtg ttcaaccatc
     1081 gtttttacag tttaaactgt gatgttaaac ccggaagtgg ggttcgtgaa attgaccggg
     1141 ctttaaacgg ttttggacta cacttatgtc accctgaagg ttttaaagaa atttttgaag
     1201 agttgggatt cttgaacgtt tattcaaaac cttatgagac ggcatcg
//
LOCUS       DAOQVW010000532         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_158550_length_1247_cov_1.652685, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000532 DAOQVW010000000
VERSION     DAOQVW010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158550_length_1247_cov_1.652685"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKK85_09320"
     CDS             complement(<1..409)
                     /locus_tag="PKK85_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNS26252.1"
                     /translation="MVTAVIMAGGKGTRMGFKGEKPLISVRGKPMIRHVCEAVRSSRE
                     VDDLVVATSRNTPLTTDFARKSGLKVIETPGEGYVNDLGFLIGELSSNNPPTNNQDDI
                     LVTITADLPLITGEILDEVITEYKNCGKPAMCVA"
     gene            640..1038
                     /locus_tag="PKK85_09325"
     CDS             640..1038
                     /locus_tag="PKK85_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNS26253.1"
                     /translation="MVLTQEMMDTIEKNNIVWLATASTDDVPNVVPIGLARPLDSGTV
                     LLVANFMNKTFKNLKSNPKASIAVGNVSECPYQFKGDVEIYESGKYFDDAVQWAKSVM
                     AQLNPKAAVLLKVNEIYSVQPGPDAGKKIE"
BASE COUNT          393 a          238 c          252 g          364 t
ORIGIN      
        1 cagcaacaca catggctggt ttaccacagt ttttatattc tgtaattacc tcatctaaaa
       61 tttccccggt gatgaggggt agatctgcgg tgatggtcac caggatatcg tcctggttat
      121 tggttggtgg gttgttactt gatagttccc ctattaaaaa tccgagatcg tttacatacc
      181 cttcccctgg ggtttctata accttcaggc cggattttcg ggcaaaatca gtggtgagtg
      241 gtgtgtttct gctggtggcc accactaaat catccacttc ccgggacgac cgtacggctt
      301 cgcaaacgtg cctgatcatg ggcttgcccc ggacggaaat caggggtttt tcacccttaa
      361 atcccatcct ggttccttta ccaccggcca tgattactgc agttaccata ttattttttc
      421 cctattattt aagaaaaaat tttattaaaa atccttcttg aaatttaaaa aatcgttata
      481 tagttgtttg tagttataac cccatattaa aggtttttat taaatatccc ctcagtcatt
      541 tcagtaacca ggatacagtt tagtatattt tactgaccaa aatgtacatt atattattaa
      601 atcaatatgg gtatgtagaa aaattaaggg tgtgatagaa tggttttaac tcaagagatg
      661 atggacacaa tagaaaaaaa taacatagtt tggctggcaa ctgccagtac cgatgatgta
      721 cctaacgtgg ttcccatagg actggccaga cccctagata gtggaacggt tttgctggtg
      781 gctaatttca tgaacaagac ttttaaaaat cttaaaagca accccaaagc gtcgatagcc
      841 gttggtaacg tatcagaatg cccatatcag tttaaaggag atgtggagat atatgaatct
      901 ggtaaatatt tcgatgatgc cgtccagtgg gctaaaagcg taatggcaca attaaatccc
      961 aaagccgcag tcctgctcaa ggtaaatgag atatattctg tacaaccagg cccagatgcc
     1021 ggtaaaaaaa tagaatgaaa agcatttaac ttaagttaag tctggcttta agttttgacc
     1081 tccagagaag ggaacgggta catactcgtt ctcttttttt aaccaataaa atatagcgat
     1141 agttaacagt taaagatata acatgttcag gagatgcaag tccgaagata ataaaaagat
     1201 aataagccaa cgagttacaa gccaaaaaaa cagcaaatca gaaggtg
//
LOCUS       DAOQVW010000533         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_158702_length_1246_cov_2.361041, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000533 DAOQVW010000000
VERSION     DAOQVW010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158702_length_1246_cov_2.361041"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKK85_09330"
     CDS             <1..232
                     /locus_tag="PKK85_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HNS26254.1"
                     /translation="EALVLAGSLMGGKIARAIDEIKKEHNVLVISLNMPGSVTEKADL
                     VVTDPVQAGVMAVMAVADTAIFDIKKIKNKNF"
     gene            complement(277..558)
                     /locus_tag="PKK85_09335"
     CDS             complement(277..558)
                     /locus_tag="PKK85_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26255.1"
                     /translation="MDKKAEELLEKCENIEDSSVMGSCKAMLEMMAKSNETIEDKPNE
                     TYLQMAETLTPQDVPKVLELALKIRESGDITDPDLKIAASKLIRAIEMS"
     gene            728..1231
                     /locus_tag="PKK85_09340"
     CDS             728..1231
                     /locus_tag="PKK85_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HNS26256.1"
                     /translation="MAPYIDSMDGPVVKAAENALDMENIKYILPYVSPADEGELKDAF
                     EKTLSVRELSGEAAELADYWFFETAVRLHHKGEEESYTGLKAAGTDWGPIIPKIDQAI
                     ETENIDEFLDFLLNFIKEDIQGRFDDVCSKKDYDEDDVEDAREYVNAREEFISYTRKF
                     YEYVEKG"
BASE COUNT          408 a          222 c          259 g          357 t
ORIGIN      
        1 ggaagcactg gtactggccg gttccctcat gggtgggaaa atagcaaggg ccatagatga
       61 aataaaaaag gaacataacg tcctggtcat aagcctgaac atgcccggta gtgtaactga
      121 aaaggcggat ctggttgtca cagacccggt acaggctggt gtgatggcag ttatggccgt
      181 ggcagataca gccatattcg atataaagaa gattaagaat aaaaattttt agaggttatc
      241 attaaataaa aaagggttaa aataataaaa aaaagtttaa ctcatttcta tagcccttat
      301 cagtttgctc gcggcgattt ttaagtcagg atcagttata tctccacttt cccttatttt
      361 aagtgccagt tccagaacct ttggtacatc ctggggggtt aaggtttccg ccatttgcag
      421 gtaagtttcg tttggtttat cttcgatagt ttcgttactt ttagccatca tttctaacat
      481 ggccttgcag gaacccatta cactactatc ctctatgttt tcacattttt ctaacaattc
      541 ttcggctttt ttatccatga ttatcaccac taattataat ttaatgattt gaaaatttat
      601 agttttggga aagccatttc ctcctaattt tctagccaaa aattctatat aaaacaaaaa
      661 caatataatg agtgcatagc aactctctaa aaatttaata aatctataga ttaatcgggg
      721 gattaatatg gcaccatata tagatagtat ggacggacca gtggtaaaag ctgcagaaaa
      781 cgccttggat atggagaaca ttaaatacat tctcccctac gtttctccag ccgatgaagg
      841 agaacttaaa gatgcctttg agaaaactct ttcggtaagg gaactcagtg gtgaagcagc
      901 tgaattggcc gattactggt tttttgaaac ggcagtccgt ctacatcata aaggagaaga
      961 ggaatcatac actggactta aagcggcagg tactgattgg ggccctatca tccccaagat
     1021 cgaccaggcc atcgaaaccg aaaacataga tgaattcctt gatttcctct taaatttcat
     1081 aaaggaagat atccagggcc gatttgacga tgtgtgttcc aaaaaggatt acgatgaaga
     1141 cgatgtggaa gatgccaggg aatacgtaaa tgcaagggaa gaattcataa gctatacacg
     1201 taaattttat gaatatgtag aaaaaggatg aaaaagaatc aggtga
//
LOCUS       DAOQVW010000534         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_159614_length_1242_cov_1.784330, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000534 DAOQVW010000000
VERSION     DAOQVW010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159614_length_1242_cov_1.784330"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..>1242)
                     /locus_tag="PKK85_09345"
     CDS             complement(31..>1242)
                     /locus_tag="PKK85_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNS26257.1"
                     /translation="EDNMVGFIRWCATTKLGTLIIMRFNEDAVVKQFKKETGITAYSG
                     IAAELKFNAWLVKKIEKDPDSLVEILYVFENITPEIHNNLTGGVDSKNVVCDALGLDM
                     EYILGLGLTNVANYRVATDYETGNLTQKQIKQIGINAANQALALFAADGIDLEKDDYD
                     LTVFTTAGYVRVNGQVMDMTMDGIYQILGSRLSRATLLPVHNARYNPLYFQFSLERNG
                     TVISKTLYYDPETGKFTAKNESACIIDQVILYDPPYDALMAWLWHNHVCGGSSPGYLV
                     TNYVYDNFPIGEDESYAFIGNSISCRDDIYSYLLGVSAGAGTYFSQRMTKDTTGDQIG
                     ILSIYDSKTNTRRVVIINWTGPQFAKGSNSYEEYIRLYKGDFSSPNLISAPTITLAAD
                     TWVTEEEWNRI"
BASE COUNT          297 a          258 c          277 g          410 t
ORIGIN      
        1 tcttcattat cggtgtgtga accaccactt tcagattctg ttccactctt cttcagttac
       61 ccatgtatct gcagctaagg ttatcgtcgg tgcagagatc aggttgggtg aggagaagtc
      121 tcctttgtaa agtctaatgt attcctcata agagttagaa cccttagcaa attgcggacc
      181 agtccagtta ataataacca ctcttctggt attggtttta ctatcataaa tactgaggat
      241 tccaatttga tcaccagtcg tgtctttggt cattcgttgg ctgaagtagg ttcctgcacc
      301 tgcagaaaca cctaacaggt atgagtagat gtcatctctg cagcttatgg aattacctat
      361 aaatgcgtaa ctttcgtctt ctccaatagg gaagttatca tagacgtagt tagtgactag
      421 gtacccgggt gaactaccac cacagacgtg gttgtgccat aaccatgcca ttaaggcatc
      481 gtatggtggg tcataaagga ttacctggtc tatgatgcat gctgattcat ttttggctgt
      541 aaattttcct gtttcagggt cgtaataaag ggttttgctg atcacggtac catttctttc
      601 caagctgaac tggaagtaca gtgggttgta tcttgcattg tgtacgggta gtagagttgc
      661 tctggaaagt ctggatccta ggatctggta gattccgtcc atggtcatgt ccattacctg
      721 tccgttgaca cgcacgtaac cggcagtggt gaagactgtg agatcatagt cgtctttttc
      781 cagatcgata ccatctgcgg caaatagggc gagggcctgg ttagcagcat taattccgat
      841 ctgtttgatc tgtttctgag tcaggtttcc agtttcgtag tcggttgcaa ctctatagtt
      901 tgctacgtta gtcaggccca gacctaatat gtattccatg tccagtccta gagcgtcaca
      961 tacaacgttt ttggagtcta ctcctccagt taggttgtta tgaatttcag gggttatatt
     1021 ttcgaaaaca tatagtatct ccactaggga gtcggggtct ttttctatct ttttgactaa
     1081 ccatgcattg aactttagtt cagcggcaat accagaatat gcggttatac cagtttcttt
     1141 tttgaattgt ttaaccacag catcctcgtt gaacctcatt attatgaggg ttcctaattt
     1201 ggttgttgca caccatcgga tgaatccgac catgttatct tc
//
LOCUS       DAOQVW010000535         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_159689_length_1241_cov_2.922428, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000535 DAOQVW010000000
VERSION     DAOQVW010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159689_length_1241_cov_2.922428"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKK85_09350"
     CDS             complement(<1..315)
                     /locus_tag="PKK85_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNS26258.1"
                     /translation="MSQFRLRDLLIDRELFESIRTAALQEEGWDVEKQDEVKRFERNV
                     LQRMRKKRPVKKYKKLGVKKSDLVEIIQLADISSLELFQGPSNLELAKRHSEWCNHCG
                     KCC"
     gene            complement(327..>1241)
                     /locus_tag="PKK85_09355"
     CDS             complement(327..>1241)
                     /locus_tag="PKK85_09355"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNS26259.1"
                     /translation="LALKSLDIGKGDEVLVPTLTFAATANAVIYCNAEPVFVDSHPEY
                     WCMDPYSLEEKVTSRTKAIIPVHLYGHPCDMDPIMDVAEDHDLYVVEDAAEAHGALYK
                     GKKVGTFGDISCYSFYGNKIITTGEGGICITNDDSLEDKMRILRDHGMNTSKRYWHDV
                     IGFNYRMTNLQAAIGVAQLQKLDQFIAKKRKIASYYQQMLKDLVDDGLIILHPEMSWA
                     NCVYWIYCILIEDKFNLKRDNVITELLSRKIDTRPFFYPITDMDPYQKVPLPHAEELS
                     RKGLSLPSSINLDYEDISKITDALKSIR"
BASE COUNT          307 a          300 c          249 g          385 t
ORIGIN      
        1 gcaacacttt ccacagtgat tacaccactc ggaatgtctc tttgcaagtt ctaaattgga
       61 tggtccctgg aaaagttcca ggctgctgat atctgccagc tggattattt ctaccagatc
      121 cgattttttt acacccaatt ttttgtattt tttcaccggc cttttcttgc gcatccgctg
      181 cagaacattt ctttcgaacc gcttcacctc atcttgtttc tccacatccc atccttcctc
      241 ctgtaaagcg gctgttcgga tactttcaaa taattcccgg tcaattaaca ggtcccttaa
      301 tcggaattgg ctcacccctc aatgaatcat ctaatacttt tcagtgcatc tgtaatcttg
      361 gaaatgtctt cataatcaag gtttatactt gaaggtaggc tgagtccttt tctagacaat
      421 tcctcagcat ggggcaatgg taccttctgg taaggatcca tatccgttat aggataaaag
      481 aaaggcctag tatcaatttt cctactcaac aattcagtta tcacattatc ccttttcaga
      541 ttaaacttat cttcgattag aatacagtaa atccagtata cacaatttgc ccaggacatc
      601 tcggggtgta ggatgattaa gccatcatca acgaggtctt taagcatttg ctggtagtaa
      661 ctggcgattt tccttttttt tgcaatgaac tgatccagct tctgcaactg ggccaccccg
      721 atggcagctt gtaggttagt cattcggtaa ttaaacccga tgacatcatg ccagtatcgc
      781 ttgctggtgt tcataccatg atcccgcagg attctcatct tatcttccag ggaatcatcg
      841 ttggttatac agatcccacc ctccccggtg gttattattt tatttccata gaatgaatag
      901 caggatatgt ccccgaaggt acccaccttt ttgcccttgt atagggcgcc gtgtgcttca
      961 gcagcgtctt ctaccacgta aaggtcatgg tcctctgcca catccatgat ggggtccata
     1021 tcacagggat gtccgtagag gtggactgga ataatagctt tagtccggct agttaccttt
     1081 tcctccaggg agtaagggtc catgcaccag tattcaggat gtgaatctac aaaaacaggc
     1141 tcggcattac agtaaataac ggcgttggcc gtggcagcaa atgtgagggt gggaactagg
     1201 acttcatctc ctttacctat atccaggctt ttaagggcca g
//
LOCUS       DAOQVW010000536         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_159832_length_1241_cov_1.344857, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000536 DAOQVW010000000
VERSION     DAOQVW010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159832_length_1241_cov_1.344857"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKK85_09360"
     CDS             <1..411
                     /locus_tag="PKK85_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HNS26260.1"
                     /translation="IAVHSMVDKIYSVEINPTAYDYLCQNITLNDVSGKIKPLYGNCR
                     ELAPVGVADRVLMGYIGNTHHYLDVALSALKDEGGVIHYHESVPDKLKFIRPVNRIKE
                     AVEGFEVNILNKRIIKKYSPGVYHVVVDAWIRKG"
     gene            513..>1241
                     /locus_tag="PKK85_09365"
     CDS             513..>1241
                     /locus_tag="PKK85_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNS26261.1"
                     /translation="MENQRRVQVIMEAIQENGLLDSKDIHQPVSAGEEDLLRVHSKDH
                     VEHIRRFCEMGGGNLDFDTTVSSESYRIAKLSAGGAIKAAELVLDGCESAYSVGRPPG
                     HHATRNRAMGFCIFNNLAITLEYLRQLRKIERFFIFDFDVHYGNGTADMFYEDPDVMY
                     ISIHQEPRTLFPGKGNVEEIGSGEGEGRNLCIPMPPGSNSHDYRYILENILGPVAQSF
                     SPDFYLVDVGFDGHWDDPLSSIRLD"
BASE COUNT          347 a          245 c          331 g          318 t
ORIGIN      
        1 atagccgtcc attccatggt ggataagatc tactccgtcg agataaatcc aactgcttac
       61 gattatctat gccagaatat cacccttaac gatgtttcag gtaagataaa accattatat
      121 gggaattgcc gggaattagc gcctgttgga gtggccgatc gggttttaat gggttatatc
      181 ggcaacaccc accattacct ggatgtggca ctttcggcct taaaggatga ggggggagtg
      241 atacactatc acgaatctgt accggacaaa ttgaagttca tccggccggt taaccggata
      301 aaagaagctg tagagggctt tgaagtgaat attttaaata agaggatcat caagaagtat
      361 tcccctggcg tgtaccacgt ggtggtggat gcctggatcc gtaagggata gatggtaagg
      421 gatatgatac taaaataaaa taagaatggg gggtttgcct gacagcggta gtttattctg
      481 aagactacag atttcacgat accggtgtac atgtggagaa tcagagacgt gttcaggtca
      541 taatggaagc tatccaggaa aatggtttac tggactcgaa ggacatccac caaccagttt
      601 cagctggtga agaagacctg ctgagagtac actccaagga ccatgtggag cacattagaa
      661 ggttctgtga gatgggcggc ggtaacctgg attttgacac cacggtatca tcagagtctt
      721 acaggattgc taaattatca gctggaggag ctataaaagc ggctgaactg gttttagatg
      781 gatgtgaaag tgcttattct gtgggaaggc ctcctggaca tcacgccact cgtaaccggg
      841 ctatgggctt ttgtatcttc aacaacctgg ccatcaccct ggaatattta agacagttaa
      901 ggaagataga aaggtttttc atattcgact tcgatgttca ctacggaaat ggcacggcgg
      961 atatgttcta tgaagacccg gatgtgatgt atatctccat ccaccaggag ccacggacgt
     1021 tattcccggg gaaaggaaat gtagaggaga taggtagcgg tgaaggggaa ggcaggaatc
     1081 tatgtattcc catgccccct ggatcaaaca gccatgatta ccgctatatc ctggagaata
     1141 tactgggacc agttgcacag agtttctccc ctgatttcta tctggtggat gtgggattcg
     1201 atgggcactg ggatgatccc ctttccagta tacgtctgga c
//
LOCUS       DAOQVW010000537         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_160110_length_1239_cov_3.254223, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000537 DAOQVW010000000
VERSION     DAOQVW010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160110_length_1239_cov_3.254223"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..1237
                     /locus_tag="PKK85_09370"
     CDS             383..1237
                     /locus_tag="PKK85_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNS26262.1"
                     /translation="MEGDDNITNKKGSEILEETVKEVKNHLDVAEVRVERAVVGIFFT
                     GVRLNTGHAGICATPIKEIPEAVCCPSSARSMPNAGRLTRRSVPKYLDDALSPVPMKR
                     TMGVAVLSALSSYCWEQGLVEGFDMKVGVDAIDCMELDDKSHPVVIGAIAPVLRILKA
                     RRKPFSVVELDKRTLKRDELPFYAPPERTQEVVPDADLLVVTGTTLLNGTLEGILELA
                     RHDAQVLVMGPSASLLPSAFFKRGVDVLGGDIITQPNRMLDTLAEGGSGYHIYGDSAE
                     RVVVTPQG"
BASE COUNT          346 a          288 c          346 g          259 t
ORIGIN      
        1 aagccccaag atagtgatgg agtagtaaaa attaaagtaa agagaaacca acgaacctaa
       61 cagtagtaat tggccagccc ggcctaaaca catccagaaa gtgagagttt cacatccaac
      121 caacgccagg taccactata cccccatctg ggagggggga tgttgctggg tccatgaaac
      181 aaagccccat ggtctggtgg atgaagctga tgggggatgg gttgctgtgc taagaatgat
      241 aataataatt aggatggatc tgagaatgat tatacacaaa tagtataaga tcctagttct
      301 tgggtgatga aatttttttt ttactcaaaa gaataatgat agatggaacc aaaaaaaaag
      361 aatccatcac cacaccaccc ccatggaagg agatgataac attaccaata agaaaggctc
      421 tgaaattctg gaagaaaccg tgaaggaagt taaaaatcac ttagacgtag ctgaggtacg
      481 ggttgagagg gcggtggttg gcatattttt cactggggtg cgcctgaaca caggccacgc
      541 cggtatctgt gccacgccca tcaaggaaat ccccgaggca gtgtgctgtc ccagctccgc
      601 ccggtccatg cccaatgccg ggaggttaac ccggaggtcg gtgcctaaat acctggacga
      661 cgcactatcc ccggtaccca tgaagagaac catgggagta gcggttttat ccgcactctc
      721 cagttactgc tgggagcagg gcttagtgga gggcttcgat atgaaggtgg gggtggatgc
      781 cattgactgc atggagctgg atgacaagag ccaccccgtg gttatagggg ccattgcacc
      841 ggttttaagg atactgaaag caaggagaaa gcccttctcc gttgtagagc tggataaaag
      901 aaccctgaaa agagatgaac tcccattcta cgcccccccg gagagaaccc aggaggtggt
      961 gccagatgcg gatctactgg tggttactgg aaccaccctc ctcaacggta ccctggaggg
     1021 catcctagag ctggcccgcc acgatgccca ggtactggta atggggccct cggctagtct
     1081 tctgccctcc gcctttttca agaggggagt ggacgtactg ggaggggata taataaccca
     1141 gcccaacagg atgctggata ccctggctga gggaggttct ggttaccaca tctacgggga
     1201 ctctgcagag cgtgtggtgg tgacacccca aggttaaca
//
LOCUS       DAOQVW010000538         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_162146_length_1230_cov_3.443404, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000538 DAOQVW010000000
VERSION     DAOQVW010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162146_length_1230_cov_3.443404"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1092)
                     /locus_tag="PKK85_09375"
     CDS             complement(178..1092)
                     /locus_tag="PKK85_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNS26263.1"
                     /translation="MNEASGDIIVMGDADGTYPLGEVYQFIQPILNDGQEFVMGSRLK
                     GEIKKGAMPALHRYVGNPLLTWILNILFKTGISDAHCGMRAMTREAWNKLDLKTAGME
                     FASEMVIEASRMNLKITEVPITYYPRGGESKLSSFSDGWRHLRFMMLYRPGPFLLIPG
                     VVALLVGATLASFVWFEGQSRMHSLILGSLLLLIGYQLLLSWLYFGAFAESYGVTSSR
                     LKKKVINYHSLEKELLLGLALLVVGVIIGLNVLYNWSAGGFGALSQIQQAMMAMILSI
                     LGIQTIFSGMFLSLLLLNQDENVKLGKK"
BASE COUNT          325 a          316 c          268 g          321 t
ORIGIN      
        1 ccagccctgg gtgtaccagt gcacctcgtg tcccataatt accagccgcc tggccacttc
       61 atagatcctc atctccgccc ctccagttac ccatgggtag gccgcatcgt agatgaaggc
      121 tattttcatt ataaaaaact cctggtttaa taaataatac gttaaattgt tcttagccta
      181 cttcttgccc agtttaacat tttcatcttg gttcagtagg agtaagctga ggaacatgcc
      241 tgagaaaatg gtctgaatac ccagtataga aagtatcatg gccatcatgg cctgctggat
      301 ctgggatagg gcaccaaagc ccccggcact ccagttgtat agcacattaa ggccaattat
      361 cacaccaaca accaacaacg caagtcccag gagcagttct ttttccaggg aatggtagtt
      421 tatgaccttc ttttttagtc tggaagatgt gactccatag gattcagcga aggcaccgaa
      481 gtagagccag gaaagtagca attggtatcc aatgagaagc aacaagctac ccagtatcag
      541 ggaatgcatc ctggattggc cttcgaacca tacaaaggag gccagagtgg caccgaccaa
      601 cagggcaaca actcctggta taaggagaaa gggtccaggc cggtacagca tcatgaatct
      661 aaggtgtctc catccatctg aaaatgaact gagcttggat tccccccctc taggataata
      721 cgtaattggg acttcggtga tctttaaatt catccgggat gcctctataa ccatctccga
      781 ggcgaattcc atccctgcag ttttaagatc taacttgttc caggcctctc gggtcatggc
      841 ccgcatacca cagtgggcat cggagattcc agttttaaat aggatgttca atatccaggt
      901 gagaaggggg ttgccgacat agcgatgaag tgctggcatg gcgccttttt taatttcacc
      961 ctttaaccgg gatcccatta caaattcctg gccatcattt aaaatgggtt ggataaattg
     1021 gtaaacttct cccagggggt aggttccatc ggcatctccc atcacaatta tatccccgga
     1081 ggcctcattc aaacctcgtt tataggcatt gccgtatcca cgatgacttt catgaacaac
     1141 ccttgccccg gctgcctggc ctcctgggca gtattatctg tagatgcatt atctaccaca
     1201 attatctcgg tttccaagcc catctcttcc
//
LOCUS       DAOQVW010000539         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_162169_length_1230_cov_2.880851, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000539 DAOQVW010000000
VERSION     DAOQVW010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162169_length_1230_cov_2.880851"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PKK85_09380"
     CDS             <1..899
                     /locus_tag="PKK85_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HNS26264.1"
                     /translation="DLEMMARGRESITSQDMDALAEKDTVHNIFDSVRTVLKSKNPRR
                     VKDALQLEAEPGFILELIAENVPREYENPEEIGRAYDRIADADVYLGRAFNTRHYGYW
                     KYAYDLMGPGVALSKKETYRKFARYTNSTMFSYLSKNRAKRDLRDRVAAKIGAKLHTS
                     RKVAIEQFPYLEIMFQDNVRAWELAQYFELEDGEVKQFRSRKIPQSVRKAAAMAKKES
                     ESSIGGGKTGKTSKKKGSSEDVVRKPTSKESKRKGKDSDKVSARKVPVPRAPEKNKTP
                     GNKSEVENASEKKEEQTSLFHF"
BASE COUNT          391 a          264 c          292 g          283 t
ORIGIN      
        1 acgacttgga gatgatggcc cggggtagag aatcaatcac ctcccaggac atggatgccc
       61 tggccgaaaa ggacaccgta cataacatct tcgactctgt gcgtactgtt ttgaagagta
      121 aaaatccccg ccgagtaaag gatgccctgc aactggaagc agaaccagga ttcattctgg
      181 aattaatagc tgaaaatgtt ccccgggaat atgagaaccc ggaagagatt ggaagggcct
      241 acgacaggat cgcagatgcc gatgtgtacc taggacgggc cttcaacacc cggcactacg
      301 gctactggaa gtatgcctac gatttaatgg gacccggagt ggctctttct aagaaggaaa
      361 cctaccggaa atttgcccgt tacaccaaca gcaccatgtt cagttatcta tccaagaatc
      421 gggctaaaag agatctgaga gacagggtgg ccgccaagat cggagccaaa ctccacacct
      481 cccgtaaggt ggccattgaa cagttcccct acttggagat aatgttccag gataatgtgc
      541 gggcttggga gttggcccag tacttcgagt tagaagatgg agaggtgaag cagttccgct
      601 cccggaagat accccagagt gtgaggaagg ctgcagctat ggctaaaaaa gaatcagaat
      661 cttcaattgg tggtggaaag actggaaaaa cctctaagaa aaaggggtca tctgaggatg
      721 tggtccggaa acccacctcc aaggagtcta aaaggaaggg gaaagattcg gataaggtct
      781 ctgccaggaa agttccggtg cccagggctc cagaaaaaaa caaaacaccc gggaacaagt
      841 cagaagtaga aaatgcatct gagaaaaaag aagaacagac ttctcttttc cacttttaat
      901 aaaagttgta gggtagggtc ccaggttcca agtcaagcca ggatatctgt gatcatcaac
      961 taaaaaaaaa gtgcatcaaa ttatccattt ggtctaaaaa taaagaagaa gtggggatat
     1021 aaattatcct attcttcagc atccatcatt tcgccgtaaa ctatttctat ttcacagcct
     1081 ttagggccgc agcagaagtg ttgtaggtca aattttactt gcattttttt cacctcaggt
     1141 aaaaattcac cgtcaatttt tacatgttca tatattataa tgtacctgac ggttaccaaa
     1201 ctatactact tcatggtata taaaaataac
//
LOCUS       DAOQVW010000540         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_164025_length_1222_cov_2.338475, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000540 DAOQVW010000000
VERSION     DAOQVW010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164025_length_1222_cov_2.338475"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1120
                     /locus_tag="PKK85_09385"
     CDS             191..1120
                     /locus_tag="PKK85_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2121 domain-containing protein"
                     /protein_id="HNS26265.1"
                     /translation="MSLIICYTGSNGSVIIGDKRRIGFFGNEKKRELLEEEFYSGSIK
                     TQEALLKRAEELGITLKITDDAEKVREIGDVITGEVKTTTPFETKRKRIYATTGSYSL
                     VELSGSTIKNMEGGTTSIVVFGNKYTKEIANKAIQDNWKKKVSLKDVGEIFEKAMDEV
                     SRQTPSVSPNYDLIIKHPSLNKKQARELLRTTILQDVKELEKWREELKEQMIKAAKGI
                     EMSNKILDNGVVGKVSKVEGHQVEIILAEGVEALDLEWNLQAEAGEVVAMEVDEPDKI
                     SIGDMAVIKDENLCIMPSQCGLKCEVILCKTDK"
BASE COUNT          432 a          197 c          302 g          291 t
ORIGIN      
        1 atttaaactc tattttagaa gaacagggat gataacctag gttttttgta ctctaaccag
       61 ttagatcctg tttttcattt ggattggaaa tcctattttg cactgtattt aaatgaatat
      121 ttttataaac ccagaatgga gatactctta atattagttg aaaaactaat tatttattgg
      181 tggaataaaa ttgagcctta taatttgtta cacaggaagt aacggcagcg ttataatagg
      241 agataaaagg agaataggtt tcttcggaaa tgaaaagaaa agggaacttt tagaggagga
      301 attttactcc ggatccatta aaacccagga ggctctgctt aaacgggctg aagaactggg
      361 aataaccctt aagataaccg atgatgccga gaaggtaagg gaaattggtg atgtaattac
      421 cggggaagtg aaaaccacca ctccctttga gacaaagagg aaaagaatat atgcaaccac
      481 cgggagttac agtttggtag aactatccgg ttcgaccata aagaacatgg aaggcggaac
      541 cacttctata gtggtgtttg gaaataagta caccaaagaa atagctaata aagccataca
      601 ggataactgg aagaagaagg tatccctgaa ggatgtgggg gagatatttg agaaggctat
      661 ggacgaagtt tccaggcaaa caccttcagt aagcccgaac tatgatctga tcatcaaaca
      721 cccctcctta aacaagaaac aggccagaga actccttaga accaccatac tccaggatgt
      781 taaggaactg gagaaatggc gtgaagaact gaaggaacag atgataaaag ccgctaaagg
      841 aatcgagatg tccaataaaa tcctggataa cggagtggta gggaaagtaa gtaaggttga
      901 gggccaccag gtggaaatca tcctggccga gggggtggaa gcactggatc tggaatggaa
      961 cctgcaggct gaagccgggg aagtggtggc catggaagtt gatgagccag acaaaatatc
     1021 cattggtgat atggccgtga tcaaggatga aaacctctgt atcatgccca gccagtgcgg
     1081 acttaaatgt gaagtcattt tatgtaagac agataaataa caattcagaa ttttaaggtc
     1141 taaattaaat tagggagaat aaatgaaact gacattttta ggaagtggcg ggggaagatt
     1201 tgccaccata acccagaaga gg
//
LOCUS       DAOQVW010000541         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_164312_length_1221_cov_1.658662, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000541 DAOQVW010000000
VERSION     DAOQVW010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164312_length_1221_cov_1.658662"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKK85_09390"
     CDS             <1..271
                     /locus_tag="PKK85_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26266.1"
                     /translation="VVTLSMFIIVIDKTFLNVAISTLITDLHTDIVTIQSIIAIYSLI
                     MASLMIPGGKLQDVLGRRKTFLLGAFIYGMGAVIASLSINSSMLL"
     gene            283..>1221
                     /locus_tag="PKK85_09395"
     CDS             283..>1221
                     /locus_tag="PKK85_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26267.1"
                     /translation="MEGMGAAFMMPATTSIISGTYSGERRAFALGIISSMASGAGTIG
                     PIIGGFLTTYYSWRYAFALELVVILAIFLLSREISSFPATIKWSDFDVAGALYSASGI
                     FLLVEGILLLNDPQTWNISLPMIIAGLILLSFFYFHQKRKISKGESALLDIALFNNRA
                     FSFGNIARLIMNFVIGGVLFVIPVFIQLILGIDPLTTGLTLIPMSLGIFALSFTAGKV
                     SSRFQARYILSVGFLVAMAGSIYLSLTFSPHTTILDMMPGILLLGVGMGIVFPHSTNV
                     IFAMARQDQQPDASGIMNTGINLGSAMGEWLSQNNIP"
BASE COUNT          276 a          268 c          264 g          413 t
ORIGIN      
        1 agttgttact ctctctatgt tcatcatcgt cattgataaa acttttttaa atgtggccat
       61 ttccacctta ataacggatt tacatacgga catcgttact atccagtcaa taatcgccat
      121 ttattctttg atcatggctt ctttaatgat acctggcggt aagctccagg atgttctggg
      181 acggaggaaa accttcctgt taggtgcatt tatttacggt atgggggctg ttattgcctc
      241 tttaagtatc aacagttcta tgcttcttta gggtggtctg tactggaggg tatgggggcg
      301 gctttcatga tgcctgccac tacctccatc atttctggaa cctatagtgg tgaaagaaga
      361 gcctttgcac tgggtataat cagttccatg gcatcaggag ccggtaccat aggccctatc
      421 atcggtggtt ttttaaccac ttattatagc tggcgttatg cctttgcact ggaactggtg
      481 gttatcctgg ctatattttt gctctcccgg gagatatcct cattcccggc taccatcaaa
      541 tggtctgatt ttgatgtggc cggtgcttta tattctgcct cggggatttt cctcctggtt
      601 gagggaatct tactcctcaa cgatccccaa acatggaaca tttcactgcc catgataatt
      661 gctggtttga tccttttatc ctttttctat ttccatcaga agaggaaaat cagtaaaggt
      721 gaatcagctt tgctggatat cgccctattc aataatcggg cttttagttt cggtaacata
      781 gccaggttga taatgaactt cgtaattggg ggtgtgcttt ttgttatccc cgtatttatt
      841 caactaattt taggcattga cccgctgaca actggtttaa cgttaattcc catgagcctt
      901 ggaatattcg ccctttcttt tactgcgggt aaggtatctt ccaggttcca ggcccgttac
      961 attctttctg taggattttt ggtggccatg gctggaagca tatatctaag tttaactttc
     1021 agtccccaca ctaccattct ggatatgatg cctggaattc tacttttagg tgttggtatg
     1081 ggtatcgttt tccctcactc taccaacgtg atatttgcca tggcccgtca ggatcagcag
     1141 cctgatgcct ccggtataat gaacaccgga ataaacctgg gttctgcaat gggagaatgg
     1201 ttatcccaaa ataacatacc g
//
LOCUS       DAOQVW010000542         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_165874_length_1214_cov_1.808456, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000542 DAOQVW010000000
VERSION     DAOQVW010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165874_length_1214_cov_1.808456"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..>1214)
                     /locus_tag="PKK85_09400"
     CDS             complement(203..>1214)
                     /locus_tag="PKK85_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS26268.1"
                     /translation="LGLEKEDYMVIIGPTGSGKSVLLETIAGFYRPQSGRIFLEGKDI
                     THLTPEKRGISIIYQDYVLFPHMNVFENIAYGLKKRIEDKEVIESSVVDAAKRLKIDH
                     LLDRNPETLSGGEKQRTAIARSLIVEPNLLLMDEPFSALDVNTHTYLTDLLKKAVSDY
                     QTTCIHVSHNFNDVWSLAEQVAVMRDGEILQQGVVADVFSKPRHDFVADFVGVRNIFQ
                     GEVVDGSKGITDVEIDKNLILHSSDTYNTDFKEVMVVIRPENIIFSNQPFVSSIRNQL
                     KGIVTEVVLIGPTVWVEVDVHGTSFKGIITPSSLDMLGIKKGNEIYLSFKSVNVKLID
                     ND"
BASE COUNT          344 a          259 c          217 g          394 t
ORIGIN      
        1 tatcaatcaa taatatacta tcatcaaatt tcaaacgata ttgtggccca tcttttgatc
       61 tgttcatgaa aatccctcta tcatatttaa ataatgacgg aaattagatt taataagtaa
      121 ttgtttatag tatatatttt cccaggttag gataaagttt tggaagaaaa ttagtcgcac
      181 cctaaaatta cgtggagtaa gatcaatcgt tatctattaa tttaacgtta acagatttaa
      241 aacttaaata aatttcgtta ccttttttaa ttcccagcat atccagtgaa ctgggtgtta
      301 ttattccttt aaagctagta ccatgtacat ctacttctac ccagacagtg gggcctataa
      361 gtacaacctc agtgactatg ccctttaact gatttctaat ggatgatacg aagggttgat
      421 tggaaaatat tatgttttca ggtcttatca ctaccattac ttctttaaaa tctgtattat
      481 atgtatctga gctgtgtaaa atcagatttt tatcaatttc tacatcggtg atgcccttac
      541 tcccatcaac cacctctccc tggaatatgt tgcgcacacc tacgaagtca gccacaaaat
      601 catgacgggg cttggagaaa acatcggcca ccacaccctg ctgtagaatt tccccgtctc
      661 tcatcaccgc cacctgctca gctaaactcc acacatcatt gaagttatgg ctcacatgta
      721 tacacgtcgt ttggtaatcg cttactgcct ttttaagtaa gtccgtaagg taggtgtggg
      781 tgtttacatc cagggcactg aacggttcat ccatcaacag aagatttggc tccacgatca
      841 gggatctggc tatggctgtg cgctgttttt ccccaccact cagggtttcc gggtttctat
      901 caagtaaatg atcaatttta aggcgtttgg ccgcatctac gacactggat tctataactt
      961 ctttatcttc aatcctcttt ttcagtccat aagctatgtt ttcaaataca ttcatatggg
     1021 gaaagaggac gtaatcctgg tagatgatgc tgatacctct tttttccggg gtaagatggg
     1081 tgatatcttt accttccagg aatattctac ctgattgggg acggtaaaaa ccagcgatag
     1141 tttctaaaag cactgacttt ccggagccgg taggcccaat aataaccatg taatcttctt
     1201 tttccaggcc aagg
//
LOCUS       DAOQVW010000543         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_166191_length_1212_cov_3.270527, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000543 DAOQVW010000000
VERSION     DAOQVW010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166191_length_1212_cov_3.270527"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..952)
                     /locus_tag="PKK85_09405"
     CDS             complement(230..952)
                     /locus_tag="PKK85_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNS26269.1"
                     /translation="MKNKKEYRIKIVKDGPYLVRGGVPLHEQKLVTDEAGHTKELIEV
                     KEHPSREIYSLCRCGASRDKPFCDGAHNEVGFDGTETADRKSYLDKAEVFEGPQLRLT
                     DAHEFCDHSRFCLRSGGIRELIKKSDDQAARKTAIEEATICPSGRLVLWDKETGEAFE
                     KEFEPSLVLIHDPQKNCEGPIWVRGGIPVESVDGTIYEVRNRMTLCRCGKSENKPYCD
                     GSHWMNAEQKLKFRRKWGLDEK"
     gene            complement(1011..>1212)
                     /locus_tag="PKK85_09410"
     CDS             complement(1011..>1212)
                     /locus_tag="PKK85_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HNS26270.1"
                     /translation="LDVDVEQAINRFEGKDHFENPEFLGKVRKNYLKLAQNHNFSVVN
                     ANHSVRKVHEDIKTILSSRIRI"
BASE COUNT          296 a          291 c          258 g          367 t
ORIGIN      
        1 acaaattaag aggattaata attaaaggag aataatgtga aaaaagccta ctagccgtat
       61 ttgatggctt gtctcttgga aacagggtct tgtcgagcga aatatgtaaa tccgtatgcc
      121 accatcttga aactggtgga ttccactaca gaaaaaaaag acttggggtg gagtttcatg
      181 gtacctggta ttgtagggaa attaacagtc aaacccagaa atcatatatc tatttttcat
      241 ccaaacccca tttcctgcgg aacttcagct tctgctcggc gttcatccag tggctaccat
      301 cacagtatgg cttgttctcg gatttaccac acctacaaag tgtcatgcgg ttacggactt
      361 cataaatcgt tccatccacg gattccactg gaatacctcc ccgtacccag ataggtcctt
      421 cacagttctt ctggggatca tggatcagga ctagggatgg ttcaaactcc ttctcaaatg
      481 cctcgccggt ttccttatcc caaagaacca gtctacctga ggggcagatg gtggcctctt
      541 caatggccgt ttttctggct gcctggtcat cggatttttt tataagttcc ctgatacccc
      601 ctgatctaag gcagaacctg gagtggtcgc agaactcatg ggcatcagta aggcgtaact
      661 ggggtccttc aaagacctcg gccttatcca ggtaggactt tcggtccgca gtttcggtac
      721 catcaaagcc aacttcattg tgggcgccgt cacagaaggg cttatcccgg gatgctccac
      781 agcggcagag actgtaaatt tcgcgactgg gatgttcttt tacctcaatt agctctttag
      841 tgtggccggc ttcatcggtg accagtttct gttcatggag cggcactccc cccctaacca
      901 ggtagggacc gtcttttact attttaatcc ggtattcctt tttattcttc atcttgaaac
      961 cctcatacta tagttgatac gtttcatgtc ccctatcccc tattttagat tcatattctt
     1021 attcgggagg atagtatggt cttaatatct tcatgaacct ttctaacgct atggttggcg
     1081 ttgaccactg aaaagttgtg attctgggcc aatttcaggt aattttttct gactttccct
     1141 aaaaattcgg ggttttcgaa gtgatccttt ccctcaaatc gattgatagc ttgttcaaca
     1201 tccacatcaa gg
//
LOCUS       DAOQVW010000544         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_166356_length_1212_cov_1.685393, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000544 DAOQVW010000000
VERSION     DAOQVW010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166356_length_1212_cov_1.685393"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..616)
                     /locus_tag="PKK85_09415"
     CDS             complement(20..616)
                     /locus_tag="PKK85_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HNS26271.1"
                     /translation="MNCTVCGSDSLEIIKAKGKKSKELLLKCEECGNIFREVVDVGKP
                     VDRRVVVSEFENSHKTFIKLYEDEILHLGDLIDLDGREAEITSLENKRGGRVSKSAVE
                     DVETIWASYTDIPARVGISIDYGGRILSQKVEVNPDFEFTIGEVVKMGNIIFRINSLK
                     TLERKMRKGFARASVTKRVYGRPVDEHRFKYDLTSKVV"
     gene            complement(721..1206)
                     /gene="hacB"
                     /locus_tag="PKK85_09420"
     CDS             complement(721..1206)
                     /gene="hacB"
                     /locus_tag="PKK85_09420"
                     /EC_number="4.2.1.114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitase small subunit"
                     /protein_id="HNS26272.1"
                     /translation="MDRIEGKVWTFRDSIDTDVIIPGRYLRAQTLDELASHVMEPEDP
                     TFAEKVQQGDVIVAGWNFGCGSSREQAPVALKHAGIAAIVAKSFARIFYRNAINIGLP
                     VIVANMEAEKGDEICIDLKKGIIEDKSTHKTFKIQPFKKFMLEILENGGLTEHYLKKQ
                     E"
BASE COUNT          289 a          308 c          219 g          396 t
ORIGIN      
        1 ccttcctttc cttctccatc tacaccacct tagaggttaa atcatactta aatctatgtt
       61 catcaactgg cctgccatac accctcttgg ttaccgatgc ccgggcgaat ccctttctca
      121 tcttcctctc caatgtcttc agggagttga tccggaagat gatgtttccc atcttcacca
      181 cctcaccgat ggtaaattca aagtcgggat ttacttccac cttctgggat aatattcgac
      241 ctccgtaatc gattgaaata ccaacccttg ccggtatgtc cgtataagaa gcccatatag
      301 tttcaacatc ctccactgca ctttttgaaa ctcggcctcc ccgtttattt tcaagggagg
      361 tgatctcggc ttcccttcca tccaagtcta tcaggtctcc gaggtggagg atttcatctt
      421 catacagttt gatgaaggtt ttgtggctgt tttcaaattc actcaccact acccggcgat
      481 ctacgggttt acccacatct accacttctc tgaagatgtt tccacattct tcacatttaa
      541 ggagaagttc tttggatttt ttacccttag ctttaattat ctccagggaa tcggagccac
      601 atacagtaca gttcatattt ttaatttcct ctattttagt aatgatattt gttttcaata
      661 aataaaattc ttagtaaaaa aaatgtagaa gtacattaga tcaataaaaa taaatgggga
      721 ttattcctgt tttttaagat aatgctcggt caaaccgccg ttttccagga tttccagcat
      781 gaatttcttg aagggctgta tcttgaaggt tttatgggtg gatttatcct ctataattcc
      841 cttcttcagg tctatacata tctcatcacc cttctctgcc tccatgtttg ccacgatcac
      901 tggcaggcca atatttatgg cgtttctgta gaatatccgg gcaaatgatt tagctacaat
      961 tgcagctatc ccggcgtgtt tcagggccac cggggcctgc tcccgggatg aaccgcagcc
     1021 gaaattccag ccagccacta tgacatcccc ctgttgcact ttctctgcaa atgtggggtc
     1081 ttctggttcc atgacgtgtg atgccagttc atccagtgtt tgggccctta agtatctgcc
     1141 tggaatgatc acatcggtgt ctatgctatc ccggaaggtc cagacctttc cttctatcct
     1201 atccatcata at
//
LOCUS       DAOQVW010000545         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_166438_length_1211_cov_3.293253, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000545 DAOQVW010000000
VERSION     DAOQVW010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166438_length_1211_cov_3.293253"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKK85_09425"
     CDS             <1..221
                     /locus_tag="PKK85_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS26273.1"
                     /translation="IFEAPGCQEIKFKKILAEGDPAEEIVKTAELEGVDLIILGTGKN
                     IVDKRLLGSVSEKVVHSTPCTILLVKTT"
     gene            346..>1211
                     /locus_tag="PKK85_09430"
     CDS             346..>1211
                     /locus_tag="PKK85_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HNS26274.1"
                     /translation="MSLFKKILGIGPKPVMAKSKYITIEDAKMATFTKKAVGQGYGTS
                     LSPDVYYIVASVELGNTTTKCILTATNLNTCRTYLLDKTVKMTRDIRPPKKGEKVFGE
                     TVWHIELTRESVSEMVRDTILESIKRSNIDIEKDLDFVVRSTGVTAGFASLEEVGELI
                     IALADGCLDAGIPPRKMSPAISKEALPKKLREFTLLDKVMFDGAVVSVIPPTGRSVVA
                     NEMEGELVTAGIKAGAKWTEIDFRNPCVSMDFGTTLAGRIVNNDQPYARTIGNFCGLS
                     GAISDAVIRGTE"
BASE COUNT          385 a          234 c          282 g          310 t
ORIGIN      
        1 aaatctttga agctccaggt tgccaagaaa taaagtttaa aaaaatccta gccgaaggag
       61 atccagccga agaaattgtt aaaactgcag agcttgaagg agtagatcta ataatactgg
      121 gaaccggtaa aaacattgtt gataagaggc ttttaggaag tgtttctgaa aaagttgttc
      181 actcaactcc ttgcactatc ttactggtga aaacgacatg aaatgttttg tagaacactt
      241 ttttaatttt atattcaatt tagaggggca actttttttt cacaagattt ataactattt
      301 taaataaatc ctaatctgga gacttcatat aaggagggga ttattttgtc actttttaag
      361 aaaattctag gtataggtcc caaaccagtt atggcaaaaa gtaagtacat taccatagaa
      421 gacgctaaga tggccacctt taccaagaaa gcggtgggcc agggttacgg aacctccctg
      481 agcccggatg tctactacat cgttgcctcg gtggaacttg gaaacaccac cactaagtgc
      541 atcttaactg ccaccaacct taacacctgt cgcacctacc tcctggacaa gacagtgaag
      601 atgaccaggg atattaggcc tcccaagaag ggtgaaaagg tatttggaga gacagtctgg
      661 catatagaac tcaccagaga atctgtatcc gaaatggtac gggataccat attggagtcc
      721 attaaacgtt ctaacattga tatagaaaag gatttagatt ttgtggtccg atcaactgga
      781 gtaactgctg gtttcgcatc cctagaagaa gtgggggagc tgatcatagc cctggcagat
      841 ggatgtctgg atgctggaat accaccacgt aagatgtctc cagccatatc aaaagaggct
      901 ttacctaaaa aactacggga attcacgtta ctggataagg tgatgttcga tggggctgta
      961 gtaagcgtta taccacccac gggtcgttca gtagtggcta acgagatgga gggagaactg
     1021 gtaactgcgg gtataaaagc aggagcgaag tggacagaaa tagatttcag aaacccctgt
     1081 gtatctatgg acttcggaac aaccttagct ggccgtatag tcaataatga tcagccatat
     1141 gcccgaacca taggaaactt ctgtggactg tctggagcca tttcagacgc agttatccgg
     1201 ggaacagaga a
//
LOCUS       DAOQVW010000546         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_167011_length_1209_cov_2.454073, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000546 DAOQVW010000000
VERSION     DAOQVW010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167011_length_1209_cov_2.454073"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..542
                     /locus_tag="PKK85_09435"
     CDS             150..542
                     /locus_tag="PKK85_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNS26275.1"
                     /translation="MDDEKIFKIALLTSLVGLVGMIFTAGYIAPQKVRIEDINLGMID
                     KEVSVEGFVQSVKKSRNGDTYFLDVVDASGRLTIVIFQSTAHDLEKNDINITGLNQRR
                     VNVVGNVAEYHGTLELIMKDSQSLKILA"
     gene            685..>1209
                     /locus_tag="PKK85_09440"
     CDS             685..>1209
                     /locus_tag="PKK85_09440"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646025.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNS26276.1"
                     /translation="MKTKRLFGTFGVRRIANEVLTPEFASKLASAYGSVVKGKVAVGG
                     DPRTSTPMIKHAVIAGLLSSGCQVVDLGILPTPTVQYAVRNYYDGGVIITASHNPSQY
                     NGIKFVDENGIGIKEEMEIQIEDIFFDETPDRVSWDELGDVSSLPGIVEEYIEHVIHR
                     VDEKAIDQAKLKVIV"
BASE COUNT          379 a          195 c          278 g          357 t
ORIGIN      
        1 taagggcata cgttatgatt taagtcccgt ggatgttgat actctggata gaattgacgc
       61 catgtttaag ggctcccaga aatcctgaca attttttacc aacccaaaat attaatacta
      121 ctgattcaga aattattact ggtgttggtg tggacgatga aaagatattt aaaattgccc
      181 ttttaacctc cctagtggga ttggtgggaa tgatattcac agcaggatat atagcccccc
      241 agaaggttcg gatagaggac attaatctgg gaatgattga caaagaagta tcggtggagg
      301 gttttgtgca gagtgtgaaa aaatccagaa acggagatac atatttcttg gatgtggtag
      361 atgctagtgg caggctaacc atagtaattt tccagtccac agcacatgat ctggaaaaaa
      421 atgatatcaa cattacaggg cttaatcaaa ggagggtaaa cgttgttgga aacgttgcgg
      481 aataccatgg caccttggaa cttataatga aggactctca atccctgaaa atcctggctt
      541 gaactatcag tgtagttcta gttccacttt aacgatgcga tgttgggaat acataggcaa
      601 attatatcca aatagactat ataattagta tagatattgt taaggatttt cttaaaaaag
      661 atgcacaaaa accggggtat ttttatgaaa actaaaagat tattcggcac atttggggtt
      721 agaaggatag ctaatgaggt attaactcct gaatttgctt ctaaattggc ttctgcctat
      781 ggttctgtag ttaaaggtaa agttgccgtg gggggcgatc ctaggacctc cactcctatg
      841 atcaagcatg cggtgattgc tgggctttta tcttccggat gtcaagttgt tgatctagga
      901 attctgccaa ctcctactgt tcagtatgct gtacggaact actatgatgg tggagtcata
      961 attacagcct cacacaatcc ttcacagtat aacggaatca aatttgtgga tgaaaatggt
     1021 ataggaatta aagaagaaat ggaaattcaa attgaggaca tattttttga tgaaacacct
     1081 gatagggtct catgggatga acttggcgat gtatcctctc tcccgggtat tgtagaggaa
     1141 tatatagaac atgtaattca tcgggtggat gaaaaagcca ttgatcaggc taagcttaaa
     1201 gttattgtg
//
LOCUS       DAOQVW010000547         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_167160_length_1208_cov_3.776236, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000547 DAOQVW010000000
VERSION     DAOQVW010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167160_length_1208_cov_3.776236"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1160)
                     /locus_tag="PKK85_09445"
     CDS             complement(24..1160)
                     /locus_tag="PKK85_09445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNS26277.1"
                     /translation="MDDVPRPNYVCIFPHLYNFQLIKDVGMIPFTMGRLFNYHASIVT
                     YANDDYSYMDKDLKDENFELDFIKKRFNNHNYDIFLYLICNSRSIDVLQVFHFHDTLN
                     ILFYFLIYKSLNWNGKIYAKLDADYNLTKFLAEDKGFWPGIRNFILKHLVDVLSVETA
                     ESYQMLLDSKLKYHGKLIHMPNGVDIHVTPELMVDDKKEYILTVGRLGSEEKATEVLL
                     EAFSKIKDHKNWKLILVGKFEHQFQDYVNEYFQIHPHLREKVIFTGFIEDRNKIYRYY
                     AQSKIFCFTSRSESFGIALVEAAYFGDYLVSTNVGGAQDVLKVTDYGELFETDNIPIL
                     VDRLEDLISNWHKYEADPRQKMNLMEENFSWPHLCEKLYKKLNE"
BASE COUNT          355 a          247 c          217 g          389 t
ORIGIN      
        1 cttcattaaa tcacttgtcc tgtctattca tttaattttt tataaagttt ttcacataaa
       61 tggggccaac taaaattttc ttccatcaaa ttcatcttct gcctgggatc agcctcatat
      121 ttgtgccagt tacttatcaa atcctctagt ctatcaacta agattgggat gttatctgtt
      181 tcaaacaatt caccataatc tgttacctta agaacatcct gagcccctcc cacattggta
      241 gaaactaggt aatccccgaa atatgcagct tcaaccaggg cgattccgaa actctcgctc
      301 cgggaagtaa aacagaatat cttggactgg gcatagtacc ggtaaatttt gttccgatcc
      361 tcaataaaac cagtgaaaat aactttttcc cgtagatggg gatggatttg gaagtattcg
      421 ttaacataat cctgaaattg atgctcaaat ttaccaacca gaatcaattt ccagttttta
      481 tgatccttga ttttagaaaa agcctccaac aacacttcag tagctttttc ttcggatccc
      541 agcctcccca ctgtcagtat gtactctttc ttatcatcca ccatgagttc aggggtaacg
      601 tgtatgtcca cgccattggg catgtggatt agtttaccat gatactttaa cttactatca
      661 aggagcatct ggtagctttc tgcagtttcc acactcaaaa catcaaccag atgtttaagt
      721 ataaaattac gtatacccgg ccagaagccc ttatcttcgg ctagaaattt ggttaaattg
      781 taatccgcat ccaactttgc atatatttta ccattccagt tcagggactt gtaaattaaa
      841 aaatagaata gaatgttcag tgtatcgtga aagtggaaaa cctgcagtac atctatgctt
      901 ctggagttac agatcaggta gaggaagatg tcgtagttat gattgttaaa tctcttttta
      961 atgaaatcta actcgaagtt ttcgtctttc aggtccttat ccatgtagct gtaatcatcg
     1021 ttggcgtagg tcacgatgga tgcatggtaa ttgaacaatc tgcccatggt aaaggggatc
     1081 atacccacat ccttaattaa ctggaagtta tacagatggg gaaatataca tacgtagttg
     1141 ggtcttggta catcatccaa cagaatcaca tcatcttttt ttagtgttga agatagtggt
     1201 taatgggg
//
LOCUS       DAOQVW010000548         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_167912_length_1205_cov_2.613043, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000548 DAOQVW010000000
VERSION     DAOQVW010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167912_length_1205_cov_2.613043"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1205
                     /locus_tag="PKK85_09450"
     CDS             <1..>1205
                     /locus_tag="PKK85_09450"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS26278.1"
                     /translation="RLVVDSAGEVDESDESNNEYTRYLILNEAGVVRVFIISDNPGTN
                     IVNQAAHEIMDMLGGAVSIQPRSNTQVEAMTEDQLLEYLNRCDIFIGEWISTNAATLL
                     SKVLTTHPMVANKPNGVFLILEPPVSTISTSVELMKYSNIRGVRILDGFTNDQLLDFY
                     KNTLRGSNYTAMMDYLSTVNFPLDYETATQYKNLNDKDNLKNQILWALNLIGVESSYQ
                     APTLSSEKKEYGIYRYRWYTLSDYMATYFEGTRKGTVGVIESTMYVDSQMLQTYYGII
                     DSLEAQGLNVIPVMAYGATTAQLNIMVQAFTNAPDYATFMANPEQYTVYVDSIVEMAA
                     YGLGGESFNDVINFFKVLNVPVIRAIHSDVITNEEWELESTGLPTADGSKWWHISILE
                     AQGIIEYNFV"
BASE COUNT          320 a          312 c          309 g          264 t
ORIGIN      
        1 cgcttggtgg tggactctgc cggggaggtg gacgaatccg atgaaagcaa caacgagtat
       61 acccgttacc tgatcctcaa tgaagccggg gtggtgaggg tgttcatcat cagtgacaac
      121 cccgggacta acattgtgaa ccaggctgcc catgagataa tggatatgct gggtggtgct
      181 gtgtccatcc agccccgcag caacacccag gtggaggcta tgaccgaaga tcagctgttg
      241 gagtacctga accgttgcga tatattcatt ggagagtgga tcagcacaaa cgcagcgact
      301 cttttatcca aagtcttaac tacccatcct atggtggcaa ataaacctaa tggagtgttt
      361 ctcatcctgg agccaccagt ctccaccatc agtaccagtg tggagcttat gaagtactcc
      421 aacatccgtg gagtgcgaat cctggatggc ttcaccaatg accagctcct ggatttctac
      481 aaaaacaccc tgcggggcag taactacacg gcaatgatgg attacctctc cactgtgaac
      541 tttcccctgg actatgaaac cgccacccag tacaaaaacc tcaatgacaa ggataacctg
      601 aaaaaccaga tactctgggc cctgaatctt attggggtgg agagcagtta ccaggcacct
      661 accttgtcct cggagaagaa agagtacggt atctatcgct accggtggta taccctcagt
      721 gactacatgg ccacctactt tgaaggaacc aggaagggta cggtgggagt catagagagt
      781 accatgtacg tggacagcca gatgctccaa acctactacg gcataattga ctccttggag
      841 gcccagggac ttaacgtaat cccagtcatg gcctacgggg ccaccactgc ccagctaaac
      901 atcatggtcc aggccttcac caacgccccg gactatgcca ctttcatggc caatccagaa
      961 cagtacacgg tgtacgtgga tagtatcgtg gagatggctg cttatggcct gggtggggag
     1021 agtttcaacg atgtgatcaa cttctttaaa gtactgaacg tgccggtgat ccgggccatc
     1081 cactccgatg taattaccaa cgaagaatgg gagctggaaa gcactggact gcccactgct
     1141 gatgggagta aatggtggca catctccatc ctggaggccc agggcataat tgaatacaac
     1201 ttcgt
//
LOCUS       DAOQVW010000549         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_168526_length_1203_cov_1.950348, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000549 DAOQVW010000000
VERSION     DAOQVW010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168526_length_1203_cov_1.950348"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PKK85_09455"
     CDS             <1..1200
                     /locus_tag="PKK85_09455"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS26279.1"
                     /translation="GFVIVSHLPPTMVPSDLYGDLSALQQKITLYNQVQEEGALKEEY
                     KQTIISECKRLGLDQDLNVDLSTLNDTTAFKNFIQELDEYLNEIKVSYIPYGLHILGQ
                     APTNESLVAMVESMFGDSFQKDVAAINSKQGLTTKLLIEVLLNGLSPEKAQNKVLGQI
                     SSKITAYLELAIDYRDLINESARMEISELLNALNGQYIQPSIFGDLVGRPELLPTGKN
                     LYSFDGRLMPTKEAWDIAVELVEQQLQEYKENHNNTYPDKIAMVLWKSETCRHQGVME
                     AEILYLLGVRPVWDKNNRWSDVELIPSSELGRPRIDVIITTSGSYRDMFGYKFELLEK
                     AVKLAASANDIEYPNYVKEHSDALYQWLIENGYNATEAKDLSTARIFSHEVGRFGSKL
                     FETPTTL"
BASE COUNT          412 a          242 c          255 g          294 t
ORIGIN      
        1 ggttttgtta ttgtaagcca cttaccccca accatggttc catcggacct gtatggagat
       61 ttatccgcat tacaacaaaa aatcaccctg tacaatcagg ttcaggaaga aggtgcactg
      121 aaagaggagt acaaacagac cataattagc gaatgtaaga gattaggcct tgatcaggat
      181 ctgaatgttg atctttcaac actcaacgat accacggctt tcaaaaattt cattcaggaa
      241 ctggatgaat atctcaatga aattaaagtc agttacatac cgtatggttt gcacatactg
      301 ggccaggccc ctaccaatga aagcctggtg gcaatggtgg aatccatgtt tggagattcc
      361 ttccagaaag atgttgctgc cataaactca aagcagggat taactacgaa gttactgata
      421 gaagtcctgc ttaatggcct gagcccggaa aaggcacaaa acaaagtgtt aggacaaata
      481 tcatctaaaa tcacagcata cctggaactt gccattgatt acagggattt gataaatgaa
      541 agtgcccgaa tggagatctc agaattactt aatgcactga atggacagta catacaaccc
      601 agtatattcg gtgatctggt tggtcgtcca gaacttttac caacaggtaa aaatctctac
      661 agctttgatg gacgacttat gcctacaaag gaagcatggg acattgcagt agaactggtt
      721 gaacagcaac tacaggaata caaagaaaac cacaacaaca cttaccctga taagatagca
      781 atggtactct ggaaatcaga aacatgtaga caccagggtg tgatggaagc agaaatactg
      841 tatcttttag gtgtacgacc cgtatgggat aaaaacaaca gatggtctga tgttgagcta
      901 attccctcca gtgaattagg ccgaccacga attgacgtga taataactac cagtggatct
      961 tacagagaca tgttcggcta taaattcgaa ctgttggaga aggctgtgaa attggcagcc
     1021 agtgctaacg atattgaata tccaaactat gtcaaggagc acagtgacgc actttatcaa
     1081 tggttaatag aaaatggata caatgcaact gaagcaaaag acctctccac tgcccgaata
     1141 ttctctcacg aagttggcag atttggatca aaacttttcg aaacgccaac aacattatag
     1201 aaa
//
LOCUS       DAOQVW010000550         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_168670_length_1202_cov_2.769834, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000550 DAOQVW010000000
VERSION     DAOQVW010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168670_length_1202_cov_2.769834"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKK85_09460"
     CDS             complement(<1..468)
                     /locus_tag="PKK85_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26280.1"
                     /translation="MEQEPNTPAQEMNKTVVLLIATLATFLTPFMGTSLIIALPSIGN
                     ELAVNAILLSWITTAYFLTSAMFAVPFGRIADIYGMKRIFTYGIIILTISTFLAAISP
                     SAEFLVVTRAFQGVASAMIFVTALAIITSVYPPQERGKAIGINITAGYAGLVMG"
     gene            566..>1202
                     /locus_tag="PKK85_09465"
     CDS             566..>1202
                     /locus_tag="PKK85_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNS26281.1"
                     /translation="MNYKEEAHAFKKIFKLKYEPIAISFTNDDVSLGRYEKTSICRAL
                     KHASSGESFLIDKTVSICPGGSQYCGFSPLHAGKKKRRVQKFLTKGEKLTSSIVTFER
                     MQRLAIPPATDLADRILIGPLERAEIRPDMILFLCNGEQACRLITLDTYWDGISPEQQ
                     IIGALCYSAIVYTIMSGNTNMSLGDWTARSHQKFEPDVIFLSVPYERIDNLL"
BASE COUNT          368 a          240 c          274 g          320 t
ORIGIN      
        1 ccccataacc agcccggcat aaccagcggt gatatttatc cctatggctt tacccctttc
       61 ctggggtggg tagacagatg ttattatggc cagggcagtt acaaatatca tggcactggc
      121 aacaccctgg aaggccctgg tcactaccag gaactcagct gatggggata tggcagccag
      181 aaatgtggag atggttaata ttataatacc ataggtgaaa atccttttca tcccatatat
      241 atccgcgatt cggccaaagg gaacggcaaa catggctgat gttaaaaaat atgcggtggt
      301 gatccagctt aaaaggatag cgttaacagc taattcattg cctatgcttg gaagagctat
      361 gatcagggaa gtgcccatga atggggttaa gaatgtggcc agggtagcaa tcagtaaaac
      421 tactgttttg ttcatttctt gggccggggt gttcggctcc tgctccatct ttgctcccat
      481 atatataaaa taaatggttt catttataaa tatagcagtt acaacctaga aggtccattt
      541 gaatttccca ggaggggttg gtagaatgaa ctataaagaa gaggcccatg cttttaaaaa
      601 gatttttaaa ttaaaatatg aaccaatagc tatttctttt acaaacgacg atgtttcact
      661 aggcagatat gaaaaaacaa gcatatgtag ggcattaaaa catgctagta gtggagaaag
      721 ttttctaatt gataaaactg tctctatctg ccctggtggt agtcaatact gtggtttcag
      781 cccacttcat gctgggaaaa agaagagaag ggtgcagaaa ttcctcacca agggggaaaa
      841 gcttaccagc tccattgtaa catttgagcg gatgcagagg ttagccatac ccccagcaac
      901 tgacctggca gatagaattc tgataggtcc cttagaaagg gcggaaattc gtccggacat
      961 gattttattc ctatgtaatg gtgaacaggc ttgtcgccta ataacattgg acacctactg
     1021 ggatggcata tcccctgaac agcaaataat aggtgcatta tgctattcag ctatagtcta
     1081 caccataatg agtggaaaca ccaatatgtc cctgggagat tggactgcca ggagccacca
     1141 gaagtttgaa ccagatgtca tattcttatc agtaccctac gaacgcatag ataatctgtt
     1201 aa
//
LOCUS       DAOQVW010000551         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_169009_length_1201_cov_1.835951, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000551 DAOQVW010000000
VERSION     DAOQVW010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169009_length_1201_cov_1.835951"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1201)
                     /locus_tag="PKK85_09470"
     CDS             complement(<1..>1201)
                     /locus_tag="PKK85_09470"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS26282.1"
                     /translation="MYITSQKLQTYYAIVDSLEAKNLNVLPVFAAGATPNQLQIMVET
                     FTSAPNFDSFIANPSKYKVYVDSIVEMTAYGVGGSEFGNVTKFFSALNVPVVRAIHSD
                     VMTNEEWELANTGLPTDDGSKWWHVAILEAQGIIEYSFVGGTSVKIDPDTGAQIEGYT
                     PQTENIEFMNKRIASWVNLQNMKNSDKLISLIYYNYPPGKNNVGSSYLDTITSIYNLL
                     KILKNAGYTVKDLPASEDDLLDMIIGDQEKGILPLGINVANWAQGELDKLASNPNIIL
                     YPVSQYNKWFSKLDKMTQLQVTEGPVAYIGEICKQAVKLKYTTEMATKIDNWYASVIA
                     LVPDDKYSKANPILKNIANSLKNYIKSGKSADYDSYLKYKNQFFKLNISGMNGWGKAP
                     GNVMVIKK"
BASE COUNT          262 a          256 c          270 g          413 t
ORIGIN      
        1 cttctttatc accatgacgt ttccgggtgc ttttccccat ccgttcattc cactgatgtt
       61 cagtttgaaa aactggtttt tgtacttgag ataggaatcg tagtctgctg atttacctga
      121 tttgatgtaa ttctttagac tgttggcgat gttcttcagt atgggatttg ccttactgta
      181 tttatcgtcg ggaacgagtg ctataactga agcataccag ttatctatct ttgtagccat
      241 ctctgtggtg tacttgagct tcactgcctg tttacatatc tctccaatat aagccaccgg
      301 tccttctgtc acctgtaact gggtcatttt atccagtttg gaaaaccatt tgttgtactg
      361 gctaactgga tatagtatga tgttcgggtt actggccagt ttatccagtt ctccttgagc
      421 ccagttagcc acgttgatac caaggggtag tatgcctttc tcttgatctc cgataatcat
      481 atccaggagg tcatcctcac ttgccggtag atctttaacg gtgtaacctg catttttcag
      541 gattttaagc aggttgtaga tgctggttat ggtgtccagg taactggatc ccacgttgtt
      601 ttttccgggt gggtaattgt agtatatcag ggagatcagt ttatccgagt ttttcatgtt
      661 ctgcaggtta acccaggatg ctattcgctt gttcatgaat tctatgtttt ctgtctgtgg
      721 ggtgtatccc tctatttgag ctccagtatc cgggtctatc tttaccgaag ttccacctac
      781 gaaactgtat tcgattattc cctgtgcttc cagtatggca acgtgccacc atttactccc
      841 atcatcagtg ggaagtccgg tgttagccag ttcccattct tcattggtca taacatccga
      901 gtgtatggcc cttacaacag gcacgttcaa tgcagagaag aacttagtta cattaccaaa
      961 ttctgaacct cctactccgt atgcggtcat ttccacaatg gaatccacat acactttgta
     1021 tttagagggg tttgcaatga aactgtcgaa attaggggcg ctggtgaagg tttccaccat
     1081 tatttgcagt tgattggggg ttgctcctgc agcaaagact ggtaagacgt taaggttctt
     1141 tgcttccagg gagtctacga tggcgtagta ggtctggagt ttctgtgaag ttatgtacat
     1201 g
//
LOCUS       DAOQVW010000552         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_169635_length_1198_cov_2.724409, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000552 DAOQVW010000000
VERSION     DAOQVW010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169635_length_1198_cov_2.724409"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKK85_09475"
     CDS             <1..295
                     /locus_tag="PKK85_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deacylase"
                     /protein_id="HNS26283.1"
                     /translation="STKPRGVPGKTSILCTRYPTYESYKMALSISQQTRNSLISQHQA
                     GASYPGAVEDVCNLAGIPAVTCEVKSRHNTVAPGSVTSSYNQMIALLRYMALL"
     gene            484..>1198
                     /locus_tag="PKK85_09480"
     CDS             484..>1198
                     /locus_tag="PKK85_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNS26284.1"
                     /translation="MDFKFKTKDDVTLVLGGEAGQGIQTVEQILLWAFKMAGYNVCAS
                     KEYMSRVRGGQNSTQIRVSSRRVSAYKEHTDLLVALSPGALEHLKNRIYPDTVVMGDE
                     KDVEYPALGLPFLKLAQEIGSPIYANIIAAGAVCNLFQVDADIFNQCLTAMFSRKGEE
                     ILQKDIQAGREGYQMAESWGKVEVERDPSVADQVLLNGSEAVALGCMSGGCKFIASYP
                     MTPSTPVQVYMAEHGREVGV"
BASE COUNT          322 a          275 c          326 g          275 t
ORIGIN      
        1 ctccacaaaa ccgagaggtg ttccaggaaa aaccagcatc ctctgtacca ggtatcccac
       61 ctacgaaagc tacaagatgg ccttgtccat tagtcaacaa accaggaaca gtttaattag
      121 ccaacaccaa gccggagcat cctatccagg agcagtggaa gatgtgtgta acttagcagg
      181 aatccctgca gttacatgtg aagttaaatc cagacataat acagtggcac ctggcagtgt
      241 aacaagctcc tacaatcaga tgatagcact cttaaggtat atggccttac tataaaggct
      301 taactagaat ggggttacca aaactttttt tttacatcca tatccgtcta gagagaaacc
      361 tggataccac cttcactatc catatcacta agtaatggac tgaacaaata tattatacac
      421 atcattggtg gttcgatatt ctccagttga atcttaagcc actggatgtg gggggttttt
      481 tgtatggatt ttaagttcaa aactaaggat gatgtgactc tggtcctggg gggtgaggct
      541 ggtcagggaa tccagaccgt ggagcagata ctcctgtggg cctttaaaat ggcgggatac
      601 aatgtctgcg cctctaagga gtacatgtcc cgggtgcggg gtggacagaa ctccacccag
      661 atcagggtgt ccagtaggag ggtttcagcc tacaaggaac acaccgacct cttggtggcc
      721 ctgagccccg gggccctgga gcaccttaag aaccggatat atccggatac cgtggttatg
      781 ggtgatgaga aggacgtgga atatcctgcc ctgggtttgc cctttttgaa actggcccag
      841 gaaataggga gccccatcta tgccaacatc atcgccgccg gggcggtgtg caacctgttc
      901 caggtggatg ctgatatatt caaccagtgc ctgactgcca tgttcagccg gaagggggag
      961 gagatactgc agaaggatat tcaggctgga agggagggct accagatggc tgagtcctgg
     1021 ggtaaagtag aagtagaaag ggacccctca gtagcagacc aggtgctcct taatggtagt
     1081 gaagccgtgg cactgggctg tatgagtggg ggctgtaaat tcatagcctc ctaccccatg
     1141 accccctcca caccagtgca ggtctacatg gcagagcatg gaagagaggt gggggtgg
//
LOCUS       DAOQVW010000553         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_170076_length_1196_cov_3.275197, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000553 DAOQVW010000000
VERSION     DAOQVW010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170076_length_1196_cov_3.275197"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKK85_09485"
     CDS             <1..467
                     /locus_tag="PKK85_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="geranylgeranyl reductase"
                     /protein_id="HNS26285.1"
                     /translation="LKKYHGCIPIFNPKKQIVKKRAILVGDAASQVKPTTGGGLTLGF
                     LCAKLASEVMVKALEIENTEILQKYQTLYGKKFGKELKTQRMVHKTFQSLNDAELDFM
                     FSKLKEEDAEDLISEYGDMDTQSPLVKEMLRRGIIFSILPQLLSRRISSLWK"
     gene            458..>1196
                     /locus_tag="PKK85_09490"
     CDS             458..>1196
                     /locus_tag="PKK85_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNS26286.1"
                     /translation="MEIALIPSLEHPTLPRAEVEAVFHAEKYKYSFKKVKKGLIVIEV
                     PNRLFQELPNTVRRLSFTHEVFRVLLHTSSEKLLEKAAEYPWEDVVQRDYAVRVRKID
                     NKSDLNSLDLEKKLGGIIKRRTGARVNLKNPHVLIRTVLNDDQVLVGDRVSKISKKHF
                     FEFKPHKRPFFYPGSMHPKLARCMVNLTHVKRGESLLDPFCGTGGILIEAGIIGAQVI
                     GSDLDYKMVKGTKENLLHCGITDFKIFR"
BASE COUNT          387 a          234 c          269 g          306 t
ORIGIN      
        1 acctaaaaaa atatcatggt tgtatcccaa ttttcaaccc aaaaaagcag attgtaaaaa
       61 agagggccat ccttgtgggg gacgcggcat cccaggtgaa gcccaccaca gggggtggtt
      121 tgactctggg ttttctctgt gctaaattag catcagaggt aatggtcaaa gcattggaga
      181 tagaaaatac ggagattctg cagaaatatc aaacattata tggtaaaaag tttggaaaag
      241 aacttaaaac tcaacgaatg gtccataaaa cttttcaatc tcttaatgat gcagaactgg
      301 acttcatgtt ctccaaactt aaggaagaag atgctgaaga tctgatatct gaatatggag
      361 acatggacac ccaatcaccc ctagtaaagg agatgcttcg aaggggaatc atattttcca
      421 tactccccca gctactgtct aggaggattt cttctttatg gaaatagctt tgatcccctc
      481 cctggaacat cccactctgc ccagggccga agtggaagca gttttccatg cagaaaaata
      541 taaatacagt tttaagaagg taaaaaaagg cttaatagtt atagaagtgc ctaatagact
      601 ttttcaggaa ctccctaata cagtaagaag actatcattc acccatgaag ttttccgggt
      661 gttgttacac acttccagtg aaaaattact ggaaaaagca gctgaatatc cctgggagga
      721 tgtcgtccaa cgggattatg ccgttagagt caggaaaatt gataataaat ccgatttgaa
      781 ctctttagat ctggagaaaa aactgggcgg aattattaaa aggcgtaccg gagcccgggt
      841 caacctgaaa aacccacacg tcctcatcag aacggtgtta aatgatgacc aagtcctggt
      901 gggggatcgt gtatctaaga tatcaaaaaa acatttcttt gaatttaaac cccataaaag
      961 accatttttc taccccggat ccatgcatcc caagctggcc cggtgtatgg tgaacctgac
     1021 ccatgttaag cggggggaga gtttactgga tcccttctgt ggaacaggtg ggattctaat
     1081 tgaggccggg atcatcgggg cccaggtgat aggcagtgat ttggattata aaatggtaaa
     1141 aggtaccaag gaaaacctat tgcactgtgg gataactgat ttcaagatat tcagat
//
LOCUS       DAOQVW010000554         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_170160_length_1196_cov_2.302366, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000554 DAOQVW010000000
VERSION     DAOQVW010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170160_length_1196_cov_2.302366"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..>1196)
                     /locus_tag="PKK85_09495"
     CDS             complement(422..>1196)
                     /locus_tag="PKK85_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26287.1"
                     /translation="MLSTKELLECDYCGAVFEKKGSKYKLKDIYDKGCQFWQRYRGKI
                     LTEEEWTRIAKGGISNEEQRIIEEKERKKQQLIEEQTAQNDLVTFVNQLAQGTVNLTP
                     LSNPPIILKKKEVAHLVLGNISFHEPRAVRKTYGGYAGPTIRVTKGVSFRVGSVAARS
                     ESHEELRKIDQGTLVLTNKRLIFTGSKRTINIDLRKIIAIEAYKDGVASQRENKQKTE
                     YFIGSNRSTLTFTTNGRRHTVPFTGPILKAAIQGQISQT"
BASE COUNT          278 a          258 c          276 g          384 t
ORIGIN      
        1 ggtcgatgta ccgcttcatg cgggggcgta gtttgttgag catgattttt ttaccaaagt
       61 tagtttacag caacactatc tgattagaaa tctgaagtta tttaaaccta tcttggaatg
      121 gagaataaaa aattggtgta taattgttta aattctttta agatgaatct taaagctatt
      181 cataaatatt tacagtaatt gattgattat tctactattg tcttctggag gcccaagtac
      241 gatctactcc tagaaaaaag ataccatcaa tatttccagg gtatggaatg aaggaaaata
      301 agagccagaa cttggaagta ctagggaact gcacctcagg atacactgac tccaaggttt
      361 tacctgtaat gaacagttta ctgtcaatac atagttaatt acagtcaatt cttgggtttc
      421 tttaggtctg actgatctga ccctggatgg cagccttcag tatgggtccg gtgaagggca
      481 cggtgtgcct ccggccattg gtggtgaagg tcagggtgga gcggttgctt ccgatgaagt
      541 actctgtttt ttgtttattc tctctctggg aggccacacc atccttgtag gcctcgatgg
      601 cgatgatctt tcttaggtcg atgttgatgg tccgcttgga gcctgtgaag atcagtcgct
      661 tgttggttaa gaccagggtg ccctgatcta tcttccggag ttcctcatgg gattcactgc
      721 gggctgctac actccccacc cggaaggaaa cccccttcgt cacccggatg gttggaccag
      781 catatccccc gtaggttttc cggactgccc ggggctcgtg gaagctgatg ttacctaaaa
      841 ctagatgtgc tacttctttc ttcttcagga tgatgggagg attggacaga ggagttaaat
      901 taacagttcc ctgggccaac tggttgacga aggttaccaa gtcattctgg gcagtctgtt
      961 cttctattag ttgctgtttt ttcctttcct tttcttctat gattcgctgc tcctcattgg
     1021 agattccgcc cttagctatg cgggtccact cctcctctgt taagatcttt ccacggtacc
     1081 tttgccagaa ctggcaacct ttatcgtaga tatcctttaa cttgtatttt gatccctttt
     1141 tttcgaatac agccccacag tagtcgcact ctaagagttc tttagtgctg agcatg
//
LOCUS       DAOQVW010000555         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_171287_length_1191_cov_2.934859, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000555 DAOQVW010000000
VERSION     DAOQVW010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171287_length_1191_cov_2.934859"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>1191
                     /locus_tag="PKK85_09500"
     CDS             25..>1191
                     /locus_tag="PKK85_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNS26288.1"
                     /translation="MQTREVTLSGHIIDSLTLPTALDLIMDLGGDFKILEFEVGKRKK
                     DISHARIRVAADSESHLGEILDELTEIGAMVVELEEVNLEPSTKDRTLPQDFYSTTNH
                     PTFIRYQGEWIPVDEIEMDCMIVVDPQNRRALCKPIGRVFKGDLVVVGREGIKVEPPE
                     RPRGKKGVFEFMSSEASSEKPLKSIIKNIATEIKELKSRGGKIAVVGGPAIVHTGSGP
                     ILARMIREGLVDVIFAGNALATHDIESALYGTSLGMCIKSGESVIRGHRHHIYAINEI
                     NKAGSIKDAVKQGVLAKGIMYECVKNEVPFVLAGSIRDDGPLPDVITDVLLAQDEMRK
                     YVRDVDMVIMISTMLHSIATGNILPSHVKSICVDINPATVTKLADRGSAQVVGIV"
BASE COUNT          362 a          270 c          296 g          263 t
ORIGIN      
        1 aaaacacacc atgaggaaga taacatgcaa acaagagaag ttaccctttc tggccatatc
       61 atagattctt taacccttcc caccgcactg gaccttataa tggatctggg aggagacttc
      121 aagatattgg aatttgaggt aggtaaaaga aagaaagaca tcagccatgc caggatacgt
      181 gtagcagccg atagtgaatc acacttagga gaaatactgg atgaactcac agaaatcgga
      241 gccatggttg tggagttaga ggaagtgaac ttggaacctt ccaccaagga ccgcacccta
      301 ccccaagatt tctattccac caccaaccat cccaccttca tccgttacca aggggaatgg
      361 ataccggtag atgaaattga aatggattgt atgatagtgg ttgaccctca aaacagaagg
      421 gccctatgca aacccatagg acgtgtattt aaaggtgact tagtagtggt gggccgggag
      481 ggaattaaag ttgaaccacc agaacgaccc aggggtaaaa aaggcgtttt cgaatttatg
      541 tccagtgaag cctcctctga aaaaccatta aaatccatca ttaagaacat agccacagag
      601 atcaaggaac ttaaaagtag aggagggaag atagccgtgg tgggtggacc ggccatcgtg
      661 cacactggat caggccccat actagccagg atgatccggg aaggactggt ggatgtgata
      721 tttgcaggca acgccctggc cacccacgac attgaaagcg ctctttacgg tacttccctg
      781 ggaatgtgca ttaaaagcgg agagtctgtt attcgtggac atagacacca tatttatgcc
      841 atcaacgaga taaacaaggc aggttctatt aaagacgccg tgaaacaggg cgtacttgca
      901 aaagggatca tgtacgaatg tgttaaaaat gaggtgccct tcgtactggc cggttccata
      961 cgggatgatg gccccctacc agatgtcatc accgatgtac tcctggccca ggatgaaatg
     1021 cgcaagtatg ttcgggatgt ggatatggtg atcatgatct ccaccatgct tcactccata
     1081 gccaccggaa acattcttcc ctcccatgtg aagagtatct gtgtggacat taacccagcc
     1141 accgtaacca aactggcaga tcggggcagt gcccaggtag taggtattgt c
//
LOCUS       DAOQVW010000556         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_172015_length_1188_cov_3.327449, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000556 DAOQVW010000000
VERSION     DAOQVW010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172015_length_1188_cov_3.327449"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKK85_09505"
     CDS             <1..199
                     /locus_tag="PKK85_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016358485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNS26289.1"
                     /translation="DKPVVAYIAGVTAPPGKRMGHAGAIIEGDSGTAQSKKTALENAG
                     VKVADRPSQISELIKKLYKSS"
     gene            217..>1188
                     /gene="hmgA"
                     /locus_tag="PKK85_09510"
     CDS             217..>1188
                     /gene="hmgA"
                     /locus_tag="PKK85_09510"
                     /EC_number="1.1.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase (NADPH)"
                     /protein_id="HNS26290.1"
                     /translation="MPEKDQMVKKLLKGEIKLHQVENYTDGVEEATQIRHRFAQKISC
                     AELEHLAQYSMDMGEALKRNIENPVGAIQVPVGIAGPLLVKGEHAQGEFYVPLATSEG
                     ALVASVNRGCSVIKTSGGAKVGIVADKMTRAPVIKAPNLREALKIRTWIEDNFSALKE
                     AAENTTRHGKLVKIDPIVVVGRYLYPRFSYTTGDSMGMNMVTMATEAALEVLSQNTGA
                     HVVALSGNLCVDKKPSALNLTEGRGKSLVAEVLVPREVVEKKLHTTVEAIVEVNIAKN
                     LVGSAISGSMGFNAHYANMIGAIFLATGQDEAHIVEGSLGITVAQEDE"
BASE COUNT          328 a          281 c          338 g          241 t
ORIGIN      
        1 cgacaaacca gtggtggcct acatcgcagg ggttactgct cccccaggta agagaatggg
       61 gcacgccggg gccatcatcg agggtgacag tggaactgct cagagtaaaa aaaccgccct
      121 ggagaatgca ggtgtgaaag tggctgaccg gccctcccag atatcggagc tgattaagaa
      181 actttataaa tcatcttaat attaaaagtg agtcttatgc ccgaaaaaga tcagatggtg
      241 aaaaaactcc taaaaggtga gataaaactc caccaagtgg aaaactacac tgatggtgta
      301 gaggaagcaa cccagatacg tcatcgcttt gcccagaaga tatcctgtgc agagttggag
      361 cacctagccc agtactccat ggacatgggg gaagccctga agcgtaacat agaaaatcca
      421 gtgggagcca tccaggtacc ggtggggatc gctggtcccc tccttgttaa gggagaacat
      481 gcccagggag aattttacgt acccctggct acctcggaag gtgccctggt ggcatcggta
      541 aaccgtggtt gttcagttat aaaaacctct ggtggggcta aggtgggtat cgtggctgat
      601 aaaatgactc gagctccagt tattaaggca cctaacctta gggaagccct taagatccgg
      661 acctggatcg aggacaactt cagtgcattg aaagaagcag ccgagaacac cacccgccac
      721 gggaaactgg tgaaaattga ccccatagtg gtggtgggcc gttacctgta cccccgcttc
      781 tcctacacca ccggggacag tatgggtatg aacatggtaa ccatggccac tgaagctgca
      841 ctggaggttt taagccaaaa tacaggagcc catgtggtgg ccctctcagg taacctctgc
      901 gtggataaaa aaccatctgc cttgaacctc actgagggaa ggggtaagag cctggtggct
      961 gaggtcctgg ttcccaggga ggtggtggag aagaaactgc acaccacagt ggaggccata
     1021 gtggaggtta acatcgccaa aaatcttgtt ggatctgcta tatcaggtag tatgggcttt
     1081 aacgcccact atgccaatat gatcggggcc atattcctgg ccaccggcca ggacgaggcc
     1141 cacatagtgg agggtagtct ggggataacc gtggcccagg aagatgag
//
LOCUS       DAOQVW010000557         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_172822_length_1185_cov_2.407965, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000557 DAOQVW010000000
VERSION     DAOQVW010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172822_length_1185_cov_2.407965"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PKK85_09515"
     CDS             complement(<1..588)
                     /locus_tag="PKK85_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNS26291.1"
                     /translation="MLETEICKIKMKNPTMLAAGIMGSTAASLNWAARSGAGAVVTKS
                     FSMEPNPGYANPTTVEVTGGFINAIGLSNPGVELFKTELEKLEGLVPAVASIYGANPQ
                     EFQNVASQIEGMVDMIELNVSCPHASGGCGASIGQDPDATGQIVKAVKQAVKTPVAVK
                     LTPNVTDIVEIAEATQKAGCDAITLINSVGPGMRID"
     gene            790..>1185
                     /locus_tag="PKK85_09520"
     CDS             790..>1185
                     /locus_tag="PKK85_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNS26292.1"
                     /translation="MLKRLVKNHDEVVVETEISPSKYESLKDSSKFKFQTPNKAGEYV
                     RTYLADILIQTDFIETEEELKELIHDTSLNITRDRLQEASEAEDLVLIQAINALDELD
                     ETTSKLAERLREWYSIHLPELDKIKSHEYY"
BASE COUNT          319 a          265 c          271 g          330 t
ORIGIN      
        1 gtctatacgc atgcccgggc ccaccgagtt aataagggtg atggcatcac agccggcttt
       61 ctgggtggct tctgctattt caactatatc agttacgttt ggggttagtt taactgcaac
      121 tggggttttg accgcctgtt taactgcttt aactatctgc cccgttgcgt cggggtcctg
      181 gcctattgat gcgccgcatc cgcctgatgc atgggggcat gatacgttga gctctatcat
      241 atccaccatt ccctctatct gggatgcgac gttctggaat tcctgtggat tcgctccata
      301 gatagacgcc actgcgggta ccaggccttc tagcttctct aactcggttt taaatagttc
      361 tactcctggg ttggagagtc ctatggcatt tataaatcca ccggtgactt ccacggtggt
      421 tggattggca tatcctggat taggttccat gctgaaggat ttagttacca ctgccccggc
      481 accgctcctg gcggcccagt taagggatgc agcggtgctg cccatgatcc ctgccgccag
      541 catggtgggg tttttcatct taattttgca tatctcagtt tcaagcatat ggaccagacc
      601 cagaaaatat taaattgata tcagtataga cttattggta tgaagtgtta tattacctgg
      661 tgtttcacgg gttttattgc ccttgatgaa aattgtgccc tcttagatta tgaacttttc
      721 ccaaagagca agataaccct aaggcttatc gaactactgg aaggtaattt ggtaagagag
      781 gaaaaatctt tgctcaagag actggttaaa aaccatgatg aagttgttgt agagacggaa
      841 atttcccctt ccaaatatga aagccttaaa gattcctcta aatttaaatt ccaaacaccg
      901 aacaaagccg gggaatatgt tagaacctac ctggctgata ttttaattca aactgatttt
      961 atcgaaacag aggaagaatt aaaggaatta atacatgata cttccctgaa catcaccagg
     1021 gaccgcctgc aggaggcaag tgaagcagaa gacctggttt tgatacaggc cataaacgcc
     1081 ctggatgaac tggacgagac caccagtaag ttagcagaac gactgagaga atggtattcc
     1141 atccatctcc cagaactgga taaaataaaa agccacgaat actac
//
LOCUS       DAOQVW010000558         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_173320_length_1183_cov_2.269504, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000558 DAOQVW010000000
VERSION     DAOQVW010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173320_length_1183_cov_2.269504"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..>1183
                     /locus_tag="PKK85_09525"
     CDS             64..>1183
                     /locus_tag="PKK85_09525"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS26293.1"
                     /translation="MESIMNALSGGYIIPGLAADPSYADVLPTGTSMYSLDTTKMPTE
                     AAFNAAKRIVDKQLVEYYQKYGKFPELVTIIMWGTELLRTEGIGIAQFLYYMGVTPTW
                     SETGDVNGIKLIKLSELTIKLDNGKVIKRPRIDVMTTAVTSNVNWLSLMVDAVKLVNS
                     VNESTSVNYIKKHYAQHKSLDRIFGLPGAILEGTGVSDFLPNTSKWENSPDMIKDAAE
                     IYLSRISNAWTVDENGRIVVSSNRNTFEYLLKNTDLITQNIDSTWRFLDSSDYYDWFG
                     GALLAAQYLGNNPDTSVVDIRDKNNIITRDLAGELEFETRGMLLNPKYQDGLLNSGPS
                     GWLEYAARIENLFAFNAINKNIYGGNLVSDSMWDQIAQT"
BASE COUNT          381 a          227 c          264 g          311 t
ORIGIN      
        1 aagctttatg ccaacctcaa attctgcgtg gaaaccattt caaagttaaa atcgaacata
       61 gaaatggaat ccataatgaa cgctctaagt gggggctata taatacctgg tctggcggca
      121 gatccatcat atgctgatgt tttacctacc gggaccagta tgtattccct ggacaccact
      181 aaaatgccga ctgaagcagc attcaatgcg gctaaaagga tcgttgataa acagttagtt
      241 gaatattacc agaaatatgg taaattccca gagttagtta ccataatcat gtggggtacc
      301 gagttactac gtaccgaagg tatcggtatt gcacagttcc tatactacat gggtgtcacc
      361 ccaacatgga gtgagacagg agatgtaaac ggaattaagt taattaagct gtccgagtta
      421 accatcaaac tggataatgg caaagttatc aagagaccac gtatagatgt catgacaacc
      481 gcagtaacca gtaatgtgaa ttggttaagc ttaatggtag atgcagttaa gttggttaac
      541 tctgtaaatg aaagtaccag tgttaactac atcaaaaagc attatgccca acataaatct
      601 ttggatcgta tctttggttt gcctggggct attctggaag gaacgggagt atccgatttc
      661 ctgccaaaca cttctaagtg ggaaaatagt ccagacatga tcaaggatgc agcagaaata
      721 tacctctcaa ggatatcaaa tgcatggacc gttgatgaaa atggccgtat cgttgtatcc
      781 agcaatcgaa atacctttga atatctattg aagaatactg atttaattac tcaaaatata
      841 gacagtacat ggagattcct ggattcaagt gactactacg attggtttgg tggagcgctt
      901 ttagccgccc agtatctggg taacaatccc gacacatctg ttgtggatat acgggacaaa
      961 aacaacatca tcaccagaga tttagctgga gaattggaat tcgaaactag ggggatgttg
     1021 ttgaacccaa agtatcagga tggtctctta aacagcggcc ccagtggttg gctggaatat
     1081 gccgcccgga tagaaaactt atttgctttt aacgcgatca acaaaaacat ctacggaggg
     1141 aatctggtca gtgattccat gtgggatcag atagctcaaa cac
//
LOCUS       DAOQVW010000559         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_174008_length_1180_cov_2.691556, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000559 DAOQVW010000000
VERSION     DAOQVW010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174008_length_1180_cov_2.691556"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKK85_09530"
     CDS             complement(<1..283)
                     /locus_tag="PKK85_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNS26294.1"
                     /translation="MAIVVAKFGGTSIGNGDRIKKAAQSVVKEYMKGRKMVVVVSAIN
                     KTTDDILQVVEGAIGESITEKQMADVVSMGEITSVRVFASTIESLGVKSE"
     gene            complement(297..512)
                     /locus_tag="PKK85_09535"
     CDS             complement(297..512)
                     /locus_tag="PKK85_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HNS26295.1"
                     /translation="MGNIRTTFVKRTAKELIETYPGKFTTDFEENKKMVENFSTVSTK
                     HLRNKIAGYITRLVKQGYVSPLIRSEA"
     gene            complement(516..809)
                     /locus_tag="PKK85_09540"
     CDS             complement(516..809)
                     /locus_tag="PKK85_09540"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNS26296.1"
                     /translation="MDRVEALRRLEDSRRQIDILDHEIMELIAQRTFLAGEIVQAKIV
                     LGMDIEDKKREAHIQEKIKEIALEKKIDPTSLSQIMKILTSLSKKEQKKLLKR"
     gene            complement(811..>1180)
                     /locus_tag="PKK85_09545"
     CDS             complement(811..>1180)
                     /locus_tag="PKK85_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNS26297.1"
                     /translation="MEGQNILIYMPHRKSPTAQSNVARMKLLAPWVKMAFQEALQGDF
                     YKALTLNGLLYCAALRFEPIIALEALEAGAYAAGLSGTGPSFVALARGEGADQVEEVW
                     KSYPGRVIRTDVDNQGTRVI"
BASE COUNT          275 a          293 c          220 g          392 t
ORIGIN      
        1 tctctgactt gacacccaat gattctatgg tggatgcgaa aacacgtaca ctggtgattt
       61 cacccattga aaccacgtca gccatttgtt tttcggttat agattctcct atagctccct
      121 ccaccacttg caggatgtca tcggtggttt tatttatagc cgaaactacc acaaccattt
      181 ttcggccctt catgtactcc tttaccactg actgggcagc tttcttgatc cgatctccat
      241 tcccaatgga tgttccaccg aatttggcca caacgattgc cattccccgc ccccagctag
      301 gcttctgatc taatcagcgg gctaacatat ccttgtttta ccaaacgagt aatgtaacct
      361 gctattttat ttctaaggtg tttggtgctt actgtggaaa aattttccac cattttcttg
      421 ttttcctcga agtcagtggt gaatttaccg ggataagttt caattaattc tttagcagtt
      481 cttttaacaa atgtagttct aatattgccc atgttctacc tctttaatag ttttttctgt
      541 tctttcttac tgagactcgt gagaatcttc atgatctggc taagactagt tgggtctatt
      601 tttttctcca gagcaatttc ttttattttt tcttgaatgt gtgcttctct ttttttatct
      661 tcaatatcca tacccaagac tattttggct tggacaatct ctccagctag aaaggtgcgc
      721 tgggcgatga gttccatgat ttcatggtcc aaaatgtcaa tttgacgccg agaatcttcc
      781 aaccgtctta gagcctctac cctatccatc tcaaatcacc ctggttccct ggttatccac
      841 atcagtacgg ataaccctac cgggatatga tttccaaacc tcttccacct gatcagcacc
      901 ttcgcctctt gctaaagcaa cgaaggacgg gccagttccg gataatcctg ctgcgtaagc
      961 acctgcttcc agggcttcca gtgcgattat aggttcaaat ctcagggcag cacaataaag
     1021 caatccgttt aaggttaatg ccttgtaaaa gtcgccttga agggcttcct gaaaggccat
     1081 tttcacccag ggggctagga gtttcatcct ggccacatta gattgggcag tcggtgactt
     1141 tctatggggc atgtatatta atatattttg cccctccatg
//
LOCUS       DAOQVW010000560         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_175540_length_1174_cov_2.108132, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000560 DAOQVW010000000
VERSION     DAOQVW010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175540_length_1174_cov_2.108132"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..494
                     /locus_tag="PKK85_09550"
     CDS             258..494
                     /locus_tag="PKK85_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26298.1"
                     /translation="MVKKIKENNSNEQGHDELKTAKKSVKMEICYLCHKEFDMNQDDA
                     SRYRYGKFPLCDYCAEFYGFYFDDPDKRRREKFD"
     gene            complement(499..>1174)
                     /locus_tag="PKK85_09555"
     CDS             complement(499..>1174)
                     /locus_tag="PKK85_09555"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030022.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNS26299.1"
                     /translation="TVKATGADLGIAHDGDADRTIWIDENGEFVMGDKTFALVEKYML
                     KENPGGIIVTTVATSTAIYDIAEEYHGEVIATAVGDLLVARELKNRDGLFGGEENGGL
                     IFPDFVYGRDAALSTAKILEIMARENKPLSKLITELPNYESVKLKVECPDELKTEIME
                     KITQDTRQYQVDTTDGVKIFRDEGWLIIRPSGTEPIFRCFSEAKHKEDAQMMAEWGIS
                     LVKKYL"
BASE COUNT          330 a          255 c          214 g          375 t
ORIGIN      
        1 tccctcattt gctgcattat gtctactgtt tccagtattt tctccccact ccctgtaata
       61 ccccaagcaa tctttctttt tttgttcatg aatctattat atgattaatt cataatttaa
      121 tttttgctta attaggagtt ctaaggtaaa ccctcctcca cccatttcat actctcccaa
      181 tgttatatag aatggcgaat taaaaagaag tagtgcaatc atattgatga tatagtggta
      241 gtgatcgaaa agttgggatg gtgaagaaaa taaaagagaa taatagtaat gaacaaggcc
      301 atgatgagtt aaaaacagcg aagaagtctg tgaagatgga aatctgctat ttatgccata
      361 aagagtttga catgaaccag gatgatgcca gtcgctatcg ttatggtaag ttccccctgt
      421 gtgattactg tgccgagttt tacggctttt actttgatga ccctgataaa agaagaaggg
      481 aaaaatttga ttaagcgatt ataaatattt tttaaccaga gatatccccc attcagccat
      541 catttgtgca tcttctttgt gtttagcctc tgaaaaacat ctgaatatgg gttctgtccc
      601 ggaagggcgt ataataagcc atccttcgtc ccggaatatt ttaaccccat cggtggtatc
      661 cacctgatac tgacgggtgt cctgggtaat tttctccatg atctcagttt taagttcatc
      721 tggacattct accttcagtt taactgattc atagttaggc aattcggtta taagttttga
      781 caatggtttg ttttcccggg ccattatttc caaaattttg gcggtggata atgctgcatc
      841 ccgtccgtat acaaagtcgg gaaatataag cccaccattt tcctctccac caaacaatcc
      901 atctctattt ttaagctccc gggccactaa aagatcaccc actgcggtgg ctataacctc
      961 accatgatat tcttcagcga tatcgtagat ggcagtggaa gtagccactg tagtgacgat
     1021 tattcccccc ggattctcct ttaacatgta tttctccacc agggcaaaag ttttatcacc
     1081 catgacaaat tctccatttt catcaatcca gatggtacga tcagcatcac catcatgggc
     1141 tattccaaga tcagccccag tagctttcac cgtt
//
LOCUS       DAOQVW010000561         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_175649_length_1173_cov_3.827370, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000561 DAOQVW010000000
VERSION     DAOQVW010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175649_length_1173_cov_3.827370"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKK85_09560"
     CDS             <1..341
                     /locus_tag="PKK85_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNS26300.1"
                     /translation="GSSRPASGAEHKFSHALDLIAPQPALHGEQCAVGTIMMMYLHGG
                     DWELVRDTLKAIKAPTTARELDIKPEYIIEALSTAHTIRRERYTILGDRGLTYQAAEK
                     LALHTGVIES"
     gene            546..977
                     /locus_tag="PKK85_09565"
     CDS             546..977
                     /locus_tag="PKK85_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HNS26301.1"
                     /translation="MITLIGKKLADEGLKFVFYGASSQCMNCRFKATCIDSLEPGRIY
                     RIKTVKNTTHPCPVHMGGEVKVVEVDRALIKIHIDSKKAFEGSSYSYKPRECQEECSM
                     RELCNPEGLYLGDKCRIIKKLGKPSQKCPRGYDLTTVLVKY"
     gene            995..>1173
                     /locus_tag="PKK85_09570"
     CDS             995..>1173
                     /locus_tag="PKK85_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HNS26302.1"
                     /translation="MDPKKRVGLEAALRVEDGMVVGLGTGSTTHHFIKSLAERLKEEE
                     LEVWGIPTSYQSYSLA"
BASE COUNT          325 a          272 c          317 g          259 t
ORIGIN      
        1 ctggtagcag caggccagcc agtggtgctg aacataaatt cagccacgcc ctggatctga
       61 tcgcacccca accagccctg catggtgaac agtgtgccgt gggcaccatc atgatgatgt
      121 acctccatgg cggggactgg gaattagtac gggacaccct gaaggctatt aaggcaccta
      181 ccactgcccg ggaattggat atcaaacctg aatacatcat tgaagctctg agcacggctc
      241 acactatacg ccgggagcgc tacaccatac tgggtgatcg gggactcacc taccaggccg
      301 cggagaaact ggccctgcat accggggtaa tcgaatcctg aacctttcag taggtaggag
      361 caggatccag cattgaagag cccagttcct tttacttata ccctgtctct agatgtaccc
      421 cagcagggaa atagtatata aagtgggttc atgataaaaa gtttagagta gttagaaaac
      481 cagttctggt aataattttt aaagatgcat agcacaaaat cgtaagaccc cagggggggt
      541 acagcgtgat aactcttatt ggaaaaaaat tggcagatga gggactcaaa ttcgtttttt
      601 acggggcatc cagccagtgc atgaattgtc gctttaaagc cacctgcata gactccctgg
      661 agcctggacg tatctaccgt atcaagactg tgaagaacac cacccatccc tgcccagtgc
      721 atatgggggg agaggttaag gtggtggagg tggaccgggc cctaatcaag atccatatag
      781 attccaagaa agccttcgaa gggtcgagtt acagctacaa acccagggag tgtcaggagg
      841 aatgttccat gcgagagttg tgcaacccgg aaggacttta cctgggtgat aagtgcagga
      901 tcatcaagaa attaggtaaa ccatcccaga agtgtccccg tggttacgat ctgactacgg
      961 tgctggttaa atactaagtg catggtgcat tattatggat cctaaaaaac gtgtaggact
     1021 ggaagctgcc ctgagggtgg aggatggaat ggtggtggga ctgggaactg gctccaccac
     1081 ccaccatttc attaaaagcc tggctgagag gttaaaggag gaggaactgg aagtgtgggg
     1141 tatacccacc tcttaccagt cctactccct ggc
//
LOCUS       DAOQVW010000562         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_175821_length_1173_cov_1.765653, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000562 DAOQVW010000000
VERSION     DAOQVW010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175821_length_1173_cov_1.765653"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PKK85_09575"
     CDS             <1..860
                     /locus_tag="PKK85_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNS26303.1"
                     /translation="PIVMGNANRALSAPLSIWNDQQDSISERDTGWMQFFAENAQEAM
                     DFVIQAYKISEDHEVLLPSMVCVDGFILTHTVEPVDIPSQEDVDKFLPPYEPISYLDP
                     KDPMSLGTFTDPDYYMEARHDMEVAMEGAKDIIRRVNKEFEEVFGRKYNLVEKYKTED
                     AEIILVAMGSICGTIKTVIDRLREKGEKVGLLKVISYRPFPQEDIYEAVKGARRIAVL
                     DKNISFGIGGVLFNEIKAKMDVDASGFILGLGGREVSPDDIIGIVEKTRNPTTETNQI
                     NWIGLKEEE"
     gene            863..>1173
                     /locus_tag="PKK85_09580"
     CDS             863..>1173
                     /locus_tag="PKK85_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNS26304.1"
                     /translation="MDIPEKEFLAPGHRACAGCGATIGVRLALKMLGENTVAVSATGC
                     LEVITTPYPETSWKIPWIHVAFENAGAVASGVEKALKAQGKDDVTVVAFAGDGGTADI
                     G"
BASE COUNT          375 a          262 c          286 g          250 t
ORIGIN      
        1 cacccatagt aatgggaaat gcaaacagag cactatctgc acctttaagt atatggaacg
       61 accaacagga ctccatctct gaaagagata ctgggtggat gcagtttttc gctgaaaatg
      121 cccaggaagc catggacttt gtaattcaag cttataaaat atctgaagac catgaagtac
      181 tacttcccag tatggtctgt gtagacggtt tcatattaac ccatacggtt gaaccggtag
      241 acatcccctc acaggaagat gtggataaat ttttaccccc atacgaaccg atatcctacc
      301 tggatcccaa ggatcccatg tccctgggaa ccttcaccga cccggactac tacatggaag
      361 ccagacacga catggaagtg gccatggaag gagccaaaga cattattaga agagttaata
      421 aagaatttga agaagttttc ggacgaaaat acaacctggt tgaaaagtac aagaccgaag
      481 acgctgaaat catcctggta gccatgggat ccatctgcgg aaccataaaa accgtgatag
      541 accgcctcag ggaaaaggga gaaaaagtag gtctcctaaa ggttatctcc taccggccct
      601 tcccccagga ggatatatac gaagcagtta aaggagcccg tagaatagcc gttttagata
      661 aaaacatctc cttcggaatt ggaggagtcc tgttcaacga gattaaagcc aaaatggatg
      721 tagatgcatc tggattcatc ctaggactgg gaggacgtga agtctccccc gacgatataa
      781 tcggcatagt cgagaaaacc agaaatccaa cgaccgagac taaccagatc aactggatcg
      841 gattaaagga ggaggaataa acatggatat accagaaaaa gaatttctag caccaggaca
      901 cagagcatgc gctggatgcg gtgccaccat aggggttaga ctggccctga agatgctggg
      961 agaaaacaca gtagccgtat cggccaccgg ttgcctggaa gttataacca ccccctatcc
     1021 agaaacttcc tggaagattc cctggataca cgttgccttt gaaaatgccg gtgcagtagc
     1081 cagtggagtt gaaaaggctc ttaaagcaca gggaaaagat gatgttactg tagtggcctt
     1141 tgccggagac ggtggaaccg cggatatcgg ttt
//
LOCUS       DAOQVW010000563         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_175897_length_1172_cov_3.319606, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000563 DAOQVW010000000
VERSION     DAOQVW010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175897_length_1172_cov_3.319606"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKK85_09585"
     CDS             complement(<1..677)
                     /locus_tag="PKK85_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNS26305.1"
                     /translation="MINTIYRLVSPKQFEVTYQEVDLNERQVMVRPTHLSICKADQRY
                     YLGDRDPEILNEKLPMALIHEGIGEVVYDPTGNFQVGDLVVLIPNIPQEEDDFISENY
                     LDSSQFRSSGFDGYLQEYVATSPDRIVKLPSSLNPEVAAFTELISVCLHSICRLEKVS
                     HPHGKVVGVWGDGNVGFITSLLLKTIYPHKKVIVFGKNLERMSYFTFADQVYTINQIP
                     PGLTIDEA"
     gene            complement(677..>1172)
                     /locus_tag="PKK85_09590"
     CDS             complement(677..>1172)
                     /locus_tag="PKK85_09590"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HNS26306.1"
                     /translation="NNHNMVIEGGNTRNETIMNAIYYIEDNYDIDAEAVLVTHDSVRP
                     FVTHRIIKENIECALKYQACDTVIPATDTIVSSQDGVTIQDIPNRKVMYQGQTPQSFR
                     ILKLKELYHKLTDEERMVLTDAAKIFTLNQEKVYLVDGDVTNIKITYPYDLKVANTIL
                     QGNR"
BASE COUNT          303 a          247 c          268 g          354 t
ORIGIN      
        1 gcttcatcaa tggtaagacc cgggggaatt tggtttatgg tgtagacttg gtcggcaaat
       61 gtgaaataac tcatcctttc caaattttta ccgaagacaa tgaccttttt atggggataa
      121 atggttttaa ggagtaaaga agttatgaac ccaacattcc catcgcccca cactccaact
      181 accttcccat gagggtggga aactttttcc aaacgacata tggaatggag gcatacactg
      241 atgagttcgg tgaaggcagc gacttcgggg ttcagggagc tggggagttt aactattcgg
      301 tccggggaag tggccacgta ttcctgcaag tacccatcaa aaccactgga tcggaactgg
      361 ctactgtcca agtagttttc ggatatgaaa tcatcctctt cctgggggat gttgggaata
      421 agcaccacca gatctcccac ctggaagtta cctgtggggt catagaccac ttcaccgatc
      481 ccctcatgga tgagagccat gggtaacttt tcattcagta tttctgggtc ccggtcccca
      541 aggtagtacc tctgatcggc cttgcagatg gaaagatggg tgggcctaac cataacttgc
      601 ctttcattta gatctacttc ctgataggtg acctcaaatt gtttgggaga aaccaggcgg
      661 taaattgtat ttatcattat ctattccctt gcaaaatagt attagccact ttaagatcat
      721 agggataagt aattttgatg ttggtaacgt ctccatcaac taaataaact ttttcctggt
      781 ttaaggtaaa tatcttagct gcatccgtca acaccattct ttcttcatca gtgagtttat
      841 ggtacagttc tttaagtttc aatatccgga aggattgggg tgtttgaccc tgatacataa
      901 ccttccggtt ggggatgtcc tggatggtga caccatcttg actgctgact atggtatcgg
      961 tggcaggaat tacggtgtcg caagcttggt atttcagggc acattcaatg ttttctttga
     1021 ttatgcggtg ggtcacgaag ggccgcactg aatcatgggt aaccagaact gcttcagcat
     1081 ctatatcata attgtcctcg atatagtaaa tagcattcat aatagtctca tttctggtat
     1141 ttcctccctc aataaccata ttgtggttat tg
//
LOCUS       DAOQVW010000564         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_176117_length_1172_cov_1.157565, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000564 DAOQVW010000000
VERSION     DAOQVW010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176117_length_1172_cov_1.157565"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PKK85_09595"
     CDS             <1..821
                     /locus_tag="PKK85_09595"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNS26307.1"
                     /translation="DWINRAVRMVARLEEPPEKNYPRQHYLEDQEEFGDLALARVFGP
                     APDEYATSVRTLIENGNWEDEEELVENYTDSMCYAYQKDGTRRDVKAFNHALKGVDMV
                     TQERDNSEYEVTDLDHYYEFLGGLSRTIESQKGEQAEIMVVDSTEEELQVEDLAGVIG
                     RATRTRLLNPDWIEGMLKHEFLGAHKIQNSLEHLLGFAATTSHVENWLFDEAADKLLL
                     DEDMREKIRENNPYAAVRMGEILLETEKRGYWQADEEKIEKLRDLTVNLESYLE"
     gene            complement(837..>1172)
                     /locus_tag="PKK85_09600"
     CDS             complement(837..>1172)
                     /locus_tag="PKK85_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26308.1"
                     /translation="ERFGVDEEHYWSAVKGMGAHEKKEFLQNLRRISENPSVVAMLAS
                     ILHIMDEISWGLIPEDAGKRTAIYMMMALPDILGIEKSNGIREIFNKKDSILENWIRI
                     SSWCIKHLK"
BASE COUNT          353 a          249 c          292 g          278 t
ORIGIN      
        1 tagactggat caacagggca gtgcgcatgg ttgctcgcct ggaggagcct ccggagaaaa
       61 attatccccg acagcattac ctggaggacc aggaagaatt cggagatctg gccctggccc
      121 gggtgttcgg ccccgctcca gacgagtacg ccaccagtgt aaggacactg atagagaatg
      181 gtaactggga agatgaagaa gaactggtgg aaaattacac ggacagcatg tgctacgcgt
      241 accagaaaga tggcacccga agagatgtga aggcatttaa tcatgcactt aaaggggtgg
      301 acatggtcac ccaggagagg gataacagtg aatacgaagt tacagacctg gatcattact
      361 atgaattttt aggcggccta tcccgtacca tagaaagtca gaagggtgag caggcagaaa
      421 tcatggtggt agacagcact gaagaagagt tacaagttga ggacctggca ggagtcatag
      481 gcagggccac cagaacccgg cttttgaatc cggactggat agaggggatg ttgaaacacg
      541 agttcctcgg ggctcataag atccagaaca gtttagaaca ccttttagga tttgctgcaa
      601 ccaccagtca tgtggagaac tggttgttcg atgaagcagc tgacaaatta ctcctggacg
      661 aggatatgcg ggaaaaaatc agggagaaca acccctatgc tgcagtcagg atgggagaga
      721 tactacttga aacagagaaa cgaggatact ggcaggcaga tgaagaaaag atagaaaaac
      781 taagggattt gaccgttaat ctggagtcgt acctggaatg acaccatctt taagatttat
      841 tttaaatgtt ttatacacca ggaactgatc cttatccagt tttccaggat agaatccttt
      901 ttattaaata tttccctaat accattactt ttttcaatcc ccaggatatc tggcagggcc
      961 atcatcatgt agatggctgt tcttttacct gcgtcttctg ggattaatcc ccaactgatt
     1021 tcatccatga tatgtaaaat ggaagcaagc atagccacca ctgagggatt ttcagaaatt
     1081 ctacggagat tctgtaaaaa ttccttcttt tcatgggccc ccataccttt tacagccgac
     1141 cagtaatgtt cttcatccac tccaaaccgt tc
//
LOCUS       DAOQVW010000565         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_176608_length_1170_cov_1.417040, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000565 DAOQVW010000000
VERSION     DAOQVW010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176608_length_1170_cov_1.417040"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PKK85_09605"
     CDS             complement(<1..746)
                     /locus_tag="PKK85_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS26309.1"
                     /translation="MTNLSKKLEILGESAKFDRCSYINTNVDNFLAKQLPGIYNASTR
                     DGCTVPLFKVLMTNKCSNDCRYCINHNKHSFNRVEFSPRDVTSIFLDYYNNRLVEGLF
                     LSSGIPQDPEYSMENMVEVAHKLRMDHGYQGYIHLKILPGSPYDLIKRAVSLADRVSI
                     NLEAATPEGMENLSSTKNYDTDILRRMKWIKRLLNGNKEFSPSGQTTQFLVGAGDETD
                     QQILEKANWLYEKMDLKRSYFSPFEAVKDTP"
     gene            895..>1170
                     /locus_tag="PKK85_09610"
     CDS             895..>1170
                     /locus_tag="PKK85_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HNS26310.1"
                     /translation="MRLVLAGTGSAVGKTTIATGIMKALSEEYTVQPFKVGPDYIDPT
                     YHTLATGNYSKNLDSFFMTDQQIRQGYEGGMKKSNADFGIIEGVRGLY"
BASE COUNT          335 a          235 c          231 g          369 t
ORIGIN      
        1 ggtgtgtctt taactgcttc aaatgggctg aaataacttc ttttaaggtc catcttttca
       61 tataaccagt ttgccttctc caggatctgt tgatctgtct catcaccagc acccaccaga
      121 aactgggtgg tctgtcccga gggggagaat tctttgttgc cattgaggag ccttttgatc
      181 cacttcatac gccttaaaat gtctgtatcg taatttttgg tgctggaaag attctccatt
      241 ccttcagggg tagctgcttc caggttgatg ctaacccggt ctgctaaact aacagccctt
      301 ttaattaaat cataggggct gcctggtaga atctttaaat ggatataccc ctggtatcca
      361 tggtccattc ttaatttatg ggccacttcc accatgtttt ccatggaata ttctggatct
      421 tgggggatgc cagaacttaa aaataaaccc tcaaccaacc tgttattata ataatcaagg
      481 aaaatggaag taacatctct aggtgaaaat tctacccggt taaaactatg tttattatga
      541 ttaatgcagt atctgcagtc atttgagcat ttattggtca tcaatacttt aaataggggt
      601 actgtgcacc cgtctctggt gctggcattg tatatgcctg gtaattgctt tgccaggaag
      661 ttgtcgacgt tggtgttgat gtagctgcac cggtcgaact tggcactttc acccaggatt
      721 tccaattttt tgctcaaatt agtcatggtc tatcttgact atttttctgt tatttaatat
      781 agttcaatct atgggtagag cagataagat atactacaag gtaaatttta tgaaaatgaa
      841 aattattttt cccagacaaa tcttatttaa ataaaatcaa tctcaattaa tattatgaga
      901 ttagtccttg ccggtaccgg aagcgcagtc ggaaaaacca ccatagccac cggtataatg
      961 aaagcattat cagaagaata tacagttcaa ccctttaaag tggggccaga ctacattgat
     1021 ccaacctatc acactctagc cacaggcaat tactcaaaaa atctggattc ctttttcatg
     1081 accgaccaac agatcagaca gggatatgaa ggaggaatga aaaaatctaa cgctgatttt
     1141 ggcataatcg agggcgtgag gggtctttat
//
LOCUS       DAOQVW010000566         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_176713_length_1169_cov_2.663375, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000566 DAOQVW010000000
VERSION     DAOQVW010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176713_length_1169_cov_2.663375"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PKK85_09615"
     CDS             complement(<1..648)
                     /locus_tag="PKK85_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNS26311.1"
                     /translation="MTVIIFSLDKFQSDWLLYYFTFGLVLGNLLLPTWFFQGMEKMRY
                     ISILNIGISLIYTAAIFIFVRQPADYTYVPLISALGTIIIGVYSLRLVKRQFGVGFTR
                     PSREDIRYQLEEGWYVFISTVAISLYTISGTFILGFFASNTIVGYFSVAYRIISIANG
                     LLSPISQSIYPYISSLAVKSREEAIYFIKKTTTIMGALSLLVSFLIFIFAGLILYI"
     gene            complement(669..992)
                     /locus_tag="PKK85_09620"
     CDS             complement(669..992)
                     /locus_tag="PKK85_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNS26312.1"
                     /translation="MKLPVPSRINEILENKDYQRILENILSLFSLQGLNYILPLVTFP
                     YLTRVLGPEKYGLVAFALAFIGYFQILTDYGFNMSATREISIHREDEERVSRIYSSVL
                     ATKAS"
BASE COUNT          306 a          265 c          309 g          289 t
ORIGIN      
        1 gatatacagg atcaacccgg cgaagatgaa gatcagaaag gataccagga ggctcaatgc
       61 gcccatgatg gtggtggttt ttttaataaa atagatagct tcttctctgg atttaactgc
      121 caggctactt atgtagggat atattgattg ggatatggga cttaaaagtc cattcgcaat
      181 gctgatgatc cggtaagcca cggaaaagta tccaactatg gtgttgctgg cgaaaaagcc
      241 caggatgaag gtgccactga tagtgtagag gctgatggcc acggtggata tgaaaacgta
      301 ccagccctcc tccaactggt acctgatatc ttcccgggaa ggccgtgtga agccaacccc
      361 aaactgtctt ttaaccagcc tcagggaata gactccaatg attatagtgc ccagggcact
      421 gattaagggt acgtaggtgt aatctgctgg ttggcgtacg aaaataaata tggcggcggt
      481 gtagataagg ctgatcccga tgttgaggat gctgatgtag cgcatcttct ccatgccctg
      541 gaagaaccag gtgggcaaaa gcaggttacc caggaccagt ccgaaggtga agtagtaaag
      601 gagccagtca ctctggaact tgtccaaact gaagataatg accgtcatca gcaggaaact
      661 cagcaccatc aagaggcctt ggtggccagt accgaactgt agatccggct aaccctctcc
      721 tcatcctcac ggtggatgga gatctcccgg gtggcggaca tgttaaagcc ataatcagtc
      781 aggatctgga agtagccaat gaaggccagg gcaaaggcca ccaatccata cttctctggc
      841 cccaggaccc tggtaaggta ggggaaggtg acgaggggta atatatagtt caggccttgg
      901 aggctgaaca gggatagtat gttctccagg atcctctggt agtccttatt ttctaggatt
      961 tcattgatcc gggatggtac tggcaacttc atattaataa aaaggtatct aggggattgg
     1021 ccttataatt ttctatagtt actcatttta ctagtcccgg taggcggatc ccctcttctt
     1081 gatattgtcc cggatcaccc ggtgctcctc cctctcctgt ccagtttcca cgcactccct
     1141 gcggagtaac tccatgatct ggttggtgg
//
LOCUS       DAOQVW010000567         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_177647_length_1166_cov_1.426643, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000567 DAOQVW010000000
VERSION     DAOQVW010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177647_length_1166_cov_1.426643"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PKK85_09625"
     CDS             complement(<1..1104)
                     /locus_tag="PKK85_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS26313.1"
                     /translation="MVFTEETLGNFLEAMVEKNPDQEFMVYPDRDLRFTYKEFDERAN
                     QMAKGLLEIGIKKGDHVGIWAKNVPDWLTYMFATAKIGVVLVTVNTAYKSHELAYVLK
                     QSDMKALAIIDGFQDVDYIQTLYELIPELKSHERGKLKSEKFPFLESIIYLGQEKHRG
                     MYNTNEILLLGKHGNEELFQEIKATVRNDEVVNMQYTSGTTGFPKGVMLTHRNILNNG
                     YYIGERQKFTEKDRLCITVPLFHCFGIVLAVMAAYSHGATMVMVEVFDPLMVLAAVQK
                     ERCTALYGVPTMFIAEYSHPMFEMFDLTSLRTGIMAGSTPPIEAMKRVVNDMNMTEIT
                     SVYGLTEGSPGFTQTSVDDPLEKRVETVGKPLPE"
BASE COUNT          259 a          276 c          249 g          382 t
ORIGIN      
        1 ctctggtaat ggttttccca cggtttccac cctcttctcc agggggtcgt cgacgctggt
       61 ctgggtaaat ccgggtgagc cttccgtgag gccgtataca ctggttatct ccgtcatgtt
      121 catgtcgtta accacccttt tcatggcttc gataggaggg gtggaaccgg ccatgatacc
      181 ggtgcgtaaa ctggtaagat cgaacatctc aaacatggga tggctgtact cagctataaa
      241 catggttggc actccgtaga gtgctgtaca gcgttctttt tgaactgctg ccagtaccat
      301 caggggatcg aagacctcca ccataaccat ggtggcccca tggctataag cagccatcac
      361 tgccagtaca ataccaaaac agtgaaataa cggtactgtg atacatagtc tgtctttttc
      421 tgtgaatttc tgtctttccc ctatgtagta tccgttgttc aggatgtttc tatgagttag
      481 cattacgcct ttgggaaatc cggtggttcc agaggtgtac tgcatgttga ccacttcgtc
      541 gtttcgtacc gtcgctttaa tctcctggaa taactcctca tttccatgtt ttccaagtag
      601 caagatttca ttggtgttgt acattcccct gtgtttttcc tggcccaggt agatgatgct
      661 ttccaggaag gggaactttt cactctttaa tttacccctt tcatgggatt ttaattcagg
      721 gataagttcg taaagggttt gaatgtaatc cacatcttga aatccatcta ttattgccag
      781 tgccttcata tctgactgtt tcagtacgta ggcaagttca tggcttttat aggctgtgtt
      841 gactgtgacc agcaccactc ctattttggc ggtggcgaac atataggtga gccagtcggg
      901 tacatttttc gcccatatac ccacatgatc tccctttttt attcctatct ccagaagacc
      961 tttggccatt tgattcgctc tttcatcaaa ttccttgtag gtgaagcgta agtcccggtc
     1021 agggtagacc atgaattcct gatcgggatt tttctccacc atggcctcca ggaagttccc
     1081 cagagtttct tcagtaaaaa ccatttttaa aaccttctat ctctttttac ctgtttttaa
     1141 atctctttta ttaagtgagt aatctg
//
LOCUS       DAOQVW010000568         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_177820_length_1165_cov_2.154054, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000568 DAOQVW010000000
VERSION     DAOQVW010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177820_length_1165_cov_2.154054"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..748
                     /locus_tag="PKK85_09630"
     CDS             173..748
                     /locus_tag="PKK85_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNS26314.1"
                     /translation="MEKTLENLTKAFIGESQARNRYTFYSKQATAEGYPQISEIFLET
                     ADNEREHAKWLFKMIQDLKEGELDEIEVGALSPLTLGTTVDNLRAAIAGEHYENSEMY
                     PEFADVAEEEGLIEVSDRLWSIAHAEEHHEDRYKKLLEQVEAGTLFGKKEEVEWTCIK
                     CGYTYKGTEPPAKCPACDHARIYFKLYDEKY"
     gene            779..1162
                     /locus_tag="PKK85_09635"
     CDS             779..1162
                     /locus_tag="PKK85_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNS26315.1"
                     /translation="MTEKEEIYRCNICGNIVEVFHIGTGKLECCQNPMELLEEREEGM
                     GAEKHIPVLEETDEGVKVKVGSLPHPMEENHCIEWVEVVTGDQVHRKVLKPGDAPEAE
                     FKLKLDDISQIVVREYCSIHGLWRS"
BASE COUNT          416 a          217 c          283 g          249 t
ORIGIN      
        1 ttaaaaaaat ttttttaata tataaaattt ttccaatatt agtttttaag ggaatgaacg
       61 ccttaaataa aatgaaaggg tgaaatattc cttaaatgac caaaggaaat ctttaaaaat
      121 tagatgacta aaattaataa gatagaagcc atataatact aatggtgata aaatggaaaa
      181 aaccctggaa aatttaacaa aggcatttat aggagaaagt caggctagga atagatacac
      241 tttctattca aaacaggcta cggctgaggg atatccccag atctctgaga tctttttaga
      301 aacagcagat aatgaacggg aacatgccaa atggcttttt aaaatgatcc aggaccttaa
      361 agagggggaa ctggatgaga tagaggtggg cgccctttcc cccttaaccc tgggcacaac
      421 cgtcgataac ctgagggcgg ccatcgccgg ggaacactat gaaaacagcg agatgtaccc
      481 agaattcgct gatgtcgctg aagaagaagg tttaatagaa gtgtccgatc gtctctggtc
      541 catagcacat gccgaggaac accatgagga ccgttacaag aaactcctgg aacaggtgga
      601 agcaggaacc ctgttcggga agaaggaaga agtggaatgg acctgtataa aatgtggtta
      661 cacctacaaa ggcacggaac caccagcaaa atgccccgcc tgtgaccatg cccggatata
      721 cttcaagttg tacgatgaaa aatactgaaa aggatcaaaa atattgaaga ggattacaat
      781 gactgaaaaa gaagagattt atcgctgtaa catctgtggc aacatagtgg aagtcttcca
      841 catcggcacc ggaaaactgg aatgctgcca gaaccccatg gaactactgg aagaacggga
      901 agagggtatg ggagctgaaa aacacatacc tgttctggaa gaaaccgatg aaggagttaa
      961 ggtaaaagta gggtccctac cccatcccat ggaggaaaac cactgcatcg agtgggtgga
     1021 agtagtaaca ggcgatcagg tccaccgaaa ggtcttaaaa ccaggagacg ctccagaagc
     1081 tgaattcaag ctgaaactgg atgacatcag tcaaatagtg gtaagggaat actgcagcat
     1141 ccatggtcta tggagatcgt gaggg
//
LOCUS       DAOQVW010000569         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_177892_length_1165_cov_1.627027, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000569 DAOQVW010000000
VERSION     DAOQVW010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177892_length_1165_cov_1.627027"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKK85_09640"
     CDS             <1..380
                     /locus_tag="PKK85_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase partner protein"
                     /protein_id="HNS26316.1"
                     /translation="EIFYEYVQDMRDRMNKGMRISENAIWEAAVESLVMMSRGEKKAQ
                     IENEIVGKAIADFRKRYRQALRKGTLDSAPDLDVLLLAKELGAGVVAADEGIKVWAER
                     LGLRFLSAKSFPKMMNEYLKYYE"
     gene            500..>1165
                     /locus_tag="PKK85_09645"
     CDS             500..>1165
                     /locus_tag="PKK85_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNS26317.1"
                     /translation="MDIQRPRGTRDFLFEEMKERKEIETTLRRVFETYGYGEIKTPLF
                     EDLALFTKKSGEGIVEQIYNFQDKGGRDLALRPELTAPVARLYINELQKSPKPLKMYY
                     FGSCFRYERPQKGRWRQFWQMGCELIGGKSPESEAEIITMAAHSLEELGLTDFEIHIG
                     NLGIIRGILDQADVEKGVQDIIMAIIDKGEIDELLKTLNELKIDPKTSEILLTLVGLV
                     GHRE"
BASE COUNT          378 a          239 c          314 g          234 t
ORIGIN      
        1 cggagatatt ctatgaatac gtacaggaca tgagggaccg gatgaacaag gggatgagga
       61 tatcagaaaa cgccatctgg gaagcagccg tggaatccct ggtcatgatg tcccggggag
      121 agaaaaaagc ccagatagag aacgagatag tgggcaaggc catagccgac ttcaggaaac
      181 gctacaggca ggccctaaga aagggaaccc tggacagcgc tccggacctg gatgtgctcc
      241 tcctggccaa ggaactggga gcaggggtag tcgcggctga tgagggaatt aaagtctggg
      301 cagagaggct gggactcagg ttcctcagtg ccaagtcatt ccccaagatg atgaacgaat
      361 acctaaagta ttatgaatag aacttacatt gaaaccctca ccttataaga tcggtggggg
      421 taaaaattat ttttctaaaa aaaaatttat tctgaaaaat tttatccaaa tataattatt
      481 tctaaatgga gaaccaccaa tggacataca aagaccacga ggcacgagag attttctttt
      541 tgaagaaatg aaggagagaa aagagataga aaccacccta aggagggtgt ttgagactta
      601 tggatatggg gagattaaaa ccccactgtt tgaagatctg gcccttttca ccaagaaatc
      661 aggggaagga attgtggaac agatatacaa cttccaggat aaagggggaa gggacctggc
      721 actccggccg gagctaactg ccccggtagc ccggttatac atcaacgaac tgcaaaaaag
      781 tcccaaacca ctgaagatgt actacttcgg gagttgtttc cgctatgaaa gaccccagaa
      841 gggccggtgg aggcagttct ggcagatggg atgtgaactc atcgggggaa aatcccccga
      901 gtctgaagca gagatcatca ccatggccgc ccactcactg gaagaactgg gacttacaga
      961 cttcgagata cacatcggaa acctggggat aataaggggg atactggacc aggcagatgt
     1021 tgaaaaggga gtccaggaca tcatcatggc cataatagat aaaggtgaaa tcgatgagct
     1081 gcttaagaca ttaaatgagc ttaaaattga tcctaaaacc agtgaaatac tcctcaccct
     1141 ggttggcctg gtgggccacc gggaa
//
LOCUS       DAOQVW010000570         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_178336_length_1163_cov_2.147112, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000570 DAOQVW010000000
VERSION     DAOQVW010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178336_length_1163_cov_2.147112"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKK85_09650"
     CDS             complement(<1..604)
                     /locus_tag="PKK85_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HNS26318.1"
                     /translation="MSHNNKIANRKDIAINKLKSLGERIIEEINQNNVPALKVPSRGT
                     SNIIYDEDKRYYVLGDRYGQRSLGNVKQIPKIAQMVYVANFCKDLVRRGKTATLREMY
                     YVSEGWDVDFGDQQESNIVGEDLEVTLGMTREDLGLMPEEDGASVYGNLIMKEDDVEI
                     NALRLGKSGYTISPTIDDVEFLDHDVDKVIAVETMGMFHRL"
     gene            complement(594..>1163)
                     /locus_tag="PKK85_09655"
     CDS             complement(594..>1163)
                     /locus_tag="PKK85_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HNS26319.1"
                     /translation="TSRKPKTYRGGVAFVIEAGISYGGDSGRLVGEQKKAEIMRFANR
                     VPLTFDQGSCAITEALKSIDWKRYGIRDLDNAPITVFVNIVSTNVPYLSTGKQSVAPE
                     PEILHEVRQATMKIARNLQKYIRAKRAAKEEAMRSKIFENLVPVIIREAAVLAETDVP
                     EYDSVLAKVTRRAKFENIPLESEEGEDES"
BASE COUNT          228 a          304 c          264 g          367 t
ORIGIN      
        1 ccagacggtg gaacataccc atggtttcca cggcgatcac cttgtccacg tcgtggtcca
       61 gaaattccac gtcatctatg gtaggtgata tggtgtaacc agatttacca agtctaaggg
      121 cgtttatctc cacgtcatct tccttcatga tgaggttacc atagactgag gcaccgtctt
      181 cttcgggcat gagtcccagg tcttccctgg tcatacccag ggtaacttcc aggtcctctc
      241 ctacgatgtt agattcctgc tggtctccga agtccacatc ccatccctca gagacatagt
      301 acatctccct taaagtggct gtttttccac gtctcaccag gtctttacag aagttagcta
      361 catagaccat ctgggcaatt ttaggtatct gtttcacgtt acccaggcta cgctgaccgt
      421 aacggtctcc caggacgtaa taacgtttgt cttcatcgta aattatattg gatgttcccc
      481 gggagggtac tttgagtgca gggacgttgt tctggtttat ctcctctatt atcctctccc
      541 ccagactttt gagtttgtta atggcgatgt cttttctatt ggcaattttg ttgttatgac
      601 tcatcttcac cctcctcact ttcgagtggt atattttcaa atttagctct gcgtgtcact
      661 ttggcaagta cagaatcata ttctgggacg tcggtttctg ccagtaccgc tgcctcccgg
      721 atgataaccg gtaccaggtt ttcaaatatt ttagaacgca tggcctcttc tttagcggct
      781 ctttttgccc ttatatattt ctgaaggttc ctggcgattt tcatggtggc ctgccgtact
      841 tcatggagta tctctggctc gggagctacg ctctgctttc cagtggagag ataaggtacg
      901 ttggtggaga ctatgttgac gaaaacggtt atgggtgcgt tgtccaggtc ccttatgccg
      961 taccgcttcc aatctatgct cttcagtgct tcggttatgg cacagctacc ttggtcgaag
     1021 gttaggggca cccggttggc gaacctcatg atctccgcct ttttctgttc acccaccagt
     1081 ctcccggagt ctcctccata ggagataccg gcttctatca caaaggccac tccaccccgg
     1141 taggtttttg gtttccggct ggt
//
LOCUS       DAOQVW010000571         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_178403_length_1163_cov_1.713899, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000571 DAOQVW010000000
VERSION     DAOQVW010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178403_length_1163_cov_1.713899"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..479)
                     /locus_tag="PKK85_09660"
     CDS             complement(150..479)
                     /locus_tag="PKK85_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26320.1"
                     /translation="MENQIEEKIVEALENVEPWQRVPTSVDGAFLVKTPEKGGNKTIM
                     VEINPLNERRTPMKRRGLFLRQSVELEKFLEVLENESIGELLKAVDDMSAKSVAKTPE
                     GIKTLEI"
     gene            647..>1163
                     /locus_tag="PKK85_09665"
     CDS             647..>1163
                     /locus_tag="PKK85_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HNS26321.1"
                     /translation="MRIVHQDTKKGIIELLPETLDDLWHLSHLIEPGDLLSARTTRRI
                     QDTTGERLRSDRGIKKTFFMGIRVESINFHRYTGKLRATGVIEKGPEDLVPLGSHHTL
                     DLKLKNPVKIQKEKWSRWHLKRIKDAVEASKIPKALVVVIEDDNADIGILRQYGVEYY
                     GPIVGGVSGKRM"
BASE COUNT          351 a          241 c          237 g          334 t
ORIGIN      
        1 tcctagtcca cgggtacctc caatcactgc aatttccata gagttatctt tagatgggct
       61 gtctattaaa tatttttaat agattatctg ggcatctcta aattttctaa atatgatacc
      121 atccctctca ttctgtaact ctatgatttt catatctcca gtgtttttat tccctcaggg
      181 gtttttgcca cagatttagc ggacatatca tccactgcct taagtagttc gccgatgctc
      241 tcgttctcca ggacttccag gaacttttcc agttctacag actgccttaa aaacaggcct
      301 ctacgtttca tgggtgttct acgttcgttt aatggattta tttctaccat tattgttttg
      361 ttacctcctt tttccggagt tttaactaga aatgctccgt cgactgaggt gggcactctt
      421 tgccaaggtt cgacattttc taaagcttct acgatctttt cttcaatctg gttttccatt
      481 ttagtcacca ctataaaggt agtaataact tttatttaaa agttatgatt gagcaggtga
      541 gaaaaaaaac tgagaacaaa aaaattaatt aaaacgtaaa tggagaacaa ataaataaga
      601 atatagtcta ttattaaaca gaataaaata taaagggcat attatcatgc gcatcgtcca
      661 ccaagataca aagaagggga tcatcgaatt attaccagaa accctggatg atctatggca
      721 cctctcccat ttgatagaac caggagacct cctatcagcc aggacaacaa ggcgtataca
      781 ggacacaacc ggggaacgcc tccgtagcga tagggggatt aagaaaacat tctttatggg
      841 catacgcgta gaaagcatta atttccaccg gtacaccgga aaattacggg caaccggtgt
      901 gattgaaaag ggtcctgaag acctggttcc cctgggctcc caccataccc tggatttgaa
      961 gcttaaaaat ccagtgaaaa tacagaaaga gaaatggtcc cgctggcacc ttaaaaggat
     1021 taaagacgcg gttgaagcat ctaaaatacc caaagccctg gtggtggtta tcgaagatga
     1081 caacgcggat attggaatac tgcgccagta tggtgtggaa tattacggac ccatagtggg
     1141 tggagtgtcc gggaagagga tgg
//
LOCUS       DAOQVW010000572         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_179473_length_1159_cov_1.440217, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000572 DAOQVW010000000
VERSION     DAOQVW010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179473_length_1159_cov_1.440217"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /gene="dapA"
                     /locus_tag="PKK85_09670"
     CDS             complement(<1..768)
                     /gene="dapA"
                     /locus_tag="PKK85_09670"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNS26322.1"
                     /translation="MKLEGTIVAMVTPFNSEDEVDQEGMRENINYLIDRGVEGVLAAG
                     TTGESATITHLEQRKMMDILVDEVAGKVKTIAGAGSNSSKESLGLVKYAEAAGADAAL
                     VITPYYNKPQPHGLYEHYKLLTDTTDIPIIVYNVPSRTGTDIDVETIGQVSQLDNVIA
                     IKEASPDLDKVSQIEKKMEELGNRDDFAVISGNDNLTLPMISMGAKGVISVVANVDPQ
                     RMGDMVRYALKGDFTKAKELHYELYHLMKVLFVESNPV"
     assembly_gap    814..823
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<824..>1159)
                     /locus_tag="PKK85_09675"
     CDS             complement(<824..>1159)
                     /locus_tag="PKK85_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNS26323.1"
                     /translation="ILTKPGVLSALTNALARNDINIYGISTGQNSVTLFIDRSAADEA
                     HKILHDVVVENKFLSSLSLDREIAMLTVASQDFIDTPGIITKITEPLHKSNINIVEIS
                     SSQTSVVIFV"
BASE COUNT          235 a          272 c          266 g          376 t
ORIGIN      
        1 gacggggttg gattccacga ataagacctt catcaggtgg tagagttcat agtggagttc
       61 ttttgccttt gtaaaatcgc ctttaagggc gtatctcacc atatcaccca tcctctgggg
      121 atccacattg gccactacac tgattactcc tttagctccc atggatatca tggggagggt
      181 taggttgtcg tttccactta taacggcgaa gtcgtccctg tttcccagtt cttccatttt
      241 cttctctatc tgggagacct tgtccaggtc ggggctggct tctttgatgg ctattacgtt
      301 gtctaactgg gatacttggc cgatggtctc cacgtcgatg tctgtcccgg tccgtgaggg
      361 cacgttataa acgattatag ggatatctgt tgtgtcggtg agtaatttgt agtgttcgta
      421 gagtccgtgt ggttggggct tgttgtagta gggagttatc actaatgctg cgtctgctcc
      481 agcggcttca gcgtacttca ccagtcccag ggattctttt gatgaattgc ttcctgctcc
      541 ggctatagtc ttcaccttac ccgctacttc atccaccagg atgtccatca tcttcctctg
      601 ttccagatgg gttatggtgg ctgattcgcc ggtggttcca gcggccagta caccttctac
      661 gcctctatct atcagatagt ttatgttttc ccgcatgcct tcctggtcta cctcatcctc
      721 cgagttgaag ggggtgacca tggcgactat tgtaccttct agtttcattc caagacactc
      781 cttaccattt cataagcttt cttaccatca ttcnnnnnnn nnnacaaata tcactacaga
      841 agtttgactc gaagagatct ccacgatgtt aatattactt ttatgcagag gttctgttat
      901 tttagttata atacccgggg tatcgataaa atcctgactg gccacggtga gcatggctat
      961 ttccctgtcc agggagaggg agcttagaaa tttattttcc acaaccacat cgtgtaatat
     1021 tttatgagct tcatccgcgg ctgatctatc tataaacagg gtcacactgt tctggccggt
     1081 ggatataccg tatatgttta tatcgtttct ggccagtgca ttggtcaggg ccgatagtac
     1141 gcctggtttg gttaatatt
//
LOCUS       DAOQVW010000573         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_180184_length_1156_cov_2.353315, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000573 DAOQVW010000000
VERSION     DAOQVW010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180184_length_1156_cov_2.353315"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1156
                     /locus_tag="PKK85_09680"
     CDS             <1..>1156
                     /locus_tag="PKK85_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNS26324.1"
                     /translation="EYVDEENKPLAEDSKEYKISELKAGNHNVSLVGRVKRISNVKKF
                     VNRKGREGKLANILIADDTGEMRVVLWTENIKLLKKFQEGAVIRVKGVEIKQGFRNDE
                     AHLNLNSTIEKVSEDDYPDLPPYEEKITDIKDIKEDMEVNIIARVIRIPRVRTFDRNG
                     KEGKFIPLEIQDKSGKTSLMLWNKDTELLEALDIQEGDSIKVLGAQSRLRNGVISLSH
                     FYLGRIIKGDFDVPEYTESVVKIGDAHEMRDVTVFGVVSKIYDTITFVRDDASTGQVR
                     SLEIEDNTGKIRVTLWNEDTKIELKKGDVIKITGGNIEFDDYSGTDYRINTNWNTVIT
                     VNPPLEDDIKKMLEECGKYLKPVKIGDLNTIEDEGEEVDIIGRVINLFEPN"
BASE COUNT          431 a          210 c          270 g          245 t
ORIGIN      
        1 gaatatgtgg acgaagaaaa caagccatta gccgaagatt ccaaagagta caaaatatcc
       61 gagctaaaag ccggcaatca caacgtgagt ttggttggaa gagttaaacg tatttccaac
      121 gtgaaaaaat tcgtcaacag aaagggaaga gaaggaaaat tggccaatat attgatagct
      181 gacgatactg gtgaaatgag agtagttctg tggactgaga atatcaaact ccttaaaaaa
      241 ttccaggaag gcgccgtgat aagggtaaag ggtgttgaaa taaaacaggg attcagaaat
      301 gacgaagcac acctcaacct caactccacc attgaaaagg tctccgaaga tgattatcct
      361 gatttaccac catatgaaga gaaaataaca gatataaagg atatcaaaga ggacatggaa
      421 gtcaacatca ttgccagggt catcaggatc ccccgggtta ggaccttcga ccgaaacggt
      481 aaggaaggaa agttcattcc cctggaaata caggataaaa gtggaaaaac cagtctcatg
      541 ttatggaata aagacacgga actcttagaa gcccttgata tccaggaagg cgattctata
      601 aaagtgttag gagcgcagag cagactgcgg aatggtgtaa tatcactatc ccatttctat
      661 ctgggaagga taataaaagg cgactttgac gtcccagaat atactgaaag cgtcgtgaaa
      721 attggtgacg cccatgaaat gcgtgacgtg actgttttcg gtgtggtaag taaaatctat
      781 gacaccataa cctttgtaag agatgacgcc agcaccggcc aggtcagatc cctggagata
      841 gaagacaaca ccggtaaaat aagggtcacc ttatggaatg aagataccaa aattgagctg
      901 aaaaagggag acgtcataaa aatcaccgga ggaaacatcg aattcgatga ttattccggg
      961 acagattacc gaataaacac caactggaac accgttataa cagtgaatcc cccattagaa
     1021 gatgatataa aaaaaatgct tgaagaatgt ggtaaatacc ttaaaccggt taaaattggg
     1081 gatctaaata ccatcgaaga tgaaggagaa gaagtggata taatcggacg ggttataaac
     1141 ctcttcgaac ccaacg
//
LOCUS       DAOQVW010000574         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_180841_length_1153_cov_4.474499, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000574 DAOQVW010000000
VERSION     DAOQVW010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180841_length_1153_cov_4.474499"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..613)
                     /locus_tag="PKK85_09685"
     CDS             complement(308..613)
                     /locus_tag="PKK85_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26325.1"
                     /translation="MADKKKMTQTSRHHWEKRGFSLHDHPPRVEVLKRNLKHAERELS
                     QVKKSLPRVEAWVRAGVDPLVGPSYDIQLDNLLTKRLNLEAVIRSLQVELMAIEETK"
     gene            complement(761..>1153)
                     /locus_tag="PKK85_09690"
     CDS             complement(761..>1153)
                     /locus_tag="PKK85_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26326.1"
                     /translation="EELRQKAGLMPELFDKEYKAKLKEIKKVENLLLTAEKETEALKT
                     EYFKLEGETLRNLESETYIPYFIELNHLFWDLQARAFQENNPEYDQKLYELSQLMDKP
                     PVLKYMRVARRTRRRPGRVLLPGVRNFL"
BASE COUNT          302 a          258 c          230 g          363 t
ORIGIN      
        1 catttgacct tatgatctta tacctccacc taacaaaccg ggaggattgc agcccagagg
       61 ttaggaaagc cattgataaa ttagttaaaa attatctgga aaaatatgat ctcacaatca
      121 agaagctcat agatcgggag ctctcaggaa ttggaggttt ggggggcaaa tactaatttt
      181 atatggctta ttgggacggt gccccccggt gccttatcca tgaatgaaga acatttttaa
      241 tttaattgtg ggtaaggctc ctatgttccc aattcatagc ccaaaaaaac attaaaatta
      301 ttttattcta ttttgtttcc tcaattgcca ttaattccac ttgcaaggac cggatcaccg
      361 cctccaggtt aagcctctta gtaagtagat tgtcaagttg gatgtcatag gacggaccaa
      421 caaggggatc aacaccggcc cggacccagg cctccaccct gggcaaactc ttcttaacct
      481 gtgataattc cctttcagca tgctttaagt ttcttttaag aacctcaacc cgtggggggt
      541 ggtcgtggag tgagaacccc cgtttttccc agtggtggcg gctggtttgg gtcattttct
      601 ttttatctgc cattcactgg gcctccaggg cttccgctat ccttgttaat aggcttatta
      661 tctgttcttg gtttgctaag aattggagga ttgtttcatt ccttaaatta gattttgcca
      721 taagttaaac accaccacta aaaaaataaa ggaacatgtt ttaaagaaaa ttcctaacac
      781 ctggtagcaa cacccggccg ggtctccttc tggtcctcct agcaaccctc atatatttaa
      841 gaaccggtgg cttatccatc aactgggaaa gttcatataa cttttgatca tactcggggt
      901 tgttttcctg gaaagccctc gcttgaagat cccaaaagag atgattgagc tctatgaagt
      961 agggaatata tgtctcactt tccaagtttc ggagtgtctc accttccaac ttgaagtatt
     1021 ccgtcttcag ggcctcggtt tccttttcag ccgttaagag taagttttca accttcttaa
     1081 tttccttaag cttagcttta tactctttat caaataactc aggcataagg ccggcttttt
     1141 gtcttagttc ttc
//
LOCUS       DAOQVW010000575         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_182048_length_1149_cov_2.113346, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000575 DAOQVW010000000
VERSION     DAOQVW010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182048_length_1149_cov_2.113346"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /gene="ndk"
                     /locus_tag="PKK85_09695"
     CDS             <1..404
                     /gene="ndk"
                     /locus_tag="PKK85_09695"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNS26327.1"
                     /translation="GKIITRLEEKGLKIVAARMLIIDDDLAKEHYGEHKEKPFFQDMV
                     EYITSGPVLAMVIEGNECISLIRKIVGATNPKEADLGTIRGDYAIDTGRNIIHASDSP
                     GSAEREIALFFSEDEICSYTLPDEELIYEEP"
     gene            538..>1149
                     /locus_tag="PKK85_09700"
     CDS             538..>1149
                     /locus_tag="PKK85_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNS26328.1"
                     /translation="MKMRSPIVSVLGHVDHGKTTLLDFIRGSAIAQKEAGGITQHIGA
                     TEIPMEVIENISGAFIQKLDIKDKMPGLFFIDTPGHEAFTTLRKRGGALADLAILIVD
                     INEGFKPQTYEALNILKMYKTPFIVAANKIDKIYGWEVHQNSTFMKSYHEQAGSVQEK
                     LDTSIYELVGILHEEGFESERFDRVTNFARQVSIIPMSAKTGEG"
BASE COUNT          381 a          258 c          264 g          246 t
ORIGIN      
        1 ccggtaaaat aataaccaga ctggaagaaa agggattaaa gatagtcgcc gcccggatgc
       61 tcatcatcga tgatgacctg gcaaaggaac actacggcga gcacaaagaa aaaccattct
      121 tccaagacat ggtggaatat ataacctcag gaccggtact ggccatggtg attgaaggca
      181 acgaatgtat aagtcttatc agaaaaatag tgggcgccac caatcctaaa gaagcagatt
      241 taggaacaat caggggtgac tacgccattg acactggtag gaatatcata cacgcctcag
      301 attccccagg atccgccgag cgagagattg ccctgttctt cagtgaagat gaaatctgca
      361 gctataccct gccagatgaa gaactcatct acgaagaacc ttaaacccca ctattttttt
      421 acgaagctca cccctttctt attttaaaag gatattattt caggtaatgt taaagaatta
      481 ttcaagaaat ggatcaattt ctaacagaac gaatatattc ggaggaacac gattaatttg
      541 aagatgagat cacccatagt ttctgtttta ggtcatgtcg accacgggaa aaccaccctg
      601 ctggacttca tcagaggcag cgccatagcc cagaaggaag cagggggcat aacccagcac
      661 ataggtgcca cagagatacc catggaagtg atcgagaata tcagcggtgc tttcatccaa
      721 aaattggata tcaaggataa gatgcccggc ctgttcttca tagacacccc gggacacgaa
      781 gcattcacca ccctacgtaa aaggggaggt gcactggctg atctggccat attaatcgtg
      841 gacataaacg aaggattcaa gccacagacc tatgaagctt taaacatcct taaaatgtat
      901 aaaacaccct tcatagtggc ggcgaataag atcgataaaa tctatggatg ggaagtccac
      961 cagaattcca cattcatgaa atcgtaccat gaacaggcgg ggagcgtgca ggaaaaactg
     1021 gacaccagca tctatgaact ggtgggaatc ctccacgaag aaggtttcga gtcagaaaga
     1081 ttcgaccggg ttacgaactt cgccaggcag gtaagcatca tccccatgag tgccaagaca
     1141 ggtgaagga
//
LOCUS       DAOQVW010000576         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_182176_length_1148_cov_3.682525, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000576 DAOQVW010000000
VERSION     DAOQVW010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182176_length_1148_cov_3.682525"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..795)
                     /locus_tag="PKK85_09705"
     CDS             complement(262..795)
                     /locus_tag="PKK85_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HNS26329.1"
                     /translation="MDDPEYLRLKKQLSFNLKEKSAIRQLNIQADAFLPVLFSLKFGG
                     DWSFKTRDLEALAVKEKITRYNEDIKEGYTMERVTLLVNPQLIGTEGRVLRLEKCGNK
                     KERELVERPYQVELQAEDIILAELDPKSMEIVLRRIDGPLFFEGSAAYGVSHEMEHLE
                     GQERSGKYLWEFDYRVE"
     gene            complement(851..>1148)
                     /locus_tag="PKK85_09710"
     CDS             complement(851..>1148)
                     /locus_tag="PKK85_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HNS26330.1"
                     /translation="VLIDHLEKVHGCQVVGTTPHLSNRPLCQRDIEKYIDEAEVMLTE
                     LKAAAVDVATKDALEAGMEVVYCDNIPLSIRKEDDLDSAIIQVVDSAIQDFQSR"
BASE COUNT          269 a          301 c          251 g          327 t
ORIGIN      
        1 gcatcttgtc cattccctta ggaccaagag tggttctgac aattcctgcc agggtctttc
       61 ctgccattat gttcattctt tgagcgtctc gtcccaaaag tcttgaggta ccttcaggta
      121 gtattataat ctgttggcct tgtccgccta actgtgccac tatatcatct ccattttctt
      181 acagtaattt agaggtgggt tagaggttat ataaatagtt tgcccagggg gggtggtggt
      241 tcagatccta ggaaaaaggg gttactccac ccggtaatca aactcccata aatacttacc
      301 cgatctttcc tggccctcca gatgttccat ctcatgggaa acaccataag ccgcggatcc
      361 ttcaaaaaaa agcggaccat caatacgtct aagtactatt tccatggatt ttgggtccaa
      421 ttccgccaag attatgtcct ccgcctggag ttccacctga tagggtcttt caaccagttc
      481 ccgctccttc ttgttgccac atttttccag gcgcagcacc cgaccctcgg tgcctattaa
      541 ttgggggttt accaggaggg tcaccctctc catagtgtag ccctccttta tatcttcatt
      601 gtagcgggtg atcttctctt taactgccag ggcctccaga tcccgtgttt tgaaactcca
      661 gtccccccca aatttcaggg aaaaaagcac aggaaggaag gcatccgctt ggatattcaa
      721 ctggcgaatg gcacttttct ccttcaggtt gaaagaaagc tgttttttca aacgaagata
      781 ctctggatca tccaatctat caccaaaaaa aatatagaaa aaaggggatg gtttaagcca
      841 cggtgcttat ttatctggac tggaagtcct ggatggcact gtccaccacc tggattatgg
      901 cactgtccag gtcatcctct ttccggatgc tcaggggtat gttatcacag tacaccactt
      961 ccatacccgc ctccagggca tccttggtag ccacgtccac cgccgctgcc ttgagttcgg
     1021 tgagcattac ctcggcttca tcgatgtact tttcaatatc ccgttgacat agggggcggt
     1081 tggaaaggtg gggtgtggta cccactacct gacagccatg aaccttttcc agatggtcta
     1141 ttaagacc
//
LOCUS       DAOQVW010000577         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_182198_length_1148_cov_3.175663, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000577 DAOQVW010000000
VERSION     DAOQVW010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182198_length_1148_cov_3.175663"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..600
                     /locus_tag="PKK85_09715"
     CDS             19..600
                     /locus_tag="PKK85_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HNS26331.1"
                     /translation="MEDYAIILDFLPLGYVDEGTTTYKRKPVAQAIGTEEFTLLELSP
                     KDGIALNILQKVYIGAGKRDEISRVNRRIPYEKLTSTAKIELSYIIEEIVKEKEDKFV
                     HFFNESGPISLRLHQIELLPGIGKKHMADILEARKKGPFKSFDDIKKRVPMISDPMKL
                     ISKRIVMELESHEAKGKRKYVIFTRPPKRKPQP"
     gene            606..>1148
                     /gene="rsmA"
                     /locus_tag="PKK85_09720"
     CDS             606..>1148
                     /gene="rsmA"
                     /locus_tag="PKK85_09720"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNS26332.1"
                     /translation="MLYRETRDILKRHQIQLNKRKSQHYLVNSSILDRIIQKADLSIS
                     DTVLDIGAGIGTLTIPLSEEVEKVVAIERDPHIADVLRERLKNEKITNVDVLEGDATR
                     LKLPAFNKVVSNLPYQISSPITFRLLEYDFEFAILMYQLEFAERMVAKPGDSKYSRLS
                     VMINLKANVEKLFTVPKERLY"
BASE COUNT          384 a          226 c          242 g          296 t
ORIGIN      
        1 ctggagatgt atggtcatat ggaagattat gcaataatac ttgattttct cccactggga
       61 tatgtggatg agggtacaac cacttataaa cgaaaaccag tagcacaagc cataggtact
      121 gaagagttca cccttctaga gctgagtccc aaagatggaa tagcacttaa catacttcaa
      181 aaggtgtaca ttggagccgg gaaaagggat gaaatatcta gggtaaaccg ccggatcccc
      241 tacgaaaaat tgacttccac cgctaaaatt gaacttagtt acattataga agagattgtg
      301 aaggagaaag aagataaatt tgtccatttc ttcaatgaat ccggaccaat atctcttaga
      361 ttacaccaaa tcgagctttt gccaggtatt ggtaagaaac atatggcaga catccttgag
      421 gccaggaaaa aaggtccttt taaaagtttt gacgatataa aaaagagggt tcccatgata
      481 tccgacccta tgaaactcat ctccaaacga atcgtaatgg agctggagtc ccatgaagcc
      541 aagggtaaaa gaaaatacgt cattttcacc cggcccccta aaaggaaacc ccaaccctga
      601 aggaaatgtt atacagggaa acaagagata tacttaaaag acaccagatt cagctgaata
      661 aacgaaaaag tcagcattat ctggtgaatt cttctatttt agatcggatt attcagaaag
      721 cagacctctc tatctctgac actgttctgg atattggtgc cggaattggt accctcacaa
      781 ttcccctttc agaagaagtt gaaaaggtag tggccataga acgggacccc cacatagccg
      841 atgtcctcag agaaaggctg aaaaatgaaa aaataactaa tgtcgatgtg ttggagggtg
      901 atgcaactcg tttgaagtta ccagccttca acaaagtggt ttctaatctt ccttatcaga
      961 tttcttcacc aataaccttt cgacttttgg aatacgattt tgaatttgca attttaatgt
     1021 atcagctgga gtttgcagag aggatggtgg ccaagccagg ggattcaaaa tactcccggc
     1081 tatcggttat gatcaacctg aaggccaatg tagagaaatt atttacagtc cctaaggagc
     1141 gtttatac
//
LOCUS       DAOQVW010000578         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_182713_length_1146_cov_3.516957, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000578 DAOQVW010000000
VERSION     DAOQVW010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182713_length_1146_cov_3.516957"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1146
                     /locus_tag="PKK85_09725"
     CDS             <1..>1146
                     /locus_tag="PKK85_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNS26333.1"
                     /translation="GKAVVFATGAETEFNQIAALTQELKEEPSPLQKELNRLTRVIAV
                     IAILLGLCLFAINVWLVELPLTVAFIFAIGLTVANVPEGLLPTVTLSLAASVQKMAQK
                     NALIKRLANVETLGSTTIICTDKTGTLTRNAMTVRQVWVPYGVVEVTGTGYEPDGSFL
                     RDGKVVNHHEIREIKLLMRAASFCNDAQLIAPQGLDEKWRVLGDPTEAALLVAAEKIG
                     FHWQEEIKNMPRIVELPFDSQRKSMSSIHQRPGQRVAYVKGAPKKIIGLSSHISVDGN
                     PHLLSKEGKKDIVKIHDKLASQGLRILAMAYRNLPPELKDYTPEEVETDLTLVGMVAM
                     QDPPRPEVHDAVKDCQEAGIRIIMITGDYGLTAESIAREVGIIRGEHC"
BASE COUNT          315 a          281 c          297 g          253 t
ORIGIN      
        1 gggaaggctg ttgtgttcgc aacaggggct gaaaccgagt tcaaccaaat tgcagcactc
       61 acccaggagt tgaaggagga acccagcccc ctccagaagg aattaaatcg tctcaccaga
      121 gtaattgccg taatcgccat tttactaggc ttgtgtctat ttgcaatcaa tgtatggctg
      181 gtggaactac ccctgaccgt ggccttcatc ttcgccattg gcctcaccgt ggccaatgtt
      241 ccggagggct tgctacccac tgtaacatta tcactagctg cttctgtaca gaaaatggcc
      301 cagaaaaatg cacttattaa acgtctggcc aatgtggaaa ccctgggatc caccaccatc
      361 atctgcaccg acaaaaccgg caccctcacc aggaatgcca tgactgtgcg ccaagtctgg
      421 gtaccctacg gggtggtgga agtcactggt actggttatg aacctgatgg gagcttttta
      481 cgtgatggta aagtggtgaa ccaccacgag atccgggaga tcaaactgtt gatgcgggcg
      541 gcctctttct gtaacgatgc ccagttaata gcaccccagg ggcttgacga aaaatggcgg
      601 gttttgggag accccaccga agcagcacta ctggtggcgg ctgaaaagat tggattccac
      661 tggcaggagg aaattaaaaa tatgccccgg attgtggagt tacccttcga ttcccagcga
      721 aaatccatga gcagcatcca tcaaaggcca ggtcaacgtg tagcatatgt taaaggggct
      781 cctaaaaaga taattggcct gtccagccat atttctgtag atggtaaccc acatctacta
      841 tcaaaggaag gaaaaaagga tatagtgaag attcatgata aactggcctc ccagggactg
      901 cgaatactgg ccatggccta ccgcaacctc cccccggaac tgaaggatta cacaccggag
      961 gaggttgaaa cggacctgac tttggtgggg atggtggcca tgcaggatcc accccgacca
     1021 gaagttcatg atgcagttaa agactgtcaa gaggcaggta tacgtatcat aatgatcacc
     1081 ggtgattatg gactcacagc cgagtccata gcccgtgaag taggtataat taggggtgaa
     1141 cactgt
//
LOCUS       DAOQVW010000579         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_183572_length_1143_cov_3.585478, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000579 DAOQVW010000000
VERSION     DAOQVW010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183572_length_1143_cov_3.585478"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKK85_09730"
     CDS             complement(<1..307)
                     /locus_tag="PKK85_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNS26334.1"
                     /translation="MIDAHIHADCRPYEDFEKMAVAGVEKAVTLAHDPLPMSNSGVVM
                     DHFHRMLNHDRERAAANGIELFVALGVHPRSICPDVDLVLEELPLLLEDQRVVALGEV
                     "
     gene            441..845
                     /locus_tag="PKK85_09735"
     CDS             441..845
                     /locus_tag="PKK85_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26335.1"
                     /translation="MSAQLAPLSEIEDALRSLELVETNNSQFPLMSRVMIGYDPGEPE
                     DPDPQNWKNVMLEIGLERTGDEDFEWYWEISIRDFSTTEFIIKRHNTGLQAHYKPSCS
                     GCFGLPMEIEGKKPEDFVQLLIKDIAEHFNLD"
     gene            902..1057
                     /locus_tag="PKK85_09740"
     CDS             902..1057
                     /locus_tag="PKK85_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26336.1"
                     /translation="MDVEEMAKKATLKELKEIAEKHDIKLGRCPTKLKIAQLIPRSEL
                     EELVSKK"
BASE COUNT          359 a          231 c          259 g          294 t
ORIGIN      
        1 ccacctcccc cagtgccacc acccgttggt cctccaagag gaggggtaac tcttctagga
       61 ccagatccac atcgggacat atactccggg gatgcactcc cagggccacg aaaagttcaa
      121 ttccattggc agcagctctt tcccggtcat ggttcagcat acggtggaaa tggtccatta
      181 ccaccccgga gttactcatc ggcagggggt cgtgagccag ggtcacggcc ttttcaaccc
      241 cagccactgc catcttttca aagtcttcgt agggccgaca atcagcatgt atatgtgcat
      301 cgatcataac aatcaccacc aggcatatat tcagtggatt tgtatgttgt tcccagagaa
      361 tataataagg ttattaccag ttcagtacta ctaaaaaata tggggcaaaa aattacacat
      421 ttaaaatgga gatgtatttt atgagcgctc agttagcacc gttaagtgag atagaggatg
      481 cattaagaag tttggagttg gtagagacca ataattctca attccccctt atgagtcggg
      541 tcatgattgg atatgaccct ggtgaaccgg aagatcctga tcctcagaat tggaagaacg
      601 tgatgctgga gattggattg gaacgtactg gtgatgaaga ttttgagtgg tactgggaga
      661 tcagtatcag ggatttttcc accaccgaat ttattattaa aaggcacaat accgggctac
      721 aggcacatta taaaccaagt tgcagtggat gcttcggttt accaatggaa attgagggta
      781 agaaaccaga agacttcgtt caactcttaa taaaggatat agctgaacat tttaatttag
      841 attaaatttc tatctcatga taatggatat catgatagga caaaccgaaa atggtgttat
      901 tatggatgta gaggaaatgg ctaagaaggc cacactgaag gaactgaagg aaatcgcaga
      961 aaaacacgat ataaaactgg gccgttgtcc caccaaactg aagatagccc agttgatacc
     1021 tagatcagaa ctggaggaac tggtcagtaa aaaataatcc catcaaaaaa aaggaaaaca
     1081 aggttaggac tgttatttcc tggcttcaaa ttctttgaac tctttttttc ctgataaaag
     1141 cag
//
LOCUS       DAOQVW010000580         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_183903_length_1142_cov_2.843606, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000580 DAOQVW010000000
VERSION     DAOQVW010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183903_length_1142_cov_2.843606"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1142
                     /gene="serA"
                     /locus_tag="PKK85_09745"
     CDS             <1..>1142
                     /gene="serA"
                     /locus_tag="PKK85_09745"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNS26337.1"
                     /translation="VNAPESTSITVAEHTMGLMLSLARRIALADKSVKEGKWDKSAFM
                     GMELNGKTLGIVGMGRIGSQVASRCKAFGMDILVYDPYITPEAAAEIGVEVVDLDGLL
                     RAADVITIHVPLTPETKHFIAKEEMEIMKDTAFIINCARGGIINEDDLYQALLDGKIG
                     GAALDVFEQEPPKDNPLLNLNTAIFTPHIGASTREAQRDAAIIVAKDVKEVLKGGSPK
                     NVLNMPVIDPKTFQQIKPYLNLAEKMGKFLIQTAEGNINELEITYCGELSRIPNHDII
                     TRILLQEVLNPILTEPVNLVNAATVAQNRGIIITEGKRSEAGGYKNLIKVDMKADHNE
                     VSVEGIFIQEPKIVMINDYPVDVETKGTMLIAKYKDIPGIIGFIGT"
BASE COUNT          374 a          242 c          266 g          260 t
ORIGIN      
        1 tggttaatgc cccggaatcc acttccataa ctgtggccga acatacaatg gggctcatgc
       61 tttcccttgc ccgtagaata gccctggctg ataaatcagt taaggaaggc aaatgggata
      121 aaagtgcatt tatgggcatg gaattaaacg gcaaaaccct gggaatagtg gggatgggtc
      181 gtattggaag tcaggtggcc agtcgttgca aggcctttgg tatggatatc ctggtttacg
      241 acccatacat cacaccagag gctgctgccg aaataggggt agaagtagtg gatttggatg
      301 gtctcctgag agcagctgat gttataacta tccatgtgcc cctaactccc gagaccaagc
      361 acttcatagc taaagaagaa atggagatca tgaaggacac ggcatttata atcaactgtg
      421 ccaggggggg tatcataaat gaagatgacc tttatcaggc cctactagat ggaaaaattg
      481 gtggagccgc tttagatgta tttgaacagg aaccacccaa ggacaatccc cttctaaacc
      541 taaatactgc gattttcacc ccccacatcg gggcatctac ccgtgaagca cagcgcgatg
      601 cagcaataat cgtggctaaa gatgttaaag aagttttaaa aggagggtca cctaaaaacg
      661 ttctcaacat gccagttatt gatcccaaaa ccttccaaca gatcaaacca tacctcaacc
      721 tggcagaaaa aatgggaaaa tttttaatac agactgctga aggaaatatt aacgaactgg
      781 agattaccta ctgtggagaa ctgtcaagga taccaaatca tgatataata accagaatac
      841 tgctccagga ggttttaaat cccatactca ccgaaccagt gaacctggta aacgccgcca
      901 ctgttgccca aaataggggc ataatcatca ccgaaggaaa acgcagcgaa gcagggggat
      961 ataaaaatct gataaaggtt gatatgaagg ctgaccataa cgaggtgagt gtggagggaa
     1021 ttttcatcca ggagcccaaa atcgtaatga taaatgacta ccctgttgat gttgaaacta
     1081 aaggcaccat gctcatagct aaatacaagg acataccagg aataattgga ttcattggta
     1141 cc
//
LOCUS       DAOQVW010000581         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_184932_length_1138_cov_6.196676, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000581 DAOQVW010000000
VERSION     DAOQVW010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184932_length_1138_cov_6.196676"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKK85_09750"
     CDS             <1..315
                     /locus_tag="PKK85_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26338.1"
                     /translation="DYGKRGIGNLSVSGTSGGQGNRMLRCKTCGYRFSETRGTALHNS
                     KIEHEKAVSVMEHLSEGCGIRKTSRLTGVSLGTVARLLKKAGPHAQLIHDELVSFSPS
                     DK"
     gene            278..1075
                     /locus_tag="PKK85_09755"
     CDS             278..1075
                     /locus_tag="PKK85_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26339.1"
                     /translation="MMNWFLFPPQTNEVQFDEKWSFVGKKDKNCDPFDPNDEKYGDNW
                     DHMAFDPEHRLVISVIPGKRTAENIHKLIEDFIKRTRGKLMRLITTDKYMPYKEAIRI
                     GYGRLVNPERTGKRGRPKKPYWEVPSGLIYAMVKKTLSRGRISKVETELVFGTEEELK
                     RALKRSKVSKKVNTSFIERQNGTDRNRNSRKIRKSLSFSKKPEEHEAMTFFCLYSYNF
                     CWPIRTLQIQDVQGGHSIKRTPAMAAGLTDHVWTIEEWIRFPAVQLC"
BASE COUNT          384 a          205 c          257 g          292 t
ORIGIN      
        1 gactatggaa aacgaggaat tggaaatctc tcggtaagtg gaacctctgg aggtcaagga
       61 aaccgaatgt tgagatgtaa gacttgtgga tatcggttct ccgaaactag aggtactgca
      121 cttcacaact cgaaaattga gcatgaaaaa gcagtttcag tcatggaaca tctatctgaa
      181 ggatgtggta tacggaagac cagtaggtta acaggtgtga gtttagggac ggtcgctaga
      241 cttttaaaaa aggcagggcc ccatgctcaa ttaatccatg atgaattggt ttctttttcc
      301 ccctcagaca aatgaggttc aattcgatga aaagtggagt tttgtcggta aaaaagataa
      361 gaattgtgat cctttcgacc caaatgatga aaaatatgga gataactggg atcacatggc
      421 ttttgatcca gaacatcggt tagtcatttc agtcatcccc ggaaaacgaa ctgcagagaa
      481 catccacaaa ctcattgaag acttcattaa aagaaccagg gggaaattaa tgagattgat
      541 aacgacggac aagtatatgc catacaaaga ggccattcgt atcggatacg gcagactggt
      601 taatcctgaa agaactggta aacgtggacg accaaaaaag ccgtattggg aagtcccttc
      661 tggattgatt tatgcgatgg ttaaaaaaac tctttccaga ggaagaatat ccaaggtgga
      721 gactgaactt gtctttggaa cagaggaaga gttaaaaaga gcattaaaac gctcaaaagt
      781 tagcaaaaag gtaaatactt catttatcga aaggcagaat ggaaccgaca ggaatcggaa
      841 ttctcgaaaa attcggaaat cactttcatt ttctaaaaaa ccggaggaac acgaagctat
      901 gacgttcttt tgcttatata gttacaattt ttgttggcca attcgaactc ttcaaattca
      961 agatgttcaa gggggtcatt caataaaacg aacacctgca atggcggcag gtttaacaga
     1021 tcacgtatgg actatcgaag agtggattcg atttcccgcc gtgcagttat gttagatcac
     1081 cacccaaggt tttaatcatc actatttcat ctaaatgggg acgtccacca tcaatttt
//
LOCUS       DAOQVW010000582         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_187935_length_1128_cov_1.992544, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000582 DAOQVW010000000
VERSION     DAOQVW010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187935_length_1128_cov_1.992544"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKK85_09760"
     CDS             complement(<1..225)
                     /locus_tag="PKK85_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26340.1"
                     /translation="MDEFFQKLREIQKKERNLSGLAPVGENFYQNISNYFNVLMKKID
                     DNPFSFESYLLRDAQRITAEICERREHKISN"
     gene            complement(228..962)
                     /gene="pcn"
                     /locus_tag="PKK85_09765"
     CDS             complement(228..962)
                     /gene="pcn"
                     /locus_tag="PKK85_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030337 - DNA polymerase processivity
                     factor activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proliferating cell nuclear antigen (pcna)"
                     /protein_id="HNS26341.1"
                     /translation="MFKAVLSDSNILKTSFDAISSIVDEVQMQADGDGLKLDALDRSH
                     ITFVHLELKSDVFDVYKCNEPLKINVDTEELMKVLKRAKLDDMVEISVDEGNLIITFE
                     GEARRTFKIRLIDIEYEAPSPPRLEYPTEFEIPFNLLKNSIQDIDIVSDKISLIVDQD
                     KFIASAEGEFGEATIEYVHGEKIGDKAKSVFSLEKIKEMLKADKFSETAVLKLGNDMP
                     LNLSLKMPADEGELSFLLAPRIESEE"
BASE COUNT          306 a          241 c          173 g          408 t
ORIGIN      
        1 gttggaaatt ttatgttctc ttctctcaca tatctctgca gtaattctct gggcgtcgcg
       61 aagtagatag gactcaaaag agaagggatt atcatctatc ttcttcatga gtacgttaaa
      121 atagttagaa atattttgat aaaaattttc tcccaccgga gctaacccac ttagattacg
      181 ttcttttttt tgaatttctc ttaacttctg gaaaaattca tccaacccta ttcctcactt
      241 tctattctgg gagctaaaag aaagcttaat tcaccttcat ccgctggcat cttcagtgaa
      301 aggttaagag gcatgtcatt acccagtttc agcacagctg tctccgagaa cttgtccgcc
      361 ttcaacatct ccttgatctt ctccagggaa aacacggatt tggccttatc tcctattttc
      421 tcaccgtgga cgtattctat ggtggcttct ccaaattctc cctctgcaga ggctatgaat
      481 ttgtcctgat ccacgattag ggatatttta tccgatacta tgtctatgtc ctgtatggaa
      541 tttttaagga ggttgaaggg tatttcaaat tcagtgggat actccagtcg aggtggactg
      601 ggggcttcat actcgatatc tatcagcctt attttaaagg tccttctagc ttctccttca
      661 aaagtaatga ttaaattacc ctcatcaacg gaaatttcca ccatatcatc gagtttagcc
      721 ctttttagga ctttcatcaa ttcttcagtg tccacattga ttttaagagg ttcattacat
      781 ttatatacat cgaatacatc agatttaagt tccaagtgta cgaatgtgat atgagaacgg
      841 tccagtgcat cgagttttaa tccatcaccg tctgcctgca tctgcacttc atccactata
      901 gaagaaattg catcaaaact agtttttaaa atgtttgaat cacttaaaac tgccttaaac
      961 attttatcct ccttataata ttttttacac atccattctt aaaattttat ttattaatat
     1021 actatttatt ctttttatcc atactctcca ttttttgatc atgatcagaa tttccatgtg
     1081 catcctttac atgattttta tgagcatcct ttacatgatc atcctttg
//
LOCUS       DAOQVW010000583         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_189232_length_1123_cov_2.266854, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000583 DAOQVW010000000
VERSION     DAOQVW010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189232_length_1123_cov_2.266854"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKK85_09770"
     CDS             complement(<1..418)
                     /locus_tag="PKK85_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrocarbon binding protein (contains V4R
                     domain)"
                     /protein_id="HNS26342.1"
                     /translation="MNGEIEEIRGTFKPELIPAATGGNIDDYEKALHTLMKFVGSMSS
                     ALEQVSGRGANAIVYQAGKRMGHEASKLMEKTDDLEKAMGELSEVLGQEFYFEMWKPS
                     SQDNYTVERGDETEVKLLFMDCVVRQTLRRTGLPQKG"
     gene            complement(431..682)
                     /locus_tag="PKK85_09775"
     CDS             complement(431..682)
                     /locus_tag="PKK85_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNS26343.1"
                     /translation="MVEIVIDEDACVGCGSCVEDCPNDVYKMDEAKNKTKVVKVEDCM
                     ACLSCHEICPAQAMEHQDIHVAKRLYIDRSVKDAINRII"
BASE COUNT          270 a          244 c          209 g          400 t
ORIGIN      
        1 gtcctttttg gggtagtccg gttcttctta gggtttgtct taccacgcag tccatgaaca
       61 atagttttac ctctgtttca tctccccttt ccactgtgta gttgtcctga ctggatggtt
      121 tccacatttc gaagtagaat tcctgaccta agacttcgct tagttctccc atggccttct
      181 ccaggtcatc agttttttcc atgagcttac ttgcttcatg tcccattctc tttccagcct
      241 ggtatacgat ggcgttcgcc ccccttcctg atacctgttc cagggcgctg gacatggatc
      301 ccacgaattt catcagtgtg tgtagggctt tttcataatc atcgatattt cctccagttg
      361 cggctggaat taattctggt ttgaaagtgc ctcttatttc ttctatttct ccattcatcc
      421 ttccatcccc ttatattatc ctgtttatgg cgtccttcac gctccggtct atatagagcc
      481 ttttagcaac gtgtatatcc tggtgttcca tggcttgggc cgggcagatt tcatggcagg
      541 aaagacaggc catacagtcc tctactttta ctactttggt tttgtttttg gcttcatcca
      601 ttttgtagac gtcattcggg cagtcctcca cgcaagaacc gcagcccaca catgcgtctt
      661 cgtctatcac tatttctacc atttaaaaaa cctctttcgg attttttcta tgttacttag
      721 tatggggtaa agatttattt tatttaacca aatcttaacc aagtttttct aatgaatttt
      781 ctctaaaaaa tgttactatg aataaagttc tatcgcccac ttactatata atattatttg
      841 cataacttaa gaccgaattt ccctgtttca caaaaaaatc aattttccta acttcaaggt
      901 gagaatcatc ccattagaga tgggtttaat aatttgggaa tttgaaaagt ggttctcatg
      961 aaactcaata actaaaaaaa attaggggat ttagaaggaa ttggtggaga ttaacttatt
     1021 ctagaagtaa ttgatggata tcaatctgtt ctagaaagaa ctggtggaga ttaacttgca
     1081 gtgggttatg tatgctgctg atttcgctcc tggggggtaa cag
//
LOCUS       DAOQVW010000584         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_189323_length_1123_cov_1.754682, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000584 DAOQVW010000000
VERSION     DAOQVW010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189323_length_1123_cov_1.754682"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1123
                     /locus_tag="PKK85_09780"
     CDS             <1..>1123
                     /locus_tag="PKK85_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS26344.1"
                     /translation="DFDQEKILSFKELKEYSDKTANFFKNCGIKKGDKVMLTLKSHYE
                     FWFCILALHKLGAITIPATHMLKTRDIVYRVESAGIKMVVCIAEDGVPEYFDEADEQL
                     KDVNLIKALVGDDERPNWINFRSEIEKASPEFERPTGDQKTENNEPMLVYFSSGTTGL
                     PKMVEHDYIYPLGHIITAKYWQNVKEEGLHYTVADTGWAKAMWGQIYGQWIVGSAVFV
                     YDYERFDAARMLEKASKYGVTTFCAPPTIYRFLIKEDLSKYDFSTLQYAVTAGEPLNP
                     EVYNKFYEFTGLRLMEGFGQTELVVCIVNFPWMEPRPGSMGKPSPGYDIKLMDKDGNI
                     CDVGEEGEIVIETSRQEPPGLFNGYYLDPEKTAEVWHDDY"
BASE COUNT          365 a          248 c          264 g          246 t
ORIGIN      
        1 tgattttgat caggaaaaga tcttaagttt taaggaactt aaagaataca gtgacaaaac
       61 agccaatttc ttcaaaaact gcggaataaa gaagggagat aaggtgatgc tcaccttaaa
      121 aagccactat gaattctggt tctgcatcct ggccctgcat aaactggggg ccatcaccat
      181 ccccgccaca catatgctaa aaaccaggga catcgtgtac agggtggaaa gcgccggtat
      241 caagatggtg gtatgcatag ccgaagacgg ggttccagag tacttcgacg aggcagacga
      301 acaactgaag gatgtaaacc ttattaaagc actggtggga gatgatgaaa gaccaaactg
      361 gatcaacttc cgcagtgaaa tcgagaaggc gtcacctgaa tttgaacgcc ccaccggaga
      421 ccagaaaaca gaaaataacg agcctatgtt ggtatatttt tcttcaggca caactggcct
      481 ccccaaaatg gttgaacacg attatatata tccactggga catattataa cggctaaata
      541 ctggcaaaat gtaaaggaag agggtctgca ctacactgta gcagacaccg gatgggctaa
      601 ggccatgtgg ggacagatct atggacaatg gatagtcggg agcgccgtct ttgtgtatga
      661 ctatgagcgc ttcgatgcag cccgtatgct ggagaaagca tcaaaatatg gtgtcaccac
      721 cttctgcgct ccaccaacca tctacagatt cctcataaaa gaagacctat caaaatatga
      781 cttttcaacc ctacaatacg ccgtgactgc aggcgaaccc ttaaaccccg aggtatacaa
      841 taaattctac gaattcaccg gtttgagatt gatggaaggc ttcggccaaa ccgaacttgt
      901 ggtatgcata gtcaacttcc cctggatgga gcccaggcca ggttccatgg gaaaaccatc
      961 cccgggatac gatataaaat taatggacaa agacggtaac atctgcgatg ttggtgaaga
     1021 aggagaaata gtcatcgaaa ccagtagaca ggaacctcca ggacttttca atggatacta
     1081 ccttgatcca gagaaaacag ccgaagtctg gcatgatgat tat
//
LOCUS       DAOQVW010000585         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_189436_length_1122_cov_2.824742, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000585 DAOQVW010000000
VERSION     DAOQVW010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189436_length_1122_cov_2.824742"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKK85_09785"
     CDS             complement(<1..910)
                     /locus_tag="PKK85_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNS26345.1"
                     /translation="MDLLDDTGLCPLCETSWEPAQLKEHLEENLHRYQEAKDDLNRLD
                     LLSNEIIDCINRLKNSLDEVKKARKEWKLVDNYLELDVWGAELDKLSEKIGDNDSGKI
                     DLHVPENLSQIVGELLEISKNKSSPPSSEQTSWDHLTRLEENLKNLEIAQEDCKIQYG
                     SYQRAEILLQAFEDSRKEVLNELYSDIKDRFVELYRELHGIDEAEFEAALTSAGAGID
                     FQVDFHGRGEHPPHALHSEGHQDSMGICLYLALAERLTQGYIDLIILDDVMMSVDAPH
                     RRRICHLLADFFKGKQFFITTHDRIWA"
BASE COUNT          306 a          236 c          197 g          383 t
ORIGIN      
        1 tagcccagat acgatcatga gtggttataa aaaactgttt ccctttgaag aaatcagcca
       61 ataagtgaca gatcctacgc cgatgaggtg catccacact catcatcaca tcatcaagaa
      121 taatcaagtc aatgtatcct tgagtcaacc tttcagccag tgccaagtac aaacagattc
      181 ccatactatc ctgatgtccc tcactatgca gagcatgtgg ggggtgttct ccgcgtccgt
      241 ggaagtctac ctggaagtct ataccagcac ctgctgaggt taatgcagct tcgaattctg
      301 cttcatcaat gccatgtagt tctctatata gttctacgaa tcggtctttg atatcagaat
      361 agagttcatt taaaacttct ttacgggagt cttcaaaggc ctggagaaga atttcggctc
      421 tttgatatga accatattgg attttacaat cttcttgggc tatttctaag ttcttcagat
      481 tctcctctaa tcgggtgaga tgatcccagc tggtttgctc tgaagatggt gggcttgatt
      541 tgttcttaga tatttccagt aactcaccaa caatttgtga aagattttca ggcacgtgta
      601 aatctatttt tccagaatca ttatccccga ttttttcact tagtttatcc agttctgctc
      661 cccaaacatc cagttcgagg taattatcta ccagtttcca ttctttccta gcctttttta
      721 cttcatctaa actattcttc aatcggttga tacaatcaat aatttcatta gaaagaagat
      781 ccaatcggtt caagtcatcc ttggcttcct ggtacctatg taagttctct tctaagtgtt
      841 ctttaagttg ggctggttcc caactggttt cgcataatgg gcaaaggcca gtatcatcta
      901 ataaatccaa tccccattcg gttagtttaa gtcttttaac tgcacctttc agcagaagat
      961 attagatatc ttaaatgata acttgttaaa ctcattttct ttattcccca gttcttcact
     1021 ggaagaaatg tttattaaat taattaaatc attttttaat agaatagggt taacactagt
     1081 ttctggacgc attgcagggg ggtggatttc atttttcaat aa
//
LOCUS       DAOQVW010000586         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_190565_length_1118_cov_3.322672, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000586 DAOQVW010000000
VERSION     DAOQVW010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190565_length_1118_cov_3.322672"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>1118)
                     /locus_tag="PKK85_09790"
     CDS             complement(147..>1118)
                     /locus_tag="PKK85_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNS26346.1"
                     /translation="GSTATTGGKSELLVGEVLSDGYRDQVYLSTKLPCWLVQEKEDLD
                     HYLDQQLERLRTDRIDFYLLHGLNQKTWENLSNLDVLEFLDRTLEDGRVGYAGFSFHD
                     ELDVFKPIVDSYSWSFVQIQYNYMDQDFQAGRAGLEYIKARYMGSVIMEPLRGGCLTK
                     NVPAVIQDIWDRAPVKRTLAEWGLRFLWDQEEMDLVLSGMSTMEQVEENIRIASEGCS
                     SSLSSLEKELIQEVREAYQSRMHVGCTGCGYCMPCPAGVDIPLNLNLLNDVYIYQNME
                     KPSGNYSFLKAKGMSASYCTDCGECEEKCTQQIPIREYLKETAKTFE"
BASE COUNT          279 a          299 c          233 g          307 t
ORIGIN      
        1 tatcatcaat cacgatgatt ggtggtaact tggatacgca atcacaaacc tctatatttc
       61 tatgatcaga aataaaaaca gcctgctaag tactgattcc tacttagagg gtgcttccca
      121 gagatactaa atgaattcca tattttttat tcaaaggtct ttgcagtctc tttcaggtat
      181 tccctgatgg gtatctgctg ggtgcacttc tcttcacact ccccacaatc tgtacagtag
      241 gaagcactca ttcccttggc ctttaggaag gaatagttac cacttggttt ttccatgttc
      301 tggtagatgt aaacatcatt taaaaggttc aaattcaggg gtatgtccac cccggcaggg
      361 cagggcatgc aataaccaca ccctgtgcag cccacgtgca tccgggactg gtaggcctcc
      421 cggacttcct ggatcagttc cttctccagg gaggataggc tactggagca accttcactg
      481 gcaattctta tattctcttc aacctgttcc atggtgctca taccactcaa gacaagatcc
      541 atctcttcct ggtcccataa gaatcgtaaa ccccactcag ccagtgtcct tttcaccgga
      601 gccctatccc agatgtcctg gatcacagcc gggacatttt tggttagaca gcctccccgg
      661 aggggttcca tgatgacact acccatgtac cgggccttta tatattccaa tccagctcta
      721 cccgcctgga agtcctgatc catgtagtta tactgtatct ggacaaaact ccaggaataa
      781 gagtctacaa tgggcttgaa tacatctaat tcgtcgtgaa aggaaaaacc agcgtatcct
      841 acccggccat cttccagggt tcggtccaga aattcgagaa catccaggtt gctcaggttt
      901 tcccaggtct tctggttcag accatgcagt agataaaaat cgatccgatc agttcgcagc
      961 ctttccagct gttgatccag gtaatgatcc aggtcttctt tttcctggac caaccagcag
     1021 ggtagtttgg tggataggta cacctggtcc cggtaaccat cagagagcac ctctcccacc
     1081 aataattcac tcttacctcc agtggtggct gtggagcc
//
LOCUS       DAOQVW010000587         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_190765_length_1118_cov_1.453434, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000587 DAOQVW010000000
VERSION     DAOQVW010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190765_length_1118_cov_1.453434"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="npdG"
                     /locus_tag="PKK85_09795"
     CDS             <1..648
                     /gene="npdG"
                     /locus_tag="PKK85_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNS26347.1"
                     /translation="GTGDQCLGLALRFAKAGEEVIIGSRDVKKALNAVNLVKKMIREE
                     INNIQAATNSEAAQEGDIVLLTVPLQAQMATLSSIKDDVEGKIIVDATVPLESCIGGS
                     PVRFVNVWEGSAVERTAEFLKDKNVQVISAFNNISAASLTNIDSEVDCDCLISGEDEE
                     AKTLVMELAEKIPGVRSIDCGGLENARIVEKITPLLINLNIRNRIKLAGIRITGL"
     gene            812..>1118
                     /locus_tag="PKK85_09800"
     CDS             812..>1118
                     /locus_tag="PKK85_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNS26348.1"
                     /translation="MIETIINSDATFIVGSGRSILIGKAFAMHLMHLGFTVYVVGDVT
                     TPALTDKDCLIAISGSGETRTVTLAAETAKEVGTKVVGVTTDLDSTLGKNADVVVNID
                     "
BASE COUNT          378 a          226 c          262 g          252 t
ORIGIN      
        1 gggactggag atcaatgctt gggattagca ttaagatttg caaaggcagg tgaagaggta
       61 ataattggct ctagagatgt taaaaaagca ttaaatgcgg tgaacttggt taaaaaaatg
      121 atcagggagg agataaacaa cattcaagca gccaccaata gtgaagcggc tcaagaagga
      181 gatatcgttc ttctaaccgt gcctttacag gcccagatgg caaccctcag cagcataaag
      241 gatgatgtag aagggaaaat aatagtagat gcaacagtcc ccctggaaag ctgtataggc
      301 ggttccccag tccgattcgt taacgtatgg gaaggttctg ccgtggaaag aactgcagaa
      361 ttcctgaagg acaaaaatgt ccaggtgata tccgccttca acaacatcag cgcagcctca
      421 ctcaccaaca tagattcaga agtggactgt gactgcctga tatccggaga agatgaagaa
      481 gcaaagacac tggtaatgga acttgccgaa aagatacccg gtgtcagatc catcgactgt
      541 ggaggtctgg aaaacgcccg tatagtggaa aagatcacac ccttattgat aaacctgaac
      601 atcagaaaca ggataaaact ggcgggaata aggatcaccg gtctttaatc ggaacatctt
      661 taaccataac cagataattc tattattata atctaaaatt taaaactttg aggcggttaa
      721 aacatatgga gtacataaaa gaacagccca ggaaataacc aaacacgccc tggcagttat
      781 aggccagata gacaaggtcc agaaggataa gatgatagaa accatcataa attctgacgc
      841 caccttcatc gtgggcagtg gaaggtcaat actcattgga aaagcatttg ccatgcacct
      901 gatgcacctt ggcttcaccg tgtacgtggt gggagatgta accactcccg ctctcactga
      961 taaagactgt ttaatagcca tttctggatc tggagaaaca agaaccgtta ctcttgccgc
     1021 tgaaactgct aaagaagttg gaaccaaggt tgtaggcgta actacagatc ttgattccac
     1081 ccttggaaaa aatgctgatg ttgtggtaaa cattgaca
//
LOCUS       DAOQVW010000588         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_191782_length_1114_cov_2.740321, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000588 DAOQVW010000000
VERSION     DAOQVW010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191782_length_1114_cov_2.740321"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="PKK85_09805"
     CDS             complement(<1..135)
                     /locus_tag="PKK85_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNS26349.1"
                     /translation="MRAVAKAPGKIILFGEHAVVFGKPALAMAVNCQARVEIRRVEEE
                     H"
     gene            complement(150..995)
                     /gene="amrB"
                     /locus_tag="PKK85_09810"
     CDS             complement(150..995)
                     /gene="amrB"
                     /locus_tag="PKK85_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNS26350.1"
                     /translation="MSHTRKAAWAGAFYEASPRDLKRRIRWSYQHRLGPGTLPEARGQ
                     ARSIKGLVAPHAGYIYSGPVAAHSYLELARDGFPETFIILSPNHTGLGSLVSTMTTGA
                     WETPLGVVEIDSELARELNHSPIIDDDPTAHLKEHSIEVQLPFLQYLEEDFQMVPLTM
                     MMQDLETSQEVGEAIHQAVKATGRDVVVLASTDLTHYKAQEVAAMEDQKVLQALEKLD
                     EVAMQEVVEKYNVNMCGYGPVAATLTATKLMGATQAQVLKYATSGETSGNYQEVVGYA
                     AAVIR"
BASE COUNT          181 a          334 c          325 g          274 t
ORIGIN      
        1 gtgttcctct tccacccttc tgatctccac ccgggcctgg cagttgacag ccatggccag
       61 ggctggtttg ccaaagacca cggcgtgttc tccaaaaaga atgatcttgc ctggtgcttt
      121 tgccacggct ctcatgctca atattcccct tagcgtatca ccgccgcggc gtatcccacc
      181 acttcctggt agttgccgct ggtctctcca ctggtggcgt atttaagcac ctgggcctgg
      241 gttgctccca ttaacttggt ggcggttagg gtggcggcca ctggtccata gccacacatg
      301 ttcacgttgt acttctccac cacttcctgc atggccacct catccagttt ctccagggcc
      361 tggagtactt tttggtcttc catggcagcc acctcctggg ctttgtagtg ggtgaggtcg
      421 gtgctggcca gtaccaccac atccctccct gtggccttta cagcctggtg gattgcttcc
      481 cccacctcct ggctggtctc cagatcctgc atcatcatgg tgaggggcac catctggaag
      541 tcctcctcca ggtactggag gaaggggagc tgcacctcga tgctgtgctc tttcaggtgg
      601 gcggtggggt cgtcgtcgat gatggggctg tggttgagtt cacgggccag ttcagagtct
      661 atctccacca cccccagggg cgtttcccag gccccggtgg tcatggtgga caccaggctg
      721 ccgaggccag tgtggttggg acttaggatt atgaatgtct cggggaatcc gtccctggcc
      781 agttccaggt aactgtgggc tgccactggt ccggagtaga tgtaacctgc gtggggggcc
      841 accagtccct tgatggatcg ggcctggccc ctggcttcag gtagggtgcc tgggcccagc
      901 ctgtgctggt aggaccagcg gatcctccgc ttcaagtcac ggggactggc ctcgtagaag
      961 gcaccggccc atgctgcctt acgggtgtgg gacaaatata aaaccccctg tactttagat
     1021 cttgagttcg aattcggtgg gtgctacatc caactcctcg tcctcagcta agactccctt
     1081 ggaacggagc atctgcaggg ccaggagcca gtac
//
LOCUS       DAOQVW010000589         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_192131_length_1113_cov_2.396975, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000589 DAOQVW010000000
VERSION     DAOQVW010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192131_length_1113_cov_2.396975"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(640..>1113)
                     /locus_tag="PKK85_09815"
     CDS             complement(640..>1113)
                     /locus_tag="PKK85_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNS26351.1"
                     /translation="VIMAITAPMAGRMSDRFNAHKIAALGMATVTLALFTFIFLDANT
                     PTNSIILGLAILGLGFGLFSSPNTNVIMGSVERRFYGVASATVSTMRLIGQTMSIGIA
                     TLVFALIIGRVQITPAQFPALLESIHLCFIVFTILCFMGIFASLKKDGEMKEEKN"
BASE COUNT          327 a          217 c          219 g          350 t
ORIGIN      
        1 ttttaacata ttggttttca ttaggatgag atgtctgcag tgatccatct ttgatgatta
       61 tatctccttc ttcaagttct gaatcgatta tgtgctctgc gatagtccat tctgagaatc
      121 ttctagccat agaagccatt ctttccatca ttaactgatt tcctaataca aaatcgcgtt
      181 caaatgcctt gatcttcaga tccttttcca ggggaagata ttttctaaaa aaatcattct
      241 ctagagaaaa tttagtttca aaaaaaaggt taccatggtc aaaattagat gaagtgtacg
      301 ataaaaactc tattctacac ggaatattgg atttaggaga aatgagtttc ttattttgga
      361 aaacattaaa ataaacccgg ttcaattgca ctgaatagcc tggagtgggt aataattcct
      421 ggtttccacc atcaatacag gaaatttttc tatcagattt tgatacctca attttatgga
      481 aattttcctc ttctaatgaa tttaaatcat tatcttcttc attaaaaata tcacctaggt
      541 cacgatagga aatatcagaa ctaagaattt cagcaatttc tcttatactt ttaatgtaat
      601 cacccctact gtaaatattt actatttaca aaagggtatt taattctttt cttctttcat
      661 ctccccatcc ttcttcagtg atgcgaagat acccatgaag cacagtatgg tgaagactat
      721 gaagcacaag tgtatgctct ctagaagggc agggaactga gctggggtta tctgcactcg
      781 gccgattatg agtgcgaata ccagggtagc aatcccgata ctcatggtct ggccgatgag
      841 tcgcatggta ctaaccgtgg ccgaggctac tccgtagaat cttcgctcca ctgatcccat
      901 tataacgttg gtgttgggag atgagaatag gccgaatccc aagcccagta ttgctaatcc
      961 aagtatgatg ctgttggtag gggtgttagc atccaagaat atgaaggtga aaagtgccaa
     1021 agttacggtg gccataccga gggcggctat cttatgggcg ttaaagcggt ccgacattct
     1081 ccctgccatg ggggctgtga tggccataat aac
//
LOCUS       DAOQVW010000590         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_192134_length_1113_cov_2.387524, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000590 DAOQVW010000000
VERSION     DAOQVW010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192134_length_1113_cov_2.387524"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKK85_09820"
     CDS             <1..201
                     /locus_tag="PKK85_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS26352.1"
                     /translation="LGPVIKTKKLLIDQEACVQCQICEENCPMEAIELEGDKVVLSED
                     KCILCNVCSTKCPVGALKLEGT"
     gene            202..837
                     /locus_tag="PKK85_09825"
     CDS             202..837
                     /locus_tag="PKK85_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS26353.1"
                     /translation="MKVKEIMDEEFIYITQDQSIEEVSIIMENGKKFTTPVVDENMHL
                     VGWITSFELTKGLREGNEKISEVMHSKDSIVHVHENNPARLAVLETSHNKFVSIPVLN
                     DDGVVVGVVRSFDIVDTLSKLYEIKVYKIFKAMEQELKGVSWDELMEASAIVTRRRTG
                     ENVTAEEYEFRIKEATFGEAIWATGGLEKFFVGLIAIGQLVTAKKVARARK"
BASE COUNT          373 a          167 c          230 g          343 t
ORIGIN      
        1 ttagggccgg ttattaaaac caaaaaactc ctaatagatc aggaagcatg tgtccagtgt
       61 cagatttgtg aagaaaactg tcccatggag gcaattgaat tagaagggga taaagtagtt
      121 ctttcagagg ataaatgtat attatgtaat gtctgttcaa ctaaatgtcc agttggagca
      181 ttgaaactgg aggggaccta attgaaagtg aaagagataa tggatgaaga atttatttac
      241 ataacccagg atcaaagtat tgaagaagta tcaataatca tggaaaacgg gaaaaaattc
      301 accacaccag tggtggatga aaatatgcac ctggtagggt ggatcacatc ctttgaattg
      361 actaaaggat tacgagaagg aaatgagaaa ataagcgaag ttatgcattc taaggacagc
      421 atcgtccacg tccatgaaaa taatcccgcc cgactggcgg ttctggagac atcccataat
      481 aaattcgtga gcatcccggt tttaaatgat gatggagtgg tggtaggggt cgtacggtcc
      541 ttcgatatag tggataccct ttctaaactc tacgaaatta aagtgtataa gatatttaag
      601 gctatggaac aggaacttaa gggagtaagc tgggatgaac tcatggaggc atcagccatc
      661 gtcacccgca gaagaactgg agaaaatgtt accgcagaag aatatgaatt ccgtatcaaa
      721 gaggcaacct ttggagaggc aatctgggca actggtggcc tggaaaaatt tttcgtggga
      781 ctgattgcaa taggacaatt agtaaccgcc aagaaagttg ccagggctag aaaatagaat
      841 tctggcctta atttatttaa tttttaattt tccgtgattt aatttatttc attttttatt
      901 aatttttatt taatttatta tttcctctat tttctattta atttatttta tttttcaatt
      961 tccccgtcat tcaaggtatt tatttgaaca tcacaattgt tatatttata tggtaagtgg
     1021 tctaatttta taaataccat caattttcta tttctatatt tttagaaaaa tttaggagtt
     1081 ttaaccatga gattcaatac aaaatcagta cat
//
LOCUS       DAOQVW010000591         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_192285_length_1113_cov_1.619093, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000591 DAOQVW010000000
VERSION     DAOQVW010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192285_length_1113_cov_1.619093"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PKK85_09830"
     CDS             complement(<1..493)
                     /locus_tag="PKK85_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNS26354.1"
                     /translation="MPILLFGSDHIDLITGKKTTTIRKLWKKPLKVGDTLHCYWNHVS
                     KERRKLFEAEITDVEVIDYIHLKNNDELAREEGYSDAKELRKEFRRLYPDVDDDTKFQ
                     IIRFRKLPIEEWEGQKIDEKAMVTKRADILFDVGKFEDSVLCYTAALRFDPDDIYLLN
                     RKGD"
     gene            complement(569..>1113)
                     /locus_tag="PKK85_09835"
     CDS             complement(569..>1113)
                     /locus_tag="PKK85_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS26355.1"
                     /translation="IQDSLNFIDSLVITGGEPLVQFEDLKELLNYLKDTELKIKLDTN
                     GCYPERLEEIIHLVDYVALDVKAPANKYEEITGASIWDDVKKSMEIVRKSPNTFLECR
                     TTYVPGLLDKGDIQEIVKDTKCDLYTIQQFRNRVVLDEKLKNTPSPSRDELLEIAREI
                     KSSIPRVKIKTGEFGEEIIE"
BASE COUNT          264 a          234 c          223 g          392 t
ORIGIN      
        1 tgtctccctt acggtttaag aggtaaatat cgtcagggtc aaaacggagt gctgctgtat
       61 aacacagcac agaatcttca aatttaccca catcaaaaag aatatccgcc ctcttggtta
      121 ccatggcctt ttcatcaatt ttttgacctt cccactcttc tatgggtagt ttgcggaatc
      181 ttattatctg gaacttggta tcatcatcta catcagggta taatctgcgg aattcttttc
      241 ggagttcctt agcatcactg taaccttctt ctcgtgccag ttcatcgttg ttctttagat
      301 ggatgtaatc tattacctca acatcggtaa tttccgcctc aaaaagcttc cggcgctcct
      361 tggagacatg gttccagtaa cagtggagtg tgtcacctac tttaaggggt ttcttccata
      421 atttcctgat ggtggtggtt tttttaccgg ttataaggtc tatgtggtcg cttccaaata
      481 gtaggattgg tatgggggga acctcctgta aaactttttt tgggtaaact tttttgattt
      541 ctggatagaa caggtgatat taaatttatt attcgattat ttcctctcca aattcgccgg
      601 tttttatttt aacccggggt atagatgatt tgatctccct tgcaatctcc aggagttcat
      661 cgcgggatgg actgggagtg ttctttagtt tttcatctaa aacaacccgg ttcctgaact
      721 gttgaatggt atatagatca catttagtat ctttgacaat ttcctggata tcccctttat
      781 ctaaaagacc gggcacgtag gtggttctgc actctaggaa agtgtttgga gactttcgga
      841 ctatctccat actcttttta acatcatccc atattgaggc acctgttatc tcttcatact
      901 tattagctgg ggctttaaca tccagtgcaa cgtagtctac caggtggatt atttcctcca
      961 gcctctctgg gtaacagcca ttggtatcca gtttgatttt taactcagta tctttcaggt
     1021 agtttaagag ttccttaaga tcttcaaact ggactaatgg ttctcctcca gttataacca
     1081 ggctgtctat gaagttcagg gagtcctgga ttt
//
LOCUS       DAOQVW010000592         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_192579_length_1112_cov_1.650899, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000592 DAOQVW010000000
VERSION     DAOQVW010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192579_length_1112_cov_1.650899"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKK85_09840"
     CDS             <1..206
                     /locus_tag="PKK85_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26356.1"
                     /translation="LGEIYKNYIYNPETDEHDERYLVEVSTTIDERISDGFYYTSAMN
                     KFKEILNNPEELEEKLTNYPVDQ"
     gene            232..>1112
                     /locus_tag="PKK85_09845"
     CDS             232..>1112
                     /locus_tag="PKK85_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNS26357.1"
                     /translation="MNISSLIVEAEDRTNYKRPWLKHYNRETPANLTYPDKTIYQMLQ
                     ARTEKDPGNMALEFMGKKTRFKTLLEKVDNCARALKEIGVTEKDAVTICMPNTPHAVI
                     MFYAVNRVGAIANMIHPLSAPKEIEFYMNNVDSKYIIIGNFSYPNLKKVEGDLKLKKV
                     IVAKVSDFLGKRNRVGFWALGGRKIPKVNYNDNSLLVSWKNFIKLGENSSHDVKSSMK
                     CKDRAVVLYTGGTTGIQKGVLLSNHNFNALVIQCLAHMNDGYLGKIKEDKMMCILPMF
                     HGFGLAVCVHLPISVGA"
BASE COUNT          357 a          252 c          263 g          240 t
ORIGIN      
        1 gcctggggga aatctataaa aactatattt acaacccgga aacggatgaa cacgatgaaa
       61 ggtacctggt cgaagtttcc accaccatcg atgaacgtat cagtgacgga ttctactaca
      121 ccagcgccat gaacaaattc aaggaaatcc tgaacaaccc agaagagctg gaagaaaaac
      181 taaccaatta ccctgtggat caataaaaac atcaatgaat aggttaccga tatgaacatc
      241 tcaagcttaa ttgtagaagc tgaagacagg acaaactaca aacggccctg gcttaagcat
      301 tacaacaggg aaacaccagc aaatctcacc tacccagata agactatcta ccaaatgtta
      361 caggccagga cggagaaaga tccaggtaac atggccctgg aattcatggg gaagaagacc
      421 cggttcaaaa cgctcctgga gaaggtggac aactgcgccc gggcactcaa ggaaattggt
      481 gtaacagaaa aggacgccgt taccatctgc atgcccaaca caccccacgc ggtgatcatg
      541 ttctacgctg taaacagggt aggggccatc gccaacatga tccatcccct gagcgctcct
      601 aaagagatcg agttctacat gaataacgtg gacagtaagt acatcataat cggcaacttc
      661 tcctatccca acctgaagaa ggtggaaggg gatttaaagc ttaaaaaggt gatagtggcc
      721 aaggtaagcg attttctggg taaacgtaat agagtaggct tctgggcttt aggaggccgt
      781 aagataccta aagttaatta caatgataac agtttactgg tttcctggaa aaatttcatc
      841 aaattgggag aaaattccag ccatgatgtg aaatcttcca tgaagtgcaa ggaccgagct
      901 gtggtattat acaccggcgg aaccacgggg atacagaagg gtgttttact ctcgaatcac
      961 aacttcaacg ccctggtcat ccagtgtctg gcgcatatga acgatgggta tctgggaaag
     1021 attaaggagg ataagatgat gtgcatcctg cccatgttcc atggcttcgg cctggccgtc
     1081 tgtgtccatc tccctatcag tgtaggtgcc ag
//
LOCUS       DAOQVW010000593         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_193049_length_1110_cov_2.295735, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000593 DAOQVW010000000
VERSION     DAOQVW010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193049_length_1110_cov_2.295735"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKK85_09850"
     CDS             <1..652
                     /locus_tag="PKK85_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26358.1"
                     /translation="IYQFFSCLSVLIILSSLFLLLRPFKKFTGKGDRRIIIFSLITLL
                     IYSGGYLPVLVEDRYLWPMYLLLMLMGGLLISLTFKNLPAWKTNYVNILKGVIIILFA
                     LSFILMPVNFLNTNIDTGKDIYALSDTLKTQYGVQGNIATNDRLIDAQFLSFYLNTTS
                     FGQSKRDISDAELQLQLEKYRIDYYLVWGNSTPHLTSYKEITGGKIRDLKIYRRNL"
     gene            complement(694..>1110)
                     /locus_tag="PKK85_09855"
     CDS             complement(694..>1110)
                     /locus_tag="PKK85_09855"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HNS26359.1"
                     /translation="GGPSIERAGNSPQYIKELDLPILGICLGHQLIAQVYGGEIGSAG
                     IESYAKNTITILDENDIFQGLGKTMDVWTSHKDEVRTLPPNFKLLATSSLCDIEAMKH
                     KDKPIYGIQFHPEVHHTKQGHLIFENFYRVCQEFKK"
BASE COUNT          306 a          203 c          230 g          371 t
ORIGIN      
        1 catctaccaa ttcttctcct gtttatcggt attaatcatt ttaagtagtt tgttcctctt
       61 attacgtcct tttaagaagt ttacaggtaa gggtgatagg agaattatca tattttccct
      121 gataactctc ctaatatact ccggaggata cctgcctgtt ttagtagagg acagatactt
      181 atggcctatg tatttactcc tgatgttgat gggtggcctt cttataagtt taacctttaa
      241 gaacctgcca gcttggaaaa ctaactacgt aaatatttta aaaggagtta taattatctt
      301 atttgcatta tccttcatct taatgcctgt aaattttctg aatactaata tagatacagg
      361 aaaagatata tatgctttga gcgatacctt aaaaacccag tacggggtgc agggcaacat
      421 agccaccaac gataggttaa tagacgctca atttttatcc ttctacctta acaccacttc
      481 cttcggccag tcaaagaggg atatttccga tgcagagctc cagctacagt tggagaaata
      541 tagaatcgat tactacttgg tatggggcaa ttcaacaccc catttaacta gctacaagga
      601 gataactggg ggtaaaataa gagatttgaa gatttataga aggaatttgt agtattataa
      661 ggggtttgga gttattaaaa aattagaact gacttatttt ttaaattcct gacagacacg
      721 gtagaagttt tcaaatatca ggtggccctg tttggtgtgg tgtacttccg ggtggaactg
      781 gattccatag atgggtttat ccttatgttt catggcttct atatcgcaca gggatgatgt
      841 agccagtaat ttgaaatttg ggggtaaagt tctgacttcg tctttatggg aggtccatac
      901 atccatggtt ttccccaggc cctggaatat gtcgttttca tccaggatgg tgatggtgtt
      961 tttggcgtaa ctttctattc ctgcgcttcc tatttctcca ccataaacct gggctataag
     1021 ctggtggccc agacagatgc ctaatatggg gagatctaac tctttaatgt attgaggtga
     1081 gtttccagct ctttctatag agggtcctcc
//
LOCUS       DAOQVW010000594         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_193557_length_1108_cov_2.615385, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000594 DAOQVW010000000
VERSION     DAOQVW010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193557_length_1108_cov_2.615385"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKK85_09860"
     CDS             complement(<1..809)
                     /locus_tag="PKK85_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNS26360.1"
                     /translation="MTCNILVGGSWGDEGKGKCITYLCYKDQPDIIARAGVGPNAGHS
                     VEFHGDKYGLRMIPSGFVYTGARLLIGAGVLVDPEVFHYELDYLNKYQVKGRTFADIR
                     CSIIEPKHKEQDKASEYLAKKIGSTGTGCGPGNRDRVMRVAKQARDWESMEGFTADVP
                     LEVNTALDEGRDVFIEGSQGYGLSLYYGTYPFVTSKDTTASTAAADIGVGPTRIDEVI
                     VVFKSYTTRVGEGPFPTEIKQDEAEKRGLEEYGTVTGRRRRVGEFDMDFAR"
BASE COUNT          218 a          310 c          292 g          288 t
ORIGIN      
        1 tcccgggcga agtccatgtc aaactcaccc acccggcgtc tgcgtcccgt gactgtgccg
       61 tattcctcca gtccccgttt ctcggcttcg tcctgtttta tctcggtggg gaagggtccc
      121 tctcccaccc gggtggtgta ggatttgaag accactataa cctcatcgat gcgggtgggg
      181 cccactccga tgtccgcagc ggcggtggag gcagtggtgt ccttgctggt tacgaagggg
      241 taggtgccgt agtagaggga gaggccatag ccctggctgc cctctatgaa tacatcccta
      301 ccctcatcca gggctgtgtt tacctccagg ggcacgtcgg cggtgaagcc ctccatactc
      361 tcccagtccc tggcctgctt ggccacccgc ataacccggt cccggtttcc tggtccgcag
      421 cctgtgccgg tgctgccaat cttcttggcc aggtactcgg aggccttatc ctgctccttg
      481 tgtttgggct cgatgatgct gcaccttata tccgcgaagg ttcgtccctt cacctggtac
      541 ttgttcaggt agtccagttc gtagtggaac acctcggggt ccaccagcac ccctgctcct
      601 atgaggaggc gggctccggt gtagacgaat cccgagggta tcatgcgcag gccatacttg
      661 tccccatgaa attccacgga atggccagcg ttagggccca caccggctcg ggcgatgatg
      721 tctggttggt ctttgtagca caggtaggtg atgcattttc ctttgccttc atctccccag
      781 ctgccaccaa ctaatatgtt acaagtcatg gtatcactca atgttttact agaaacttca
      841 aacctctcat atattggaac acccctcata aaagattttc catatcacaa tggggaaatg
      901 gtgttccggt agttaagagt catggttttt tttagaataa agcctccacg gggcatagga
      961 caggggggat aagttgggtt tgatcccaac tcctaaatgc agggtattaa cccatttcgc
     1021 ccccatacag gtctctaaca cggagaagtg aatttgatca tcatccatca ctggagtgtg
     1081 tataatttag gggatggtgt gattagcc
//
LOCUS       DAOQVW010000595         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_194359_length_1106_cov_1.587060, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000595 DAOQVW010000000
VERSION     DAOQVW010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194359_length_1106_cov_1.587060"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1106
                     /locus_tag="PKK85_09865"
     CDS             <1..>1106
                     /locus_tag="PKK85_09865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019263303.1"
                     /note="unique DNA polymerase that is similar to eukaryotic
                     polymerases; forms a heterodimer of the small and large
                     subunits; possesses polymerization and 3' to 5'
                     exonuclease activities; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HNS26361.1"
                     /translation="PKYTYFYHDASAQEINRVRSWLNENINDNKIIADISPDKRILEI
                     LGVEHRVEDAKVIIDADDTYALLNTLPEPLDEELKTMEAINKVSPIQIMAKAPTYLGG
                     RVGRPEKTKERMMKPAPHALFPIGNAGGSRRNIVDAAKKGNITVEIARCRCTQCGIGS
                     MQAICPACGGRALPTGSGRKKINLGHLLKKAYERVGIRKMDEIKGVIGMISEDKFPEP
                     LEKGILRAKNGVYTFKDATIRHDSTNLPLTHFIPREIKVTHQKLRELGYTHDIKGDEL
                     ESDEQTVEIKVQDVVISDNCGEYLLKVANFVDDLLDKFYGMDRFYNLKSYKDLVGHLV
                     VGLAPHTSAGVLGRIVGFTKASGCYAHPYFHSAK"
BASE COUNT          352 a          252 c          267 g          235 t
ORIGIN      
        1 atcccaaata tacttacttt tatcatgatg cctctgccca ggaaatcaac cgtgttcgaa
       61 gctggttaaa tgaaaatatc aacgataata aaattatcgc tgatataagt ccggataaga
      121 ggatattaga gattttaggt gttgaacaca gggtagaaga cgcgaaagtc atcattgatg
      181 cagacgacac ctacgcccta ttaaatacct taccagaacc actggatgaa gaactaaaaa
      241 ctatggaagc aattaacaag gtttcaccaa tccagataat ggccaaagca cctacttacc
      301 tcggtggtag ggtgggcagg ccagagaaaa ccaaggaaag gatgatgaaa cccgcacccc
      361 atgcactgtt ccccataggc aacgcaggag gtagcaggcg taacatagtc gatgcagcga
      421 aaaaaggaaa cataacagtt gaaatagccc ggtgccgctg cacccaatgt ggtattgggt
      481 ccatgcaggc catctgcccc gcctgcggag gaagagccct gcccaccggt tccggtagga
      541 agaagataaa cctgggccac ctcctaaaga aagcctatga aagggtgggc atccggaaga
      601 tggatgaaat caaaggagta atcggaatga tatccgagga taaattccca gaacccctgg
      661 agaagggtat tctacgggct aaaaatggtg tttacacctt taaagatgca actatccgac
      721 atgactccac caatctaccg cttacccact tcataccccg tgaaatcaaa gtcacacacc
      781 agaaattaag ggaactggga tacacccatg atatcaaagg ggatgaactg gaaagtgacg
      841 agcagacagt ggagataaaa gtacaggacg tggttatctc tgataactgc ggggaatacc
      901 tcctgaaagt agccaatttc gtggatgact tactggataa gttctatggc atggacagat
      961 tctacaatct gaagagttac aaggatctgg tgggacacct ggtggtgggc ctggcacccc
     1021 atacatctgc cggtgttctg ggccggatag ttggttttac taaagcctct ggatgttacg
     1081 cccaccccta cttccacagt gctaaa
//
LOCUS       DAOQVW010000596         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_194484_length_1105_cov_2.664762, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000596 DAOQVW010000000
VERSION     DAOQVW010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194484_length_1105_cov_2.664762"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PKK85_09870"
     CDS             complement(<1..1094)
                     /locus_tag="PKK85_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNS26362.1"
                     /translation="MGQSNTPYKLAIVNYDETVTIPMTGERINFGNNLTQLLEEAKYE
                     NSDVNLFNVTKMSESEADALLKTRDIDAALIIPDNFSESVLALINHTIMTTTNPLAAS
                     QSTSPDITSTLIIRGDTGFINFGVSQGILVGVLAQYQDEIVIQTQNAVQGTPGAQPTV
                     FLNSKVEPIEGTQNFTSFDFIAPGMIVFAILLLATTVAANFTREVESGTLARLKLSKM
                     KGFDLMFGGLIPWSLVVVVQVIILLVVAVAIGFHWQGGLNSILLAILVGVIGGIASIA
                     LGMIIAAFVTSVSQATNLGTLITVPASFLVGAFFPLPDVEIFGIPFNAFLPWTHILDA
                     LRSTLTYGGGWNDISYSVAVSLVLTVILFA"
BASE COUNT          298 a          264 c          271 g          272 t
ORIGIN      
        1 atggcaaaga gtatcaccgt cagaaccaaa ctaacagcta ctgagtagga tatatcgttc
       61 catcctccac cataggttag tgtagagcgg agggcgtcca gtatatgggt ccagggtaag
      121 aaggcgttga atgggatgcc gaatatctcc acgtcaggaa gtgggaaaaa ggctcctacc
      181 agaaaactcg ccgggacagt gatcagggta cccaaattag tcgcctggct cacactggta
      241 acaaatgctg ctattatcat tcccagtgca atagaggcta tacccccaat tacacccact
      301 aagatggcca gaagtatgga attaagaccg ccctgccagt gaaatcctat ggccactgcc
      361 actactagga gtatgatcac ctggacaacc accaccaggg accagggtat aaggccgccg
      421 aacatgaggt cgaatccctt catcttggat aacttcagcc gggccagggt cccagactcc
      481 acctcccggg tgaagttggc agccaccgtg gtagctagga ggagtatggc aaatacgatc
      541 atcccaggtg caatgaaatc gaaactggtg aagttctggg tcccctcgat gggctccacc
      601 ttggaattta aaaatacagt cggctgagca cccggggttc cctggactgc gttttgagtc
      661 tgaattacta tttcatcctg atactgggcc agcacaccaa ctaaaatgcc ctgggatact
      721 ccaaaattaa taaatccagt gtcaccacgg attattaggg tggatgttat atctggtgag
      781 gttgattgac ttgctgccaa tggattggtg gtggtcatga tagtatggtt tatgagcgcc
      841 aataccgact ctgaaaagtt atccggtata atcaacgcgg catcgatgtc cctggtcttt
      901 aaaagagcgt cagcctcact ttcactcatc ttggtcacgt tgaagaggtt aacgtcacta
      961 ttttcatatt tagcctcttc taaaagttga gtaaggttat ttccaaaatt gatgcgttca
     1021 ccagtcatag ggatggtgac tgtctcatca taatttacaa tggccagttt atagggggtg
     1081 ttgctctgcc ccatgccccc gaagg
//
LOCUS       DAOQVW010000597         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_195793_length_1101_cov_2.158700, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000597 DAOQVW010000000
VERSION     DAOQVW010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195793_length_1101_cov_2.158700"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1074
                     /locus_tag="PKK85_09875"
     CDS             97..1074
                     /locus_tag="PKK85_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNS26363.1"
                     /translation="MNFKYLKELKDCHLCPWDCGINRLDGGKGVCRVGMPEVAYTNFT
                     EVLKSYSITLLGCSFFCTYCNAYRISQYPESGWRYRGYVDPPALVEEILHAFKSQYAQ
                     DMSVNKLSFTGGEPSIHTPYLEAVVGLLNKEISGLEKQESPPLEKQGISQMESLESPH
                     QLDVGLATNGFITRKTMERLSRISSYFNFEIKAYDTELHQALTGAPSEPVLENAEWLA
                     KKHPEKIRVFRTVVIPGLNQEEIHKIARFIQEIDPSIHYRLVGFRPHFMLYYHPAPTR
                     SLMQELVEDCKKLGLKNVDYSGYHPGGSQMTEQMSNNGLKTLYHHLNRE"
BASE COUNT          327 a          255 c          241 g          278 t
ORIGIN      
        1 tttctcaggc ccgtttaggc gcttctgtgc ctgtatatta tttatattaa aaaatataga
       61 ttaatcctat aaaaatttaa ccaatgatgg gagaacatga actttaaata cctgaaggaa
      121 ctgaaagatt gccatctatg cccttgggac tgtggaataa accgtttaga tggaggaaaa
      181 ggggtttgcc gtgttggaat gccagaagtg gcctatacca atttcacaga ggttttaaag
      241 agttactcca tcaccctgct gggctgttcc ttcttctgca cttactgtaa tgcctaccgt
      301 atctcccagt atcctgaaag cggttggagg taccggggat acgtggatcc ccctgcttta
      361 gtcgaagaaa tcctccatgc atttaaaagc cagtatgccc aggatatgtc cgtaaacaag
      421 ttgagtttca ccggtggaga accatcaatc catacaccct atctggaggc ggtagtggga
      481 cttctaaata aggaaatttc tggtctggaa aaacaagaaa gtcccccact ggaaaaacaa
      541 gggatttccc aaatggaaag cctggagagt ccccaccagc tggatgtggg cctggccacc
      601 aacggattca tcaccaggaa aaccatggaa aggctctcta gaatttcatc ctatttcaac
      661 ttcgaaataa aggcctacga taccgaactg catcaggccc ttaccggagc accttcagag
      721 cctgtcctgg agaatgccga gtggttagcc aagaaacatc cagaaaaaat ccgggtattc
      781 cgtaccgtgg ttatccccgg gttaaatcag gaagaaatcc ataaaatcgc cagatttatc
      841 caggagatcg acccatccat ccattaccgg ctggtgggtt tcaggccaca cttcatgctc
      901 tactatcacc ccgcccctac gcgctccctg atgcaagagt tagtggaaga ttgtaaaaaa
      961 ctgggcctta aaaacgttga ttattctggt tatcaccctg gtggaagtca gatgaccgaa
     1021 caaatgagta acaatggctt aaaaaccctt tatcatcatt taaaccggga ataacaagat
     1081 tggtaatttc aaggtgttta c
//
LOCUS       DAOQVW010000598         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_195890_length_1101_cov_1.613767, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000598 DAOQVW010000000
VERSION     DAOQVW010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195890_length_1101_cov_1.613767"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /gene="hypD"
                     /locus_tag="PKK85_09880"
     CDS             <1..882
                     /gene="hypD"
                     /locus_tag="PKK85_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNS26364.1"
                     /translation="CVPSREVDEIMYLAREGVTVTTFGDMLRVPGTNGCLADARAEGA
                     DIRIVYGVGNAVEIAKKLDNEVVFMAAGFETTAPTTASEIVSGPPENFSLLSCHRLIP
                     PALKFLIESGEVNLNALIEPGHVSTIIGTQPYEVFSEKYGIPQVIAGFNPFDVLIAIY
                     LILKQIKEENPHVQNEYKRAVRTEGNVKAQELMDEVFYIKSKEWRGFPSIPDSVYEIR
                     DTYSQFNAREKFDIPELESKEVVTGCICGPILRGVARPEECKLFRKECNPVNPVGACM
                     VSKEGTCNIAYRYGSLK"
     gene            937..>1101
                     /locus_tag="PKK85_09885"
     CDS             937..>1101
                     /locus_tag="PKK85_09885"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNS26365.1"
                     /translation="MEEHELKAIALDVDGTMTDNSRKICISAIEAISQAEENGIPVII
                     VTGNILCAAKM"
BASE COUNT          340 a          251 c          259 g          251 t
ORIGIN      
        1 tgcgtaccct ccagggaagt ggatgaaatc atgtacctgg caagagaggg agttactgta
       61 accacgtttg gtgacatgct ccgggtgcct ggaacaaacg gctgcctggc cgacgcccgg
      121 gcagagggag cagatataag gatagtctac ggggttggca acgccgttga aattgcaaag
      181 aaactggaca acgaagtggt tttcatggct gccgggtttg agaccaccgc acctaccact
      241 gcctcggaaa tagtctccgg accaccggaa aacttctccc tactttcctg tcacagactc
      301 ataccacccg ccttaaaatt cctgatagaa tcgggtgaag taaacttaaa cgcccttata
      361 gagccgggac acgtatccac cataattggc acccaaccat acgaagtatt ctccgagaaa
      421 tatggaatac cacaggtaat agccggattt aatccattcg atgttctcat agccatctac
      481 ctcatactga aacagattaa agaagaaaac ccccatgtac agaatgaata taaacgcgca
      541 gttcgcacag aaggtaacgt taaggcacag gaactcatgg acgaagtatt ctacatcaaa
      601 agcaaggagt ggaggggatt ccccagcata cctgattcag tctacgagat ccgggatacc
      661 tattcccagt tcaatgcccg ggaaaaattc gacatacctg agctagaaag caaggaagtg
      721 gttactggat gtatatgcgg gcctatactg cggggggtgg cccgacctga agaatgtaaa
      781 ctattcagga aggaatgtaa cccggtcaat ccagttgggg cctgtatggt tagtaaagaa
      841 ggaacctgta acatagccta tcgttacggt tctttaaagt gattaatttt tcatctcccc
      901 caataatttt atatcccaaa cgaggtatca aattccatgg aagaacacga attaaaggcc
      961 atagctcttg atgtggatgg aactatgact gataattcca ggaaaatctg tataagtgct
     1021 atagaggcca taagtcaggc cgaagaaaat ggaattccag ttataattgt caccggcaat
     1081 atcctatgcg ctgccaaaat g
//
LOCUS       DAOQVW010000599         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_197209_length_1096_cov_3.294909, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000599 DAOQVW010000000
VERSION     DAOQVW010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197209_length_1096_cov_3.294909"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /gene="tuf"
                     /locus_tag="PKK85_09890"
     CDS             complement(<1..954)
                     /gene="tuf"
                     /locus_tag="PKK85_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HNS26366.1"
                     /translation="MAKEKEHMNLAFIGHVDHGKSTMVGHLLLQSGAIAEQQLSKGED
                     KFRFVMDQLTEERERGVTIDLAHARFDTPKYEFTIVDCPGHRDFVKNMITGASQADAA
                     VLVVAVDDGVMPQTKEHAFLARTLGINQLIIGINKMDLVNYDEEKFNALKEEVSDLIK
                     TVAYKPKEINFIPLSAFEGDNISKKSENTPWYKGPTLVQALDEFTAPEKPTDLPLRVP
                     IQDVYSITGVGTVPVGRVETGVMKKGENVIFEPPGVSGEVKSIEMHHEMLDLAEPGDN
                     VGFNVRGVGKNDIRRGDVAGHVTNPPTVAKEFTAQIVVLQHP"
BASE COUNT          231 a          263 c          271 g          331 t
ORIGIN      
        1 ggggtgctgc aggaccacga tctgagcggt gaattcttta gccaccgttg gtgggtttgt
       61 gacatgtcca gccacgtctc cacgacggat atcattttta ccgactccac gaacgttgaa
      121 cccaacgttg tctccaggtt ctgccaaatc caacatttca tggtgcattt caatggattt
      181 aacttctcca cttacacctg gtggttcgaa gatgacattt tctcccttct tcattacacc
      241 ggtttccacc cgtcccacag gcacagttcc cactccagtg atggagtaca catcctggat
      301 aggtaccctt aggggtaggt cggttggttt ttctggtgca gtgaattcat caagggcctg
      361 gaccagggtg ggtcccttgt accagggggt gttttcggat ttttttgata tattgtcccc
      421 ttcaaatgcg gagagcggta tgaagttgat ctccttgggc ttgtaggcca cagtcttgat
      481 gagatcggat acttcctctt tgagggcgtt gaatttttcc tcatcgtagt taaccaggtc
      541 catcttgttg ataccaatga tgagctggtt gatacccagg gtacgggcca aaaatgcgtg
      601 ttccttggtc tggggcatga ccccgtcgtc cacagccaca accagtacag cagcatcggc
      661 ctggctggcg ccggtgatca tgtttttcac aaagtcacgg tgacccgggc agtccacaat
      721 ggtgaattca tatttggggg tgtcgaatct ggcgtgagcc aggtcaatag ttacccctct
      781 ttccctttct tcagtcagtt gatccataac gaatcggaac ttgtcttctc ctttagatag
      841 ctgttgttca gcgatggctc cagattggag cagaaggtga ccgaccatgg tggatttacc
      901 gtggtctacg tgtccgataa acgccaagtt catgtgttct ttttctttag ccataagtta
      961 ttcctccata ttacagatta gtttaaagag catcttaagg tatttaggat aaccctatat
     1021 ttatgcttga tgtccactag cccaagtagt ggtcgggtcc gtatggttct ggtgagagac
     1081 ccttccggtt tctgat
//
LOCUS       DAOQVW010000600         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_197297_length_1096_cov_2.486071, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000600 DAOQVW010000000
VERSION     DAOQVW010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197297_length_1096_cov_2.486071"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..728)
                     /locus_tag="PKK85_09895"
     CDS             complement(150..728)
                     /locus_tag="PKK85_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26367.1"
                     /translation="MREKSLGLIILLVMVVLASGCTIQNPDEEKTNYYNSSSVFFNYP
                     DTWEIARSIEVAGVEGVIVTDPTAGKYINESSTLEELESLNITEGTAFIYITIPKTSE
                     LNLNSTVTDVQQRLAQKGAKVTRKRTTNVSGVVANETSLTRTSEGIISEARIIAFERN
                     DNIYTLILITAGNSYESQKKNFDLIFSSLQFT"
BASE COUNT          329 a          213 c          202 g          352 t
ORIGIN      
        1 ggatatagtg ctgcatgtgg ctctaccact ggtagcagtg atttgagtca tcaacaatta
       61 atttcatttt ttttaaaagt tttataaata aaatccagtg ccaaatttta tttgcataaa
      121 taagtcacaa gaaaagttat cagtggtttt caggtaaatt gcaggctact aaatatcaga
      181 tcaaagttct tcttctgact ttcatagcta ttaccagcag ttattagtat cagggtgtag
      241 atgttatcat tcctctcaaa agcaatgatc ctagcttctg agatgattcc ttcactagtt
      301 ctggttaaag aggtttcatt ggccaccacc ccggagacgt tggtggttct tttccttgtg
      361 actttagctc ccttctgggc cagtctttgc tgcacatcag tcaccgtgga attaagatta
      421 agctctgaag tttttgggat cgtaatatag ataaatgccg tgccctcggt aatatttaaa
      481 gattctaact cttccagtgt actggactcg ttaatgtact tacctgctgt tggatcagta
      541 actattaccc cttctacacc agcaacttcg atacttctgg caatctccca tgtatctgga
      601 tagttaaaga atactgaact ggaattatag taattggttt tttcttcatc cggattttga
      661 atggtgcaac cagatgccaa aaccaccatt accaatagta taatcagccc caaggacttc
      721 tccctcatcc aacactcccc tgtctttata gattaactta acacaagaag acaaatataa
      781 ttttagatta cagatagtaa taaaaatagc caagatagta gatcatcagc gaagtagtag
      841 taataattta cttcgttata ggttaatatt acgaagttaa tttttttccc agcacaatcg
      901 gtgcagaatt tttttttaga ggagtttgca tatgacacca gaaacatagt acaaggtata
      961 gaatggaact ctagatccat cgtgaattga ttttttttat ggtgaggacc atgatcaccg
     1021 ctggccagga cctgtactgc caccaaaaac caggagttac cggagccgta gtcaggtata
     1081 aggatagaaa ctgtgt
//
LOCUS       DAOQVW010000601         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_197448_length_1096_cov_1.663785, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000601 DAOQVW010000000
VERSION     DAOQVW010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197448_length_1096_cov_1.663785"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKK85_09900"
     CDS             complement(<1..540)
                     /locus_tag="PKK85_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNS26368.1"
                     /translation="MAEQEFAYFLGCIMNNRYPGIEKSTRILFDTLGVGLKDMEGASC
                     CPAPGVFGSFDRTTWASIAARNITIAEEQGNDIMTECNGCFGSLFESNHLLQENEEMK
                     EKINKVLKEAGSKEFKGEIKVRHFAEILYNDIGMDKLSEAITKPLNLNVAVHYGCHFL
                     KPSAEIGIDDPMKPTILDEL"
     gene            complement(550..>1096)
                     /gene="hdrC"
                     /locus_tag="PKK85_09905"
     CDS             complement(550..>1096)
                     /gene="hdrC"
                     /locus_tag="PKK85_09905"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HNS26369.1"
                     /translation="GREEDTLTRDSIDVNFKQEVMDAGADTLALCFQCGTCTGACPSG
                     RRTPYRIRQIVRKSLMGFKDDVISDDTLWMCTTCYECQERCPRGIKIVDIVKIVRNYA
                     AQAGYMGSAHKAVGSFVAKTGHGVPINDATRALRKSVGLDELPPTTHSFPGALEEVQN
                     IIKATGFDGLIGYNWEKGELE"
BASE COUNT          274 a          247 c          196 g          379 t
ORIGIN      
        1 caattcatca aggatggttg gtttcatagg atcgtcgatt ccgatttcag cactaggttt
       61 gaggaagtga catccatagt ggacagctac gttaagattt aatggtttag taatagcttc
      121 agacagttta tccattccaa tatcgttgta gaggatttct gcaaagtgtc ttactttaat
      181 ttcaccttta aactctttac taccggcttc tttaaggact ttgttaattt tttctttcat
      241 ttcctcgttt tcttgaagta ggtggttgct ttcgaaaagg gaaccgaaac atccattaca
      301 ttctgtcatg atgtcattac cctgttcttc agctatggta atgtttcgag cagcaatgga
      361 tgcccatgtg gttctatcaa atgatccaaa aacccctggt gcagggcagc atgaagctcc
      421 ttccatatct ttaagtccca cacctaatgt gtcaaagagt attctggtag atttttcgat
      481 accagggtat ctgttattca taatacatcc taaaaaatat gcaaattctt gttcggccat
      541 cttatcacct tattctaatt ctcctttctc ccagttgtaa ccaattagac catcgaaacc
      601 ggtggctttg attatattct gaacttcttc cagggctcca gggaagctgt gagtggttgg
      661 tggtaattca tccagtccca cacttttcct cagcgccctt gtagcatcgt tgataggcac
      721 tccgtgtcct gtctttgcaa caaatgatcc tactgcttta tgagcagatc ccatgtatcc
      781 tgcctgggca gcgtagtttc tgactatttt tacgatatcc acaatcttaa tgcccctagg
      841 acatctttcc tgacattcat aacaggttgt acacatccag agtgtgtcat ctgatattac
      901 atcatcttta aagcccatta atgattttct tactatctgt ctaattcggt atggggttct
      961 tcttccagaa ggacatgctc cggtacatgt accgcactgg aaacataatg ccaatgtgtc
     1021 agcaccagca tccattactt cttgtttaaa atttacatct atactatcac gagttagtgt
     1081 atcttcttct cttcct
//
LOCUS       DAOQVW010000602         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_197679_length_1095_cov_2.010577, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000602 DAOQVW010000000
VERSION     DAOQVW010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197679_length_1095_cov_2.010577"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKK85_09910"
     CDS             <1..235
                     /locus_tag="PKK85_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNS26370.1"
                     /translation="LWLLLGFKKSAEALGISQALVGQSTIITTAPWPTVDPLVIALPV
                     AVVVTIIVSYLTKPPEKEFLDRCFEGVDGGKGK"
     gene            247..1089
                     /locus_tag="PKK85_09915"
     CDS             247..1089
                     /locus_tag="PKK85_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS26371.1"
                     /translation="MKLKKYIYRKNKVENWTEVFQNPCQVTVRSFQTGTVKINRKGTL
                     NPDHPLAGDVQEEELEVPILVYWVRHKEKGDILLDAGLDASYITDPYGGLEGSSVDEF
                     KLGEHKNIAYHIQDHGIELKTVFLSHLHADHAAGVRELPRDIPYIISKGEYDEYQPEV
                     HGDFLEGLEELYEIDFSQATKIPPIGSSIDILGDGSLWTIWTPGHTPGHMSFLVNGRE
                     GPVLLTMDAAFIHENLETGVAPGDYTWNVEMAQETLEKIIEFLREYPQVRVGPGHGTL
                     KLSI"
BASE COUNT          332 a          221 c          265 g          277 t
ORIGIN      
        1 actatggctg cttttaggat ttaaaaaatc cgcggaagct ttgggaatct cccaagcatt
       61 agtgggacag tctaccatta taaccacggc accctggccc accgtggatc cattagtgat
      121 agcgcttcca gtggccgttg tggttaccat catcgtttct tacctcacca aacctccgga
      181 aaaggaattt ctggacaggt gcttcgaggg agttgatggg ggcaagggga aatagaaata
      241 gccgatatga aactcaaaaa atatatttac cggaagaata aggttgaaaa ctggactgaa
      301 gtattccaga atccctgcca ggtaacagtt cgaagtttcc agaccggaac agtgaagata
      361 aacagaaagg gaactttaaa tcctgaccat cccctggcag gagatgtcca ggaagaggaa
      421 ttagaggttc ctatactggt ctactgggtg cgccataagg aaaagggtga tattctttta
      481 gatgccgggc tcgatgcttc ttatattacg gatccctacg gtggattaga aggatcctca
      541 gttgacgagt ttaaactagg tgaacataaa aatatcgcat atcatatcca agatcatggt
      601 attgaactta aaacagtttt tttaagccac ctacatgcgg atcatgctgc aggtgtaaga
      661 gaactgccca gagacatccc ttatatcatc tctaagggag aatatgatga atatcaacct
      721 gaagtacatg gagatttcct ggaaggactg gaagaactat atgaaataga cttttcccag
      781 gccacaaaaa tacctcccat aggttccagt atagatattc taggcgacgg atctctttgg
      841 actatttgga caccgggaca caccccagga cacatgtcat tcctggttaa tggaagggaa
      901 ggtccagttt tactcaccat ggacgcggcc ttcatccatg agaacctaga aacaggagtg
      961 gcacctggtg attatacctg gaatgtggag atggcacagg aaaccctgga gaagataatt
     1021 gaatttttaa gggaatatcc gcaggttagg gtgggccctg gtcatgggac tttgaaatta
     1081 agtatttaag ctttt
//
LOCUS       DAOQVW010000603         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_197833_length_1094_cov_3.249278, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000603 DAOQVW010000000
VERSION     DAOQVW010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197833_length_1094_cov_3.249278"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKK85_09920"
     CDS             <1..517
                     /locus_tag="PKK85_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNS26372.1"
                     /translation="LIICGNGTDIKTLEESNIEDADVFVAATGNDEANLLSSILVKEY
                     EIPKLIARVSNPDHEDAFHKVGIDIVVSPELTAASYLEKLITRPKIADLIVVGRGDAE
                     LLDISIQNPRAIGKRVGDLSPTDDYIIAAIHHNGNITIPHDDTVLAKNEKISVLVKTG
                     AVKKVTSLFTK"
     gene            complement(614..>1094)
                     /locus_tag="PKK85_09925"
     CDS             complement(614..>1094)
                     /locus_tag="PKK85_09925"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HNS26373.1"
                     /translation="KESKHGTVDGLGILGVETSFGDVKKIISQSQGMIMGNGLFKGME
                     GKLVTGYELHEGITIIKDSKPLTRVIKGCGNHPQSQFDGAVQGMSTGTYFHGIFHNFN
                     FRRFFTDFLREKMGLEKLGFDMDHFQLNKGFSLERLAGIFEENVDMEKMEKILGIES"
BASE COUNT          310 a          248 c          208 g          328 t
ORIGIN      
        1 cctaataatc tgtggtaatg gaactgatat taaaaccctg gaagaatcca acatcgaaga
       61 tgctgatgtt tttgtggcgg ccactggtaa cgacgaggcc aaccttttat ccagtattct
      121 ggtcaaggaa tatgaaattc ccaaactcat tgccagggtg agtaacccag accatgagga
      181 cgcctttcat aaagtaggta ttgacatcgt ggttagcccg gagctaactg cggccagtta
      241 tctggaaaag ctcattaccc gccccaagat agctgatctt attgttgtgg gtcgaggaga
      301 tgctgaactt ctggacataa gtatccaaaa tcctagagca ataggaaaaa gggtgggtga
      361 tttgagcccc actgatgatt atatcatagc cgccatccac cataatggga atatcaccat
      421 accccacgat gacactgtgc tggccaaaaa cgagaagata tcagttttgg tgaaaaccgg
      481 ggcagtgaaa aaggttactt cgttattcac caaataatta tagaattttt attagaaata
      541 tggcttttga tgaacctttt taatttaaat cccatcccct catcctggta cgacacctaa
      601 ttcacttatc atctcaggac tcaatgccca atattttttc cattttctcc atgtccacgt
      661 tttcttcaaa tattccagct agacgttcca acgaaaagcc cttgttcaat tggaagtgat
      721 ccatatcaaa gcctaatttt tccaaaccca ttttttctct taaaaagtct gtgaaaaatc
      781 ttcggaagtt gaagttgtgg aatatgccgt ggaagtaggt tccagtggac attccctgca
      841 cagcaccatc gaattgggac tggggatgat ttccacaacc cttgataaca cgtgtaaggg
      901 gtttactatc tttgattatg gttattcctt cgtggagttc gtaacctgtg actaattttc
      961 cttccatgcc tttgaaaagt ccgttcccca ttatcattcc ctgactctgg cttatgatct
     1021 ttttcacatc accaaatgag gtttcaaccc ctaaaattcc taaaccatct acagtcccat
     1081 gtttcgattc tttc
//
LOCUS       DAOQVW010000604         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_198241_length_1093_cov_2.303468, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000604 DAOQVW010000000
VERSION     DAOQVW010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198241_length_1093_cov_2.303468"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKK85_09930"
     CDS             <1..401
                     /locus_tag="PKK85_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HNS26374.1"
                     /translation="HFIIEHFDSQPASLRLCYHRQRLFIMIVKDNLEKLGLKTIREGD
                     DLYHVIGSDKKAKLSVSIATSSHNSMKIHFAVNISSEGTPSDVEITSLNDCIEGMTME
                     DINQLADSIVDDYVSELSSIERDITKTKVF"
     gene            482..973
                     /locus_tag="PKK85_09935"
     CDS             482..973
                     /locus_tag="PKK85_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNS26375.1"
                     /translation="MKIVINGIHANLRFSAAHMIPCHEFCGGIHGHSYHVDVQVEGER
                     SGEFGFVVDFKKVKELTRDICKKLDHKLLIPLNNNEIKFKKLEESIEFSIREKEYKIP
                     AEDCTLLPLKSTSAEDMAEYFAEKLYSSLKADEAESNTKIKSVQVCVNEGIGQGAYFE
                     RLD"
     gene            982..>1093
                     /locus_tag="PKK85_09940"
     CDS             982..>1093
                     /locus_tag="PKK85_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019268974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNS26376.1"
                     /translation="MKTRINEVFSSIQGEGKLVGRRQVFIRFSGCNLDCNY"
BASE COUNT          387 a          187 c          234 g          285 t
ORIGIN      
        1 tacacttcat aatagaacat tttgactcac aacctgcaag tttaaggctc tgctaccaca
       61 ggcaacggtt atttatcatg atagtcaagg ataacctgga aaaactagga cttaaaacca
      121 taagggaagg tgatgatctt taccatgtga taggatctga caagaaagcc aagctcagtg
      181 tttccatcgc cacctcatcc cacaacagca tgaagataca cttcgcagtg aacatatcca
      241 gtgagggaac accctctgat gtggagataa ccagtcttaa tgattgtata gaaggtatga
      301 ccatggaaga tataaatcaa ctggcagata gtattgtgga tgattacgta tctgaattat
      361 cttccattga aagggacata accaagacga aggtatttta actgatatga ggatattcta
      421 aaccctatta aaaatgaaaa ttacatataa aaaaacgaaa ttaagtaaga aggttaattg
      481 aatgaaaata gttataaatg gtattcacgc taatttaagg ttctcagcag cccatatgat
      541 cccctgccac gagttctgcg gtgggatcca tggacattcc taccacgtgg atgtacaggt
      601 ggaaggggaa cggtccggtg aattcggatt cgtggtggac tttaagaaag ttaaggaatt
      661 aacgcgggat atatgcaaaa aactggatca taaactttta atcccactaa ataataatga
      721 aataaagttc aaaaagttgg aagaatcaat agaattctcc ataagagaaa aagagtataa
      781 gatccccgcc gaggactgca ctttactccc tttgaaatcc acttctgcag aggatatggc
      841 ggaatatttc gcggaaaaac tttacagttc acttaaagca gatgaagcgg aatcaaacac
      901 taagattaaa agtgtccagg tatgtgtaaa tgaaggaata ggacagggtg cctattttga
      961 aagactagat taggttaacc aatgaaaaca cgtattaatg aggttttctc cagtatacag
     1021 ggtgaaggta agctcgtggg caggagacag gtgtttataa gattttctgg ctgtaactta
     1081 gactgtaact act
//
LOCUS       DAOQVW010000605         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_199351_length_1089_cov_3.176983, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000605 DAOQVW010000000
VERSION     DAOQVW010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199351_length_1089_cov_3.176983"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKK85_09945"
     CDS             complement(<1..265)
                     /locus_tag="PKK85_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNS26377.1"
                     /translation="MINIEYVILLIAIGGIIYARRALDLAGSVFMVIMGIIIIFTAGF
                     NWLLLIFVFLILGLLLTRYQHNYKKDIGVYEGTRTIKNVVSNGI"
     gene            complement(281..865)
                     /locus_tag="PKK85_09950"
     CDS             complement(281..865)
                     /locus_tag="PKK85_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HNS26378.1"
                     /translation="MAKARRRRVRDTWKDKQWYKITTPKEFGESEIGTTPARDPNLLL
                     RRKVEATMRELSGDFSKQYVKLRFQIYDVAGDTAHTRYMGHQVTTDYVRSMIRRGTTR
                     IDAIIKLETKDGKNLMVHMLAITIKRAKSSQQRYIRETMEKLVREAAANKTLNELVED
                     IIKGKLASSVYHETKKIYPLKRVETIKSRVIEEK"
BASE COUNT          288 a          233 c          212 g          356 t
ORIGIN      
        1 cgatcccatt agaaaccacg ttcttaatag tccgggtccc ctcgtagact cctatatctt
       61 ttttataatt atgttggtac cgggttaaca acaatcccaa gattaagaag acgaagatta
      121 atagcagcca gttgaaacca gctgtgaaga tgatgattat ccccatgatg accatgaaga
      181 ccgagccagc cagatcaagg gcgcggcggg cataaatgat cccaccgatg gctatcagga
      241 ggattacata ttcgatgtta atcatgtctt ccttgtatta ttatttttct tcgataacac
      301 gggatttgat ggtttccacc cgtttcaggg ggtatatctt tttagtttca tgataaacac
      361 tggaagccag tttacccttg atgatatctt ccaccagttc attcagggtt ttattggccg
      421 cggcttctct gactaatttt tccatagtct cccgtatata acgttgctgg gatgatttag
      481 ctcttttgat ggttatggct aacatatgaa ccattaagtt cttaccgtct ttggtttcca
      541 gctttattat agcgtcaatt cgagtggttc cccttcgaat catactgcga acgtagtcag
      601 tagttacttg gtgtcccatg taccgggtat gagcagtgtc tccagccaca tcgtatatct
      661 gaaatcgtaa cttgacgtac tgcttactga agtccccact taattccctc atggtagctt
      721 ccactttcct tcgcagtagc agattagggt cacgggcagg agtggtgcct atctcagatt
      781 caccgaattc tttaggagtg gtaattttgt accattgttt gtctttccat gtgtcgcgta
      841 ctcttcttct tcttgcttta gccattctaa atcaccttga catggtattt aaataagtgg
      901 ataaaaagaa catttaggaa gtttccaatc attcctactt ctaaaaatcc tctaatcata
      961 cctctaattt taatctttta taaatatatc cgaatcccgg gactaggccc ggttaaagtt
     1021 aaggaggagg caaaaaaagg aaaacctcgt ccatgatttt actggtgtta aattaaaaat
     1081 ataaggtct
//
LOCUS       DAOQVW010000606         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_199969_length_1087_cov_3.467054, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000606 DAOQVW010000000
VERSION     DAOQVW010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199969_length_1087_cov_3.467054"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKK85_09955"
     CDS             <1..515
                     /locus_tag="PKK85_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNS26379.1"
                     /translation="GAGYNGVPSMAILDNLVPFIGGEEEKIESETLHLLGDFDGETVT
                     PAGFGLSASCHRVPVVDGHTEAVFMEMEQDFAVEDVKSAFRNFKGLPQKLGLHFAPPT
                     PIIVREEDNRPQPRMDRLTQKGMAVTVGRVRKDDVFPKSLKYVLLGHNTVRGAAGASI
                     LNAELINEIM"
     gene            567..>1087
                     /locus_tag="PKK85_09960"
     CDS             567..>1087
                     /locus_tag="PKK85_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNS26380.1"
                     /translation="MFESLTGIIYQWDVGFLLFINLGFKNPVLDYIVLVATNLGLPIF
                     WLIVCTMIFIFGGEKGRKVAVLCLIALSLGWLLTEFLKIIIDRPRPYSILDQVRAVAT
                     EDGHSFPSGHSVAVFIACTMFGLEYGYIPIFMGLAVLVAFSRIYLGVHYPLDVIFGAI
                     LGISCALMVRHWEG"
BASE COUNT          276 a          215 c          268 g          328 t
ORIGIN      
        1 ccggtgcagg atacaatgga gttccctcca tggccatact ggacaacttg gtgcccttca
       61 tagggggtga agaagagaag atagaaagtg agaccctgca cctcctggga gacttcgatg
      121 gagaaactgt taccccggct ggatttgggc tcagtgcctc ctgccacagg gtacctgtgg
      181 tggatggaca tactgaagct gtattcatgg agatggaaca agattttgcc gtggaggatg
      241 ttaaaagcgc ctttcgaaac ttcaaaggcc tacctcaaaa actggggctg cattttgctc
      301 cccctacacc aataatagtt cgtgaggagg ataaccgtcc tcagccacga atggatcgcc
      361 tcacccagaa aggaatggca gtcacagtgg gaagagttcg taaagatgat gttttcccaa
      421 aaagccttaa atatgttctt ttaggacata ataccgttcg aggtgcagct ggagcatcca
      481 ttttgaatgc tgaacttatc aacgagataa tgtaagtatc caaaactttt ttattttttt
      541 tgccaattaa aatagggaaa aatccaatgt ttgaatcatt aactgggatt atctatcagt
      601 gggatgttgg ttttttatta ttcatcaacc tgggattcaa aaacccggtt ttggattata
      661 tagtgctagt ggctaccaac ctgggccttc ccattttctg gttaattgtt tgcaccatga
      721 tcttcatttt tggaggagaa aagggacgta aagtggcggt tctctgttta atagccttga
      781 gcctagggtg gcttctgact gaatttctta aaattattat tgacaggccc cgaccctatt
      841 ctatcctgga ccaggtaagg gcagttgcta ctgaagatgg tcattctttt ccttcaggtc
      901 actcggtggc ggtgttcatt gcttgtacca tgtttggctt ggaatatgga tatatcccta
      961 tttttatggg actagcagta ttggtggctt tttcacgtat ctatctgggg gtgcattacc
     1021 ctctggatgt gatattcggg gcaatattag gcatttcatg tgccctgatg gttagacatt
     1081 gggaagg
//
LOCUS       DAOQVW010000607         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_200989_length_1084_cov_2.850340, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000607 DAOQVW010000000
VERSION     DAOQVW010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200989_length_1084_cov_2.850340"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..305)
                     /gene="gatC"
                     /locus_tag="PKK85_09965"
     CDS             complement(90..305)
                     /gene="gatC"
                     /locus_tag="PKK85_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn) amidotransferase subunit GatC"
                     /protein_id="HNS26381.1"
                     /translation="MKIEKEAEIILEKFSKVLENIKDLEETHYIVDNVNLSREDKAQE
                     KNPEKILRNADVDEEGNLIAEKGKWVR"
     gene            complement(342..>1084)
                     /locus_tag="PKK85_09970"
     CDS             complement(342..>1084)
                     /locus_tag="PKK85_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNS26382.1"
                     /translation="LSRVGVMFSGGIDSTILAHLSREMGVETILYTVGREDSADFKFA
                     HKSAEDMGLPIRLGKVTRDDVRDYLPRVLNAIEEFNLMKLGVAMPAYLAAEMAREDGI
                     KVMLSGQGADELFAGYHRYLKFYQEQGEEAQKNLNTDINNLYQVNLQRDDAVTMTSGV
                     ELRVPYLDLDVINMAMNIPMEYKINGTDDHLRKCILREVARDLGVPEENVKRPKKAAQ
                     YGSGIDKMLRRVLKDEDILKNEDIKFYI"
BASE COUNT          242 a          274 c          216 g          352 t
ORIGIN      
        1 tgttagctcc taatctccct atgggattta gtgcgtccag gagctggcct ggaacatcta
       61 aaagttctaa taccagtttt aacctcatct tatctgaccc attttccttt ttctgctatg
      121 agatttcctt cctcatcaac atcagcattt cgaaggattt tctcgggatt tttctcctgt
      181 gccttatctt cacgtgataa attaacatta tccacgatgt aatgggtttc ttcaagatct
      241 tttatgttct ccagtacctt ggagaatttc tcgaggatta tctctgcttc cttttcaatc
      301 ttcaattttc gaacactcct ctctgctttt agggattatc gttaaatgta aaacttgata
      361 tcttcgtttt taagtatatc ctcatcctta agtacccggc gaagcatctt atcaatgcca
      421 gaaccgtact gggccgcctt tttaggtctt tttacgtttt cttctggtac gcccagatcc
      481 cgggcaactt cccgtaggat gcacttccgg agatggtcat ctgtaccgtt gatcttatac
      541 tccataggta tattcatggc catatttata acatccagat cgaggtaggg aacccgcagt
      601 tccactccac tggtcatggt caccgcgtca tcccgctgga ggttaacctg gtacaggtta
      661 tttatatctg tgttaaggtt tttctgggct tcttctccct gttcctggta gaatttcagg
      721 tagcggtggt acccggcgaa gagttcatcg gcaccctggc cggatagcat gaccttgatc
      781 ccatcttcac gggccatctc cgcggctagg tatgctggca ttgccactcc taatttcatt
      841 aggttgaact cttctatagc atttaataca cgggggaggt aatctctgac atcgtccctg
      901 gtcaccttac ctaaacggat gggaagaccc atatcttccg cacttttatg tgcaaatttg
      961 aagtcagctg aatcttcacg tcccacagtg tacaagatgg tttctactcc catctcacgg
     1021 gagagatggg ccagtatggt gctgtctatc ccaccagaaa acataacacc tacccgggat
     1081 aggc
//
LOCUS       DAOQVW010000608         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_202128_length_1080_cov_4.907317, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000608 DAOQVW010000000
VERSION     DAOQVW010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202128_length_1080_cov_4.907317"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKK85_09975"
     CDS             <1..447
                     /locus_tag="PKK85_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS26383.1"
                     /translation="LVVSPTTSNTIAKVVSGIADNLITNAIAQAGKGKIPTLIVPVDL
                     EAGDVQTVLPSKLELELCQHCSTCHAAAACPQDAITPGVEINLLKCQGCGACQMACPF
                     GAISGGKKIFIRMREIDISNTKKLAGLEDVDVVETPEQLLKIISSF"
     gene            complement(607..888)
                     /locus_tag="PKK85_09980"
     CDS             complement(607..888)
                     /locus_tag="PKK85_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26384.1"
                     /translation="MEVKIVDYGVSKEFGKYYITYLVKGLNPHEISQVQIKLEDPLVV
                     KCDELYLTTFFDEEYYPFHSADAQARMEDYIAREEIEMTAYILDLLEDN"
BASE COUNT          298 a          240 c          231 g          311 t
ORIGIN      
        1 ctggtagtct ctcccaccac atccaacacc attgccaagg tagtgagtgg cattgcagat
       61 aaccttatta ccaacgccat tgcccaagca ggtaagggca agattccaac cttgatagtg
      121 ccggttgatc tggaagccgg cgatgtgcag accgtgctgc cctctaaact ggaacttgaa
      181 ttatgtcagc actgctcaac ttgtcatgcg gcggctgcct gtccacaaga tgccataact
      241 cctggagtgg aaattaacct gttaaaatgc cagggatgtg gtgcatgtca aatggcatgt
      301 cccttcggag caatttcagg tgggaagaag attttcatcc ggatgcggga gatcgatatt
      361 tcaaacacca aaaaattggc aggcctggag gatgtggatg tggttgaaac tccagaacaa
      421 cttttaaaaa tcatttcttc attttagatc tacatgctgg agaaatgcac taacccctcc
      481 ccctattatc taaataaatt ttgaaaagga atggattctt attagattag gaacctgaat
      541 tggatttatg ggtttaacaa cgtcactatg aagaattagg cacggaagga tattcatatg
      601 cataatctaa ttatcttcca gtagatccag tatataggcc gtcatttcga tttcttcacg
      661 tgcaatgtag tcttccatcc gggcctgtgc atcagcactg tggaagggat agtattcctc
      721 atcaaagaag gtggtgagat acaattcatc acacttcacc accaggggat cctccagctt
      781 gatctgtact tgggatattt catggggatt gagtccttta accaggtaag tgatataata
      841 cttcccaaat tccttcgaaa caccatagtc cacgattttt acttccatat tcaggcctcc
      901 caaataattg aacttcaaac catttcacag ttaaattcca gttcatcacc tatttccgtt
      961 gctgaattgg ttaaaacccc tgttttgaat tctataacga atctagcagg tgctcttggg
     1021 gtgtaagtct gccatggctt tagggtgacg gtgtccacta cggttttggc ttcatccagg
//
LOCUS       DAOQVW010000609         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_202282_length_1080_cov_2.145366, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000609 DAOQVW010000000
VERSION     DAOQVW010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202282_length_1080_cov_2.145366"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKK85_09985"
     CDS             complement(<1..196)
                     /locus_tag="PKK85_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4213 domain-containing proteins"
                     /protein_id="HNS26385.1"
                     /translation="MKLINELIQAASENNSPVKDVRVGVSWTGVHGKYGGVSKTYGIP
                     VVHGNYTRDMGELTSKTTLEL"
     gene            complement(206..778)
                     /locus_tag="PKK85_09990"
     CDS             complement(206..778)
                     /locus_tag="PKK85_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNS26386.1"
                     /translation="MSDFDILLEKGRAFHGEICPGIVIGTRIAMVGLRELGMDQFERN
                     RDLMVYVEIDRCMADAVQAVTGNTMGHRTLKYKDYGKFAATFLDLSSGKAVRVSAVEG
                     PRVSPKSDDAGKPDMKEMVEKLSKVPEKELLVIEEVKVDIDDHDLPGFPKFRAYCEEC
                     GDRVLDRREVIVDGRTLCKACAEGPYYQKL"
BASE COUNT          299 a          255 c          179 g          347 t
ORIGIN      
        1 ccagttccag agtggtttta ctcgttaact cccccatatc tcgagtgtag ttaccatgaa
       61 ctaccgggat cccgtaggtt ttggaaactc ctccatattt tccatgaacg cctgtccagg
      121 atactccaac ccgtacatcc tttacaggag aattattttc acttgcagct tggattagtt
      181 cgtttatcag cttcatctta tcatcttata atttttgata atatgggcct tcagcacagg
      241 ctttgcacaa cgttcgccca tcaactatga cttcgcgtct gtccaacact ctgtccccgc
      301 attcttcgca gtatgcccta aatttgggga agcctggaag gtcatggtcg tctatatcta
      361 ctttaacttc ctctattact agaagttctt tttcagggac cttggaaagt ttttccacca
      421 tttccttcat atctggtttt ccagcatcgt cagattttgg tgatacacga ggaccttcca
      481 cggcggaaac tctcaccgcc tttccagagg atagatccag aaaggtagct gcaaatttac
      541 cgtaatcctt gtatttaagg gtacggtggc ccatggtgtt cccggtcacg gcttgaactg
      601 cgtccgccat gcaacggtct atttccacgt agaccattaa gtcgcgattt ctctcaaatt
      661 gatccattcc cagttctctc agcccgacca tggctattct agttcctata actatacctg
      721 ggcaaatttc accatggaaa gccctgcctt tctccagtaa gatatcaaaa tcactcattt
      781 ttgcacttcc tttaattttt tctcttatgt tttaataaac atcttaattg aattttctga
      841 aaattataaa tttctaaata aagattttta tctaaattac atctacaata tgcttatata
      901 ttcacattgg aatataccaa acttggagct catttggagg tgagcctata aatcaatcta
      961 atgaaattca agataaatcc actaaaccta attggaacat tatatggaaa gaagcagttg
     1021 aaaaactccc taaaaaggac acttcaaaaa aatggaataa gattgcgccg aagttcaatc
//
LOCUS       DAOQVW010000610         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_203186_length_1077_cov_2.338552, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000610 DAOQVW010000000
VERSION     DAOQVW010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203186_length_1077_cov_2.338552"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKK85_09995"
     CDS             complement(<1..591)
                     /locus_tag="PKK85_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNS26387.1"
                     /translation="MKSIEIHPVTRIEGHAKITVQEDDAGNVTDAHFHVMEIRGFEKF
                     LEGAAVEEAPRITPRICGICQTAHHLSSAKATDQIFGLEPPQTAKNLRELMLLGQYIH
                     SHSLHFYFLGAPDLVLGPDSDPALRNVVGILKKDPELAKMAIATRKIGQEITGVVGGK
                     PISPVTAIPGGQSKGITSEEREKLSSRAKEAVGLIEQ"
     gene            complement(588..>1077)
                     /locus_tag="PKK85_10000"
     CDS             complement(588..>1077)
                     /locus_tag="PKK85_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HNS26388.1"
                     /translation="GCPPKERLVGDILIPLIHDEAPVVPKKSVCADCQRRMEHVEFDK
                     IHRRVEGDPDPEKCFLSQGYICLGSVTLGRCGALCTAAGVACHGCGGPSLDVLREPSH
                     DIYNGVIKRIAHLSKMPEKDVEKQMYDMGHLIYGFVIGSKIMEDKLVSKIPQLVKKEV
                     VR"
BASE COUNT          214 a          269 c          252 g          342 t
ORIGIN      
        1 ctgttctatt agtccaacgg cttctttagc tctgcttgaa agtttctctc tttcctcgga
       61 ggttatcccc ttggactggc ctcctggtat ggcggtaacc ggactgatgg gtttacctcc
      121 caccacacca gttatctctt ggcctatctt cctggtggcg atggccattt tagccagttc
      181 tggatccttt ttaagtattc ccaccacgtt tctcagggcg gggtctgaat ctgggcccag
      241 gaccaggtcg ggtgctccta ggaaatagaa gtgaagggag tgtgagtgga tgtactgtcc
      301 gagaagcatg agttccctta agtttttagc tgtctgggga ggttccaatc cgaatatctg
      361 atctgttgct ttggcagatg ataaatggtg tgctgtttgg cagataccgc agattctggg
      421 tgttatacgg ggtgcttctt ctactgccgc tccctcgagg aacttttcaa aaccgcggat
      481 ttccataacg tggaagtggg cgtcggttac attacctgca tcgtcttcct ggacggtgat
      541 cttggcatgt ccttcaatcc tggttactgg atgtatctct atggatttca tcggaccacc
      601 tccttcttaa ccagctgtgg tatcttgctt actagtttat cttccattat cttacttcct
      661 ataacgaaac cgtaaattaa gtgtcccatg tcgtacatct gtttttccac atccttttct
      721 ggcatcttgg ataagtgggc aattctcttt atcactccat tgtagatgtc gtggcttggc
      781 tcacgtagga cgtccaggga tggtcctcca catccgtggc aggccactcc tgctgctgta
      841 cataatgcac cgcatcttcc cagggttact gagcctaaac agatgtatcc ctggcttaag
      901 aagcattttt ctgggtcggg atctccttcc accctgcggt gtatcttatc gaattccacg
      961 tgttccatgc ggcgctggca atcggcacag acactctttt taggaactac aggtgcttca
     1021 tcatgaatta agggtattaa aatatctcct accaatctct ctttaggagg gcatcct
//
LOCUS       DAOQVW010000611         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_203308_length_1077_cov_1.658513, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000611 DAOQVW010000000
VERSION     DAOQVW010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203308_length_1077_cov_1.658513"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PKK85_10005"
     CDS             complement(<1..1056)
                     /locus_tag="PKK85_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNS26389.1"
                     /translation="MVSATVGLIFIAYGVWRYRLPILTPAVAADKIISTMSNFLIILN
                     TQKKIVLVNQSALNLLNYKREDLIGKSSSLIFRLKEGVWDIFQNINLDEIGQVKTFET
                     EIKSSENKFIPVLMSVSLIKINENEPLGLVCIGSDLTKEKEIKKALKESEILYSTLIK
                     TDPDAIITTDLNGKISYTSKQTLEMYGYENKDELIGISIFDLVADAYKEKAKEDMEKT
                     KNENVLRNLEYNFVKKNGDQFIGEWNAAVIKNPENKPTAFMITARDITHHKNIEVQMQ
                     ASIQEKNVLLKEIHHRVKNNLQIISSLLNLQSRYIEDEKAYEVFKESQNRVKSMAMVH
                     EKLYQTGNFAELSVAEYL"
BASE COUNT          284 a          218 c          175 g          400 t
ORIGIN      
        1 caggtattca gccacactta attcagcaaa atttccagtt tggtacagtt tctcatggac
       61 catggccatg gatttaaccc ggttttggct ttccttgaaa acttcatagg ccttctcatc
      121 ctctatatat ctggactgca agttcaacag gctggatatg atttgtaagt tgtttttcac
      181 cctgtggtga atctccttta aaagcacgtt tttctcctgg atggaagcct gcatctggac
      241 ttctatgttt ttatgatggg ttatatctct ggcagtgatc atgaatgctg ttggtttgtt
      301 ttcagggttc ttgatcaccg cagcattcca ttcgcctata aattggtccc cattcttttt
      361 aacaaagtta tattccaggt tccttaagac attctcgttt tttgtttttt ccatgtcttc
      421 cttagccttc tccttgtatg catcagctac caaatcaaaa attgaaattc caatcaactc
      481 atctttgttt tcatatccat acatttcaag tgtctgttta gatgtgtaac ttatcttacc
      541 attcaggtcg gtagtgatga ttgcgtctgg atccgtttta atcagggtgg agtatagaat
      601 ctcagattcc tttaatgcct tctttatttc cttttcttta gttagatcac taccaatgca
      661 tactagacct aaaggttcat tttcattaat cttaattaaa gacacagaca ttaaaaccgg
      721 tataaatttg ttttcactgg attttatttc ggtttcaaag gtttttacct gccctatttc
      781 atccagattt atattttgaa atatatccca tacaccttct tttaatctga agattaaact
      841 agaagattta ccaattaaat cctccctttt ataatttaac aggttaagcg cactttgatt
      901 taccagcaca atcttttttt gggtgtttaa aataattaaa aagttggaca tggttgatat
      961 tatcttatcg gcagccacag caggggttaa gatcggtaaa cggtatctcc ataccccgta
     1021 agctataaag atcaaaccaa ctgtagcact gaccatcgtt atctcaggga atcgtat
//
LOCUS       DAOQVW010000612         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_203407_length_1076_cov_3.473066, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000612 DAOQVW010000000
VERSION     DAOQVW010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203407_length_1076_cov_3.473066"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..388)
                     /locus_tag="PKK85_10010"
     CDS             complement(179..388)
                     /locus_tag="PKK85_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26390.1"
                     /translation="MNKVLILVLVVLVMLAAVLFMARNGSDETENNNNSAEAQNTQNM
                     PYIQDSNHYSTPPQSSGGSNSEKVA"
     gene            481..873
                     /locus_tag="PKK85_10015"
     CDS             481..873
                     /locus_tag="PKK85_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26391.1"
                     /translation="MKMTEHLDPYQERIKMLESELSKRDKELESFKEILSTSQEILRD
                     AMDDKKIIQKRLDELEHMKVEFNVRRYHKLQNDHDKLQHRYQVTKKLLEEARLVIKDL
                     ENRGLMDYILKRYPDSFLEFNKDDPKIK"
     gene            complement(897..>1076)
                     /locus_tag="PKK85_10020"
     CDS             complement(897..>1076)
                     /locus_tag="PKK85_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26392.1"
                     /translation="LNTNTQFLTVNAQAAVEPETVNAQTETKTVRMQKTGIPVNYLLL
                     ALLLVIGGYIVPKRK"
BASE COUNT          335 a          189 c          243 g          309 t
ORIGIN      
        1 aaacagatta gcacccggat accctcgcca tttgccttca acctggtggc ccagggctac
       61 ctggatgttc tgaagtatga agagaggatg gagttcataa ggaggatgca ccaagccata
      121 ttagaggaga tagggagttc taagtagaaa aaatttattt tgtattaata ctaggagttc
      181 atgccacttt ttctgaattg ctccctcctg aactctgtgg tggagtgcta taatggttag
      241 agtcctggat atagggcatg ttctgggtgt tctgtgcctc tgcagaattg ttattatttt
      301 cagtttcatc agaaccgttt ctggccatga ataggacagc agccaacatt accagaacca
      361 ccaggaccaa gatcaacact ttgttcattc taaagcacct actaccatta ttagagtgaa
      421 gggtaaaata gtttacctaa gaatcagggc catgatcctc tcaatttcat aggaagtgga
      481 atgaagatga ctgaacactt ggatccgtac caggaaagaa taaagatgct ggaatctgaa
      541 ctctcaaaaa gggataaaga gctggagtcc tttaaagaga tactatccac ttctcaggag
      601 atcctccgtg acgccatgga tgataagaag ataatacaaa agcgtctgga tgagttggaa
      661 catatgaagg tggagttcaa cgtgcgaaga tatcataaac tccaaaatga ccatgataaa
      721 ctgcaacacc gctatcaggt aactaaaaaa ttgctggaag aggctcgact ggtaattaaa
      781 gacttggaga ataggggttt gatggattat attcttaaaa ggtatcctga tagtttttta
      841 gagtttaata aggatgatcc taaaataaaa taaaaataaa aaaaaatgga aatggtttat
      901 ttcctttttg gcactatgta accccctatt actagaagta aagctaacaa taggtagttt
      961 acgggtatac ctgttttttg catccttacc gtcttagttt cagtctgggc gttcactgtt
     1021 tctggttcga ctgctgcttg agcgttgact gttaagaact gggtgttggt gtttag
//
LOCUS       DAOQVW010000613         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_203691_length_1075_cov_5.504902, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000613 DAOQVW010000000
VERSION     DAOQVW010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203691_length_1075_cov_5.504902"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..876
                     /locus_tag="PKK85_10025"
     CDS             220..876
                     /locus_tag="PKK85_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26393.1"
                     /translation="MHSKTGNQTPKGTKQINKSHELNITYSKGTDKQKVLITDSTGPL
                     PGRGRYYQKPPSPYPCTNPTTKPETPLSKHYTGWQRCSYQGHYFKKSKRKRALKYCND
                     ICQEIERWRQRHTTTTGAPDPDPLTPEQLRRAKINYNMKMAFGPQVKIPGQKPKHRME
                     YIDPQEVEDFKLFLEKELHREHLEALELKEWIKPITKVFQEETPPEGPPPASPEPVPA
                     "
BASE COUNT          359 a          234 c          224 g          258 t
ORIGIN      
        1 ataattagga gataatattt attacattaa gttcacttga cgggttctaa aaagctcctt
       61 agaaaccgtt aaagataaat ataatgtaat aaatacttag acatgggaaa ggaaactttc
      121 cttaaccggg ggattaaata tggttttgaa ttgccaagca cgaaaaggaa aagagaacgc
      181 attatttgat ctgatattca gatcaaaggg aaagagacca tgcactcaaa aacaggcaac
      241 caaaccccga aaggcaccaa acagattaat aaaagccatg aattgaatat tacttattct
      301 aaaggaacgg ataagcaaaa ggttttaata accgactcca ccggaccact accaggccgg
      361 gggcggtact accagaaacc accctcacct tatccatgca ccaatcccac caccaaacca
      421 gaaactccat taagtaagca ctacacggga tggcagcggt gcagctacca ggggcattat
      481 tttaagaaaa gcaaaaggaa acgagccctt aagtattgta atgacatctg ccaggagatc
      541 gagcggtgga gacaacgaca caccaccacc acgggggcac cggatccgga tccattgaca
      601 cctgagcagc ttagaagggc caaaattaat tataatatga agatggcttt tggaccccag
      661 gtcaagatcc caggtcaaaa accaaaacat cgaatggaat atattgaccc ccaggaggtg
      721 gaggatttta aactatttct tgaaaaagaa ctccaccggg aacacttgga agccctggaa
      781 ctaaaggaat ggattaaacc aataactaaa gtttttcagg aggaaacacc accagaaggg
      841 ccaccaccag cgagccccga gcctgtgccg gcctgagggg gtgttttggt tttgtctgaa
      901 atgtgccctt actagtgagt gcccccttac ttcatataat tgagactaag attcccggcc
      961 gttttcttca aaagtctctc tttttgtggt gggggtgttc ctttccctat cacatcacct
     1021 ttttttttat actcaaatcc tttttgtttg aattaatttt ttaataggag gtgaa
//
LOCUS       DAOQVW010000614         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_204359_length_1073_cov_3.354617, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000614 DAOQVW010000000
VERSION     DAOQVW010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204359_length_1073_cov_3.354617"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKK85_10030"
     CDS             <1..524
                     /locus_tag="PKK85_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNS26394.1"
                     /translation="LVEVMSLMLTNEEHHYHKMGLPEVDRVEVAQPISQDRTMIRQSL
                     LNGLLEFLDHNRHEDLPQGLFEVGEVVYLDGEAETRSRGVRKLAACLTHSHANFTEIK
                     SLTAAVLSNLGLKMEVEITEHPSFLRGRCSRVTGRRNGKPILDGIFGEMDPAVVTSFG
                     LEYPVVALEVKFQ"
     gene            complement(623..958)
                     /locus_tag="PKK85_10035"
                     /pseudo
     CDS             complement(623..958)
                     /locus_tag="PKK85_10035"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
BASE COUNT          261 a          287 c          282 g          243 t
ORIGIN      
        1 gcctggtgga ggttatgagc ctcatgctaa ccaacgagga acaccactac cataagatgg
       61 gactacctga ggtggatagg gtggaggtgg cccagcccat cagccaggac cggaccatga
      121 tccggcagag cctccttaat ggtttactgg aatttctgga ccataaccgt cacgaggacc
      181 ttccccaggg tctatttgag gtgggcgagg tggtctacct ggatggtgaa gcagagaccc
      241 gtagcagggg ggtgcggaag ctggctgcct gcctcaccca cagccacgcc aacttcaccg
      301 agatcaaatc actcaccgcc gcggttttaa gtaacctggg actgaagatg gaggtagaga
      361 taaccgagca cccctccttt ttaaggggaa gatgctccag ggtaacggga cgaagaaatg
      421 gcaaaccaat cctggatggc atcttcgggg agatggaccc ggctgtggtt accagcttcg
      481 gattggagta cccagttgtg gccctggaag tgaagttcca gtaagaacca ttaaaaaaaa
      541 atggggaccc attttaggtg cctattggca acctgtttcg aatatttttt ttataaaaaa
      601 aaattattta atgggggggg tgttagaagg ctccaccccc acctcctcca aagccgccgc
      661 ctacgtcccc gacacctccg aagtcaccgc catcggatcc gtcagctgca ctggcagtgg
      721 tcattccagt gtccagggag tcagccagga gcacgtagcc accgtagtgg tggaacatgt
      781 aaatctcgct atcctccaac tcctcgtggg gtagtgatag ttccatggcc ttgcgcactg
      841 catcggcaac gcccagtgcc gtggcataaa caaggtactt attccatatg gccacagatt
      901 cgggtgggta ctcctttatc aggctgaaat ccttgatgta cttcttgaag ttttgccact
      961 tagcatcgta ttcctctcaa taagtggtcc actggcctgc tatcttctgg ggcattatta
     1021 gggacactat acccaccact cccagtacca cggatgatat ccacgccacc cat
//
LOCUS       DAOQVW010000615         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_204650_length_1072_cov_3.442478, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000615 DAOQVW010000000
VERSION     DAOQVW010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204650_length_1072_cov_3.442478"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /gene="cofG"
                     /locus_tag="PKK85_10040"
     CDS             <1..994
                     /gene="cofG"
                     /locus_tag="PKK85_10040"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HNS26395.1"
                     /translation="EVNSSNDSRVTYSKNVFLPISNVCRNDCGYCTFRQNPQDPKTNL
                     LMYPEEVMKIIGHGHDRGCREALFTFGEHADENPLVLEALKKVGHETILDYLYFLCEE
                     TLNKTNLLPHSNLGILEKSELKMLKEVNASMGLMLETTSERIMKTVAHQHSPGKDPKK
                     RIKTIKNAGKLKIPFTTGLLIGIGETVEERAEALLEIRKIQDKQGHIQEIIIQNFCSK
                     PGIAMEDHPEPSLADMIRTVAAARILFPDCGVQVPPNLNLEVAQIFLLTGTDDWGGIS
                     PLTPDHVNPEAPWPELKELKSLTEDLGFKLRERLPVYDKFINSQYLSDVVLENI"
BASE COUNT          357 a          222 c          227 g          266 t
ORIGIN      
        1 ggaagttaat tcctcaaatg attccagagt aacttattct aagaatgttt tcctacccat
       61 tagcaacgtg tgccgcaatg attgtggata ctgcactttt cgtcagaacc ctcaggatcc
      121 caaaaccaac ctgctaatgt atccagaaga agtaatgaaa ataatcggcc acggtcatga
      181 tcgtggctgc cgggaggcac tttttacttt tggagaacat gctgatgaaa acccattggt
      241 acttgaagcc ctgaagaagg tgggacatga gactatttta gactatttat atttcttgtg
      301 tgaagaaact cttaacaaaa ccaacctgct tccccacagt aaccttggaa ttcttgaaaa
      361 gagtgaatta aaaatgctca aagaggtcaa cgcctcgatg ggattgatgc tggagaccac
      421 cagtgaaaga atcatgaaaa ccgtggccca ccagcacagt ccagggaaag atcctaaaaa
      481 aaggattaaa accattaaaa atgcggggaa gcttaaaata ccctttacga ctgggcttct
      541 aattggtatt ggagaaacag ttgaggaaag agcagaggcc ctcctggaga taagaaagat
      601 ccaggataaa cagggtcata tacaggagat tatcatccag aacttctgca gcaaaccagg
      661 aatcgccatg gaagatcatc ccgaaccatc cctggcagat atgatcagaa ctgtggctgc
      721 tgctagaatc ctctttcctg attgcggagt tcaagtaccg ccgaatctca acttagaagt
      781 ggctcaaata tttttactga ctggaacaga tgattgggga ggaatatcac cccttacccc
      841 tgaccatgtc aatccagaag cgccctggcc agaactaaaa gaattaaaat ctttaactga
      901 agatttaggc ttcaaattaa gagaaaggct gccagtgtac gataaattca taaattccca
      961 gtatctaagt gacgttgtcc tggaaaacat ctgataaaaa gaaaacgttt agctgcacat
     1021 gtattctaaa tcagtgccac ttgatagtta tggttaaagg tgtaaactaa tc
//
LOCUS       DAOQVW010000616         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_204698_length_1072_cov_2.729597, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000616 DAOQVW010000000
VERSION     DAOQVW010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204698_length_1072_cov_2.729597"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PKK85_10045"
     CDS             <1..749
                     /locus_tag="PKK85_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HNS26396.1"
                     /translation="FRAIEKTPKSSRGEYEITDSIKIQIQNGEEVLGLTFQDRWIDIG
                     RPWELLEINEHFLENMESEIQGIIEEGVTIHGPVILGKGSILRSGSYIMGPVFIGENT
                     DIGPNSFIRKYSSIGNGVSVGNGVEIKNSIVMDNTNINHLSYVGDSVIGADCNLAAGT
                     NIANLRFDDCEVKLTVKKDRINSGRRKLGVIFADDVKTGINSSFNPGVMVGQGARVGS
                     GAIIYRDVPSHMMVIPLQEHDIIDRRTMDK"
     gene            761..>1072
                     /locus_tag="PKK85_10050"
     CDS             761..>1072
                     /locus_tag="PKK85_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNS26397.1"
                     /translation="MKIIHPSVKIMSGAHLVGCVKMDEHSSVWYNAVLRGDLEPIIIG
                     KYSNIQDNCVLHSSQSYPLELGDYVSVGHAAVLHGCRVKDNCLIGMNATLLNGSRIGK
                     NS"
BASE COUNT          319 a          189 c          264 g          300 t
ORIGIN      
        1 tatttcgagc cattgaaaag actcccaaat cttctcgagg agaatatgag attactgatt
       61 ccattaaaat tcagatccag aatggtgagg aagtactggg actaaccttc caggatcggt
      121 ggatagatat tggtcgtccc tgggaacttt tagagattaa cgaacacttc ctggagaata
      181 tggaatctga aattcaggga ataatagagg aaggagttac cattcatggc cctgttatcc
      241 tgggtaaggg tagcatattg cgttcaggtt catacatcat gggaccagtt tttattggtg
      301 aaaacacaga tattggacct aactctttca tcaggaaata ttcctccatt ggaaatggag
      361 tcagtgtggg aaatggcgtg gagatcaaaa attccattgt gatggataac acgaatatta
      421 atcatctcag ttacgtgggg gattcggtta ttggggctga ctgtaaccta gcagcaggta
      481 ccaacattgc taatctgcgt tttgacgatt gcgaagtaaa gttaactgtg aaaaaggatc
      541 ggattaactc tggacgccga aaactgggag ttatatttgc ggatgatgtt aaaacaggta
      601 ttaattccag ttttaatcct ggagttatgg taggtcaggg tgcccgggtg ggatcaggag
      661 ccattatcta ccgggatgta ccctcacaca tgatggtcat tcccctgcag gaacatgata
      721 tcatagaccg gagaaccatg gataagtagt tactcttatt atgaaaataa ttcacccctc
      781 agtaaagata atgtccggcg ctcatctggt gggctgtgtg aaaatggacg aacactcatc
      841 agtttggtat aacgcagtat taaggggaga cttggaaccc attatcattg gaaagtattc
      901 aaatatccag gataactgtg ttttacactc ttcccagagt tatccgctgg aattggggga
      961 ttatgtatca gtaggtcatg ctgcagtgtt acatggctgt agggtaaaag ataactgtct
     1021 tattggcatg aacgccaccc ttttaaatgg tagccggata ggaaaaaaca gt
//
LOCUS       DAOQVW010000617         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_204728_length_1072_cov_2.471976, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000617 DAOQVW010000000
VERSION     DAOQVW010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204728_length_1072_cov_2.471976"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PKK85_10055"
     CDS             complement(<1..1071)
                     /locus_tag="PKK85_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNS26398.1"
                     /translation="MIGLGNCASSLIQGIYYYQDKKAEDAIGLMHWDIGGYLPSDLEV
                     VAAFDIDQRKVGKDVNEAIFAPPNCTHVFCPDLAPSGVKVEMGQVLDGVATHMAEFDD
                     NHTFIVSKEAEKSKKEIVEILKSRNVEILVNYLPVGSEEAVRFYAECALEAGVAFINS
                     MPVFIVSDPVWAARFEEKGIPIVGDDIKAQIGATITHRILANLFRERGVKLERTYQLN
                     TGGNTDFLNMLNRNRLDSKKESKTEAVQSVLGKRLDSENIHIGPSDYVPWQKDNKLCF
                     LRMEGKTFGDVPMNIELRLSVEDSPNSAGCVIDAIRCCKLALERGIGGQLTSISAFTM
                     KHPPEQYTDDLAREMVDEFIAGK"
BASE COUNT          270 a          265 c          220 g          317 t
ORIGIN      
        1 tttcccggca ataaactcat ccaccatctc cctggccaga tcatcggtgt actgctcagg
       61 ggggtgtttc atggtaaatg cagatatgga agttaattgt ccaccaattc cacgttccag
      121 ggcaagtttg cagcagcgaa ttgcgtcaat gacacaacca gcagagttgg gtgaatcctc
      181 cactgaaagt cgtaattcaa tgttcatggg gacgtctccg aaggttttac cctccatacg
      241 aaggaaacag agcttgttat ccttctgcca aggcacgtag tcactgggac cgatgtgtat
      301 attttcagaa tccagtctct ttcccagcac tgattgaact gcttcggttt tagactcctt
      361 ttttgagtcc agacggttgc ggttaagcat gtttaaaaaa tccgtgttcc caccggtgtt
      421 aagttgatag gtacgttcca acttgacgcc cctctcccgg aaaaggttgg ccaagattcg
      481 atgagtaatg gtagcaccta tctgtgcctt tatatcatcc cctacaatag gtatcccctt
      541 ttcttcaaat cgggcagccc atactggatc acttacaata aataccggca tggagttgat
      601 gaaagctact ccagcctcca gggcacattc tgcatagaaa cggacagctt cttcggatcc
      661 caccggcagg tagttcacca gtatttccac atttctgctc tttaaaatct caacgatctc
      721 ttttttactt ttttctgcct cttttgaaac tataaaggtg tgattatcat caaattcagc
      781 catgtgggta gctactccat ctaatacttg tcccatttca actttaaccc cggatggtgc
      841 taggtcaggg caaaaaacat gggtacaatt tggtggagcg aatatggctt cgttcacatc
      901 tttaccaacc ttgcgctggt ctatatcgaa ggctgccacc acttccaagt cagaaggcag
      961 atatcctcca atatcccagt gcatgagtcc gatagcatct tctgcttttt tatcttggta
     1021 gtagtatatt ccttgtatga gtgagcttgc acaattgcct aagccaataa tc
//
LOCUS       DAOQVW010000618         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_204866_length_1072_cov_1.724680, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000618 DAOQVW010000000
VERSION     DAOQVW010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204866_length_1072_cov_1.724680"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="PKK85_10060"
     CDS             <1..147
                     /locus_tag="PKK85_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26399.1"
                     /translation="NHKTLIIPGLGAPVSGEIEDESGWEVLVGPRDSSAAPDFLVKLK
                     QEAK"
     gene            181..654
                     /locus_tag="PKK85_10065"
     CDS             181..654
                     /locus_tag="PKK85_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNS26400.1"
                     /translation="MKTLMVVDPQMCTECQDCINACKKEHGISRAKKTSTVPIFCLQC
                     HPEKAFCARICPTGAIREKNGTLMVDEDTCILCRLCLIACPVGMMVIDSENKTMQKCT
                     LCMDSDCIIPACVEACKDNVLKVFSIEELEELKGKVEYTDFLNEVMKDFQGKNNH"
     gene            complement(674..>1072)
                     /locus_tag="PKK85_10070"
     CDS             complement(674..>1072)
                     /locus_tag="PKK85_10070"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNS26401.1"
                     /translation="YKGKKVDFDLDMLELDVDKSNTNLPVKSTFQRDVLLETYNIPYG
                     EVVTYKSLAEKLRSRAYRAVGTVMAKNPYPLVVPCHRVVKSDLTIGQYGGGRKMKREL
                     LKKEGVHLKGDKIIKSKKKGLFNKKIIKRG"
BASE COUNT          308 a          229 c          229 g          306 t
ORIGIN      
        1 aaccataaaa cactcatcat acctggtctg ggagccccgg taagtggtga aatcgaagac
       61 gaaagtggtt gggaagttct ggtaggaccc agagactcca gtgcagcacc tgatttcctg
      121 gttaaactca agcaagaagc caagtagatt ccctaaaaaa tattcaaaag cagtgattat
      181 atgaaaacgt tgatggtagt agaccctcag atgtgcaccg aatgccagga ctgtataaac
      241 gcctgtaaga aggagcacgg aattagcagg gcaaaaaaga ccagcacagt acccatcttc
      301 tgcctgcagt gccacccgga gaaggcgttc tgcgcccgca tatgccccac cggggctata
      361 agggagaaaa atggcacttt gatggtggat gaagatacct gcatactctg cagactgtgc
      421 ctgatagcct gtcccgtggg tatgatggta atagacagtg aaaacaagac catgcaaaaa
      481 tgtaccctct gcatggactc cgactgcatc atacctgctt gtgtggaggc ctgtaaggac
      541 aacgtcctta aagtcttttc catagaggag ctcgaggaac ttaaaggaaa agtggaatac
      601 actgatttct taaatgaggt tatgaaggat ttccagggta aaaataatca ttaattcttt
      661 tttggttata aaattaacct ctttttatta tttttttatt aaataaccct ttttttttac
      721 ttttaattat tttatcacct ttaagatgga ctccctcttt ttttaacagt tccctcttca
      781 tcttccgacc cccaccatac tgtccaatgg ttaaatccga tttaaccacc cggtggcaag
      841 gcacaactaa gggatatgga tttttagcca ttacggtacc aacagcccgg taggcccggc
      901 ttctgagttt ttctgccagg gatttgtagg ttactacctc tccgtagggg atattataag
      961 tttcaaggag tacatccctc tggaaggttg atttaaccgg taaatttgtg ttggatttat
     1021 ctacgtctaa ttctagcatg tccaggtcaa aatcaacttt ttttccttta ta
//
LOCUS       DAOQVW010000619         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_205009_length_1071_cov_2.834646, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000619 DAOQVW010000000
VERSION     DAOQVW010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205009_length_1071_cov_2.834646"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..332)
                     /locus_tag="PKK85_10075"
     CDS             complement(153..332)
                     /locus_tag="PKK85_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HNS26402.1"
                     /translation="MEFLKDEGGQGAAEYILLFGGVIVIAIAALYIYYTYMQGSTQLK
                     ATQDISTVRTSAKIG"
     gene            620..>1071
                     /locus_tag="PKK85_10080"
     CDS             620..>1071
                     /locus_tag="PKK85_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNS26403.1"
                     /translation="MKALFIPCGIGMGHASRSLAVAQKLEHDGFEVLFASYGEGYHFL
                     GEFTDYQPVELPEIRFYGGAGELDLKYTARKSMDVPFTFLRSIYHESKIIKKFQPDVV
                     VADSHYSAPLTCKVLGVPCVLISNELQFNFKELYPHDRKMEYLETGLER"
BASE COUNT          287 a          234 c          220 g          330 t
ORIGIN      
        1 actccaccga atagtaggat gtattcagct gctccttgtc caccttcatc tttaaaaaaa
       61 tccattccaa tcaccaccct ttgataaaag gttagaaaaa ataaataaaa cttgcgtcag
      121 ttagacgcag gttctattcg ataggattta atttatccta ttttggcgct ggttcttaca
      181 gtactaatgt cctgtgtggc ttttaattgg gtagatcctt gcatgtaggt gtagtatatg
      241 tagagagctg ctatggctat gacgattact ccaccgaata gtaggatgta ttcagctgct
      301 ccttgtccac cttcatcttt taagaactcc atattttttt cctccatgtg tagtttattc
      361 tatgaatgtc aatattgtta tgtatgacta ctcattaata aatgtttggt tttttttata
      421 ttttttgatg aatgttgttc tcaaatatca acccccacct tgccagtgtt gagtactatt
      481 attcacgaac tattattttt acttttggga tgtattttgg tacataccgg gctgggaatg
      541 attcatacta gatacagatc ctaggaataa ttcacaattt taaagcatta ttaatcacaa
      601 gcactgatag gttaacaaca tgaaggcact cttcataccc tgcggtattg gaatgggaca
      661 tgcatcccgg tccctggcag tggctcagaa actagaacac gacggcttcg aagttttatt
      721 cgccagctac ggcgaggggt accactttct aggggaattc acagattacc agccggtgga
      781 gcttccggag atcaggttct acgggggtgc cggggaattg gacctcaagt acaccgcccg
      841 taaatccatg gatgtcccct tcacctttct caggagcatc taccacgagt ccaaaataat
      901 aaaaaaattc cagcccgatg tggtggttgc cgactcccac tactcagcac ccctcacctg
      961 taaggtgttg ggagtgccct gtgtacttat cagtaacgag ttgcagttca acttcaaaga
     1021 actctacccc catgatcgga agatggagta cctggagaca ggtctggaac g
//
LOCUS       DAOQVW010000620         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_205520_length_1070_cov_1.937931, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000620 DAOQVW010000000
VERSION     DAOQVW010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205520_length_1070_cov_1.937931"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKK85_10085"
     CDS             complement(<1..271)
                     /locus_tag="PKK85_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26404.1"
                     /translation="MFEGHLEISGRSLPQTILGTSPFTAAPHFGHRTWLYQLDLYNQP
                     ENILKVMRKCYDLGVWGIQLIPDPPVVDALSWALEEGCVFSIVGTV"
     gene            complement(335..676)
                     /locus_tag="PKK85_10090"
     CDS             complement(335..676)
                     /locus_tag="PKK85_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNS26405.1"
                     /translation="MVRVEEFILASSNYVPGYEIVDTKGFVYGLTVRSRGVGGQIGAG
                     IRSMFGGEIKEYVSMMEDTRDEALQRAIDHAQEYGANAIISIRYDSNDISDVMQEILA
                     YGTAVVARKIE"
     gene            854..>1070
                     /locus_tag="PKK85_10095"
     CDS             854..>1070
                     /locus_tag="PKK85_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953898.1"
                     /note="catalyzes the formation of 3-phosphonooxypyruvate
                     from 3-phospho-D-glycerate in serine biosynthesis; can
                     also reduce alpha ketoglutarate to form
                     2-hydroxyglutarate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNS26406.1"
                     /translation="MKVLIADQINEKGIDELKDIADVVVQTDITPEELVSSIPDYDAI
                     VVRSRTKVTQEVIEASQKLKIIARAGVG"
BASE COUNT          331 a          232 c          210 g          297 t
ORIGIN      
        1 gtaccgtacc cacaatacta aacacacatc cttcttccag tgcccagctt aaagcatcca
       61 ccactggtgg atcggggatc agctgaatcc cccacacacc cagatcatag catttcctca
      121 ttaccttaag aatgttttct ggttgattgt aaaggtccag ctggtacagc caggttcggt
      181 gtccgaagtg tggtgcggca gtaaagggtg aagttcccag aatagtctgg ggtaagcttc
      241 taccactgat ttcaaggtgt ccttcaaaca taggggtaac ctctaatatg ggaaaatttc
      301 aagcaataaa aaaaaattat tagttaaata taatttattc aatcttccga gctacaacag
      361 ccgtgccgta ggccaatatt tcctgcataa catctgaaat atcatttgag tcgtacctga
      421 tactgattat ggcgttggca ccgtattcct gggcgtgatc tatagctctc tgcagtgctt
      481 cgtccctggt gtcctccatc atactcacat attcttttat ctctccacca aacatggaac
      541 ggattcccgc accgatttgt ccaccaacac ctctgctgcg gacggtgagt ccataaacaa
      601 atcctttagt atctactatt tcatatcctg gcacataatt tgaactagca aggatgaatt
      661 cctctaccct aaccataaaa atacacctcc taaagtttcc tcaatatatt aataataaat
      721 agttgggggc aataaattta aagttattcc ttccataatg aatatttacc agaatgataa
      781 ttgcaatatt aattagatga tattcgttat ttttctaacc agtttaaatc tttaaattag
      841 taggagagag aagatgaaag tactcatagc tgatcagata aatgaaaagg gaattgatga
      901 gcttaaagac atcgcagatg tggtggttca aaccgatata accccagaag agttagtaag
      961 ttcaatccca gattacgatg ccatagtggt gaggagcagg accaaggtca cccaggaggt
     1021 aatagaagcc agtcagaaac tgaagatcat agcccgggcc ggagtgggtg
//
LOCUS       DAOQVW010000621         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_206956_length_1065_cov_2.752475, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000621 DAOQVW010000000
VERSION     DAOQVW010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206956_length_1065_cov_2.752475"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PKK85_10100"
     CDS             complement(<1..505)
                     /locus_tag="PKK85_10100"
                     /inference="COORDINATES: protein motif:HMM:NF037411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26407.1"
                     /translation="MKALLLRVGIDKGSDGLLAPLFEDRSFEYIPLSERDPDSEEKRT
                     FQNLQGRCGKKLSEYLPEKVVNRKIHHDPEFETFTYADEGRKAQFLIKLEKGDYLVFY
                     AGLASRKKGDGLYLIGYLVVEQVIKLGKLDHDKQHTIQEEHPTNSHLKRHFKEGVVLV
                     VGDKNKSK"
     gene            621..1025
                     /locus_tag="PKK85_10105"
     CDS             621..1025
                     /locus_tag="PKK85_10105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNS26408.1"
                     /translation="MDELVEFFKRDLFAAKNGIETVEIGHGWAEARMELTSEHLNAVG
                     TVHGGALFSLADFAFAMAANSGPQVTVAINVSISYFKAVKSGVLTASAREVSGGGRIA
                     SYTVEIHDEAGDLVALFQGMAYRKRDRLKDLF"
BASE COUNT          282 a          234 c          232 g          317 t
ORIGIN      
        1 atttactctt atttttatcc cccaccacca gcaccactcc ttctttaaaa tggcgtttca
       61 ggtgagagtt agtgggatgc tcttcctgga ttgtatgctg cttatcatgg tccagcttcc
      121 ctaactttat gacctgctcc accaccaggt atccaataag gtacagtcca tcacccttct
      181 ttcgagatgc caaacctgca taaaacacca aataatcgcc tttttctagt ttaattaaga
      241 attgtgcttt acgaccctca tcagcgtatg tgaatgtttc aaattccggg tcgtggtgga
      301 ttttcctgtt aacaaccttt tcagggaggt attccgacaa tttttttcca catcttccct
      361 gtaggttctg gaaggtccgt ttttcctcag aatctggatc cctctcggag agaggaatgt
      421 attcaaagct tctatcttca aatagagggg ctaacaatcc atcactacct ttatctatac
      481 ccacccgtag caacagtgcc ttcattcaac caccttgaaa gtatttaata ctgggtctta
      541 agagaataat aaaatgatcc tataatcagg aagaaattaa tggccaattt gtcaataacc
      601 cagagtccga ggtttttggt atggatgaat tagttgaatt ctttaaaaga gacctttttg
      661 cagcaaaaaa tggtattgaa acagtggaaa tagggcatgg atgggcagaa gccaggatgg
      721 aactcaccag tgaacattta aatgctgtgg gcacggtcca tggaggtgct cttttttctc
      781 tggctgactt tgcctttgcc atggcggcca attcagggcc ccaggtaacg gtggccatca
      841 acgtcagtat atcctacttc aaagctgtaa agtcgggagt tctaacggcc agtgcccgtg
      901 aagtttctgg aggcggcaga atcgccagtt acaccgtgga gatccatgat gaagctgggg
      961 acttggtggc cctgttccag gggatggcct accgtaagag ggataggttg aaggatttat
     1021 tctaaacctt attttgatga tgatcattct aagaagaatt gctat
//
LOCUS       DAOQVW010000622         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_207027_length_1065_cov_2.262376, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000622 DAOQVW010000000
VERSION     DAOQVW010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207027_length_1065_cov_2.262376"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKK85_10110"
     CDS             <1..777
                     /locus_tag="PKK85_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515626.1"
                     /note="FEN-1; Rad2; similar to eukaryotic enzymes;
                     endonuclease that cleave the 5'overhanging flap structure
                     that is generated by displacement synthesis when DNA
                     polymerase encounters the 5'end of a downstream Okazaki
                     fragment; as 5'endo-/exonuclease and
                     5'pseudo-Y-endonuclease activities; cleaves the junction
                     between single and double-stranded regions of flap DNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap structure-specific endonuclease"
                     /protein_id="HNS26409.1"
                     /translation="KGIKPVYVFDGESSHLKKDTLAKRNEVKVQSERRWKDALEEGRI
                     EDARKFAVRTSRMSHDVVNGSKKLLTLMGIPYIQAPGEGEAQASYMVEKGDAWCVGSQ
                     DYDCLLFGAPRMVKNLTISGGLGKLELIQLEKVLEELEITREQLVDLALLVGTDFNPG
                     IRGIGAKKGLKLIKEHGNIFNVLEDRDVIFEVHPEVLQKMFLSHEVSTDYEIVWKSPD
                     REQIVEFLCEGHDFSEERVLNAVDKINKMDSSQSSLEKWF"
     gene            complement(847..>1065)
                     /locus_tag="PKK85_10115"
     CDS             complement(847..>1065)
                     /locus_tag="PKK85_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2119 family protein"
                     /protein_id="HNS26410.1"
                     /translation="TSFFKRDDICMTLEMPCNPVPQSLAVYVNILKIIAGSNSRMELE
                     DKMIQAYPSQVKTAIKYAKEFFGDYPPF"
BASE COUNT          335 a          176 c          266 g          288 t
ORIGIN      
        1 aaggggatta aaccagtgta cgtgttcgat ggagagtcca gtcatctgaa aaaagatacc
       61 cttgccaagc gtaacgaggt taaggtccaa tcagaaaggc gatggaaaga tgccctggaa
      121 gaaggccgta ttgaggatgc ccgtaaattc gcggtaagaa cctcacgaat gtcccatgat
      181 gtagtgaatg gttcaaaaaa gctcctcaca ctcatgggaa ttccatacat ccaagctccg
      241 ggagaaggag aggctcaagc atcttacatg gtggagaaag gtgatgcttg gtgtgtggga
      301 tcacaggatt acgattgtct tctttttggc gcgccacgaa tggtgaagaa tttaaccata
      361 agtggaggat tgggtaagct ggaacttata caactggaaa aagtactgga agaattggaa
      421 ataacccgtg aacagctggt tgatctggca ttactggttg gaactgactt taatcctgga
      481 ataaggggta tcggtgctaa gaagggtttg aaactaataa aagaacatgg gaatattttc
      541 aatgttctag aagataggga tgttatattc gaagtccacc ctgaagtcct gcagaaaatg
      601 ttccttagtc atgaagtaag tactgattat gaaattgtat ggaaaagtcc ggatcgagag
      661 caaattgtgg aattcttatg cgaagggcac gatttttctg aggaaagagt gcttaatgct
      721 gtggataaga taaacaaaat ggactccagc cagagtagtt tggagaagtg gttctaggat
      781 atcggccagt gcattaaaga agtgttccta gattatggat aaaattgaga ttatgtagtt
      841 atcccactaa aaaggtggat aatctccaaa aaattctttt gcgtatttta tcgcagtttt
      901 cacctgcgag ggataggctt gaatcatctt atcttctaac tccattctac tgttagaacc
      961 tgctatgatc tttaatatat tcacataaac tgcgagtgac tgaggaacag ggttacaggg
     1021 catttctaga gtcatgcaga tatcgtccct cttaaaaaaa ctggt
//
LOCUS       DAOQVW010000623         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_207208_length_1064_cov_5.171457, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000623 DAOQVW010000000
VERSION     DAOQVW010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207208_length_1064_cov_5.171457"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..396
                     /locus_tag="PKK85_10120"
     CDS             184..396
                     /locus_tag="PKK85_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26411.1"
                     /translation="MTDTLLYYLFTGLAFTGMILNVKKSYYGFVFWMVSNLAFALQSL
                     FLGVYNMTLLFMIQFLFSVWGLYTWR"
     gene            387..662
                     /locus_tag="PKK85_10125"
     CDS             387..662
                     /locus_tag="PKK85_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26412.1"
                     /translation="MEITIKCEWCGQPLKTRDLRRKYHKVNQDGVFCSLEAQRECNRQ
                     RQQRHTKQNGYKQKTGTGLLGPHRHSDPQVEEYEILKEKRRLKLTIR"
     gene            748..1014
                     /locus_tag="PKK85_10130"
     CDS             748..1014
                     /locus_tag="PKK85_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26413.1"
                     /translation="MGEYKEYNIMEEYERMQKECSKKLEKNIQQSKDNISIIEEDFTC
                     PMCEFMNSTQPPSIELELKRIDTVYGTKNKSKIFQPVNPGDPDT"
BASE COUNT          387 a          226 c          220 g          231 t
ORIGIN      
        1 tgaagaatcg gttgattaag gatggagatt gtggcactgc attgaagata atggtgctga
       61 tggaagagga ggccacagga tgaagttttg tagaaaactc caactaaaca tgaacccaca
      121 ccccgatagg gttacaggct tcgacccctt atgtcccagg cctattgtaa gggggcacct
      181 acaatgaccg acaccctact ttattaccta ttcactggcc tggccttcac cggtatgata
      241 ctcaacgtga aaaagagtta ttatggcttt gtcttctgga tggtcagtaa cctcgccttc
      301 gcattacaaa gcctattcct gggagtgtat aacatgactc tattatttat gatacagttc
      361 cttttctcgg tatgggggtt atacacatgg agataaccat caaatgcgaa tggtgcggcc
      421 agcccctaaa gaccagggac ctacgacgca agtaccacaa agtcaaccaa gacggagtat
      481 tctgcagcct cgaagcccaa cgagaatgca accgacaaag acaacaacga cacacaaaac
      541 agaatggtta taaacagaaa acaggaaccg gactcctcgg accccaccgt cacagtgacc
      601 cccaggtgga ggagtatgaa atacttaaag agaaaagaag actaaaacta accataaggt
      661 gagaagtgat gagagtattc aaattaacat tagaaaactc aaatggagat aaagcccagg
      721 taagaattac caatagctgt agaaggaatg ggagaatata aagaatataa catcatggaa
      781 gaatatgaac gaatgcaaaa agaatgcagc aaaaaactcg aaaaaaacat ccaacaatcc
      841 aaggacaaca tctctataat cgaagaagat tttacctgcc ctatgtgcga attcatgaac
      901 tccactcaac caccaagtat agaactcgaa ttaaaacgga tcgatacagt ctacggtacc
      961 aaaaataaaa gtaaaatatt ccaacctgtg aatccgggtg atcctgatac ttgataaatc
     1021 aaaacccaca tattataaac tgaatagttc tcataggtgg gatg
//
LOCUS       DAOQVW010000624         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_208299_length_1061_cov_2.226640, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000624 DAOQVW010000000
VERSION     DAOQVW010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208299_length_1061_cov_2.226640"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKK85_10135"
     CDS             complement(<1..325)
                     /locus_tag="PKK85_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26414.1"
                     /translation="METLKNNLECADCGQQYPLRDGIPDFHDEIFESPRVEMYQFFDA
                     SAENYESEEHQSVLYNFYGQLNLPDEAISELNLYFQDLLLDLLLPGERVLDVACGTGL
                     FSRGLL"
     gene            complement(387..>1061)
                     /gene="nucS"
                     /locus_tag="PKK85_10140"
     CDS             complement(387..>1061)
                     /gene="nucS"
                     /locus_tag="PKK85_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000014 - single-stranded DNA
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNS26415.1"
                     /translation="ITLIASCKVFYDGRATSRLGMGDRTILVKADGAFIIHQDRNLEP
                     VNWQPSKTKLTVELKNGLVYLKGMRRNPTETLEVEIHRGYQISYYQGEDSQDLELAGY
                     EADMGDLIWQRPELIEPGFRATSREYHTPQGYIDIMGKDREGNLTILELKSRKAGVQA
                     VKQLRRYLDCFSDHKSQVRGVLVAPSATDDALEMLEKQKMEFKAMEAPRELANQKKVT
                     LKDYFK"
BASE COUNT          210 a          309 c          275 g          267 t
ORIGIN      
        1 acagtaaacc ccgggagaaa agtccggtac cgcaggccac gtccagcacc cgctccccag
       61 gcaagagcaa gtccaggagg aggtcctgga agtacaggtt taactctgat atggcctcat
      121 caggcaggtt caactggccg taaaaattat acaaaaccga ttgatgttcc tcggattcgt
      181 agttctcggc ggatgcgtcg aaaaactggt acatctccac ccggggcgac tcaaaaatct
      241 catcatggaa gtcgggtatc ccatccctca agggatactg ctggccacaa tctgcacatt
      301 ccagattatt ctttaatgtt tccaagtcac cgtgacagtt agtgcaccat aaaagtgtcg
      361 tggccctcac ctcttttatt tatctcttac ttgaagtagt ccttcaaagt aaccttcttc
      421 tgattagcta attccctggg tgcctccata gccttgaact ccatcttctg tttctccagc
      481 atctccaggg catcatcggt ggcagatgga gccaccagca ccccacgaac ctggctttta
      541 tggtctgaga aacagtccag gtatcttcgc agttgtttaa ctgcttgcac cccggccttc
      601 cggcttttaa gttccagtat ggtcaggttc ccctcccggt ccttgcccat tatgtcgatg
      661 tacccctggg gggtgtggta ctcccgactg gtggcccgga agcctggttc gatgagttcg
      721 ggccgttgcc agatcaggtc ccccatgtcc gcctcgtagc cggccagctc caggtcctgg
      781 gagtcctccc cctggtagta gctgatctgg tagccccggt gtatctccac ctccagggtc
      841 tcggtggggt tgcggcgcat gcccttcagg tacaccaggc catttttgag ctccaccgtc
      901 agtttggtct tggagggctg ccagttcact ggctccaggt tgcggtcctg gtggatgatg
      961 aaggccccgt cggccttgac gagtatggtg cggtccccca ttcccaggcg gctggtggcc
     1021 cggccatcat agaagacctt gcaggatgcg attaaggtga t
//
LOCUS       DAOQVW010000625         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_208444_length_1061_cov_1.439364, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000625 DAOQVW010000000
VERSION     DAOQVW010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208444_length_1061_cov_1.439364"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>1061
                     /locus_tag="PKK85_10145"
     CDS             192..>1061
                     /locus_tag="PKK85_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2193 family protein"
                     /protein_id="HNS26416.1"
                     /translation="MVELYEKMVKEALMAQRADVDTVKKYRGQEFKIEHTKPYVDVAN
                     KMEAVDGQSESVLNLHKKSINVHFETLKGLTDKVRPEDDPFVEHYQTPTILEILYDED
                     EDFKASMDKFIDAIGKNEALIGREVVRRYGGFYGPTCVVEFALIPGSTSNVVNRILQT
                     VDIPVDHKQAILSSKSWGMNTSYGIGEVFASSIEGGDSLTEAIGKEIEMIKSIYRTPV
                     EAQAKLMDSVGHQSFDVRKYMSQYRNGIEKTVKEAIDDGVHYGNIVTLPAYCVGDIAH
                     HISQSTYNMCKDDV"
BASE COUNT          370 a          185 c          232 g          274 t
ORIGIN      
        1 tcagttcatt tctattacat actaaaggat ttatacaggt tatattccgc attaaatgct
       61 aatatcttga tattttctgt ttttatgcat ttcctgtttt tatagaagaa tttccccgtg
      121 ttaatgagtt ataactataa aaaaggaaca tttatggttt ctcaggtcaa tacattaatg
      181 aggtgtattt tatggtggaa ctatatgaga agatggttaa agaagcttta atggctcaaa
      241 gagcagatgt ggatactgtt aaaaaatata gagggcaaga atttaaaata gaacacacca
      301 aaccctacgt cgatgttgca aacaagatgg aagcagtgga tggtcagagt gaatcggttt
      361 taaaccttca taagaaatcc ataaacgtcc atttcgagac attaaaaggc cttaccgata
      421 aggtcagacc ggaagacgac ccattcgtag aacactacca gactccaact atcctggaaa
      481 tcctgtacga cgaagatgaa gattttaaag caagcatgga caaattcata gatgctatag
      541 gtaaaaatga agcgttgata ggccgtgaag tggtaagaag atacggtggt ttctacggac
      601 cgacctgtgt ggtggaattt gccctgatac caggaagtac cagtaacgtg gtaaacagga
      661 tactacaaac tgtagatatc ccagtcgacc ataaacaggc catactatct tctaaatcat
      721 ggggtatgaa tacttcctat ggaataggag aagtatttgc gagttcaata gaaggaggag
      781 attccctaac cgaagctatc ggaaaggaga tagaaatgat aaagtccatc tacagaacac
      841 cggtggaagc acaggccaag ttgatggatt ccgtgggcca tcaatcattt gacgtcagaa
      901 aatacatgtc acagtacaga aacgggattg agaagacggt taaagaagcg atagatgatg
      961 gagtgcacta cggtaacata gtaaccctac cagcatactg tgtaggagac atagcacacc
     1021 acatatctca gtcaacctac aacatgtgta aagacgatgt t
//
LOCUS       DAOQVW010000626         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_208488_length_1060_cov_4.778109, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000626 DAOQVW010000000
VERSION     DAOQVW010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208488_length_1060_cov_4.778109"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKK85_10150"
     CDS             <1..603
                     /locus_tag="PKK85_10150"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNS26417.1"
                     /translation="LARKRLGFSITHQGVQELNLEVCRALLDQKIPAVPLSPSSFIHT
                     HHKRIEEFETDLIGQYLELGLVPVLYGDVVLDQDLKIKMAVLSGDQILQYLAQQLGAE
                     QVILGTDVDGIYNRNPKTHPNAQLIPLVTSLDDVDFLEGAHTMDVTGGMGGKVKELLQ
                     LAEEEVESQIINLNVEGLLERALMGDKVPGTWIKKRPMKE"
     gene            610..>1060
                     /locus_tag="PKK85_10155"
     CDS             610..>1060
                     /locus_tag="PKK85_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNS26418.1"
                     /translation="MISDRKLEHLRLCLHSDVEYHKKTGLEDVELVHRALPELDLGDI
                     DLSLNLLGKKMEAPLIITAITGGHPQSREVNRKLASAASQHGIGMGLGSQRAGIENPE
                     LESTYTVAREEAPDTLIWGNIGAAQLELAPAAVEMMELDALAVHLNPL"
BASE COUNT          293 a          294 c          295 g          178 t
ORIGIN      
        1 ctggcccgta aaagactggg cttcagcatc acccaccagg gggtgcagga gcttaacctg
       61 gaagtgtgcc gggcccttct agaccagaag atccctgcag tacccttatc accatcatca
      121 ttcattcaca ctcatcataa gagaatagag gaattcgaaa cagacctaat aggacagtac
      181 ctggaattgg gcctggtacc cgtactctac ggggacgtgg tcctggacca ggacttgaag
      241 attaaaatgg cagtgctcag cggagaccag atcctgcagt acctggccca gcagctaggg
      301 gcggagcagg ttatcctggg cactgacgtt gacggaattt acaacaggaa ccccaaaacc
      361 catcccaacg cccagctcat accactggta acctcactgg acgatgtgga cttcctggag
      421 ggagcccaca ccatggacgt caccgggggt atgggtggaa aagtgaaaga actcttacaa
      481 ctagccgagg aagaggtgga gtcccagatt atcaacctta acgtggaggg actcctggaa
      541 agggcgctta tgggagacaa ggtaccgggt acctggataa aaaaaagacc aatgaaggaa
      601 taagaaaata tgatttcaga cagaaaactg gagcacctgc gcttgtgcct ccactccgat
      661 gtggagtacc ataaaaaaac cggactggaa gatgtggaac tggtccaccg ggccctgccc
      721 gagctagacc tgggagacat tgacctctcc ctgaatttgc tgggcaaaaa aatggaagcc
      781 ccactcatca tcaccgccat caccggggga cacccccaat cccgggaggt gaaccggaaa
      841 ctggccagcg ccgcctccca gcacggcata ggcatgggac tgggcagtca acgggctggt
      901 atagaaaacc cggaactaga aagcacctac accgtggccc gggaggaggc accggacacc
      961 ctgatctggg ggaacatcgg agccgcccag ctggaactgg ccccagccgc agtggagatg
     1021 atggaactgg atgccctggc cgtgcacctc aaccccttac
//
LOCUS       DAOQVW010000627         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_210626_length_1054_cov_2.286286, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000627 DAOQVW010000000
VERSION     DAOQVW010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210626_length_1054_cov_2.286286"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKK85_10160"
     CDS             <1..180
                     /locus_tag="PKK85_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26419.1"
                     /translation="VKTSICGQAGSMPSVVEKLVELGITSVSANTDAVATVREVVARV
                     EKKLLLKAARKIIQE"
     gene            267..>1054
                     /gene="mfnA"
                     /locus_tag="PKK85_10165"
     CDS             267..>1054
                     /gene="mfnA"
                     /locus_tag="PKK85_10165"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HNS26420.1"
                     /translation="MEEKGITKDRVFELLRNYKKRDMTHRSGRILGSMCTCPHEVGLK
                     AYHMFLESNLGDPGLFQGTKTMENEVIATLGQLLGENNVYGHIITGGTEANIMAMRAA
                     RNMRRIKDPEIIVPKSAHFSFKKAADILCLKMREAQLDSQYRVDINSVKELISDKTVA
                     VVGIAGTTELGKIDPIEELSDICLEREIFLHVDAAFGGFSIPFLNQVGYDLPKFDFSL
                     PGVCSVTIDPHKMGLAPIPTGGILFRDKSYLDVMSVETPYLTEE"
BASE COUNT          330 a          245 c          247 g          232 t
ORIGIN      
        1 gttaaaacca gtatctgcgg acaggcagga agcatgccct ccgtggtaga gaaactggtg
       61 gaactgggaa tcacctcagt atccgccaat accgatgccg tggcaaccgt aagggaagta
      121 gtggcccgtg tggagaagaa actgctccta aaagccgctc ggaagataat acaggaataa
      181 aatgggataa caaattaatt ttttttaatt tttttacatt ctttttatct acaagctaaa
      241 acatctactt tttaaaggca tacaacatgg aagagaaagg aattaccaag gacagagtat
      301 tcgaattact cagaaactac aaaaaacgag atatgaccca tagatcaggc aggatcctgg
      361 gatccatgtg cacctgcccc catgaagtgg ggctcaaggc ctaccacatg tttctggaat
      421 ccaacctggg agaccccgga cttttccagg gtaccaagac catggaaaac gaagtcatag
      481 ccacactcgg ccaattactg ggggaaaaca atgtctatgg ccatataatc acgggtggaa
      541 ccgaagccaa tataatggcc atgagagccg cccggaacat gcgcagaata aaagatccag
      601 agataatagt ccccaaatca gcgcacttct ccttcaaaaa agcagcagat atactgtgcc
      661 tcaagatgag ggaagcccag cttgatagcc aatatcgtgt ggatatcaac tcggtaaaag
      721 agctgatctc ggataaaacc gtagctgtag ttggtatagc cgggaccaca gagttaggga
      781 agatagaccc catagaagag ctctcagaca tctgcttgga gagggaaata ttcctccatg
      841 tggacgcggc attcggcgga ttttcaatcc ccttcttaaa ccaggtaggt tatgatcttc
      901 ctaaatttga tttctccctc ccgggagttt gttcggtaac catagatccc cataagatgg
      961 gactggcacc catacccacc gggggaatcc tcttccggga taaaagctac ctggacgtca
     1021 tgagcgtgga aaccccctac ttaactgaag agaa
//
LOCUS       DAOQVW010000628         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_210694_length_1054_cov_1.943944, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000628 DAOQVW010000000
VERSION     DAOQVW010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210694_length_1054_cov_1.943944"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..880)
                     /locus_tag="PKK85_10170"
     CDS             complement(170..880)
                     /locus_tag="PKK85_10170"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS26421.1"
                     /translation="MFSNSDLEKMRAEKDIKGLIKALDPEENKHIREQAAYILGDLKI
                     KEAAGPLISVLNDDYWPIRKAAALSLGALGDKQAVPPLISLLNDDYWHVRETAALALG
                     ALRDKRAVEPIIEALKDGSLNVKCEVVDALGVLGDRRASEPLIEILKEDDYILRACTV
                     TSLGKIGDNLSLQFLMNSLEDESYLVRVNAAHALGTIGDESVLPELKKALNKSQGESQ
                     EFERAIRDAMAQITARIA"
BASE COUNT          268 a          250 c          206 g          330 t
ORIGIN      
        1 ggctttatta atatttattg ccctggacct gggtaaaaat agtttgaaac cctattccta
       61 cctggtgacc atatgattgc cctattatcc ctggggtgaa tataaccctg ggctttgtag
      121 tagggctggt ggtagcctat atcctgatat ggaaaaagaa aaaatcaact taagctatac
      181 gtgccgtgat ctgtgccatg gcgtctcgga tggccctttc aaattcctga gattccccct
      241 gggatttatt cagggccttt ttaagttcag ggagaacact ttcatcccca atggtcccca
      301 gggcatgggc ggcgttaacc cttaccagat agctttcatc ttctaaagaa ttcataagga
      361 actgtaatga tagattgtct ccgattttac ccagcgaagt aacggtacaa gcccgcaaaa
      421 tataatcgtc ttccttcaat atttctatta aaggctcgga agctctcctg tcacctaaaa
      481 cccccagggc atccaccact tcgcatttaa catttaaaga accatctttt aaggcctcta
      541 ttatgggttc caccgctcgt ttatccctca gggcacccag ggccagggca gcagtctccc
      601 ttacatgcca gtaatcatca tttaagagtg aaattaaagg cggtaccgcc tgtttatccc
      661 ccaaagcccc taaagataag gcagcggcct tgcggatggg ccagtaatca tcatttagaa
      721 ctgagataag aggtcctgcc gcttctttaa tcttaagatc tcccaggatg taggctgcct
      781 gttcccggat atgcttgttt tcttcaggat ccagtgcctt tataagacct ttaatatcct
      841 tttcggccct cattttttcc agatctgagt tggaaaacat tactccccct ataaaaataa
      901 tataattaag tgtaggattt tacttactta tttctttttt cattatgttt cattctttct
      961 tttacgattt ttttaaccag tccctcagga agcggatttt ctgatgtaaa atgaatggtg
     1021 gtcccggagg tgtggaaatc ccccagttct ttct
//
LOCUS       DAOQVW010000629         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_212078_length_1050_cov_1.862312, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000629 DAOQVW010000000
VERSION     DAOQVW010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212078_length_1050_cov_1.862312"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PKK85_10175"
     CDS             complement(<1..139)
                     /locus_tag="PKK85_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNS26422.1"
                     /translation="MSIIKRREREKRQRENAIINAAEKLFFSKGYDDVSMNDIAREVE
                     LS"
     gene            complement(398..>1050)
                     /locus_tag="PKK85_10180"
     CDS             complement(398..>1050)
                     /locus_tag="PKK85_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-coenzyme M reductase subunit alpha"
                     /protein_id="HNS26423.1"
                     /translation="ATASYTDDILDDFVYYGMEYVDENYGICGTKATTEVVHDIASEV
                     TLYGLEQYEYPALLEDHFGGSQRAAVVSAAAGCSVAFATGNSNAGINGWYLSQILHKE
                     AHSRLGFYGYDLQDQCGASNSLSIRSDEGLIHELRGPNYPNYAMNVGHQPEYAGIAQA
                     PHAARGDAFCANPLIKVAFADSNLTFDFKHPRKSIAKGALREFLPSGERDLISPVG"
BASE COUNT          278 a          249 c          207 g          316 t
ORIGIN      
        1 tgctcagttc tacctccctg gctatatcgt tcatggaaac atcatcatag cctttagaga
       61 agaataactt ttcagcggcg ttgattatag cgttttccct ctgccttttt tccctttccc
      121 ttcttttaat gatggacatg caacttcacc ataacccctt atttttccat cctatccaaa
      181 ttgaattcat catatccccc aatcatggaa cctgttatag aaatataatc aattcatcat
      241 attttaaatc tatcgataat atggttggaa taaaaagtca caaccaccat ttcattagcc
      301 atgcagtcgg taattttgaa aactagttta gaaaaaaatt ctatcctata atttaaaaaa
      361 aaatttggga ttttttcaat ccctgttaaa cttaatccta tccaactgga ctgattaagt
      421 ccctttcccc acttggcagg aactctctaa gcgcaccttt tgcaatggac ttccggggat
      481 gcttgaagtc gaaggtcagg ttactatctg caaaggcaac ttttatgagg gggtttgcac
      541 agaatgcatc tcccctggca gcgtgtggtg cttgggcaat acccgcgtat tctggctggt
      601 ggcccacatt catggcatag ttagggtagt taggaccacg cagttcatgg atcagacctt
      661 catcactcct gatagataag gagttggatg ctccacactg gtcctgcaga tcgtaaccat
      721 agaagccaag tctgctatgt gcttctttgt gtaagatctg acttaggtac catccattga
      781 ttccggcgtt ggaatttcct gtggcgaagg caaccgaaca cccggctgca gcagaaacaa
      841 cggcggctcg ctgtgaacct ccaaagtggt cttccaggag tgccgggtat tcgtactgct
      901 ccagtccata gagggttacc tctgaagcta tgtcatgaac cacttcagta gttgctttgg
      961 tcccgcatat accataattt tcatccacgt attccattcc gtagtagacg aaatcatcca
     1021 gaatatcatc agtgtaggaa gcggttgcat
//
LOCUS       DAOQVW010000630         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_212330_length_1049_cov_2.234406, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000630 DAOQVW010000000
VERSION     DAOQVW010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212330_length_1049_cov_2.234406"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKK85_10185"
     CDS             complement(<1..269)
                     /locus_tag="PKK85_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNS26424.1"
                     /translation="MDSIIEDIRVRKILDSRGNPTLEVDVITWNGFGRAAAPSGASTG
                     AREVIAFPAGGVDKIIGEVEDIISSELIGMDAEDLNEIDLVLKEI"
     gene            complement(370..585)
                     /locus_tag="PKK85_10190"
     CDS             complement(370..585)
                     /locus_tag="PKK85_10190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HNS26425.1"
                     /translation="MARKTSTKKEETPLKLTRFEKARLIGARALQLSMGAKPQVEVSG
                     SIDPIDIATLELKEDVIPLEVKRILKK"
     gene            complement(632..795)
                     /locus_tag="PKK85_10195"
     tRNA            complement(join(632..666,756..795))
                     /locus_tag="PKK85_10195"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(757..759),aa:Gln,seq:ttg)
     gene            complement(800..967)
                     /locus_tag="PKK85_10200"
     CDS             complement(800..967)
                     /locus_tag="PKK85_10200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HNS26426.1"
                     /translation="MIPVRCLSCGKVVSAYFEEYKKRTDEGENPKEVLNDLGIDKYCC
                     RRMFIAHVEVW"
BASE COUNT          253 a          236 c          185 g          375 t
ORIGIN      
        1 tctatctcct tcaaaaccag gtcgatctcg ttcaggtctt cggcatccat ccctataagt
       61 tccgaggata tgatatcctc cacttcacct attatcttgt ccactccccc ggctgggaag
      121 gctatcactt cacgtgctcc ggtgctggct ccgctgggag ctgccgccct tccaaaaccg
      181 ttccaggtta ttacatccac ctctagggtg gggtttcctc tactatctaa gatttttctt
      241 acccgtatgt cttctataat actatccaca ataaacacct ctggaaaaaa tagtggttat
      301 ttcaggcctt atttttggat taactatttt ttttaatgat gaaagtgatc ttccattatt
      361 aaagagtttc tactttttca atatcctctt cacttcaagg ggtattacat cttctttaag
      421 ctctaatgtg gctatgtcta tggggtcaat ggaaccagaa acttctactt gtggtttggc
      481 tcccattgaa agttgaagag ctctggcacc tatgagtcgt gccttttcaa atctggtgag
      541 ttttaaaggg gtttcttcct tttttgtact agtttttctg gccattttct ctctcctatg
      601 gtaaatttca ttgattagat taaaatggaa atggggccgc cgagatttga actcgggtct
      661 ccagcgttcc ggtgttgacc atccccgttt cagtcgaaaa aatatcataa gtttggaaat
      721 ttttttggat ctccttactt aactgaatac ttgtttcaat ggctgagagg atggaccaag
      781 ctaccctacg gcccccatac taccatactt ctacatgggc gatgaacatt ctcctgcagc
      841 agtatttgtc tattcccagg tcattgagga cttccttagg attctcaccc tcatcagttc
      901 tctttttata ttcctcaaaa taggcagata caacttttcc acagcttaaa catctaactg
      961 gaatcatata atcatcgttt attttaaatt tttttttata ttattactta ttgttactga
     1021 gtttttgtgt gaaactacag gtaaaggtt
//
LOCUS       DAOQVW010000631         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_213317_length_1046_cov_2.664985, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000631 DAOQVW010000000
VERSION     DAOQVW010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213317_length_1046_cov_2.664985"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1046
                     /locus_tag="PKK85_10205"
     CDS             <1..>1046
                     /locus_tag="PKK85_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNS26427.1"
                     /translation="CPNRAIFKNKYGGYVVDRAKCNACGVCELSCPVNNITIEDGVVK
                     GICSRCGICVPACPEKARVDAYDVIEDRQLKFLESLNLTVQPPLRSKKGKELVQRTSL
                     VTETEKCTLCRRCEYYCPTGAIQVDVEPHGVCTECRVCEDVCPVGAIENFTIDPDKCT
                     SCLKCIGECPNRAIYTEDFQVKINKLEPGEKLTGKLISCLNCGLCADECPRGALKMVD
                     GRLRYDPNLCEDCENMECLDACPVGILRPSKDPEKRIEGFCVSCGKCVKSCDLQEARS
                     FQQVTWDGSVSDECISCGICAEICPKEAITLRRGTIDVDLDKCVLCEKCAIHCPVQAI
                     PTTTMRRKYIKDGFT"
BASE COUNT          305 a          231 c          260 g          250 t
ORIGIN      
        1 tgtcctaacc gggccatttt caaaaataaa tacggaggat atgtagtgga ccgggccaaa
       61 tgcaacgcct gcggagtttg cgaactctcc tgtccagtaa acaatattac catcgaagac
      121 ggggtagtta aggggatctg ctcccgctgt gggatatgtg tcccagcctg ccctgaaaag
      181 gcacgagttg atgcctatga tgttattgaa gaccgtcaac tcaaattcct ggaatcactt
      241 aatctcactg ttcaaccacc cttaagatca aaaaaaggta aagaactggt gcaaagaacc
      301 agcctagtca cagagactga aaagtgcacc ctctgccgga gatgtgaata ctactgccca
      361 actggagcca tacaggtgga tgtggagccc catggagtat gcactgagtg tcgggtttgt
      421 gaggatgtgt gtccagtggg agccattgaa aacttcacca ttgaccctga taagtgcaca
      481 agctgtttaa agtgtatagg ggaatgtccc aaccgggcca tttacaccga agacttccag
      541 gtaaaaatca acaaattaga acccggggaa aaactcacag gaaaactcat atcatgcctt
      601 aattgcggtc tatgcgctga tgagtgtccc cgaggcgccc tgaagatggt ggatggtcgc
      661 ctgcgctatg atcccaatct ctgtgaggac tgtgaaaaca tggaatgtct ggatgcatgt
      721 ccagttggta ttttaagacc atcaaaagat cctgaaaaga gaattgaagg cttctgcgta
      781 tcttgtggta aatgtgttaa gtcctgtgac ttacaagaag cccgaagctt ccagcaggtt
      841 acctgggatg gatcggtctc agatgaatgt atttcctgtg gaatatgcgc agaaatatgc
      901 cctaaagagg cgataacact acgccgcggt actatagatg tggatctgga taaatgtgtg
      961 ctctgtgaga aatgtgctat acactgcccg gttcaagcca tccccaccac aaccatgcgc
     1021 agaaaatata ttaaagatgg attcac
//
LOCUS       DAOQVW010000632         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_215372_length_1040_cov_3.371574, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000632 DAOQVW010000000
VERSION     DAOQVW010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215372_length_1040_cov_3.371574"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKK85_10210"
     CDS             <1..363
                     /locus_tag="PKK85_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNS26428.1"
                     /translation="VGTTQKKIVNSIRNILSDEDLYRSMKEASNPYGDGTASKKIVDI
                     ISEIESRGELKIEAPDFITGVTTTKLISVDQDINVSKFEDKYRGIVKIVFEDGKIIFP
                     EPGLNLKDKLVLISGTEL"
     gene            554..>1040
                     /locus_tag="PKK85_10215"
     CDS             554..>1040
                     /locus_tag="PKK85_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNS26429.1"
                     /translation="MVCKIREDTKITIYGLGHMGLPTAALLARSGLKILGVDVNPHIV
                     KKINQAQSPILEPGLGEIIHETVKNSRLSASTNIEQASRQSEVHMVIVPTPIDENKAA
                     DLKSVEMACRSIAQGLDKGDLVIIESTVPPGTCQYLATPLLEESGLKVGLDFGLAYTP
                     ER"
BASE COUNT          348 a          184 c          228 g          280 t
ORIGIN      
        1 gtgggaacaa cccagaaaaa aattgtcaac tccattcgga atatcctgag tgatgaagat
       61 ctttatcgta gtatgaaaga ggcttctaat ccatatggtg atggaacagc atcaaaaaag
      121 atcgtggaca tcatatcaga aatagaatct cgtggtgaac tcaaaataga agcaccagac
      181 tttataactg gagttaccac cactaaatta attagtgtgg atcaggacat taatgtatca
      241 aaatttgagg ataaatatcg gggaattgtt aaaatagtat ttgaggatgg aaagataata
      301 tttccagaac cagggcttaa tttaaaggat aaactggttt taatatctgg aacagagctt
      361 tgatcacccc tgtaatttgt gacatagatc aggcaggtct ctttgaatca tttaatggtt
      421 tatagggggt tggaatcatc tttaaataac cttatgtcct taggaatgat tagcccatca
      481 taacaaattc agatctatat ataaatccag attatcacta tcccagacga aaatgagtgt
      541 agaggagatt tcaatggtat gtaagatcag ggaagatacc aaaatcacca tttatggttt
      601 gggccatatg ggactgccca ctgcggcgct cctggctaga agtggcctga aaattttagg
      661 tgttgatgta aatccccaca ttgtaaaaaa gattaaccag gcacaatcac ccatattgga
      721 gccaggcttg ggtgaaataa ttcatgaaac tgttaaaaac tcacgactat ctgccagtac
      781 aaatattgaa caagcttcca gacaatcaga agtgcatatg gttatagtac ccacccctat
      841 tgatgaaaat aaggcggcgg acttgaaatc agttgaaatg gcgtgtagaa gtatagctca
      901 gggtctggat aagggtgacc tggttatcat cgaaagcacg gtgccaccag gcacctgcca
      961 atacctagcc acccccttat tggaagaaag tggattaaag gttggtttag attttggtct
     1021 ggcttacacc cctgagaggg
//
LOCUS       DAOQVW010000633         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_215855_length_1039_cov_2.338415, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000633 DAOQVW010000000
VERSION     DAOQVW010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215855_length_1039_cov_2.338415"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKK85_10220"
     CDS             <1..647
                     /locus_tag="PKK85_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNS26430.1"
                     /translation="PIIAEIDQSKCTMCGKCIDDCKYKAIYMYDERVSVDPIACTGCG
                     MCVSSCEDGAIEIMGQTDSQIFAMISGMLKDKKDGEKIILAFLDYIGYVAADNIGINR
                     ISYPESIRIIRVTSINRLMIKHVLYAFENGADGIFLGEYPDDLVYPDIQEKVHEFKAE
                     LIKNGIDPDRLMYYKVYAPFYRGLAAKFEEFNRQVNKSLDKYEAILSKASSKDI"
     gene            complement(761..>1039)
                     /locus_tag="PKK85_10225"
     CDS             complement(761..>1039)
                     /locus_tag="PKK85_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26431.1"
                     /translation="QHELEHALEDPNKKILAVAYTDTVQNFINQHPEVSSRIVVANAN
                     KNSIQQGLTKLNIAGATSTGFITPLLSGLLIGLVFGLGVGAVWMKRRL"
BASE COUNT          312 a          214 c          231 g          282 t
ORIGIN      
        1 aacccataat agctgaaatt gaccagtcca agtgtaccat gtgcggaaag tgtatagacg
       61 actgtaaata caaggccata tacatgtacg atgaacgtgt atctgttgac ccaatagcct
      121 gtactggatg tggaatgtgc gtctccagct gcgaagatgg tgccattgaa atcatgggcc
      181 agaccgacag ccagatcttc gccatgatct ccggtatgct gaaggataaa aaggacggcg
      241 agaagataat actggccttc ctggactaca taggctatgt tgccgcggat aacattggga
      301 tcaacagaat atcctatcca gaatccatcc gaatcattag ggtcacatcc atcaaccgtt
      361 tgatgattaa acatgtcctc tatgcctttg aaaacggtgc tgacgggata tttttaggag
      421 aatatcccga cgacctggtc tatccagata tccaggagaa agtgcacgag tttaaagctg
      481 agctgattaa aaacggtata gatcccgata ggttgatgta ttataaggtt tatgcacctt
      541 tctaccgggg actggcagcc aagtttgagg aattcaacag acaggtgaat aaatccctgg
      601 ataaatacga ggccatcctg tctaaggctt cttctaagga tatttaagta tttgggaaaa
      661 tttacttaga cccgtaagaa aatctgaggc ttaaaaacca tatacatgga tgaaatactc
      721 cccattaaaa agaagttgag gagattaata gaatcttatt ttacaacctt ctcttcatcc
      781 aaactgcacc aactcccaat ccaaatacca gtccaatgag gagaccagaa agtaaaggag
      841 ttataaagcc tgtgctagtt gcccctgcga tatttaactt ggttaaaccc tgctggatgg
      901 aatttttatt ggcatttgca accacgatcc tgcttgaaac ttcggggtgc tggtttatga
      961 aattttgaac cgtgtctgtg taagccactg ccaagatctt cttattgggg tcttctaggg
     1021 cgtgttccaa ttcgtgttg
//
LOCUS       DAOQVW010000634         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_217173_length_1035_cov_3.533673, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000634 DAOQVW010000000
VERSION     DAOQVW010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217173_length_1035_cov_3.533673"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKK85_10230"
     CDS             complement(<1..591)
                     /locus_tag="PKK85_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNS26432.1"
                     /translation="MKIRRNLLILGIILIIIVLVLGVYLFIPSHIPETSNHAEVVAEN
                     LEVPWAMAFLPDGRMIFTERPGRVSILDGKQVKLVANINATASSESGLLGVAVDPEFN
                     ENHYIYLYYTSNNYNRVSRFELGEKLENETVLIDKIPAAQIHDGGRLKFGPDGKLYIS
                     TGDASQRELAQDTNSLAGKILRMNPNGSVPLDNPFGN"
     gene            737..>1035
                     /locus_tag="PKK85_10235"
     CDS             737..>1035
                     /locus_tag="PKK85_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNS26433.1"
                     /translation="MIIEVKHLLVQEKLSRIRRRDVGRVEFRAGVREIGRLISYQFAN
                     SLEVEDFPVKTPMGTANGVRIKDWDDLIVVSVLRAAIPMVEGIMRVFQEAQSGVAG"
BASE COUNT          287 a          205 c          245 g          298 t
ORIGIN      
        1 gttaccaaag gggttatcta acggtacact gccgttggga ttcatgcgaa gtattttacc
       61 ggctaatgaa ttggtatctt gggccagttc tcgttgcgag gcatcaccag tgctgatgta
      121 tagtttacca tcaggaccga acttcaatcg ccctccgtca tggatctggg cagctggtat
      181 cttatctatc aagacagtct cgttttctaa tttttcacct aattcaaaac gtgaaaccct
      241 attataattg ttggaggtgt agtacagata gatgtaatga ttttcgttga attcaggatc
      301 cacggccaca cctaaaaggc cggattcact gctggctgtg gcgttaatgt tggccactaa
      361 tttaacctgt tttccatcga gaatactgac tctgccgggt ctttcggtga atatcatcct
      421 tccatcaggt aaaaaggcca tagcccaggg cacttccagg ttttctgcca ccacctcggc
      481 atgattactg gtttccggga tgtgacttgg tatgaacaga taaactccca aaacgagtac
      541 tatgatgatt aaaataattc ccaatattag cagatttctc ctgattttca tcttctcccc
      601 ctggaggtta taatatttta ctattctaac atttatcatt ttcaaaacaa aataaatggt
      661 taacaaccac gattcactag taacaaccag gcgtattagt taggatgaat gggttcacga
      721 taaaggggaa tggtacatga tcatagaagt taagcatcta ctggttcagg agaagttgag
      781 caggattaga agacgcgatg ttggacgtgt tgaattcaga gcaggagtga gggaaattgg
      841 gcgcttaatc agctaccaat ttgctaacag cctggaagta gaagattttc cggtgaagac
      901 tcctatggga acagccaatg gagttaggat taaggattgg gacgatttaa ttgtggtcag
      961 tgtactgcga gcagccattc ccatggtgga aggaattatg cgggtatttc aggaggccca
     1021 atccggagtg gcagg
//
LOCUS       DAOQVW010000635         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_217329_length_1035_cov_2.187755, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000635 DAOQVW010000000
VERSION     DAOQVW010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217329_length_1035_cov_2.187755"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1035)
                     /locus_tag="PKK85_10240"
     CDS             complement(<1..>1035)
                     /locus_tag="PKK85_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNS26434.1"
                     /translation="INVEDFHITAASRTLENYLGSYNATVVNRIQDEGGVIVGMTNMD
                     EFAAGSSTETSYFGHTQNPAAEGRIPGGSSGGSAAAIAAGLCDLSIGSDTGGSIRNPA
                     SHCGVMGFKPTYGAVSRQGLLDLAMSLDQIGPFAGDASGIAMMLDTIAGFDERECTTI
                     DWNVPDFTKLTGEGAQDTLKGMKLGVVKQFLDVSDDSIVDIIQGSIDKMEEAGAEVVE
                     LSFDHIDLCLPTYYLINYVEFFSATRKYDGRKYGYKIEDVCGEEVLRRIYIGSYISQK
                     EYSGKYYKKALQARSLIRKEITGLLEGVDSIVGPTVPKLPHKLGTDLEPMEMYAYDVL
                     TVIANLAGIPA"
BASE COUNT          227 a          279 c          234 g          295 t
ORIGIN      
        1 gcgggtatgc cggccaggtt ggctatgacg gtcaggacat cataggcgta catctccatg
       61 ggttccaggt cagtgcctaa tttatgaggt aatttcggta cggtgggccc cactatggaa
      121 tccacacctt ccaggagtcc ggtaatttct ttcctgatga gcgaccttgc ctggagggct
      181 tttttataat atttaccact gtattctttc tgactgatat atgagcctat atatattctt
      241 ctaagcactt cttcgccgca gacatcttct attttatagc cgtattttct tccatcatat
      301 ttacgggtgg ctgagaagaa ctccacatag tttatgagat agtaggtggg caggcataaa
      361 tcgatatgat cgaagcttaa ttccaccact tcggccccgg cctcctccat tttatcgatg
      421 ctcccctgaa ttatatcaac gatggagtca tctgatacat ccaggaactg ttttaccaca
      481 cccagcttca tacctttaag tgtatcctgt gcaccttcac cggtaagctt ggtgaaatcc
      541 ggtacattcc agtctatggt ggtacattcc ctttcatcga accctgctat ggtatcgagc
      601 atcatggcta tgccgctggc atcaccggca aagggaccga tctggtccag gctcatggcc
      661 agatccagga gtccctgcct ggaaacagca ccgtatgtgg gtttaaatcc catcacccca
      721 cagtgtgatg cggggtttct gatggacccc ccggtatctg atcctatgct cagatcacaa
      781 agcccagctg caatcgccgc tgcgcttcca ccgctactcc ccccgggaat acggccttct
      841 gctgctgggt tctgggtatg tccaaagtaa gatgtttctg tggaactccc cgctgcaaac
      901 tcgtccatgt tggtcattcc aactattaca ccaccttcat cctgaatccg gttaacaact
      961 gtggcgttgt agcttcccag ataattttcc agggtccggg aagctgcggt gatgtggaag
     1021 tcctccacgt ttatg
//
LOCUS       DAOQVW010000636         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_217996_length_1033_cov_2.327198, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000636 DAOQVW010000000
VERSION     DAOQVW010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217996_length_1033_cov_2.327198"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..856)
                     /locus_tag="PKK85_10245"
     CDS             complement(23..856)
                     /locus_tag="PKK85_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26435.1"
                     /translation="MPSVDKLPKSKEKGTGEEKKEEGSFFGNLKKSLFGDDLGKQEDL
                     KTSKSPEDVAGESKDKDPVEDRTSVEDKVSTPFPEKKKIKKEEGAFKTEDSVPKTEKT
                     PKTKKGVPQTKESTPQTEKKGTIAETSEEEPTLHIVEDIKSHEIAQWLRKRKEKIIKG
                     VAITISAILILIAIIYSLTPTEQVASNVIFGEGAMFSVFLVLVAFLILAAVFARRLLE
                     GKFLREIHQNLEIVEGKREKDDPKQGDDQKPKSKDDQKKSNGDPSNHDPLTHGMNKKD
                     K"
     gene            complement(944..>1033)
                     /locus_tag="PKK85_10250"
     CDS             complement(944..>1033)
                     /locus_tag="PKK85_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HNS26436.1"
                     /translation="WLQQTRRADESETRFLRCTACGYTWREYD"
BASE COUNT          255 a          216 c          174 g          388 t
ORIGIN      
        1 agattatttt tataaaatat aactatttat cctttttatt cataccatga gttagaggat
       61 catggttgga cggatcacca ttggactttt tttggtcatc ctttgattta ggtttttggt
      121 catccccttg tttgggatca tctttttccc ttttaccttc cactatctca agattctggt
      181 gtatttccct aagaaattta ccctccagta atctacgagc aaagactgca gccagtatca
      241 ggaatgccac caggactagg aatacagaga acatggcccc ctcaccaaag attacgttgg
      301 aagcaacctg ttctgtaggg gttaaactgt aaataatagc tattaggatg aggatagcac
      361 tgatggttat agctactcct tttattatct tttctttcct cttcctaagc cattgagcaa
      421 tttcatgtga ttttatatct tctacgatat gaagagtagg ttcttcttct gaagtttcag
      481 caatggtacc tttcttttca gtttgaggag tgctttcttt agtttgaggc acaccttttt
      541 tagttttagg agttttctca gttttaggaa cactatcttc agttttaaaa gccccttctt
      601 cctttttaat cttctttttt tcaggaaaag gagttgaaac tttatcttca actgaagttc
      661 tatcttcaac cggatcttta tctttagatt ctccagccac gtcttcagga gattttgaag
      721 ttttcagatc ctcctgttta ccaaggtcat ccccgaataa cgattttttt aagttcccaa
      781 aaaaggaacc ttcttccttt ttctcctctc cagtaccctt ttccttggat tttgggagtt
      841 tatcaacgct tggcaaggta agttctcctg tgctcgctaa aatttatctg ttggttaaaa
      901 aatttataac ccttcctatt atttaatctg gtgggtaaaa attttaatca tattcccgcc
      961 aggtgtagcc acatgccgtg caccttaaaa atctggtttc agattcatct gcccttcgag
     1021 tttgctgcag cca
//
LOCUS       DAOQVW010000637         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_219068_length_1030_cov_2.341538, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000637 DAOQVW010000000
VERSION     DAOQVW010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219068_length_1030_cov_2.341538"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1030
                     /locus_tag="PKK85_10255"
     CDS             <1..>1030
                     /locus_tag="PKK85_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNS26437.1"
                     /translation="NRYMSIEKEISYDYLVIATGATSFIPHIPGTTLEGVFKIRTIED
                     GLKIKKWAENSGKAVVVGAGLIGLETAFGLKKMGLEVTVTEMLPQLIPRSLDSDMSKI
                     VQDYIEKEGIRIILGHPLEEIIGKKRVEKAVIGDEVIDTDMVIMATGIRPQIKLAEMA
                     GCEIGRWAILVNEKMQTSVPNIYAVGDCVEVFDAITSQNTQSPLGTTAVRQGKIAAKN
                     IVGVKAEFKAVLNATVSKIGALEFGAVGSSKASAMQNGMEVIAGKSRALTKARYYPGA
                     KRIDVKIISTLKGKIIGCQIIAEERVAERIDTMSLAIARGVTCYELVAMEFSYAPPLP
                     MVIDPIILA"
BASE COUNT          301 a          238 c          269 g          222 t
ORIGIN      
        1 taaccggtac atgagtattg aaaaagaaat atcctacgac tacctggtga tcgccaccgg
       61 agccacttct ttcattcccc acattccggg aaccaccctg gaaggtgttt ttaaaattag
      121 aaccatcgaa gatggcctga aaattaaaaa gtgggcagaa aatagcggaa aagccgtcgt
      181 ggtgggtgcc ggactcatag gcttggaaac agccttcgga ctgaagaaaa tgggtttgga
      241 agtcacggtg acggaaatgc tccctcaact aatccccaga tcactggatt cagacatgtc
      301 caaaatagtc caggactaca tcgaaaagga agggataagg atcatcctcg gccatcccct
      361 agaggagata attggtaaaa aacgggtgga gaaggcggtc ataggtgatg aagttataga
      421 taccgatatg gttataatgg ccaccggtat caggccgcag attaagctgg ctgaaatggc
      481 gggttgtgag attggaagat gggccatcct ggtcaatgaa aaaatgcaaa cctccgtccc
      541 caacatatat gctgtgggag attgtgtgga agtttttgat gccataacca gtcagaacac
      601 ccagtctcca ctgggcacta ccgctgtaag acagggtaaa atagccgcca agaacatcgt
      661 gggagtgaag gcggaattta aagccgtcct caatgccacg gtctccaaga tcggagccct
      721 ggagttcggt gccgttggtt catccaaagc cagtgccatg cagaatggta tggaagtgat
      781 cgccggcaaa agcagagccc taaccaaagc ccgttactat cctggggcga aaaggatcga
      841 cgtaaagatt atatccaccc ttaaaggcaa gattataggc tgccagatca tcgccgagga
      901 aagggttgcc gagaggattg ataccatgtc cctggccata gccaggggtg tcacctgcta
      961 tgaactcgtg gcaatggaat tttcctatgc acctcccctt cccatggtaa tcgatcccat
     1021 catactggct
//
LOCUS       DAOQVW010000638         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_220185_length_1027_cov_2.202675, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000638 DAOQVW010000000
VERSION     DAOQVW010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220185_length_1027_cov_2.202675"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKK85_10260"
     CDS             <1..601
                     /locus_tag="PKK85_10260"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNS26438.1"
                     /translation="AGLRELPGRPMAVKGDDIERIEQKKGLYDDPGEVLSAPFLFDKM
                     DPDAGSDKVRVHVLMLGGGPEIRASTYRVFHQRDHQEIWKHVTDRMAQEIPRLGCTPC
                     IPAIGVGRTHFEASSLMLKAMAYGNLDVQSELEEDITKTLNTLDIGALNIGGYVTALG
                     CLINIGPQRASGVRILSMRPCCCVEPRVSSFDISNREFY"
     gene            749..>1027
                     /locus_tag="PKK85_10265"
     CDS             749..>1027
                     /locus_tag="PKK85_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNS26439.1"
                     /translation="MAIGRKSIEELLKYMNPKWKTSITKVEPNRIITRGYLQQDLIGN
                     LSFPEMVYLLMQGNKPHENESKMLEAVLVSFCDHGTTPPSTQVARLMAS"
BASE COUNT          293 a          218 c          257 g          259 t
ORIGIN      
        1 agccggttta agggagctcc cggggaggcc catggcggtg aagggtgatg atatcgagag
       61 gatcgaacag aagaagggac tctacgatga cccgggagag gtcttatctg ccccgttttt
      121 attcgacaaa atggatccag atgctggcag tgataaagta agggtacacg tcctcatgct
      181 gggcggtggc ccggagataa gggccagtac ctaccgggtc ttccaccagc gggatcacca
      241 ggaaatatgg aagcatgtaa cggaccggat ggcccaggag atccccagat tagggtgcac
      301 cccatgtata cctgccattg gtgtgggcag gacccatttt gaggcgtcgt ctttgatgtt
      361 gaaggccatg gcctatggta acctggatgt gcagtctgag ttggaagagg acataaccaa
      421 aactttaaat actttggata taggtgcttt aaatataggc ggttatgtaa cggcccttgg
      481 ttgtttaatc aacatagggc ctcaaagagc tagtggagtt cgtattctta gtatgagacc
      541 atgttgttgc gttgaaccaa gagtatcatc ctttgatatc tctaacagag aattttatta
      601 gctaagagta tcaatcctta gatactaatg acgagtacat tatgagagta tcaatctttg
      661 atatctttaa ctacaaattt cataagtaag agtatcacct gatatcacta actatgaacc
      721 cgtaaattat attctggagt gaaattttat ggcaattgga agaaaaagca tagaagaatt
      781 gcttaaatac atgaacccta aatggaagac ttccattacc aaggtggagc ccaaccggat
      841 catcaccagg ggctacctgc aacaggacct catcggtaac ttatcctttc cggaaatggt
      901 ataccttttg atgcagggta acaagcccca tgaaaacgag tctaagatgc ttgaagcggt
      961 actggtctct ttctgcgacc acgggacaac tccccccagc acccaggtag ccaggctcat
     1021 ggcttcc
//
LOCUS       DAOQVW010000639         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_220297_length_1027_cov_1.687243, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000639 DAOQVW010000000
VERSION     DAOQVW010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220297_length_1027_cov_1.687243"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKK85_10270"
     CDS             complement(<1..303)
                     /locus_tag="PKK85_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26440.1"
                     /translation="MAINILKPDLIMIAGICAVISFLVTYLAMPRLIKKLNDAKIVGK
                     DIHKPSQPKVAEMGGIGILLGFIIGLFVAIYLYPTLQFTLVITLLVNRPADEITDMR"
     gene            complement(369..818)
                     /locus_tag="PKK85_10275"
     CDS             complement(369..818)
                     /locus_tag="PKK85_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HNS26441.1"
                     /translation="MGYEIHVSKPLTSQKIMELLDKNPELRKITCPPSLYKRIPTRYL
                     DVEVEPIEKLGRPRKYGGKERDMVGKMFKQGYTPQEISNTLNIPLKTVYYLNKTPLKK
                     GRKPKYSPETVRKVKSSHQEGVSAKEISEKLDIPLRSVYSLLKRRYS"
BASE COUNT          233 a          213 c          222 g          359 t
ORIGIN      
        1 ccgcatgtcg gttatttcgt ccgcgggacg gttcaccagc agggtgatta ccagggtgaa
       61 ctgtaaggtg gggtaaaggt agattgccac gaacaaaccg attatgaacc ctaaaaggat
      121 tccgatacca cccatctcag ctactttagg ctggctgggt ttgtggatgt cctttcccac
      181 tatttttgca tcgtttaatt tcttgatcaa ccggggcatg gccaggtagg tgacaagaaa
      241 ggagatgaca gcgcagatac cggcaatcat tattagatct ggtttcagga tgtttatggc
      301 catggtagta ccttcaaatt ttatgaatct ttattctttt tttaaggatg taaatatcgt
      361 cattacgttt atgaatatct tcttttcaag agactataaa cacttcgcag aggtatgtcc
      421 aatttttctg atatttcctt ggcagatacg ccttcttgat gactggactt tacctttctt
      481 acagtttccg gggaatattt aggtttcctc ccctttttta ggggtgtttt attcaggtaa
      541 tacacggttt ttaaggggat attcagggta tttgaaatct cctggggggt gtaaccctgt
      601 ttaaacatct tccctaccat atccctttct tttcccccat attttcgggg tcttcctaat
      661 ttttcgatag gttctacttc cacatccagg taacgggtgg ggatacgttt gtagaggctg
      721 ggggggcagg ttatctttct tagctctgga tttttatcca gcaattccat gatcttctgg
      781 gaagtcagtg gtttgcttac gtggatttca taacccaata ttagcacctg tacatgttta
      841 tactcatatt ataacttttt ttttatccta tttgatcatt tccaggaatt gctgggctct
      901 ggatgatggt ttctggaatg tctctatctt atccctttca gttttcactt cttcaggatt
      961 caggccaaat atctgggcag cttcatccag gtcgatgtcg cccactgaat tcaaaatggc
     1021 agcgccc
//
LOCUS       DAOQVW010000640         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_220411_length_1026_cov_3.486097, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000640 DAOQVW010000000
VERSION     DAOQVW010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220411_length_1026_cov_3.486097"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..>1026)
                     /locus_tag="PKK85_10280"
     CDS             complement(296..>1026)
                     /locus_tag="PKK85_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNS26442.1"
                     /translation="ALVEIFDQLGEEIAAIILEPVMGNVGCIEPKQGYLKFLRKLTQE
                     NDSILIFDEVITGFRLSRGGAQEYYGVTPDLVTLGKILGGGFPMGALAGKKELMEMIT
                     PSGDVYQAGTFNGNPISITAGLTTMELLDNNFYNDLNSSGNQMRSGIQDILDDKSLNY
                     QVAGLVSMFQIYFTEKEVWNYADAKTSDTGKFSRYFHELLREGVFIPPSQFECCFLST
                     AHSPRDINTTLHAIENSITRAESI"
BASE COUNT          298 a          218 c          180 g          330 t
ORIGIN      
        1 attctgtttc tgattcagtt aaaaccacat caaaatctac ttgactaata gcatctttaa
       61 tggcatgatt ttctcctatt ccagctgcca acttcataat tcaccagaaa tagctagttt
      121 acaccaatat tataatatgt tgaatttgta tagaatttct tcttaatatg atttacatca
      181 attcaaattt cccggtattg acaaccctta tcttggatga tgatacccac atttcagact
      241 ggaactcttt ttcaaatatt tttaaacatg gctaagtggt gtagaaaata caaggttaga
      301 tactttcagc ccgtgtaatg gaattctcga tagcatgtaa agtggtgtta atgtcccttg
      361 gtgagtgagc agtgcttaaa aaacagcatt cgaactggga aggtggtata aaaactccct
      421 cccttagaag ttcatggaag tagcgtgaaa atttacctgt gtcggaggtt ttggcatctg
      481 cgtagttcca gacctccttt tcagtaaagt aaatttggaa catagacact aatccggcta
      541 cctggtagtt taaggactta tcgtctaaaa tatcctggat tccactgcgc atttgattcc
      601 cactgctgtt caagtcattg taaaagttat tatctagtaa ttccatggta gtcagaccag
      661 cagtgatgga aatgggattt ccattgaatg tgcctgcctg gtaaacatcc ccagatggtg
      721 ttatcatttc cattaactct tttttaccag ctaaagctcc cattggaaaa cctccaccta
      781 atattttacc caaagtcacc aagtcaggtg tgacgccata atattcctgg gctccacccc
      841 gtgataacct gaatccagtt ataacctcat caaaaatgag tatagaatca ttttcctggg
      901 tgagttttct taaaaatttc aaataaccct gtttcggctc gatacatccc acgttgccca
      961 tgactggctc caatatgatg gctgcaattt cttcccccag ctggtcaaag atttcaacta
     1021 gggctt
//
LOCUS       DAOQVW010000641         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_220425_length_1026_cov_3.242019, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000641 DAOQVW010000000
VERSION     DAOQVW010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220425_length_1026_cov_3.242019"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PKK85_10285"
     CDS             <1..885
                     /locus_tag="PKK85_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNS26443.1"
                     /translation="ETPREDLIRSMKDSLQDMVQCGITTFLDFREGGVDGVEILREAL
                     EDIHVRGLILGRQDSFLDKNADKSKIVSDAQEILKITHGIGLSGFGEIDDETARNIAR
                     ECLKEGKIPAIHVAENEKVQQQSKELTGKTEVERALEAGFKLLVHLTSASTSDLEKLS
                     KQELSVVCCPRANGALSTGIPPIKTMWDLGINLLLGTDNVMINSPNMFSEMEYALKVT
                     RGLTQKYFAPVEILKMATVNVGKAIGLNLGSLEEGYLADIMIVEQISRDPILSLINRT
                     QSKNIIGVITDGKILYQR"
     gene            890..>1026
                     /locus_tag="PKK85_10290"
     CDS             890..>1026
                     /locus_tag="PKK85_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNS26444.1"
                     /translation="MYNKILLPTDGSKHAEKAAEHAIWIASQSGAEIIVLDVIETSSL
                     VG"
BASE COUNT          330 a          197 c          243 g          256 t
ORIGIN      
        1 gaaacaccac gagaagatct cataagatcc atgaaagatt cgctgcaaga catggttcaa
       61 tgtgggatca ccacctttct ggactttaga gagggaggag tggatggtgt tgagatctta
      121 cgggaagctc tggaggatat tcatgttcga ggcctaattt tggggaggca ggattctttc
      181 cttgataaaa atgctgataa gtctaagata gtctctgatg cccaggaaat tttgaagatc
      241 acccatggga ttggtttaag tggctttgga gagatcgatg atgaaactgc ccgaaacatc
      301 gccagagaat gtcttaaaga aggtaagatt ccggccatcc atgtagcgga aaatgaaaaa
      361 gtacagcagc aatccaagga actaactggg aaaacagaag tagaaagggc cttagaagct
      421 ggtttcaaat tactcgtgca tttaacttca gcctccacct ctgatctaga aaaactaagt
      481 aaacaggaac tctcagtagt ttgctgtccc cgagccaatg gcgccctatc cacggggata
      541 ccccccataa aaacaatgtg ggacctggga ataaacctgt tactagggac agataatgtg
      601 atgataaatt ctccaaatat gttcagtgaa atggaatacg cccttaaggt aacaagaggg
      661 cttacccaga agtattttgc cccagtggag attttgaaga tggcaactgt gaacgtaggt
      721 aaggctatcg gtttaaattt aggatcactg gaagagggtt atctggctga cataatgatt
      781 gtagaacaga tctcgcgtga tccgatccta tccctcataa accgtactca atcgaaaaac
      841 ataataggcg taataacaga tggtaaaata ttataccaga ggtgacacta tgtacaacaa
      901 aattttacta cctaccgatg gttctaaaca cgccgaaaag gctgctgaac atgccatatg
      961 gattgccagt caaagcggtg ctgaaatcat tgttcttgat gttatagaaa catcttcact
     1021 ggtggg
//
LOCUS       DAOQVW010000642         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_221646_length_1023_cov_2.434917, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000642 DAOQVW010000000
VERSION     DAOQVW010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221646_length_1023_cov_2.434917"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKK85_10295"
     CDS             <1..604
                     /locus_tag="PKK85_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein A"
                     /protein_id="HNS26445.1"
                     /translation="EGDLIKIENPRVVLRNDHIELSLSRNTPITKADEEETSKIPSIN
                     EIQEKRYPKKKIDEIEENDRNIKVAGKVIEAYGNKILYEMCPNCNKRVNPAGENFVCE
                     ICGEEIEEPNYLMIIPLVIEDETGTIRTTFFRKSAEELIEMTTPEVEEILRKTGDEGS
                     LEDKVNDLLEMEITIIANANYDEYNEEIRLNARKILEKKL"
     gene            647..>1023
                     /locus_tag="PKK85_10300"
     CDS             647..>1023
                     /locus_tag="PKK85_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNS26446.1"
                     /translation="MVELEELPSVGEKTAQKLRDAGFGDMMRLATATAKELSVKADIG
                     EGVAEKVIEAARKAEQIDFETALDVMERRKDVGRITTGSTAVDELIGGGIETQSITEV
                     FGEFGSGKSQISHELAVSVQLPP"
BASE COUNT          381 a          179 c          246 g          217 t
ORIGIN      
        1 tgaaggagac ctaattaaaa tagaaaatcc cagggtggta cttagaaatg accatatcga
       61 gctaagttta agtagaaaca cccccataac caaggcagat gaagaagaaa cttctaaaat
      121 accttccata aacgaaattc aggaaaaaag atatcctaaa aagaaaatcg atgaaataga
      181 agaaaacgat aggaatataa aggtagctgg gaaggtaatc gaggcctatg ggaacaaaat
      241 cctctatgag atgtgtccta actgtaacaa aagggtgaac cctgccgggg agaacttcgt
      301 ctgtgaaatc tgtggagaag aaatagaaga acccaattat cttatgatta tcccgctggt
      361 gattgaagat gaaaccggta ccatacgtac caccttcttc agaaaatcag cagaggaact
      421 cattgaaatg accaccccgg aagtggaaga aatccttcgt aaaactgggg atgaaggatc
      481 tctggaagat aaagtaaatg atctcttgga aatggagata accatcatcg ccaacgcgaa
      541 ttacgatgaa tataatgaag aaataagact caacgcccgt aaaatactgg agaagaagct
      601 ttaatttgaa gatgtgtaaa aaattaaacg ttaaaggaag attaaaatgg ttgaactgga
      661 agaattacca agtgttgggg agaaaacagc ccaaaaactc agagacgccg gtttcggcga
      721 tatgatgagg ttagccaccg ccacagccaa ggaattaagt gtgaaagcag atattggaga
      781 aggagtagct gaaaaagtaa ttgaagctgc ccgtaaggcc gagcagatag actttgaaac
      841 tgccctggat gttatggaac gccggaagga tgtcggccgg ataaccaccg gaagcaccgc
      901 tgttgatgaa ttgatcggag gcggaataga aacccagtca ataactgaag tttttggtga
      961 atttggttct ggtaaaagcc agatatcaca tgaattagcc gtcagtgtac agttaccccc
     1021 gga
//
LOCUS       DAOQVW010000643         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_221980_length_1022_cov_2.962771, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000643 DAOQVW010000000
VERSION     DAOQVW010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221980_length_1022_cov_2.962771"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKK85_10305"
     CDS             complement(<1..337)
                     /locus_tag="PKK85_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNS26447.1"
                     /translation="MKVGILGCGAIANIITNFSQDNKLGVEIAFFYDRDMERAENLAS
                     QVDGRVVLNFEDMLDEVDLVIEAASSQAVEQMVPLILEKGKDVLIMSVGGLLNPQTRK
                     ALESIAKKNN"
     gene            complement(349..669)
                     /locus_tag="PKK85_10310"
     CDS             complement(349..669)
                     /locus_tag="PKK85_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNS26448.1"
                     /translation="MVELTNLYNLDVYTTRGNYVGRIQDVVLNIKKGRVSTLKAVAIN
                     HDKKSVGIKDVITKSIRIVPESDEIRPLKEEGSIDIPYERVQAVGDILLISPEIKEPA
                     VPPQ"
BASE COUNT          292 a          244 c          166 g          320 t
ORIGIN      
        1 cgttgttctt cttagctatt gattcaagag ctttcctagt ttggggattc aaaagtcccc
       61 ccacgctcat gatcagcaca tctttgccct tctctaaaat aaggggaacc atctgttcta
      121 cggcctggga ggaagcagcc tcaataacca gatccacttc atccagcata tcctcaaagt
      181 tcagaacaac ccttccatcc acttgggacg ctaaattctc agccctttcc atatcccgat
      241 cgtaaaaaaa tgcaatttcg acgccgagct tgttgtcttg ggaaaaatta gttattatgt
      301 tagctatggc gccgcatcca agaattccaa ccttcatgtg ctatcttcct attgtggagg
      361 cactgcaggt tcttttatct cgggactgat tagaagtata tctcctacgg cttgcactct
      421 ttcgtaagga atatctatgc taccctcttc tttcagaggc cggatttcat cagattcagg
      481 tactatgcgg atacttttag ttatcacgtc tttgataccc acacttttct tgtcatgatt
      541 tatggcaact gccttcaagg ttgaaactct acctttttta atgttcaaca caacgtcctg
      601 aatccgtccc acgtaattac cgcgggtggt gtacacatcc aagttgtata gattagttaa
      661 ctccaccatc ttaaaccacc tgcctttttt atcattttcc cggtcatatc ctttaatggg
      721 atgccccaga atgttacttt aactaaataa ttttaaaaac ctcaccatac ttaaatattt
      781 cctccgtgcg gtttacaaaa aaccacatct gggtaggtat tcgatcataa aataatcgaa
      841 cctcaccttg tgaaaatatt ataatgcacc caatatataa aattttctta aaaatgtgat
      901 tacagagtca acaatttgcc tatatctcca gcaataagcg gaattactac tgttaaatca
      961 aacccatgat ttttcagtaa aaaatggaag gttaaaatcg tccacggtac ggcagaaatg
     1021 ac
//
LOCUS       DAOQVW010000644         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_222195_length_1022_cov_1.717684, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000644 DAOQVW010000000
VERSION     DAOQVW010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222195_length_1022_cov_1.717684"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1022
                     /locus_tag="PKK85_10315"
     CDS             39..>1022
                     /locus_tag="PKK85_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNS26449.1"
                     /translation="MEIKYVPTICPYCGVGCGMNLVVKDDKVVGVEPWKRHPVNEGKL
                     CPKGNFCYEIIHRDDRLTTPLIKENGEFREASWDEALDLVASKLKAYDPNEIGFFACA
                     RSPNENIYVNQKFARVVVGTQNIDHCARLCHGPTVAGLAASFGSGAMTNSYESFEHAD
                     LIFSIGANSLEAHPLVGRRLMRAKANGAYFIVADPRYTPTAKQSDQYVPFKTGTDVAL
                     MNAMMNVIISEGLEDKEFIEKRTKNYEELKELVSKYTPEMAEEITQVPADVIRDIAIK
                     YAKADKAAIVYSLDITEHSHGVDNVQQTANLAMLTGNIGRLGTGVNPLRGQN"
BASE COUNT          370 a          210 c          225 g          217 t
ORIGIN      
        1 tggaccagaa ctttaaaaca aatgagggga taagaaaaat ggaaattaaa tacgtaccga
       61 caatatgccc atactgtggt gttggatgtg gtatgaacct cgtagttaag gatgataaag
      121 tagtcggggt agaaccatgg aagagacacc cggtaaatga aggaaaattg tgtcctaaag
      181 gaaatttctg ttatgaaatc atccaccgag atgatagatt aaccacacca ctcataaaag
      241 agaatggtga attcagagaa gcttcatggg acgaagcact ggatctggta gcatccaagc
      301 ttaaagcata tgaccctaat gaaataggat tctttgcatg cgccagatca ccaaatgaaa
      361 acatctacgt taaccagaaa ttcgcaagag tggtagtggg aacacaaaac atagaccact
      421 gcgctagact ctgccacggt cctaccgtag ccggacttgc agcatccttt ggatccggag
      481 ctatgactaa ctcttacgag agttttgaac acgcagatct gatattctcc attggagcaa
      541 acagtcttga agcccacccc ctggtgggaa gacggttaat gagagcaaaa gccaatgggg
      601 catactttat agtagccgac ccaagataca ctccaaccgc taaacagtcc gaccaatacg
      661 ttccatttaa aacaggaact gatgtagcac tgatgaatgc tatgatgaac gtgataatca
      721 gtgaaggcct tgaagacaaa gaattcatag aaaaaaggac caaaaactat gaagaactaa
      781 aagaactggt atcaaaatac actcctgaaa tggctgaaga gataactcaa gtaccagcag
      841 atgtaatcag agatatagct ataaaatacg ctaaagctga taaagcagcc atagtttact
      901 cattagatat aaccgaacac tctcacggtg tggacaacgt tcagcaaacc gcgaacctgg
      961 ccatgctcac tggaaacatc ggaagattag gaaccggagt aaacccactg agaggtcaaa
     1021 ac
//
LOCUS       DAOQVW010000645         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_222329_length_1021_cov_2.962733, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000645 DAOQVW010000000
VERSION     DAOQVW010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222329_length_1021_cov_2.962733"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKK85_10320"
     CDS             <1..313
                     /locus_tag="PKK85_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNS26450.1"
                     /translation="DIPRLMDLGPSLVVKTLGKDGSIIYNPEEIRVDALLRESYDPTG
                     AGDSYRAGFLKAFLDGRSLEDCGKFASTVASFIIEAEGCQTNIPTLQMVETRMAKELN
                     G"
     gene            545..>1021
                     /locus_tag="PKK85_10325"
     CDS             545..>1021
                     /locus_tag="PKK85_10325"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNS26451.1"
                     /translation="MTQMDDARKGIITEEMKAVAESENVDPEFIRRSVAKGTIAIPSN
                     KGRKVKAVGIGAGLRTKVNATIGTSTDICDFDMEEEKARVAIENKADTLMELSVGGDL
                     DDIRRRILKISDIPVGSVPAYQAAFEAIREHGATIYMDEDTMFKTIEKQAKDGIDFM"
BASE COUNT          345 a          201 c          237 g          238 t
ORIGIN      
        1 ggacataccg agattaatgg acctgggccc atccttagtg gttaaaaccc tagggaaaga
       61 cggcagtatc atctacaacc ccgaagagat acgggttgat gctctcctaa gggagtccta
      121 cgatcccacc ggggctggtg attcctacag ggccgggttt ttgaaggcct tcctggatgg
      181 tagatcacta gaggattgtg gaaaatttgc ctcaaccgtg gcttccttca taattgaggc
      241 tgaaggatgc cagaccaaca ttcccaccct gcaaatggtt gaaacccgta tggccaagga
      301 attaaatggt taattgcaga aaatgaaata ttaaactgcc atgaggcttt ataagaattt
      361 tctctacggg agaaggataa attaaaaccg taaaccatac ctatttttat ggtaaacatt
      421 aaattagatc agggaaatta tatcagctta cgctctataa tgctgtttat taatctatag
      481 aaactaaaag ttaaagaaac tagaattcga ctaatttata cagctaaaac gcatggtgat
      541 ttctatgaca caaatggatg atgcaagaaa gggcataata accgaagaaa tgaaggcggt
      601 cgcagaaagt gaaaatgttg acccagaatt catcagaaga tccgtggcta aaggaaccat
      661 agccatcccc agtaacaaag gaagaaaagt gaaagcagta ggtatcggag ccggcctaag
      721 aaccaaggta aacgccacca tcgggacctc cactgacatc tgtgactttg acatggaaga
      781 agaaaaggct agagtagcca tagaaaataa agccgatact ttaatggagc tctctgtggg
      841 tggagacttg gatgacatcc gtagaagaat tttgaaaata tccgatattc cagtggggag
      901 tgttccagcc taccaagcag cttttgaggc cattcgggag cacggagcca ccatttacat
      961 ggacgaagac accatgttta aaaccatcga aaaacaggca aaagatggta ttgacttcat
     1021 g
//
LOCUS       DAOQVW010000646         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_223247_length_1019_cov_2.020747, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000646 DAOQVW010000000
VERSION     DAOQVW010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223247_length_1019_cov_2.020747"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..>1019
                     /locus_tag="PKK85_10330"
     CDS             101..>1019
                     /locus_tag="PKK85_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNS26452.1"
                     /translation="MADYIIETQNITRKFDGFKAVDGLSLQIKKGEVFGFLGPNGAGK
                     TTSINMMVGLLKPTSGWVIIDGQDMEKFDKAAIGVCPQEIVLWDNLTCAESLKLIGDM
                     YEVPKDVLKQRVDQLLEDLFLTDKANTLVKNLSGGMKRRLNLAMAVVHQPEIVVLDEP
                     SEGLDPQSRQVLWKYILSLRDEGKTVILTTHLMDEADRLSDRVAIIDHGKLLKLDTPV
                     NLKKEIGGGDVVEITLSDDSMNQKVIKKIEETGNVLSVLEVEGIINLRALNAVGKLPQ
                     IIDDIEETGARMADLSVRQNTLEDVFIDLT"
BASE COUNT          321 a          198 c          259 g          241 t
ORIGIN      
        1 taaaactggc aattcccatc atagaacaaa atataatagc agtgatcatc caataaaatt
       61 aagaatctaa aatttggagt aagcaatacg gcggatgaat atggctgatt acatcataga
      121 aactcagaat atcaccagga aattcgatgg ctttaaggca gtggatggac tgagtttaca
      181 gataaagaaa ggggaggttt ttggtttttt aggtcccaat ggagccggca aaaccacctc
      241 tatcaatatg atggtggggt tactgaaacc caccagcgga tgggtaataa tcgacggcca
      301 ggatatggag aagttcgaca aggcagctat tggtgtttgt ccccaggaaa tagttctatg
      361 ggacaatctc acctgtgctg agagccttaa actaataggg gatatgtacg aagttcccaa
      421 ggatgtttta aaacagagag ttgaccagct actggaggat ctcttcctta cagacaaggc
      481 aaataccctg gttaagaatc tgtccggggg aatgaagcgc cgtttgaatc tggccatggc
      541 agtggttcac cagccggaaa tagtggtgct ggacgaacca tcagaggggc tggatcccca
      601 gtcacgccag gtgctctgga aatacattct ctctttacgt gatgaaggaa aaacagtcat
      661 ccttaccacc catcttatgg atgaggcaga tagactgtcc gatagggtgg ctataattga
      721 ccacggtaaa ctccttaaac tggacactcc ggtgaacctt aaaaaggaaa ttggaggggg
      781 agacgtagtg gagataaccc tttctgatga cagcatgaac cagaaagtaa ttaaaaagat
      841 tgaagagacg ggaaatgtat tatccgtcct ggaagtagag ggaattatta acctccgggc
      901 attaaatgcc gtgggtaagc ttccacagat catagatgat attgaagaga caggagcgcg
      961 tatggccgac ctttcagtac gccagaatac cctggaagac gtgttcatcg atctcactg
//
LOCUS       DAOQVW010000647         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_223267_length_1019_cov_1.933610, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000647 DAOQVW010000000
VERSION     DAOQVW010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223267_length_1019_cov_1.933610"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKK85_10335"
     CDS             <1..385
                     /locus_tag="PKK85_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HNS26453.1"
                     /translation="WIDPDQSEEVSQTITREGVKKLIADGTIKARPKKGISSYRSKKI
                     AQQKSKGKRKGRGSIKGAKGARNPKKKAWMTTIRALRTDLKDMRDNREINRTTYRKLY
                     KMAKGGAFRSKSYMKTYARDHDLLR"
     gene            399..>1019
                     /locus_tag="PKK85_10340"
     CDS             399..>1019
                     /locus_tag="PKK85_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016358838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNS26454.1"
                     /translation="MAQGSRYKVAFKRRREGKTNYGARLKLISLDQCRLVVRLSSQHT
                     LVQIIKVAPEGDHTLVSAHSKEIKDLGWLGSGKNTSSAYLTGYLCGKKALKEGIEGAV
                     LDLGLKTSINGSKIYAALKGAVDAGMNIPHNESILPPEERIRGEHVAAYAQSLDKEEM
                     DLPDPDKIQPPVQLLSINYNFLNSEYFKLIDPKAQQALMTLNEKVMA"
BASE COUNT          333 a          256 c          267 g          163 t
ORIGIN      
        1 atggatcgac ccggaccaat cagaagaagt gtcccaaacc attacccgtg aaggagttaa
       61 gaaactcata gccgacggca ccataaaagc ccggcccaag aaggggatca gcagttaccg
      121 ctccaagaag atagcacaac aaaaaagtaa aggaaaaaga aaaggccggg gaagtatcaa
      181 aggtgctaaa ggcgccagaa accccaagaa gaaggcctgg atgaccacca tcagggcgct
      241 gagaacggac ctgaaggaca tgagggataa ccgggagatc aaccggacca cctaccgtaa
      301 actgtacaag atggccaagg gcggagcatt ccgcagtaaa tcctacatga aaacctacgc
      361 ccgggaccac gacctactaa gataatgggg gaatgcaagt ggcacaagga tcaagatata
      421 aagtagcatt caagagaaga agagaaggaa aaactaatta cggggccagg ttaaagctca
      481 tcagcctaga ccagtgtcga ctggtggtta ggttaagcag ccagcacacc ctggtccaga
      541 tcatcaaggt agcccctgaa ggagatcaca ccctggtatc cgcccattcc aaggaaatca
      601 aggacctggg ttggttgggc agtggaaaaa acacctcctc cgcctacctc acaggatacc
      661 tgtgcggtaa aaaggcactg aaggagggta tagagggggc cgtcctggat ttgggactaa
      721 aaacctccat caacggctct aaaatctacg ccgcccttaa aggagcagtg gacgctggga
      781 tgaacattcc ccacaacgag tccatcctac cccctgagga gaggataaga ggagaacacg
      841 tggcagccta cgcccaatcc ctggacaaag aggagatgga tttgcccgat ccggacaaga
      901 ttcagccccc ggtgcagctg ctgtcgatca attacaactt ccttaattcc gagtatttca
      961 aactgatcga tcccaaggcg caacaggcgt tgatgacact caatgaaaag gtaatggcg
//
LOCUS       DAOQVW010000648         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_223426_length_1018_cov_3.299065, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000648 DAOQVW010000000
VERSION     DAOQVW010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223426_length_1018_cov_3.299065"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="PKK85_10345"
     CDS             <1..93
                     /locus_tag="PKK85_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNS26455.1"
                     /translation="EKAEAKFENGVLRVEIPKQEKDEGISIEIQ"
     gene            150..647
                     /locus_tag="PKK85_10350"
     CDS             150..647
                     /locus_tag="PKK85_10350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNS26456.1"
                     /translation="MIRLDKQTQDLIEKLIKQFEDKDPQVRFEAAKKLGEVGEEAIDP
                     LLELLKSEDDKLRKYATVALKTMGSEKVVDNLILALKDEDFGVRKYSAKALGELKSSE
                     AVDPLLETLEDEDWGVRLAVMKALGDIGDEKAIDPIKKARRKATGDKDFKKVANKALK
                     KIQKK"
     gene            complement(813..>1018)
                     /locus_tag="PKK85_10355"
     CDS             complement(813..>1018)
                     /locus_tag="PKK85_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNS26457.1"
                     /translation="ARDEKASYVAYGHSLVADPWGSVVAEAGTGEEIIYAPIKTSHLR
                     KARQDLPLLKNRRTDLYSLGLKK"
BASE COUNT          303 a          201 c          274 g          240 t
ORIGIN      
        1 gaaaaagctg aggccaaatt tgaaaatgga gtgctcaggg tagagatacc taaacaggaa
       61 aaagatgaag gaatcagcat cgaaatccag tagtactgga tattacatct ttttttcttt
      121 taaggaacaa ttactgtaga gtagtggaag tgattcgctt ggataagcaa acccaagatc
      181 tcattgaaaa attaataaaa cagttcgaag acaaagaccc ccaagtacga tttgaggccg
      241 ccaagaagct gggcgaggta ggggaagagg ccatagaccc cctgttggag cttttgaaat
      301 ctgaagatga taagctgcgt aaatatgcca ccgtagccct gaagaccatg ggctctgaga
      361 aggtggtgga taacctgatc ctggcactga aagatgaaga ttttggggtg cgcaagtact
      421 cggccaaggc cctgggtgag cttaaaagca gtgaagctgt ggaccccctc ctggagacat
      481 tggaagatga ggattgggga gttcgcttgg cggtgatgaa ggccctaggc gatattggtg
      541 atgaaaaggc aattgacccc attaaaaagg ccaggagaaa ggccactggg gacaaagact
      601 ttaagaaggt agctaacaag gccctgaaga agatacagaa aaaatagtcc cgagatggct
      661 ggtgcatggg tgcccacgcc catcattgat tccctgaaga ccattttgaa gttaatcccc
      721 ctcaggtgcc gtgaattgtt aataggggga gtggctggat ttaaggtgga tccggtttta
      781 tttcttatta taaaaaaaaa attgtgaagt gtctactttt tcaagccaag actgtagaga
      841 tccgtgcgcc tgttttttaa taggggcaga tcctgacgtg cctttctaag atgggaagtt
      901 tttatcgggg catagattat ctcctcccca gttccggcct cggccaccac agatccccag
      961 ggatcagcca ccagtgaatg tccataggca acgtatgagg ctttctcatc acgtgcag
//
LOCUS       DAOQVW010000649         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_224479_length_1015_cov_4.227083, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000649 DAOQVW010000000
VERSION     DAOQVW010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224479_length_1015_cov_4.227083"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKK85_10360"
     CDS             <1..396
                     /locus_tag="PKK85_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF108 domain-containing protein"
                     /protein_id="HNS26458.1"
                     /translation="GIDGIKAASIGKIQKAVLITRKPPHSLGIETHKETILYHGTASA
                     AVEKFPLNINVAATLSLAAGMEIEVQIIADPQVDRNMHEVRVVGDFGEFRTVTKNVRC
                     SINPKTSVMAAYSAIKLLNSLNENLQVGT"
     gene            463..>1015
                     /locus_tag="PKK85_10365"
     CDS             463..>1015
                     /locus_tag="PKK85_10365"
                     /inference="COORDINATES: protein motif:HMM:NF016341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HNS26459.1"
                     /translation="MKECEIYANLRVPCSSKVVVRADGRNFQRLTRELELEKPYDEVF
                     AKLMASVGTDIFHEFSPVMVYIFSDEINILLGEIPFSGRVEKIDSVFAGFISSSFTQK
                     LLKNQDPPQLSRPPSFDSRIIPLSPEGVVKYFQERQNEAWRNCLNGYAYWTLRKEYSY
                     QKVIEILRRLRSKDLHDLLFSRGI"
BASE COUNT          300 a          219 c          225 g          271 t
ORIGIN      
        1 ggtatagatg gtattaaagc agcatcaatt ggtaaaatcc aaaaggcagt ccttataacc
       61 cgtaaacctc cccattcact cggaattgaa acccataaag aaaccatact ttaccatgga
      121 acggccagtg ctgcggtgga gaagtttccc ctgaacatta acgtggctgc tacgttgagt
      181 ctagctgctg gtatggaaat tgaggtgcaa ataatcgctg acccccaagt tgatcgtaac
      241 atgcacgagg tccgggttgt aggtgacttc ggtgaattcc gaactgtaac caaaaacgtt
      301 cgctgctcaa ttaaccctaa aaccagtgtt atggccgcat attctgccat taaactctta
      361 aatagtctta acgaaaatct acaggtaggt acttgaaatc ctaatttttc ccacatcaca
      421 atcctcgatg tttccagata gatgtaccag tggatgttca agatgaaaga atgtgaaatt
      481 tatgccaatt tgagagttcc ctgcagttct aaggtggtgg taagagcaga cgggagaaac
      541 tttcaaagac tgacccggga actggaatta gaaaaacctt acgatgaagt ctttgccaag
      601 ttaatggcta gtgtgggaac tgatattttc catgaattca gcccagttat ggtgtacatc
      661 ttctctgatg agatcaacat tctcctggga gagatcccct tctcgggacg ggtggagaag
      721 attgattccg tttttgctgg ttttataagt agtagtttta cccagaaact cttgaaaaac
      781 caggaccctc ctcagttatc ccggccccca tcctttgatt cccgcatcat acccctttct
      841 ccagaggggg ttgtaaagta cttccaggaa cggcaaaatg aagcctggag aaactgtctt
      901 aatggctatg cttactggac actgagaaag gagtactcct accaaaaggt gattgaaatc
      961 ctcagaagac taagaagcaa ggacctacac gatttacttt tcagtagggg gatta
//
LOCUS       DAOQVW010000650         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_224866_length_1014_cov_3.605839, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000650 DAOQVW010000000
VERSION     DAOQVW010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224866_length_1014_cov_3.605839"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKK85_10370"
     CDS             complement(<1..820)
                     /locus_tag="PKK85_10370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNS26460.1"
                     /translation="MSWIILIFILILASVKNRNKKRELTVSVIIPAFNEEKTVGEVVK
                     VVKSLNYIYEIIVVDDGSTDNTGEVAKSAGAMVISHIKNRGKGAAIKTGFNNSKGSVA
                     VFLDADIQNLTAAKVDKIIQPILINEADVTKTKFKREAGRVTELTAKPLLNFFFPEIK
                     FEQPLSGQFAAKRSFLKDIKLEDDYGVDVGIVLDADVRGMRIKEVDIGKIDHVLSSLH
                     ELNIVATEVVRTIVDRATEYGRITMIDTLGKHIRMGLLGLSLATLGFFCVFFIRF"
BASE COUNT          300 a          205 c          181 g          328 t
ORIGIN      
        1 agaatcttat gaagaataca cagaaaaatc ctagggtagc cagtgaaagg ccgaggagtc
       61 ccattcgtat atgttttccc agagtgtcaa tcatggtaat ccgaccatac tctgtggcgc
      121 ggtctacaat ggttcttaca acctctgtag ccacgatgtt caactcatgg agggaggaaa
      181 gtacatggtc gatttttcca atgtcaactt ctttgatcct catcccacgg acgtctgcat
      241 ctagaactat acccacatca actccataat cgtcttctaa ttttatatcc tttaaaaaag
      301 accttttagc agcaaactgg ccactgagtg gttgttcaaa cttgatctcg gggaagaaaa
      361 aatttaggag gggtttagcc gtaagttcag ttacccgccc tgcttcccgt ttaaatttgg
      421 ttttggtaac atctgcctcg tttatgagga tgggttggat gattttatcc actttagctg
      481 cagttaaatt ttggatatcc gcgtctaaaa ataccgctac actgcctttt gaattattaa
      541 aacccgtttt tatggctgct cctttcccac ggttttttat gtgactgatg accatggcac
      601 cagcggattt ggcaacttct ccggtgttat cagtggaacc atcatccact actattattt
      661 catagatata attcagggat ttaaccactt taactacttc accaacggtt ttttcctcgt
      721 taaatgcggg tataataact gaaacagtca gttctctttt tttattccta tttttaactg
      781 acgccaaaat taagatgaaa atgagtatga tccaagacac ttagtcacac ctcttaagtt
      841 caattacgtc tatcttttta tatataaatt gatgtggagt attaaataat gaaggttcta
      901 caaaacttta ttaccaattt aacataaagt tttagtgaat aacaagcaaa tagcatctga
      961 aaaatcagtt taaacctaac tatggaattg gggattacca tggaacctaa agta
//
LOCUS       DAOQVW010000651         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_226789_length_1010_cov_1.435602, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000651 DAOQVW010000000
VERSION     DAOQVW010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226789_length_1010_cov_1.435602"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..562)
                     /locus_tag="PKK85_10375"
     CDS             complement(224..562)
                     /locus_tag="PKK85_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26461.1"
                     /translation="MAKKDKSVLVKDELQSRLKQFREALQDENRRIELENGILGSEVL
                     IRFEVFFQSPKTGNYSDGLFLYMNDSGEIVDAEYYIRDADDVTIAGLETEDFNVVKEL
                     FKDAFSLEIE"
     gene            complement(782..>1010)
                     /locus_tag="PKK85_10380"
     CDS             complement(782..>1010)
                     /locus_tag="PKK85_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26462.1"
                     /translation="VACLPEHDGLDPQRIATAVKEVGARRCILATDLGQIHNPQPTQG
                     FKVFIDTMMELGISHKEVEIMCRVNPNHLFY"
BASE COUNT          321 a          204 c          155 g          330 t
ORIGIN      
        1 aaagacaact tataataatc ggacgggttg tttatttaag taaatgattt tttggacctt
       61 tctactatgt aaatcatatt tttagcatat aattctatgg attactacct atattaatct
      121 ttacaaatag gagagatagg ttattaaaaa aaattaaaaa ttatttaaaa tttaaatgta
      181 actcctaaaa gatgtaatat ttaaaagaaa aaaatatggg aatttattca atttccaggg
      241 agaaagcatc tttaaacagt tccttaacca cgttgaaatc ctctgtctcc aggccggcta
      301 tagttacatc atctgcatcc ctaatgtaat attcggcatc cactatctca ccggaatcat
      361 tcatgtacag gaaaagacca tcagaataat ttcctgtctt cggtgactgg aagaaaactt
      421 caaacctgat tagaacctct gatcccagaa taccgttttc aagctcgatc ctcctgtttt
      481 catcctggag agcttcccta aactgtttca accttgattg aagttcatct ttaactaata
      541 cagacttatc cttttttgcc ataataatac accattaaat aattaacctt cgggtcttaa
      601 tttcctatac gttcctataa tatgatgttg cttttaaaga ttatcctaat ccatgttcac
      661 accgaacttt atgtaaatta aatttttcct tcaataaata gttaccgaat gagtttcctc
      721 caataaaaag ttgctgagtg ggaaactcta gtaaaactcc ctggtagggt cctggtttac
      781 gttaataaaa taaatgatta ggattcaccc gacacataat ctctacctct ttatgggaaa
      841 ttcctagttc catcatggta tcgataaaca ctttaaaacc ctgagtgggt tggggattgt
      901 gtatctgacc cagatccgtg gccagtatac atctacgggc acccacctcc ttaaccgctg
      961 tggctatcct ctgtgggtct aagccatcat gttctggcag gcaagccaca
//
LOCUS       DAOQVW010000652         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_227083_length_1009_cov_1.930818, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000652 DAOQVW010000000
VERSION     DAOQVW010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227083_length_1009_cov_1.930818"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..>1009
                     /locus_tag="PKK85_10385"
     CDS             115..>1009
                     /locus_tag="PKK85_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNS26463.1"
                     /translation="MKDEIAVIIGAGPAGLTAAYQLLDKTTIKPVIFEATGDIGGISK
                     TVEFNGNRMDIGGHRFFSKSEQIMDWWKNILPLQGAPARDDILLDREIPLSESSSIKP
                     IGNRESRTYPSPDPEKTDKVMLNRNRVSRILFLRKFFDYPISLNLKTLSNLGLVKTIK
                     IGLSYIKTSIKPVRDEKSLEDFFINRFGRELYETFFKDYTEKVWGVPCSEIKADWGAQ
                     RIKGLSISKAIAHSIKNIYQKDRSTSQKKVETSLIGQFMYPKYGPGQMWEEVARQITG
                     NGGEIHRNHEVVELSREEDRIT"
BASE COUNT          338 a          210 c          218 g          243 t
ORIGIN      
        1 ttacagcaga aataggctta ttctacctat tatccaaaat tttagcagcc ataatcattt
       61 tgttctggaa tttcttcgcc cgaaaattaa ctctatttag ataggtaaaa aactatgaaa
      121 gatgaaatag cggtcatcat aggtgctggc ccggcgggtc taacagcagc ttaccaatta
      181 ctggataaga ccaccataaa accagtgata tttgaggcaa ctggtgatat tggtggtatt
      241 tctaaaaccg ttgaatttaa tggcaaccgc atggatattg gtgggcatag attcttctcc
      301 aaatcagaac agatcatgga ctggtggaaa aacatcctac ccctccaggg agcccccgcc
      361 agggatgaca tcctcttgga ccgtgaaatt cccctatctg aaagttcaag tataaaacca
      421 atagggaacc gtgaaagtag aacatacccc tctcctgacc cggaaaaaac tgataaagtt
      481 atgctgaacc ggaacagggt gtccagaata cttttcctgc gtaaattttt tgactaccct
      541 atatccttga acttgaaaac attgtccaac ctgggactgg ttaaaaccat caaaataggg
      601 ttaagctata ttaaaacatc cataaaacct gtaagagatg aaaaatccct ggaagacttc
      661 tttataaaca ggttcggccg ggaactctac gaaacattct ttaaagatta cacagagaag
      721 gtctggggag ttccatgcag tgagatcaag gcagattggg gtgcccagag aattaaagga
      781 ctgtccattt caaaagccat agcccacagt ataaaaaaca tttaccagaa ggatagatcc
      841 acatcacaga agaaggtgga gaccagcctc attggacagt tcatgtatcc taaatacggt
      901 cccggacaga tgtgggaaga agtcgcccgg caaataaccg gtaacggtgg tgaaatacac
      961 cgcaaccatg aagtggtgga gttatccagg gaagaagata ggataactg
//
LOCUS       DAOQVW010000653         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_227525_length_1008_cov_1.609654, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000653 DAOQVW010000000
VERSION     DAOQVW010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227525_length_1008_cov_1.609654"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKK85_10390"
     CDS             <1..214
                     /locus_tag="PKK85_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNS26464.1"
                     /translation="LILDKYELGTSVEKVMVKDVAVIGPDEDLIKAVKKMRSSSDGII
                     NQLVVMEGNKMTGIISDGDIIKALKF"
     gene            complement(405..>1008)
                     /locus_tag="PKK85_10395"
     CDS             complement(405..>1008)
                     /locus_tag="PKK85_10395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNS26465.1"
                     /translation="NLLLKEIHHRVKNNLQIVSSLLSLQSQYVNEPGSVELFKESQSR
                     IISMALIHEKLYQTGDLTRINLSEYANELIGDLFRSYSVNTHLVKYQIESKNILLDIN
                     TAIPCGLIINELVTNSIKHAFPDDRSGEIKIDIQCRDDNCILKIADNGVGFPDDLDIN
                     DVKTLGLQLVTSLTKQLDGTIELDREGGTCFTIKFNEPGC"
BASE COUNT          269 a          203 c          219 g          317 t
ORIGIN      
        1 ccttatactg gataaatatg aactgggaac cagcgtggaa aaagtcatgg tgaaagacgt
       61 ggcagtcatc ggtcctgatg aagatctaat aaaggcagta aaaaagatga gatccagttc
      121 ggacggaata ataaaccagc tggtggtaat ggaaggaaat aagatgactg gaatcatatc
      181 cgatggggat ataataaagg ccctcaaatt ttaaaaatag taccctttct acacgcaggg
      241 ctttactcgt tattttttac acctgggctt ttaaattctt acttttttat tcaacacttg
      301 ggcattaaat ctttcaaatc agggccctta aatcattact tttttaacac ccagggcttt
      361 aatttttaat tttttacacc gggctttaaa tttgttactt tttctcaaca cccgggctcg
      421 ttgaatttaa tggtaaaaca ggtgccgcct tctctatcga gctctatggt accatccagt
      481 tgtttggtga ggctggtaac cagttgcagg cccaatgtct ttacatcatt tatatccaga
      541 tcatctggga aaccaacacc gttatctgct atctttaata tgcagttatc gtcccggcac
      601 tggatgtcaa tcttaatttc cccactccgg tcatcgggga aggcgtgttt aatagaattg
      661 gtcaccagtt cgttgattat cagtccgcag ggaatggcgg tgtttatgtc caggaggatg
      721 ttcttggatt ctatctgata cttcaccagg tgggtgttga cactgtagga ccgaaacagg
      781 tcgccgatca gttcatttgc atattcagag aggtttatcc tggttaaatc cccggtttgg
      841 tacagttttt catgtattaa ggccatggat attatcctgc tctggctttc cttgaataac
      901 tcaacagaac caggttcatt cacatactgg gactgtaaac ttagaagact ggatacgatc
      961 tgcaggttgt tcttaacccg gtggtgtatc tccttgagca ggaggttc
//
LOCUS       DAOQVW010000654         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_227627_length_1007_cov_3.409664, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000654 DAOQVW010000000
VERSION     DAOQVW010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227627_length_1007_cov_3.409664"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..948
                     /locus_tag="PKK85_10400"
     CDS             115..948
                     /locus_tag="PKK85_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS26466.1"
                     /translation="MEIVCLIDNHLEDSRLASEHGLSVYIDQGKEKILFDTGATGKAV
                     ENAMTMGVELEKVELAIISHGHYDHGGGLEAFLEVNRKAQVYMCQGAAKKHFRIDNDV
                     VKDIGLDESVLKKYSERITYLDEFIQLRDDLFLITSIRQVHAVPEGNKFLYEEEGLEL
                     VQDSFTHELVMVLKREDGLVVLTGCSHNGILNILDAVVEKFPETPLIAVFGGLHLMIP
                     PDDSVVDPRELDIMARKLMEYPIGRIYTGHCTGIRSYQFLKRIMGDRINYFATGSRVT
                     I"
BASE COUNT          305 a          195 c          250 g          257 t
ORIGIN      
        1 ccaggatgaa agactagtta tattccgggt gtcagaggga gaggcccact tctggaactg
       61 ggagaacaat ttactaccaa aggatataat taaatttgga tagcactgat ttttatggaa
      121 atagtatgcc tcatagataa ccacttggaa gatagccgcc tggcatctga acacggctta
      181 tcagtgtaca ttgatcaagg taaagagaag atcctctttg acaccggagc cactggtaaa
      241 gcagttgaaa acgccatgac aatgggtgtg gaactggaaa aggtggaatt agccatcata
      301 agccacggcc actacgatca cgggggtggc ttagaagcct tcttggaggt caatagaaag
      361 gcccaggttt acatgtgtca gggtgcagct aaaaaacact tccgtatcga caacgacgta
      421 gtgaaggaca tcggattaga tgaatcagtt ctaaagaaat acagtgaacg tatcacctac
      481 ctagatgaat ttattcaatt gagggatgat ctgtttttga ttacatccat acgccaagtc
      541 catgctgttc cagagggtaa taaattcctg tatgaagaag aaggtttgga attggtccag
      601 gatagtttta ctcatgagtt ggtcatggtt ttaaaacgag aggatggttt ggtggtgtta
      661 accggctgca gccacaatgg catactcaac atcctggacg cggtggtgga aaaatttcca
      721 gaaaccccat taatagcagt gtttggtggc ctgcatctca tgataccacc cgatgactct
      781 gtagtggacc cccgggagtt ggatattatg gcccgtaagt tgatggagta tccaattggc
      841 aggatctaca cgggccactg tactggaatc cggtcctatc aattcctgaa aaggatcatg
      901 ggggaccgta tcaactactt cgccactgga tctagggtaa cgatataaaa aaaatgacaa
      961 ctggtttttg atctcatcaa gatcagtaga ttaagaatac tccaagt
//
LOCUS       DAOQVW010000655         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_227650_length_1007_cov_3.079832, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000655 DAOQVW010000000
VERSION     DAOQVW010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227650_length_1007_cov_3.079832"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1007
                     /locus_tag="PKK85_10405"
     CDS             <1..>1007
                     /locus_tag="PKK85_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HNS26467.1"
                     /translation="DENPEELYDLLVLNEKNLQFRPREYESLDRSVMEPAPESQEVDE
                     PSDPLGGSSPEDEEELYPADGEDLPSPGEQADEHLETDSQEGHLDSEKIKPDTGDSAL
                     DLVDELSLDDEGEESQDRIVLKEVYDARGKTLSLDVMDLEEDLHLTKEESDLLWELPP
                     SEGQVDKRIRDLFMQTDLSARELQKRLFRIHQHARTVFEEQGYNVLYLALGFLEWTET
                     SGDQHRAPLILIPVQLERRGVGGAYKLSWNGEDIIPNISLQAKLQEQYITIPDLEMPH
                     RSEGITQYLEEVEDSIPNAPGWQVHREVHLGFFSFTKFVMFQDLDPDHWPPELSFDKN
                     P"
BASE COUNT          276 a          284 c          268 g          179 t
ORIGIN      
        1 gatgaaaacc cggaagaact ctacgatcta ctagttttaa atgagaaaaa tctccagttc
       61 cgtccccgtg aatacgaatc cctggatcgg agtgtgatgg aaccagcacc agaaagtcag
      121 gaggtggatg aaccatcaga tccacttgga ggatcatccc ctgaggacga ggaagaacta
      181 taccctgcag atggagagga cttaccttcc cctggtgagc aagccgatga acacctggaa
      241 acggactccc aggagggaca cctggatagt gaaaagataa aacccgacac aggggacagt
      301 gccctggact tagttgatga gctatccctg gatgatgagg gggaagaatc ccaggatcgg
      361 atcgtcctca aggaggtcta cgatgcccgg ggaaaaaccc tatccctgga tgtcatggac
      421 ctggaggagg acctccacct aaccaaggag gaatccgacc tcctatggga gctcccaccc
      481 tccgagggcc aggtggacaa acgtatacgg gacctgttca tgcaaaccga cttatctgcc
      541 cgggaattgc agaaacgact cttcaggata caccaacacg cccggaccgt cttcgaggaa
      601 cagggataca acgtactcta cctggccctg ggatttctgg aatggacaga gaccagtggt
      661 gaccagcacc gggcacccct gatcctgata ccagtccagc tggaacgccg cggtgtaggg
      721 ggggcctaca agctctcctg gaatggtgag gatatcatcc ccaacatctc cctccaggcc
      781 aaactacagg aacagtacat caccatacca gacctggaga tgccccaccg cagtgaagga
      841 ataacccagt acctggagga ggtggaggat tccatcccca atgcaccggg ctggcaagta
      901 caccgggagg tgcacctggg ctttttcagc ttcaccaagt tcgtcatgtt ccaggacctg
      961 gacccggacc actggccacc agaattatcc ttcgacaaga accccat
//
LOCUS       DAOQVW010000656         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_227678_length_1007_cov_2.776261, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000656 DAOQVW010000000
VERSION     DAOQVW010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227678_length_1007_cov_2.776261"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..>1007
                     /locus_tag="PKK85_10410"
     CDS             189..>1007
                     /locus_tag="PKK85_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein HtpX"
                     /protein_id="HNS26468.1"
                     /translation="MSVELNTIETELSEGHLEEALDFIKEYYLLPQPEKFPQIRMYYR
                     DARKVLSYQVRGDKDDWEVEVQIVARQPFQVKLTPTRSDIQKVVDRLSEELFIAIQYF
                     EDLIRQSTLYFAWVEGEQIIPEKPPTQRKKTSFKIFGSNMLVIYLLFFGVNIVLFLLL
                     GMYAAVAAILGVQLIIVLLSNRILDKTADWTITPENPRVHILEYQLPVDDFKDFLKKF
                     GKDKVLKMKNEIYQRSLAQGQEPTCEMGEEVFQKYGFHCQAQDKVSKVVDVYRIV"
BASE COUNT          253 a          252 c          258 g          244 t
ORIGIN      
        1 tttagacagg cccctgatca gtaattggcc tagtcgctgg ttgtcaggat agttctctga
       61 gaatagtttc ataaaaaaag gttctatcca cctaagcaga aatatttcac ggttttcata
      121 actccccgtg tgtttgttca tacttgtccg gttttaaata actaaggcgc acataagaat
      181 agatagttat gtcagtcgag ctcaacacca tcgagaccga actctctgag ggacacctag
      241 aagaggccct ggacttcatc aaggagtatt atctcctgcc ccagccggag aagtttcccc
      301 agatcaggat gtactaccgg gatgcccgga aagttttaag ttaccaggtc cggggtgata
      361 aggatgattg ggaggtggag gtgcagatag tagcccggca acccttccag gtgaagttaa
      421 cccccacccg atccgatatc cagaaggtgg tggatcggct ctcggaggag ctgttcatcg
      481 ccatccagta cttcgaggac ctgatacgcc agtccaccct gtactttgcc tgggtggagg
      541 gtgagcagat cataccagag aagcctccca cccagcgcaa gaagacctca ttcaagatct
      601 tcggcagcaa catgctggtg atctacctcc tctttttcgg tgttaacatt gtactcttcc
      661 tcttactggg catgtacgcc gcagtagctg ctattctggg tgtgcagctg atcatagtcc
      721 tcttatccaa ccgtatcctg gataagactg cggactggac catcacccct gagaatcccc
      781 gggtgcacat cctggagtac cagctcccgg tggatgattt caaggatttc ctgaagaagt
      841 tcggcaagga taaggtcctg aagatgaaaa acgagatcta ccagaggagt ctcgcccagg
      901 gacaggaacc cacctgtgaa atgggcgagg aagtcttcca gaagtacggt ttccactgtc
      961 aagcccagga taaggtgtcc aaggtggtgg atgtctaccg cattgtg
//
LOCUS       DAOQVW010000657         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_228106_length_1006_cov_2.787592, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000657 DAOQVW010000000
VERSION     DAOQVW010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228106_length_1006_cov_2.787592"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..610)
                     /locus_tag="PKK85_10415"
     CDS             complement(71..610)
                     /locus_tag="PKK85_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNS26469.1"
                     /translation="MTKYVALLRGFSPLNPNMRNENLREVFKGLGFKGVKTVISSGNV
                     IFKTSSGNVEELEATIEEALQQKLGFKTTVIIRRKEELESLVDMEPFKGYKDLPNSRL
                     NVTFLKNEPTTEIEFPYHAESKGYIVLGIYDRAICSAVDLTRTKTPDLMLCLEEKFGK
                     AITTRTWKTVGRILKWMNE"
     gene            complement(617..>1006)
                     /locus_tag="PKK85_10420"
     CDS             complement(617..>1006)
                     /locus_tag="PKK85_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNS26470.1"
                     /translation="DPELYKQWIGPRELTTEIDVFESVSGGSWRYIQRDPEGNEYAFH
                     GVNHEVLPPERIIGTFEFEGLPETGHVILQTLKLISLPDNRTRIVDQSVFQSVEDRDG
                     MLQSGMEIGVDESYQQLDDLLEEMQNK"
BASE COUNT          247 a          237 c          199 g          323 t
ORIGIN      
        1 tcttctgcat ttaaaatcac tccttttatt tttccaagtt aatctctaag agaggaagta
       61 aatttttaaa ctattcattc atccatttta agattctacc gactgttttc catgttctgg
      121 tggttattgc tttaccaaat ttttcttcca gacacagcat taggtcaggt gttttcgtac
      181 gggtcagatc aactgcgctg cagatggcac ggtcatatat gcctaaaacg atgtaaccct
      241 tactttctgc atggtaggga aactcaatct ctgtggtggg ttcatttttt aagaatgtca
      301 cgtttaatct ggagtttggt aagtccttat atcctttaaa cggttccata tcaacaagcg
      361 attctaattc ctctttgcgc ctgatgatga cggtagtttt aaagccaagc ttctgttgca
      421 aggcttcttc aatggtagct tccagttctt ctacatttcc agatgaggtt ttgaaaatta
      481 cattgccact ggagataacg gttttaacac ccttaaatcc caggccttta aagacctcac
      541 gcaggttctc gttacgcatg ttaggattta agggactgaa tccgcgcagt aaagccacat
      601 atttagtcat gtaacctcac ttattctgca tttcctctag taaatcatct agttgttggt
      661 aagattcatc aacacctatc tccattccag actgcagcat accgtcccgg tcctccacag
      721 actggaacac ggactggtca actattctgg tcctgttatc aggtagcgat attaatttca
      781 gtgtctggag tatcacatga cctgtctctg ggagaccttc aaattcaaag gtgccgataa
      841 tacgctccgg gggcaggacc tcatggttca ccccgtggaa ggcgtattca ttcccttccg
      901 gatctctctg gatgtacctc cagctaccgc cgctaactga ctcgaacaca tcgatctccg
      961 tggtaagttc ccggggaccg atccattgtt tatagagttc ggggtc
//
LOCUS       DAOQVW010000658         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_228166_length_1006_cov_2.453207, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000658 DAOQVW010000000
VERSION     DAOQVW010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228166_length_1006_cov_2.453207"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKK85_10425"
     CDS             complement(<1..667)
                     /locus_tag="PKK85_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNS26471.1"
                     /translation="MSDLEIEEGLETVLKELDQTKGVESAIIVNSVGKILKHTLKSSG
                     DLSLFGPMAQVIHSSSQRLLGSAGLGKMEKVLLESKKGKSLFLQLKKVHLIILIEKNA
                     NLGMVIMAAHRVSPRIMEITRDIEVDESSLPEMTSAGPVLESMEESIPKEDSLEKTVR
                     ETLTELEEETAGQMGGATVSADVTSDDIQKTDAVPEVEVNAEAQILPDKESTSRVEIS
                     DLVT"
     gene            complement(788..>1006)
                     /locus_tag="PKK85_10430"
     CDS             complement(788..>1006)
                     /locus_tag="PKK85_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNS26472.1"
                     /translation="LDMARENLIKIVEEVPRVVVDHHLLRDLRYAQFLESLRKQSGRD
                     VLVASELIGKEPYLLEARRKELYFPPKK"
BASE COUNT          245 a          241 c          205 g          315 t
ORIGIN      
        1 ccgtcaccaa atcagagatt tctactctgg atgtgctttc cttatcaggg aggatctggg
       61 cttctgcatt aacttccact tcaggaacag catctgtttt ctgaatatcg tcactggtta
      121 catcagcaga aacagttgct cctcccattt gccctgcagt ttcctcttcg agttcagtta
      181 atgtttcacg aacagttttt tccagagaat cctcctttgg aatggattct tccatggact
      241 caagcacagg ccctgcagat gtcatttccg ggagggaaga ttcgtctact tcaatatccc
      301 gggttatttc cataatacgg ggagaaaccc ggtgggcagc cattattacc atgcccaggt
      361 tggcattttt ctcgataagt atgataaggt gaactttttt caactgcagg aatagtgatt
      421 tgcccttttt tgactctaaa agaacttttt ccattttccc caaccctgct gaccccagga
      481 ggcgttggga agaactgtgt atcacttggg ccatgggccc gaataatgat aggtctccac
      541 tggatttgag ggtgtgtttg agaatttttc ccacggagtt gactataatg gcactttcca
      601 ctcctttggt ctggtccagt tctttcagaa cagtttccag tccttcttct atctctaaat
      661 cactcatcag atcacggtaa ttttttcaat aagatggtat cccaaacaca tattcagtgg
      721 atggtgtaat aaaatttttg cagtgaactt attgagatgc cgatccagta tcctatcatg
      781 ctggtcatta ctttttagga ggaaagtaca actccttcct ccgggcttct aagagataag
      841 gctccttacc aattagttct gaggctacca gcacatctct cccagattgt tttctcaatg
      901 attccaggaa ctgggcatag cgcagatccc ttaagagatg atgatccacc accacccggg
      961 gcacctcttc gacgatttta ataagatttt ctcgggccat atccag
//
LOCUS       DAOQVW010000659         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_228999_length_1004_cov_2.256059, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000659 DAOQVW010000000
VERSION     DAOQVW010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228999_length_1004_cov_2.256059"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKK85_10435"
     CDS             complement(<1..488)
                     /locus_tag="PKK85_10435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513098.1"
                     /note="unique DNA polymerase that is similar to eukaryotic
                     polymerases; forms a heterodimer of the small and large
                     subunits; possesses polymerization and 3' to 5'
                     exonuclease activities; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HNS26473.1"
                     /translation="MGYFDTLESETEKLYAIAREARLKGYDIETEPEIPLAKDLAERV
                     EGLVGPEEIAKRIKELEAEGMSREEVAFQITREITKSEVEGKEDSIEVKEAKADQAIR
                     TALAILTEGVVAAPLEGIAKLKIKRNFDGGWYLAVYFAGPIRSAGGTAAAMAVLLGDY
                     IR"
     gene            720..881
                     /locus_tag="PKK85_10440"
     CDS             720..881
                     /locus_tag="PKK85_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HNS26474.1"
                     /translation="MAKKDKKVLPPSGAGLVRYFEEETRGPKITPMQVVVMTIILAAL
                     CLALRYSFS"
BASE COUNT          282 a          212 c          176 g          334 t
ORIGIN      
        1 aaccggatgt aatcacctaa aagcacggcc atagcggctg cagttcctcc tgcgcttctt
       61 attggcccgg caaaatacac agccaggtac caacctccgt cgaagtttct tttaatcttt
      121 aatttggcaa ttccctctaa gggggcggcg actacacctt cagttaatat ggccagggca
      181 gtccttatgg cctgatcagc ttttgcttcc ttaacttcga tactgtcttc cttgccttct
      241 acttcggatt tggtgatctc tctggttatc tggaaagcca cttcttcccg ggacattccc
      301 tctgcttcca gttcttttat cctcttggca atttcctctg gtccaaccaa tccctccacc
      361 ctttcggcca ggtccttagc caggggtatc tctggttcgg tttcgatgtc gtaacctttt
      421 aggcgggctt ctcgagcaat ggcatataat ttttcagttt cagattctaa agtatcgaaa
      481 tatcccatga actttcctca aagtctctat tattgatttt taagtaattt atccacattt
      541 tatgtgttga tatttactat ggtaatggtt aaacttaaaa agttctattt agaaaataaa
      601 tcaaagtata aatagatgga ttttcataat tttacaagaa tatcaaaaat ttacaaggat
      661 ataaaaaaaa tttaaaatta acaaaatagt aatttatcca cactttaagg tgattattta
      721 tggcaaaaaa agataaaaag gtgcttcctc ccagtggggc tggtctggtc cgatatttcg
      781 aggaggaaac cagaggacct aagattacac ccatgcaggt ggtggttatg accatcatac
      841 tggcagcctt atgcttagcc ctgcgatatt cttttagtta aacgaattta cagattaatt
      901 tttctatttt aactatcaaa ctatgaaaat taatttaaaa gttttttcaa ttatccagag
      961 gtttttaaat ggcgatccac cccattgagt ttagatacgg caca
//
LOCUS       DAOQVW010000660         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_229305_length_1003_cov_3.016878, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000660 DAOQVW010000000
VERSION     DAOQVW010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229305_length_1003_cov_3.016878"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..375)
                     /locus_tag="PKK85_10445"
     CDS             complement(118..375)
                     /locus_tag="PKK85_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNS26475.1"
                     /translation="MDVTIHTVERKDNGWLITLRSQVQPSMDREKLRMMEDWEVTLKG
                     DEIYLKNYFDLSEPWEDGTLEYIIESAILEGKWKLNRLQDD"
     gene            complement(386..>1003)
                     /locus_tag="PKK85_10450"
     CDS             complement(386..>1003)
                     /locus_tag="PKK85_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNS26476.1"
                     /translation="YRIVETAASKFGIPTPRIAVSNTMIANAAATGPSPSRGLVLITT
                     GLLVQLEEDEILAVLGHEMGHLVGRDPIILFSIISLEFILRFTILLPLVVLSPLIYLF
                     VALGFIFFVAKFFETRADLLSAMKIGNPQVLARALRKIGYQRLQAERISPTRAPSWIN
                     FDPHPPIYFRIDRLERMKTPMDVKNPLLKSARDVIDGFKRSLGIK"
BASE COUNT          244 a          246 c          283 g          230 t
ORIGIN      
        1 gggcggatcc agtgctggag ctggaataaa aaaactggtg cagaagaaag agaaaggtta
       61 acccaccgaa cccatccaca cccagaagaa atcagttact taggattaac tggtagacta
      121 gtcatcctgt aatctattca atttccactt cccctccaga atggctgatt caattatata
      181 ctccagtgtt ccatcctccc agggctcaga caggtcgaag tagtttttta aatagatctc
      241 atcaccctta agggttacct cccagtcctc catcatcctg agtttctccc ggtccataga
      301 aggctgcacc tggctgcgga gggtgatgag ccaaccatta tccttcctct caacagtgtg
      361 gatggtgaca tccatctaca atcacctatt taatcccaag ggagcgtttg aatccatcta
      421 ttacatcccg ggctgatttt aagaggggat ttttaacatc cataggggtc ttcatccgct
      481 ccaggcggtc gatgcggaag tagatgggtg gatgggggtc gaagtttatc caactgggag
      541 cccgggtagg ggatatgcgc tctgcctgga ggcgctggta gcctatcttg cgcagggccc
      601 gtgccaggac ctgggggttc ccgatcttca tagcggatag taaatccgcc cgggtttcga
      661 agaacttggc cacgaagaag atgaagccca gggccacgaa gaggtagatc agtggggaaa
      721 gaaccaccag tggtaggagt atggtgaagc ggagtatgaa ctccagggag ataatggaaa
      781 agaggatgat agggtcccgg ccaaccaggt gacccatttc atggcccagt accgctagta
      841 tttcatcctc ctccaactgc accaggaggc cagtggtgat taagaccagt ccccggctgg
      901 ggctggggcc ggtggcagcg gcgttggcga tcatggtgtt gctcactgct atgcggggag
      961 tggggattcc gaatttgctg gcggcggtct ccacaatgcg gta
//
LOCUS       DAOQVW010000661         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanobacteriaceae archaeon isolate AMR_MDS_1078
            NODE_229884_length_1002_cov_2.053854, whole genome shotgun
            sequence.
ACCESSION   DAOQVW010000661 DAOQVW010000000
VERSION     DAOQVW010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852421
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanobacteriaceae archaeon (wastewater metagenome)
  ORGANISM  Methanobacteriaceae archaeon
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanobacteriaceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:12:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,054
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 7 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Methanobacteriaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229884_length_1002_cov_2.053854"
                     /isolate="AMR_MDS_1078"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099680"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKK85_10455"
     CDS             <1..467
                     /locus_tag="PKK85_10455"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HNS26477.1"
                     /translation="ITEKELEMKGNLEQRREIMATTHNVRVEALIDSLGYDTALRVGR
                     KALFKAGLKLGREAKERLGVGGSLQDLIRAARILYRVLGIEFEIEESGDRITMVVNKC
                     SLSNYYTAETCNILSAADEGVVQGLNEDIHMNFTLRITEGHPKCVACISHEI"
     gene            497..>1002
                     /locus_tag="PKK85_10460"
     CDS             497..>1002
                     /locus_tag="PKK85_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNS26478.1"
                     /translation="MMKAIVVGTGAGGATAARELANKGFEVLILEAGGEFKPFTRRIS
                     LVEPLRKIGLTRNEKNFSRVIPAYDSVRSSKDLILFRGIATGGSTILSCGNMVRAEHG
                     LKEIDLDLTKEFDELEELIKISTFPQDRWRPVTRNMFESAQELGLEPHLTPKSVDSLK
                     CISCGLCEV"
BASE COUNT          296 a          200 c          267 g          239 t
ORIGIN      
        1 agataaccga aaaggagttg gaaatgaaag gaaacttaga acagcgcagg gaaataatgg
       61 ccaccaccca taacgtgcgt gttgaagctc tgatcgactc attaggatac gatactgccc
      121 tgagagtggg tagaaaagct ctttttaagg ccggtttgaa gctgggccgg gaagcaaaag
      181 agcgcctggg agtgggaggc agtcttcagg atcttataag agcggcacgg atactatacc
      241 gggtcctggg catagagttt gagatagagg agtccggaga ccgaattacc atggtagtaa
      301 ataaatgttc tctatccaat tactacaccg ctgaaacctg taacatactc agcgccgctg
      361 atgaaggggt ggttcaggga cttaacgagg atatccacat gaattttact ttaaggataa
      421 ctgaaggtca tcccaaatgt gtggcctgta taagtcatga gatttaaata ttactcgata
      481 ttagatggag gagttgatga tgaaagcaat agtcgtaggt accggtgcgg ggggcgctac
      541 agctgcccgg gagctggcta ataaaggttt tgaagtattg attttagaag caggtggaga
      601 atttaagcca ttcacccggc gaatttcact ggttgagccc ctccggaaga taggcctcac
      661 cagaaatgaa aaaaacttct cccgggtcat accagcatac gattctgtgc gttcgagcaa
      721 ggacttgatt ctatttagag ggatagccac aggcggttca actattctgt cctgcggaaa
      781 catggtaagg gctgaacatg gactaaagga aatcgacctg gacctgacca aggagttcga
      841 tgaacttgaa gaactgatta aaatcagtac cttcccccag gatagatgga ggccagtgac
      901 ccgtaacatg ttcgaatcgg cacaggaact gggtttagaa cctcatttaa cccctaaatc
      961 tgtggattcc cttaaatgca tttcctgcgg actttgtgaa gt
//
